LOCUS       DAZSBF010000001        46732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZSBF010000001 DAZSBF010000000
VERSION     DAZSBF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.001"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHXWD_000001"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHXWD_000001"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR7619044.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="ACHXWD_000002"
     CDS             973..1341
                     /locus_tag="ACHXWD_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR7619045.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHXWD_000003"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHXWD_000003"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR7619046.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHXWD_000004"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHXWD_000004"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR7619047.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHXWD_000005"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHXWD_000005"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR7619048.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHXWD_000006"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHXWD_000006"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR7619049.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHXWD_000007"
     CDS             complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHXWD_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR7619050.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHXWD_000008"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHXWD_000008"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR7619051.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHXWD_000009"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHXWD_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR7619052.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="ACHXWD_000010"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="ACHXWD_000010"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR7619053.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10636
                     /gene="murP"
                     /locus_tag="ACHXWD_000011"
                     /pseudo
     CDS             9212..10636
                     /gene="murP"
                     /locus_tag="ACHXWD_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHXWD_000012"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHXWD_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR7619054.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHXWD_000013"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHXWD_000013"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR7619055.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="ACHXWD_000014"
     CDS             complement(12998..13573)
                     /locus_tag="ACHXWD_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR7619056.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="ACHXWD_000015"
     CDS             complement(13634..14083)
                     /locus_tag="ACHXWD_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR7619057.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="ACHXWD_000016"
     CDS             complement(14070..14495)
                     /locus_tag="ACHXWD_000016"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR7619058.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="ACHXWD_000017"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="ACHXWD_000017"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR7619059.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTVAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="ACHXWD_000018"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="ACHXWD_000018"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR7619060.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHXWD_000019"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHXWD_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR7619061.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHXWD_000020"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHXWD_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR7619062.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHXWD_000021"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHXWD_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR7619063.1"
                     /translation="MPALDLIRPSVTAMRVIASVNSEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHXWD_000022"
     CDS             complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHXWD_000022"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR7619064.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHXWD_000023"
                     /pseudo
     CDS             complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHXWD_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHXWD_000024"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHXWD_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR7619065.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHXWD_000025"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHXWD_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR7619066.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHXWD_000026"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHXWD_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR7619067.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHXWD_000027"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHXWD_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR7619068.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="ACHXWD_000028"
     CDS             complement(26015..27418)
                     /locus_tag="ACHXWD_000028"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR7619069.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHXWD_000029"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHXWD_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR7619070.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHXWD_000030"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHXWD_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR7619071.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHXWD_000031"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHXWD_000031"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR7619072.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHXWD_000032"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHXWD_000032"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR7619073.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHXWD_000033"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHXWD_000033"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR7619074.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHXWD_000034"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHXWD_000034"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR7619075.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHXWD_000035"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHXWD_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR7619076.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHXWD_000036"
     CDS             complement(31765..34044)
                     /gene="maeB"
                     /locus_tag="ACHXWD_000036"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR7619077.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34333..35283
                     /gene="tal"
                     /locus_tag="ACHXWD_000037"
     CDS             34333..35283
                     /gene="tal"
                     /locus_tag="ACHXWD_000037"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR7619078.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35303..37306
                     /gene="tkt"
                     /locus_tag="ACHXWD_000038"
     CDS             35303..37306
                     /gene="tkt"
                     /locus_tag="ACHXWD_000038"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR7619079.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37402..38445)
                     /locus_tag="ACHXWD_000039"
     CDS             complement(37402..38445)
                     /locus_tag="ACHXWD_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR7619080.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHXWD_000040"
     CDS             complement(38571..39146)
                     /gene="nudK"
                     /locus_tag="ACHXWD_000040"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFR7619081.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHXWD_000041"
     CDS             complement(39214..41193)
                     /gene="aegA"
                     /locus_tag="ACHXWD_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR7619082.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41399..43099
                     /gene="narQ"
                     /locus_tag="ACHXWD_000042"
     CDS             41399..43099
                     /gene="narQ"
                     /locus_tag="ACHXWD_000042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR7619083.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSPPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43263..46376
                     /gene="acrD"
                     /locus_tag="ACHXWD_000043"
     CDS             43263..46376
                     /gene="acrD"
                     /locus_tag="ACHXWD_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR7619084.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHXWD_000044"
     CDS             complement(46475..46534)
                     /gene="ypfM"
                     /locus_tag="ACHXWD_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR7619085.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10694 a        13148 c        12108 g        10782 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt atagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcacctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 tgactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcta
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgtcag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 tggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagagttaac ggaggcaatc acccgcatag
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcatca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctagtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accgggttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acggagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attacgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt attattcgca
    31681 aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg aatattcagg
    31741 gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa ccacggccag
    31801 cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt gaaccggttt
    31861 cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa cgcgcagtaa
    31921 gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg cagaaccttt
    31981 caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgccg catcgccgtg
    32041 catttcacca tcaatcatca gttctggcgc acgttccctg accagttcca gcgcctgacg
    32101 cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca acagagcaac
    32161 gcgcggttca ataccgaaac gacggacagt ttctgccgcc atcaaggtga tctccgccag
    32221 ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt taccactcgg
    32281 cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac caaagacatt
    32341 tttcaccacg ctaaaatgtt catgataatc acccaccgta ccgcaaatca ttgcatcggc
    32401 ttcgccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca gcgcccgctg
    32461 cgcctgttcc tgagtgacgc cgcgacgctt catgatctgg aagtattcgg tccagtactc
    32521 tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg ctttgatctg
    32581 caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa ggatcggttt
    32641 cgccagtccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct cttccccttc
    32701 cggcagaact acgcgcttcg gcgctttgcg agcctgggag aaaatcggct tcataaacag
    32761 gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat cagcaatcgg
    32821 acgcgtcgcc acgcccgact ccatcgccgc tttcgcgacc gcaggagcga tcttaacgat
    32881 caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca gatcctgatc
    32941 gccatacgct gaagccacca cttcgctctg ttccgcatgg gccagttctg caatcgcgcg
    33001 taccgccgcc agtttcatct cttcgttgat ggcggttgcg ccaacgtcca gcgcgccacg
    33061 gaagatgaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag aacgaccggt
    33121 gcagatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt ccggttccgg
    33181 attcgccagc gccagaatca ttggtgcccg agccattttc ttcaccattt cctgggtcag
    33241 cactttcggg ccggaacagc ccaggaaaat atccgcacct tcaatcacat catcgagggt
    33301 acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt ttggctcacg
    33361 gccttgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt gcagacccag
    33421 cgctaccagc agattcatac aggcgattgc tgcggcaccc gcgccggaaa ccaccatccg
    33481 cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg cagtgctgat
    33541 gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct cgcgcagttt
    33601 ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc cgccgaaggt
    33661 cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat caacttcaat
    33721 gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt ccatcaccgg
    33781 tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt tagagatcac
    33841 cgccaccagg ttaccgcggg cggtatattt gtaggctttt aacgggtctt tttcgatttc
    33901 aagacaaggt gcggcaacgc cgggtgagta ggccagcgcc agatcgcgct gcgttgccag
    33961 aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga aatcaagtgc
    34021 actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc acaaataaag
    34081 tgtgtgggca acaccccctg cccacaacgt ggcgcacatt attaccttgc cggagtctac
    34141 agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat aatcctataa
    34201 cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct tttttgatgc
    34261 gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt tacttttcaa
    34321 ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt cgtggcagac
    34381 agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac caatccttcg
    34441 ctgttactca aggctgccgg attatcacaa tatgagcatt taatagacga tgctatcgcc
    34501 tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg tgacaaactg
    34561 gcggtcaatt tcggtgctga aatcctcaaa atcgtacccg gtcgcgtgtc aacagaagtt
    34621 gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca tctggtggac
    34681 ttgtatcagc aacaaggcgt tgagaaatca cgcattctga tcaagctggc ttcgacctgg
    34741 gaaggaattc gcgcggcaga agagctggaa aaagaaggta ttaactgcaa cctgacgctg
    34801 ctgttttctt ttgcacaggc acgggcctgt gcggaagcag gcgtttttct gatttcgccg
    34861 tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc gtatgtggtg
    34921 gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa gcaacaccac
    34981 tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct cgccttaacc
    35041 ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga aaaagtttcg
    35101 ccagtggtac gtaaattaat cccaccttct cagacgttcc cacgcccagc tcccatgagc
    35161 gaagcggagt tccgttggga gcacaatcag tatgcgatgg cggtagaaaa actgtctgaa
    35221 ggcattcgtc tgttcgccgt tgatcaacgc aaactggaag atcttcttgc cgccaaacta
    35281 taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga ttcgcgcact
    35341 cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga tgggcatggc
    35401 tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aaccctaccg acccaacctg
    35461 gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc tctacagttt
    35521 gctgcatctg accggttacg acctgccgct ggaagagctg aagaactttc gtcaactgca
    35581 ttcgaaaacc ccaggccacc cggagattgg ttatacgcca ggcgttgaaa ccaccaccgg
    35641 tccacttgga caaggtttgg cgaacgccgt cgggctggcg atagcagagc gtacactggc
    35701 ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg tgtttatggg
    35761 cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag gcacgctggg
    35821 actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg gtgaaacaga
    35881 aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc atgtgatcca
    35941 tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag cgcaaagcgt
    36001 gaaagataag ccgtcgctga ttatctgtcg tacggtgatt ggctttggat cgccgaataa
    36061 agcaggtaag gaagaggcgc acggcgcacc gctgggggaa gaagaagtgg cgctggcacg
    36121 gcaaaaactg ggctggcacc atccgccatt tgagatccct aaagatattt atcacgcctg
    36181 ggatgcccgc gaaaaaggcg aaaaagcgca gcagaggtgg aatgagaagt ttgccgccta
    36241 taaaaaggct catccgcaac tggcagaaga gtttacccgt cggatgagcg gtggtttacc
    36301 gaaggactgg gagaaaacga ctcagaagta tatcaatgag ttgcaggcga atccggcgaa
    36361 aatcgccacc cgtaaggctt cgcaaaatac gcttaacgct tatggcccct tgctaccgga
    36421 gctgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga aaggttctgt
    36481 ttcgctgaag gaagatccgg cgggcaacta cattcactac ggggtgcgtg aatttggcat
    36541 gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata ccgcgacgtt
    36601 cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga tgaaagcgcg
    36661 gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc cgacgcacca
    36721 ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct ggcgaccgtg
    36781 cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca acggaccgac
    36841 ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg atcaggttaa
    36901 agagattact cgtggcggtt atgtgctgaa agacagcggc ggtaagccag atattattct
    36961 gattgccacc ggttcagaga tggaaatcac cctgcaagct gcggagaaat tagcaggaga
    37021 aggtcgcaat gttcgcgtgg tttccctgcc ctcgaccgat attttcgacg cccaggatga
    37081 ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg tggaagcagg
    37141 tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg ggatgacggg
    37201 ttatggggaa tctgctccgg cggataagct gttcccgttc tttggcttta ccgccgagaa
    37261 tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatagt gatcgccgga
    37321 tgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacagcgtc ttatccggcc
    37381 tacatgtccc cgccattttg tttaacgggt gatccacaac gtgggccagg cgtctggccc
    37441 atgccagtta tcgcaggtgg gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc
    37501 aaagcgccag cgcgcctgaa tgccacaatc gcttaatccg cgccctttcg ctaaggtcac
    37561 cagttcacgc gatttctcat caaatgttgc gttcatcagt tccagttcat tcgtctcctg
    37621 cccgttgttg aacggcaaac gcaaccgaac cgggcgcgag gctagcggct ttttacgcga
    37681 cacaatccat gccaaatcaa tggtgttata ggcccctgcc tcacagctga tcatcatcag
    37741 cgctttatca tcagtcagcg cagtgacatt cacctcacga cgtaacggat caagcgagca
    37801 gcgcagacca ttcatccgcc agtttccata atccagcaaa tcgttgcgtt cttcgagtga
    37861 gagtggcgtc ggcgttgggt taaccaccgc gacctctttc agcgcaggcg cgggcggtac
    37921 gctgagcggc ggttcgtccc ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg
    37981 ctgagcatca ataaacaaca acgccgcttt taagccactc agagaaatgg tctgattgcc
    38041 gtcgcgtaag gtgatcgcct tcccttcctg aatcatctgc aaaaacgcgg tgatggttgc
    38101 cgtatcatcg gttactaata accatggtga aatccgccac ttgtcaccac ttaacgccaa
    38161 cggctcacca tctaacagca gccgtggcgc tatctctcct tcagacgcat ccggcgactt
    38221 caacccgccg cgctcaatac gtaaaacggc atcggtatgc gccccggcgc tgcggctcag
    38281 ggtcatcacc agtccattat gatcgcccgt attgcgcgcc acgcagaagt tttggttatt
    38341 acaggtgacc tgccagtcgg aaaacgcccg ttgcgctagt gccgcccaca ccaaagacgt
    38401 cggcaacaaa gcgaaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc
    38461 agaagttcaa acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatca
    38521 gatttgtcgg attgagccaa taataaacaa cgaaatatta ttgttatttt tcagtccatt
    38581 aaatgtgacg tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg
    38641 gttttgatca tctccagcgc ctggctgaac ggcagctcaa gcacttcaat atcttcatct
    38701 tcaacaccgc caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg
    38761 attagctcag tcacaccgcc aggcgacata tacagttcaa ataatttgcg cacttcgccc
    38821 acttcatagc ccgtctcttc aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta
    38881 tccagcagcc cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta
    38941 gcgacacgga actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc
    39001 gtcgcaccat taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg
    39061 cgcgtgagat cgtaagtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct
    39121 ttaatgaggg tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt
    39181 acgctgtgaa gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc
    39241 cagccagtca ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag
    39301 atccgcacca cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg
    39361 gtactggctt tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga
    39421 ctccagccac ggcatcccgt gtggattgaa gccaaacacc ataatcaccg catcggctgg
    39481 catgacaaat tcactgccct caatcggtac aagtcgccgg cgcccctggg catccggctc
    39541 gccaagacgc gtgcgcagga aacgaattcc gcaaacgtga ccttgctcat tcagctcaag
    39601 cgccaccggc tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc
    39661 tttcttcgag cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc
    39721 gccgtggcgc agcgcggtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac
    39781 gacgttgagt ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac
    39841 ctgtttagtg ttggcgatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt
    39901 gggtaaaccc gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg
    39961 ttccaaaagc gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat
    40021 ccccatcgcg ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga
    40081 aggaatgccg aaagtgagca agccaccaat ttctggatgg cgatcgtaca ccgtcaccgc
    40141 cacgccattg cgggtcagaa catccgcaca ggccagccct gccggacccg caccgataat
    40201 cgccacccgc ttgtccactt tggtgacatg gcttaagtca ggacgccaac ctttcgccaa
    40261 cgcctgatct gaaatgtagc gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg
    40321 aatagtacag gcaccttcac acaaacggtc ctgcggacaa acgcgtccgg taatttccgg
    40381 cagggtgttg gtcaggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag
    40441 ttcaatccac tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc
    40501 gccgcattta aggcagcgcg aggcttcccg ttgtgcctga tcggcacgga atggcagata
    40561 aatttcatca aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc
    40621 gggcgttgcc tgcatttgtt cgactttact cattaccggc atttcttgtg cagcggtact
    40681 ggcatgccac ggttgatgct cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc
    40741 ggagagtgcg acgtcagtga ccagttgcag cgcgtccgcc gggcaattct cgacacaggc
    40801 aggaccgttt tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc
    40861 tgccgcgacg ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga
    40921 tttacagcca atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc
    40981 gccattaggg cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact
    41041 acgttgctgc tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac
    41101 atgttgctca tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca
    41161 ttgctgactg ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa
    41221 ccttattctt tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat
    41281 gtgcccagaa taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact
    41341 gactaaattg cgttttagcg aactggaaca ttaatgattt tttgtggaga agacgcgtgt
    41401 gattgttaaa cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct
    41461 gtcgattctt tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga
    41521 ggctatcaat attgccggat cgctgcgtat gcagagttac cgcctgggct acgacttgca
    41581 aagtggcagt ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcatc
    41641 ggtattaacc aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca
    41701 tctgaatgcc aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta
    41761 tcaggccaat attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca
    41821 ctacgctgaa cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat
    41881 tttcacgctg gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa
    41941 tcagctggtt accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga
    42001 taccagtctg ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga
    42061 gctgcataaa ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca
    42121 cgaggccaag cgtcgtctgg aggtgttgta tcaatgttcg caggcgctga acacaagcca
    42181 gattgatgtg cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga
    42241 atatctggag ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga
    42301 attgccgatg cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg
    42361 gcaaaatagt cacgtttcat catcagaacc gctgcttaac agcgtttcgt cgatgctggg
    42421 acgcggtttg tactttaatc aggcgcaaaa gcattttcag cagttattgt tgatggaaga
    42481 acgtgcaacc atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg
    42541 tatccagttg acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat
    42601 catggccgat ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac
    42661 tactttccgc ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga
    42721 tacgttacaa aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc
    42781 gctggatgcg caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc
    42841 gatgaagcac gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa
    42901 tcacacggtt tatatccgtg ataacgggat tggtatcggt gaaccgaaag aacccgaagg
    42961 tcattatggt ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt
    43021 ttcaccacct tccggcggcg gcacgttagt gagtattagc tttcgctctg cggagggtga
    43081 ggaaagtcag ttgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag
    43141 tataaaaggg catgataatt tacattaact cctttttttc tccacgattg gctcgtacct
    43201 tgccgctaca gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt
    43261 taatggcgaa tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt
    43321 gtctgacagg gaccctggcg attttttcat tgcccgttga acaatacccc gatctcgcgc
    43381 caccgaatgt gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca
    43441 ccgtgaccca ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat
    43501 ctcagagcag tggcatcggt caggcatctg tcactttaag ttttaaagca ggcaccgatc
    43561 cggacgaagc cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc
    43621 aggcggtgca aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca
    43681 ttgctttcgt ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca
    43741 gtaatattca ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt
    43801 cgcaatattc catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg
    43861 ctaaagatgt cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg
    43921 gtggtacacc ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc
    43981 tgcaaacacc agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg
    44041 taaggctggg cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc
    44101 gcttcaatgg taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa
    44161 tggcgacagc ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg
    44221 gactggaata caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg
    44281 tggtgaaaac gctgctggaa gctatcgccc tggttttcct cgttatgtat ctgttcctgc
    44341 aaaacttccg cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct
    44401 tctccgtact ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac
    44461 tggcgatcgg tctgctggtg gatgacgcaa tcgtggtggt ggaaaacgtc gaacgtatta
    44521 tgagtgagga aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg
    44581 gggcactggt cgggattgcg atggtgcttt cggcggtatt tgtaccaatg gccttcttcg
    44641 gcggcaccac cggtgccatc tatcgccagt tctctattac cattgttgcg gcgatggtgc
    44701 tgtcagtact ggtagcgatg atcctcactc cggctctgtg tgccacacta cttaagccac
    44761 tgaaaaaagg tgagcatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta
    44821 accgcaacgc cgaacgctac gaaaaagggg tggcgaaaat tctccaccgc agcctgcgct
    44881 ggattgtgat ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga
    44941 cgtcgttctt accgctggaa gaccgtggca tgtttactac ctcggtacaa ttgcccagcg
    45001 gttcaaccca acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc
    45061 atgaaaaaga caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg
    45121 ggcaaaacgt ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga
    45181 ccggcacctc gtttgccatt atcgagcgtg caacaaaggc gtttaacaag attaaagaag
    45241 ctcgcgttat cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg
    45301 atatggagtt gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc
    45361 agttgctggc gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg
    45421 acgacagtcc gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg
    45481 ctatcgacga tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact
    45541 ttatggatcg cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc
    45601 tgccagatga catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct
    45661 ctgctttcgc gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt
    45721 attctgcggt tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg
    45781 atattatgga atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga
    45841 tgtcgtatca ggagcgactt tccggcgcgc aggctccggc gctgtacgcc atttccttgc
    45901 tggtggtatt cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa
    45961 tgctggtcgt gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg
    46021 aaaacgacgt ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg
    46081 ccatcctgat cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag
    46141 cgacgctcca cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta
    46201 tcttcggcgt attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg
    46261 tgggtactgg cgtaatgggc gggatgattt cagccactat tctggctatt tacttcgtgc
    46321 cgctgttctt tgtgctggtg cgccgccgct tcccgctaaa gccgcgcccg gaataagcaa
    46381 taaaaaaggc gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca
    46441 gcgggttctt gctgtttata ctttgtctca ggaattactt acgaagcata acttcgataa
    46501 agtctttcca gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta
    46561 ttctacggaa acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct
    46621 ccggggggac aaatcttatg taaatactat ggtcctgcgg tgatgatttg tatgtgatac
    46681 acagcaacat ttcgagatat tcatacggca tctaatactg atttaattca gg
//
LOCUS       DAZSBF010000002        44871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZSBF010000002 DAZSBF010000000
VERSION     DAZSBF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.002"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(337..879)
                     /gene="ycfP"
                     /locus_tag="ACHXWD_000045"
     CDS             complement(337..879)
                     /gene="ycfP"
                     /locus_tag="ACHXWD_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR7619086.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(902..1927)
                     /gene="nagZ"
                     /locus_tag="ACHXWD_000046"
     CDS             complement(902..1927)
                     /gene="nagZ"
                     /locus_tag="ACHXWD_000046"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR7619087.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1938..2762)
                     /gene="thiK"
                     /locus_tag="ACHXWD_000047"
     CDS             complement(1938..2762)
                     /gene="thiK"
                     /locus_tag="ACHXWD_000047"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR7619088.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2743..3384)
                     /gene="lpoB"
                     /locus_tag="ACHXWD_000048"
     CDS             complement(2743..3384)
                     /gene="lpoB"
                     /locus_tag="ACHXWD_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR7619089.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVKAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3398..3775)
                     /locus_tag="ACHXWD_000049"
     CDS             complement(3398..3775)
                     /locus_tag="ACHXWD_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR7619090.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3778..4137)
                     /gene="hinT"
                     /locus_tag="ACHXWD_000050"
     CDS             complement(3778..4137)
                     /gene="hinT"
                     /locus_tag="ACHXWD_000050"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR7619091.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4471..6660
                     /gene="fhuE"
                     /locus_tag="ACHXWD_000051"
     CDS             4471..6660
                     /gene="fhuE"
                     /locus_tag="ACHXWD_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR7619092.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(6720..8153)
                     /gene="ptsG"
                     /locus_tag="ACHXWD_000052"
     CDS             complement(6720..8153)
                     /gene="ptsG"
                     /locus_tag="ACHXWD_000052"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR7619093.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8448..9245)
                     /locus_tag="ACHXWD_000053"
     CDS             complement(8448..9245)
                     /locus_tag="ACHXWD_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7619094.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9256..10260)
                     /gene="holB"
                     /locus_tag="ACHXWD_000054"
     CDS             complement(9256..10260)
                     /gene="holB"
                     /locus_tag="ACHXWD_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR7619095.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10257..10898)
                     /gene="tmk"
                     /locus_tag="ACHXWD_000055"
     CDS             complement(10257..10898)
                     /gene="tmk"
                     /locus_tag="ACHXWD_000055"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR7619096.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10888..11910)
                     /gene="yceG"
                     /locus_tag="ACHXWD_000056"
     CDS             complement(10888..11910)
                     /gene="yceG"
                     /locus_tag="ACHXWD_000056"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR7619097.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11913..12722)
                     /gene="pabC"
                     /locus_tag="ACHXWD_000057"
     CDS             complement(11913..12722)
                     /gene="pabC"
                     /locus_tag="ACHXWD_000057"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR7619098.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12842..14083)
                     /gene="fabF"
                     /locus_tag="ACHXWD_000058"
     CDS             complement(12842..14083)
                     /gene="fabF"
                     /locus_tag="ACHXWD_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR7619099.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14171..14407)
                     /gene="acpP"
                     /locus_tag="ACHXWD_000059"
     CDS             complement(14171..14407)
                     /gene="acpP"
                     /locus_tag="ACHXWD_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR7619100.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14618..15352)
                     /gene="fabG"
                     /locus_tag="ACHXWD_000060"
     CDS             complement(14618..15352)
                     /gene="fabG"
                     /locus_tag="ACHXWD_000060"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR7619101.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15365..16294)
                     /gene="fabD"
                     /locus_tag="ACHXWD_000061"
     CDS             complement(15365..16294)
                     /gene="fabD"
                     /locus_tag="ACHXWD_000061"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR7619102.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16310..17263)
                     /gene="fabH"
                     /locus_tag="ACHXWD_000062"
     CDS             complement(16310..17263)
                     /gene="fabH"
                     /locus_tag="ACHXWD_000062"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR7619103.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17331..18401)
                     /gene="plsX"
                     /locus_tag="ACHXWD_000063"
     CDS             complement(17331..18401)
                     /gene="plsX"
                     /locus_tag="ACHXWD_000063"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR7619104.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18482..18655)
                     /gene="rpmF"
                     /locus_tag="ACHXWD_000064"
     CDS             complement(18482..18655)
                     /gene="rpmF"
                     /locus_tag="ACHXWD_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR7619105.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18707..19228)
                     /gene="yceD"
                     /locus_tag="ACHXWD_000065"
     CDS             complement(18707..19228)
                     /gene="yceD"
                     /locus_tag="ACHXWD_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR7619106.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            19427..20011
                     /gene="yceF"
                     /locus_tag="ACHXWD_000066"
     CDS             19427..20011
                     /gene="yceF"
                     /locus_tag="ACHXWD_000066"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR7619107.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20123..21082)
                     /gene="rluC"
                     /locus_tag="ACHXWD_000067"
     CDS             complement(20123..21082)
                     /gene="rluC"
                     /locus_tag="ACHXWD_000067"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR7619108.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21655..24840
                     /gene="rne"
                     /locus_tag="ACHXWD_000068"
     CDS             21655..24840
                     /gene="rne"
                     /locus_tag="ACHXWD_000068"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR7619109.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25035..25988)
                     /gene="flgL"
                     /locus_tag="ACHXWD_000069"
     CDS             complement(25035..25988)
                     /gene="flgL"
                     /locus_tag="ACHXWD_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR7619110.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26000..27643)
                     /gene="flgK"
                     /locus_tag="ACHXWD_000070"
     CDS             complement(26000..27643)
                     /gene="flgK"
                     /locus_tag="ACHXWD_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR7619111.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27709..28650)
                     /gene="flgJ"
                     /locus_tag="ACHXWD_000071"
     CDS             complement(27709..28650)
                     /gene="flgJ"
                     /locus_tag="ACHXWD_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR7619112.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28650..29747)
                     /gene="flgI"
                     /locus_tag="ACHXWD_000072"
     CDS             complement(28650..29747)
                     /gene="flgI"
                     /locus_tag="ACHXWD_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR7619113.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29759..30457)
                     /gene="flgH"
                     /locus_tag="ACHXWD_000073"
     CDS             complement(29759..30457)
                     /gene="flgH"
                     /locus_tag="ACHXWD_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR7619114.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30510..31282)
                     /gene="flgG"
                     /locus_tag="ACHXWD_000074"
                     /pseudo
     CDS             complement(30510..31282)
                     /gene="flgG"
                     /locus_tag="ACHXWD_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(31454..32209)
                     /gene="flgF"
                     /locus_tag="ACHXWD_000075"
     CDS             complement(31454..32209)
                     /gene="flgF"
                     /locus_tag="ACHXWD_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR7619115.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32229..33434)
                     /gene="flgE"
                     /locus_tag="ACHXWD_000076"
     CDS             complement(32229..33434)
                     /gene="flgE"
                     /locus_tag="ACHXWD_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR7619116.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33459..34154)
                     /gene="flgD"
                     /locus_tag="ACHXWD_000077"
     CDS             complement(33459..34154)
                     /gene="flgD"
                     /locus_tag="ACHXWD_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR7619117.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34166..34570)
                     /gene="flgC"
                     /locus_tag="ACHXWD_000078"
     CDS             complement(34166..34570)
                     /gene="flgC"
                     /locus_tag="ACHXWD_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR7619118.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34574..34990)
                     /gene="flgB"
                     /locus_tag="ACHXWD_000079"
     CDS             complement(34574..34990)
                     /gene="flgB"
                     /locus_tag="ACHXWD_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR7619119.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35145..35804
                     /gene="flgA"
                     /locus_tag="ACHXWD_000080"
     CDS             35145..35804
                     /gene="flgA"
                     /locus_tag="ACHXWD_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR7619120.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            35880..36173
                     /gene="flgM"
                     /locus_tag="ACHXWD_000081"
     CDS             35880..36173
                     /gene="flgM"
                     /locus_tag="ACHXWD_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR7619121.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            36178..36594
                     /gene="flgN"
                     /locus_tag="ACHXWD_000082"
     CDS             36178..36594
                     /gene="flgN"
                     /locus_tag="ACHXWD_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR7619122.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36634..38169)
                     /gene="murJ"
                     /locus_tag="ACHXWD_000083"
     CDS             complement(36634..38169)
                     /gene="murJ"
                     /locus_tag="ACHXWD_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR7619123.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38279..39202)
                     /locus_tag="ACHXWD_000084"
     CDS             complement(38279..39202)
                     /locus_tag="ACHXWD_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR7619124.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39204..39851)
                     /locus_tag="ACHXWD_000085"
     CDS             complement(39204..39851)
                     /locus_tag="ACHXWD_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR7619125.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39862..40446)
                     /gene="rimJ"
                     /locus_tag="ACHXWD_000086"
     CDS             complement(39862..40446)
                     /gene="rimJ"
                     /locus_tag="ACHXWD_000086"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR7619126.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40682..41890
                     /gene="mdtH"
                     /locus_tag="ACHXWD_000087"
     CDS             40682..41890
                     /gene="mdtH"
                     /locus_tag="ACHXWD_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR7619127.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            41954..42601
                     /gene="grxB"
                     /locus_tag="ACHXWD_000088"
     CDS             41954..42601
                     /gene="grxB"
                     /locus_tag="ACHXWD_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR7619128.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42735..43295
                     /locus_tag="ACHXWD_000089"
     CDS             42735..43295
                     /locus_tag="ACHXWD_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7619129.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43401..44447
                     /gene="pyrC"
                     /locus_tag="ACHXWD_000090"
     CDS             43401..44447
                     /gene="pyrC"
                     /locus_tag="ACHXWD_000090"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR7619130.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44521..44766
                     /gene="dinI"
                     /locus_tag="ACHXWD_000091"
     CDS             44521..44766
                     /gene="dinI"
                     /locus_tag="ACHXWD_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR7619131.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10861 a        12127 c        11165 g        10718 t
ORIGIN      
        1 ttacagcaat gaaaatgttc ttcgctattg agaataacat gaatggtgca ttggtcatac
       61 caaaattgat gttaatcaag ttttgttgct gaaaagatag gcgactttac cgggtgagat
      121 caaaaaacag agtttattgt ttattatttg agcaagtccg gaagggtgat atagggtgac
      181 gcgtatatcg agggcgtttg gtcgtcctga taagatgcgc agcatcgcat caggcattga
      241 tgctcgactg tcggatgcgg cgtaaacgcc ttatccgact tacagaattc ggagcgcatc
      301 aggtaccggc aaggttacct gatgcgacga gggcatttac ccgagggttt tgaacgcctt
      361 aatgcgctgc aaatgcgggg agatattctt gaatttgtgc gtctgctctt cgtcccagac
      421 aatctcgtaa taatgatgca actcttcaga tgtccgctgg ctgttaagcg cttcatcatt
      481 acgcgacaaa atcaccaggc aacgatcgcg attcttctcg cggaagttgg tcacacactt
      541 agtggcaata tcggcatact cttccgggcg atcaatcttg ccttccatgt tctcataagg
      601 gaacaaatta gggttgaaga tcacctggcg gatatcgcaa agaaaaccaa tccgttccgc
      661 ccagtatccg cccagaccaa cgccgcaaat cagcggacgc tcgtcgacgt tcagttgcaa
      721 cattttgtcc acttctttga gcagatgctg catatcatgt ttcggatgcc gcgtactgta
      781 gcttatcaag cgtacatccg ggtcaataaa ctgcaattgt aagactttct cgtggttacc
      841 cggactgtta gagtcaaaac cgtgtaaata gataatcatc gcttcctcac ataagccagg
      901 gttagtgacc tgctttctct tcctgccagc gttcatgtaa ctgattcaga cgggtgctga
      961 tcgccttcca gcgagccgag tccatcagtt cctgtcgcga aaatgaacct ttatgataca
     1021 aacgtgtaac acgttctgcc ttgatcggtg acagattatc taacacgctg acggcccctt
     1081 tacgattatt gcagaccagg atcatatcgc aacctgcgtc cagtgatgcc tgcccgcgtt
     1141 cggcataact gcccataatc gcggcacctt ccatcgataa atcgtcagag aaaattacac
     1201 cgtcaaaaca cagttcctga cgcaaaacgg ttttcagcca gtaggaagaa ccgctcgccg
     1261 gacgcggatc aacatcactg tagatcacat gcgcaggcat aatggcgtcg agtttatttt
     1321 cgcggattaa ggaactgaag accgacatat ctttagcgcg aatctccgct tgtggacgtg
     1381 gatcgcacgg tgtttctttg tgtgagtctg ccgttactgc accgtgtcct gggaagtgtt
     1441 tcccggtcgt tttcattccg gcttcatgca taccatcaat aaaccggctg gcaattgcca
     1501 gggctttttg tggatcggca tgataagaac gctcgccaat cgccgcgctg atatgcccga
     1561 catccagcac tggcgcaaag ctgatatcaa tatccatagc gatcatttcg ctggccatca
     1621 gccaacccgc ctcttgcgcc agtttgccgc cctcttccat tcccaacagc gcagcaaagg
     1681 attgtgctgc cggtaagcgg gtaaaacctt cgcgaaaacg ctgcacgcgt ccaccttcct
     1741 gatctaccgc caccaccaga tgattgcgcg atgctgcgcg gatctggcgc accagttcac
     1801 gtaactgggc aggatcatga tagttacgcg taaagagaat cagccctccc accagcggat
     1861 gcgccagtat ttcacgctct tccgcgtcca gttcgtaacc ttcgacatcc aacattactg
     1921 gacccacact gctctcctta ttgttttatt aatagctgcc gccaggtgtc atcggccagc
     1981 ctgataaatt gttgatcgcc gatttgtcgc cagcggtact caaaccaccc tgctttgagc
     2041 atcagcagcc agggaaacca tcgcctgacc tgacgccata attgcgccgg ataaatcttc
     2101 gcgcgagtgg catagtcatt gaccaattgc cggtgctgtt cagtattttc cacccacacc
     2161 gccgccagtt ccagcgcgat atcaccatct ccggcatact cccagtcgat gagttttaac
     2221 cctgacgcgc tatgcactaa atttccggcg tggacatcca tatgcaatgg acttaagcgt
     2281 aaaggccgtg gttcccgcgc tttgcgcaga cgttttaaca ttcgcagcca acccacagtc
     2341 cgccgcgccg gatcgctttg ctgccagtac agttccagta agggcaacag cgttattcgc
     2401 cagccaaaac gtggttgttg atgaagataa tacagcaagc ctgccagttc gttggtatcc
     2461 ggcaaatacg tttttaccgc gccgggcaga tagtcgacta ccatccagtc acggagatat
     2521 aaatgcggct tcggtgcaat gcttgcgggt agttgtgata aagcccgata ctggcgcaag
     2581 aacgcggact gcggcgcatc aggatcgtgc ggctgacgca caacaaaacg ctggccctga
     2641 tgttcaatga gaaaactacc gccactaagc ccactattaa acgtcgtgac gggatgaaac
     2701 tgcgggaaaa agcgcgacag caattcgtcg cgcgtgatgg gattattgct gcgaaacggc
     2761 acctttacct gaccagataa tttcgcccgt ctgcaccagc atcagctgca tttgtagggt
     2821 cggagcctta acgttgccag aggcgctgga gtacagcacg taatgagcgc cgacattgcg
     2881 ggcaatgcct atggctttac tacgggtacc taaactgtcc tgcggcgaca aacctaactg
     2941 ttgcttagcc atcgacagct gctgggcgga aaccagggta aatttcccgt tattagccag
     3001 cgcatttcgc agcgtttcgg tcgcttctgc ggcattcagc gaaccgttag tacggttgtt
     3061 aacgctatca accagcagga cgctacccgc agtcaccccg tcagccccaa gcatcttact
     3121 gaccatcggc tgcattgcgc cattccagtc ataatggcga atatgcggcg caggcggtgc
     3181 cgtttgatct tcgtgctcaa ttgggcctgg ctgctgcggg atcgtcggca ccgagggcac
     3241 tgtggggaca ggctgttgtg gctcggctgg ttgttccggc gctggtttca cttcttctac
     3301 cggtgcaggt tcacgttgcc ccacacaccc ggcgagaaac atcgccagcg cggtaatcaa
     3361 ggcgtagcga ctcatttttg tcatcaagat tcacccctta caaatataga taaagtctga
     3421 ctttgtgcgc ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa
     3481 tgctaacgct gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt
     3541 aaaaacgata atgaacggta acgggttctt gcctttcgtt ataaagcgtt gaggatgctg
     3601 aaggttgaat atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata
     3661 aagatgactc catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac
     3721 agcccaccag cagtaacaac gccagagaca ccagcccaaa gcatcctttt ctcatcgtta
     3781 cagaccttta tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat
     3841 gtggtaaacc tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc
     3901 ggcaatacct tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg
     3961 ctcatgctca gctgacacgt cgttcacagt cggaatgagg atattcggaa tgatcagaat
     4021 atgcgttggc gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac
     4081 gatatcggag gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac
     4141 attttccttt ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta
     4201 tagagtatgg tcgagttaaa cctgctcttt caaccttaac tcacgatttc ttaagcaaaa
     4261 aatcgttttg ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa
     4321 aagacattct ctgtttggtt tacacgacaa atatgaatgc gcatatttct catttgcatt
     4381 tacaaacaaa attattcgca cactaaatat aaccgctcat caccctcctc cggcatgagc
     4441 ctgacgacaa cataaaccaa gagatttcaa atgctttcaa cacaatttaa cagggataat
     4501 caatatcaag ccatcaccaa accgtcacta cttgccggtt gcatagcact ggcactatta
     4561 ccttctgccg cttttgctgc accagccact gaagaaacgg tgattgttga gggttcagcc
     4621 acagctccag atgatggcga aaatgattac agcgtaacgt ctacctctgc gggtaccaaa
     4681 atgcagatga ctcaacttga tattcctcag tcggtcacta ttgttagcca gcagcggatg
     4741 gaagatcagc agttacaaac gctgggcgaa gtgatggaaa acacgctggg gatcagcaaa
     4801 agtcaggcgg attccgatcg tgctctttat tattcccgtg gattccagat cgataactat
     4861 atggttgatg gtatccccac ctattttgaa tcgcgctgga atctgggcga cgcactttct
     4921 gatatggcac tctttgaacg cgtagaagta gtgcgtggcg cgacaggact catgaccggg
     4981 acgggtaatc catctgcggc aattaatatg gttcgaaaac acgcgaccag tcgtgaattt
     5041 aaaggcgatg tctcggcgga atacggtagc tggaacaaag aacggtatgt ggcggattta
     5101 caaagcccac tcaccgaaga cggtaaaatc cgcgcgcgaa ttgtcggcgg ctaccagaat
     5161 aacgactcat ggctggaccg ctacaacagt gaaaagacct tcttctcggg cattgtcgat
     5221 gctgatttag gcgatcttac gacgctttca gccggttacg aatatcagcg cattgatgtt
     5281 aatagcccaa cctggggcgg tttaccgcgc tggaatactg atggcagcag caacagttac
     5341 gatcgtgcac gcagtaccgc acctgactgg gcgtacaacg ataaagagat caacaaggtc
     5401 tttatgaccc tgaagcagcg gtttgctgat acctggcaag cgacactgaa tgccacccac
     5461 tctgaagtcg aatttgacag caaaatgatg tatgtcgatg cctatgtaaa caaagcggat
     5521 ggtatgctgg ttgggccata cagtaattat ggacctggct ttgattatgt cggcggcacc
     5581 ggttggaaca gtggcaaacg taaagttgat gcgctggatt tgttcgctga cggtagttat
     5641 gaattgtttg gtcgtcagca caatctaatg tttggtggca gttacagcaa acaaaacaat
     5701 cgttacttca gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt
     5761 aatggcaatt tcccacaaac cgactggtca ccacagagcc tggcacagga cgataccaca
     5821 catatgaaat cgttatatgc tgctactcga gtcacccttg ccgatccgct gcatctgatc
     5881 ctcggcgcac gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac
     5941 cacaccacgc cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc
     6001 agctatacct ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct
     6061 ccaatcaccg gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg
     6121 accaccacgt tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca
     6181 cctatccccg gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa
     6241 ggggtggaat ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca
     6301 acgcgctata ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc
     6361 acggttaaaa tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt
     6421 ggtgttaact ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc
     6481 gccgagcaag gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac
     6541 ttctcgttac aggggaacgt caataacctg ttcgacaaaa cctacgatac caacatggaa
     6601 ggttctatcg tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga
     6661 ataacacctg taaaaaaggc agccatctgg cggccttagt ctccccaacg tcttacggat
     6721 tagtggttac ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa
     6781 atcgcctgaa caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca
     6841 gcctgatcca ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg
     6901 aggttagtaa tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg
     6961 gtacctgttg cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt
     7021 gctttaatca gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc
     7081 gggaacagcc acagtttgct gctgttacca gacagaacga taaagtcgat cagaccgtgc
     7141 gagaacgacg taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc
     7201 agaatcgcgt ggatgatgta caggatcggc gcaacgaaca tgaaggagaa ctcgatcggc
     7261 tcggtgatac cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc
     7321 gcgcggtttt ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac
     7381 attttgaaca ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc
     7441 ggaatgtcgc cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg
     7501 aaaggtacgt tccagatgtg gtgcagacca aacggcacca ggcaacgttc gatgaaaccg
     7561 taaatgccaa acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt
     7621 gcagaaccaa tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg
     7681 ccagaaatga tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta
     7741 atacggtaga aacggttaaa catgtacgct gcgatcgcac cggagataat ccctccgagt
     7801 acgccagtat ccgccaggtg tttagaggcg atttcttcag caggtaaatg cagtaccagt
     7861 ggcgcaacca cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg
     7921 gatacgccat cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg
     7981 tttgcaaaga cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag
     8041 ctgaaattcg cggaaccgac gcccagcaga atacctgcga taggcagtac ggataccggc
     8101 agcatcagcg atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag
     8161 agtgctcctg agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct
     8221 cgccgtgtac agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg
     8281 ccgatttgtt tgacggctat cacatttcaa cttcagatca ttggagaagt tgcacatttt
     8341 gacaaaacat atgtcacaat atttcaaacg tggttattca ccgcccaatt ttcggcggtg
     8401 aactttactc gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat
     8461 tgaaggcggg aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc
     8521 agttcttcaa cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc
     8581 gcaggttgat tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc
     8641 accagtaacc gatccagggg gacataacgc gcagcatctc gaagttgctc cgcattacgg
     8701 aaggtcacaa tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt
     8761 tctctgtcct ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc
     8821 agaattgcca gcgtatcggc gcgggcgtca cgggtatgga cgataaccgg cttgttcagt
     8881 tcacgaccaa tctggatatg atggatgaag gactcttgct gacgaacttt agtttccggt
     8941 gtgtaataat aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga
     9001 cggcgtaaat cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa
     9061 aatacgacgt tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt
     9121 gtggcgactg ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc
     9181 acgtccttat gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa
     9241 aacatgatgt ctctcttaaa gatgaggaac cggtagcaca acgcccggtt gcaggtaatg
     9301 ctcaatacgc agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg tgacagacat
     9361 taactgttca cgaatgtggc aaacatcccc cagtatagcc tgcaggcgcg agggagaaag
     9421 atggtttgcc agttcgacga caaggcccgg cacatcaaca ttggtcacct gcgcagcacc
     9481 atgatggcgt tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc
     9541 ttgttcatga ttaagggccg ccagcagcga ataccagtcg cccgatggca cgctatatgc
     9601 caatgcctga cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc
     9661 cgcgccaggc gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt
     9721 cacttcgcgt gaaagccagg tcacggcgta ctgttccggc ggcggcgcaa ggtaatgtaa
     9781 ccgacaacga ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa
     9841 ccaggtttct gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt
     9901 cagtaaggca gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt
     9961 cagcttttcg gtgacctcac gtactgcatc aatgcccagc gtatttttcc ctttttcggg
    10021 agccagggtg tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg
    10081 accgcaactt ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat
    10141 taaagcatca tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc
    10201 ggcctgatag ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat
    10261 gcgtccaact ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc
    10321 agcggctggg tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag
    10381 cgggcgcggg tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg
    10441 cgcgcacgcg cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtcg
    10501 gggcgaaaat ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca
    10561 cgtcctccgc cctgatacgc ctgagtggag agatcgtggc gatcgccaat cacccaggtg
    10621 ccgttagcca gagctggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac
    10681 atcagaactt cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc
    10741 aggcttctta acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg
    10801 cggataccca gttgctcaag cgtctcaacc accacattac gcgcggtagt tttgcctgcg
    10861 ccttccagcc cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag
    10921 ataatcctgc acagacttgt tatggctggc aagattggta ttaaacgtgt gaccaccttt
    10981 accatcggcc acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga
    11041 atccgccccc ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata
    11101 cgctgtcggc gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc
    11161 gtaaatcacg gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac
    11221 cgaggcaacc tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat
    11281 cgtcaccagc tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct
    11341 atcgaccgct ttcaccattt tcttgtgcgc tcgcttgagt aacgcgacat cggtggtatt
    11401 ggcggtatac atccaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag
    11461 ttcaagtgcc tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg
    11521 agcctcacgc aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg
    11581 gaactgtgct tctttaccgc tttccagcaa tttcagcatc tcgcgcacgg tcatctgcgg
    11641 tgtaaagcgg taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcaacca
    11701 ttgaaaaacc cgtgggcgat tgatgatctt atcggcataa agctgttcac caagcgccag
    11761 acgtccggtc cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc
    11821 ggcaagatgg cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa
    11881 taacaagata atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct
    11941 aaatattcat ataacgttgc tgacgaaaag gagacatcgc cacaggcacg tacgggcatc
    12001 actggcatta acgcattaca aataaccatc tcatctgcct gcaacgcctc ttccagagag
    12061 gcttgcactt cgacaagctg ataagaggat tgtgccagca aacggataca gaattgtcgc
    12121 ataatgccgt taacacctgc ctgatccaga cgcggcgtat aaactacatt gcccttccgc
    12181 cagaacaaat tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc
    12241 tcatcagcgt ttgtctgctc aagatgagag cggatcaata cttgctcaag ccgattaaga
    12301 tgtttaatac ctgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc
    12361 atcccttcgt tacgcaaacg gtcgtaatgt gcaggataaa ccgtaacgga gagaatccgc
    12421 gttgccggtc cactgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg
    12481 actttcagca caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga
    12541 ggccagaagt cacagaaaat catcaaccgc tgacaagcat cctgtagtcg ctggatatgc
    12601 gccgataaca aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca
    12661 aactgcgttg cccgatcgct tactgccagc gattcctgct tatgaccgtt aattaagaac
    12721 ataccggctc cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg
    12781 gaataaaagc taagaaaaaa ggcccgcaag cggacctttt atatgggtgg gaaatgacaa
    12841 cttagatctt tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca
    12901 gcgtgtattc cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac
    12961 cttcatccgg gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga
    13021 tggagtagat agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg
    13081 tggagcttac caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt
    13141 cagctttatc gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg
    13201 cttcaatgcc tgcatcacgc agagcatttg ccatcgccag agctgcgcct gcgccatttt
    13261 ctggcggtga cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt
    13321 aaattttcgc accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac
    13381 catcgcccag tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt
    13441 tatcattgcg ggtagataat gcacgtgccg cgccaaaacc accaacaccc agcggcgtac
    13501 tggctttctc tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg
    13561 cagcatggcc aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc
    13621 cacgcaggcc atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa
    13681 cgaagaatgg gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga
    13741 tcagtccgag gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt
    13801 ccgttatatc aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa
    13861 tgaaggcatc catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct
    13921 ttactaagcc agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga
    13981 tgccactctg accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag
    14041 acaacatgcc cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccagggag
    14101 ggaaaaaatg attctagtgg gacaaaaaga taaaactcag gcggtcgaac gaccgcctgg
    14161 agatgttcac ttacgcctgg tggccgttga tgtaatcaat ggcagcctga acggtggtga
    14221 ttttctcagc ttcttcgtcc ggaatctcag tatcaaactc ttcttccaga gccattacca
    14281 gctcaacggt gtcaagagaa tccgcgccca ggtcttcaac gaaagaagca ttgttggtaa
    14341 cttcttcctg cttaacgccc agctgttcgc cgataatttt cttaacgcgt tcttcgatag
    14401 tgctcatact cttaaatttc ctatcaaaac tcgctttcgc gatggttttc gtagtgtata
    14461 aaatgttgaa aaatttgcaa ctaaatcccg gcaggtctta ccacgatttt acgttatttt
    14521 gaggccttac cccccaataa cgcaaatcat tttgcactaa ttattactca taaccacgca
    14581 gactgcgcgc aacatgagca ttttgtgcaa atcgcggtca gaccatgtac atcccgccgt
    14641 tcacatgcaa agtttcaccc gtgatgtaag ctgcttcgtc ggatgccagg aatgcaaccg
    14701 cgttggcgat ttcctgtgcg ccgccgaggc gacccgcagg aacctgcgcc aggatacccg
    14761 cacgctggtc atcgctcagc gcacgtgtca tgtccgtttc aataaagccc ggagcaacaa
    14821 cgtttacagt aataccgcgt gacgcaactt cgcgcgccag tgatttactg aagccgatca
    14881 agcccgcttt cgccgcagcg tagttggcct gaccgccatt tcccatggta ccaaccacag
    14941 aaccgatagt gataatacga ccatgacgct ttttcatcat agcgcgcatt accgcttttg
    15001 acagacggaa aacagatgaa aggttggttt cgataatatc gttccactct tcatctttca
    15061 ttcgcattaa caggttatca cgagtgatac cggcattatt gaccaggata tccacttcac
    15121 caaattctgc gcgaattttt tccagaacag attcgataga tgccgggtcg gtcacattca
    15181 acatcagacc tttgccgttg gcacctaaat aatcgctgat cgcctgagcg ccattttcac
    15241 tggtcgcagt gccaataact ttcgcgccac gggctgcgag cgtttcagca attgcgcggc
    15301 caattccgcg gcttgcaccg gttaccagtg cgatttttcc ttcaaaattc atgattttcc
    15361 tcttttaaag ctcaagcgcc gctgccatcg ctgaaggttc gttcagcgcc gaggcggtca
    15421 gggtgtcgac aatgcgtttc gtcaggccag taagcacctt gcccgggccg acttcataga
    15481 gatgttctac gccttgcgct gccatgtact caacagactt cgtccactga accgggttat
    15541 acaactgacg taccagtgcg tcacggatgg catcaccatt ggtttcgcat ttcacatcaa
    15601 cgttattcac aacagaaact gttggtgcgt taaaggtgat tttcgctaat tctactgcca
    15661 gtttgtcggc tgctggtttc atcagcgcac agtgagacgg tacgctcact ggtaacggca
    15721 gagcacgttt tgcgcccgca gctttacagg cagcgccagc acgctcaacc gcttctttat
    15781 gaccggcaat aaccacctgt cccggagagt taaagtttac cggagaaacg acctgacctg
    15841 ctgcagcttc ttcacacgct ttcgcaatag acgcatcatc cagaccgatg attgccgcca
    15901 tagcgcccgt accttccggt acggcttctt gcatgagctt gccgcgcatc tcaaccagac
    15961 gcaccgcatc agcgaaatca atcacaccag cgcaaaccag cgcggaatat tcccccaggc
    16021 tgtgaccggc catcattgcc ggtgctttac cgccctgctg ctgccatacg cggtacagcg
    16081 caacagaggc agtcaacagc gctggctggg tttgccaggt tttattcagt tcttcagctg
    16141 gcccctgctg ggtcagcgcc cacaggtcgt agcccagcgc cgcagaagct tcagcaaacg
    16201 tttcttcgac aattggatag ctcgccgcca tatcagccag cattccaacg gtttgagaac
    16261 cctgtccagg gaacacaaat gcaaattgcg tcatgtttta atccttatcc tagaaacgaa
    16321 ccagcgcgga gccccaggtg aatccaccgc caaaggcttc aagcagaacc aactgccccg
    16381 gcttaatgcg cccgtcgcgt acagcttcat ccagcgcgca cgggacagag gccgcagagg
    16441 tattaccgtg gcgatccagc gtcaccacga cattgtccat agacataccg agttttttcg
    16501 ccgttgcact gataatacgc aggttagcct gatgcggaac cagccagtcc agttgagaac
    16561 ggtcaagatt atttgccgcc agcgtctcat caacgatgtg cgccagttcc gttaccgcaa
    16621 ccttgaagac ttcgttgccc gccatcgtca gatgaattga attctctgga ttcacacggt
    16681 cagcattagg cagcgtcagc aactcaccat agctaccgtc ggcatgcaga tgggtggaga
    16741 tgattcccgg ctcttcagag gcagccagca ccgcagcgcc cgcgccatcg ccaaaaataa
    16801 taatagtccc acgatcggtt ggatcgcagg tgcgcgccag tacatcggaa ccgacgacca
    16861 gagcatactt caccgcccca gatttcacgt attgatcggc tacgcttaat gcataggtga
    16921 aacctgcgca ggctgctgca acgtcaaatg ccgggcaacc tttaatgccc agcatgcttt
    16981 gaatctgaca agctgcgctc gggaaagcgt gcgtagcaga agtcgttgcc acaacgatca
    17041 ggccaatctg gtctttctca atgcccgcca tctcaattgc gcgtgtcgcc gcttcaaagc
    17101 ccatggttga aacggtttcg tttggcgcgg caatgtggcg ttcgcggata ccggtacgag
    17161 tgacaatcca ctcgtcagag gtgtccacca ttttttccaa atcggcgttt gtccgcactt
    17221 gttcgggcag atagctgcca gtaccaataa tcttcgtata catgtacgct cagtcacttt
    17281 tcggttatat accgtcactt gcaaactgcg agttcgctgg cagcgtcctg ctaccgcaga
    17341 gttccgcttt tgccaccgtc cagcagctca aaaccagctg ggtatacaga ttccaggcga
    17401 gcggcaattc gctgaggaac ttgtcgctgc accgcctgca ctgcctgttc aatcgcgacc
    17461 gcaaaagctc gctgattggc tgcaccatga ctttttatca ccgtgccgcg caatcctaac
    17521 agacaggcgc cgttatactg gtcggggttg aggtgactga atcgcctcgt caggctcttt
    17581 tgtagccaac gctttaataa cagtagccac cacgaccgtt ttttcccttc accctgagat
    17641 ttcagcagag aaaggaacat cctgacaaca ccttccatcg tctttaatgt gacatttcct
    17701 gtaaagccgt cacaaaccag cacatctgtc ttgccagtta acaactcatt ggcttcaaga
    17761 tagccgatat aattgataga agggattgtt ttaagcaccg ctgaggcatc ccgaatactg
    17821 tcgagaccct ttacttcttc ttcaccaata ttgagcaacg ccacgcgagg attgggaatt
    17881 tccaccacct cttcagccag aactgagccc ataatggcaa attgcaccaa cattgtactg
    17941 tcacaatcga cgttggcccc taagtcaagg accaccgttt tgcccttttg ctgatgtggt
    18001 aataccgtca ccagcgccgg acgctcaatc ccctccaggg gcttgagtaa taattttgcc
    18061 agccccatca gtgccccggt attaccggca ctgacacagg cttgcgctcg accttctttc
    18121 accagctcca gggccacgcg cattgaactc ccacgactgg cgcggatagc ttgcgaaggc
    18181 cgggcatcac tggcgataac tgactgcgca ggaataatct gcagacgcga acgttgttca
    18241 aagtcagctt tagcaagtaa tggcgtgatg gcgtcgggat tgccgactaa aagaagagtg
    18301 agttgcgaat tagaattcag tgcctgcaat gctgcaggca ctgtcacgga agggccaaaa
    18361 tcccctccca tgacatctaa cgccagggtt agacgtgtca aggtatcgtc gccgcccggt
    18421 ttggtctttc cccagttgcc tggggaaaat cctcactaag cttcatcacg cagacgcgtg
    18481 attacttagc gatgaccttg cggccgcggt agtaaccgtc ggcagtgatg tggtgacgca
    18541 ggtgtttttc accagaagtt ttgtctacag acaggctggt gactgcggtc agcgcgtcat
    18601 gggaacgacg catgccacgt ttggaacggg ttggtttatt ctgttgtacg gccatggacc
    18661 ttactcctca attacggttt atccccgatc caacggggag caccaattac ttacgcttta
    18721 agctggctaa tacggcaaat gggtttggct tttgcacttc ttcaggcagt tcaccaaaga
    18781 ccatgtccgc ttcggacact tcacagtgtt cagaatcatg caccggaact accggcaagg
    18841 cgaggatgat ttcatcttca accattgcaa gcagatcgat ttcaccgaat tcgttaacct
    18901 caatcggttc atacgcttcc ggcagtgctt cagcctgttc gtctgaacgc acaggactaa
    18961 aacaatacgt tgtgtagacc tgatgagtaa acggcttccc gcaacgctga cactcgagcg
    19021 ttaccgtcac cttcgcatcg ccgtttaaca ctgcgagacg ttggttatcg atagcgaacg
    19081 acatggagca ttccacatca ctgtccacac tgactacgga ttcggcgacg cgctcaacct
    19141 gatcaggggt atagataccc tggtaatcaa ggcgtttttg agccgtacga accggatcga
    19201 gagtcagggg taattttacc ttttgcatag ggcgcgcata ttaactttgt aacgtcatag
    19261 agtcaaagaa aaaggcagcc tgtggttgcc ttttgccaat aattcgcaca cattgcgggt
    19321 tactgcttta ttttcgttca gtgctgataa cgaacatcaa cctgcaaaac gaagcgcata
    19381 gtttaaaatg gctatcatca atacgctata tctgatggaa aaaaatatgc ctaaacttat
    19441 tttagcctcc acctcgccat ggcgtcgcgc tcttctggaa aaactgcaaa tctcttttga
    19501 atgtgcagca ccagaggtcg acgaaacccc acgcagcgac gaatcaccgc gacagttggt
    19561 gcttcgactg gcacaagaaa aagcgcaatc tctggcgtca cgttatccgg atcatttaat
    19621 tattggttca gaccaggtat gtgttcttga tggcgaaatc accggtaaac cgttaacgga
    19681 agaaaatgcc cgtctgcaat tacgcaaagc cagcggcaat atcgtcacct tctatactgg
    19741 actggcgttg tttaattcgg cgaatgggca tctacaaaca gaagtggagc cttttgacgt
    19801 ccatttccgt cacctgagcg aggcggagat tgataattac gtgcgtaagg agcatcccct
    19861 gcactgcgcg ggtagcttta agagtgaagg atttggcatt acgctgtttg agcgtttaga
    19921 gggacgtgac cctaacacgc tggttggttt accgctaatc gcgctgtgtc agatgttacg
    19981 tcgggaaggg aaaaacccgc tgatgggata gttgtttgcg ctggatgcgg ttacgtcgca
    20041 tccggcaatc ggtgcaggcg gcctgatgcg gcgtaaacgc cttatcaggc caaccgccca
    20101 ttatcaggct tatatgcttg tattagcgcg cgttacgcag cttttgcaaa caacgcttca
    20161 aaccttcatc catcggcgct tcgatacgca tcacctcacc ggtccccgga tgggtaaact
    20221 tcaacgctgc ggcgtgcagg aacagacgat ttaatcccgt gcctgcttca gtgagctgtc
    20281 tgtcaaattc acggtcaccg tagcgatcgt caaaggcaat cggatgaccc gcatactgtg
    20341 tatgcacacg gatctgatga gtacgtccgg ttaccgggct acaacgcacc agggtggcaa
    20401 atgcatagcg ttcttccact ttaaagcgtg tttccgacgg tttgccttcc tgactgacac
    20461 gcacgatacg ttcgccgctt tgcagaatat ttttcaataa cggcgcttga acgctcttca
    20521 catgcgactg ccactgaccg cgcaccagcg ccaggtaatc tttttgcatt cctttttcac
    20581 gtaattgctc atgcagagaa cgcaacgccg agcgtttttt cgctatcagc aacacacctg
    20641 aagtgtcccg gtcgagacga tgaaccagtt caaggaaccg cgcttccggg cgcaacgccc
    20701 gcaaaccttc gataacgccg aagcttaaac cactgccgcc atgtaccgcc gtaccggaag
    20761 gtttattcag caccaggata tgatcatctt catataagat gacgtccgcc agcgccgcca
    20821 ccttttgcag atgtggcgaa accgcctctt cttcccgctc agcaacgcga accggtggga
    20881 tacgcacctc atcacccgct tcgagtttat attcaggctt aatacgtttt ttgttcaccc
    20941 gcacttcgcc tttacgcaaa atacggtaaa tcatactttt tggtacgcct ttcaattggg
    21001 tacgcaaaaa gttatcgata cgttgccccg cttcgtcagc ggtgatagca acaattttta
    21061 cggatggagt ctctgttttc atggtgggcg attctaatta gccaacagga ttcgcgccac
    21121 tcatttttct atgcttatat ttagttttgc accttattac ttcactacgt gatcacttta
    21181 ttgatggtta ttaaaccaat caccagcaag aagtgaaaaa actgtgagta agcgggtgat
    21241 aaatggtaaa agtcatcttg ctataacaag gcttgcagtg gaataatgag gccgtttccg
    21301 tgtccatcct tgttaaaaca agaaatttta cggaataacc cattttgccc gaccgatcat
    21361 ccacgcagca atggcgtaag acgtattgat ctttcaggca gttagcgggc tgcgggttgc
    21421 agtccttacc ggtagatgga aatatttctg gagagtaata cccagtctgt ttctttgata
    21481 attgcgctgt ttttccgcat gaaaaacggg caaccgacac tctgcgcctc tttgagctga
    21541 cgataaccgt gaggttggcg acgcgactag acacgaggcc atcggttcac acccggaaag
    21601 gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata atgagtaagt tacgatgaaa
    21661 agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg ttgcccttgt agatgggcag
    21721 cgtctttatg acctggatat cgaaagtcca gggcacgagc agaaaaaggc aaacatctac
    21781 aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg cttttgttga ttacggcgct
    21841 gaacgtcacg gtttcctccc actaaaagaa attgcccgcg aatatttccc tgctaactac
    21901 agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg aaggtcagga agtcattgtt
    21961 cagatcgata aagaagagcg cggcaacaaa ggcgcggcat taaccacctt tatcagtctg
    22021 gcgggtagct atctggttct gatgccaaat aacccgcgcg caggtggcat ttctcgccgt
    22081 atcgaaggcg atgaccgtac cgaattaaaa gaagcactgg caagccttga actgccggaa
    22141 ggcatggggc ttatcgtgcg caccgctggc gtaggcaaat ctgctgaggc gctgcaatgg
    22201 gatttaagct tccgcctgaa acactgggaa gccatcaaaa aagccgctga aagccgcccg
    22261 gctccgttcc tgattcatca ggagagcaac gtaatcgttc gcgcattccg cgattactta
    22321 cgtcaggaca tcggcgaaat ccttatcgat aacccgaaag tgctcgaact ggcacgtcag
    22381 catatcgctg cattaggtcg cccggatttc agcagcaaaa tcaaactgta taccggcgag
    22441 atcccgctgt tcagccacta ccagattgag tcgcagatcg agtccgcctt ccagcgtgaa
    22501 gtccgtctgc cgtccggtgg ttcgattgtt atcgacagca ccgaagcgtt aacggccatc
    22561 gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg aagaaaccgc gtttaacacc
    22621 aacctcgaag ctgccgatga gattgctcgt cagctgcgcc tgcgtgacct cggcggcctg
    22681 attgttatcg acttcatcga catgacgcct gtacgccacc agcgtgcggt agaaaaccgt
    22741 ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa tcagccatat ttctcgcttt
    22801 ggcctgctgg aaatgtcccg ccagcgcctg agcccatcac tgggtgaatc cagccatcac
    22861 gtctgcccgc gctgctccgg taccggtacc gtgcgtgaca acgaatcgct gtcgctctct
    22921 attctgcgtt tgatcgaaga agaagcgctg aaagagaaca cccaggaagt tcacgccatt
    22981 gttcctgtgc caatcgcttc ttatctgctg aatgaaaaac gttctgcggt gaatgcgatt
    23041 gaaacgcgtc aggacggtgt tcgctgcgtg attgtgccaa acgatcagat ggaaaccccg
    23101 cactaccacg tgttgcgcgt gcgcaaaggg gaagaaacgc cgaccttaag ctacatgctg
    23161 ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag aagagttcgc tgaacgtaag
    23221 cgtcctgaac aacctgcact ggcaaccttt gccatgccgg atgtgccgcc agcgccaacc
    23281 ccagctgaac ctgccgcgac tgtcgtagcc ccagcaccga aagctgcaac ggcaacacca
    23341 gcagctcctg cacaacctgg gctgttaagc cgcttcttcg gcgcactgaa agcgctgttc
    23401 agcggtggtg aagaagccaa accgaccgag caaccaacac cgaaagcaga agcgaaaccg
    23461 gaacgtcaac aggatcgtcg caaacctcgt cagagcaacc gccgtgaccg taatgagcgc
    23521 cgcgacaccc gtagtgaacg tactgaaggc agcgataatc gcgaagaaaa ccgtcgtaat
    23581 cgtcgccagg cacagcagca gactgccgag acgcgtgaga gccgtcagca ggctgaggta
    23641 acggaaaaag cacgtaccac cgacgagcag caagcgccgc gtcgtgaacg tagccgccgc
    23701 cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg cgctgaatgt tgaagagcaa
    23761 tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg ttcagccgcg tcgtaaacag
    23821 cgtcagctca atcagaaagt gcgttacgag caaagcgtag ccgaagaagc ggtagtcgca
    23881 ccggtggttg aagaaactgc cgctgccgaa ccaattgttc aggaagctcc agctccacgc
    23941 acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa ctgcaccaga acagcaagaa
    24001 gagaacaatg ctgataaccg tgacaacggt ggcatgccgc gtcgttctcg ccgctcgcct
    24061 cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc gtgacgagcg ttatccaacc
    24121 cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg aactggcctc tggcaaagtc
    24181 tggatccgct atccaattgt acgtccgcaa gatgtacaag ttgaagagca gcgcgaacag
    24241 gaagaagtac aagtgcagcc gatggtgact ggggtccctg tcgccgccgc tgtcgaacct
    24301 gttgttagcg caccggttgt tgaagaagtg gctgaagttg tagaagcccc cgttcagatt
    24361 gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg aagtgattgc cgccgcagta
    24421 actgaacagc cgcaggtgat taccgagtct gatgttgccg tagcccagga agttgcagaa
    24481 cacgcagaac cggtggttga accgcaggaa gagacggcag atattgaaga agttgccgaa
    24541 actgctgagg ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc
    24601 gctgaagtcg cagcagaagt tgaaacggta gctgcggttg aacctgaggt caccgttgag
    24661 cataaccacg ctacggtgcc gatgacgcgc gctccggcac cggaatatgt tccggatgca
    24721 ccgcgtcaca gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt
    24781 ggtcatacgg caacacatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa
    24841 taattagctc aagtaatcaa gccctggtaa ctgccagggc ttttttattt catctttgaa
    24901 tcaacatatt aaaattcatc gaatggcatc cttgctaacc aacaatgcaa aataggcaaa
    24961 aaaatgcccg ccccggcgat cccagagcag gcagacaaaa catacccagt ttcatgatat
    25021 gttttaaagc gaaattattt gttgagctgg aagagcgaca atccctgcat atcggtaaat
    25081 gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc
    25141 cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct
    25201 aatgaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg aacggtcagc
    25261 acattgttca gcgagttttt cagtccgcgg ttggttttat ctaacgccgc agcggcggtt
    25321 tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc
    25381 atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttactggta
    25441 atactgtcga aaattttgtc gcccgtatgt cctatcacca tcgaacgcga agcatcgacc
    25501 tgttgcttaa tactttctgt tccgccgaca taatcaccgt tagcctcact aaacggcgca
    25561 gtctctgttt tataaccggc aaaaatgtag cgcccattac cgtcagtggt gttcgccaga
    25621 ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg gtcatcgtca
    25681 ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat agcagtggtg
    25741 acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt acgcgccagc
    25801 gtgtactggc tgttttgcgc ctgtgcctgg gagagaacaa ccgcttgtga tgcagcaatg
    25861 ggatcatctg aagggttaac gactcgctta cccgtcgaca tctgttcgcc gtacttcatc
    25921 cattctgcct gagaattggt gataccacgc atgttttgct ggtacatcat ctgggtactg
    25981 aaacgcattt tatctcccct tagcgaatgt taatcagcgc atcaaaaatc gcgtttgccg
    26041 tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga tttccgtact
    26101 cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc gtcaccacat
    26161 taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata tcactcacta
    26221 acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttacta ttgctttgca
    26281 gatccagcag ggcctgaccg ttgcggttat cgctatcacc cgcatctttt tcgctcgcca
    26341 tcgctatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg tcacttactg
    26401 gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac tccagaccat
    26461 caaatgccac tttaccgttg gcatccggcg tcaccgtaaa agtggtattg ctggcaaggc
    26521 gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc agtaccacgg
    26581 aggcatcagt taccgtggca ccgatcgcaa cgtcaccttt gtttttcgtg ttttgcagaa
    26641 ccgcgggctt accgatagta aagaaatctt caccagcatc gccgttagca tcaaatccgg
    26701 ctttgtgttg ggtgttgaaa gcctcggcaa atgccagcgc cagttgtcca agcgtattac
    26761 gcgtctggtc cagctcctga gaacggaatg tcagaatgcc gcccagcgac ccggtattca
    26821 gtaatttctc cgggatctca atattgcctg ccgtcccatc aacataagcg acagtcgtac
    26881 gagaagggtc agcgctggaa ggaactgccg ccagttgccg cgccgtactt ccctgaacca
    26941 gtgagtaacc attggccacc gtgatgttat aagtgccgcc atcctgaacg ctgacttcta
    27001 caccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc agattgttag
    27061 gtgacgcccc tgcccccacg cctgtcaggc gcgagatttg atcgttcagg ctggcaattt
    27121 gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg acctgtttgt
    27181 cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct tctgatttcc
    27241 caatcagcgc ctggcgcgct gccgggtctt ccgcgttact caccagcgtt tgcaggctgg
    27301 tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc atattgtcga
    27361 ttttcgacat ctgctcatag cgggcggtca ggccgctact ttgcgtctgc gcagcacgta
    27421 actggttggt aataaacgca tcatactcac gctgcacacc agaaacgtag acgccattgc
    27481 caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg gtttggcggg
    27541 tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac gccgcctggg
    27601 ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt ccttataagc
    27661 cttcaattac agagtattat cggcagcgac ccgccggact tgagttattc agaacagatt
    27721 atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca tctgctggat
    27781 catgttggtg agtttacggg catagtgagg atcggtagca taccccgcgt cctgtagggc
    27841 ctgcgccccc tgctccgcac tcgcggctgt cgtcacggcg gcgtagcgcg ggttacgtgt
    27901 taacagccca acgtaatccg acaatgcttc cagatacgag ctgtagaccc gaaatttcgc
    27961 ttttactttc ttcgcttcgc cattttcata ttcagtcgtg gtgatttcag taactggacc
    28021 tttccagttg ccagaggctt tgacgccaaa caggttatag ctcggctcgc cgttttcgcg
    28081 gcggatttgc cgttgccccc aaccagactc cagcgccgcc tgagcgagga tcaaatgatg
    28141 tggcacaccg ctttgctggc ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc
    28201 tttactgtca cccggcagcg aatcatcgta gttacgtggc acggcctttt gcaccagctg
    28261 cgaaagcgcc tgattttgat aacgcaccac agtttcgagc gggaatttca tcggtgctgc
    28321 tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca tcatctctgc
    28381 aagccccaga cctttgcccg ccgtcatctg ttgggcaatc tgctggtcat acatactggt
    28441 atacaggcga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct
    28501 tttcaacatc atctgcacga acatcccttc cacctgacgg gccaccggac ggatatttgc
    28561 cgccggatct tcgcccgctt tcgcctttag ttcgttgagt gattgcgcat cccaggccgc
    28621 acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc ccgcagacat
    28681 cccgcacttt gcattgattg cagtatggac atcagatcca tcggcgtagc gcccagcgca
    28741 ttgagcgcgc gcaccacgtt attgaggctg gcgctggaac gtacgctttg cagcgaaccg
    28801 ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca cagtctgtcc accaccaaac
    28861 ggtgtatctg gctggctgac attggcctga cgattgactg ttactgagag atttccctgc
    28921 gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag
    28981 ttaatcacta ctttagcgtc ctgcggggtg acattaacct gcatattctg gatatctgca
    29041 aggaaacgga cctgggaact gttgccactt ggtacgcgca cctgaatagt ccgcgcatct
    29101 aacgcggtgg cgctgccata tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc
    29161 atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc aaactggctg
    29221 ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag ttggttcacc
    29281 tgaacactgc taccgccagc ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc
    29341 agcgcataca cctgactgtc aacgccctta agcggcgtca tcaacaacgt acctccacgc
    29401 aagcttttgg catttcccat ggaagaaacc accacatcga tggtttgccc ctgacgtcca
    29461 aacggaggaa gtgactctgt caccattacc gcagcgacgt tttttagctg catattggtg
    29521 cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg tgtggtaaac
    29581 ggcgtctggg ttgtctggtc accggtgcca tccagcccca ccaccagacc atagccaatc
    29641 agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc agcctgagcc
    29701 gccgtcgtaa ccagtagaag aattaatgca gagagaaatt taatcaccac agcctcactt
    29761 acattggcga caggttaagg aagaaacgct gcaaccagcc catattttgc gcttcgttaa
    29821 tgtagccatt gcctacgtat tcaatgcgcg catccgccac ctgagtagac ggtacggtat
    29881 tgctgccgct gatagtgcgt ggattaacca cgccagagaa gcgaataaat tcggtaccct
    29941 gattaatggc aatctgtttt tcacccacca catgcaggtt gccgttgacc agtacctggt
    30001 caaccgtcac cgtcaacgtg ccgctaaagg tattgctggc attggccccg ccctttccgt
    30061 tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac agcccctgca
    30121 aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg gcattcgcag
    30181 aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta tcgccaatat
    30241 tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc tgagcagact
    30301 ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc
    30361 cctgcaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc accaacaagc
    30421 tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt
    30481 aaaccggtga acctgccacc ggttaagcct tagagttgcg tcagtttttg cagcatctga
    30541 tcggtggtgg acaccgcttt actgttgatt tcgtaagcgc gttgcacctg aatcatattg
    30601 accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata cagcagtccc
    30661 gcgccgttca ggcccggcgt gctttcgttc ggtacaccag aggattgcgt ttcggtgtag
    30721 aggttttcgc caatgctctc cagcccggtg tcattcataa aggtggtgag attgagctgc
    30781 ccaacctgaa ccggagctgc ctggccttgt tgggttacgc tgaccacgcc atcacgaccg
    30841 atggtgatac ttaacgcatt cgccggaatg gtgatcgctg gctgcacctg aaaaccacca
    30901 gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt ataggctgat gaaccatctg
    30961 gcaacatcac ctggaaaaag ccctgccctt taatcgcgac atctttgctg ttgttggtct
    31021 gcgacaggtt tccctggctg tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga
    31081 tttgtaatcc ggagggtaag gtggtttgtt cggaagactg tgcccccggc tggcgaatgg
    31141 tttgataaag cagatcttca aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt
    31201 ttgccaggtt gttggcaatg acgtccatat tggtttgctg ggcgtcaagg cccgttttgg
    31261 cgatccataa tgaactgatc ataaaatgtc ctgtagtgga ttagtgacaa agtgcatatg
    31321 tcttcttgcc ggatgcggcg taaacgcctt atccggccta caagtgctcg caaaatcgat
    31381 aaattgcgat tgcccccata gacccgataa acttgcgcat cgggcaactt atacttgtca
    31441 tgtatccttt caattaactc atcgacagca gttggttggc acggcctgcg ttatcatcga
    31501 cgctgctgat caccttcatc tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca
    31561 ttgccgcaac ggcattgacg ttactgcctt ccagaacccc cgacatcaca cgcaaggttg
    31621 gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc tgcgcttaaa cgaaaaatgc
    31681 cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac cagtttcaga cgccctactg
    31741 gcgcaactgt atttgccgga tcgcccggat tgagcgccga gattgtgcca tcggcagcaa
    31801 tagtgatttc cgctccttcc ggcacagcaa ttggccccgc ctcgcctatc acctgatgcc
    31861 cctgaattgt cagttgcccg gtgggatcaa cctgaatgct gccattacgc gtataccctt
    31921 cgctgccgtc agcggtctgc acggccagcc agccatcctg ctgcaacgcg acgtccagcg
    31981 ggcgcgaggt gtaatccatt ttgccgggcg tcatatctgc gcccggcgtt gacgccgtga
    32041 ccaacgtgcg cgtgggcaga gaaagccctt ccactggcac cgcgcgtaaa gcattcaact
    32101 gcgcgcgaaa accgggcgtt gaggcattgg ccagattact ggcggttacc gcctgttgat
    32161 tcagtgtctg gctggctgct cccatcgcgg tataaattgc gtgatccatt gagctatccc
    32221 gtcagcgatt agcgtaagtt aaccagcgtg ttgaggatct ggtcctgagt tttgatggtc
    32281 tgggcgttag actgatagtt acgctgggca acgatcatat tgaccagttc tttactgaga
    32341 tcgacattgg acgcttccaa cgcaccgttg gtcagggtgc caaagttgcc cgtcccggct
    32401 gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc gccttcggat
    32461 gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag ttgtgtttgt
    32521 tcgttggaat agttgccgac aaccgtaccg tcatcattga tttgataact caccagatcg
    32581 cccggtttgt agccattctg ggtggttgcc acaatattgt tagcgccggt attttgctgc
    32641 atggagttga ggaagctcag gctaaacgtg gcgggatctg cgccgttaat tgcgccagtg
    32701 gtaatattct ctgttggatt tgaggtcaga acgccattgg cattaaacac cagcttcatt
    32761 gcaggctcgg ctgtacctgt tggatcactg ctatcctggg tgtagacctg ccagttatta
    32821 tccccggtct tcacaaagta gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    32881 gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg cgtaacagta
    32941 ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt agttttcgct
    33001 gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg aatagtcggc
    33061 ggcgtaccgg ttgccgggta acccgtcagc tgtaaacctt gcatattcac caggttacgg
    33121 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc
    33181 agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt gttggtggtc
    33241 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    33301 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    33361 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    33421 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    33481 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt gccgcggagc
    33541 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc accgttactg
    33601 gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt caacgtaccg
    33661 tcccaggtga aactgtggac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    33721 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    33781 ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc aagaacagtg
    33841 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    33901 tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    33961 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    34021 tttttcagct gcgccaccaa caaagtcaga aaactgcttt gtaagtctgc ggcgttgctg
    34081 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtggtt
    34141 accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc agcatcatgc
    34201 ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc gacatggtgt
    34261 taaccatctc cccgacgaca tcaacgttcg gcatttttac gtagcccttt gcatccgcca
    34321 gcggattacc cggttcataa accagtttgt ccggggcctg actttctata acatcggcaa
    34381 cctttacgcc gcctgtcgca gcacctggtg cagcgttaac ctggaatacc acctgttttg
    34441 cccgatatgg ctgtccatcg ggaccggtca cgctatcagc attcgccaga ttactggccg
    34501 ccacgttcaa gcgctgggac tgggcagtta acgccgaccc ggcgatatca aaaatattca
    34561 gcagtgccat ccgttaattt ccgctctgta aaacgttcat catgcctttg atttgcccgc
    34621 tcaacgcgct aaggctcatc tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc
    34681 gatccatatc gacggtatta ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt
    34741 ctgcggtagg aggcgtcagc gcctgcgccg gaatgtgttg cgttgaggtc atcgtcagtg
    34801 caaccacact ggttgcatcc cgtccacgtt gcatgacttt tttaagttca ctggcaaaat
    34861 cgatatcgcg cgcctgataa ccaggggtat cggcattggc gatgtttgct gccagcactt
    34921 cctgacgctg ggcgcgcaga ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct
    34981 tatcgagcat atctcctccg caggtatcaa aattctgcca tcagcttaaa tgcctttacg
    35041 cgcgcgttat cggcggaata aacgcaaaat gggtcgctat ttatgccgtt gatgaccatt
    35101 gcggacaggt acaattcacg ttgtagaaat ggctgggggc gaaaatgctg gcaataaaac
    35161 gtagcgtggc gatcatctcg atactattca ctccgttaag tgcggcgagt aatctcacat
    35221 cgcaattgca aacctttttt agcgcccagc tcgcgggggt aagtgatgag gttcgtgttt
    35281 ctattcgtac agcgcccaat ctactaccgc cgtgcgagca gccattgctt tcgatgagca
    35341 ataattcccg cctgtggggc aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat
    35401 atttacaggt taatgtacag gccacaggaa attatgtggt tgccgcgatg cccattgtgc
    35461 ggggaggaaa gctggaagct ggcaatgtca aactaaaacg cggacggcag gataccctgc
    35521 caccgcgtac ggtgctggat atcaatcaac ttgttgatgc cgttagcctg cgcgatctat
    35581 cacccgatca acctatccag ttaacccagt ttcgccaggc atggcgggta aaagcgggac
    35641 aacgcgtcaa cgtgatcgcc agcggtgacg ggtttagcgc caacgcagaa ggtcaggcgc
    35701 tgaacaatgc agccgtcaca cagaatgcgc gggtgcgcat ggtatcggga ctggtggtca
    35761 gcggcgttgt tgatgcagat gggaatattc ttataaacca gtaagctgtt aaagattacc
    35821 cgtcccttgc cgataaataa gcaacacatg ataaaagcgc cctcaatgag gaataaacca
    35881 tgagtattga tcgcacttcg cctctgaagc ccgtaagcac cgttcaacca cgcgaaacca
    35941 ctgacgcgtc ggtaacgaac acccgggcgg caaaaacaac agcctccacc agcaccagtg
    36001 tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat atcaatcttg
    36061 aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg gacaccggca
    36121 aaattgccga tgcgctgctc aacgaagcgc agcaagactt gcagagtaac tgatcgtatg
    36181 acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct caaaacggta
    36241 atggatcaag aacagcaaca tctctctatg gggcagatca acggcagcca gttgcaatgg
    36301 attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca gttacgcagg
    36361 aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga aattactgtg
    36421 aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga aggacagatt
    36481 gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac gctatatggg
    36541 gcgaacggtc agacctcaac aacccatcgc ggcggtaaaa agatttcgat ctgaatatta
    36601 tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg caaattcttt
    36661 aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac
    36721 cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg gcatgatatg
    36781 taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac gcaacagaaa
    36841 cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc agtaaagcag
    36901 cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccagcat gtttcaacgg
    36961 accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg caattttcac
    37021 tggcgtttta atgtcctggc gggaataaaa gccaggagcc aacactttca ctacaatcag
    37081 gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc
    37141 ggtaaattta ccgtactgga acagcgaaac ggttaacgga cccgaaagaa tgcccaacgc
    37201 aaccgcactc ggcagcgcca acaggaaaca aagacgcaac ccccagtcca tcaaacggtt
    37261 gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca aaatggtgcc
    37321 aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt aatacatcca
    37381 agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta aggagatctg
    37441 gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca ttgctccggc
    37501 atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt gcggtagctg
    37561 atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca gcaccggtgg
    37621 gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg
    37681 cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca ccagcgacgc
    37741 cagggagatc agcaagatat agggaaaggt aatctttagt agctggctgg tcagggcaaa
    37801 tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg gtgcggcgag
    37861 catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa cataagagac
    37921 aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa tcggtacaaa
    37981 tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa gtttaaaagc
    38041 gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg
    38101 tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca gcgattttaa
    38161 taaattcatc ggtgttctaa tccagaccca cgggcaaaac gcctgcaagg caggcgctga
    38221 aagaggcgaa tagtctacct ggattatggt gaattgctac cgccagatgt tacagggttt
    38281 attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc actgcctgtt
    38341 cgccggcggt ttgcagaact gtctgatttt gcacacattc aatgaagtgc cgcgcacagc
    38401 cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta tgcactacgc
    38461 cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc gcatcgtcag
    38521 tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacag gtggtgatct
    38581 gcaaaggatc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg tcgttagtca
    38641 gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg gtatccacca
    38701 catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgaca ctattgctac
    38761 gatgtttatc cattcttagc gaagctgcgg tagcgagttg cgtttttaac tcaccgtaga
    38821 gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc gccgccagtt
    38881 ccaccagccg ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca cagacatgta
    38941 cacccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg gaatgcacaa
    39001 aaaccgcatc gcaactggcg gcaaggctgg ataacgaatc ggcataagga atgcgccagc
    39061 tttcacaaat tggcagggct ttcgcgcgcg taggcgacca ggctccttgt aacatccagt
    39121 cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct aatcccacaa
    39181 cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga atcaagacgc
    39241 tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggcctgtaa atcaccatca
    39301 accgcgttcg acatatccgt taccgccggc tgatcctcta cctcaccact gaaaagatgc
    39361 atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa aggaccatct
    39421 tcgcgatttg ccagttgttc cagcgtcgac tccacttccg ccatatcgct gaattcatac
    39481 attcgcgcgg cgcggctgcg cagttcaccc ggcgtctggg caccacgcaa taacaacgtg
    39541 gtgattaacg ccacttccgc tgcgctcagt ttcagatcgc caaattctga attacaaaaa
    39601 cgttgttcat atttggtgac ccgattacca aagccactca ccgtgcgcag ataatggcgt
    39661 ttgaccagat tatccagttg ctcctgcact tcagattcac tcagattcat gaccggttca
    39721 cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg gtattgctcc
    39781 ggcgtggtca cctgcttttc cagcaagcaa ccaatcacgc gagcttcaag ggcagtaagt
    39841 tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag ttaatgccgt
    39901 cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat agccttcttt
    39961 ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca tataattagc
    40021 cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg tcagggcttc
    40081 aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat agcaggcatg
    40141 aaaagagcca cgaacaacat tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg
    40201 gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa tcatccccag
    40261 cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct cccagggctt
    40321 gaggaaatgg cgattctctg cgtaataatc cgcaagacgc caggcatcac gatcatgcac
    40381 cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc
    40441 aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt attactatac
    40501 ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat tcacatattt
    40561 gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg
    40621 gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc cgggagggga
    40681 aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca tcgataatat
    40741 gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct tcgttgatca
    40801 aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt ttattcagca
    40861 aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt
    40921 taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg
    40981 gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg atccgccgcg
    41041 ttcggcgctg gtggtgaaac taatccgtcc acaacaacgt tgtcgttttt tctcgctgtt
    41101 gatgatgcag gacagtgccg gtgcggtcat tggcgcattg ttggggagct ggctgttgca
    41161 atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctgt gtgcggcgtt
    41221 caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg ttcgcgaagg
    41281 catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc tggcgggtta
    41341 ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg acgtggctgg
    41401 cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct
    41461 ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg
    41521 gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggtc tgcaacaact
    41581 tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac
    41641 cttaagtgct ttgctggcgg acgcaagagc tcgcggcagc tatatggggt ttagccgtct
    41701 gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt ttgacctggg
    41761 caaatcggcg caccagccag agcttccgtg gatgatgctg ggaattattg gcatcttcac
    41821 tttccttgcg ctgggttggc agtttagtca gaaacgcgca acgcgtcgtt tgctggaacg
    41881 cgacgcctga tttgatactc ccccctgact cctccatact gaaagtgaac tgacagccaa
    41941 atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc tcaaagcccg
    42001 catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca acgacgacgc
    42061 agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa aagatgacag
    42121 ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg acggcaaacc
    42181 gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta
    42241 cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt tttctactcc
    42301 cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt ttgccgacct
    42361 gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg cgctggacaa
    42421 actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata ttcagctatt
    42481 tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc gcgttgctga
    42541 ttaccgcgat aatatggcga aacagacaca aatcaatttg ttatcatcaa tggcgattta
    42601 atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag ttttttctgc
    42661 tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac gcagatttca
    42721 tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc agtggcctgc
    42781 tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt aaccagtcgc
    42841 ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct gacgcccata
    42901 ttgttctgac aaacttgatc agccaaattg gtcgcgaaga gccgaataag gttaccctaa
    42961 ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg accatgaaac
    43021 tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga
    43081 tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc
    43141 ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct tacgtcctgc
    43201 gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga
    43261 agccaggcga aattgtcatt ccgtttacgg attaatgacg agggcgcatt cgcgcccttt
    43321 atttttcgtg caaaggaaaa cgtttccgct tatcctttgt gtccggcaaa aacattcctt
    43381 cagccggagc atagagatta atgactgcac catcccaggt attaaagatc cgccgcccag
    43441 acgactggca ccttcacctc cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca
    43501 gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg accacagttg
    43561 aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg cacgatttca
    43621 ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg gagcgcggat
    43681 ttaatgaagg cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc actaactcca
    43741 gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg
    43801 gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg
    43861 aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag
    43921 tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac ggaaatgaac
    43981 ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg
    44041 gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt caccaacagg
    44101 cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg gattctgcgc
    44161 cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc aacgccccaa
    44221 ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag cactttgaag
    44281 cattctgttc tgtaaacggc ccgcagttct atgggttgcc ggtcaacgac acattcatcg
    44341 aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat gacaccctgg
    44401 tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa tcgtagcttc
    44461 ctgttgtcat taggttattt aacctgtata aataaccagt atattcaaca gggggctatt
    44521 atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctggggc tattgacgcc
    44581 ctggctggcg aactttcccg ccgtattcag tatgcgtttc ctgataatga aggccacgta
    44641 tcggtacgtt atgccgcagc gaataattta tcggttattg gcgcaacaaa agaagataaa
    44701 cagcgcatta gcgaaattct ccaggaaacg tgggaaagcg ccgatgactg gtttgtcagc
    44761 gaataatatg cagtgatttt tgtttttgcc gggtcgcccc ggctttttta ttttacttac
    44821 gaattttgag tacaaaattt ctacacttcc tcaaaataat tgcacaatat g
//
LOCUS       DAZSBF010000003        44003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZSBF010000003 DAZSBF010000000
VERSION     DAZSBF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.003"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHXWD_000092"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHXWD_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR7619132.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="ACHXWD_000093"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="ACHXWD_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR7619133.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3005..3086)
                     /locus_tag="ACHXWD_000094"
                     /pseudo
     CDS             complement(3005..3086)
                     /locus_tag="ACHXWD_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHXWD_000095"
     CDS             complement(3099..3347)
                     /gene="hybG"
                     /locus_tag="ACHXWD_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR7619134.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="ACHXWD_000096"
     CDS             complement(3360..3701)
                     /gene="hypA"
                     /locus_tag="ACHXWD_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR7619135.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="ACHXWD_000097"
     CDS             complement(3694..4182)
                     /gene="hybE"
                     /locus_tag="ACHXWD_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR7619136.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4175..4669)
                     /locus_tag="ACHXWD_000098"
     CDS             complement(4175..4669)
                     /locus_tag="ACHXWD_000098"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR7619137.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="ACHXWD_000099"
     CDS             complement(4669..6372)
                     /gene="hybC"
                     /locus_tag="ACHXWD_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR7619138.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="ACHXWD_000100"
     CDS             complement(6369..7547)
                     /gene="hybB"
                     /locus_tag="ACHXWD_000100"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR7619139.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="ACHXWD_000101"
     CDS             complement(7537..8523)
                     /gene="hybA"
                     /locus_tag="ACHXWD_000101"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR7619140.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="ACHXWD_000102"
     CDS             complement(8526..9644)
                     /gene="hybO"
                     /locus_tag="ACHXWD_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR7619141.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9833..10120)
                     /gene="yghW"
                     /locus_tag="ACHXWD_000103"
     CDS             complement(9833..10120)
                     /gene="yghW"
                     /locus_tag="ACHXWD_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR7619142.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10239..11126)
                     /gene="yghX"
                     /locus_tag="ACHXWD_000104"
     CDS             complement(10239..11126)
                     /gene="yghX"
                     /locus_tag="ACHXWD_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR7619143.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11283..12323
                     /gene="gpr"
                     /locus_tag="ACHXWD_000105"
     CDS             11283..12323
                     /gene="gpr"
                     /locus_tag="ACHXWD_000105"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR7619144.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12363..12857)
                     /locus_tag="ACHXWD_000106"
     CDS             complement(12363..12857)
                     /locus_tag="ACHXWD_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR7619145.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13048..13932
                     /gene="yghA"
                     /locus_tag="ACHXWD_000107"
     CDS             13048..13932
                     /gene="yghA"
                     /locus_tag="ACHXWD_000107"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR7619146.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14092..14517)
                     /gene="exbD"
                     /locus_tag="ACHXWD_000108"
     CDS             complement(14092..14517)
                     /gene="exbD"
                     /locus_tag="ACHXWD_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR7619147.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14524..15258)
                     /gene="exbB"
                     /locus_tag="ACHXWD_000109"
     CDS             complement(14524..15258)
                     /gene="exbB"
                     /locus_tag="ACHXWD_000109"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR7619148.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15510..16697
                     /gene="metC"
                     /locus_tag="ACHXWD_000110"
     CDS             15510..16697
                     /gene="metC"
                     /locus_tag="ACHXWD_000110"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR7619149.1"
                     /translation="MTDKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16837..17496
                     /gene="yghB"
                     /locus_tag="ACHXWD_000111"
     CDS             16837..17496
                     /gene="yghB"
                     /locus_tag="ACHXWD_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR7619150.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17536..18435)
                     /gene="yqhC"
                     /locus_tag="ACHXWD_000112"
     CDS             complement(17536..18435)
                     /gene="yqhC"
                     /locus_tag="ACHXWD_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR7619151.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18629..19792
                     /gene="yqhD"
                     /locus_tag="ACHXWD_000113"
     CDS             18629..19792
                     /gene="yqhD"
                     /locus_tag="ACHXWD_000113"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR7619152.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19897..20724
                     /gene="dkgA"
                     /locus_tag="ACHXWD_000114"
     CDS             19897..20724
                     /gene="dkgA"
                     /locus_tag="ACHXWD_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR7619153.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20924..21850
                     /locus_tag="ACHXWD_000115"
     CDS             20924..21850
                     /locus_tag="ACHXWD_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR7619154.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21901..22158
                     /gene="yqhH"
                     /locus_tag="ACHXWD_000116"
     CDS             21901..22158
                     /gene="yqhH"
                     /locus_tag="ACHXWD_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR7619155.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22201..24420)
                     /locus_tag="ACHXWD_000117"
     CDS             complement(22201..24420)
                     /locus_tag="ACHXWD_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR7619156.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24531..25943)
                     /gene="ftsP"
                     /locus_tag="ACHXWD_000118"
     CDS             complement(24531..25943)
                     /gene="ftsP"
                     /locus_tag="ACHXWD_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR7619157.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26018..26755)
                     /gene="plsC"
                     /locus_tag="ACHXWD_000119"
                     /gene_synonym="parF"
     CDS             complement(26018..26755)
                     /gene="plsC"
                     /locus_tag="ACHXWD_000119"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR7619158.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26988..29246)
                     /gene="parC"
                     /locus_tag="ACHXWD_000120"
     CDS             complement(26988..29246)
                     /gene="parC"
                     /locus_tag="ACHXWD_000120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR7619159.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29792..30274)
                     /locus_tag="ACHXWD_000121"
     CDS             complement(29792..30274)
                     /locus_tag="ACHXWD_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR7619160.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30327..30719)
                     /gene="ygiW"
                     /locus_tag="ACHXWD_000122"
     CDS             complement(30327..30719)
                     /gene="ygiW"
                     /locus_tag="ACHXWD_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR7619161.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30871..31530
                     /gene="qseB"
                     /locus_tag="ACHXWD_000123"
     CDS             30871..31530
                     /gene="qseB"
                     /locus_tag="ACHXWD_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR7619162.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31527..32876
                     /gene="qseC"
                     /locus_tag="ACHXWD_000124"
     CDS             31527..32876
                     /gene="qseC"
                     /locus_tag="ACHXWD_000124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR7619163.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32986..33567
                     /gene="mdaB"
                     /locus_tag="ACHXWD_000125"
     CDS             32986..33567
                     /gene="mdaB"
                     /locus_tag="ACHXWD_000125"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR7619164.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33598..33912
                     /locus_tag="ACHXWD_000126"
     CDS             33598..33912
                     /locus_tag="ACHXWD_000126"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR7619165.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33960..35852)
                     /gene="parE"
                     /locus_tag="ACHXWD_000127"
     CDS             complement(33960..35852)
                     /gene="parE"
                     /locus_tag="ACHXWD_000127"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR7619166.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35881..36462)
                     /gene="yqiA"
                     /locus_tag="ACHXWD_000128"
     CDS             complement(35881..36462)
                     /gene="yqiA"
                     /locus_tag="ACHXWD_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR7619167.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36462..37289)
                     /gene="cpdA"
                     /locus_tag="ACHXWD_000129"
     CDS             complement(36462..37289)
                     /gene="cpdA"
                     /locus_tag="ACHXWD_000129"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR7619168.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37314..37736)
                     /locus_tag="ACHXWD_000130"
     CDS             complement(37314..37736)
                     /locus_tag="ACHXWD_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR7619169.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37737..38366)
                     /gene="nudF"
                     /locus_tag="ACHXWD_000131"
     CDS             complement(37737..38366)
                     /gene="nudF"
                     /locus_tag="ACHXWD_000131"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR7619170.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38571..40052
                     /gene="tolC"
                     /locus_tag="ACHXWD_000132"
     CDS             38571..40052
                     /gene="tolC"
                     /locus_tag="ACHXWD_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR7619171.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40200..40871
                     /locus_tag="ACHXWD_000133"
     CDS             40200..40871
                     /locus_tag="ACHXWD_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR7619172.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40877..42037
                     /locus_tag="ACHXWD_000134"
     CDS             40877..42037
                     /locus_tag="ACHXWD_000134"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR7619173.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42075..42863)
                     /gene="ygiD"
                     /locus_tag="ACHXWD_000135"
     CDS             complement(42075..42863)
                     /gene="ygiD"
                     /locus_tag="ACHXWD_000135"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR7619174.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43006..43779
                     /gene="zupT"
                     /locus_tag="ACHXWD_000136"
     CDS             43006..43779
                     /gene="zupT"
                     /locus_tag="ACHXWD_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR7619175.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43837..44001)
                     /gene="yqiD"
                     /locus_tag="ACHXWD_000137"
     CDS             complement(43837..44001)
                     /gene="yqiD"
                     /locus_tag="ACHXWD_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR7619176.1"
                     /translation="MAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNS
                     TREETAEKVE"
BASE COUNT        10795 a        11831 c        11103 g        10274 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ttttcaccac
       61 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc ccacggtgaa ggtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctgatagat gtttttctgc tcggcaaatt taccgctggt
      241 tttgtccagc acctcttcat gatggctgac caggtcgata ttgctaccac agcgaccggc
      301 aatcggtttc actgcgtaac ctgttttcac caattcatca ttaacagtga aatcggtatc
      361 cagcaggtaa cgatggtgcg ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc
      421 ggggatcacc gtccacagcg gctcaaagac tagcacttcc gggcgcagca atacgtcgat
      481 aagacgcact tcgttttgcg gatgaccggt acggattggc accgcagcaa actcacggtc
      541 gctaacttca cgaatctgat caaacgcggt ttcccacgcc caggttttcc acacgcagtt
      601 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatccaa
      661 tccacgcaag atacgcgttt caaagcccgc ctggtgcagc gcctgctcca taaactgcgc
      721 gtgatagttt tcctcgatat ctttgtcctg catgatatgg acaaacggac gtgcacgact
      781 gtgtttccag gcaccagcca attcgttaat cagcccttcc gccggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
      901 ggagtcggcg ttgtactcgt aaaccttcag gccacgctca tccatgcaga aatccatacg
      961 accagtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcgggatg tcgaacagcg ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg
     1081 aagatacatc aggtgcagct cgttggtggc tttaattagc tcctgctcgg cactctcggt
     1141 aatggtgtag taatgataag gatcctgatt gatcacctga ccgttggcct gcacataggc
     1201 gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 gcgcgctccg ctgattttca gcagctcgcc tgcaatttcc ggctgcggta agctgtattc
     1321 agtatcttcc gtctggatca tccagcccag aatggtggtg tcatcaaaag tgtctttcag
     1381 ggtatagcag ccgttttcga ccaccatctc cagctcacgc gtccactgtt gcccttgcgg
     1441 caacggggaa tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 acccgcgacc ggcgcacgcg gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attaaccact tctcgcagga agcgcagcga gaaaatctcc cacgccatac ccacgtcagt
     1681 aaagaccaca ccgtaattca gaaagagaaa acggcgagca aattcaacgc attgccactt
     1741 gtggcccata tattcgtcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccaa tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgcccgttg gcaaacaccc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc
     2341 tggctgattc gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aactcgaaga tcccggcgct gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa
     2461 tctggttcga tcctgcttta tctggcggag aaatttggct acttcctgcc gcaggatttg
     2521 gcaaagcgta ctgaaacgat gaactggctg ttctggttac agggcgcggc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcgccgatga aaattgagta cgccatcaac
     2641 cgctttacca tggaagccaa acgtctgctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc gtggtttggc
     2761 aatgtggtgt taggcggcgt gtatgatgcc gcagagtttc ttgatgcggg cagttataag
     2821 catgtacaac gctgggcgaa agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt
     2881 gttaaccgca ccaacggacc gctgaatgag cagttgcatg agcgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc
     3001 catccaggcc ggataaggcg ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg
     3061 acgctgccgc gtcttatcag gcctacgaaa gctaatcatc acgcgctggt aatgtcgtaa
     3121 tccatttggc gcagtgcgtc taatgtggct ttggcttcat cttcgtcgat gatgctcatg
     3181 gcaaatccga cgtgtaccag cacccactgg cccagtagat cggcagggtt accttcacaa
     3241 atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc aagctggtga
     3301 atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattat taactccggt
     3361 tattcaactt caatactttt gacgatcagc gaatcgccgg tatcgacacg caaccgctcg
     3421 ccgtgacaga gcggacactg cgcatcgtgc tgatgaatct ccaccacctg gctgcaatcc
     3481 cagcaccaag cctgggcggg tttatagacg atatgtaaat cgcacccttg cgccaccgtt
     3541 ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca ggagagcgcg
     3601 ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc ctgccgttgg
     3661 ataatttcaa cggcgctctg gcaaagagac aactcatgca ttttcgccac tcctgcgacc
     3721 aaacagcagg gcgcgacgcc ctgcgtgtgg tacatccgga ttcgtgactg gcagcgaaag
     3781 gatcattcgt gcgcagtcat ccgtcaggcg ttggccctct tcaatcgaca tgctgtgcga
     3841 aagcggcgac atcagcgaac aggagagata ttgcgaaaca ccgtccagtt cgccaacggt
     3901 aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt tgcgcagcgg
     3961 ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg tgatcacaca
     4021 ccccgtccac tgaccttcga acagcgtaaa atcagaaaca tacaccggca ttgaaggatg
     4081 cagaaaagag agatcgtgca tcgaacgccg ggcaatttct tcaaacgctg cctgtacttg
     4141 cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgaatcgcc tcccgtggga
     4201 tggcttccac gcctgattcg cgcagcgcag ccagaacctg ctcaagcgca ggttcaatca
     4261 ttgcttcaac cgtcggcgtt aagccgatgt gtggctccag cgattccggg atcacgccga
     4321 ccagggtcag ctttttcgga aactcaccgg tgaagcgcag ggccgacaat acgtcggcca
     4381 ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct tcatcccgca
     4441 ggatcatcat cgttcccggc gtgttctttt tcgacacaat ggcatccgca ataatcagat
     4501 gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca tcgaggatct
     4561 caacataatc cggcagaatg tatcgttgct ctaacgcttc gacaatccgc acaccgatgg
     4621 cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt acagaacctt
     4681 cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg ccatgcacgg
     4741 gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcag caaccggtgt
     4801 acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg gaccagagtt
     4861 ccaggttgat ggaacaactg cctggtagtt actgataata ccgtctttaa taaccatcca
     4921 gtgagagagc ataccgcgcg gcgcttcgag gaagccaaca cctttgaact cacccgttgc
     4981 cggaatgttc ggtttcacaa aggtggtgtg atcgcctttg ccgatattgg tgatcagtgc
     5041 actgtattga ttttgcagga tatcctgcaa ttcgcagcag tgaacggtac gaccaataat
     5101 acggcccagc gtggagtgca gctgcgccac ttccagcgtg ttgccagtca gtttctgata
     5161 aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg ccagtttcac
     5221 cagcatatta gccagcggac ccacttctac cgttttgccg tagaaagtcg gtgatttcac
     5281 ccaggaatat ttcccgtcgt cagaccaacc atcataagcc ggaatggtgg taccttccca
     5341 cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct gaatgccttt
     5401 gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat ccgcattctc
     5461 aatgtagccg cccgggaaca ggaagctgcc gtttttactg tcggtcggga attccggcac
     5521 gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga acgcggcaat
     5581 aactgcggta tcaaccttat aaacctgttc aacaaagtcg ctcagcttgt cgatgaaaga
     5641 cttgatgtac atcaggcgct caaggttcag cacgcccaaa ccgtcgaggt tgatcgggtt
     5701 cgcgacacca cctaccgcca ggttctgaat gtgcggcgtt ttaccgccca gcagcgccac
     5761 gacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta ccgcaatcag
     5821 gttcacttcc ggcggcagtt tcatcgccgg gtgaccccag tagccattgg cgaaaatacc
     5881 caactgaccg ctggcaacca gatctttgat cttgttctga actttagtga actcttccgg
     5941 actgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg ttgggtcagc
     6001 ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa agctgataga aatgaacgat
     6061 atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg cgttaaccgg
     6121 aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt gagtggtagt
     6181 acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc ggtttttcac
     6241 gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa cgacgccatt
     6301 ttcgatttcg caatcgatgc gtaaatgccc ctcaatacgg gttaccggat caatagtaat
     6361 tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt taaaggagga
     6421 agtatcggca gtagacgaat gagtacgatg taagcgcaaa tctcaatagc cacaaaacca
     6481 atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccgcc cgggttgaat
     6541 gccaccagcg aataggtcag acgccaggtt gcacaaccta acagtgcgct cagtgctgac
     6601 aggaacagca tgcgggaatc attacgcagc ttcgccacac gcagaacgac cagcgggaag
     6661 agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc aaacgctaac
     6721 gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat cgccagcaac
     6781 acactgatgg tgttggtcag cttgacgaac agactctttt catccggacc gttgccacgc
     6841 agacccgcct gcaccagcga accttcaaag atgacaatcg agaagcccat gatgaacgcc
     6901 gtcagcagcg agaacagcgg caacatttca tagctctgcc acaacggatg caccttgtag
     6961 cccgccgaga tcatcagtga ccccattgaa gactggtgca tggtcggcaa cagcgcgcca
     7021 agtgcaatga tgaagaacat aaccttgttc agacgcttga gagaaacctt ccagcccaga
     7081 cgttcaaaca gtgccggagc aaactccagt gccatcacgc cgatatagat ggtcatacag
     7141 accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta gaagtacggc
     7201 aggttccagt agcgacccac gtcgatagtg atcgacaagc cacccagtga gtaaccaaac
     7261 agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt aaagacgtat
     7321 accgcccacg ccagcgccca gccgccacag gcaaagccgg tgccaatcag caggtcaaac
     7381 gcgatccaca cgccccacgg gaagccgccg ttcaggtcag agacagagcc cagaccgaac
     7441 accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa aatcatgacc
     7501 ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat cgtctcctcc
     7561 gtcgtgatgg tcgtttttgg tgttgcgacg aaccagcacg gttaagcccg ccagcacagc
     7621 cagtggtagc atcatgcctt tatacagggt gtgttgaata ttttcggaac gcgcaccggt
     7681 agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg tcagtaccag
     7741 aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt tcggcaccgt
     7801 atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt cgctgccagg
     7861 cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac cgaaaatcac
     7921 cgcgcccgcc gggcacactt ctacgcagcc aggcagaccg cctttatcga gacgttccac
     7981 acctttctgg ttgcacagct cgcacttatg cagcgcacca aacgggttgt tgtagtcgta
     8041 cttcggcacg ttgtacggac aggcgaccat gcagtaacgg caaccggtgc acacgtcttt
     8101 gtcgtaatgg acaatgccgg ttttcggatc ttttttcagt gcagagaccg ggcacacaga
     8161 gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt agccgttctc
     8221 ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt tattggtata
     8281 cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac gttcagggaa
     8341 gttgatatcc tgacacttgg tgacgcaagc ctggcagcct acgcacaagg tcgagtcgta
     8401 caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat gactgacaga
     8461 cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat tacgtctgtt
     8521 cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc cagttcacgc
     8581 accgccatca cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa accaatagcg
     8641 cctgcagaga cgttgccgcc ctctttagcg ttaacatccg gtttctgtga acgcggagtc
     8701 tgattttcga cgttggcaag ctgatggatg cctttatgga agccgatacc ttcttcgtta
     8761 cagccatagc atgggtgacc aatcgctacc ggccacacgc cgccaacatc gcagaattgc
     8821 agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta caggcaccag
     8881 ccttcacggt ggccttcatc accgaactct ttggcaaaac gaccagcatc gaagtgcggg
     8941 cgacgttcgc agtgttcgtg aatcagacgg ccataggcga aggtcggacg gtttttgtca
     9001 tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgcgag gaagttgtgc
     9061 gggttcggcg ggcagcccgg aatattgata acggttttgc ctggcagaac ttcttgcagg
     9121 ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc agagcaggaa
     9181 ccgatagcga taatcgctgc tgcgccttct gccgctttgc ggatgtgatc cacaatcggc
     9241 tcaccagcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc atccacaacc
     9301 aacacatact gccctttgta cttctcaagt gcgttatgtt tgttctcttc gacctgatga
     9361 ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag cacgaggttt
     9421 tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca ctcctgcgcg
     9481 ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcggccat ctctgcagcg
     9541 gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat gaaatcacga
     9601 cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag ttattccgtt
     9661 gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat cgatgcgcca
     9721 tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc gctaaaacga
     9781 agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt gatcaatttt
     9841 cgaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc tcacgaaaga
     9901 aatccattac catctcttta tccagtccat agcggcgcgt cagaataccc gcttcccagg
     9961 cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct aatacaaaac
    10021 cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca tgcgttgctt
    10081 ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc attcgctata
    10141 tatcgccata tatagcgata ttttaggcaa agaacgactt ttgcgcacgc tattttttca
    10201 tgcttgttta agacgtaatc cagtaagaga taaacctatc aggagagata tttatcgaac
    10261 catttcagtg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg gggcgtggaa
    10321 tcattatgga atccgtgatt aacccccgga tagatatacg cctcataaac cttattattg
    10381 gctttcaacg ccgcctcgta agcaggccag ccctcgttga ttcgggtgtc cagttccgcg
    10441 aagtggagta gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc ctgacgacca
    10501 taaaacggca ccgcgcaggc cagttccgga tacgcgacag ccgccgcgtt cgatacgcca
    10561 ccgccatagc aaaatccggt aatacccact ttgcctgccg cttgtggata gcgttgcata
    10621 aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg ctgttgcagc
    10681 tcacgacctt tatcatcatt tcccggataa cctccaacgg aacttaagcc gtcaggtgcc
    10741 agggcgatat accccgcctt cgccactcgc cgtgccacat cttcgatata cggattcagt
    10801 ccacgattct catgcaccac caccacggct ggcgttttgc cgctcatttt tgcgggtttc
    10861 accagataac cccgtacctc gccgtgacca tttggcgaag gatacatgat gtactcagca
    10921 acaatctccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg cttgagcaaa
    10981 tcaaacaacg ctaatgccgt catcccgccc accgcatact tcgccgcaag gttgaggaac
    11041 tcacgtttcg aaattttccc gtgagcgtaa tagtcgtagt aatccaacaa ctcttgtggg
    11101 aaatctttgg cggttaaacg cggcatcgtt gcactcctca gttggtgttt ttttaagcaa
    11161 agcataagca cgtgtttttg cccagttttt cgtcactctg tgagccagac tacgggatac
    11221 gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg aaaggagagg
    11281 tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcaataccgc tattgcggaa
    11341 aaagcggttt acgcctgccc gcgttatcgc tcggtttatg gcacaatttc ggtcacgtta
    11401 acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttgggc attacgcact
    11461 ttgatttagc caacaattac gggccgcctc caggaagcgc agaagagaac tttggtcgcc
    11521 tgctgcggga ggattttgcc gcttatcgcg atgaactgat tatctctacc aaggctggct
    11581 acgatatgtg gcccggccct tacggctctg gcggttcacg taaatacctg ctcgccagcc
    11641 tcgaccaaag cctgaagcgt atagggcttg agtatgtcga tatcttttac tctcatcgcg
    11701 tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg gtacaaagcg
    11761 gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa aaaatggtcg
    11821 agttgctgca cgagtggaaa attccgcttt taattcatca accttcgtac aatttactga
    11881 accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc gtgggctgta
    11941 ttgcctttac tcctctggct cagggattgc tgaccggaaa atatctcaac ggcattccgc
    12001 aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg aaaatgctta
    12061 ccgaagccaa cctcaacagc ctgcgcttat tgaatgaaat ggcacagcag cgtggacaat
    12121 caatggcgca aatggcgtta agctggttgc tgaaagatga tcgcgtgacg tcggtattga
    12181 ttggtgccag ccgcgcggag caactagagg agaacgtgca ggcgctgaat aatctgacat
    12241 ttagcaccga ggagctggcg cagattgatc agcatatcgc cgatggcgag ctgaatctgt
    12301 ggcaggcgtc gtccgataaa tgacctgtta ataacgggcg cgcgaaccgc gcccgaatca
    12361 gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc agaaagcaca
    12421 aacgtcagat ggataatgac gtaccacatc agtttgttat ccgggacatt tttcgcatcc
    12481 ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc tactttgttt
    12541 ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt ctcggagata
    12601 tccagctgtg agacgaaatt ctcataaccg gaaaacatca ccatcaccag caaaccgcca
    12661 accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc cgccatcgag
    12721 aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag ggcaactaac
    12781 gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata cattgcattt
    12841 tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag tatatcgcaa
    12901 ttgagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt cgcataattc
    12961 tgaggtttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct acccttaaag
    13021 gacgttgaac ctgaagggag aaaaacgatg tctcatttaa aagacccgac cacgcagtat
    13081 tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc gaagatgaca
    13141 ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa agatcgtaaa
    13201 gcactggtga cagggggcga ttccggaata ggtcgcgctg ccgccatcgc ttacgcgcgt
    13261 gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc tcaggatgtg
    13321 aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga tttaagcgat
    13381 gagaaattcg cccgttcgct ggttcacgaa gcgcacaagg cgttaggcgg gctggatatt
    13441 atggcgctgg tcgccgggaa acaggttgcc attccggata ttgcagacct caccagcgaa
    13501 cagtttcaaa agacctttgc cattaacgtt ttcgcgctgt tctggctaac ccaggaagcg
    13561 atccccctgc taccgaaagg tgcaagtatc atcaccactt cgtcaatcca ggcataccag
    13621 ccaagtccgc atttactgga ctatgcggct acgaaggcgg cgattctgaa ctacagccgt
    13681 ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga atattgtcgc gccaggccct
    13741 atctggacgg cactgcaaat ttccggcgga caaacgcagg ataagatccc gcagtttggt
    13801 cagaaaacgc cgatgaagcg tgcggggcaa ccggcggaac tggcccctgt atatgtttat
    13861 ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc acggcgtgtg cggcggcgag
    13921 catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattg tgaattcact
    13981 cttacctgat gtggcgaaaa cgccttatcc ggcctacaaa cgcacgaaga ttcaacatgt
    14041 tgcaatagat tttgtaggcc tgatgagcgt agcgcatcag gcaattctac tttacttcgc
    14101 tttggcggtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat gcagcgtatc
    14161 cattaccttc atcaacgtct cgtaatcgac ggttttgtcc gctcggaaga agatggtggt
    14221 gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat cggtgaccgg
    14281 atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg gtttttccgg
    14341 gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta ccgtcgctaa
    14401 cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa acggcgtcac
    14461 gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca ttgccatcgg
    14521 acattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg cgctggcttc
    14581 cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac ccagcatcgc
    14641 tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag gaatcgctgc
    14701 cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa cgactgccag
    14761 gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc agaccgtacc
    14821 aaacagacca acgaacggag aaatcgcgcc gatggttgcc agatagccgt taccgcggcc
    14881 catttgacga cccactgcgg cgacccgacg ctccagacgg aagctggtac gttctttaat
    14941 accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt cattgagcaa
    15001 atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct ggtttaagga
    15061 acgcgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat tgaagaactc
    15121 tacgctctta ctgaagaaga ttgcccaagt gactacggag gccaaaataa gcccaatcat
    15181 cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa ggtccgtctg
    15241 catcaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct gcgcataata
    15301 atatcaaaat gacgtcgaat tgatagtcgt tctcattact atttgcatac tgccgtacct
    15361 ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca tttgcgaaaa
    15421 ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct agtttagaca
    15481 tccagacgta taaaaacagg aatcccgaga tgacggacaa aaagcttgat actcaactgg
    15541 tgaatgcagg acgcagcaaa aaatacactc tcggcgcggt aaatagcgtg attcagcgcg
    15601 cttcttcgct ggtctttgac tgtgtggaag ccaaaaagca cgcgacgcgc aatcgcgcca
    15661 atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttctcctta caacaagcga
    15721 tgtgtgaact ggaaggtggc gcaggctgcg tgctatttcc ctgcggggcg gcagcggttg
    15781 ctaattccat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc aacaccgcct
    15841 atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta acgacatcgt
    15901 ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac actaaaatcg
    15961 tgtttctgga atcgccaggc tccatcacca tggaagtcca cgacgttccg gcgattgttg
    16021 ccgccgtacg cagtgtggtg ccggatgcca tcattatgat cgacaacacc tgggcagccg
    16081 gtgtgctgtt taaggcgctg gactttggca tcgatgtttc tattcaagcc gccaccaaat
    16141 atctggttgg gcattcagat gcgatgattg gcactgccgt atgcaatgcc cgttgctggg
    16201 agcagctacg ggaaaacgcc tatctgatgg gccagatggt cgatgccgat accgcctata
    16261 taaccagccg tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat gaaagcagtc
    16321 tgaaagtggc tgaatggctg gctgaacatc cgcaagtagc gcgagttaac caccctgctc
    16381 tgcctggcag taaaggccac gaattctgga aacgagactt tacaggcagc agcgggctat
    16441 tttcctttgt gcttaagaaa aaactcagta atgaagagct ggcgaactat ctggataact
    16501 tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc ctggcgaatc
    16561 aaccagaaca tatcgccgcc attcgcccac aaggcgagat cgattttagc gggaccttga
    16621 ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg gacgccggtt
    16681 ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt aattgtgaaa
    16741 agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gaatcaggag tacaataggc
    16801 agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca agatatcatc
    16861 gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt tagcgttgtt
    16921 tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc ctcatttttg
    16981 ccaggcgaca gcttgttgat actggcaggc gcattgattg cccaaggggt tatggatttt
    17041 ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct aagttatatt
    17101 caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca gcttcctgct
    17161 aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc gctgctggct
    17221 ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat ttccggtctg
    17281 ccaaaccgcc gcttccagtt tttcaactgg ttaagtggat tgctgtgggt cagcgtggta
    17341 accagttttg gctatgcctt aagtatgatt ccgttcgtta aacgccatga agatcaggta
    17401 atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt aggcacgctg
    17461 tttgtagtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt cccggctgct
    17521 gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat cttcccccgg
    17581 cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt catagtcgac
    17641 gcgcatcgct gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc gcgccttatg
    17701 cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact taaaattatg
    17761 atggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc tcaggttttc
    17821 ggtgtattta ttctcaatcc gtttcagcac gcggctaatc agactgaagt gagtctggcg
    17881 actgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt acagaatttc
    17941 gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca cgtcgagtaa
    18001 ccgctccgcc gcgcataaaa tctcttctga taacgtggcg gagttaatcc cgctggctgc
    18061 catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct gtaactgcaa
    18121 aatatcgaca ttgagacgca atcctgccag cggcacctct gacgtggcat aggtttcgca
    18181 ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac gaaacacgcg
    18241 ttcattgata taaccgattt tatgcccgga aaagagaatt atgatgccag gctcgtacat
    18301 caccggtgta cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg gcaacagttc
    18361 tgacaaacta ttttctttat ttttcagttt attaacttta tccgccagca agcggcaaat
    18421 ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg attgtgcgca
    18481 attttgtagc atttctccag cactctggag aaataggcaa gacattggca gaaatgagca
    18541 ttgagagcca gggcgctggc gatcacaatg aaaaacatca ggcagatcgt tctctgccct
    18601 catattggcc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca ccccaacccg
    18661 cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc acgatgctcg
    18721 cgtattgatt acctacggcg gcggcagcgt gaaaaaaatc ggcgttctcg atcaagttct
    18781 ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa acccggctta
    18841 tgaaacgctg atgaacgccg tgaaactggt tcgcgaacag aaagtgactt tcctgctggc
    18901 ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg ctaactatcc
    18961 ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta aaagcgccat
    19021 cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg caggcgcggt
    19081 gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg ttcagccggt
    19141 atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg tggctaacga
    19201 cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg ttgatgccaa
    19261 aattcaggac cgtttcgcag aaggcatttt gctgacgcta atcgaagatg gtccgaaagc
    19321 cctgaaagag ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg cgactcaggc
    19381 gctgaacggt ttgattggcg ctggcgtacc gcaggactgg gcaacgcata tgctgggcca
    19441 cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg tcctgcctgc
    19501 actgtggaat gaaaaacgcg agaccaagcg cgctaagctg ctgcaatatg ctgaacgcgt
    19561 ctggaacatc actgaaggtt ccgacgatga gcgtattgac gccgcgattg ccgcaacccg
    19621 caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc tggacggcag
    19681 ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac tgggcgaaaa
    19741 tcatgacatt acgttggatg tcagccgccg tatatacgaa gccgcccgct aagcttttta
    19801 cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac acctcaccgg
    19861 agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac cgttattaag
    19921 ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc aagtaatgag
    19981 gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat tgataccgcc
    20041 gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc agtcaacaga
    20101 gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc ccgcgaagcc
    20161 ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctactt aatgcactgg
    20221 cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga attgcaaaaa
    20281 gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct gcaacgcctg
    20341 attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcatcc gctgatgcaa
    20401 caacgccagc tacacgcctg gaacgcgaca cacaaaatcc agaccgaatc ctggagccca
    20461 ttagcgcaag gagggaaagg cgttttcgat cagaaagtca ttcgcgatct ggcagataaa
    20521 tacggcaaag ccccggcgca gattgttatc cgctggcatc tggatagcgg cctggtggtg
    20581 atcccgaaat cggtcacacc ttcacgtatt gccgaaaact ttgatgtctg ggatttccgt
    20641 ctcgacaaag acgaactcgg cgaaattgca aaactcgatc agggcaagcg tctcggtccc
    20701 gatcctgacc agttcggcgg ctaacatgca aattctcccg gtgacggtaa tgttccgcta
    20761 ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgttac tcttccttgt
    20821 tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta aaagaacaac
    20881 tcatctatat ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa taatacttct
    20941 gtttttagca gccctggcaa gttttaccgt acacgcgcag cctccttcac agaccgtaga
    21001 acaaacagtc cggcagattt atcagaacta taaatcagat gccagtacac cttatttcgg
    21061 tgaaaccgga gagcgggcga taacttcagc gcgtattcaa caggcgctta ccctgaacga
    21121 caatcttacg ctgccgggca atattggctg gctggattat gatccggttt gtgattgtca
    21181 ggattttggc gatctggtgc tagaaagcgt tgcgataacc caaactgacg ccgatcatgc
    21241 cgatgccgtt gtgcgctttc gtatctttaa agatgataaa gaaaagacca cgcagacact
    21301 gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca atcatggcag
    21361 cgtcttacaa gcagttaata gcgagaatga aaaaacgctg gccgctttag cttcgttgca
    21421 aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg attatagctg
    21481 gccgtggacg tgggtggttt ccgactctta ccgccaggcg gttaatgcct tctataaaac
    21541 caccttcaag acggccaata atcccgatga agatatgcaa atagaacggc aatttattta
    21601 cgacaatccg atctgttttg gcgaagagtc gctattttca cgcgttgatg aaattcgagt
    21661 cctggagaaa accgccgatt ccgcccgcat tcatgttcgt tttacgctga ccaatggcaa
    21721 caacgaagag caagaactgg ttttacagcg gcgcgaaggc aagtgggaaa tcgctgattt
    21781 tatccgcccg aacagcggca gcctacttaa gcagattgag gcaaaaaccg ccgccagatt
    21841 aaagcaatga gctgaattaa ataacaatta gccggaacaa taaataaaag ggaacactat
    21901 atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct cagtggctgc
    21961 gtcaatgagc aaaaggtcaa tcaactggcg agcaatgtac aaacattaaa tgccaaaatc
    22021 gcccggcttg agcaggatat aaaagcacta cgcccacaaa tctatgctgc caaatccgaa
    22081 gctaacagag ccaatacgcg tcttgatgct caggactatt ttgattgcct gcgctgcttg
    22141 cgtatgtacg cagaatgata aaaaaatccc cggcagcatg tcagttgccg gggatttttt
    22201 ttaacgtcca accgccgctt tagggcgttt cttcgcacca gcattcaccg gacgagattg
    22261 cgtagacgac gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca tcggcgtatg
    22321 tttagtcaac gccggacggg tattgcggtt ctggcgacga gcttcacgca tctcttcaat
    22381 ggttggcgca ggcactaagc aatcgcgacg gctgccaatc agatgctttt tgcccatcgc
    22441 ttccagcgcc gggcggatta acggccagtt tgccggatcg tggtaacgca acaacgcttt
    22501 atgcaaacga cgctgtttgt cgcccttcgg tacgaagacg tcttcgctct tataaccaat
    22561 cttcgccagc ggttttttcc cggtgtaata catggtggtt gagttcgcca gcggcgatgg
    22621 gtagaagttc tgtacctggt cgagacggaa acgatgcttt ttcagccaca gcgccaaatt
    22681 caccatatct tcatcacgcg taccggggtg cgcagagatg aaatacggga tcagatactg
    22741 ctctttacct gcctgtttcg agtaggtatc gaacagctct ttaaagcggt catagctgcc
    22801 catgcccggc ttcatcatct tcgacaacgg cccttcttcg gtatgttccg gagcaatctt
    22861 cagataaccg ccgacgtgat gggtcgccaa ctctttgata tagcgcggat cttccacggc
    22921 gatgtcataa cgtacgcctg aggcgatgag gattttttta atgcctttca gatcacgcgc
    22981 acggcgatag aggttgatcg tcggttcgtg gttagtgtcc atgtgcggac aaatatccgg
    23041 ataaacgcac gacaaacggc gacaagtttg ttcagcgcgt ggcgatttgc agtgcaacat
    23101 atacatgttg gcagttggcc caccgagatc ggaaatcacg cccgtaaaac ctggaacggt
    23161 gtcgcggatc gcttcgatct cattaatgat cgaatcttcc gaacggctct gaataatgcg
    23221 cccttcgtgc tcggtaatag aacagaaaga acagccgcca aagcagccgc gcataatgtt
    23281 gaccgaaaaa cggatcattt cgtaagccgg aatacgggca ttgccatagg ccggatgtgg
    23341 cacgcgcttg tacggcagcg caaaaacgct gtccatctct tcggtagaaa gcgggatagc
    23401 aggcgggttg atccacacat agcggtcacc gtgtttttgc atcaatgcgc ggacgcagcc
    23461 tgggttggtt tcgtggtgca gaatacgcga agcatgggcg tacagcactt tatcgccctt
    23521 cactttctcg aaagaaggca gcaacacgta ggttttttcc cacggtttcg ggcgcggtgg
    23581 ctgcacggtt acggctttgg cttcctgctt tttcggtgcc accggtttgt tatccgcgca
    23641 cggcaaatct tcaccatacg gatgcgggat tgggtcgatt tttccagggg tatcaagacg
    23701 gggggaatcc acgccgctcc agccaggcag cgcctctttc acgataatcg cggtattacg
    23761 cacatcgcgg atttcactaa tcggctcgcc catcgccaga cggtgcgcca cctccaccag
    23821 cggacgctca ccgttaccaa acatcagcat gtcggctttc gaatccacca gcacggaacg
    23881 gcgcacggta tcggaccagt aatcataatg tgcggtacgg cgcagactag cctcaatacc
    23941 gccgaggatc accggtacat ctttccacgc ctctttacaa cgctgggtat aaaccagtgt
    24001 ggcgcgatcc gggcgcttac ccgcgacgtt atccggcgtg taggcatcgt catgacgtaa
    24061 acggcgatcg gcggtataac ggttgatcat cgaatccatg ttgccagcag taacaccgaa
    24121 aaacagattc ggtttaccca gacgcataaa gtcgtctttg ctgctccagt ccggctgggc
    24181 gatgatcccg acgcgaaagc cctgtgcttc cagcatacga ccgcaaatcg ccatcccgaa
    24241 gcttgggtga tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc
    24301 aagttgatcc atctcttcac gagacatcgg caaaaacggt gccggtccaa aacaggcggc
    24361 ccagtactgc ggccaggaga acaggtcgcg atccggttgg atcagggaga tagagctcat
    24421 tttgcttcca gaaatgataa aaaaataatc aaaggccggg gattataagc cggaacgaaa
    24481 gagaaatcga aaagtattcc atactcgccc tcctcgggcg agtatgaaga ttacggtacc
    24541 ggattgacta acagttgccc aatcgagcca cggtccgcca tttccagcgt ctgactgttg
    24601 aagtagaacg ggaagtgcgc ccaggaaggc tgaccgaaat aaacaagcag ctccacttgt
    24661 ccgtcaaccc aaacggtatc tttccagcct ctgtcttccg ggaacggcat cgcaccattc
    24721 acgttacgga tctggaacat cacgccttca atatggaacg cttgcggctc gtccgcgcgt
    24781 accgtccagc gttcccacgt tccttgctgc gcggtgacat caatacggtt gacgtcccac
    24841 agctgtccat taatacccgg gtcatcaccc agactgatat cgcgactgcg aatcggcgaa
    24901 ccggccatga tttcagttgg cagcaagcgc atcggaagac tgtctgtgac cagcggcaga
    24961 aggccggttg ggcgcagcgt tagcaccagg gtagaaacca gaatactgga tggctcaaag
    25021 aagccgcgaa tacgatcaac aatgctcgcc gcttcgccac aggtgatcga cacttcatcg
    25081 ccgttgctca tatccaccag aatctcgcgg cgctcgcccg gtgccagcga aagttgcttc
    25141 accgatacag gagcaggcag gaatccctga tcgccagaaa tcacatgtaa cgggcgacca
    25201 tcgctcatct gtagttgata gcgacgagag ttcgacgcgt tcagcagtcg caagcgcacc
    25261 cagccacgcg agacttcaac gtacgggctt tgtacaccgt taaccagcag cgtatcacca
    25321 acaaagccgc cgcttcccgg ttcgttgtat tctggcgtac caaagttatc cagccgttta
    25381 tcctggataa tgaccggaaa atcatccaca tcataatggt tggggatagg cagcgacttg
    25441 ctgacttcat cttccaccag ccacattccg gcaaggccgt tatagacctg ctgagccgtg
    25501 cggttgggag tattggcgtg ataccacaga gtagctgcgt tctggcgaat aggcagtacg
    25561 ggtgcccagt cagcgtttgg cgacatcatc cgtgccggac cgcccatcag cgggccaggt
    25621 acctgtagcc cggccaccgt cattgagaca ttttctgtca ggcggttgct gtaaataagc
    25681 ttaacatcgt cgcccttcca gacgcggata gtcggcccca ggtaacgacc attgattccc
    25741 cagaccgacg cgcgtgtccc tggcgtaaat gaccagtgcg cacgttgtac agtcataaac
    25801 agcggttgcc cacggcgaga ttcaagtagc ggcggaacgg gtagcggttg ctgttgcccg
    25861 gctgcgctgg ccttcagggg aacagcgcct gcacaaagtg caatccccga tgcctgaatg
    25921 aactgacgcc gactgagtga catatttgct ccatgtaaaa ctggctaaag taataccgtg
    25981 caggaaagtg ttccccgcaa aaatacagat tgccgactta aacttttccg gcggcttcgc
    26041 gttctgcgac ttctttatcg agctcggcga ttttttgttc cattatcgaa cgacaatggg
    26101 cagccagctc acgaacctga tctttgccat actgactgac gtcaattggc ggcagcattt
    26161 cgacaatcac cagaccgttg tgcagtcgat taagattaat cttattcgaa gttgtagaga
    26221 cgcacacggg aataatcggg acgcccgccg caattgccgc gtgaaatgct ccagtcttga
    26281 acggtagcag gccgcgacca cggctgcggg ttccttccgg gaacatccag atggaaatac
    26341 ggcgtttttt gaaatgattc acgacttccg caatggtgcc gtgagcttta gtgcgattgt
    26401 ttctgtcgat caataagttg ccggttaacc agtacaactg cccgaagaag gggatccaca
    26461 gcaagctctt tttacctacc gtcaccgtcg gcggttgcac aatgttcgat gctgtcacca
    26521 tgtcatagtt gttctggtgg ttagcgatat agatagcatt gccgtagctt tcagcatctg
    26581 caggtttgcg gcactcaact ttcaggccga acagcggcgc aagacggcca aacatatgcc
    26641 caaaggtggc cacatgtttc gggttacgcg ggctgaaaag gcagtaaatg gagccgaata
    26701 cacagactaa gatgctgtaa atcacggtaa taataagacg aaagatatat agcataacac
    26761 cctctgaagg tctgagagac tggcattgta cgtcacctga aatcaggtta gggcattgtt
    26821 aaaagccgct ctctggaaaa gcgtattgtt aatcgcaacg catgaataaa caacggtttc
    26881 aagggaattt ttatcgtttc cgggggttga atacgctgcc ggatgacgac ttaacgtttc
    26941 atccggcgtt ccttgcaagc gggaggaaac aggccctccc cggcatatta ctcttcgcta
    27001 tcaccgttgc tggcacggcg aggagagtcg atctcaacac gatcgatacg ctgcaaaccg
    27061 cgcatcaacg taccgcggcg tccacgttca ccagtgactt tctgtaactc ttccgggcgc
    27121 agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg cagaacgtac
    27181 aattgcgcca gaccatcttc tccacgcgcg gcttctgccg atggaatgtt gataatcttg
    27241 ttgcctttgc ccttcgacag ctgcggcaga tcgcttaccg ggaacatcaa catacggcct
    27301 gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg cggcataaca
    27361 tgggcatttt ccggtaaggt gatcaaagcc ttacctgcac ggttacgcgc caccagatcg
    27421 ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt ctgatcgtcg
    27481 ctttccatca gcatatggtc aacggtcgcc ccaggcggca acgttaattt gccggtgagt
    27541 ggttcgccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata gctacgaccg
    27601 gtggaatcaa caaacactac cgattggttg ctcttacctt tcaccgccac tttgaagcta
    27661 tcacccgctt tataattcag gcccggcgcg tcgatatcat ggcctttagc gctgcgtacc
    27721 cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc gtgctcgctc
    27781 atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gatcgtcacc gtaggcttgc
    27841 gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga agccaaaatg
    27901 ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat cttcatctct
    27961 tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg ggtttccgta
    28021 aggccaaacc gcgacatcag cgccggtttc ggttcatctt cgttacgaat gatctcaatc
    28081 acttcgtcga tgttgagaaa cgccaccagc aaaccttcca ggatatgcag gcgcttgagg
    28141 actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcgacggaa caccagccat
    28201 tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc gatcatattg
    28261 aggttaatgc gatagctctt ttccagatcg gtggtagcga agaggtggtt catcacctga
    28321 tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt ctcgtggtca
    28381 gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg cgcagcaatt
    28441 tgctccagta cacgcgcacc tgaaacctga tgcggtaatg cgctgataac caccgcgcca
    28501 tcttctttct tccacaccgc gcgcatacgt actgaaccac gtccgttctc gtagatttta
    28561 cggatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg cccctgcacg
    28621 atatccagca gctgatcgag cgtggttttc ggctggtcga ttaatgcgat tgccgcctga
    28681 gctacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc aataccggtg
    28741 gtgccgttaa gcaaaatgtt tggcagacga gcaggcagca ttttcggctc ctgcaaagtg
    28801 ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca gctcgctcaa tagcagctcg
    28861 gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt cggatcgtcc
    28921 ggcgcgcccc agttcccctg accatcaacc agcggataac ggtaagagaa cggctgcgcc
    28981 atcaggacca tcgcttcata acaggcgata tcgccgtgcg gatggtattt acccagtacg
    29041 tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc cagttcagac
    29101 atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa cggcaacgca
    29161 cggtccatga tcacgtacat ggagtagttt aagtaggcgt tttccgtaaa ttcatgtagc
    29221 gcaaggcgct ctgccatatc gctcattaat tctgattcct caactgattc gccagccttc
    29281 aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac aggagatcag
    29341 cgtgaatagc gccgccggac gtgctccgac ggcgataaag gcatcaatgt gccttgatat
    29401 acaacgcttt gctgtagact tgatcggcat agagggcagc acaaggttgc gcccatgcca
    29461 caccactcgg catgggggtc cgctccgggc gattcgagaa gttgaggtta tgcgagtcca
    29521 gggaagccta aagcatcaaa gtagtcgact gtaagtatat ggttcagccc agtttagctg
    29581 tgtctccacc agtgccccgc gcctcctgcc ataagtgcgg cttgctcatg ggtacccccg
    29641 agacggctta cggcgcaccg gataataaat ttcgcgtata catggtattc cttaccggag
    29701 aaattataag cccaacaaca atacgcctgt ggcattgttc gctcaaactt atcgcgagtg
    29761 atttggctca cactccggta aagattcccc atcacgctaa cggcacataa acatccgttt
    29821 ccagtcgctg ctccgtcacc ccttcggcaa gattggtgta gtgaaacaga atcggtgctt
    29881 tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat aatgccccat atcgtgtgtg
    29941 aaatatcgtc cagctcgcca acgtggcggg ccacggcata acgtccaccg gtaagttcac
    30001 cattgctcac accataacga ttatcgggaa tcggttcgct aacgctgccg cagatatcaa
    30061 agcgaaacgc ttccggtgcg gtggtggcag ggtcgtccca ggcgacgcca aaagtctggc
    30121 tttggttaac aggagaaagc cccgtctctt tacgccacat gataaatttc gccacactgt
    30181 agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc cacagggatt gatgggaaat
    30241 cgataatgtt tacatccagt gtcaggtttg tcattttgcc tccctgcgaa attattcccg
    30301 ggaacgcgtc ccgggagcgg taacaattac ggatttactt tgcggatctg tttgacgtca
    30361 atttcaacag aattccagtc tttatcgact tcaccctgaa tctcaaccgt atctttcggc
    30421 gtcaccgtca caccgttcca gcgtttgtgg tcgatatcaa cattgatagt accgctggca
    30481 tctttaaaca cgtagagatc gtcagagatg cgttcaacga tattgccgcg cagggttacc
    30541 caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg ttacgctgcc gttcggcccc
    30601 tggaatcctc cggcctgact ttgcgttgcc gatgggccag aaaaaccgcc ctgctctgct
    30661 gccatcaccg gtgcactgca cagggccatt actgcgatta ctgctgcgaa ttttttcatg
    30721 tttattactc cctttaatgt ctgtttccga gcatttaaca agatagtcct taacaacttc
    30781 ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg ttacaacacg gtttactggc
    30841 agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt tactgataga agatgacatg
    30901 ctgattggcg acggcatcaa aactggcctt agtaaaatgg gttttagtgt cgactggttt
    30961 acacaaggtc gtcagggaaa agaggcgctt tatagcgcac cttatgatgc ggtgatcctg
    31021 gatttaacct taccaggcat ggatggtcgc gatattttgc gcgaatggcg agagaaaggt
    31081 cagcgtgagc cggtactgat cctgaccgcg cgtgatgcgt tagcggaacg tgtagaaggg
    31141 ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg cgttgataga agtcgccgcc
    31201 aggctggagg ctctgatgcg ccgaaccaac ggccaggcca gcaacgagct gcgccacggt
    31261 aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg ctggcgaacc cttaacactg
    31321 aaaccaaaag aatttgccct gctggaatta ctgatgcgta acgctggtcg ggtactgccg
    31381 cgcaaactga ttgaagagaa actgtatacc tgggacgaag aggtcaccag taatgccgtt
    31441 gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg attttattcg taccgtgcat
    31501 ggtattggct acacattagg tgagaaatga aatttaccca acgtcttagt ctgcgcgtca
    31561 ggctgacgct aatcttttta attctggcct cggtgacctg gctgctttcc agctttgtcg
    31621 cctggaaaca aacaacggat aacgtcgatg aattgttcga cacccaactg atgctgtttg
    31681 ccaagcggtt aagtacgctc gatctcaacg aaatcaacgc ggcggatcgc atggcacaga
    31741 cgccaaataa attaaaacac ggtcatgttg atgacgatgc gctgaccttt gccatcttta
    31801 cccacgacgg cagaatggtc cttaatgatg gcgataacgg agaagatatt ccctatagct
    31861 atcaacggga aggttttgct gacgggcaac tggtcggtga agacgatcct tggcgttttg
    31921 tctggatgac ctcacctgat ggcaaatatc gcatcgttgt tggccaggaa tgggaatacc
    31981 gtgaagacat ggcgctggcg attgttgccg ggcaattgat cccgtggctg gtcgcactgc
    32041 cgattatgtt aatcatcatg atggtactac tgggtcgtga actcgcgccg ctgaacaaac
    32101 tggcgctggc actacgtatg cgtgaccctg actcggaaaa accactaaac gcgactggcg
    32161 tacccagcga agtgcgacca ctggttgagt cgctaaatca actgttcgcc cgcacacatg
    32221 cgatgatggt tcgagaacga cgctttacct ccgatgcagc tcacgaacta cgtagcccgt
    32281 taacagcgct gaaagtgcaa accgaagttg cgcagctctc tgacgatgat ccgcaggcgc
    32341 ggaaaaaagc actgctccaa ttacattccg ggatcgatcg cgctactcgt ctggttgatc
    32401 aactgctcac gctatcgcgg ctggactcac tggataacct tcaggacgtc gcggagatcc
    32461 cgcttgaaga tctcctgcaa tcgtcggtga tggatattta ccacacggcg cagcaggcga
    32521 aaattgacgt gcgactgaca ctcaatgccc acagcatcaa acgcactgga caaccgctat
    32581 tgctaagttt gttggtgcga aaccttctgg ataacgcagt gcgctacagt ccacagggca
    32641 gcgtggtgga cgtcacgctg aatgccgata atttcatcgt gagggataac ggccccggtg
    32701 tgacaccaga ggcactggcg cgaattggcg aacgcttcta tcgccccccc ggacaaaccg
    32761 ctaccggcag tggacttggg ctatcgattg tccagcgaat cgccaaattg catggcatga
    32821 atgttgaatt tgggaatgcg gaacaaggtg gatttgaggc gaaggtgagc tggtaagggt
    32881 aagacttttg ctaaattcgc aaaagacttt gcacattttg ctaattttac cgtaccgctc
    32941 tgtgacgtac tatagtcgac aaacgtctca ccttgaggtt aaaaaatgag caacaccctg
    33001 attatcaacg gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc
    33061 gaagtcgcgg atggcacact gcgcgacctc gggcatgatg ttcgcatcgt tcgcgccgac
    33121 agcgactacg atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg
    33181 cagatgccag gctggtggat gggcgcgccg tggacagtga aaaaatacat tgatgatgta
    33241 ttcaccgaag gtcacgggac gctgtatgcc agcgatggcc gtacccgcaa agatccgtca
    33301 aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat atatgctttc tctgacctgg
    33361 aacgcaccaa tggaagcctt caccgaaaaa gatcaattct tccacggcgt tggcgtcgac
    33421 ggtgtgtatc tgccgttcca taaagcaaac caattccttg gcatggaacc gctgccgaca
    33481 tttatcgcta atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc
    33541 aagcatcttg tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg
    33601 cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg
    33661 gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg
    33721 ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg
    33781 atgattgagc agtgggaaag catcgcgcac cttgaagcgc atctgcaaac cccgcacatg
    33841 aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga tgaatattcg tattctgcag
    33901 ccagggattt aatcctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt
    33961 taaacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag
    34021 cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg
    34081 atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc
    34141 gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt
    34201 aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg
    34261 agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg
    34321 cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc
    34381 gcatccgcga ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgttagga
    34441 tcgataccga tcgctaccga aatatcatgc acttcctgcg aggccagcac ttcgtcggaa
    34501 gaaacttccc aggtgttaag gatcttacct ttcagcggca tgatcgcctg atattcacga
    34561 tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacgaggaa cagttcggta
    34621 cggttaaggt cctgcgcggt acaatcagcc agtttgccag gcagcgccgg gccgctggtc
    34681 agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct ggaaatcgcc
    34741 atctctgcca gcaactccgc cgcctgaacg ttctggttca gccacaggat aaaggcatct
    34801 ttcaccacgc cagaaacgaa tgccgcgcat tgacgcgaag agagacgctc tttcgtctgc
    34861 ccggcaaact gcggatcctg cattttcacc gacagaacat aggcgcagcg atcccagata
    34921 tcttccgccg acagcttcac gccgcgcggc agaatgttgc gatattcaca gaactcacgc
    34981 atcgcgtcca acaggccctg acgcaggcca ttaacatggg taccgccctg catcgttggg
    35041 atcaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc
    35101 cagtccacag cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc
    35161 ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc
    35221 tgttcggtgt tgttgatctc atctttaaag gtaatctcaa cgccggggca cagtaccgct
    35281 ttggctttca gcacatgcgt caggcgtgaa acagaaaatc gcgggctgtc aaagaaggtt
    35341 tcatccggcc agaagtgcac gctggtaccg gtattgcgtt taccgcaagt gccgacaacc
    35401 tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg
    35461 cgcacgttaa cttcaacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc
    35521 aggccgccag agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa
    35581 agaatcaatt caaccgccgg gacaccctct tccgggtgaa tatccaccgg catcccgcgc
    35641 ccatcgtcaa taacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg
    35701 tgacccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg
    35761 cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc
    35821 tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg
    35881 tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt
    35941 gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc
    36001 ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc
    36061 ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata
    36121 ttgctgcccg gtgtatgggt tctcgttctg accgagatag tccgtcagca gttcaaacgg
    36181 gcgcaccgcc gggtttacca ccactgcggg cagcataaaa cattgtgaca accaggtggc
    36241 gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac
    36301 aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat
    36361 ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg
    36421 cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg
    36481 gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca
    36541 gcatgtaact cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa gttggaacag
    36601 tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg
    36661 agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc
    36721 agcacggtat ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca actacaaccc
    36781 gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt
    36841 ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc
    36901 tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt cgccggggag
    36961 atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc
    37021 cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag
    37081 gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctgatgtgga
    37141 cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagagcttcg
    37201 tgcttttgtg caaacaggtg agtgtcggta atttgtaaaa tcctgactct ggcctcacca
    37261 gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg atgctagtaa
    37321 accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactga
    37381 ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct
    37441 ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga
    37501 cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc
    37561 accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc
    37621 tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa
    37681 aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat
    37741 gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac
    37801 gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca
    37861 tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg
    37921 tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa
    37981 cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg
    38041 gcaacatctt ccacactttc accctcttct atcatcccgg caaccatctc cagtagccag
    38101 ggggtttcac tggtgtcgta tgcggcaatc cgaatctgct caatcagcac aacttcatcg
    38161 cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttcccgccgt
    38221 acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag atctaatgaa
    38281 aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta
    38341 acgggcaggt tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg
    38401 tgaatttcag cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta
    38461 acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat
    38521 aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat
    38581 tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga
    38641 acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg
    38701 ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc
    38761 taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact
    38821 ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc
    38881 gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc
    38941 agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg
    39001 ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc
    39061 aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc gcgcagtacg
    39121 ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc
    39181 tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc gaaaacttta
    39241 aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt
    39301 cgctgttaca ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg
    39361 atggtcactt accgacgctg gatttaacgg cttctaccgg gatttctgac acctcttata
    39421 gcggttcgaa aacccgtggt gccgctggta cccagtatga cgacagcaat atgggccaga
    39481 acaaagtggg cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg
    39541 tgaaacaggc ccagtacaac tttgttggtg ccagcgagca actggaaagc gcgcatcgta
    39601 gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcatctatc agtagcatta
    39661 acgcctacaa acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct
    39721 actcggtcgg tacgcgtacc attgttgatg tgttggatgc aaccaccacg ctgtacaacg
    39781 ctaagcaaga gctggcaaat gcgcgttata actacctgat taaccagttg aatattaagt
    39841 cagctctggg tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac
    39901 cggtttccac taatccggaa aacgttgcac cgcaaacgcc ggaacagaat gctattgctg
    39961 atggttatgc gcctgatagc ccggcaccag tcgttcagca aacatccgca cgcactacca
    40021 ccagtaacgg tcataaccct ttccgtaact gacgacgacg acggggcttc ggccccgtct
    40081 gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca
    40141 atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa
    40201 tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc gcacgccatc
    40261 tgacaccagt cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg
    40321 atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa
    40381 gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga
    40441 aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac
    40501 cagcccaggc tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt
    40561 tctggatgcc gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat
    40621 ttgcacagca gccgctgttc tcctcgaaaa acccagccag cccggcttac ggtaaatata
    40681 ccgacgcgac gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga
    40741 agacagccat ggcaccaaaa ccggcgacca ctactaccgt tacccgtggc ggttttggtg
    40801 aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct tctcgttcaa
    40861 tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgcgaaaa
    40921 agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga
    40981 tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact
    41041 gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa
    41101 attccgcatt ccaaaacgca cctggagttt tgtgcgtcag tcatggctga ctcaccagcc
    41161 atcgctttat tcgcgtcttg atctggcatg ggatggcact ggcgaaccta aactgctgga
    41221 aaataacgcc gatacaccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct
    41281 ggaagatcag cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca
    41341 agaaaagcta atcgatcgct ttgtcgagtt gcgtgaacag tatggcttcc agttgctgca
    41401 tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg
    41461 cgcgacggaa gctgaaatcg ctaccgagtt cctctacatt gacgatatcg ggttaggtga
    41521 aaaaggtcag ttcacggatt tacaggatca ggtaatttcc aacctgttca agctgtatcc
    41581 atgggaattt atgttgcgtg agatgttctc caccaagctg gaggatgcag gcgtacgctg
    41641 gctggaaccg gcgtggaaga gcattatctc caacaaagcg cttctaccgc tactatggga
    41701 gatgttcccg aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca
    41761 aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat
    41821 tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt
    41881 tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct
    41941 ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcgttga tcacccagga
    42001 tatgtctcgg ttttatccac atatttttat tgagtaatca atgatgccgg atggcattcg
    42061 ccatccggta attgttaacc tatctgcacc gacagcatac tcaggctgcc catttctata
    42121 ccatcgaccg gaatggtaat tggctcctgc ccatcccacg cacctaagac atacaacaac
    42181 ggcagatagt gctccggcgt tgggttcgat aacgcgccac cttcatggtc aaggtaattc
    42241 accagaggat gctgctctac cgccccttgc cacgtcagat tcgctttcac atactcatta
    42301 aacgacatcg cccaaggata tggtgaacta tcaccgtgcc acttcactgt acgcaggtta
    42361 tgcaccacgt tgccactggc gaccaacatt attccttcat ctcgcagcgc tgccagtttg
    42421 cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc
    42481 gggatatcgg cgtcaggata catcttaatc agcacgcccc aggaaccgtg gtcaaagccc
    42541 caggcttctt tatccagcgt caccgggacc gacgctaaca gctcaaccag atgctgtgcc
    42601 agcacaggcg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca
    42661 aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag
    42721 tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag
    42781 ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat
    42841 gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc
    42901 tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt
    42961 attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc
    43021 attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg
    43081 caaaaaccct cgaaccgctt actggcgttt tcgctaggat ttgcggcggg gatcatgttg
    43141 ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg
    43201 ttgggttatg gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg
    43261 ccacatgcgc atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc
    43321 aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt
    43381 gccacctttg tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc
    43441 gcgttgcaca atatccctga aggtctggca gtggcaggcc cggtttatgc ggcaacgggg
    43501 tctaaacgca ccgcgattct gtgggcgggg atttctggac tagcagaaat tcttggtggt
    43561 gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc ggcaatcatg
    43621 gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa
    43681 gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga
    43741 ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca gaagttgtcc
    43801 agcacatgga caacttctgc cagcttcacg aaagccttat tctacctttt cggctgtctc
    43861 ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg
    43921 aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca gagcaatcaa
    43981 tacaatccag taccaggcga taa
//
LOCUS       DAZSBF010000004        43713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZSBF010000004 DAZSBF010000000
VERSION     DAZSBF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.004"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHXWD_000138"
     CDS             complement(<1..559)
                     /locus_tag="ACHXWD_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR7619177.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHXWD_000139"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHXWD_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR7619178.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHXWD_000140"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHXWD_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR7619179.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHXWD_000141"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHXWD_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR7619180.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHXWD_000142"
     CDS             complement(4001..6322)
                     /locus_tag="ACHXWD_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR7619181.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHXWD_000143"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHXWD_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR7619182.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHXWD_000144"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHXWD_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR7619183.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHXWD_000145"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHXWD_000145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR7619184.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHXWD_000146"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHXWD_000146"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR7619185.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHXWD_000147"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHXWD_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHXWD_000148"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHXWD_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR7619186.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHXWD_000149"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHXWD_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR7619187.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHXWD_000150"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHXWD_000150"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR7619188.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHXWD_000151"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHXWD_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR7619189.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHXWD_000152"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHXWD_000152"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR7619190.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHXWD_000153"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHXWD_000153"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR7619191.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHXWD_000154"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHXWD_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR7619192.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHXWD_000155"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHXWD_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHXWD_000156"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHXWD_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR7619193.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHXWD_000157"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHXWD_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR7619194.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHXWD_000158"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHXWD_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR7619195.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHXWD_000159"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHXWD_000159"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR7619196.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHXWD_000160"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHXWD_000160"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR7619197.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHXWD_000161"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHXWD_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR7619198.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHXWD_000162"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHXWD_000162"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR7619199.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHXWD_000163"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHXWD_000163"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR7619200.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHXWD_000164"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHXWD_000164"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR7619201.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHXWD_000165"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHXWD_000165"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR7619202.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHXWD_000166"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHXWD_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR7619203.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHXWD_000167"
     CDS             32202..32564
                     /locus_tag="ACHXWD_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR7619204.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHXWD_000168"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHXWD_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHXWD_000169"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHXWD_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHXWD_000170"
     CDS             35034..35912
                     /locus_tag="ACHXWD_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR7619205.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHXWD_000171"
     CDS             35923..36276
                     /locus_tag="ACHXWD_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR7619206.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHXWD_000172"
     CDS             36288..37592
                     /locus_tag="ACHXWD_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR7619207.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHXWD_000173"
     CDS             37604..38689
                     /locus_tag="ACHXWD_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR7619208.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHXWD_000174"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHXWD_000174"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR7619209.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHXWD_000175"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHXWD_000175"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR7619210.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHXWD_000176"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHXWD_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHXWD_000177"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHXWD_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR7619211.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTEC
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="ACHXWD_000178"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="ACHXWD_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR7619212.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGELIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11126 c        11988 g        10442 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggtagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cattcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaactcg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DAZSBF010000005        43228 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZSBF010000005 DAZSBF010000000
VERSION     DAZSBF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.005"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            12..530
                     /locus_tag="ACHXWD_000179"
     CDS             12..530
                     /locus_tag="ACHXWD_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR7619213.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            546..1085
                     /locus_tag="ACHXWD_000180"
     CDS             546..1085
                     /locus_tag="ACHXWD_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR7619214.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="ACHXWD_000181"
     CDS             complement(1180..2757)
                     /gene="guaA"
                     /locus_tag="ACHXWD_000181"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR7619215.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="ACHXWD_000182"
     CDS             complement(2826..4292)
                     /gene="guaB"
                     /locus_tag="ACHXWD_000182"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR7619216.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4454..5824
                     /gene="xseA"
                     /locus_tag="ACHXWD_000183"
     CDS             4454..5824
                     /gene="xseA"
                     /locus_tag="ACHXWD_000183"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR7619217.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5821..6036)
                     /locus_tag="ACHXWD_000184"
     CDS             complement(5821..6036)
                     /locus_tag="ACHXWD_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR7619218.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6105..7577)
                     /gene="der"
                     /locus_tag="ACHXWD_000185"
     CDS             complement(6105..7577)
                     /gene="der"
                     /locus_tag="ACHXWD_000185"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR7619219.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7695..8873)
                     /gene="bamB"
                     /locus_tag="ACHXWD_000186"
     CDS             complement(7695..8873)
                     /gene="bamB"
                     /locus_tag="ACHXWD_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR7619220.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8884..9504)
                     /gene="yfgM"
                     /locus_tag="ACHXWD_000187"
     CDS             complement(8884..9504)
                     /gene="yfgM"
                     /locus_tag="ACHXWD_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR7619221.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9522..10796)
                     /gene="hisS"
                     /locus_tag="ACHXWD_000188"
     CDS             complement(9522..10796)
                     /gene="hisS"
                     /locus_tag="ACHXWD_000188"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR7619222.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10907..12025)
                     /gene="ispG"
                     /locus_tag="ACHXWD_000189"
                     /gene_synonym="gcpE"
     CDS             complement(10907..12025)
                     /gene="ispG"
                     /locus_tag="ACHXWD_000189"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR7619223.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12052..13065)
                     /gene="rodZ"
                     /locus_tag="ACHXWD_000190"
     CDS             complement(12052..13065)
                     /gene="rodZ"
                     /locus_tag="ACHXWD_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR7619224.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13350..14504)
                     /locus_tag="ACHXWD_000191"
     CDS             complement(13350..14504)
                     /locus_tag="ACHXWD_000191"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR7619225.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14654..15085)
                     /gene="ndk"
                     /locus_tag="ACHXWD_000192"
     CDS             complement(14654..15085)
                     /gene="ndk"
                     /locus_tag="ACHXWD_000192"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR7619226.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15234..17546)
                     /gene="pbpC"
                     /locus_tag="ACHXWD_000193"
     CDS             complement(15234..17546)
                     /gene="pbpC"
                     /locus_tag="ACHXWD_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR7619227.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17547..22508)
                     /gene="yfhM"
                     /locus_tag="ACHXWD_000194"
     CDS             complement(17547..22508)
                     /gene="yfhM"
                     /locus_tag="ACHXWD_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR7619228.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNCLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22715..23560
                     /gene="sseA"
                     /locus_tag="ACHXWD_000195"
     CDS             22715..23560
                     /gene="sseA"
                     /locus_tag="ACHXWD_000195"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR7619229.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23896..24021
                     /gene="timP"
                     /locus_tag="ACHXWD_000196"
     CDS             23896..24021
                     /gene="timP"
                     /locus_tag="ACHXWD_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR7619230.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24053..24829)
                     /gene="sseB"
                     /locus_tag="ACHXWD_000197"
     CDS             complement(24053..24829)
                     /gene="sseB"
                     /locus_tag="ACHXWD_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR7619231.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(24971..26254)
                     /gene="pepB"
                     /locus_tag="ACHXWD_000198"
     CDS             complement(24971..26254)
                     /gene="pepB"
                     /locus_tag="ACHXWD_000198"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR7619232.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26432..26632)
                     /gene="iscX"
                     /locus_tag="ACHXWD_000199"
     CDS             complement(26432..26632)
                     /gene="iscX"
                     /locus_tag="ACHXWD_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR7619233.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26644..26979)
                     /gene="fdx"
                     /locus_tag="ACHXWD_000200"
     CDS             complement(26644..26979)
                     /gene="fdx"
                     /locus_tag="ACHXWD_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR7619234.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26981..28831)
                     /gene="hscA"
                     /locus_tag="ACHXWD_000201"
     CDS             complement(26981..28831)
                     /gene="hscA"
                     /locus_tag="ACHXWD_000201"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR7619235.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28848..29363)
                     /gene="hscB"
                     /locus_tag="ACHXWD_000202"
     CDS             complement(28848..29363)
                     /gene="hscB"
                     /locus_tag="ACHXWD_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR7619236.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29459..29782)
                     /gene="iscA"
                     /locus_tag="ACHXWD_000203"
     CDS             complement(29459..29782)
                     /gene="iscA"
                     /locus_tag="ACHXWD_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR7619237.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29799..30185)
                     /gene="iscU"
                     /locus_tag="ACHXWD_000204"
     CDS             complement(29799..30185)
                     /gene="iscU"
                     /locus_tag="ACHXWD_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR7619238.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30213..31427)
                     /gene="iscS"
                     /locus_tag="ACHXWD_000205"
     CDS             complement(30213..31427)
                     /gene="iscS"
                     /locus_tag="ACHXWD_000205"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR7619239.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31668..32156)
                     /gene="iscR"
                     /locus_tag="ACHXWD_000206"
     CDS             complement(31668..32156)
                     /gene="iscR"
                     /locus_tag="ACHXWD_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR7619240.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32457..33194)
                     /gene="trmJ"
                     /locus_tag="ACHXWD_000207"
     CDS             complement(32457..33194)
                     /gene="trmJ"
                     /locus_tag="ACHXWD_000207"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR7619241.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33313..34116
                     /gene="suhB"
                     /locus_tag="ACHXWD_000208"
     CDS             33313..34116
                     /gene="suhB"
                     /locus_tag="ACHXWD_000208"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR7619242.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34261..35115
                     /locus_tag="ACHXWD_000209"
     CDS             34261..35115
                     /locus_tag="ACHXWD_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7619243.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35306..36586
                     /gene="csiE"
                     /locus_tag="ACHXWD_000210"
     CDS             35306..36586
                     /gene="csiE"
                     /locus_tag="ACHXWD_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR7619244.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36578..37717)
                     /locus_tag="ACHXWD_000211"
     CDS             complement(36578..37717)
                     /locus_tag="ACHXWD_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR7619245.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37877..38767)
                     /gene="hcaR"
                     /locus_tag="ACHXWD_000212"
     CDS             complement(37877..38767)
                     /gene="hcaR"
                     /locus_tag="ACHXWD_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR7619246.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38903..40264
                     /gene="hcaE"
                     /locus_tag="ACHXWD_000213"
     CDS             38903..40264
                     /gene="hcaE"
                     /locus_tag="ACHXWD_000213"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR7619247.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40261..40779
                     /gene="hcaF"
                     /locus_tag="ACHXWD_000214"
     CDS             40261..40779
                     /gene="hcaF"
                     /locus_tag="ACHXWD_000214"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR7619248.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40779..41099
                     /locus_tag="ACHXWD_000215"
     CDS             40779..41099
                     /locus_tag="ACHXWD_000215"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR7619249.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41096..41908
                     /gene="hcaB"
                     /locus_tag="ACHXWD_000216"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41096..41908
                     /gene="hcaB"
                     /locus_tag="ACHXWD_000216"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR7619250.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41918..43120
                     /gene="hcaD"
                     /locus_tag="ACHXWD_000217"
     CDS             41918..43120
                     /gene="hcaD"
                     /locus_tag="ACHXWD_000217"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR7619251.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10257 a        11944 c        10853 g        10174 t
ORIGIN      
        1 aacaaggaat agtgatgaaa tttaaaaaat gtcttctgcc tgtggcaatg ttagcgtcat
       61 tcactctggc aggatgccag tcaaatgctg acgatcatgc cgccgatgtt tatcaaaccg
      121 atcaactgaa taccaaacaa gaaactaaaa ccgttaatat tatttccatt cttcccgcaa
      181 aagttgccgt agacaactcc caaaataaac ggaacgcaca agccttcggc gcgcttattg
      241 gcgcagtcgc tggcggtgtt atcggccaca acgtcggttc tggcagcaat tccggaacga
      301 cggcaggggc agttggcggc ggagctgtag gcgcggcagc gggttctatg gtgaatgata
      361 aaaccttagt ggaaggtgtt tctttaacat ataaggaagg caccaaagtg tatacctcca
      421 cccaggtggg taaagagtgc cagtttacga caggtttagc cgttgttatt accacaacgt
      481 ataacgaaac gcgtattcag ccaaatacta aatgtcctga aaagagctaa taatcaggag
      541 gagtcatgaa gaaagttttt ctttgcgcca tcttagcctc cttaagctat ccggctatcg
      601 cctcatcatt gcaggatcaa ctctctgctg tcgcagaagc ggaacagcaa ggtaaaaatg
      661 aagagcaaag gcagcatgac gaatgggtcg cggagcgcaa cagggaaatc cagcaagaga
      721 agcaacgtcg cgcaaatgcc caggccgccg ctaacaaaag agcggcaacg gcagcggcaa
      781 ataagaaagc tcgtcaggat aaactggacg ccgaagccac tgcggacaaa aaacgcgatc
      841 aaagttatga agatgagcta cgcagcttag agattcagaa acaaaaactg acgctggcga
      901 aagaagaagc ccgcgttaag cgagaaaacg aatttatcga tcaggaactg aagcacaaag
      961 ctgcgcaaac cgatgtggtg caatctgaag ctgacgccaa cagaaatatg actgaaggcg
     1021 gtcgcgatct gatgaaaagc gtgggcaaag cagaagagaa caaatcggac agctggttta
     1081 attaatcgat gttagtaact tcaagcctat gattcttgaa gataaaaaac cctctgtagt
     1141 aacagagggt tttgttcatt catagtgcag ggattaaaat cattcccact caatggtagc
     1201 tggcggcttg ccgctgatgt catacaccac gcgggaaata ccgttcactt cattgataat
     1261 gcggttggaa acgcgaccga ggaaatcgta cggcagacgc gcccagtgtg cggtcataaa
     1321 gtcgatggtt tcgacagcac gcagagagac aacccagtca tacttacgac catcgcccat
     1381 tacgccaacg gaacgtaccg gcaggaacac agtgaacgcc tggctgactt tgtcgtacag
     1441 gtccgcttta cgcagttctt caatgaagat ggcgtcagca cggcgcagca ggtcacagta
     1501 ctctttcttc acttcaccca gcacacgaac gccaaggcct ggtcccggga atgggtgacg
     1561 gtacaacatg tcgtacggca ggcccagctc cagaccaatc ttacgcactt cgtctttgaa
     1621 cagctctttc agcggttcaa ccaggcccat cttcatctct ttcggcaggc cgcccacgtt
     1681 gtggtgagat ttgatgacgt gtgctttacc ggttgcagac gccgcagatt cgataacgtc
     1741 agggtagatg gtgccctgcg ccagccactt cacgtcttcc agtttcagcg cttcttcatc
     1801 gaatacttca acgaaaacgc gaccgatgat tttacgtttt gcttccggat cgttttcgcc
     1861 agccagcgct gacaggaagc gatcttctgc cggaacgtga acaatgttca gaccaaagtg
     1921 atcgccaaac atatccagaa cctgctctgc ttcgttgaga cgcagcaggc cgttgtcgac
     1981 gaatacgcaa gtcaggtttt taccgatagc gcggtgcagt agcattgcgg ttacggagga
     2041 atccacgcca ccagagaggc cgaggatgac tttatcgtcg cctacctgct cacggatgcg
     2101 ggctacagca tcgtcgataa ttttcgctgg cgtccacagg gcttcacact ggcagatatc
     2161 acgcacaaaa cgctccagca tgcgcatacc ctggcgggtg tgagtcactt ccgggtggaa
     2221 ctgtacgcca tagaagcgtt tttcttcgtt agccataatg gcaaacgggc agctttcggt
     2281 gctggctacg gtgacgaagt cggacgggat agcggtaact ttatcgccgt ggctcatcca
     2341 gacatcgagc agcggtttac cgtctgcggt cagcgcatct tcgataccgc gaaccagtgc
     2401 actgtcgttt acgacttcaa cctgcgcgta gccaaattca cgttcgttag aggcttcaac
     2461 gtgaccgccc aactgcattg ccatggtctg catgccatag caaacgccga ataccggtac
     2521 gcctgcttca aagacatact gcggcgcacg cgggctgttt tcttcagtag tactttccgg
     2581 gccgccggaa agaataatgc cgcttggatt gaagtcacga atttgtgctt ctgtcacatc
     2641 ccacgcccac agttcgcagt aaacacccag ctcacgcacg cggcgcgcaa ccagttgagt
     2701 gtactgagaa ccgaagtcta gaatgaggat gcgatgctta tgaatgtttt ccgtcattga
     2761 cgcttattcc gaggcaagtg aaacagataa tataaatcgc ccgacatgaa gtcgggcgaa
     2821 gagaatcagg agcccagacg gtagttcggg gactctttag taatggtcac gtcgtgaacg
     2881 tggctttcct gaatgcccgc accgctgata cgtacaaact ccgctttagt acgcagttcg
     2941 tcgatagtac cacagccggt cagacccata caggagcgca ggccgcccat ctgctggtga
     3001 atgatctctt tcaggcgacc tttataagct acgcgacctt cgataccttc cggcaccagt
     3061 ttgtcggcag cgttatcgct ctggaaataa cggtcagagg aacctttgga catcgcgccc
     3121 agggaaccca taccacggta agatttgtaa gaacggccct ggtagagttc gatttcaccc
     3181 ggagattctt cagtacccgc cagcatggaa cctaccatca ccgcgcttgc gccagcggcg
     3241 atagctttgg cgatgtcgcc ggagaagcga ataccaccat ctgcgataac cggaataccg
     3301 gtgccttcca gggcttcaac tgcgtcagca acggcagtga tctgcggaac acctacgcca
     3361 gtaacgatac gagtcgtaca gatagaacca gggccgatac cgactttaac cgcgctgcaa
     3421 ccagcttctg ccaaagcgcg tgcacctgca gctgttgcca cgttgccgcc gatgatttgc
     3481 agatccggat atttagcacg ggtttcacgg atacgctgca aaacgccttc tgagtgaccg
     3541 tgggaggagt cgatcagcag aacgtcaacg ccagcggcaa ccagcgcgtc aacacgctct
     3601 tcgttacccg cacctgcgcc aactgcagca ccaacacgca gacggccttg ctcgtcttta
     3661 caggcgttcg gtttacgttc cgctttctgg aagtctttca cggtgatcat gccgatcagg
     3721 tggaattcgt catcaaccac cagcgctttt tcaacgcgtt tttcgtgcat ttttgccagc
     3781 accacttcac gggcttcacc ttcacgcacg gtgaccagac gctctttcgg cgtcatgtaa
     3841 acgctaaccg gctggttcag gtcggtaaca aaacgcacgt cacgaccggt gataataccc
     3901 accagttcgt tttcttcggt aacgaccgga tagcccgcaa aaccgttacg ctcggtcagt
     3961 tctttcactt cgcgcagcgt cgtggttggc agaacagtct gcggatcagt caccacacca
     4021 gattcgtgtt ttttcacacg gcgaacttct tctgcctggc gttcaatgga catgtttttg
     4081 tggataaagc cgataccgcc ttcctgagcc agagcaatag ccaggcgcgc ttccgttacg
     4141 gtatccattg ctgcggaaag catagggata ttcagacgaa tagttttcgt cagctgggtg
     4201 ctgaggtcag cagtattcgg cagaacggta gaatgagcag gaacgaggag aacgtcgtca
     4261 aacgtcagag cttctttagc gatacgtagc atgggcaata tctcgaccag agtggttaat
     4321 aaatattgcc gcggcattat acagagcgta accgattgca tctacccctt tttgcaaaaa
     4381 atgcttgcta tccccgaagg gcgggttact atcgactgaa taacctgctg atttagaatt
     4441 tgatctcgct cacatgttac cttctcaatc ccctgcaatt tttaccgtta gtcgcctgaa
     4501 tcaaacggtt cgtctgctgc ttgagcatga gatgggacag gtttggatca gcggcgaaat
     4561 ctctaatttc acacaaccgg cttccggtca ctggtacttt acactcaaag acgacaccgc
     4621 ccaggtacgc tgcgcgatgt tccgcaacag caaccgccgg gtgaccttcc gtccacagca
     4681 tggacaacaa gttttagttc gcgccaatat tacgctctac gagccgcgcg gtgactacca
     4741 gataatcgtt gagagtatgc agccggccgg tgaagggctg ctgcaacaga agtacgaaca
     4801 gctcaaagcg aagttgcagg ctgaaggttt gttcgatcag caatacaaaa aaccacttcc
     4861 ctcccctgcg cattgcgttg gtgtgatcac ctcaaaaacc ggtgctgcgc tacatgatat
     4921 tttgcatgtg ttaaaacgtc gcgatccttc tctaccggtg atcatctacc ccaccgccgt
     4981 tcagggcgat gacgcaccgg ggcaaattgt tcgcgccatt gaactggcga atcagcgcaa
     5041 tgagtgcgac gtgttgatcg ttgggcgcgg cggcggttcg ctggaagatt tatggagttt
     5101 taacgacgaa cgcgtagcgc gggcgatttt tgccagccgc attccggtcg tcagcgccgt
     5161 cgggcatgag acggatgtga ccattgccga ttttgttgcc gatctgcgtg cgccaacgcc
     5221 gtctgccgcc gctgaagtag tgagccgtaa tcagcaagag ttactgcgcc aggtgcaatc
     5281 gacccgtcaa cggctggaga tggcgatgga ttattatctc gccaaccgca cgcgtcgctt
     5341 tacgcagatc catcaccgat tacagcaaca gcatccgcag ctccggctgg cacgccagca
     5401 aaccatgctt gagcgcctgc aaaagcggat gagctttgcg ctggaaaatc aacttaagcg
     5461 taccgggcaa cagcagcagc ggttaacaca gcggctgaat cagcaaaatc cacagccgaa
     5521 gattcatcgc gcgcaaacgc gcattcagca actggaatat cgtttagcag aaaccctgcg
     5581 cgtacagctt agcgccacgc gtgaacgttt cggtaatgca gtaacgcacc tcgaagccgt
     5641 aagcccactg tcaacgctgg cgcgtggata cagcgttact actgctactg acggcaatgt
     5701 actgaaaaaa gtgaagcaag ttaaagcggg tgaaatgcta accacacgtc tggaagacgg
     5761 ctggatagaa agtgaagtta aaaacatcca gccagtaaaa aaatcgcgta aaaaggtgca
     5821 ttaagccagc acgaattcaa cgcgtttttt cgatatcaag ccatgcccgt gctgacagaa
     5881 gtaatctact gcaccgcagg cttttaacac ctgcaacggt tgatggcagt ctggacaaag
     5941 agctttcatt tcaataattt ccccgcagga tgggcaacgc gcatggccgt tatcctgatc
     6001 aagcacatgc tgacattgtg gacaatgcag ttccatatga attcctccgg tagccatccc
     6061 tctacattca tagagggaat ggcagataaa atacttacgg ataattattt atttttcttg
     6121 atgtgcttca tcagacgctt acgtttacgc atctgggttg gcgtcagggt gttacgcttg
     6181 ttcgcatacg ggttttcccc ttctttgaac tgaatacgaa tcggcgatcc cattacgtcc
     6241 agcgatttgc ggaagtagtt catcaagtag cgcttgtagg aatcaggcag gtctttcacc
     6301 tgattaccgt gaatcaccac aatcggcggg ttataaccac cagcgtgggc atatttcagc
     6361 ttcacacgac gaccgcgtac cagcggcggt tggtgatctt caacagccat cgtcatgatg
     6421 cgcgtcagca tagaggtccc cacacgacgg gtggagctgt catacgcttc acgtactgat
     6481 tcaaacaagt taccaacacc actgccgtgc aaggcagaga taaagtgcac acgagcaaaa
     6541 tcgataaagc ccagacggaa gtccagcgtt tctttcacct gctctttcac ttcctggctc
     6601 aggccatccc acttattcac cacaatgaca agtgagcgcc cactattgag aataaagccc
     6661 agcagcgaga gatcctgatc ggaaatacct tcgcgcgcat caatcactaa catcaccacg
     6721 ttggtatctt caattgcctg caacgttttg attacggaga atttctctac agcatcggtg
     6781 attttgccgc gtttacgtac gccagcggtg tcaatgagca catactcacg tccatcgcgt
     6841 tccattggaa tgtagatact gtcacgcgtc gtgccaggca tgtcataaac aacaacgcgc
     6901 tcttcaccaa gaatacggtt agtgagtgta gacttaccta cgttcggacg acccacaatc
     6961 gccagtttga tcggcagact ttgcgggtcg aagtcgtctt cctcttcttc ttcgccgttc
     7021 tcttccgctt caaattgcgc ccagtattca gcgtcttcgt cgacttcctc ttgcggtgcg
     7081 agatcttcca tccacggcag cagcacacgc tccagcagac ttaatacgcc acgaccgtga
     7141 gatgcggcga tcgggtagat ttcacctaaa ccaagcgagt agaaatcaac cactgcctga
     7201 tcgggatcca gaccgtcagt tttgtttgcc accaggaagg tcggtttttc acgagagcgc
     7261 agatgtttgg caatcgcttc atctgccggc atcaagcccg cgcgcgcatc caccataaac
     7321 agtacgacgt ccgcttcttc aatcgccagc agcgactgtt ccgccatgcg ggtttctaca
     7381 ccgtcttctg tgccatcaat cccgccggta tcaatacaga taaactcacg gccttcaatt
     7441 tccgcacgac cgtacttacg gtcacgagtc agacccggga aatccgcaac cagcgcatct
     7501 cgggtgcgag ttagacggtt aaataacgtg gattttccta cgttagggcg cccgacaagc
     7561 gcgaccacag gtaccatgtt taaagcctca tttttataaa tcatcagaca acgcacgcta
     7621 tattcgcgtc gttgttaaaa acaggaaaac ggcccctgtc caggagccgt tttcaaagtg
     7681 aacgacagag acgattaacg tgtaatagag tacacggttc cgtcttttgc ctggatcagc
     7741 agtttgccgt cagcggcaac cggttcagtc tggaaaccgg aactatcaac tttttgctgg
     7801 gcaacgaaac gaccatcttc gacgttaatc cagtgcagat aaccttcact gtcacctacc
     7861 accaggttgc cattatacag caccggcgaa gtcagcaggc gatgcagcag atcgctttgt
     7921 gtccacagcg taacgccgcc atcaatggtc aacgccatca cccggtcatt ttgatcgacc
     7981 agatagatgc gattgccgtc gacgatgaaa tcattcaccg aacccagttc gcgtttccac
     8041 ataatctgac cactgcgcag atcaagcgcc gtcaggttac cattataggc cagagcgaaa
     8101 acaacgccgt taacaacgac gggagtcgtg tcaacatcgc tcagacggtc aatttcggta
     8161 gaaccggtcg cctgggaaat acgctgctgc caaatcatct ggccctgttc catcagcact
     8221 gcgctgacgc gaccattatc gccccccacg acggccgcac caaaagccgt tgccggcgca
     8281 gactcgccac gcaaagagag cgaaggcata tcgaggttaa ctgtccattt gacagcgccg
     8341 tcagcttcgt tcagcgcttg taactgaccg ttactagtgt ggattaacac cagaccgtcg
     8401 ctgaccaccg ggcgagaaag tgcttcaccc gcgactttag tttgccatgc cacagtacca
     8461 tcgctggtat tcagcgcgta aacctgcgcc ttttcgctgc caatgtagac atgcccacca
     8521 gacacggtca caccgccaga aagtaatgca ggctctttag agaaccagcc atctttctcg
     8581 gccaggctga cagaccagat ttctttgcca tcatccgcat tcagcgcttt taccaaacca
     8641 gcgcggtccg ctgcatagac aacgttgtcc gccagtgccg gatgaagatt ggaatagaag
     8701 ttgccaatgc cgctaccaac ggaagtgctc cacgccgtgg tcggcgtaaa ctggttttca
     8761 acggttggca atggggacat ctttaccaca tcttcttcgc tgttaaacag cgaacagccg
     8821 cttaaaaggg taacggaaag cagtcctggc agcagtaatt tacgcaattg catcgggtcc
     8881 ctctcagatg gacaaattat taattttcat ctgcatcatt tcgctcagtg ccggagtaac
     8941 atcgcttttc acgcctgctt cccatgcact acgcgcacct tgcttatcac ctttgctcaa
     9001 caatgcttta ccacgcaggt cggcaacaat ggcagcccac ccttcacctt tgatggtatc
     9061 aagggttttc agcgcggcat cagcctgctt gagctgtacc tgaacgcgag caagacgcag
     9121 atttatcacg gctttgagat tttcatcact cgtgtctgcc agcccctgtt gtaactgggc
     9181 ggcagctttc tctagttcat ttttgtcaac aaattgctgc gcaagttcca aagaagccag
     9241 cgcaccataa gtatttttat tttcagcagc aaatttttcc gccgccggga tgctatccgg
     9301 tttgccttcg ctcactgcgg taaccgcatt ttgataggca agagaagcgg agcgtgcaga
     9361 atcaacctga tggctgttcc agtagcgcca gccaatcagt gcgccaacgc ccaaaatcac
     9421 cccaacagcc agtgctttgc cattttcagc aaaaaagcgt ttaaccgctt ctacctggtc
     9481 gttttcgttc tcgtaaattt ccacgctgtc cttctccttc cttaacccag taacgtgcgc
     9541 aaatgcgcgg ctacgctatc ctgcgcaact gccgtttgct caccagagcg caaatccttc
     9601 actactgctg tgccgttagc cacttcagac tcacccagca ccacagcaac gcgggcaccc
     9661 catttatcag cacgggcaaa ctgtttctta aagttgccgc cgccgtggtt ggtcatcaat
     9721 ttcacgcccg gtaattcatc acgcagacgc tcagctaatg ccatagccgc agattgtgta
     9781 tcagcacctg aagccaccag gtatatatcg acaacaggat cggctttaaa ttccggatta
     9841 acggcctgta ctaacaatac aagacgttcg aggcccatcg caaaaccgac tgccggtgtt
     9901 gcacgaccgc ccagttgttc caccagaccg tcataacgac cgcctgcaca cacggtgccc
     9961 tgggagccga ggctgttagt cacccactcg aaaacggtac ggttatagta atccagacca
    10021 cgcaccagac gctggtttac ggtgtaagcg atccccgcgc tctcaagcag tttgcacaga
    10081 ccggcaaaat gctcacgaga ttcctcgtcc agataatcac ctaatgccgg agcgtcgttg
    10141 agaagcgcct gcacttccgg attttttgaa tccagcacgc gcagcgggtt agtgtacatg
    10201 cggcgtttgc agtcttcgtc cagcttttct ttatgctgct caaggaatgc caccagcgca
    10261 tcgcggtaat tggcgcgtgc ttccagcgaa ccaatagagt tcagctcaag agttacgtgt
    10321 tcgaagatac ccagcgcacg ccaccagcgg gcagtgagca taatcagttc agcgtcgata
    10381 tccggacctt gcagaccgaa aacttcgcag cccaactgat ggaactgacg ataacgccct
    10441 ttctgcggac gctcgtgacg gaacatcggc ccgatatacc acagacgctg ttcctgattg
    10501 tacagaagac catgctcgat gccggcgcgt acacagcccg ccgtcccttc agggcgcaga
    10561 gtcaggctgt cgccattgcg atcctcaaag gtgtacatct ctttttcaac cacgtcggtg
    10621 acttcaccaa tcgcacgttt gaatagcggg gtctgctcta caatcggcaa gcggatttca
    10681 ctgtaaccgt agctgccgag cacgtttttc agtgtgcctt caatgcgctg ccagatggcc
    10741 gtttcgccag gcaggtaatc gttcatgccg cgaatggctt gaatgttttt tgccacgttt
    10801 attctctttc tgaatataaa aatgaaccct caacgcttcc ctcaatgttt cgggagccat
    10861 gcgggttcaa tcatacacgg gaagcgaggc gcttcccatc acgttattat ttttcaacct
    10921 gctgaacgtc aattcgacgc gcttcgtcca gctgactggc tttcgcacga atgcgtgctt
    10981 ccagctggtc gatcatatcg ttgttgtcca gacggtcttt gcgcacgcca tcttcataga
    11041 ggccgctttt cttgttgccg ccggtgacgc cgagtgtaga aaccagcgcc tcacctgggc
    11101 cattcaccac gcagccgata atcgaaacgt ccatcggagt gatgatatct tccaggcgtt
    11161 gctccagcgc gttaaccgta ccgataacat caaattcctg acgcgaacag gtcgggcagg
    11221 cgatgaagtt aatccctcgc gaacggatac gcagcgattt caaaatatcg aaaccgactt
    11281 tgatctcttc gaccggatcg gccgccagcg atacgcgcag cgtgtcgccg atgccttccg
    11341 acagcagcag acctaaacca atggcggatt ttactgcccc gctgcgtgca ccaccggctt
    11401 cggtgatccc cagatgcagc ggctgatcga tctgttttgc cagcaaacga taagactcaa
    11461 cagcgaggaa gacgtcagac gctttcacgc tgactttgaa ctgatcgaag ttcaggcgat
    11521 cgagatgatc aacatgacgc atggcggatt ccagcaacgc ctgcggcgtc ggttcgccat
    11581 acttttcttg cagatctttt tccagcgatc cggcgttaac gccaatacgg atcggaatgt
    11641 ttttatcgcg cgcacagtca accaccatgc gaatacgctc ttcattaccg atattgccag
    11701 ggttaatacg cagacaatcg acgccgtatt ccgctacttt cagcgcaatg cgatagtcga
    11761 agtggatgtc agccaccagc ggcacgttaa cccgctgttt gatgagtttg aacgcttctg
    11821 ccgcgtccat cgtcggtacg gagacacgga cgatatcagc gccaacgcgt tccagcgcct
    11881 tgatttgatt gaccgttgct tcgacatctg tcgtacgcgt attggtcatg gactgtacgg
    11941 cgatgggagc accatcgcca atcggcacat tcccaacgta aatacgtgtt gattttctac
    12001 gttgaattgg agcctggtta tgcatgaaaa atctcccgcg ttacccgtct gttactgcgc
    12061 cggtgattgt tcggcattga gggtcagacg cgcaacctgg ttagttctga taaaacgact
    12121 cagatcgaca ggtttccctt gatactggat ctgtactgcg gctggcgcac caattttcag
    12181 tttatacggt gcctggccgg ttaaattcaa attaccgtct ttacgctgca tcccgctaaa
    12241 caatttttta ccggtagcat cagtgacctc cagccagcaa tcggcagtaa agttcatcac
    12301 cagcgcattc ggatcagccg ccggcgtggt cacgccagcc tgatcggttg gcaacggcgc
    12361 agcaccatct ggcgttgttg ctgccgttgg taccggggtc gcggcggtat caacatttgc
    12421 ctgcgaaggc gaaacaaccg cattctgttg cggatcaaca gctggtgctg gcgcagttac
    12481 ggcaggtgtt tgcgtgttgg ttgcggtagt atccacagac gctggcggcg tggacgttgt
    12541 agccgggtct gtagttgtcg acgtatttaa cgggacactc tgcccctgct cgctattact
    12601 gctcagttct gccgaagatt gatcggccat agtggtgatc tcttcctgct gagctttgtg
    12661 gtcttgccac caccaggcac cgctcaggcc gataaccaca aacaacacca gccaggtgaa
    12721 ggtcatcagc cagccatcac gttttttgcg gcgtttaccg agggaaaaac tctgcattgg
    12781 cgcaactttt gcagcccgta gtggagcctg cttttccagc cctggcagca gttcttcttc
    12841 tggaatatgt accagacgcg cataagagcg gatatatccg cgcaggaatg ttgaagcaag
    12901 atcggcgggt gccttatctt cttcaatgtc gcgtaccgtg gaaaccttca ggcaaagtcg
    12961 ctcggcaacg gcctgctgac taagtcctag ttgttcgcga gcattacgca ggcgagcgcc
    13021 ggtagtaagt gcttcatttt ggtcgtgcgt ggcttcagta ttcattcgct gcaggtacgt
    13081 ttaaatgaga attgagatgc cggtgaacca tcatgctcac ccacaccgcg aaacatccgt
    13141 taagttaacc gttatcatac agtataagac cgtctgtgtg ctcgtgacaa agcccacatt
    13201 cactaaggct aacttactgt tgcatcgtta catactgcct taaagtcagc aaaaacgcac
    13261 cgttaatatt gaccagacaa ttgcaactta atacttcatt cataaaacat tacgccacgg
    13321 tacataaagt aaccgtggcg taatggctat cagaccgctt taatgtcgat ggcttcaccc
    13381 tgcatccgtt tacgcagggt acgtttcgta cggtcgataa catcgcccgc caactgacca
    13441 caggcagcat cgatatcatc accacgagtt ttacgcacaa tagtggtgaa accgtagctc
    13501 atcagcactt ttgagaaacg gtcgatacgg ctgttcgagc tgcgtccata cggcgcaccc
    13561 gggaacgggt tccacgggat caggttgatc ttacacggcg tatctttcag cagttccgcc
    13621 agttggtgcg cgtgttcagt gccgtcgtta acgtggtcaa gcatcacgta ttcaatagtg
    13681 actcggccct gattggcgtt ggatttctcc agataacggc gcaccgcagc aaggaacgtt
    13741 tcgatattgt actttttgtt gatcggcaca atttcgtcac gaatttcgtc gttcggcgcg
    13801 tgcagggaaa ttgccagtgc aacgtcgatc atatcgccca gtttatccag cgccggaact
    13861 acaccggaag tagaaagcgt gacgcgacgt ttagacaggc caaaaccgaa atcatcaagc
    13921 atgatttcca tcgccggaac gacgttgttc aggttgagca gcggctcgcc catgcccatc
    13981 atcactacgt tagtgattgg ccgctgaccg gtgacttttg ctgcgccgac gattttcgcc
    14041 gcccgccaca cctggccgat aatttccgac acccgcaggt tgcggttaaa gccctgctgg
    14101 gcggtggaac agaatttaca ctccagcgca caccccacct gcgaagagac gcagagcgtg
    14161 gcacggtcgt cttccgggat atacaccgtt tcgacgcgct gatcgccaac ggcgatcgcc
    14221 catttaatgg tgccgtcgga tgaacgctgt tcttcaacca cttccggtgc gcggatctcc
    14281 gccacctctt tcagtttgcc gcgcaacact ttgttgatgt cggtcatctc atcaaagttg
    14341 tcgcagcaat agtgatacat ccacttcatc acctgatcgg cgcggaaggg tttttcacct
    14401 aaatctttaa aaaactcccg catctgctga cggttgagat ccagcaggtt gatttttcca
    14461 tctttcgtgg tgacgttttc aggtgtgact aattgttcag acatatgcta ttccggcctc
    14521 gttattacac gttatggccc ctggagggtt gaaaaaagaa acgccccggt gagcggctgc
    14581 tcgtccgggg gcgctgcatt gtacaaattc tggcgcacgg atgccacgtt tgcacgcggc
    14641 atttacgaaa ttattaacgg gtgcgcgggc acacttcgcc ttcgccgaag aaataagcga
    14701 tttcgcgagc ggcagattcg acggaatcag aaccgtgggt accgttttcg gtcaggctgt
    14761 cagcgtaatc agcgcgcaga gtaccggcca gtgcgtttgc cggattggtc gcgcccagca
    14821 gatcgcggtg acgctgaacg gcgttttcac cttccagcac ggaaaccacg atcgggccag
    14881 aggtcatgaa ttcaaccaga ccatcaaaga acggttttcc atcgtgttca gcataaaagc
    14941 cacgagcctg ttcaacggtc aggtgcagca ttttggtgcc gacaattttg aaccctgcag
    15001 cttcaaagcg cgcaaagata ttaccaatga cgttttttgc taccgcgttc ggtttgatga
    15061 tggaaaaagt acgttcaata gccattttta cctctgtaaa ttgttctgtt gttgtctgta
    15121 ccagcgtacg agatggcgcg gattataatg agcaacaggg ccgttgacta ttgatgaagg
    15181 taacattttt ttaaaataaa atgagtttta gcaacaatca gggacaaaac agactattgc
    15241 atgacaaatt tcactgtcgc gatttgcccc acgtcatcca tcaccagcaa ttgataatca
    15301 cctttatccg tcaaatgcag ggtcacgttg cgcccgcgtt cagttaacgg ttcgccattc
    15361 agaaaccacc agcgttcacc tgcccctcca cttgattgca acggcaaagt cgcttccgct
    15421 gcgcccggta aacgtttaat aatcgcgcca tcgcgaacgc ctgtcagctg cagagggagc
    15481 tgggcatcgt gaccgtatgg cgggcaaatt gtcgaggccg gtggtaagcg cacagcgcga
    15541 cgttctgatg cgggcagcca gggttccagc ggtagcggcc agacatttac catttcttgc
    15601 ttcgcctgcg ggcagtcagc ggcgacgcgc tgccctttgt catccagcca gatggggaag
    15661 cgaatgccat taatcccttc ctgctctgac agtaacagcg ttggcggctg gctgccatcc
    15721 agcagccagg ttgccaggcg gcggcgacag ttaccgtcac cttccggcaa agactgtccg
    15781 cccggccagc agataacgcc acgagtgact gagttcgggc gcgggtcttc cggcagattc
    15841 gcactgcgcg acagtaagat attattgacc tgattcaaca atggtacggc actggcaaag
    15901 ccaaactgac caacaacggg cgtgccgtca ggtctgccag tccagatccc aatgacatag
    15961 cgagcgttaa ccccaatcgc ccaggcgtca cgatagccat agctggtgcc cgttttccat
    16021 gccagtgggg cgacgcgcgg caaggcacta tccggcaagg gttgcgcttc atcagccata
    16081 atccggcgaa tgatccacgc cgcccccgac gacattaaag gccgttcaag cagcggatcg
    16141 tcaggctgta agcgcaattt gcctgccttg ccgtggcgag caaacgcggt atacgctgcc
    16201 gccatatctt ccagttttgc accagcgccg ccgagaatga gtgaaagatt cggcgcagcg
    16261 ccgttgggca aatataacgg caatccaaca ttgcgtaact ttgccgcaaa ccgtttcggt
    16321 ccataggctt ccagcacctg cacagcaggt aagttcagcg agcgcaccag cgcctcgctc
    16381 atgctgatcg ggccatgaaa accgctatca aagttacctg gtcgataatc accggtgcgc
    16441 cgggggacgt cttgcagcag tgatgccggg tggatcaagc cttcatccag cgccagacca
    16501 taaacaaacg gtttgagcac cgatcctggc gatcggatcg cattgaccat atcaacatga
    16561 ccaaagcgtg aatcatcgtt gagatcaacc gatcccaccc agccgcgaac acgcatatcg
    16621 gtatgatcaa ccacgatcat cgccagtgag ctgcgcggtg gcaaccgccc tttccagttt
    16681 tgcgccagtt cttccagacg tcgttgaaga ccggcatcca gcgtagtaac gattttgtcg
    16741 cttttgcttt taccgagcat catgcgcgaa aacagcggtg ccagttgcgg catttgtcgg
    16801 ggtgccagcc agatgggttc ttcccttgac tcttttacct gctcacggga ccacacacct
    16861 tgcgcagcca tccgttcgag cactttatta cgcgcggctt cggcacgctc cggccaacga
    16921 tccgggcgaa gacggctggg cgcttgcggc aaaaccgcca gcattgccgc ctcggaatag
    16981 cttaaattcg caggcgactt tccgagataa gcccaacttg ccgcaccgat cccctgcaac
    17041 gtaccgccaa acggagcgcg gttaagatac aaggtcagaa tttcacgctt agacagatgc
    17101 cattccagtt gcaacgcgcg ccagagctgg cgaattttgc cgccaaatgt tttggggtga
    17161 ggatcaagca gacgagcaac ctgcatagtg agcgtgctgc caccggaaat aacccgtccc
    17221 gaagtgagat cttgccatgc tgcgcgcgcc accgagaatg gattcacccc cggatgcttc
    17281 cagaaccagc gatcttcata attgatcagc gcttcaaggt aacgtggaga aacatcttcg
    17341 attgttaccg gataacgcca gatgccgtca gcatcggcga agcgccagag cggcgtacca
    17401 tcctgcgcca cgaccactcg tgcgggattg acttcatgca gcggtagagg ccagagttta
    17461 tctgctcccc acgctgcaag aaaaaggaga aatggcgcag ctgccagcgt tatccagcag
    17521 ccgcgtttgg ttaacaagcg aggcatttac ggtcggacaa tcagcagatc ttcagccgcg
    17581 ccggtcgccc gccattgggg aacatacatt gattccacca tcggttgcgg tacctgatac
    17641 gttcccggcg tcaccgcccg cgccagatac accagcgtta ccggttggta ttcatcaacg
    17701 gcaaccgccg ccacaaagcg atcgtcacgg aactcaatgt gcttaatact cgcctgctgc
    17761 atctggttca gtaagttctg cacttcgcca ccactttgct ccaggctggc gctaccgttc
    17821 gccagattct ggttttccag ttccagaccc acaggcagca gatccacgac taacgcatcc
    17881 ggcacgctgt tactggcttt tacctgcaac cacaccagca ccagatcgcc gctacgtaac
    17941 gagtccagcg atttgctctt accatcagta ccaagaatat gacgctcgat ttgcagcaca
    18001 ttgttcgccg gtaaaggtgc ggattgcgga taaccgctgg catccatacg caaccataac
    18061 ggctgatcgc cactgttgct cacctgcaag gtgacaagtt gatcgctatt cagattgctg
    18121 ttttgcgctt tctcgcctgt cagcggctca gctgagaaag aggtttgcgc ctgccattta
    18181 cccggtaaat cctgaatcgt acgggcagcc aggaacaacg cgttactttc ctgcgtcgat
    18241 agccagcgtt caccaaacgc ctgctgcgaa agtgtgttca gcaaagtgta ctgctcatcg
    18301 ggtagcagtt tattttcttc cagcaaggag agcattaacg cgttgtcgcg cagtggacta
    18361 ccgtaatcac ccagccatat ccgctcatca ctatttctcg gcgttttcag cgccagcgca
    18421 atcgcttctt caccacgcgt cgcatcaccc atggttttca gcgcaatgcc aagttgcagc
    18481 agcggtaaac cagaagcggc atctgcacga tgctcccaga tttcacgcag cgcacccagt
    18541 ggtgcttttt gctgacgggc cagcaccagc gcagcgtaag actgaacggc gaatttactg
    18601 gctttgagat tatccgcgta cgggatcgac atcatgcccg gatcttgtaa atagcgcagc
    18661 agacgctcat taccccggtt aatggcgtct gtcggcacgc tgtaaccctg ttcgcctgcg
    18721 cggaccagga aatccatcac gtaagccgtc agccagtact cttcgtcacc gtttttatcc
    18781 cacagcgcaa agccgccgtt atcacgttgc atttgcagca aacgggaaat gccgatatcg
    18841 accgatgcac ggcgtttctc atcactgtcg cctttgatgc ccaacgcctg cagttgggct
    18901 gcgttggtat aaagtgacgg gaacaggccg ctggcggttt gctcaagaca gccgtacgga
    18961 tacgctttta actctttgat ataacgtgca atgttcagcg gtggtttgcc gctcaacaac
    19021 aattgccctt ccagcgtaac aggcgagaag ttttgcaatc catccgccgg aatcgcccat
    19081 gtctcaccag gctgtaacgc cgtaccgtaa ttaaccgttt gtgccgggaa cgccggacgg
    19141 acgccgattt tccactgctt atgctgatcg gcaacggttt cacccggtaa cgctaacccg
    19201 ctaatggtgg cctgaatttc tccatcgcca taacccggca atgctcgcac cgggataaac
    19261 agcgtagtac gcacacctgg cgctaattca acggctgcgg gtgaatcgct gaccagttca
    19321 agcaaaccac tggcggtcag ggcaacgttc agtttttgcg gtttatcggt aagattagtg
    19381 atatccagcg tcagacgcga ggtatcgcca ctcgccataa agcgcggcat gttcagttca
    19441 gcaatcaccg gtgcggcaac tatcactttg ctttcgttgc taccgaaatc atctgccgtc
    19501 caggcttgcg ccatgacgcg caattcaccg ttaaaatcgc caatcggcag tgtaaccgag
    19561 ccttcgccct gttcgttgag cgttaccgac agcgcctgct gcgcgacaat attgacgtga
    19621 ttgaccggcg gtttaccacc acgtttcagc tcatcaccat cgccaccgaa acgcagagct
    19681 gccagacgcc cctgaccttc aataacctga ccgtaaatat cgtaaatgtc tgcgccatag
    19741 cgtttctgac caaagaacgc ctgccacgga tctggcgtaa cgtagtcagt aatattcagc
    19801 acaccactat caacggcaga caccagcaca ttcacctgtt taggcttctc gccattttta
    19861 gtgctggctt taattttcac ggttaatggt tgattgggac gcatttttgc tggtgtttcc
    19921 agcgccagat cgaggcgacg gttttcatca ccaagcggca gatgcaacac accaaccgcg
    19981 cgttttggcg tcgcggagcg agatttatcg ccaggacgca ccaccagcgt actcaaataa
    20041 agatcatgac gattccaggt tttatcgacc ggaatcgtca gatccagccc ttgagccgga
    20101 acatcaatct cttgccacca cagcggccct tcactggact cgaccatcgc ataaccttta
    20161 cccgccgttg gcgcggcaat atgcaactta atggtatcgc cggggcgata actggcttta
    20221 tctagtttca gcgtgacacg gtcgggtcgc actgcgccgc tcccgtcgct gttgtcctgc
    20281 cagctatagc cagcccaaaa acgcacgcta ctgaccgctt cattcggcgc tttgacttcc
    20341 agacgataag cgccccactc taccgggaaa ctgaccttac cggtttcatc cgcctgaaga
    20401 tccagagttt gttcattttc gatcagatct ttttgatcaa actgtgactg ccagccttca
    20461 tcttctgacc agttccagta gtaatcgcgg cgttcgcgaa tcaggcgcac ctgcaagccc
    20521 gacacggctt ttttcacgcc ttgcgcatcg ctataaacga tgtcaaaagc ggcgttactg
    20581 ccttcatcaa caatgggctg tttcaccgtg ctgtcagtgc gataatcgta aaccgatttc
    20641 aaggcgaact gcggacggag ccccggcaat gcatcggcag gccagatagc ctgctcagcg
    20701 cggcgcgtca ccgggcgacc gcccgattcc agcaaactgc cctggaaaat aacctgcaat
    20761 ggggaatgcg tttccttcca ctggctttct gtagaaactt cgccgcgccc tttatcatcc
    20821 agcgtcaact gaacttcatc cagcgtgcgg gaaaggtttt cggcagctat atcgccgaat
    20881 tcaaaaccag gtaaggctga cacagcctca cgcagtgggc gcaggaaaag ttgcccttgc
    20941 aaagtattac cattagcagg cgcaccgtac aggtagtatc ccaccacgga gaatttcact
    21001 tcatcattcg gcgtaagcgg ggttttctca ccggtcagat tcagcgccat acgctctggc
    21061 ataaaatctt cgacgtggaa atcccacatc cggtactggt tatcgcccgt gttggcgcga
    21121 atatgccaca taccggttgc cgcattgcta tcgagtggcc aggtaaagtg gtagaggcca
    21181 ttctccggct gactaacgac gttcctgagt acctgcccgt caggtttaat cacgtctaac
    21241 ttgatgggtt gatcgggcaa tgctttaccg tctgcatcgc gcagtaaacc attgaggatt
    21301 accgtttcac ctgggcgata aagatcgcgt gggccaaaca tgaaaaactg tttgctatag
    21361 cctggcgcgc cagcaatgtt aaattctgct aagtccagcg ccggaagttt taaatcgagt
    21421 agcgttgtct gaccgtcttt acgtgccagc agtaatgccg catttttatc attttccagc
    21481 tgcacatgcc cctgagcgtc actggttgcc tgagtcagag tctgtccttt ctcatttaat
    21541 aaagagactt caattccttg ctgggccgcg ccgttttcca gactttgggt aaagatatcc
    21601 agacaattgt gataacggtg agctgaaacg ccgatatcac ttaacgtaaa cagcgtcgcg
    21661 ggattactgt aatcgtaacg tccagcctga ttcatcacag ccagatacac gcccgcctgc
    21721 tgaagcggtt tgatatcgcc cagcggcagc aataattttt cacgggtgtt acgcgcagga
    21781 ttgagatcaa accgtccggt gtagaccaga tccgccatct gcagcagttt gtctgactgc
    21841 cagttcgcca gcgaattgcg gtattcccat tggctaatga atgctggcag agattctggc
    21901 ttaacgcgga agaagttaac atcgacatta ttaacgttga gcgccattac cggcagccct
    21961 tcaacgactt tgccaggcag cagcgaacca cggctggcaa aaccgacgct gggttggatg
    22021 tcgcgggtag ttatagtttt ttcgtaatct ttactgaagg ttgcgttgtt gagtgctttg
    22081 acctccttgc caatagtaac gatcaaatca cgtttcggtt cgaggtggcg taaacgtagc
    22141 tctttaagat tatctgacag ctcccaggca ccatccactt tgccgctttt tttatcgacg
    22201 acatgaataa cgcgtgagaa atcctgatcc gggtcgagag ggatggagaa cgtcagcacc
    22261 agcgtggctg caccatcaag ttggacttca gagagatcca gcaatgtcag caccttaccg
    22321 gcactctgtt gggccagttt ttgtctctcc ggcacggaga gttttgctga actcgcgttt
    22381 tctgaagagg ccgctttagt aacttcagaa ggcgcatctt ttttcaccgc tgttggcgcg
    22441 ttatcgttgt tgtcgcaccc tgccagcgcc agcattagca tgcaggcggc tacgcgtaac
    22501 tttttcattt ttcatccctg ccacaatggc ccgttagcaa cgtcgaataa ttattatgcg
    22561 tgagaattta cgttatgtaa ttcagtatca cgcccataca atccgaattt tagtgagaat
    22621 tgtggctcag acagccaaat cagcctcttt cgcttgtcgc ttttcactaa acatccgaca
    22681 atttagccta accccggcaa aaatggagat gcctatgtcc acgacatggt ttgtaggagc
    22741 cgactggctc gccgaacata ttgatgaccc ggaaattcag attatcgatg cccgcatggc
    22801 gtcgcctgga caggaagatc gtaacgttgc tcaggagtat ctgaatggac atattcccgg
    22861 cgcagtgttt tttgatatcg aagcgctttc tgatcacact tccccgcttc cgcacatgct
    22921 gccgcgcccg gaaacgttcg ccgtggcgat gcgtgaatta ggcgttaacc aggataagca
    22981 cctgattgtc tatgacgaag gtaatctttt ctcagcccca cgagcatggt ggatgctgcg
    23041 cacctttggt gtagagaaag tgtcgattct ggggggtgga cttgcaggct ggcagcgcga
    23101 tgatctgctg ttagaagaag gtgcagtaga gctgccggaa ggagagttta acgccgcgtt
    23161 taatcctgaa gccgtggtga aagtaaccga tgtattattg gcaagccatg aaaatacggc
    23221 gcaaattatt gatgcccgcc cggctgcacg ttttaacgca gaagttgatg aacctcgccc
    23281 aggtttacgt cgcggacata ttcccggagc actgaatgtt ccgtggacgg aactggtgcg
    23341 cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata ttttttggtc gcggcgtcag
    23401 ctacgacaaa ccaattatcg tcagctgcgg ctctggtgta acggcagccg tggttttgtt
    23461 agcactcgcg acgctggatg tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg
    23521 gggcgcgcgg gcagatttac cggttgagcc agtgaaataa gtattttaca ggcaataaaa
    23581 aaccgccgaa tttggcggtt ttttattgct ggtctggttc gcggcctttc cagcaggttg
    23641 tattaccgta gtaatgcaag cgcgtctcag cggagacaat actcgccagt aactctcttt
    23701 ttgtcaagca aaagagagtt attattgttc tgttagtgta ttatccactg cggccctttc
    23761 cgccgtctcg caagcgggcg ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag
    23821 cgcgtctcag cgcttgctat cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt
    23881 cgcatggacc gcacaatgaa gatacggtgc ttctgtatcg tacttattgt ttctggtgcg
    23941 ctgttttccc aagtaaataa taaccggagt ctctccggcg acaatttact ggtggttaac
    24001 aaccttcaga gcagcaagta agcccgaatg ccgcccttta gggcggcata ttttagatta
    24061 tccggttttg tttaaagtca cgcaaaaaac caccccagcg acgttcatag aatggcacaa
    24121 tatgttcggt aataaagtgg ctaattcctt tttccccttt tttcacctga caaatatcga
    24181 ttggttcatc gccaggtaat gtatcggtcg ctacacttcc cgtcgcctga ataatttctt
    24241 cgatatcacc atcggcttca atgccaataa gtaaattagg ctgtgcctct tcgttctctt
    24301 taattgaaca aataaaagca cgcttcaccg gcttaatggt tttaaataag gtggtgagtg
    24361 aatcaatcat ttgtgctggc ggctctgcga cttccgataa tatcagcgat tcaccgcctt
    24421 ccaggatttc ctggctgctc agcggatttc cctcttcacc aatcaacaaa ctgatttcac
    24481 gcggcataaa ttctttaccg gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg
    24541 tcatctcaaa caatgtgcga acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct
    24601 gttgaagcgc ttctaacgag gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc
    24661 agtgctgtaa atcaagcgca ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag
    24721 gcacccagac ggtggattcc agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg
    24781 ttgctgcttt ttccagcagg tcttcaagtt cgttttttgt ttcggacata agaaccacaa
    24841 ttcattcaac gttcggggcg caaatgcctg atgcgctacg cttatcaggc ctacaaggga
    24901 ttcgcaattt gttgaatttg cagaatttgt aggccggata aggcgtttac gccgcatccg
    24961 gcatattagt ttacgccgtt aacagattag ctatcgtgcg cacaccaagt cccgtagcgc
    25021 ccgcagacca ctgttcaacc ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc
    25081 agccttgctg atagttctca acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg
    25141 ccggatacgc cgcgcttccg gtattgttca gttcggcaaa gttagacggc agctggctgc
    25201 ggtggaattc cgccagcggc agacgccaga atggttcgtt ctcttgtgag gcactcgcca
    25261 gcaaacgacc ggcaagcgca tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg
    25321 cagttttcgc cgccccggtg agggtcgccg catcaatgat catttccggt ttctgcgcac
    25381 tggcatcaat cagaccatcg gcaagcacca gacgcccttc ggcatcagtg ttcatcactt
    25441 caactttttt accgttgcga taggtgatga tatcgcccag cttgaacgca ttgccgctaa
    25501 tcaggttatc cgcacagcag aggaacagct tcacgcgctt gttcagtccg cgcgtaatgg
    25561 caaatgccag cgccccggta accgttgccg cgccgcccat gtccgacttc atcgagtcca
    25621 taaacgcagt ctgtttgatg ctgtagccgc cggagtcaaa agtgatacct ttacctacca
    25681 ggcacgcgta cactggcgct tctttatcgc cagttgggtt gtaatccagc gccagcaata
    25741 ccggagaacg ttctgaaccg cgtccgacgg tgtgcagccc catataacct tgctcacgca
    25801 gatcttcgcc tttggtgatc cgataagtca cacgatcgcc cgcgacgttg ctgatcagat
    25861 caacagcacg ctgtgccagt tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt
    25921 cacgcaccca gtcgatgatc atcaggcggt tatccagttc ctggcgctgg gcatcgtcca
    25981 gatccggcca ctccacttta cgcgtgcctt tcggggcttt gtaaccttgc cagaatgccc
    26041 agcagcgatc cgcatcccag ccttcaccgc ttaactgaac atgcttgatg cccagaccgt
    26101 caatcttgcg cgccgcacgc tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg
    26161 taatgccgtc attattaatg ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt
    26221 gggtagagag ggtaatcttc atcgcttctg tcattttagt tatccttctt tgtaaaaggg
    26281 ccgccagttg gcagccccag gatttttccg ctaaatgatt cgcgttgcag aaaggcggca
    26341 agtgagtgaa gccccaggag catagataac tatgtgactg gggtgaacga gcgcagccaa
    26401 cgcatctgcg gcgtgaagca tgacgcggaa attattcggc ctcgtccagc cagactaaca
    26461 aaatcgcttc gagaattttc tcgttggatg cctgcgggtc gtcgtcgaaa tcttccagat
    26521 cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt cggatcaaga tcaggatacg
    26581 catcgtacag tgcttcgcca atttcgcggc tatcggtcca cttaagtccc atactaacct
    26641 ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg atttcgacta ctaaatcttc
    26701 gtcggtaaca cgtgcctggc agcttaaacg gctttcgggc tccagtcccc aggctttgtc
    26761 cagcatgtcg tcttcctgct ctgagctttc tggcagcgag tcaaaacctt cacgaacgat
    26821 gcagtggcag gtggtgcaag cacaggattt ttcacaggcg tgttcaatct cgataccgtt
    26881 acgcagagct gcgtcgagaa tggtttcacc gctattagct tccagaacag cgccatcagg
    26941 acagagatcc tgatgaggca aaataacaat ctttggcata ttaaacctcg tccacggaat
    27001 ggcctttcag cgcacgacga accgactggt ccatgcggcg agcggcgaaa tcctgggttt
    27061 gtttgtctac gtttttaatc gcttgttcga tggcgtcaac gtcatcgccc tgcgccactt
    27121 cactcaggtg agcggcagca tcgtcaatga cctgacgttc tgcggcgctt aacagcgcgg
    27181 catcagcagc cagcgcgccg tgcagacttt ccagcacacg cgccgcttct actttttgtt
    27241 ctgccagcat tcgggctttt acgtcctgct cggcatagct cattgagtct ttgatcatcg
    27301 aagcgatttc gctatcggtc agaccgtaag acggtttgac ctgaatagac gcctcaacgc
    27361 cggtggattt ctccatcgcc gtcacgctca aaagaccgtc ggcatcgacc tggaacgtca
    27421 cgcgaatatg cgcaccacca gccggtagcg ccggaatacc acgcagcgca aaacgcgcca
    27481 gtgagcggca gtcctgcacc agttcgcgct caccctgcat tacatggata gacatcgccg
    27541 tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc caccggaata gtggtattac
    27601 gcggaatcac tttctccacc aggccgccca tcgtttcgag gcccagcgac agtgggatca
    27661 catcaagcag cagcatttcg ctgtctggct tgttacccac cagaatatcc gcctgaatcg
    27721 ccgcgccaat agcgacgact ttatccgggt cgatggaagt cagcggtgga cgaccgaaaa
    27781 attcgcctac ccgttcacgc accagcggca cgcgagtaga accgcccacc atcaccactt
    27841 ccagcacttc gtcagcttct acacccgcgt ctttaagcgc gcgacgacaa gccagtaagg
    27901 ttcgttttac cagtggcgcg atcagttcat tgaattgttc acggctgatt tcgccctgcc
    27961 agcccgcaac gttaacggtc acggagtccg catcgctcag cgcgattttg gctgcaatgg
    28021 cggcatccag cagttcacgc tgaacgcggt tatcgctacg atcaggaatg cccgcctgct
    28081 cgcgaatgta atccgccagc agatggtcga aatcatcgcc gccgagcgcg gaatcaccgc
    28141 cggttgccag cacttcaaac acgccgcgac ttaagcgcag aatggaaata tcaaacgtcc
    28201 cgccaccgag gtcataaaca gcgatcacgc cttcctgacc ggaatccagc ccgtaggcga
    28261 tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg caaccccgcc agacgcgccg
    28321 cgtctttggt gccctgacgc tgggcatcgt caaagtacgc cggaacggtg ataactacac
    28381 catccagctc gcctgccagg gcttcagttg cccgcgccgc cagtgctttg aggatgtccg
    28441 cagaaacgcg caccgggttc agcagccccg ccgccgtttc aatcatcggc aggccgtttt
    28501 cgctggcctg gaattgataa ggcagatgcg gatagcgttg ctggatatca gccagcgagc
    28561 gccccatcag gcgtttaaca gaactaatgg tgttggcggt gtcaagcgct gcgttagtac
    28621 gcgcgtcata acccaccgaa tgcccttgct gttgatagtg aacaacagat ggcagcaggt
    28681 gacggccttc atggtcggct aacgtttcgg cctgaccgct gcgcactgtc gccaccagcg
    28741 agttggttgt gcccaggtca ataccagccg ccagacgacg ctgatgcggc gcagcactca
    28801 aaccaggttc actaatttgt aataaggcca tgtttagctt ccagaaatta aaaatcgagc
    28861 agtttttctt cgagttgttc ggcactgctc cgcagtttat cgagaaaacg cagcttacgc
    28921 acggtatccg ccgccgcgtc ccacgcctcg ttgtctaact gttcaaccat caactgatgg
    28981 cgggtatcaa acatcttttt cacacgtttg ataaagcttt ccagccgcgc ttcatctttc
    29041 gcctgttcga tctcgtccag ctcttcgcgc agctccaact gttccatcag gaacgcggtg
    29101 tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt gtaaagaaag caaatattcc
    29161 gcgcgcatta acggatgacg cagcgtttgc caggcctggt taatggttgc agattgctgt
    29221 acggcggcga gttgttccgc ctggcttccg ctggcgaatt tatcaggatg atactgacgt
    29281 tgtagatcct gaaaacgcag gctcagcgcc tgggtatcga gttgataacg ggcaggcaag
    29341 ccaaagaagg tgaagtaatc cataacattc tcagggctgc gatccgccgc gataacggcg
    29401 aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg gggttatcgg tatgcgcatc
    29461 aaacgtggaa gctttcgccg caaccacact catctttgac gttcgggttg gtgaatttaa
    29521 acccttcgtt caggccttct tttacgaagt ccagctgcgt accgtccaga aattgcaggc
    29581 ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa cacgatgtct tccggcgtcg
    29641 gttcgtcaac aaattccagt acataagcca tacctgaaca tccggaggtt ctcacgccca
    29701 gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt atttactcgc gctgctgcac
    29761 tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt attttgcttc acgtttgctt
    29821 ttatagtccg caatggcggc tttgatcgcg tcttctgcca gaatagaaca gtgaattttc
    29881 accggcggca gttcaagttc ttcagcaata tcggtatttt tgatcgcctg cgcttcgtcg
    29941 agagacttcc ctttcaccca ttcggtgacc agggagctgg aagcgatagc agaaccgcag
    30001 ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat cgttgacttt aatctgcaac
    30061 ttcatcacgt cgccacaggc cggtgccccc accatgccgc tgccgacgtt ctcgtcgttg
    30121 ttgtcaaagg aacccacgtt acgcggattc tcgtaatggt cgataacttt ttcgctgtaa
    30181 gccattataa attctcctga ttccgatacc gattaatgat gagcccattc gatgctgttc
    30241 agatccacgc cctgcttgta catttcccac agcggagaaa ggtcacgcag acgaccgatg
    30301 gatttacgaa ctaactcgat ggtgtagtcg atctcttctt cagtagtaaa acgacctaaa
    30361 gagaaacgga tagagctatg tgccagctcg tcgttcagcc ccagcgcgcg cagcacgtag
    30421 gacggttcga ggcttgctga cgtacaggcg gaacctgaag aaactgcgag gtctttcagc
    30481 gccataatca gcgactcacc ttcaacgtag ttgaagctga cgttgagaat gttcggcgca
    30541 ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat ctttgatgcc gttccacaga
    30601 cggttacgca ggccgcgcag acgttccatc tcggtcgcca tctcttcttt tgcgatgcga
    30661 taggcctcgc ccattccgac gatctggtga acaggcagag tgccggaacg cataccgcgc
    30721 tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacgc gcggtttacg acgtacatac
    30781 agcgcaccga tacctttcgg gccatagatt ttgtgaccgg agaaagacat caggtcaact
    30841 ttcaactggc tcaggtcgat aggcagtttg cccacgctct gggttgcatc aacgtgatag
    30901 ataatgccac gagcacggca catttcgccg atagccgcga tatcctgcac cacgccgatt
    30961 tcgttattta cgtgcatgat ggaaacaagg atggtgtcat cacgcatcgc tgcttcaagt
    31021 tctttcagat cgataatgcc gttacgctgc ggtgccaggt aggtgacttc aaaaccttcg
    31081 cgctccagct gacggcaggt atccagtacc gctttgtgtt cggttttgct ggtgatgatg
    31141 tgcttgcctt ttttctgata aaagttggct gcacctttga tcgccaggtt gtcagattcg
    31201 gttgcaccag aggtaaagac gatttcacgc ggatcagcgc cgaccagatc ggcaatctga
    31261 ttacgggcga tatctaccgc ttcttcagcc tgccagccga aacggtgaga acgggaggcc
    31321 gggttaccaa aggttccgtc catcgtcata aactgcatca ttttctcggc aacacgcggg
    31381 tccaccggcg tggttgcgga gtagtcgaga taaatcggta atttcattgc tctataaact
    31441 ccgtacatca ctcaatgcaa ggaaccaggc taccggctgg atgtacgacc gtgtttacgg
    31501 agcgcgatac gccccggcct gattctaata tcttgttatc tttaacctaa ctgtttcggg
    31561 tttcagcaag ttgacaaact tacgaatctc cgggagctta cataagtaag tgaccggagt
    31621 gagtatgtgc agtcaacgca gatgcaacac gaaacaggac ggttaaatta agcgcgtaac
    31681 ttaacgtcga tcgcgtcttg tatgcgggtg cgtggcgcgt cgtgagtatg ctgacgacca
    31741 gacacatcca gcacttcctg gttattaacc agttcgccta aagtaatgtt gttgagaaaa
    31801 ccggtgagac ggtcgctcaa atcacgccac agcgcgtggg tcaggcattt atcgccgccc
    31861 tggcaaccgc ctttaccctg acaacgggta gcatctacag attcgtcaac ggcgctaatc
    31921 acttcgccaa cggcgatgct gctggcatct ttgcctaaca gataaccacc gcctggtcca
    31981 cgtacgctgg aaaccagacc atttttacgc agacgggaaa acagttgttc cagataagaa
    32041 agggaaattc cctgacgttc ggaaatatca gccaacggta ccgggcccgc ttcagagttg
    32101 agcgcaacgt caagcattgc ggtcacggca tagcgccctt tagatgtcag tctcatgtct
    32161 tacttcacct caaactcgcc cctgcccggg gttttttatt gtaaaagtgg gggtattgca
    32221 tagcagggtc aagtctgaca ttcccgagta aattggtcaa ctatttactt gactgattta
    32281 gtcgggtatt taaccttcag tgccattttt ttatcgtggc gtttgtacct gttgtccttc
    32341 cagaccatgg gcgggttgag ggcaattatt cgcattagcg ctggcctctt cccctcaccc
    32401 taaccctctc cccgaagggg cgaggggacc gtccgtgcat ttatctggct tagccctcag
    32461 gccttattac ctttattctg ctgctctata gaagccagaa tcccgcgcag gatattcaat
    32521 tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat cacctgcccc
    32581 ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg accataaaaa
    32641 cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg ctcgccattt
    32701 tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac cgccattgcc
    32761 aagttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg gcatttctgc
    32821 aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac cggggtattt
    32881 gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg ccacggcagc
    32941 gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc ttcgtcgagc
    33001 gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgctgccag ggcaatcgcc
    33061 tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc cattgttttc
    33121 atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag cacaattcga
    33181 atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta tcataaaacg
    33241 aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt taacatccag
    33301 tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg caaggcgggt
    33361 aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca gaaaggcagt
    33421 aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga cacgattcgt
    33481 aaatcttact cacagcacac catcatcacc gaagaaagcg gtgaacttga aggtactgat
    33541 caggatgttc aatgggttat cgatccactg gatggcacta ccaactttat caaacgtctg
    33601 ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt tgctgtggta
    33661 tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcagggcgc acagctgaac
    33721 ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct ggcgaccggc
    33781 ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg caaactgttc
    33841 aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc ttacgtcgct
    33901 gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt cgcggcaggc
    33961 gagctgctgg ttcgtgaagc aggcggcatc gtcagcgact tcaccggtgg tcataactac
    34021 atgctgaccg gtaacatcgt tgctggtaac ccgcgcgttg tgaaagccat gctggcgaac
    34081 atgcgtgacg agttaagcga cgctctgaag cgttaatgac tcaggcgggt gatgtcactc
    34141 acccgccctc gcctttcagg cgctattctg aaatacttcc tcaccgcttt actttctttc
    34201 gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcaca aggaaaggtt
    34261 atggcgctgc cagtgaacaa acgcgttctc aaaattctgt ttattctctt tgttgttgcc
    34321 ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc tgacgataaa
    34381 atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa ggacggtact
    34441 cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaatgc catcgcaacc
    34501 atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct ggtcagttgg
    34561 ttacccgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg taaatcaaaa
    34621 ggcacgccgt cccaggccgg attgctggac gatacgcaaa gtgccatcaa tgtggtgcgc
    34681 catcgcagtg atgtaaaccc acaacggctg gtgctgttcg ggcagagtat tggcggggcg
    34741 aatattcttg ctgttattgg tcaggatgat cgtgaaggca tacgtgcggt gatcctcgac
    34801 tccacatttg cctcttatgc aaccatcgcc aatcagatga tccccggcag tggctactta
    34861 cttgatgaga gttacagcgg cgaaaattat atcgccagcg tcagcccgat cccgctttta
    34921 ctcattcacg gtaaagctga tcacgttatc ccatggcagc acagcgaaaa gttgtatagc
    34981 ctggcaaaag agacaaaacg gttgatccta atcccggatg gcgaacacat tgatgctttt
    35041 tccgatcgtc acggcgatgt ttatcgcgaa cagatggtgg actttatcct tagcgcgttg
    35101 aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac caccttaaga
    35161 catgctaatc cactggtcag aacagtttaa gatgagaaaa attctgtgac gcttgccaac
    35221 atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc tattcttatc
    35281 aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat ctgtcctttc
    35341 ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt tgaccgccac
    35401 catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc ttgatatcag
    35461 cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg gttatgacgg
    35521 tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc actggctacg
    35581 acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg ccctgaagag
    35641 tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc tacaagcgtt
    35701 agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa acgcgcgata ttcatttcct
    35761 gtgtctgtat ctgcaatatt gtttgctgca acaccacgct ggaattacgc ctcagtttaa
    35821 tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc aggaaattgg
    35881 acgccactgg cagcgtcggg cgctccagcc tgtaccgcct gatgagccac tgtttatggc
    35941 attacttttt tccatgttgc gggttcccga tccattgcgt gatgcgcatc agcgagacag
    36001 acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg gaaatgttcg
    36061 tttttatgat gaacaggggt tatgcgatca gctttatacc cacctcgccc aggcgttaaa
    36121 tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg ccagactgta
    36181 cccacgcctg gtgcgcacca cccgcgcggc gctggccgga tttgaaagtg aatacggcgt
    36241 ccatctttct gatgaggaaa gcggtctggt cgcggtgatt ttcggtgcct ggctaatgca
    36301 ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata gcgagcgaga
    36361 agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca ttaagcatat
    36421 gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga ttattgcgcc
    36481 ttataccatg ccattaccgc tcttttcacc accgctgatc tatacggacc tgacgctgac
    36541 aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga acaactttcg
    36601 ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg ccgtggccca
    36661 gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg cccatcgcga
    36721 cggcagagta aaccgcctgt aaacggatga cttcgctacc ctggcgaaca gcaatatagc
    36781 gcatggcggc caggtggcag accgtgaagg tgccgcaatg caaaatttgc accactatca
    36841 accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg caaatcgccg
    36901 agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc agcgcaaaga
    36961 taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag gccgagtagc
    37021 cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc ccctgcaata
    37081 aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac gcagaccaac
    37141 cggtgctctc ctgctggcga cttgcccctt gtggctgaat cgtcggacgg atgagaaagc
    37201 cgagcagcat ggatgccacg cccaacgtca acagcgcgag gatcacccga taatcaaaca
    37261 tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag ccccacagtc
    37321 gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt gcatcggtca
    37381 acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc agccacgcta
    37441 cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgt cagcagtgcc agcacgcgca
    37501 aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa ctcccgagaa
    37561 aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt aaaccaatcc
    37621 ctttaagcca gacgctccag aaaggtagaa aaatgccgta actaaaaaag tatgtgaaat
    37681 agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt caggaggcgt
    37741 tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc gagcctgctt
    37801 cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg acgcatgttg
    37861 caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca atggcgatga
    37921 aatcgcgtaa tgccggcttc atctcccctt tcttccacgc catcagtaaa gcaatggaag
    37981 gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta ttcatataac
    38041 cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc agaatattcg
    38101 ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct ttaacgatcg
    38161 gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca tccagttgcg
    38221 cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc accactaatg
    38281 gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg cgcattaagc
    38341 cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt aaactcacca
    38401 gctcaatcaa ggtgtctggc tgtctgagac gaaacattgg taatactttt ggcaataaat
    38461 tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga acaattttcc
    38521 gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc tggagaaaac
    38581 attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac ggaacaccga
    38641 cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta tgcagttttt
    38701 ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt aaatgccgta
    38761 gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa ttaactattt
    38821 cacatattag caaccaacca acaacatcct tatgacacaa aaatagaagg tcaatacatc
    38881 ttatctttca ggattaaaaa atatgaccac accctcagat ttaaacattt accaactgat
    38941 tgatacccaa aatggtcggg tcactccgcg tatttatacc gacccagata tttaccaact
    39001 ggagcttgaa cgtattttcg gccgttgctg gttatttctc gcccacgaaa gccagatccc
    39061 aaaacctggt gatttcttta acacctacat gggggaagat gcggttgtcg tagtgcgtca
    39121 gaaagacggc agcattaagg cgtttctcaa ccaatgccgc caccgggcca tgcgtgtgag
    39181 ttatgcagat tgcggcaaca ctcgcgcctt tacctgtccg tatcacggct ggtcttatgg
    39241 cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac aagggttgtg
    39301 taaatcccac tggggactaa acgaagttcc ttgtgtggag agttataaag ggctgatttt
    39361 tggcaactgg gataccagcg caccgggcct gcgtgattac ctgggtgaca ttgcctggta
    39421 tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg gcgtacaaaa
    39481 gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg accagtatca
    39541 tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg atggcagcga
    39601 taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca aagatgcgct
    39661 gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac cggatgctaa
    39721 tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg aagcagaaca
    39781 acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatattt tccccacgct
    39841 ttcatggctc aacggcactg ccacgctccg cgtctggcat ccgcgcggcc ctgatcaagt
    39901 tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta aagccgcttt
    39961 tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg atgactcgga
    40021 gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca gcaaactgtg
    40081 tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg gcattactaa
    40141 ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg atcttctgag
    40201 tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg aggtgatgaa
    40261 atgagtgcgc aagtttcact agagttacat caccgcatta gccagtttct ctttcacgaa
    40321 gccagcttac tggacgactg gaaatttcgt gactggctgg agcagctcga caaagagatt
    40381 cgttacacca tgcgcaccac agttaacgcg caaacacgcg accgccgcaa aggcgtccag
    40441 ccaccgacaa cctggatttt taatgacacc aaagaccagc tggagcggcg aatcgcccgt
    40501 ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcacccgtca cttaatcagc
    40561 aactgccaga taagcgaaac cgacatccca aacgtatttg ctgtgcgggt aaattatctg
    40621 ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg tttcgacaaa
    40681 gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt cctggatcaa
    40741 gcggtaatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt tatgcgtgtc
    40801 ccgttgcgga tgtgccggag ggtgaggctc tccggatcga tacctcgccc gtcatcgccc
    40861 tgttcaacgt tggcggcgag ttttatgcca ttaacgatcg ttgcagccat ggtaatgcgt
    40921 cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta cacgccgcca
    40981 gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg ctcaccactt
    41041 atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg cagccatgag
    41101 cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag ggctggcgct
    41161 ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt cggcggcaaa
    41221 agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag gtaacgtgac
    41281 ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg gcaagctgga
    41341 ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta atactcccgc
    41401 agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg gttacctgct
    41461 gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga ttttcacact
    41521 gtcaaatgcc gccaggtatc ctggcggcgg tggcccgctg tacaccgcca gtaaacatgc
    41581 cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc gggtgaatgg
    41641 cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg ggcaaagtga
    41701 aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac cgctgcaatt
    41761 tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc ggcgcaataa
    41821 tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc gcggcattcg
    41881 ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa cgatcattat
    41941 tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc aagggttcac
    42001 cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac ctccactctc
    42061 gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg ctaactggtg
    42121 gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg gtcgcgacac
    42181 acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt ttatagcaat
    42241 cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct gctttaccct
    42301 acgccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac ggtcagtcgt
    42361 gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc agcgcagatg
    42421 taaggtgaca gtgattgaac tggcggcaac cgtcatgggc cgtaatgcac caccgcccgt
    42481 gcaacgctat cttttacagc gccaccagca ggctggtgtg cgcattctgc tcaataatgc
    42541 cattgaacat gtggtcgatg gtgaaaaagt agaactgacg ctgcaaagtg gggaaacgct
    42601 tcaggctgat gtggtgattt acggtattgg tatcagcgcc aacgagcaac tggctcgcga
    42661 ggccaacctt gatactgcca atggcattgt cattgatgag gcttgccgca cctgcgatcc
    42721 cgcgatcttt gccggtggcg atgtggcaat cactcgtctt gataatggtg cactacaccg
    42781 ctgcgaaagc tgggaaaacg ccaataacca ggcgcaaatt gccgctgccg caatgttagg
    42841 gctaccgcta ccgctactgc cgccgccgtg gttctggagc gatcagtaca gtgataactt
    42901 acagtttatt ggcgatatgc gtggcgatga ctggctttgt cgtggcaacc cggaaactca
    42961 gaaggcgatt tggtttaatc tgcaaaacgg cgtgcttatc ggtgcggtca cgctgaatca
    43021 ggggcgtgag attcgcccaa ttcgcaaatg gatccagagc ggcaaaacgt ttgatgcgaa
    43081 actgctgata gatgagaaca tcgcgcttaa atcactgtaa ccaggataat tagcgaatat
    43141 ctcaacgcct ggggcgtggc gaggtgcaag agtgtgtatt acgtttaaat cacattatct
    43201 tgcaaaggga ttggttatga acacacta
//
LOCUS       DAZSBF010000006        40580 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZSBF010000006 DAZSBF010000000
VERSION     DAZSBF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.006"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1183
                     /locus_tag="ACHXWD_000218"
     CDS             <1..1183
                     /locus_tag="ACHXWD_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR7619252.1"
                     /translation="AVTESYKPIWNWKISQYNVFDIKFETMIKPQFADRTYFSNCSPV
                     DPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLREN
                     FNEQLDTNVNIIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGDRFSNICGDK
                     FIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYPVCFYD
                     PKYKIMANHHGITFDDQRKRYIIGPITVSSDEMTNALDPVELAAIINKKHHAVINGKK
                     FKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRECIGETGT
                     VLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQ
                     L"
     gene            1373..1486
                     /gene="yhiY"
                     /locus_tag="ACHXWD_000219"
     CDS             1373..1486
                     /gene="yhiY"
                     /locus_tag="ACHXWD_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR7619253.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1549..2616
                     /locus_tag="ACHXWD_000220"
     CDS             1549..2616
                     /locus_tag="ACHXWD_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR7619254.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            2613..5348
                     /gene="rbbA"
                     /locus_tag="ACHXWD_000221"
     CDS             2613..5348
                     /gene="rbbA"
                     /locus_tag="ACHXWD_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR7619255.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5348..6472
                     /locus_tag="ACHXWD_000222"
     CDS             5348..6472
                     /locus_tag="ACHXWD_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7619256.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6545..6818
                     /locus_tag="ACHXWD_000223"
                     /pseudo
     CDS             6545..6818
                     /locus_tag="ACHXWD_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6815..7174
                     /locus_tag="ACHXWD_000224"
     CDS             6815..7174
                     /locus_tag="ACHXWD_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR7619257.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7294..7695)
                     /gene="nikR"
                     /locus_tag="ACHXWD_000225"
     CDS             complement(7294..7695)
                     /gene="nikR"
                     /locus_tag="ACHXWD_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR7619258.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7701..8507)
                     /gene="nikE"
                     /locus_tag="ACHXWD_000226"
     CDS             complement(7701..8507)
                     /gene="nikE"
                     /locus_tag="ACHXWD_000226"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR7619259.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8504..9268)
                     /gene="nikD"
                     /locus_tag="ACHXWD_000227"
     CDS             complement(8504..9268)
                     /gene="nikD"
                     /locus_tag="ACHXWD_000227"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR7619260.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(9268..10101)
                     /gene="nikC"
                     /locus_tag="ACHXWD_000228"
     CDS             complement(9268..10101)
                     /gene="nikC"
                     /locus_tag="ACHXWD_000228"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR7619261.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10098..11042)
                     /gene="nikB"
                     /locus_tag="ACHXWD_000229"
     CDS             complement(10098..11042)
                     /gene="nikB"
                     /locus_tag="ACHXWD_000229"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR7619262.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11042..12616)
                     /gene="nikA"
                     /locus_tag="ACHXWD_000230"
     CDS             complement(11042..12616)
                     /gene="nikA"
                     /locus_tag="ACHXWD_000230"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7619263.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12727..13314)
                     /gene="acpT"
                     /locus_tag="ACHXWD_000231"
     CDS             complement(12727..13314)
                     /gene="acpT"
                     /locus_tag="ACHXWD_000231"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR7619264.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13369..14418)
                     /locus_tag="ACHXWD_000232"
     CDS             complement(13369..14418)
                     /locus_tag="ACHXWD_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR7619265.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14550..15767
                     /locus_tag="ACHXWD_000233"
     CDS             14550..15767
                     /locus_tag="ACHXWD_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7619266.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15771..16328)
                     /gene="dcrB"
                     /locus_tag="ACHXWD_000234"
     CDS             complement(15771..16328)
                     /gene="dcrB"
                     /locus_tag="ACHXWD_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR7619267.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16401..17066)
                     /locus_tag="ACHXWD_000235"
     CDS             complement(16401..17066)
                     /locus_tag="ACHXWD_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR7619268.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            17287..17532
                     /gene="tusA"
                     /locus_tag="ACHXWD_000236"
     CDS             17287..17532
                     /gene="tusA"
                     /locus_tag="ACHXWD_000236"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR7619269.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17634..19832)
                     /gene="zntA"
                     /locus_tag="ACHXWD_000237"
     CDS             complement(17634..19832)
                     /gene="zntA"
                     /locus_tag="ACHXWD_000237"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR7619270.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19906..20532)
                     /locus_tag="ACHXWD_000238"
     CDS             complement(19906..20532)
                     /locus_tag="ACHXWD_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR7619271.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20673..21032
                     /locus_tag="ACHXWD_000239"
     CDS             20673..21032
                     /locus_tag="ACHXWD_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR7619272.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21035..21304)
                     /locus_tag="ACHXWD_000240"
     CDS             complement(21035..21304)
                     /locus_tag="ACHXWD_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR7619273.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21294..21890)
                     /gene="rsmD"
                     /locus_tag="ACHXWD_000241"
     CDS             complement(21294..21890)
                     /gene="rsmD"
                     /locus_tag="ACHXWD_000241"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR7619274.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22040..23536
                     /gene="ftsY"
                     /locus_tag="ACHXWD_000242"
     CDS             22040..23536
                     /gene="ftsY"
                     /locus_tag="ACHXWD_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR7619275.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23539..24207
                     /gene="ftsE"
                     /locus_tag="ACHXWD_000243"
     CDS             23539..24207
                     /gene="ftsE"
                     /locus_tag="ACHXWD_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR7619276.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            24200..25258
                     /gene="ftsX"
                     /locus_tag="ACHXWD_000244"
     CDS             24200..25258
                     /gene="ftsX"
                     /locus_tag="ACHXWD_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR7619277.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25503..26357
                     /gene="rpoH"
                     /locus_tag="ACHXWD_000245"
     CDS             25503..26357
                     /gene="rpoH"
                     /locus_tag="ACHXWD_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR7619278.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26628..27731
                     /gene="livJ"
                     /locus_tag="ACHXWD_000246"
     CDS             26628..27731
                     /gene="livJ"
                     /locus_tag="ACHXWD_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR7619279.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27930..28313)
                     /gene="panM"
                     /locus_tag="ACHXWD_000247"
     CDS             complement(27930..28313)
                     /gene="panM"
                     /locus_tag="ACHXWD_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR7619280.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            28737..29846
                     /gene="livK"
                     /locus_tag="ACHXWD_000248"
     CDS             28737..29846
                     /gene="livK"
                     /locus_tag="ACHXWD_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR7619281.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29894..30820
                     /gene="livH"
                     /locus_tag="ACHXWD_000249"
     CDS             29894..30820
                     /gene="livH"
                     /locus_tag="ACHXWD_000249"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR7619282.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30817..32094
                     /gene="livM"
                     /locus_tag="ACHXWD_000250"
     CDS             30817..32094
                     /gene="livM"
                     /locus_tag="ACHXWD_000250"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR7619283.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            32091..32858
                     /gene="livG"
                     /locus_tag="ACHXWD_000251"
     CDS             32091..32858
                     /gene="livG"
                     /locus_tag="ACHXWD_000251"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR7619284.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32860..33573
                     /gene="livF"
                     /locus_tag="ACHXWD_000252"
     CDS             32860..33573
                     /gene="livF"
                     /locus_tag="ACHXWD_000252"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR7619285.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            33857..34921
                     /gene="ugpB"
                     /locus_tag="ACHXWD_000253"
                     /pseudo
     CDS             33857..34921
                     /gene="ugpB"
                     /locus_tag="ACHXWD_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            35019..35906
                     /gene="ugpA"
                     /locus_tag="ACHXWD_000254"
     CDS             35019..35906
                     /gene="ugpA"
                     /locus_tag="ACHXWD_000254"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR7619286.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            35903..36748
                     /gene="ugpE"
                     /locus_tag="ACHXWD_000255"
     CDS             35903..36748
                     /gene="ugpE"
                     /locus_tag="ACHXWD_000255"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR7619287.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            36750..37820
                     /gene="ugpC"
                     /locus_tag="ACHXWD_000256"
     CDS             36750..37820
                     /gene="ugpC"
                     /locus_tag="ACHXWD_000256"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR7619288.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            37817..38560
                     /gene="ugpQ"
                     /locus_tag="ACHXWD_000257"
     CDS             37817..38560
                     /gene="ugpQ"
                     /locus_tag="ACHXWD_000257"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR7619289.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(38547..38987)
                     /locus_tag="ACHXWD_000258"
     CDS             complement(38547..38987)
                     /locus_tag="ACHXWD_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR7619290.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            39107..>40580
                     /gene="ggt"
                     /locus_tag="ACHXWD_000259"
     CDS             39107..>40580
                     /gene="ggt"
                     /locus_tag="ACHXWD_000259"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR7619291.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         9438 a        10470 c        11334 g         9338 t
ORIGIN      
        1 tgcagttact gagtcgtaca aacccatctg gaactggaaa atatcgcaat acaatgtatt
       61 tgatattaaa ttcgaaacaa tgataaaacc ccagttcgct gacagaacct atttctccaa
      121 ttgttcacct gttgatccca caagtaccag accgacctat tttggcgata ccgatggctc
      181 agtcggtgca gttttgtacg cgctttttgc aacgggacat ttgggaatca tggctgaagg
      241 agaaaatttc cttagccagt tgcttaatat agaagatgaa gttctcaacg tattgctaag
      301 ggagaacttt aatgagcaac ttgacactaa tgttaatata ataatcagta tcctgaacag
      361 aagagacatt gttttagaaa gtcttcagcc ttatttggtt attaataaag acgcagtcac
      421 gccatgtacg tttctgggcg accagactgg cgatcgattt agtaatatct gtggcgataa
      481 atttatcatc gatttattaa aacgcataat gagtatcaat gaaaacgttc atgtattagc
      541 tggaaatcat gaaacgaatt gcaatggtaa ctatatgcag aacttcacgc gcatgaaacc
      601 acttgatgag gatacctact ctgggataaa agattatcct gtatgtttct atgaccccaa
      661 atacaaaata atggcaaatc atcatgggat aacttttgat gatcagcgaa aacgttacat
      721 tataggaccg ataacagtct ctagtgatga aatgaccaat gctctcgatc cggtcgaatt
      781 agcggcaatt atcaataaaa agcaccatgc tgtaattaat ggcaaaaagt ttaaaaatag
      841 ccgggctatc tcttgtcgat cctttaatcg ttatttttct gtttcgacag attacagacc
      901 taaacttgaa gcactattag catgctcaca aatgttaggt ataaatcaag ttgttgcaca
      961 taacggtaat ggtggaagag aatgtatagg tgaaacgggg acagttttag gtcttaacgc
     1021 ccgtgacagt aaacatgctg gaagaatgtt cagcatgcat aattgccaaa ttaaccctgg
     1081 tgcagggcct gagataacca ctccctggaa gtcttaccag catgaaaaaa acagaaatgg
     1141 acttatgcct ttgatcagaa ggcgcacaat gttacagctc tgaaccatag acaacatgaa
     1201 tattaatgtt actatctggt tgagactgtt gataaaacgt aaaaaggaat gctctgttcc
     1261 tgcaactgaa caggcgtgaa acgctatcca acaggatgga taccgttttg tcagccagtt
     1321 ttatagatca cacttatgag tatgcgtgtt tcatttacgg ccaggaatgc tgatgatgac
     1381 aacattacta cccgttttca ctaagccttc cccgttggcg ctcaatgctc tgcgcgctgg
     1441 tcgtatttgt cgtttccttc ttatcccgga tggaagaatc cgctgaacgt cgttcggcgg
     1501 cagttgtaac acgcgtcgtt tgcttgcagg gattatggcg ggtcgattat ggataagagt
     1561 aagcgccatc tggcgtggtg ggttgtcggg ttactggtgg tggctgccgt cgtggcgtgg
     1621 tggctgttgc gcccggcggg tgtgccggaa ggctttgctg tcagcaatgg gcgcattgaa
     1681 gcgacggaag tggatatcgc cagcaaaatt gccgggcgta tcgacaccat tctggtgaaa
     1741 gaaggccagt ttgttcgcga aggcgaagtg ctggcgaaga tggatactcg cgtgttgcag
     1801 gagcagcgac tggaagccat cgcgcaaatc aaagaagcac aaagtgccgt tgctgccgcg
     1861 caggctttgc tggagcaacg gcaaagcgaa actcgtgccg cgcagtcgct ggttaatcaa
     1921 cgccagtccg aactggactc cgtggctaaa cgtcatacgc gttcccgctc gctcgcccaa
     1981 cgaggggcta tttctgcgca acagctggat gacgatcgcg ccgccgctga gagcgcccga
     2041 gctgcgctgg aatcggcaaa agctcaggta tcggcttcta aagcggctat agaagcggcc
     2101 cgtaccaata tcattcaggc ccaaactcgc gtcgaagccg cgcaagccac tgaacggcgt
     2161 attgccgcag atatcgatga cagtgaactg aaagccccgc gtgacggacg cgtgcagtat
     2221 cgggttgctg agccagggga agtgctggcg gcaggcggtc gggtgctgaa tatggtcgat
     2281 ctcagcgacg tctatatgac tttcttcctg ccaaccgaac aggcgggcac gctgaaactt
     2341 ggcggtgaag cccggctgat cctcgatgcc gcgccagatc tgcgtattcc ggcgaccatc
     2401 agttttgtcg ccagtgtcgc ccagttcacg ccaaaaaccg tcgaaaccag cgatgaacgg
     2461 ctgaaactga tgttccgcgt caaagcgcgt atcccaccgg aattactcca gcagcatctg
     2521 gaatatgtca aaaccggttt accgggcgta gcgtgggtgc gggtgaatga agaactttcg
     2581 tggcctgacg acctcgtggt gaggttgccg caatgacgca tctggaactg gttcccgtcc
     2641 cgcctgtcgc gcaactggcg ggcgtgagcc agcattatgg aaaaaacgtt gcgctgaaca
     2701 atattactct cgatattccg gcccgctgta tggtcgggct gattggtccg gatggcgtcg
     2761 ggaagtcgag cttattgtcg ttgatttccg gtgcccgcgt cattgagcag ggcaatgtga
     2821 tggtgctggg cggcgatatg cgcgacccga ggcatcgccg cgacgtctgc ccgcgcatcg
     2881 cctggatgcc gcaggggctg ggcaaaaacc tctaccacac cttgtcggtg tatgaaaacg
     2941 tcgatttttt cgctcgcctg ttcggtcacg acaaagcgga gcgggaagtg cgaattaatg
     3001 agctgctgac cagcacgggg ttagcaccgt ttcgcgatcg tccggcaggg aaactctccg
     3061 gcgggatgaa gcaaaaactt gggctgtgct gcgcgttaat ccacgacccg gaactgttga
     3121 tccttgatga gccaacaacg ggggttgacc cgctctcccg ctcccagttc tgggatctga
     3181 tcgacagtat tcgccagcgg cagagcaata tgagcgtgct ggtcgccacc gcctatatgg
     3241 aagaggccga acgcttcgac tggctggtag cgatgaatgc cggagaagtg ctggcaactg
     3301 gcagcgccga agagctacgg cagcaaacgc aaagcgctac gctggaagaa gcatttataa
     3361 atctgttacc gcaagcgcaa cgccaggcgc atcaggcggt agtgatccca ccgtatcaac
     3421 ctgaaaacgc agagattgcc atcgaagcgc gcgatctgac catgcgtttt ggttccttcg
     3481 ttgccgttga tcacgttaat ttccgcattc cacgcgggga gatttttggt tttcttggtt
     3541 cgaacggctg cggtaaatcc accaccatga aaatgctcac cggactgctg cccgccagcg
     3601 aaggtgaggc gtggctgttc gggcaaccgg ttgatccaaa agatatcgat acccgccgtc
     3661 gggtgggcta tatgtcgcag gcgttttcgc tctataacga actcaccgtg cggcaaaacc
     3721 ttgagttaca tgcccgtttg tttcacatcc cggaagcgga aattcccgca agagtggctg
     3781 aaatgagcga gcgttttaag ctcaacgacg ttgaagatgt tctgccggag tcattgccgc
     3841 tcggcattcg ccagcggctt tcgctggcgg tggcggtgat tcatcgcccg gagatgttaa
     3901 tcctcgatga gcctacttct ggtgtcgatc cggtggcgag ggatatgttc tggcagttga
     3961 tggtcgatct ctcgcgccag gacaaagtga ctatcttcat ctctacccac tttatgaacg
     4021 aagcggaacg ttgcgaccgc atctcactga tgcacgccgg aaaagtgctt gccagcggta
     4081 caccgcagga actggttgag aaacgcggag ccgccagtct ggaagaggca tttatcgcct
     4141 atttgcagga agcggcaggg cagagcaacg aagccgaagc gccgcccgtg gtacacgaca
     4201 ccacccacgc gccgcgtcag ggatttagcc tgcgccgtct gtttagctac agccgccgcg
     4261 aagcgctgga actgcgacgc gatccagtac gttcgacgct ggcgctgatg ggaacggtga
     4321 tcctgatgct gataatgggt tacggcatca gtatggatgt ggaaaacctg cgctttgcgg
     4381 tgctcgaccg cgaccagacc gtcagtagcc aggcgtggac actcaacctc tccggttccc
     4441 gttactttat cgaacagccg ccgctcacta gttatgacga gcttgatcgt cggatgcgtg
     4501 cgggcgatat cacggtggcg attgagatcc cgcccaattt cgggcgcgat atcgcgcgtg
     4561 gtacgcctgt ggaactcggc gtctggatcg acggagcgat gcccagtcgt gccgaaacgg
     4621 taaaaggtta cgtgcaggca atgcaccaga gttggcttca ggatgtggcg agccgacaat
     4681 cgacacccgc cagccaaagc gggctgatga acattgagac gcgctatcgc tataacccgg
     4741 acgtaaaaag cctgccagcg attgttccgg cggtgatccc gcttctgctg atgatgatcc
     4801 cgtcaatgct aagcgccctt agcgtggtgc gggaaaaaga gctagggtcg attatcaacc
     4861 tttacgtgac ccccaccacc cgtagcgaat ttttgcttgg caaacagctg ccgtacatcg
     4921 cgctggggat gctgaacttt ttcctgctct gcggcctgtc ggtgtttgtg tttggcgtac
     4981 cgcataaagg cagtttcctg acgctcaccc tggcggcgct gctgtatatc atcattgcca
     5041 ccggaatggg gctgctgatc tccaccttta tgaaaagcca gattgccgcc attttcggaa
     5101 cggcgattat cacgttgatc ccggcgacac agttttccgg gatgatcgat ccggtagctt
     5161 cgctggaagg gcctggacgt tggatcggcg aggtttaccc gaccagtcat tttctgacta
     5221 tcgcccgcgg aacgttctcg aaagcgctgg atctgactga tttgtggcaa ctttttatcc
     5281 cgttgctgat agccatcccg ctggtgatgg gcttaagcat cctgctgctg aaaaaacagg
     5341 agggatgatg cgccatttac gcaatatttt taatctgggt atcaaagagt tgcgcagtct
     5401 gctcggtgat aaagcgatgc tgacgctgat tgtcttctcg tttacggtgt cggtgtattc
     5461 gtcagcgacc gttacgccag gatcgttgaa cctcgcgccg atcgccattg ccgatatgga
     5521 tcaatcgcag ttatcgaacc ggatcgttaa cagcttctat cgtccgtggt ttttgccacc
     5581 ggagatgatc accgccgatg agatggatgc cggactggac gccggacgct ataccttcgc
     5641 gataaatatt ccgcctaatt ttcagcgtga tgtcctcgcc ggacgccagc cggatattca
     5701 ggtgaacgtc gatgccacgc gcatgagcca ggcatttacc ggcaatgggt atatccagaa
     5761 tattatcaac ggtgaagtga acagctttgt cgcgcgctac cgtgataaca gcgaaccgtt
     5821 ggtatcgctg gaaacccgga tgcgctttaa cccgaacctc gatcccgcgt ggtttggcgg
     5881 ggtgatggcg atcatcaaca acattaccat gctggcgatt gtattgaccg gatcggcgct
     5941 gatccgcgag cgtgaacacg gcacggtgga acacttactg gtgatgccga taacgccgtt
     6001 tgagatcatg atggcgaaga tctggtcgat ggggctggtg gtgctggtgg tatcgggatt
     6061 atcgctggtg ctgatggcga aaggtgtact gggcgtaccg attgaaggct cgatcccgct
     6121 gtttatgctg ggcgtggcgc tcagtctgtt tgccaccacg tcaatcggca tttttatggg
     6181 gacgatagcg cgttcaatgc cgcaactggg gctgctggtg attctggtgc tgctgccgct
     6241 gcaaatgctt tccggtggat ccacgccgcg cgaaagtatg ccgcagatgg tgcaggacat
     6301 tatgctgacc atgccgacga cacactttgt tagcctcgcg caggccattc tctaccgggg
     6361 tgccggattc gaaatcgtct ggccgcagtt cctgacgctg atggcaattg gcggcgcgtt
     6421 tttcaccatt gcgctgctgc gattcaggaa gacgattggg acaatggcgt aaggaaggga
     6481 aacttgactt catactgtga tgtcatacta ttgatgtcat aaagtgaagt cataaggaca
     6541 tgccatgaga accaaggtac aggctttacg gaagaaaaaa aaatacattg gatcaaatat
     6601 ttaaaactcc tgttcctcaa gggatcaagt ggtctgatat agagtcactg gttaaagcat
     6661 taggcggaga aattaaggaa ggaagaggtt cgcgttgtaa gttcatacta aatatgagcg
     6721 ttgcgtgttt ccatcggcct catccgtcgc cagataccga taaaggcgct gtagaaagcg
     6781 tgcgtgactg gttactaagt ataggggtaa aaccatgatc aaactgaaga cgccaaactc
     6841 tatggagatt gccgggcaac cagctgttat tacctacgtg ccagaactga atgcatttcg
     6901 tggcaaattc ctgggcttgt caggttattg tgattttgtc tctgacagca ttcaggggct
     6961 gcaaaaagaa ggggaacttt ccctgcgaga gtatcttgag gattgtaaag ctgcgggtat
     7021 tgagccgtat gcgcgtacag aaaagattaa gacctttacg ttacgctatc cagaatcgct
     7081 gagtgaacgc cttaataatg cggctgcgca gcaacaagtc tctgttaata cctatattat
     7141 tgaaacactg aatgagcgtt tgaatcattt ataaatttta gcggcgtaca ttacgcagaa
     7201 tatctgcctt tacaggatct atctatgcaa ctgaacttaa ttttataaat taagttcagt
     7261 taatatcttt tctttcctcg tttcagaaat aactcaatct tccttcggca agcactgcaa
     7321 atgcccatgc cgcacgccgc gctgggcgat aacgtcatcg gcaaaatgct gcacgtcgcc
     7381 catatcacct ttcagcacgg cgatttccag acagtcgtcg tgattgatat gcacatgcag
     7441 cgtggcgacg gagagatcgt ggtgatggtg ctgggtggag acaatgcggc tggctaagtc
     7501 gcgtttttcg tgttcataca cataggagag caccgcgaaa ccttgcgtgc cgtgctgctg
     7561 agtggcctcc tgggccaggg cgctacgcag aatatcgcgg atagcttcgg aacggttgtt
     7621 ataaccacga cgctggctca ggctgtccag cgtctccagt aaatcgtcat caagcgtgat
     7681 ggtgactcgt tgcatttgcg ttaaaccttt tctgtggtgc gacggcgcac ggggaatgcg
     7741 ggtaataccg cgttttgtag cacacgtccg gcgtcagagg aaaaggttaa tttatctccc
     7801 accacctggg tttcgacgat ttgtccgttg tccatcacca tcacccgctg acagaagcgt
     7861 tccaccaggc gtaagtcgtg ggtgatgaac aggcaggcgg tgccaaactg ttgttgtagc
     7921 tttttcagca ggcgaatgac gcccgcctgt aacacgagat caaggttaga aacggcttca
     7981 tccagaatca gtagtttcgg ttcgaccgcc agcgcgcgag ccaggcagac gcgctggagc
     8041 tggccgccgc ttaactgcgg tgggcgtttg tcgagaacgc tgtcatcgag atcgaccgcc
     8101 ttcagcattt cgctggcgcg cgccagttgt tcggattttt tcagtgatag caggtggcgc
     8161 atcggttcac gcaggatctc gcgcacggtt ttgcgcggat tcacggcgct gatggagtcc
     8221 tgaaatacca tctgaatatc gcggcggaac gctttacgct gggcgcgatt gagtttcgcc
     8281 agcggttcgc cacgccagct aatattcccc tgcgcgggcg attctaaacc caccagcaac
     8341 cgcgcgaggg tacttttccc gcagccgctg cgccccagca gggcgacggt ttcgccgctt
     8401 ttcagggtca gggaaacgtt attcagcacc gcctgatgtt gatgttttcc gttaaatcca
     8461 ccgtgggcat agtgatggga aaggccggag acgttaagta aagtcatgat gccagctcca
     8521 taccgtagag ggcgagatga gcggaaacca ggctgcgcgt taccgcatgt ttgggggcgt
     8581 taaacagcgt ttctacatcg ccctgttcga caattttacc ttgtgacatc accgccacgt
     8641 catccgccag acgcgccaca acgcccatat catgggtgac cagcagcatt cccggcgctt
     8701 gtttttgcat aatgctttcc agcagatcga ggatgcgcgc ctgtgctacc acgtcgaggt
     8761 cggtggtcgg ttcatcggcg atgataaacg gtgattcaca cagcaccgcc atcgcaatca
     8821 tcatgcgctg caacatgccg ccgctcatct cgaacgggta cagcttcagc acgcgcgcgg
     8881 cgttttccag ccccaccgct tctatggcag cggtaagcgt agcgtcatcg gcgggtttcc
     8941 ctaacgccag gcaggtttcc cgcgcgtggg tgtgcatggt gtgtagcgga ttaaaggcgc
     9001 tgcgcgggtt ctgcatgatg gtggcaattt tgatgccgcg cagggcgcag ggataaaccg
     9061 ctttgccatc ggctaaaatt tcccccgccg tctggcgaac gccagcgggc aaaatgccca
     9121 gcgtcgcggc gcaggttaat gatttcccgc tgccgctacc gccgactaac gccagcacgc
     9181 gcccgcgttg cagggttaac gatacaccgt gtaccagcgg ctgcgcggcc tgtagcgcga
     9241 tattacgtag ttcaatctgt tgcggcatta gtgtgcgtgc tccgtcacca gatgaggatc
     9301 cagatgatcg cgcagtgcgt cacccaccag gttaaaggcc atcacgctga taaacagcgc
     9361 cagccccggc cagaacattt gcagcggctg ggtccagata tactggcgcg cgtcgttaat
     9421 catcacgccc cattcggcgg tcggcgcggt cacaccgagg ccaaggaaag acatccccgc
     9481 gacgtgcagc atcatatggc cgatatccag cgttgccagc accagcagcg aagggatcac
     9541 cgcgcctgcc agatgatcga caaacacccg cacatggccc gcaccggaaa gccgtgacgc
     9601 cagcacaaac tcgcgttggc gtagtgaaat caccaagctg cgcaccatgc gtgcatacca
     9661 cgcccagtgc gacagggcga tggcgataat tacgttggtc agcccggtgc cgagcacgcc
     9721 aaccataaag aacgacagaa tcgaggtcgg gaaggtcata aacatatcgg cgacgcgcat
     9781 ggtggcctga tcaacgcgcc cgccaatcaa cccggcgctg ccgccaataa ccagccctaa
     9841 tgtcagcacc agcagcaggc aggccattac cgaaccgagc gacacgcggg tcgctgccat
     9901 cagccgcgag aaaatatcgc gacctaagtg atcggtgccc agccagtgct gcgcatccgg
     9961 cgaaagcagg cgcgacggca aatcaatcgc ctgggggtca tacggcagcc accactggct
    10021 ggtgagcgca atcagcgcca gcagggcgat aatgatcagc gccaggcgta ccgaccagcg
    10081 ggaagagagg aaaaagttca cgcgtgcgct ccttcatgac gacgaatgcg cgggtccagc
    10141 gcggcgttga gcaaatcgac aatcaaatta cagaccacaa aaaccaccac catcatcagc
    10201 gtaaagcact gaatcaccgg atagtcacgg ttaaaaatcg ccgacaccgc atagcgcccg
    10261 acgcccggcc aggcaaagat gttttcgata atcatcgtcc cgccaatcag ttcgccgatg
    10321 tgcatcccca cggcggtgat catcggcagc gaggcattgc gcagaatatg gcggcgttcg
    10381 gtctgtttgt cgttcaggcc gcgcagacgc gcccaagtga cgtgacgctg accggcgacg
    10441 tccagcatac tggcgcgcag cagtcgcgcg ttaatcgcca gcgacataaa ggcaatggaa
    10501 accgcaggca aaatgatgtg ctgccagccg ccgtaaccca tcgcgggtag ccattgcaga
    10561 tacaccgaaa acgccatcac cagtaaaaac gccagccaga agttaggcat cgacacgcca
    10621 agaaacgcga tgaaacgtac ggcgaaatcc ggcagacggt cgcgatggcg cgccgcccag
    10681 ataccgagcg gtacggaagt gagcagaatt aataccagcg ccgcacctgc aagttccagc
    10741 gtggcgggca ggaagttcag catatcgtcc agcaccgggc gttggctggc gaatgagata
    10801 ccaaagtcaa gatgcagcgc cttccacaac caggtaccgt actggacgta cagcggctga
    10861 tccagcccga gcatggtgcg ggtagaggcc agcatctccg gcgtcggcgg caggttagat
    10921 aaacgcagat aatcgagcgc cgggtcgccg gtaccgaggc gcagcatcag aaaaatgatc
    10981 accgaggcgg cgagcaccat cgggatcagc agcagaaagc ggcgtaatac gtaacgcaac
    11041 attaaggttt caccggttta atctgttcga acggaatttc ggtggcgatc ggcgcgtagg
    11101 ggatattacc cagctccggt tttgatacca ccatcatcga gatgtagctg ataggcagat
    11161 aaaccgcctc gtcatgcaga cgggtcaaaa tgtcgcgata cagcgcctga cgttgcgttt
    11221 cgtcatgggt cgccagcact tcgccgatct ctttatcaat cagcggcttg tcggctaatc
    11281 cttgctgtgc ctggaagtca gcgtgcgacg gtacgcgcat tgaactcagg aaggcgtgtg
    11341 gatcgtatgg cgcgccccag gtgcggtgga aaatcatgcc aaaacgaccg tcgcgctgac
    11401 gagcatagat actgctctct tcttcgccaa tcagcgagac atctgcgcca atctggcgca
    11461 tatcagcctg aatgatttcc gccatcgatt tgcttaacgc atcggtgccg atgaacgaca
    11521 gttcaatgcg cagcggctga ccatttttct cgcggatgtc tttgcccgca ggcagcgtcc
    11581 aaccggcttt ttccagcaac tctttcgctt tctgcggatc gtactggcgc ggtttcaggc
    11641 cgagattagc gtaaggcaca gaaggggcaa acagggtgtc ggcgacctgc ggggtgccat
    11701 ataacgcgtt atcaatcagc gattttttgt ttaccgcgta attaagagct tcacgtactg
    11761 ccagctcgtt ggtgggggct ttggcggtat tgagcgccag catcacggtt tcgatcggct
    11821 gtgacagttg ggtgtggtaa gccggattct ggctaaagcg ggcgaaggta tcgagcggta
    11881 ataacccttc gtttccgtac agcaggtcga tatcgccagt ttcaaacgcc accgcgcggg
    11941 tagtcgggtc cgggatgacg ttaaaggtga tctttttaat cgctggcttt tcgccccagt
    12001 agttttcgtt acggacgaag acatcgtact gattcagttt cgattcctgc aaaatccacg
    12061 gtccggtgcc aatcggcgct ttaattccgt tcatggtttc atggttttta aactgcgagg
    12121 gagcgataaa acggaaagga cggggcaggg ccagttcttg caggaaagga tagtaggcgc
    12181 ttttcagggt aatttgcagc tctgttttac tgagtgcttt aacatcaaca atctggtttg
    12241 ccagctccag ccaggcgtga cgttgacggt tatcgagcac tgcgcggaag ttttctgccg
    12301 ccgcctcggc atcgaacggt tcaccgttgg agaatttcac gtcatcacgc agggtgaagg
    12361 tccaggtttt accatcttct gaatgagtcc agctttttgc cagccacggg atcaccgaac
    12421 cgtctgcctg atatttcacc aatggttcat aaaccatgct ctgggcgaac atctggttag
    12481 gcgtgtaaag gtgcgggttt agtggcccga cattcaccgg ccaggcggta gtgatttcat
    12541 ctggtgcagc ggcatggacg ataaaagacg cacaagccag cagcgcaaat agagtgcggc
    12601 ggagtgtgga gagcatggta accccaatgg attaaaatag atggcggaaa taagtatgac
    12661 gattttaagt attcgtcata ctgattacct gttctggatc aatagatggg cagtcgggtg
    12721 ggcggatcag ttaactgaat cgatccattg cacactgtcg gcggtgagcg taaagggggt
    12781 gggggtgcag atcgcaaggc ttaaattttc tagctgacaa tggctgactg acagcgagga
    12841 gtgataggtg ctatctacgc tgacgatttg ccaggcgctg ccgccacgct gtttaacgat
    12901 ggcttctttg cgcgtccaga tgcgccagaa catttccagt tgctgatcag gatgcacggc
    12961 gtccatttca gcgtgttccc cgaggctgaa cacggcgttc gccagccagc gccagttggc
    13021 gcgcgggcga atcacttcga tatcgcagcc gacttcgcct tcatcgctca acagcagagc
    13081 gatatcgtca ccgctatggc ttaagttgaa ccatagcggc gtttccggcg cgaatgcagg
    13141 tttgccttgt tcgccataga tgatctccgg tagcggggaa agcgtgtgcg aaagcaatgc
    13201 acgccccgcc agccagcgtt cgcgtcgtgg accttgcggt gcttgctcgc gtaaacccgg
    13261 tggcagtgga gctgcgctta aggtcgaaac tttccccaga actatccgat acatatcagg
    13321 gccaacgttt aatggaaaat gaaagtgcgt atcgtatcac ttgtcgcctc atcccggtaa
    13381 ccgacttttc ggtctgcctg gccccagtaa aatcgccagt ttgctaccgc ctttggtggt
    13441 ttccatccag attttacaca cactggttaa tggcaccgaa agtagcatcc ctaccgggcc
    13501 gagcagccat ccccaaatta acaatgaaag aaataccacc atggtggaca tccccaggcg
    13561 atggcccatc atccgtggtt ctaaaatatt gccgatgacc atatggacca ctaaaaacaa
    13621 tgcgccgacc agaatacatt cgtaaacacc attaaacagc agcacctgaa tcattggcgg
    13681 tacggcggaa attaccgcgc cgatattggg cacgtagttg agcaaaaacg ccagtactgc
    13741 ccacatcagc gcaaactgca cccccatcag ctccagcccc agccagacga tgacacctgt
    13801 ccataaactg agtagcgtct tcaatgcaag atagtgcgaa acgcctttaa gtgcgcggtg
    13861 taatcctgcg atgtgaatct gtgggttatt cagcgcaaaa cgcattttgt aagggacgtg
    13921 gcgcacttca aacagcataa aaactacggt catcaccagc aaaagcacgc tcgccattgc
    13981 cccggaaagc ccggtcatta gcgctgtggt gaaggtaacc actttttccg agtccatccg
    14041 ctgcagcatt cgctccggcg acatatgcaa attaagaaaa ggcaacatct cctgcaattt
    14101 aaaaagtttg cgcgtcagct ccttattaaa cttcggcagc atagagataa attcgttaaa
    14161 cgatgccgcc agtacgccga ccagcgcggt tagtgcgatc agcatcacca ccactacaat
    14221 cgtaatggca acggggcgtt gtactccccg acgaataaac caggtaacga gcgggttgag
    14281 gacgatggca aaaaacagcg ccagtaacag ctgcacaatg atatctgccg ctgcgtgaat
    14341 gcccgcgagg atcactacca gcgaggccag cttgagcaga atgtgcatgc ccgttttatc
    14401 gggttgaggg atttccattg gggcttcctt gtgacttttt gtattaagtg tagcgggagt
    14461 cacgccagca atattctgat tcttactgct gattttcacg tcagcacgtg gtaaaaatga
    14521 aacactgttg taaaaatgtg gtgatcctca tgcccgaacc cgtagccgaa cccgcgctaa
    14581 acggattgcg cctgaatttg cgcattgtct ccattgtcat gtttaacttc gccagctacc
    14641 tcaccatcgg gttgccgctc gctgtattac cgggctatgt ccatgatgtg atgggcttta
    14701 gcgctttctg ggcaggattg gttatcagcc tgcaatattt cgccaccttg ctgagccgtc
    14761 ctcatgccgg acgttacgcc gatttgctgg gccccaaaaa gattgtcgtc ttcggtttat
    14821 gcggctgctt tttgagcggt ctgggatatc tgacggcagg attaaccgcc agtctgcccg
    14881 tcatcagcct gttattactt tgcctgggac gcgtgatcct tgggattggg caaagttttg
    14941 ccggaacggg atcgaccctg tggggcgttg gcgtggttgg ctcgctgcat atcgggcgag
    15001 tgatttcgtg gaacggcatt gtcaattacg gggcgatggc gatgggtgcg ccgttaggcg
    15061 tcgtgtttta tcactggggc ggcttgcagg cgttagcgtt aatcattatg ggcgtggcgc
    15121 tggtggccat tttgttggcg atcccgcgtc cgacggtaaa agccagtaaa ggcaaaccac
    15181 tgccgtttcg cgcggtgctt gggcgcgtct ggctgtacgg tatggcactg gcactggctt
    15241 ctgccggatt tggcgtcatc gccaccttta tcacgctgtt ttatgacgct aaaggttggg
    15301 acggtgcggc tttcgcgctg acgctgttta gctgtgcgtt tgtcggtacg cgtttgttat
    15361 tccctaacgg cattaaccgt atcggcggct taaacgtggc gatgatttgc tttagcgttg
    15421 agataatcgg cctgctactg gttggcgtgg cgactatgcc gtggatggcg aaagtcggcg
    15481 tcttactggc gggggcaggg ttttcgctgg tgttcccggc attgggcgta gtggcggtaa
    15541 aagcggttcc gcagcaaaat cagggggcgg cgctggcaac ttacaccgta tttatggatt
    15601 tatcgcttgg cgtgaccgga ccactggctg ggctggtgat gagctgggcg ggcgtaccgg
    15661 tgatttatct ggcggcggcg ggactggtcg caatcgcgtt attactgacg tggcgattaa
    15721 aaaaacggcc tccggaacac gtccctgagg ccgcctcatc atcttaaaac ttactgaata
    15781 accagcgtat taatgatgtt ttctgcggtg gtctgcgctt tttgctgatc gtcagctggc
    15841 agagtaattt gcatggtcag cagttgatta cccacgttac ccagaataac ggaagagtac
    15901 gccgtctggc ctttcgcgga gataatactg tctaactgct gcattttgtg acctttcagc
    15961 tcaatggctt tgttggttac cacttgcagc tgcggatcgc ggctacgttg ctgatcttcc
    16021 agacgcttcg ccaacaccgc cagatcttct ttcggatcat cgcccatgat gacgatgact
    16081 gctttctgcc cggtggcgtc ggaccagaca tgcatgttat tggcctgcgt tcccagctta
    16141 ccgctctggt cggtcatatc cgctggcagc gagaaactta acttgccatc aagcaggttg
    16201 acgggattcc cggtagcgtt actttccgcg accgaaccct gcgccgtagc gttagtgtct
    16261 ttatcatcac aggccgcaag ccccataacc agcaggccaa ttccgacata tttaaccaga
    16321 ttgcgcattg acttcttcct ttcgataaac ggccataacg gctcattcat ccatcttatc
    16381 acaactctga taacgaacct ttaactcgcc tgcaaagcgt tgatttcgga tttatctgcc
    16441 agtcttttca acagaatatt gagtaatacg ccatacattg gcaggaagaa aacgatactg
    16501 attaacactt tgaaacagta atcgaccagc gcgatttcca tccagtgttc agccataaag
    16561 gcatccgggc tacgccagaa ggcaatgaag aaaaaggcca gcgtgtcgct gacgttaccg
    16621 aacagtgtgg acgctgtcgg tgccagccac cagcggcgac tctgacgcag gcggttaaaa
    16681 acgtgcacgt cgaggatctg tcccagcgcg taggccatga aactggcggt ggcgatacgg
    16741 gcgacaaaca ggttgaagtg ggcgagtgcg ccgaatccct gccaggaacc catatagaat
    16801 agcgacgaga tgacgtagga gatcagcaac gccgggatca tcaccgcaaa gatgatacga
    16861 cgggccagcg gtgcgccaaa aatacgcacg gtcaggtcgg tagcaaggaa aataaacggg
    16921 aagctaaacg cgccccaggt ggtatggaaa cccaaaatgg agacgggaag ctgaaccaga
    16981 tagttactgg aggtgatcac cagcagatga aataacgata accagaacaa cgccttatag
    17041 cgttgagttt gcgagaaaac gttcatattg tacctttttg attaaccatt ggggtgaggg
    17101 aacccaatac gtacgacacg tctgttacct taacataata acgtgcttac ccttttttcg
    17161 agccgccgca tgatactgct ttacgttgac aatgcaatgg ttaattttca cgcaatcgtt
    17221 aacctggttt gcttacggac tcacagggcg taaactagcg ccgttttttt atgtgatgag
    17281 aagaaaatga ccgatctctt ttccagccct gaccacacac tcgacgcact tggcctgcgc
    17341 tgcccggaac ccgtaatgat ggtgcgcaaa actgtgcgca atatgcagcc tggcgaaacg
    17401 ttgctgatta tcgccgacga tccggccact acccgcgata ttcctgggtt ttgtaccttt
    17461 atggaacacg aactggttgc gaaagagacg gatggactgc cttatcgtta tttgattcgt
    17521 aaaggcggtt gatggggaaa gattggcttc gatgccgcct tttcccctca ccctaaccct
    17581 ctccccagag gggcgagggg accgatcgcg ctcaatgttg cgatcggttt gccttatctc
    17641 ctgcgcaaca atcttaacgc attcgctgtc accagcaccg tcgcacccgt atctgccagc
    17701 accgccagcc acaggccggt catccctaac agcgtggtga cgaggaagat ccctttcagc
    17761 cccagcgcaa tggtgatgtt ctggcggata ttggcgtgag tggcgcgtgc cagttcaatc
    17821 atttgcacca ggccgcgcag gtggttatgg gttaatgctg catcagcggt ttccagcgcc
    17881 acgtctgtgc cactgcccat tgcaatccca atggcggcag ctttcatcgc tggcgcgtcg
    17941 ttaataccat caccgaccat cgccaggggc gcatgttgat tcagcttggt caccgctttg
    18001 actttatctt ccggcaatag gcccgcttta aactccagcc ccagctcccc ggcgattgcc
    18061 gccgctgcac gtggattatc gccggtgagg atcactcctt tgacccccag cgcgttcagt
    18121 tcactgatgg cagttgcagc atcggcgcgc agggtatcct gcaatgcaat gatacccagc
    18181 acgtcatcat tacgtactac cagcacaacc gtttgcccgg cgctttccag ttcattaatc
    18241 aaaccagcaa atgcatcagc gggatgtttc ccggcagcgc aaatcaatac gcgctcaccg
    18301 ttaacctgcg cttcaatgcc agacccgacc agcgcccgct gtgattcggc ggtgggaatg
    18361 gcgagttcag caacctgtgc ttcgcgtacg atggcttgcg ccagtggatg cgtcgcgcct
    18421 tgctcgaccg ccgccgccag tgtcagcagt tcagattcac taatacccgt tgccggatga
    18481 atcgcggtaa cgcgcggttt accgacggtc agcgtaccgg ttttatcaaa cgccacctga
    18541 gtaacacgac ctagttgttc cagcgccgct ccgcctttaa tcaacgcccc acgacgcgct
    18601 gccgccgcca gcccggaggt aatcgccgca ggcgttgaga taactaacgc acacgggcag
    18661 ccaatcagca gcagcgtcag ccctttataa atccactcct gccagctggc ggcaaacagc
    18721 agcggtggca ccagcgtcac cagcagagcg acggccataa tcgcgggcgt atagatacgg
    18781 ctgaaacggt cgataaaccg ctcaatgggg gcgcgacgct cttcggcttc ttcgatcagt
    18841 ttcagaatcc ggtcaatggc gctggcaccc ggttctgaca gcacttccag cgttaccaga
    18901 cggtctacgc tggtggctcc tgcaggaact ttatcgcccg tcgcgcgctc caccggaatg
    18961 gattctccgg tcagggcgct ttcatcaaaa ctggcaaacg gtgagagcag tttaccgtcg
    19021 gcaggcaaac gcccacctgc ggcgacttca atcacatcgc caggccgcag gctgttaatc
    19081 gccacctctt cccgctcacc gttacgcagg cgcgtggcgg tttctggttt cagcgccatt
    19141 aacgcgctaa cgccctgacg cgcgcggctg gcggcccagc cttccagtcg ttcaccaatc
    19201 aaaaacagca gcaacaccat cgcagcttca gccgttgcgc caataaacag tgcaccaata
    19261 gcggctacgc tcattaaggt ttcaatggcg aagtagctgc cggatttgat cagccgtaat
    19321 gcctgacgag caatcgggta cagcccaacc agcgtggtcg cgataaacgc cagttgcccg
    19381 aacggatgat tgaactgctc cagaccccag ctgattgcca tcatcacgat tagtgtaatc
    19441 agcggcagat tctctttcag gcgcgatgct tgtggctcgt cggcggcctg ctcatcgcgc
    19501 agggaatagc ccgctttttg caccgcagat tcaacttgtg cacgaatgtc attgtcggca
    19561 tcaaccacca gtttttcggt ggcgaacaac acctgcacct gattcacacc tgcaagctgg
    19621 cgcacggcat tttctacctt gcgcgcacag gcggcgcagt ccatgccact gactttccag
    19681 ctatagcggg tgccggagac gttttcagag agcgttggcg agctggagca tgcgccgtcg
    19741 cagcaacagt cgttggcgtt ctgtaccgtg gttagcggtt tgaacgcagc aaattgaggg
    19801 gctttcttgc cgtgattgtc aggtgtcgac atggcatact ccggttaagt tttttctcat
    19861 taaccgaagg atacactctg gagtcgactc cagagtcaag ttttatcaga gatacagtga
    19921 gcggatgatg aggaagtgac cggcaaagta gcaggccgca gcaatcgcgt tatccgcacg
    19981 gaagcgtcgg cgatagtggc tccccagcca gacaaagtta ctgataaaca gcaacgaagc
    20041 gccaacaaac gcagagagcg ccggagcggt cggacggaag aaccacagtt cacctgccag
    20101 ccacaccatc accagcgtca tgccgataaa cgtgcagata ggccaacgat actcttccag
    20161 ccgcgtccag ataatcgcca gtaacagcgc acccagaacc agcaacacca gtggcagagg
    20221 ccagaagaaa gagagcgtca tctgactggc gaaatagatg gtgtacagca ggtgcgagag
    20281 gaaaaacgcg ccgatggcgt acatcagacg ttgacgtggc aacagggtta gcgcatcgcc
    20341 cagcagtgag gcgcacagcc ctgccagcac cagatagcta atggcgtcga acatcggcgc
    20401 ttgccaggcc agtaacagca ggagaagaag ggttaacggt ttaaacaccc agcgttgcca
    20461 ggttggccca cgatacgatg catccacaga tagccatgcg gaaagacaga cagcgataaa
    20521 cgaccaaagc atcttagttc cttgaattcg ttattttttc gtaagcggaa tgcctacggt
    20581 cttgcttcca gtttagtggc gagggtggac ggatgacaac accataactg gcagggtatg
    20641 cttatttccg cattttccga tgagggataa agatgagcaa accacctctt ttctttattg
    20701 ttatcattgg cttaattgtc gtcgccgcat cgtttcgttt tatgcaacag cgacgggaaa
    20761 aagctgataa tgatatggct ccgctccagc aaaagctggt ggtggtgagc aacaagcggg
    20821 aaaaaccgat taacgatcgc cgttcgcgcc agcaggaagt gactccggca ggcaccagta
    20881 tgcgctacga ggcaagcttc aaaccgcaaa gcggaggaat ggagcagacg tttcgcctcg
    20941 acgcccagca gtaccacgcc ctgacagtgg gcgataaagg tacgctgagc tataaaggaa
    21001 cgcgctttgt tagctttgtt ggcgaacaat aaggttattt tttaacttta aatttcttct
    21061 gccagaccag cagttcaaac acgccgaaaa ggaaaatccg taccttttcg gcggtggtca
    21121 tctgcgggcc atctttcggt aaagtggatt tcaatagcgc cagctgcata ccatgcatca
    21181 gcacggtaaa aatcagcgcc acgttaacga aaatatttag cgggcgcggg aagggatgca
    21241 ccagattgag gattaaaaat ccccaaacgc agagcattaa caaacgacca atattaatca
    21301 gcatcacttt ctccttgtgc ttcgcgttga tacagccgat aagccacctg acccgccact
    21361 ttttcccgat gtaacgacca gtttgccgga acggtgggca gaccgttttc gacttcgctt
    21421 tcgacataaa tcagggcttc gtcagccagc cagccgttat cttccagtaa atttatcgtc
    21481 tcttctaaca agccacggcg gaacggtgga tcgacaaaca cgatattatg cggtgtgcct
    21541 ttttgcgcca ggaatgacat cgcgttgctg ttcaccacgc gtgcattgcc tgcttttagt
    21601 gtcgccagat tcttaattaa ctgctgagaa actgcacgat ccatctcaat taacgtggcc
    21661 cccgcagcgt agcgcgataa cgcttccagc cccagcgcgc cgctcccggc gaagcaatcc
    21721 agacattggg cgtcaacaat gaccggagcc agccagttaa acaacgtttc gcgtacgcgg
    21781 tcggtggtgg ggcgcagacc tgggctatca ggaaccggga gtttacggcc tcgccactgc
    21841 ccgccaataa tgcggatttg gccgctgccg gaatgattcg gttttttcat gataaattgc
    21901 tcaatccgcc agataacaca tacttgcagg cggtgatgtg aattaagtta agtgatagac
    21961 tatttcatca tttttttagc tgctatgtac atagcgttaa cgctgtgcca tgaagcaaca
    22021 gcgaggagtg tagtcgcaaa tggcgaaaga aaaaaaacgt ggcttttttt cctggctggg
    22081 ctttggtcaa aaagagcaga ccccggaaaa agagacagaa gttcagaatg aacaaccggt
    22141 tgtagaagaa atcgttcagg cgcaagagcc tgtgaaggct tcggaacaag ccgttgaaga
    22201 gcagccgcag gcgcatactg aagccgaggc ggaaactttt gctgccgacg ttgtggaagt
    22261 cactgaacag gttgttgaaa gtgaaaaagc gcagcctgaa gcggaagttg ttgcacagcc
    22321 ggaaccggtc gtagaagaaa cgccggagcc agtggctatc gaacgtgaag agctgccgtt
    22381 gccggaagac gtcaacgccg aagaggtttc gccagaagag tggcaggctg aagcggaaac
    22441 cgtagagatt gtcaaagcgg cggaagaaga agcggctaaa gaagaaatca ccgacgaaga
    22501 gctggaagca caggcgctgg cagccgaagc agcagaagag gcggtgatgg tggttcctcc
    22561 ggcagaagaa gagcagccgg tggaagaaat cgctcaggag caggaaaaac caaccaaaga
    22621 aggtttcttc gcgcgcctga aacgcagcct gttaaaaacc aaagaaaatc tcggttccgg
    22681 atttatcagc ctgttccgcg gtaaaaaaat cgacgatgat ctgtttgagg agctggaaga
    22741 gcagcttttg atcgccgatg tgggcgtgga aaccacacgt aaaattatca ccaatctgac
    22801 ggaaggcgca tcccgcaagc agcttcgtga tgccgaggcg ctctatggcc tgctgaaaga
    22861 agagatgggt gagattctgg cgaaagtcga tgagccgctg aatgttgaag gtaaaacgcc
    22921 gttcgtgatc ctgatggtgg gcgtcaacgg tgtgggtaaa accacgacga ttggtaagct
    22981 ggcacgtcag tttgagcagc agggtaaatc ggtgatgctg gcggcgggcg atactttccg
    23041 tgcagcagcg gttgaacagc ttcaggtctg gggtcagcgc aacaatattc cggtgattgc
    23101 ccagcatacc ggggcggatt ccgcctctgt tatcttcgac gccattcagg cagctaaagc
    23161 gcgtaatatc gacgtcctga ttgccgatac cgccggacgc ctgcagaaca aatcgcacct
    23221 gatggaagag ttgaagaaaa tcgtccgcgt gatgaagaaa ctcgacgttg aagcgccgca
    23281 tgaagttatg ctgactattg atgccagcac cgggcagaac gcggtaagcc aggccaaact
    23341 gttccacgaa gctgttggct taaccggcat cacgctaacg aaactggacg gcacggcgaa
    23401 aggcggggta attttctcgg tggctgacca gtttggtatc cctatccgct acattggtgt
    23461 cggcgaacgt attgaggatt tgcgtccgtt taaggcggac gactttatag aggcactttt
    23521 tgcccgagag gattaacaat gattcgcttt gaacatgtca gcaaggctta tctcggtggg
    23581 agacaggcgc tgcagggcgt tacgttccat atgcagccgg gtgagatggc gtttctgacc
    23641 ggtcattccg gcgcagggaa aagtaccctc ctgaagctga tctgtgggat tgagcggccc
    23701 agcgccggga aaatctggtt tagcggccat gacatcacgc gtctgaaaaa ccgtgaagtt
    23761 ccgtttctgc gccgccagat tggcatgatt ttccaggatc accatctact gatggaccgt
    23821 actgtctacg ataacgtggc gatcccgctg attatcgctg gtgccagcgg tgacgatatt
    23881 cgtcgccggg tgtcggcggc actggataaa gtcgggctac tggacaaagc gaagaacttc
    23941 cctattcagc tttccggcgg tgaacaacag cgtgttggca ttgcccgcgc ggtggtgaat
    24001 aagcccgcgg tactgctggc ggacgaaccg accggtaacc tggacgacgc gttgtcggaa
    24061 gggattctac gtctgtttga agagtttaac cgcgttgggg taaccgtatt gatggcaacg
    24121 cacgacatca acctgatttc acggcgttcc catcgcatgc tcaccctgag cgatggtcac
    24181 ttgcatggag gcgtgggcca tgaataagcg cgatgcaatc aatcatattc ggcagtttgg
    24241 tgggcgtctg gatcgcttcc gtaaatcggt cggcggctca ggcgacggcg gtcgtaacgc
    24301 accgaaacgc gcgaaatcct cgccaaaacc ggtaaatcgc aaaaccaacg ttttcaacga
    24361 acaggtgcgc tatgccttcc acggcgcatt gcaggatctg aaaagcaaac cgttcgccac
    24421 gtttttaacg gtgatggtta tcgccatctc cctgacgctg cccagcgtct gttatatggt
    24481 gtacaaaaac gttaatcagg cggcgacgca gtattatccg tcaccgcaaa tcactgttta
    24541 cttgcaaaaa acgctggacg atgacgccgc tgcgggcgtg gtggcacagt tgcaggccga
    24601 gcaaggcgtg gagaaagtga actatctttc tcgtgaagac gcactgggcg agttccgtaa
    24661 ctggtctggt tttggtggtg cgctggatat gctggaagaa aacccgcttc cggcagtggc
    24721 ggtggtgatc ccgaaactcg attttcaggg gacggaatca ctcaatacgc tgcgtgatcg
    24781 tatcacgcag attaacggca ttgacgaagt gcggatggat gacagctggt ttgcccgtct
    24841 ggcggcgttg accgggctgg tcgggcgcgt ttcggcgatg atcggcgtgt tgatggtggc
    24901 ggcagtgttc ctcgtcatcg gcaacagcgt acgtctgagc atctttgctc gccgtgactc
    24961 catcaacgtg cagaaactga ttggtgcgac ggatggtttc atccttcgcc cgttcctgta
    25021 tggtggcgca ctgctgggat tttctggcgc attgttgtca ttaattttgt cagaaattct
    25081 ggtgctgcga ttgtcatcgg cggttgcgga agtggcacag gttttcggaa cgaagtttga
    25141 tatcaatgat ttatcgttcg atgaatgcct gctattgctg ctggtatgct cgatgattgg
    25201 ctgggtggca gcgtggcttg ccacggtaca acatttacgc cactttacgc ctgaataata
    25261 aaagcgtgtt atactctttc cctgcaatgg gttccgtagc agggaaagag atcccgttgt
    25321 ctcttcccgg tatttcatct ctatgtcaca ttttgtgcgt aatttattca caagcttgca
    25381 ttgaacttgt ggataaaatc actgtctgat aaaacagtgg atgataacct cgttgctctt
    25441 aagctctggc acagttgttg ctaccactga agcgccagaa gatatcgatt gagaggattt
    25501 gaatgactga caaaatgcaa agtttagctt tagccccagt tggcaacctg gattcctaca
    25561 tccgggcagc taacgcgtgg ccgatgttgt cggctgacga ggagcgggcg ctggctgaaa
    25621 agctgcatta ccatggcgat ctggaagcag ctaaaacgct gatcctgtct cacctgcggt
    25681 ttgttgttca tattgctcgt aattatgcgg gctatggcct gccacaggcg gatttgattc
    25741 aggaaggtaa catcggcctg atgaaagcag tgcgccgttt caacccggaa gtgggtgtgc
    25801 gcctggtctc cttcgccgtt cactggatca aagcagagat ccacgaatac gttctgcgta
    25861 actggcgtat cgtcaaagtt gcgaccacca aagcgcagcg caaactgttc ttcaacctgc
    25921 gtaaaaccaa gcagcgtctg ggctggttta accaggatga agtcgaaatg gtggcccgtg
    25981 aactgggcgt aaccagcaaa gacgttcgtg agatggaatc acgtatggcg gcacaggaca
    26041 tgacctttga cctgtcttcc gacgacgatt ccgacagcca gccgatggca ccggtgctct
    26101 atctgcagga taaatcatct aactttgccg acggcatcga agatgataac tgggaagagc
    26161 aggcggcaaa ccgtctgacc gacgcgatgc aaggtctgga cgaacgcagc caggacatca
    26221 tccgcgcgcg ctggctggac gaagacaaca agtccacgtt gcaggaactg gctgaccgtt
    26281 acggtgtttc cgctgaacgt gtacgccagc tggaaaagaa cgcgatgaaa aaactgcgcg
    26341 ctgctataga agcgtaattt ccgctattaa gcagagaacc ctggatgcga gtccggggtt
    26401 tttgtttttt gggcctctac aataatcaat ttcccctccg gcaaaacgcc aatccccacg
    26461 cagattgtta ataaactgtc aaaatagcta ttccaatatc ataaaaatcg ggtatgtttt
    26521 agcagagtat gctgctaaag cacgggtagc catgcataaa acgaaataaa gtgctgaaca
    26581 acaacatcac aacacacgta ataaccagaa gaatggggat tctcaggatg aacataaagg
    26641 gtaaagcgtt actggcagga tgtatcgcgc tggcattcag caatatggct ctggcagaag
    26701 atattaaagt cgcggtcgtg ggcgcaatgt ccggtccggt tgcgcagtac ggtgaccaag
    26761 agtttaccgg cgcagagcag gcggttgcgg atatcaacgc taaaggcggc attaaaggca
    26821 acaaactgca aatcgtaaaa tatgacgatg cctgtgaccc gaaacaggcg gttgcggtgg
    26881 cgaacaaagt cgttaacgac ggcattaaat atgtgattgg tcacctctgt tcttcatcaa
    26941 cgcagcctgc gtctgacatc tacgaagacg aaggcatttt aatgatcacc ccagcggcaa
    27001 ccgcgccgga gctgaccgcc cgtggctatc agctgatcct gcgcaccacc ggcctggact
    27061 ccgaccaggg gccgacggcg gcgaaatata ttcttgagaa agtgaaaccg cagcgtattg
    27121 ctatcgttca cgacaaacag caatacggcg aaggtctggc acgagcggtg caggacggcc
    27181 tgaagaaagg caatgctaac gtggtgttct ttgatggtat caccgccggg gaaaaagatt
    27241 tctctacgct ggtggcgcgt ctgaagaaag agaatatcga cttcgtttac tacggcggtt
    27301 accacccgga aatggggcaa atcctgcgtc aggcacgcgc ggcagggctg aaaactcagt
    27361 ttatggggcc ggaaggtgtg gctaacgttt cgctgtctaa cattgcgggc gaatcagcgg
    27421 aagggctgct ggtgaccaag ccgaagaact acgatcaggt tccggcaaac aaacccattg
    27481 ttgacgcgat caaagcgaaa aaacaggacc caagcggcgc attcgtttgg accacctacg
    27541 ccgcgctgca atctttgcag gcgggcctga atcagtctga cgatccggct gaaatcgcca
    27601 aatacctgaa agcgaactcc gtggataccg taatgggacc gctgacgtgg gatgagaaag
    27661 gcgatctgaa aggctttgag ttcggcgtat ttgactggca cgccaacggc acggcgacag
    27721 atgcgaagta atcattaatc ggcaactttg ggttgccgcc aaattgctaa tatcgagtac
    27781 gttgcttcat gccggatgcg gcgtaaacgc cttatccggc ctataagatc caaagaaatc
    27841 agtaaattgc aacacacatt gtaggcctga taagcgtagc gcatcaggca atttctcgtt
    27901 ggtcatcact tttgaaatca acttgacgat taacgcttct cccagccgcc ctgttgcgcc
    27961 gtaaatccca gtgcctgcat aaacgctgtc atcacaccgc gatcttccac gcctgcatcc
    28021 gccatccacc agcatgaaac gccaggattg ttacgcaaaa cctcttccag cagatattgc
    28081 cccacaccgc gacgtcgggt gacttcccgc acacgcaggg aatccagtgc tccctcggtg
    28141 ccgcttaagg ttacccgtac ggcagcgagc aggcgctcgt taaaacgcgc ggcgtagata
    28201 cggtggttat cgtcaacctg taaagaggaa ggggaatact ccggccagat cttttgcagg
    28261 tcaatccggt cctggtcgct aaagttttct aatcgaatga tggtcagctt catgggtaac
    28321 ccgtgtaaat cacaaaagtg gaaccagtgt agcgaaataa tttaatcgga ggctttctct
    28381 tttttatttc ttttggcagg tgattaattt tttaacagca ataattacaa agttaaaaca
    28441 ttagagaatg aaaaataccc agcataatcc cctgaatggt agtgaattat tccgcccttt
    28501 gtgccgttat tttatgctga caaaggcact tttttctgtt tatctatcaa taaattcaga
    28561 atattatctg ttcttaatcg actgaaaaat agggatttta atcactgtta tcacaaaata
    28621 ctgcgctaaa cccttaatca gacaggcaaa aacagtgcag tataaaaaaa gaacagtctg
    28681 atttgttaac acataaaaac aaagcaacac aacatcacga atggggattt ttgactatga
    28741 aacggaatgc gaaaactatc atcgcaggga tgattgcact ggcaatttca cacaccgcta
    28801 tggctgacga tattaaagtc gccgttgtcg gcgcgatgtc cggcccgatt gcccagtggg
    28861 gcgatatgga atttaacggc gcgcgtcagg cgattaaaga cattaatgcc aaagggggaa
    28921 ttaagggcga caagctggtt ggcgtggaat atgacgacgc ctgcgacccg aaacaagccg
    28981 ttgcggtcgc caacaaaatc gttaatgacg gcattaaata cgttattggt catctgtgtt
    29041 cttcttctac ccaacctgca tcagatatct acgaagacga aggtattctg atgatctcgc
    29101 cgggagcgac caacccggag ctgacccaac gcggttatca acacattatg cgtactgccg
    29161 ggctggactc ttcccagggg ccaacggcgg caaaatacat tcttgagacg gtgaagcccc
    29221 agcgcatcgc catcattcac gacaaacaac agtatggcga agggctggcg cgttcggtgc
    29281 aggacgggct gaaagcggct aacgccaacg tcgtcttctt cgacggtatt accgccgggg
    29341 agaaagattt ctccgcgctg atcgcccgcc tgaaaaaaga aaacatcgac ttcgtttact
    29401 acggcggtta ctacccggaa atggggcaga tgctgcgcca ggcccgttcc gttggcctga
    29461 aaactcagtt tatggggccg gaaggtgtgg gtaacgcatc attgtcgaat attgctggcg
    29521 atgctgccga aggcatgttg gtcactatgc caaaacgcta tgaccaggat ccggcaaacc
    29581 agggcatcgt tgatgcgctg aaagcagaca agaaagatcc gtccgggcct tatgtctgga
    29641 tcacctacgc ggcggtgcaa tctctggcga ctgcccttga gcgtactggc agcgatgagc
    29701 cgctggcgct ggtgaaagat ttaaaagcta acggtgcaaa caccgtgatt gggccgctga
    29761 actgggatga aaaaggcgat cttaagggat ttgattttgg tgtcttccag tggcacgccg
    29821 atggttcatc cacggcagcc aagtgatcat cccaccgccc gtgaaatgcg ggcgggttta
    29881 gaaaggttac cttatgtctg agcagttttt atatttcttg cagcagatgt ttaacggcgt
    29941 cacgctgggc agtacctacg cgctgatagc catcggctac accatggttt acggcattat
    30001 cggcatgatc aacttcgccc acggcgaggt ttacatgatt ggtagctatg tctcctttat
    30061 gatcatcgcc gcactgatga tgatgggcat tgataccggc tggctgctgg tggcggcggg
    30121 attcgtcggc gcaatcgtca ttgccagcgc ctacggctgg agtatcgaac gggtagccta
    30181 tcgcccggtg cgtaactcta agcgcctgat tgcgctcatc tcggcaatcg gcatgtccat
    30241 cttcctgcaa aactacgtta gcctgaccga aggttcgcgt gacgtggcgc tgcccagtct
    30301 gtttaacggt cagtgggtgg tggggcatag cgaaaacttc tctgcctcta ttaccaccat
    30361 gcaggcagtg atctggattg ttactttcct cgccatgctg gcgctgacga ttttcattcg
    30421 ttattcccgt atggggcgcg cctgccgtgc ctgcgcggaa gatctgaaaa tggcgagcct
    30481 gcttggcatc aacaccgacc gggtgattgc tttgaccttt gttattggcg cggcgatggc
    30541 ggcagtggct ggcgtactgc tcggtcagtt ctacggcgtc attaacccct atatcggctt
    30601 tatggccggg atgaaagcct ttaccgctgc ggtgctcggt gggattggca gcattccagg
    30661 ggcgatgatt ggcgggctga tcctggggat tgcggaggcg ctctcttctg cctatctgag
    30721 tacggaatat aaagatgtgg tctcattcgc cctgctgatt ctggtgctgc tggtgatgcc
    30781 gaccggtatt ctgggtcgcc cggaggtaga gaaagtatga aaccgatgca tattgcaatg
    30841 gcactgctct ctgccgcgat gttctttgtc ctcgctggcg tctttatggg cgtgcaactg
    30901 gagctggatg gcaccaaact ggtggtcgac agcgcttcgg atgtccgttg gcagtgggtg
    30961 tttatcggca cggcggtggt ctttttcttc cagcttttgc gacctgcttt ccagaaaggg
    31021 ctaaaaagcg tttccgggcc gaagtttatt ctgcccgcga ttgatggctc cacggtgaag
    31081 cagaaactgt tcctcgtggc gctgctggtg cttgcggtgt cgtggccgtt tatggtgtca
    31141 cgtgggacgg tggatatcgc cactctgacc atgatctaca ttatcctcgg tctcgggctg
    31201 aatgtggttg ttggtctttc tggtctgctg gtgctggggt acggcggttt ttacgccatc
    31261 ggcgcttaca cttttgcgct gctcaatcac tattacggtc tggggttctg gacctgtttg
    31321 ccgattgccg gactgatggc agcggcggcg ggcttcctgc ttggttttcc ggtgttgcgt
    31381 ctgcgcggtg attatctggc gatcgttacc ctcggtttcg gcgaaattgt gcgcatattg
    31441 ctgctcaata acaccgaaat taccggcggc ccgaacggta tcagtcagat cccgaaaccg
    31501 actttcttcg gccttgagtt cagccgtacc gcccgtgaag gcggttggga cacgttcagt
    31561 aatttctttg gcctgaaata cgatccctcc gatcgcgtca tcttcctgta tctggtggcg
    31621 ttgctgctgg tggtgctaag cctgtttgtc attaaccgcc tgctgcggat gccgctgggg
    31681 cgtgcgtggg aagcgttgcg tgaagatgag atcgcctgcc gttcgctggg cttaagcccg
    31741 cgtcgtatca agctgactgc ctttaccatc agtgccgcgt ttgccggttt tgccggaacg
    31801 ctgtttgctg cgcgccaggg ctttgtcagc ccggaatcct tcacctttgc tgaatcggcg
    31861 tttgtactgg cgatagtggt gctcggcggt atgggatcgc agtttgcggt gattctggcg
    31921 gcaattttgc tggtggtatc gcgcgaattg atgcgtgatt tcaacgaata cagcatgtta
    31981 atgctcggtg gtttgatggt gctgatgatg atctggcgtc cgcagggctt gctgcccatg
    32041 acgcgcccgc aactgaagct gaaaaacggc gcagcgaaag gagagcaggc atgagtcagc
    32101 cattattatc tgtgaacggc ctgatgatgc gcttcggcgg cctgctggcg gtgaacaacg
    32161 tcaatcttga actgtacccg caggagatcg tctcgttaat cggccctaac ggtgccggaa
    32221 aaaccacggt ttttaactgt ctgaccggat tctacaaacc caccggcggc accattttac
    32281 tgcgcaatca gcacctggaa ggtttaccgg ggcagcaaat tgcccgcatg ggcgtggtgc
    32341 gcaccttcca gcatgtgcgt ctgttccgtg aaatgacggt aattgaaaac ctgctggtgg
    32401 cgcagcatca acaactgaaa accgggctgt tctctggcct gttgaaaacg ccttctttcc
    32461 gtcgcgccca gagcgaagcg ctcgaccgcg ccgccacctg gcttgagcgc attggtttgc
    32521 tggaacacgc caaccgtcag gcgagtaacc tggcctatgg tgaccaacgc cgtctggaga
    32581 ttgcccgctg catggtgacg cagccggaga ttttaatgct cgacgaaccg gcggcaggtc
    32641 ttaacccaaa agaaaccaaa gagctggatg agctgattgc cgaactgcgc aatcatcaca
    32701 acaccactat cttgttgatt gaacacgata tgaagctggt gatggggatt tcagaccgaa
    32761 tttatgtggt caatcagggg acgccactgg caaacggtac gccggagcaa atccgtaata
    32821 acccggacgt gatccgtgcc tatttaggtg aggcataaga tggaaaaagt catgttgtcc
    32881 tttgacaaag tcagcgccca ctacggcaaa atccaggcgc tgcatgaggt gagcctgcat
    32941 atcaatcagg gcgagattgt caccctcatt ggcgcgaacg gggcggggaa aaccaccttg
    33001 ctcggcacgt tatgcggcga tccgcgcgct accagcgggc gaattgtgtt tgatgataaa
    33061 gacattaccg actggcagac ggcgaaaatc atgcgcgaag cggtagcgat tgtcccggaa
    33121 gggcgtcgcg tcttctcgcg gatgacggtg gaagagaacc tggcgatggg cggctttttt
    33181 gccgaacgcg accagttcca ggagcgcata aagtgggtgt atgaactgtt cccacgtctg
    33241 catgagcgcc gtgttcagcg ggcaggcacc atgtccggcg gtgagcaaca aatgctggcg
    33301 attggtcgcg cgctgatgag caacccgcgt ttgctgctac ttgatgagcc atcgcttggt
    33361 cttgcgccga ttatcatcca gcaaattttc gacaccatcg agcagctgcg cgagcagggg
    33421 atgactatct tcctcgtcga gcagaacgcc aaccaggcgc taaagctggc ggatcgcggc
    33481 tacgtgctgg aaaacggcca tgtggtgctc tccgatactg gtgatgcact actggcgaat
    33541 gaagcggtga gaagtgcgta tttaggcggg taataacacg ttgattgata aggagtcaaa
    33601 agactccttt gagacaggtg acaaatgtaa aattgcctga tgcgctgcgc ttatcaggcc
    33661 tactgggtga gttgcaatat attgaatttg cacgatcttg taggccggat aaggcgttta
    33721 cgccgcatcc ggcatgaaac gatgagtaat ctgtagagtt tgattcagac cttctatttt
    33781 tccgcttatc cgtgccccat ctcccatttt ccctcaccca cgccgtcacc gccttgtcat
    33841 ctttctgaca ccttacttgc aggtttatga agttggcacc gccaccatga tggcatcgaa
    33901 agccattaaa ccggtatatg acgtgtttaa agaggcaggg attcaattcg atgagtcgca
    33961 gtttgtgccg acggtttccg gttactactc cgacagcaaa acgggccact tactctccca
    34021 gccattcaac agctcgactc ccgttctcta ttacaacaaa gacgccttca agaaagcggg
    34081 gttagacccg gaacagccgc cgaaaacctg gcaggatctg gcggactctt ccgcaaaact
    34141 gaaagcctcc ggtatgaagt gcggctacgc cagcggctgg cagggctgga tccaactgga
    34201 aaactttagc gcctggaacg gtctgccgtt tgccagcaaa aacaacggct ttgacggcac
    34261 ggacgcggtg ctggagttca acaagccgga gcaggtgaaa cacattgcca tgctcgaaga
    34321 gatgaacaag aagggcgact tcagctacgt cggtcgtaag gatgaatcca ccgagaagtt
    34381 ctataacggt gattgcgcga tgaccaccgc ctcttccggt tctctcgcta atattcgtga
    34441 gtacgccaaa tttaactatg gcgtaggcat gatgccttac gacgccgatg cgaaagatgc
    34501 accgcaaaac gccattatcg gcggggccag cctgtgggtg atgcagggca aaaataaaga
    34561 aacgtatacc ggcgtggcga agttcctcga cttcctcgcg aagccagaaa acgctgccga
    34621 gtggcatcag aaaaccggtt atctgccaat caccaaagca gcgtatgacc tgacccgtga
    34681 gcagggcttt tatgagaaaa acccaggggc ggataccgcg acgcgtcaga tgctgaataa
    34741 gccgccgttg ccgttcacca aagggctgcg tctgggcaac atgccgcaga tccgcgtgat
    34801 tgtggatgaa gagctggaga gcgtgtggac cggtaagaag acaccacagc aggcactgga
    34861 taccgccgtt gagcgtggaa atcagttgct gcgccgcttt gagaaatcga cgaagtctta
    34921 atcagtgtaa tgtcggatgc gtttcgctta tctgacctgg catcgcgtgt aggccggata
    34981 agcgaagcgc atccggcaca gttcaggaat taaccgtaat gtcatcatcc cgtccggtgt
    35041 tccgctcgcg ctggctgcct tatctgctgg tcgcgccgca gctcatcatc accgttatct
    35101 tttttatctg gcctgcgggc gaagcgttgt ggtactcgcg gcaaagcgtc gatccgtttg
    35161 gtttctccag ccagtttgtc ggtctggata acttcgtcac gctgttccat gacagctact
    35221 atctagactc cttctggacg acgataaaat tcagcacctt tgtcaccgtc agcggtttgc
    35281 tggtatcgct gttctttgcg gcgctggtgg agtacatcgt gcgcggcagc cgtttctatc
    35341 aaaccttgat gctgctgccg tatgccgtgg ctcccgccgt tgccgccgta ttgtggatct
    35401 tcctgtttaa ccccggtcgc gggctgatca cccattttct cgctgagttc ggctacgact
    35461 ggaaccacgc gcaaaacagc ggtcaggcaa tgtttctggt ggtgtttgcc tcggtgtgga
    35521 agcaaatcag ctacaacttt ctgttcttct atgccgcgct gcactccatt ccccgttcgt
    35581 tgattgaagc cgcagctatt gacggcgcag ggccgattcg ccgcttcttt aagattgctc
    35641 tgccgcttat cgctccggtg agtttcttcc tgctggtggt gaacctggtg tacgccttct
    35701 tcgacacctt cccggtgatc gacgccgcca cgtccggcgg gccggttcag gccaccacta
    35761 cgctgattta taagatctac cgcgaaggct ttaccggact ggatctggct tcgtctgccg
    35821 cgcagtcggt ggtgttgatg ttcctcgtca tcgtgctgac ggtggtgcag ttccgctatg
    35881 ttgaaagcaa ggtgcgttac caatgattga gaaccgtccg tggctgacga tattcagcca
    35941 taccatgctg atcctcggga tcgcggtgat cctcttcccg ctgtacgtgg cgtttgtcgc
    36001 ggcgacgctg gataaacagg ccgtctatgc cgcgccgatg acgctcatcc ccggcacaca
    36061 tctgctggaa aatatccaca acatctgggt taacggggta ggcacgaata gcgcgccgtt
    36121 ctggcggatg ttgcttaaca gctttgtgat ggcgttcagc attacgctcg gcaaaattac
    36181 cgtctcgatg ctctcggcat ttgccattgt ctggtttcgt tttccgctac gtaacctctt
    36241 cttctggatg atttttatca ccctgatgct gccggttgaa gtacgtatct tcccgacggt
    36301 ggaagtcatc gccaacctga aaatgctcga cagctacgcc ggtttaacgc tgccgctgat
    36361 ggcctcggcg accgccacgt ttctgttccg ccagttcttt atgaccctac cggatgaatt
    36421 ggtggaagcc gcgcggatcg acggcgcgtc accaatgcgt ttcttttgcg acatcgtttt
    36481 tccgttgtcc aaaactaatc tggcggcgct gtttgtgatc acctttatct acggctggaa
    36541 tcagtatttg tggccgttgt tgattattac cgacgttgat ctcggcacca ccgtggcagg
    36601 gatcaaaggg atgatcgcta caggcgaagg caccacggaa tggaactcgg taatggcggc
    36661 gatgttgtta acgcttatcc ctccggtggt gattgtttta gtgatgcagc gtgccttcgt
    36721 gcgcggcctg gtcgatagtg agaaataaga tggcaggact gaaattacag gcagtaacca
    36781 aaagctggga tggcaaaacc caggtcatta aaccgctgac ccttgatgtg gcggatggcg
    36841 aatttatcgt gatggtcggg ccgtctggct gcgggaaatc gacgctgctg cgcatggttg
    36901 ccgggctgga gcgggtgaca gaaggcgata tctggatcaa cgaccagcgc gtgaccgaaa
    36961 tggagccgaa agatcgcggg attgcgatgg tgttccagaa ttacgcgctt tatccgcata
    37021 tgagcgtaga ggagaacatg gcgtgggggc tgaaaattcg cggcatgggc aagcagcaaa
    37081 ttgccgagcg cgttaaagaa gcggcgcgca ttctggaact ggacggtctg cttaagcgtc
    37141 gtccgcgtga gctatccggc ggtcagcgcc agcgtgtggc gatgggccgc gcgattgtgc
    37201 gcgatccggc ggtgttcttg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg
    37261 tgcagatgcg tcttgaactg caacagttgc accgtcgcct gaaaacgact tcactctacg
    37321 ttactcacga tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggtg
    37381 gcgttgccga acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg
    37441 tggcgagttt tatcggcagc ccggcgatga acctgctgac aggccgcgtg aataatgaag
    37501 gcacgcattt cgaactggac ggcggtatcg tgctaccgct aaacggtggc taccgtcagt
    37561 atgccgggcg taaaatgact ctcggcattc gcccggaaca tatagcgcta agctcgcagg
    37621 cagaaggtgg tgtgccgctg gtgatggaca cgctggagat cctcggcgca gataacctgg
    37681 cgcacggacg ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga
    37741 cggcaggcag cacgctgtgg ctgcatttgg cggaaaatca gctgcatctt tttgatggtg
    37801 aaacaggaca acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg
    37861 cggtaagctg gccccggaaa acaccctggc ggcaatcgac gtcggggcaa aatacggtca
    37921 taagatgatc gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga
    37981 cgacaatctc gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga
    38041 tttactgcgc gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc
    38101 gttgctttcg caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat
    38161 caaacccacc actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca
    38221 actgtgggcc ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc
    38281 tgcacaacag gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga
    38341 ctggcgcgaa ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact
    38401 cgataaagcg cgagtgatgc agttgaaaga cgccggatta cggattctgg tttataccgt
    38461 caataaaccc cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga
    38521 tgcgattgac gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc
    38581 tgcggcgtgc tgggctgatt cagcatgtcg ccgtttctcc tttccggcag catatgctgc
    38641 tgattcaacg aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc
    38701 agcatttgct gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa
    38761 ttattgttta tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc
    38821 tgattcagca tccctttttg ctggatttgc tgagtctgca tctgggtttg catccgctgc
    38881 tggctgggta tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca
    38941 aagccgacaa acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct
    39001 ctgaagatat cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat
    39061 ataccaggct tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac
    39121 gtttttacgc cgggtggcca ttgctgctct gctctcagga agttgtttta gcgccgccgc
    39181 cgcgcctcct gcgccgcccg tctcgtatgg tgtggaggaa gatgtcttcc acccggtacg
    39241 cgcgaaacag ggaatggtag cgtctgtgga cgccactgcc actcaggtgg gggtggatat
    39301 cctcaaggag ggcgggaatg ccgttgatgc cgccgtggcg gtgggctacg cgctggcggt
    39361 aacgcatccg caggcaggga atctgggcgg tggtggtttt atgttaatcc gctcgaaaaa
    39421 tggcaatacc acggctatcg atttccgcga aatggcaccc gccaaagcga cccgcgatat
    39481 gttcctcgat gatcagggca acccggacag caaaaaatca ctcacttcgc atctggcttc
    39541 cggcacaccg ggtacggtag caggtttctc gctggcgctg gataaatacg gcaccatgcc
    39601 gctgaacaaa gtcgtgcagc ccgcgtttaa actggcacgc gatggtttta tcgttaacga
    39661 cgcgctggct gacgatctca aaacctacgg tagcgaagtg ttgccgaatc acgaaaacag
    39721 taaagctatc ttctggaaag agggcgagcc gctgaaaaag ggcgacacgc tggtgcaggc
    39781 gaacctggca aagagcctgg agatgattgc tgaaaacggc ccggacgaat tctataaagg
    39841 cacgattgcg gaacagatcg cccaggagat gcagaaaaac ggtggcttga tcactaaaga
    39901 agatttagcg gcctataaag cggtcgaacg cactccgata agcggcgatt atcgcgggta
    39961 tcaggtttac tccatgccac cgccatcctc cggcgggatc catatcgtac aaatcctcaa
    40021 tattctggaa aacttcgata tgaagaaata cggctttggc agcgccgatg cgatgcaaat
    40081 catggcagaa gcggagaaat acgcctacgc cgaccgctcg gaatatcttg gcgacccgga
    40141 ttttgtcaaa gtcccgtggc aggcactgac caataaagcc tatgccaaat ccattgccga
    40201 tcaaattgat atcaacaaag cgaagccgtc cagtgagatt cgccctggca agcttgcgcc
    40261 ttatgagagt aatcaaacta cccattactc tgtagtggat aaagacggta acgcggtggc
    40321 ggtgacctat acgctgaaca ccaccttcgg tacgggcatt gtcgcgggcg agagcggtat
    40381 tctgcttaat aatcagatgg atgatttctc cgccaaaccg ggcgtaccga acatttacgg
    40441 gctggtgggc ggtgatgcca acgccgtcgg gccgaataaa cgtccgctgt catcgatgtc
    40501 accaaccatt gtggtgaaag acggtaaaac ttggctggtt accggtagtc ctggcgggag
    40561 tcggattatt accaccgtgc
//
LOCUS       DAZSBF010000007        39895 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZSBF010000007 DAZSBF010000000
VERSION     DAZSBF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.007"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(169..844)
                     /locus_tag="ACHXWD_000260"
                     /pseudo
     CDS             complement(169..844)
                     /locus_tag="ACHXWD_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(989..1597)
                     /locus_tag="ACHXWD_000261"
     CDS             complement(989..1597)
                     /locus_tag="ACHXWD_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR7619292.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1781..2098)
                     /gene="metJ"
                     /locus_tag="ACHXWD_000262"
     CDS             complement(1781..2098)
                     /gene="metJ"
                     /locus_tag="ACHXWD_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR7619293.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2375..3535
                     /gene="metB"
                     /locus_tag="ACHXWD_000263"
     CDS             2375..3535
                     /gene="metB"
                     /locus_tag="ACHXWD_000263"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR7619294.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3538..5970
                     /gene="metL"
                     /locus_tag="ACHXWD_000264"
     CDS             3538..5970
                     /gene="metL"
                     /locus_tag="ACHXWD_000264"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR7619295.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6109..6243)
                     /locus_tag="ACHXWD_000265"
     CDS             complement(6109..6243)
                     /locus_tag="ACHXWD_000265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619296.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6319..7209
                     /gene="metF"
                     /locus_tag="ACHXWD_000266"
     CDS             6319..7209
                     /gene="metF"
                     /locus_tag="ACHXWD_000266"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR7619297.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7538..9718
                     /gene="katG"
                     /locus_tag="ACHXWD_000267"
     CDS             7538..9718
                     /gene="katG"
                     /locus_tag="ACHXWD_000267"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR7619298.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9812..10717
                     /gene="yijE"
                     /locus_tag="ACHXWD_000268"
     CDS             9812..10717
                     /gene="yijE"
                     /locus_tag="ACHXWD_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR7619299.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10744..11361)
                     /locus_tag="ACHXWD_000269"
     CDS             complement(10744..11361)
                     /locus_tag="ACHXWD_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR7619300.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11635..12738)
                     /gene="gldA"
                     /locus_tag="ACHXWD_000270"
     CDS             complement(11635..12738)
                     /gene="gldA"
                     /locus_tag="ACHXWD_000270"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR7619301.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12749..13411)
                     /gene="fsa"
                     /locus_tag="ACHXWD_000271"
     CDS             complement(12749..13411)
                     /gene="fsa"
                     /locus_tag="ACHXWD_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR7619302.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13423..15924)
                     /gene="ptsP"
                     /locus_tag="ACHXWD_000272"
     CDS             complement(13423..15924)
                     /gene="ptsP"
                     /locus_tag="ACHXWD_000272"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR7619303.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16233..17312
                     /locus_tag="ACHXWD_000273"
     CDS             16233..17312
                     /locus_tag="ACHXWD_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR7619304.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTGGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17327..17647
                     /locus_tag="ACHXWD_000274"
                     /pseudo
     CDS             17327..17647
                     /locus_tag="ACHXWD_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17698..19994
                     /locus_tag="ACHXWD_000275"
                     /pseudo
     CDS             17698..19994
                     /locus_tag="ACHXWD_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19960..20838
                     /locus_tag="ACHXWD_000276"
     CDS             19960..20838
                     /locus_tag="ACHXWD_000276"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR7619305.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20840..21181
                     /locus_tag="ACHXWD_000277"
     CDS             20840..21181
                     /locus_tag="ACHXWD_000277"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR7619306.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21168..22019)
                     /locus_tag="ACHXWD_000278"
     CDS             complement(21168..22019)
                     /locus_tag="ACHXWD_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7619307.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22234..23967)
                     /locus_tag="ACHXWD_000279"
     CDS             complement(22234..23967)
                     /locus_tag="ACHXWD_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR7619308.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24150..26801)
                     /gene="ppc"
                     /locus_tag="ACHXWD_000280"
     CDS             complement(24150..26801)
                     /gene="ppc"
                     /locus_tag="ACHXWD_000280"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR7619309.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27153..28304)
                     /gene="argE"
                     /locus_tag="ACHXWD_000281"
     CDS             complement(27153..28304)
                     /gene="argE"
                     /locus_tag="ACHXWD_000281"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR7619310.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28458..29462
                     /gene="argC"
                     /locus_tag="ACHXWD_000282"
     CDS             28458..29462
                     /gene="argC"
                     /locus_tag="ACHXWD_000282"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR7619311.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29470..30246
                     /gene="argB"
                     /locus_tag="ACHXWD_000283"
     CDS             29470..30246
                     /gene="argB"
                     /locus_tag="ACHXWD_000283"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR7619312.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30307..31680
                     /gene="argH"
                     /locus_tag="ACHXWD_000284"
     CDS             30307..31680
                     /gene="argH"
                     /locus_tag="ACHXWD_000284"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR7619313.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31947..32864
                     /gene="oxyR"
                     /locus_tag="ACHXWD_000285"
     CDS             31947..32864
                     /gene="oxyR"
                     /locus_tag="ACHXWD_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR7619314.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPIADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32847..34247)
                     /gene="sthA"
                     /locus_tag="ACHXWD_000286"
     CDS             complement(32847..34247)
                     /gene="sthA"
                     /locus_tag="ACHXWD_000286"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR7619315.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34568..35218
                     /gene="fabR"
                     /locus_tag="ACHXWD_000287"
     CDS             34568..35218
                     /gene="fabR"
                     /locus_tag="ACHXWD_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR7619316.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35218..35577
                     /locus_tag="ACHXWD_000288"
     CDS             35218..35577
                     /locus_tag="ACHXWD_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR7619317.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35617..36717)
                     /gene="trmA"
                     /locus_tag="ACHXWD_000289"
     CDS             complement(35617..36717)
                     /gene="trmA"
                     /locus_tag="ACHXWD_000289"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR7619318.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36831..37021
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37086..38930
                     /gene="btuB"
                     /locus_tag="ACHXWD_000290"
     CDS             37086..38930
                     /gene="btuB"
                     /locus_tag="ACHXWD_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR7619319.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38875..39732
                     /gene="murI"
                     /locus_tag="ACHXWD_000291"
     CDS             38875..39732
                     /gene="murI"
                     /locus_tag="ACHXWD_000291"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR7619320.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9105 a        10029 c        11011 g         9750 t
ORIGIN      
        1 gggcggggga atgtcatatg cctatgaata tgatgtaaaa tataatatat gtcatttgag
       61 aaaaaagagc agaatgaggt aaaaatcttt ggctgtgtat tcagtctggt aatcatccaa
      121 ggaaagctta tcagaagggt gatgactcca attagtagaa catatatttt cgataaaagc
      181 accgatgact ttttcatgaa gttctacaga acgcgagaaa cagatggttc tgcgggccag
      241 acgtttgatt cgggttctca gggtcagatt gttacgctca atgtgctgag taaaaatctt
      301 accagtcaga tgcttctcct tcggtaactc tctagcgtaa ctaccccatt cgtcagtggt
      361 caacatgccg atggtaaaag ggtaagcagc gcccgcaatt cacgacaggt ttcgtcagtc
      421 cgggggccaa aagtgtaggc cagaacacca ccagttttcg tgttgtacac gtaccagagc
      481 cagtgctgtc gcgctttact gccgacaaag ctccattgct gactaagttt gcagatgagt
      541 gccacatcag catgagcgac cggggatgac gttattcttc gtggcgagag ttttttaaag
      601 ttcggatgac tgtattaatg ccgattttca gcgtacgggc agtatcgcga accccggcac
      661 cgttgaaagt catttcagcg atttggtctt taactcccgg tttacggggc tcgtaacagt
      721 aactgagctg aaatacccgg tgacagtcac ggccgcggaa tcgctcatgt ctttttgggt
      781 tctgcccgtg acggtaaacc tgaacggact ggcaacgggg gcaatgaagg ttaactgtga
      841 ccatgaaaca accttaaaag cgagtattat atccttaatt caactaattg gaggcatcac
      901 cgtttaccgg agcgacagct tgcggaaagg tcgtgcaact acaaagcaac aaacagacta
      961 cggcgggaat tatttttttc atcatccatt accacgaaaa cagcggccac tctttggcga
     1021 ctgtggtgag ttgtggcgcg tcgaaaacgg cttgtggtga aactaccgtt tcctcaagcg
     1081 gaatattttt gccgtagcgt tctttgagct tagcctggac cagcgggttg ctgagatcga
     1141 actctttcaa cttctgctgc tcgcccacgc gcattcccag cgcgttctgg taaaccttcc
     1201 acaccacttc cgaacagtac tggcgatcgt cgctccatga gaagctgaaa tcatacggtt
     1261 taccgagata acgttttgcc gtttgcgcca gtttttgctg ttgttcaaca ctcagtccgc
     1321 cttcaacgcg gcgaacaacg tatttgccct tttcaccatg ggcgatccac tgcttgagcg
     1381 gggggtattt caccgggccg accgcttcaa aaacgtaggg ctttttgttg cgtatcacca
     1441 gcataccggt gtggctataa tcggaatggg tcgccagttg gatcgcttta ctttgcgatg
     1501 agcgagagat ctgaaagatg atgtcgccgg tttgtggctg ccaggcaaag gcaggtacag
     1561 aaaccagcag gctgaggatc agcagcctgt ttttcatagt taaacgtcca tgtataaaaa
     1621 gcggtgggtc gcagacaacg tgctcgttgt ttatgccgga tgcggcgtga acgccttatc
     1681 cggcctacaa gttcgtgcaa attcaataaa ttgcaatatg acgtaggcct gataagcgta
     1741 gcgcatcagg cgattccact ccgcgccgct cttttttgct ttagtattcc cacgtctccg
     1801 ggttaatccc catctcacgc atgatctctt ttgccgcttc cgggatttcg tcgctgcgct
     1861 ctttacgcag atcggcatca tccggcaaag gttgcccggt aaaggcatgc agaaacgctt
     1921 cgcacagcag ctcgctgttg gtagcgtgac gcaggttgtt cacctgacga cgcgtgcgtt
     1981 catcggtgag gatttttaac accttaagag gaatggaaac cgtaatcttt ttgacttgtt
     2041 cactcttctt gccgtgctca gcgtatgggc tgatatattc gccgctccat tcagccatga
     2101 gatacttaat cctcttcgtc aataaattga gaccagacca cacaatttat gtgggtactg
     2161 accgtaaacc cgcatagttt accgtacagg cgttaccgtg acatcgtgta atgcacctgt
     2221 cggcgtgata atgcatataa ttttaacggc tatttgggat ttgctcaatc tatacgcaaa
     2281 gaagtttaga tgtccagatg tattgacgtc cattaacaca atgtttactc tggtgcctga
     2341 catttcaccg acaaagccca ggaaacttca tcacatgacg cgtaaacagg ccaccatcgc
     2401 agtgcgtagc gggttaaatg acgacgaaca gtatggttgc gttgtccctc ccatccatct
     2461 ttccagcacc tataacttta ccggatttaa tgaaccgcgc gcgcatgatt actcgcgtcg
     2521 cggcaaccca acgcgcgatg tggttcagcg tgcgctggca gaactggaag gcggtgctgg
     2581 cgcagtactt actaataccg gcatgtccgc gattcacctg gtaacgaccg tctttttgaa
     2641 acctggcgat ctgctggttg cgccgcacga ctgctacggc ggtagctatc gcctgttcga
     2701 cagtctggcg aaacgcggtt gctatcgcgt gttgtttgtt gatcaaggcg atgaacaggc
     2761 attacgggca gcgctggcag aaaaacccaa actggtactg gtagaaagcc caagtaatcc
     2821 attgttacgc gtcgtggata ttgcgaaaat ctgccatctg gcaagggaag tcggggcggt
     2881 gagcgtggtg gataacacct tcttaagccc ggcattacaa aatccgctgg cattaggtgc
     2941 cgatctggtg ttgcattcat gcacgaaata tctgaacggt cactctgacg tggtggctgg
     3001 cgtggtgatt gctaaagacc cggacgttgt cactgaactt gcctggtggg caaacaatat
     3061 tggcgtgacg ggcggcgcgt ttgacagcta tctgctgcta cgcgggttgc gaacgctggt
     3121 gccgcgtatg gagctggcgc agcgcaacgc gcaggcgatt gtgaaatatc tgcaaaccca
     3181 gccgttggtg aaaaaactgt atcacccgtc gctgccggaa aatcaggggc atgaaattgc
     3241 cgcgcgtcag caaaaaggct ttggcgcaat gttgagtttt gaactggatg gcgatgagca
     3301 gacgctgcgt cgtttcctga gcgggctgtc gttgtttacg ctggcggaat cattaggggg
     3361 agtggaaagt ttaatctctc acgccgcaac catgacacat gcaggcatgg caccagaagc
     3421 gcgtgctgcc gccgggatct ccgagacgct gctgcgtatc tccaccggta ttgaagatgg
     3481 cgaagattta attgccgacc tggaaaatgg cttccgggct gcaaacaagg ggtaaaaatg
     3541 agtgtgattg cgcaggcagg ggcgaaaggt cgtcagctgc ataaatttgg tggcagtagt
     3601 ctggctgatg tgaagtgtta tttgcgtgtc gcgggcatta tggcggagta ctctcagcct
     3661 gacgatatga tggtggtttc cgccgccggt agcaccacta accagttgat taactggttg
     3721 aaactaagcc agaccgatcg tctctctgcg catcaggttc aacaaacgct gcgtcgctat
     3781 cagtgcgatc tgattagcgg tctgctaccc gctgaagaag ccgatagcct cattagcgct
     3841 tttgtcagcg atctggaacg cctggcggcg ctgctcgaca gcggtattaa cgacgcagtg
     3901 tatgcggaag tggtgggcca cggggaagta tggtcggcac gcctgatgtc tgcggtactt
     3961 aatcaacaag ggctgccagc ggcctggctt gatgcccgcg agtttttacg cgctgaacgc
     4021 gccgcacaac cgcaggttga tgaagggctt tcttacccgt tgctgcaaca gctactggtg
     4081 caacatccgg gcaaacgtct ggtggtgacc gggtttatca gccgtaacaa cgccggtgaa
     4141 acggtgctgc tggggcgtaa cggttccgac tattccgcga cacaaatcgg tgcgctggcg
     4201 ggtgtttctc gtgtaaccat ctggagcgac gtcgccgggg tatacagtgc cgacccgcgt
     4261 aaagtgaaag atgcctgtct gctgccgttg ctgcgtctgg atgaagccag cgaactggct
     4321 cgtctggcgg ctcccgttct tcacgcccgt actttacagc cggtttctgg cagcgaaatc
     4381 gacctgcaac tgcgctgtag ctacacgccg gatcaaggtt ccacgcgcat tgaacgcgtg
     4441 ctggcctccg gtactggtgc gcgtattgtc accagccacg atgatgtctg tttgattgag
     4501 tttcaggtgc ccgccagtca ggatttcaaa ctggcgcata aagagatcga ccaaatcctg
     4561 aaacgcgcgc aggtacgccc gctggcggtt ggcgtacata acgatcgcca gttgctgcaa
     4621 ttttgctaca cctcagaagt ggccgacagt gcgctgaaaa tcctcgacga agcggggtta
     4681 cctggcgaac tgcgcctgcg tcaggggctg gcgctggtgg cgatggtcgg tgcaggcgtc
     4741 acccgtaacc cgctgcattg ccaccgcttc tggcagcaac tgaaaggcca gccggtcgaa
     4801 tttacctggc agtccgatga cggcatcagc ctggtggcag tactgcgcac cggcccgacc
     4861 gaaagcctga ttcaggggct gcatcagtcc gtcttccgcg cagaaaaacg catcggcctg
     4921 gtattgttcg gtaagggcaa tatcggttcc cgttggctgg aactgttcgc ccgtgagcag
     4981 agcacgcttt cggcacgtac cgactttgaa tttgtgctgg caggcgtggt ggacagccgc
     5041 cgcagtctgt tgagctatga cgggctggac gccagccgcg cgttagcctt cttcaacgat
     5101 gaagcggttg agcaggatga agagtcgttg ttcctgtgga tgcgcgccca tccgtatgat
     5161 gatttagtgg tgctggacgt taccgccagc cagcagcttg ctgatcagta tcttgatttc
     5221 gccagccacg gtttccacgt catcagcgcc aacaaactgg cgggagccag cgacagcaat
     5281 aaatatcgcc agatccacga cgccttcgaa aaaaccgggc gtcactggct gtacaatgcc
     5341 accgtcggtg cgggcttacc gatcaaccac accgtgcgcg atctgatcga cagcggcgat
     5401 actattttgt cgatcagcgg gatcttctcc ggcacgctct cctggttgtt cctgcaattc
     5461 gacggtagcg tgccgtttac cgagctggtg gatcaggcgt ggcagcaggg cttaaccgaa
     5521 cctgacccgc gtgacgatct ctctggcaaa gacgtgatgc gcaagctggt gattctggcg
     5581 cgtgaagcag gttacaacat cgaaccggat caggtacgcg tggagtcgct ggtgcctgcc
     5641 cattgcgaag gcggcagcat cgaccatttc tttgaaaatg gcgatgaact taacgagcag
     5701 atggtgcaac ggctggaagc ggcccgcgaa atggggctgg tgctgcgcta cgtggcgcgt
     5761 ttcgatgcca atggcaaagc gcgtgtgggc gtggaagcgg tgcgtgaaga ccatccgctg
     5821 gcatcactgc tgccgtgcga taacgtcttt gccatcgaaa gccgctggta tcgcgataat
     5881 ccgctggtga tccgtgggcc tggcgctggg cgcgacgtca ccgccggggc gattcagtcg
     5941 gatatcaacc ggctggcaca gttgttgtaa tttcagaaat ttaataatgc ccggtactca
     6001 tgttttcggg tttatggttt ctaatgaaat atattgaatt atcataggat taggccggat
     6061 taagcgttta cgacgaatcc ggcaagaagc aataagtaca tggttagttt atatttgcag
     6121 tccggtttgc tttgcatacc ggattttctt tttcttacca tcctgaagtt ttttcatctt
     6181 ccctgatttt tcctcaccat cattggtcat ttttcggttg acgcccttcg gcttttcctt
     6241 catctttaca tctggacgtc taaacggata gatgtgcaca acacaacata taactacaag
     6301 cgattgatga ggtaaggtat gagctttttt cacgccagcc agcgggatgc cctgaatcag
     6361 agcctggcag aagtccaggg gcagattaac gtttcgttcg agtttttccc gccgcgtacc
     6421 agtgaaatgg agcagaccct gtggaactcc atcgatcgcc ttagcagcct gaaaccgaag
     6481 tttgtatcgg tgacctatgg cgcgaactcc ggcgagcgcg accgtacgca cagcattatt
     6541 aaaggcatta aagatcgcac tggtctggaa gcggcaccgc atcttacttg cattgatgcg
     6601 acgcccgacg agttgcgcac cattgcacgc gactactgga ataacggtat tcgtcatatc
     6661 gtggcgctgc gtggcgatct gccgccggga agtggtaagc cagaaatgta tgcttctgac
     6721 ctggtgacgc tattaaaaga agtggcagat ttcgatatct ccgtggcggc gtatccggaa
     6781 gttcacccgg aagcaaaaag cgctcaggcg gatttgctta atctgaaacg caaagtcgat
     6841 gccggagcca accgcgcgat tactcagttc ttcttcgatg tcgaaagcta cctgcgtttt
     6901 cgtgaccgct gtgtatcggc gggcattgat gtggaaatta ttccgggaat tttgccggta
     6961 tctaacttta aacaggcgaa gaaatttgcc gatatgacca acgtgcgtat tccggcgtgg
     7021 atggcgcaaa tgttcgacgg cctggatgat gatgccgaaa cccgcaaact ggtgggcgcg
     7081 aatattgcca tggatatggt gaagatttta agccgtgaag gagtgaaaga tttccacttc
     7141 tatacgctta accgcgctga aatgagttac gcgatttgcc atacgctggg tgttcgacct
     7201 ggtttataaa tagtgtggct tttgtgaaaa tcacacagtg atcacaaatt ttaaacagag
     7261 cacaaaatgc tgcctcgaaa tgagggcggg aaaataaggt tgtcagcctt gttttctccc
     7321 tcattacttg aaggatatga agctaaaacc cttttttata aagcatttgt ccgaattcgg
     7381 acataatcaa aaaagcttaa ttacgatcaa tttgacctac atctctttaa ccaacaatat
     7441 gtaagatctc aactatcgca tccgtggatt aattcaatta taacttctct ctaacgctgt
     7501 gtatcgtaac ggtaacactg tagaggggag cacattgatg agcacgtcag acgatatcca
     7561 taacacgaca gccactggca aatgcccgtt ccatcagggc ggtcacgacc agagcgcggg
     7621 ggcgggcaca accactcgcg actggtggcc aaatcaactc cgtgttgacc tgttaaacca
     7681 acattctaat cgttctaacc cgctgggtga ggactttgac taccgcaaag aattcagcaa
     7741 attagattac tacggcctga aaaaagatct gaaagccctg ctgacagaat ctcagccgtg
     7801 gtggccagcc gactggggca gctacgccgg tctgtttatt cgtatggcct ggcacggcgc
     7861 aggtacttac cgttcaatcg atggacgcgg cggcgcgggt cgtggtcagc aacgttttgc
     7921 accgctgaac tcctggccgg ataacgtaag cctcgataaa gcgcgtcgcc tgttgtggcc
     7981 aatcaaacag aaatatggtc agaaaatctc ctgggccgac ctgtttatcc tcgcaggtaa
     8041 cgtggcgctg gaaaactccg gcttccgtac cttcggtttt ggtgccggtc gtgaagacgt
     8101 ctgggaaccg gatctggatg ttaactgggg tgatgaaaaa gcctggctga ctcaccgtca
     8161 tccggaagcg ctggcgaaag caccgctggg tgcaaccgag atgggcctga tttacgttaa
     8221 cccggaaggc ccggatcaca gcggcgaacc gctttctgcg gcagcagcta tccgcgcgac
     8281 cttcggcaac atgggcatga acgacgaaga aaccgtggcg ctgattgcgg gtggtcatac
     8341 gctgggtaaa acccacggtg ccggtccgac atcaaacgta ggtcctgatc cagaagctgc
     8401 accgattgaa gaacaaggtt taggctgggc gagcacttac ggcagcggcg ttggcgcaga
     8461 tgccattacc tctggtctgg aagttgtctg gacccagacg ccgacccagt ggagcaacta
     8521 tttcttcgaa aacctgttca aatatgagtg ggtacagacc cgcagtccgg ctggcgcaat
     8581 ccagttcgaa gcggtagacg caccggaaat tatcccggat ccattcgatc cgtcgaagaa
     8641 acgtaaaccg acgatgctgg tgaccgacct gacgctgcgt tttgatcctg agttcgagaa
     8701 gatctctcgt cgtttcctca acgatccgca ggcgttcaac gaagcctttg cccgtgcctg
     8761 gttcaaactg acgcacaggg atatggggcc gaaatctcgc tacatcgggc cggaagtgcc
     8821 gaaagaagat ctgatctggc aagatccgct gccgcagccg atctacaacc cgaccgagca
     8881 ggacattatc gatctgaaat tcgcgattgc ggattctggt ctgtctgtta gtgagcgggt
     8941 atcggtggcc tgggcatctg cttctacctt ccgtggtggc gacaaacgcg gtggtgccaa
     9001 cggtgcgcgt ctggcattaa tgccgcagcg cgactgggat gtgaacgccg cagccgttcg
     9061 tgctctgcct gttctggaga aaatccagaa agagtctggt aaagcctcgc tggcggatat
     9121 catcgtgctg gctggtgtgg ttggtgttga gaaagccgca agcgccgcag gtttgagcat
     9181 tcatgtaccg tttgcgccgg gccgcgttga tgcgcgtcag gatcagactg acattgagat
     9241 gttcgagctg ctggagccaa ttgctgacgg tttccgtaac tatcgcgctc gtctggatgt
     9301 ttccaccacc gagtcactgc tgatcgacaa agcccagcaa ctgaccctga ccgcgccgga
     9361 aatgaccgcg ctggtgggcg ggatgcgcgt actgggtgcc aacttcgatg gcagcaaaaa
     9421 cggcgtcttc actgaccgcg ttggcgtatt gagcaatgac ttcttcgtga acttgctgga
     9481 tatgcgttac gagtggaaag cgaccgacga atcgaaagag ctgtttgaag gccgtgaccg
     9541 cgaaaccggc gaagtgaaat acactgccag ccgtgcggat ctggtatttg gttctaactc
     9601 tgtcctgcgt gcggtggctg aagtttacgc cagcagcgat gctcacgaga agtttgttaa
     9661 agacttcgtg gcggcatggg tgaaagtgat gaacctcgac cgtttcgacc tgctgtaatc
     9721 tgaccttctt cagcgactgc ctttcaggca gtcgctgaag tttctttacc ggcgtatagt
     9781 gtccacagga aaactacaca ctggatctct catgtctgcc gcaggaaaga gcaacccact
     9841 ggcaatcagt ggcctggttg tgctcacact tatctggagt tatagctgga ttttcatgaa
     9901 gcaagtcacc agctacatcg gtgccttcga ctttaccgcc ttacgctgca ttttcggcgc
     9961 tctcgtttta ttcatcgtcc ttttattacg tggtcgcgga atgcgcccga caccgtttaa
    10021 atacacctta gccattgccc tgttacaaac ctgcgggatg gttggcctgg cgcagtgggc
    10081 gctggtcagc ggcggagcgg ggaaggtggc gatcctaagc tataccatgc cgttctgggt
    10141 ggtgattttc gccgcgttgt ttctcggtga acgcctgcga cgtgggcaat atttcgcgat
    10201 actgattgcc gctttcggct tatttttggt gttgcagccg tggcaactcg atttctcttc
    10261 gatgaaaagt gccatgctgg caatcctctc cggcgtcagt tggggggcga gcgcgattgt
    10321 tgctaaacgt ctgtatgccc gtcatccgcg cgtggattta ttgtcgttaa catcctggca
    10381 gatgctctat gcggcgctgg tgatgagtgt ggtcgcttta ctggtgccgc aacgtgaaat
    10441 tgactggcag cccaccgtgt tctgggcgct ggcctacagt gcgattctgg cgacggcact
    10501 ggcgtggagc ttatggttgt ttgtattgaa aaacttgcct gccagtattg ccagcttaag
    10561 cacactggcc gttcccgttt gcggcgtact cttttcctgg tggctgctcg gcgagaatcc
    10621 gggggccgtt gaaggtagcg gtattgtgct gattgtgatg gcactggcgc tggtgagccg
    10681 taagaaaaaa gaagccgtta gtgtaaaaag gatctgaatt ttttcttcat gtggggcgat
    10741 cgcttattta acaaaataac gataatgccc caccatgcgc cagctaaaca gcacatcttc
    10801 ttcctgcgca cctgcgccaa tgttatgtat caccagcggc gtaccgtcgc gggcgaagcc
    10861 atctgaaacc accccaatat gtgccagccc gttatctaat cgccaggaga caatatcgcc
    10921 cgcttgatag tcactggggt tcttgctgat ggggcgtgtt ttatcgtggt gggtaaacca
    10981 ggtttccaga ttaggcaccc gacggtgatc gatgttgctg tccgggcgct ttaactgcca
    11041 tttttgcggg tactcggcaa aattcttcgc catatcttcg tgcaccagtt tctgcaaatc
    11101 gaccttctgg ctgcgcaatg cacggatcac cacatcggaa cataccccgc gtttttgcgg
    11161 gacatcaccg ccaggatagg taagctgcat atacgccgga tcgtaaaata gcgtgctgcc
    11221 gatttgctgt ctggcaccgt ctgcgatggc aaggttggta ttggcctgga tttgtaccac
    11281 ggttggtgga acggcgggag attttaagga gtggctggta aatgccgtta gcaggctgag
    11341 cagcgccagt gaagctttca tctggttcgt ccgtgaaaat aagagtcaca gattcgacct
    11401 tccgggataa cgattgttct gtagagaaaa tgtccagaag cggataaaaa actcctgaac
    11461 aacgtgggag tttcagatta ctggcacaga gtgccggatg cggcgtgaac gccttatctg
    11521 tcctaaaaaa aaagaaaatt caatatattg cagagattgc gtaggcctga taagcgtagc
    11581 gcatcaggca attttgcgtt caaactcccg gacaagccgg gagtttggag taggttattc
    11641 ccactcttgc aggaaacgct gaccgtactg gtcggctacc agcagagcgg cgtaaacctg
    11701 atctggcgtc gcgccgccag gcatgttgtg gatggtttca cattctgcac atgccgcttc
    11761 agccacaatt cgcattttcg ccgggacatc ttctttaata tccagttgag cgagagttat
    11821 tggcaaacct accgcatggc taagtgcagc tacggtttcg atttcctcaa ccggtgcgtt
    11881 ttccagaacc agctgcgtca gcgtaccaaa tgccactttt tcaccgtgat aatagtgatg
    11941 cgcgtccggg atagcggtca ggccgttatg cactgcgtgc gccgcagcca gaccaccact
    12001 ttcaaaacca acaccgctca aataggtgtt cgcttcaatc acgcgctcca gcgccggagt
    12061 cactacatgc tgttcggcag caagcatcgc tttttcgcct tcttccagca gggtgttgta
    12121 gcacagttca gccagtgcca gcgcagcctg ggtgcacttg ccgcccgcca tggtggtcgc
    12181 gccgctacga gagcaggcac gcgcttcaaa ccaggttgcc agcgcatcgc cgatacccgc
    12241 cgctaacaga cgtgcaggtg cgccagcgac gattttggtg tcgacaatga ccatattcgg
    12301 gttatttggc aacagcagat agcggtcaaa ctcaccctca tcggtgtaga taacagacaa
    12361 tgcgctgcac ggtgcatcgg tagaggcgat agtcggtgcg atcgctaccg gaacacccat
    12421 gaaatgtgcc agtgctttgg ctgtatcgag ggtttttccg ccaccgatac cgagaattgc
    12481 gccacactgc gcagtctccg cgatgccacg cagacggtcg atctcatttt gcgaacatcc
    12541 accgccaaac ggcgcaattt ctactaccag tccagcatct ttaaagcttt tctcgacagt
    12601 ggattgagca aaacctaaaa caaatttgtc acccaccact aaccagcgtt ctgccagcgg
    12661 cttcaggtat tcgcccagac gattaatcac atcagcgccc tggatgtatt tacccggtga
    12721 ttgaataatg cggtccataa ttgctccttt agagatgagt agtgccaaat gcggcattcc
    12781 agtcgtgttc gaacttctct atagctgact ctaccgcagg ggtgttgagc atttgttgcg
    12841 ctacatctaa gggcagggtg atggattcac atcctgccag taaacagtcc agcgcctgac
    12901 gcggcgtttt aaagctggct gccagcacca tgctttctgg cgcgtgcatt tctaacagcg
    12961 cttgcagctc ctgaaccgta cgaatgccgt ctccgccctg ggcatctacg cggttaacat
    13021 acggagcaac gtattttgcc cctgccagcg cggccagtaa cccttgtgca gcactgtaca
    13081 ctgcggtgcc aagcgtagta ataccctctt ttttcagtat tttaattgct gccagacctt
    13141 cggaagtcac cgggattttc accacaatac ccggaatagc gtcgcgcagg cgcttcgctt
    13201 cttccaccat cccctgcgcg tcgcggctca tggtctgagc aaacagaatg ccctcatcac
    13261 caatcgcttt ttgcagacgc ggcagcactt cccatatgga ctccttgctg gcagcgataa
    13321 tgctcgggtt agttgtcacc ccggcaatgg ggaatatgcg tgccagacgt tcgacttctg
    13381 cgacgttagc ggtgtccaga tacagttcca tgatgtttcc ttttacagtt ccagttcatg
    13441 ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta accagcgcgt tacggaattc
    13501 ttcgtgcatg atgcggcgag cgaggcgcga gaaaatgcgc atgtgctgat cgcccgcagc
    13561 gtgtttgttc agggttaaca tgatgatgaa ttgcgcttca tcatcacccc agcgcaccgg
    13621 cgcttgcaga cgcgcaacgc tgatggtgga ttgctcaatg tgttctgatt tgctgtgtgg
    13681 aatggcaaaa ctaaagccca gaccggtaga gaaaacggcc tcgcgcgccc acaagtcggc
    13741 ttccagttta cgcggatagc ggcagcgccc tgccagcagc aggttatcgg tcatgccttt
    13801 gagcacttct tctttgctgc gccagtcgct ttccagtgtg atgcactcgg cggtgaccag
    13861 cggtgcgtct tgttgggtca tgcggaattg cgccagcagg tgctccactt ccagcgaagt
    13921 acggcaggcc attgcctggt tgagcaactt gcggcactca cggctatcaa gttgcgccat
    13981 ccgcgctttc gccgccggaa ttgatggtgc gctcatgctg agttcatcca gccctaagcc
    14041 gaccagcaac ggcagcacgg aacctttcgc tcccagctca ccgcacagac caatccattt
    14101 accctggcga tgcaccgcct gcacggcgta atcgagcgcc cgcaagaatg ccggattcag
    14161 gctgttgtag tgacgagtaa ccttagcgtt atcgcgatcc actgccagca gatattgcgt
    14221 caggtcatta ctaccaatac taaagaaatc aatctcttcg cagcattgat cgatgatgaa
    14281 catcaccgac ggcacttcca gcataatgcc gagctggatc ttctcatcaa acggaatgtg
    14341 ttcgttacgt agttgctgtt tggcttctgc cagtttttct ttcacccata agatctcttc
    14401 cattgaggag atcatcggga tcatgatttt caggctgccg tgagcggagg cgcgcaggat
    14461 cgagcgtagt tgtgtggtaa ataacgacgc gtattcttca taaatacgca cggcgcgata
    14521 accgaggaac gggtttgcct ctgcgggaat gtttagataa tcaacgggtt tgtcgccgcc
    14581 aatgtccata gtgcgcacaa taatgctgcg tccgttggca gactccagcg cctgacaaaa
    14641 aatgttgtac agctcgcttt cgcccggtgc actggtgcga tccatataga gcatttcagt
    14701 gcggaacaaa ccaacgcctt ccgcaccatt gccgaatgcg gcctgcgctt ccacggagtg
    14761 agcgatgtta gcggcaattt caatgcggat accgtcagcg gtacgggctt gttgggtcag
    14821 ccagacacgc tgttgctcac gcagggcgtc ctgtacgcgg gcttcttgct gataataacg
    14881 agctacggct tctcctggct caaccacaat cgccccggcg ttgccgtcga tataaatcgt
    14941 ttgatgctgc cacggagtga gggcatcaat atccacacca accagcgtcg gaatgttgaa
    15001 cgaacgggca aggatcaccg tatgtgaggt ggtgccgcca cttttcagca acagaccttt
    15061 gaggtgattt ttatccagtt cgaggaactg gctgggggtc agttcatcag ccatacaaat
    15121 ggcggactgc gtcagtttgc ccggtgccgg gaagcgttgc tcaccgtaga tttgctggag
    15181 taactggaag cagacgtcgc gtacgtccag ggcacgttct tgcaggtagc tgctgctgga
    15241 acgggcgaac tcttcgcaaa agtgattcgc actggcgaca atcgcttcgg cgcagcttaa
    15301 tccctcgctg acgcctgcca gtaagtgctg acgtaaagat gtatcgccag ccagggatcg
    15361 gtgagcttcc agtatcgcgc tggtagcacc gtcgctatcc agcagacgaa actcaatgtt
    15421 tttcagtacc agcgtcaggc cgttttccag tgtggattgc tcggcgtcaa cgtctttggc
    15481 tgcgggaaga ttactcagcg cattgagatc taaagaagag atcggcgtca gaatgccgcc
    15541 tgcactaccg ctgcacacgg tgcgggcgcg gataatttgc ggattcagat tggtcagtga
    15601 aaccggcagt ggttccagtt cgtcagattt aacttccgcc agcggcgcat cgcagtgggg
    15661 gaattcatcg cgcagccatt ggcttaaacg ctggtgcgct tcctgttcgt cggccccgga
    15721 aatcagtaac tggcagttat cgcccgccag cgtatcggtg ccaatcagcg caagggcgct
    15781 tttggcgttg cccttgcggt cagtgtgcag gttatgccac tcaatttgtg atgaaaaagt
    15841 attacacagc gtttcaacgt ggcttgccgg acgcgcatgt acgccgttag gtagctcaca
    15901 aataaattcc acaatcaggg ccattgcctc tctcccggta cggttttctg tttacaggat
    15961 aagagcgcac ggcaacggcc tgccatgtga caaatctgcc aaaagctgga caaatgtaat
    16021 gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt tgcggcatgc ttccggttta
    16081 tcgcaagtta tgaggcggat cgcatttttg tactgatatt acaaaagtcc agtaaatggc
    16141 ctttttatcc actgtttgct ccgcctgcga ttgcctattg tttcctccat caaaaatatg
    16201 gacatgggct ttcgcaagcc cgggagcagc ttatgaatga gttggtgcag atcctgaaaa
    16261 atacccgtca gcatttaatg acgggcgttt cacacatgat tcccttcgtg gtatcgggcg
    16321 gtattttgct ggcggtttcc gtcatgttgt atggcaaagg cgcagtgccg gatgccgtag
    16381 ccgatccaaa tctgaaaaaa ctgtttgata tcggcgttgc gggcttgacg ctgatggtgc
    16441 ctttcctcgc cgcttacatt ggttattcca ttgcagaacg ttctgcgctg gctccatgcg
    16501 ctatcggtac ctgggttggt aacagctttg gtgcgggctt ctttggtgca ctgatcgccg
    16561 ggattatcgg cggcatcgtg gtgcattacc tgaagaaaat tccggtgcat aaagttctgc
    16621 gctcggtgat gcctatcttc attattccta tcgtcggcac actgattacc gcaggcatca
    16681 tgatgtgggg cttgggcgag cctgtagggg cgttgaccaa cagcctgact cagtggcttc
    16741 aggggatgca gcagggcagc attgttatgc tggcggtgat catgggtctg atgctggcgt
    16801 tcgatatggg cggtccggtt aacaaagtgg cctatgcctt catgctgatt tgcgttgctc
    16861 agggtgttta taccgtggtg gccatcgctg ccgtgggtat ttgtatcccg ccgctgggga
    16921 tggggctggc gacgctgatt ggtcgtaaaa atttctccgc agaagagcgc gaaaccggca
    16981 aagcggcact ggtgatgggc tgtgttggcg ttactggagg ggcgattcct ttcgccgctg
    17041 ccgatccgct gcgtgtcatt ccttccatca tggtcggttc agtttgtggt gcagtaactg
    17101 cggcgctggt cggtgcgcag tgctatgcag gctggggtgg tctgattgtg ctgccggtgg
    17161 ttgaaggcaa gctgggttat atcgcagcag tggctgtcgg agcagtggtg acggctgttt
    17221 gtgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc ttcgactgat gaaaaagaag
    17281 acgacctgga tttggatttt gaaattaatt aattgaggaa cgagccatga cgaaaattat
    17341 tgcagtaacc gcatgtcctt caggtgttgc ccatacctat atggcagcag aggcgctgga
    17401 aagtgccgcg aaagcgaaag gctgggaagt gaaagtagaa acctaggggt caattggtct
    17461 ggaaaatgaa ctgactgcgg aagatgtggc gagcgctgat atggtgattc tgaccaaaga
    17521 tatcggcatc aagtttgaag agcgttttgc tggcaagacc atcgtgcgcg tcaacatcag
    17581 cgatgcagta aaacgtgctg acgccattat gagcaaaatt gaagctcacc tggcgcaaac
    17641 cgcttaagtt cttctcccgc tcgcaagggc gggttcgctt tcccacagga gttcctcatg
    17701 acgaatcgta tctctcgcct caaaactgca ctgtttgcca atacccgtga aatctcgctg
    17761 gagcgggcgc tgctttatac cgccagccat cggcaaaccg aaggcgaacc ggtgatattg
    17821 cgccgggcga aagcaacagc gtatatcctt gaacatgttg aaatttcgat tcgtgatgaa
    17881 gaactgattg ccggtaaccg caccgtaaaa ccgcgcgccg ggattatgtc gccggaaatg
    17941 gacccttact ggctgctgaa agagctggat caattcccga cgcgtccgca ggaccgcttt
    18001 gctatcagcg aagaagataa acgtatctac cgtgaagagt tgttcccgta ctgggaaaaa
    18061 cgttcgatga aagatttcat caacgggcag atgacagatg aagtaaaagc cgcgaccaac
    18121 acgcagattt tcagcatcaa ccagacggat aaaggccagg ggcacattat tattgattac
    18181 ccacgcctgt tgaatcacgg tctgggtgag ctggtggcac agatgcagca acattgtcag
    18241 caacagccgg agaatcactt ttatcaggcc gcgttgttac tgctggaagc ctcgcagaaa
    18301 cacattttgc gttacgccga actggcggaa acgatggcgg caaactgcac agatgcacag
    18361 cgtcgcgaag aactgctgac tattgcggag atctctcgcc ataacgccga acataagccg
    18421 cagacgttct ggcaggcgtg ccagttattc tggtacatga acatcattct gcaatacgaa
    18481 tccaacgcca gttcgctgtc tttagggcgc ttcgaccagt atatgttgcc gttctatcag
    18541 gcatctttaa cccagggcga agatccggcg ttcctgaaag aactgctcga atctttatgg
    18601 gtgaaatgca acgacatcgt gctgttgcgc tccaccagca gcgcgcgtta tttcgcaggt
    18661 ttcccgaccg gctataccgc actgctcggc gggttaaccg agaacggacg tagcgcggtg
    18721 aacgtgcttt cgttcctttg ccttgacgcc tatcaaagcg tgcaattacc gcaaccgaac
    18781 ctcggcgtgc gcactaacgc cttgatcgac acgccgttcc tgatgaaaac cgccgaaacc
    18841 attcgcctcg gcaccggtat tccgcaaatc tttaacgatg aagtggtggt accagcgttc
    18901 ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt ccgtagtggg ctgtgtggaa
    18961 ttatctattc ccggcagaac ctacggcttg catgacatcg cgatgtttaa tctgctgaaa
    19021 gtgatggaaa tctgcctgca tgaaaatgaa ggcaatgctg cgctgactta tgaaggttta
    19081 ctggagcaga tccgcgccaa gatcagccac tacatcaccc tgatggttga gggcagcaat
    19141 atttgtgata tcggccatcg cgactgggca cctgtaccgc tgctctcgtc ttttatcagc
    19201 gattgtctgg aaaaaggtcg cgatattacc gatggcggcg cgcgttataa cttctccggc
    19261 gtacagggga tcggtatcgc caacctgagc gattctctcc atgcgttgaa agggatggtt
    19321 tttgagcaac agcgtttaag ttttgacgaa ttgctgtcgg tattaaaagc caactttgca
    19381 acgccagaag gcgaaaaagt ccgtgctcgc ttaattaacc gcttcgagaa atacggtaac
    19441 gatatcgacg aggtggataa catcagcgcc gaactgttac gccactactg caaagaagtg
    19501 gaaaaatacc agaacccgcg cgcggttact tcacgccggg atcgtatacc gtttctgctc
    19561 acgtcccgtt gggatcggtg gttggcgcga cgccagacgg tcgttttgcc ggggaacaac
    19621 tggcagacgg cggcttgtca cctatgctgg gccaggacgc acaagggcca acagcggtac
    19681 tgaagtcagt cagtaagctc gataatacgc tgctgtctaa cggtacgttg ctgaacgtga
    19741 aattcactcc ggcgaccctg gaaggtgaag cgggattacg caaactggcc gacttcttac
    19801 gggcgtttac ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac gccgacacgt
    19861 tgcgggaagc gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat
    19921 acagcgcctt ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag
    19981 cgcatcagct gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt
    20041 cagcgttatt cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt
    20101 ccgcatcttt gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat ccagacggta
    20161 cgcagagagg cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc
    20221 tccggggcgt ttgaacggat tggtcgcgat atcagccttg acgctctgga acgggaagtg
    20281 atgaaagatg acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa
    20341 gtgttaatgc aggcggagtt tgctacccgt tttttacagc gactgcggct gtggggtgtg
    20401 tcatgcgcca ttgaaactgc cggagacgcg ccagcaagca agctgttacc gctggcgaaa
    20461 ttgtgcgatg aagtgctgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg
    20521 gtgaagatga acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc
    20581 aacgtgatcc cgcgtttacc gttgatccct ggtttcacgc tcagcaggga gaatatgcag
    20641 caggcgctgg atgtactgat cccgctgaaa atcaggcaga tccatctgtt accgtttcat
    20701 cagtacggcg aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct
    20761 gcgccgtcgt cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc cggatttcag
    20821 gttaccgtgg gaggttaaaa tggcatacct ggtggcagta accgcctgcg taagcggcgt
    20881 ggcgcatact tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg
    20941 cgtcagcatt gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat
    21001 ccgtcgggcg gatgttgctc tgttgattac ggatatcgag cttgccggtg ccgagcgatt
    21061 tgaacattgc cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt
    21121 aatgagcgcg gtgcgcaaag tgctttctgc gccgcagcaa acccatctta ttctggagta
    21181 gtcggttttt ctgtcagttg gctgtggtac tgccggcgat attccgacgg cgagcgttcg
    21241 gtgtttttac gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc
    21301 acttctttta ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg
    21361 tgattcaggt attcgttaaa accaatggcc cccgtttttt gaaacaggtg cgagagatag
    21421 tttggtgaaa tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt
    21481 tcgtcgatat aatcgcgaat agcttcaaat agtgcctggc tgcgtgaggc ggtctggatt
    21541 tggctgccga gcaaatcgcg gcagtggcta agcaggctgg ctacgataag ccttgccgtt
    21601 tgctgctcct gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctata
    21661 cgtgggccgc gtcgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat
    21721 tgcacgacgc tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt ggtagcgggt
    21781 gttttccatt gtgggaaatt ccagccacca gcggcaacgt acagcacatc gccgggaact
    21841 aacgttgctc cagcgccggt atcaacaaac tcaccttcca gcacaatttc cagccgtgga
    21901 aaatcgacct gatacgcaag atcgggcacc gggccgttac tgctggcaaa gtaaatctgg
    21961 cgcagggaca gcgggccgtt gatcaggcgg gagagcagat agctgacgcc gtgatacatg
    22021 ttattccctt aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct
    22081 gaaaagtgtg gcctgagggg agttcgatgt aaacattgcg gcagttgtat tgccggatgt
    22141 ggcgtaaacg ccttatccag cctaccgccg cgatctgtag gccggataag acgcgtcaag
    22201 cgtcgcatcc ggcaaatagc gcctggctga taattactga ttacctacct ggtcgccata
    22261 cggcagagtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa tccagcgggt
    22321 agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt cgtaagataa
    22381 gcccgccaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac gatcgacatc
    22441 ctgtgagaaa tcacggggat gagtcgcttg ccatttttcc gacgtccaca gcaggaacgg
    22501 aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt tgtgcggcgg
    22561 cgtgtcgtaa acctcttcac cgtggtcaga gaagtaaacc aggaatccgt tcgggtctgc
    22621 cgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt tagcgttgtc
    22681 ataatcgtta tatgattcca gctcttctgc gcttaatcct ggcggaacat gatcggtatt
    22741 gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat gcgtacccag
    22801 cagatgaacg atgatcagtt tcttcggcgc agggtcattc agcacatcct ggaacggctt
    22861 cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca tgtagtactg
    22921 cttgtcggtc tggcgcgaaa ataccgtcag catggtattg cgggcggtca tcgtctgctg
    22981 gttggtgatc cagaaggttt tataacccgc ctgtttcatc atgttcatca gcgacggctg
    23041 cgtcagatac agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt gcaggatttc
    23101 aatggtgtac ggacgagacg taactacgtt attaaacacg gtcagattcg gatcggtttt
    23161 atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac tcatgcgtcc
    23221 gcgctgggtc gactcgccaa tcaccagcac taaagtgcgc ggttcgttac ccgattcatc
    23281 tttgaaatta gccagtggcg gcaaggcatt attttcattc agtaactttg ttagcgagtt
    23341 tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg caggctccat
    23401 acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa acgtattcat
    23461 ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat aacgccacgg
    23521 ctttggaata tagaccgggc gcaggcgtgt ccacagcaga actgccaccg ccgtataggc
    23581 cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc tggcttcgtt
    23641 ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc cgtagatgac
    23701 gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta ctgcggcaat
    23761 aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc tgaataacag
    23821 cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg cctgtagcag
    23881 agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc taaaaagggg
    23941 tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa atatcgccga
    24001 atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa taaaatgcag
    24061 acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa ccctcgcgca
    24121 aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc tgccgcaatc
    24181 ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg gccttctttt
    24241 tctgcctggc gggagcggtg cagcaactcg gcctgcaata cgttcagcgg gtcggtgtaa
    24301 atattccgta gctgaataga ctctgcaatc cacggcagat cggccatcag atgggaatcg
    24361 ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa ctctttacct
    24421 aacggccaca gtgctttgtc taccagacgt tgatcatagt attccgccaa ccacaggtct
    24481 gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg ccaatcgcgg
    24541 cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag cgccgtacct
    24601 gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat ccacggtatg
    24661 gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga acccaacggc
    24721 agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa atctttgttt
    24781 tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc cataatgcga
    24841 cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat cgccccggtg
    24901 taaagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg gatcatctcg
    24961 ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga cagcagcgcc
    25021 gcatgggcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa caacgtcagc
    25081 tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata ttgcgcccag
    25141 gaagctgcca tcacgcccgc atcttttgct gagtcggaat agccaatcat caccatctgt
    25201 ttgccctgaa tcaggccgcg ataccagtcg atattgagca gctgggtcat gacatcgttg
    25261 gcgttgttca ggtcatcgag ggtttcaaac agcggagcaa ccggcatcgc aaacccgata
    25321 cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt cgccatcgag
    25381 atcacgtagg cggcaatgga gccttgcggc gcttcggcaa tcacctggca ggtatcgagc
    25441 acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg tttggagttc
    25501 agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta gtcgccgata
    25561 ccgaggtagc gggtcagctc gcccagcgct tcggtatggc gggtgctctc ctgacggata
    25621 tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc gagcagatcg
    25681 ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc gtagagcggt
    25741 tcccacagtt cttcgttttg tgtcagcagc ccttctggtt ttggcagttc ttcgcctttc
    25801 aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt tttcatcaga
    25861 tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc aggggtcgct
    25921 tcaaccatcg acagttcaga aaccagaacc tgaatatctt tcaggaacag atcggtggct
    25981 ttccagcgac tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt cgggttgccg
    26041 tcgcggtcac cgcccatcca cgaagtaaaa cggaccggaa caaattcgac gggcagtttg
    26101 tagccgaggt tctcttccag ttgttcgttc agttcgcgca agtaattcgg tacgccttgc
    26161 cacaggctgt tttctactac ggcaaagccc catttggctt catctaccgg gcttggacgc
    26221 agcttacgga tttcatcagt atgccatgac tgggcgatta actggcgcag gcgacgcatc
    26281 agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa gcaggcgttc
    26341 acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg agccgtgagg
    26401 accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct cagttccggc
    26461 tggtttttca gtttgcgcag ggtgcgggcg atcacttccg ggttgctggc agcttcgcct
    26521 ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa ctgactaaac
    26581 gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt gagcaactcc
    26641 tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat agtttctacg
    26701 cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac tttgccgagc
    26761 atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca gacaccccat
    26821 cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg ctcgggcttt
    26881 gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc tgcgaaatcg
    26941 cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg ctttaaataa
    27001 gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg taggtcggat
    27061 aagatgcgca agcatcgcat ccgacattat tgcggcaccg gagtttggca acggtgccgg
    27121 atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg gtgaattacc
    27181 tggataatca actcacgggt gggtttaata aaccgcgtct ccagatattc atcaggctga
    27241 tgagcttgat tgatggaacc cggaccgagc accagcgtcg ggcataacgt ttgaataaac
    27301 ggcgcttcgg tacagtaatt caccacttcg gtttttgctc cgagcaattt ctcaaccact
    27361 tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg cagctcgtcg
    27421 accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt gagcaaacca
    27481 ttaagttcat tgagtgtcat gctaggcagc ggacgaatat ccatatgcaa ctcacagcaa
    27541 gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag cgtagggtat
    27601 ggcacggtaa atgcttcgta gtgataacgt tctttcaggt tatcgcgcaa ttgcaaaata
    27661 tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg atcgctggag
    27721 tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg tgcgcgtacc
    27781 ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc ggtagtttcg
    27841 gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag aatgtagagc
    27901 ggttttttca gtttcgtgac gtcgacatcg cgtagcgtat caaggataaa cgcaaaaaag
    27961 cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt cagtgtaaac
    28021 ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc cgccagcaac
    28081 aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg agttcctggc
    28141 acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag cagagtgatt
    28201 aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg tgtggcaatc
    28261 agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga cacacctctg
    28321 gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta acgttgagcg
    28381 taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa ccagacataa
    28441 gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc tacgctggcg
    28501 cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct ttgactgttt
    28561 cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag ctaaaaggca
    28621 tcgttgagct gccgttgcag ccgatgtcgg atatcagcga gtttagccca ggggtggacg
    28681 tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag tttcttgaag
    28741 cgggctgcgt ggtgttcgac ctttccggtg cgtttcgtgt taacgacgcc accttctatg
    28801 aaaaatatta cggctttacc catcaatacc cggaactgct ggaacaggca gcctacggtc
    28861 tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg ccaggctgtt
    28921 atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt cttgacctca
    28981 atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt aaggcagcga
    29041 tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttact catcgccatc
    29101 aaccagagat cgccacacac ctcggtgctg acgttatctt caccccacat ctgggcaatt
    29161 tcccgcgcgg cattctcgaa accattacct gccgcctgaa atcgggtgtg acccaggcgc
    29221 aagtcgcgca agtgttacag caggcgtatg cccataaacc gctggtgcgg ctgtatgaca
    29281 aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc gggtttgccg
    29341 ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa ggcgcggcgg
    29401 cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag tctcttattt
    29461 aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct ggatagtgaa
    29521 gaggcgctgg aacgtctgtt tagcacactg gtgaattatc gtgagtcaca tcagcgtccg
    29581 ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg gctgaatctg
    29641 ccggtgaaaa agaaaaacgg cctgcgggtg acgcctgctg atcagataga cattatcacc
    29701 ggagcactgg cgggaacggc aaataaaacc ctgttggcat gggcgaagaa acatcagatt
    29761 gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca gcttgatgaa
    29821 gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa ctccttgctg
    29881 gagaacggtt atctgccggt ggtcagctcc attggcgtaa cagacgaagg gcaactgatg
    29941 aacgtcaatg ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc ggatctgatt
    30001 ttgctctccg acgtcagcgg cattctcgac ggcaaagggc aacgcattgc cgaaatgacc
    30061 gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat gatagtgaaa
    30121 gtgaacgcgg cgctggatgc ggcccgcacg ctgggccgtc cggtagatat cgcctcctgg
    30181 cgtcatgcgg agcagcttcc ggcactgttt aacggtatgc cgatgggtac gcggatttta
    30241 gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa ataaggaaac
    30301 agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg gttcaaacaa
    30361 ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt tggctctgtg
    30421 gcctggtcca aagccctggt cacggtaggc gtgttaaccg cagaagagca ggcgcaactg
    30481 gaagaggcgc tgagcgtgct gctggaagat gttcgcgcca ggccacaaca aatccttgaa
    30541 agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa agtgggccag
    30601 ttaggcaaaa agctgcatac cgggcgtagt cgtaacgatc aggtagcgac tgacctgaaa
    30661 ctgtggtgca aagataccgt tagcgagtta ctgacggcta accgacagct gcaatcggcg
    30721 ctggtggaaa ccgcacaaaa caatcaggac gcggtcatgc caggttacac tcacctgcaa
    30781 cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat gctggcgcgt
    30841 gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt aggctgtggc
    30901 gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg gctgggcttt
    30961 gcttcggcga cccgtaacag tctggacagc gtttctgacc gtgaccatgt gttggaactg
    31021 ctgtcggctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga tctgattttc
    31081 tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc cggttcatca
    31141 ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa atgcggtcgg
    31201 gtgcagggtg cgttaaccgg catgatgatg acgctgaaag gtttgccgct ggcttacaac
    31261 aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg gctggactgc
    31321 ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg ttgccaggaa
    31381 gcggctcagc agggttacgc caacgccacc gaactggcgg attacctggt ggcgaaaggc
    31441 gtacctttcc gcgaggcgca ccatatcgtg ggtgaagcgg tggtggaagc cattcgtcag
    31501 ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc ggtgattggc
    31561 gaagatgtct atccgatttt gtcgctgcaa tcgtgcctcg acaagcgtgc ggcaaaaggc
    31621 ggcgtctcac cgcagcaggt ggcccaagcg attgcttttg cgcaggcgcg gttggggtaa
    31681 gaacatttat atgtacaaat ttgagcctgg cttatcgccg ggctttttta tggcaaaaaa
    31741 aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga gttgagaaac
    31801 tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt ctgtgagcaa
    31861 ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct atcgccatga
    31921 actatcgtgg cgatggagga tggataatga atattcgtga tcttgagtac ctggtggcat
    31981 tggctgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc cagccgacgc
    32041 ttagcgggca aattcgtaag ctggaagatg agctgggcgt gatgttgctg gagcggacca
    32101 gccgtaaagt gttgttcacc caggcgggaa tgctgctggt ggatcaggcg cgtaccgtgc
    32161 tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg atgtccggac
    32221 cgctgcatat tggtttgatt cccacggttg gaccgtacct gctaccgcat attatcccga
    32281 tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag acccaccagt
    32341 tactggcgca actggacagt ggcaaactcg attgcgtgat cctcgcgctg gtgaaagaga
    32401 gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct atctatgaag
    32461 atcacccgtg ggcgaaccgc gaatgcgtac cgatagccga tctggcaggg gaaaaactgc
    32521 tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt tttgaagccg
    32581 gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc aacatggtgg
    32641 cggcaggtag cgggatcact ttactgccag cgctggctgt gccgccggag cgcaaacgcg
    32701 atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt ggcctggttt
    32761 atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc atccgagcaa
    32821 gaatggatgg ctatttcgat aaagtgttaa aacaggcggt ttaaaccgtt taacgcagct
    32881 acccgatagg cttccgccat ggtcgggtag ttaaaggtgg tgttgacgaa gtactcaata
    32941 gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat ttcggcagcg
    33001 cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa caaaattttc
    33061 agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa ctgggcgcgg
    33121 cccacttcat atggcacttt catcgcggtc agctgctgtt cggttttgcc cacagagctg
    33181 atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgcgc ggtggcttcg
    33241 ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga tgccaggctc
    33301 ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg atacatgctg
    33361 ttgaccttca gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa cgccagcgaa
    33421 tcggtattac cggtacgacc gttggcatag agcaggcagt cagctttcag ttttttaccc
    33481 gacttcagat gcatgatcac accgtcgtca cagccttcga tcttctcgta ctcttcgttg
    33541 tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga catctcttga
    33601 tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac atccataccg
    33661 cggaagatcg acgcatattc acagccgatc actccagcac catagataag tacatggcgc
    33721 ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg ggtgaaatca
    33781 acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc agcggttagt
    33841 gtttcgacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac aaagcgagcg
    33901 ttcccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat gcgcgtttgt
    33961 tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg gagcagtcgc
    34021 gaatggtcgc tgtatagtgg gttttgattg aattctataa tgcggctgac ggcgtgacgg
    34081 agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt ttgataacgc
    34141 tcgataactg cgacgcgcac accttgctta accaggccca ttgcagcgcc ttcgccgccg
    34201 gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt agggcttacc
    34261 tgttcttata cataaaagca acagaatggt aacattctat cgcgggtaag ccaattgatc
    34321 cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc ttacttttgc
    34381 cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca ggtttccgct
    34441 gtacgtaaaa gaaccggcca gtaaatatgt tttattgcgt taccgttcat tcacaatact
    34501 ggagcaatcc agtatgttca ttctctggta tagtgccagc agtacttttg gcaaggattc
    34561 agacatcgtg atgggcataa gagcgcaaca aaaagaaaaa acccgccgtt cgctggtgga
    34621 agccgcattt agccaattaa gtgctgaacg cagcttcgcc agcctgagtt tgcgtgaagt
    34681 ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg catttccgcg acgtagacga
    34741 actgggtctg accatggttg atgagagcgg tttaatgcta cgccaactca tgcgccaggc
    34801 gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc tcggtctcca catttatgga
    34861 gttcatcggt aataatccta acgccttccg gttattattg cgggaacgct ccggcacctc
    34921 cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg aacttgcgga
    34981 ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag ccgaagcaat
    35041 ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac gtcggcgtcg aacaacgtcg
    35101 gcaattagaa gagcgactgg tactgcaact gcgaatgatt tcgaaagggg cttattactg
    35161 gtatcgccgt gaacaagaga aaaccgcaat tattccggga aatgtgaagg acgagtaatg
    35221 aaacaagcaa atcaagatag aggtacgctg ctgctggcgt tagttgctgg cttatcgatt
    35281 aatggtactt tcgcagcgct gtttagctcc attgtgccat tttctgtatt cccgattatt
    35341 tccctggtgc tgacggttta ctgcctgcat caacgttatc ttaatcgcac tatgccggta
    35401 ggcttgccgg gtctggcagc tgcctgtttt attctcggcg tactgctgta cagcacggta
    35461 gttcgtgcgg aatatccgga tatcggctct aacttcttcc cggcagtact ctccgtcatt
    35521 atggtgttct ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc tgagtaatcg
    35581 gtattatgcc ggatgctggc gcatccggca tgggttttac ttcgcggtca gtaatacgcc
    35641 gcactccata tggtgcgtgt aggggaactg atcaaacaga gccagacgtt cgaccttgtg
    35701 cgtctggctt aatgtttcca gattcttgca taacgtttcc gggttacagg agatgtacaa
    35761 aatacacgga tacgcctgca ccattttctc ggtttcactg tccagaccgc tgcgcggtgg
    35821 gtcgacaaaa atggtttcgc actgataact ctttaagtcg atcccttgca ggcggttaaa
    35881 ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa taatttgtac
    35941 gttatcaata tggttagctg cgatgttgta ttgcgcagca gcaaccgacg gcttagcgat
    36001 ttcggtggct aatacccgat caaaattgcg cgccagcgct aatgaaaagt taccgttgcc
    36061 gcagtacagc tccagtaaat cgccttttga gcctttggtt acgtccagcg cccattccag
    36121 catctgaata ttcatcgccg cgttcggctg ggtaaagctg ttttctacct gacggtagat
    36181 catctctttc cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca gctcgatttt
    36241 ggttttcgtt gcccgaccaa tcagatgcac attcagattc tgcgcgcgca gtgcatcgcg
    36301 cagggcctcc gcttcctgac gccactcatc atccagcttc ttatggtata gcagggaaac
    36361 caccgcctga ttactcagcg tggtgaggta atcaatctgg aacaacttgt ggcgcagaac
    36421 gggattatta cgcacacccg caatcatcgc cgtcatcaac tggttgataa gttcactggc
    36481 ggcggggaag ctatccacgc ggatacggct tttggtttgt tgatcgaaaa tgatgtgata
    36541 caggtcatcg ccatcgtgcc agatgcggaa ctccgcacgc atccggtaat gactgaccgg
    36601 cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc atcatacttt gcaaacgtac
    36661 cactttttcg gctaactgcg cttcatactg ttctgttgga aggtgttcgg gggtcatgat
    36721 gtatcctgaa aaattaaagt acgcggcgat tgtagggatt gctcatcaga tgtccagatc
    36781 ttgatgaatt cctatttgtg agctacgtct ggacagtaac ttgttacaac ctgtagcatc
    36841 cacttgtcgg tcctgtgagt taatagggaa tccagtgcga atctggagct gacgcgcagc
    36901 ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc catccggtgg gaagtcatca
    36961 tctcttagta tcttagatac ccctccaagc ccgaagacct gccggccaac gtcgcatctg
    37021 gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat tgtggatgct
    37081 ttacaatgat taaaaaagct tcgctgctga cggcgtgttc tgtcacagcc ttttccgctt
    37141 gggcacagga taccagcccg gatactctcg tcgttactgc taaccgtttt gaacagccgc
    37201 gcagcactgt gcttgcacca accaccgttg tgacccgtca ggatatcgac cgctggcagt
    37261 cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc caaaacggcg
    37321 gttcaggtca gctctcatct atttttattc gcggtacaaa tgccagtcat gtgttggtgt
    37381 taattgatgg cgtacgcctg aatctggcgg gggtgagtgg ttctgccgac cttagccagt
    37441 tccctattgc gcttgtccag cgtgttgaat atatccgtgg gccgcgctcc gctgtttatg
    37501 gttccgatgc aataggcggg gtggtgaata tcatcacgac gcgcgatgaa cccggaacgg
    37561 aaatttcagc agggtgggga agcaatagtt atcagaacta tgatgtctct acgcagcaac
    37621 aactggggga taagacacgg ggaacgctgt tgggcgatta tgcccatact catggttatg
    37681 atgttgttgc ctatggtaat acaggaacgc aagcgcagac agataacgat ggttttttaa
    37741 gtaaaacgct ttatggcgcg ctggagcata actttactga tgcctggagc ggctttgtgc
    37801 gcggctatgg ctatgataac cgtaccaatt atgacgcgta ttattctccc ggttcaccgt
    37861 tgctcgatac ccgtaaactc tatagccaaa gttgggacgc cgggctgcgc tataacggcg
    37921 aactgattaa atcacaactc attaccagct atagccatag caaagattac aactacgatc
    37981 cccattatgg tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa tacaccgtcc
    38041 agtgggcaaa caatgtcatc gttggtcacg gtagtattgg tgcgggtgtc gactggcaga
    38101 aacagactac gacgccgggt acaggttatg ttgaggatgg atatgatcaa cgtaataccg
    38161 gcatctatct gaccgggctg caacaagtcg gcgattttac ctttgaaggc gcagcacgca
    38221 gtgacgataa ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc ggttgggaat
    38281 tcatcgaagg ttatcgcttc attgcttcct acgggacatc ttataaggca ccaaatctgg
    38341 ggcaactgta tggcttctac ggaaatccga atctggaccc ggagaaaagc aaacagtggg
    38401 aaggcgcgtt tgaaggctta accgctgggg tgaactggcg tatttccgga tatcgtaacg
    38461 atgtcagtga cttgatcgat tatgatgatc acaccctgaa atattacaac gaagggaaag
    38521 cgcggattaa gggcgtcgag gcgaccgcca attttgatac cggaccactg acgcatactg
    38581 tgagttatga ttatgtcgat gcgcgcaatg cgattaccga cacgccgttg ttacgccgtg
    38641 ctaaacagca ggtgaaatac cagctcgact ggcagttgta tgacttcgac tggggtatta
    38701 cttatcagta tttaggcact cgctatgata aggattactc atcttatcct tatcaaaccg
    38761 ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc acctctcacc
    38821 tgacagttcg tggtaaaata gccaacctgt tcgacaaaga ttatgagaca gtctatggct
    38881 accaaactgc aggacgggaa tacaccttgt ctggcagcta caccttctga accacgtccc
    38941 accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg tctatgacga gatccggcat
    39001 ctcttaccgg atctccatta catttatgct ttcgataacg tcgctttccc gtatggcgaa
    39061 aaaagcgaag cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt gcaagaacgt
    39121 tatccccttg cgctggctgt ggtcgcttgc aacactgcca gtaccgtttc acttcctgca
    39181 ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa acctgctgca
    39241 cgtctgacgg caaatggcat tgtcggatta ctggcaaccc gcggaacagt taaacgttct
    39301 tatactcatg agctgatcgc gcgtttcgct aatgaatgcc agatagaaat gctgggctcg
    39361 gcagagatgg ttgagttggc tgaagcgaag ctacatggcg aagatgtttc tctggatgca
    39421 ctaaaacgta tcctacgccc gtggttaaga atgaaagagc cgccagatac cgttgtattg
    39481 ggttgcaccc atttccctct actacaagaa gaactgttac aagtgctgcc agagggaacc
    39541 cggctggtgg attctggcgc agcgattgct cgccgaacgg cctggttgtt agaacatgaa
    39601 gccccggatg caaaatctgc cgatgcgaat attgcctttt gtatggcaat gacgccagaa
    39661 gctgaacaat tattgcccgt tttacagcgt tacggcttcg aaacgctcga aaaactggca
    39721 gttttaggct gatttggttg aatgttgcgc ggtcagaaaa ttattttaaa tttcctcttg
    39781 tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg gcaaacacgc
    39841 cgccgggtca gcggggttct cctgagaatc tcaacagaga aaagcaaaaa aatgc
//
LOCUS       DAZSBF010000008        38337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZSBF010000008 DAZSBF010000000
VERSION     DAZSBF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.008"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHXWD_000292"
     CDS             59..1423
                     /locus_tag="ACHXWD_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7619321.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHXWD_000293"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHXWD_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR7619322.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHXWD_000294"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHXWD_000294"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR7619323.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHXWD_000295"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHXWD_000295"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR7619324.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHXWD_000296"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHXWD_000296"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR7619325.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHXWD_000297"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHXWD_000297"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR7619326.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHXWD_000298"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHXWD_000298"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR7619327.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHXWD_000299"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHXWD_000299"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR7619328.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHXWD_000300"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHXWD_000300"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR7619329.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHXWD_000301"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHXWD_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR7619330.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHXWD_000302"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHXWD_000302"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR7619331.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHXWD_000303"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHXWD_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR7619332.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHXWD_000304"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHXWD_000304"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR7619333.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHXWD_000305"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHXWD_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR7619334.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHXWD_000306"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHXWD_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR7619335.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHXWD_000307"
     CDS             13749..14288
                     /locus_tag="ACHXWD_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR7619336.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHXWD_000308"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHXWD_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR7619337.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHXWD_000309"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHXWD_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR7619338.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHXWD_000310"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHXWD_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR7619339.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHXWD_000311"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHXWD_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR7619340.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHXWD_000312"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHXWD_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR7619341.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHXWD_000313"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHXWD_000313"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR7619342.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHXWD_000314"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHXWD_000314"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR7619343.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHXWD_000315"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHXWD_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR7619344.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHXWD_000316"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHXWD_000316"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR7619345.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHXWD_000317"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHXWD_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR7619346.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHXWD_000318"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHXWD_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR7619347.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHXWD_000319"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHXWD_000319"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR7619348.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHXWD_000320"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHXWD_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR7619349.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHXWD_000321"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHXWD_000321"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR7619350.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHXWD_000322"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHXWD_000322"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR7619351.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHXWD_000323"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHXWD_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR7619352.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHXWD_000324"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHXWD_000324"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR7619353.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHXWD_000325"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHXWD_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR7619354.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHXWD_000326"
     CDS             complement(37465..37920)
                     /locus_tag="ACHXWD_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619355.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHXWD_000327"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHXWD_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9471 a        10314 c         9728 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgcctggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZSBF010000009        37768 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZSBF010000009 DAZSBF010000000
VERSION     DAZSBF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.009"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHXWD_000328"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHXWD_000328"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR7619356.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHXWD_000329"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHXWD_000329"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR7619357.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHXWD_000330"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHXWD_000330"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR7619358.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHXWD_000331"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHXWD_000331"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR7619359.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHXWD_000332"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHXWD_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR7619360.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHXWD_000333"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHXWD_000333"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR7619361.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHXWD_000334"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHXWD_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR7619362.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHXWD_000335"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHXWD_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR7619363.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHXWD_000336"
     CDS             6537..6773
                     /locus_tag="ACHXWD_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR7619364.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHXWD_000337"
     CDS             6776..7633
                     /locus_tag="ACHXWD_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR7619365.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHXWD_000338"
     CDS             7654..9645
                     /locus_tag="ACHXWD_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR7619366.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHXWD_000339"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHXWD_000339"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR7619367.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHXWD_000340"
     CDS             complement(10248..11144)
                     /locus_tag="ACHXWD_000340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR7619368.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHXWD_000341"
     CDS             complement(11193..11432)
                     /locus_tag="ACHXWD_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR7619369.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHXWD_000342"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHXWD_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR7619370.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHXWD_000343"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHXWD_000343"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR7619371.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHXWD_000344"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHXWD_000344"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR7619372.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHXWD_000345"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHXWD_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR7619373.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHXWD_000346"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHXWD_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHXWD_000347"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHXWD_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR7619374.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHXWD_000348"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHXWD_000348"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR7619375.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHXWD_000349"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHXWD_000349"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR7619376.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHXWD_000350"
     CDS             complement(21570..22739)
                     /locus_tag="ACHXWD_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7619377.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHXWD_000351"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHXWD_000351"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR7619378.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHXWD_000352"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHXWD_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR7619379.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHXWD_000353"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHXWD_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR7619380.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHXWD_000354"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHXWD_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR7619381.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHXWD_000355"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHXWD_000355"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR7619382.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHXWD_000356"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHXWD_000356"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR7619383.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHXWD_000357"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHXWD_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR7619384.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHXWD_000358"
     CDS             complement(30320..31576)
                     /locus_tag="ACHXWD_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR7619385.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHXWD_000359"
     tRNA            31884..31960
                     /locus_tag="ACHXWD_000359"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHXWD_000360"
     tRNA            31965..32041
                     /locus_tag="ACHXWD_000360"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHXWD_000361"
     CDS             32149..32454
                     /locus_tag="ACHXWD_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR7619386.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHXWD_000362"
     CDS             32580..34184
                     /locus_tag="ACHXWD_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR7619387.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHXWD_000363"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHXWD_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR7619388.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHXWD_000364"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHXWD_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR7619389.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     VVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHXWD_000365"
     CDS             complement(35794..36462)
                     /locus_tag="ACHXWD_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR7619390.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHXWD_000366"
     CDS             complement(36526..37164)
                     /locus_tag="ACHXWD_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR7619391.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..>37768)
                     /locus_tag="ACHXWD_000367"
     CDS             complement(37177..>37768)
                     /locus_tag="ACHXWD_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HFR7619392.1"
                     /translation="GLPLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLR
                     VCLHNAVTLCNWVWPMTVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIF
                     TLHRAYTVKLMQTKDMRNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYK
                     EMGWDPQLGCPTRETLQRLGLEDIAADLAAHNLLPA"
BASE COUNT         9023 a         9930 c         9380 g         9435 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaacca cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccg
//
LOCUS       DAZSBF010000010        36768 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZSBF010000010 DAZSBF010000000
VERSION     DAZSBF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36768
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.010"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(117..926)
                     /gene="pduF"
                     /locus_tag="ACHXWD_000368"
     CDS             complement(117..926)
                     /gene="pduF"
                     /locus_tag="ACHXWD_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR7619393.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
     gene            1438..1722
                     /gene="pduA"
                     /locus_tag="ACHXWD_000369"
     CDS             1438..1722
                     /gene="pduA"
                     /locus_tag="ACHXWD_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR7619394.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            1719..2531
                     /gene="pduB"
                     /locus_tag="ACHXWD_000370"
     CDS             1719..2531
                     /gene="pduB"
                     /locus_tag="ACHXWD_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HFR7619395.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            2550..4214
                     /gene="pduC"
                     /locus_tag="ACHXWD_000371"
     CDS             2550..4214
                     /gene="pduC"
                     /locus_tag="ACHXWD_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR7619396.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            4224..4892
                     /locus_tag="ACHXWD_000372"
     CDS             4224..4892
                     /locus_tag="ACHXWD_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR7619397.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            4907..5425
                     /gene="pduE"
                     /locus_tag="ACHXWD_000373"
     CDS             4907..5425
                     /gene="pduE"
                     /locus_tag="ACHXWD_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR7619398.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            5435..7267
                     /locus_tag="ACHXWD_000374"
     CDS             5435..7267
                     /locus_tag="ACHXWD_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR7619399.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            7257..7607
                     /locus_tag="ACHXWD_000375"
     CDS             7257..7607
                     /locus_tag="ACHXWD_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR7619400.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            7627..7902
                     /gene="pduJ"
                     /locus_tag="ACHXWD_000376"
     CDS             7627..7902
                     /gene="pduJ"
                     /locus_tag="ACHXWD_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR7619401.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            7926..8348
                     /locus_tag="ACHXWD_000377"
     CDS             7926..8348
                     /locus_tag="ACHXWD_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR7619402.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            8351..8983
                     /locus_tag="ACHXWD_000378"
     CDS             8351..8983
                     /locus_tag="ACHXWD_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR7619403.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            8980..9471
                     /gene="pduM"
                     /locus_tag="ACHXWD_000379"
     CDS             8980..9471
                     /gene="pduM"
                     /locus_tag="ACHXWD_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR7619404.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            9475..9750
                     /gene="pduN"
                     /locus_tag="ACHXWD_000380"
     CDS             9475..9750
                     /gene="pduN"
                     /locus_tag="ACHXWD_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR7619405.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            9762..10769
                     /gene="pduO"
                     /locus_tag="ACHXWD_000381"
     CDS             9762..10769
                     /gene="pduO"
                     /locus_tag="ACHXWD_000381"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR7619406.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            10766..12151
                     /gene="pduP"
                     /locus_tag="ACHXWD_000382"
                     /pseudo
     CDS             10766..12151
                     /gene="pduP"
                     /locus_tag="ACHXWD_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            12162..13274
                     /locus_tag="ACHXWD_000383"
     CDS             12162..13274
                     /locus_tag="ACHXWD_000383"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR7619407.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            13271..14616
                     /locus_tag="ACHXWD_000384"
                     /pseudo
     CDS             13271..14616
                     /locus_tag="ACHXWD_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            14619..15173
                     /gene="pduT"
                     /locus_tag="ACHXWD_000385"
     CDS             14619..15173
                     /gene="pduT"
                     /locus_tag="ACHXWD_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR7619408.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            15173..15523
                     /gene="pduU"
                     /locus_tag="ACHXWD_000386"
     CDS             15173..15523
                     /gene="pduU"
                     /locus_tag="ACHXWD_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR7619409.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            15528..15971
                     /locus_tag="ACHXWD_000387"
     CDS             15528..15971
                     /locus_tag="ACHXWD_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR7619410.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(16072..16401)
                     /locus_tag="ACHXWD_000388"
     CDS             complement(16072..16401)
                     /locus_tag="ACHXWD_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR7619411.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(16573..17631)
                     /locus_tag="ACHXWD_000389"
     CDS             complement(16573..17631)
                     /locus_tag="ACHXWD_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR7619412.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(17829..18302)
                     /gene="sbmC"
                     /locus_tag="ACHXWD_000390"
     CDS             complement(17829..18302)
                     /gene="sbmC"
                     /locus_tag="ACHXWD_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR7619413.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(18421..19587)
                     /gene="dacD"
                     /locus_tag="ACHXWD_000391"
     CDS             complement(18421..19587)
                     /gene="dacD"
                     /locus_tag="ACHXWD_000391"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR7619414.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            19796..21223
                     /gene="sbcB"
                     /locus_tag="ACHXWD_000392"
     CDS             19796..21223
                     /gene="sbcB"
                     /locus_tag="ACHXWD_000392"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR7619415.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(21266..21493)
                     /gene="tsuB"
                     /locus_tag="ACHXWD_000393"
     CDS             complement(21266..21493)
                     /gene="tsuB"
                     /locus_tag="ACHXWD_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR7619416.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(21507..22565)
                     /gene="tsuA"
                     /locus_tag="ACHXWD_000394"
     CDS             complement(21507..22565)
                     /gene="tsuA"
                     /locus_tag="ACHXWD_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR7619417.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(22744..24102)
                     /gene="plaP"
                     /locus_tag="ACHXWD_000395"
     CDS             complement(22744..24102)
                     /gene="plaP"
                     /locus_tag="ACHXWD_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR7619418.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(24092..24154)
                     /gene="yoeI"
                     /locus_tag="ACHXWD_000396"
     CDS             complement(24092..24154)
                     /gene="yoeI"
                     /locus_tag="ACHXWD_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR7619419.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(24369..25298)
                     /locus_tag="ACHXWD_000397"
     CDS             complement(24369..25298)
                     /locus_tag="ACHXWD_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7619420.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(25344..26168)
                     /locus_tag="ACHXWD_000398"
     CDS             complement(25344..26168)
                     /locus_tag="ACHXWD_000398"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7619421.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(26251..26505)
                     /gene="yoeB"
                     /locus_tag="ACHXWD_000399"
     CDS             complement(26251..26505)
                     /gene="yoeB"
                     /locus_tag="ACHXWD_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR7619422.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(26502..26753)
                     /gene="yefM"
                     /locus_tag="ACHXWD_000400"
     CDS             complement(26502..26753)
                     /gene="yefM"
                     /locus_tag="ACHXWD_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR7619423.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            27036..27086
                     /gene="hisL"
                     /locus_tag="ACHXWD_000401"
     CDS             27036..27086
                     /gene="hisL"
                     /locus_tag="ACHXWD_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR7619424.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    27062..27185
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            27232..28131
                     /gene="hisG"
                     /locus_tag="ACHXWD_000402"
     CDS             27232..28131
                     /gene="hisG"
                     /locus_tag="ACHXWD_000402"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR7619425.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            28137..29441
                     /gene="hisD"
                     /locus_tag="ACHXWD_000403"
     CDS             28137..29441
                     /gene="hisD"
                     /locus_tag="ACHXWD_000403"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR7619426.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            29438..30508
                     /gene="hisC"
                     /locus_tag="ACHXWD_000404"
     CDS             29438..30508
                     /gene="hisC"
                     /locus_tag="ACHXWD_000404"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR7619427.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCESGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            30508..31575
                     /gene="hisB"
                     /locus_tag="ACHXWD_000405"
     CDS             30508..31575
                     /gene="hisB"
                     /locus_tag="ACHXWD_000405"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR7619428.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            31575..32165
                     /gene="hisH"
                     /locus_tag="ACHXWD_000406"
     CDS             31575..32165
                     /gene="hisH"
                     /locus_tag="ACHXWD_000406"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR7619429.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            32165..32902
                     /gene="hisA"
                     /locus_tag="ACHXWD_000407"
     CDS             32165..32902
                     /gene="hisA"
                     /locus_tag="ACHXWD_000407"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR7619430.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            32884..33660
                     /gene="hisF"
                     /locus_tag="ACHXWD_000408"
     CDS             32884..33660
                     /gene="hisF"
                     /locus_tag="ACHXWD_000408"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR7619431.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            33654..34265
                     /gene="hisIE"
                     /locus_tag="ACHXWD_000409"
     CDS             33654..34265
                     /gene="hisIE"
                     /locus_tag="ACHXWD_000409"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR7619432.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(34362..35339)
                     /gene="wzzB"
                     /locus_tag="ACHXWD_000410"
     CDS             complement(34362..35339)
                     /gene="wzzB"
                     /locus_tag="ACHXWD_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR7619433.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLAQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(35485..36651)
                     /gene="ugd"
                     /locus_tag="ACHXWD_000411"
     CDS             complement(35485..36651)
                     /gene="ugd"
                     /locus_tag="ACHXWD_000411"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR7619434.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         9562 a         9528 c         9082 g         8596 t
ORIGIN      
        1 agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt aataagttcg
       61 gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt atttcgttat
      121 tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat gagatagcga
      181 taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac aatgaaataa
      241 ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca ggtgaaaatc
      301 ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc ggtagaagca
      361 ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt gggtacgcca
      421 ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat gaccacttcg
      481 accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa gatactggcg
      541 agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa atcggtaaac
      601 agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc taattgcgcc
      661 agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag agcaatggta
      721 actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc aagcgaaatc
      781 cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc aactttcagg
      841 gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa ctcggcaacg
      901 cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt ttctttatca
      961 gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt gctctgcaga
     1021 aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata acaaattatt
     1081 gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa ttcccaccaa
     1141 acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat atcacagttc
     1201 cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag ccaacgcaat
     1261 taattcacat tgaaatatcc acaaaacaac caataaatta acaacatata gttcataaaa
     1321 gcaattttat aatatttgtt ctctattaat ttctctcatt agccgattaa gtcagccatt
     1381 ctttttcctc gctttttttt atagtcatat cgtcaaaaaa atccaggcga ggtctttatg
     1441 caacaagaag cgttaggaat ggtagaaacc aaaggcttaa ctgccgccat agaggccgca
     1501 gacgccatgg tgaagtcggc aaatgttttg ctggtcggct acgaaaaaat aggttccggg
     1561 ctggtgaccg tcatcgttcg tggtgatgtc ggggcggtaa aagcggcaac agacgcgggt
     1621 gccgcagctg cgcataacgt cggcgaagta aaagccgtac acgttattcc ccgcccacat
     1681 accgatgtcg aaaaaatctt accaaaggga attaacccat gagcagcaat gaactggtcg
     1741 aacagatcat ggcgcaggtc atcgctcgcg tagcgacgcc agagcatgag gttctcccga
     1801 aaaccactca tcaaatacga gagacggcta tggcagaaaa aagctgcacg ctaacagaat
     1861 ttgttggcac cgctattggc gacactgtag gtctggtcat tgcgaacgtt gatagcgccc
     1921 tgttggacgc gatgaaactt gaaaagcgct atcgttccat tggcattctg ggtgcgcgta
     1981 ttggcgcagg gccacacatt atggccgctg atgaagcggt aaaagccacc aacactgaag
     2041 ttattagcat tgaacttcct cgcgatacta aaggcggtgc cggacacggc tcactgatcc
     2101 tcttaggtgg caacgatgtt tccgacgtga aacgcggtat tgaagtcgcc ctgaaagaac
     2161 tcgatcggac ctttggcgat gtctatgcca acgaagccgg acatcttgaa ctgcaatata
     2221 ccgcccgcgc cagctatgca ctggaaaaag cgtttggtgc accactggga cgggcttgcg
     2281 gcgttattgt cggcgctccg gctgctgtgg gtgttctgat ggccgacacc gcgctgaagt
     2341 ccgccaatgt ggatgttgtg gcatacagct ccccagctca agacaccagc tacagcaatg
     2401 aagtcattct gattatctcg ggtgactccg gcgcagtccg ccaggcggtc atctccgcac
     2461 gcgaaatcgg taaaaccgtg ctttcgactc tcggcgatac accgaaaaac gatcaccctt
     2521 cctacatctg aattctctga ggcagattta tgagatcgaa aagatttgaa gcactggcaa
     2581 aacgcccggt caaccaggac ggtttcgtta aagagtggat tgaagaaggc tttattgcga
     2641 tggaaagccc gaacgatcct aaaccgtcaa tcaaaattgt taacggcgtc gttaccgaac
     2701 tggatggaaa acccgccagc cagtttgacc tgatcgacca ctttatcgcc cgctacggca
     2761 tcaatctcgc gcgcgccgaa gaagtcatcg cgatggattc ggtaaaactt gccaatatgt
     2821 tgtgcgatcc aaatgtcaaa cgtagcgata tcgttcccct taccacagca atgacgcctg
     2881 caaaaatcgt cgaagtggtt tcgcagatga acgtggtaga gatgatgatg gcgatgcaga
     2941 aaatgcgcgc ccgtcgaaca ccatcacaac aggctcacgt caccaacgtc aaagataacc
     3001 cagtacaaat tgccgccgac gcagctgaag gcgcatggcg cgggtttgat gaacaggaaa
     3061 ccaccgttgc cgttgctcgc tatgcaccgt tcaacgccat cgccctgctg gtcggctcac
     3121 aggtgggacg tccgggcgtc ttgacccagt gttcactgga agaagccacc gaactgaaac
     3181 tcggtatgtt aggccacacc tgttacgccg aaacaatttc tgtctatggt acagaacctg
     3241 ttttcaccaa tggtgatgat accccgtggt caaaagggtt tctcgcgtca tcctatgctt
     3301 ctcgtggctt aaaaatgcgc tttacttccg gctccggctc tgaagtacag atgggttacg
     3361 cggaaggcaa atcaatgctt tacctcgagg ctcgttgcat ttacatcacc aaagccgcag
     3421 gcgttcaggg tctgcagaat ggttcggtaa gctgcatcgg tgttccatca gctgtgccat
     3481 caggcatccg cgcagtatta gcggaaaact taatttgctc atcgctggat ctggaatgcg
     3541 cctccagtaa tgaccagacc tttacccact cggatatgcg ccgtaccgca cgtttcctga
     3601 tgcaatttct gccaggcacc gactttatct cttccggttt ttccgctgtg ccgaactacg
     3661 acaacatgtt tgctggttca aacgaagatg ctgaagactt cgatgattac aacgtcattc
     3721 agcgtgacct gaaggttgat ggtgggctgc gaccagtacg cgaagaagat gttatcgcta
     3781 tccgcaataa agccgctcgc gctttacagg ctgtctttgc cggtatggga ctaccgccta
     3841 ttaccgatga agaagtcgaa gcggcaacct atgcccacgg ttcaaaagat atgcctgagc
     3901 gcaatattgt ggaagacatc aagttcgccc aggagattat caataaaaac cggaacagcc
     3961 tggaagtggt gaaagccttg gcgcaaggca gttttaccga tgtcgcccag gacatgctca
     4021 acatgcaaaa agcaaaactg accggcgatt atctccatac ctcagccatc atcgtcgatg
     4081 acggacaagt gctctctgcg gtcaatgacg tcaatgatta cgccggaccg gctacaggtt
     4141 accgcctgca aggggaacgc tgggaagaga tcaaaaatat tcccggtgca cttgatccca
     4201 acgaaattga ctaagggtga gaaatggaaa ttaacgaaaa attactgcgt cagattatcg
     4261 aagacgtgct ggcagaaatg caacccagcg acaagtccgt ttcatttcgc gcacctgtct
     4321 ctgcaactgt cccttccgcg ccagataccg gaaattttct gaccgaaatt ggcgaagccc
     4381 aacaaggcac tcagcaagat gaagtcatta ttgctgtagg cccggcgttc ggcctggcgc
     4441 aaaccgtcaa tatcatcggt attccgcata aaaacatctt gcgcgaagtg attgccggta
     4501 tcgaagaaga aggcattaaa gcccgtgtaa ttcgctgctt taaatcgtcc gatgtcgcat
     4561 tcgtagcagt cgaaggcgat cgtcttagtg gttcaggtat tgctatcggt attcagtcga
     4621 aaggcacaac ggttatccac cagcagggac ttcctccctt gtctaacctg gaacttttcc
     4681 cacaagcgcc tttgttgacg ctggatactt atcgtcagat tggcaaaaac gccgcgcgtt
     4741 atgcgaagcg cgagtctcca caaccggtgc cgaccctcaa cgatcaaatg gcgcggccga
     4801 aataccaggc caaatctgcc attttgcata ttaaagagac gaagtacgtg gtcactggca
     4861 aaaaaccgca agaactgcgc gtgacgtttt aacaaaggaa accttcatga ataccgatgc
     4921 aattgagtcg atggtcaggg acgtgttgaa ccgcatgaac agtttacagg atgcagctcc
     4981 tgtttctgcg gtccccaacg catcaatact gagcgccaaa gtgacggatt acccgctggc
     5041 aaataaacat ccggaatggg ttaaaacagc gaccaataaa acgctcgacg atttcactct
     5101 ggaaaatgtg ctaagcgata acgtcaccgc cctggatatg cgcattaccc ctgaaacatt
     5161 gcgtattcag gctgcgattg ccagagatgc tggtcgcgat cgtctggcga tgaattttga
     5221 acgcgccgct gagcttacct cagtaccaga tgatcgcatc ctcgaaatct acaatgcgtt
     5281 acgcccttat cgttcaacta agcaagaact tattgccatt gctgacgatc ttgaacagcg
     5341 ttatcaggcc aaaatttgtg ctgcatttgt ccgtgaagct gcggaacttt atgtcgagcg
     5401 caaaaaactg aaaggcgacg attaaggacg aactatgcga tatatagcag gcattgatat
     5461 cggcaactca tcaacagagg ttgcgctggc aacgctcagt gcaactggag agttgtcgtt
     5521 cgtgagtagc gccctggagg aaaccaccgg aatcaaaggt acgttgcgta acgtccatgg
     5581 tattcaggag gcgctcgcac aggcaacgaa aaaagtcggc atcaatgtta gcgatatttc
     5641 gcttattcgt atcaacgaag caactccggt cattggtgat gtggcgatgg aaaccatcac
     5701 tgaaactatc atcaccgaat caaccatgat cggtcacaac ccgaaaactc cgggcggagt
     5761 ggggctgggc gtagggttca ccatcactcc gcaagagtta ttaacacgcc cggcggacac
     5821 accttacatc ctggtggtgt catcggcatt tgattttgcc gatatcgcga cgatgataaa
     5881 cgcctccgta cgcgccggat atcagctgac tggcgttatt ctgcaacgcg acgatggcgt
     5941 actggtcaat aaccgcctgg aaattccttt acctattgtg gatgaagtcc tctacatcga
     6001 tcgtattccg ctcggcatgc tggcagcaat tgaagttgcc gttcccggga aagttatcga
     6061 aaccctgtct aacccgtatg ggattgccac cgtattcgcc ctcaatgctg aagagactaa
     6121 aaatattgtt cctgtcgcgc gggcgctaat cggcaaccgt tcagcagtgg tagtgaaaac
     6181 gccctctggc gacgttaaag cccgttcgat acccgccgga aatatcgaac tgctttctgc
     6241 cgggcgcaca acgcgcgttg atgtagctgc tggtgccgat gccattatga aagcggtcgg
     6301 tgagtgccca aaactggaaa acgtgacggg agaaccaggc accaacatcg gcggaatgct
     6361 ggaacatgtt cgccagacca tggcggaact gaccaacaaa cccagcaacg aaatttttat
     6421 tcaggatttg ctggcgatag atacctctgt ccccgtcagc gtaaccggtg gtctggcagg
     6481 tgaattttca ctggaacagg ctgtgggtat tgcctctatg gtgaaatccg accgtctgca
     6541 aatggcgatg atcgccagtg aaattaagca aaaactgcat gttgatgttc aggtcggagg
     6601 cgcagaagca gaagccgcca ttcagggggc cttgactacc cctggaacca cacgtccact
     6661 ggcaattctg gacttaggtg caggctctac cgatgcctcc atcatcaatc agagtgggga
     6721 aatcgttgcc actcaccttg cgggagcggg cgacatggta acgatgatta tcgctcgtga
     6781 gctgggctta aatgatcgtt atctggcaga agaaatcaaa aagtacccgt tagcaaaagt
     6841 agaaagcctg ttccacttac gccatgaaga cggtagcgtt cagttcttcc ctactcccct
     6901 atccccccat gtatttgccc gcgtctgtgt agtgaaaccc gacgaactgg tgcctattcc
     6961 cggtgatttg acgctggaaa aagtacgcgc tgttcgtcgc agtgcgaaag agcgtgtctt
     7021 cgtaaccaat gccctgcgcg cgctacgtca ggtaagccct gccggaaata ttcgcgacat
     7081 tccgttcgtc gttctggtgg gtggttcttc gctggatttc gaagtaccgc aattggtcac
     7141 cgacgcgctg gcacattatc gactggtcgc cgggcggggg aatatccgtg gtagcgaagg
     7201 tccgcgcaac gcggtagcta ctgggctact catcgcctgg cacaaggagt cgattcatgg
     7261 aaagtaaaga gcgtgcacca gccatcgttg tcatggagat cggtgactgt atcactacat
     7321 gggatgaggt gcttcttggc attgaagagg aaggtattcc ctttcgcatt caacacattc
     7381 cgtcaggtga agttatcgat agcgcctggc tggctgctcg tcaatctccc ctgttggttg
     7441 gcattgcctg cgaccgggaa gaactgattg tgcattacaa aaacttaccg gcatcagcgc
     7501 cactgtttac gctgacgtat caacaagata accatacccg gcgcagcatc ggcactaacg
     7561 ctgcccgcct ggtaaaagga attcccttcc gggaattcca ctcataatcc acaggagaaa
     7621 cgctgtatga acaacgcact aggactggtt gaaacaaaag ggttagttgg cgcaattgaa
     7681 gctgcagatg ccatggttaa atccgccaac gtgcagctgg ttggctatga aaagattggt
     7741 tcgggtctga ttacggtgat ggtccgcggt gatgttggcg cagtaaaggc cgccgttgat
     7801 gcggggagcg ctgcggcaag cgccgttggc gaagtgaaat cctgccatgt catcccgcgc
     7861 ccacacagtg atgttgaggc tattttacct aaatcggcat aaccccattt gctaaggagc
     7921 acagcgtgaa gcaatcactg ggattacttg aagccagtgg tctggctctc gccatcagtt
     7981 gcgcagacgc tatggcaaaa gccgcgtcca tcacgctggt tggccttgag aagaccaatg
     8041 gctcaggctg gacgctcatc aaaatcaccg gtgacgtcgc ctcggttaat gcggcgatcg
     8101 cgacgggagc cagtttcgcc ggacagcgaa atggccttgt cgcacacaaa gtgattgccc
     8161 gcccgggaga cggcattctg ccccacacaa ctgaagcatt gaccgtgacg gttgaaacaa
     8221 gcaaaacacc agcaacaacc gtcacctcag agattatcag ttgtaatttg tgtcttgacc
     8281 cggcatgtca gcggcaaaaa ggtgagcccc gctcactttg cctgtacgcc aataagcgag
     8341 gggagtagca gtggataaac aaaccttgca ggaaacagtg agccagattc tggatgagat
     8401 gcgcgagcgc cctatcccac tgggcgtttc cagtcgccat atacatcttt catcagcgga
     8461 ttatttgcgt ctttttcctg aacaaccgat tagcgagaaa aagccgttaa tgcaacctgg
     8521 ccaatacgct gctgaacagg ttgttacgct ggagggacca aaaggcaaat tgcacaacgt
     8581 tcgcttgttg gggccgctac gcaatgcaag ccaggtggaa atttcccgga cggacgccag
     8641 aacgttaggg atcaccgccc cgctacgcat gtcaggcgat ctcagcggca ctccggggat
     8701 tcgtttgata agcccatttg cggaactgga gatcacatct ggcgtcattg tcgcgcaacg
     8761 gcatattcat atgtctccgc tggatgccct gatttatggc gttacgcatg gcgataaagt
     8821 ggcggttgcc attgaaggtg atgagcgacg actgatattc gaaaacgtcg ctgttcgcgt
     8881 ctctccggat atgcgtcttg aaatgcatat tgataccgat gaggccaacg ccgcaggcgc
     8941 tgatagccct gacgtttttg ccacactggt gtcagtgcca tgaataacat gctgatgcaa
     9001 cgtattgttg acgaagtggt ttttcgcctt aaacaacgtg cgggaaaaac actcgtgctg
     9061 actgtttttc aattgcgaga cgccagcgtt caagagagcg ttcatcagta tgcgtcatta
     9121 caaatccgat atgtcgatct gccgctgctg cgacaacttg cggagaacga aacgtcagat
     9181 cgagcatcta tccagataca cgaagcgtta gcctggggaa tgcacattca attgtcgttg
     9241 cagcgtcatt tcctgaacgc catcgaattg aagactctcg cccgtctacc gctaagctgg
     9301 tgcgacgaac aagggcagcc gatctacctt catcataatc gacttttaag ttatgccgat
     9361 atcgcacagt taagctcggg aatactggtg ttacagcgga aatgttgtgt gaccgcactg
     9421 gctcgtgaag cggcaattac ccgaaatatt cagttaatca ggcaggagta acctatgcat
     9481 ctggctcgag tcactggcgc ggtagtatcc acgcaaaaat caccttctct gatcgggaaa
     9541 aaactgctgc tggttcgccg ggtcagtgct gacggcgaac tgcctctcac gccattaacc
     9601 ggcgacgaag ttgccgttga ttcagtcggc gcaggcgttg gtgagttggt actcctcagc
     9661 agtggttcca gcgccagaca tgtgttttca ggccctaacg aggccatcga cctggcagtg
     9721 gtcggcattg tcgatacgct ttcgcgctaa ggagctaaaa gatggcaatt tatacccgaa
     9781 caggtgattc aggaagtacg tctttattta ccggacagcg cgtcagcaaa actcatctac
     9841 gtgtcgaaac ctatggcacg ctggatgaac tgaacgcgac actcagtctg tgttattgcg
     9901 ccaccgccat cgaaagccat cgcatcttac tggaagccat tcaacaacag atattttggt
     9961 ttagcgccga actcgccagt gaaagcgaac agccgtcagc gcaacagcgt tacatcggta
    10021 cggaagaaat tgccgcgttg gaaaacgcga tcgatagtgc aatgaacgcc gtccccccag
    10081 ttcacagctt cattctgcct ggtcgatgtg aagccgcaag ccgcatgcat ttcgcccgca
    10141 cggtggctcg acgggctgaa agacggctgg ttgaactgac aacggaaacc accgtccgga
    10201 atgttttgct gcactatatc aaccgacttt ccgattgcct gtatgccctc gcccgcgtgg
    10261 aagacaacgt tgctcatcaa aacctgatga ttcaggaaat cacaaaacgt tatcacgaag
    10321 ccaaccacac accagcattg aaggaacgca caatgccgct cacttttcag gatcttcacc
    10381 agctcattcg tagtgcggca atgcgcgcgg atgaactgca tattcccgtt gtcatcagca
    10441 ttgttgatgc caacggaaca gaaagtgtta cctggcggat gcctgatgcc ctgttggtca
    10501 gcagtgaact ggcaccgaaa aaagcctgga ccgccgtggc gatgaaaacg gcaacccaca
    10561 agcttgctga taccgtgcaa cccgacgcgc cgctttacgg gctcgaaagc catatgcaag
    10621 gaaaagtcgt cacttttggt ggcggcttcc ccctctggcg tgacggaaaa ttgcttggcg
    10681 ggcttggcat cagcggcggt agcgttgaac aagacatgga tattgcccaa agcgcaatgg
    10741 cggcaattaa cgtgggagta aaccaatgaa taactctgaa ttagaaacac tgatccgcaa
    10801 tattctgcgt gagcaacttg tccccgctac accacgaacg caaagaaacg ctattttcca
    10861 gactgtcgat gaagcggtct gcgcagcaca ctaggccttt ttacgctttc aacagtgccc
    10921 gcttaaaacc cgtagcgcca ttatcagcgc cattcgcgaa gaattaatgc cccgccttac
    10981 cgaactggca gaagaaagcg ccaaagaaac ggggatggga aataaagaag ataagtacct
    11041 aaaaaacaaa gcggcactcg ataacacgcc aggaattgaa gatttaacca ccacagcact
    11101 aaccggtgat ggtggcatgg ttttgtttga gtactctccg tttggggtga ttggctcggt
    11161 cacgccaagc accaacccca cggaaaccat cattaacaac agcatcagca tgctggcagc
    11221 aggtaatagc gtctacttca gtccacaccc gggtgccaaa aacgtttcgt taaaactgat
    11281 taccacaatc gaagacatcg cgtttcgctg tagtggcatt cataacttga tcgttaccgt
    11341 atctgaaccg acctttgctg ccaccgaaca gatgatggcg caccctaaaa tcagcgttct
    11401 ggcgattacc ggcggtccgg ggatcgtcgc catgggaatg aaaagtggca agaaagtgat
    11461 tggtgccgga gcgggtaacc ctccgtgcat cgtcgatgaa acggcggata ttgtgaaagc
    11521 cgccgaggac atcatcaacg gcgcttcatt tgattacaac ctgccgtgta ttgctgaaaa
    11581 aagcctgatt gtcgtggcat cagtggcaga ttatttaatt cagcagatgc aaagttttgg
    11641 cgcacttctt cttaactatg agcaaactga gaaattgcgt gcaatttgcc tgccagacgg
    11701 tagcgccaac aaaaaactgg ttgggaaaag cccatcagcc ttgctggaag cggcaggcct
    11761 ccctttgccc gcaaaagcgc cgcgcctact gattgctgtt gtcgacgccg atgatagttg
    11821 ggttacttgt gagcaactaa tgccaatgct gcccattgtg aaggtcaagg attttgacag
    11881 cgctctaacc ctggcgctga aagtggaaga cggcttgcac cacacggcaa ttatgcactc
    11941 tcagaacgtc tcacgactca accttgccgc gcgcgttatg caaacctcaa tatttgtcaa
    12001 aaacggcccc tcctgggcgg gtattggcgt cggcggcgaa ggctttacga cctttaccat
    12061 tgcaacaccg actggagagg gtacaacgtc agcacggact tttgcccgct cacgacgctg
    12121 cgtgttaacc agcggctttt caattcgcta gagaaacgga aatgaatact ttttcactgc
    12181 agacgcgttt atacagcgga ccaggaagtc tcaaggtact tagccgtttc tctcatagac
    12241 acatctggat tatttgcgat gcctttctgg cacgctcacc acttattgac acgttgcatc
    12301 aggcgctgcc cgacagtaat cgtctcagta tttttagcga aataacgccc gccccaacta
    12361 tccagaccgt ggtgaaaggc attgcgcaaa tgcagacttt acgcccggat gtagttatcg
    12421 gttttggcgg cggttctgcc ctggacgccg caaaagcaat tgtctggttc ggtcgccagt
    12481 gtggcattga aattgaaacc tgcgtagcca ttcctaccac cagcggtatc ggttccgaag
    12541 tgaccagtgc ttgtgtcatc agcgatcctg agaaagggat taaatatccc cttttcgata
    12601 atgcacttta tccggatatc gcgatccttg atccatcttt ggtcgtcagc gtgccaccgg
    12661 cgataacggc caataccggg atggatgttt taacccatgc gcttgaagct tatgtttctc
    12721 ctcgcgccag cgacttcacg gatgcactgg tcgaaaaagc cgtgcaaatt gtttttcaat
    12781 acctgcccac tgcagtgaaa aaaggcgatt gtcttgccac ccgtggcaaa atgcataatg
    12841 catcgacgct tgcaggaatg gcctttagcc aggccggact cggtttaaac catgcaattg
    12901 ctcatcaact cggcggacag tttcatctgc cgcatggact ggcgaatgcg ctcctgctca
    12961 cggcagttat ccgctttaat gctggcgatc cgcgcacagc caaacgctat gcccggctgg
    13021 cgaaaacgtg ccatttatgc ccggacaacg ccaatgacac cgccagcctg aatgcactta
    13081 ttcaacatat tgagcagctt aatacaacct gtacgctgcc gactcttgcc aatgcgctga
    13141 aagaaaaaaa agcagaatgg tcaatacgca taccagatat ggttcaggcg gcgctggcgg
    13201 atgcaacgtt gcgtaccaac ccgcgtgcag ctgatgcctc cgcgattgca gaactgctca
    13261 aggggttgtt atgaacgtcg gattaatctc ctgcgacgac cagaccatcc gcgagcgagt
    13321 acaagcagca ggtgtcgtgg gtgctggcgg cgcaggtttt ccaacccata ttaagctaca
    13381 agcgcgtgct gacactttcc tggtgaatgc ggcggaatgc gaacccatgt tgaaagtaga
    13441 tcaacagtta atggcgcagc aagccagccg cctgcttcgc ggtgttcact atgcgatgaa
    13501 agccaccggg gccagtagcg gaatcatcgc gctgaaagaa aaatatcaac gggcaataaa
    13561 cgcactcaca ccactgttac caccggatat ccgcctgcat attttgcccg acgtttaccc
    13621 cgcaggagac gaagtgctga ccatctggat ggcaactggt cgccgcgttc cacccgctgc
    13681 attacccgtt agcgtcggtg tggtggtcaa taatgttcaa accgtactta atattgccag
    13741 agccgtcgag caacagtatc cagtgacgcg ccgcacactc accgttaatg gtgcagtggc
    13801 gaagccaata acggtaaccg tccccatcgg catgtcttta cgtgaagtcc tggcgctggc
    13861 gggcggcgca acagtggatg ctcctggttt tatcaatggc gggccgatga tgggaagttt
    13921 gatcacatcg ctcgacacac cagtcagcaa aaccactggc ggcttgctgg ttttacccaa
    13981 ctctcattca ctgatccaac gtcgaatgca gaatgaccgc agcgtacttg ccgtcgcgaa
    14041 aactgtttgt gagcaatgcc gcttgtgtac cgatctctgc ccacgtcatt taatcggtca
    14101 cgaactggca ccccatttat tagtacgtgc gcggttaact accaacagct cgccacgccg
    14161 caactgttac tgacagcgct cacctgttca gagtgcaacg tctgcgccag tgtcgcctgt
    14221 ccggtaggaa tttcaccaat gcgcataaac cgcctgctta agcaggaatt acgcgcacaa
    14281 aatctccgct acgaaggtgc cttaaatcct gcagatccca tggcaaatta tcgactgatt
    14341 cccgtaaaac ggctggtaac cagacttggc ttaaccccgt ggtatcagga tgcgccattg
    14401 tctgaacagg tgcctcagcc cgaaaaagtg acgttattgt tacgccagca catcggtgcc
    14461 agcgcaattg cgtgtgtaca aaaaggcgat cgcgtcgttc atgggcaatg tgtaggtcaa
    14521 attccacacg gcacattagg tgcccccatt catgccagta ttgatggcat ggtcagcgat
    14581 gtgacagaaa acgcgattac gcttgtgaga ggttaaccat gtcgaaagcg gtaggtattt
    14641 tagaacttag cagtatcgcg aaagggatgg aaaccggcga cgccatgctg aaaagcgcca
    14701 acgttgagtt actggtcagc aaaacgctat gccccggaaa atttctgctg atgctcggtg
    14761 gcgatgtggg cgcggtgcaa caggccatca acagcggagc gtcccttgct ggtgaaatgc
    14821 tggtcgacag tcttgtcctg ccgaatattc atcccagcct cctttctgca atcagcggac
    14881 ttaaccatgt tgaacagcac cgggctgtcg gcgtagtcga aacctggagt gtcgccgcct
    14941 gcattgacgc tgccgattgt gctgtcaaag ctgcaaatgt cattcttgtt cgcgtacata
    15001 tggcttttgg cattggcggt aaatgctaca tggtggttgc tggcgatatt tcagatgtta
    15061 ataacgcggt ctctgccgcc agcgtgcgcg cgggggaaaa aggtttgttg gtctatcggt
    15121 cggtgattgc ccgtccacat gaagccatgt ggcgtcagat ggtggaggga taatgacaac
    15181 tcagcacaca actgaacgga tgattcagga atatgtccca ggtaagcagg tcacgctggc
    15241 acatgtcatc gccaatccag ggaaggatct gtttaagaaa ttgggtttac cagacaccat
    15301 ttccgctatt ggtattctga caattacccc cagcgaagca tctatcatcg catgcgatat
    15361 cgccacaaaa tcaggcgctg tagagatcgg ttttcttgat cgttttaccg gtgccgtagt
    15421 gctgactggt gatgtttctg cgatcgaata tgcacttagc caggttaccc gtacgctggg
    15481 tgaaatgatg cgctttaccg cctgccccat tacgcggacc tgacgttatg caacgtatga
    15541 tgctcatcgg ccccagccag tgcggaaaaa cgtcactcac gcaatgtctg cgtggcgaaa
    15601 atattcatta tcagaaaacg caggcaatcg tctggtcccc gaccaccatt gatacaccag
    15661 gcgaatacct cgaaaaccgc agcctctaca gtgcactgtt ggtcagtgcc tgcgaagcgg
    15721 acattatcgc gctggtgctt aatgcggacg caccgtggtc acctttttcg ccaggcttta
    15781 cctcgtcaat gaatcgcccg gtcattggag tagtaaccaa agcagattta gccaacatgg
    15841 aacaaatatc tttggtgaaa tgttggttac gggaagctgg agcgcacaac gtgttagtaa
    15901 ccagtgcggt taataaccat ggagttgcag aacttttagc cttgctgcat acagaagaag
    15961 gctgtcgcta atttcgacag gttatcagat gggaatgatg acggtgatac acagaaaaga
    16021 atcaatgagc aatgagggtt gccgggcaac cctcattgaa taaaacagga attacttcgc
    16081 ttcgccgttt ttcatttcgc ccatagcttt cagctttttg gagatatcgc ggcgttcttt
    16141 ggagagctcg gcatttttga tgatgtaatc gtcaacgcga tcttcatagt cacctttcat
    16201 gctggcgatg atgccctgga ttgcttcaac gctcatccca ggcttgatgt aatcgctcag
    16261 gttgtccagc agcaggacgc gcttctggtt gtcacggatc tttttctcaa cgtcctgaat
    16321 ttcacgttgc agtttgttct tacgacggaa cagacgaaca aattccagta cgtcctgaaa
    16381 tgaaggcttg gtagtttcca tttttatacc cctgataatg tgagagtcgg attcgtttaa
    16441 gcaaccgcta ttcgttaggg ccaacattac tcatagctgc cgcagatgac aatgctttta
    16501 tcccttttca ctatcatacc ctttatcctt gctgaatcga agcagcagca agatgattct
    16561 gaagttcagg aattatttgc gcaaggcccg gcaaatcaga ttcgatagca actcaagctg
    16621 atgcgccgtt tccatgttca gccacacata accatagatt ggtgtttcca ctaccttcag
    16681 gtcatggtgg ttattgagca actgacgcag ctcttccaca gcatcgttca atttttccgt
    16741 attggcaaaa accggctgcg gattaccttc gtacagcgca tgaacaaagc tcagcagtat
    16801 ttgctgcatc atgtgctggg tatcacgaag tttttgcgcg ttcaataaca cgaaatggct
    16861 ggggcgcgtg gcccagtacg cattgatttg caactccagc atacaaacca gattgcggtt
    16921 aatggtctgg ataccttcat atatcgattt tggaatacgg gtttctttgc tggcgggcgc
    16981 aatcagccca cgcattttca cggcatcggt caggagtttt tgtagatggc tttccagacg
    17041 tgggcgttcg agtaagttcg gtgagaatgc agattgatag acccgattat actcggtcag
    17101 acttttcgcc agttgaatgc gccagtggat gaacgcccgt tgtggccaga taccggtaaa
    17161 caacattgcc agtaaagagc cgaggatcac atcgccgctt cgccataacg ccgtatcaat
    17221 ttcacctgtc ggggaaccaa caacaattgc cagcgtcacc ccaatcaata atccttgata
    17281 cggtttcttg cccagcgcca gccaaccgca aagaaacatc gccgccgcgc accagactaa
    17341 catcagcggt aacgaaatta actccagttg cagagcgata agacctaaaa tcgaacccaa
    17401 caccgtaccg ccaatacgtt cgaaggcacg agggacaacg ttaccccaga acgagattgg
    17461 ccccataatc accaccatgg tgaccagcgg ccaggtgctt tccgggatag taaacaggcg
    17521 gataatgaga aaagtgagca ggaatgccag cgcgacccga gtaccatgca ctatgcggta
    17581 atggcggtat acccggattt caaacgggct taatgactta tcggcacgca cgcagaaact
    17641 ccgtaatgaa gattaaaagc gctcataact catctgtcgg ccagaaggcg cgcctctgaa
    17701 ttatccgacg gacaaaccgg gaacagtgta atttcccggc aggagcgaaa aaattgaaag
    17761 gcgcatctta ctcttttcgc tttcaaaaaa agagtggtca tcgcgttaac acaccgccct
    17821 gagatgaatt agtgatgttt tggctgcacc gcaacataca tttcgatatc ccagtaccca
    17881 tcttccgcgc cattgttcaa ataaacctcg aagcagggct ttggtaacat ttcataagca
    17941 ctgtcctgca agaggctatt aaagaactga taccagggtt tagcaaaatc atcaccgact
    18001 acacgagcca ccgccaccgc atactgacca cctgaaattt ctgtcagaat gacgccctca
    18061 ctgttttcgg gaagcgtaaa gttattcggt accgtcacaa cggtgtcgca gcgtaatttt
    18121 tcggcgggtg tttcatctgg attgtcgtaa tagacagcaa cccactcctt cggcacaata
    18181 tttttgttat ctacccacat catcaactgc tcaaagcctt tctttaccgt ctgttcccac
    18241 gggccaacga gatggaaacc tgcaacggta cgtttctctt cctgcgtaat ctcgtagttc
    18301 atgacgcctc cattaacact gtttttatat acagcatagt tgcaatatta aaaccacaag
    18361 gaatgagtgt tgattatgcg agcagactcg cactcctgcc agtctgctgc aaaagaaagg
    18421 tcaggcctta tggtggaaat aatcactcag gcgggaaaac atgccgcctt ccccgacaga
    18481 ttccagggta accagcggcc agtgcgccac ctgtttatca cggtcgtaaa gttcaatttc
    18541 ccctacccgc tgatgggcgc taattggcgc ggtgagctct ttaccatcaa gggtatattt
    18601 ggctttgata tgtggaattt cggctttcgg tagcaccatc cagaactctt gttccgttcc
    18661 taggtcgata ttttctttat cgccatacca gatgcgttcc gttccgacct ttttcccacg
    18721 gtgcaaaatt tgcaccgtag taaagttttg ttgcccccaa cgcagtaatt ttcttgcctc
    18781 ttcctcacga ccttttgcac tgtcagcccc cattaccact gcaatgagac gacgctgccc
    18841 atctacagcc gaagcaatga gattaaaccc ggcaccagaa gtatgacccg ttttcaggcc
    18901 gtcaacattc atggttttat cccacaacaa cccgttacgg ttttgctggg tgataccgtt
    18961 ccaggtgaga cttttctcac tgtacatatg ataaaactcg ggctcgccgt ggatgatagc
    19021 gcgagaaaac acagctaaat cataagccga gctatgctgg ccaggtgcat ccagaccatg
    19081 cactgtttca aaatgcgtat ccttgagatg cagcttctcg gcatagttgt tcatcatttc
    19141 aacaaactgc cgttgcccac cggcaatata gtcagccaga gcaacacaag cgtcatttcc
    19201 ggaatccaca attaaaccac ggcttaaatc acgtaccgat acgcgatcgc cctctttcaa
    19261 aaacatcagt gaagaaccga caaacaccgg attatctttc gcccacgcat cgcgccccac
    19321 ggtgacaata tcgtctggcg taatgcgatg actatcgata gcgcgatcca cgacataacc
    19381 cgtcatcagc tttgtcaggc tggcgggatt gcgctgttga tgctcattac ccgcggtgag
    19441 gatctgaccg gtggtgtaat ccatcaatac ccaggacccg gcatgaatct ctggaggctg
    19501 aggtgaaaaa ggaatgtttt ccgccgcaaa accagacgat aagttaaaaa cgaacaaaga
    19561 agcagcaata ataagacggc gtttcaacag caaaccctca ggagtttcaa atagctgttc
    19621 tttttacgga aatacttatg aactggctgg aataaagtgc aagaaaatgt gactaccctc
    19681 tcatttttat ctgacatgat ctgttgccac tcgctgccaa attgtggcgc taaagctgat
    19741 tagcacggtg atatttgata ctctggcaga cagcagaaat aacggattta acctaatgat
    19801 gaatgacggt aagcaacaat ctaccttttt gtttcacgat tacgaaacct ttggcacgca
    19861 ccccgcgtta gatcgccctg cacagttcgc agccattcgc accgataacg aattcaatgt
    19921 catcggcgaa cccgaagtct tttactgcaa gcccgctgat gactatttac cccagccagg
    19981 agccgtatta attaccggta ttaccccgca ggaagcacgg gcgaaaggag aaaacgaagc
    20041 cgcgtttgcc gcccgtattc actcgctttt taccgtaccg aagacctgta ttctgggcta
    20101 caacaatgtg cgtttcgacg acgaagtcac acgcaacgtt ttttatcgta atttctacga
    20161 tccttacgcc tggagctggc agcatgataa ctcgcgctgg gatttgctgg atgttatgcg
    20221 tgcctgttat gccctgcgcc cggaaggaat aaactggcct gaaaatgatg acggtctacc
    20281 gagctttcgc cttgagcatt taaccaaagc gaatggaatt gaacatagca acgcccacga
    20341 tgcgatggct gatgtgtacg ccactattgc gatggcgaaa ctggtaaaaa cgcgtcagcc
    20401 acgtctgttt gattatctct ttacccatcg taataaacac aaactgatgg cgttgattga
    20461 tgttccgcag atgaaacccc tggttcacgt ttccggaatg tttggggcat ggcgcggcaa
    20521 taccagctgg gtggcaccgc tggcgtggca tccagaaaat cgcaatgccg taattatggt
    20581 ggatttggca ggagacattt cgccattact ggaactggat agcgacacat tgcgcgagcg
    20641 tttatatact gcaaaagccg atcttggcga taacgccgcc gttccggtta agctggtgca
    20701 tatcaataaa tgtccggtgc tggcccaggc gaatacgcta cgcccggaag atgccgaccg
    20761 actgggaatt aatcgtcagc attgcctcga taacctgaaa attctgcgtg aaaatccgca
    20821 agtgcgcgaa aaagtggtgg cgatattcgc ggaagccgaa ccgtttacgc cttcagataa
    20881 cgtggatgca cagctttata acggcttttt cagtgacgca gatcgtgcag caatgaaaat
    20941 tgtgctggaa accgagccgc gtaatttacc ggcactggat atcacttttg tcgataaacg
    21001 gattgaaaag ctgttgttca attatcgggc acgcaacttc ccggggacgc tggattatgc
    21061 cgagcagcaa cgctggctgg agcaccgccg ccaggtcttc acgcccgagt ttttacaagg
    21121 ttatgcagat gaattgcaga tgctggcaca acaatatgcc gataacaaag agaaagtggc
    21181 gctgttaaaa gcgctttggc agtacgcgga agagatcgtc taatttgaag cctccgccgc
    21241 tggtacggcg gaggtcaatg ttgagtcagg ctttttgaac ggtgatactc cacgctgcat
    21301 cgccaatctg ctgataatcg gtgatggcat gcccttcttc tgccgcccac tgtgggattg
    21361 cttcagttgc ctgggtgcaa tcaaactcaa ttaccaactc atcgccgctg gccatttctg
    21421 ccagtgctgc ttttgcttct atgagtggaa acggacagac ctgcgtcacc acatccagct
    21481 ttttaatcgc catctttaca ccttacttaa tttgccgcag cagttgccag tcgcgcctta
    21541 cgctgcggtc gaacatacac aagccaggac gcagtccaga ctccgagaat cataaataca
    21601 aggccaatcc agccctgcca ggtcatcatt gcagtcataa ccagtccatt accgataaag
    21661 caaccacctg caatactggc accgaagccc atcagtacac cacctaaccc gctacgtaat
    21721 gttgtttgtg catcagctgc gcgaacgcgg aactcacggc tcgctttggc tgcaataaat
    21781 gaccccacga agatccctaa caccaggaaa acgccccagt taatgtattt cacgtcaccc
    21841 gcgaccagaa attgcagaat attggccgtt ggggaagtga ttcctaaccc aaacatgcgt
    21901 ccggttgctt cactcagggg ccaggctaaa agcgcaatca aaccgatgag tacagcagtg
    21961 acaaagggat gccagcgttt ttcaaacaga atatgagcga tcccagttcg gcgcggcggt
    22021 aaggtcgcga ctttgagttt tggcttcttc aactctttca tcaccaccca gagcgttatc
    22081 accagcaaaa cggcaaccaa cggccaaaca gacaaattaa aagtctcagc aatagagtta
    22141 tgttcagtac tgtagtgctg caaggtttga tttaaaccac tggcatgtgg agaacgcatc
    22201 accgcactca tcaccatata agtgaaaagc gcgatccaac tgccgatcaa tccctcaccc
    22261 gcgcgatacc aggtcccggt ggcacatcca cccgccagaa caattcccag cccgaagata
    22321 tacccaccta taacagtacc aagccacggg aacgcctcgg cttcgtaagt cagtaggccc
    22381 gcctgaatta acgcaaaaac ccccacgctt tgtaccgaaa ttgcaatcag caaggcgtaa
    22441 aacatgcgat tatttttcgc gatatacata tcgcgaaaac cacctgtcag gcaaaaacgc
    22501 ccacgctgca tgacaaatcc cagcagagca ccacaaatta gcccgcttaa tatcattgaa
    22561 aacataaatt aattaaccag atgaatgtta atgaggaaat tattcatgac tggtggaatg
    22621 cagaccaata accaaattct ctaaattaga acaaatggtt attaatgagg atttcgataa
    22681 aaaaaaataa aaaaaccgga gccaaatatt cattcgactc cggttgatta ttagaaaaga
    22741 tgattatgca acgtcttcgt actgcggaac tgggttacgg aagctcttgg tgacgcaagc
    22801 caggtaaatc agaccgatag cagcccaaat cagacccaga accattgagc tttcttccag
    22861 gttaacccac agcgcaccaa cggtcagcgc accacacatc ggcaggaaca gatactggaa
    22921 gtgatctttc agcgtcttat tacgcttctc acggatccag aactgcgaga tgaccgacag
    22981 gttaacgaag gtgaacgcca ccaacgcacc aaagttaatc agcgctgtag ccattaccag
    23041 gtcgaagttg attgccagca gggcaatcgc gccaaccagg atgatgttca tcgccggagt
    23101 acgccatttc gggtgtacat aaccgaagaa gcttttcggg aatacgccgt cacgacccat
    23161 tacgtacatc agacgcgcta cgcctgcatg cgccgccata ccggacgcca gtacggtaat
    23221 ggtggagaag atcagcgcgc caacctggaa cgctttaccc gcaacgtaca gcatgatttc
    23281 aggctgtgac gcatccggat ctttaaagcg agagatatcc gggaagtaca gctgcaggaa
    23341 gtaagttgca aagatgaaga tcatgccgcc aatcagcgcg gtcaggaaaa tcgcacgcgg
    23401 gatcacgcgc tctgcatctt tggtttcttc cgacaggttg ctgatgccgt caaagccggt
    23461 aaaggaaaag cacaggattg tcgccccggt aatcatcgga ataacatgcg catcgccaga
    23521 ccagaacgga cgagtgctcg ccagcgtacc agcgccttca ccttcaaata cgccataaac
    23581 aaccatgccc agaatcaccg cgatcagcac tacctgcaac acgacgatta cagtgttgaa
    23641 gttcgctacg gatttcagac tacgcaggtt aaaggcggtc ataaaggcca ccagcgccac
    23701 cacaaacatc cacgatggga tggaaggcac cagagcttca aaatagattt tcgccagcag
    23761 aatgttgatc atcggcgcga acagatagtc gagcagagaa gaccaaccca ccataaagcc
    23821 gacagtcggg ctaatggatt tctgggcgta agtatatgca gagccagcag aaggatagcg
    23881 gcgaaccagc ttcccgtagc tcagagccgt aaacaggatc gcaatcaatg cgaacgcata
    23941 ggctgtcggc acatgaccat ccgtgaggcc tgaaacgata ccaaaagtat caaacagcgt
    24001 catcggctgc atataggcaa gacccatcat tacaaccgga actaacgtaa gcgttttacg
    24061 caattccacg cgagaggtgt ttggagtaac gttatgcgac atggtcattc tcctttacgg
    24121 tgataaccgt cgcgtaagca aaaaattgcc ccattttttt ggattcctca gcgacaacaa
    24181 ctgtcgattt ttagtaaata tctatccggt acgaagcccg gcctcttggt atgaatgatt
    24241 ggtttgaagc aaaaaataac cgacgctgat gaaacgtcgg tttttagtca ttttttgaca
    24301 gcggcgcatt gtgcctaaat gggggggaaa tgacaagaga atgagaagct tgtcataata
    24361 atttttcttt aaatggctga ttttccgtca tcagattttg cataaacacc ggcgatcgca
    24421 ctatttgcta aaatttcatc ccgccaccag ccgctggcaa gccctgcggt ctgttcgttc
    24481 cagccaatcc agacgggttc aaagagagtt tgggcgacca ctttcttctc gattaacgcg
    24541 ccactatcga gaaaacgttg caccagataa cggggtaaat agccgcagcc gaggccgcta
    24601 atttgcagct ccagcttggt tttaaaatcg aaaacggtaa tggcttcttg ttcgtcgaga
    24661 agttgtgagg ccgtagaagc gcctgcctgc gcagtgtccc ccaccacaat cgcccgatat
    24721 cgcttaatga tacgacggtt taacggctct tcttcctggg ctaacggatg atgaggcgcg
    24781 accgcaaaga cttgttccag atcgcccaac cgcgagaaac caaactcact ggaagatggt
    24841 ggctcatgca tcgcgccaac aataatatcc gctcgcccct gagtcagggc atcccaggaa
    24901 ccaccgagta cgccattgat aaacttcagg cgcgtcacgc tatgatgttg ataaaaagct
    24961 tcaatcagtg gcgcaagcag agaaaaagga aacgtgtcgt ccacgccaat caccagctcg
    25021 ttttcccagc cttcatgcag tttgatcgcc tgcttttcca gttctcgcac ggtatgcaga
    25081 acttcccgcc ccttctctaa taacattttt ccggtacggg tgaatttagc gcggtgaccg
    25141 ctacgatcaa gtaattgaat attaaggtcg ctttccagct tgtgaacggt gtaactcaga
    25201 gcggacggcg tcttgtagag tttagccgac gccgccgcaa aactgccttc tttttctaag
    25261 gcatcgagga tcatcaacac atccagcagt tgtttcatac acgccccctt gcagtgcatg
    25321 cggcgtcctg ctggcgtgat cgtttactcc agcggcatta ccagcggatc gggatactgg
    25381 tattcaaatc ccagttcatt acaaatccga ctgccatcaa taatcttacc tttgccgctg
    25441 tccagactat ttctgaactg cggaggttcc agccccagta aacgggccat ctgcggatag
    25501 aaaacattac gcgcagggtg agctggcgca catatattat agatgtgtcc gcctttaggt
    25561 gcctgtaaca acagagtgat agcgccaata acatcttcta aatggactaa attaacacca
    25621 tgttcaccat caggcgcggt ttttccggca aagaagcgtc cgggatgacg tcccggtccg
    25681 accagccccg caagacgcag aatatcgacc gaagtaccgg gtaaattgtg cagccagtct
    25741 tcgagttctt ctaatactcg tccactgttg gtcaccggat tacgcggggt ggtttctttc
    25801 accgtgcctt gcgcgtcgcc atagacagac gttgagctgg taaaaataat gcgaggaata
    25861 cgatgggcca gcgcgctatc cactaactct tgtaccgctt gtaaatagaa ctcatcgccg
    25921 gggccgctac gacgtgccgg aagcgtaatg accagcgcat cggcatccat cagggcatcc
    25981 agatcgtcag aatcgcaaac taactcaggc tccatgcgaa gcagatagct atcaatgcca
    26041 ctcattcggg ccgcttcgac accatcttgt gtggttttac tcccggtgac ttgccagcct
    26101 cgcgctgaaa gtgacatcgc cagcggcatg cctaaccacc ctaacccgac aattgcgacc
    26161 tttttcatcc gttatctcct gatacacctt agatctataa ggctacgcta gcgtatcaaa
    26221 actgacaatt cattctatga atgaatctgt tcaataatga taacgacatg ctgcaatgag
    26281 cagtgaatcg tcggtaaccg cgtataccag acggtgctcc tctgtaatgc gtcgggacca
    26341 gaaacctgac aaattatgtt tcaggggttc tggcttcccc ttaccttcaa atggcgttct
    26401 gcgggtatct ttgataagtt cattgatctt tttaacaatt cgcttatctg tttcctgcca
    26461 gtacagataa tcatcccatg attcctcaga ccagattagt ttcactcaat aatgtccttt
    26521 tctgttcctt tgcctgattt caggctatcg attgagtcca tcaatctccg ggcgttagcg
    26581 ggggagcgca gtagataagc cgtctcttcc agcgagttgt attcttcgag tgacatcaga
    26641 acacaagcct ctccattctg acgagtaata aggatcgggg catgatcttc aacggctttc
    26701 atcattgttg ccgacaaatt ctgacgcgct tcgctgtagc taattgtacg catgtcaatc
    26761 tcctcttttg tacagtttat tgtacaatga tgggcgttaa ttaactattt attaattagt
    26821 ttgtagatca aggtattgtc agtgagacga aaatccaggc ttcgctattt ttggtgccgt
    26881 cagcttagag gacagtcctc ttagccccct cctttccccg ctcattcatt aaacaaatcc
    26941 attgccataa aatatataaa aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa
    27001 agacatcagt tgaataaaca ttcacagaga cttttatgac acgcgttcaa tttaaacacc
    27061 accatcatca ccatcatcct gactagtctt tcaggcgatg tgtgctggaa gacattcaga
    27121 tcttccagtg gtgcatgaac gcatgagaaa gcccccggaa gatcaccttc cgggggcttt
    27181 tttattgcgc ggttgataac ggttcagaca ggtttaaaga ggaataacaa aatgacagac
    27241 aacactcgtt tacgcatagc tatgcagaaa tccggccgtt taagtgatga ctcacgcgaa
    27301 ttactggcgc gctgtggcat taaaattaat cttcacaccc agcgcctgat cgcgatggca
    27361 gaaaacatgc cgattgatat tctgcgcgtg cgtgacgacg acattcccgg tctggtaatg
    27421 gatggcgtgg tagaccttgg gattatcggc gaaaacgtgc tggaagaaga gctgcttaac
    27481 cgccgcgccc agggtgaaga tccgcgctac tttaccttgc gtcgtctgga tttcggcggc
    27541 tgccgccttt cgctggcaac gccggttgat gaagcctggg acggcccgct ctccttaaac
    27601 ggtaaacgta tcgccacctc ttatcctcac ctgctcaagc gttatctcga ccagaaaggc
    27661 atctctttta aatcctgctt actgaacggt tctgttgaag tcgccccgcg tgccggactg
    27721 gcggatgcga tttgcgatct ggtttccacc ggtgccacgc tggaagcaaa cggcctgcgc
    27781 gaagtcgaag ttatctaccg ctcgaaagcc tgccttatcc agcgcgatgg tgaaatggaa
    27841 gaatccaaac agcagctgat cgacaaactg ctgacccgta ttcagggtgt gatccaggcg
    27901 cgcgaatcaa aatacatcat gatgcacgca ccgaccgaac gtctggatga agtcatcgcc
    27961 ctgctgccag gtgccgaacg cccaaccatt ctgccgctgg cgggtgacca acaacgcgta
    28021 gcgatgcaca tggtcagtag cgaaaccctg ttctgggaaa cgatggaaaa actgaaagcg
    28081 ctgggtgcca gttcaattct ggtcctgccg attgagaaga tgatggagtg atcgccatga
    28141 gctttaacac aatcattgac tggaatagct gtactgcgga gcaacaacgc cagctgttaa
    28201 tgcgcccggc gatctccgct tctgaaagca ttacccgcac tgttaacgat attctcgata
    28261 acgtgaaagc acgcggtgat gacgccctgc gggaatacag cgcgaagttt gataaaacca
    28321 cggttaacgc gctgaaggtg tctgctgagg agattgccgc cgctagcgaa cgcctgagcg
    28381 acgagctaaa acaggcgatg gcggtggcag taaaaaatat tgaaaccttc cacactgcgc
    28441 aaaaactgcc gccggtagat gtagaaacgc agccaggcgt gcgttgccag caggtcacgc
    28501 gcccggtcgc ttcagttggg ttgtatattc ctggcggctc cgccccgctc ttctcaacgg
    28561 tattaatgct ggcgactccg gcgcgtattg cgggctgtaa aaaagtggtg ctgtgctcac
    28621 cgccgccgat tgccgatgag atcctttatg cggcgcagct gtgcggtgtg aaggacgtgt
    28681 ttaacgtcgg cggcgcacag gccattgccg cgctggcgtt tggtacggaa tctgtgccga
    28741 aagtggacaa aatcttcggg ccgggtaacg cctttgtcac cgaagcgaaa cgtcaggtga
    28801 gccagcgtct ggacggtgcg gctatcgata tgcccgcagg cccgtcggaa gtgttggtca
    28861 ttgccgacag cggcgcaacg ccggatttcg tggcttctga tttgctttct caggctgaac
    28921 acggcccgga ctcacaggtg attttactga cgcctgacgc tgatatggcg catcaagttg
    28981 ccgaagccgt cgaacgccag ttagcagaac tgccgcgtgc cgaaaccgcc cgccaggcac
    29041 tgaacgccag ccgcctgatc gtgactaaag atttagcgca gtgcgtagag atctccaacc
    29101 agtacggccc ggagcacctg atcatccaga cccgcaacgc ccgcgatctg gtcgatggca
    29161 tcaccagcgc cggttcggta tttcttggtg actggtcacc ggaatcggca ggtgattacg
    29221 cctccggcac caaccacgtt ctgccgactt acggttacac cgccacctgt tccagcctcg
    29281 ggctggcaga tttccagaag cgtatgaccg tgcaggaact gtcgaaagaa ggtttctccg
    29341 cgctggcttc gaccattgaa acattggccg ccgccgagcg cctgaccgcc cataaaaatg
    29401 ccgttacttt gcgtgttaac gcccttaagg agcaagcatg agcaccgtga ctattaccga
    29461 tttagcgcgt gaaaacgtcc gcaacctgac gccgtatcag tcggcgcgtc gtctgggcgg
    29521 taatggcgac gtctggctga acgccaacga ataccccact gccgtggagt ttcagcttac
    29581 tcagcaaacg ctcaaccgct acccggaatg tcagccgaaa gcggtgattg aaaattacgc
    29641 gcaatatgca ggcgtaaaac cggaacaggt gctggtcagt cgtggcgcgg acgaaggtat
    29701 tgaactgctg attcgcgctt tttgcgaatc gggtaaagac gccatcctct actgcccgcc
    29761 aacgtacggc atgtacagcg tcagcgccga aacgattggc gtcgagtgcc gcacagtgcc
    29821 gacgcaggac aactggcaac tggacttaca gggcatttcc gacaagctgg acggcgtaaa
    29881 agtggtctat gtttgcagcc ccaacaaccc gacagggcaa ctgatcaatc cgcaggattt
    29941 tcgcactctg ctggagctaa cccgcggaaa agccattgtg gttgccgatg aagcctatat
    30001 cgagttttgc ccgcaggcat cgctggctgg ctggctgacg gaatatccgc acctggctat
    30061 tttgcgcaca ctgtcgaaag cttttgctct ggcgggcctt cgttgcggat ttacgctggc
    30121 aaacgaagaa gtcatcaacc tgctgatgaa agtgatcgcc ccctacccgc tctcgacgcc
    30181 ggttgccgac attgcggccc aggcgttaag cccgcaggga atcgtcgcta tgcgcgaacg
    30241 agtgacgcaa attattgcag aacgcgaata cctgattgcc gcactgaaag agatcccctg
    30301 cgtagagcag gttttcgact ccgcaaccaa ctacattctg gcgcgcttta aagcctccag
    30361 cgcagtgttt aaatctttgt gggatcaggg cattatctta cgtgatcaga ataaacaacc
    30421 ctctttaagc ggctgcctgc gaattaccgt cggaacccgt gaagaaagcc agcgcgtcat
    30481 tgacgcctta cgtgcggagc aagtttgatg agtcagaagt atctttttat cgatcgcgat
    30541 ggaaccctga ttagcgaacc gccgagtgat tttcaggtgg accgttttga caaactcgcc
    30601 tttgaaccag gcgtgatccc ggaactgctg aagctgcaaa aagcgggcta caaactggta
    30661 atgatcacca atcaggatgg tctgggaaca caaagtttcc cgcaggcgga tttcgatggc
    30721 ccgcacaacc tgatgatgca gatcttcacc tcgcaaggcg tacagtttga tgaagtgctg
    30781 atttgtccgc acctgcccgc cgatgagtgc gactgccgta agccgaaagt aaaactggtg
    30841 gaacgttatc tggctgagca agcgatggat cgcgctaaca gttatgtgat tggcgatcgc
    30901 gcgaccgaca ttcaactggc ggaaaacatg ggcattaatg gtttacgcta cgaccgcgaa
    30961 atcctgaact ggccaatgat tggcgagcag ctcactaaac gagaccgtta cgctcacgta
    31021 gtgcgcaaca ccaaagagac acagattgac gttcaggtgt ggctggatcg cgaaggtggc
    31081 agcaagatta acaccggtgt tggcttcttt gatcacatgc tggatcagat cgccacccac
    31141 ggtggtttcc gtatggaaat caacgtcaaa ggcgacctct atatcgacga tcaccacacc
    31201 gtcgaagata ccggcctggc gctgggcgaa gcgttaaaaa tggctcttgg cgacaaacgc
    31261 ggtatttgcc gctttggttt tgtgctaccg atggacgaat gccttgcccg ctgcgcgctg
    31321 gatatctctg gtcgcccgca cctggaatac aaagccgagt ttacctacca gcgcgtgggc
    31381 gatctcagca ccgagatgat cgagcacttc ttccgttcgc tctcatacac catgggcgtg
    31441 acgctacacc tgaaaaccaa aggtaaaaac gatcaccacc gtgtagagag cctgttcaaa
    31501 gcctttggtc gcaccctgcg ccaagccatc cgcgtggaag gcgacaccct gccctcgtcg
    31561 aaaggagtgc tgtaatgaac gtggtgatcc ttgataccgg ctgcgccaat ctgaactcgg
    31621 tgaagtcagc cattgcgcgc cacggttatg aaccaaaagt cagccgtgac ccggacgtgg
    31681 tgttgctggc cgataaacta tttttacccg gcgttggcac tgcgcaagca gcgatggatc
    31741 aggtgcgtga gcgcgagctg tttgatctca tcaaagcctg tacccaaccg gtactgggca
    31801 tctgcttagg gatgcaactg ctgggtcggc gcagcgaaga gagcaacggc gtcgacttgc
    31861 tgggcatcat tgacgaagac gtgccgaaaa tgaccgactt tggtctgccg ctgccgcata
    31921 tgggctggaa tcgcgtttac ccgcaggcag gcaaccgcct gtttcagggg attgaagacg
    31981 gcgcgtactt ttactttgtt cacagttacg caatgccggt caatccgtgg accattgccc
    32041 aatgtaatta cggcgaaccg ttcaccgcgg cggtacaaaa agataacttc tacggcgtgc
    32101 agttccaccc ggagcgttct ggtgccgctg gcgctaagtt gctgaaaaac ttcctggaga
    32161 tgtgatgatt attccggcat tagatttaat cgacggcacc gtggtgcgtc tccatcaggg
    32221 cgattacggc aaacaacgcg attacggtaa cgacccgctg ccgcgcttac aggattacgc
    32281 cgcgcagggt gccgaagtgc tgcatctggt ggatctgacc ggggcaaaag atccggcaaa
    32341 acgtcaaatc ccgctgatta aaaccctggt cgcgggcgtt aacgttccgg tgcaggttgg
    32401 cggcggtgtg cgtagcgaag aagacgtggc ggcgttactg gaagcgggcg ttgcgcgcgt
    32461 ggtggttggc tccaccgcgg tgaaatcacc agaaagggtg aaaggctggt ttgaacgttt
    32521 cggtgccgat gccttagtgc tggcgctgga tgtccgtatt gacgagcaag gcaacaagca
    32581 ggtggcggtc agcggctggc aagagaactc gggcgtttca ctggaacaac tggtggaaac
    32641 ctatctgcct gtcggcctga aacatgtgct gtgtaccgat atctcgcgcg acggcacgct
    32701 ggcaggctct aacgtctctt tatatgaaga agtgtgcgcc agatacccgc aggtggcatt
    32761 tcagtcctcc ggcggtattg gcgacattaa cgatgttgct gccctgcgag gcactggcgt
    32821 gcgcggcgta atagttggtc gtgcattact ggaaggtaaa ttcaccgtga aggaggccat
    32881 cgcatgctgg caaaacgcat aatcccatgt ctcgacgttc gtgatggtca ggtggtgaaa
    32941 ggcgtacagt ttcgcaacca tgaaattatt ggcgatattg taccgctggc aaaacgctac
    33001 gccgaagaag gtgcagacga actggtgttc tacgatatca ccgcttccag cgatggccgt
    33061 gtggtagata aaagctgggt atctcgcgtg gcggaagtga tcgacattcc gttctgcgtg
    33121 gcgggtggga ttaagtctct ggaagatgcc gcgaaaattc tttcctttgg cgcggataaa
    33181 atttccatca actcccctgc gctggcagac ccaaccttaa ttacccgcct ggcggatcgc
    33241 tttggcgtgc aatgcattgt ggtcggcatt gatacctggt acgacggcga aaccggtaaa
    33301 tatcatgtga atcaatatac cggcgatgaa agccgcaccc gcgtcactca gtgggaaaca
    33361 ctcgactggg tacaggaagt gcaaaaacgt ggagccggag aaatcgtcct caacatgatg
    33421 aatcaggacg gcgtgcgtaa cggttacgat ctcgaacaac tgaaaaaagt gcgtgaagtt
    33481 tgccacgtcc cgctaattgc ctccggtggc gcgggcacca tggaacactt cctcgaagcc
    33541 ttccgcgatg ccgacgttga cggcgcgctg gctgcttccg tattccacaa acaaataatc
    33601 aatattggtg aattaaaagc gtacctggca acacagggcg tggagatcag gatatgttaa
    33661 cagaacaaca acgccgcgaa ctggactggg aaaaaaccga cggactaatg ccggtaattg
    33721 tgcaacacgc ggtatccggc gaagtgttaa tgctgggcta tatgaacccg gaagccttag
    33781 acaaaaccat cgaaagcggc aaagtcacct tcttctcgcg cactaaacag cgactgtgga
    33841 ccaaaggcga aacgtcgggc aatttcctca acgtagtaaa tattgcgccg gactgcgaca
    33901 acgacacgct actggtactg gcgaatccta tcggaccaac ctgccacaaa ggcaccagca
    33961 gctgcttcgg cgacaccgct caccaatggc tgttcctgta tcaactggaa caactgctcg
    34021 ccgaacgcaa atctgccgac ccggaaacct cctataccgc caaactgtat gccagcggca
    34081 ccaaacgcat tgcgcagaaa gtgggcgaag aaggcgtaga aaccgcactg gcagcgacgg
    34141 tacatgaccg ctttgagctg accaacgagg catcggactt gatgtatcac ctgctggtgc
    34201 tgttgcagga tcaggatctg gatttaacga cggtaattga gaacctgcgt aaacggcatc
    34261 agtgagttgc agggtaagcg gatgcgatgt tgttgtcgca tccggcaaaa aaaaagggca
    34321 aggtgtcacc accctgcccg ttttctttaa agctgcaaag attacttcgc gttataatta
    34381 cgcagagcgt tacgccccag cacaatcccc gcgccaacca tgccgcccag cagcaccgcc
    34441 agaatcaagg taattgcctt tttcgggcta tcgcgacgaa taggtaacgt cggtttcatc
    34501 acataacggt aagcatgaat atcaagatca tcaaccttta agttttcaat atccaacagg
    34561 ttttgacgtg tctgatagta gtttggtgag aacaccaacg gacgggtcgc ctcatgctta
    34621 atcatcgact ccagcgcttc gctccccaaa aggaacaacg tatcttgtgt cacatcttca
    34681 gtctgctgaa cctgtggctt tgccacctgc gcctgattcg catactgcaa cgcttcctga
    34741 atctgacgga tacgcagatc tttctgctcc tgcgcgacta cttcctgcgt tcttaaagag
    34801 tcctgcaagt ttttccgtcc cagagcaatg ttgtctttga gatccttttc tagctcttga
    34861 ttcactttat catcaacttg ctgaatgtat tgggccaact tcatttgtgc tccctctgca
    34921 gtttgcccaa cataagaaac agtcaatggt aattgctggt tcttaacaga aggttcgatg
    34981 gtaagttttt ctggttcttc ctgattatcc agcgtttctg ctaatgcaga gaaggcagaa
    35041 ctgaagcgac caattaacgt ctcctgtaaa tccgatactt tcggtgcagc ctgaccatag
    35101 ataacattca tggcattgtt atagccagca atttgcccca cgtcgggctg agtgataatt
    35161 gctgttgacg tccatttctc cttcgctact gccaaatatc caatagccag ggcaatagcc
    35221 acaatgacgg aaatgatgat tgtcatcttg ccacgccaca actgcgctag taaatcaatc
    35281 aaatcaatct gttccgggtc atggttttgc ccagaaacat tattattttc tactctcatc
    35341 attaccctaa ctgacggaaa tttccaagtc cgcatagttt agcgataatc ctagtgaatg
    35401 ctataggata agtgataaaa ataatgaatt attggggagg taacaaaaga caatcagggc
    35461 gtaaatagcc ctgataacaa gatgttagtc gctgccaaag agatcgcggg tgtatacctt
    35521 atctgccaca tctttaagct cttctgccat tcggttagag ataatgacgt cggcttgttg
    35581 tttgaagatg gcgagatcac gttccagccg agagttgaag aatgagtctt ctttcatcac
    35641 tggctcgtag atgatcactt caacgccttt cgccttgata cgcttcataa tcccctgaat
    35701 agaagacgca cggaagttat ctgaaccgct cttcataatc agacgataaa tacccaccac
    35761 ttgcggtttg cgtgacaaaa tggcatcggc aataaaatcc ttacgcgtgc ggtttgcatc
    35821 gacaattgcc gagatcaggt tattcggcac agactgatag ttcgccagta actgcttggt
    35881 atctttcggc aagcaataac caccataacc aaacgacggg ttgttgtaat ggttgccaat
    35941 acgcggatcg agacaaacgc cttcgataat ctggcgagta ttcagaccta aactttctgc
    36001 gtagctatcc agttcgttaa agtatgccac gcgcatcgct aggtaggtgt ttgcaaaaag
    36061 tttaatcgct tctgcttcgg tcgaatcggt gaacagggtc gggatatttt gcttaatcgc
    36121 gccttcctgt aacaacgcag caaaacgttc tgcgcgttct gaacgctcac cgatgacaat
    36181 acgtgaagga tggagattat cgtaaagggc tttaccttca cggagaaatt ccggagagaa
    36241 aataatattt tcagtacgat atttcttatg catcgcttcg gtaaaaccaa cgggaaccgt
    36301 tgatttgatg accataaccg cataaggatt tatctcaact acgtctttaa ttactgattc
    36361 tacactggat gtattgaaat aattagtttt aggatcatag tcggttggag tggcgatgat
    36421 gacataatca gcatcccggt aagcttcatt tttatctaat gtggcattaa agtgtatttt
    36481 atctgattgc aaaaactgct gaatttcctt atcaacaata ggagatatcc gatcattcag
    36541 catagcaacg cgtgacggta aaatatctaa tgccacaacc tcatgatttt gtgcgattag
    36601 aagcccgttt gacaagccta catagccagt accggaaatg gtgattttca tttcatgctc
    36661 tcagaattaa cttaactgtg aatcatgatg tttttagcat cctgataaga gctaaatgtt
    36721 ttaacgctac gttattagct ggtcaacgct acgaaaaaca attcagat
//
LOCUS       DAZSBF010000011        36750 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZSBF010000011 DAZSBF010000000
VERSION     DAZSBF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36750
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.011"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHXWD_000412"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHXWD_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR7619435.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHXWD_000413"
     CDS             complement(280..873)
                     /locus_tag="ACHXWD_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR7619436.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHXWD_000414"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHXWD_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7619437.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHXWD_000415"
     CDS             1788..2183
                     /locus_tag="ACHXWD_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR7619438.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHXWD_000416"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHXWD_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR7619439.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHXWD_000417"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHXWD_000417"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR7619440.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHXWD_000418"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHXWD_000418"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR7619441.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHXWD_000419"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHXWD_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR7619442.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHXWD_000420"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHXWD_000420"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR7619443.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHXWD_000421"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHXWD_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR7619444.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHXWD_000422"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHXWD_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR7619445.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHXWD_000423"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHXWD_000423"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR7619446.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHXWD_000424"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHXWD_000424"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR7619447.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHXWD_000425"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHXWD_000425"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR7619448.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHXWD_000426"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHXWD_000426"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR7619449.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHXWD_000427"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHXWD_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR7619450.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHXWD_000428"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHXWD_000428"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR7619451.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHXWD_000429"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHXWD_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR7619452.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHXWD_000430"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHXWD_000430"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR7619453.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHXWD_000431"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHXWD_000431"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR7619454.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHXWD_000432"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHXWD_000432"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR7619455.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHXWD_000433"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHXWD_000433"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR7619456.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHXWD_000434"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHXWD_000434"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR7619457.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHXWD_000435"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHXWD_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR7619458.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHXWD_000436"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHXWD_000436"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR7619459.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHXWD_000437"
     CDS             complement(26207..26548)
                     /locus_tag="ACHXWD_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR7619460.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHXWD_000438"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHXWD_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR7619461.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHXWD_000439"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHXWD_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR7619462.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHXWD_000440"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHXWD_000440"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR7619463.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHXWD_000441"
     CDS             33227..34570
                     /locus_tag="ACHXWD_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR7619464.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHXWD_000442"
     CDS             complement(34650..34856)
                     /locus_tag="ACHXWD_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR7619465.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHXWD_000443"
     CDS             35181..35735
                     /locus_tag="ACHXWD_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR7619466.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHXWD_000444"
     CDS             complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHXWD_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR7619467.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
BASE COUNT         8902 a         9847 c         9135 g         8866 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tcttgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccatt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt
//
LOCUS       DAZSBF010000012        36067 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZSBF010000012 DAZSBF010000000
VERSION     DAZSBF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.012"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            109..672
                     /gene="srlA"
                     /locus_tag="ACHXWD_000445"
                     /gene_synonym="gutA"
     CDS             109..672
                     /gene="srlA"
                     /locus_tag="ACHXWD_000445"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR7619468.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFLHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            669..1628
                     /gene="srlE"
                     /locus_tag="ACHXWD_000446"
                     /gene_synonym="gutE"
     CDS             669..1628
                     /gene="srlE"
                     /locus_tag="ACHXWD_000446"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR7619469.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            1639..2010
                     /gene="srlB"
                     /locus_tag="ACHXWD_000447"
                     /gene_synonym="gutB"
     CDS             1639..2010
                     /gene="srlB"
                     /locus_tag="ACHXWD_000447"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR7619470.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            2014..2782
                     /gene="srlD"
                     /locus_tag="ACHXWD_000448"
                     /pseudo
     CDS             2014..2782
                     /gene="srlD"
                     /locus_tag="ACHXWD_000448"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            2888..3247
                     /gene="gutM"
                     /locus_tag="ACHXWD_000449"
     CDS             2888..3247
                     /gene="gutM"
                     /locus_tag="ACHXWD_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR7619471.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3314..4087
                     /gene="srlR"
                     /locus_tag="ACHXWD_000450"
                     /gene_synonym="gutR"
     CDS             3314..4087
                     /gene="srlR"
                     /locus_tag="ACHXWD_000450"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR7619472.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4080..5045
                     /gene="gutQ"
                     /locus_tag="ACHXWD_000451"
     CDS             4080..5045
                     /gene="gutQ"
                     /locus_tag="ACHXWD_000451"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR7619473.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5042..6556)
                     /gene="norR"
                     /locus_tag="ACHXWD_000452"
     CDS             complement(5042..6556)
                     /gene="norR"
                     /locus_tag="ACHXWD_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR7619474.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            6743..8182
                     /gene="norV"
                     /locus_tag="ACHXWD_000453"
     CDS             6743..8182
                     /gene="norV"
                     /locus_tag="ACHXWD_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR7619475.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8179..9312
                     /gene="norW"
                     /locus_tag="ACHXWD_000454"
     CDS             8179..9312
                     /gene="norW"
                     /locus_tag="ACHXWD_000454"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR7619476.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(9440..11692)
                     /gene="hypF"
                     /locus_tag="ACHXWD_000455"
     CDS             complement(9440..11692)
                     /gene="hypF"
                     /locus_tag="ACHXWD_000455"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR7619477.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(11845..12372)
                     /gene="hydN"
                     /locus_tag="ACHXWD_000456"
     CDS             complement(11845..12372)
                     /gene="hydN"
                     /locus_tag="ACHXWD_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR7619478.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(12520..13530)
                     /gene="ascG"
                     /locus_tag="ACHXWD_000457"
     CDS             complement(12520..13530)
                     /gene="ascG"
                     /locus_tag="ACHXWD_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR7619479.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            13790..15247
                     /gene="ascF"
                     /locus_tag="ACHXWD_000458"
     CDS             13790..15247
                     /gene="ascF"
                     /locus_tag="ACHXWD_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR7619480.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            15256..16680
                     /gene="ascB"
                     /locus_tag="ACHXWD_000459"
     CDS             15256..16680
                     /gene="ascB"
                     /locus_tag="ACHXWD_000459"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR7619481.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(16794..17213)
                     /locus_tag="ACHXWD_000460"
     CDS             complement(16794..17213)
                     /locus_tag="ACHXWD_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7619482.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(17210..17521)
                     /locus_tag="ACHXWD_000461"
     CDS             complement(17210..17521)
                     /locus_tag="ACHXWD_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR7619483.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(17683..18456)
                     /locus_tag="ACHXWD_000462"
     CDS             complement(17683..18456)
                     /locus_tag="ACHXWD_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619484.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(18481..18951)
                     /gene="hycI"
                     /locus_tag="ACHXWD_000463"
     CDS             complement(18481..18951)
                     /gene="hycI"
                     /locus_tag="ACHXWD_000463"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR7619485.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(18944..19354)
                     /gene="hycH"
                     /locus_tag="ACHXWD_000464"
     CDS             complement(18944..19354)
                     /gene="hycH"
                     /locus_tag="ACHXWD_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR7619486.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(19351..20118)
                     /gene="hycG"
                     /locus_tag="ACHXWD_000465"
     CDS             complement(19351..20118)
                     /gene="hycG"
                     /locus_tag="ACHXWD_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR7619487.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(20118..20660)
                     /gene="hycF"
                     /locus_tag="ACHXWD_000466"
     CDS             complement(20118..20660)
                     /gene="hycF"
                     /locus_tag="ACHXWD_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR7619488.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(20670..22367)
                     /gene="hycE"
                     /locus_tag="ACHXWD_000467"
     CDS             complement(20670..22367)
                     /gene="hycE"
                     /locus_tag="ACHXWD_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR7619489.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(22385..23308)
                     /gene="hycD"
                     /locus_tag="ACHXWD_000468"
     CDS             complement(22385..23308)
                     /gene="hycD"
                     /locus_tag="ACHXWD_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR7619490.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(23311..25137)
                     /gene="hycC"
                     /locus_tag="ACHXWD_000469"
     CDS             complement(23311..25137)
                     /gene="hycC"
                     /locus_tag="ACHXWD_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR7619491.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCDYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25134..25745)
                     /gene="hycB"
                     /locus_tag="ACHXWD_000470"
     CDS             complement(25134..25745)
                     /gene="hycB"
                     /locus_tag="ACHXWD_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR7619492.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(25870..26331)
                     /gene="hycA"
                     /locus_tag="ACHXWD_000471"
     CDS             complement(25870..26331)
                     /gene="hycA"
                     /locus_tag="ACHXWD_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR7619493.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26543..26893
                     /gene="hypA"
                     /locus_tag="ACHXWD_000472"
     CDS             26543..26893
                     /gene="hypA"
                     /locus_tag="ACHXWD_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR7619494.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            26897..27769
                     /gene="hypB"
                     /locus_tag="ACHXWD_000473"
     CDS             26897..27769
                     /gene="hypB"
                     /locus_tag="ACHXWD_000473"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR7619495.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            27760..28032
                     /gene="hypC"
                     /locus_tag="ACHXWD_000474"
     CDS             27760..28032
                     /gene="hypC"
                     /locus_tag="ACHXWD_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR7619496.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28032..29153
                     /gene="hypD"
                     /locus_tag="ACHXWD_000475"
     CDS             28032..29153
                     /gene="hypD"
                     /locus_tag="ACHXWD_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR7619497.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            29150..30160
                     /gene="hypE"
                     /locus_tag="ACHXWD_000476"
     CDS             29150..30160
                     /gene="hypE"
                     /locus_tag="ACHXWD_000476"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR7619498.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30234..32311
                     /gene="flhA"
                     /locus_tag="ACHXWD_000477"
                     /pseudo
     CDS             30234..32311
                     /gene="flhA"
                     /locus_tag="ACHXWD_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(32348..32701)
                     /locus_tag="ACHXWD_000478"
     CDS             complement(32348..32701)
                     /locus_tag="ACHXWD_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR7619499.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(32778..32912)
                     /locus_tag="ACHXWD_000479"
     CDS             complement(32778..32912)
                     /locus_tag="ACHXWD_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619500.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            32988..35549
                     /gene="mutS"
                     /locus_tag="ACHXWD_000480"
     CDS             32988..35549
                     /gene="mutS"
                     /locus_tag="ACHXWD_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR7619501.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            35655..36067
                     /locus_tag="ACHXWD_000481"
                     /pseudo
     CDS             35655..36067
                     /locus_tag="ACHXWD_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         8563 a         9850 c         9543 g         8111 t
ORIGIN      
        1 aatatttatc tttcattttg cgatcaaaat aacactttca aatctttcaa tctgattaga
       61 ttaggttgcc gtttggtaat aaaacaataa atcctgaagg agagaacaat gatagaaacc
      121 attactcatg gtgcagagtg gtttatcggg ctgttccaaa agggcggaga ggtgtttacc
      181 gggatggtga ccggcattct tccgctgttg attagcctgc tggttatcat gaacgcactg
      241 attaatttta tcggtcagca tcgtattgaa cgttttgctc aacgttgcgc cggtaaccct
      301 gtttcccgtt acctgctgct accgtgcatt ggcacgtttg tcttttgcaa tccgatgacc
      361 ttaagcctgg gtcgctttat gccggaaaag tacaaaccca gctactacgc ggcagcctct
      421 tatagctgcc actcaatgaa tggcctcttt ctccatatca accctggcga actgtttgtt
      481 tatcttggca ttgccagcgg tctgacaacg ctgaacctgc cacttggccc actggcggtg
      541 agctatctgc tggttggtct ggtcaccaac ttctttcgcg gctgggtgac cgatctgacc
      601 accgccattt ttgagaaaaa gatgggcatt caacttgaac aaaaagttca cctggcagga
      661 gcaacatcat gacgcgtatt cggatcgaaa aaggaacggg tggctggggc ggcccgcttg
      721 agctggaagc cacgccgggc aaaaaaatcg tctatatcac cgccggtacc cggcctgcga
      781 ttgttgacaa actggcacaa cttactggct ggcaggctat tgacggattt aaagaaggtg
      841 aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca ttacgctgcg
      901 gcatctatcc gaaacgacgt attcccacca ttaatatcca ctcgacgggc aagtccagtc
      961 cgctggcgca gtacattgtg gaagatattt atgtctctgg cgtaaaagaa gaaaacatca
     1021 ctgtagtggg tgatgcgaca ccacaaccct cttccgtggg ccgtgactat gacaccagca
     1081 agaaaatcac cgaacaaagc gatggtttac tggcgaaggt gggaatgggt atgggttctg
     1141 ccgttgccgt gttgtttcaa tctggtcgtg acaccatcga cactgtatta aaaaccattc
     1201 tgccgtttat ggcgttcgtc tcggcgctca ttggcatcat tatggcttct ggccttggtg
     1261 actggattgc ccacggtctt gctccgctgg cgagccatcc actaggtctg gtcatgctgg
     1321 cgctcatctg ctccttcccg ctgctttcac ctttcctcgg cccaggcgca gttatcgcac
     1381 aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg ccgcatctgg
     1441 ctttaccggc actgtttgcc atcaacgcgc aggcggcctg cgacttcatc ccggtcggtt
     1501 tgtcgctggc ggaagcccgt caggacacgg ttcgcgtcgg tgtcccttct gtactggtga
     1561 gccacttttt aaccggcgca ccgactgtac tgatcgcctg gtttgtctcc ggttttatct
     1621 atcaatagag gctgaaacat gaccgttatt tatcagacca ccatcacccg tatcggcgcg
     1681 agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg cgcgccagcg
     1741 gacctcgaag agtattgctt cattcattgc cacggcgagt tgaaaggtgc actccatccc
     1801 ggtttgcaat ttacgctcgg gcagcatcgc tatccggtga ccgctgttgg cagcgtggcg
     1861 gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt aaacgaagcg
     1921 gaatttccgg gcactgtcca tgtggcaggc cctgtacccg acgatatcgc gccgggatcg
     1981 gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc aggttgccgt tgtcatcggt
     2041 ggtgggcaaa ccttaggcgc gttcctgtgc cacggtctgg ctgccgaggg gtatcgcgtc
     2101 gcggttgtcg atattcagag cgacaaagcc gcaaatgtgg cacaagaaat taacgccgaa
     2161 tatggtgaag gaacagcgta tagttttggt gctgacgcca ccagcgagca aagcgtcctg
     2221 gcgctctctc gtggggtaga tgaaatcttt gggcgcgtgg atttgctggt ctacagcgcc
     2281 ggaatagcaa aagcagcctt tatcagcgac ttccagctcg gcgattttga ccgtgaatct
     2341 ggtgggttat ttcctgtgtg cccgtgaatt ttctcgtttg atgatccgcg acgggattca
     2401 gggtcgcatt attcagatca actcgaaatc cggcaaagtg ggcagcaaac acaactctgg
     2461 ctacagcgca gcgaaatttg gtggcgtcgg gctgactcaa tcactggcgc tggatctggc
     2521 ggagtacggc attacggtgc attcactgat gctcggtaac ctgctgaaat cgcctatgtt
     2581 ccagtcactg ttaccccaat acgcgaccaa gctgggtatc aaaccggatc aagtcgagca
     2641 gtattacatc gacaaagtac cgctcaaacg cggctgcgat tatcaggatg tgctgaatat
     2701 gctgctgctc tatgccagtc ctaaggcgtc gtactgcacc ggacagtcga tcaatgtcac
     2761 cggcggtcag gtgatgttgt aatcaacagc ggagatccgt taaggatctc cgtgagacta
     2821 atagaatgcc tgatgcgcta cgctcatcag gcatacagga cttccgccac tacattaagg
     2881 aaaagttatg gtatccgcac tcatcaccgt cgccgttatc gcctggtgtg cgcaactggc
     2941 cttaggcggc tggcaaattt ctcgttttaa ccgtgccttc gacacactat gccagcaagg
     3001 gcgggttggc gtgggccgtt ccagcgggcg ctttaaacca cgggtcgtgg tcgccatcgc
     3061 cctggacgat cagcagcgca tcgtcgacac cttgtttatg aaaggactga ccgtcttcgc
     3121 acgaccgcaa aaaattcccg caattaccgg tatgcatgcg ggtgatttac agcccgatgt
     3181 gatctttccc catgatccac tatcacagaa tgctctatca ttggcgctta aactgaaacg
     3241 tggataattt cgttgtgaat gttacttgct tgcgaagtta tcattttgaa acctaaatca
     3301 ggtaatcacg cccatgaaac ctcgtcagcg tcaggccgcc attctggagt atctgcaaaa
     3361 gcagggtaaa tgctcggttg aagaattggc gcaatacttt gacaccacag gcacaaccat
     3421 tcgcaaagat ctggtcattc tggaacatgc aggaaccgtc attcgtactt atggcggagt
     3481 ggtgttgaat aaagaggaat ccgatccgcc tatcgatcat aaaacactca tcaacaccca
     3541 caagaaagag ctgatagcag aagctgccgt tagttttatt catgatggcg attcgatcat
     3601 tcttgatgct ggcagtaccg ttttgcagat ggttcccctg ctctcgcgct ttaataacat
     3661 cacggtgatg accaacagtc tgcatatcgt caatgcgcta tctgaactgg ataacgaaca
     3721 aactatcctg atgccaggcg gaacgtttcg taaaaaatcg gcctcatttc acggacagct
     3781 ggcagagaat gccttcgaac atttcagttt cgataaattg tttatgggca ccgacggcat
     3841 cgatctcaat gcgggcgtaa ccacctttaa cgaggtttat accgtcagta aggcaatgtg
     3901 caatgccgcg cgcgaagtga ttttgatggc ggactcatca aagtttggcc gtaaaagccc
     3961 caacgtagtt tgcagtcttg aaagcgtcga taagctgatt accgacgcag gtatcgatcc
     4021 ggcgtttcgt caggcgctgg aagagaaagg gatcgatgtg atcataaccg gagagagcaa
     4081 tgagtgaagc actactgaac gcgggacgtc agacgttaat gctggagttg caggaagcaa
     4141 gccgtttacc ggaacgtctg ggcgatgatt ttgttcgcgc cgccaatatc atcctgcact
     4201 gcgaaggcaa agtggtggtt tcgggaattg gcaaatcggg ccacattggt aagaaaatcg
     4261 ccgcaacact tgccagtacc ggcacatcgg ctttttttgt ccatccggca gaagcactgc
     4321 acggcgatct ggggatgatt gaaagccgcg atgtgatgct gtttatctct tactccggtg
     4381 gcgcgaagga actggatctg attattccgc gtctggaaga taaatctatc gcgctgctgg
     4441 cgatgaccgg caaaccgacg tcaccgctgg gcctggcggc aaaagcggtg ctggatatct
     4501 ccgtagaacg cgaagcctgc ccgatgcacc ttgcgccgac ctccagcacc gtcaataccc
     4561 tgatgatggg tgacgcgctg gcgatggcgg tcatgcaggc gcgcggattt aatgaagaag
     4621 attttgcccg ctcccaccca gccggggcac tgggcgctcg cttgctgaat aaagtgcatc
     4681 atctgatgcg ccgtgacgat gccatcccac aggtggcgtt aaccgccagc gtgatggatg
     4741 cgatgctgga actcagccgc accggtctgg ggctggtggc ggtatgtgac gctcaacaac
     4801 aggtacaagg cgtctttacc gacggcgatt tacgtcgctg gctggtaggc ggcggcgcac
     4861 tcaccacgcc agttaatgaa gcgatgacga caggcggcac cacgttacag gcgcaaagtc
     4921 gcgccatcga cgccaaagag atcctgatga agcgcaaaat cactgccgca ccggtggtgg
     4981 atgaaaacgg caaactcacc ggcgcaatta acctgcagga tttctatcag gccgggatta
     5041 tttaatcctt cattcccaga cgtttcgcca gccgatgcag gttggcgacg tcggtttcca
     5101 gcatccgcgc gcaggcagcc cagttatgat gattttgtgc cagtgcctgg cgaatagttt
     5161 cacgctggaa cgcttctgtc gcttcacgca ggttttgctt aacaacgggc accgccgccg
     5221 cttctggcgg cggcaacgtc acctcaggaa aagcaaaatg ttgcgcctca agaatcactt
     5281 catcgccgtt gcgggtggct ctcgccagca ctaccgcccg atgaatagca tgttccagtt
     5341 cgcgcacgtt ccccggaaaa cgatagtgtt gcagtaaatt tcgcgctccg gcacttaata
     5401 ccacgcggga gagccccagc cgcaaacgac actgctcgca gaaatacccc gccagcagaa
     5461 tgacatcatc gccccgctca cgcagcggcg gcaccgaaag tggaaacacg ctcaggcgat
     5521 gaaacaagtc agcgcgaaat cgccctgcca tcacctcttc gcgtaagtcg cggttagtcg
     5581 ccgccagcac gcgcacatcg acccgcaaac tacggtcatc gccaacgcgc tgaatatcgc
     5641 catactgcaa caccctcagc agcttggcct gcaatgccaa cgacaactcg ccgatctcat
     5701 ccagaaacag agtgccgtta tccgccattt caaacttccc gctgcggtta ctgatagcgc
     5761 cagtaaacgc tcctttcaca tgcccgaaca actcactttc cgccacactt tccggcagtg
     5821 cagcacagtt gagatagacc agcggattca ccgcccgtgg cgaggcttca tgaatcgctt
     5881 tcgccaccag ctccttaccg gttcccgtct caccgctgat caggacgttg agatcggacg
     5941 ccgccacaat ctcaatctct tttttcagtt gcgtcatgcc aggggacaag ccaatcatct
     6001 gcgtctgttt caccgcttca aacggcgtgg catcgcctgg catcatattc tggctttcca
     6061 gttgttcaat cagcaacgca ttgcttaacg ctcccgccgc cagcgcagca atcagccgta
     6121 gctcttcgtc gctgaaaaca tcgaactgat cgggctgcat cccgtcgagc gtcagtgcgc
     6181 cgatcaggtt ttgcccggca aacaatggca gaccaacgca ggcgtgaacc ttcagactct
     6241 cctgcccagg aatcaaaccg tcatagggat cgggcaattc gctgtctgcg ggaaagcgca
     6301 ccacatcccc ggcgcgggca atcgcttcca gccgtggatg cccttccagc gcaaagcgtc
     6361 taccgagtac atcctttgcc agaccgtcga tggcaagcgg aataaactgc cgcgaatcgt
     6421 aacgtagcaa cgcagacgca tcgcactcca gcacctgacg tagcgtggtg atcaggcgct
     6481 gaaaacgatc ctgatgacca atcccacgct gcaattcgat ggcgatattc gccagcacat
     6541 caacggaaaa actcatcttt gcctcactgt caatttgact atagatattg tcatatcgac
     6601 catttgattg atagtcattt tgactactca ttaatgagca taattttatt tatagagtaa
     6661 aaacaatcag ataaaaaact ggcacgtaat ctgcaattag caagacatct ttttagaaca
     6721 cgctgaataa attgaggttg ctatgtctat tgtggtgaaa aataacattc attgggttgg
     6781 tcaacgtgac tgggaagtgc gtgattttca cggtacggaa tataaaacgc tacgcggcag
     6841 cagttacaac agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca ccgtcgacca
     6901 taaattcagc cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg cggatatcga
     6961 ttacatcgtg attaaccatg cagaagagga ccacgctggg gcgctgaccg aactgatggc
     7021 gcaaattccc gatacgccga tttactgtac tgccaacgct atcgactcga taaatggtca
     7081 tcatcatcat ccggagtgga attttaatgt ggtgaaaact ggtgacacgc tggatatcgg
     7141 caacggcaaa cagctcattt ttgtcgaaac gccaatgctg cactggccgg acagcatgat
     7201 gacttacctg acaggcgacg cggtgctgtt cagtaacgat gctttcggtc agcactactg
     7261 cgacgagcat ctgttcaacg atgaagtgga tcagacggag cttttcgagc agtgccagcg
     7321 ttactacgcc aatatcctga cgccgttcag ccgcctggta acaccgaaaa ttaccgagat
     7381 cctgggcttt aacttaccag tcgatatgat agcgacctct cacggcgtgg tatggcgcga
     7441 taacccgacg caaattgtcg agctgtacct gaaatgggcg gctgattatc aggaagacag
     7501 aatcaccatt ttctacgaca ccatgtcgaa taacacccgc atgatggctg acgctatcgc
     7561 ccaggggatt gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg tcgcccgaag
     7621 cgataaaaac gaaatcctga ccaatgtctt ccgctcaaaa ggcgtgctgg tcggcacttc
     7681 gacgatgaat aacgtgatga tgccgaaaat cgccggtctg gtggaggaga tgaccggatt
     7741 acgcttccgt aacaaacgcg ccagtgcttt cggctctcac ggctggagcg gcggtgcggt
     7801 ggatcgtctt tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctta gcctgaaagc
     7861 caaatggcga ccagaccagg acgctctgga gttatgccgt gaacatggtc gcgaaatcgc
     7921 ccgtcagtgg gcgctcgcgc cgctgccgca gagcacggtg aatacggtgg ttaaagaaga
     7981 aacctctgcc accacgaagt ccgacctcgg cccacggatg cagtgcagcg tctgccagtg
     8041 gatttacgat ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa cgccgtggag
     8101 tgaagttccg gataacttcc tctgcccgga atgctctctc ggcaaagacg tctttgacga
     8161 actggcatcg gaggcaaaat gagcaacggc attgtgatca ttggttcggg cttcgccgcc
     8221 cgccaactgg tgaaaaatat tcgcaaacag gacgccagta ttccattaac cctgatcgcc
     8281 gccgacagca tggatgagta caacaaaccg gacctcagcc atgttatcag ccaggggcaa
     8341 cgtgccgacg accttacccg ccagacggcg ggtgaatttg ccgggcagtt taatctgcac
     8401 ctgtttccgc acacctgggt gacggatatc gatgccgaag cccatgtggt gaaaagccag
     8461 aataatcagt ggcaatacga caagctggtg ctggcaactg gtgccagtgc ctttgtcccg
     8521 ccagtgcctg ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta tcgcgcctgt
     8581 gaaacgcaac tgcgggatgc ccgacgcgtg ttgattgtcg gcggtggttt gattggtagc
     8641 gaactggcga tggatttttg tcgtgcaggc aaagcggtca cgctaatcga caacgctgcc
     8701 agtattctgg cgtcattaat gccaccagaa gtaagcagcc gcttgcagca tcggttgacg
     8761 gagatgggcg ttcatctgct gttgaaatct cagttgcagg ggctggaaaa aacggattct
     8821 ggcattctgg caacgctgga ccaccagcgt agtatcgaag tggatgcggt aattgccgcc
     8881 accggactgc gcccggaaac agccctggca cgacgcgccg ggctgacgat taatcgcggc
     8941 gtttgcgtcg atagttatct gcaaaccagt aatgacgata tttatgcgct gggcgattgc
     9001 gcggaaatca acggtcaggt actgccattc ctgcagccga ttcaacttag cgcgatggtg
     9061 ctggcaaaaa atcttctcgg caataacacg ccgctgaaac taccggcgat gctggtgaaa
     9121 atcaaaacgc cggaattacc gctgcatctg gcaggcgaaa cccagcgtca ggatttacgc
     9181 tggcaaatta ataccgaacg ccagggaatg gtggctcgcg gtgttgacga tgctgaccag
     9241 cttcgcgcct ttgtggtcag tgaggatcgg atgaaagagg catttggatt gttgaaaaca
     9301 ttgccgatgt aggtgggcta ctgtgcctaa aatgtcggat gcgacgctgg cgcgtcttat
     9361 ccgacctacg gggacgcatg tgtaggccag ataaggcgtt tacgccgcat ccggcaatgg
     9421 tgtccaaatg caacacgttt tatccgttct ggacttcacc cgctaaccaa cgcgccgcag
     9481 caataacccc ctgccccaga gacaaaccgc catcacccgc cggtaaactc tgtggaaaga
     9541 gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg ttatgaataa
     9601 ccccgccgct aaataccagc gtagtgatac cacgcatcgt ggcctgctca cgcatcaacg
     9661 cggcaaaacc ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta accggtgcct
     9721 gccagttcag ccactgctgc cagaaagcgg cgagatccag ttgattgtcc accagcggca
     9781 tcgtcaccgg atgcgtcact ccgtggcatg aggctgcgag cgcctccaga gcacaagccg
     9841 cttcaccttc ataacttaac gtggctggcg cacagcccag tgccgccgcc actgcatcga
     9901 acaaccgccc acacgatgac gccagcggcg cgttaattcc acgctcaatg gcccgcgcca
     9961 gcacgctcca gttttgctgt tgcacacttg ctgtttcagg gtaattctgc cactccggca
    10021 caaagcgcag gcactgcgcc agcaggtttc gccacggctg cttcgctgcc aaatcgccac
    10081 ccggaagcgc cactgcaggc aagccgccca ggtgctcaca ttcgcgatag ttcacccgca
    10141 ggcactcgcc gccccacaaa gcgccgttct cccccatacc gataccgtcg agcgtcaaag
    10201 caatgacatc accgccatcc aacggccact gatgctctgc cagacacgcc gctgcatggg
    10261 catgatgatg cagtaccgtt tgcgtcggca gattcatttc acgcgcccac tggctggaga
    10321 catagcccgg atgcgcgtca tgcacaacgt attgcggggt aaaatcgtag atgttttgca
    10381 tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa tcgcccagat
    10441 gctgactcaa caccgcttgt tcaccgcgca ccaggcagaa ggtatttttc agatccgcgc
    10501 cgagacacag cacaggcgga acatttttaa agcccggagg caaagccagc gcatccggca
    10561 catacccccg cgaacggcgc agcatttcgc cgctttcgcg caccaccgaa tcatccatcc
    10621 gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc aatgccctgc aaatccgcca
    10681 gcgcctgttc gttgctgata gctggtggtt taccgctcag gttgccggag gtcatcacca
    10741 gcgggcattg cagttcctgt aacagcaaat gctggagcgg gttcgcaggc aacattaccc
    10801 cgacttcgtt aaggtcaggg gcgatatcat cacaaagctc aggaacgtat tttttatcca
    10861 ccagcacaat cggcgcggcg ggcgtggtaa gcaactggcg cgcagcgtct ggtaaaccgt
    10921 cagccactgg caacatgacc gccagcggtt tcgccgggcg atgtttgcgc gcccgaagtg
    10981 tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg aaatccgcca atccctttga
    11041 tggcgacaat gttgcccatt tttaactgtg cgatagccgc ctgtaatgcc gcctcttgtt
    11101 ctgcatgttc accatgactt acccattcaa gatgtgggcc acactccggg caggccaccg
    11161 gctgggcgtg gaagcgacga tcgagcgggt cacggtactc tttgtcacaa gccggacaca
    11221 acggaaacgc cgccatcacg gtaaacgggc ggtcgtaagg catggcgcga ataatggtga
    11281 aacgcgggcc gcagtgggta cagttgataa acggataacg ataacgccgt tcgcctgggg
    11341 tattcatttc ggcaaggcaa gcagggcaag tagcggcatc cgggacaatt tgcgtattca
    11401 tggcacctcc cgcgctctgg cggatggtga actcagtggg cagttgtgac cagatgtacg
    11461 gctcacgctc gacgctatca atacgcgcca gcggcgggca gtgctgatgc aattgaacaa
    11521 gtaacgtttc cgggtcttcc cgcagccgga cttctacgcc atcgccgtca ttacagacat
    11581 cgccgtgaag atttaattgc tgtgccagct gccagacaaa cggacgaaaa ccgacgccct
    11641 gcactttgcc acgaatacgc agttggacac cgcaagatgt gttttttgcc attgagttat
    11701 tcccgccatc atgaattgcg taacccgtcc tgccggacac gactgcgtcg tatccggcag
    11761 tctcaggtcg gcgataccgc cagctccgta ttctacgaat atttccggga attcctttga
    11821 tgccagaaca gttctgtaag atttttagaa catcagcgcc gtacggcggc gtttttctgc
    11881 actcagttgt tcaagtttat tacgatcgac acaaatcagc gcatgagtcg ggcaagccgc
    11941 catacacgcc gggccgtctt cacgatggtt gcacaggtca catttattgg cttcggcttt
    12001 gtcagcccgt acattcagcc ccgcgccgct gttgcgaatc accggacgta ccaccacttc
    12061 catcgcgcca tacgggcaag ccacaacgca ggttttgcaa ccaatgcaac gttcctgcat
    12121 cacatgaaca aaccctttat cacggctgat agcaccattc gggcagacgt tagcgcacgg
    12181 tgcatcttca cactgacggc aaactgtcgc cgtggaaatg ttcacacctt taatgacatg
    12241 gatacgcggt aaaaatgttt ccggggtcag cgatgcacag tcctgatttt cctgatgaga
    12301 aaccacgcac gccacttcac aggtacagca accaatacat ttactcgcgt cagcaatgat
    12361 gaaacggttc atcaaattct ccagcaatga cagttaatgc gccgatacat tcacaaatca
    12421 tgccagtttt aattcactgt tatttaagga aattaatttc tgtaatgcag gaaaaaagat
    12481 gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt tatcgcgaaa gagcaatgag
    12541 tgagtcgcgg cggatcagtt ttccgctgaa ggttttcggc ggtgagaaat ccccgccatc
    12601 gagcataaaa atcagccgtc caataatttc ctgaatcatc tcagtcaccg gaatttttac
    12661 gctggagagc gccggaacga tatagggggc aattgcgata tcatcgaatc cgataactga
    12721 cacctgctct ggcaccgcta cgccgcgctc gtgcaacgct ttcatcgcac ctatcgccat
    12781 atcgtcgtta ctggcaacta acgcgctaaa tttagcccca cgttcgagca acgtttctac
    12841 cccttcggcc ccgctggcgg gcgtccattt accgttagcg ataagttttt cattgagcgc
    12901 aataccatgc tgcgccagcg cgtctttata cccagcaaga cgttcaatgc tggtggggga
    12961 atccatcgag ccggtaagga aagcaatctc ctgatgtccg gcgtttatca actctgccac
    13021 ggcattaaag ctggtctgtt tatgatcgca ccagacgcta tggctgctgt ttttgcgcag
    13081 gcggcgatta agcaccatta tcggctgact gtgcgcgtca atgatgtcat cgatctcatc
    13141 cacgcttaaa aaacgcgggt aaatcataat ggcgtcgcag cgcaaatcca gcagatactg
    13201 aatcgcctgg cgctcttctt cggcgctgtg tttaccatct gccaatagca actgccgccc
    13261 tttctcttcc gccattcgcg cggcatgaaa gagtaattca ctaaaataaa tgccgtggta
    13321 aagcgtgttg gtcactacca accccagcgt ctgagtactc ttcgccgaca gattgcgcgc
    13381 cagcaagttt gggcggtaac cgctctcttc taccgcctga aacacgcgat ctttagtctc
    13441 ctggctgacg tagccattac ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc
    13501 gcgcttcgcc acttccagca tcgtcgtcat cattttcatc cctttacacg caatcaacgc
    13561 agtgtactgc accgtttgcc gattgtcctt gcgcaatcgg cgggaaaaat attcaggtga
    13621 ccggtttcac aaatataaaa aatgaacaat taactctctt gcttatttag tgacaactat
    13681 tcatgatttt gtgaaaccgg tttcctaatt ccgtttcagc atcggcattt ttccgttacg
    13741 tcgactgata acaactacat ctaccctact gataacagga taaaatccga tggccaaaaa
    13801 ttatgcggcg ctggcacgct cggtgatagc ggcactgggc ggcgttgata acatctcggc
    13861 ggtcacgcac tgtatgacgc gtttgcgcgt tgttatcaaa gatgaccagc ttatcgacag
    13921 cccgacgtta aaaaccatcc ccggcgtgct cggcgtggta cgtagtgaca accagtgtca
    13981 ggtgattatc ggcaataccg tttcacaagc ctttcaggaa gtcgtcagcc tgctgccggg
    14041 agatatgcag cccgcacagc ccgtgggtaa acccaaactc acgctgcgtc gcattggtgc
    14101 ggggatcctc gatgcgctga tcggcaccat gtcaccgctg atcccggcga ttatcggcgg
    14161 atcgatggtc aaactgctgg cgatgatcct cgagatgagc ggcgtgctga caaaaggatc
    14221 gccgacctta accattctga atgtgattgg tgacggtgct ttcttcttcc tgccgctgat
    14281 ggtcgcggca tctgccgcca tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc
    14341 gggtgtgctg gtacatccga gttttattga actgatggcg aaagcggccc agggtgaaca
    14401 tgtcgaattt gccctgattc cggtcaccgc ggtgaaatac acctacacag tgatcccggc
    14461 gctggtgatg acctggtgcc tgtcatatat cgaacgctgg gtggatcgca ttacaccggc
    14521 ggtgaccaaa aacttcctca agccaatgct gattgtgttg attgccgcac cgctggcaat
    14581 cctgctgatt ggcccgattg gtatctggat cggtagcgcc atttcggcgc tggtttacac
    14641 cattcatggt tatctgggct ggctttcagt cgccattatg ggcgcgctgt ggcctctgct
    14701 ggtaatgacc gggatgcacc gcgtctttac gccaaccatc attcagacca ttgccgaaac
    14761 cggcaaagaa gggatggtca tgccgtcaga gatcggcgct aacctgtcgc tgggcggttc
    14821 atcactggcg gtggcgtgga aaacgaaaaa cccggaactg cgccagacgg cgctggctgc
    14881 ggcggcatca gccattatgg cggggatttc cgaaccggcg ttatacggcg tggcgatccg
    14941 cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt atttgcggcg cggttgccgg
    15001 tatggcgggg cttgccagcc actcaatggc agcgccaggg ttatttacca gcgtgcaatt
    15061 cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt
    15121 ggtgctgtcg tttatcctca cactgttgct cggctttgag gatattcctg ttgaggaagc
    15181 ggctgccgag gcgcgaaagc atcagagcgt acaaccgacc gtcgccaaag aagtaagtct
    15241 taattgagga tgaaaatgtc agtatttcca gaaggttttt tatggggcgg cgcgcttgcc
    15301 gccaaccagt ctgaaggtgc gttccgtgaa ggtggcaaag ggctgaccac tgtcgatatg
    15361 atcccacacg gcgagcatcg aatggcggtg aaactggggc tggaaaaacg ttttcagttg
    15421 cgcgatgacg agttttatcc cagccatgag gcgacggatt tttatcatcg ttataaagaa
    15481 gatatcgccc tgatggcaga gatgggattc aaggttttcc gtacctcaat tgcctggagc
    15541 cgtctctttc cgcagggcga tgaaatcacg cccaatcagc agggcattgc tttttatcgt
    15601 tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc tggtcacgtt gtgccacttc
    15661 gatgtgccga tgcatctggt caccgaatat ggctcctggc gtaaccgcaa gctggtggag
    15721 tttttcagcc gctacgcccg gacctgcttt gaagcatttg atggtctggt gaaatactgg
    15781 cttaccttca atgaaatcaa cattatgttg catagcccgt tctccggcgc gggtctggtg
    15841 tttgaagaag gtgaaaatca ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt
    15901 gccagtgcgc tagccaccaa aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt
    15961 atgctggcgg gcggtaactt ctacccttac agttgcaagc cggaagatgt ctgggcggcg
    16021 ctggagaaag accgggaaaa cctgtttttt atcgatgtgc aggcgcgggg cacgtatccg
    16081 gcttactctg cccgcgtatt ccgcgaaaaa ggggtaacca tcgacaaagc accgggcgat
    16141 gatgaaatcc tgaaaaacac cgtcgatttt gtctctttca gctattacgc ctcgcgctgc
    16201 gcctcggcgg agatgaacgc caacaatagc agtgcggcga acgtagtgaa atcgctgcgt
    16261 aacccgtatc tgcaagtgag cgactggggc tggggtattg atccactcgg tctgcgtatc
    16321 accatgaaca tgatgtacga ccgttatcag aagccgctgt ttctggtgga aaacggcctc
    16381 ggcgcaaaag atgaacttgc tgccaatggc gagattaacg atgactatcg catcagctat
    16441 ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag atggcattcc gctgatgggc
    16501 tacaccacct ggggctgtat tgatttagtc tccgcctcta cgggtgaaat gagcaaacgc
    16561 tacggttttg tctacgtaga ccgtgacgac gcaggcaacg gcacgctgac gcgcacacgt
    16621 aagaaatcgt tctggtggta taaaaaagtg attgccagta atggggaaga tttagagtag
    16681 gtaacagtgc cggatgcggc gtgaacgcct tatccggcct acatgtcgca cgaaccagga
    16741 aggcccgata agcgcagcgc atcgggcaat ctggggtttg gcatcagtca aaatcaaaga
    16801 aaccattccg gcttaacgcc aaatcgggct gaaagtgcct taatgtggga tatcgtcagg
    16861 gatcgttgtc cagacaaaat ctgactgacc agggatttag agccgatttc gttcttcaaa
    16921 tccgcatagg tcagattatg ttgatcaatc agcgtgcgaa gtaacgccac gccaacaggc
    16981 attgccgcaa cggctttgtc gaactctgca aactcttggt tgttgttttc atattctgca
    17041 atgcggctcg ccagaaagtc gataagtgga ttggtgtcat cttcttcaat caaatattcg
    17101 accagttcca gtgcttcacg ataatcatct tctgagtcac tacctcccag aaaggggact
    17161 gcattgacca gttctcttgt tgctttaaca gcgcgtgcag cattagcagt cattctttgt
    17221 tctctctgta gtaacgtgtc agtttgtcat actcggcgtg attagttatg tgcttcacaa
    17281 agaaccgctt attaacaaag tttatgtagg caatcaccct taggttgttc ccacctacat
    17341 ccagaacgta ccatttgttg cgatatttaa aattgtcgag gttgggaaac gccgttcgca
    17401 tctcttctgg cgtagaaaaa tccgtttcct taataacccg atacaatgcc tgaagagcca
    17461 gagcgtcgtt aggatattta cgcgcagact cttcaaatgg cgctttagaa atgatgtgca
    17521 tttttaagtt ttaacttcct tatagtaagt aaacagccac atctgcgagt tctatcctta
    17581 tgtgttcgga aactatagct catcaaatcc aagttcacaa tatgtaaaca cttgagcgcg
    17641 atataattac ccccaaaatc ctcatagcca ctctgcaaaa agtcagttct ggttcacccc
    17701 atcaagaaca tcccccttta tcaagcaacg ctgcgtctgc gccagatctt ccgtttgcgg
    17761 gtcgcgcccg gcgatgggtt tgaggacgag tttacggttg ggggtataag taaaacgcgt
    17821 gagcgcggga tagcttaact tgccaaacca gccgtgaaca cgctgccatt tggcgtcttt
    17881 tacctgatac agactggtta tccagtaacc ggaatcaaac tgcatatcca gcaattgcgt
    17941 gtgaccattg ccttgcaaat ccagcaaatc atctacgcca gttttgcttg cggtgtagaa
    18001 tcccctgggt tcaaagacac agggacggcc ttcgcgcgtg aacgtcataa gaattaaatg
    18061 tgcataagga gccagaccat taccggtact tggtaaccag ataaccagat cctgaatgcc
    18121 gttacgatca agatcggcgc gatagagttg ccctttgccc gctatgtttg ccagcatacc
    18181 cacaggcaca gaccaggatt taccggcgcg atctttaccg ttcaattgcc attcactatc
    18241 atcatttggc atcttttcaa gcgtcacgcc agcaacgccc acttttctaa aatgccaggt
    18301 ttgctcggca tcataaggca tggtcaggag aggtttcagc gcccgctttt gcgcgtattc
    18361 gctgtcactc attggcagat attcggtaaa gctggtggcc tgtgcaggca gcactaagct
    18421 caataaaaaa gcgcacatcc ctgttctgat attcatttcg ctgaattccc tgatggagaa
    18481 ttattcttct tccaccgcta actgcgcgaa gccgccattc ccttcccagc cttccagtcg
    18541 ttgataaacg gtttctaccg catctttaat cggctgagtc atcgggtagt aaaagccgac
    18601 gatatccggc tggatgccga ggaaaatcac ttcgccaatg tcttctttca actggtcgat
    18661 aagatagttc agcggcatgt tatgggtagt catcataaac atctcggcga tatcatccgg
    18721 gtcgatgatg cggatttcgc cggggtttag ccccatatca gtagcgtcaa caatcagtaa
    18781 ccgcgtaggg cgcagttcac ggatggcgac gatgtcgttt tccggtgcac taccgccatc
    18841 aatcaccacc cagttacctt tcggcgcggc ggcgcacttt tccgccagca gcgggccagc
    18901 gccatcatcg cccatcatgc tattgccaac acagagtaaa acgtcagtca cgcagtctcc
    18961 tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt aacagcattg
    19021 tactccattc cttctgctgc gaagtttgcg ctgcccgggc tttatcgaat gcgttggcca
    19081 gcatcggcac atggttaatg tcgatgacga tctcaccata tttcggcacg ccttccattt
    19141 tgcggcgggc ttcactgccc gcctccagag tggcaatcca tgccagatat tcatcccacg
    19201 ggcaggtgag cgccgcttcc aggcaatcga taaccccaag gtggtgacca atcgccaggc
    19261 tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca ataaatttac
    19321 ggctcagttg actgaacacc accttttcac tcatcggata cgcgcctctt caacaacatg
    19381 attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca gccagcgtgc
    19441 aacctgttct tcgccctgcc ctaactgtgt caggaagtca tcggcaatct gacgaccgta
    19501 acgataaccc gccaggcggc gtgcttcgcg atccactttc acacgcagcg gctgcaccat
    19561 atcgccatgc aggatttccg cctgttgttc atcctgctca cccgggccac gggcgtgaat
    19621 tttctgctcc agcaggccga gcgccattgc aaagccgtac agcgtggcgg caggcgttgg
    19681 cgggcaacca ggaatgtaga catctaccgg gacgatttta tccgtaccgc cccacacgca
    19741 gtagagatcg tggaaaatac caccgctgtt accgcaggca ccgtaggaga tacaaatttt
    19801 cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac gggtgaccgc
    19861 gccggtaaac agtaaaatat ccgcatgacg cggtgaagga acgactttaa tgccgaagcg
    19921 ttctgcatca aacagcggcg aaagcgtggc gaaaatttcg atttcgcagc cgttgcagcc
    19981 gccgcagtcc acgcgataaa cgtaggcaga acgtttgatt tttttcagta acgacgcctt
    20041 catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg cgtcacgggg
    20101 gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc aatacggtcg
    20161 gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct ttcgcggtgg
    20221 ttttccgcgc ggctgtcgcc gttgtgctta agcagcgcaa tggcgtagtc gatctctttc
    20281 tggacggcga aaggacgatt gcagacgcgg cagttgcaca gcgcgaagcg ggactgttgc
    20341 aggaagtctt ctttcttcca cactgccagt tcgtactctt gcgacagttt gatcgccgcc
    20401 gtcgggcaaa cttcttcgca gcgtccacag aagatgcagc gcccgagatt aaactcccac
    20461 gccagctcac cggtggcgag gtcagtttca accgttaagg cgtttgacgg gcaggcattg
    20521 acgcaggccg cacagccgat gcactgctgc gggttctgct ccggcttacc acggaagttt
    20581 ttatcaaccg caatcggctc cagcggataa gacgaggtcg ccgtgccggt tttgatgact
    20641 tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt acgctcaatg
    20701 ctgtaacgct cgagttcttt gtacggcacc actttgctct ttttcttacg cacatcgacc
    20761 acggtcatgc ggtcggtaca ggagtagcaa gggtccaagc taccgataat cagcggcgca
    20821 tcggaaacgg tgttaccgcg cagcatgtag cgcagggtcg gccagttcgc gtaggtcgcg
    20881 gcacggcagc gccagcggta cagcttctgg ttgtcgccgg tcatgctcca gtggatatcg
    20941 tcgccgcgcg gcgcttcggc aaagcccagc gcgaaacggt gcggaatgta ggtaaagcct
    21001 tccaccatca gcggaccgcc cggcaggtta tccagaccgt agtcgatcat gttcagcgcg
    21061 gtataaactt cgttgatacg cactttcaga cgggaaataa cgtcgcagcc ctgctcgctg
    21121 tggacttcca ttggcagcag gccataaccg acaaacgggt gatcggcgcg agtatcgcgg
    21181 gcgtgaccgc tggcgcgaac catcgggcca acgttactga aatcgcgggc catttccggg
    21241 tccagacgac caatgccgac ggtgcgctgt tccatgttcg gcgtactcag cagcacatcc
    21301 accagctcct gcacttcacg acgcatctgt tgcgccagct ggcgggtctg gatcatgtca
    21361 tctttcaaca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt tttacgcgca
    21421 ccggtgagga tctctgccat tttcatggag gtttcacgca cgcggaagaa ctgcataaag
    21481 ccggagtcaa agccggtaaa gtggcaggcg agaccgaggt tgagcagatg cgagtgcagg
    21541 cgttccacct ccagcagaat ggcgcggatc atctgcgcac gttctggcac ctgaataccc
    21601 atcgcgtttt ctaccgacgt ggtgtaggcg gtgctgtggg caaagccgca gatcccgcac
    21661 acacggtcag agaggaaggt cacttcgttg taacccatac gggtttccgc cagtttttcc
    21721 atgccgcgat gaacgtagaa cagacggtag tcggcgtcga taatgttttc gccatcgacg
    21781 aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat cggcacgacg
    21841 ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcatc ggtggtcggt
    21901 gccggacgct gacgataatc catgctgtct ttacgcagcg gataaagttc atccggcagc
    21961 accagacggc gttcatccgg cagaccaacc ggaatcaaac cgtacatatc gcgcacttcg
    22021 cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg acggatactc cggtttgttg
    22081 gcatcaactt cgacgcgaac cgttacccag cacttagtcc ccttctccat cgacagcacg
    22141 taataaacgg cgtaatgacc attcagtttg cgttcgtcgt taccaaaaag caccgacagc
    22201 cagcccccct gcttgtagta gagaaactcc accacttccg gcagatagtt cacctttacg
    22261 gtgacagtca gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc cggaaatgcc
    22321 tcattcagcg cggcgagata atgttgacct aatttttctt cagacatgct caaactctct
    22381 ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac ccagtccagg
    22441 taatgcgcgg agtaatatca agacgcagac gggccatgct gttttcgaac agcgcgataa
    22501 ccaggacgcc gacgaccagt ttgacgatgg caatcaccag cgccagcagc agcccaccgg
    22561 cggtgaaggt ttccatctgt ccccacggga taaatacgcc gacgaacatc tgcagcacca
    22621 ccagctgttt caggctgata ccccatttca tgacgccaaa gccgctgccg ctgtattcag
    22681 agagcgggcc ttcctgcagc tcctgctcgg cttccgccag gtcgaacggc agtttgccca
    22741 tttcgataaa ggtggcgaac gcacaggcac aaagcgccag taccagcggg atgctctggc
    22801 tcagcggcca gtgataaacg gtgtcggtga tgttgctgat gttggtggaa ccggcaacct
    22861 gtgcggcaac ccacagacca agcagcagca tcggttcgac cagcacgcca agcatcgctt
    22921 cacggctcgc gccgatagcg gtaaacgggc taccggtatc cagaccagaa atggcaaaga
    22981 agaaacgcgc gatggcaaag agatacagta aggtgattaa atcacccagt tgcggcagcg
    23041 gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg cccaccatca
    23101 catacggcgt caggcggaac acccagccgg aggcatccgg gccaacgctc tggcggccca
    23161 gcagtttgaa aatgtcacga tactcctgca acacacccgg cccacgacgg ttatgcagac
    23221 gggcgcgcgc cacgcgggta ataccggaga gcagcggcgc aacggcaaat aacaccagcg
    23281 cctgaattaa cggatataaa acactcattc tcaggctcct cgtgaaacaa taatcaccac
    23341 cagtaccgcc agttcaacca gcgccatccg gcggaacagc aacgcactac cttcgcactg
    23401 ccagcccggc accagcgaca ccggattcag ccatttgcgc agtttcagca ctggcgcaaa
    23461 cgcctgttta accggcatgg caaaaccgtg agcggtaatc accattgatt tttcatggtc
    23521 gtagtcgcac acccaggccg caccgcggga acgtgacggc aggcggtcgc ctttgcaaat
    23581 cgccataatg atgaatggca gcagcgggca ggcaatcagc agcaatgtga tcattggttg
    23641 agaaacggtg gtgttagcag gctccagagg cagaggtaca gcagcagaga gcatcggcag
    23701 cagccacggc gcagcaacac cgccaattac gcagcaaatc gccagtgcca ctacgcttac
    23761 gctcatcagg agcggcgcac aggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag
    23821 gaacgtgacg ccatagactt tcgccataca catcaccgcc agcgcaccgg taattgccag
    23881 acccacagcg agcagcggcc ccagcagacg ggcaacaaac gcgccgctat tgctcagttt
    23941 gaagaaggat tgatagataa cccattcccc ggcaaacccg ttcagtggcg gcagcgcagc
    24001 cattgccatc aacccgacta acatggcgat ggagataacc ggcattttct tgccaatacc
    24061 accgagtttt tcgatatcgc gatgaccggt acggaaccag acgctccccg ccccgaggaa
    24121 cagtacgctt ttgaacaggc tatggttaag cagatggtac agaccaccga ccaggccaag
    24181 agcaatcagc gccggttgtt cgagcgcgat acccgttacg ccagcgccca gccccagcag
    24241 gataatgccg atattttcca gggtgtggta agccagcagg cgctggatgt tgtgttccat
    24301 caacgcatac agaccaccga cgaacgcggt gatcatgccg agcaccagca gcgcgatccc
    24361 ccaccacagc ggtgcattac cgcccagcag tgacagggtt aacatgccca gcaggccaat
    24421 tttcatgact accgtagaaa acaacgcggc agctggcgca gaggcgttag catgtgcctg
    24481 cggcacccag ccgtgcagcg gaataatccc ggccagcagg ccaaagccaa tcacaccgag
    24541 cagccagata tcggaaccga gcggcagctg ttgcatacgc atatccagta ggcacagatc
    24601 cagcgtgcca taacgctgcc acagcagcca gcaagcaatc accagcagca gagtgccaag
    24661 acgccccagc gcaaaccaca gtttgccctc tttgctgttg ctggtgagga acaccgcaca
    24721 caaggccatg acttcggcca ttaccacgaa catgccgagg ttgctggcga tgacggcgca
    24781 gacggcggca gccatcaaca tattgatctg caagccgttg cacttcacct gcgcgtggcg
    24841 atgccagtca atgttgtaga ggctgacaaa cagaccgcac agaccgagcg taatcagcca
    24901 aatcgcgtta agcggagaga tttgcacatc gtagcttacc agcgacagcg caccgctcac
    24961 gccaaccgcg ccagtcagta cagtgaagcc cgcggctgcc gtatacagac taccaaccgc
    25021 gccgccaatt ccagctatcc agccacttaa cgctttttga aaagaaaaga gaaatgccag
    25081 aacagcggcg gcgacaaacc atgccacgcc gctattgatc agggaaattg cgctcattta
    25141 gcctctccac tttgagcctg ctgaaacaag gtgagatcgc caaagtccgt gttaaaggtc
    25201 agctcacgct tacgtttgct gacgcgggcg atatcggtgt tatccaccag atgcagggct
    25261 ttagtcgggc acatccgcac gcaggccgga ccttgttcat caaaactgca aaggtcacat
    25321 ttgacggcga tcgcgcgaat acccggcacc cagtcaagca gcgtactgat acgcgccgga
    25381 gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg ccggaatatc cagcggacgg
    25441 ctgccggaaa attcaattgc gccaaacggg caggcgatgc cgcacagttt gcagcttacg
    25501 cacagacttt cattcaactg cacggcccca tcaatgcggg tgatggcgtt aaccgggcaa
    25561 accaccgcac agggtgcatc ttcacagtgg tgacagagct gcggcgcaga ttctttttca
    25621 ttcagcatca ctctcaggcg cggcattgat tgcaggccgt gctggcgatg cgtctctgaa
    25681 caggcggcct cacaagtgtg gcagccgata cagagcgtgg agtcaacaat tacaaaacga
    25741 ttcaccaggc attcctcagg tgattgtcat ttttgacgaa aacatgccga tgaaatgtca
    25801 ttttcgacac tcatcgacac gcccatcccc aaacaggcgt aacgcctgca aaacgggcaa
    25861 agcctcagct catgctgccg ggctttgtcc ctttaccagt tggcttaaat tcaccggcac
    25921 attgttttca atagcggtgt ataagctgtc gtaaacgcca gcgatttttt cgagatagtg
    25981 ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca tcaatcatgc cgtcaatgct
    26041 cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct tttgtttcga cgtaatactc
    26101 gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa atacgaaaat gttcaaaaag
    26161 gacgaaacag agttctttgt ccggcgcgca gctcatggcg tgatgcaggc gcgctttcga
    26221 taacgtgatc ccctgaacca gcgaattgca gtagatgtgc cactggtcct gtaggcgaca
    26281 atgccgctgt gcgatgtaat cggctttctc gcttatttcc caaatagtca ttgtcaggtt
    26341 acccgtttaa cagagatgcc agctttaagc attttctatg ccaactttta atttattgtt
    26401 attaaataga tttttaagct aaagatgaat ttcgtcgccg tgtcgacgtg tcatttcgac
    26461 atcatcgaca ttattcaccg cagggataat caacactggc acaattattg cttgtagctg
    26521 gcaatagtta atgggaggcg atatgcacga aataaccctc tgccaacggg cactggaatt
    26581 gatcgaacag caggccgcaa aacacggcgc aaaacgcgta actggggtct ggctcaaaat
    26641 tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt tgttttgatc tggtttgccg
    26701 cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa caagaggccg aatgctggtg
    26761 tgaaacatgc caacagtatg tgacgctact gacccagcgc gtccgccgct gtccacagtg
    26821 tcatggtgac atgctacaga ttgtggcaga cgacggttta cagattcggc ggatagaaat
    26881 agaccaggag tgagcgatgt gtacaacatg cggttgcggt gaaggcaacc tgtatatcga
    26941 gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg ccatttgccc cggcggcacg
    27001 cccgaagatg aaaatcaccg gcattaaagc gcctgaattt acccccagcc agactgaaga
    27061 aggcgacctg cattacggtc atggcgaagc gggcactcac gcgccgggca tgagccagcg
    27121 tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaat aaccgtctgg ctgaacgcaa
    27181 ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac ctggtttcca gccctggttc
    27241 cggtaaaacc accctgctga cggaaaccct aatgcgcctg aaagacagcg ttccgtgcgc
    27301 ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca cgcattcgcg ctaccggcac
    27361 accagcgatt caggtgaaca ccggtaaagg ctgccatctt gacgcacaga tgattgccga
    27421 cgccgcaccg cgtctgccac tggacgataa cggtattctg tttatcgaaa acgttggcaa
    27481 cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac aaagtggcgg tgctttccgt
    27541 taccgaaggt gaagacaaac cgctgaaata tccgcatatg tttgccgccg cctcgctgat
    27601 gctgctcaac aaagttgacc tgttgccgta tctcaacttt gacgttgaga agtgcatcgc
    27661 ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt atttccgcca ccagcggcga
    27721 agggatggac cagtggctga actggctgga gacacagcga tgtgcatagg cgttcccggc
    27781 cagatccgca ccattgacgg caaccaggca aaagtcgacg tctgcggcat tcagcgcgat
    27841 gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc agccgcgcgt gggccagtgg
    27901 gtactggtac acgttggctt tgccatgagc gtaattaatg aagccgaagc acgcgacact
    27961 ctcgacgcct tacaaaacat gtttgacgtt gagccggatg tcggcgcgct gttgtatggc
    28021 gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc ggaacaggtg atgcagttaa
    28081 ttgagcatct gcgcgaacgt gcttcacatc tctcttacac cgccgaacgc cctctgcgga
    28141 ttatggaagt gtgcggtggt catacccacg ccatttttaa attcggcctc gaccagttac
    28201 tgcctgaaaa cgttgagttt atccacggtc cgggttgccc ggtgtgcgta ctgccgatgg
    28261 gtagaatcga cacctgcgtg gagattgcca gccatccgga agtcatcttc tgtacctttg
    28321 gcgacgccat gcgcgtgccg gggaaacagg gatcgctgtt gcaggcaaaa gcacgcggtg
    28381 ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa actggcgcag gagaatccaa
    28441 cccgtaaagt cgtattcttc ggcttaggtt ttgaaaccac catgccgacc accgccatca
    28501 ccctgcaaca ggcgaaagcc cgcgatgtgc agaattttta cttcttctgc cagcatatta
    28561 cgcttatccc gacgctgcgc agtttgctgg aacagccgga taacggtatc gacgcgttcc
    28621 tcgcgccggg ccacgttagt atggttatcg gcactgatgc ctataatttt atcgccagcg
    28681 attttcatcg tccgctggtg gtcgctggtt tcgaacccct tgatctactg caaggcgtgg
    28741 tcatgctggt ggagcagaaa atagcggccc acagcaaggt agagaatcag tatcgtcggg
    28801 tggtaccgga tgccggtaac ctgctggcgc aacaggcaat tgctgatgtg ttctgtgtca
    28861 acggcgacag cgaatggcgc ggcttaggcg tgattgaatc ttctggcgtg cacctgacgc
    28921 cggattatca acgattcgat gccgaagcac atttccgccc agcaccgcag caggtctgcg
    28981 atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa atgtaagccg catcaatgcc
    29041 cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg tgcactgatg gtctcctccg
    29101 aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga gaatgaagcg tgaataatat
    29161 ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa ttaatcaaca gcctgtttat
    29221 ggaagccttt gccaacccgt ggctggcaga gcaggaagac caggcacgtc ttgatctggc
    29281 gcagctggta gcagaaggcg accgtctggc gttctccacc gacagttacg tcattgaccc
    29341 gctgttcttt cctggcggta atatcggcaa gctggcgatt tgcggcacag ccaatgacgt
    29401 tgcggtcagt ggcgctattc cgcgctatct ctcctgtggc tttatcctcg aagaaggatt
    29461 gccgatggag acactgaaag ccgtagtgac cagcatggca gaaaccgcgc gcgcggcagg
    29521 cattgccatc gttactggcg atactaaagt ggtgcagcgc ggcgcggcag ataaactgtt
    29581 tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt cactggggcg cacagacgct
    29641 aaccgcaggc gatgtattac tggttagcgg cacgctcggg gaccacgggg cgactatcct
    29701 taacctgcgt gagcagctgg ggctggatgg cgaactggtc agcgactgcg cggtactgac
    29761 gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa gcgctgcgtg atgccacccg
    29821 tggtggtgtg aacgcggtag ttcatgagtt cgcggcagcc tgcggttgtg gtattgaaat
    29881 ttcagaagtg gcactgccgg ttaaacctgc cgtgcgcggc gtttgcgaac tgctgggact
    29941 ggacgccctt aactttgcca acgaaggcaa actggtgatc gccgttgaac gcaacgcggc
    30001 agagcaagtg ctggcagcgt tacattccca tccactgggg aaagacgcgg cgctaattgg
    30061 tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg tatggtgtga aacgaaccct
    30121 cgatttacca cacgccgaac ctcttccgcg tatatgctaa taaaattcta aatctcctat
    30181 agttagtcaa tgaccttttg caccgctttg cggtgctttc ctggaagaac aaaatgtcat
    30241 atacaccgat gagtgatctc ggacagcaag ggttgttcga catcactcgg acactattgc
    30301 agcagcccga tctggcctcg ctgtgtgagg ctctttcgca actggtaaag cgttctgcgc
    30361 tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac tcaacgtgcg tcttattacg
    30421 catcgcgtga aaaagacacc ccattaaata tgaagacgaa actgttctgg cacacggtcc
    30481 ggtacgccgc attttgtcgc gccctgatac gctgcattgc agttacgaag aattttgtga
    30541 aacctggccg cagctggtcg caggtgggct ataccccaaa tttggtcact attgcctgat
    30601 gccactggcg gcggaagggc atatttttgg tggctgtgaa tttattcgtt atgacgatcg
    30661 tccctggagc gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt
    30721 caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac tatgagttgt tatgccggga
    30781 gcgcgataac ttccgcatcc tggttgccat taccaacgcg gtgctttccc gcctggatat
    30841 ggacgaactg gtcagcgaag tcgccaaaga aatccattac tatttcgaca ttgacgatat
    30901 cagtatcgtc ttacgcagcc accgtaaaaa caaactcaac atctactcca ctcactatct
    30961 tgataaacaa catcccgccc acgaacagag cgaagtcgat gaagccggaa ccctcaccga
    31021 acgcgtgttc aaaagtaaag agatgctgct gatcaatctc cacgaacggg acgatttagc
    31081 cccctatgaa cgcatgttgt tcgacaccta gggcaaccag attcaaacct tgtgcctgtt
    31141 accgctgatg tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa
    31201 agtgtttacc actaccaatc tgaatttgct gcgccagatt gccgaacgtg tggcaatcgc
    31261 tgtcgataac gccctcgcct atcaggaaat ccatcgtctg aaagaacggc tggttgatga
    31321 aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt gaatttggcg agattattgg
    31381 ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag
    31441 taccgtgctg atcctcggtg aaactggcac gggtaaagag ctgattgccc gcgcgatcca
    31501 taatctcagt gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc
    31561 cggattgctg gaaagcgatc tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc
    31621 ccagcgcatt ggtcgttttg aactggcgga taaaagctct ctgttcctcg acgaagtggg
    31681 cgatatgcca ctggagttac agccgaagtt gctgcgtgta ttgcaggaac aggagtttga
    31741 acgtctcggc agcaacaaaa tcattcagac ggacgtacgt ttaattgccg cgactaaccg
    31801 cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc gatctctatt accgcctgaa
    31861 cgtattcccg attcacctgc cgccactacg cgagcgtccg gaagatattc cgctgctggc
    31921 gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc
    31981 cgagacgctg cgcaccttga gcaacatgga gtggccgggt aacgtacgcg aactggaaaa
    32041 cgtcattgag cgcgcggtat tgctaacacg cggtaacgtg ctgcagctgt cattgccaga
    32101 tattgtttta ccggaacctg aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga
    32161 agatgaatat cagttgattg tgcgcgtgct gaaagaaacc aacggcgtgg ttgccgggcc
    32221 taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg
    32281 gctgggaatt gataaatcgg cattgattta actgcaaatt gccggacaga tctgcctgtc
    32341 cggcatacta ttcacgaggt tttttcggac gatatttttc cggcagttct ggtaccggac
    32401 gcttgtcatc gatgagatga cgcacggtta agatcggatg acgccacagc attctcggcc
    32461 cggcccaacg cataatctgt ttcatctctt cacgctttgc aggctggtaa cagtgcaccg
    32521 gacactgctt acaggctggt ttctcttcgc cgaacacaca tttatccagc cgcttttgcg
    32581 cgtaaacaaa caacgcctcg taatgctccg gctccgctga cgcctgcggg catttcgctt
    32641 gataaagatc gatcattttt ttaatcgtca gtttttcacg agagatacgc ttgccggaca
    32701 tgctgcctcc atactcttaa ggtgtattta tattacaact taatttgaaa ggggactatg
    32761 gctctgaagg aggaggttta ttcaaccgac gcaattttgc gtacttgctt cataagcatc
    32821 acgcaaaagc tgcaaaacag catctttccc ggaaccagca tcaagaactc gccgttcgct
    32881 tcttcccctg aaatgattaa ctccggtatc atgtgcgcct tatgtgatta caacgaaaat
    32941 aaaaaccatc acaccccatt taatatcagg gaaccggaca taaccccatg agtgcaatag
    33001 aaaatttcga cgcccatacg cccatgatgc agcagtatct caggctgaaa gcccagcatc
    33061 ccgagatcct gctgttttac cggatgggtg atttttatga actgttttat gacgacgcaa
    33121 aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg cggtgcttcg gcgggagagc
    33181 cgatcccgat ggcggggatt ccctaccatg cggtggaaaa ctatctcgcc aaactggtga
    33241 atcagggaga gtccgttgcc atctgcgaac aaattggcga tccggcgacc agcaaaggtc
    33301 cggttgagcg caaagttgtg cgtatcgtta cgccaggcac catcagcgat gaagccctgt
    33361 tacaggagcg tcaggacaac ctgctggcgg ctatctggca ggacagcaaa ggtttcggct
    33421 acgcgacgct ggatatcagc tccgggcgtt ttcgcctgag cgaaccggcc gaccgcgaaa
    33481 cgatggcggc agagctgcaa cgcactaatc cggcggaact gctgtatgca gaagatttcg
    33541 ccgagatgtc gctgattgaa ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg
    33601 aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg cacccgcgat ctggtcggtt
    33661 ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga
    33721 aagataccca acgcacgacc ctgccgcata ttcgttctat caccatggaa cgtgagcagg
    33781 acagcatcat tatggatgcc gcgacacgtc gtaacctgga aattactcag aacctggcgg
    33841 gcggtgcgga aaatacgctg gcttctgtgc tcgactgcac cgtcacgccg atgggtagtc
    33901 gtatgctgaa acgctggctg catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc
    33961 agcaaactat tggcgcattg caggatttca ccgccgagtt gcagccggta ctgcgtcagg
    34021 tcggcgacct ggaacgtatt ctggcacgtc tggctttacg aactgctcgc ccacgcgatc
    34081 tggcccgtat gcgccacgct ttccagcaac tgccggagct gcgtgcgcag ttagaaactg
    34141 tcgatagtgc accggtacag gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg
    34201 atctgctgga gcgagcaatc atcgacacac cgccggtgct ggtacgcgac ggtggtgtta
    34261 tcgcatcggg ctataacgaa gagctggatg agtggcgcgc gctggctgac ggcgcgaccg
    34321 attatctgga gcgtctggaa gtccgcgagc gtgaacgtac cggcctggac acgctgaaag
    34381 ttggctttaa tgcggtgcac ggctactaca ttcaaatcag ccgtgggcaa agccatctgg
    34441 cacccatcaa ctacatgcgt cgccagacgc tgaaaaacgc cgagcgctac atcattccag
    34501 agctaaaaga gtacgaagat aaagttctca cctcaaaagg caaagcactg gcactggaaa
    34561 aacagcttta tgaagagttg ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga
    34621 gcgcgagcgc gctggcggaa ctcgacgtgc tggtgaacct ggcggaacgg gcctataccc
    34681 tgaactacac ctgcccgacc ttcattgata aaccgggcat tcgcattacc gaaggtcgcc
    34741 atccggtagt tgaacaagta ctgaatgagc catttatcgc taacccgctg aatctgtcgc
    34801 cgcagcgccg tatgttgatc atcaccggtc cgaacatggg cggtaaaagt acctatatgc
    34861 gccagaccgc actgattgcg ctgatggcct acatcggcag ctatgtaccg gcacaaaaag
    34921 tcgagattgg acctatcgat cgcatcttta cccgcgtagg cgcggcagat gacctggctt
    34981 ccgggcgctc aacctttatg gtggagatga ctgaaaccgc caatatttta cataacgcca
    35041 ccgaatacag tctggtgtta atggatgaga tcgggcgtgg aacgtccacc tacgatggtc
    35101 tgtcgctggc gtgggcgtgc gcagaaaatc tggcgaataa gattaaggca ttgacgctgt
    35161 ttgccaccca ctatttcgag ctgacccagc taccggagaa aatggaaggc gtcgccaacg
    35221 tacatctcga tgcgctggag cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg
    35281 gcgcggcgag caaaagctac ggtctggcgg ttgctgctct ggcgggcgta ccgaaagagg
    35341 ttattaagcg cgcacggcaa aaactgcgtg agctggaaag catttcgccg aacgccgccg
    35401 cgacgcaagt ggatggtacg cagatgtctt tgctgtcagt gccagaagaa acttcgcctg
    35461 cggtcgaagc actggaaaat ctcgacccgg attcactgac tccgcgtcag gcgctggagt
    35521 ggatttatcg cttgaagagt ctggtgtaat aacaattccc gatagtcttt tgctatcggg
    35581 aatattaacg acaactgacg aatcaaataa aaataccctg tataatagga aagcttattt
    35641 tacagggtaa aaccatgcca tctacacgct atcaaaaaat caatgcccat cactatcgcc
    35701 atatatgggt cgttggtgat attcatggtg aatatcagtt attacaatcc cgttaacatc
    35761 aactctcttt ttaccctgaa accgacttac ttatttccac aggcgataat attgatcgtg
    35821 ggccgaagag tcttaacgtg ctgcgcctgc taaaccaacc ctggtttacg tcggttaaag
    35881 gcaaccacga agcgatggcg ttggatgcat tcgaaactgg cgatggaaat atgtggcttg
    35941 ccagcggtgg tgactggttt ttcgatttaa atgattcaga gcaacaagag gcaatagatc
    36001 tgttgctgaa attccatcac cttccacata ttattgaaat cattaatgac aacataaaat
    36061 atgccat
//
LOCUS       DAZSBF010000013        36029 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZSBF010000013 DAZSBF010000000
VERSION     DAZSBF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36029
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.013"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHXWD_000482"
     CDS             complement(36..1334)
                     /gene="eno"
                     /locus_tag="ACHXWD_000482"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR7619502.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHXWD_000483"
     CDS             complement(1422..3059)
                     /gene="pyrG"
                     /locus_tag="ACHXWD_000483"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR7619503.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHXWD_000484"
     CDS             complement(3287..4078)
                     /gene="mazG"
                     /locus_tag="ACHXWD_000484"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR7619504.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHXWD_000485"
     CDS             complement(4149..4484)
                     /gene="mazF"
                     /locus_tag="ACHXWD_000485"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR7619505.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHXWD_000486"
     CDS             complement(4484..4732)
                     /gene="mazE"
                     /locus_tag="ACHXWD_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR7619506.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHXWD_000487"
     CDS             complement(4810..7044)
                     /gene="relA"
                     /locus_tag="ACHXWD_000487"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR7619507.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHXWD_000488"
     CDS             complement(7092..8393)
                     /gene="rlmD"
                     /locus_tag="ACHXWD_000488"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR7619508.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLCLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8450..11206
                     /gene="barA"
                     /locus_tag="ACHXWD_000489"
     CDS             8450..11206
                     /gene="barA"
                     /locus_tag="ACHXWD_000489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR7619509.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHXWD_000490"
     CDS             complement(11438..12778)
                     /gene="gudD"
                     /locus_tag="ACHXWD_000490"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR7619510.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12799..14139)
                     /locus_tag="ACHXWD_000491"
     CDS             complement(12799..14139)
                     /locus_tag="ACHXWD_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR7619511.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHXWD_000492"
     CDS             complement(14141..15493)
                     /gene="gudP"
                     /locus_tag="ACHXWD_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR7619512.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15928..16377)
                     /locus_tag="ACHXWD_000493"
     CDS             complement(15928..16377)
                     /locus_tag="ACHXWD_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR7619513.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHXWD_000494"
     CDS             complement(16395..17177)
                     /gene="truC"
                     /locus_tag="ACHXWD_000494"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR7619514.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17177..17506)
                     /locus_tag="ACHXWD_000495"
     CDS             complement(17177..17506)
                     /locus_tag="ACHXWD_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR7619515.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHXWD_000496"
     CDS             complement(18128..18673)
                     /gene="syd"
                     /locus_tag="ACHXWD_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR7619516.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18741..19589
                     /gene="queF"
                     /locus_tag="ACHXWD_000497"
     CDS             18741..19589
                     /gene="queF"
                     /locus_tag="ACHXWD_000497"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR7619517.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHXWD_000498"
     CDS             19701..21065
                     /gene="ppnN"
                     /locus_tag="ACHXWD_000498"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR7619518.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHXWD_000499"
     CDS             21621..22910
                     /gene="sdaC"
                     /locus_tag="ACHXWD_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR7619519.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHXWD_000500"
     CDS             22968..24335
                     /gene="sdaB"
                     /locus_tag="ACHXWD_000500"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR7619520.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNASRMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24447..25202
                     /gene="xni"
                     /locus_tag="ACHXWD_000501"
     CDS             24447..25202
                     /gene="xni"
                     /locus_tag="ACHXWD_000501"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR7619521.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHXWD_000502"
     CDS             complement(25307..26455)
                     /gene="fucO"
                     /locus_tag="ACHXWD_000502"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR7619522.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHXWD_000503"
     CDS             complement(26483..27130)
                     /gene="fucA"
                     /locus_tag="ACHXWD_000503"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR7619523.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27677..28993
                     /gene="fucP"
                     /locus_tag="ACHXWD_000504"
     CDS             27677..28993
                     /gene="fucP"
                     /locus_tag="ACHXWD_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR7619524.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29026..30801
                     /gene="fucI"
                     /locus_tag="ACHXWD_000505"
                     /pseudo
     CDS             29026..30801
                     /gene="fucI"
                     /locus_tag="ACHXWD_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30910..32328
                     /gene="fucK"
                     /locus_tag="ACHXWD_000506"
     CDS             30910..32328
                     /gene="fucK"
                     /locus_tag="ACHXWD_000506"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR7619525.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32330..32752
                     /gene="fucU"
                     /locus_tag="ACHXWD_000507"
     CDS             32330..32752
                     /gene="fucU"
                     /locus_tag="ACHXWD_000507"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR7619526.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32810..33541
                     /gene="fucR"
                     /locus_tag="ACHXWD_000508"
     CDS             32810..33541
                     /gene="fucR"
                     /locus_tag="ACHXWD_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR7619527.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHXWD_000509"
     CDS             complement(33585..34685)
                     /gene="rlmM"
                     /locus_tag="ACHXWD_000509"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR7619528.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34678..35073)
                     /locus_tag="ACHXWD_000510"
     CDS             complement(34678..35073)
                     /locus_tag="ACHXWD_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR7619529.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHXWD_000511"
     CDS             complement(35092..36009)
                     /gene="gcvA"
                     /locus_tag="ACHXWD_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR7619530.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT         8824 a         9573 c         8677 g         8955 t
ORIGIN      
        1 ggaggctggc atttttaaat cagataaagt cagtcttatg cttggccttt gatctcttta
       61 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
      121 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
      181 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
      241 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
      301 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
      361 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
      421 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agatacgatc
      481 ggatactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaaagctttg
      541 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
      601 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
      661 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
      721 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
      781 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
      841 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcatg
      901 gagtatttgc ccggagtgcc gttcagttca gcgatgtgct cgtacagcgg catcccttta
      961 tcggcagcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     1021 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     1081 ttagcgtctt tgccaatcag tgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     1141 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     1201 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     1261 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     1321 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     1381 acgcgtaccc aaggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     1441 tggaactcgc tggcagcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     1501 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     1561 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     1621 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     1681 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     1741 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     1801 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     1861 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     1921 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     1981 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     2041 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     2101 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     2161 tcaatgtact tgccgaccat accgatggtg acttcactta ccggattcgc ttcttcgaag
     2221 ataacctgtt cccattcgga cagatttgct tccgggcagt ttaagctgaa tcgtttacaa
     2281 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     2341 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     2401 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     2461 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     2521 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     2581 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     2641 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     2701 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     2761 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     2821 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     2881 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     2941 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     3001 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     3061 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     3121 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     3181 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     3241 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     3301 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     3361 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctcg aatttttcgt
     3421 tcgctttttg caatgcgatt tctgctttcg tccctaaatg gcgagccaga ttaaccgtgg
     3481 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     3541 cctcgtacat cacctcgtcg atctcttcgt agactttatc aaccaccggg ccaagcgtcg
     3601 tccagtcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcccgc attaaagccg
     3661 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     3721 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     3781 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     3841 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     3901 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     3961 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     4021 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     4081 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     4141 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     4201 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     4261 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     4321 gattgcgttg tacaaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     4381 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     4441 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     4501 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttgacca
     4561 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     4621 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     4681 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     4741 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     4801 aatttctgcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     4861 tttacccagc acgcggccca gcacctgcag gttgtaaatc tcaatggtca tgtcgatggt
     4921 tgccagttgc tgttttgtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     4981 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     5041 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     5101 cgcatgagag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     5161 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     5221 gtggtgcatc aggttgccaa caccttcgac taccacgcga ccgttatctt tactgcggtt
     5281 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     5341 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     5401 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     5461 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     5521 cccagccaga atgtttttgt cacggtcctg tttacggaac caagcgtgaa ttttcgaacg
     5581 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     5641 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     5701 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     5761 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     5821 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     5881 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     5941 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     6001 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     6061 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     6121 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcatagca
     6181 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcaaaggc
     6241 gaggttcttt ttctgcattt tacgccagat gctgtagatg tgtttcggac gaccatacac
     6301 ttccgcttta acgccttcag ctttcatctc agcgcgcaga tgaccaacga actcttcgat
     6361 gtagtgttcg cggtcgagac gccgttcatg cagcagtttg gcaattcgtt tgtattcggt
     6421 tggatggagg taacggaagc agtaatcttc cagttcccat ttcagttgtc cgattccgag
     6481 acggttagcc agcggtgcgt agatgttggt acactctttt gccgccagta cacgttcatc
     6541 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgactac
     6601 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     6661 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     6721 gtgaataagg ttaacaaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     6781 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     6841 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     6901 cccctgcgtc tgttgcagac aatacgccca agtttcggct aagcactcac acgacttctg
     6961 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     7021 atgtgcactt cttaccgcaa ccatcgtcct ctcctttagg gaccagacct gccgaaatcg
     7081 gcaaatcgca actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     7141 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     7201 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     7261 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     7321 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     7381 aacgtcacat tctgtaagcc attaagacgc gcattctgct ggcctttttc caccagcgcc
     7441 ggaacgcctt ctacacccac cacactggca gcttgtgtcg ccaatggcag tgtaaagttg
     7501 cccataccgc agaacaaatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     7561 gcacgcgcta ccattttttg gttcacaccc gcattgacct gaataaaatc gcgcgggcta
     7621 aaagttaagc acaacccatt tgagtcatac cagggcatct caccagagac ggtttcgagt
     7681 atctcactat cgggggcgag atacagatcc aggccttcag aatgcgaaaa gcgttccagt
     7741 ttttcgcgat ctgccgaact tagcggtgcg gtatggcgca aaatcatcag cgtgccgctg
     7801 gttgcctgta ccagttcaac atgaccaaga tggcgcataa cttgtaagct gcccaagcat
     7861 gccatgactt tgggcagcaa tgcttcaagt tggggcacta aaatggggca ttgtttaacg
     7921 tcgacaatgt cactggagcc cgctttgcga aaccccatct gaagttgctg tgttttcggt
     7981 aagtagttca gacttaaacg cgcgcgacgg cgatagcccc agggaacatc ggcgatcact
     8041 tcagagactt cgtgtttcat taatcgggcg agtgccgcac ttttgcttcg ctgctgtaaa
     8101 tccacgctgg cgtgttgttg ctgacagccg ccgcatacgc caaaatgagg acagcgtggc
     8161 gtttcgcgtc ccgggctatc gcttaaccgg cgtacgactt tagcgcgggc atactgtttt
     8221 ttatcttcag taacagtaac ttccgcgttt tcctgcggca ataatccggg gataaatagc
     8281 gttttgccgt tatgtcgcgc cacgccctga ccaaaagagt cgaggtcgtt gactgaaacg
     8341 gttatgatct gacgcgtcgt cgtgcgtcgt tttgcagagt agaattgcgc cattggcgag
     8401 actttctcaa tttaacagtg tgaccttaat tgtcccataa cggaactcca tgaccaacta
     8461 cagcctgcgc gcacgcatga tgattctgat cctggcaccg accgtcctta ttggtttatt
     8521 gctgagtatc tttttcgttg tgcatcgcta taacgacttg cagcgtcaac tggaagatgc
     8581 cggagccagc attattgagc cgcttgcagt ttctactgaa tatggcatga gcctgcaaaa
     8641 tcgcgaatct atcggtcagt taataagcgt actgcatcgt cgccattccg atattgttcg
     8701 cgcgatttcg gtttatgatg aaaataaccg actctttgtc acctccaatt ttcatcttga
     8761 tccctcatca atgcagctcg gcagcaacgt gccgtttcct cgccagctca ctgtcactcg
     8821 tgacggcgat attatgatcc tccgcacgcc gattatttct gagagttact cccccgacga
     8881 atcgcccagt agcgatgcca aaaatagtca aaatatgttg ggatatattg cgctggagct
     8941 ggatcttaaa tcggttcgct tgcagcaata taaagagatc tttatttcca gcgtgatgat
     9001 gctgttttgt atcggtattg cgcttatttt tggctggcgc ttaatgcgcg atgtaaccgg
     9061 tccgattcgc aacatggtga ataccgtcga ccgcatccgt cgcgggcaac tcgacagccg
     9121 ggtggaagga tttatgctcg gcgagctgga tatgctgaaa aacggtatca actcgatggc
     9181 aatgtcgctg gctgcttatc acgaagagat gcagcacaat atcgaccagg cgacgtccga
     9241 tctgcgtgaa acgctggagc agatggaaat tcagaacgtt gagttagatc tggcgaaaaa
     9301 gcgcgcccag gaagcggcgc gtattaaatc cgagtttctg gcaaatatgt cacacgagct
     9361 gcgtacacca ctgaatggtg ttattggctt tacccgcctg acgctgaaaa cagaattaac
     9421 accaacgcag cgcgatcacc tgaatacgat tgaacgttcg gcaaataatt tgctggcaat
     9481 tattaatgat gttctcgact tctcgaaact ggaagcaggt aagctgattc tggaaagtat
     9541 tccattccca ctacgcagca cgctggatga agtcgttact ctgctggcac attcttctca
     9601 cgataaaggg ttggaactga cgctcaatat taaaagcgac gtgcctgata acgtgatcgg
     9661 cgacccactg cgattacagc aaatcatcac taacctggtg gggaatgcaa ttaaattcac
     9721 cgagaatggc aacattgata ttctggtaga aaaacgtgcg ctgagtaata ccaaagtgca
     9781 gattgaagtg cagattcggg ataccggcat tggtattcct gaacgcgatc aatcgcgctt
     9841 attccaggcc ttccgacagg ctgatgccag tatttcccgc cgtcatggtg gcaccggtct
     9901 ggggctggtg attacacaaa aactggttaa tgaaatgggc ggcgatattt cgttccatag
     9961 ccagccgaat cgcggttcaa ctttctggtt ccacattaat ctcgatctga acccgaacat
    10021 tattatcgaa gggccatcca cccagtgcct cgccggtaag cgcctggcct atgtcgaacc
    10081 aaactccgca gcagcgcaat gcacgctgga tattttaagt gaaacgccgc tggaagtggt
    10141 ttatagccca acgttctccg cgctgcctcc cgcgcattac gacatgatgt tgttaggcat
    10201 cgcggtgacc ttccgcgagc cgctaacaat gcaacatgag cgattagcga aagcggtatc
    10261 gatgaccgat ttcctgatgc tggcacttcc ttgccatgca caagtcaatg cagaaaaact
    10321 caagcaagat ggtatcggcg cgtgtctgct gaaaccatta acacctacgc gcctgttgcc
    10381 tgccctgacg gaattttgtc atcacaaaca aaacacgctt ttgcctgtaa ccgatgaaag
    10441 caagctggca atgacagtca tggcagttga tgacaacccc gctaacctga aacttatcgg
    10501 cgcattgctg gaagatatgg tgcaacatgt ggaactttgc gatagcgggc atcaggcggt
    10561 tgaacgggcg aaacagatgc cgttcgattt gatcttaatg gatattcaaa tgcctgacat
    10621 ggatggcatt cgggcctgcg agctcatcca ccagctcccc catcagcaac aaacgccggt
    10681 tatcgcggta acggcgcatg caatggccgg gcaaaaagag aagctgcttg gcgcagggat
    10741 gagcgattat ctggcgaaac cgattgaaga agagcgattg cataatttgt tgttgcgcta
    10801 caagcctggc agcggtattt cctctcgcgt cgtgacgccc gaagtcaacg aaattgtggt
    10861 gaacccgaat gcgaccctcg actggcaact ggcactacgc caggcagcag gaaaaaccga
    10921 tctagcgcgc gatatgctgc aaatgttact cgatttcctg cctgaagttc gcaacaaagt
    10981 tgaggagcag ctggttggag aaaacccgga aggcctggtt gatttgattc ataaactgca
    11041 tggcagttgc ggctatagcg gtgtgccgcg tatgaagaat ctctgccaac tgatcgaaca
    11101 acagctacgt agcggtacta aagaagaaga tttggagccg gagctgctgg aactgttgga
    11161 cgagatggat aatgtcgcgc gcgaagccag caaaattctc gggtaacgga cgtcttgtcg
    11221 agtagcaatc ggctttttat gacgctggtt tcagattggt gacaaagtgc ctgtgtttat
    11281 gccggatgcg gcgttaacgc cttatccggc ctacaaaacc aattaaattc aatgaattaa
    11341 aaaatgatgt agacctgata catagcacaa caggctattt tgcgtttagc atcagtctca
    11401 agccggctcc agatagagcc ggttttggtt ttctgtctta acgcaccatg cacgggcgct
    11461 tgttatcgaa cgtccagcca ggaatcagat actgcattcc catcgcatcg tcacgcgcgc
    11521 caagcccgtg tttctgatac agctcatggg ctttcatcac ttgatccata tcgatttcta
    11581 cacccagccc cggtttttcc ggcacctgaa ccagaccgcc tttgatctca aacggttctt
    11641 tggtcaagcg ctgattgcct tcctgccaaa tccagtgcgt atcaatcgcg gtgattttac
    11701 ccggtgcagc ggcggcaaca tgggtaaaca tcgccaggga aatatcgaag tggttgtttg
    11761 agtgtgaacc ccaggtcagg ccaaattcat ggcacatttg cgccacacgt accgaacctt
    11821 gcatagtcca gaaatgcgga tccgccagcg ggatatcaac ggattgtaac gagagcgtat
    11881 ggcccatctg ccgccagtcg gtggcgatca tattggttgc agtcggcagg ccagtcgcgc
    11941 gacggaactc tgccatcact tcacgcccgg agaaaccttg ctccgcacca tacggatctt
    12001 ctgcataagc cagcgaacct ttcaggtatt taccaatttt aattgcttcg ttcagcgacc
    12061 aggcaccgtt aggatcgagc gtaatacgcg cctgcgggaa gcgttgcgcc agtgccacaa
    12121 tagactcggc ttcttcttcc ccggccagta caccgccttt cagtttgaaa tcgttgaagc
    12181 catatttttc atacgccgct tccgctaggc gcaccaccgc atccggcgtc atcgcttctt
    12241 catgacgcag gcgataccag tcgcatgagt catccggctg gctttgatac ggcagaggtg
    12301 tggctttgcg attaccgacg aagaacagat aaccgagcat ttcgacttcg ctacgctgtt
    12361 gaccatcgcc cagcagcgat gccacgttta cccccagatg ctgccccagc agatccagca
    12421 ttgccgcttc tatcccggta actacatgaa tagtggtacg caaatcaaat gtctgcaaac
    12481 cgcgcccacc agcatcacga tcggcaaaag tattacgcac cagcgtcaga acgtttttgt
    12541 attcacccag cgttttacct accaccagcg gaatcgcatc ttccagcgtt ttacggattt
    12601 tctcgccgcc gggaatttcc cctacgccag tgtgaccgga attatctttg ataatcacaa
    12661 tattacgcgt aaagaacggt gcgtgtgccc cactcagatt catcagcata ctgtcatgac
    12721 ccgccaccgg gatgacctgc atttcagtaa caacagacgt cgtaaattga gaactcatat
    12781 ttatgtcctt tttcagaatc aatgacggcc gaaaacggga cgtttacggt caaaggtcca
    12841 gccggggatc aggtactgca tcggacctgc gtcgttacgc gcaccgccag gcagacgttt
    12901 ataggcctca tgtgcctttt gtacctgttc ccagtccagt tccacgccca gaccgggcgc
    12961 atcaggaacg gcaatttttc cgtttttaat ctccagcgga tttttggtca ggcgacaatc
    13021 gccctcctgc caaatccagt gggtatcgat agcggtagga ttacccggtg ccgccgcgcc
    13081 cacatgggta aacatcgcca gagagatatc gaaatggtta ttagaatggc agccccaggt
    13141 cagcccccag tcgtcgcaaa gctgcgccac acggactgca ccggaaagcg tccagaagtg
    13201 cggatcggca agtggaatat ctaccgcatt gagcatcacc gcatgaccca tttcgcgcca
    13261 gttggtggcg atcatgttag tcgcgacggg caagccggtc gcccgccgaa actccgccat
    13321 aacttcacga ccggagaaac cctgttctgc gccgcatgga tcttcggcat aggtaagaac
    13381 atcattcagc cctttgcata gagaaatggc ttcatcaagt agccaggcac cgttgggatc
    13441 aacggtaatc cgcgcatcag ggaagcgttt cttcaatgca cgaacagtgt cgatttcttg
    13501 ctcgccaggt aacaccccgc ccttaagttt gaaatcttta aagccatagc gatcctgtga
    13561 ggcttccgcc agacgcacaa cggcttcgct gttcatcgct ttctgatggc gcaactgata
    13621 ccactcatgg ttgcccggcg tattttccag ataaggaaga tcggttttgg tccgatcacc
    13681 gatataaaac agataaccga ggacggtaat agtctcgcgt tgcttgcctg gccccaacag
    13741 ttcgcagacc ggaacattca gcgccttacc tagcaggtca agcaaggcgg cttccagcgc
    13801 cgccacggca ttaacgcgca attcaaaagt ccaggcacct ttgccgaagg tatcaaaatc
    13861 ggctgcctga ttacctttat gcacctgctg aaccacttta ttcaggcgcg caacttcctg
    13921 gcccagaacc atcggaatag catcgaccag tgtttgataa atcacctctc cgcccggcgc
    13981 ttcaccaatg ccggtatgcc cggcgttatc ggtgagtacc acaatattgc gggtgaaata
    14041 tgcgttatgt gccccaccaa tattaagcaa catgctatcg tgcccggcga ccggaatgac
    14101 tttcatatca gtaataacag gactggattg tgtcgtcatc ttattgcccc gcgacaggtt
    14161 tcagctcgat acgcttgata tcgcccacca gcaccaggta gctcagtacc gcgattaagg
    14221 catgaacacc aacataaatc agcgccccat taaaggagcc agtcgtgcca acgatataac
    14281 caattgcgat tggcgtgacg atgccagaaa tgttaccgaa catgttgaac aggccaccgg
    14341 aaagaccgct gatctctttt ggcgcggtat ctgccattac tgcccaaccc agcgccccga
    14401 tgcccttacc gaagaaggcc agcgccataa agccgatgat catccactca acgttgacgt
    14461 agttgcagaa caccatcacc atcgacagca acatgcccat tacgatcggt gttttacgcg
    14521 caatgttcag cgatcccgtg cgacgcatca gccagtcgga aataatccca cccagcacac
    14581 cgccgataaa accgcaaacc gccggaacgg aagccacaaa gcccgctttc agaatcgaca
    14641 tcccacgcgc ctgcaccaga taaaccggaa accaggtaat gaaaaagtaa gtcagggcgt
    14701 tgatacagta ctgaccgata taaacgccga tcatcatccg tgacccgagc agttgtttga
    14761 tctgccccca cttcacgctg aacggcactt taactttggt gttttgctga tccatattga
    14821 tcagcgcgcc acccgcggcg atgtactcca gctctttctg atttaccccc ggatgttgat
    14881 ttggctcgtg gatgactttc aaccagataa agctgatgac aatccccaga ccgcccataa
    14941 agaagaagac gtgtgaccag cccacttcat gcgtcagcca gcccataatc ggcgcgaaga
    15001 tcaccgttgc gaagtattga gcggagttaa aaatcgacac cgccgttccc ctttcctgcg
    15061 ccggaaacca ggccgcaaca atgtgactgt tgccggggaa agatggcgct tcagcaagcc
    15121 cgaccaggaa gcgcagagta aacagggcaa cgataatgcc gaatccacta aagatatcga
    15181 cgaagccttg cagcaaggta aacatcgacc agataaagat cgaccagaag tagacgcgtt
    15241 ttgaaccaaa acgatccagc aaccaaccac cagggatctg cccgataaca taagcccatg
    15301 agaaggcaga gaacacatag cccattccca cgggatcaag gccgatatct ttggccattt
    15361 ccgaaccggc aatagaaagc gtagcgcggt cgccgtagtt gaaggatgtg acgataaaca
    15421 acatcaccac tatccagtaa cgagcattcg tgcgtttttc cacactgctc gcagcctgac
    15481 ttaaagaact cattgttgca ctcctgaaaa ttcgcgttag ccacgctcac tctggactgc
    15541 gacatagcca ggaaatcaga ggtgacgtag ggtgtttttt gccgttttta taggtcgttc
    15601 gccgaatacg gcgcgtgttt atatctggca atagcagtat aaaaagcgcg ccatagcggc
    15661 tcaccgtgca acaacacaac attaatgcgt tcaatgaggc ccgattttgg cattagccct
    15721 ggacggtgga atccacttca cggaaatgaa aacaagaaca agaaaggaag ggttaaaacg
    15781 aagaaataaa gaagagtatg aaatggatcg cttgactcca ggcaaacgcc agtaaaaatc
    15841 cgcgctatga agcagttttt actggcattt gcctgaaaag attcgattca gcaccgctaa
    15901 aacgacattt accgctcgct gaacatatca ggacaacagc gtgccccact gttcgaccca
    15961 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16021 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16081 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    16141 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    16201 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    16261 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    16321 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    16381 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    16441 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    16501 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    16561 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    16621 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    16681 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    16741 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    16801 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    16861 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    16921 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    16981 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17041 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    17101 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    17161 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    17221 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    17281 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    17341 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    17401 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    17461 cttcaagcgt ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacttcaat
    17521 aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta ttcacagtgc
    17581 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    17641 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    17701 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    17761 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    17821 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    17881 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    17941 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18001 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18061 gcttatttcc tggcgatctc ctacagggtc tgtaagagat ctctcacaac cagatggggt
    18121 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    18181 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    18241 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    18301 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    18361 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    18421 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    18481 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    18541 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    18601 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    18661 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    18721 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    18781 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    18841 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    18901 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggtcg
    18961 ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg aagagtttta
    19021 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19081 cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg ttatatcgtc
    19141 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    19201 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    19261 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    19321 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    19381 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    19441 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    19501 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    19561 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    19621 gcagaaggtt gtgaaaggac atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    19681 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    19741 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    19801 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    19861 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    19921 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgcgttgc
    19981 aggccaactt gttcgcggtt ctgcgtgaca ttctcttcgt ttacgggcaa atccataaca
    20041 ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac ctggtctttt
    20101 ccatcttgcg taacgctcgc gcactgcatg tcggtgaagc gccaaatatg gtggtctgct
    20161 ggggcggaca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc gggaatcagc
    20221 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    20281 tgaaaggtgc tgcggtcgga cacgcacagc agcgttacaa agacagtcgt tttattggta
    20341 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    20401 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggcatcatta
    20461 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    20521 acccggccaa caaagatcag gttttaccat tgttcctcac cggcccgaaa gagagcgccg
    20581 actacttccg cgtactggac gagtttgtcg tgcatacgtt gggtgaaaac gcgcgccgcc
    20641 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    20701 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    20761 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    20821 agctttaccc ggatcaacct gttgaagtgc tggctgccga tctgcgccgt gcgttctccg
    20881 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    20941 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21001 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21061 cgtaatctcc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacatacc
    21121 catcaattta tttcatttgt tatatgaatg tttcttacca ccctcacgcg ataaatatca
    21181 ttgcagttaa tatgtcatac aatttatgtt gtaacgcaaa cgtttcccta ttttcataaa
    21241 ccacatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    21301 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    21361 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    21421 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    21481 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    21541 atttcatttt tacggtcagg caccttcccg agctgaactg gctaaaagct gaattatttg
    21601 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    21661 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    21721 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    21781 tcatcatggc tatccttgcg ttcccgatga cattttttgc tcaccgcggc ctgactcgct
    21841 tcgtactgtc tggtaaaaac ccaggcgaag acatcaccga ggttgtagaa gaacactttg
    21901 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    21961 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    22021 tgacaccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    22081 ttcgcttcgg tgagcagatg atcgttaaag caatgagtat tctggtattc ccgtttgttg
    22141 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    22201 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    22261 caattccggt tatggtgttc tctttcaacc actctccgat catctcttct ttcgccgttg
    22321 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    22381 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgtgta ctgagcctga
    22441 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    22501 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    22561 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    22621 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    22681 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    22741 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    22801 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    22861 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    22921 gccgctttcg ggcggcgctt cctccgtttt aacacgatgt atttcctatg attagcgtat
    22981 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    23041 cgggtaaaca atttaccgac gatctgattg cccgtaatct gcttaaagac gtgacccgcg
    23101 tggtggttga cgtgtacggc tcgctctctc tgaccggtaa aggccaccac actgatatcg
    23161 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    23221 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    23281 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    23341 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    23401 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    23461 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    23521 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    23581 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    23641 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    23701 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    23761 acatgtttgc actggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    23821 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagttcatcc
    23881 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    23941 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    24001 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    24061 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    24121 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    24181 acgcctcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    24241 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    24301 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    24361 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    24421 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatgcactga
    24481 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    24541 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    24601 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    24661 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    24721 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    24781 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    24841 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    24901 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    24961 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    25021 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    25081 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    25141 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    25201 aacggcgagc cggatgcaaa cgattatcaa cacattgaat ttaatgcact tatataccgg
    25261 ataagacgcg gctagcgtcg tatccggcat tatcacatca gcgcatttac caggcggtat
    25321 ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg gtacaaacat
    25381 catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacacca acatcacgca
    25441 aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca gcggcattac
    25501 gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg atatcgcggt
    25561 acttctcacc ggtaaagtca gcgttatagt gcatgacatg cggtaacagg atggcgttcg
    25621 caacaccgtg tggagtgttg taaaacgcgc ccagtggatg cgccatacca tgcaccaacc
    25681 ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc atttcttctc
    25741 cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt tcaatcgctt
    25801 taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc tcaatagcat
    25861 gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca tccatcatgt
    25921 cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca aacttgcgcc
    25981 gtttctcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagta cctgctgtgg
    26041 taggaattgc cagaatcggt acactgggtt tattggtcgg ggaaagccct tccaggctac
    26101 gcacatcggc aaactccgga ttgttgctga taatgccaat cgctttacaa gtatcctgtg
    26161 gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat acaccgagcc
    26221 cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc gcccatgcca
    26281 gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc accagcgttt
    26341 tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg gttaaagccc
    26401 caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta gccatcatcc
    26461 ttctccttgt tgctttacga aattactctt caatccgtaa cccataggtt ttgaatttct
    26521 ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta atcgccaggg
    26581 tcgccacgta aagttgcgcc agcacttcaa cttcatgcgc cagccataac gctttttcca
    26641 gattcacctc acaagcgata agcccatgat gttgtaacaa agttgcctta cgatttttga
    26701 gagccagcgc aacatgttca gaaagttcgc gtgttccaaa ggtcgcataa ggcgcgcaag
    26761 gaatagaatt accacctgcc gccgcaatca tgtagtgaat agcggggatc ggtcggttaa
    26821 gaatggaaac tgctgtgcaa tgaacggcat gattgtgaac aaccgcgttg gcatccggtc
    26881 tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt ccttcctcat
    26941 gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca tatggaatgc
    27001 ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc gctgtcccct
    27061 ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga gcaagtttat
    27121 ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg ttcctttcac
    27181 actattgaat tagccgttta attacccacc atcttcttcc tgattaacaa gaaagaaatt
    27241 cacaagctta tattttgtga cctagctcaa ctaatcacag taaataactg caagttctct
    27301 ttttataacc ccattaaaaa tgaccgctct taaaaatatt tatcaaaacg gtcatttttc
    27361 tattcaccca agccaggaat gcccgttttc ggcataaaca attaatacag tcatctggtt
    27421 tgtgtttttt atgatttatt ttctgaaacg ggcatgaaat ttcgattatt aaagtgatgg
    27481 tagtcacata aagtcacctt ccagctaata agtgtgaccg ccgtcatatt acagagcgtt
    27541 ttttatttga aaatgaatcc atgagttcat ttcagacagg caaatattca ctgatatgaa
    27601 gcccgaactc gctggttttg cacttttgaa aacataaccg attacgtgct taagcttctg
    27661 aacctaagag gatgctatgg gaaacacatc aatacaaacg cagagttacc gtgcggtaga
    27721 taaagatgca gggcaaagca gaagttacat tattccattc gcgctgctgt gctcactgtt
    27781 ttttctttgg gcggtagcca ataaccttaa cgacatttta ttacctcaat tccagcaggc
    27841 ttttacgctg acaaatttcc aggctggcct gatccaatcg gccttttact ttggttattt
    27901 cattatccca attcctgctg ggatattgat gaaaaaactc agttataaag caggaattat
    27961 tactgggtta tttttatatg ccctgggcgc tgcattattc tggcccgctg cagaaataat
    28021 gaactacacc ttatttttag ttggcctatt tattattgca gccggattag gttgtctgga
    28081 aactgccgca aacccttttg ttacggtatt agggccggaa agcagtggtc acttccgctt
    28141 aaatcttgcg caaacattta actcgtttgg cgcaattatc gcggttgtct ttgggcaaag
    28201 acttattttg tctaacgtgc cacatcaatc gcaagacgtt ctcgataaaa tgtctccaga
    28261 gcaattgagt gcgtataaac acagcctggt attatcggta cagacacctt atatgatcat
    28321 cgtggctatc gtgttactgg tcgccctgct gatcatgctg acgaaattcc cggcattgca
    28381 gagtgataat cacagtgacg ccaaacaagg atcgttctcc gcatcgcttt ctcgcctagc
    28441 gcgtattcgc cactggcgct gggcagtatt agcgcaattc tgctacgttg gcgcacaaac
    28501 ggcctgctgg agctatttga ttcgctacgc tgtagaagaa attccaggta tgactgcagg
    28561 ctttgccgct aactatttaa ccggaaccat ggtgtgcttc tttattggtc gtttcaccgg
    28621 tacctggctc atcagtcgct tcgcaccaca caaagtcctg gccgcctacg cattaatcgc
    28681 tatggcactg tgcctgatct cagccttcgc tggcggtcat gtgggcttaa tagccctgac
    28741 tttatgcagc gcctttatgt cgattcagta cccaacaatc ttctcgctgg gcattaagaa
    28801 tctcggccag gacaccaaat acggttcgtc cttcatcgtt atgaccatta ttggcggcgg
    28861 tattgtcact ccggtcatgg gttttgtcag tgacgcggcg ggcaacatcc ccactgctga
    28921 actgatcccc acactctgct tcgcggtcat ctttatcttt gcccgtttcc gctctcaaac
    28981 ggcaactaac tgaacatatt ttccgaataa agtgaggaat ctgtaatgaa aaaaatcagc
    29041 ttaccgaaaa ttggtatccg cccggttatt gacggtcgtc gcatgggtgt tcgtgagtcg
    29101 cttgaagaac aaacaatgaa tatggcgaaa gctacggccg cactgctgac cgagaaactg
    29161 cgccatgcct gcggagctgc cgtcgagtgt gtcatttccg atacctgtat cgcgggtatg
    29221 gctgaagccg ctgcttgcga agaaaaattc agcagctaga atgtaggcct caccattacg
    29281 gtaacgcctt gctggtgcta tggcagtgaa accatcgaca tggatccaac ccgcccgaaa
    29341 gccatttggg gctttaacgg cactgaacgt cccggcgctg tttacctggc agcggctctg
    29401 gcagctcaca gccagaaagg catcccggca ttctccattt acggtcatga cgttcaggat
    29461 gccgatgaca catcgattcc tgccgatgtt gaagaaaaac tgctgcgctt tgcccgcgcc
    29521 ggtttggccg tcgccagcat gaaaggtaaa agctatctgt cgctgggcgg cgtttcgatg
    29581 ggtatcgccg gttctattgt tgatcacaac ttctttgaat cctggctggg aatgaaagtc
    29641 caggcggtgg atatgaccga actgcgtcgc cgtatcgatc agaagattta cgacgaagcc
    29701 gaactggaaa tggcactgac ctgggctgat aaaaacttcc gctatggcga agatgaaaat
    29761 aacaaacagt atcaacgcaa tgccgagcaa agccgcgcag ttctgcgcga aagtttactg
    29821 atggcgatgt gtatccgcga catgatgcaa ggcaacagca aactggccga tattggtcgc
    29881 gtggaagaat cacttggcta caacgccatc gctgcgggct tccaggggca acgtcactgg
    29941 accgatcaat atcccaatgg tgacaccgcc gaagcgatcc tcaacagttc atttgactgg
    30001 aatggcgtgc gcgaaccctt tgtcgtggcg accgaaaacg acagtcttaa cggcgtggca
    30061 atgctaatgg gtcaccagct caccggcacc gctcaggtat ttgccgatgt gcgtacctac
    30121 tggtcaccag aagcaattga gcgtgtaacg gggcataaac tcgatggact ggcagaacac
    30181 ggcatcatcc atttgatcaa ctcaggttct gctgcgctgg acggttcctg taaacaacgc
    30241 gacagcgaag gtaacccgac gatgaagcca cactgggaaa tctctcagca agaggctgac
    30301 gcttgcctcg ccgctaccga atggtgcccg gcgatccacg aatacttccg tggcggcggt
    30361 tactcttccc gcttccttac cgaaggcggc gtcccgttca ccatgactcg tgtcaacatc
    30421 atcaaaggcc tgggatcggt actgcaaatc gcggaaggct ggagcgtgga attgccgaag
    30481 gatgtgcatg acatcctcaa caaacgcacc aactcaacct ggccaaccac ctggtttgca
    30541 ccgcgcctca ccggtaaagg gccgtttacg gatgtgtact cggtaatggc gaactggggc
    30601 gctaaccatg gggttctgac catcggccac gttggcgcag actttatcac tctcgcctcc
    30661 atgctgcgta tcccggtatg tatgcacaac gttgaagaga ccaaagtgta tcgtccttct
    30721 gcctgggctg cgcacggcat ggatattgaa ggccaggatt accgcgcttg ccagaactac
    30781 ggtccgttgt acaagcgtta atacattact ccctgatgtg atgcccggtc gctccggcta
    30841 ccgtgcctga acaagcaaga gtggttagcc ggataagcaa tgttatccgg ctatattgca
    30901 ggagcgatta tgaaacaaga agttatcctg gtactcgact gtggcgcgac caatgtcagg
    30961 gccatcgcgg ttaatcggca gggaaaaatt gttgcccgcg cctcaacgcc taatgccagc
    31021 gatatcgcga tggaaaacaa cacctggcac cagtggtctt tagacgccat tttgcaacgc
    31081 tttgctgatt gctgtcggca aatcaatagt gaactgactg aatgccacat ccgcggtatc
    31141 gccgtcacca cctttggtgt ggatggcgct ctggtagata agcaaggcaa tctgctctat
    31201 ccgatcatta gctggaaatg tccgcgaaca gcagcggtta tggacaatat tgaacagttg
    31261 atctccgcac agcggttgca ggctatttct ggcgtcggag cctttagttt caatacgtta
    31321 tataagttgg tgtggttgaa agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg
    31381 ctctttattt cgtcgctgat taaccaccgt ttaaccggcg aattcactac tgatatcacg
    31441 atggccggaa ccagccagat gctggatatc cagcaacgcg atttcagtcc gcaaatttta
    31501 caagccaccg gtattccacg ccgactcttc cctcgtctgg tggaagcggg tgaacagatt
    31561 ggtacgctac agaacagcgc cgcagcaatg ctcggcttac ccgttggcat accggtgatt
    31621 tccgcaggtc acgataccca gttcgccctt tttggcgctg gtgccgacca aaatgaaccc
    31681 gtgctctctt ccggtacatg ggaaatttta atggtccgta gcgcccaggt tgatacttcg
    31741 ctgttaagtc agtacgccga ttctacctgc gaactggata gccaggcagg gttgtataac
    31801 ccaggtatgc aatggctggc atccggcgtg ctggaatggg tgagaaaact gttctggacg
    31861 gctgaaacac cctggcaaat gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat
    31921 ggtgtgaaaa tgcagtgtga tttattgtcg tgtcagaacg ctggctggca aggagtgacg
    31981 cttaatacca cgcgggggca tttctatcgc gcggcgctgg aagggttaac tgcgcaatta
    32041 cagcgcaatc tacagatgct ggaaaaaatc gggcacttta aagcctctga attattgtta
    32101 gtcggtggag gaagtcgcaa cacattgtgg aatcagatta aagccaatat gcttgatatt
    32161 ccggtaaaag ttctcgacga tgccgaaacg accgtcgcag gagctgcgct gttcggttgg
    32221 tatggcgtag gggaatttaa cagcccggaa gaagcccgtg cacagattca ttatcagtac
    32281 cgttatttct acccgcaaac tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac
    32341 aatttcgccg ttaatttctc ccgaactatt gaaagtgctg gcagagatgg gacatggaga
    32401 tgaaattatt ttttccgatg ctcactttcc cgcccattcg atgggaccgc aggtgatccg
    32461 cgctgatggc ctgttggtga gcgacttgct ccaagcgatt atcccattat ttgagctgga
    32521 cagttatgcc ccgccgctgg tgatgatggc agcggtagaa ggtgacactc tcgatcctga
    32581 agtagaacga cgttaccgta atgcgctttc actacaagcc ccgtgtcctg acatcatccg
    32641 catcaatcgt tttgcgtttt atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg
    32701 cgaacgagcg aagtacggga atattctttt aaaaaaaggg gtaacaccgt aatctcatat
    32761 cggtacgccc gcatgacgcg ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc
    32821 acgccagcaa gcgatagtcg acctgctgct gaaccatacc agcctgacca cggaagctct
    32881 ctctgaacag ctaaaggtca gtaaagaaac cattcgtcgc gatctcaatg aattacagac
    32941 gcagggtaaa attctgcgca atcatggacg cgctaaatat atccaccgtc aaaatcaaga
    33001 cagtggcgat ccctttcaca tcaggctgaa aagccattat gcgcataaag cagatatcgc
    33061 gcgcgaggcg ctcgcgtgga ttgaagaagg gatggtgata gccttagacg ccagttcaac
    33121 ttgctggtat ctggcacgcc agttgcctga catcaacatt caggtcttca ccaatagcca
    33181 tccgatttgc catgaactcg gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac
    33241 acttgagcgc aaatatggct gttacgtcaa tccctcgctg atttcccaac ttaaatcgct
    33301 ggaaatcgat ctgtttattt tttcttgtga agggatcgat agcagcggcg cactgtggga
    33361 ctccaatgcg atcaacgctg attacaaatc gatgctatta aaacgtgccg cgcaatcgtt
    33421 gttattgatt gataaaagta aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga
    33481 tgaggtaacg cacattattt ctgatgagcg ccaggttgca acttctttgg taacagcctg
    33541 acggaaaggg tagcaggccg aagacgacgc cccggccttg cctgttatcg ctcgtcgcga
    33601 cgaccaccca ccgcagccca gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga
    33661 tacaactgcc gcgcctgaat ctgagcattt atgccatgtt catcaagctg tgcctgaata
    33721 tacgccagat tgtgtgatac ttcttcgtag cgttttttca tcggcagttt gagattgaaa
    33781 atggtttcac ggcaccagcc attaaccagc cactgcgcca tcaatgccgc aactttagcc
    33841 ggtttttcaa ccatatcgca taccatccag gagatattgc tgcgtgtcgg acggaatttg
    33901 aaaccgtctt cccgcagcca cgtcacctgc ccggtatcca tcagactttg cgccatcggg
    33961 ccgttgtcga cggaataaac ccacatgttg cgcttcacca gttggtaggt ccagccgcca
    34021 gggcaagcgc ctaaatccac cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc
    34081 gcaggaataa acacatgaaa tgcctcttcc agtttgagcg tggaacgact cggcgcctct
    34141 gccggaaatt tcaggcgcgg aatgcccata tagaacggcg aattattgtt gctgtatgag
    34201 taaccggtat agcagcagcc tggtgcaata aagaatacat gcacaaccgg acgcttcggc
    34261 gtttcatagt tcgccagcac cccggcatcg cgcaaggcag cgcgtagcgg cacggtgaat
    34321 ttacggcaga atttcagcaa ctctttgctt tcgttggtat cggcaacttc aacacgcagt
    34381 tcaccgccct tctctactac gccctgcaac atgtcgacaa tgggggtaat acgatcttct
    34441 ggcggcaaat gctgcaagag ttcccccacc acaaaccact ggcgggcaaa aattaatgaa
    34501 ctgaacggca gctcacggat taacttatcg ccatcatcag gttgataaca ttcataaatg
    34561 acataacccg cattctcttt cacgcgggca aaaccgaaaa tttcccgctg gccggcttta
    34621 tcggtaattt ctgcggcgca ctctttttca aagcccggac ggcacagcaa tacaacctta
    34681 ttcatgactt acgcccttgc gctttaaacg gatagcacca actaacatta acgcccagcc
    34741 cgcgaggaag ctcacgccgc caaccggtgt gacaaatgcc cacaaacgca gatgggacag
    34801 cgccaggcaa taaaggctgc cgctgaacaa caccgtgcct aacgcgagga aaacgctact
    34861 ccagtaaaac cagatactga tgcgacgctg cattgccacc gccagaccta agatcgccag
    34921 cgtatgaaac gcctggtatt cgaggccggt ctggatccag cccatctcaa cggcccccat
    34981 ggttttactt aacacatgcg cgccaaaagc gcccagagcc acaaaaatga agccgctaat
    35041 ggcggcgaaa atcagcataa aacggctggt catggtcgta ccctacgtaa attattgttc
    35101 ataacgaaag cggaattttt cttgttcagc agcggctttc gccaggatcc attggcgaaa
    35161 ggcggctatt ttacccagtt ctgcctggct gtcatgacaa accagataaa aagcgttttt
    35221 actgaccaga acatcattaa acgggcaaac aagacgtccg gcctcgattt cagattgcgc
    35281 catcacgtta tttgccagcg ccactccctg cccgtgaata gccgcttgca gcaccatggc
    35341 gctatgacta aaaattggcc cttgctgaac gttgatatga tttaacccca actgtcgggt
    35401 atatgtctgc cagtcacggc gtgacgcatc atgtaataac gtatgtttag ccagatcttc
    35461 cggggtcttc aagggttttt cgcctgtcag cagtagcggc gaacacaccg gcaataaata
    35521 ttcggcgtac agtttttcca cccgtagccc cggccagttg ccccgaccat aaaatatcgc
    35581 cacatcaaca tcatccgcca gcttatcttc ctgacgatca accgcctgga ttcgaacgtc
    35641 aattcccgga taagctgaat taaagctgga aagtcgcgga accaaccaat gaatggcgaa
    35701 actggggagt aaactgaccg tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt
    35761 cgcttcggtt aattgcgaaa atatctcttt gatatcgagg aaatagcttt gcccttcctc
    35821 ggtcagcagg agtgaacgat tacggcggcg gaacagtttt agccccaaaa aatcctcaag
    35881 agacttgatt tgatgactta ctgcggcctg ggtcacgaaa agctcttctg ctgcgcgagt
    35941 gaaacttaaa tggcgtgctg cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg
    36001 tttagacatg gcttttaaac tttgatgtt
//
LOCUS       DAZSBF010000014        35894 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZSBF010000014 DAZSBF010000000
VERSION     DAZSBF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.014"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHXWD_000512"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHXWD_000512"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR7619531.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3122)
                     /locus_tag="ACHXWD_000513"
                     /pseudo
     CDS             complement(1496..3122)
                     /locus_tag="ACHXWD_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(3581..3667)
                     /locus_tag="ACHXWD_000514"
     tRNA            complement(3581..3667)
                     /locus_tag="ACHXWD_000514"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3631..3633),aa:Leu,seq:gag)
     gene            complement(3682..4014)
                     /gene="secG"
                     /locus_tag="ACHXWD_000515"
     CDS             complement(3682..4014)
                     /gene="secG"
                     /locus_tag="ACHXWD_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR7619532.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="ACHXWD_000516"
     CDS             complement(4242..5579)
                     /gene="glmM"
                     /locus_tag="ACHXWD_000516"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR7619533.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5572..6420)
                     /gene="folP"
                     /locus_tag="ACHXWD_000517"
     CDS             complement(5572..6420)
                     /gene="folP"
                     /locus_tag="ACHXWD_000517"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR7619534.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="ACHXWD_000518"
     CDS             complement(6510..8444)
                     /gene="ftsH"
                     /locus_tag="ACHXWD_000518"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR7619535.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="ACHXWD_000519"
     CDS             complement(8544..9173)
                     /gene="rlmE"
                     /locus_tag="ACHXWD_000519"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR7619536.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9299..9592
                     /gene="yhbY"
                     /locus_tag="ACHXWD_000520"
     CDS             9299..9592
                     /gene="yhbY"
                     /locus_tag="ACHXWD_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR7619537.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9748..10224)
                     /gene="greA"
                     /locus_tag="ACHXWD_000521"
     CDS             complement(9748..10224)
                     /gene="greA"
                     /locus_tag="ACHXWD_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR7619538.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10472..11905
                     /gene="dacB"
                     /locus_tag="ACHXWD_000522"
     CDS             10472..11905
                     /gene="dacB"
                     /locus_tag="ACHXWD_000522"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR7619539.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11960..12067
                     /locus_tag="ACHXWD_000523"
                     /pseudo
     CDS             11960..12067
                     /locus_tag="ACHXWD_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="ACHXWD_000524"
     CDS             complement(12091..13263)
                     /gene="cgtA"
                     /locus_tag="ACHXWD_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR7619540.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13279..14244)
                     /locus_tag="ACHXWD_000525"
     CDS             complement(13279..14244)
                     /locus_tag="ACHXWD_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7619541.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="ACHXWD_000526"
     CDS             complement(14371..14628)
                     /gene="rpmA"
                     /locus_tag="ACHXWD_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR7619542.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="ACHXWD_000527"
     CDS             complement(14649..14960)
                     /gene="rplU"
                     /locus_tag="ACHXWD_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR7619543.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15219..16190
                     /gene="ispB"
                     /locus_tag="ACHXWD_000528"
     CDS             15219..16190
                     /gene="ispB"
                     /locus_tag="ACHXWD_000528"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR7619544.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHXWD_000529"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHXWD_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR7619545.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHXWD_000530"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHXWD_000530"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR7619546.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHXWD_000531"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHXWD_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR7619547.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHXWD_000532"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHXWD_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR7619548.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHXWD_000533"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHXWD_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR7619549.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHXWD_000534"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHXWD_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR7619550.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHXWD_000535"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHXWD_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR7619551.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLANCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHXWD_000536"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHXWD_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR7619552.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="ACHXWD_000537"
     CDS             21785..22762
                     /locus_tag="ACHXWD_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR7619553.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHXWD_000538"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHXWD_000538"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR7619554.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHXWD_000539"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHXWD_000539"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR7619555.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="ACHXWD_000540"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="ACHXWD_000540"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR7619556.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="ACHXWD_000541"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="ACHXWD_000541"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR7619557.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="ACHXWD_000542"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="ACHXWD_000542"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR7619558.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHXWD_000543"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHXWD_000543"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR7619559.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="ACHXWD_000544"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="ACHXWD_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR7619560.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHXWD_000545"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHXWD_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR7619561.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHXWD_000546"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHXWD_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR7619562.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="ACHXWD_000547"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="ACHXWD_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR7619563.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHXWD_000548"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHXWD_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR7619564.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHXWD_000549"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHXWD_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR7619565.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHXWD_000550"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHXWD_000550"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR7619566.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHXWD_000551"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHXWD_000551"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR7619567.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="ACHXWD_000552"
     CDS             complement(34631..35560)
                     /locus_tag="ACHXWD_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR7619568.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTFTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
BASE COUNT         9056 a         9598 c         8601 g         8639 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aatcttctag caaatttgtt gaatactgtc
     3121 atccagaaat ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat
     3181 cagacatgtt tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt
     3241 aaatgatgcg gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca
     3301 ttacgcagaa cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag
     3361 taaacatatc cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca
     3421 gcagaaggga aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta
     3481 cgagtaaagc aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa
     3541 aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt
     3601 gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca
     3661 cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc
     3721 tggctgagtt tgttcggttt tcgccggtgc actcagattt tcccattcgc tacctttatt
     3781 ggttttgttg ctattgatgt tacccagcac cagactgatg ataaagaata acgttgccag
     3841 cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga
     3901 agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag
     3961 accaacaagg ccaattgcca caataaggaa aactaccaaa agagcttcgt acataatcaa
     4021 cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa
     4081 tctttcccac tgaagtgggt gtgaatacta accaaagcga atgaccttcg caagggcaat
     4141 tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa
     4201 aaggcgacaa tcgccgcctt tttagccagt tatttaccgt tttaaacggc ttttactgca
     4261 tcggcgatac ggtgtgcaaa ttcagttacc tgcgcttcgt cttcaccttc taccatcacg
     4321 cgaattaacg gttcggtgcc ggatttacgc aacagcacac gtccacggct acccagcgcc
     4381 gcttcaactt cggcggtcac agctttaact gactcatgct caagcggatc gccgctacct
     4441 gcggtgtaac gcacgttaac cagaatctgc gggaacattt tcatgccgct gcaaaggtcg
     4501 tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg
     4561 tcaccggtag tggttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc
     4621 cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat
     4681 ggaattccta actgtttcaa cgccagctca agccccatgt tactcatcaa tgtacccaca
     4741 gcaccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga
     4801 tcaccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc gtcgccgtcg
     4861 aaggcaatac cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg
     4921 gtagccccca cttcggcatt gatgtttaca ccgttcggct cacaaccgat agcgataacg
     4981 ttcgccccca gttcacgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcgcaa
     5041 tccaccacaa tcttcagttc gctgaggcta agttcgttcg ggaacgtggc tttgcaaaac
     5101 tcgatatagc gacccgcagc atcaacgata cggctggctt tacccagttc tgccgaatca
     5161 acacagctga tctctttttc catttccgct tcgatggcct cttctaccgc atccggtagt
     5221 ttggtgccgt cgatagagaa aaatttaatg ccattatcgt agaacgggtt atgcgatgca
     5281 gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt
     5341 ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct
     5401 gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt acgggagccg
     5461 tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc
     5521 ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata
     5581 gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc
     5641 tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga ccgcacaggc
     5701 cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt
     5761 tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag
     5821 taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt
     5881 ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac
     5941 ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat
     6001 atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga
     6061 aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac ggatgacttc
     6121 tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac
     6181 aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga
     6241 ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg catgtttcac
     6301 cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag
     6361 gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa agagtttcat
     6421 ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg
     6481 gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg
     6541 ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg
     6601 tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga
     6661 cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct
     6721 ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta
     6781 cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt
     6841 ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc gtacagcagt
     6901 ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag gttggtcgca
     6961 actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct
     7021 gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg
     7081 attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc
     7141 gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct
     7201 tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca
     7261 cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg
     7321 ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc accggagaaa
     7381 ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg tacgcgacgc
     7441 atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac cacaacctga
     7501 cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg gttagtcgcg
     7561 gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc
     7621 agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg
     7681 gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg
     7741 tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata
     7801 gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt tttaccggta
     7861 cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg
     7921 ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc gtcgcagccc
     7981 gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta
     8041 ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccac
     8101 acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt
     8161 tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc
     8221 ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt
     8281 tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta
     8341 gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc
     8401 agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta
     8461 acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca
     8521 aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc
     8581 cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga
     8641 atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca
     8701 cctggcgcta atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg
     8761 gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg
     8821 actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg
     8881 ccctgaagaa agtctacacc aacgatagga tccataggta aaagatcgca agcgatgatg
     8941 cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca
     9001 aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt
     9061 ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta
     9121 aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt
     9181 tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat
     9241 atatgggaga tggcggtaga atgacccatt ttcaatccca acgtaagcaa aaaatacgat
     9301 gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt
     9361 tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt
     9421 agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa caaaaacctt
     9481 gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac
     9541 gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc
     9601 taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat
     9661 gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac
     9721 atcaacatct tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc
     9781 tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc
     9841 acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc
     9901 aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc
     9961 aaaaataacg cgcccattgt taggcatttt ggtgacatca atcacctgcg cgtttgacag
    10021 cttggcttcg atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg
    10081 gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat
    10141 ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg
    10201 taaggtcatc ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt
    10261 cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa
    10321 aaaaataccg acccaggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt
    10381 ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga
    10441 cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt
    10501 gaccagctgt atagcgttca gtgttcaggc cgcaaatgtt gatgagtaca ttactcaact
    10561 ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat
    10621 tgattaccac agtcagcaga tggcgctgcc tgccagtacc cagaaagtga ttactgcgct
    10681 ggcggcgttg attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg
    10741 caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac
    10801 gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca
    10861 aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg
    10921 ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga
    10981 ccgcaactgt ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat
    11041 acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg
    11101 ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga accgctttac
    11161 gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtgcagga
    11221 tggagccagt tatgccggtg caattctgaa agatgagtta aaacaggcgg gtatcacctg
    11281 gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa
    11341 acagtcggcc ccgctgcacg atctgcttaa gattatgctg aaaaagtcgg ataacatgat
    11401 cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtacctg gtacatggcg
    11461 ggccgggtcg gacgctgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa
    11521 caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat
    11581 gatgcaggtg ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct
    11641 gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt
    11701 ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt
    11761 cattaccaca gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt
    11821 agaacctgcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa gccgtttgta
    11881 taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc cggggcttca
    11941 gattattgac aaagtgcgct ttgtttatgc cggatgcggc gtaaacgcct tatccggcct
    12001 acaaaatcgt gcaaattcaa catattgcaa ttctcttgta ggcctgataa gcgtagcgca
    12061 tcaggctgat ttggcgttta tcatcagtga ttaacgcttg taaatgaact caacgccttc
    12121 ttcgtcgtct tcgtcccagt cgtcatccca gtcttcatca tcctcttcag caatctcttc
    12181 aagctgctgg cgatgataat cgtcccacat gaattcgact ttctctggct gtttcgcttc
    12241 ttcagcctgc acgaccgggt tttcaaggat aaaggtcatc acatcccagc agagatcttt
    12301 cacgcccagt ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcttc
    12361 agcaatcgct ttcgctttct cttcagcttc tgccttatcc agcagatcga tcttattgaa
    12421 cactaaccaa cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat
    12481 aatacgcgcg ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa
    12541 caggacgcgg caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc
    12601 agcgccttca atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg
    12661 taccacaccc agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc
    12721 cgccgatacc gcgcgaataa aggtcgattt acccgcgttt ggcatcccca acataccgac
    12781 gtcagccagc agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt
    12841 tttctgccgt ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca
    12901 gccgccctta gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc
    12961 agtaccctgg tcgattacac gcgtacctac cggcacttta atcgtcacgt ctttaccgcg
    13021 cttaccggta caatcgcggc ttgcgccgtt ctgaccgcgc tctgcacgga aagatttttc
    13081 aaaacgataa tcgataagcg tgttcaggtt ttcgtcggct tccatccata cgtcaccacc
    13141 atcaccaccg tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct
    13201 cacgcaacca ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt
    13261 cattttattc tccataaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt
    13321 acgtaatccg ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac
    13381 aaacgcaccg agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc
    13441 cagtgataaa agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct
    13501 cacctgcgct gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt
    13561 attcagtccg caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc
    13621 aggctttgcc agagggaaga gcgcaattgt acataaagtg tacagtaaaa acaggatctg
    13681 cggtgaggcc agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt
    13741 cgccgcaccg accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc
    13801 gaccagactg gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac
    13861 ctgagtgctg cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg
    13921 cgagagttgc ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa
    13981 caggatgaag ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg
    14041 cttacgaaac acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc
    14101 aatactcgcc atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac
    14161 ctgcttcatt gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat
    14221 gccaatgcct gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg
    14281 tcgcaccatg cgcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg
    14341 gctttcatcc gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg
    14401 gtttttcggg cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg
    14461 gtcacgaccg caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat
    14521 gatgctaccc gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc
    14581 tgaatcgcga ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc
    14641 ctcaggtctt aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatgaccc
    14701 tgctgcttac gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga
    14761 ccgtgagcaa caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg
    14821 acttcttcac cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg
    14881 atgtccagct tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca
    14941 ctttggaaaa ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag
    15001 agtgcgctat aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca
    15061 agtgctacag tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg
    15121 ccattttttc agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc
    15181 acagtaaggt aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg
    15241 agttaaccgc gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg
    15301 acgtccaact gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc
    15361 gtccgatgat tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca
    15421 ccattgctgc cctgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg
    15481 atgaatcaga tatgcgcagg ggtaaagcta ccgccaacgc cgcatttggc aatgccgcca
    15541 gcgtgctggt aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt
    15601 cactcaaagt gctggaagtc atgtcagaag ctgtaaacgt catcgcagaa ggtgaagttc
    15661 tgcaactgat gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct
    15721 atagcaaaac cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct
    15781 gtacgccgga ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc
    15841 agttgatcga cgatttactc gattacaatg ccgatggcga acagttaggt aaaaatgtcg
    15901 gcgacgatct gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca
    15961 caccagaaca ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc
    16021 tggaaccggt tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgtg
    16081 ccgaggaaga agcagacaaa gccatcgcag cattacaggt gctcccggac accccttggc
    16141 gagaagcact catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctccct
    16201 tcatcccgcg cagtgcgcgg gatgattcca gtaaattcca taaaaacact aattcagcgc
    16261 tataacacct gaaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcattttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgccg cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggtg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttgg
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttctaagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc aaactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcagaaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcaacaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgaacgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt ccct
//
LOCUS       DAZSBF010000015        35154 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZSBF010000015 DAZSBF010000000
VERSION     DAZSBF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.015"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHXWD_000553"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHXWD_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR7619569.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHXWD_000554"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHXWD_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR7619570.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHXWD_000555"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHXWD_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR7619571.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHXWD_000556"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHXWD_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR7619572.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHXWD_000557"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHXWD_000557"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR7619573.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHXWD_000558"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHXWD_000558"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR7619574.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHXWD_000559"
     CDS             complement(6574..7338)
                     /locus_tag="ACHXWD_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR7619575.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHXWD_000560"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHXWD_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR7619576.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHXWD_000561"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHXWD_000561"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR7619577.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHXWD_000562"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHXWD_000562"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR7619578.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHXWD_000563"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHXWD_000563"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR7619579.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHXWD_000564"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHXWD_000564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR7619580.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHXWD_000565"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHXWD_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR7619581.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHXWD_000566"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHXWD_000566"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR7619582.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHXWD_000567"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHXWD_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR7619583.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHXWD_000568"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHXWD_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR7619584.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHXWD_000569"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHXWD_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR7619585.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHXWD_000570"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHXWD_000570"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR7619586.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALREYVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHXWD_000571"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHXWD_000571"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR7619587.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHXWD_000572"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHXWD_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR7619588.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHXWD_000573"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHXWD_000573"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR7619589.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHXWD_000574"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHXWD_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR7619590.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHXWD_000575"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="ACHXWD_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR7619591.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHXWD_000576"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="ACHXWD_000576"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR7619592.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="ACHXWD_000577"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="ACHXWD_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHXWD_000578"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="ACHXWD_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR7619593.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="ACHXWD_000579"
     CDS             complement(31717..33003)
                     /locus_tag="ACHXWD_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7619594.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="ACHXWD_000580"
     CDS             complement(33054..33995)
                     /locus_tag="ACHXWD_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR7619595.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="ACHXWD_000581"
     CDS             complement(34010..34780)
                     /locus_tag="ACHXWD_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR7619596.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         8424 a         9747 c         9187 g         7796 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tatgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcctg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaataca gcttattgaa tacccattat gagttagcca ttaa
//
LOCUS       DAZSBF010000016        34489 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZSBF010000016 DAZSBF010000000
VERSION     DAZSBF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.016"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            152..304
                     /gene="mokC"
                     /locus_tag="ACHXWD_000582"
     CDS             152..304
                     /gene="mokC"
                     /locus_tag="ACHXWD_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR7619597.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(408..1538)
                     /gene="dnaJ"
                     /locus_tag="ACHXWD_000583"
     CDS             complement(408..1538)
                     /gene="dnaJ"
                     /locus_tag="ACHXWD_000583"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR7619598.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1627..3543)
                     /gene="dnaK"
                     /locus_tag="ACHXWD_000584"
     CDS             complement(1627..3543)
                     /gene="dnaK"
                     /locus_tag="ACHXWD_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR7619599.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3920..4324
                     /locus_tag="ACHXWD_000585"
     CDS             3920..4324
                     /locus_tag="ACHXWD_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR7619600.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4350..5063
                     /gene="msyB"
                     /locus_tag="ACHXWD_000586"
     CDS             4350..5063
                     /gene="msyB"
                     /locus_tag="ACHXWD_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR7619601.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5212..5778
                     /gene="satP"
                     /locus_tag="ACHXWD_000587"
     CDS             5212..5778
                     /gene="satP"
                     /locus_tag="ACHXWD_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR7619602.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5813..6400)
                     /gene="mog"
                     /locus_tag="ACHXWD_000588"
     CDS             complement(5813..6400)
                     /gene="mog"
                     /locus_tag="ACHXWD_000588"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR7619603.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6515..7468)
                     /gene="tal"
                     /locus_tag="ACHXWD_000589"
     CDS             complement(6515..7468)
                     /gene="tal"
                     /locus_tag="ACHXWD_000589"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR7619604.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7747..9176
                     /locus_tag="ACHXWD_000590"
                     /pseudo
     CDS             7747..9176
                     /locus_tag="ACHXWD_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9246..10022
                     /gene="yaaA"
                     /locus_tag="ACHXWD_000591"
     CDS             9246..10022
                     /gene="yaaA"
                     /locus_tag="ACHXWD_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR7619605.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10175..10471)
                     /locus_tag="ACHXWD_000592"
     CDS             complement(10175..10471)
                     /locus_tag="ACHXWD_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR7619606.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10685..11971)
                     /gene="thrC"
                     /locus_tag="ACHXWD_000593"
     CDS             complement(10685..11971)
                     /gene="thrC"
                     /locus_tag="ACHXWD_000593"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR7619607.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11972..12904)
                     /gene="thrB"
                     /locus_tag="ACHXWD_000594"
     CDS             complement(11972..12904)
                     /gene="thrB"
                     /locus_tag="ACHXWD_000594"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR7619608.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12906..15368)
                     /gene="thrA"
                     /locus_tag="ACHXWD_000595"
     CDS             complement(12906..15368)
                     /gene="thrA"
                     /locus_tag="ACHXWD_000595"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR7619609.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15395..15514)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15450..15515)
                     /gene="thrL"
                     /locus_tag="ACHXWD_000596"
     CDS             complement(15450..15515)
                     /gene="thrL"
                     /locus_tag="ACHXWD_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR7619610.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15729..16415)
                     /locus_tag="ACHXWD_000597"
     CDS             complement(15729..16415)
                     /locus_tag="ACHXWD_000597"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR7619611.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16815..16955)
                     /gene="yjjY"
                     /locus_tag="ACHXWD_000598"
     CDS             complement(16815..16955)
                     /gene="yjjY"
                     /locus_tag="ACHXWD_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR7619612.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17051..17767
                     /gene="arcA"
                     /locus_tag="ACHXWD_000599"
     CDS             17051..17767
                     /gene="arcA"
                     /locus_tag="ACHXWD_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR7619613.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17827..19179)
                     /gene="creD"
                     /locus_tag="ACHXWD_000600"
     CDS             complement(17827..19179)
                     /gene="creD"
                     /locus_tag="ACHXWD_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR7619614.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19237..20661)
                     /gene="creC"
                     /locus_tag="ACHXWD_000601"
     CDS             complement(19237..20661)
                     /gene="creC"
                     /locus_tag="ACHXWD_000601"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR7619615.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20661..21350)
                     /gene="creB"
                     /locus_tag="ACHXWD_000602"
     CDS             complement(20661..21350)
                     /gene="creB"
                     /locus_tag="ACHXWD_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR7619616.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21363..21836)
                     /gene="creA"
                     /locus_tag="ACHXWD_000603"
     CDS             complement(21363..21836)
                     /gene="creA"
                     /locus_tag="ACHXWD_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR7619617.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22047..22916
                     /gene="robA"
                     /locus_tag="ACHXWD_000604"
     CDS             22047..22916
                     /gene="robA"
                     /locus_tag="ACHXWD_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR7619618.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22913..23560)
                     /gene="gpmB"
                     /locus_tag="ACHXWD_000605"
     CDS             complement(22913..23560)
                     /gene="gpmB"
                     /locus_tag="ACHXWD_000605"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR7619619.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23612..24133
                     /gene="yjjX"
                     /locus_tag="ACHXWD_000606"
     CDS             23612..24133
                     /gene="yjjX"
                     /locus_tag="ACHXWD_000606"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR7619620.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24218..24544)
                     /gene="trpR"
                     /locus_tag="ACHXWD_000607"
     CDS             complement(24218..24544)
                     /gene="trpR"
                     /locus_tag="ACHXWD_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR7619621.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24634..26571)
                     /gene="sltY"
                     /locus_tag="ACHXWD_000608"
     CDS             complement(24634..26571)
                     /gene="sltY"
                     /locus_tag="ACHXWD_000608"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR7619622.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26782..28449
                     /gene="ettA"
                     /locus_tag="ACHXWD_000609"
     CDS             26782..28449
                     /gene="ettA"
                     /locus_tag="ACHXWD_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR7619623.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28610..28717)
                     /gene="nadS"
                     /locus_tag="ACHXWD_000610"
                     /pseudo
     CDS             complement(28610..28717)
                     /gene="nadS"
                     /locus_tag="ACHXWD_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(28756..29988)
                     /gene="nadR"
                     /locus_tag="ACHXWD_000611"
     CDS             complement(28756..29988)
                     /gene="nadR"
                     /locus_tag="ACHXWD_000611"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR7619624.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30009..31391)
                     /gene="radA"
                     /locus_tag="ACHXWD_000612"
     CDS             complement(30009..31391)
                     /gene="radA"
                     /locus_tag="ACHXWD_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR7619625.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31440..32408)
                     /gene="serB"
                     /locus_tag="ACHXWD_000613"
     CDS             complement(31440..32408)
                     /gene="serB"
                     /locus_tag="ACHXWD_000613"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR7619626.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32514..33158
                     /locus_tag="ACHXWD_000614"
     CDS             32514..33158
                     /locus_tag="ACHXWD_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR7619627.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33186..34202
                     /gene="lplA"
                     /locus_tag="ACHXWD_000615"
     CDS             33186..34202
                     /gene="lplA"
                     /locus_tag="ACHXWD_000615"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR7619628.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34234..34489
                     /locus_tag="ACHXWD_000616"
                     /pseudo
     CDS             34234..34489
                     /locus_tag="ACHXWD_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8133 a         9385 c         8596 g         8375 t
ORIGIN      
        1 cgggtgcttg aggctatctg tctcaggcat tagctgaacg gcagatagag aaaagccccg
       61 agtgatattt tactatcaac ccgaggcccc ctatatgctg aacacatgta gagtgcctct
      121 tactgaccgt aaggtcaagg agaagagagc aatgaagcag cataaggcga tgattgtcgc
      181 cctgatcgtc atctgtatca ccgccgtagt ggcggcgctg gtaacgagaa aagacctctg
      241 tgaggttcac atccgaactg gccagacgga ggttgctgtt ttcacggctt acgaatccga
      301 gtaagagcaa cggcggggag tgatccccgc cacttttcag gtgctcgcat atcttcaacg
      361 caccctaatt ttacccaagc ctgcccacgg gcaggctttt ggggaggtta gcgggtcagg
      421 tcgtcaaaaa acttcttcac accatcaaag aagctctttg agcgcgggct gttgtgctca
      481 ccggttgggc caccgaagct ttcttgcagc tcttgcagca actgtttctg cttctcgttc
      541 aggcctaccg gtgtttcgac gacaacgcgg cacagcaaat caccctgtgc gccaccgcgg
      601 acagacttga cgcctttacc gcgcatacgg aaaagcttac cggtctgggt ttcgccaggc
      661 actttcagtt tgacgcgacc atcaagggtc ggtacttcga tttcgccacc cagcgccgcc
      721 atagcgaagt tgatcgggac ttcgcaatag aggttgttgc cttcacgctc gaaaatcggg
      781 tgctgtttaa cctgaacctg aacgtacaga tcgcctgccg gtgcgccatg ctcgcccgct
      841 tcaccttcgc ccgcaagacg gatgcggtct ccagtgtcca cccctgccgg gattttaacg
      901 gacagcgttt tgctgcgctc aacacgacca tgaccatgac atttgttgca cggatctttg
      961 atcagcgtac cgcggccctg acagtgtgga caggtctgct gtacagcgaa aaatccctgg
     1021 cgcatctgca cctgaccaga accatgacag gtcggacagg tctgcggctg tgtacctggt
     1081 tttgcaccgc taccgtggca aacgtcacac tcttccagag tcggaatgcg gatctctttg
     1141 gtcacgccac gtacagcttc ttcgagggtg agctccatgt tatagcgtaa atcagcaccg
     1201 cgcgccgcac gttgacgacc acgtccgccg ccaaaaatat cgccgaaaac gtcaccaaaa
     1261 atatcgctga agtctgcgcc gccgccaaaa ccgccgccgc ccatgccacc ttgctcaaac
     1321 gcagcatgac catactgatc gtatgccgca cgtttttgcg agtcggtcag aacttcataa
     1381 gcttccttga tctctttaaa tttcgcctcg gcctctttgt caccctggtt acggtccggg
     1441 tggtatttca tggccaggcg tttgtaggcc tttttgattt cacgctcttc cgctgttttg
     1501 gaaacgccta aaatctcgta ataatcttgc ttagccatct tttttaaatt gcccctagat
     1561 gaatgcacgg gcggagagga aattcccctt cgcccgtgtc agtataatta cccgtttata
     1621 gggcgattat tttttgtctt tgacttcttc aaattcagcg tcgacaacat cgtcatcttt
     1681 cgcgttgttt gcagaagcat cagcaccggc agtctgctgc tgggcatgtt gctgctgggc
     1741 gatttccatc agtttctggg aaacctgtgc cagttcctgc attttcgctt cgatagcggc
     1801 tttgtcttca cctttcagag cagtttccag tgcagtcagc gcagactcga tagcagtttt
     1861 gtcgtcagcc ggcagtttgt cgcctgcttc ttcaacctgc ttacgggtgc tgtgcagcag
     1921 atggtcgccc tggttgcgag tctgtaccag ctcttcaaac ttacggtcag cttcggcgtt
     1981 agcttctgcg tcgcgtacca ttttctggat ttcatcttcg ttcagaccag aagaagcctt
     2041 gatggtgatc ttctgctctt taccgctgtt tttatctttc gcggaaacgt gcaagatacc
     2101 gtcagcatcg atatcgaagg taacttcgat ctgcggcatg ccgcgcggtg ccgggttgat
     2161 accatccagg ttgaactgac ccagagattt gttatcagct gcacgtttac gttcaccctg
     2221 cagcacatgg atggttaccg cagactggtt gtcttcagcg gtagagaaca cctggctgtg
     2281 cttggtcggg atagtggtat ttttcgcgat cagcgtcgtc atcacaccgc ccatggtttc
     2341 gatacccaga gacagcgggg taacgtccag cagcagtacg tcttttacgt caccagtcag
     2401 aacaccaccc tgaacagcag caccgattgc tacagcttcg tccgggttaa cgtctttacg
     2461 cggctcttta ccaaagaact cagcaacttt cttctgaacc attggcatac gagtctgacc
     2521 acccacgagg ataacgtcgt cgatatcaga tacggacagg ccagcgtcct gcagtgcaac
     2581 tttcagcggc tcaatggaac ggtttaccag atcttcaacc aggctttcca gtttcgcacg
     2641 agtcactttg atgttcatgt gtttcggacc ggtcgcatct gcagtgatgt acggcaggtt
     2701 aacgtcggtc tgctgagcgg aagacagttc gattttcgct ttttctgccg cttctttcag
     2761 gcgctgcatt gccagcggat cgttgcgcag gtcaatgccc tgatctttct tgaattcttc
     2821 aaccaggtag ttgatcagac ggctgtcgaa gtcttcaccc cccaggtggg tatcaccgtt
     2881 ggtagccaga acttcgaagg ttttttcgcc gtcaacttcg tcgatttcga taatagaaat
     2941 atcgaaagta ccaccaccca ggtcataaac cgcgatagta cggttgccag tacctttgtc
     3001 cagaccgtaa gccagcgcag ctgcggtcgg ttcgttgatg atacgtttta cttccagacc
     3061 agcgatacgg cctgcgtctt tggttgcctg acgctgagca tcgttaaagt atgccggtac
     3121 ggtgataaca gcttcagtta ccggttcacc caagtaatct tcagcggttt tcttcatttt
     3181 tttcagcact tcagcagaaa tctgcggcgg tgccattttc tggcctttaa cttcgaccca
     3241 tgcgtcgccg ttatcagcag caataatttt gaacggcatg atggaaacat cacgctgtac
     3301 ttcttcgtcc tggaagcggc ggccaatcag gcgtttaatc gcaaacaggg tgttttgcgg
     3361 gttcgtcact gcctgacgtt tagccggctg accaaccaga gtttcaccat cctgggtata
     3421 ggcaataata gaaggcgtgg tgcgatcgcc ttcggcgttc tccagcacgc gaggagtggt
     3481 gccatccata atcgctacac aagagttggt agtacccagg tcgataccaa ttattttacc
     3541 catctaaacg tctccactat atattcggtc atcatgtggt tgtgagtctg taataggggc
     3601 gaaacgtctg gtttcaactg cccaatttca tttttttgca gactcactca ctgcggttga
     3661 ctactaaatg gggtcgtaac ccacgtcatc aagggggaga tgcaaaaatt ttttgtgctt
     3721 ttgcgcagaa atttcgcgat atggcaggac aagacgtaaa attgacagca attttattca
     3781 tataaagaat gaatcgcagc caaggcattc atcaaaaaat tgtaataaaa agaaaagatt
     3841 acgtacctga atcttctctt tatcagcagt aaactagtgg gtattcatcc ctctacctct
     3901 tcccactaag agaatcctta tgaaatccgt ttttacgatt tccgccagcc tggcgattag
     3961 cctgatgctg tgctgcacgg cgcaggcaaa cgaccataaa ctcctcggcg tcattgcaat
     4021 gccgcgtaac gaaaccaacg atctggcgct gaaacttcct gtttgtcgca ttgtgaaacg
     4081 catacaactc tctgccgacc atggcgattt acagttaagc ggtgcatcgg tttatttcaa
     4141 agccgcccgt agcgccagtc agagtctgaa tattccttca gaaataaaag aagggcaaac
     4201 cactgactgg atcaacatta acagtgataa cgacaataaa cgctgcgtct caaaaatcac
     4261 cttttccggt catacggtga actcatcgga tatggccacg ctgaaaatta tcggcgacga
     4321 ctaacgccag aatatagaaa ccacaaaaaa tgaatgttaa ttacctgaat gattcagatc
     4381 tggattttct tcagcattgt agtgaggaac agttggcaaa tttcgcccga ttgctcaccc
     4441 ataatgaaaa aggcaaaact cgcctctcca gcgtactgat gcgcaacgaa ctgtttaaat
     4501 cgatggaagg gcatcccgag caacatcgcc gcaactggca gctgattgct ggagaattac
     4561 agcattttgg tggcgatagt atcgccaaca aactgcgcgg acacggtaaa ttgtatcggg
     4621 ccattttgct cgatgtttca aaacgattga agctgaaagc cgacaaagag atgtctacgt
     4681 ttgagattga gcaacagtta ctggaacaat ttctgcgtaa cacctggaag aatatggacg
     4741 aggaacataa gcaggagttt ctgcacgcgg tcgatgccag ggtgaatgag ctggaagagc
     4801 tgctgccgct gctgatgaaa gacaaattat tggcaaaagg cgtgtcgcat ttgctttcca
     4861 gccaattgac ccgcatttta cgcacccacg cagcaatgag cgtgcttggg catggtttgc
     4921 tgcgcggcgc gggtctggga ggccctgtgg gcgcagcatt aaatggggtt aaagcggtca
     4981 gcggcagtgc ctatcgcgtg acaattccag ccgtaataca aatcgcctgc ctgcgccgga
     5041 tggttagcgc cactcaggtc tgacaccgta aaccggggaa aattttcatt ttcccctcaa
     5101 aagatcgtag acactgcccc actggctgat tattatgccg cgccctgaaa acactactgt
     5161 tattcaggga aattatttca ccattcattc gatgatgatt tttgaggaat tatgggcaac
     5221 actaagttgg ctaatccggc accgctgggc ctgatgggct tcggcatgac caccattctg
     5281 cttaacctgc acaacgtggg ttatttcgct ctggacggta ttattcttgc catgggcatt
     5341 ttctacggcg gcatcgcgca aatttttgcc ggtctgctgg agtacaaaaa aggcaacact
     5401 ttcggtttaa ccgcattcac ctcttacggt tctttctggt tgacgctggt tgcgattctg
     5461 ctgatgccga aactgggtct gaccgatgcg ccaaatgcac agttccttgg tgtctacctg
     5521 ggtctgtggg gcgtatttac gctgtttatg ttcttcggca cgctgaaagg cgcacgcgtt
     5581 ctgcaattcg ttttctttag cctgactgtg ctgtttgccc tgctggcgat cggtaacatt
     5641 gccggtaacg ccgcaatcat ccactttgcc ggctggattg gtctgatctg tggtgccagt
     5701 gcaatctatc tggcgatggg tgaagtactg aacgagcagt ttggtcgcac cgttctgccg
     5761 attggtgaat cccactaatt gttttgagat ccccccgctc ggggggattt ttttattcgc
     5821 taacgtcgcg tcttgcactc ttcggtctga atgcagcaac cacttctggt gccgtttcaa
     5881 cgtatggccc ttccagcaac tgaatgcagt acggtacgct ggcaaaaata ccgtgtacca
     5941 caacgttacc cgcagcgtcc ttcacacctt ccagcgtctc tttaatagac ttcggctgac
     6001 cgggtaagtt aaggatcagc gcctgtttgc gaatcacacc aacctgacgc gaaaggatcg
     6061 cagttggtac aaaatgcagg cttatctggc gcatctgttc accaaagcca ggcatctcgc
     6121 ggtccgctac tgccagcgtc gcatcgggcg ttacgtcacg acgcgccggg ccagttccgc
     6181 ccgtggtgag caccagatgg caactcattt catccactag ctcacacaac gtttgctcga
     6241 tgatcgcctg ctcatcgggg attaagcggg tttccagttc aaacggcgtg gttagcgccg
     6301 atgtcagcca ttcttccagc gcagggatgc ctttatcctg ataaacgccg ctggatgcgc
     6361 gatcagagat ggaaactaag ccaatacgta aagtattcat caagattttt ccgtttaaac
     6421 agtctcgtta aacagaatga tacactgcga agggagtgac agacaggctt cagaagaggt
     6481 agcgtgaccg acttcccggt cacgctaaga atgattacag cagatcgccg atcatttttt
     6541 ccagtttttc ctggtcaata gcaaacttac ggataccttc cgccagttta tctactgcca
     6601 ttggatcctg gttgtgctgc cacaggaact cggactcagt gatacgcgcc ggacgcgctt
     6661 tcacttcgcc ggtgtaagac agtttacgtt cgatagcccc ttcgctctca gccagctctt
     6721 tcagcagtgc cggtgcgatg gtaagacggt cgcagcctgc cagttccaga atttcgccga
     6781 tgttacggaa gcttgcgccc ataaccacgg tttcataacc gtgctctttg tagtactggt
     6841 agatttcaga tacagaaacc acgcccggat cttctgccgg agcgtactct ttcttatcag
     6901 tattcgcttt gtaccagtca agaatacggc caacaaatgg cgagatcagg aacacgcctg
     6961 cttccgcaca agcacgagcc tgagcgaagg agaacagcag ggtcaggtta cagttgatgc
     7021 cttctttttc cagctgttct gcagcacgga taccctgcca agtagaagcc agtttgatca
     7081 gaatacgatc gttgctaata ccagcatcgt tgtagagttt gatcaggcgt tttgctttcg
     7141 caattgacgc ttcggtgtca taggaaagac gcgcatcaac ttcagttgag atacggcccg
     7201 gaaccagttt caggatttcc agaccaatat ttactgccag tttgtcggtc gcgtccacga
     7261 tctgctgcgc gcgatcgttg ctctgctgtt tcgcccaggc gacagcatca tcaatcaact
     7321 tacggtattc cggaatctgc gctgcgttaa gaatgagaga agggttggtt gtggcatcct
     7381 gcggttgata cagcttcatt gccgcgatgt ccccagtgtc ggccactacg gtggtgtact
     7441 gacgaaggga ggtcaatttg tccgtcatga tagtatttct ctttaaacag cttgttaggg
     7501 ggatgtaacc ggtctgccct gatgatatca cgacgcaacg tcagcgcaac cgccgacaag
     7561 acggttatcg ccaggcgaga ctgtttcgga tttctgacac tgcgttgata tcgcccgcca
     7621 tttttataca aaacctcatg tatgctacgc agaagttatc aagtacctcg tagcgtatat
     7681 acttcttaaa caattggtaa cgtttacaca ggaaagtcat cgcgaccggc aataagaggg
     7741 atatgcatgc cagatttttt ctcctttatt aacagcgtcc tttggggatc ggtaatgatt
     7801 tacctgctct tcggcgcagg ttgttggttc acttttcgca ccggatttgt gcagtttcgc
     7861 tacatccgcc agtttggcaa aagtcttaaa aatagcattc atccacagcc aggcggttta
     7921 acctcatttc agtcattgtg taccagtctt gcggcgcgcg tgggtagcgg caacctggcc
     7981 ggcgttgcgc tggctattac cgccggtgga cctggagccg tcttttggat gtgggttgcc
     8041 gcgtttatcg gcatggcgac ctcgtttgcc gaatgttccc ttgcacaact ttataaagaa
     8101 cgtgacgtca atggacagtt tcgtggagga ccggcatggt atatggcgcg cgggctgggg
     8161 atgcgttgga tgggcgttct gttcgccgtc tttttgctca tcgcctacgg cataattttc
     8221 agcggagttc aggcgaacgc cgttgcccga gccctgagtt ttcttttgat tttcccccgc
     8281 tggtgacagg cattattctc gctgtctttg ctctgctggc aatcactcgc ggtcttcatg
     8341 gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc gataatctgg gtgctgacca
     8401 gcatggtgat ttgcgtaata aatatcgggc aacttcccca cgtcatttgg tctatttttg
     8461 agagtgcttt tggctggcag gaagcggcag gcggcgcggc gggatatacc ttaagccagg
     8521 cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg ggttcgacgc
     8581 caaacgcggc agcggcagcg gcgtcctggc ctccgcatcc ggcagcgcaa gggattgtcc
     8641 agatgattgg catttttatc gacaccctgg tcatctgtac ggcaagcgcc atgctgatat
     8701 tactggcggg taacgacaca acctacatgc cgctggaagg tattcagctt atccagaagg
     8761 cgatgcgggt gctaatgggt tcctggggtg ctgagtttgt taccctcgtg gttattctgt
     8821 ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc ttctttttac
     8881 gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg caccttcgca acggtcatcg
     8941 gcggcacctt gctaagtctt ccgctgatgt ggcaactggc agatatcata atggcctgca
     9001 tggctattac caatttgacc gccattttac tgctctcgcc tgtggttcat accattgcca
     9061 gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc
     9121 cggatatcgg tcgccagctt tctccggacg cgtgggatga tgtttcgcag gagtaatcac
     9181 aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc ctgcaaggac
     9241 tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc ccgttgacca
     9301 ccacgcgcta tacgctgccg gagctgttag acaattccca gcacttgatc catgaagcgc
     9361 ggaaactgac gcctccgcag attagcacgc tgatgcgcat cagcgacaaa ctggcgggta
     9421 tcaacgccgc tcgctttcat gactggcagc cagatttcac gccggagaat gcccgccagg
     9481 ctattttggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaact ttcagcgaag
     9541 acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat ggcgtactgc
     9601 gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt gagaatgccc
     9661 gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg aacgaggcgc
     9721 tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat tttaaatcgg
     9781 tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat gagaagaacg
     9841 gcaaatttaa gataatcagc ttctacgcta agaaagcacg cggtctgatg agtcgtttca
     9901 ttattgaaaa tcggctgacc aaaccagagc aactgactgg ttttaatagc gaaggttact
     9961 tctttgatga agcttcctcc agcaatggcg aactggtgtt taaacgctac gagcagcgtt
    10021 aagtttgcag tcaatgccgg atgcggcgtg aacgccttat ccggcctaca aaagcatgca
    10081 gattcaatat attgcagaat tcacgtaggc ctgataagac gcgccagcgt cgcatcaggc
    10141 gctgaatgcc ggattgttac ccggcatttg tcatttagcg gtgatgtttg cctggaccat
    10201 gaccgccgtg atgatcatga ggagctttct tatggtggcg cggcggtggc ggcggtccgt
    10261 gtgggtgcca gcgattgcct cgccattcat aatgttgttt ccaccagccg tggtcgcgcc
    10321 agtgacctcc atcccagtaa tagccacgat tatcacgatc gcctatctgt aattttactg
    10381 acgggactaa cgtaatttcc gcagcctgtg ctgccatggg agcgaccaga accagggaaa
    10441 gtgcgagtac gatagattgc atctttttca caggttactc cttttatcat tgcccgttat
    10501 gggtcgatat ccggagatta cgcgatgaga acgacggttg ttattctccg caatcctaat
    10561 ctctaagtta ccgcatttat tgagaatttc tcctttttcc ctgtcgtttt tctccataat
    10621 ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg cctgattgag ataatgaata
    10681 gattttactg atgattcatc atcaatttac gcaacgcagc aaaatcggcg ggcagattat
    10741 gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt tggcagatcc aacgtttcac
    10801 cgagaatcgc ttccacgctc tctttaaatt tcgccggatg cgcggtgccg aggaacaagc
    10861 catattcgcc tggattcaac tggtcacaca gcgcacgata cgctacggca gcgtgcggct
    10921 ccgaggtgta gcccagttct tttaactcac gcattgtctg ttgcgtggtt tcatcatcca
    10981 cggctgcata acccagctct ttcagttgcc agattttgcg gcggaacaac tcttccacac
    11041 gcggccagtt gttcggctga ctcacgtcca tcgcgttgga taacgtcgcc tgagtcgctt
    11101 tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt atcgttcacg ttggtcgcag
    11161 caataaaacg tttcaccggc agaccgagtg acttcgccag cagacccgcc gtcaaatcgc
    11221 cgaagtttcc gcttggcacc gagacaacca gctggttgcg cgcttcctgc ggcagctgcg
    11281 caactgcttc aaagtagtag caaatctgcg ccagcaaacg gctgatgtta atcgagttag
    11341 ccgagtttaa ccccagcgcc actttcagtt cttcatcatc aaacgcctgc ttcaccagcg
    11401 cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc gatattgccg cccaacgtac
    11461 agaacagttt ttcttgcagt ggactgattt tgcctcgtgg atagaggata accactttca
    11521 cattcggcaa accgtagaaa gcatgagcca ctgccgctcc ggtatcaccg gaggtcgcgg
    11581 tcagaatggt cactggctta tcgcccgcaa tatgggtcag catttgtgcc ataaagcgac
    11641 cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa ttccagacaa ccgacatcgc
    11701 tttcaacatt ggcgaccgga gccgggaagg caaacgccgc gcgcacgcgc tcttccagga
    11761 tttcctgcgg gatttcatca ccaataaacg ccgagaggat cttcgcactg cgggtgacaa
    11821 aatccagctt cagcatctca tcaatttcag tcaggctgaa ttccggcagg tcatgcggaa
    11881 aaaacagccc ctgatttttg cccaacccct gggttacggc ttgcgcaaag ctgacctgct
    11941 cgttgtgatc tttcagattg tagagtttca tttagttttc cagtactcgt gcgcccgccg
    12001 tatccagccg gcaaatatga acaaaacctt cctgattttg caggtagttc ttacccaacc
    12061 agtcggcaac gcgctgggcg gtatccggct tgtcacatag agcgaacaag gtcgggccgg
    12121 agccggagat accgctcgct accgcgccga tttccgcgac cgcctgccgc gcctgccgga
    12181 agccaggcag taaccgttca cggtagggtt cagcgataac atctttcatc agcttcgcgg
    12241 caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc agccagatgt cgcccgtgcg
    12301 caatgcaatc ctggcggcga tactgcgccg gtaaaatagc ccgggcttct gccgtcgaga
    12361 ctttaattcc cggatacgcc agcacccaca gccactcatc aaaccctggc acttgctggc
    12421 tgatgatgtc gttttcttcg atcatcaact gcataccacc aagaaaacac ggtgccacgt
    12481 tgtcgtaatg aatgctgccg gagatacgtc cttccagctc gcccatcaaa gccagcaaac
    12541 gggtgtcatt aagtggcttg ccgcagtgtt cattcatcgc catcagcgcc gcgaccaccg
    12601 aacaggcgct ggagcctaag cccgagccga tcggcatatt cttttccaga gtcatcgcca
    12661 ctggaatttg cttgcccagc tccaggcaaa aacgctccca gcactgataa acgatatttt
    12721 cccgtggttc tgacggcagc ttatcggcaa agcgtccgag gttgttgaga ctgaatgtct
    12781 ctgccgcctc aaccgtgact acatctccga gcaatgcacc atcaacgggt gtcaccgccg
    12841 ccccgagcac atcaaacccg acgctcatat tggcactgga agccggggca taaactttaa
    12901 ccatgtcaga ctcctaactt ccatgagagg gtacgtagca gatcggcaaa gacaccagca
    12961 gcggtaacgt cattgcccgc accatatccg cgcagcacca acggcagcgg ctgataatag
    13021 tggctataaa aggccagggc gttttcgcca tttttcactt tgaacagcgg atcattccca
    13081 tccacttcgg caatcttcac gcggcagaca ccatcttcat caatattgcc aacatagcgc
    13141 aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg caaagagatc gtcgagctgt
    13201 gacagattcg ccataaaagc ggcaacatca ccctcggcgt taaactctgc gggcagcaca
    13261 ggttcaattt caatatccgc cagttccagt tcacgtcccg tttcacgagc gagaatcaat
    13321 agtttacgcg ccacatccat accagaaaga tcatctcgcg ggtccggttc ggtataaccc
    13381 atttcccgcg ccagcgtggt cgcctcggag aaactcatgc cttcgtctaa cttgccgaag
    13441 atataagaaa gcgaacctga aagaatgccg gagaacttca ccaattcatc acctgcattg
    13501 agcagatttt gcaggttctc aataaccggt aatccagccc caacgttggt gtcatagagg
    13561 aatttacgcc gcgatttttc cgccgcataa cgcaactgat gatagtaatc catcgacgag
    13621 gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt cgcgcaggaa gtcggcatat
    13681 tgatccgcca ctgcctggct ggaggtgcag tcaacaatga ccgggttcag cagatgatat
    13741 tctttcacga ggcgaattaa gcgcccgaga ttaaacggct ctttggcttg tgcaagttct
    13801 tcctgccagt tttccagatt aaggccatgc acactagtga gcagagcctt cgagttggca
    13861 acgccgcaga cacgtaagtc gatatgttta ttcttcagcc agctttgctg acgcttcagt
    13921 tgctccagca gcgcaccgcc aacgccaccg acgccaatca caaacacttc gataacctga
    13981 tcggtattga acagcatctg atgagtaacg cgcacgccag tggtcgcatc atcgttattt
    14041 accacgacag agattgagcg ttcagaagat ccctgagcaa tggcgacaat gttgatattg
    14101 gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac gcaaggtgcg cataccatca
    14161 cctaccaccg agataatggc cagccgttcc atcaccgcca gcggctccag taagccttct
    14221 ttcagttcca ggtagaactc ttcctgcatt gcccgttcag cccgcacaca gtcgctttgc
    14281 ggaacgcaga aactgatact gtattcggaa gatgattgcg taatcagcac cacggaaata
    14341 cgggcgcgtg acatcgcggc aaagacgcgc gccgccatgc cgaccattcc tttcatcccc
    14401 gggccggaaa cgctgaacat tgccatgtta ttcagattgg aaatgccctt gaccggtaat
    14461 tcgccttcat cacgactggc accaatgagc gtacctggtg cttgaggatt tccggtattt
    14521 ttaatcaggc aagggatctg gaactgggcg atgggggtaa tggtgcgggg gtgaagaact
    14581 ttagcgccga agtaggaaag ctccatcgct tcctggtagg acatcgactt caacaacctc
    14641 gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt caacgtccgt ccaaatctcg
    14701 caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg agtagtcgga accgttgcgt
    14761 ccaagtacca ccagttcgcc tttttcatta ccggcggtga agcctgccat cagcaccatg
    14821 tgatcagccg gaatacgact tgccgcaata cggcgggtgg actcagcaat atcgacggta
    14881 gattcgaggt aatgccccac tgccagtagt ttttcgaccg gatcgataac ggtaacgttg
    14941 tgaccacgcg cttctaacac gccggccata atggcgatcg acattttctc gccacggcaa
    15001 atcagcgcag cgttaatgct atccgggcac tgccctaaca aactaatgcc atgcaggaca
    15061 tgttttattt gggcaaattc ctggtcgacg aaagttttca attgcgccag cgggaatccc
    15121 ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa tacgttcggc atcgctgata
    15181 ttgggtaaag catcctggcc gctaatggtt ttttcaatca tcgccaccag gtggttggtg
    15241 attttggcgg gggcagagag gacggtggcc acctgcccct gcctggcatt gctttccaga
    15301 atatcggcaa cccgcagaaa acgttctgca tttgccactg atgtaccgcc gaacttcaac
    15361 actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa aaagcccgca ctgtcaggtg
    15421 cgggcttttt tctgtgtttc ctgtacgcgt cagcccgcac cgttacctgt ggtaatggtg
    15481 atggtggtgg taatggtggt gctaatgcgt ttcatggatg ttgtgtactc tgtaattttt
    15541 atctgtctgt gcgctatgcc tatattggtt aaagtattta gtgacctaag tcaataaaat
    15601 tttaatttac tcatggcagg taaccagttc agaagctgct atcagacact ctttttttaa
    15661 tccacacaga gacatattgc ccgttgcagt cagaatgaaa agctgaaaaa tacttactaa
    15721 ggcgtttttt atttggtgat atttttttca atatcatgca gcaaacggtg caacattgcc
    15781 gtgtctcgtt gctctaaaag ccccaggcgt tgctgtaacc agtcgaccag ttttatgtca
    15841 tctgccactg ccagagtcgt cagcaatgcc atgactcgtt cgcgtaaagc ttgcagttga
    15901 tgttggtctg ccgttgtgtc acttttcgcc ggttgttgta ttaatgttgc taattgatag
    15961 caatagacca tcaccgcctg ccccagattg agcgaaggat aatccgccac catcggcaca
    16021 ccagtaagaa cgtcagccaa cgctaactct tcgttagtca gcccggaatc ttcgcgacca
    16081 aacaccagcg cggcatggct catccatgaa gatttttcct ctaacagcgg caccagttct
    16141 actggcgtgg cgtagtaatg atatttcgcc cgactgcgcg cagtggtggc gacagtgaaa
    16201 tcgacatcgt gtaacgattc agccagtgtt gggaaaactt taatattatc aataacatca
    16261 ccagatccat gtgcgaccca gcgggtggct ggctccaggt gtgcctgact atcgacaatc
    16321 cgcagttcgc taaaacccat cgttttcatt gcccgcgccg ctgccccaat attttctgct
    16381 ctggcgggtg cgaccaggat aatcgttata cgcatattgc cactcttctt gaccaaataa
    16441 ccgcgaacag ggtgatcact gtcaacttat tacgcggtgc gaatttacaa attcttaacg
    16501 taagtcgcag aaaaagccct ttacttagct taaaaaaggc taaactattt cctgactgta
    16561 ctaacggttt aattgttaaa aaatgctaca tatccttctg tttacttagg ataattttat
    16621 aaaaaataaa tctcgacaat tggattcacc acgtttatta gttgtatgat gcaactagtt
    16681 ggattattaa aataatgtga cgaaagctag catttagata cgatgatttc atcaaactgt
    16741 taacgtgcta caattgaact tgatatatgt caacgaagcg tagttttatt gggtgtccgg
    16801 cccctcttag cctgttatgt tgctgttaaa atggttagga tgacagccgt ttttgacact
    16861 gtcgggtcct gagggaaagt acccacgacc aagctaatga tgttgttgac gttgatggaa
    16921 agtgcatcaa gaacgcaatt acgtacttta gtcatgttac gccgatcatg ttaatttgca
    16981 gcatgcatca ggcaggtcag ggacttttgt acttcctgtt tcgatttagt tggcaattta
    17041 ggtagcaaac atgcagaccc cgcacattct tatcgttgaa gacgagttgg taacacgcaa
    17101 cacgttgaaa agtattttcg aagcggaagg ctatgatgtt ttcgaagcga cagatggcgc
    17161 ggaaatgcat cagatcctct ctgaatatga catcaacctg gtgatcatgg atatcaatct
    17221 gccgggtaag aacggtcttc tgttagcgcg tgaactgcgc gagcaggcga atgttgcgtt
    17281 gatgttcctg actggccgtg acaacgaagt cgataaaatt ctcggcctcg aaatcggtgc
    17341 agatgactac atcaccaaac cgttcaaccc gcgtgaactg acgattcgtg cacgcaacct
    17401 gctgtcccgt accatgaatc tgggtactgt cagcgaagaa cgtcgtagcg ttgaaagcta
    17461 caagttcaat ggttgggaac tggacatcaa cagccgttcg ttgatcggcc ctgatggcga
    17521 gcagtacaag ctgccgcgca gcgagttccg cgccatgctt cacttctgtg aaaacccagg
    17581 caaaattcag tcccgtgctg aactgctgaa gaaaatgacc ggccgtgagc tgaaaccaca
    17641 cgaccgtact gtagacgtga cgatccgccg tattcgtaaa catttcgaat ctacgccgga
    17701 tacgccggaa atcatcgcca ccatccacgg tgaaggttat cgcttctgtg gtgatctgga
    17761 agattaatcg gctttaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt
    17821 tcaaccttat ttccagatac gtaattcatc gtccgttgta acttctttac tggctttcat
    17881 tttcggcagt gaaaacgcat accagtcgat attacgagtc acaaacatca tgccggccag
    17941 cgccaccacc agcacactgg ttcccaacaa cagcgcgcta tcgacagagt tgagcagtcc
    18001 ccacatcact ccatccagca acaacagcgc gagggtaaac aacatgctat tgcgccagcc
    18061 tttcaatacc gcctgcaaat aaataccgtt cattaacgcc ccaatcagac tggcgattat
    18121 ccatgccacg gtaaaaccga tatgttcaga aagcgccagc aagagcaaat aaaacatcac
    18181 caatgaaagc cccaccagca aatattgcat tgggtgtaaa cgttgcgcgg tgagcgtttc
    18241 aaaaacaaag aacgccataa aagtcagtgc aatcagcaga atggcgtact tagtcgcccg
    18301 gtccgttaat tggtattgat cggctggcgt cgtcactgcg acgctaaacg cgaggaagtt
    18361 ttcccagccg gtatcattgc ctgaagcaaa acgctcaccg agattattag caaaccagct
    18421 gctttgccat tgcgcctgaa aacctgactc gctaacttcc cgtttggctg gcaaaaaatc
    18481 acctaaaaaa ctgggatgcg gccagttgct ggttaaggtc atttcgctat tacgcccggc
    18541 aggcaccaca gaaagatcgc cggtaccgct taaattcagg gccatattca gcttcaggtt
    18601 ctgcttccgc cagtcccctt cgggtaaagg gatatgcacg ccctgcccgc cttgctctaa
    18661 cccggtgccg ggttcaatgg tcagcgccgt tccgttaact tcaggcgctt tcaccacacc
    18721 aataccacgc gcatccccga cgctaatcac aataaatggc ttgccaaagg tgatatttgg
    18781 cgcgtcgagt tcgctaagac gcgaaacatc gaaatcggct tttagcgtta aatcactgtg
    18841 ccagacctga ccggtataaa tccctatctt gcgttcttcc acgttctgat tgccatcaac
    18901 catcaatgac tcaggtaacc aaaaatggat aaaacttcgt ttccgctcca cggttttatc
    18961 ctcttcctgt accgtataaa gctcggtcac aggaatagcg ataagcggcc caacaagttt
    19021 ttgcggcccg ctggtacttt ggcgaatcgc atcttccaca tcgctacggt aatcggcacg
    19081 ttcgacaatc acctgccgaa tcagcattat cggaatcaac aacagcaata ctgcaccaaa
    19141 cagggtggtc attttccaga acagggagga tttcaacata gtcttctcct ttgcaatggc
    19201 agcaggatac gaaggctatg tgggaagaat ttgaagctat gtgaagtgac ggtgaagtcg
    19261 aagcgaggcc agcacgccac cttcctgcac gttgcgcagc gtgacttcgc cgttaaacaa
    19321 acgggcgacc tcactgacga acgccaaccc cagaccgctg cttttttgcc catttgcacg
    19381 cggcaaagag taaaagcgtt caaaaatacg tgacagcgcg tagtcaggaa taccactacc
    19441 ggtatccagc accttaagcg tgacgtgttc ctgatccact tcggcgctta gcgttatacg
    19501 accgctctcg ggggtaaaat cgatggcgtt atccagtaaa ttccccagcg cctgatccag
    19561 taacgccggt tcagcagcaa cattaacctc ggtgggcgta acatgtagag tgatgttttt
    19621 ttctgccaac tgcacggtgc gcgcttcgct gacgcggcgg aataatgccg ccacatcaac
    19681 agcagtcaaa acgacttcct gacgattctc cagtcttgcc tggcgtagta acgtttccac
    19741 cagtgcctgc attcgcgcat tttgcgtcag aatgttgtca gtaaaacgag ccactacttc
    19801 aggcggcgga ccttcgcgta aaatttccgc cgcgccacga atcgccgcca gtgggctttt
    19861 tagctcatgg gttaacgcat aaacatactg ctcaatatag tttttccctt ccagcttcac
    19921 gcgcatactt tccagcgcct gcgcgagttt acgcagctcg ctactaccga gatcgggaag
    19981 aggaacgggc ttattgtcag tgacggaatc agcatagcga gtgagccggg caatggagcg
    20041 gttgatccac caaaccatgc ctgcgccaat caccagtgcg atccccaaca aaatggcgct
    20101 ggcccataaa attcgccgct cgctgcgttt aatgaccgga gccatcgccg cgttcggttt
    20161 gcctacgctc aaaacgccaa taagccgcga gccgtccata atcggcgcgg caacatacat
    20221 caccgaactt tcgggatcgg caggattttg caacgtgcta cgcgcaccat actgaccacg
    20281 caacgttagc cagacgtcat tccagcgcga ataatcctgc ccaacggctt tatttgccga
    20341 atcgaacaat actttgccct gcgcatcggt catatagaca tgatattcat tgcgcacttt
    20401 gttaatgcca ccgatattgg cgcgaaacgg gcgatgttgt agctgattaa acgcctgagc
    20461 cagttgccca tgcgttgggt ccccagagag caaatcggga cgcgccagct ccgccagcaa
    20521 cgttgcggta tcgattaacg tcccctccgt tgctcttcgc acgcccggtt taacttcttt
    20581 gacaaaaata gccagtacga accaggccgc caccgccacc agtaaaaaat agcccagcag
    20641 caaccgcatg ccgatacgca ttacaggccc ctcaggctat atcccatgcc gcgatgagta
    20701 ttaatcggag aaagatcggg gttgatggcg cgcagcttgg cacgcaacgt tttaatgtgg
    20761 gtatcgacgg tgcgatcgta ggtgtcctgc gcatcttccc acacgctatc cattagttgc
    20821 tggcgggacc agacgcggcc tggcgacttg agtaacgtct tcagcaataa aaactcatac
    20881 cgcgtcagcg ttaatggcgt gtcaaaccag ctgatctgcg ccgcgggttc attcaattca
    20941 aaatgaccaa tacggatgac gggagacggc gacgagaact tcttcacccg acgcagtaag
    21001 gtgcgcaccc tggcgcacac ttcgcggggt gaaaacggtt tagccacata gtcgtcagcg
    21061 ccaatttcca gcccaagcag gcgatcgacc tcttcacttc gggccgtcag gaacagtaca
    21121 ggtaacgccg gatggagcgc cagtaactgg cggcacaatt caaagccgct gatatcaggc
    21181 aaaccaacat cgagaatcat gacgtcggga acctgctggc gagctttatc cagcaccggc
    21241 aagcctcgct caaagacctc gacggcaaaa ccttcctgct gcaacatgta gaccagcgtg
    21301 tcggctatcc cttgctcatc ttccactaac cagaccgttt cccgttgcat aaatcgcctc
    21361 tgttattgcc gccacggcat gacaggaacc gcgctaatcg cgtttttcgg cgaaccttct
    21421 acaactttgt cggagtaagc cagataagcg agcgcgttgc gtttggcgtc ataaaagcgc
    21481 acgacctgta acgacttaaa gaccagggac gtgcgttttt tgaatactac ctcgccctga
    21541 gctttgccgt ttttaatacg atccgacagt tcaatcggcc cgacttgctg acaagaaata
    21601 gccgcatcgg aggtatcttc cgccagaccc aatccccctt taataccacc tgttttcgcc
    21661 cggctcacat aacaggtgac atttttcaca tcgggatcgt caaaggcttc cacaacaatt
    21721 ttgtgatccg ggccgatcat tttaaatacg gtgtcgaccg aaccaatctc ttctgcatga
    21781 gccaatggcc ccagcattat caggcttaaa gacaggatca aatgcttgta tttcatattg
    21841 ttaccatttt tatctattac acgagcaatt agttcgtcac ggtaaataac atattgatta
    21901 agaacaaaat ctcaatactt ttatttccgt caagccctaa aacatactct actaagaaaa
    21961 aaacactgaa tgctaaaaca gcaaaaaatg ctattatcca attacctgat gtcaggtgct
    22021 cgttgttgaa aggatgagga tattttatgg atcaggccgg cattattcgc gaccttttaa
    22081 tctggctgga aggtcatctg gatcagcccc tgtcgctcga caatgtagcg gcgaaagcag
    22141 gttattccaa gtggcactta cagagaatgt ttaaagatgt cactggccat gctattggcg
    22201 cgtatattcg tgctcgtcgt ttgtcgaaat cggcggtcgc actacgcctg actgcgcgtc
    22261 cgattctgga catcgcgctg caataccgct tcgactctca acagacattt acccgcgcat
    22321 tcaagaagca gtttgcccag actcctgcac tttaccgccg ttctcctgaa tggagcgcct
    22381 ttggtattcg cccgccgcta cgtctgggtg aattcacaat gccagagcac aaatttgtca
    22441 ccctggaaga tacgccgctg attggtgtta cccagagcta ctcctgttcg ctggagcaaa
    22501 tctctgattt ccgccatgaa atgcgttatc agttctggca cgattttctc ggcaacgcgc
    22561 cgaccattcc gccagtgctc tacggcctga atgaaacgcg tccgagtcag gataaagacg
    22621 acgaacaaga ggtattctat accaccgcgt tagcccagga tcaggcagat ggctatgtac
    22681 tgacggggca tccggtgatg ctgcagggcg gcgaatatgt gatgtttacc tatgaaggtc
    22741 tgggaaccgg cgtgcaggag tttatcctga cggtatacgg aacgtgcatg ccaatgctca
    22801 acctgacgcg ccgtaaaggt caggatattg agcgatacta cccggcagaa gacgccaaag
    22861 cgggagatcg cccaattaat ctgcgctgtg aactgctgat tcctatccgc cgttaacgct
    22921 gcagctcatc taatgcaggg gcgtccagat gcgagatgtc ccctgcggtt tcaacgaccc
    22981 agccagacgc cagccacagg ctttcctgat aatccacgcg cgaaatagaa cagttacgca
    23041 gacgtaagcg gcgctctgcc catgctggta atccgagaat cgtactcacc agacatccca
    23101 gtgcaatacc atggctcacc agcagcggtc ggcttccctg cggtaagtcc cggcaagatt
    23161 ccagtgccgc attgacgcga tcgctgagct cctgcattga ctcgccttca ggaatacgcc
    23221 cgtcaacggt gccattgacc agctgccgac gccagttctc ttcttcttcg gtcagagaat
    23281 cgatatgtct tttctccagc acacccatgt ttaattcacg caggcgagaa tcaaagatga
    23341 tgtcacagcc gcaggcctgg gcgatgattt ccgccgtacg ccgggtgcgt cctaaatcgc
    23401 tactgataat atgcgtaatg ccaagctctt tggcacgggt tgccacctgc atcgcctgtt
    23461 gctcaccttt ggcggtcagc gggctgtcag actggccctg aatacgtcgc tcggcgttcc
    23521 actgcgtttc accgtggcgg actaggtata cctgtaacat gctttttttc cgtatactgc
    23581 tgtaacgtta attttctgag taatgctgat tatgcaccaa gttatctgtg cgaccaccaa
    23641 tcccgctaaa attcaggcca ttctgcaggc atttcacgag atcttcggcg aaggatcctg
    23701 ccatattgca tccgttgccg tcgagagcgg tgtaccggaa cagccctttg gcagtgagga
    23761 aacgcgcgct ggcgcacgaa atcgggtagc aaatgcccgc cgtttacttc cagaggctga
    23821 tttttgggtg gcgattgaag ctggcatcga tggtgacagc actttcagtt gggtggtgat
    23881 tgaaaacacc agccagcgcg gcgaggcgcg ttcggctact ttgccgttac ctgcggtgat
    23941 tctgcagaaa gtgcgtgaag gtgaggcgct tggtcccgtg atgtcgcgtt ataccggtat
    24001 tgatgagatt ggtcgtaagg aaggggcgat cggcgtgttt accgccggga aactcactcg
    24061 cgccagcgtc tatcaccagg cggtgatcct ggcgctgagt ccgtttcaca atgccgtgta
    24121 tcaggcattg taggacggat aaggcgttca cgccgcatcc ggcattcggt gcacgatgcc
    24181 tgatgcgacg ctgccgcgtc ttatcaggcc tacaaaatca atcgcttttc agcaacacct
    24241 cttccagcca ctggcgcagc tccacgggcg cggctttcag gctgttagat ccacgcgtaa
    24301 tcgtcgcgat gcccgcgccg agttcatttt ttaactcacg ctgactcatt tcgccacgca
    24361 acagctcttc gacaatacgc acgcgagtcc ccaacgcttc gcgctcatct ggcgtcagca
    24421 tcaggtttaa caacggtaaa tggagatcgt tttggtaggc attcttaagc aggtcgacaa
    24481 aacgtaacca ctcctggtga cgctgttctg ccatcgctgc tgaatagggt gattgttggg
    24541 ccataatatg tcgccattgt tagcggggga agcaaaatgc ctcccccggt tgtactcgct
    24601 aaagagtacg atagcatatc ataaacgtgc ggatcagtaa cgacgtcccc attccgtggc
    24661 gctcatcaac gtcggtttat cccccatgaa ataacggtag taagcgtcat aagccagcac
    24721 gttcttcaca taaccgcgcg tctcggagaa cggaatactc tcgacaaatg ccacagcgtc
    24781 gatacgcccg gcgctgttgc caagccaggt tcgcacccgt ccaggtccgg cgttataagc
    24841 tgctgaggag aaaatacggt tattgccaaa ctgctgataa acatattgca ggtaactggt
    24901 gccaatgttg atattcgttt ccggatccag caattgccca gggctgctat aaccaggaat
    24961 agagaacatc ttcaccgtat gggtcgctgt accaggcata atctgcatca ggccgctggc
    25021 ccctaccggt gatttcactt tcggattcca ggcgctctcc tgacgggcaa tcgccatcgc
    25081 atagctttgc gggatctctt tcccactggt gtaacgtttg aaaagatcgt tgtaagccag
    25141 cgggaatcgc tcttccagat gatcccaaag cttcccggcg atcgttgcct gaacgctaag
    25201 atcccaccat tggttattga aagcataccg agccagttga gcctgctctg tttttgactt
    25261 gctcttcacc agattggccc actcgctacg cgcggtgtta tcgagattcc agtacatcaa
    25321 ctcgcgcacg cgcgccatct ccggcccctg agtcagggcg ctgtcaacat tctgcggcgc
    25381 tttatcaatc ttcagctcat actcttctcc gatgcgttgt gctgcaacca tcgggtagaa
    25441 accacgctgt tgcatgagtt gatgcaaaat ctctttcgct tcagcttcac gtccgcgttc
    25501 cagcaataaa tccacctgcc agtaacgcca ttcatctttc tcttttgctt ccatcggcag
    25561 acgcgccagc caggtattca ggccgcgacg atcgccggtg ccaagtgcca ttcgcacacg
    25621 gcgttcaatc aacgacgtgg attgcgagcg cataatgacg tcatcgcgcc atttcgcctg
    25681 ctcgtcggtg acatcgttgc ccatcaaacg ccaggcgacg atatcgcgca gctcctgaat
    25741 ctgatcttca ttaagctgct gcacctgggc aagcgacggg atcatcagcc gtgcattttc
    25801 agcatcctgc cgcgccacac tggcaaacgc caccgctgcc atttgacggg taaaatcggt
    25861 cgctccggtt gtacgcgcga aggtcagtac cgtattaggg ttgttcgcca gtgaaatgat
    25921 tgccgaggcg atagtctggt aatcggcagg catctgccct gccagcactg ttaccaggcc
    25981 agtgttaccc gctttcatcg ccagacggat acgctctaaa tacgccagcg gatcttgttt
    26041 acctgacgca cgccagacgc taaataactt gtcacaggcg ttaggctggc tcttgccggt
    26101 tagccacagc tctttcgccc cttgccaggc ttcttcactc tgcccggtgt tccatttcgc
    26161 atagtagtaa ttacattgcg cttcggtagt tccgggcttt tccgggctaa aggctaacaa
    26221 gccacgccag tcttcacgcc gcgccagttc attgacgaaa cgagattgca gcgtgcgagc
    26281 gggaggaagc gtggggttag cgcgaacaaa gttagtgacc gtcaccgccg gttgattcat
    26341 cagatcatcg gtgatctggc ggtattccag gtagggataa agcggataat ccttcagtcc
    26401 aggcatcatt tgttcgacca catccatttg tcgattatcc caggcctgct tgatttgcgc
    26461 gtaacggcta cgctgctcat ccagtgagtc ggctcgcgcc acgctgctga ccgtcagcag
    26521 acagacaccg gcagccaaca gccgccaggt aacttgtttg gctttttcca caagcgcatc
    26581 ctctaagtgt aaatacatca atgcagcgtc atgccgcgta atagatttat gctaaccagt
    26641 cattgccgtt tacgccacgt tacggacact tttttacttt tactgcgaag gtgatcgacc
    26701 aactgggatt aggcggggaa aaaggctaca cttcgccttt gaaaactcat catcacgata
    26761 aacatagagg cgaagtccaa cgtggctcaa ttcgtttata ccatgcatcg tgtcggcaaa
    26821 gttgttccgc cgaaacgtca tattttgaaa aacatctctc tgagtttctt ccctggggca
    26881 aaaattggtg tcctgggtct gaacggcgcg ggtaagtcta ccctgctgcg cattatggcg
    26941 ggcattgata aagacatcga aggtgaagcg cgtccgcagc cagacatcaa gattggttac
    27001 ctgccgcagg aaccgcagct gaacccggaa cacaccgtgc gtgagtccat tgaagaagcg
    27061 gtttctgaag tggttaacgc cctgaaacgc ctggatgaag tgtatgcgct gtacgccgat
    27121 ccggatgccg attttgacaa gctggccgct gaacaaggcc gtctggaaga gatcattcag
    27181 gctcacgacg gtcataacct gaacgtacag ctggagcgtg cggcggatgc gctacgtctg
    27241 ccggactggg acgcgaaaat cgctaacctc tccggtggtg agcgtcgtcg cgtagcgttg
    27301 tgccgcctgc tgctggaaaa accagacatg ctgctgctcg acgaaccgac caaccacctg
    27361 gatgccgaat ccgtggcctg gctggaacgc ttcctgcacg acttcgaggg caccgtggtg
    27421 gcgattaccc acgaccgtta cttcctcgat aacgttgcag gctggatcct cgaacttgac
    27481 cgcggtgaag gtattccgtg ggaaggcaac tactcctcct ggctggagca gaaagatcag
    27541 cgcctggcgc aggaagcttc acaagaagcg gcgcgtcgta agtcgatcga gaaagagctg
    27601 gagtgggtac gtcagggaac taaaggccgc cagtcgaaag gtaaagcacg tctggcacgc
    27661 tttgaagagc tgaacagcac cgaatatcag aaacgtaacg aaaccaacga actgtttatt
    27721 ccacctggac cgcgtctggg cgataaagtg ctggaagtca gcaacctgcg taaatcctac
    27781 ggtgatcgcc tgctgattga tgacctgagc ttctcgatcc cgaaaggggc aatcgtcggg
    27841 atcatcggtc cgaacggcgc gggtaaatcg accctgttcc gtatgatctc tggtcaggaa
    27901 cagccggaca gcggcaccat cactttaggt gaaacggtga aactggcgtc ggttgatcag
    27961 ttccgtgact caatggataa cagcaaaacc gtttgggaag aagtttccgg cgggctggat
    28021 attatgaaga tcggcaacac cgagatgcca agccgcgcct acgttggccg ctttaacttt
    28081 aaaggggttg atcagggtaa acgcgttggt gaactttccg gtggtgagcg cggtcgtctg
    28141 catctggcga agctgctgca ggttggcggc aacatgctgc tgctcgacga accaaccaac
    28201 gacctggata tcgaaaccct gcgcgcgctg gaaaacgccc tgctggagtt cccgggctgt
    28261 gcgatggtta tctcgcacga ccgttggttc ctcgaccgta tcgccacgca catcctggac
    28321 taccaggatg aaggtaaagt tgagttcttc gaaggtaact ttactgagta cgaagagtac
    28381 aagaaacgca cgctgggcgc agacgcactg gagccgaagc gtatcaagta caagcgtatt
    28441 gcgaagtaat gcgtaaaatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccaa
    28501 gaaatttcaa tacgttgtga atctccaggc ctgataagac gcggtaagcg tcgcatcagg
    28561 cattgagcac cgaatgccgg atacggcgtg aacgcctttt ccggcctaca acagccttag
    28621 tatccgcaat ttcgtagcgg gtcacccgtg caggtttttt caaaccttgt ttaatctcga
    28681 cagcctcttc cagagaggtt ttcaactcat caaaaaactt attttatacc tcgcctttga
    28741 gccgtttcat cgcggttatc tctgctcccc catcatctcc cgcaccagct ccacgcagcg
    28801 caggaaacgg ctgtcataat cgtcctcttc aacccgcacg aattcgatat tgttctcttc
    28861 cagcatctcc accagcaagt tctggaactc tttgcgatcc accgaactgc cgaggctgcg
    28921 taaaccatcc gccacccacg gcgtgttgtt ctccagcagg atcaccagat cgaaacggta
    28981 ttcatcaatc aacgcctgta cgaacggatg ctcacgccct tcgtactttt tgcagaacgc
    29041 ctgggtagtg acaaaatcag tgtcaataaa cgccacttta ttggcatatt tcactgcaaa
    29101 atcaatgtat tgtgcgtggc ccagcgcgat tttatcgtaa tcggaatact gcaatgcgat
    29161 ctcatcaccg ccgaggtgtg aaaagacata atcgcgacca tattcccacg cactggtggt
    29221 attgaagata ttggcaagtt tgtttaccag ggtggattta ccgctcgact cgccaccaag
    29281 gatcgccacg gtacgtacaa agaacggctt cacttccgta ggaatatatt cccagtagcg
    29341 gaacgggttt tcgcggatct gcgcaccgct gatactcata aaggtacgtt tcggatcgac
    29401 cagcaccgta tcgatcccca gatgttccat atactgtggc gcatcggctt cttccgaggt
    29461 gtagatcagg tccggctgaa tccccttttc agccataaac tttttgatgc cgttgctcca
    29521 cacatcccag ccgtgcggat acggctccat gccctcttcg ttgaaagcat gaatgcgaat
    29581 atttttctga tatttaaaag tttgtaataa ccagcgcaga cgatccggca ccgtgggctg
    29641 ctgcgacatg gcgctgtctt caaacaacgc gcggtcgcgg gtatcgtcaa aacccataat
    29701 gatatgcagc tcgtcaacct ggctacaggc gcgctggata aggtagatat gtccggtatg
    29761 cagcgggtag aacttaccga atacgacacc aatggttttc ttctgtcggg gaaattcaag
    29821 ccccaaaaaa cggtgcaacg cctccagctt ttgcgcgctg gggcttttaa ttttggcatt
    29881 cagtaactgg cttaaatacc ctttggtcat accgctggca tcagctacct gctgtagcgt
    29941 gcagccctgt tgcttgatgg cagttttcag gtaatcaaat gacgacatat ctccctccgt
    30001 atctctcatt ataagtcgtc gaacacgcta agcgcgtcgg agagtttttt aacgccaaaa
    30061 atctgcatcc cttccggcgc ttttttcggt acgttagccg ccggaacaat cgcccggcga
    30121 aaaccgtgtt tcgccgcttc tgaaattcgt tcctgaccgc tgggcaccgg gcggatctcc
    30181 cctgccagcc cgacttcacc aaacaccacc agatcctgtg gcagcggtct gtcacgcagg
    30241 ctggaaacca tcgccagcag taacgctaaa tcggcactgg tttcggttac cttcacgccg
    30301 ccgaccacgt tcacaaatac atcctgatcg gccatttgca gaccaccgtg acggtgcaag
    30361 acagccagca ggattgccag acggttttgt tccagcccca ctgccacgcg gcgcggattc
    30421 gccatcatcg agtgatcgac cagcgcctga atctccacca gcaacggacg cgttccttcc
    30481 cacaccacca tcacggagct gccggaggtc acttcatctc cgcgacttaa gaaaattgcc
    30541 gaagggttgc tgacttcacg cagcccctgt tcggtcatcg cgaagacgcc cagctcattc
    30601 accgcgccga agcggttttt atggctgcgc aaggtgcgaa aacgggagtc ggcatcacca
    30661 tccagaagca ccgaacagtc gatacagtgt tccagcactt tagggccagc cagcgagcca
    30721 tctttggtta cgtgcccaac catgacaatc gccacaccgc gcgttttggc gaagcgcgtc
    30781 agataagccg ccgtttcacg cacctgcgcc acgctaccag gcgatgattg tacatccgcc
    30841 atatgcatca cctggatcga gtcaattacc atcagtttcg gttgctcttc ttcggcaatc
    30901 aggcagatct gttcgatgct ggtttccgac aacatattga gattgtcagt cggcaggcca
    30961 aggcgatgag cgcgcattgc cacctgttgc agcgactctt cgccagtgac atacagcgtt
    31021 ttcatctgct gggccagttt gcacagcgtt tgcagtagca gcgtggattt ccccgcacct
    31081 gggttaccgc caatcagaat ggcgcttccc ggcaccacgc cgccgcctag tacgcggtcg
    31141 aactctttaa atccggtgga aaaacgcggc agctcttcaa ggctgatatc ggagagtttc
    31201 tggacttttg ccaccccggc gctaccggca tagccgctga gacgctcgtt acgcgccacc
    31261 gttggcgacg cagcaagacg cacctcggtg atggtgttcc aggcatgaca ggcactgcac
    31321 tgcccctgcc agcgcggata atcggccccg cattcattac aaacaaaggc gcgttttgga
    31381 gcttttgcca cggtgtacct cgttaatgct gtgccgcccg caggatggcg ggcgagcaat
    31441 tacttctgat tcaggctgcc agagaggatg cagaataccc ccatcaggtc agcgtgacgg
    31501 atggtgactt ccgccttttc attcactttt ggcttggcat ggtaggcaat ccccagccct
    31561 gctgctttga tcatcggcag gtcattagct ccatcgccaa tcgccacggt ctgcgccagc
    31621 gggatttcat actcctgcgc gaggcgagtc aaagttttcg ctttgtactg cgcgtctacg
    31681 atgtcgccaa tcacattgcc ggtaaattta ccgtccatga tctccagttc attggctacc
    31741 acggcggtca ggcgcagctt gtcgcgcaga tattcagcaa agaaagtaaa gccgccggag
    31801 gcaatcgcca ctttccagcc cagcgtttcc agcttgagca ccagttgcgt taagcctggc
    31861 atcagcggca gattttcacg cacctgttgc agaatattgg cgtcagcgcc tttcagcgtc
    31921 gccacacggc tgcgcaggct ggcggtaaaa tcgagttcgc cgcgcatcgc ccgttcggtt
    31981 acttccgcca ccatctcgcc cgttccggcc agtttggcaa tttcatcaat acattcaatc
    32041 tggatggcgg tggaatccat gtccatcacc agcaaacccg gcgtgcgcag gtgcgggatt
    32101 ttccccagcg gggcgacatc cagttgcgct tcatgggcca ggcgcgtagc ccgtgcggtg
    32161 agtgaacctg ccagacgaat cacctgataa tcttccacgc accaggcggc aacaatcacc
    32221 atcgccgcac ccagtttgct ctggtattgg gtcagacgct gtttatccag cccacgacca
    32281 tacagcagcc agccgctacg acctgcgtgg taatccagtg gcatcacttc atcaccactt
    32341 aatgaaagag gcagacccgg ccataaagag acatcttcag gcaggtcgca ccaggtaatg
    32401 ttaggcatta aggctcctgt aaaatcgttc gaagcaggga aaataacgca tgaggctacc
    32461 ttgtatccat tgcttctggc aacattaagt ctcaaatttt caaagggtgg aagatggctc
    32521 gcacaaaact gaaattccgg ctgcatcggg cagtgattgt cctgttctgt cttgccttgt
    32581 tagtggcact gatgcagggg gcgtcatggt ttagtcaaaa ccaccagcga cagcgtaatc
    32641 cacagctgga agaactggcc cgcaccctgg cgcgtcaggt gacgctgaac gttgcaccgc
    32701 tgatgcgtac cgactcacca gatgaaaaac gcattcaggc gatcctcgat cagttaacgg
    32761 atgaaagccg catcctcgac gcgggtgtgt atgacgaaca aggcgatctt atcgcccgtt
    32821 caggcgaaag cgtcgaagtg cgcgaccggc tagcgctcga cggtaaaaaa gcaggcggct
    32881 attttaacca gcagattgtc gagccaattg cgggtaaaaa cggaccgctc ggctatctgc
    32941 gcctgacact cgacacccat acgctcgcca ccgaagccca acaggtggat aacaccacta
    33001 acattttacg cctgatgttg ctgctctcac tggcaatcgg cgtagtgctg acccgcacgc
    33061 tgctacaggg taaacgcacc cgctggcagc aatcgccctt cctgttaacc gccagcaaac
    33121 cggtgccgga agaggaagaa agcgagaaaa aagagtgacc cattactaca agaaaggaaa
    33181 tcgttatgtc cacattacgc ctgctcatct ctgactctta cgacccgtgg tttaacctgg
    33241 cggtggaaga gtgtattttt cgccaaatgc ccgccacgca gcgcgttctg tttctctggc
    33301 gcaatgccga cacggtagtg attggtcgcg cgcagaaccc gtggaaagag tgtaataccc
    33361 ggcggatgga agaagataac gtccgcctgg cgcggcgcag tagcggtggc ggcgcggtgt
    33421 tccacgatct cggcaatacc tgctttacct ttatggctgg caagccggag tacgataaaa
    33481 ccatctctac gtcgattgtg ctcaatgcgc tgaacgcgct cggcgtcagc gccgaagcgt
    33541 ccggacgtaa cgatctggtg gtgaaaacag tcgaaggcga ccgcaaagtc tcaggctcgg
    33601 cctatcgcga aaccaaagat cgtggcttcc accacggcac tttgctgctc aatgccgacc
    33661 ttagccgcct ggcaaactat ctcaatccgg ataaaaagaa actggcggcg aaaggcatta
    33721 cctcagtgcg ttcccgcgtg accaacctca ccgagctgct gccggggatc acccatgagc
    33781 aggtttgcga ggccataacc aaggcctttt tcgcccatta tggcgagcgt gtagaagcgg
    33841 aaatcatctc cccggacaaa acgccagact tgccaaactt cgccgaaacc tttgcccgtc
    33901 agagtagctg ggaatggaac ttcggtcagg ctccggcatt ctcgcatctg ctggatgaac
    33961 gctttagctg gggcggcgtg gaactgcatt tcgacgttga aaaaggccat atcacccgcg
    34021 cccaggtgtt taccgacagc ctcaaccccg cgccgctgga agccctcgcc gggcgactgc
    34081 aaggctgcct gtaccgcgcg gatatgctgc aacaagagtg cgaagcgctg ttggttgact
    34141 tcccggacca ggaaaaagag ctacgggagt tgtcgacgtg gatagcgggg gcggtaaggt
    34201 aactcgctga ttaaaatacc aaaggaaggg aatatgatcg atctggaaaa ccaggagcgg
    34261 gagattatca atttaatgtt aagccagcgt atttcctggc tagccgccgt gcgtatcagg
    34321 cataaattat cacttgctga agtaagtaaa atgttaggta tctcgattaa ttctctaaaa
    34381 tagatcgaaa agacggaacg gcttagctca aacatcaaaa gcaaaatggg agaaatttat
    34441 ggttgtccac cagagttgct gatatgccct tcctggatga cggctgaac
//
LOCUS       DAZSBF010000017        34292 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZSBF010000017 DAZSBF010000000
VERSION     DAZSBF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.017"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHXWD_000617"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHXWD_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR7619629.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHXWD_000618"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHXWD_000618"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR7619630.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHXWD_000619"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHXWD_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR7619631.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHXWD_000620"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHXWD_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR7619632.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHXWD_000621"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHXWD_000621"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR7619633.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHXWD_000622"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHXWD_000622"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR7619634.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHXWD_000623"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHXWD_000623"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR7619635.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHXWD_000624"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHXWD_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR7619636.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHXWD_000625"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHXWD_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR7619637.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHXWD_000626"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHXWD_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR7619638.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHXWD_000627"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHXWD_000627"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR7619639.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHXWD_000628"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHXWD_000628"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR7619640.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHXWD_000629"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHXWD_000629"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR7619641.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHXWD_000630"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHXWD_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR7619642.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHXWD_000631"
     CDS             complement(14282..14581)
                     /locus_tag="ACHXWD_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR7619643.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHXWD_000632"
     CDS             complement(14793..15233)
                     /locus_tag="ACHXWD_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR7619644.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHXWD_000633"
     CDS             15334..15933
                     /locus_tag="ACHXWD_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR7619645.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHXWD_000634"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHXWD_000634"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR7619646.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHXWD_000635"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHXWD_000635"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR7619647.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHXWD_000636"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHXWD_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR7619648.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHXWD_000637"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHXWD_000637"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR7619649.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHXWD_000638"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHXWD_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR7619650.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="ACHXWD_000639"
     CDS             complement(21227..21727)
                     /gene="cpxP"
                     /locus_tag="ACHXWD_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR7619651.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21877..22575
                     /gene="cpxR"
                     /locus_tag="ACHXWD_000640"
     CDS             21877..22575
                     /gene="cpxR"
                     /locus_tag="ACHXWD_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR7619652.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22572..23945
                     /gene="cpxA"
                     /locus_tag="ACHXWD_000641"
     CDS             22572..23945
                     /gene="cpxA"
                     /locus_tag="ACHXWD_000641"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR7619653.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="ACHXWD_000642"
     CDS             complement(24051..24725)
                     /gene="yiiM"
                     /locus_tag="ACHXWD_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR7619654.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="ACHXWD_000643"
     CDS             complement(24874..25857)
                     /gene="kdgT"
                     /locus_tag="ACHXWD_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR7619655.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="ACHXWD_000644"
     CDS             complement(26117..26737)
                     /gene="sodA"
                     /locus_tag="ACHXWD_000644"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR7619656.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27022..28056
                     /gene="rhaT"
                     /locus_tag="ACHXWD_000645"
     CDS             27022..28056
                     /gene="rhaT"
                     /locus_tag="ACHXWD_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR7619657.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="ACHXWD_000646"
                     /pseudo
     CDS             complement(28053..28901)
                     /gene="rhaR"
                     /locus_tag="ACHXWD_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="ACHXWD_000647"
     CDS             complement(28975..29811)
                     /gene="rhaS"
                     /locus_tag="ACHXWD_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR7619658.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30099..31568
                     /gene="rhaB"
                     /locus_tag="ACHXWD_000648"
     CDS             30099..31568
                     /gene="rhaB"
                     /locus_tag="ACHXWD_000648"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR7619659.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31565..32824
                     /gene="rhaA"
                     /locus_tag="ACHXWD_000649"
     CDS             31565..32824
                     /gene="rhaA"
                     /locus_tag="ACHXWD_000649"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR7619660.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33066..33890
                     /gene="rhaD"
                     /locus_tag="ACHXWD_000650"
     CDS             33066..33890
                     /gene="rhaD"
                     /locus_tag="ACHXWD_000650"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR7619661.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33900..34214
                     /gene="rhaM"
                     /locus_tag="ACHXWD_000651"
     CDS             33900..34214
                     /gene="rhaM"
                     /locus_tag="ACHXWD_000651"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR7619662.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8382 a         8940 c         8913 g         8057 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaacacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcgtgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacagcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgccca
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agctaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccacgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactccca ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg
    21181 tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg
    21241 agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca
    21301 actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca
    21361 ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg
    21421 ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc
    21481 gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca
    21541 taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc
    21601 tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt
    21661 cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta
    21721 tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt
    21781 caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc
    21841 tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat
    21901 gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg
    21961 attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt
    22021 ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag
    22081 acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc
    22141 ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg
    22201 gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca aaacaacgac
    22261 aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg tcaggaagcc
    22321 agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg
    22381 gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt gttgggcaaa
    22441 cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg tcgtaaactg
    22501 ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg
    22561 gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc
    22621 tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg
    22681 agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc gaagcggagc
    22741 tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata
    22801 agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg
    22861 ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc
    22921 atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg
    22981 gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca
    23041 ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg gtcagtacgc
    23101 cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg
    23161 ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg
    23221 aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga
    23281 cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc
    23341 tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctcgagcgta
    23401 ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta
    23461 atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg tggagtgaag
    23521 tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc
    23581 cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca
    23641 ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata
    23701 aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg
    23761 aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa tctggcggta
    23821 caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg
    23881 cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga
    23941 gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag
    24001 gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc
    24061 cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc
    24121 gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc gtcatcaaac
    24181 ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc
    24241 aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata caaccacccc
    24301 accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt
    24361 ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca gcggaaaata
    24421 tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc gccaaacgca
    24481 ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag atagtgctca
    24541 cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg
    24601 tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat tttggcaatt
    24661 gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat
    24721 cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg
    24781 cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg acgaacgtta
    24841 gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta aaatttcgat
    24901 tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat
    24961 cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca tcggtttaaa
    25021 cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga
    25081 agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg
    25141 aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac cagtctgggc
    25201 aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa tcagcgtttg
    25261 cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg caaagcccac
    25321 caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt
    25381 gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc
    25441 agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat
    25501 atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa cttcaacacc
    25561 atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt
    25621 taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc
    25681 ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt
    25741 gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa
    25801 tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc
    25861 catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga gcatcagtgt
    25921 tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac cacatcaatt
    25981 gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa
    26041 ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac gcctcattgc
    26101 agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg tcccagttca
    26161 ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc aggtagtaag
    26221 catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa atagcttcac
    26281 ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg cctttcagca
    26341 ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga
    26401 agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc tgcagggtgg
    26461 tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc
    26521 gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg
    26581 gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct
    26641 gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat
    26701 cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt attgtcgggc
    26761 ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa
    26821 aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat
    26881 gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg gcggcaacag
    26941 gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg tgaagcgacg
    27001 aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt ttggcatttg
    27061 atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa aaaatggtca
    27121 tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc gtggaccatc
    27181 agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct ctctacgctg
    27241 ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta tggcctgacc
    27301 atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac gttgattgtc
    27361 ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa caccgaaggc
    27421 ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgtagggat tgtaactcga
    27481 gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa tctgaaaaaa
    27541 gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac
    27601 gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact gtatgtcgct
    27661 ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg tttctgtttt
    27721 attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg
    27781 ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta tctgcaattc
    27841 tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat cagttggatg
    27901 ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg
    27961 aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc
    28021 gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt gagatgacgc
    28081 cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata gttactatct
    28141 tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg gagaagatat
    28201 tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc agtctgctgg
    28261 cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa tttatccagc
    28321 gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag caacgtttcg
    28381 ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa catcaccaac
    28441 tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact actttcatgc
    28501 tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt
    28561 ggttgccctg cgctggcgct aaatcccgga atcgctccct gtcagtcaag attcagcttc
    28621 agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga agcgtaggag
    28681 tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata aggacgatcg
    28741 ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa atcatgtgta
    28801 tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg ctggtcgctg
    28861 gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac ctcggccaga
    28921 gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt cgcgttattg
    28981 cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact ctcggcgaaa
    29041 aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt cagtaacgct
    29101 ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt tcaggtatcg
    29161 ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca gtgaaagaga
    29221 aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct ccagccaggc
    29281 cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg
    29341 cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt
    29401 ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca atacgctgtg
    29461 gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca actgattcag
    29521 cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca gacacagatt
    29581 atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg tgccaccggt
    29641 gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga caatcacaat
    29701 ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg gttctatcgc
    29761 cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca tactggcctc
    29821 ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc ttaaattttc
    29881 gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat
    29941 gcggtgagca tcacatcacc acaatgaagc taattgtgaa catcatcacg ttcatctttc
    30001 cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat tcaggcgctt
    30061 tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc aattgtgtcg
    30121 ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc
    30181 gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc cagaacggtt
    30241 atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta aacaaggtgt
    30301 gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg gactttgtgc
    30361 tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat agccgctcca
    30421 atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat caacgtagcg
    30481 gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg gagcaacaac
    30541 ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc agctatcgcc
    30601 tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg gtcaatatca
    30661 atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa gcctggtttg
    30721 gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag ggtaatgaga
    30781 ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc tcgccgttaa
    30841 acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc ttcgaaagcc
    30901 agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa ggcggggcgg
    30961 aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag cgagtgcttc
    31021 aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca cttccggcct
    31081 gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg atgtgcagcg
    31141 aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat gctgaactgg
    31201 cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc
    31261 agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc cagaacacgc
    31321 tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg cctgttgaag
    31381 cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc aacaatgtgg
    31441 atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc cctaatcctg
    31501 acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca aaggagcttt
    31561 gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt
    31621 ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt caatgcactg
    31681 ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga ccggtgggat
    31741 tcaggctatt ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac gagccgatct
    31801 ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg ccatctatct
    31861 ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca aaaactgggt
    31921 tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt tctcgcatcc
    31981 gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc agttctggat
    32041 tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc
    32101 atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg accgtctcgc
    32161 cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc tggatccggc
    32221 gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga gctacacggt
    32281 tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc tgtgcctgga
    32341 cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg ccatgctgta
    32401 tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg atcacgtggt
    32461 gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg atctgttcga
    32521 ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg ccgcgtgggt
    32581 cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga
    32641 gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac tggaagaaca
    32701 gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg atacgccagc
    32761 aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg
    32821 gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga atgaccatgc
    32881 gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa acatagcctc
    32941 gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg gggagagggt
    33001 tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt acaggaacac
    33061 agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa gccaccaccg
    33121 atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg cgcctggatg
    33181 acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat attcccctca
    33241 gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc ggcaaattct
    33301 tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc gacagcgacg
    33361 gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact tccgaacttc
    33421 cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa gatcgggtga
    33481 tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa aacgacaccg
    33541 cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta ttcccggatg
    33601 gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag gcgaccgcac
    33661 aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc ggcagcgggt
    33721 cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca caagtattag
    33781 tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag ttgatagcgc
    33841 tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa ggagcaaaca
    33901 tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag tatcagcgtc
    33961 ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt gcgcataact
    34021 acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag attgaatctg
    34081 aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg aaatatatga
    34141 ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg caagaagtgt
    34201 tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat tttaaatttc
    34261 gatttgaaaa attaaacata ttacatctcc cg
//
LOCUS       DAZSBF010000018        34111 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZSBF010000018 DAZSBF010000000
VERSION     DAZSBF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.018"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHXWD_000652"
     CDS             complement(<1..716)
                     /locus_tag="ACHXWD_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7619663.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHXWD_000653"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHXWD_000653"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR7619664.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHXWD_000654"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHXWD_000654"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR7619665.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHXWD_000655"
     CDS             complement(3650..4705)
                     /locus_tag="ACHXWD_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR7619666.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHXWD_000656"
     CDS             complement(4715..5503)
                     /locus_tag="ACHXWD_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR7619667.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHXWD_000657"
     CDS             complement(5521..6573)
                     /locus_tag="ACHXWD_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR7619668.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="ACHXWD_000658"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="ACHXWD_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="ACHXWD_000659"
     CDS             complement(7194..8075)
                     /locus_tag="ACHXWD_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR7619669.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="ACHXWD_000660"
     CDS             complement(8096..9406)
                     /locus_tag="ACHXWD_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR7619670.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIVLPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="ACHXWD_000661"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="ACHXWD_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHXWD_000662"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="ACHXWD_000662"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR7619671.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHXWD_000663"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="ACHXWD_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR7619672.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHXWD_000664"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="ACHXWD_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR7619673.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMLDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHXWD_000665"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="ACHXWD_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR7619674.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHXWD_000666"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="ACHXWD_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR7619675.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="ACHXWD_000667"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="ACHXWD_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR7619676.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHXWD_000668"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="ACHXWD_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHXWD_000669"
     CDS             complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="ACHXWD_000669"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR7619677.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHXWD_000670"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="ACHXWD_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHXWD_000671"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="ACHXWD_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR7619678.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHXWD_000672"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="ACHXWD_000672"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR7619679.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /gene="puuA"
                     /locus_tag="ACHXWD_000673"
     CDS             20134..21495
                     /gene="puuA"
                     /locus_tag="ACHXWD_000673"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR7619680.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="ACHXWD_000674"
     CDS             21644..21808
                     /locus_tag="ACHXWD_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619681.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="ACHXWD_000675"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="ACHXWD_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR7619682.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="ACHXWD_000676"
     CDS             23317..23562
                     /locus_tag="ACHXWD_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR7619683.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="ACHXWD_000677"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="ACHXWD_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR7619684.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="ACHXWD_000678"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="ACHXWD_000678"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR7619685.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="ACHXWD_000679"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="ACHXWD_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR7619686.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="ACHXWD_000680"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="ACHXWD_000680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR7619687.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="ACHXWD_000681"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="ACHXWD_000681"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR7619688.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="ACHXWD_000682"
     CDS             29225..29578
                     /locus_tag="ACHXWD_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR7619689.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="ACHXWD_000683"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="ACHXWD_000683"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR7619690.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="ACHXWD_000684"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="ACHXWD_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="ACHXWD_000685"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="ACHXWD_000685"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR7619691.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
BASE COUNT         7824 a         9139 c         8777 g         8371 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc acaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccag
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcgtccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgccgat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg c
//
LOCUS       DAZSBF010000019        34108 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZSBF010000019 DAZSBF010000000
VERSION     DAZSBF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.019"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(244..1164)
                     /gene="malM"
                     /locus_tag="ACHXWD_000686"
     CDS             complement(244..1164)
                     /gene="malM"
                     /locus_tag="ACHXWD_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR7619692.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVSYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1316..2656)
                     /gene="lamB"
                     /locus_tag="ACHXWD_000687"
     CDS             complement(1316..2656)
                     /gene="lamB"
                     /locus_tag="ACHXWD_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR7619693.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2728..3843)
                     /gene="malK"
                     /locus_tag="ACHXWD_000688"
     CDS             complement(2728..3843)
                     /gene="malK"
                     /locus_tag="ACHXWD_000688"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR7619694.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4208..5398
                     /gene="malE"
                     /locus_tag="ACHXWD_000689"
     CDS             4208..5398
                     /gene="malE"
                     /locus_tag="ACHXWD_000689"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR7619695.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5552..7096
                     /gene="malF"
                     /locus_tag="ACHXWD_000690"
     CDS             5552..7096
                     /gene="malF"
                     /locus_tag="ACHXWD_000690"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR7619696.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            7111..8001
                     /gene="malG"
                     /locus_tag="ACHXWD_000691"
     CDS             7111..8001
                     /gene="malG"
                     /locus_tag="ACHXWD_000691"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR7619697.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8373..9848
                     /gene="xylE"
                     /locus_tag="ACHXWD_000692"
     CDS             8373..9848
                     /gene="xylE"
                     /locus_tag="ACHXWD_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR7619698.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(9892..10302)
                     /gene="psiE"
                     /locus_tag="ACHXWD_000693"
     CDS             complement(9892..10302)
                     /gene="psiE"
                     /locus_tag="ACHXWD_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR7619699.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10428..10571)
                     /locus_tag="ACHXWD_000694"
     CDS             complement(10428..10571)
                     /locus_tag="ACHXWD_000694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619700.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            10572..10850
                     /locus_tag="ACHXWD_000695"
     CDS             10572..10850
                     /locus_tag="ACHXWD_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619701.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQSDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(10897..12993)
                     /locus_tag="ACHXWD_000696"
     CDS             complement(10897..12993)
                     /locus_tag="ACHXWD_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR7619702.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(12993..13730)
                     /locus_tag="ACHXWD_000697"
     CDS             complement(12993..13730)
                     /locus_tag="ACHXWD_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR7619703.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13727..14365)
                     /locus_tag="ACHXWD_000698"
     CDS             complement(13727..14365)
                     /locus_tag="ACHXWD_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR7619704.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14479..14721)
                     /gene="yjbE"
                     /locus_tag="ACHXWD_000699"
     CDS             complement(14479..14721)
                     /gene="yjbE"
                     /locus_tag="ACHXWD_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR7619705.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15183..16832)
                     /gene="pgi"
                     /locus_tag="ACHXWD_000700"
     CDS             complement(15183..16832)
                     /gene="pgi"
                     /locus_tag="ACHXWD_000700"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR7619706.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17069..17265
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17357..18706
                     /gene="lysC"
                     /locus_tag="ACHXWD_000701"
     CDS             17357..18706
                     /gene="lysC"
                     /locus_tag="ACHXWD_000701"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR7619707.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            18958..19230
                     /locus_tag="ACHXWD_000702"
     CDS             18958..19230
                     /locus_tag="ACHXWD_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR7619708.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19363..20235)
                     /gene="rluF"
                     /locus_tag="ACHXWD_000703"
     CDS             complement(19363..20235)
                     /gene="rluF"
                     /locus_tag="ACHXWD_000703"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR7619709.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20583..21420
                     /locus_tag="ACHXWD_000704"
                     /pseudo
     CDS             20583..21420
                     /locus_tag="ACHXWD_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            21490..22296
                     /locus_tag="ACHXWD_000705"
     CDS             21490..22296
                     /locus_tag="ACHXWD_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7619710.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22306..22713
                     /locus_tag="ACHXWD_000706"
     CDS             22306..22713
                     /locus_tag="ACHXWD_000706"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR7619711.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            22713..23207
                     /locus_tag="ACHXWD_000707"
                     /pseudo
     CDS             22713..23207
                     /locus_tag="ACHXWD_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23273..24070
                     /locus_tag="ACHXWD_000708"
     CDS             23273..24070
                     /locus_tag="ACHXWD_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR7619712.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24081..24905
                     /locus_tag="ACHXWD_000709"
     CDS             24081..24905
                     /locus_tag="ACHXWD_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR7619713.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            24957..26185
                     /gene="sorE"
                     /locus_tag="ACHXWD_000710"
                     /pseudo
     CDS             24957..26185
                     /gene="sorE"
                     /locus_tag="ACHXWD_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            26568..27258
                     /gene="pepE"
                     /locus_tag="ACHXWD_000711"
                     /pseudo
     CDS             26568..27258
                     /gene="pepE"
                     /locus_tag="ACHXWD_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
     gene            complement(27349..28980)
                     /locus_tag="ACHXWD_000712"
     CDS             complement(27349..28980)
                     /locus_tag="ACHXWD_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR7619714.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29200..32883)
                     /gene="metH"
                     /locus_tag="ACHXWD_000713"
     CDS             complement(29200..32883)
                     /gene="metH"
                     /locus_tag="ACHXWD_000713"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR7619715.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33083..33907
                     /gene="iclR"
                     /locus_tag="ACHXWD_000714"
     CDS             33083..33907
                     /gene="iclR"
                     /locus_tag="ACHXWD_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR7619716.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         8015 a         8927 c         8840 g         8326 t
ORIGIN      
        1 gatatgacct atcataaaac ctctgtaata cagaaataaa ataacctgga aaagtagcct
       61 gagcgtccag acactaccgg cgaaaggaaa agaatctctg ataaggcatt gagataatgg
      121 acattcttag tgaagaagaa gtgaagtact ataaaaaatc gctcatatcc caggaaggaa
      181 gcataaaaaa aggtgcgcca ggagacgcac cagttgttgc aaaatcagca ctgtggggcg
      241 taattacccc ttgcctttta cactgctgat aaaggtggaa cgggcagatg tcgatcccag
      301 gcgttcagct tcatcaagca gttttaacgc cttatcaaca tcacctttcg cgacagcgtt
      361 tttgatcgcg gtattaaaat aactttccgt gtcgttgagc atcggctcgc ttttcttcac
      421 cggcaccgga gcaggtgcag ccacagctgg tgccgccgtg ttgcctaccg taaccggagc
      481 tggagcggaa gaaccaaata gtggtcctac caacacgctg gagctggagt tcgttttgac
      541 tttcagtttc agtaagccat cgctggtatg acgagcaacc ggatcgggaa tatccgggat
      601 cgagttaccg acgcctttgg cataggcttt agccgggtcg agcagttggg tcgtctgctg
      661 gagatctttt tccgtggtaa agaccagaac ataaagtttt tgctgcccca acgccggtgt
      721 caggcgcata acgccttcca gccgatctgc actcatcacg cctggttcct ggtaggtgaa
      781 ataactgctg gggaagaagg ctgatggggt catgttctga tcaagaatca gcacgttcgg
      841 cgcaaaaacg ctggtttgtt tgttcacttc gctggtcagc gtcagggtca gttcgccaat
      901 gtttgccggg acgctgtacg cagcaaccgg accactgatg ccgggaacgt tcagttgttg
      961 gccgccggtc gccagttggg tggtctgggt tttagattga tcgaccggtg tccaggtgag
     1021 ttgttgcagc gcggcagatg gaatggctgg cgcgtcgctg gtgttttgcg gtacgtagct
     1081 aacatcggca aggctaattc caggcgcgct tgccagtaac cctgctgata aacagagggc
     1141 gatgagactt ttattcattt tcattgtttt cacctcaaaa tctggagctc agcggtagcc
     1201 aggcaatagc gcgctaaacc cgataatcag aggggctttc gcccctttca gataatgaca
     1261 acctgttttt atgccggatg cggcgtaaac gccttatccg gcccaggttt tgctattacc
     1321 accagatttc catctgggca ccgaaggtcc actcgtcgct gtcgccacga ccgaagctgc
     1381 cgccgttgaa atcagcagga acggctttgc cgtagttcgg gttaaccttg ctatcgccgt
     1441 tgtagtcgta accccatttc tcatcccact tggcgtaggt tgcgaagaca cgaatagccg
     1501 ggcgtgacca gatgctgtcg ccagcctgcc attgttgtgc gagggtaatt ttgtactgat
     1561 tgttcttgtc gccggtgcgc tgggattcga cgttgtcgta gccgatttcc atcacggtgc
     1621 tcatgattgg cgtccacttg tacatcgggc gaataccgac ggtccaccac ttggtgccgt
     1681 tgtcgttatc ccagttgata tcctggtaca tacccacgta catcatgtcc cagttgtcgc
     1741 ccatggagat cgcaccgtgg tcgaggatac gcagcatgtg accgttgttg ttgatattgt
     1801 aggcaaattt ttcgttatca aacgcgacgc cagaaccctg cgacagacct ttaccctgcg
     1861 aggtcatcga gtcagtagcg tactgaacaa caaacttgtt aaagcccttc aggacactct
     1921 gagtatgttc agcagtgaat aaccagccgt ctttcgatgc gccatcaacc agacgatagt
     1981 tatcacgcag gttggcacga ccgtagtcga cacccagttc taatgtgccg cccgggttga
     2041 tttccatctg cgctaaacgc acatcgaaaa cgtcgttcgc ggtttcgttg gtatagtcat
     2101 aaatattgtt gctggcaaaa gaggaagaac caccagcttc agaggagcgg gttgctgcca
     2161 gagagagttt accgaagcca acatcgatgt tttccagacc ggcaccagga ccagaaatat
     2221 cccagtagta gaagtcgatc atatgaacgt catgacgttg gtagaagcgc ttacctgccc
     2281 agatggtgga acctggcagc cattcgatca ggtttttacc ctgcacgttt gcttcacgga
     2341 aggccggatc ggtagcttcc cagtcattct gttgcgcgac ggaataggcc acgttagtgt
     2401 cgaaatagaa gctcttatcg ccctctttcc acacttcctg acccaatttt aattcagcat
     2461 aagtttcaca ttcgttgcca agacggtatt tactttgagc accggtagtc tggaaacact
     2521 gttgttcacc gccgctacct gtccagccaa taccggaacg tgcatagccg tggaaatcaa
     2581 cagccattgc ctgagcagac attacgcccg ctgcgacggc aaccgccaga ggaagtttgc
     2641 gcagagtaat catcattcta tctcctgagt cattgctttt cttttttcac atcacctgtg
     2701 acaggctttg tgtgttttgt ggggtgctta aacgcccggc tccttatgca gtcgacgaca
     2761 tgcagtgcca tcctcacgga acagatggca acgctctggc ggcaggccga tagcgaatgt
     2821 ggcaccttct tctaccaaca ccacgtcgtt ctggcggtac accaggtttt gacgaatgga
     2881 agggatctgg atatggattt gggtttcgtt gccgagttgc tcgacgacct gaacttcacc
     2941 ctcaaggatg acgtcagcga tatcactcgg cagtagatgt tccgggcgaa tacccagcga
     3001 catattggct ccaacctgga catcacggct ttcaactggc agccagactt gctgacgatt
     3061 tggcatcggc agctccacct gcacttgatc gattgcggtg gcggtcactt ttaccggcag
     3121 gaagttcatc tttggcgaac cgataaatcc ggcgacaaaa cggtctgccg gatagtggta
     3181 cagctccagc ggtttcccaa cctgcgccac gcgaccggcg tccagcacca cgattttgtc
     3241 ggccagcgtc atcgcttcga cctgatcgtg ggtgacgtaa atcattgtgc ggcccaggcg
     3301 tttatgcaga cgggagattt cgatacgcat ttgcacacgc agtgcagcat cgaggttgga
     3361 gagcggttca tcgagcaaaa atacgcttgg ctcggccacc agcgtacggc caatcgccac
     3421 acgctgacgc tgaccaccgg agagcgcttt cggtttgcga tccagcaaat gcgccagttg
     3481 cagcacttcc gccacctggt tgacgcgttg gttaatcacc tctttttttg cgccagccag
     3541 tttcaggcca aatgacatgt tttctgctac tgacaggtgg ggatagagcg cgtaagactg
     3601 aaacaccata ccaacgccgc gttctgctgg cggagtgtca ttcatccgtt tctcaccgat
     3661 gaacaggtcg ccgctggtga tcgtctcaag cccggcaatc atgcgcagta aagtcgattt
     3721 accgcagcca gacggtccga caaacaccac gaattcacct tcatggatat cgagattgat
     3781 atctttcgat accacgacct cgccccaggc tttcgttaca ttttgcagct gtacgctcgc
     3841 catgcccttc tccctttgta acaacctgtc atcgacagca acattcatga tgggctgact
     3901 atgcgtcatc aggagatggc ttaaatcctc caccccctgg cttttttatg ggggaggagg
     3961 cgggaggatg agaacacggc ttctgtgaac taaaccgagg tcatgtaagg aatttcgtga
     4021 tgttgcttgc aaaaatcgtg gcgattttat gtgcgcatct ccacattacc gccaattctg
     4081 taacagagat cacacaaagc aacggtgggg cgtaggggca aggaggatgg aaagaggttg
     4141 ccgtataaag aaactagagt ccgtttaggt gttttcacga gcacttcacc aacaaggacc
     4201 atagattatg aaaataaaaa caggtgcacg catcctcgca ttatccgcat taacgacgat
     4261 gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt aaactggtaa tctggattaa
     4321 cggcgataaa ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg
     4381 aattaaagtc accgttgagc atccggataa actggaagag aaattccctc aggttgcggc
     4441 aactggcgat ggccctgaca ttatcttctg ggcgcacgac cgttttggtg gctacgctca
     4501 atctggcctg ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt
     4561 tacctgggat gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc
     4621 gttatcgctg atttataaca aagatctgct gccgaaccca ccaaaaacct gggaagagat
     4681 cccggcgctg gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca
     4741 agaaccgtac ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga
     4801 aaacggcaag tacgatatta aagacgtggc cgtggataac gctggcgcga aagcgggtct
     4861 gaccttcctg gttgacctta ttaaaaacaa acacatgaat gcagacaccg attactccat
     4921 cgcagaagct gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg
     4981 gtccaacatc gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg
     5041 tcaaccatct aaaccgttcg ttggcgtgct gagcgccggt attaacgccg ccagtccgaa
     5101 caaagagctg gcgaaagagt tcctcgaaaa ctatctgctg actgacgaag gtctggaagc
     5161 ggtcaataaa gacaaaccgc tgggtgcagt ggcgctgaag tcttacgagg aagagttggc
     5221 gaaagatcca cgtattgccg ccaccatgga aaacgcccag aaaggtgaaa tcatgccgaa
     5281 catcccgcag atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag
     5341 cggtcgtcag actgtcgatg aagccctgaa agacgcgcag actcgtatca ccaagtaatg
     5401 ctgtgaaatg ccggatgcgg cgtgaacgcc ttgtccggcc tacaaaactg aaacgcatgt
     5461 aggcctgata agacgcatca gtgtcgcatc aggcagttgt tgtcggataa ggcgtgaaag
     5521 ccttatccgt cctggaatga ggaagaaccc catggatgtc attaaaaaga aacattggtg
     5581 gcaaagcgac gcgctgaaat ggtcagtgct aggtctgctc ggcctgctgg tgggttacct
     5641 tgttgtttta atgtacgcac aaggggaata cctgttcgcc attaccacgc tgatattgag
     5701 ttcagcgggg ctgtatattt tcgccaatcg taaagcctac gcctggcgct atgtttaccc
     5761 gggaatggct ggaatgggat tattcgtcct cttccctctg gtctgcacca tcgccattgc
     5821 cttcaccaac tacagcagca ctaaccagct gacttttgaa tgtgcgcagg aagtgttgtt
     5881 agatcgctcc tggcaagcag gcaaaaccta taactttggt ctttacccgg cgggcgatga
     5941 gtggcaactg gcgctcagtg acggcgaaat cggcaaaaat tacctctccg acgcgtttaa
     6001 atttggcggc gaacaaaaac tgcaactgaa agaaacgacc gcccagcccg aaggcgaacg
     6061 cgcgaatcta cgcgtgatta cccagaatcg tcaggcgctg agtgacatta ccgccattct
     6121 gccggatggc aacaaagtga tgatgagctc cctgcgccag ttttctggca cgcagccgct
     6181 ctacacactc gacggtgacg gcacgttgac gaataatcag agcggcgtga aatatcgtcc
     6241 gaataaccaa attggctttt accagtccat taccgccgac ggcaactggg gtgatgaaaa
     6301 gctaagcccc ggttacaccg tgactactgg ctggaaaaac tttacccgcg tcttcaccga
     6361 cgaaggcatt cagaaaccgt tcctcgccat tttcgtctgg accgtggtgt tctcgctgat
     6421 cactgtcttt ttaacggtgg cggtcggcat ggttctggca tgtctggtgc agtgggaagc
     6481 attgcgcggc aaagcggtct atcgcgttct gctgattctg ccctacgcgg tgccatcatt
     6541 catttcaatc ttgattttca aagggttgtt taaccagagc ttcggtgaaa tcaacatgat
     6601 gttgagcgcg ctgtttggcg tgaagcccgc ctggttcagc gatccgacca ccgcccgcac
     6661 gatgctaatt atcgtcaata cctggctggg ttatccgtac atgatgatcc tctgcatggg
     6721 cttgctgaaa gcgattccgg acgatttgta tgaagcctca gcaatggatg gcgcagatcc
     6781 gttccagaac ttctttaaga ttacgctgcc gctgctgatt aaaccgctga cgccgctgat
     6841 gatcgccagc ttcgccttta actttaacaa cttcgtgctg attcaactgt taaccaacgg
     6901 cggcccggat cgtcttggca cgaccacgcc agccggttat accgacctgc ttgttaacta
     6961 cacctaccgt atcgcttttg aaggcggcgg gggtcaggac ttcggtctgg ctgcagcaat
     7021 tgccacgctg ctcttcctgc tggtgggtgc gctggcgata gtgaacctga aagccacgcg
     7081 aatgaagttt gattaaggga gataacaaaa atggcaatgg tccaaccgaa atcgcaaaaa
     7141 gcacgtttgt ttattactca cctgctactg ctacttttta tcgcggcgat tatgttcccg
     7201 ctgctgatgg tcgtcgctat ctcactgcgc caggggaact ttgcgatcgg cagcctgatc
     7261 ccggagcaaa tctcctggga tcactggaaa ctggctttag gttttagcgt ggaacaggct
     7321 gatggtcgca ttacgccacc gccattcccg gtactgctgt ggctatggaa ctcggtaaag
     7381 gtcgccggga tttccgcgat tggcattgtc gcgctctcta ccacctgcgc ctacgctttc
     7441 gcccgtatgc gctttccagg caaagcgacg ctgctgaaag gaatgctgat tttccagatg
     7501 ttcccggcag tactttcact ggtcgcgttg tatgcgttgt ttgatcgtct gggtgagtac
     7561 attccattca ttggcctgaa tactcacggc ggcgtaattt tcgcgtatct gggtgggatt
     7621 gcgctgcatg tctggaccat caaaggctat ttcgaaacca tcgatagttc gctggaagaa
     7681 gcggctgcgc tggatggtgc gacaccgtgg caggccttcc gccttgtcct gttgccgctg
     7741 tcagtaccga ttctggcggt ggtattcatc ctgtcgttta tcgctgccat tactgaagtt
     7801 ccggtcgcgt cgctgttact gcgtgacgta aacagctaca ccctggccgt ggggatgcag
     7861 caatacctca acccgcaaaa ctacctgtgg ggtgactttg ccgccgctgc cgtgatgtct
     7921 gcattaccga tcaccatcgt cttcttgctg gctcaacgct ggctggtcaa cggcctgacg
     7981 gcaggtggtg tgaaaggtta aagatgttgt tctgccaatg ttatgccgct gcaccctcaa
     8041 cttacgttat cccaacttgt gactgttatt cggcgctcca cggagcgcct ttttttcttt
     8101 cgtctgcaat ctgaatcgtt cgccggttaa tatttccatc atagagctta ttatttttac
     8161 gttatttgtt ttcccactta cgataattct ctttcgtgct ctgagtcacg gcaatagtat
     8221 tgttttcatc aatttttgat aattatcaca attaagatca cagaaaagac attacgtaaa
     8281 cgcattgtaa aaaatgataa ttgccttaac tgcctgacaa ttccaacatc aatgcactga
     8341 taaaagatca gaatggtcta aggcaggtct gaatgaatac ccagtataat tccagttata
     8401 tattttcgat taccttagtc gctacattag gtggtttatt atttggctac gacaccgccg
     8461 ttatttccgg tactgttgag tcactcaata ccgtctttgt tgctccacaa aacttaagtg
     8521 aatccgctgc caactccctg ttaggatttt gcgtagccag cgctctgatt ggttgcatca
     8581 tcggcggtgc cctcggtggt tattgcagta accgcttcgg ccgtcgtgat tcacttaaga
     8641 ttgctgctgt cctgtttttt atttctggtg taggttctgc ctggccagaa cttggtttta
     8701 cctctataaa cccggacaac acagtaccta tttatctggc aggttatgtc ccggaatttg
     8761 ttatttatcg tattattggc ggtattggcg ttggtttagc ctcaatgctt tcaccaatgt
     8821 atattgcgga actggctcca gctcatattc gcggaaaact ggtttcattt aaccagtttg
     8881 cgattatttt cgggcaactt ttagtttact gcgtaaacta ttttattgcc cgttccggtg
     8941 atgccagctg gctgaatact gacggctggc gttatatgtt tgcctcggaa tgtatccctg
     9001 cactgctgtt cttaatgctg ctgtataccg tgccagaaag tcctcgctgg ctgatgtcac
     9061 gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat tatgggcaac acgcttgcaa
     9121 ctcaggcagt acaggaaatt aaacactccc tggatcatgg ccgcaaaacc ggtggtcgtc
     9181 tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat gctctccatc ttccagcaat
     9241 ttgtcggcat caatgtggtg ctgtactacg cgccggaagt gttcaaaacg ctgggggcca
     9301 gcacggatat cgcgctgttg cagaccatta ttgtcggagt tatcaacctc accttcaccg
     9361 ttctggcaat tatgacggtg gataaatttg gtcgtaagcc actgcaaatt atcggcgcac
     9421 tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt ttacactcag gcaccgggta
     9481 ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt tgccatgtcc tggggtccgg
     9541 tatgctgggt accgctgtcg gaaatcttcc cgaatgctat tcgtggtaaa gcgctggcaa
     9601 tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc ctggaccttc ccgatgatgg
     9661 acaaaaactc ctggctggtg gcccatttcc acaacggttt ctcctactgg atttacgggt
     9721 gtatgggcgt tctggcagca ctatttatgt ggaaatttgt cccggaaacc aaaggtaaaa
     9781 cccttgagga gctggaagcc ctctgggaac cggaaacgaa gaaaacacaa caaactgcta
     9841 cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc gtgctttctt tttactcccg
     9901 cttcagccgt ttcgaattac acagccacag agtgatcacc agtaacagga tcgcagccga
     9961 gtagatcaac acatccagtg gcgatttatg atcgacgatg atcaagcgca caatggcggt
    10021 gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa ccggactgaa agtacttcac
    10081 aatcagcgcg ataaattcga aatagagaaa gtaaaccacc agtccttcta ccagctcata
    10141 tttgctggtt tgttctggcg cgaacagcac atctgccaga tgcaccgttt ctttgccgag
    10201 gaagacaacc aaaatcaggc cgaggcacag caggccaaga ttgagtacgg tctgcaaaat
    10261 ggtggagata aactccacgc gcggacgaga gagtgacgtc atagcagtac ctccttcaca
    10321 gtggcgagct tcctgtataa cgcaaaaatg tgacggagat ctatattttt tgtgtatgtt
    10381 ttgttcaacc tggatttgga gcgcgcctcc accaaagccg gatataccta cccgtaggcc
    10441 ggataagccg cgcctggcgg cgcatccggc aagacaaacc gcacccaagc cggtaattca
    10501 aaaatcaccc attcaggtga ttcccagcca ccagcaatgc gcgaaaaatc gacaatgttc
    10561 ctctggaaca aatgaaacgt aacctgataa aggtggtaaa aatgaagcct tattttgctg
    10621 ctttgatgtt atcagtctct gttcttcctg cttatgctgg tccgctcggt actgctgata
    10681 aagccgacct accgcaaagc aatgtctcca gcccaatgat ggcccagtcg ctcaggcaat
    10741 cagacctgca accgatatcg acggacagga agacagaatg ttttcgcctt tacacaccag
    10801 atcgcaaacc gggggtgaac tgcgttcctg atggctcaac aggccattaa gaatgagggt
    10861 gccagtgatt gcgaaaactg gcacctttcc catgacttag cggaaattga cgctacggtc
    10921 gctggtcata tcatacaact ggaacttacg cccaagttgc tgaccaccgt cacgcgtcag
    10981 cggcgtccag ccaatcgccg cacggctgcg tgtcgggcca gacgagaaga gatccaacgg
    11041 taccgagaca tacacgcctt tggtgaagtc cccttcgccg tactcctctt ttgaaacatt
    11101 cgtgatcgtg gcatagccac ccaccaccac gccgctatca aagcgtttgg cgatctccag
    11161 cgtgccgcct ttatcccctg ccagatactg cccgacgctg gctttcacta acacgtcctg
    11221 ggcgaaagat ggcgtccagt aggcggtcag atgtccggtt ttcacgctgt agtcggtgaa
    11281 tttcatcata tcttttgcac tacgccagtc gcgctgttta acgtagttgg catccagacc
    11341 aaacgcccag ttgctgtcca gcggacgata caacacttca gcccccgcac cgccaaacat
    11401 ggtttcgaga taacctccat agacctgacc gtagaagccg ttgcccagat gctggaagta
    11461 gttggcttgc aggttattca cataggcatc gttctgcaca tactcgcgca catgggtacg
    11521 aacgcgcggt aagtgcgagt cctgtggcgg attggtgtag ttaaacttgt cgtagttgtt
    11581 ggcaagattt gcaaacaggc tgccgatggt cagcagatgg tccgtcagcc acaaatctgc
    11641 cgttcccatc acgcccagct gatacatgta aaagttttcc gggccaccga ccgactggtt
    11701 cagcaccgga tcgatatgga aatcaaagcg tgatttgtcg atataccagc cctgctcggt
    11761 ggactgcgga accactggct cgacgcgttt ttgcgccagc gtcgtttcgt ggcccaacgg
    11821 ctctccggcg agatgatttt tcaggctggc gacatcggtt tccgtcgtca cctgcggcat
    11881 gttaaggcga ttttccgtaa tgcggatcgt tttgatccca tccggcagat cgttcatcac
    11941 gatccgatta gcacggatga tcccttcgcg cgaatcacga tatttcacct gctcgccggt
    12001 aacatacagc gtatcgcctt tcgcctggat ctgcggatcg gcaagtccgg cattgtattt
    12061 cagcaacgtt aactgattcg ccaccaccga gtgctgcaaa atggcatcct gcggctgcgg
    12121 ttgatattgc gggcgggcgt tatcgatgta ggacgggcgc agatcgttaa agttggtgcg
    12181 cagcgtgacg ccaaacataa aggtgttgcc acgttcatag ctaaggttaa cgtcggccca
    12241 atcggtaacg cgataaatcg ccccgacgtt aaacttactt ttttgctcca gcttcccggc
    12301 aaaatcctgc tgataattat taccttcata ctccagtttc aggcgcagcg gttgccaggg
    12361 cgtctggtat tccacgccgc caaacagtga ggccgggccg tggaacatct gactgccgtc
    12421 gatagatccc gcttgtttgt agctgttatc gcgatagcaa tatttatcac tggctgaaca
    12481 gagcggattt ttcacattac cgctggtgcc cagatatccc cagcccaggc cgagcgtaaa
    12541 atcgaacggc ccccaggctt tgctggcaac aagatattcc gcatcaaaca gccccgtacc
    12601 gccgatatcc cgcgcaccaa ccgccacttg cggcagccag taactctctt cccacaaacg
    12661 cagtttgaga tcgaaggctt tatctttata cgtttgatcg ccagagaacg cttcgacgct
    12721 gctgtactgc cgggtgcgca cgtcggtgta acgcagcgtt gtttccagcc acgggaagag
    12781 ttgcactgaa gctgagtaat aacggtactg atcgttatcg cgatagttca gactcaactc
    12841 cccttcccgt gccatacgcg cggtgggagt ttgtaataat cctacgccac cgaaatccga
    12901 ctgcgacgga ccaatgggcg caggatatgt ttcgccgtag caagctgtgc taatgcccag
    12961 cgccagtaag ctaagcagat gtcttttttt cattattggg gtatccgctg cgtcagagtc
    13021 tgaagaatgt cggcgttaag ggcatcaggc gtctcactcc agacggagtc tgcgaggcca
    13081 acataaataa tgctgccggg catcggctct acgtgacgct tgttccagta agccaccggc
    13141 gctttttgcg tgcgtccgtc cgggtaaacg acccacgcgt agctgcgatc cgcaccgctg
    13201 agcaggctct ggtcagagag atagctcgcc acgtcgcgac ctggagtgaa tggctgattg
    13261 ccaggatggc tgataagccc gaacaacgtg accgtggacg gcggtggtcc gacccacagc
    13321 gtgtagttgc cctgcaacgg cgggttaccg cgttcggcaa cgcggacgat atcgggatca
    13381 agattgattt tttgcctgcc tgtcacttcc aatgcctgga tttgctggcg taaggcgtta
    13441 atggcggcgg catcgtcggt gctggaatct gcgccctgtt ctgccagccg tgtcagcaac
    13501 gcctgctgct gacgcaatgc tgccgccgtt gccagctctt cgctaatcac cgcaccaggc
    13561 caccagctgt ttgccagtcg aggttgccct accagatcga ttaaatgctc tgcgcccgtt
    13621 agcgttttgg cctcactgct gccattgctg aacaccttga cggttcctgc cgcgaagacc
    13681 gatgacgctc ccacactcaa aagcaacgcg acaatagttt gtttaatcat tgttttgccg
    13741 ccttgatgag agtggttttc accgggaaat aatccgcgcc gagatactgt tccgactggc
    13801 gaatttgccc ttcgctgtcg atccagtagc ggttatgcca gcgcgcctgg tcggtggaga
    13861 cttcttcgtc cagcacgcga accagggttt catcgctgcc gaccttgacg gtatcggtgc
    13921 catcccattt gaagactgag cgtgcggtgg cgtagcgtac ctgctggtac tcggtccagc
    13981 ccatcgtgcg cgtccatgtt gcaccatcaa caatttgtgc gggtttaatc agcgggtcgg
    14041 cggcaaggtt attcacttca atcaggttgt cgccgccaag caaagtcttc accagacgac
    14101 catgttgtgt gacaagagtg gcttgatcct gagtgaccca tttttgttgt ccgtcttcag
    14161 cgaaggccag caccacaaac aattgcggcc cgccattaag ctgcatgtac tggctggcgt
    14221 agggcatatt ttgaatatca tcgtccgtca gctgtacgcc tggcgtgccg aacagactgt
    14281 cccacagtga attgcccagc tctttagtcg tggctgaaca ggcctgtaat aacaggcaga
    14341 taagaatgag tgcaggtcgc ttcacgactc ttctccagag gggcagaata accacaccgg
    14401 agtgtggtta tggttaatcc cttcatattt caagccacag atgcaatgac tcgaattatt
    14461 ttggggatac atactttatt actgggtact ggtggttgtg gttgttgtgg tcccggtatt
    14521 agaaccatca ccgccaccgg ttgccgcgag cgcgacaccc accgccgagc ttacggtgct
    14581 gacgctggtc gcggaggaac tccccgccga aaccgacgtt gctgccgccc ctgccgcttc
    14641 gcccacctgt acaggtgcag cccacacaga agtcgccgca agcgcagata tggcaaaaat
    14701 gccatacaga acttttttca taacaatttc ccttcattga atgaatggag atttacccaa
    14761 aaacatttcg ggcgggatcg agtatacaca actaaagcat gcgaattacg gcgcggggaa
    14821 ttagcgatgg tgttttagga taattggaat gaatatcaaa tataagtttc aacttatatt
    14881 taaataacaa taaaaatcat aaacttaaaa taaaaacaaa agtaattaat ctgcgtattc
    14941 tccgaaataa acaacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt
    15001 taaatcacag acaaggggat ttatctgata aaacaatgcc gacttattgt cggcattgtt
    15061 tttaaggtta tttaactcct catactatgc attaatgtcg atattggcat caggctccgg
    15121 ttgccggatg cggcgtaaac gccttatccg gcctacatat cggcatcagg cacgcatgat
    15181 gattaaccgc gccacgcttt atagcggtta atcagaccat tggtcgagct atcgtggctg
    15241 ctgatttctt tgtcatcttt cagctctggc agaatacggt tcgccagttg tttacccaac
    15301 tccacgcccc actggtcgaa ggtaaagatg ttcaggatca cgccctgagt aaagattttg
    15361 tgctcataca gcgcaatcaa cgcacccagg ctgaacggag tgatttcgcg cagcaggatg
    15421 gagttggtcg ggcggttacc ttcgaacact ttgaacggca ccacgtagtc aagcgttgcc
    15481 ggatctttac cctgatcgcg atattcctgc tcaaccactt cgcgggattt accaaacgcc
    15541 agcgcttcgg tctgggcgaa gaagttagac agcagtttct ggtggtgatc agagagcggg
    15601 ttatgggtga tagccggggc gatgaaatcg cacggtacca ttttggttcc ctggtggatc
    15661 agctggtaga acgcgtgctg accgttagtg cctggttcac cccagataat cgggccagtc
    15721 tggtaatcca caacgttacc gttacggtca acatacttac cgttggactc catattgccc
    15781 tgctggaagt acgccgcgaa acggtgcata tactggtcat acggcagaat cgcttcagtt
    15841 tccgcaccaa agaaattgtt gtaccagatg ccaatcagcg ccagcaggac aggcaggttt
    15901 ttctcggcag gcgtggtgga gaaatgcttg tccatcgcgt gtgcgccaga aagcagttca
    15961 acgaagttat caaagccgat ggagagaaca atcgacaggc caatcgctga ccacaaagag
    16021 taacggccgc cgacccagtc ccagaactcg aacatgttgg cagtatcaat accaaactcg
    16081 ccaacggctt tggcattggt ggaaagcgcc gcaaagtgtt ttgcaacgtg tttttcatca
    16141 cctgccgctt tcaggaacca gtcacgcgcg ctatgggcgt tggtcatagt ttcctgagtg
    16201 gtgaaggttt tagatgctac caggaacagc gtggtttccg ggtttacttt tttcagcact
    16261 tcagcgatgt gagtcccatc gacgttagaa acaaagtgca tgttcaggtg atttttgtac
    16321 ggacgcagtg cttcggtcac catgtacggg ccgaggtcag aaccaccgat cccgatgttc
    16381 actacgtcag tgattgcttt gccggtataa cccttccact caccggagat aatcgcttcc
    16441 gagaaggttt tcatcttctc cagcaccgcg ttgacttccg gcattacgtc tttgccatca
    16501 accagaatcg gggtattgct acggttacgc agcgctacgt gcagcacggc gcggttttca
    16561 gtgcggttga tcttctcgcc agagaacatc gacttaatcg cgcccgccag atcgcactct
    16621 ttcgccagat cctgtaattt cgccagcgtc tcttcagtga tgcggttttt ggagtaatcc
    16681 accagcatct gatcgtcgaa tgttgcggag aacttagaaa aacgatcgcc gtctttagca
    16741 aaaagatcgg cgatcgtaac gtctttcatt tcatcgaagt gtttctgtag tgcctgccag
    16801 gcagcggtct gcgttggatt gatgtttttc attagcaata ctcttctgat tttgagaatt
    16861 gtgactttgg aagattgtag cgccagtcac agaaaaatgt gatggtttta gtgccgttag
    16921 cgtaatgttg agtgtaaacc cttagcgcag tgaagcattt attagctgaa ctactgaccg
    16981 ccaggagtgg atgaaaaatc cgcatgaccc cgtcgttgac aaccgccccg ctcacccttt
    17041 atttataaat gtactacctg cgctagcgca ggccagaaga ggcgcgttgc ccaagtaacg
    17101 gtgttggagg agccagtcct gtgataacac ctgagggggt gcatcgccga ggtgattgaa
    17161 cggctggcca cgttcatcat cggctacagg ggctgaatcc cctgggttgt caccagaagc
    17221 gttcgcagtc gggcgtttcg caagtggtgg agcacttctg ggtgaaaata gtagcgaagt
    17281 atcgctctgc gcccacccgt cttccgctct tcccttgtgc caaggctgaa aatggatccc
    17341 ctgacacgag gtagttatgt ctgaaattgt tgtctccaaa tttggcggta ccagcgtagc
    17401 tgattttgac gccatgaacc gcagcgctga tattgtgctt tctgatgcca acgtgcgttt
    17461 agtagtcctc tcggcttctg ctggtatcac taatctgctg gtcgctttag cggaagggct
    17521 ggaacctggc gagcgattcg aaaaactcga cgctattcgc aacatccagt ttgccattct
    17581 ggaacgtctg cgttacccga acgttatccg tgaagagatt gaacgtctgc tggagaacat
    17641 tactgttctg gcagaagcgg cggcgctggc aacgtctccg gcgctgacag atgagctggt
    17701 cagccacggc gagctgatgt cgaccctgct gtttgtcgaa atcctgcgcg aacgcgatgt
    17761 tcaggcacag tggtttgatg tacgtaaagt gatgcgtacc aacgaccgat ttggtcgtgc
    17821 agagccagat gtagccgcgc tggcggaact ggccgcgctg cagctgctcc cacgcctcaa
    17881 tgaaggctta gtgatcaccc agggatttat cggtagcgaa aataaaggtc gtacaacgac
    17941 gcttggccgt ggaggcagcg attatacggc agccttgctg gcggaggctt tacacgcatc
    18001 tcgtgttgat atctggaccg acgtcccggg catctacacc accgatccac gcgtagtttc
    18061 cgcagcaaaa cgcattgatg aaatcgcgtt tgccgaagcg gcagagatgg caacttttgg
    18121 tgcaaaagta ctgcatccgg caacgttgct acccgcagta cgcagcgata tcccggtctt
    18181 tgtcggctcc agcaaagacc cacgcgcagg tggtacgctg gtgtgcaata aaactgaaaa
    18241 tccgccgctg ttccgcgcgc tggcgcttcg tcgcaatcag actctgctca ctttgcacag
    18301 cctgaatatg ctgcattctc gcggtttcct cgcggaagtt ttcggcatcc tcgcgcggca
    18361 taatatttcg gtagacttaa tcaccacgtc agaagtgagc gtggcattaa cccttgatac
    18421 caccggttca acctccactg gcgatacgtt gctgacgcaa tctctgctga tggagctttc
    18481 cgcactgtgc cgggtggaag tagaagaagg tctggcgctg gtcgcgttga ttggcaatga
    18541 cctgtcaaaa gcctgcggcg ttggcaaaga ggtattcggc gtactggaac cgttcaacat
    18601 tcgcatgatt tgttacggcg catccagcca taacctgtgc ttcctggtgc ccggcgaaga
    18661 tgccgagcag gtggtgcaaa aactgcatag taatttgttt gagtaaatac tgtatggcct
    18721 ggaagctata tttcgggccg tattgatttt cttgtcacta tgcttatcaa taaacgagcc
    18781 tgtactctgt taaccaacgt ctttatcgag aataattgcc tttaattttt ttatctgcat
    18841 ctctaattaa ttatcgaaag agataaatag ttaagagaag gcaaaatgaa tattatcagt
    18901 tctgctcgca aacgaattcc cgtacactga actctctggc tcactgaggg caccatcatg
    18961 gcactccccc gtattaccca aaaagagatg actgaacgcg agcagcgtga acttaaaaca
    19021 ctgctggatc gggcgcggat tgcgcatggt cgcgtactga ccaactccga aaccaacagc
    19081 attaagaaag agtacatcga taaattaatg gtcgagcgtg aggctgaagc gaaaaaagcc
    19141 cgccaattga agaaaaagca ggcttataaa ccggatcctg aagcgtcatt tagctggtca
    19201 gcaaatacgt cgacgcgcgg caggcgctaa tgaacaatca acgcctgaga ttttcactcg
    19261 tcagtgtgaa cacaattttc ccctcaccct aacccttttc ccgaaggggc gaggggactg
    19321 tccgagcact tttgggccgt tttcatcagt ctgaggcgtt aatcagcgcc ccttcttttt
    19381 acgccccggc gaggtaaaac gcttaccgtt ggacgccggg cgaccgcctt tttccgccgt
    19441 tttttccatc ttaacgactg gacgtttgat gcccgctgtt ttcggtttgg ccttcgcttt
    19501 gggcttcgcc tcggaagagg aattttcaat gagcttaaag agatcgataa gctcgtcgtc
    19561 ggttaaatcg cgccattctc ccagcggaat gccgcttaag ctgacgttca taatgcgcgt
    19621 acgttccagc tttttcactt catagccgaa atgctcgcac atgcgccgga tctgacggtt
    19681 caacccctgc accagggtaa tgcggaagac aaacggtgct tcttttttga ctttgcactt
    19741 tttcgtcacc gtcccgagga tcggcactcc cgcccccata ccacgaataa attcatcggt
    19801 aatcggttta tcgaccgtca ccagatactc tttctcatga tcgttacccg cacggaggat
    19861 cttattcacc agatcgccgt gattggtgag gaaaatcagc ccctgggagt ctttatccag
    19921 gcggccaatc gggaacacgc gtttgctgtg gttaacgaaa tcgacaatgt tatcgcgctc
    19981 gccatcttcg gtggtgctga caataccgac gggcttgttc agggcgataa gtaccaaatc
    20041 ttcggcttcc cgaggctcaa tcaactgacc gtttactttc acaatgtcgc cgggtttcac
    20101 ctgatcacca atggtggctc gcttgccatt aaggaacaca ttgccttgct cgatatagcg
    20161 atccgcttcg cggcgtgagc aaattccgct ttcgctgatg tatttattta aacggactga
    20221 tgagtcgggc agcataaatt ctcctgtaaa acgcggaata taccgtacct cttagcgggg
    20281 aaaaacaggg ttatgcggca acgtcgaaat taaatgtgat ctgccacgcc ttttatcaca
    20341 aaagtgtctc cggaagtacg atgcaaattt gcctggcaaa ataaggataa tcaacatatt
    20401 acgaatcgct gcacacctgt gctgatgact actacgcaga gaaacaaatg tgcaagagaa
    20461 agcgctattt ctccccatgc aaagagtgaa aaatcgcttt gaaaggccct cataatgggg
    20521 ttatatcaga tcaagcggtg agaaagcgta tttgcacaaa tgtgcagaac caggaggcgg
    20581 caatggaaaa cagtgacgat atccgtttga ttgtgaagat tgcccaactc tattacgaac
    20641 aggatatgac gcaggcgcaa atcgcgcgcg aactgggtat ttaccgcacc accatcagcc
    20701 gcttgcttaa acgaggccgc gatcagggaa ttgtcaccat cgccatcaac tatgactaca
    20761 acgaaaatct ctggctggag cagcaactga agcaaaagtt tggcttgaaa gacgttgtgg
    20821 tggtgtcggg aaatgatgag gatgaagaga ctcaactggc gatgatgggg ttacacggcg
    20881 cgcaactgct ggatcgcttg ctggagcctg gcgatattgt cggtttttcc tagggtcgcg
    20941 cggtgagcgc actggttgaa aacttgccgc aagcggggca atcgcggcag ttaatctgcg
    21001 tgccaattat tggcggaccg tccggtaaac tcgaaagccg ctatcacgta aacacattaa
    21061 cctacagcgt ggcagcgaag ctgaaagggg aatcgcatct cgcggatttt ccggctctgc
    21121 tggataaccc cttaattcgt aatgggatca tgcagtctca gcactttaaa accatctctg
    21181 cctactggga taatctggat gtcgccctgg tgggaattgg ctcaccggcc attcgcgacg
    21241 gcgctaactg gcatgcgttt tatggtggtg aagagagtga cgacctgaaa gcaggcgcgg
    21301 tattccattg gcattgccat gagtgaagaa aaatacagcg gaattgttgg tgcactgcgt
    21361 ggaaaatata ttaattgtct ggtaacgaat agcagcacag ctgaactatt actgaaataa
    21421 aaatcttccc gatgtttacc gtaataaaaa ttacggcggg tccctattat ctaaaatcag
    21481 gagtctgtta tgcaaacgtg gttaaatttg caggataaaa tcattatcgt caccggcggc
    21541 gcatccggta ttggtctggc gattgtagag gaattattag cacaaggcgc aaacgtacag
    21601 atggtagata ttcacggtgg cgatggtcaa tatgaagttc ataaaggcta tcagttttgg
    21661 ccgaccgata tttccagcgc caaagaggta aatcatacgg tagcggaaat tatccagcgt
    21721 tttggtcgca tcgacggtct ggtcaataac gccggggtca atttcccgcg tctgctggtc
    21781 gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag ctgcattcga aaaaatggtc
    21841 aatatcaacc aaaaaggcgt ttttctgatg tcgcaggcgg tggcgcgcca gatggtcaaa
    21901 caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg gactggaagg ctcagaaggc
    21961 caaagctgtt acgccgctac caaagccgcg ctcaatagct tcacgcgctc ctggtcgaaa
    22021 gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc cggggattct ggaaaaaacc
    22081 gggctacgca cgccggaata tgaagaggcg ctggcgtgga cgcgcaatat caccgtcgag
    22141 cagctgcgtg aaggctatac caaaaacgcc attcctattg ggcgcgccgg aagattagca
    22201 gaagtcgctg attttgtttg ttatctgctg tctgaacgcg ccagctacat caccggagta
    22261 accactaaca ttgcgggcgg caaaacgcgc ggataaggag gctttatggt caacgccatt
    22321 ttttgtgccc acggcaaatt agcttgcgcc atgctggaat cggttcagat ggtgtacggc
    22381 gacgccaatg tcgaggcggt tgaatttgta ccaggcgaga acgccggaga catcgtagca
    22441 aagctggaaa agttagtaag cattcacaat caagatgagt ggctgattgc cgtggattta
    22501 cagtgcggca gcccgtggaa tgcggcagcg atgcttgcga tgcgcaatcc tcgtctacgg
    22561 gtaatcagcg gcctttccct gccgctggcg ctcgaactgg ttgataacca ggacagcatg
    22621 aatgttgatg aactgtgtga acacctgacg caaatcgcaa agcaaacctg cgttgtctgg
    22681 aaacaactgg caacggcaga ggaagatttc tgatgaacat tacgctcgcc cgcattgatg
    22741 accgtctgat ccacggccag gtcactaccg tctggtcgaa agtggctaac gcccagcgca
    22801 ttattatctg caatgacgaa gtttataacg atgaagtgcg ccggacatta ttgcgtcagg
    22861 ctgcgccacc gggaatgaag gttaacgtgg tcaatattga aaaagccgtc gcggtttatc
    22921 acaacccgca atattaggac gaaaccgttt tttatctttt cactcgccca caggatgcct
    22981 tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa tattggcggc atggcctggc
    23041 ggcccggtaa aaaacagtta accaaagcgg tttcattaga tgatgatgat attaacgcat
    23101 ttcatgagtt aaataatctt ggcgtcataa tggatttacg cgtcgtagcg tcagatccct
    23161 caatcaatat tatcgacaaa ataaacgaac agttaatcgc aaattaaata atagcgcctg
    23221 tgtacatatg ctttaacagg cagggatgac gacacttaaa aggtgacaaa tcatggaaat
    23281 aagtacccta caaattattg ctatatttct tttttcctgt attgccggaa tgggcagcgt
    23341 gctggatgaa tttcagactc atcgcccgtt aattgcctgt acggtgattg gtttaattct
    23401 cggtgattta aaaaccggaa taatactcgg tggtacgctg gaattgatag ctctcggctg
    23461 gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc gccagcataa tctccgccat
    23521 tctggttatc gttggtcagc aaagcatcgc caccggaatc gccatcgcgt tgcctgtggc
    23581 tgcggcaggc caagtgctga cggtgtttgc ccgtaccatc accgtagtgt tccagcacgc
    23641 ggcggataaa gcagcggaag aggcgcggtt tcgcactctc gatattctgc atgtctccgc
    23701 gcttggcgtg caggcgctgc gcgttgctat tccggcactg attgtctcac tgttcgtcag
    23761 cgccgatatg gtcagcaata tgttaaacgc cattcccgaa tttgtgaccc gtggactgca
    23821 aattgctggc ggttttatcg tggtggtcgg ttacgccatg gtgctacgta tgatgggcgt
    23881 gaaatatttg atgcctttct ttttcctcgg tttcctcgca ggtggctacc tcgatctcag
    23941 cctgctggcg ttcggtggcg tcggcgtgat catggccctg gtctacatcc agctaaatcc
    24001 acagtggcgt aaagctgaac ctcaactcca ggccaccgcc tccaccgccc ttgaccaact
    24061 tgacgattaa cggagccatc atggaacaga gaaaaattac acgcagcgat ctggtgagca
    24121 tgtttctgcg ctccaacctg caacaggcgt cctttaactt tgaacgtatt cacggactgg
    24181 gcttttgcta cgacatgatc cccgccatca agcgacttta cccattaaaa gaggatcagg
    24241 ttgcggcgct caggcgacac ctggtgttct tcaataccac gccagccgta tgtggcccgg
    24301 tcatcggcgt cactgccgcc atggaagagg cgcgggccaa cggcgcggaa attgatgacg
    24361 gtaccattaa cggcatcaaa gtcggtctga tgggaccatt ggcaggagtt ggcgatccac
    24421 tggtctgggg aacgctgcgc ccgattaccg ctgcgctcgg cgcatctctg gcactttctg
    24481 gcaacatcct cggcccgctg ctgtttttct ttattttcaa tgcggtgcgt ctggcgatga
    24541 agtggtatgg cctacagctc ggctttcgca aaggggtgaa tatcgtcagc gatatgggcg
    24601 ggaatgtgct gcaaaaactc accgaaggcg cgtcgattct cggactgttt gtgatgggcg
    24661 tgctggtcac caaatggacg tcaatcaacg taccgttggt ggtttcacaa acgcatgccg
    24721 ccgatggctc caccgtcatc atgaccgtgc agaacattct cgaccaactt tgccctggtt
    24781 tgctggcgct cggtctgacg ctactgatgg ttcgtctgct caacaaaaaa attaacccgg
    24841 tatggctgat tttcgccctg tttggcttag ggattatcgg caatgcgctg ggcttcctgt
    24901 cctgattctt cgccccggca cgactgccgg ggccatcgct caacatgagg tggtttatga
    24961 aaacaacagc tctgcgtctt tatggtaaac gtgatttacg cctggaaacc tttgaccttc
    25021 ctgaaatgca ggaggatgaa atcctcgcga cggtggtcac tgacagcctg tgcctctctt
    25081 cctggaaaga ggcaacctgg gtgaaaacca taaaaaagta cccgacgatg tggcgaccaa
    25141 cccaatcatc atcggccacg agttttgcgg cgatattctg gccgtgggta aaaagtggca
    25201 gcacaaattc cagccgggtc agcgttatgt gattcaggcc aacctgaaac tccctgaccg
    25261 cccggactgc cccggctact ccttcccgtg ggtgggcggc gaagccacgc atgtggttat
    25321 tcccaacgag gtcatggaac aagattgcct gctggcatac gacggcgaaa cctattttga
    25381 aggctcgctg gttgaaccgc tttcctgcgt gattggcgcg ttcaacgcca actatcatct
    25441 tcaggaaggt agttataacc acacgatggg gattcgcccg caagggcgca cgctgatcct
    25501 cggcggcacc ggaccaatgg gattgttggc gattgattat gcgctgcatg gaccagttaa
    25561 cccgtcgctg ctcgtcatta ccgataccga caacgacaaa ttgagttatg cgcgcaagca
    25621 ctatccatca gaaccgcaaa cactgattca ttatctcaat gccaccgatg cagcatttga
    25681 tacgttaatg gcgctgagtg gcggtcacgg cttcgatgat attttcgtct ttgtgcctaa
    25741 tgaaggactg gtgactctcg cctcttcctt gctggcgaca gatggttgcc tgaatttctt
    25801 cgccggaccg caggataaac atttcagcgc gccaattaat ttctacgatg tgcattatgc
    25861 atttacccac tacgtgggca cgtcaggcgg caataccgac gacatgcgcg cggcggtcaa
    25921 actgattgaa gagaaaaaag tgcaggccgc aaaagtggta acacatattc ttgggctgaa
    25981 tgccgcgggc gaaaccacgc ttgaattgcc tgccgtcggc ggcggcaaaa agctggtgta
    26041 taccgggaaa tacctgccgc tgacgtcact cacgcagatt caggatcaag cactggcggc
    26101 gattctggcg cgtcatcagg ggatctggtc cggtgaggcg gagcaatatc tgctcactca
    26161 tgcagaggca atttcccatg attaatcgcg ctacttaact ctgcatgtcg ctggctggtc
    26221 gccgcggcaa cttcgcgacc tgatgtgcgg ctgattgctg aagcggccca atgtgcacga
    26281 gaaggataaa aaaacatcag cgccttaagg ggcgctcaaa ttggtggcaa agtacacatt
    26341 gtttatgccg gatgcggaat aaagccttat tcggcctaca taatctcgaa aattcaatat
    26401 attgcagtta tctggtaggc ctggtaagcg tagcgcatca ggcaatgctg tgtttgtcat
    26461 cagtctcagc gcccggtgca atacgtgaca tcgtgagcca tgtcagtaag tggttaaatt
    26521 cgcagtggta gcatgccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt
    26581 tattgagtaa ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg
    26641 ctgaacagtt gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga
    26701 cctgtgatga ttacacagcg aaaacggctg cggttctcgc tccgctgggt gtttctgtca
    26761 ccggtattca tagcgttgtc gatcccgttg ccgcgattga aaatgctgag atcgtgattg
    26821 tcggggggcg ggaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggca
    26881 ccaattactg acgtggttaa acgtggtgcg ctgtatattg gctggagcgc aggcgctaac
    26941 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
    27001 gatgcgctaa atctgttccc gctgcaaatc aacccgcact tcaccaacgc gctgccggaa
    27061 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
    27121 ctgacgatta ttggtctacc ggaaggtaac tggatcacgg tgagtaaagg tcacgctacg
    27181 ctgggtggcc cgaacaccac ttatgtgttt aaggccggtg aagaagcggt gccgctggaa
    27241 gcgggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
    27301 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
    27361 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
    27421 gttcagacgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
    27481 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
    27541 aaagcgatgc ttgctgcggc gcaaacgacg agcgctggtg acatcgccag agaagaacac
    27601 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
    27661 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcagcaattt cgctgcccat
    27721 gcgctcgacg atatcggaag cctgttcaag gttgagcgac atttcaataa tctccgccca
    27781 gcgacgcgac tcttcttctg ccagttcctc ttttggcatt cgcgccagat acagcttaat
    27841 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
    27901 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
    27961 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtatccagcg cgctgacatc
    28021 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
    28081 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
    28141 gacgtggaaa tagatcacca gttccgcttt tggcaatgac aacttcccca ttgtctctgc
    28201 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcaaactacc
    28261 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
    28321 ggaaccgagg ttagcaccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
    28381 ggcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
    28441 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
    28501 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
    28561 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
    28621 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
    28681 gagaatacgc gccattagcg ccgtcccgac atctgcaccc agcacaatca ccagagccgg
    28741 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
    28801 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
    28861 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
    28921 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
    28981 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
    29041 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
    29101 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
    29161 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
    29221 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
    29281 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg ggtggctgaa
    29341 ataccaaccc gaaaccgatg caccaggcca catggcgaaa gattctgtga gtttcatgcc
    29401 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
    29461 ggccgggtag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
    29521 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
    29581 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
    29641 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
    29701 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
    29761 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
    29821 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
    29881 caagcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
    29941 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
    30001 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
    30061 cagagtttcg atgctggctt caacttcctg cacgccgaga cggtgcgcca ccggcggcgt
    30121 gtaagcctgc cagtcaaaag cgaagtcgtt atcgcgcgcc gcttccagcg tgaccggtgg
    30181 tgtgcgcggt ttcttgcgcc cgtgctgaat acgtacggtt tcatactcct tgcgggtacg
    30241 agcgacaaaa tcatcgcgct gagtatcgga aagcagcgcc gccaccacac caacggtgcg
    30301 cgaggcattc tgcacataca ccgtcgggcc gctgtagttc tgctcaattt tcaccgccgt
    30361 gtgcgctttt gaggtcgtcg cgccaccgat cagtaacgga atggtgaagc cctgacgctc
    30421 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaagtcc
    30481 aatcagatca gcattcactt ctttagcggt acggagaatt ttttccgcag gcaccataac
    30541 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
    30601 gatgtcgtgg acgtcgccct tcacggtggc gatcaccatc ttgccgttgg ttttgccctg
    30661 ctctttgctg gcttcaataa acggttcgag gtaggccact gcctgtttca tgacgcgcgc
    30721 cgatttgacc acctgtggca ggaacatttt cccctcgcca aacagatcgc cgaccacgtt
    30781 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
    30841 ttcttcggta tcctgctcga taaactcagt aatgcctttg accagcgagt attccagacg
    30901 tttattcact tcccacgagc gccactccgc ctgctgggcg ttagcggtgt cgtcggtttt
    30961 gctgccgcga tatttctcgg caagctccag taaacgttca gtgccatcat cgcgacgatt
    31021 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggc aggtcgtcgt aaatcgccag
    31081 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
    31141 cgcgtgaatg gcttcgcgca ccggttcgtt gccacggaat gagaaagaaa cgttagatac
    31201 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcgcagg cgccgataaa
    31261 gtcctgcgca tagttgttgt gctcttcaat gccggtggcg accgcgaaga tgttcgggtc
    31321 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    31381 gcaaatctcg attttccgtg cgcgggtatc ggcctgtccc tgttcgtcaa aggccattac
    31441 caccaccgct gcaccgtaac gacgcaacag tttcgcatga tggataaagg cgtcgacgcc
    31501 ctctttcatt gagatagagt taacaatgcc tttgccctga atacacttca gccccttttc
    31561 gatgacctcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    31621 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggcg tcgagcatcc cttcatccat
    31681 attgatatcg ataatctgcg cgccgttttc cacctgctgg cgggccacat ccagtgcctc
    31741 gctgtatttc tcttctttga tcaggcgctt gaacttagcg gaaccggtga cgttggtgcg
    31801 ttcacccacg ttcacaaaca ggctatcttc gccaatgttc agcggctcca ggccggacaa
    31861 acggcaggct acggggattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    31921 catcgctgca atatgttgtg gcgtggtgcc acagcagccg ccgacgatat tgagaaagcc
    31981 cgcttccgcc cattcacgta tttgttttgc catcgtgtcg gcgtcgagat cgtactcacc
    32041 aaaggcgttg ggtagcccgg cgttcgggtg cgcggtgacg tagcattccg caatccgtga
    32101 cagctcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    32161 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    32221 gcgcccggag gcgtcggtga tggtgccaga gatcataatc ggcagctcaa cgcccagcgt
    32281 ttcaaactcc gtttttaccg caaataccgc cgctttggcg ttaagggtgt cgaaaacggt
    32341 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggact ctcgataagc
    32401 caccaccagc ccgtcaaaag tgatattacg aaatgccgga tcgttgacgt ccggagaaat
    32461 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    32521 gcgcgcggtc cactcgtcag cacaggcgcg cgccagtttc gccgccgcaa agttgatttc
    32581 cgccgacagg gattccatct ggtaatccgc catcgcaatg gtcgtggagt tgaaggtgtt
    32641 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    32701 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    32761 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    32821 gccgtccagc accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    32881 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    32941 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    33001 catcggatca gttcagcaac tattgcatta actaacaata aaaatgaaaa tgatttccac
    33061 gatacagaaa aaggagactg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    33121 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    33181 actggagtgg attgccgaat ccaatggcag tgtggcactc acggaactgg cgcaacaagc
    33241 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    33301 gcgtcaggtt ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    33361 cagctttctc cagagccgta atttgttagc gattgttcac cctatcctgc gcaatctaat
    33421 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    33481 tattatcgat caggtacagt gtactcacct gatgcgtatg tccgcaccta tcggcggtaa
    33541 attgccgatg cacgcttccg gtgcaggtaa agccttttta gcccaactga gcgaagaaca
    33601 ggtgacgaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa cgctggtgtc
    33661 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggctatt catttgacga
    33721 tgaggaacat gcactggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgtga
    33781 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg accgcgtgac
    33841 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    33901 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    33961 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    34021 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    34081 caagatctca ttccaccgaa acttttac
//
LOCUS       DAZSBF010000020        33999 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZSBF010000020 DAZSBF010000000
VERSION     DAZSBF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33999
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.020"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            12..514
                     /locus_tag="ACHXWD_000715"
                     /pseudo
     CDS             12..514
                     /locus_tag="ACHXWD_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHXWD_000716"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="ACHXWD_000716"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR7619717.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHXWD_000717"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="ACHXWD_000717"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR7619718.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHXWD_000718"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="ACHXWD_000718"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR7619719.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHXWD_000719"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="ACHXWD_000719"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR7619720.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHXWD_000720"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="ACHXWD_000720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR7619721.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHXWD_000721"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="ACHXWD_000721"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR7619722.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHXWD_000722"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="ACHXWD_000722"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR7619723.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHXWD_000723"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="ACHXWD_000723"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR7619724.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHXWD_000724"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="ACHXWD_000724"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR7619725.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHXWD_000725"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="ACHXWD_000725"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR7619726.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHXWD_000726"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="ACHXWD_000726"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR7619727.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHXWD_000727"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="ACHXWD_000727"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR7619728.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHXWD_000728"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="ACHXWD_000728"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR7619729.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHXWD_000729"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="ACHXWD_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR7619730.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18087..19304
                     /gene="alaA"
                     /locus_tag="ACHXWD_000730"
     CDS             18087..19304
                     /gene="alaA"
                     /locus_tag="ACHXWD_000730"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR7619731.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19388..19987
                     /gene="yfbR"
                     /locus_tag="ACHXWD_000731"
     CDS             19388..19987
                     /gene="yfbR"
                     /locus_tag="ACHXWD_000731"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR7619732.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20046..21878)
                     /locus_tag="ACHXWD_000732"
     CDS             complement(20046..21878)
                     /locus_tag="ACHXWD_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR7619733.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="ACHXWD_000733"
                     /pseudo
     CDS             complement(21965..22613)
                     /gene="hxpA"
                     /locus_tag="ACHXWD_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(22624..23118)
                     /locus_tag="ACHXWD_000734"
     CDS             complement(22624..23118)
                     /locus_tag="ACHXWD_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR7619734.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23201..23656)
                     /gene="yfbV"
                     /locus_tag="ACHXWD_000735"
     CDS             complement(23201..23656)
                     /gene="yfbV"
                     /locus_tag="ACHXWD_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR7619735.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            23994..25196
                     /gene="ackA"
                     /locus_tag="ACHXWD_000736"
     CDS             23994..25196
                     /gene="ackA"
                     /locus_tag="ACHXWD_000736"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR7619736.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25271..27415
                     /gene="pta"
                     /locus_tag="ACHXWD_000737"
     CDS             25271..27415
                     /gene="pta"
                     /locus_tag="ACHXWD_000737"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR7619737.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            27605..29125
                     /gene="yfcC"
                     /locus_tag="ACHXWD_000738"
     CDS             27605..29125
                     /gene="yfcC"
                     /locus_tag="ACHXWD_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR7619738.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="ACHXWD_000739"
     CDS             complement(29158..29700)
                     /gene="yfcD"
                     /locus_tag="ACHXWD_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR7619739.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="ACHXWD_000740"
     CDS             complement(29758..30309)
                     /gene="yfcE"
                     /locus_tag="ACHXWD_000740"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR7619740.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="ACHXWD_000741"
     CDS             complement(30365..31009)
                     /gene="yfcF"
                     /locus_tag="ACHXWD_000741"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR7619741.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31145..31792
                     /gene="yfcG"
                     /locus_tag="ACHXWD_000742"
     CDS             31145..31792
                     /gene="yfcG"
                     /locus_tag="ACHXWD_000742"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR7619742.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            31849..32211
                     /gene="folX"
                     /locus_tag="ACHXWD_000743"
     CDS             31849..32211
                     /gene="folX"
                     /locus_tag="ACHXWD_000743"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR7619743.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32232..33125
                     /locus_tag="ACHXWD_000744"
     CDS             32232..33125
                     /locus_tag="ACHXWD_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR7619744.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="ACHXWD_000745"
     CDS             complement(33173..>33999)
                     /gene="rpnB"
                     /locus_tag="ACHXWD_000745"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR7619745.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8481 a         9279 c         8562 g         7677 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gtcaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccaag cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctagtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gttaggcaaa gtaatcttct attattaata
    17641 agcacatcaa aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt
    17701 aattccgctt aacaatagtt tcacaccttg cccccattgc gacacctcgg aaatcatcac
    17761 gctgttaaac tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa
    17821 atgaggcatt ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatc
    17881 agaacaattc actatataac attgcatgca aagcatatac gcctcattat tttgtcatta
    17941 ttaagttatt aacagcacaa tcgagccttc ccctctggca aaatcttatt ctgcagacct
    18001 tcaaaacacc gtcctggggg agtacattgt tctaagctga cttccacggc agggagtggc
    18061 gataacagca aaaaaggtca agattcatgt cccccattga aaaatccagc aaattagaga
    18121 atgtctgtta tgacatccgt ggtccggtgc tgaaagaagc aaaacgcctg gaagaagaag
    18181 gtaacaaggt actgaaactg aacatcggca acccagcccc attcggtttt gacgcgccag
    18241 atgaaatcct cgttgacgtg atacgcaacc tgcctaccgc tcaagggtat tgcgattcca
    18301 aagggcttta ctccgcgcgt aaagccatca tgcagcacta ccaggctcgt ggcatgcgtg
    18361 atgttaccgt ggaagatatt tacatcggca atggtgtatc ggagcttatc gttcaggcaa
    18421 tgcaggcatt gctgaacagc ggggacgaaa tgttggttcc tgcaccagat tacccactct
    18481 ggaccgcggc ggtttcgctt tccagcggta aagcggtgca ttatctttgc gatgaatcct
    18541 ctgactggtt cccggacctc gatgatattc gcgctaaaat tacgcctcgt acgcgtggga
    18601 tcgttattat caacccaaat aacccaaccg gcgcggtata ttccaaagag cttttaatgg
    18661 agattgtgga gattgcacgt cagcataatc tcattatctt cgccgatgaa atttatgaca
    18721 aaattctcta cgacgatgct gagcatcact caattgcgcc actggcacct gacctgctga
    18781 ccattacctt taacggactg tcgaaaacgt accgcgttgc aggcttccgt caggggtgga
    18841 tggtgttgaa cgggccgaaa aaacacgcca aaggctacat cgaaggtctg gaaatgctgg
    18901 cttcaatgcg cctgtgtgct aacgttcctg cgcaacacgc cattcagacc gcgctgggtg
    18961 gttatcagag catcagtgaa tttattaccc ctggcggtcg tctttatgag cagcgtaacc
    19021 gcgcgtggga actgatcaac gatattccgg gcgtttcctg cgtgaaacct cgtggtgcgc
    19081 tgtatatgtt cccgaaaatc gacgccaaac gctttaacat tcacgacgat cagaaaatgg
    19141 tgctggattt cctgttgcag gaaaaagttc tgttggtgca agggacggca ttcaactggc
    19201 cgtggccgga tcacttccgc attgtcacgc taccgcgtgt cgatgatatc gagctgtctt
    19261 tgagcaagtt cgcgcgtttc ctttctggtt atcatcagct gtaatcttaa tttcactgcc
    19321 ggagattgca tccggcagcg ttatcccgcc acaatgacct gatgatgtca tcatacgtaa
    19381 ggtcactatg aaacagagcc atttttttgc ccatctctcc cgcctgaaac tcattaaccg
    19441 ctggccgcta atgcgcaacg tgcggacgga aaatgtgtcc gaacacagtt tgcaggtagc
    19501 gatggtcgcc catgcgctgg cagctatcaa aaatcgaaaa tttggcggta atgtcaacgc
    19561 cgaacgtatc gctttactgg cgatgtacca cgatgcctca gaagtgctca ccggcgatct
    19621 ccctactccg gtgaaatact tcaattcgca aatcgctcag gagtacaagg ctattgaaaa
    19681 aatcgctcag caaaaactgg tcaatatggt tccggaagag ctgcgggata tctttgcgcc
    19741 gttaattgac gagcatgcat atagcgatga agaaaaatcg ctggtgaaac aggcagatgc
    19801 actgtgtgca tatctgaaat gtctggaaga actcgcggcc ggaaataatg aattcttgct
    19861 ggcaaaaacg cgactggaag cgacgcttga agcgcgtcgc agccaggaga tggactactt
    19921 catggaagta tttgttccca gcttccatct ttcgctcgat gagattagcc aggattcacc
    19981 gctgtaagca gccggagtct gcgtcgcatc aggcaataag cgccggatgc gacatcaggc
    20041 tcttgtcaaa acggaaacag catcgggatc atcaccacac aaaccgccat cacgataatg
    20101 gtgaacggta cccccaactt cacaaagtca ctgaagctgt aatttcccgg acctaaaacc
    20161 agtgtgttaa caggtgaaga aaccggtgtc ataaaggcgg cggatgctgc catcgccacg
    20221 accatcgcga atggataagg cgacaccccc atcgttttgg cagcagccag cgcaatcgga
    20281 gccatcaaca ccgccgtcgc ggtattagag ataaatagcc caataaccgc cgacaagaca
    20341 aacaaacacc ccagcatcat atgtggcccg taaccaccgc caatgtccat cagccctttc
    20401 accgccagcg cgacacctcc cgttttctgt aatgccacag caaacggcat catcccaacg
    20461 atcaaaataa tgctcggcca gtgaatggat ttataggcgc tttcagcatc tatacagcgg
    20521 aatttcccca tcagcaggca ggcgatgata gcggcaacag gattaggaat ttcatctgtc
    20581 agcattaacg ccaccattag caccagacag aaaatggcat ggggtgcctg gctgtgcgcg
    20641 ggcgatgctt cactcacctc ttccggtaag ttcagcgcta cgaagtcgcg gccctgtttg
    20701 gccagcatac cgatcagttt ccagttaccc acaaccagga tgatatcgcc cagcagcaga
    20761 ggctcatccg ccagcgaacc ttccagcgcc acgccattgc gctttagccc caccacattc
    20821 agtccgtagc gggtacgaaa accaatttcg cgcaccgatt taccaatcag ttctgactca
    20881 ggaattaatg aaatctctgc catgcccaca tcaagggcct ggtcagaaaa atactcgccg
    20941 cgcagtacca tcggctccag caattgctca ctacaaaatt gccggagatc gacatcagcc
    21001 gcagacatat caataagcaa aacgtcacgc gcgcgaaatt cagaaacccc attaacgttc
    21061 acgataacgc gacgaaaacg ccgccagcgt tcaacaccga tgacgttagc gccataacgc
    21121 tcacgtaatt tgagatcatc cagccgttga ccaatcattg gcgatccggg gcgaatagcc
    21181 agacgtcgcg cacgcccggt cagtcgatat tcacggataa gatcgcgaaa ggttcgacgc
    21241 gtccagcctt cgcgctgcgg ggtctgggta tcccctttca gcatgaaacg catcactaac
    21301 atatacaaaa tacccagcac cagcacgacc aggccaatag gtgttacgct aaagaaactg
    21361 aagccgtgat agccttcacg cagcaattca ctgttgacta ccaggttcgg cggcgtcgcc
    21421 accagcgtca tcatgccgct tatcagcccg gcaaaactca acggcatcat cagacgtgac
    21481 ggcgaagttt gcatacgcat ggcaacgctt aacaccacgg gaataaagat agcgacaacg
    21541 ccggttgaac tcataaacgc gccaagcccc gcgacggtca gcatcaacaa aaccaacatt
    21601 ttgatttcac tgttgcccgc aactttgacc agccatgttc ccattacggt ggcaacaccg
    21661 gtacggacca aaccatcgcc aataataaac aaggcggcaa tcaggacaac gttaggatca
    21721 gaaaagccgg aaaatacttc ggggactgtc agcgttccgc ttaatgcaaa cgcgacaata
    21781 acaaacaaag cgaccgcatc catacgcact ctgcccgtcg caaacaagac aatggcaacg
    21841 gccagtaatg aaagaaccca aatcaattca ccgttcacaa cttatccttg ttaattgagg
    21901 gagatgtctt gattctgcca taaaaaagcc ccgacgagac ggggctaaat catgatcagg
    21961 tgtttcactg aataataaca tcgccatttg gctgtttggt cacagtaatt tgctccagac
    22021 tgtggaggac caaatcgacc tcattcaggc gcggggtatc tgccggagcg ttaaccgcaa
    22081 tgacatgaca acccgccgcc aggccagaaa gcacgccagc ggagcatctt ccaccaccac
    22141 acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat caggttctgg
    22201 ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa gcccagctat
    22261 tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc
    22321 tgctttattc aaatgactga gtaaggcgat tgcccccgga agcgggtaat accttcggtt
    22381 tccgtggcct cgatgtgctc cagacgcgta aactcggcgg caatatcagc ctcggatttg
    22441 cccgccataa aatggcgcag agaggtgatc gcctgtttac cgtgaatgaa agccagcacc
    22501 tcttccggcg ctaacccatg acgtctggcc cagttgctcc acgcccgttc taccgcaggc
    22561 agggaatcca ccagcgttcc atcaagatca aacagaaaac ctttgcaccg cacgcgggcc
    22621 tcctcaggca ttgataattt gattaatttc gttggcgctc aaatggtact gacgcgggca
    22681 ggcatgccac acattaagca tgcgctgata tttttcccac attggcgtct gggcgttaaa
    22741 accgtgagtt ccggcgtcaa aatgggtata gcgcccttcc acattaacca taaagcggac
    22801 ataaccgagg taacgtgctt cagtggcggc gtcaaagccg aggaaggtga cacgacgttc
    22861 atcgatggat tgctggtcct gcaaattaga ccaggaaaca tgcaacgcat gatacatctc
    22921 cataatgtcg atgatagtgc ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa
    22981 ttcacgcatc tgtaatccgt aacctcgctc aataattgtt tgcaggcgac ggtaacgttc
    23041 agcatttgcc ggatcgagca tagtcatcat cttgtactga ttagacaaaa taagacgttg
    23101 cgcgttggtc atttccattg ttgactcctg tatcactcta ctacggtgaa aaaaaagaag
    23161 gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca tcaaggaaag
    23221 ttttatccag ttgtttgaag gcgcgcttaa gcgtgtcagc taatgcctgg taatcaggct
    23281 tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga acttcataaa
    23341 accagttaag gattgcaggg ggtaatggcg ttacagaacg cttgcccagc caccacaatc
    23401 cctgcatggg taaacttaag gcgaacagtg cagtggcaac tgccggccca agctgaccgc
    23461 ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa cggatcgcat
    23521 aacgcgtcat cttgataacg cgattttcga caaagaccgg ggcaaggcgt ttttccagtg
    23581 gccacgtctt tgagtaatgc tgtccgcggc gaaacaagct aaaaaaatta acagaacgat
    23641 tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa atttgtgcaa
    23701 attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca ctatcaggta
    23761 tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa attcatatac
    23821 attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt tcttccatca
    23881 tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat catgcgctac
    23941 gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact tccatgtcga
    24001 gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc atcatcgatg
    24061 cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg cctgaagcac
    24121 gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca ggcgccgctc
    24181 acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca gaactgtctg
    24241 cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat accagctccg
    24301 tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt gcaccgctgc
    24361 acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca cagctgaaag
    24421 acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa gagtcttacc
    24481 tctacgccct gccttacaac ctgtacaaag agcacggcat ccgtcgttac ggcgcgcacg
    24541 gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa ccggtagaag
    24601 aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct atccgcaacg
    24661 gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc atgggtaccc
    24721 gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg ggcatgagcg
    24781 ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg accgaagtga
    24841 ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg aagcgcgcaa
    24901 tggacgttta ctgccaccgc ctggcgaaat acatcggtgc ctacactgcg ctgatggatg
    24961 gtcgtctgga cgctgttgta ttcactggtg gtatcggtga aaatgccgcg atggttcgtg
    25021 aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa cgcaacctgg
    25081 ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct gcggtggtta
    25141 tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact gcctgatttc
    25201 acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg gtaacgaaag
    25261 aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg tcggtctgac
    25321 cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc tgagcgtttt
    25381 caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta cgactatcgt
    25441 gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct acgttgaagg
    25501 tctgctttcc agcaatcaga aagatgtgct gatggaagag atcgtcgcaa actaccacgc
    25561 taacaccaaa gacgctgaag tcgttctggt tgaaggtctg gtcccgacac gtaagcacca
    25621 gtttgcccag tctctgaact acgaaatcgc taaaacgctg aatgcggaaa tcgtcttcgt
    25681 tatgtctcag ggcactgaca ccccggaaca gctgaaagag cgtatcgaac tgacccgcaa
    25741 cagcttcggc ggtgccaaaa acaccaacat caccggcgtt atcgttaaca aactgaacgc
    25801 accggttgat gaacagggtc gtactcgccc ggatctgtcc gagattttcg acgactcttc
    25861 caaagctaaa gtaaacaatg ttgatccggc gaagctgcaa gaatccagcc ctctgccggt
    25921 tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg atatggctcg
    25981 ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg ttaaatccgt
    26041 cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg gttctctgct
    26101 ggtgacttcc gcagaccgtc ctgacgtgct ggtggccgct tgcctggcag ccatgaacgg
    26161 cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc gcatttctaa
    26221 actgtgcgaa cgtgctttcg ctaccggcct gccggtattt atggtgaaca ccaacacctg
    26281 gcagacctct ctgagcctgc agagcttcaa cctggaagtt ccggttgacg atcacgaacg
    26341 tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga tcgaatctct
    26401 gactgccact tctgagcgca gccgtcgtct gtctccgcct gcgttccgtt atcagctgac
    26461 tgaacttgcg cgcaaagcgg gcaaacgtat cgtactgccg gaaggtgacg aaccgcgtac
    26521 cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac tgctgggtaa
    26581 tccggcagag atcaaccgtg ttgcagcgtc tcagggtgta gaactgggtg cagggattga
    26641 aatcgttgat ccagaagtgg ttcgcgaaag ctatgttggt cgtctggtcg aactgcgtaa
    26701 gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg tggtgctcgg
    26761 tacgctgatg ctggaacagg atgaagttga tggtctggtt tccggtgctg ttcacactac
    26821 cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca gctccctggt
    26881 atcttccgtg ttcttcatgc tgctgccgga acaggtttac gtttacggtg actgtgcgat
    26941 caacccggat ccgaccgctg aacagctggc agaaatcgcg attcagtccg ctgattccgc
    27001 tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg gtacttctgg
    27061 tgcaggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg aaaaacgtcc
    27121 tgacctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg acgttgcgaa
    27181 atccaaagcg ccgaactctc cggttgcagg tcgcgctacc gtgtttatct tcccggatct
    27241 gaacaccggt aacaccacct acaaagcggt acagcgttct gccgacctga tctccatcgg
    27301 gccgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg cactggttga
    27361 cgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc agtaacctcg
    27421 tcatcatccg cagctttgcg ctgcggatat ctgaaccgga aataatcact atttccggtt
    27481 ttttattctc ttaatctgca ttaatccttt ctgattatct tgcttaactg cgctgcatca
    27541 atgaattgcg ccatttcact ttacatactt accactttgt tttgtgcaag ggaatatttg
    27601 cgctatgtcc gcaatcactg aatccaaacc aacaagaaga tgggcaatgc ccgatacgtt
    27661 ggtgattatc ttttttgttg ctattttaac cagccttgcc acctgggtag ttccggtggg
    27721 gatgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa cacgcaaagt
    27781 cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg agtatcaccg
    27841 cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc cgtttgaagg
    27901 attaacctca ggatcgaaat acgggacagc tgttggcatc atcatgttta tgctggtgat
    27961 tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta tcctggcgct
    28021 tattcgccat acccgcggga atgaaattct ctttattcct gcgctgttta ttctgttttc
    28081 acttggcggc gcggtatttg gtatgggaga agaggccgtc gcctttgcca ttatcatcgc
    28141 accgctaatg gtccggctgg gctatgacag tattaccacc gtcctggtga cctatattgc
    28201 cacgcaaatc ggttttgcca gttcgtggat gaacccgttt tgtgtggtcg ttgctcaggg
    28261 gattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt gggttatcgc
    28321 cactatgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa agaatcctct
    28381 tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggcgg atgttgaaca
    28441 acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg taatggtctg
    28501 ggtgatttgg ggcgtgatcg ttaatgcctg gtttattcca gaaattgcca gccagttctt
    28561 caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg gcatgacggt
    28621 taataccatg gcttcatcct ttaccgaagg gacgcgaatg atgatcgccc ctgccctgct
    28681 ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg gtgatgccag
    28741 cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg acaacgcggt
    28801 cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga cgtccggttc
    28861 tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg tcggtgttaa
    28921 ccgtcaggtt accgtgctgg cattccagtt tggtgatggc ttcagtcaca tcatttaccc
    28981 aacctcagct tcgttaatgg caactctcgg tgtgtgcagg gtggacttcc gtaactggct
    29041 gaaggtgggc gcgaccctac ttggactgct gtttattatg tccagcgtcg tagtgatcgg
    29101 cgctcagttg atgggctacc actaaaaatg ttaagagccg caattgcggc tcttttttca
    29161 ttctgccgtt tcagtctcta cggcttcatt tttggcattg cgcttcatcc acaacgccag
    29221 cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt ccggcgtcag
    29281 ccagcaaact tcactgactt catcttcctg tagggcgaag ggaccgtgag agacgcagct
    29341 gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc cgtgctcggc
    29401 aaaggggaca ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg attccagcag
    29461 ttgctcatcg gcctgaacca caccgcctgc ggtcgcatct aacatgccgg gtaaaaagtc
    29521 ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca cgacgatgta
    29581 agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt gtgcaatgac
    29641 ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac gctgttccat
    29701 tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt gggtaaatta
    29761 cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta cactaagtac
    29821 gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac tcaccgagcc
    29881 ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg tatgaccgta
    29941 caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa gatgaccatg
    30001 cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg ttatcgggaa
    30061 atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga taaccttatg
    30121 tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg gtaaagcatt
    30181 acgcgggccg tgattcaaca cgtcgccaag gatcaccagc cactgggcac cgctttgggc
    30241 aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg atgcaaacat
    30301 cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga ttctgtcgtt
    30361 gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac gctcgctgcc
    30421 actggaatgt cgcataatcc accaggcgtt ctggcacctc atcgccatgt agtaccaggc
    30481 ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat aaattcggct
    30541 gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg gcttttccct
    30601 cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcgga cgctcttcgc
    30661 ggatgggcat cagatcgctg cgcagccagg cctgaatccg tcgtgcacgc gcacgatttt
    30721 ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct tccagatact
    30781 ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac ggcacacggc
    30841 gtgtctgacc gtaaccttgc cacgtcggct tcaaatgttc accgctgtcg aggtcgatgg
    30901 tcttgatatg aaacgacagg cctttttcct gcaacgccac ccaggcggat aacacataag
    30961 gggagaaaaa gtgggcatct gaccaaagcg tgatagcggg tttactcatt atgtcctcgg
    31021 caataaaggg ggctggctat caaactatag agcctttttg tcactgtcac gtgacgaata
    31081 ctcacgtaag tcgccgcgct ttctatactt gtggtgttca gatgattaaa caaccggagc
    31141 tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta cgctgtttct
    31201 cgaagaagca gagcttgatt atcgcttgat taaggtagac ctggggaaag gcggtcagtt
    31261 tcgtccggaa tttttgcgca tttcgcccaa caacaaaatt ccggcaattg ttgatcattc
    31321 tcctgccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt tgttgtatct
    31381 gactgagaaa acaggactct ttttgagtca tgaaacgcgt gagcgcgccg ccacattaca
    31441 gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc atcattttaa
    31501 tcacgcagcc ccccaaacca ttccttacgc tattgaacgt tatcaggttg aaactcagcg
    31561 tctttaccat gtactaaaca agcggctgga aaactcgccc tggctgggag gcgagaacta
    31621 cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc agcgaattga
    31681 cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc gccctgccac
    31741 cgggcaggca ctgctaaaag cacaactcgg tgatgagcgt tcggatagtt aacagaaaca
    31801 ggttctcgtg tattatttca tcctaagtaa aacaacggag aacctgcaat ggcacaacct
    31861 gccgctatta ttcgtataaa gaaccttcgt ttacgtacgt ttatcggaat taaggaagaa
    31921 gaaattaaca accgtcagga tattgttatc aatgtgacga tccactaccc cgccgataaa
    31981 gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac gaaaaacatt
    32041 attcagcatg tagagaataa ccgtttctct ttgttggaaa aattaactca ggatgtgctc
    32101 gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga taaactgcac
    32161 gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta accgccatat
    32221 cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt cgccatttga
    32281 ttccgcgttt gctggagctg ggccatcaaa tcacggtagt gacgcgtaac ccacagaaag
    32341 ccagttccgt tctcggccct cgggtgacgc tatggcaagg gctagccgat caaagcaacc
    32401 tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat aaacgctgga
    32461 ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa aaactggtcg
    32521 atttgattaa tgccagcgac acgccgccgt cggtattcat ttccggctcg gcaacgggct
    32581 attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat aacgaattta
    32641 ctcataaact ctgcgcccgc tgggaagaga ttgcctgccg ggcgcaaagt gacaaaacgc
    32701 gagtgtgcct gctgcgaacc ggcgtggtac tggcaccgga cggcggtatt ctcggtaaaa
    32761 tgctgccacc gtttcgtctt ggcctgggcg ggccgattgg ttccggtcgg cagtatctgg
    32821 cctggattca tattgatgat atggtcaacg gcattctctg gctgctggat aacgacctgc
    32881 gcgggccatt taatatggtt tcgccctacc cggtacgcaa tgaacaattt gcccatgcgc
    32941 tcggtcaagc gctgcatcgc ccggccattt tgcgcgtccc cgcaaccgcc attcggctgt
    33001 taatgggcga atcttcaata ctggtattag gtggacaacg cgcgctgcct aaacggctgg
    33061 aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg gatgtcgttc
    33121 gctgatgtgg tttacagcaa acatccgcca gttaactccc ggtgttacag gattagtggc
    33181 tttgcgcgat aagatcgtct ggtgaaagtc gggtcaccat cataactaac tctctgtcta
    33241 aacctctatc cagcatctcc tgagcaatac gcagggcttc ttcgtgtttg ccctgcattg
    33301 cgccttcttc acgtaatctg tcagcaatgg tcatcagttt ctccttttct tgtggtgcgc
    33361 gttctgttat ctcaccaata aatgcacgga aacgctgggc atcccctgtt tgtaatacgt
    33421 aattaaacag ggcttttagc tgtctgtcat tagtgttccc tgtaactagc agcgaaacaa
    33481 tttggtcgac taatcccaac agatcgcgct gacgaatatg tttctgaatt aactccaaca
    33541 gcgccatttt gcggtgttgc ataatctcgt catccggcac cacggtaata tccaccaacg
    33601 gaaacgctga tgaatatatt ttgcgggcta tagcaggctc ggcaaattca tcaagccagc
    33661 agagtgaata aggataagga cttctgcaac catgataaaa cagcatcggg atcaccaatg
    33721 gaagctcttt atagcccgca tcaagatggt tttgcattgc cgcaatggaa taacgcatca
    33781 tgcgaaaagc cattaattct tccagcttac tttggtgctc tatcactaca taaatataac
    33841 ccactccctc ctgcgttttc acagaccaca agaggtcgga ataatattgc cgcaggtctt
    33901 catcaataaa actgtttgat tctagtttaa gcgtcgttaa atcacacagt ttgcgcagcg
    33961 gcgcgggaag atgaatatca ataaaatccc gcgcggtgt
//
LOCUS       DAZSBF010000021        33016 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZSBF010000021 DAZSBF010000000
VERSION     DAZSBF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.021"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..852)
                     /gene="melB"
                     /locus_tag="ACHXWD_000746"
     CDS             complement(<1..852)
                     /gene="melB"
                     /locus_tag="ACHXWD_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR7619746.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLV"
     gene            complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHXWD_000747"
     CDS             complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHXWD_000747"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR7619747.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2593..3501
                     /gene="melR"
                     /locus_tag="ACHXWD_000748"
     CDS             2593..3501
                     /gene="melR"
                     /locus_tag="ACHXWD_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR7619748.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3700..5967
                     /gene="adiA"
                     /locus_tag="ACHXWD_000749"
     CDS             3700..5967
                     /gene="adiA"
                     /locus_tag="ACHXWD_000749"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR7619749.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            6292..7053
                     /gene="adiY"
                     /locus_tag="ACHXWD_000750"
     CDS             6292..7053
                     /gene="adiY"
                     /locus_tag="ACHXWD_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR7619750.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            7190..8527
                     /gene="adiC"
                     /locus_tag="ACHXWD_000751"
     CDS             7190..8527
                     /gene="adiC"
                     /locus_tag="ACHXWD_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR7619751.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            8631..10274
                     /gene="eptA"
                     /locus_tag="ACHXWD_000752"
     CDS             8631..10274
                     /gene="eptA"
                     /locus_tag="ACHXWD_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR7619752.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            10271..10939
                     /gene="basR"
                     /locus_tag="ACHXWD_000753"
     CDS             10271..10939
                     /gene="basR"
                     /locus_tag="ACHXWD_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR7619753.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            10940..12040
                     /gene="pmrB"
                     /locus_tag="ACHXWD_000754"
     CDS             10940..12040
                     /gene="pmrB"
                     /locus_tag="ACHXWD_000754"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR7619754.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(12016..12105)
                     /gene="pmrR"
                     /locus_tag="ACHXWD_000755"
     CDS             complement(12016..12105)
                     /gene="pmrR"
                     /locus_tag="ACHXWD_000755"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR7619755.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(12217..13719)
                     /gene="proP"
                     /locus_tag="ACHXWD_000756"
     CDS             complement(12217..13719)
                     /gene="proP"
                     /locus_tag="ACHXWD_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR7619756.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(13983..14849)
                     /locus_tag="ACHXWD_000757"
     CDS             complement(13983..14849)
                     /locus_tag="ACHXWD_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR7619757.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(14846..17074)
                     /gene="crfC"
                     /locus_tag="ACHXWD_000758"
     CDS             complement(14846..17074)
                     /gene="crfC"
                     /locus_tag="ACHXWD_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR7619758.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            17487..17822
                     /locus_tag="ACHXWD_000759"
     CDS             17487..17822
                     /locus_tag="ACHXWD_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR7619759.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            17980..18423
                     /gene="yjdN"
                     /locus_tag="ACHXWD_000760"
                     /pseudo
     CDS             17980..18423
                     /gene="yjdN"
                     /locus_tag="ACHXWD_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            18556..19344
                     /gene="phnC"
                     /locus_tag="ACHXWD_000761"
     CDS             18556..19344
                     /gene="phnC"
                     /locus_tag="ACHXWD_000761"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR7619760.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            19369..20385
                     /gene="phnD"
                     /locus_tag="ACHXWD_000762"
     CDS             19369..20385
                     /gene="phnD"
                     /locus_tag="ACHXWD_000762"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7619761.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            20511..21290
                     /gene="phnE"
                     /locus_tag="ACHXWD_000763"
     CDS             20511..21290
                     /gene="phnE"
                     /locus_tag="ACHXWD_000763"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR7619762.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            21311..22036
                     /gene="phnF"
                     /locus_tag="ACHXWD_000764"
     CDS             21311..22036
                     /gene="phnF"
                     /locus_tag="ACHXWD_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR7619763.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            22037..22489
                     /gene="phnG"
                     /locus_tag="ACHXWD_000765"
     CDS             22037..22489
                     /gene="phnG"
                     /locus_tag="ACHXWD_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR7619764.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            22486..23070
                     /gene="phnH"
                     /locus_tag="ACHXWD_000766"
     CDS             22486..23070
                     /gene="phnH"
                     /locus_tag="ACHXWD_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR7619765.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            23070..24134
                     /gene="phnI"
                     /locus_tag="ACHXWD_000767"
     CDS             23070..24134
                     /gene="phnI"
                     /locus_tag="ACHXWD_000767"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR7619766.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            24127..24972
                     /gene="phnJ"
                     /locus_tag="ACHXWD_000768"
     CDS             24127..24972
                     /gene="phnJ"
                     /locus_tag="ACHXWD_000768"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR7619767.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            24969..25727
                     /gene="phnK"
                     /locus_tag="ACHXWD_000769"
     CDS             24969..25727
                     /gene="phnK"
                     /locus_tag="ACHXWD_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR7619768.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            25838..26518
                     /gene="phnL"
                     /locus_tag="ACHXWD_000770"
     CDS             25838..26518
                     /gene="phnL"
                     /locus_tag="ACHXWD_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR7619769.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            26515..27651
                     /gene="phnM"
                     /locus_tag="ACHXWD_000771"
     CDS             26515..27651
                     /gene="phnM"
                     /locus_tag="ACHXWD_000771"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR7619770.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            27651..28208
                     /gene="phnN"
                     /locus_tag="ACHXWD_000772"
     CDS             27651..28208
                     /gene="phnN"
                     /locus_tag="ACHXWD_000772"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR7619771.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            28195..28629
                     /gene="phnO"
                     /locus_tag="ACHXWD_000773"
     CDS             28195..28629
                     /gene="phnO"
                     /locus_tag="ACHXWD_000773"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR7619772.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            28631..29389
                     /gene="phnP"
                     /locus_tag="ACHXWD_000774"
     CDS             28631..29389
                     /gene="phnP"
                     /locus_tag="ACHXWD_000774"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR7619773.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(29536..29865)
                     /gene="yjdP"
                     /locus_tag="ACHXWD_000775"
     CDS             complement(29536..29865)
                     /gene="yjdP"
                     /locus_tag="ACHXWD_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR7619774.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(29934..30251)
                     /locus_tag="ACHXWD_000776"
     CDS             complement(29934..30251)
                     /locus_tag="ACHXWD_000776"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR7619775.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            30504..32489
                     /gene="yjcS"
                     /locus_tag="ACHXWD_000777"
     CDS             30504..32489
                     /gene="yjcS"
                     /locus_tag="ACHXWD_000777"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR7619776.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            32698..32973
                     /locus_tag="ACHXWD_000778"
     CDS             32698..32973
                     /locus_tag="ACHXWD_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR7619777.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         8026 a         9109 c         8460 g         7421 t
ORIGIN      
        1 tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga acaaatccgc
       61 atcaccgata acatatgaga aataatagat agcaaatccg gtaataatgt tgctggctac
      121 attataagca agagccatac ccaagaggca tgaaagctga tcgtttttat aaattagcgc
      181 aacgatggct ttaagtgtca gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa
      241 gacttcatgc acattgcgca gagtgatgat ggttgaaaca ataaaaaagg cgatcagtac
      301 cagtgtgaac atctgaaagc caaatccccg atcgccaccg ccgacataat taacaaatgg
      361 tagcgtcaca cctgccgtaa caaagcccgc cagactggca aaaaaacgcg gataaggaac
      421 cagttgttca cgctcacgtt tatcgagcgt gatggttgga accagcgacc agaagggaat
      481 atccataatg gtgtaagtca tgccccagag gatgtaggtc acgcaaacaa agacaatctg
      541 agtagtacct tcaaacagat gcgcactaaa gaggagaaat aagattacag agtttgccaa
      601 cgtaccgatc aggatccagg gtttgaactt accccatcgc gatcgcgtag catttacaat
      661 ccatcccata atcggatcgt taatagcatc ccagatcctc gccaccagaa acaaagtacc
      721 gaccaaaccc acagacagcc caacgacatc ggtgtagtaa tacatgaggt acatatacac
      781 aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca taactgagtt ttgtagtcat
      841 tgaaatgctc atagggtatc gggtcgctgt taccaacgcc cgccttctgt tattggtatc
      901 gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag cgtttagtcg cgttttaacg
      961 gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata
     1021 tatttcgtca atgcccagca cggcggcagt atgcgggtcc atcatcgcgg cgtggtaaac
     1081 acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc gtctgtacat tgatgttggt
     1141 ttgcatcagg gcggccagat gcgaaggtag cgtaccgact ttagtcggct gaatgccatt
     1201 agcatcaacc agacaggcta cttccacgca acatccttgt ggcaggttat caatcaaacc
     1261 atcgttacgg acgttgccgt aaatcacact cggctcgcca gtccagatag cgttcatgat
     1321 tgtgctggca tattctcgtg acggtttaat atcaatccgg gaggcgtttt tatactcctc
     1381 cagctcttta tgccagttcg ccagctgctc gacgcagcgt ttcgggtact catccagcgg
     1441 tactttataa cgctcaatca aatcctcacg acctggctta ataaaccacg gcgtgtattc
     1501 agcaaaatgt tctgacgatt ccgtaacgaa gtagcccagc tttttgaaca tttcgtagcg
     1561 cacaatattc tggcagcgag tattaccatg aatatttggc ttcggtgcct gccctgcttc
     1621 ataagccgcc agcagttccg ggtagagatt cacgtaactg ccgtcggcgg ttttacgctc
     1681 cagttccagg taaaacgcca tatggttgat accagcgcaa cggtaacgca gcgtggacgg
     1741 atcgatattg aggtcacgcg ccagctcttc cgccgttccc tgcaccgaat ggcacagccc
     1801 tacctgtttg atatgcagat agcgggcata catcgcccag gtattcatcg ccattgggtt
     1861 aacgtagttg agcatggtgg catcggggca gacttccgtc atgtcctcgc aaatttgcca
     1921 cagatgcgga atggtacgta gcgcgcgcat aataccgccc ggccccaacg tatcggcaat
     1981 ggtttgttcc agaccatgcc gcttacagac ctcgaaatca gtcaccgtgc aaggttcata
     2041 accgccaatc tgaaatgcca ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg
     2101 ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc atcagcttac gcaccacaat
     2161 atgcgactct tccaggcggg tgggatcaat gtccatcagg gcaatatgcg ccgttttcag
     2221 cgcctcgcga tggaacacat caccaagaat atttttaacg aaaatcgtcg aaccagcgcc
     2281 gataaatgta attttgggtg cagtcatcat gcagatctcc tggcttgctt gaataacttc
     2341 atcatggcag gcgaatctgc agaaaacctc cggcttccag gttgagcatt cccataaact
     2401 cagatcctgc gtgaagcagc agtaaatctg agtttatggt aataagttac ctgaaaagca
     2461 gagttttcgg cctcatgatg ctcggacgct cccctcgcct ctctgggaag agggtgaaaa
     2521 ccgtgctccc actcgcagtc atcctccctc actcctgcca taattctgat attccaggaa
     2581 agagagccat ccatgaatac agatacgttt atgtgcagca gcgacgaaaa acagacccgc
     2641 agcccgctgt cgctgtattc ggaataccag cggatggaga ttgagtttcg cgcaccgcat
     2701 atcatgccca ccagccactg gcatggtcaa gtcgaagtga atgtgccttt cgatggcgat
     2761 gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc agggtaatat cacgctgttc
     2821 tgggcctgta caccgcacca actaacagat accggaacct gtcagagcat ggcgattttt
     2881 aatctgccga tgcatctgtt tctctcctgg ccgctggata aagacctgat taaccacgtc
     2941 actcacggca tggtgatcaa atcactggcc acacagcaac ttagcccgtt tgaagtgcgc
     3001 cgctggcagc aggaattaaa cagtccgaac gagcaaattc gccagctcgc cattgatgaa
     3061 attggcctga tgctcaagcg atttagcctc tctggctggg aaccgattct ggtcaataaa
     3121 acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc aattttatgt tagccagatg
     3181 ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca tcaacgatgt ggctgagcac
     3241 gtcaaactta acgccaacta tgcaatgggg atattccagc gggtcatgca attgacgatg
     3301 aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg cgttactgag cgataccgat
     3361 aaaagtattc tcgatattgc cctgacggca ggctttcgtt caagtagtcg tttttacagc
     3421 acgttcggca aatatgtcgg catgtcgccg caacaatacc gcaaacttag ccaacagcgc
     3481 cgccagacgt ttcccggcta aagacgatat cagtatcagc caaaaaaata gtttagccgt
     3541 cgataataag cagtgaaaat aatttaaaat aatcacataa cgcattgttt ataaaaataa
     3601 atatttcaaa aatgtttgtt tttcacgcgc tttacagccc gaaaaggccg gaagatactt
     3661 gcccgcaacg aagattcctt cataaccggg taagcaatga tgaaagtatt aattgttgaa
     3721 agcgagtttc tccatcaaga cacctgggtc ggtaacgccg ttgagcgtct ggcagatgct
     3781 ttaagccagc aaaatgttac cgtgattaaa tccacctcct ttgatgatgg ttttgccatt
     3841 ctctcttcaa acgaagccat tgactgcctg atgttcagct atcaaatgga acatccggac
     3901 gaacatcaaa acgtcagaca attgatcggt aagcttcatg agcgccaaca aaacgtgccg
     3961 gtcttcctgt tgggcgatcg ggaaaaagcc ctcgccgcaa tggatcgcga cctgctggag
     4021 cttgtcgatg aattcgcctg gattctggaa gataccgccg actttatcgc cggacgcgct
     4081 gttgccgcga tgacccgcta ccgccagcag ctgttgccgc cactgttcag cgcgctgatg
     4141 aaatatagcg acatccatga atattcctgg gcagcgccag gccaccaggg cggcgttggt
     4201 tttaccaaaa cacccgccgg acgtttctac catgactact atggtgaaaa tctgttccgc
     4261 accgatatgg gcatcgaacg aacttccctc ggttctttgc ttgaccatac tggcgcattt
     4321 ggcgaaagcg aaaaatatgc cgcacgcgta tttggtgccg atcgctcctg gtcggtagtc
     4381 gtcggtactt ccggctctaa ccgcaccatc atgcaggctt gcatgaccga taacgatgtc
     4441 gtggtcgttg accgtaactg ccataaatcc atcgaacaag gcttgatgct gacaggcgcg
     4501 aaaccggtct acatggtgcc aagccgcaac cgctacggca ttatcgggcc aatctatccg
     4561 caggaaatgc aacctgaaac cttgcagaag aaaatcagtg aaagcccgct gactaaagac
     4621 aaagccgggc aaaaaccgtc ttactgcgtg gtgaccaact gcacctatga cggcgtgtgt
     4681 tataacgcta aagaagcgca ggatctgctg gaaaaaacct ccgatcgtct gcactttgac
     4741 gaagcctggt acggctatgc acgtttcaac ccgatctatg ccgatcacta tgccatgcgc
     4801 ggcgaacctg gcgatcacaa cggtcctacc gttttcgcca cccactccac ccacaaactg
     4861 ctgaatgcgc tttcacaggc ttcttatatt catgtacgtg aaggtcgtgg ggcgattaac
     4921 ttctcccgct tcaaccaggc ctacatgatg catgccacca cctccccgct gtatgccatc
     4981 tgcgcatcca acgacgtggc ggtgtcgatg atggacggca acagcggcct gtcactgacg
     5041 caggaagtga tcgacgaagc ggttgatttc cgtcaggcga tggcgcggct gtataaagag
     5101 ttcaccgctg acggtagctg gttcttcaaa ccgtggaaca aagaagtcgt caccgaccca
     5161 caaaccggca aaacctatga ctttgctgac gcaccaacca aactgctgac caccgttcag
     5221 gactgctggg taatgcatcc gggcgaaagc tggcacggct tcaaagatat tccggataac
     5281 tggagtatgc tcgacccgat taaagtcagc atccttgctc cgggaatggg tgaagatggt
     5341 gaactggaag aaaccggtgt tccggcggcg ctggtcactg cctggcttgg tcgccacggc
     5401 attgtgccta cccgcaccac tgacttccaa attatgttcc tgttctctat gggcgtaacc
     5461 cgtgggaaat ggggaactct ggttaacacc ctttgctcct tcaaacgcca ctatgacgcc
     5521 aacacaccgc tggcgcaggt gatgccggaa cttgttgaac aatatcctga cacttacgcg
     5581 aacatgggga ttcacgatct gggtgacacc atgtttgcct ggctgaaaga aaacaaccct
     5641 ggcgcacggt tgaacgaagc ctattccggc ctgtcggtgg cggaaatcac cccgcgtgaa
     5701 gcgtacaacg cgattgtcga caacaatgtc gaactggtat ccattgaaaa tctgccagga
     5761 cgcatcgcgg caaactcagt tatcccatat ccgccaggaa tcccgatgct gctgtctggt
     5821 gaaaacttcg gcgataaaaa cagtccgcaa gtaagttatt tacgctcgct gcaatcctgg
     5881 gaccaccatt tccctggatt tgaacacgaa actgaaggga ctgaaattat tgacggtatt
     5941 taccacgtta tgtgtgtgaa agcgtaacca ctattccgct gaaggcgtaa ttgtttaaat
     6001 aacattacgc cgcctggcct taggcctttt gagtatggca acgttttcat aaaaattgct
     6061 gcaaacaaaa atgtcatact ttttgcgcgg ccccaccccg cgcttttttt tgcctgttat
     6121 ttatcctgta aaaaaatatg tacatgagaa attactataa aaatttgtaa tattagtaaa
     6181 actcgttatt tttatgcatg tttatattca tcatacaatt atataaccat ttcccggtat
     6241 cgctttgctt tagcgagaac tggtctttta ttcgcactca ggagtacatg tatgaggatt
     6301 tgcagcgacc aaccttgtat tgttttattg actgaaaaag atgtctggat aagggtgaat
     6361 gggaaagaac ctattagcct taaagctaac catatggcgt tattaaattg tgaaaataat
     6421 attatcgacg tctcctctct taacaacact ttggttgctc atattagtca cgacatcatc
     6481 aaagattacc tccggtttct gaataaagat ctctcgcaaa taccagtatg gcaacgtagc
     6541 gctacgccca tactcaccct gccatgcctg acgccagacg tctttcgcgt tgccgcgcaa
     6601 cacagcatga tgcccgcaga aactgagtca gaaaaggaac gaacacgcgc attattattc
     6661 acggtgctat cccgttttct cgacagtaaa aaattccttt cattaatgat gtatatgtta
     6721 cgtaattgtg taagtgacag cgtttatcaa attattgaaa gcgatattca caaagactgg
     6781 aatcttagta tggtagccag ttgtttatgt cttagcccaa gtctgttaaa gaaaaagctg
     6841 aaaagcgaaa acaccagtta tagccaaata atcaccacct gccgcatgcg ttatgccgta
     6901 aatgaattaa tgatggacgg taaaaatatc tctcaggtat cacagtcctg cggctacaac
     6961 agtacgtcgt actttatttc tgtctttaaa gacttctacg gtatgacgcc gctgcattat
     7021 gttagtcagc acagagaacg aactgtcgcc tgatttttaa ccttaacgaa gagctatatt
     7081 aataacggca tcagcgataa cccggtcgat aataattcaa ctatcgaatg caggcgtatg
     7141 atatgacgta atttattgtc acgaagctcg ccttcgcagg agtttaatta tgtcttcgga
     7201 tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg atggtgtcgg ggaatattat
     7261 ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc actggcggga ttgccattta
     7321 tggatggttg gtgacgatta tcggtgcgct ggggctctcg atggtatacg ccaaaatgtc
     7381 gttcctcgat ccaagtcctg gtggttctta cgcttacgcc cgccgctgct ttggcccgtt
     7441 tctcggttat caaaccaacg tcctctactg gctggcctgc tggatcggca atatcgctat
     7501 ggtggtcatt ggcgtaggat atttaagtta cttcttcccg attctgaaag atccattggt
     7561 attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc ctgctgaaca ttgtcggtcc
     7621 gaaaatgatc acccgtgtgc aggccgttgc caccgtgctg gcgctgatcc ccatcgtcgg
     7681 gattgccgta tttggctggt tctggttccg tggtgaaacc tatatggcgg catggaacgt
     7741 cagcggcctg ggcaccttcg gtgcgattca aagtaccctt aacgttacgc tgtggtcgtt
     7801 catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg aaaaacccga aacgcaatgt
     7861 ccctatcgcc accattggtg gggtattgat tgccgccgtt tgctatgtgc tctccaccac
     7921 cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt tctgcctcgc cattcggtga
     7981 tgccgcacgg atggcgttgg gtgacaccgc tggggccatt gtttccttct gcgcagctgc
     8041 gggttgctta ggttcactgg gcggctggac gttgctggcg ggtcaaacgg cgaaagccgc
     8101 tgccgatgac ggactgttcc caccgatttt tgcccgtgta aataaagcgg gtacgccagt
     8161 ggcggggctg attatcgtcg gtattttgat gaccatcttc cagctcagta gcatttcgcc
     8221 aaacgcaacc aaagagttcg gtctggtatc ttccgtgtcg gtcatcttta cactggtgcc
     8281 atatctttac acctgtgcgg cgttactgct gctcggccac ggtcactttg gtaaagcacg
     8341 cccggcatat ctggctgtta ctaccattgc cttcctctac tgcatctggg ccgtggtggg
     8401 gtccggggcg aaagaggtta tgtggtcatt tgtcaccctg atggtcatca ccgccatgta
     8461 tgcgctgaat tacaaccggc tacataaaaa cccgtatccc ttagatgcac caataagcaa
     8521 agattaattc cccttaatcc agcaaacata aaagccaacc ttaagaactt aaggttggct
     8581 taattttgct ttgcgagcat atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc
     8641 gcctactaaa aagcccctct ttgaatttac tcgcctggct attgttggcc gctttttata
     8701 tctctatctg cctgaatatt gcctttttta aacaggtgtt gcaggcgctg ccgctggatt
     8761 cgctgcataa cgtactggtt ttcttgtcga tgccggtcgt cgctttcagc gtgattaata
     8821 ttgtcctgac actaagctct ttcttatggc ttaatcgacc actggcctgc ctgtttattc
     8881 tggttggcgc ggctgcacaa tatttcataa tgacttacgg catcgtcatc gaccgctcga
     8941 tgattgccaa tattattgat accactccag cagaaagtta tgcgctgatg acaccgcaaa
     9001 tgttattaac gctgggattc agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa
     9061 tcaaacctgc cacctcgcgt ctgcgcagtg ttcttttccg tggagccaat attctggttt
     9121 ctgtactgct gattttgctg gtcgccgcac tgttttataa agactacgcc tcgttgttcc
     9181 gcaataacaa agagctggtg aaatccttaa gcccctctaa cagcattgtt gccagctggt
     9241 catggtactc ccatcagcga ctggcaaatc taccgctggt gcgaattggt gaagacgccc
     9301 accgcaaccc gttaatgcag aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg
     9361 aaacctcgcg ggcggagaac ttctccctca acggctaccc gcgtgaaact aacccgcggc
     9421 tggcgaaaga taacgtggtc tatttcccta ataccgcatc ttgcggcacg gcaacggcag
     9481 tttcagtacc gtgcatgttc tcggatatgc cgcgtgagca ttacaaagaa gagctggcac
     9541 agcaccagga aggcgtgctg gatatcattc agcaagcggg catcaacgtg ctgtggaatg
     9601 acaacgatgg cggctgtaaa ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc
     9661 tgaatctgcc tggtcagtgc atcaacggcg aatgctatga cgaagtgctg ttccacgggc
     9721 tggaagagta catcaataac ctgcagggtg atggcgtgat tgtcttacac accatcggca
     9781 gccacggtcc gacctattac aaccgctatc cgccgcagtt caggaaattt accccaacct
     9841 gcgacaccaa tgagatccag acctgtacca aagagcaact ggtgaacact tacgacaaca
     9901 cgctggttta cgtcgactat attgttgata aagcgattaa tctgctgaaa gaacatcagg
     9961 ataaatttac caccagcctg gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg
    10021 gcatctatct gcacggtctg ccttatgcca tcgccccgga tagccaaaaa caggtgccga
    10081 tgctgctgtg gctgtcggag gattatcaaa aacggtatca ggttgaccag aactgcctgc
    10141 aaaaacaggc gcaaacgcaa cactattcac aagacaattt attctccacg ctattgggat
    10201 taactggcgt tgagacgaag tattaccagg ctgcggatga tgttctgcaa acttgcagga
    10261 gagtgagtga atgaaaattc tgattgttga agacgatacg ctgttattgc agggactgat
    10321 tctggcggcg caaaccgaag gctacacgtg cgatggcgta acaaccgcgc ggatggcgga
    10381 acaaagcctt gaggccggtc attacagcct ggtggtactg gatttagggt tacccgacga
    10441 agatggactg cattttctcg cccgtatccg gcagaaaaaa tacaccctgc cggtactgat
    10501 cctcaccgct cgcgatacgc tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga
    10561 ctatctggtg aagccttttg cgctggaaga gttacacgcc cgtatccgcg ccctgctacg
    10621 acgccataat aatcagggcg aaagtgagct gattgttggc aatctgacgc tgaatatggg
    10681 tcgccgtcag gtatggatgg gcggtgaaga gttgattctg acgcccaaag aatatgctct
    10741 gctgtcacgg ttaatgctca aagcaggcag tccggtacat cgggaaattc tctacaacga
    10801 catctataac tgggacaatg aaccctcgac caacaccctg gaagtgcata tccacaatct
    10861 gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc ggctttggct atatgctggt
    10921 cgcgaatgag gaaaactaat tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg
    10981 ccaacggctg atattgacca tcggggccat tttgttggtg tttgagctga tcagcatctt
    11041 ctggctatgg catgaaagta ccgagcagat tcagctgttt gagcaggcgc tacgcgacaa
    11101 tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt
    11161 ccccggcgtc tttatggtca gcctgacgct atttatctgc tatcaggcgg tacgccgcat
    11221 cacccgcccg ctggcggagc tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac
    11281 gcccattgcc attcacagcg ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga
    11341 tctggtcagt cgcctgacga gcacgctgga taacgaaagg ttatttaccg ctgacgtcgc
    11401 gcacgaactg cgaacgccac tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac
    11461 gcatcacatt gatgtagcac cgttagtggc acggcttgat cagatgatgg agagcgtctc
    11521 ccagctgctg caactggcgc gtgccggaca gtcattttct tccggtaatt atcaacatgt
    11581 aaaactgctg gaagatgtca ttctcccctc gtatgacgaa ctcagtacca tgctcgacca
    11641 gcgacagcaa accctgctat tgccagagag cgccgccgac atcaccgttc agggtgatgc
    11701 aaccctgctg cggatgttat tgcgaaacct ggtagaaaac gcccatcgtt acagcccaca
    11761 aggcagcaac attatgatta agctgcaaga agatggcgga gcggtcatgg ccgttgaaga
    11821 tgaaggaccg ggtattgatg agagtaaatg cggggagttg agtaaagcgt ttgtacgtat
    11881 ggacagccgt tatggcggga ttggtctggg gttaagtatt gtcagccgca ttacacagtt
    11941 gcatcacggg cagtttttcc tgcaaaaccg gcaagagact tccggcacgc gggcctgggt
    12001 acggctgaag aaagatcagt acgtagcaaa ccagatataa agaaagctgc tgaccaccag
    12061 cacactgaac acggtagtta aactttcata aacacggttt ttcattgcac tctcctccgt
    12121 taacctggag gagagtatgc gcgtcagaga ttaagtgaac cttaagagtt caattggcct
    12181 gataagacag cgtcacatca ggccatccgt ttcagcttat tcatcaattc gcggatgttg
    12241 ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc tcgtggtcaa tatcatcgat
    12301 tttctgctcg atattatcgt aatgctcgac gagaatttcc ttcgcttcct gtatatctga
    12361 cgccgccggt gtcgcacctt tcaacggacg atttgccgtc tctttcatgg ttacgccggt
    12421 gattaaacca atcaccgcca ctaccatcag gtaataggca ggcatcatca gattctgcga
    12481 gctttcgacc agccaggccg ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt
    12541 aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga
    12601 agccataacg cccgtaaagc agttaaggat caccgccagc atcagtaacc cggcaaaaat
    12661 caggccgatg acgttactgt taatcagaat aaacgccggg atcgccaaca caaacagggc
    12721 aacactacca agtagcacaa acggacgacg gccaaaacgg tcactcagca agcccatcac
    12781 cggctggaca aacagcatac cgatcataat ggcgataata atcagcaccc cgtggtcttc
    12841 ggagtaatgc aggttatgcg acaaataact cggcatatag gtcagcaaca tgtagtaagt
    12901 cacgttggtg gcaattacca gaccaataca tgtcaacagg ctgcgccagt atttagtggc
    12961 aatctcttta aacgagactt tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag
    13021 tttatcaaca tgctgctgga acgccggagt ttcttccagc gcatggcgca ggtaaagccc
    13081 gataatccct aacggcagag caataaagaa cggaatacgc cagccccagt cgaggaagtt
    13141 cgcttcgccg acaatggtcg aaattaacac caccacgccc gcacccagca caaacccggc
    13201 aatagaaccg aagtccagcc agctgcccat aaagccacgt ttacggtcag gggagtattc
    13261 cgcaacaaat atcgacgccc cggtatattc accgccgacc gagaaacctt gtgccatctt
    13321 acagatcaac agcagaatcg gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc
    13381 aatacagaac gtactgatcg acataatcac aatagtgata gcgaggatct tctggcgacc
    13441 atatttatcg cccaacatac caaagaagag cccgccaagc ggtcgaatca gaaagggaac
    13501 ggagaaagtg gcaagtgcag caaccatctg cacgctgggg tcagcccccg ggaaaaaaac
    13561 tttacctaat gcgtaagcaa caaaaccata aacaccaaaa tcgaaccatt ccattgcatt
    13621 acccagtgat gctgcggtaa tggctttacg cagtttaccg tcatcaataa tggtgacatc
    13681 acgaagggta atcggtttta cttttttcct tttcagcata gctttcctcg cagagttggc
    13741 ccatttccgc aaacgccaga aaaatcagcg cgacggccgc aaaaggggag cgggaattgc
    13801 aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac accctccggt taaggaatgt
    13861 acaatcaaac cagttgacgc ctgaattggg cagttaatga cctttttacc ctaccagtgt
    13921 ttaaatttgt gatcaacttc acacatggat ttcgtttagg actcattgat gtaactgatt
    13981 atttacgcca aatttacaga gaagatttcc tcgaccattg cccccaccaa acgctgcgct
    14041 gaacagagtc gtggcatccc cagcgcgact gtttgccagc gttgggtcac tgaccagcaa
    14101 acgggctgtt tatctgccgt ggaccacttt cccagccaac ttaaaaaatc agcatgctcc
    14161 cctcccgaag aggcaaacca ctgatggtaa tagtgacgcg tggtcggtgc cgcattgata
    14221 ctctctgcca gatgttgtgt cgctttctcg cgcaaatcac ccagtaacaa caccaccgct
    14281 tgcgggcttc ccagacggca ggcatcgcca aatgcacccc agcgcaactc ctccagaacg
    14341 ataaaacagc gcccaggcaa cgaccaggcg ttatacgccc cagcacgcca gcgggtaaaa
    14401 atttgctcgc tctgatcacc tgcctgcacc agtaggcctc gttgcgttaa cgccttttcc
    14461 ttccagcgac aacgggctga aaaccactca tacaaggcgg caatttgctg ggggagctcc
    14521 gggaaagcag agtgctggcg gtgatccatt ttttgcagca gcgccagatg ctgcgcgatc
    14581 tgcgtcagcg ccagatgacc gggatccagc atcgacgcca gtttttccac cattgtcagt
    14641 cgcaggctaa gcggcgcatt taacatgccg ccgttcgtcc atgtctgaag ctgcacacgc
    14701 gaaagaagtt cctgcatcag tcgttcacga aactgctgct gatgggcttg tggaaggcgg
    14761 gcatcatcgc cctgcgccag atccactaaa aagcggggat aaaccgactc cagcacgcga
    14821 ccggggccgt ccagtaacgt cttggtcaat atcgttctgc cgtgaaaagt gtttgaatat
    14881 catcgcgtaa cagctgggcg tcggtgtaaa tccagccgtg agtcatcaca gtctgctgca
    14941 attgctgctg catcagcctg accaccgatt cattttgttg acgcagagcc aggctttcgc
    15001 gtaaacgcgt ctgtaattcc gtcaaacatg aagcgaactc agcgcaaaaa gtattcatgc
    15061 ctgccgtaac agattcatcg acctgctctg ccagaacttt agccatttgt tggcaataaa
    15121 gatcgacttc tgcgcttaaa gctcgttgca acacactgta atcaaccgtc tctgtcgggg
    15181 atttctcatt tccccgtccc cagtcgggct gattcaacca gcgcgaaaaa gtctcacgca
    15241 caacgcctaa acgcgtgctc tgctcgtccg ttgcatcctg gcgcgaaatg acttcactga
    15301 acagctggcg agtgttgacg tggggaacta ctccgtgaaa aacaggaaaa tgaaccccag
    15361 gacgaaaccc tgactcgctc aattccattt tgacttgttg ctcaatgggc cgaataacat
    15421 cggttaacac ccggcaaagg gtggattcaa gctcggcaaa acgcagcgta aagtcgcgac
    15481 tgatggtgtt ctgcgccgtc tgtaacagtg tctcacagca ggtacgaatc tcgcttaacg
    15541 gctccgaatc atcctgaaac aaggcggcta actgcgcatt cagcgcatct tgctgttgac
    15601 gcagaaagtg gttggcggag gtcagggcca gctcgatttc atgtttaatc tcgccgctca
    15661 cctgcgcctg attgagttgc aatagctgca aactttcttc gacctgatgg atattttgcc
    15721 gcaattgttc acaagcgacg tttaacccgt gcgcacgaaa atccaggtat tcccgcgcct
    15781 gctgcgcgta attcaacagt ttatgcgcag cagatcgcag agcatacaac gaggcgttag
    15841 cgtaagcggc atgaagcaac gcctgaattg gctgggcgaa cagcgaatct tcccacaact
    15901 gatcggcagc atgacgaata tgttcgaggt ccgccagatc ggcatgacgc cagcgcctgc
    15961 cgagcgcggc atgggcaaaa tcttccaccc agcgttgttg ctctggcgct ggtaacttac
    16021 cgttgttggc taactcatgg cgcgctcgat ttgccaagta gccccacatc gacgacaccg
    16081 gaaatatctg ctgtggcgta atacagcctt tcatcagcgt cccggaaatc agtgcccgca
    16141 cctggtcggc gtcgtcactg ttacgatcct gttgatcgaa cttattgacc agcacataca
    16201 gtggcaccga ttgccccacc gccaaaatcg cctcacggac ctcttcatcg gagatcgatt
    16261 tcagttgcgt ataatccagc accgccagta ccgccgaggc gcgtgccagc tgctggttaa
    16321 gcattttttg cagatgcggt tgcccggctt catttggccc gggggtatcc agtaacgtca
    16381 actgaccggg ataactctcc agccccgcca gatggacaaa ctccacttca atcacgggaa
    16441 tatgctcaat ggcggcgtaa gcagaaaaag gaaaatcgac gtccagcgcc ttcgccagtc
    16501 gcactaaatc attcaaactt ttcagacaat gaaaaatagg ctgggcaccc agataatatt
    16561 tttcgaaagc gacgccattt tcgatccgct gcataagcgc acgcatatct ttatctattt
    16621 ccagcacatc ggtcagatgc ttaatatcgc aatcacgcag gcgctgttgt aattgttgaa
    16681 ttaaacaatc gattggcgcg acatgtgaaa aatgcagtac cggttccttt tgcccgggcg
    16741 tatggcgaat aagcgtcggc agcgcagtca ttgggcgatt acgattaggc agaacctccg
    16801 taccaacaat ggcattaatg gtggttgatt tccctgcttt catagtaccg acaatcgcca
    16861 gcaccatttc cagtcgggaa attttactca actcattatt cagcatcgcg tgacgttcgg
    16921 cgatattagg ctgactccag ggtaaagcca gttgtggcgc gtcgtctccg ggtacagaga
    16981 gaggcatttt ttccagtaac tgcaactgtt ggcgagaaag ctgtaacagg cgttcagcct
    17041 cctgacttaa ctcatacagg gtctgtgtgt acatagaaaa ttcttcctta aagcaaattt
    17101 tgttactttt atttagccag attgtttttg agttctgttt gagttctgtt tttggctttt
    17161 ataattactg caagaaataa ttttatattt agtgtgttgt tttttatcag aataaataac
    17221 gtcttctgat gcgtttaaaa cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa
    17281 caagtagagc attaacatta tcttaaataa taaatagagg caaaaagatt attttctttt
    17341 tgcgtttcct ttcaaatgaa aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact
    17401 cagggatttt gtgtataatt gcggcctttt tcggcaatct gccgtttttt ggcgcttttg
    17461 ccctgctgac ttttgaggaa atccacatgt cattaccaca ctgcccaaaa tgcaactccg
    17521 aatacactta cgaagataac ggcatgtaca tctgcccgga atgtgcctac gaatggaacg
    17581 acgcagaacc tgcacaggaa agcgacgagc tgatcgttaa agatgctaac ggcaatctgc
    17641 tggctgacgg cgacagcgtt accatcatta aagatctgaa ggtgaaaggt agctcttcga
    17701 tgctgaaaat tggcaccaaa gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg
    17761 attgcaaaat cgacggtttt ggtccgatga aactgaaatc tgagtttgtg aaaaagaact
    17821 gattgtattg tgatcggtaa gccggataag gcgctcgcgc cgcatccggc aacgatgccg
    17881 actgcctgat gcgacgcttg cgcgtcttat caggcagcct ctcatctgta taaatttcga
    17941 actacactta attggcttct cttaactgag gtcaccatca tgccgttaag tccctacctc
    18001 tcttttgccg gtaactgtgc cgacgcaatt gcttattatc aacgtacgtt gggcgctgaa
    18061 ctgctctata aaatcagctt cggcgaaatg ccaaaatcag cgcaggacag cgccgagaac
    18121 tgcccttccg gaatgcaatt tcccgatacc gccatcgccc acgccaatgt gcgcattgcc
    18181 ggaagcgaca tcatgatgag cgatgccatg ccgtcaggaa aagccagcta ctccggcttt
    18241 acgctggtgc tcgattcgca acaggtcgaa gaaggaaaac gctggtttga caatcttgcc
    18301 gctaacggaa aaatcgaaat ggcctggcag gaaactttct aggcgcatgg ctttggcaaa
    18361 gtcaccgata aatttggcgt accgtggatg attaatgtcg tcaaacaaca accaacgcaa
    18421 taacccgccg ggaggcccgc cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct
    18481 gttagtcact tttaattaac caaatcgtca caataatccg ccacgatgga gccacttttt
    18541 tagggaggct gcatcatgca aacgattatc cgtgtcgaga agctcgccaa aaccttcaat
    18601 cagcatcagg cgctgcatgc ggttgctctg aacattcatc acggtgaaat ggtggctctg
    18661 cttgggccgt cgggttccgg caaatccacc cttttacgtc acttaagcgg tttgattacc
    18721 ggcgataaat ccgccggcag ccatatcgag ctgctgggcc gcacagtcca gcgcgaaggc
    18781 cgtctggcgc gcgatatccg caaaagccgc gccaacaccg gctacatctt ccaacaattc
    18841 aacctggtga accgcctgag cgtactggag aacgtgctga ttggcgcgct cggcagcacg
    18901 ccgttctggc gcacctgttt tagctggttt acccgcgagc agaaacaacg cgcgttacag
    18961 gcgctgaccc gcgttggcat ggtgcatttt gcccatcaac gcgtttccac cctctccggc
    19021 ggacagcagc agcgtgtggc gattgcccgc gcgctgatgc agcaggcgaa ggtgattctg
    19081 gccgatgaac ccatcgcctc gctggacccg gaatccgccc gcatcgtgat ggacaccctg
    19141 cgcgacatca atcagaacga cggcatcacc gtggtcgtca cgctgcatca ggtggattac
    19201 gccctgcgct actgcgaacg catcgtcgcc ctgcgccagg ggcacgtttt ctacgacggc
    19261 agcagccaac agtttgataa cgaacgtttt gaccatctct accgcagcat taatcgcatc
    19321 gaagagaacg cgaaagctgc ctgacatccc catcattgag gaaaacgaat gaacgctaag
    19381 ataattgcct cgctggcctt caccagcatg ttcagcctca gcaccctgtt aagcccggca
    19441 cacgccgaag agcaggaaaa ggcgctgaat ttcggcatta tttcaacgga atcacagcaa
    19501 aacctgaaac cgcaatggac gccattctta caggatatgg agaagaagct gggcgtgaag
    19561 gtgaacgcct tctttgcccc agactacgca ggcattatcc agggaatgcg cttcaataaa
    19621 gtggatatcg cctggtacgg caacctgtcg gcaatggaag cggtggatcg cgccaacggc
    19681 caggtcttcg cccagacggt cgcggcggat ggatcgccag gttactggag cgtgttgatc
    19741 gtcaacaaag atagtccgat caacaacctg aacgatctgc tggcgaagcg gaaagatctc
    19801 accttcggca atggcgatcc taactccacc tctggcttcc tcgtccccgg ttactacgtc
    19861 tttgccaaaa acaatatctc cgccagcgac tttaagcgca ccgtcaacgc cgggcatgaa
    19921 accaacgcgc tggccgtcgc caacaagcag gtggatgttg ccaccaacaa caccgaaaac
    19981 ctcgacaagc tgaaaacctc cgcgccagag aagctgaaag aactgaaggt gatctggaag
    20041 tcgccgctga tcccaggcga tccgatcgtc tggcgcaaga atctctccga aaccaccaaa
    20101 gacaagatct acgacttctt tatgaactac ggaaaaacgc cggaagagaa agcggtgctg
    20161 gaacgcctgg gctgggcacc gtttcgcgcc tccagcgacc tgcaactggt gccgattcgc
    20221 cagctcgcac tgtttaaaga gattcagggc gtaaaaagca ataacggact gaatgagcag
    20281 gacaagctgg cgaaaaccac cgagattcag gcgcagctgg atgacctgga ccgcctgaac
    20341 aacgcgttaa gcgcgatgag ttcggtgagt aaagcggtgc agtaatgtgc tcgatcggtc
    20401 ccctctccct tgagggagag ggttagggtg agggggaaat gcgcacgctg atgccctcac
    20461 cccggccctc tcccacaggg agagggagaa aacattaaac ggagccaaac atgcaaacca
    20521 tcaccatcgc cccacccaag cgcagctggt tctcgcttct gagctgggcc attgttctcg
    20581 ccgtgttggt cgtctcgtgg cagggcgcgg aaatggcccc gcttacgctg atcaaagacg
    20641 gcggcaacat ggcgaccttc gccgccgact tcttcccgcc cgatttcagc cagtggcagg
    20701 attacctcac cgaaatggcc gtcacgctgc aaatcgccgt ctggggcacc gcgctggcag
    20761 tggttctctc catccccttc ggcctgatga gcgccgaaaa cctggtgccg tggtgggttt
    20821 accagcccgt tcgccgcctg atggacgcct gccgcgccat taacgaaatg gtcttcgcca
    20881 tgctgttcgt ggtcgccgtc ggtctcggac cgttcgctgg cgtgctggcg ctatttattc
    20941 acaccaccgg cgtgctctcg aaactgcttt ccgaagcggt ggaagcgatt gaacccggcc
    21001 cggtggaagg cattcgcgcc accggcgcca acaagctgga agagatcctt tacggcgtgc
    21061 tgccacaggt aatgccgctg ctgatctcct actccctcta tcgcttcgaa tccaacgtcc
    21121 gctcggcgac cgtcgtcggc atggtcggtg caggcgggat cggcgtcacc ctgtgggaag
    21181 cgattcgcgg tttccagttc caacaaacct gcgccctgat ggtgcttatc atcgtcacgg
    21241 tcagcctgct ggatttcctc tctcaacggt tgcgtaagca ctttatctga taagtgaggc
    21301 attgatatct atgcacttgt ctacacatcc gaccagctac ccaacacgct atcaggagat
    21361 agccgcaaaa cttgagcagg agcttcgtca acactatcgc tgcggcgact atcttcccgc
    21421 cgagcagcaa ctggcggcgc gctttgaggt aaaccgccac accctgcgcc gcgccatcga
    21481 ccaactggtg gaaaaaggct gggtacagcg ccgtcagggc atcggcgtgc tggtgctgat
    21541 gcgcccgttc gattacccgc tcaacgccca ggcgcgtttt agccagaacc tgctggacca
    21601 gggcagccat cccaccagcg aaaaactgct gtcggtattg cgccccgcgt ccggtcacgt
    21661 tgctgacgcg ctggggatta ccgagggtga gaacgttatc catttgcgca ccctgcgtcg
    21721 cgtcaacggc gtcgcgctct gtttaatcga ccactacttt gcggacctca ccctctggcc
    21781 gacactgcaa cgtttcgaca gcggttcgct gcacgatttt ctgcgcgagc aaaccggaat
    21841 tgcgctgcgc cgcagccaga cgcggatcag cgcccgccgc gcccaggcca aagagtgcca
    21901 gcgccttgaa atcccgaata tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga
    21961 cggtgaaagc agcccggcgg agtactccgt cagcctgacg cgcgccgaca tgattgaatt
    22021 cactatggag cactgaatgc acgcagatac cgcgacccgc cagcactgga tgtccgtact
    22081 ggcgcacagc caaccggctg aactggcagc ccgcctgaac gcgctgaaca tcaccgccga
    22141 ctatgaagtg atccgcactg ccgaaactgg cctggtgcag attcaggcgc gaatgggcgg
    22201 taccggcgaa cgcttttttg ccggcgacgc cacgctgacc cgtgccgccg tacgcctgac
    22261 tgacggcacc ctcggctaca gctgggtgct ggggcgtgat aaacagcacg ccgaacgctg
    22321 cgcgctgatt gatgcgctga tgcagcaatc tcgccacttt caaaacttat cagaaaccct
    22381 tattgccccg ctggacgctg accgtatggc acgcattgcc gcacgccagg ccgaagtgaa
    22441 cgccagccgg gtcgacttct ttacgatggt tcgcggagac aacgcatgac cctggaaacc
    22501 gcttttatgc ttcccgtgca ggatgcccag cacagttttc gtcgcctgtt aaaggccatg
    22561 agcgagccgg gcgtgattgt cgccctgtat cagctcaaac gcggctggca gccgctgaat
    22621 atcgccacca ccagcgtgct gctgacgctg gccgataacg acacgccggt gtggcttgcc
    22681 acgccattaa gtaacgatat cgtcaatcag agcctgcgtt ttcataccaa cgcgccgctg
    22741 gtcagccagc cggaacaggc gaccttcgcg gtgacggatg aggcgatttc cagcgaacag
    22801 ctcaacgccc tttccaccga caccgccgtt gcgccggaag cgggcgcgac gctgatttta
    22861 caggtcgcca gcctgagcgg cggacgcatg ttgcgtctca ccggcgcggg cattgccgaa
    22921 gaacgaatga tcgctccgca gctgccagag tgcattctgc acgaactcac cgagcgcccg
    22981 cacccgttcc cgctcggcat cgacctaatc ctgacctgcg gcgagcgcct gctggctatc
    23041 ccgcgaacca ctcatgtgga ggtgtgctga tgtacgttgc cgtgaaaggg ggcgaaaagg
    23101 cgatcgacgc cgcccacgcc ctgcaagaga gccgacgccg gggcgatacc gatttgcccg
    23161 aactgagcgt cgcccagatt gaacagcagc ttaacctcgc ggtggatcgc gtgatgacag
    23221 aaggcggcat tgccgaccgc gaactggcgg cgctggcgct gaaacaggcc agcggcgata
    23281 acgtcgaagc gattttcctg ctacgcgcct accgcaccac gctgacgaaa ctggcggtaa
    23341 gcgagccgct cgacaccacg gagatgcgtc tcgaacggcg tatttcagcg gtttataaag
    23401 atattcccgg cggccagctg cttggcccaa cctacgacta cacccatcgc ctgctcgatt
    23461 tcactctgct ggcaaacggc gaagcgccga cgctgaccac cgccgacagc gaacaacagc
    23521 cgtcgccgca cgttttcagc ctgctggcgc gtcaggggct ggcgaagttt gaagaggata
    23581 gcggagcaca gccggatgac atcacccgca cgccgccggt ttacccctgt tcacgttctt
    23641 cccgtttgca gcagttaatg cgcggcgacg aaggctattt gctggcgctg gcttactcca
    23701 cccagcgcgg ttacgggcgc aaccacccgt tcgcgggcga gatccgcagt ggttacatcg
    23761 acgtgtcgat tgtgccggaa gagctgggat ttgcggtaaa cgtcggcgaa ctgctgatga
    23821 ccgagtgtga aatggtcaac ggttttatcg acccgccgga tgagccgccg cacttcacgc
    23881 gcggctacgg gctggtattc ggcatgagcg agcgcaaagc gatggcaatg gcgctggttg
    23941 accgtgcgtt gcaagccccg gaatacggcg agcacgcgac aggcccggcg caggatgaag
    24001 agttcgtgct ggcgcatgcc gacaacgtcg aagccgcagg ctttgtctcg cacctcaaac
    24061 tcccgcacta cgtcgatttc caggccgaac tggagctact caaacgtctg caacaggagc
    24121 agaaccatgg ctaatctgag cggctacaac tttgcctacc tcgacgagca gaccaaacgc
    24181 atgatccgcc gcgccatctt aaaagcggtg gcgatccccg gttatcaggt accgtttggc
    24241 gggcgcgaga tgccgatgcc gtacggctgg ggaactggtg gtatccagct caccgccagc
    24301 gtgattggcg aaagcgacgt gttgaaggtg attgaccagg gtgcggatga caccaccaac
    24361 gccgtgtcga ttcgcaactt ctttaagcgc gtgaccgggg taaacaccac tgaatgtacg
    24421 gacgatgcga cgcttatcca gacgcgtcac cgcatccccg aaacgccgct gaccgaagat
    24481 cagatcatta tcttccaggt gccaatcccg gaaccgctgc gctttatcga gccgcgcgaa
    24541 acggagaccc gcactatgca cgcgctggag gagtacggcg tgatgcaggt gaaactgtat
    24601 gaagatatcg cccgcttcgg tcatatcgcc accacctatg cttatccggt gaaggtgaac
    24661 gggcgctacg tgatggaccc gtcgccgatc ccgaaattcg ataacccgaa aatggacatg
    24721 atgcccgccc tgcagctgtt cggcgcaggg cgcgagaagc gcatctatgc ggtgccgccg
    24781 tttacccgcg tggaaagtct cgatttcgac gatcacccgt tcaccgttca gcagtgggat
    24841 gagccatgcg ccatctgcgg atcgacccac agctatcttg atgaagtggt gctggatgac
    24901 gccggaaacc gcatgtttgt ctgctccgat accgattatt gccgccaaca gagcgaggcg
    24961 aaaagccaat gaatcaaccg ttactttcgg tcaataacct gacccacctt tacgcgccgg
    25021 gcaaaggctt tagcgatgtc tcttttgatt tatggccggg ggaagtgctg ggcattgtcg
    25081 gggaatccgg ctccgggaag accacgctgc tgaagtcgat ctccgcgcgt ctgacgccgc
    25141 agcaggggga gattcactat gaaaaccgtt ccctctatgc aatgagcgag gccgaccgcc
    25201 gtcgcctgct gcgcaccgaa tggggcgtgg tgcatcagca tccgctcgac ggcctgcgcc
    25261 gccaggtgtc ggcaggcggc aatatcggcg agcggctgat ggcgaccggg gcgcgacatt
    25321 acggcgatat tcgcgctacc gcgcagaagt ggctggaaga ggtggagatt cccgccaacc
    25381 gtatcgacga cctgccgacc accttttccg gcggtatgca gcagcgtttg cagattgccc
    25441 gcaacctggt gacgcatccg aagctggtgt ttatggatga accgaccggc gggctggatg
    25501 tgtcggtgca ggcccgcctg ctcgacctgc tgcgcggact ggtggtggag ctgaacctcg
    25561 cggtagtgat tgtcacccac gatttaggcg tcgcccgcct gctggcggac cgtttgctgg
    25621 tgatgaagca ggggcaagtg gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc
    25681 atcatccgta tacacagctg ctggtgtcat cggttttgca gaattgagcc ggtgccggat
    25741 gcggcgtaaa cgccttatcc ggcctacaaa tgcgctcccc gtaggtcgga taagacgcgt
    25801 cagcgtcgca tccgacaccc gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag
    25861 taaaaccttc atcctgcacc agcaaaacgg cgtgcgcctg cccgtcctca accgcgcctc
    25921 gctcatcgtc aacgcgggcg aatgcgtggt gctccacggc cattccggca gcggaaaatc
    25981 aaccctgcta cgctcgctgt acgccaacta tctgcccgac gaagggcaaa tccagatcaa
    26041 acacggtgac gaatgggtcg atctggtcac cgcgccagcg cgcaaagtgg tggagatccg
    26101 caaaaccacc gttggctggg tgagccagtt tctgcgcgtc atcccgcgta tttcggcgct
    26161 ggaagtggtg atgcagccgc tactcgatac cggcgtaccg cgtgaagcct gcgccgctaa
    26221 agccgcgcgt ctgctcaccc gcctgaacgt gccggaacgc ctgtggcatc tggcgccatc
    26281 gaccttctcc ggcggcgaac agcagcgcgt caacatcgcc cgcggcttta tcgtcgacta
    26341 ccccattctg ctgctcgacg aacctaccgc ctcgctggac gccaaaaaca gcgccgccgt
    26401 agtcgagctg attcacgaag ccaaagcccg tggcgcggcc atcgtcggca tcttccacga
    26461 cgaagccgta cgtaatgacg ttgccgaccg cctgcaccca atggaagcct cttcatgatt
    26521 atcaataacg tcaaattggt gctggaaaac gaagtggtac acggttcact ggaggtgcag
    26581 gacggcgaaa tccgcgcctt tgccgaaagc cagagccgcc tgccggaggc gatggacggc
    26641 gaaggcggct ggctgctgcc ggggctgatt gagctgcata ccgataatct ggataaattc
    26701 ttcaccccgc gcccgaaagt tgactggcct gcccactcgg cgatgagcag ccacgacgcg
    26761 ctgatggtgg cgagcggcat caccaccgta ctggatgccg tggcaattgg cgacgtgcgc
    26821 gacggcggcg atcggctggt gaatctggag aagatgatca acgccatcga agagacgcag
    26881 aaacgcggcg tcaaccgcgc cgagcaccgt ctgcatctgc gctgcgaact gccgcatcac
    26941 accacgctgc cgctgtttga aaaactggtg cagcgcgagc cggtgacgct ggtgtcgctg
    27001 atggaccact cgccgggcca gcgccagttc gccaatcgcg agaagtatcg cgaatattat
    27061 cagggcaaat actccctcac tgatgcgcag atgcagcagt acgaagaaga gcaactggcg
    27121 ctcgccgcac gctggtcgca gccgaatcgc gaatccatcg ccgccctgtg ccgcgcgcga
    27181 aaaattgcgc ttgccagcca cgatgacgcc acccacgccc acgttgctga atctcaccag
    27241 cttggcagcg tgatcgccga atttcccacc acgttcgaag cggcggaagc ctcgcgcaag
    27301 catggcatga acgtgctgat gggcgcgccg aatattgtgc gcggcggctc gcactccggc
    27361 aacgtggcgg ccagtgaact ggcgcagctt ggcctgctgg atatcctctc ttccgactac
    27421 taccccgcca gcctgctcga tgcggcattt cgcgtcgccg atgacgagag caaccgcttt
    27481 acgctgccgc aggcggtgaa gctggtgact aaaaatccag cgcaggcgct taatctccag
    27541 gatcgcgggg tgattggcga gggcaaacgc gcggacttgg tgctggcgca tcgcaagggc
    27601 aatcacattc atatcgacca cgtctggcgt cagggtaaaa gggtgttctg atgatgggaa
    27661 aactgatttg gttaatgggg ccgtccggct ccgggaaaga tagcctgctg gcggaactcc
    27721 gcctgcggga acaaactcag ctactggtgg cgcatcgcta catcacgcgc gacgccagcg
    27781 ccggaagcga aaaccatatc gctctgagcg agcaggagtt ttttacccgc gcggggcaga
    27841 acctactggc cttaagctgg cacgctaacg gtctgtatta tggcgtcggc gtcgagatcg
    27901 acctctggct gcacgccgga ttcgacgtgc tggtcaacgg ctcacgcgcc catctgccgc
    27961 aggcgcgggc gcgctatcaa tcggcgctgc tgcccgtctg tttacaggtt tcgccggaga
    28021 tcctccgcca gcgcctggaa aaccgtggcc gtgaaaacgc cagtgaaatt aacgcccgcc
    28081 tggcgcgcgc cgcccgctat actccacagg attgccatac gctcaacaat gacggcagcc
    28141 tgcgccagtc ggtcgacacg ctgctgacgc tgattcatca gaaggagaaa caccatgcct
    28201 gcttgtgagc ttcgcccggc cacgcagtat gataccgacg cggtttacgc gctgatttgt
    28261 gagctaaaac aggcggagtt tgaccaccac gcatttcgcg tgggctttag cgccaatctg
    28321 cgcgacccga acatgcgcta tcatctggcg ctgctggatg gcgaggttgt cggcatgatc
    28381 ggcctgcatt tgcagtttca tctgcatcat gtcaactgga tcggcgaaat tcaggaactg
    28441 gtagtgatgc cgcaggcgcg cggcctgaac gtcggtagca agttgctggc gtgggcagaa
    28501 gaagaagccc gccaggccgg ggccgaaatg accgagcttt cgaccaacgt gaagcgccac
    28561 gacgcgcacc gtttctatct gcgagaaggc tacgagcaga gtcacttccg cttcaccaag
    28621 gcgctgtaac atgagtctga ccctcacgct caccggcacc ggcggcgcac agggcgttcc
    28681 ggcatggggc tgcgagtgcg cggcctgcga ccgagcgcgg cgctcgccgc agtatcgccg
    28741 ccaaccgtgc agcggcgtag tgaagtttaa cgaggcgatc accctgatcg acgccgggct
    28801 gcacgatctc accgatcgct ggtctcccgg atcgttccag cagtttttgc tgacgcatta
    28861 tcatatggat cacgtccagg ggctgtttcc gctgcgctgg ggcgtgggcg atccgatccc
    28921 gatctacggc ccgccggatg aacagggctg cgacgatctg tttaaacatc ccgggctgct
    28981 tgatttcagc cacacggtgg aaccgtttgt ggtgtttgat ttgcaggggt tgcaggtcac
    29041 gccgctgccg cttaaccact caaaactcac tttcggctat ttactggaaa cggcgcacag
    29101 ccgggtggcg tggctgtctg acaccgctgg cttgccggaa aaaacgctga aatttttacg
    29161 caataatcag ccgcaggtaa tggtgatgga ttgcagtcac ccgccgcgcg cggatgcacc
    29221 gcgtaatcac tgtgatttaa ataccgtgct tgcgctgaat caggttatcc gctcgccacg
    29281 ggtgattctg acccatatca gccaccagtt tgatgcgtgg ctgatggaaa acgcactacc
    29341 gtcagggttt gaggtggggt ttgatgggat ggagattggg gtggcgtgat gagagggaat
    29401 gtgcgcgctg gccccctcac cctaaccctc tccccagagg ggcgagggga ccgattgtgc
    29461 tcaatattga atattgcgct cgttttctcc ctctccccat tggggtgagg ggcgatgcct
    29521 gctccatacc caacctcatc gcccatactc atcttccatt ctccgctctt catcctccag
    29581 ttgccgacgc tcctgatcaa gctggcgctg gcgatcgtcc agctgcctgc ggcgatcttc
    29641 aaactggcgg cggcggtcgt catattgtct gcgccgatcg tcgctcactt cacgctgcca
    29701 gccgtcgtcg cgcgaatctt catagtctcg cccacggtca gggttataag cgtcattaat
    29761 cgcctgctga atattaccaa tagtatcgtc gataatatca gcctgggccg gaacggtgga
    29821 cagcgtgagc agggtgaata aaagaaatag cggaaagcgt ttcattagcc aacctcaaaa
    29881 agaaactcta tccacattag tcattactca tccatgcaag tagtggatga atctcaattt
    29941 ctccgctgct ctattgccgt aatcgcctcc acgcgttgtt gatgacgacc gccttcgtac
    30001 tgtgcgccca gccacgcatc cacaatcatt tttgccagtt cgaggccaac cactcgtgaa
    30061 ccaaaagcca gtgcaacttc actggcataa tgcggataat cagtgcgctc tgacgacctg
    30121 gtgtctttat caatcacctc actcattgac catgaataaa aggctggcac agcaacgcag
    30181 aatcctgcgg tgccagcgac atgaccgccc attgctccgg tacgtttttt aaaaagaaac
    30241 accggttgca ttgataaatt gtgctcagta tttgccgaag tacaatatat ataataaata
    30301 tataaaacac ctcatgttat ttacagagat aataaattcg cgtaattttc cctcttcagc
    30361 acaaatgcct aataaaatgg atcgttcagc tcacacttcc acttattaaa gtcgcaaatt
    30421 cccaattatg ataaccccag ctttaaatga cgtattttgc atggattaca tatttacgac
    30481 ataaatgata aatggagatt tacatgaata actctcggtt attccgtttg agcagaattg
    30541 ttattgcgtt aactgccgcc agcggcatga tggtaaatac cgctaacgcg aaagaggaag
    30601 cgaaagccgc cactcaatat acccaacagg ttaatcagaa ttacgccaaa tcattaccgt
    30661 ttagcgatcg tcaggatttt gacgacgctc agcgtggatt tatcgctccg ctgctggatg
    30721 aaggtattct gcgcgatgct aatggcaaac catattatcg cggggaagat tataaatttg
    30781 atatcaatgc gcccgcgccc gaaaccgtta accctagcct gtggcgtcag tcgcagttaa
    30841 acggtatttc cggcctgttt aaagtcaccg acagaatgta ccaggttcgc ggtcaggata
    30901 tctccaacat cacctttatc gaaggtgata ccgggattat tgtcatcgac ccgctggtga
    30961 cgccaccgag cgcaaaagcc gcccttgacc tttacttcca aaatcgcccg caaaaaccga
    31021 ttgttgcggt tatttatacc cacagccata ccgaccacta tggcggcgtg aaaggcatta
    31081 tctctgaagc tgatgtgaaa tccggtaaag tgcaggttat cgcccctgct ggctttatgg
    31141 acgaagccat cagcgaaaac gtactggcgg gtaatattat gagccgccgt gcactttact
    31201 cctacggcct gctgctggcg cataaccctc agggtaacat cggcaatggt cttggcgtaa
    31261 cgctggcatc gggctacccg agcatcatcg caccgaacaa aaccatcacc aaaaccggtg
    31321 agaagatgat tatcgacggc ctggagtttg acttcctgat gaccccaggt agcgaagcac
    31381 cagccgaaat gcacttctat attccggccc tgaaagcgct gtgtaccgcc gagaacgcca
    31441 cgcataccct gcacaacttc tacactctgc gcggcgcgaa aacccgcgac accagcaagt
    31501 ggaccgagta tctgaacgaa acgctggata tgtggggtaa cgacgcggaa gtcctgttta
    31561 tgccgcacac ctggccggtc tggggcaata agcatatcaa tgattatatt ggtaaatatc
    31621 gcgatactat caagtacatt cacgaccaga ccctgcacct ggcgaaccag ggctacacca
    31681 tgaatgaaat cggcgacatg attaaactgc cgcctgcact tgccaataac tgggccagcc
    31741 gtggctatta cggttctgtc agccacaacg cccgcgcggt gtataacttc tatcttggct
    31801 attacgacgg taacccggct aacctgcatc cgtatggtca ggtggagatg ggtaaacgtt
    31861 acgtgcaggc gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa gcgaacaagc
    31921 aaggtgatta ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc aacccgggtg
    31981 accaggtcgc gaagaatctg caagcgaata actttgaaca gctgggctat caggccgagt
    32041 ccgccacctg gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa ggggtgcata
    32101 agttcagcca cggcaccatc ggttccccgg acaccattcg cgggatgtcg gtcgaaatgc
    32161 tgttcgactt tatgtccgtt cgcctcgata gcgcgaaagc cgcgggtaaa aatatcagcc
    32221 tgaacttcaa tatgagcaat ggcgataacc tcaacctgac gctgaacgat agcgtgctta
    32281 actaccgtaa aacactgcaa tcccaagctg acgcctcttt ctacatcagc cgtgaagatc
    32341 tgcacgccgt gctgaccggg caagccaaaa tggcggatct ggtaaaagcg aagaaagcca
    32401 aaattattgg caatggcgcg aaactggaag aaattatcgc ctgtctggat aatttcgatt
    32461 tgtgggtgaa tatcgtaacc ccaaattaat gtcactcgct tttgggggcg ctgaagcccc
    32521 caaaagcatt tattttaaaa atatatacag ccaattaata aataaaatat ataagttatg
    32581 aatgcggtga ctgaataaag ctatttttat tcatcgagaa aatagccata ctgacgcctt
    32641 caatggatat tgatattcac tacagatatt gttcgcagca gacaggatgt atttaatatg
    32701 ccaacagttc tctctcgcat ggcgatgcaa ctcaaaaaaa cagcctggat aattcccgtc
    32761 ttcatggttt cgggatgctc attatctccg gcaatcccgg tgatcggtgc ttattatccc
    32821 agctggtttt tctgcgccat tgccagcctt attttgatgc tcatcacgag gcgagttatt
    32881 cagcgggcaa atatcaatct ggcatttgtc ggaattattt ataccgccct ttttgctctc
    32941 tacgccatgc tgttctggct ggcatttttc taatctattt gagacgcgct catggaaagt
    33001 acgccgaaaa aagctc
//
LOCUS       DAZSBF010000022        32729 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZSBF010000022 DAZSBF010000000
VERSION     DAZSBF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.022"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            159..1817
                     /locus_tag="ACHXWD_000779"
     CDS             159..1817
                     /locus_tag="ACHXWD_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR7619778.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1814..2770)
                     /locus_tag="ACHXWD_000780"
     CDS             complement(1814..2770)
                     /locus_tag="ACHXWD_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR7619779.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2921..4036
                     /gene="macA"
                     /locus_tag="ACHXWD_000781"
     CDS             2921..4036
                     /gene="macA"
                     /locus_tag="ACHXWD_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR7619780.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4033..5979
                     /gene="macB"
                     /locus_tag="ACHXWD_000782"
     CDS             4033..5979
                     /gene="macB"
                     /locus_tag="ACHXWD_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR7619781.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6052..6276)
                     /gene="cspD"
                     /locus_tag="ACHXWD_000783"
     CDS             complement(6052..6276)
                     /gene="cspD"
                     /locus_tag="ACHXWD_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR7619782.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6426..6500
                     /gene="yljB"
                     /locus_tag="ACHXWD_000784"
     CDS             6426..6500
                     /gene="yljB"
                     /locus_tag="ACHXWD_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR7619783.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6599..6919
                     /gene="clpS"
                     /locus_tag="ACHXWD_000785"
     CDS             6599..6919
                     /gene="clpS"
                     /locus_tag="ACHXWD_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR7619784.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6950..9226
                     /gene="clpA"
                     /locus_tag="ACHXWD_000786"
     CDS             6950..9226
                     /gene="clpA"
                     /locus_tag="ACHXWD_000786"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR7619785.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9632..9719)
                     /locus_tag="ACHXWD_000787"
     tRNA            complement(9632..9719)
                     /locus_tag="ACHXWD_000787"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9683..9685),aa:Ser,seq:gga)
     gene            complement(9973..10191)
                     /gene="infA"
                     /locus_tag="ACHXWD_000788"
     CDS             complement(9973..10191)
                     /gene="infA"
                     /locus_tag="ACHXWD_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR7619786.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10476..11180)
                     /gene="aat"
                     /locus_tag="ACHXWD_000789"
     CDS             complement(10476..11180)
                     /gene="aat"
                     /locus_tag="ACHXWD_000789"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR7619787.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11222..12943)
                     /gene="cydC"
                     /locus_tag="ACHXWD_000790"
     CDS             complement(11222..12943)
                     /gene="cydC"
                     /locus_tag="ACHXWD_000790"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR7619788.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12944..14710)
                     /gene="cydD"
                     /locus_tag="ACHXWD_000791"
     CDS             complement(12944..14710)
                     /gene="cydD"
                     /locus_tag="ACHXWD_000791"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR7619789.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14833..15798)
                     /gene="trxB"
                     /locus_tag="ACHXWD_000792"
     CDS             complement(14833..15798)
                     /gene="trxB"
                     /locus_tag="ACHXWD_000792"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR7619790.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16343..16837
                     /gene="lrp"
                     /locus_tag="ACHXWD_000793"
     CDS             16343..16837
                     /gene="lrp"
                     /locus_tag="ACHXWD_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR7619791.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16972..21039
                     /gene="ftsK"
                     /locus_tag="ACHXWD_000794"
     CDS             16972..21039
                     /gene="ftsK"
                     /locus_tag="ACHXWD_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR7619792.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21194..21805
                     /gene="lolA"
                     /locus_tag="ACHXWD_000795"
     CDS             21194..21805
                     /gene="lolA"
                     /locus_tag="ACHXWD_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR7619793.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21816..23159
                     /gene="rarA"
                     /locus_tag="ACHXWD_000796"
                     /gene_synonym="mgsA"
     CDS             21816..23159
                     /gene="rarA"
                     /locus_tag="ACHXWD_000796"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR7619794.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23250..24542
                     /gene="serS"
                     /locus_tag="ACHXWD_000797"
     CDS             23250..24542
                     /gene="serS"
                     /locus_tag="ACHXWD_000797"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR7619795.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24781..27225
                     /gene="dmsA"
                     /locus_tag="ACHXWD_000798"
     CDS             24781..27225
                     /gene="dmsA"
                     /locus_tag="ACHXWD_000798"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR7619796.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27236..27853
                     /gene="dmsB"
                     /locus_tag="ACHXWD_000799"
     CDS             27236..27853
                     /gene="dmsB"
                     /locus_tag="ACHXWD_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR7619797.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27855..28718
                     /gene="dmsC"
                     /locus_tag="ACHXWD_000800"
     CDS             27855..28718
                     /gene="dmsC"
                     /locus_tag="ACHXWD_000800"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR7619798.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28754..29380)
                     /gene="ycaC"
                     /locus_tag="ACHXWD_000801"
     CDS             complement(28754..29380)
                     /gene="ycaC"
                     /locus_tag="ACHXWD_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR7619799.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29695..30843
                     /locus_tag="ACHXWD_000802"
     CDS             29695..30843
                     /locus_tag="ACHXWD_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7619800.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31053..32483
                     /locus_tag="ACHXWD_000803"
                     /pseudo
     CDS             31053..32483
                     /locus_tag="ACHXWD_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(32484..>32729)
                     /locus_tag="ACHXWD_000804"
     CDS             complement(32484..>32729)
                     /locus_tag="ACHXWD_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7619801.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8023 a         8015 c         8794 g         7897 t
ORIGIN      
        1 tgtttttgca gacttatcag caagagggag tataacgcga ttattcgctc atttttcaga
       61 catttgccat gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg
      121 ccagcgtaaa ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa
      181 ttgtgggttt tcgcggtatc aaccgtttgt cgttgatgct ggaacaaaac aacgtcctga
      241 ttggggagaa cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc
      301 cagaatcaga tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca
      361 acgggcgaga acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac
      421 atcgggttcg ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc
      481 accgtatttt ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc
      541 gcagttttct cgataaagac ggacatccga ttgatgtcga ggatattaac gatcaggcac
      601 gccatctggt gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta
      661 ttcgtaacgg cacggtgcca aatgtcccta atatggaagt caccgcgcgc cagctcgatt
      721 tcctcgcccg tgagttatcc tcacatccgc aaaatctctc tgatgggcag attcgtcagg
      781 gactttccgc aatggtacag ctgcttgagc attatttctc tgagcagggg gccggacagg
      841 cgcgatatcg tttaatgcgg cggcgagcca gcaatgagca acgaagctgg cgctatctgg
      901 atatcatcaa ccggatgatt gaccgacctg gtgggcgctc gtatcgggtt attttgctcg
      961 gcctgtttgc tactttgttg caggcaaaag gcacattgcg actggataaa gacgcccgtc
     1021 cattgttgct gatcgaagat ccagaaaccc gtttacaccc cattatgctt tcagttgcct
     1081 ggcatctgtt gaatcttctg ccattgcagc gcattgtcac caccaactcg ggtgagttgc
     1141 tttcgttaac gccggtagag catgtttgcc gactggtacg tgagtcctcg cgcgttgccg
     1201 cctggcgtct ggggccgagt ggcttgagta ccgaagatag ccgacgcata tcctttcaca
     1261 ttcgttttaa ccgtccgtca tcgctgtttg cacgctgctg gttgctggtg gaaggggaaa
     1321 cggaaacctg ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag
     1381 ggatcaaggt cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc
     1441 gaatggggat tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg
     1501 ctacggtacg cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt
     1561 taccggcgct ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc
     1621 gcgtggcgca aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga
     1681 tccatcgctc ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg
     1741 gtgtggactc cgtaccgacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc
     1801 gcggtcgcgc ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca
     1861 tctcatagcg ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc
     1921 gtgctatctg tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgatt
     1981 cccagaaagc gttgtaatca gcgtggatct tgccttcttt atcgcgataa cgcaggctgc
     2041 ggtaaatatg cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa
     2101 acagacaggc cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg
     2161 cattctggat ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca
     2221 tcgttctttt cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt
     2281 cgctgttgcg aaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca
     2341 gctcaagcgt gaactgctcg ccgtttttgc cttccagctt cgccagttgc agcccggggg
     2401 tattcaaata taagctgaat tcttccgccg acatacatcc gcggagtaac gcataatggt
     2461 aacgtaacgc ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca
     2521 gacgcacagg cagtcgcggt tggcgcgtta acaacacatt gagattaggc cagtgggaaa
     2581 gttcgttcat ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct
     2641 ggcgaaaact acggcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa
     2701 gaaaaagtga caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg
     2761 acatattcat gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc
     2821 aaacatcaat agtgtgatta ctaacgtaaa ttttatggtt ttgttgatat ttcgttgaag
     2881 ttaatgaccc ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt
     2941 gaagaagcgt tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag
     3001 aattcttaac gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca
     3061 gcaaagcgtg ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca
     3121 ggtcagcggt cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca
     3181 gcttttaggg gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac
     3241 gctgatggag ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt
     3301 gacgtattcc cgtcagcaac gtctggcaca aacgcaggcg gtttcacagc aggatctcga
     3361 caccgccgcg acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat
     3421 caagcgcaat caggcttctc tcgatacggc taaaaccaat ctcgattaca ctcgcatcgt
     3481 tgccccgatg gccggggaag tcacgcaaat caccactctg caaggccaga cggtgattgc
     3541 cgcacaacaa gcaccgaaca ttctgacgct ggcagatatg agcaccatgc tggtaaaagc
     3601 gcaggtttct gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt
     3661 acttggcgat ccactgacgc gctacgaggg gcaaatcaag gatgtactac cgacgccgga
     3721 aaaggttaac gacgctattt tctattacgc ccgttttgaa gtccccaacc ccaatggttt
     3781 gctgcggctg gatatgactg cgcaagtgca tattcagctc accgatgtga aaaatgtgct
     3841 gacgatccct ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt
     3901 gttgcgtaat ggcgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga
     3961 tgttgagatt gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc
     4021 aggagctgca caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc
     4081 ggtaatgagc aggttgaggt gctgaagggc atcagcctcg atatttatgc gggtgagatg
     4141 gtcgcgattg ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc
     4201 ctggataagg ccaccagcgg cacctatcgc gtagccggtc aggatgttgc cacgctggac
     4261 gccgatgcgc tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat
     4321 ttgctttcgc atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgctggtctt
     4381 gagcggaaac agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac
     4441 cgtacagagt attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg
     4501 cgggcattga tgaacggcgg tcaggtaatt cttgccgatg aaccaaccgg cgcactggac
     4561 agccattctg gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg
     4621 gtgattatcg tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt
     4681 cgcgacggcg aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg
     4741 acggagcctg ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag
     4801 gcgctgacga tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg
     4861 ctggggatta ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc
     4921 aaacaaatgg tgctggcgga tattcgttct attggtacga atactattga tgtctatccc
     4981 gggaaagatt ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta
     5041 atcgccatcc aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg
     5101 cgcctgcgtt ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat
     5161 tttaatgtct atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac
     5221 ggtcgtgcgc aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa
     5281 gcagatgtgg ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg
     5341 gcggaagaaa aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac
     5401 agcacgatgt ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg
     5461 aaagaaggat ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc
     5521 cacggtaaga aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag
     5581 accacacgta ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggt
     5641 ggtattggtg taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc
     5701 attcgcatgg ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc
     5761 gtactggttt gcctggtcgg tggcgcgttg ggaataactc tgtcactgtt aattgctttc
     5821 accttgcagc ttttcttacc cggctgggag attggttttt caccgttggc actgctgctg
     5881 gcgtttctct gctcgacggc caccgggatt ttatttggat ggttacccgc acgaaatgcg
     5941 gcacgactgg atccagtaga tgctctggca cgagagtaat ttttgagata aaaatgccag
     6001 ccgatcgggc tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac
     6061 tgccgcttct acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg
     6121 gacatcaaac tgaacggatt gtccagcttt tagcgttcta taaccatcca tctgaatggt
     6181 ggaataatga gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt
     6241 ggcattgttg aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt
     6301 atacaagtaa gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact
     6361 ctagaaatac aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg
     6421 caggtatgaa tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg
     6481 ctgatgccaa cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa
     6541 gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat
     6601 gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc
     6661 gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga
     6721 gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt
     6781 gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc
     6841 agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg
     6901 tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca
     6961 agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt
     7021 tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct
     7081 ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca
     7141 aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt
     7201 tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac
     7261 cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg catatctgtt
     7321 gcgtaaacat gaagtcagcc gtctcgatgt ggtgaatttt atctctcatg gcacgcgtaa
     7381 agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg
     7441 tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc gcgtgggcgg
     7501 aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg ttctctgccg
     7561 tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc
     7621 ggaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg ctgactgtac
     7681 gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga
     7741 aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat
     7801 tgatgagatc cacaccatta tcggtgcggg ggcagcgtct ggtggtcagg ttgatgcggc
     7861 taacctaatc aaaccgttgc tctccagcgg taaaattcgt gtaattggtt cgacaaccta
     7921 tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat
     7981 tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa
     8041 gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct
     8101 ggcggtgaaa tacatcaatg atcgtcatct gccggataaa gcgattgatg ttatcgacga
     8161 agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc
     8221 ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag
     8281 tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggtcagga
     8341 taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga
     8401 acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt
     8461 gacggtacag ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta
     8521 tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga
     8581 tcagggcggt ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga
     8641 cgaaatcgag aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg
     8701 tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac
     8761 caccaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa
     8821 cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct
     8881 cgacaacatt atctggtttg atcatctgtc aaccgacgtg atccatcagg tggtggataa
     8941 attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca
     9001 ggaagcgcgt aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg ctcgtccgat
     9061 ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc
     9121 gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg agctgactta
     9181 cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatcta attgttaggt
     9241 aggttggtga agtccgtaat ctcgaaagag gttacggact ttttgtttat ggggtggaac
     9301 ggtgaaaacc ctatttttgg aggtgaaggt aatttgttga taattagtgc tgctggaaaa
     9361 ggtaaggata aaaaagggtg acagcaggaa aatgagatgg ttttgcttta ttaacaacgg
     9421 actaaacgtg tagtatttga gttcactgcc gtacaggcag cttagaaatc gacggggtgc
     9481 ggtaaaacct ttgcgaacgc gttcactgcc gtacaggcag cttagaaatt cacaggtaac
     9541 atactccacc cgcccaccat gttcactgcc gtacagaaag ataaaatgcg aaaaaaaagc
     9601 tcgcactttc gtacgagctc ttctttaaat atggcggtga gggggggatt cgaacccccg
     9661 atacgttgcc gtatacacac tttccaggcg tgctccttca gccactcgga cacctcacca
     9721 aattgttttg ctaccaaacc tcatgggtgg caacggggcg ctactatagg gagttggagt
     9781 aaaacggtca agaagaattt taatgataat tattgtttgc tcatactgta aacaagttgt
     9841 gcagtatatc tacatcgaga caagttacgg acttatactt ccaaagtact tcatacatat
     9901 cacaaaataa aaaggccggt taaaccgacc ttttactcgt tctttctctt cgcccatcag
     9961 gcggtaaaac aatcagcgac tacggaagac aatgcggcct ttgctcaggt cgtacggggt
    10021 cagttcaaca gtcactttgt cgcccgtcag gatgcggatg tagtttttgc gcattttacc
    10081 ggagatgtgt gcagtaacca cgtgaccgtt ttctaactct acgcggaaca tggtattagg
    10141 caacgtttca agaacggtac cttgcatttc aatattgtct tctttggcca tctaatcctc
    10201 tggggtatca ctaccgtaat ttgaaccggc aagataatgc cgaagttctg taaataagta
    10261 aagatttgcg cgctaaatcg caacaaacag gttcggcaca ttactccgaa aacacacggc
    10321 taagccgcac caaaagcgca acgtataagg gagcggtgag ataaacgatg ggcgttacct
    10381 gacgcgaaaa attccttatc ggcagcgggg taatgagcat aaccaactct gcgaccgcaa
    10441 ttataacact ctggggagaa atgtgccgaa aacattcatt tttgtggtga aaacaagcat
    10501 cgtggcaccc agaaattatt cggcaatcgt ccgaggcgca tttgattgag ataattcaga
    10561 taatcccgac ggggaatttc gcaggcacca agcgatgctg tgtgatcgtt aaggacctgg
    10621 cagtcgataa gcttaccgcc atgactgata aattcctcac agaataccag aagcgccgtt
    10681 ttagacgcat tttccatccg gctgaacatg gactcgccac aaaatagcgt tccctgggcc
    10741 acgccgtaca taccgccgac aagctcatct tcatgccaga cttcaatgga gtgggcatga
    10801 ccgagttcgt gaaggcgatg gtaggcttcg accacgccac gcgtgatcca ggttccttct
    10861 tcgcgatcgc tggcacaacc ttcaatgacc tgaccaaaag cgtaattcat cgtgacacga
    10921 tagggcgagc gtttatgaaa tcgcttcata ctacggctga tatgcagtga ttcaggccaa
    10981 agcaccgcgc gaggatcggg cgaccaccag aggatggggg cgcctggaga aaaccacgga
    11041 aaaataccac gctggtaagc cattaacagg cgcgcagggc taagatcgcc ccccagagcc
    11101 agcaggccgt taggctcacg taatgcgcct tccggggaag ggaaggctat tgaatggcga
    11161 gaaagctgaa ccaggcgcat gaccgcaaaa ctccacgcaa gtcggatcgt tcaataatag
    11221 cttacaaacc ctgcttaaac tggtaataac gcccctgtct ggcaagcagt tctgcgtgag
    11281 taccttgctc aataatttgc ccgttgtcca tcactattat ttgttggaaa cgagagagtc
    11341 cgcgaagtcg atgggtgacc attaacaccg ttttctcacg catcatttct gcaagcaatt
    11401 caaggatctg gctttcggtt gtggcatcta agccttcggt aggttcatcc agcaacacca
    11461 gtggcgcatc atgtaacagc gcacgggcga tagccagacg gcgcagttca ccaccggaga
    11521 gctggcgtcc gccttcacct aaccaactgt tgagacctgc gtcctcgagc agcttctcca
    11581 gaccaacgcg acgcaagatc tccgacagag cctcatcact actgccaggc gaggcgagta
    11641 acagattatc acgcagcgtg gcgctaaaca gatgcactcg ctgaggaaca acgctgatgg
    11701 tctgtcgtag agccgcttca ttcaggctgg ctatggggct atcgttaagc aaaatctcgc
    11761 cctgttgcgg gtcccaagcg cgggtcagca gttgtaacag tgttgatttg ccgcatccgg
    11821 ttcgcccgag aatcgcaata tgttccccgg cgtttacctg aagagaaatc cctttaagtg
    11881 cctgttggga ttgctccgga taagtgaact gaacatcccg taatgtcagc gaaacgcgat
    11941 cggcaacacg agtttgggta tcagggaagg tgacttccgg tttttgatcc gttaaatcag
    12001 tgatccgaac ggcagaggca atgacttgcc ccagatgctg aaatgcaccc gttactggtg
    12061 ccagtgcttc aaacgcggct aacgcgcaga agacaaacag ggcaattaac gcgccgggtt
    12121 gagcattgcc gccaacgccg ccagacgcca tccacagcat caggatcacc gctaacgcgc
    12181 caatgagcag cattatcgct tgcgacaatg cggtcagttc agattgacgg cgttgcgctt
    12241 ccagccattg aatttctgta ttctctagtt gcgtgcgata acgatcgctg gcaccaaaaa
    12301 tggtcagctc agcttgccct tgcagccagg ccgtcagttg ttggcgatac tgtccgcgaa
    12361 gatgagtcag attttgcccg gtgctttttc ccgcacgata aaacagcggt ggcatcagga
    12421 aaagcgtcag taacataatg ccgcccagcg taaaggcgag ggtgaaatca aggaaactta
    12481 acccgactgt caccaccata atcaccacaa aagcgcccac cagcggcgag ataacgcgca
    12541 ggtaaagatg atcgagcgta tcaacatccg ccaccacgcg attgagcaat tcgccctgac
    12601 gatagcgcgc cagtccggca ggggagaggg gcaacaattt gctgaaggtg taaatgcgca
    12661 gatgctgcaa cacgcggaaa gtcgcgtcgt gacttaccag acgttcaaaa taacgcccgg
    12721 cagtacgggt gattgcggca ccacgcacgc ccgcagcggg tagcatatag ttgaagctgt
    12781 acagtccggc accccccgca accgctgagg ccgagaggaa ccagccggaa agtgtcaaca
    12841 gaccgatact ggcgagcagc gtcacaattg ccagcacaat accaagactt aacatccatt
    12901 tatgacgttt atacagtgcc agatagggta gcaaagcgcg catttaaatc tcctcctgac
    12961 gatgggccag taatgtggcg aatgggccac cagccacact taattccgcg taacgtcctt
    13021 gctcaataat ctgaccatcc tgcattaccc aaatgacatc ccagtcagca agatcttcta
    13081 actggtgggt gaccattaac gttgtctggc gcagagaggc ggcattcagc gcctccatta
    13141 cgcgctgttc actgtgagca tcaaggctgg cagcgggttc atccaacagt aatagcgaac
    13201 agggatttag taacgcacgg gccaccgcca cgcgctgcgc ctgccccacg gaaaggcggg
    13261 cagcctggtc gccaacaggc gtatcaacgc cttgtggcag gagcggtaga aactcgctga
    13321 cccaggcgtt atccagcgct gcttgtaatt catgttcgct ggcatcaggt cgcgccagta
    13381 gtacgttatc ccgtaatgtt gctgccggta attgtggatt ttgcccaacc caggagagat
    13441 gtttacgcca tgattctggt gataaatcgc gtaattctat cccgttgatt cgtagcgatc
    13501 cctgatatga gagaaaacca gaaagcgcgt tcaacagtga acttttacct gaaccgctgc
    13561 gaccaaccaa cacggcacgt tggcctgctg gcaaagtaaa gttcagcggt ccggccagcg
    13621 ttttaccttc cggcgacgtg ataaacagat cctcggcctc aatggtcacc ggatcggtcg
    13681 atgctaattc cacctcgcca cgctgcggat gggcgagcgg ggtttccata aacgttttca
    13741 gactgtcagc tgcaccaaca gcctgggctt tagcatgata aaacgtaccg agatcgcgta
    13801 atggctggaa aaactctggc gcaaggatca gggccagaaa acccgcagcc agcgtcacgc
    13861 cagtatcgta gtgaccaaaa tccagctcgc cgagatagga aaaaccaaag tagaccgcca
    13921 ccagagcaat tgacagcgag gtaaaaaatt cgagaatgcc ggaggataaa aatgccagcc
    13981 gtagcacttc cattgtccgt tggcggaaat cttccgaagc agaacgaata ctttcaattt
    14041 cagcttcacc acgaccaaaa atacgcaatg tttccatgcc gcgcaggcga tcgaggaaat
    14101 gcccacttaa gcgagcaaga gcgagaaagt tacgtcggtt agcatcggca gcccccattc
    14161 caaccagcgc cataaacaac ggaattaacg gtgcagtgcc cagcagaatg agcgccgcag
    14221 cccagtttga ggggaagatt gccaccacaa tcagcaacgg caccgacact gccagcgcca
    14281 tttgcggcag gtagcgtgca tagtaatcat gcatatcgtc aatttgctcg agtaccagcg
    14341 tcgcccagct ccccgcaggt ttaccctgaa tccacgctgg ccctgcttgt tgcagacggt
    14401 cgagaacctg acggcggatg gcaaagcgga tatgctgccc ggcgtgataa cccacccgtt
    14461 cgcgtaacca gaccgcccat gcgcgcagta caaaggtcag aaccagtaac gtaaagggaa
    14521 gcagcagggc ttcacgggga atattctcca taatcatatg ttgcagaatt cgcgccatga
    14581 accaggcctg ggcaatgatc aatatgccgc ttacaaagcc cagcagacga gaaatattca
    14641 gccaacgttg ggagatgacg ctttgctgtt ttaaccagcg ggttaactct ttttgacgag
    14701 atttattcat tgcacgctta gcaggtgagt tatcagaatt atttgcagag caatgttaca
    14761 acggggaaaa aataaaggcg acccatagtc gcaaggtgtc gccttcttta cttttgttac
    14821 tgatttgtaa aattattttg cgtcagctaa accatcgagg tagcgttccg catcaagtgc
    14881 tgccatgcag ccagtaccgg ccgaagtaat ggcctggcga taaatgtgat ccatcacgtc
    14941 gcctgcggca aagacgccag gaatgctggt ctgggtggca ttaccatgaa tacccgactg
    15001 tactttgatg tagccgtttt ccagttccag ctgcccttcg aaaatcgcag tattcgggct
    15061 gtgaccgata gcaacaaaca gaccggcaac gtcgagtgac tcgatgttat cgctgttttg
    15121 cgtatcgcgc agacgaacgc cagtgacacc catttgatcg ccggtcactt cttccagcgt
    15181 acggttggtg tgcagaatga tgttgccgtt ctccacttta tccatcagac gcttaataag
    15241 gattttttcc gcgcggaaac cgtcacggcg gtgaatcaga tgcacttccg aagcgatgtt
    15301 agacagatac agcgcctctt caaccgcggt attgccgccg ccgatgaccg caactttctg
    15361 gttgcgatag aagaaaccgt cgcaggttgc acaagcagaa accccacggc ctttaaaggc
    15421 ttcttcagag ggcaggccga gatagcgtgc agaagctccg gtggcaataa tcagcgcgtc
    15481 gcaagtgtat tcgccgttat cgccattcag acggaacgga cggttttgca gatccacctt
    15541 gttgatatga tcaaaaatga tctcagtttc aaacttggtg gcatgttcgt gcatgcgctc
    15601 cattaataac ggaccggtca gatcgtttgg atcaccaggc cagttttcca cttccgtggt
    15661 ggtggtcagt tggccgcctt tttccatgcc ggtaatcagc acaggttgca ggttggcgcg
    15721 cgccgcgtag acagcagcgg tgtatcccgc cgggcctgaa cccaggataa gcagtttact
    15781 gtgtttggtc gtgcccatga gatccccata gttgttggca gacaatgggc aggattgtag
    15841 ggaatttaca gacgtaaaaa aagagtatga cgattttgtt aacaatttgt gcaatcggca
    15901 gcatcgataa gcaggtcaaa ttctcccgtc attatcacct ctgctactta aatttcccgc
    15961 tttataagcc gattaaatga tgaataaacg cccctgttaa tgaatatctg gcatgttgta
    16021 ctaaaaatcg atgttttgct ttgacaatcc cctggtgttt tgcgaaaaca ttcgaggaag
    16081 aaaaaaaaca gtattcttat atgcgcataa ccatgcatgt aaataccatg tttaccgtgc
    16141 tagtgaaatc tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag
    16201 gcaacggtct tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt
    16261 ctatcgtgac gggtagcgac tctgaacagt gatgtttcag gggcagacag gagtagggaa
    16321 ggaatacaga gagacaataa taatggtaga tagcaagaag cgccttggca aagatctcga
    16381 ccgtatcgat cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga
    16441 gctttctaaa cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga
    16501 aagacaaggg tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc
    16561 acttctggta ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt
    16621 caataccgct gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt
    16681 cgactacctg ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga
    16741 aaccctgctg cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt
    16801 caagcagagt aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc
    16861 gtattttgat tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg
    16921 ttgtccgttt tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag
    16981 gaatacactg aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg
    17041 gaggcgttgc tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc
    17101 tttaaccctt cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta
    17161 ggtgggatgc ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct
    17221 tacaccattc ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc
    17281 gacgaataca ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc
    17341 cttacctcct gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt
    17401 ggcgtcattg gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga
    17461 actattgcgc tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg
    17521 gtgaccattg ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat
    17581 cgtacccgtc gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat
    17641 gaagatgaaa atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg
    17701 ctagcgcgtc gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac
    17761 gctgcgttgt tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc
    17821 ggtgtggctg ccgaccctga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa
    17881 tatgacgaat acgatccatt attaaacggt gcgccaatta ccgaacctgt cgctgtagca
    17941 gctgctgcta ccacggcggc acaaagctgg gctgcgccgg ttgaacctgt gactcagacg
    18001 ccgcctgttg cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta
    18061 ccgggtccac aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag
    18121 ccacaatatg cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagcca
    18181 cagcagccgt attatgcacc tgcagctgaa caacctgtgc aacaacctta ctacgctcct
    18241 gcggcggaac aacctgttca gcaaccgtat tacgcccctg cgccagaaca accggtggca
    18301 ggtaacgcct ggcaagccga agagcagcaa tccacttttg ctccacagtc tacataccag
    18361 actgagcaaa cttatcagca accagccgct caggagccgt tgtaccaaca gccgcaaccc
    18421 gttgaacagc agcctgttgt ggagcctgaa cccgttgtag aagagacaaa acccgcgcgt
    18481 ccgccacttt actattttga agaagtggaa gagaagcgag cccgtgaacg tgaacaactt
    18541 gcggcctggt atcaaccgat tccagaaccg gttaaagaac cagaaccgat caaatcttcg
    18601 ctgaaagcac cttctgttgc agcagtacct ccagtagaaa ccgctgccgc tgtttctccg
    18661 ctggcatctg gcgtgaaaaa agcgacactg gcgacggggg ctgccgcaac cgttgccgcg
    18721 ccagtcttca gtctggcaaa tagcggtgga ccgcgtccgc aggtcaaaga ggggattggc
    18781 ccgcagttgc cacgaccgaa acgtatccgc gtgccaactc gccgtgaatt ggcgtcttac
    18841 ggtattaagc tgccctcaca gcgtgcggcg gaagaaaaag cccgtgaagc ccagcgcaat
    18901 cagtacgatt ctggcgatca gtacaacgat gatgaaatcg atgcgatgca gcaggatgaa
    18961 ctggcacgtc agttcgccca gacacagcag caacgctatg gcgaacagta tcaacatgat
    19021 gtgcccgtaa acgcagaaga tgcagatgct gcggcagagg ctgaactggc tcgtcagttt
    19081 gcgcaaactc aacaacaacg ttattccggc gaacaaccgg ctggggcgaa tccgttctcg
    19141 ctggatgatt ttgaattttc gccaatgaaa gcgttgctgg atgatggtcc acacgaaccg
    19201 ttgtttacgc caattgttga acctgtacag cagccgcaac aaccggttgc accgcagcag
    19261 cagtatcagc agccacaaca gccggttgcg ccacagcagc aatatcagca gccgcaacag
    19321 ccggttgcgc cgcagccaca atatcagcag ccgcaacaac cggttgcgcc acagcagcaa
    19381 tatcagcagc cgcaacaacc agttgcgccg cagccgcagt atcaacagcc gcaacaaccg
    19441 gttgcgccgc agcagcagta tcagcagcca caacagccag ttgcgccaca accgcaggat
    19501 accctgcttc atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg
    19561 acgccgctgc cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat
    19621 acctttgcgc ttgaacaaat ggctcgcctg gtggaagcgc gtctggctga tttccgtatt
    19681 aaagccgatg ttgtcaatta ctctccgggg ccggttatca ctcgctttga attgaacctg
    19741 gcaccgggcg taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt
    19801 tcgacggtgg cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag
    19861 ttaccgaata aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc
    19921 cgcgataatc cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg
    19981 gttgccgatc tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa
    20041 tctgtcggtg tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg
    20101 cgtttcatca tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcat
    20161 ctgttaacgg aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt
    20221 aacgagatgg agcgtcgtta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt
    20281 tataacgaaa aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg
    20341 aagccgggtg acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtg
    20401 gtgttggttg acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg
    20461 atagcacgtc tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag
    20521 cgtccatcgg ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc
    20581 tttaccgtat ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca
    20641 ctgctgggga tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc
    20701 catggtgctt ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt
    20761 ggtcgcccac agtatgttga tggcatcact tccgacagtg aaagcgaagg tggcgcgggc
    20821 ggtttcgatg gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact
    20881 gaaaaacgca aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt
    20941 gcagcgcgta ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac
    21001 ggtaatcgtg aagtgctggc tccaccgccg tttgactaat taatgcattg ccggataagg
    21061 cgcggtagcg tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc
    21121 aagctgatta ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat
    21181 ttgaggaata atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc
    21241 aagcagcgtt tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag
    21301 cttccacgcc agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg
    21361 tcagggcgat ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga
    21421 tgaaagcatt ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca
    21481 agctacggca acttggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg
    21541 caaccagtcc agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct
    21601 gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg
    21661 cacaatccat cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcaactgaa
    21721 atcccagcaa aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt
    21781 cacggtagat gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt
    21841 cggataatac ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata
    21901 tcggccagca acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc
    21961 atctgcattc tatgatcctc tgggggccac cgggtaccgg caaaacaacc ctcgctgaag
    22021 tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga
    22081 aagagattcg cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta
    22141 ttctttttgt tgacgaagtg caccgattca acaaaagcca gcaggatgca tttctgccac
    22201 atattgaaga cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc
    22261 ttaattcggc actgctttcc cgtgcccgtg tctatctgtt gaaatccctg ggtacagagg
    22321 atattgagca agtactaact caggcgatgg aagacaagac ccgtggttat ggtggtcagg
    22381 atattgttct gccagatgaa acacgacgcg ccattgctga actggtgaat ggcgacgcgc
    22441 gccgggcgtt aaatacgctg gaaatgatgg cggatatggc cgaagtcaat gatagcggta
    22501 agcgggtcct gaagcctgaa ttattgaccg aaatcgccgg tgagcgaagc gcccgctttg
    22561 ataacaaagg cgatcgcttt tacgatctca tttccgcact gcataagtcg gtacgtggta
    22621 gcgcacccga tgcggcgctc tactggtatg cgcgaattat taccgctggt ggcgatccgt
    22681 tatatgtcgc gcgtcgttgc ctggcaattg cttccgaaga tgtcggcaat gccgacccac
    22741 gggcgatgca ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag
    22801 gtgaacgcgc cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg
    22861 tctacactgc gtttaaagct gcgctggcgg atgctcgcga acgcccggat tatgacgtgc
    22921 cggttcattt gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat
    22981 atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg
    23041 aaatagcaca aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg
    23101 aaaagctcgc ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat
    23161 gttatcgttg cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc
    23221 ccatttaatt cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc
    23281 cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg
    23341 gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc
    23401 gtaactcccg atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt
    23461 tacgtctgga agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg
    23521 ctttacaggc tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag
    23581 tgccggtagg taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg
    23641 agtttgactt tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact
    23701 ttgcagctgc agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc
    23761 gcatgcaccg cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca
    23821 gtgagaacta tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc
    23881 cgaaatttgc tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta
    23941 actatgcgct gatcccaacg gcagaagttc cgctgactaa cctggtacgc ggtgaaatca
    24001 tcgatgaaga tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag
    24061 ccggttcata tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg
    24121 aaatggtgca gatcgtgcgc ccggaagact caatggcggc gctggaagag atgactggtc
    24181 atgcagaaaa agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg
    24241 gcgacatggg ctttggcgct tgcaaaactt acgacctgga agtatggatc ccggcacaga
    24301 acacctaccg tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc
    24361 aggcacgttg ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt
    24421 ctggtctggc tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg
    24481 gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct
    24541 aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga
    24601 taccgaacaa taattactct tcacttacac gtaatactac tttcgagtga aaatctacct
    24661 atctctttga ttttcaaatt attcgatgta tacaagccta tatagcgaac tgctatagaa
    24721 ataattacac aatacggttt gttactggaa tcaatcgtga gcaagcttga gtgagccatt
    24781 atgaaaacga aaatccctga tgcggtattg gctgctgagg tgagtcgccg tggtttggta
    24841 aaaacgacag cgatcggcgg cttggcaatg gccagcagcg cattaacatt accttttagt
    24901 cggattgcgc acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg
    24961 agcgcctgta cagttaactg tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt
    25021 gaaatcaaat atgtcgaaac ggacaatacc ggcgatgaca attacgacgg cctgcaccag
    25081 gttcgcgcct gcctgcgtgg gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg
    25141 aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca aattcgagcg cattagctgg
    25201 gaagaagcct acgacatcat cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac
    25261 gagtctatct atctgaacta tggcaccggt acgctgggcg gcaccatgac ccgctcctgg
    25321 ccgccgggaa ataccctggt cgcgcggctg atgaactgct gcggcggcta tctgaaccat
    25381 tacggcgact actcctccgc gcaaattgcg gaaggtttga actataccta cggcggctgg
    25441 gcagatggca acagcccgtc ggatatcgaa aacagtaagc tggtagtgct gtttggtaat
    25501 aaccctggcg aaacgcgaat gagtggcggt ggggtgactt actatcttga acaggcacgc
    25561 cagaaatcta atgcccgcat gatcatcatc gatccgcgct ataccgacac cggtgccggg
    25621 cgcgaagatg agtggatccc tattcgtccg ggaacagatg ccgcactggt taacggtctg
    25681 gcgtacgtca tgatcactga aaacctggtg gatcaggcat tcctcgataa atattgcgtt
    25741 ggctacgatg agaaaaccct gccagccagt gcgccgaaaa atggccacta taaagcttat
    25801 attctgggtg aagggccaga tggcgtggct aaaacaccgg aatgggcctc acaaatcacc
    25861 ggtgttccgg cagacaaaat catcaaactg gctcgtgaaa tcggcagtac caaaccggcg
    25921 tttatcagcc agggatgggg cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct
    25981 atctcgatgc tggcgattct gaccggtaac gttggtatta atggagggaa cagcggcgcg
    26041 cgtgaaggtt catacagctt accgtttgtc cgtatgccga ccttggaaaa cccgatccag
    26101 accagcattt cgatgtttat gtggaccgat gccattgaac gtggcccgga aatgacggcg
    26161 ctgcgtgatg gtgtacgcgg gaaagataag ctggatgtgc cgatcaaaat gatctggaac
    26221 tatgccggta actgcctggt taaccagcat tctgaaatca accgtaccca tgaaatcctt
    26281 caggatgata agaagtgcga gctgattgtg gttatcgact gccacatgac ctcatcggcg
    26341 aaatatgctg acatcctgct gcctgactgc accgcttccg aacagatgga ctttgcgctg
    26401 gatgcatcct gcgggaatat gtcttacgtg attttcaacg atcaggtgat taaaccgcgc
    26461 tttgaatgta agaccatcta tgaaatgacc agcgaactgg caaaacgtct tggcgttgag
    26521 caacagttta ctgaaggccg tacccaggaa gagtggatgc ggcatctgta tgcccagtcg
    26581 cgggaagcga ttcctgaact gccaacgttt gaagagttcc gcaagcaggg gatctttaaa
    26641 aagcgcgacc cacaagggca tcacgttgct tataaagcct tccgtgaaga tccgcaggca
    26701 aacccactga ctacgccatc gggcaaaatt gagatttatt cgcaggcgct ggctgacatt
    26761 gccgctacct gggaattgcc agaaggcgat gtgatcgatc cactgccgat ctacacgccg
    26821 ggctttgaaa gttatcagga tccgctgaac aaacagtatc cgctgcagct tacgggtttc
    26881 cactataaat ctcgcgttca ctcaacttac ggcaacgttg atgtgctgaa agcagcttgc
    26941 cgtcaggaaa tgtggatcaa cccgcttgat gcgcaaaaac gcggtatcaa caacggcgat
    27001 aaagtcagga tctttaacga tcgtggtgaa gttcatattg aggcgaaagt gacgccacga
    27061 atgatgccag gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga tgcaaaacgt
    27121 gtcgataagg gtggttgtat taacgtactg accactcaac gtccgtctcc tctcgctaag
    27181 gggaatccgt cacatacaaa ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac
    27241 aacccagtat ggatttttta ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct
    27301 ggcttgtaaa gactacaaag atttgacgcc agaagtcagc ttccgccgca tttatgaata
    27361 tgctggcggc gactggcagg aagataacgg tgtctggcac cagaacgtgt ttgcctacta
    27421 tctgtcgatt tcgtgcaacc actgcgaaga tccggcttgt actaaagtct gcccgagcgg
    27481 tgcgatgcat aaacgtgaag atggttttgt tgtggtcgat gaagatgtgt gcattggctg
    27541 ccgctactgc catatggctt gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca
    27601 tatgaccaaa tgcgatggtt gttatgaccg tgttgcggag ggtaaaaagc cgatctgtgt
    27661 tgaatcttgt ccgctgcggg cgctggattt cggccctatc gacgagctgc gtaaaaaaca
    27721 tggcgatctg gcggccgttg cgccgttgcc gcgagctcac tttaccaaac cgaatattgt
    27781 gatcaaaccc aatgccaata gccgcccgac cggggatacc actggctatc tggcaaaccc
    27841 gaaggaggtg tgagatggga agtggatggc atgaatggcc gctgatgatc ttcacggtct
    27901 tcgggcaatg tgtggcgggt ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc
    27961 tgcgagcaga agcccagcag cgtgttatcg cctgcatgtt tggcttatgg gtgctgatgg
    28021 gcattggctt tatcgcttct atgctccatc ttggttcacc gatgcgcgct tttaactcgc
    28081 tcaaccgggt aggggcttca gcactcagta acgaaatcgc cagcggttcg atcttttttg
    28141 ccgtaggcgg catcggctgg ttgctggcaa tgctgaaaaa gctgtcaccg gcattgcgta
    28201 cgctttggct ggtggtgacg atggttcttg gcgtcgtctt tgtctggatg atggtgcgtg
    28261 tgtataacag cattgatacc gttccgacct ggtacagcat ctggacgccg atgggcttct
    28321 tcctgacgat gttcatgggc ggcccgttac tggggtacct gttgctgagt ctggctggcg
    28381 tcgatagctg ggcgatgcgt ctgctgccag ccatttccgt actggcactg gtggtgagtg
    28441 gcgtggtgtc agtgatgcag ggcgcagagc tggcgactat tcatagttct gtgcagcagg
    28501 ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg gcggatcgtg cttttggccg
    28561 ttgccctgtg cttgtggatt gcaccacagc taaaaggtta tcagcctgcg gttccgctac
    28621 tatccgtctc attcattctg cttctggcag gggaattaat cggtcgcggc gtattctatg
    28681 gtttgcatat gaccgtgggg atggccgtcg caagctaatc agtaacaacc ggggtttcgg
    28741 ccccggttct cttttatttc tgcttcgtta acgtgtcata actggtcatc aaattacgat
    28801 aatcaggaat atggttagag aacaacgtcg ccagtccttc aatatcatta cgccagtcac
    28861 ggtgcagctc gcaagccacg ccgaaccatg tcatcaattg cgcacctgct tgcgacatgc
    28921 gatcccatgc cgaatgccgg gtaatttcat taaaggtccc tgacgcgtca gtcacaacaa
    28981 acacatcaaa cccttcttca atggctgaaa gcgccgggaa tgccacgcaa acttcggtta
    29041 ccacaccggc aataattaac tgttttttac ctgtcgcttt gactgctttt acaaaatctt
    29101 cgttatccca ggcgttaata ttgcccgggc gagcaatgta aggtgcatcg gggaattgtg
    29161 ctttcagttc tggaactaat gggccgttag gaccggtttc aaaactggtg gtaagaatag
    29221 ttggtaagtt gaaatactta gccaggtcac ccagcgccag cacgttattt ttaaacttat
    29281 cgggttcgat atcccgtaca agggaaagta aaccagcctg gtgatcaaca agcaaaacag
    29341 cagcatcatt tttatcaaga cgaacatacg gtttggtcat cctcttctcc tttcgagagt
    29401 cagtggtacg cctgaaatcg gtcagacgtg atgcattgct ctgaaagcat agacgggaaa
    29461 tatgagtttg ctgtgaccat gaaatttttc gactgaacga agcgtatgaa atttgtgtta
    29521 gatcaataaa aacaatcaga tgagattaat catctcttca gtattagcgt tatgaatgag
    29581 taatataaat cgataatacc gccgttgcga tatgcgcgta ttttcaccca ttgacaatgt
    29641 ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct cacggtttta attcatgtcc
    29701 acgtataccc ggcctgtcat gcttttgctg tctggcctgc ttttgttgac tctggcgatc
    29761 gcggtgttaa atacactcgt gccgctttgg ctcgcccagg aacacatgtc cacatggcag
    29821 gtaggcgttg tcagctcatc ctattttacc ggcaaccttg tcggtacatt gctgacaggg
    29881 tatgtcatta aacgcattgg ttttaaccgc agctattatc tggcctcctt catttttgcc
    29941 gctggctgtg ccggccttgg cctgatgatt ggattctgga gttggttggc gtggcgtttt
    30001 gtcgcgggcg tcggctgtgc catgatttgg gtagtcgttg agagcgcgct gatgtgcagt
    30061 gggacgtcac gtaaccgtgg gcgtttgctt gctgcataca tgatggttta ttacgtggga
    30121 acgtttttag gccagttact ggtcagcaag gtctcaaccg agctgatgag cgtattgccg
    30181 tgggttacag gtttgacatt ggcagggatc ttaccgctgt tgtttacgcg tgtcctgaat
    30241 cagcaggctg aaaaccatga ttcgacgtca attacggcaa tgctaaaact ccgtcaggcg
    30301 cggcttggcg tgaatggctg cattatctca ggaatcgttc tgggatctct atatggcctg
    30361 atgcctctgt acctcaatca caaaggggtg agcaatgcca gtattggttt ctggatggcg
    30421 gtactggtca gtgcgggtat cctcggacaa tggccgattg gacgtctggc ggataagttt
    30481 ggtcgactgc tggttttgcg tgttcaggtc tttgtcgtca ttctcggcag tattgcgatg
    30541 cttagccagg cggcgatggc cccagcgtta ttcatcctcg gtgccgctgg ctttacgcta
    30601 tatccggtgg cgatggcatg ggcttgcgag aaagttgaac atcatcaact ggtggcgatg
    30661 aaccaagcct tactgttgag ctatactgtg ggaagtctgc ttggcccgtc atttaccgct
    30721 atgctaatgc agaatttctc cgataattta ttgtttatca tgatcgccag cgtatcgttt
    30781 atctatttgc tgatgctgct gcgcaacgcc ggtcatacgc cgaaacccgt tgctcacgtg
    30841 taaatgaatt caagcagagt gtgaacttac tgtttcacac tctgcttttt tgtttcttct
    30901 atctgacttg ctttattcca aattttattc gtttaaaaat aaaatgtgta gcaggttata
    30961 attttgcatt tcgctatttc cgcacttctt atttgccgcg cataatccct cgttttacag
    31021 atgcccctat aattttggcg aaggatttgt ctatggctgg gaatgttcag gaaaaacagc
    31081 tgcgatggta caacattgcg ctgatgtctt ttatcactgt ctggggtttt ggcaacgttg
    31141 ttaataacta cgccaaccag gggctggtgg ttgttttttc atgggtgttt atctttgcgc
    31201 tctatttcac accttatgcg ctaattgttg gtcagttagg ctcgaccttc aaagatggga
    31261 agggcggggt cagtacctgg attaaacaca cgatgggacc cggactggct tatctcgccg
    31321 cgtggaccta ctgggtggtg catattccct atctggcaca aaaaccctag gcaattctga
    31381 ttgcgctcgg ttgggcgatg aaaggcgacg gttcgctaat caaagaatat tcagtcgtag
    31441 cgttacaggg gttaacgctg gtgctgttta tcttctttat gtgggttgct tcacgcggta
    31501 tgaaatcgct gaaaatcgtc ggttctgtgg cagggattgc tatgtttgtt atgtcgctcc
    31561 tgtatgtggc gatggcggta accgcgcctg caattactga agtgcatatt gcgaccacaa
    31621 acattacctg ggaaacgttc attcctcata tcgactttac ctacattacc actatttcaa
    31681 tgctggtttt cgcggttggc ggagcagaga agatttctcc ttacgttaat caaacgcgca
    31741 acccaggaaa agaatttcca aaagggatgt tatgcctggc ggtgatggtt gcggtttgtg
    31801 ccattctggg ctcgctggcg atggggatga tgtttgattc gcgtaatatc ccggatgact
    31861 taatgaccaa cggtcagtat tacgcctttc agaagctggg cgagtattac aacatgggta
    31921 atactttaat ggtgatttac gccattgcga ataccctggg acaagtagcg gcgctggtat
    31981 tctcgattga tgccccgctt aaagtgctat taggtgatgc tgacagcaaa tatattccag
    32041 ccagtttatg tcgtaccaac gcttctggta cgcccgttaa tggctatttt ctgaccctgg
    32101 tactggtggc gattctgatt atgctgccga ctctcggcat tggtgatatg aacaatctct
    32161 ataaatggct gttgaacctt aattcggtag tgatgccgct gcgttattta tgggtatttg
    32221 ttgcatttat tgcagtcgtt cgcctggcgc agaaatataa accagaatat gtctttattc
    32281 gtaataagcc tctggcaatg accgtcggga tttggtgttt tgcctttacc gcctttgcct
    32341 gtttgacggg gatcttcccg aaaatggaag ccttcactgc agagtggacc ttccagttgg
    32401 cgctgaatgt tgcaacgccg tttgtgctgg taggattagg actgatattc ccgctgctgg
    32461 cgcgtaaagc gaatagtaaa taattattgt ggtcggtgac gctgactctg gcagatctga
    32521 cccgttttta cataatcgag aaatgcgcgc aagccgcagg acatatactg gcggtttggg
    32581 taatagatct ggaagcctgg acgctctgtg atccagtctt ccagtacaca ttctaaacga
    32641 ccggtatcaa ggtactcctt gatctgttca tagagcagat acccgatacc agcacccatc
    32701 aaaacggcat ccagttcagc atcaacatc
//
LOCUS       DAZSBF010000023        32556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZSBF010000023 DAZSBF010000000
VERSION     DAZSBF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.023"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            189..1562
                     /gene="aroP"
                     /locus_tag="ACHXWD_000805"
     CDS             189..1562
                     /gene="aroP"
                     /locus_tag="ACHXWD_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR7619802.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1605..2459)
                     /gene="ampE"
                     /locus_tag="ACHXWD_000806"
     CDS             complement(1605..2459)
                     /gene="ampE"
                     /locus_tag="ACHXWD_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR7619803.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2456..3007)
                     /gene="ampD"
                     /locus_tag="ACHXWD_000807"
     CDS             complement(2456..3007)
                     /gene="ampD"
                     /locus_tag="ACHXWD_000807"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR7619804.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3095..3988
                     /gene="nadC"
                     /locus_tag="ACHXWD_000808"
     CDS             3095..3988
                     /gene="nadC"
                     /locus_tag="ACHXWD_000808"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR7619805.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4191..4631
                     /gene="ppdD"
                     /locus_tag="ACHXWD_000809"
     CDS             4191..4631
                     /gene="ppdD"
                     /locus_tag="ACHXWD_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR7619806.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4641..6027
                     /gene="gspE"
                     /locus_tag="ACHXWD_000810"
                     /pseudo
     CDS             4641..6027
                     /gene="gspE"
                     /locus_tag="ACHXWD_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            6017..7219
                     /gene="hofC"
                     /locus_tag="ACHXWD_000811"
                     /pseudo
     CDS             6017..7219
                     /gene="hofC"
                     /locus_tag="ACHXWD_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(7254..8297)
                     /gene="guaC"
                     /locus_tag="ACHXWD_000812"
     CDS             complement(7254..8297)
                     /gene="guaC"
                     /locus_tag="ACHXWD_000812"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR7619807.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8453..8497)
                     /locus_tag="ACHXWD_000813"
     CDS             complement(8453..8497)
                     /locus_tag="ACHXWD_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619808.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8522..9142
                     /gene="coaE"
                     /locus_tag="ACHXWD_000814"
     CDS             8522..9142
                     /gene="coaE"
                     /locus_tag="ACHXWD_000814"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR7619809.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9142..9885
                     /gene="zapD"
                     /locus_tag="ACHXWD_000815"
     CDS             9142..9885
                     /gene="zapD"
                     /locus_tag="ACHXWD_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR7619810.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9895..10092
                     /gene="yacG"
                     /locus_tag="ACHXWD_000816"
     CDS             9895..10092
                     /gene="yacG"
                     /locus_tag="ACHXWD_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR7619811.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10192..10581)
                     /gene="mutT"
                     /locus_tag="ACHXWD_000817"
     CDS             complement(10192..10581)
                     /gene="mutT"
                     /locus_tag="ACHXWD_000817"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR7619812.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10641..13346)
                     /gene="secA"
                     /locus_tag="ACHXWD_000818"
     CDS             complement(10641..13346)
                     /gene="secA"
                     /locus_tag="ACHXWD_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR7619813.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13408..13920)
                     /gene="secM"
                     /locus_tag="ACHXWD_000819"
     CDS             complement(13408..13920)
                     /gene="secM"
                     /locus_tag="ACHXWD_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR7619814.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14151..15068)
                     /gene="lpxC"
                     /locus_tag="ACHXWD_000820"
     CDS             complement(14151..15068)
                     /gene="lpxC"
                     /locus_tag="ACHXWD_000820"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR7619815.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15169..16320)
                     /gene="ftsZ"
                     /locus_tag="ACHXWD_000821"
     CDS             complement(15169..16320)
                     /gene="ftsZ"
                     /locus_tag="ACHXWD_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR7619816.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16381..17643)
                     /gene="ftsA"
                     /locus_tag="ACHXWD_000822"
     CDS             complement(16381..17643)
                     /gene="ftsA"
                     /locus_tag="ACHXWD_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR7619817.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17640..18470)
                     /gene="ftsQ"
                     /locus_tag="ACHXWD_000823"
     CDS             complement(17640..18470)
                     /gene="ftsQ"
                     /locus_tag="ACHXWD_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR7619818.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18472..19392)
                     /gene="ddlB"
                     /locus_tag="ACHXWD_000824"
     CDS             complement(18472..19392)
                     /gene="ddlB"
                     /locus_tag="ACHXWD_000824"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR7619819.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19385..20860)
                     /gene="murC"
                     /locus_tag="ACHXWD_000825"
     CDS             complement(19385..20860)
                     /gene="murC"
                     /locus_tag="ACHXWD_000825"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR7619820.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20914..21981)
                     /gene="murG"
                     /locus_tag="ACHXWD_000826"
     CDS             complement(20914..21981)
                     /gene="murG"
                     /locus_tag="ACHXWD_000826"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR7619821.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(21978..23222)
                     /gene="ftsW"
                     /locus_tag="ACHXWD_000827"
     CDS             complement(21978..23222)
                     /gene="ftsW"
                     /locus_tag="ACHXWD_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR7619822.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23222..24538)
                     /gene="murD"
                     /locus_tag="ACHXWD_000828"
     CDS             complement(23222..24538)
                     /gene="murD"
                     /locus_tag="ACHXWD_000828"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR7619823.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24541..25623)
                     /gene="mraY"
                     /locus_tag="ACHXWD_000829"
     CDS             complement(24541..25623)
                     /gene="mraY"
                     /locus_tag="ACHXWD_000829"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR7619824.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25617..26975)
                     /gene="murF"
                     /locus_tag="ACHXWD_000830"
     CDS             complement(25617..26975)
                     /gene="murF"
                     /locus_tag="ACHXWD_000830"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR7619825.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26972..28465)
                     /gene="murE"
                     /locus_tag="ACHXWD_000831"
     CDS             complement(26972..28465)
                     /gene="murE"
                     /locus_tag="ACHXWD_000831"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR7619826.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28452..30218)
                     /gene="ftsI"
                     /locus_tag="ACHXWD_000832"
     CDS             complement(28452..30218)
                     /gene="ftsI"
                     /locus_tag="ACHXWD_000832"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR7619827.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30234..30599)
                     /gene="ftsL"
                     /locus_tag="ACHXWD_000833"
     CDS             complement(30234..30599)
                     /gene="ftsL"
                     /locus_tag="ACHXWD_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR7619828.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30596..31537)
                     /gene="rsmH"
                     /locus_tag="ACHXWD_000834"
     CDS             complement(30596..31537)
                     /gene="rsmH"
                     /locus_tag="ACHXWD_000834"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR7619829.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31539..31997)
                     /gene="mraZ"
                     /locus_tag="ACHXWD_000835"
     CDS             complement(31539..31997)
                     /gene="mraZ"
                     /locus_tag="ACHXWD_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR7619830.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7731 a         9151 c         8030 g         7644 t
ORIGIN      
        1 aactcatctt caaccatgca taaagcgggt gcattcgctg ccgcatacca ttattcttga
       61 tctgacggaa gtctttttgt aacaattcaa acttctttga tgtaaaaaaa ttaatacaac
      121 aaacggaatt gcaaacttac acacgcatca ctgcgtagat caaaaaaaca atcaccgcac
      181 gaggtttcat gatggaaggt caacagcacg gcgagcagct aaagcgcggc cttaaaaacc
      241 gccatattca gcttatcgcg ctgggtggcg cgatagggac agggctattc ctgggtagcg
      301 cctccgtaat acagtccgca gggccaggga ttatcctggg ttacgccatt gctggtttta
      361 tcgcctttct gatcatgcgt cagctgggtg aaatggtggt cgaagaacct gtcgcaggct
      421 cctttagcca ctttgcttat aaatactggg gcagttttgc cggtttcgcc tctggctgga
      481 actactgggt actgtacgtt ttagttgcca tggcagagct gactgccgtg ggtaaataca
      541 ttcagttctg gtatccggaa atcccaacct gggtttctgc cgccgtattc tttgtggtga
      601 ttaacgccat caacctgacc aacgttaaag tgtttggcga gatggagttc tggtttgcca
      661 ttatcaaagt tattgcgata gtagcgatga tcatcttcgg cggctggctg ctgttcagtg
      721 gcaacggcgg cccgcaggcg agcgttagca acctgtggga tcaaggtggt ttcctgccgc
      781 acggcttcac cggactggtg atgatgatgg cgattatcat gttctcgttc ggtggtctgg
      841 aactggtggg gatcaccgca gcagaagctg ataacccgga gcaaagtatc ccgaaagcga
      901 ctaaccaggt tatctaccgc atcctgattt tctatattgg ttcgttagcc gttctgctct
      961 cactgatgcc gtggacccgc gttaccgccg ataccagtcc gtttgtgctg atcttccacg
     1021 agttaggcga tacctttgtg gcgaatgcgc tgaacatcgt ggtactgact gcggcgctct
     1081 ccgtgtacaa cagctgcgta tattgcaaca gccgtatgct gtttggtctg gcacaacagg
     1141 gtaatgcgcc aaaagcgctg gcgtctgtcg ataaacgcgg cgtaccggtt aacaccattc
     1201 tggtgtctgc gctggttaca gcattgtgcg tattgattaa ctatcttgct ccggaatccg
     1261 catttggcct gttaatggca ctggtggtat ccgcactggt gatcaactgg gcgatgatca
     1321 gtctggcgca tatgaagttc cgtcgcgcca agcaggaaca aggcgtggta actcgcttcc
     1381 ctgctctgct ttatccgctg ggtaactgga tctgcctgct gtttatggcg gcggtactgg
     1441 tgattatgct gatgacccca ggaatggcga tttcggtata cctgatcccg gtatggctga
     1501 tcgtgttagg tatcggctat ctgtttaaag agaaaaccgc caaagccgta aaagcgcatt
     1561 aatctctcta cgccctcacc catgcagggt gagggcaata atctttacac caacgctccg
     1621 taaatcgtca gtagcgcaat caccaccacg accacgaacg aggttttctt cgccattgaa
     1681 accgctgcct tcggcgtctc caccttatcg acatgcggtt cacgcgccag agagaactgc
     1741 gccagacgcg ttaacacctg atactgcgaa gtatggaagt cacccagcga agcaaaccag
     1801 gccggtaacg ctttctcccc atgaccgatc aaggcatata ccacacccgc aagacgaacc
     1861 ggcacccaat ccagtacatg aagcacggca tcaatgccgg actgtaaacg atgatgcggc
     1921 gtctggtatc gcgccagcca gtattgccat gcacgcaaaa acgcataccc catcagcgta
     1981 acgggtcccc aggttccccc cacaatcaac cagaacagcg gtgcaagata aaaacgaaag
     2041 ttaatccaca gcaatgcatt ttgcagctca cgcaaatact cacgttcgtc gcagcctgcc
     2101 gggacgccgt gaatcatggt gagttcgcca gccatcgtgg cacgggcatg gctatcatta
     2161 cgtgaagcag ctgtcagata agcatgataa tgaagacgaa ctttacctgc gccaatacac
     2221 agcaaaccaa tcagcagcca caccagcagc gtgggaacgt tgaacaatac tccctgcaat
     2281 gcgcgtaaca gtaaaaaagt cacgcccatc gcaataatgg tcatgcctaa cgtgcgcccg
     2341 agagaaaaat gtttcacccg ccgaaagaac gcttcaagac gatgatcaag ctgccagtgc
     2401 tcgcccaact taaacaggcg ctcgaaaatt aacaccagta aggttgtaaa tagcgtcatg
     2461 ttgtctcctt gctgaccagc gcacgaaacc gtgcccaatc aaatgcagga ccgggatcgg
     2521 ttttccgatc cggcgcaata tcacaatggc ccgtcatgtt attagcgata tcgggatagc
     2581 gatctatcag tgcccgcgta accgccgcaa gctgttgata ctgcgcatcg gtatacgcca
     2641 gagtatcggt gccttcaagc tcaatcccaa tagaaaaatc attgcagcgt tcacgcccct
     2701 gatactgaga gactcccgca tgccaggcac gtttatcgaa aggaacatac tggactattt
     2761 caccatcacg gcgaatcaaa cagtgagcgg agacgcgcaa atgggcgatc tcagcaaaga
     2821 aaggatgtgc ctgcggatca atagttccag tgaataatgc gtcgatccac ggaccgccaa
     2881 actcgcctgg cggcaggcta atattgtgca ccaccagcag ggtgggtgtt tcgtcatccg
     2941 ggcggcaatc gtaatgtggt gagggaacgc ggcgcgcgcc aaccagccac ccctgttcta
     3001 acaacatgca ggatctcctt atatgtggtg ctaatacccg gttcagagta gcatgtttct
     3061 accttatgat tcgttagcta tctggagttt taacatgccg cctcgccgct ataaccctga
     3121 cacccgacgt gacgagctgc tggaacgcat taatctcgat atccccggcg cggtggccca
     3181 ggcgctgcgg gaagatttag gcggaacagt cgatgccaac aatgacatta cggcaaaact
     3241 tttaccggaa aactctcgct ctcatgccac ggtgagcacc cgcgagaatg gcgtattttg
     3301 cggcaaacgc tgggttgaag aggtgtttat tcaactggca ggcgacgatg tcaccataat
     3361 ctggcatgtg gatgacggcg atgtcatcaa cgccaatcaa cccttgttcg aacttgaagg
     3421 cccatcccgc gtgctgttaa cgggcgaacg cactgcgctt aattttgtgc aaaccctttc
     3481 aggagttgcc agtaaggtac gccactatgt cgaattactg gaaggcacca acacgcagtt
     3541 gttggatacg cgcaaaacct tacctggcct gcgttcagct ctgaaatacg cggtactttg
     3601 cggcggcgga gcgaatcacc gtctggggct ttctgatgcc ttcctgatca aagaaaatca
     3661 tattattgcc tccggctcag tgcgccaggc cgtcgaaaaa gcgtcctggc tgcacccgga
     3721 tgcgccagta gaagtcgaag tagagaatct ggaagaactt gatgaagccc tgaaagcagg
     3781 agccgatatc atcatgctgg ataacttcga aacagaacag atgcgcgaag ccgtcaaacg
     3841 caccaacggc aaggcgctac tggaagtgtc tggcaacgtc actgacaaaa cactgcgcga
     3901 atttgccgaa acgggcgtgg actttatctc cgtcggtgcg ctaactaaac acgtacaagc
     3961 actcgacctt tcaatgcgtt ttcgctaatt gctgacagat ttatccgctc catcgcgggc
     4021 ggataatctc cccttcccca actttcttcg taacgcctcg caaatttctt gtcttttgct
     4081 gcaacgaaat aaaaaacctc ggaagctgct ttcccgtttc gttttttgcc cctcgcttgt
     4141 gctgatggcg tttgccaaag tagcgccaac caaatcaagg agcgaaacag atggacaagc
     4201 aacgcggttt tacacttatc gaactgatgg tggttattgg catcattgcc attttaagcg
     4261 ccattggtat tcccgcttat caaaactacc tgcgcaaagc tgcactcacc gacatgctac
     4321 aaacctttgt gccttaccgt acagccgtag agttgtgcgc gctggaacat ggtggattag
     4381 atacctgcga cggtggcagc aatggcattc cctcgcctac caccacccgc tatgtttcag
     4441 ccatgagtgt ggcaaaaggc gtggtgtcgc tgaccgggca agaaagtctc aatggactaa
     4501 gcgtcgtcat gacaccgggt tgggataacg caaacggcgt caccggctgg gcgcgcaact
     4561 gcaatattca aagtgacagc gcattgcagc aagcctgcga agatgtcttc cgctttgatg
     4621 acgccaacta aggagcggca atgaatattc cacagctcac cgccctgtgt ctgcgttatc
     4681 agggagtctt gctggatgcc agcgaagagg tggttcatgt tgcggtagtc gatgcacctt
     4741 cgcatgagtt gctggacgca ttgcatttcg ctaccaccaa acgtattgag atcacctgct
     4801 ggacgcgcca acaaatggaa ggccacgcca gtcgcacaca acagacattg cccgtagctg
     4861 ttcaggagaa gcatcagccc aaagcagagt tgctgactcg aacgttacaa tctgcgctgg
     4921 aacaacgtgc gtctgatatt catatcgaac cagcggacaa tgcctaccgc atccgcttgc
     4981 gtatcgacgg cgtattgcat cctttaccgg atgtttcacc ggatgccgga gtcgcattaa
     5041 ccgccagatt aaaagtgctg ggaaacctgg atattgcgga acatcgcctg ccgcaggacg
     5101 ggcaattcac tgtcgaactg gcaggaaacg ccgtctcatt tcgtattgcg accttaccat
     5161 gtcggggtgg tgaaaaggtg gtattaaggt tgttacagca ggtgaaccag gcactggatg
     5221 ttaacacgct gggaatgcag ccgttacaac tggcggactt tgctcatgcc ttgcaacaac
     5281 cacagggact ggtgctggta actggcccta caggcagcgg caaaacggtc acgctttata
     5341 gtgccctgca aaagctgaat accgctgaca ttaatatttg tagcgtcgaa gatccggttg
     5401 agatccccat agccggacta aaccagacgc aaatccatcc gcgtgccggg ctcacctttc
     5461 agggcgtttt gcgtgcgtta ttgcgccagg atcctgacgt catcatgatc ggagagatcc
     5521 gctatggctt taacggcaga aattgccatt aaagccgcgc aaaccggtca cctggtgttg
     5581 tctaccctac acactaattc cacctgcgaa acgctggtac gtttacagca aatgggagtc
     5641 gcccgctgga tgctctcatc agcgcttacg ctggtaatag cccagcgtct ggtacgtaaa
     5701 ctttgcccac attgtcgcca gcagcaaggg gagcccatcc atattccagt caatgtatgg
     5761 ccgtcgccgc tgccccactg gcaggcaccc ggttgtgtac attgctacca cggtttttat
     5821 ggtcgtacgg ccttatttga agttctgccc ataacgccgg tcattcgtca gcttatttcc
     5881 gctaataccg acgttgaatc gctggaaacg cacgcacgac aggcgggtat gcgtacgctt
     5941 tttgaaaacg gctgcctggc cgtggagcaa ggcttaacca cctttgaaga gttaatccgc
     6001 gtactgggga tgccgcatgg cgagtaagca actctggcgc tggcatggca taaccggcga
     6061 cggcaatgcg caagatggga tgctatgagc agagagccgt gctttgctgc tcatggcact
     6121 acagcaacag atggttaccc cacttagcct gaagcgaatc gccatcaatt ctgcgcagtg
     6181 gcgaggagat aaaagcgcgg aagtcattca tcaactggcg acgctactca aagccgggtt
     6241 aacgctttct gaagggctgg cactgctggc ggaacagcat cccagtaagc aatggcaagc
     6301 gttgctgcaa tcgctggcgc acgatctcga acagggcatt gctttttcca atgccttatt
     6361 accctggtca gaggtatttc cgccactcta tcaggcgatg atccgcacgg gtgaactgac
     6421 cggtaagctg gatgaatgct gctttgaact ggcgcgtcag caaaaagccc agcgtcagtt
     6481 gaccgacaaa gtgaaatcag cgttacgtta tcccatcatc attttagcga tggcaatcat
     6541 ggtggttgtg gcaatgctgc attttgttct gccggagttt gccgctatct ataagacctt
     6601 caacacccca ctaccggcac taacgcaggg gatcatgacg ctggcagact ttagtggcga
     6661 atggagctgg ctgctggtgt tgttcggctt tctgctggcg atagccaata agttgctgat
     6721 gcgccgaccg acctggctta tagtgcggca gaaattgctg ttacgcatcc cgattatggg
     6781 ttcactgatg cggggacaaa aactcacgca gatctttacg attctggcgc tgacacaaag
     6841 tgcaggcatt acttttttgc agggcgtaga gagcgtcaga gaaacaatgc gctgcccgta
     6901 ctgggtgcaa cttctgacac aaatccagca cgatatcagt aacggtcatc ccatctggct
     6961 ggcgctaaaa aataccgggg agtttagccc gctctgtttg caattagtga gaacaggaga
     7021 ggcatccggc tcgctggacc tcatgttaga caacctcgcc catcatcatc gggataacac
     7081 aatggcgctg gcggataacc tcgcagcctt actggaaccg acgttgctga tcataacggg
     7141 aggaattatc ggtacgctgg tggtggcgat gtatctgcca attttccatt taggcgatgc
     7201 gatgagtggg atgggataac gctggcgtgt tgctccacac cagcgttgga agattacagg
     7261 ttgttgaaga tgcggttttc ttgttcctgc acacggataa atgtggtgcg cttggtcagt
     7321 tctttcaggc gtgaagcccc aacgtatgta caagctgaac gcaggccgcc caaaatatca
     7381 cgcgcggtat tttcaaccgg gcctcgcagc ggcagcttaa cggttttacc ttctgctgcg
     7441 cgatattccg caacgccgcc aacgtgacgt ttcatcgcag actcggagct catgccgtag
     7501 aacagcataa atttctcgcc gttctcctca acgatgcgac cgccgctctc ttcgtggccc
     7561 gccagcatgc cgccaagcat gacgaaatcg gcaccgccgc caaaggcttt tgcgacatcg
     7621 cccggcgtgg tgcagccacc gtcgctgacg atcattccgc ccagaccgtg cgccgcatcg
     7681 gcacattcga ttaccgcaga aagctgcgga taaccgacgc ctgttttgac gcgagttgta
     7741 caaacagaac ctgggccaat gccaacttta acgatatcag caccggagag gataagctcc
     7801 tcacacattt caccagtcac tacgttgcca gcacaaatgg ttttggtcgg ccacgcttca
     7861 cgcgctttcg caacgaactg cacgaagtgt tcggaataac cattcgccac atcaatacaa
     7921 acgaagttta atgccgggtt caggtcgaga atctgtttag ttttttcgaa atccgcatca
     7981 gacgtaccgg tagaaaccat cacatgtttc agcacatcag cggaagaatt gttgataaac
     8041 gcttgccact cttcgacaga atagtgttta tgcacagcag tcaaaatatc aaaagaagcc
     8101 agcgcagagg ccatagaaaa tgtacctacg gtgtccatat ttgcggcgat aatcggcacg
     8161 ccggaccagc tctgacctga atgtttgaag gtgaattgac gttccagttc aacatcggaa
     8221 cggcttttaa gagtggagcg tttagggcgg atgagaacgt ctttaaaacc taacttcaga
     8281 tcttcttcaa tacgcatgtg cggattcctg gggttaatgg ctataagggt aagagcgcaa
     8341 ctccagtgac gctatcatac gcactaatca atgcggcgca agactgcgaa attgtctttt
     8401 tttacgctac aatcccataa atttacctgg taaatagtga gcgaacgttt catcaatttc
     8461 gctttcgtgc actcattttt aacgttttga taatcatgat gaattcccgg gaatataaat
     8521 tatgaggtat atagttgcct taacgggagg cattggcagt ggcaagagta ccgttgccaa
     8581 tgcgtttgct gatctcggaa ttaacgtcat tgatgccgat attattgcgc gtcaggtggt
     8641 tgaaccaggt gcacctgcgc tacatgccat tgctgatcac tttggcgcta acatgattgc
     8701 tgctgatgga acattgcagc gccgggcctt gcgcgagcgg atcttcgcca acccggaaga
     8761 gaaaaactgg cttaacgccc tgctgcatcc gctgattcag caagagacgc aacaccagat
     8821 ccagcaagct acttccccct atgtactgtg ggttgtgcca ttgctggtag aaaactcact
     8881 gtataaaaaa gcgaatcgag tgctggtggt ggatgtcagc ccagaaacgc aacttaagcg
     8941 caccatgcag cgcgatgatg taactcgcga gcatgtcgaa caaatccttg ctgctcaggc
     9001 aacgcgcgaa gcccgccttg ccgtggcaga tgacgtcatt gataataacg gcgcaccgga
     9061 tgctatcgca tcggatgttg cccgcctgca cgcacactat ttgcagcttg cgtcgcagtt
     9121 tgtctcacag gaaaaaccgt aatgcagacc caggtccttt ttgaacatcc actaaatgaa
     9181 aaaatgcgta catggctgcg cattgagttt ttgattcagc aactcaccgt taatttaccc
     9241 atcgttgacc acgctggcgc gctgcatttc ttccgtaatg tcagtgaatt actggatgtt
     9301 ttcgagcgcg gcgaagtccg cactgagctg ttgaaagaac ttgaccggca gcaacgtaaa
     9361 ctccagacct ggattggcgt gcctggcgtg gaccagagcc gtattgaagc attaattcag
     9421 cagttaaaag cggcggggag cgtattaatt tccgcgccgc gtatcgggca atttctgcgt
     9481 gaagatcgtt tgattgctct ggtgcgtcag cgactgagca tcccaggcgg ctgttgcagc
     9541 tttgatttac ctacattgca catttggctg catctacccc aggcgcagcg cgacagccag
     9601 gtagaaacct ggattgccag cctgaacccg ctcacccagg cacttaccat ggtgctggat
     9661 ttaattcgcc agtcggcccc cttccgtaaa caaaccagtc tgaatggttt ttatcaggat
     9721 aacggtggcg atgccgactt gctgcgcctg aatctgtcgc tcgattcaca gctttatccg
     9781 caaatttccg gacataagag ccgttttgcc attcgtttta tgccgctgga cactgaaaac
     9841 ggacaggtac cggaacgtct ggatttcgaa ctggcctgtt gctaaggagt aaaaatgtca
     9901 gaaactatta cggtgaattg cccaacctgc gggaaaacgg tggtgtgggg tgaaatcagc
     9961 ccgtttcggc cattttgctc caaacgttgt cagctgatcg acctcggaga atgggctgct
    10021 gaagaaaaac gaatccccag cagtggcgat ctttccgaaa gcgatgactg gagcgaagag
    10081 ccaaagcagt gacatttgtc tgatgccgca cgtaggcctg ataagacgcg gacagcgtcg
    10141 catcaggcat cttgtgcaaa tgtcggatgc ggcgtgaacg ccttatccga cctacagacg
    10201 cttaagcttc gcaattaccg gttcattggc tggcggaaaa tcatcggcat taagaccgac
    10261 tagcgacatc cactcaccgg gttgcccttc tttaccccac ggctcccctt cccagctttc
    10321 gaccagccag aaccacagag ttatatgcct gtccgggaat tcatattcca gtttttcaaa
    10381 tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga agttcacgca ctaccgcctg
    10441 ttccggcgtt tcgcccattt caattttacc gccgggaaac tccagtttat tcgccatgtg
    10501 cgcatctgct gcgcgacgcg ttataaagat ttcattgttc tcgttgcgaa taatacctac
    10561 cgcaatttgc agctttttca ttgtcttaaa cctataaaaa aggcgcagaa tcctgcgcct
    10621 tttacttcaa ctgttagctt ttattgcagg cgaccatggc actgcttgta ttttttacca
    10681 gaaccgcacg ggcaaggatc gttacgtcct actttgcgct caccggtttg cgccgccagt
    10741 gcagctgcgg ctgcagagtc gtcatcctga tggctaagct gctgcatttg cgctaaacgc
    10801 tcggcttcca tacgacgctg ttgttccagc tcctcaacct cttcaggcat acgtacctgg
    10861 actttgctca gcgtgctgat aacttcatat tttaacgact ccagcatcgc tgcaaacatg
    10921 gagaacgatt cacgtttgta ttcctgcttc ggatctttct gtgcatagcc acgcaggtgg
    10981 ataccctgac gcagatagtc catcgccgct aggtgctctt tccacagaga gtcgagagtt
    11041 tgcagcatga cgcctttttc gaagtgacgc atcatctcag caccaaccac ttcttcttta
    11101 cgctgataca cttcgatgga ctgcgccaga attcgctcac gcagcgtctc ttcatgcagt
    11161 tctggttctt tatccagcca ctcggcaatt ggcaaatcga ggtcgaaatc gttcttcaga
    11221 cgttcctgca gccccggaat atcccacatt tcttccagtg actgtggcgg aatgtaggca
    11281 tcaatggtcg ctttgaacac atcttcacga atgctgttga tggtttcgct cacatcgctg
    11341 acatccagca gttcgttacg ctgggagtaa atggcgcgac gctgatcgtt agccacgtca
    11401 tcatattcca gcagttgctt acgaatgtcg aagttacggc tttcaacttt acgctgggcg
    11461 ttggcaatcg ctttagtcac ccacgggtgt tcaatggctt cgcctggctt catacccagt
    11521 ttacgcatca tgccggatac tcggtcggaa gcaaaaatac gcatcagcgc atcttccatc
    11581 gacaggtaga aacgggaaga accagcatcc ccctgacgac cagaacgacc gcgcaactgg
    11641 ttatcgatac gacgggattc gtgacgctcg gtaccgatga tatgcaggcc acctgcttcc
    11701 agtaccgcat cgtgacgtac ctgccagtcg gctttaattt tttcaatttg ctctgcggtc
    11761 ggattttcca gcgcggcaac ttctgcctgc cagctaccac cgagcacaat atctgtacca
    11821 cgacccgcca tattggtcgc gatagtcacc gcagccggat aacctgcctg agcaacaatc
    11881 gccgcttcgt tggcgtggaa tttggcgttc aggacgttgt gcttaatacc ggctttggtc
    11941 agttcgtttg acaccagctc cgatttttcg atggagatag tacccaccag caccggctgg
    12001 cctttcgcag tacgttcttt gatatcttca atgatcgcct gaattttttc cgcttcagtc
    12061 atgtagacca ggtccggcag atctttacga atcattggac ggttggtcgg aacaacgacg
    12121 gtatccagct tgtagatgga gctaaattcg aaagcttcgg tatcagcagt accggtcatc
    12181 cccgccagtt tttcatacag acggaagtag ttctggaagg tgatcgaagc cagcgtttgg
    12241 ttttcgttct ggatctgcac accttctttc gcttccacag cctggtgcag accatcggac
    12301 cagcgacggc cctgcatggt acgaccggtg tgttcgtcaa cgatgataac ttcaccatct
    12361 ttaacgatgt agtcgacgtc gcgggtaaac agcgcatgag cgcgcagcgc cgccgttacg
    12421 tggtgcatca gcatgatgtt ggccggagag tacagagact ccccttcatc catgatgccc
    12481 tctttcacca gcagttcttc aatcagcacc agaccacgtt cggtcaggtt cacctggcga
    12541 gatttttcgt ccaccgagaa gtggccttcg ccctggaagg tttcggagtc ttctttttcc
    12601 tgacggatca ggtgcggaat aattttattc acgcgtttat acatttccga gctgtcttct
    12661 gccgggccgg aaatgatcag cggtgtacgc gcttcatcga tcaggatgga gtccacttcg
    12721 tccaccagcg catagtgcag tttacgctgt acacgttctt cagggctgaa cgccatattg
    12781 tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt aagtgatgtc agctgcgtaa
    12841 gcttcgcgct ttgccggtgc tggcatgccc ggcaggttga taccgacagt caggccaagg
    12901 aattcaaaca gcggacggtt gttttcggcg tcacgttgcg ccaggtagtc gttgacggta
    12961 actacgtgca cgcctttacc ggttagtgcg ttcaggtaag caggcagcgt tgcggtcagg
    13021 gttttacctt caccggtacg catttcggcg atgcagcgtt cgttaagaac cataccgccg
    13081 agtaactgaa cgtcgaagtg acgcatacca aagacgcgct tacttgcctc acgtaccacg
    13141 gcgaaagctt ccgggatcag attttccagc acttcgcctt tttccagacg tgcacgaaac
    13201 tctgcggttt tccctttcag ttcttcgtcg gagagttttt ccatctccgg ttccatggca
    13261 ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc gatcgttacg actaccgaaa
    13321 actttagtta acaatttgat tagcataata aaatctcaaa cgccccgcgt tgcggagtta
    13381 ataaaatgaa gtaaaggttt attgttgtta ggtgaggcgt tgagggccag cacggatgcc
    13441 ttgcgcctgg cttatccaga cgggcgtgct gaattttgct tgtggggtaa aatgcgcata
    13501 atcaatgcga taaccctttt cagacggcgt gccttcctgg gtcagcagcg cgctgagcgt
    13561 atccagtaat gcaagatgtt gcgcctgaag aggcaaagac tcttcagcaa cgggcagtgt
    13621 ttgcggtgcc attgcgaaag aaagatgacg gattaccgtg cgaatggcat gttgatgcca
    13681 gtaatcaacg gaatagttcg aattcgggcg gcgtgtgttc gcttccagca aggccaattg
    13741 accaaagtta actttggctg aaggctcgtg gttgcgggtt gtcgcttttg cgggcgcgtt
    13801 tggttcggcg gcgttgctga gcgcaggcaa acctaaactc gccgcaacca tccctaataa
    13861 gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag cgcgtcagta ttccactcac
    13921 gttattgcca tcccgatcgt attcaaacgt atttagcgca caaatcttat cattaaagcc
    13981 tgcagtccac agcacgaatg aagataagtt gtgcggaaaa gtgcttaaga aaacagcgtt
    14041 cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa tgactggttc acctggccgg
    14101 agagagtgcc agatttgcca gtcgaatttt atacgacagt ataaatgtcg ttatgccagt
    14161 acagctgaag gcgctttgaa ggccaacggc agttctgcgt cgtcctggaa ggtcacatat
    14221 tcccaggctt cctgtttcgc caggacagcc tgcagcagtt tgttattcag tgcatgaccg
    14281 gatttataag cggtaaatgc accaataata ttgtgaccac acatgaacaa gtcaccgatc
    14341 gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac gcaggccgtc ttcgttcagt
    14401 acgcgataat cgtcaacaac gatggcacaa tcgaagctgc cgcccaggca caaaccacgg
    14461 gactgcagat attcgatatc acgcatgaaa ccgaacgtac gcgcacggct gatctggcgc
    14521 ataaacgcat cagcggagaa gttcatcgca tagcgctggt tgctggaatc aatagccgga
    14581 tggttaaaat cgatggtgaa atccagcgaa aaaccattgt acggcttaaa ttcagcccac
    14641 ttatcgccat cttcgacacg aacagtctct ttgatgcgaa caaatttttt ggcgcagttc
    14701 aactcgtcga taccggcgtc aagcagcagg tatacaaacg gagcggcgct gccgtccatg
    14761 atcgggattt ccggcgcgtt aacttcgata acaatgttat cgatgcccag gcccgcgaga
    14821 gcagcattga ggtgctctac ggttgaaatc cgtacatcat gctcgttgac cagacacgta
    14881 cagagcatgg tatcacgcac agatttggca tcggccggga aatctaccgg tggattcaag
    14941 tcggtgcgac gatagatgac cccggtgttg gccggcgcag ggcgtaacgt cagagtgact
    15001 ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga tacgtttaag tgtcctttgt
    15061 ttgatcatcg tattatctcg ccaaattacc tatccaaccg aagtgtacta tacattcggc
    15121 gggccagttt agcacaaaga gcctcgaaac ccaaattcca gtcaattctt aatcagcttg
    15181 cttacgcagg aatgctggga tatccagata atccggctct ttcgcagttt gcggcgcatt
    15241 gtcattcacg actttagcaa ccggcttctg ctcctgggtc agcggagcca tcccatgctg
    15301 ctggtagcga tccatcactg gctgctgaac ctgcttattg gtcaccagag tgatttcagg
    15361 acgtttgtcc atgccgatac ctgtcgcaac aacggttacg cgcagctcgt cattcatatc
    15421 cgggtcaaga gaagtaccga taaccacagt cgcgttgtcg gaagcaaatg cacggatggt
    15481 gttacctacc gtttcgaact catccagacg caggtcgaag cccgccgtga tgttaaccag
    15541 cacgccgcgc gcgccagaca ggtcgatatc ttccagcagc ggagaagaga tagccatttc
    15601 agcagcttct tccgcacggt cttcaccgct cgccacgcca gaacccatca ttgcgtagcc
    15661 catctcagac attacggtgc gtacgtctgc aaagtccacg ttcatcaaac ccggacgagt
    15721 aatcagttca gcgatacctt gcacagcgcc tttcagtaca tcgttcgctg cgccaaacgc
    15781 atccagcagg gagataccgc ggcccagaac tttcagcagt ttgtcgttcg ggatagtgat
    15841 cagagagtcc acatgcttgg acagttcagt gatcccctgc tccgcgaatg ccatacgctt
    15901 cttgccttca aagttgaaag gcttagtgac gacagcaacg gtcaggatac ccaaatcttt
    15961 tgccacttca gcgacgactg gtgctgcacc tgtaccggta ccaccaccca tacccgcagc
    16021 aataaagacc atgtctgcac cttccagcgc cgcacgcaat gcatcgcgat cctcatcagc
    16081 cgcattgcga ccaacttctg gattagcgcc agcgcccagt cctttggtga taccgctacc
    16141 gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct tgtgcatcgg tatttaccgc
    16201 gaagaattca acaccttcaa tacgctcgcg caccatgtgt tcaacagcat taccgccgcc
    16261 gccgccgacg ccgatgactt taatcaccgc gtcattggta agttccattg gttcaaacat
    16321 agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat catcgtcggc ctcataaaaa
    16381 ttaaaactct tttcgcagcc aactattgag tcgcttgatc cacgagccaa ctgatgctgt
    16441 aacacgtttt tctacttcag cttcaccgtt aagatgtgac tctttcccat agtgaagcaa
    16501 tcccaccgcc gtcgaataat acggctcctg agcataatcc gttaaaccgg taatgttcag
    16561 cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga gcacaggctg caagaccttc
    16621 gatctgcgct gcgccaccgg ttaatacaat gcctgccgcc aggtgatgtt taaccccttg
    16681 ttggcgaagc ttttcctgca actgcaatat ctcttcgttg accaggttga gcagctcggt
    16741 atagcgcggc tcgatcacct ctgccagggt ctgacgttgc agactccgtg gcggacgacc
    16801 gcctacgctc ggcacttcca cgctctcatc ttttccaacg atggaaccca gcgcacaacc
    16861 gtggcgaact ttaatcgctt cggcgtcgct tggcggcgtg ccaaaggcgt aagcgatatc
    16921 actggtcacg acattgccag cataaggaat taccttagtg tggcgcaatg ccccaccggt
    16981 ataaacggcg atatccattg taccaccacc gatatcgacg acgcagacac ccagttcacg
    17041 ttcatcttcc gtcaataccg aataacttga tgccagtccg gcaaatatca gttggtcaac
    17101 tttcagccca caacgttcaa ccgctttgac gatgtttttc gccatatcgt tgtgacatgt
    17161 gatcaggtgc acttttgcct gcatccgcac gcccgaaagt cctaccggat tcttgatccc
    17221 ttcctgatag tcaatcgcat actcttgcgg gatcacatgc agcacacgat gttcatcgcg
    17281 cacacgcacc gatttcgcgg tatggacgac gttttccaca tcttcttgcg tcacttcttc
    17341 ttcagaaata ggcaccatac caatttcatt ctggcagctg atgtgcttac cagaaagcgc
    17401 cagatatacc gaagagatct gacaatctgc catcaattct gcctggtcaa tggcgcgttg
    17461 tacgcacttg accacggatt cgaggtcgtt caccccgcct ttatccatac cacgtgacgg
    17521 gcagctgccc acgccaatga tattgaccat accgtcgggc agaacttccc ctactaaagc
    17581 ggcaaccttc gcggtaccaa tctccagtcc tactaccagt tttctgtccg tcgccttgat
    17641 cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag attcctctgg cggcaagggc
    17701 gcccagccta ctgccgctcc agagtcataa cgcaaatcaa cgtagctaat ccgtttgcca
    17761 tcggtttgcg cctgctgctg taaaaccgga taaagttcta caaagcgagc caaacgtttc
    17821 atcgtatcgc cccggccaag attgagctta atatcgttat tcagcgtcaa ctgccaggaa
    17881 cgccgcgcgg tcatcgccgc ttccttcaga gtaaatctgt cctttgccag catctgcccc
    17941 atttcgcgat agccctgcaa cacttcattg gcgctgcctt ccgggccata cagcattgga
    18001 agcacctgct tgctggtgcg atctggcggc acgctgaagg tgtttccttc cgcgtctacc
    18061 atatgttgat cattccaccg cgcaatcggc acatattcaa ccagatgaat cttcaattca
    18121 tcaggccact gctttctgac gctcacctgc ttaatccacg gcaggcgttg ttctatttgc
    18181 gtctggatga tgttgacatc ctgggtcata aaggtacccg gctcacccaa tgccaggatc
    18241 gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac cggtcaacac cagctttgag
    18301 agcggcaggc gttgcgcatc ttccatccag cccaacacga cccagccgct caccaacact
    18361 gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac gcgttccatt attgcggcga
    18421 gaagaaacct cttcttcgct atttcgcgtg ttcagagcag cctgcgacat attagtccgc
    18481 cagttccaga attcgtacta ccaactgcga gaagctcata cctgcctgac gtgccgccat
    18541 cggcaccagg ctgtggctgg tcatacccgg tgaggtattg gcttccagca gataaaactg
    18601 tccatcgctg tccagcataa cgtcaatacg tccccatcct ttgcaaccta acgtcgtcca
    18661 tgctttcagc actaatgcct gcaaattggc ctcttgtgac gcttccagac ctgcggggca
    18721 gaaatactgt gtctcatcag agagatactt cgcctcataa tcatagaagg ttccggacgg
    18781 ttgaatacgt attgacggta aaatttcttc accgagtatc gcaaccgtga actccggccc
    18841 acttagccat ttttcaatca atacttcttc atcgtgctga aatgccaatc ttaatgcatc
    18901 ttgtagagca ttttctgcta ctacttttga cattcccaca ctggaacctt cgcggctcgg
    18961 cttaacgata accggcaaac ccagagcaga aatttctgct aactgcttat cgctcaggcc
    19021 tttttcaaac tctgcgcggg ttaacgctac ccacggcgcg accggtaaac cggcaccttg
    19081 ccacagcagt ttgctgcgta gtttatccat tgaaagcgca gatgccatca ctccgctgcc
    19141 ggtataaggc aagcccatca gctcaagcat gccctgcagc gtaccatctt caccgccgcg
    19201 accgtgtagc gcgataaaca ctttctgaaa acccatcgac ttcagttgcg tcacgtcgac
    19261 ttctttcggg tcgacaggat acgcatcaat accgccttca cgcagcccgg ctaacaccgc
    19321 tgcgccagaa ttcagagaaa cttcccgctc agcggaagtc ccgcccaaca ggaccgcaat
    19381 tttatcagtc atgttgttct tcctccggag tttgcggctt cagtttgatt tcagctaaag
    19441 aacgggcaat ttttccgata ttaccagccc cctgaacgag aatcaggtcg ttaccgatta
    19501 ataccggtgc cagcatctcg gctacctgcg cagggtccgg caccagaatg ggatcaattt
    19561 tcccacgtcc acgaattgtg cgacacagcg aacggctgtc cgctcccgga attggcgctt
    19621 cgccagccgg atacacttcc agcatcaaca gggtatcaac ctgcgtcagc acattggcga
    19681 aatcatcata caggtcgcgc gtacgggtaa aacggtgcgg ctgaaacagc attactaggt
    19741 ttttatccgg ccagcctgcg cgcgccgctt taatggtggc gtcaacttcc gtcgggtggt
    19801 ggccgtagtc atcgaccagc attgccgtac cgcttttacc attcactggc tccagcggga
    19861 attcaccgag gaaatcaaaa cggcgaccag tcccctggaa gctctccagc gcacgcaaaa
    19921 tagcctcgtc gtcaattccc tcttccgtag caaccgcaac cgcagctgcg gcgttcagcg
    19981 cgttatgacg gcctggcgca ttcagggtga cgcgcatcgg ctctttgtcc tggcgcagca
    20041 gcgtaaagtg cccctgcggg ccaatctgct gataatcttc tacacgcacg tcggcatctt
    20101 cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg taacaattcg cggatcaccg
    20161 gatcatcaac acacatcacc gcacgaccgt aaaacggcag gttgtgcaga aaattaataa
    20221 aagtctgttt taaattctca aagtcgccct ggtaggtatc catgtggtcg gcttcgatat
    20281 tggtgacaat cgccaccatc ggttgcagat gcaggaacga tgcatcactc tcatctgctt
    20341 cggcaatcag gtaccgacca tgccccaaac gcgcatgaac ccccgccgct tttaccagcc
    20401 cgccgttaac gaaggttggg tcgagccccg cttctgcgta gatgctggaa accatcgcgg
    20461 tggttgtcgt tttgccgtgc gttcctgcaa tggcgatgcc atgacgaaaa cgcattaact
    20521 cagccagcat ttcggcacga cggatcaccg gaatacgcgc ttcatgagct gcgacaattt
    20581 ccgggttatc ggcagaaatg gcgctggaaa caacgaccac gctggcatca cgtacgtttt
    20641 ccgggcgatg gttgaaataa atcgtcgctc ccagattcat taactgctgc gtgaccggat
    20701 ttggcgctaa atcggaacca ctgatctgat aaccttcatt ggccagaact tcggcaatac
    20761 cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg ccgaacgcga cgcatttcgg
    20821 gcacgatgga acgcagtttt gccaattgtt gtgtattcat tctttacgcc attaacttct
    20881 taaattcata cgatgcaaaa ggcatcgcta caattacgcc cgggcagccc ggctcacttc
    20941 atttgccact cgctcggtgg catccggaat ggatgcagcg cgggcgcgtt ctgccatggt
    21001 taataaggtt tctcgcgacc acccggccag ggtgttggcg acagcatcca cgctaagctg
    21061 tggctgctcg ataattttgg ctgcgcccgc tttttccagc ggtagcgcat tccagtattg
    21121 ctggcggtct ttatgttgaa acggcacaaa caacgccggt agtcctgccg cggcgatttc
    21181 actcaccgtt aaagcaccgg agcggcaaac gacgacatcc gcccacgcat acgccgccgc
    21241 catatcatca ataaattccg tcactttatg ctgcggttgc cccgcttcgg catacgcctg
    21301 ttcaacggat tgttgcgaac ctttaccgct ctgatgccag atagtgactg aatcacccag
    21361 tttcgcagca acctgcggca ttgtctggtt aagaatgcgt gcgccctggg aaccacccac
    21421 taccagcaca cgaaccggac cttcacgtcc agccaaacgt tgctgcggca acggcagcgc
    21481 cagcacatcg gtacgcaccg ggttacccac cacttccgca ttagggaaag cgcctggaaa
    21541 cgcctgcatc actttggtgg caatcttcgc cagccattta ttggttaagc ccgcaatacc
    21601 gttttgttca tgaagtacaa ccggaatgcc taacgaccac gcggccagac cacctggacc
    21661 tgatacatag ccgcccatac cgagtaccac gtcaggtttg tacgctttca taatcgcccg
    21721 cgcctgacgc caggcgttga agatacgcag cggcgcagct atcagtgctt ttataccttt
    21781 tccacgcaga ccagagatac gaatgaaatc aatttcgatg ccatgttttg gcactaagtc
    21841 cgcttccata cggtcggcag tccccagcca gcgaacttgc caaccctgag ccattagatg
    21901 gtgcgcaacc gccagtcccg ggaatacatg tccaccggtt ccgcctgcca tcaccattaa
    21961 tcgctttcct tgaccactca tcgtgaacct cgtacaaacg cctgcgcttt ctccagacgc
    22021 gtttcataat caatacgcaa cagcatcatg atggctgtcg acataatcag taagctcgaa
    22081 ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg gtaacatccc cgccgccgcg
    22141 cctacgttaa ccagcgcctg gaagctaaac cagatgccaa tagaacaggc gagaaaaccg
    22201 gaaaaacggt ggtcaatttc taacgctttg cggccaatcg acatcgcgcg aaaagcgacg
    22261 aagaatacca ttaaaagtgc cagcaccaca ccgacatacc ccagttcttc gccgataatg
    22321 gcgaaaataa agtcagtgtg cgcttccggc agatactcca gtttttgtac cgagttacct
    22381 aaaccttgtc cccaaagttc gccgcgacca aacgccatca gcgattgcgt taactgatag
    22441 ccgctgccaa agggatcttc ccacgggttc cagaatgcgg taacacggcg gatacggtac
    22501 ggttcggcga gtatcagcaa cacaaccgct gaaatgccca taccgataat ggcaatgaac
    22561 tgccacaatt tcgctcccgc caggaacaac atcgccagcg tagtcacaaa caacaccacc
    22621 accgtaccaa ggtctggctg tgccagcagt aacactgcca acaccagaat cacgcccatc
    22681 ggtttcagga agccgcgcag gttattacgt acttcgtcgc ctttacgcac cagatagttg
    22741 gcgatataac aaaacagcga cagttttgtc agctccgcag gctggatacg cagcaaaccg
    22801 agatcgatcc aacgcgatgc ccctttaacc gagctaccca ctaccagcac gatcatcagc
    22861 aggatgatag atccgagcag catcgtggca ctgtagcgtt gccagaactc catcggcaga
    22921 cgcagcgtaa tgatcgccag aatgaatgcc aggatcagat agacgccatc acgcttcgcg
    22981 aagaagaacg gatcgttggt taagcgttgc cctatgggca ttgacgccga ggtcaccatg
    23041 ataaagccaa tcgccgcaag gccgaaggtc agccacagta aggtgcgatc gtacatgatc
    23101 aggctgtcgg tatctttttc ccgcgagccc atcacccagc cttttagcgc cgtggagatc
    23161 cagaccagga tactgaatcc tggcaggcgc ggcattttca ggcgagggag agataaacgc
    23221 atcaacctaa ctccttcgcc agacgggcaa actcattgcc tcgttgttca aagttcttga
    23281 actgatcaag gctggcacag gccggggaga gcagaaccat atcgcccagc tgaacacgcg
    23341 gagccagcaa gcgcatcgct tgttccatgg tttcggtttg ttctgccact tccgggcgta
    23401 gcgccgccag ctgcgcaccg tcacgaccaa aacagtacaa acgcacgttg tcgccattca
    23461 gatagcgcgc cagtggactg aagtccgccg atttaccatc gccgcccagc aacaaatgca
    23521 gcgtgccgtc tacctgcaag ccattcaacg ccgcttccgt actgccgacg ttagtggctt
    23581 tcgaatcgtt aatccagcgt acgccattgt gctccagcac cacttcaaag cgatgcggca
    23641 gaccggtgaa ggtggttaac gctttcaggc tgctggcgcg cggcaaacca gctgcgtctg
    23701 ccagtgccag tgctgccagc gcattggtgt agttatgctg cccggaaagt ttcatctctt
    23761 tcacattcag cactttctcg cctttaaccc gcagccaggt ttcgccctgc tgatgattca
    23821 ggtgatagtc acccatgttg acgccaaagc tgacgcagcg ttcatcagca ccgcgaatcg
    23881 gcattgttaa ggcatcatca gcattgacga cacaaacttt cgcattttcg taaatacgca
    23941 attttgccgc acggtattgc tgcaaaccaa acggatagcg gtccatatga tcttcagtca
    24001 cgttcagaat ggtcgccgcc accgcctgta agctggaagt ggtttccagc tggaagctcg
    24061 acagttccag cacgtacagt tcgcactcag catcaagcag catcaacgct ggcaggccaa
    24121 tattgccacc cacaccaacg ttaacccccg ccgctttcgc catttcaccc actagcgtgg
    24181 tgaccgtgct tttgccgtta gaaccggtaa tcgccacaat cggtgcttgt gcttcgcgac
    24241 agaacagctc gatatcgcca acgatttcga ttccggcatc agcggcagcg cttaaggatg
    24301 gatgcgcctg tgcaataccg ggactggcga caatcagatc tgccgccatc agccattcat
    24361 cattcagact gcccgtgtgg cgttctacgg cttcgggtaa tttatccagg ccaggcggtg
    24421 tcatacgcgt atccataacg cgcggcgtca caccgcgagc gaggaaaaag tccacgcagg
    24481 aaagcccggt gaggcccagg ccgataatga cgacattttt accctgataa tcagccatga
    24541 ttaacgtacc ttcagcgttg ccagaccaat cagaaccagc atcagcgaaa taatccagaa
    24601 acgcacaatg acgcgcggtt ccggccagcc tttcagttca tagtggtgat gaatcggtgc
    24661 catgcggaaa atacgttgtc cgcgcagttt aaaggagccg acctgcagga tgacagaaag
    24721 cgtttctacc acgaacacgc cccccataat caccagcagg aattcctgac gtagcagtac
    24781 ggcgataatg cctaacgcac cacctaacgc cagcgaacct acatcgccca taaagacctg
    24841 cgccggatag gtgttaaacc acaggaagcc cagtcctgcc ccgactatcg ccgtgcagac
    24901 aatgaccagt tcccctgcgt gtcgcagata cggtatatgc aagtagctgg caaagttcat
    24961 attgccggtc gcccacgcca ccagcgcaaa accaccggcg acaaatacgg tcggcataat
    25021 tgccaggccg tcgagaccat cggtcaggtt taccgcgttg ccagtaccca caatgacgaa
    25081 gtaagccagc agaatgtaga acagccccag ctgcggcatc acatctttaa agaatgggac
    25141 caccagctgc gttgcgggcg tgtctttgcc ggcaaggtac agggcgaagg cgacacccag
    25201 cgcaatgacc gacatccaga aatacttcca acgagcgatc aaccctttgg tgtctttacg
    25261 caccactttg cgataatcat caacaaagcc aataacaccg taacctacca gcaccaccaa
    25321 cacgcaccag acgtacggat tggacgggta agcccacagc agtacggaga tcacaatcgc
    25381 cgtcaggatc ataatcccgc ccatggtcgg cgtaccgcgc ttgctgaagt gtgattcagg
    25441 accgtcgtta cgcaccacct gaccaaagga aagtttttgc aaatgagcta tcatacgcgg
    25501 gcccatccac aatgagatga acagcgcggt cagcaggctg acgatggcgc gaaacgtcag
    25561 ataggaaaag acgttaaagc cggaataata tttgaccaaa tgttcggcca gccaaactaa
    25621 catgtcccat tctcctgtaa agcgcgtact acctcttcca tggcggcact acgtgaaccc
    25681 ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg atttaagacg cgtaattaac
    25741 gcagttttat cagcaaaatg ttcgccaacg ccgctggcgg tgctgatagc atggctttgt
    25801 ttacccacgc ttaacacgcg gtcaatacca gcagcttttg ccgcctcgcc cacctgtaca
    25861 tggcaggctt cgctttcagc gcccagttcc gccatatcgc ccaccaccag cacgcggtag
    25921 cccggcattt cagccagtac ctgaacagcg gcggtcattg aaccgacatt ggcgttgtag
    25981 gagtcgtcga gcagcaactg gttttctgcc agttggatgg ggaacagacg gcctggaaca
    26041 gctttcagat ttgccagccc cgctttgata gcatcaagcg ttgcgcccac ggacatggag
    26101 agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc ccggcaacgg cagcagaaca
    26161 tcgacgctac cggttggggt ttgtagggta aattccgtac cgtgcgaggt cacatggata
    26221 ttggtggcgg tgaaatcgct gttggcggca ttgggtgaga aacgccacac tttgcgtgag
    26281 ccaattacgc tctgccagtt cagccagtcg ttgttgtcgg cgttcataat ggcgataccg
    26341 ttttccggca ggccgctaaa gatttcacct ttcgctttcg cgacacccgc aagcgagcca
    26401 aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg cagcttccgg gcgagtcaga
    26461 ctcacagtcc aggctatttc gccctgatgg ttcgcgccaa gttcaataac tgcgtaatcg
    26521 tattccggcg tcaagcgcaa caacgtcatc ggtacaccga tgtcgttgtt gagattgcct
    26581 gccgtataaa gcgtgttgcc gcactggctt aaaatcgccg ccgtcatctc tttaacggag
    26641 gttttgccgg aggaacctgt cagagcaacc acgcgcgccg gaacttgctg gcgaacccat
    26701 gcagccagtt caccaaacgc cagacgcgta tccttgacga ttaactgcgg caggtcgata
    26761 tccagcggac ggctgaccag tagtgcgccc gcgccgccag ctttcgcctg gtcggcaaaa
    26821 tcatgagcat cgaaacgttc gcctttcagg gcaacaaaca ggcagcccgg cgtcagtttt
    26881 cgggtatcag tggttacagc atcaagggtg atatctgcac cttgcagttc accgttgaga
    26941 atgtcggtaa gttggctaag ggttacgcta atcatgcaat cacccccagc agacgcgcca
    27001 ccgtgacgcg atcggagtag tccagacgct gattgccaac aatctggtaa tcttcatggc
    27061 ctttgcccgc gaccagtacc acatcattct ctttagcctg cataacggcg caagtcaccg
    27121 cttcagcacg gccttccatc actttggcat gtccggcatc taacattccc gccagaatat
    27181 cgttgatgat ggcacgcggt tcttcggtac gcgggttatc gtccgtcacc accgccacgt
    27241 cagcaaactc ttcggcaatt gcgcccatca gtggacgctt acctttatcg cgatcgccac
    27301 cacagccaaa gacgcaccac agcttgcccg cacagtgcag gcgcgccgcc tgtaaggctt
    27361 tttccagtgc atccggcgta tgcgcgtaat ccaccaccac cgtcggtttg cctggcgcag
    27421 tgaacacttc catacgtccg caaaccggtt gcagacgcgc ggcggttttc agcagatcag
    27481 ccagtggata gccgagtgcc agcagtgtcg ccagcgcgag cagcaggttg ctgacgttaa
    27541 aagcgcccat cagatggctt tcaatttcgc catcgcccca acttgagcta aagcgaatcg
    27601 tcgcaccgct gtcgtgatag ttcacgtcga tcgcttttaa ccagcgtccg tgacagttcg
    27661 gattaatatg atcttccatt gataccgcaa ccgcgtccgg cagttttgcc agccagcggc
    27721 ggcccacttc atcgtcggcg ttaataatcg cctgaccgca atgatgttca gaataaagca
    27781 gccatttcgc agcttcgtag tgttccatat caccatgata atcaaggtga tcgcggctta
    27841 agttggtaaa gaccgacgcc gcaaatttca atgccgccac acggtgctgt accagcccgt
    27901 gggaggaaac ttccattgcg caaaacgtcg cgccctgctc caccagcccc gccagctcat
    27961 gctgaacatc gactgcggaa ccggtagtat tttctgtcgg gatcactttc ccaagcagac
    28021 cgttaccaac ggtgcccatt accgcgctgg tttcgccaag cagttggctc cactgcgcca
    28081 gcagctgggt agtcgtggtt ttgccgttgg tgcccgttac gcctacgaga cgtaaattgt
    28141 cagagggttc atggtaaaag cggcccgcca gtgcagataa acgctcattg agctggctga
    28201 gatagatgac cggtacgccg tgcatttcac ggatttcacc gtcggtcgcc tcatctttcg
    28261 ctttcgcctc tgcaataatg gcagccacac cttgcgctat cgcctgcggg atatatcgac
    28321 gcccgtccgc ctgatgacct actacagcta caaagagatc gcccgccgca gccacacggc
    28381 tgtcgagtgt catctctcgc agtgctcgcg aaggtgcgtc tggcacccac ggagcaagaa
    28441 ggtcgcgcaa attacgatct gccacctgtc ccctcgcctt gattaatcac aaattcattt
    28501 ttatcgcccg ttgtcagcgc atccggctcg atgttcatgg tacgcaatac gccgcccatg
    28561 atggcaccaa agaccggcgc ggaaacggcg ccgccgtagt atttacccgc ctgcggatcg
    28621 ttgataacaa caaccagcgc gaagcgcggc tgactcgcag gcgcaacgcc tgcggtataa
    28681 gcaatatatt tattgatgta gcgaccgtcc ggcccgacct ttttcgcggt accggtttta
    28741 atggcgatac gatagccttt aatcgccgcc ttcacgccgc cgccgcctgg tagcgccacg
    28801 ctttccatca tatgcaccac agtgcggaca atggatcccg ggaagacacg ttcaccggga
    28861 accggggggt caactttggt aatcgacagt gggcgataaa tgccgtagct gccgatagtt
    28921 gcgtagactc gcgctaactg taacggtgtt accattagcc cgtagccgaa agagaaggtg
    28981 gccctctcta tgtcagacca ccgttgtttt tgaggatata agccactgcg ttctccgacc
    29041 aaccccaaat tggtcgcttt tcccagtcca aaacgtgagt aagtatctac taacgctgag
    29101 gacggcatcg ctaacgccag cttggaaaca ccgacgttac tcgacttctg taataccccg
    29161 gtcagggtta attcgctgta gcgtgccacg tctttgattt cgtggccgtt aattcgataa
    29221 ggaatggtat tgagtaccga gttttcccgc accacgccac gttgcaacgc ggtcattacc
    29281 accatcggtt taaccgttga gcccggttca aacacgtcgg tgatggtacg gttacgcatc
    29341 gcctctttcg gcgtgccgct cagattgtta gggttgtatg acgggctgtt agccatcgcc
    29401 agcacttcac cggtgttgac atccaccagc acggcgctac cagattcagc cttgttaaag
    29461 gccaccgcgt tgttcagttc gcgataaacc agcgcctgca ggcgttcatc aatactcagc
    29521 gccaggttgt gcgctgcctg gctgtcagta gaagaaatat cttcaattac gcgaccatag
    29581 cggtctttac gcacaatgcg ctcacccggc tgcccggtaa gccatttatc gaaactcttc
    29641 tcaacgccct caatcccttg actatcgacg ttagtaaagc cgatgaggtg agcagtcact
    29701 tcgccggacg gatagtaacg gcgagactct tcacgcagat gaatccccgg cagtttcagt
    29761 tttttgatgt agtccgccat gtcagggttc acctgacgcg ccagataaat aaaacgccct
    29821 ttcgggttgg cgttaatgcg ggctgaaagc tgatccagcg gaatattgag cgcgttagcc
    29881 agcgccttcc agcggtcacc gacgctgata ccgccagcgt catgcacttc tttcgggtca
    29941 gcccaaatcg cttttaccgg cacgctcacc gctaacgggc gaccagaacg gtcagtaatc
    30001 atgccgcggg aggtggaaac ttgctgaacg cgaagagaac gcatgtcgcc ctctttcacc
    30061 agcatatccg gggagataac ttgtaaccac gctacgcgtc cgagcagaaa agccagcgcc
    30121 aggagaatac agccgcataa caacgcaaaa cgccaactga taaagttggc atgttcttcc
    30181 tgacgttttg gtttctgcgt tttcgccgct gctttcatgc gtcgcgttta tccttatttt
    30241 tgcactacga tattttcttg tgacggatca acatgctgca tttgcagctt ttccgtggcg
    30301 atcctttcca cccggctatg gtcgccgagc gcattctctt caaggatcag gttgcgccat
    30361 tcaatgtcta aagcatctcg ctccagcacc agttgttcgc gctgagcggt cagtaaacgg
    30421 gtatggtgcg ccgtggttac cacagtcacc gccgtcaaaa taatgcaaat gaacaggcag
    30481 agtggcagct tcccaaatcg caaaagatcg tcaccgataa caccaggcaa tgcatggcgc
    30541 tcgtggcttc ccatcgatcc tttaactttg cttagagctt ctgtcactct gctgatcatg
    30601 cattcgtcct ctctgcaata cgcagaactg aactacgggc acgagggttc tcagccacct
    30661 cttcttcgcc cggcattaac ttgcctagtg ctcgcagctg acggccaccc agttttttga
    30721 gctgctcttc agtcatcggt aaccctgccg gaacttgcgg accgcggctg ttttcacgca
    30781 taaaacgttt cacaatacgg tcttccagcg agtggaagct gatgatcgaa agccgcccac
    30841 ccggggccag cacgttgagc gagcttttta gcgcctgctc tatctcctcc agttcactgt
    30901 ttacccaaat gcgcaccgcc tggaaggtac gggtcgcggg atgtttaaac ttatctttca
    30961 ccggcgttgc aacagccacg acttccgcca gttctttggt gcgggtcatc ggctgttcgc
    31021 ggttacgctc gacaatggcg cgggcaatgc gtttggcaaa acgctcttca ccataggttt
    31081 tcaataccca agcgatatcg gcttcttctg cggtttgtag ccattcagcg gctgactgcc
    31141 cacgggttgg gtccatacgc atgtccagcg gaccatcgcg cataaaggaa aagccgcgct
    31201 cagcatcatc aagttgcggt gaagagacgc caagatcgag gagaatgccg tcgatcttgc
    31261 cgataagatc gcgctcggca acgtattcgc ccagcgcgga gaaaggtccg tggatgatgg
    31321 agaagcgcgg atcatcaata gtcttcgcaa cggcgatagc ctgcgggtcg cgatcgatcg
    31381 ccagcaaacg cccctcttcg ccaagctgcg agaggatcag acgtgagtga ccaccgcgac
    31441 caaaagtccc atcaatgtag atgccatcag gacggatatt gaggccatta acggcttcat
    31501 ccagcagcac cgtagtatgt ttatagtttt ccatcatttt atagagacaa gtcctgcagt
    31561 cgctccgata agtctccggt agccaactgc tctgcgtcga tatcttcctt gacctgttga
    31621 tgccaggttg tttcatccca cagctcaaac ttgttgaact gtccaaccag catcacttct
    31681 tttgtcagcc cggcatgttg ccgcagtact ggcgcgatta acaatcgacc tgcgccatcc
    31741 atctgacatt cgctggcatg acctaacagt aggcgctgca cacggcgctc aaccgggttc
    31801 atgctcgaca gacgcgataa tttttgctcg ataatttccc attcaggcag ggggtaaagc
    31861 agcaggcacg ggtgatgaat gtcaatggtg caaaccattt gaccgacagc gttctcaagc
    31921 agctgttccc ggtaacgggt aggcactgat aagcgccctt tgctgtcgag attgactaac
    31981 gttgctcccc ggaacatgcc agcctcaccc cttattccca ctttgcccca caatttccca
    32041 ctgaaaggag tttacggagc ggaggaaaag cttgtcaagc cagtcacaag gcataccgag
    32101 gtaaaaaacc cgatgtttac ggctaatatc agcgttgttt attttacgtc caacgtgcgc
    32161 agcaaattaa cgttatgaat agctgtaaga aaaaatccct catcgtcatc accgttttaa
    32221 aatgactcac tattttccac agcaaatata aagtgtcagt ttgcgacgcg agcggcattt
    32281 taggacatat cttccccggt taacagtccc tgttgcgtct gtgtggcgct tgatgccagc
    32341 gggtttgcgc aaaagcaaag aaaagtgctt gttaaatcgg caattcatct ctgcatgtaa
    32401 caaaataatg caaaaacgcg ctgtcgatat cacttaatta ttgtgaattt cagctggtga
    32461 gttaattaac gggcgggttt aaaagaactt atcaataata gtcaggaata gtcttattta
    32521 ctttaaggat attgatgtcc attcccgtac tctacg
//
LOCUS       DAZSBF010000024        32378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZSBF010000024 DAZSBF010000000
VERSION     DAZSBF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.024"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            137..700
                     /locus_tag="ACHXWD_000836"
     CDS             137..700
                     /locus_tag="ACHXWD_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619831.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            782..3424
                     /gene="yfcU"
                     /locus_tag="ACHXWD_000837"
     CDS             782..3424
                     /gene="yfcU"
                     /locus_tag="ACHXWD_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFR7619832.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3444..4195
                     /locus_tag="ACHXWD_000838"
                     /pseudo
     CDS             3444..4195
                     /locus_tag="ACHXWD_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4170..4718
                     /locus_tag="ACHXWD_000839"
     CDS             4170..4718
                     /locus_tag="ACHXWD_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619833.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4715..5185
                     /locus_tag="ACHXWD_000840"
     CDS             4715..5185
                     /locus_tag="ACHXWD_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619834.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5182..5706
                     /locus_tag="ACHXWD_000841"
     CDS             5182..5706
                     /locus_tag="ACHXWD_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619835.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5693..6565
                     /locus_tag="ACHXWD_000842"
     CDS             5693..6565
                     /locus_tag="ACHXWD_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR7619836.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6687..7238)
                     /gene="smrB"
                     /locus_tag="ACHXWD_000843"
     CDS             complement(6687..7238)
                     /gene="smrB"
                     /locus_tag="ACHXWD_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR7619837.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7404..8336
                     /gene="prmB"
                     /locus_tag="ACHXWD_000844"
     CDS             7404..8336
                     /gene="prmB"
                     /locus_tag="ACHXWD_000844"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR7619838.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8371..9456
                     /gene="aroC"
                     /locus_tag="ACHXWD_000845"
     CDS             8371..9456
                     /gene="aroC"
                     /locus_tag="ACHXWD_000845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR7619839.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9460..10284
                     /gene="mepA"
                     /locus_tag="ACHXWD_000846"
     CDS             9460..10284
                     /gene="mepA"
                     /locus_tag="ACHXWD_000846"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR7619840.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10284..11093
                     /locus_tag="ACHXWD_000847"
     CDS             10284..11093
                     /locus_tag="ACHXWD_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR7619841.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11093..11641
                     /gene="epmC"
                     /locus_tag="ACHXWD_000848"
     CDS             11093..11641
                     /gene="epmC"
                     /locus_tag="ACHXWD_000848"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR7619842.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11675..11953
                     /locus_tag="ACHXWD_000849"
     CDS             11675..11953
                     /locus_tag="ACHXWD_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR7619843.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(12074..14080)
                     /gene="mnmC"
                     /locus_tag="ACHXWD_000850"
     CDS             complement(12074..14080)
                     /gene="mnmC"
                     /locus_tag="ACHXWD_000850"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR7619844.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14239..15459
                     /gene="fabB"
                     /locus_tag="ACHXWD_000851"
     CDS             14239..15459
                     /gene="fabB"
                     /locus_tag="ACHXWD_000851"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR7619845.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15734..16912
                     /locus_tag="ACHXWD_000852"
     CDS             15734..16912
                     /locus_tag="ACHXWD_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7619846.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16909..17904)
                     /gene="flk"
                     /locus_tag="ACHXWD_000853"
     CDS             complement(16909..17904)
                     /gene="flk"
                     /locus_tag="ACHXWD_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR7619847.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            18003..19139
                     /gene="pdxB"
                     /locus_tag="ACHXWD_000854"
     CDS             18003..19139
                     /gene="pdxB"
                     /locus_tag="ACHXWD_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR7619848.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19205..20218
                     /locus_tag="ACHXWD_000855"
     CDS             19205..20218
                     /locus_tag="ACHXWD_000855"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR7619849.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20218..21030
                     /gene="truA"
                     /locus_tag="ACHXWD_000856"
     CDS             20218..21030
                     /gene="truA"
                     /locus_tag="ACHXWD_000856"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR7619850.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21113..21772
                     /locus_tag="ACHXWD_000857"
     CDS             21113..21772
                     /locus_tag="ACHXWD_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR7619851.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            21928..22842
                     /gene="accD"
                     /locus_tag="ACHXWD_000858"
     CDS             21928..22842
                     /gene="accD"
                     /locus_tag="ACHXWD_000858"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR7619852.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22912..24180
                     /gene="folC"
                     /locus_tag="ACHXWD_000859"
     CDS             22912..24180
                     /gene="folC"
                     /locus_tag="ACHXWD_000859"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR7619853.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24170..24832
                     /gene="dedD"
                     /locus_tag="ACHXWD_000860"
     CDS             24170..24832
                     /gene="dedD"
                     /locus_tag="ACHXWD_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR7619854.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            25091..25579
                     /gene="cvpA"
                     /locus_tag="ACHXWD_000861"
     CDS             25091..25579
                     /gene="cvpA"
                     /locus_tag="ACHXWD_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR7619855.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25616..27133
                     /gene="purF"
                     /locus_tag="ACHXWD_000862"
     CDS             25616..27133
                     /gene="purF"
                     /locus_tag="ACHXWD_000862"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR7619856.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27228..27797
                     /gene="ubiX"
                     /locus_tag="ACHXWD_000863"
     CDS             27228..27797
                     /gene="ubiX"
                     /locus_tag="ACHXWD_000863"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR7619857.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            28063..28845
                     /gene="argT"
                     /locus_tag="ACHXWD_000864"
     CDS             28063..28845
                     /gene="argT"
                     /locus_tag="ACHXWD_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR7619858.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            29066..29848
                     /gene="hisJ"
                     /locus_tag="ACHXWD_000865"
     CDS             29066..29848
                     /gene="hisJ"
                     /locus_tag="ACHXWD_000865"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR7619859.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            29938..30624
                     /gene="hisQ"
                     /locus_tag="ACHXWD_000866"
     CDS             29938..30624
                     /gene="hisQ"
                     /locus_tag="ACHXWD_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR7619860.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30621..31337
                     /locus_tag="ACHXWD_000867"
     CDS             30621..31337
                     /locus_tag="ACHXWD_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7619861.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31345..32118
                     /gene="hisP"
                     /locus_tag="ACHXWD_000868"
     CDS             31345..32118
                     /gene="hisP"
                     /locus_tag="ACHXWD_000868"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR7619862.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            32315..>32378
                     /locus_tag="ACHXWD_000869"
     CDS             32315..>32378
                     /locus_tag="ACHXWD_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7619863.1"
                     /translation="MTISTTSTPHDAVFKSFLRHP"
BASE COUNT         7591 a         8008 c         8749 g         8030 t
ORIGIN      
        1 cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca atggctgggt
       61 ataaacattg gttattgact gaatcaatgt attgcaatgt ttatattttt aattatttat
      121 agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg caatggtgat
      181 gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg cacgttttta
      241 cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac tggaagttga
      301 tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc cgaaaagttt
      361 ccagattcgt ctgcaagatt gcgtatttga tactcaggaa acaatgacca ctacctttac
      421 tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata ccgatacctg
      481 tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct cttacaaaag
      541 cggcatgggt attgaccaga aaatagtgaa agacacttct accaataaag gcaaagcgaa
      601 gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag atctgggtaa
      661 tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc catagcgaca
      721 ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga tagaaacgtg
      781 tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg cgctggcaat
      841 gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt cccgttttct
      901 ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg gatatgttga
      961 gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg aagagtacga
     1021 tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc tgacaccgga
     1081 actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat ggagccacga
     1141 tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg atttaagcca
     1201 gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc ccgactggga
     1261 tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca gcatcaacgc
     1321 acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg gcaacgggac
     1381 ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta actatcaaca
     1441 tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa aatgggagtg
     1501 gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg cgctgggcga
     1561 ggattacctc agatccgata tttttgatgg ttttaactat gttggtggca gtgtcagtac
     1621 tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt ccggcgtggc
     1681 acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg aaacgcaggt
     1741 tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta cgttgcatat
     1801 tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg cctcgatgcc
     1861 atacctcact cgtccaggtc aggttcgcta taagatcatg atgggccgtc cgcaagagtg
     1921 gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg ggatcgctaa
     1981 cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg cggcgcttgg
     2041 cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc actcgcacac
     2101 caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact ccttccgtgt
     2161 gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg gatatcgctt
     2221 ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca gcggaatggt
     2281 acgcacgggc aacgacaaag agatgtacac tgccacttat aaccagaact tccgcgatgc
     2341 gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg aggagcagac
     2401 aaactacaac atcatgctct cgcactattt caatataggc agtattcgta atgtcagcat
     2461 ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca tgtacatttc
     2521 gctcagtatg ccgtggggtg acaacagtac cgttagctat aacggtaact atggcagtgg
     2581 gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc actatcagtt
     2641 gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc atgatggttc
     2701 gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct ctgcgggctt
     2761 gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc gtacccagaa
     2821 tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc cggtagaagg
     2881 taacggtgct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg acgtcaataa
     2941 ctattaccgc aatcaggcgt atatcgacct caacaaactg ccggaaaacg ccgaagcaac
     3001 ccagtcggtg gtacaagcca cgctaactga aggggcgatt ggctaccgca aatttgccgt
     3061 catcagtggt caaaaagcga tggcggtgct gcgtttacaa gacggcagcc atcctccgtt
     3121 tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg atgacggcaa
     3181 tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga gtggtgttgc
     3241 gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg gcttgttact
     3301 gccatgccag cataaaggca acgtcgcgcc ggttgttcct gatgacataa agcctgtcat
     3361 tcaggagcag acgcaacagg tgacacccac caatccccct gtttctgttt cagctaacca
     3421 ataacgtgat taaggaatga tccatgtctg atttactttg ctctgccaaa ttgggtgcta
     3481 cgacgctagc gttactgctt agcgctgcca gtttgtcgcc caggcttctg taacgccaga
     3541 tcgtacccgt ttgatattta atgaaagcga caaatcaatc agcgtgacgt tgcgcaataa
     3601 cgatccgaaa ctgccttatc tggcccaaag ctggatcgaa gatgaaaagg gtaacaagat
     3661 cagttcaccg ttaactgttc tgccaccggt gcaacgcatc gattcaatga tgaacgggca
     3721 ggtaaaagtg cagggaatgc cggatatcaa caaattgcct gccgatcgtg aaagcctgtt
     3781 ctacttcaac gtgcgtgaga tcccgccaaa atcaaacaaa gccaacacgc tgcaaatagc
     3841 actacaaacg cgtattaagt tgttctggcg accgaaggcg ctggagaacg tcagcatgaa
     3901 aaacccctgg caatataagg ttactttaac ccgcaacggt caggaattta ccgtcaataa
     3961 cccgacgccg tattacgtca ttttcagtaa tgccagcaca cagaaaaatg gcaatcctgc
     4021 agccggtttc tcgccaatag tgatgtcgcc gaaaacctct gcaccgctga atgtgaagat
     4081 gggtagcgtg ccggtactga cctatgtcaa cgactatggc gctcggatgc cgctcttttt
     4141 cacttgcaac ggtaacagtt gccaggtcga tgaagagcag agccgcaaag gctgaggccg
     4201 ggagagccag tatgaaagga tcttgttctg tctgggcggt aactgggtta ctgatatgtt
     4261 gtgttggtag cggctttgca gttgcagcga gtaagtcagt aagtatgaat ttacgggtgc
     4321 ttgtcgatgc ccctcctcct tgtacggtaa atggtgcggc agttgagttt ggtaatgtat
     4381 tcattaataa gatcaatggg gttgattata aacgtcccat tgactactcg cttgtttgta
     4441 acaatctggc aatggatgac ctgcgtctac agatgcaggc gacaacggtc gtgattaacg
     4501 gtgaaacagt tatcagcacg ggtattcctg ggtttggtat tcgggttcag aaaagtagtg
     4561 atcacacaat actcgactta acctctggct catggttgcc atttaatttt tcctcgggag
     4621 ttcccgttct ggaagctgtg ccggtgaaac agagtggtac aacgttagcg gcagctgaat
     4681 ttaacgccag tgccacaatt gtggtggatt accaatgaaa cggatctcgc ttattttgct
     4741 gtggggcttt tgctcgatgg cactctcaaa cgtctctttt cacggttatt tagtgcagcc
     4801 gcctaactgc acgattagca acgctcaaac cattgaaata acttttcagg atgtgcttat
     4861 tgatgacatt aatggcagca attatgagca aaccgtgccg tattccatta cctgcgatac
     4921 ggcggttcgc gacccattaa tggaaatgac cttgagctgg agtggcacac taagcgattt
     4981 tgataatgct gcagtaagca gcaatatcac tggtttaggc attcagctca aacaggcagg
     5041 gcagagtttt acgatcaata cgccacttat ggtgaatgaa accgatttac cggtgttaac
     5101 ggctgtgcca gtaaaaaaga gtggtgtcgt tctgccagag gcggactttg aggcctgggc
     5161 cacgttacag gtagattacc aatgaagaaa aagcgtacgt tgttttttat tagtagcctg
     5221 atgttgctgg gcagtggaac cacaatagca ggagataatt tgcactttac cggcaattta
     5281 atcagtaaat cgtgtacgcc agtgattaat ggtagccagt tggcagaagt acattttcct
     5341 gccattgccg ccagcgattt aatgaacctt gggcagtcag agcgagtgcc attggtgttt
     5401 caattgaaag attgtcatag ttctacattg tttaatgtca aagtaacgtt gacaggaaca
     5461 gaagatagtg ctctacccgg ctttctggcc tttgactcct cgtcatcagc atccggcgct
     5521 gggattggta ttgaaactgc ggcaggcaca tcggtgccaa tcaacaatac aacaggcgtc
     5581 acgctcccgc tgaatcaggg aaataacagc ctaaatttta atacctggtt acaggcaaag
     5641 agcgggcgag atgtaacgtc cggagacttt agtgccacag tgacggcaac ctttgagtat
     5701 ttctgaaatg aaaaaatgga ccataatttt cgcgagccta atgctgctcg ttctgagtgt
     5761 tgtcggcgca tctaagagtt atgctggaga taaacttatg agtgcgagtt ttgacagtgt
     5821 gagagtttat tatgccatgg acaaggtaac cggtgctgta gcgagcagtg ttttcaatgt
     5881 tacggtgata acgccaaagg aggttacata tgggaaatat gattcttttg cgtataaagg
     5941 cgatacgttg cgggttattt cctggtctgg gtcgggttca gctccaactt tggtactgac
     6001 tgactttgat acaattaata actcaaattg ccctggcata gatacgaaaa tttttcgttg
     6061 tgcatatatg acgtttaaaa ttaccgttgc atctgatgat tatggttgcc cttggatcgc
     6121 atccttttat tcctatacag atttacccgg atttggaagc tatacggccc ctacagtgca
     6181 taacacaata tgcccaacga taccggtcac ttcatacgat atctcctgga gtgaaaatta
     6241 tgtcagccat aataaggcgt tacgaattca gtccacgggg tcaacagtga ccaccacgct
     6301 ttcgacctac ctgatggaag gcggcagact ctgtgatggc agtaattttt ctgataatga
     6361 tggcagagga gcatactgcc gcgcggtatc agaattgtta acctttacct cttacggttg
     6421 tgataaatca acggtaacgg ttacgccaac tcgtcatccg gtaacggata aagtactaca
     6481 cgatattgtg gtgaatgtga ataccagtag tgggcaaccg attgattcga cgtgtcgttt
     6541 tcagtatgta ttgaatgaac tgtagttcgt agtattgctc tgatgcgtct tatcaggcct
     6601 acgaagtgaa ttgcaattta ttgaattatc acatatttgt aggccggata aggcgtttac
     6661 gctgctccgg catttgctgc cagaattcag ggcaactccg gcggcaacca ctcttccact
     6721 tcaatcaaca ccaacaaagc cgcatcaccg ccgtactctt tcggtgcctg gtgaaatgcc
     6781 atcacatgcg gatgttgtgc cagccacagc ggtgtttgtt gcttcaaaat atgcttcccg
     6841 tgcccatgca tcacgcaggc acaaaacaca tgttcgcggc gacaggcggc aatcaacgcg
     6901 cccagttcct gcttggcctg cagctgcgtc agaccgtgta aatccaaaaa caattccggt
     6961 gaataatcgc cgcggcgcag tttcttcgcc tcaaaatggc tgacatccgg gcgaacatat
     7021 ttcaccggac cttcggtatt taataacggc tgaaactcat cggagaaata atggctggca
     7081 tcagcctgct ccgggatcaa gcgtttcacc ggcacttcgc tgattttttt acgctgcggt
     7141 cggtggacaa tcgtgtcctg cttaatcttg cgagtccccg ccatcaactg gcgaaacaga
     7201 gcctggtcct cctcgctaag tgttgttttc tttttcattt atctgtctct atttctaatt
     7261 ttcctacagt ttacccgact cgtagcacaa tcgatcgttt taaacgtatt tttcctcact
     7321 caccgcgcgt gaatgctgat ttatcgtcat cttcatggca aactagccgc cgaaattatt
     7381 gcgatcatgc cctggaggaa tacgtggata aaattttcgt tgatgaagca gtaaatgagc
     7441 tgcaaaccat tcaggacatg ttgcgctggt cggtaagccg cttcagcgcg gcaaatatct
     7501 ggtacggtca cggcaccgat aacccgtggg atgaagccgt acagctggtg ttaccatcgc
     7561 tctacctgcc gctggatatc ccggaagata tgcgcaccgc gcgtctgacc tccagcgaaa
     7621 aacaccgtat tgttgaacgc gtgatccgcc gcgtcaacga gcgcattccg gtggcttacc
     7681 tgaccaacaa agcctggttc tgcggtcatg aattttacgt cgatgaacgc gtgctggtgc
     7741 cgcgctcgcc gattggtgaa ctgatcaaca ataaatttgc cggactcatt agcaagcaac
     7801 cgcagcatat tttagatatg tgtaccggta gcggctgcat cgccattgcc tgtgcttatg
     7861 ccttcccgga agcggaagtc gacgcggtag atatctctcc cgacgcgctg gcggttgccg
     7921 aacagaacat cgaagaacac ggtctgatcc acaacgtcat tccgattcgt tccgatctgt
     7981 tccgtgattt gccgaaagtg cagtacgatc tgattgtcac taacccgccg tatgtcgacg
     8041 cggaagatat gtccgacctg ccaaacgaat accgtcacga gccggaactg ggcctggcat
     8101 ctggcaccga cggccttaaa ctgacgcgtc gcattcttgg aaacgcggca gattaccttg
     8161 ctgatgatgg cgtgttgatt tgtgaagtcg gcaacagcat ggtacatctt atggaacaat
     8221 atccggatgt tccgttcacc tggctggagt ttgataacgg cggcgatggt gtgtttatgc
     8281 tcaccaaaga gcagctgctg gcggcccgtg aatatttcgc gatttataaa gattaagtaa
     8341 acacgcaaac acaacaataa cggagccgtg atggctggaa acacaattgg acaactcttt
     8401 cgcgtaacca ccttcggcga atcgcatggg ctggcgctcg gctgcatcgt cgatggtgtt
     8461 ccgccaggca ttccgctgac ggaagcggac ctgcaacatg acctcgaccg tcgtcgccct
     8521 gggacatcgc gctataccac ccagcgccgc gagccggatc aggtcaaaat tctctccggt
     8581 gtttttgaag gcgttactac tggcaccagc attggcttgt tgatcgaaaa cactgaccag
     8641 cgctctcagg attacagcgc aattaaagac gttttccgcc caggccatgc tgattacacc
     8701 tacgaacaaa aatacggtct gcgcgattat cgcggcggtg gacgttcttc cgcccgcgaa
     8761 accgccatgc gcgtggcggc aggggcgatt gccaaaaaat atctcgccga gaaatttggt
     8821 attgaaatcc gcggctgctt gacccagatg ggcgacattc cgctggaaat caaagactgg
     8881 tcgctggttg aacaaaatcc gttcttctgc ccggacccgg acaaaatcga cgcgttagat
     8941 gaactgatgc gcgcgctgaa aaaagagggc gactccatcg gcgcgaaagt caccgttgtt
     9001 gccagtggtg tccccgccgg acttggcgag ccggtctttg accgcctgga tgccgacatc
     9061 gcccatgcgc tgatgagcat caacgcggtg aaaggcgtgg aaattggcga cggttttgac
     9121 gtagtggcgc tgcgcggcag ccagaaccgc gacgaaatca ccaaagacgg tttccagagc
     9181 aaccatgcgg gcggcattct cggcggtatc agcagcgggc agcaaatcat tgctcatatg
     9241 gcgctgaaac cgacctccag cattaccgtg ccggggcgca ctattaaccg ctttggcgaa
     9301 gaagttgaga tgatcaccaa aggccgtcac gatccctgtg tcgggatccg tgcagtgccg
     9361 atcgctgaag cgatgctggc gatcgtttta atggatcacc tgttacggca gcgggcgcaa
     9421 aatgccgatg tgaagactga tattccacgc tggtaaaaaa tgaacaaaac cgcgattgcg
     9481 ctgctggctc tgcttgccag tagcgctagc ctggcagcga cgccgtggca aaaaataacc
     9541 caacctgtgc cgggtagcgc acaatcgata ggcagttttt ctaatggctg tattgtcggc
     9601 gctgacacgt tgccgataca gtccgaacat tatcaggtca tgcgtaccga tcagcgtcgc
     9661 tatttcggtc acccggatct ggtgatgttt atccagcgtc tgagtagcca ggtgagcaat
     9721 ctgggcatgg gtacggtgct gattggcgat atggggatgc ccgctggtgg gcgtttcaac
     9781 ggcggtcatg ccagccacca gaccggactg gatgtcgata tctttctgca actgccgaaa
     9841 actcgctgga cctccgcgca gctcttgcgc ccgcaagcac tggacttagt ttcccgcgac
     9901 ggtaaacacg ttgtctccac gctgtggaag ccagaaattt tcagcttgat caaactcgct
     9961 gcccaggaca aagacgtcac gcgcattttt gttaatccgg cgattaaaca acaactttgc
    10021 cttgatgcgg gcaccgatcg cgactggttg cgcaaagtgc gaccctggtt ccagcatcgc
    10081 gcgcatatgc atgtacgatt acgttgccct gccgatagtc tggagtgtga agatcaacct
    10141 ttaccgccat caggcgatgg ttgcggggca gaactgcaaa gctggtttgc acctctaaaa
    10201 ccgggaacaa caaagcctga gaagaagaca ccgcctccgt tgccgccttc ctgccaggcg
    10261 ctactggatg agcacgtgat ctaatggaaa cgtttaatag cctgtttatg gtttccccgc
    10321 tgttgctggg agttctcttt tttgtcgcca tgctggcggg atttatcgac tcgattgccg
    10381 gtggcggtgg gttactcacc attccggcat tgatggctgc gggaatgtct cccgctaacg
    10441 cgctggcaac caataaactg caagcctgcg gcggctctat ttccgccact atctacttta
    10501 ttcgccgcaa agtggttagt ttaagcgatc agaaactcaa tattgccatg acctttgtcg
    10561 gctcaatgag cggcgcactg ttggtacagt acgttcaggc tgatgtctta cggcagattt
    10621 tgcccattct ggtgatttgt atcggcctct atttcttgct gatgccaaag ctgggtgaag
    10681 aagatcgcca gcgccggatg tacggtttac ccttcgcgtt aattgccggt ggctgcgtcg
    10741 gtttttatga cggattcttt ggcccggctg ccggatcgtt ttacgcgctg gccttcgtta
    10801 cgctgtgcgg attcaacctc gccaaagcca cggctcacgc caaattactc aacgcaacgt
    10861 caaacattgg cggtctgctg ctgtttattc tcggcggcaa agtgatttgg gcgacgggct
    10921 ttgtcatgct ggtggggcag ttcctcggcg cgcgcatggg ttcacggctg gtgttgagca
    10981 aaggacaaaa gctgatccgc ccgatgattg ttatcgtctc ggcggtgatg agtgccaaat
    11041 tactttacga tagccacgga caggagatcc tccactggtt ggggatgaac taatgaacag
    11101 tacacaccac tatgagcagt tgattgaaat ttttaatagc tgctttgccg atgaatttaa
    11161 tacccgtctg attaaaggcg acgacgaacc gatctatctt cctgctgatg cggaagtacc
    11221 gtataaccga attgtctttg cccacggctt ttatgccagc gcaattcatg agatttcgca
    11281 ctggtgtatt gccggaaaag cgcgccgtga gctggttgat ttcggctact ggtattgccc
    11341 ggatgggcgt gatgcccaaa cgcaaagcca gtttgaagat gttgaagtga agccgcaggc
    11401 actcgactgg ttgttctgtg tggcggcggg atatccgttt aacgtcagtt gcgacaatct
    11461 ggaaggcgac ttcgaaccgg atcgtgttgt gtttcaacgg cgggtacatg cgcaggtgat
    11521 ggattatctg acaaatggca ttcccgagcg tccggcacgc ttcattaaag cactacaaaa
    11581 ttattatcac acgccggaac ttacggcgga acagttcccg tggccggaag cgctcaactg
    11641 aagccggtgc cacgttttac aatgaggaaa gaagatgatc gcggagtttg aatcacgcat
    11701 tctggcatta atcgacggca tggttgacca tgccagtgat gatgagttgt ttgccagtgg
    11761 gtatttgcgt ggccacctga cattagccat cgcagaactg gaaagtggtg atgaccactc
    11821 cgctcaggcg gtgcatacga ccgttagcca gagtctggaa aaagccattg gtgccggtga
    11881 attgtcgccg cgtgaccagg cgctggtgac cgatatgtgg gaaaacctgt ttcagcaggc
    11941 gtcacagcag taagttgcgg cgtctggagc accatgcctg atgcgacgct tatcgcgtct
    12001 tatcaggcct acacgtcaca cgtaggtcgg ataaggcgtt cacgccgcat ccggcaaaca
    12061 ctgcgagagc agattacccc gccttaaccg ctttaccctt caacaatttc cgcacccata
    12121 accgattcgg gtttaacgcc gtcagcgtac tggcatccat cggaatcggt tcgtcgctca
    12181 tctgcgccgc cagaatctcg gcacacagcg gggcagaaca caaaccgcga gaacctaaag
    12241 ccgcaaacat aaagagatcg tcaaaaaccg gcgcgcttac cgcctcatct ttctgctccg
    12301 ccaacgacgc atattccacg agtgttgcct cataatcggg aacattgcct accattggca
    12361 gatgatcgcg ggtggcacaa cgcacaccgc agcgcgcctc tttatcactg acatcaacct
    12421 cttttgccca ctgtgcctgc gggaaacaat caatcaaccg ctggcgattc tgctgctgat
    12481 cgtcctcact gtacgccgta tcttcgctgc cgcgatgata actggcacca atacaatgat
    12541 gttgattcgc cggattttgt ggcgtgagat aaccgtcata gcacagcacc tgcttcagct
    12601 ctgccagttc cggcgttgtc ggaatatggc tgacctgccc ggcaaccgaa tacaccggga
    12661 gagtcgacgt ttggctgaaa cggctgattt gatgcccgtt cgccagtacc actacgctgt
    12721 gtgttgcttg ctgatctcct gcaaaattca acaaccaaca gtcatcctta cgggataaat
    12781 tctgtaactg atattgataa taaatctgca aaccctgctg ttgcgccagt tccagcacat
    12841 tacgggtcag ttctgctggg cacagccaac caccttgcgg ataagtaatg ccgctgcaat
    12901 ttgtcgcaac gcccgtaatt tgttcaaccg catttgcctc aacggctaca gccagttctg
    12961 cgggtaaatc cattgacaac atctgtgcga ttttatgctg gcttttctca tcccagccta
    13021 actgcgtgac gccgcaccag tcatgatcaa atttaacggg taattggtcg taaaaccgac
    13081 gagcaaaagt aaacgcatta gagaaaaagc ggtttagcgc ctcatcgtgt ttgcttaata
    13141 acggatacag cgccccctgg cgattgccgg aagcacccag cgcgggggct tcatccgcgc
    13201 aataaagcgc tacctgccag ccgcgccgta ataacgccag cgacaacaac gcgctggcaa
    13261 taccaccgcc gataatcgct acttcccgtt tgctgctgcc cgtacgatta aaccacggcg
    13321 tggagcaggg gagtggtaat gtctgttcca tcaccccaca aagcatttcc cgtttgcgcc
    13381 caaagccctt acgtttttgc atcgtgaatc cggcttcctg caaaccgcgg cggacaaaac
    13441 cggcagacgt aaatgtcgcc agcgtgccgc ctggacgcgc caaccttgcc atggcgttaa
    13501 acagattttg cgtccacata tccgggtttt tagctggcgc aaagccgtcc agaaaccagg
    13561 catctacttt ttgatttagc gaatcgtcca gttggctggt cagttcgtta atatcgccaa
    13621 accataaatc cagcgtcacg cggccttcat tgagcaataa acgatggcaa ccgggcaagg
    13681 gcattggcca ctgcgcctga agttgttctg cccacggagc cagttccggc cagtgttgat
    13741 gcgctaaggc taaatccgca cgggtgaggg gaaatttctc aaaactaatg aaatgtaagc
    13801 gttgtaattg cgcttgcgga tgcgcttcgc gaaactgatc aaatgcctgc catagcgtca
    13861 ggaagtttaa tccggtgccg aagccgctct ctgctaccac aaacagagga tgtggatgct
    13921 caggaaagcg tacctctaat tggttgcctc ccagaaaaac ataacgcgtc tcttccagcc
    13981 cgttatcgtt ggaaaaatag acatcgtcaa aatctcggga aacaggtgta ccctcagcat
    14041 taaattcgag gttggcaggt tgtatggagt agtgtttcac gtaagttact cgtcttacag
    14101 gcggtggctc gatcttagcg atgtgtgtaa ggctgcgcaa atttctctat taaatggctg
    14161 atcggacttg ttcggcgtac aagtgtacgc tattgtgcat tcgaaactta ctctatgtgc
    14221 gacttacaga ggtattgaat gaaacgtgca gtgattactg gcctgggcat tgtttccagc
    14281 atcggtaata accagcagga agtcctggca tctctgcgtg aaggacgttc agggatcact
    14341 ttctctcagg agctgaagga ttccggcatg cgtagccacg tctggggcaa cgtaaaactg
    14401 gataccactg gcctcattga ccgcaaagtt gtgcgcttta tgagcgacgc atccatttat
    14461 gcattccttt ctatggagca ggcaatcgca gatgcgggcc tttctccgga agcttaccag
    14521 aataacccgc gcgttggcct gattgcaggt tctggcggcg gttccccgcg tttccaggta
    14581 ttcggcgctg acgcaatgcg cggcccgcgc ggcctgaaag cggttggccc gtatgtggtc
    14641 accaaagcga tggcatccgg cgtttctgcc tgcctcgcca cgccgtttaa aattcatggc
    14701 gttaactact ccatcagctc cgcgtgtgcg acttccgcac actgtatcgg taacgcagta
    14761 gagcagatcc aactgggcaa acaggacatc gtgtttgctg gcggcggcga agagctgtgc
    14821 tgggaaatgg cctgcgaatt cgacgcgatg ggtgcgctgt ctactaaata caacgacacc
    14881 ccggaaaaag cctcccgtac ttacgacgct caccgtgacg gtttcgttat cgctggcggc
    14941 ggcggtatgg tagtggttga agagctggaa cacgcgctgg cgcgtggtgc tcacatctat
    15001 gctgaaatcg ttggctacgg cgcaacctct gatggtgcag acatggttgc tccgtctggc
    15061 gaaggcgcag tacgttgtat gcagatggcg atgcatggcg ttgatacccc aatcgattac
    15121 ctgaactctc acggtacttc gactccggtt ggcgacgtga aagagctggc agctatccgt
    15181 gaagtgttcg gcgataagag cccggcgatt tctgcaacca aagccatgac cggtcactca
    15241 ctgggcgctg cgggtgtgca ggaagctatc tactctctgc tgatgctgga acacggcttt
    15301 atcgccccga gcatcaacat tgaagagctg gacgagcagg ctgcgggtct gaacatcgtg
    15361 accgaaacga ccgatcgcga actgaccact gttatgtcta acagcttcgg cttcggcggc
    15421 accaacgcca cgctggtaat gcgcaagctg aaagattaat tcgcagtagg ccggagtagg
    15481 tcggataaga cgcgccagcg tcgcatccga cgttacgcgc caatgcggcc tccggcacta
    15541 acgcaaaagg gaacctgatg gttccctttt tcacatcatt gacaatcgcc accagttcca
    15601 ggcaaacttc ccgctttgtc gatttccttc tgaaaagacg tacgcgttaa atcctgccaa
    15661 cgcaccgtaa ccctgaaacc agagagatga gacggggata ctcctcgcct tgcgctgcat
    15721 tctggagtaa tgcatgactg ctgtaagcca aaccgaaaca cgatcttctg ccaatttttc
    15781 gctcttccgc atcgcttttg cggtttttct cacctacatg accgtagggc tgccgttgcc
    15841 ggttatcccg ctgtttgttc atcatgaact gggctatggc aataccatgg tcggcattgc
    15901 cgtcgggatt cagtttctgg ctacggtgct gacgcgtggc tatgccgggc gactggccga
    15961 tcaatatggc gcaaaacgtt cggcgcttca ggggatgtta gcttgtggtc tggctggcgg
    16021 cgcgttgctg ctggcggcga ttttgcctgt ctccgcaccg ttcaaatttg ccctgttggt
    16081 catcgggcgt ttgattcttg ggtttggtga aagccagtta ctgacaggcg ctctgacctg
    16141 ggggttaggc atcgtagggc caaaacactc tggcaaagtg atgtcatgga atggaatggc
    16201 gatttacggt gccctcgctg ttggtgctcc gcttggcctg ttgattcata gccattacgg
    16261 ttttgccgca ctggcgatca ccacaatggt attaccctta ctggcgtggg cctgtaacgg
    16321 cacagtgcgc aaagtaccgg ccctggcggg agaacgtcca tcgctgtgga gcgttgtcgg
    16381 gtttatctgg aaaccagggt tagggctggc actacaaggc gttggttttg cggttatcgg
    16441 gactttcgtt tcgctctact ttgccagcaa aggatgggcg atggcgggct ttactcttac
    16501 cgagtttggc ggcgcatttg tcgtgatgcg cgtcatgttt ggctggatgc cggaccgttt
    16561 tggcggcgtg aaagtggcga ttgtctctct gcttgtagaa acggtgggct tgttgctgct
    16621 ctggcaagcc ccaggtgcgt gggtcgcatt agcgggcgcg gcgttaaccg gagccggatg
    16681 ttcgcttatc tttcctgcgc tgggcgtgga ggtggttaaa cgcgtcccct cacaagttcg
    16741 cggcaccgca ctgggcggtt acgccgcgtt tcaggatatc gccctcggcg tctccgggcc
    16801 gctggcggga atgctggcga ccacgtttgg ttactcttcg gtatttcttg ccggggcgat
    16861 ctctgcggtg ctgggaatta ttgtcacgat actgtcattt cgtcggggct aacgaaccag
    16921 ccagaccagc atcaggataa tcgcgactaa cagtatccac agcgcgggac gcgttgccat
    16981 attttgcaac gtgtccatca tcggtggaaa aggattaagc agcggttgca tatcacgcgg
    17041 atcaatccct ttaacccgcc gttgataaag ctgattaaga atatcctcgg cctgggcaga
    17101 agaaagcgat gattgcgcag aaagaccata tttttgctgg atatacgccg ataacgctgc
    17161 cagttcactg gcatctaaag gttgttttag cgccatctgt agtgattcca gcgtcggcgt
    17221 attttgctgg cttaacgtct ggcgcgcctg caaccaggtc accagatggt taaacagttt
    17281 cgctggaatt aactcgccat cgttcacccc ggaaagttcc agcatcgatt gccagatctg
    17341 tttgctgggt tcccccgttg ccgccgcaag tttggtcacc agctgtttta gcgcattgtg
    17401 ctccgccggt aataaaggac ggtcggtcgc ctcgcgctgc tgtggttgcg gaataaccat
    17461 cttcccttcc tgcaacaggg tgagaatggt ttttaattgc tccggtgaga gctgattcag
    17521 cggcgtctga ccaaagttat gacggatata atccgtgacc gcctgacgat tattccccag
    17581 acgtaaatac tcccctaatt gcgctaaaag ctggcgggca gaatggcttt tttgcgcggc
    17641 cagcagacgt tgcgccagat tatgctcagc ggcagggaag tgacgcgaaa gcagcggtga
    17701 atctcccgac agaccaatat cgtgcctgat acccgcccac agttctgctc tttgttgttg
    17761 cgtcagtgag gtcactttcg tcattaagct ttccagcgaa gtacgttgct gactggataa
    17821 aggctgattg cccgcgccag acggcaggtt atctccctga cctggtggtt gcccaggagg
    17881 agggccggaa ataggttgta tcattacgta tccttatacc tgaaatcttc gcaagtatgc
    17941 ctggccgcga gattatggca cacttgtccg gttaactctc gtctcataca ggtaacacaa
    18001 acgtgaaaat ccttgttgat gaaaatatgc cttatgcccg cgacttattt agccgtttgg
    18061 gtgaggtgac cgcggttccc gggcgtccaa tccccgtcgc tcaactggca gacgcggatg
    18121 cgctgatggt gcgttcggtc acgaaagtga atgaatcttt gctggcagga aaacccatta
    18181 aatttgttgg cactgccact gcggggaccg accatgtcga tgaagcatgg ttgaagcagg
    18241 cgggaattgg tttttccgct gcacctggct gtaatgcgat tgcggtggtg gaatatgttt
    18301 tctcctcact actgatgctt gccgaacgcg atggattttc actgcacgac cgtacggtgg
    18361 ggatcgtggg cgttggtaac gttggacgtc gattgcaggc gcgactggaa gcgttaggga
    18421 ttaaaacatt gctttgcgat ccgcctcgcg ctgaccgtgg ggatgagggt gatttccgct
    18481 cgctggatga attagttcag catgcggata ttctgacttt ccatacgcca ctcttcaaag
    18541 atgggccgta caaaacgctg catctggcgg atgaaaaact gatccgtagc ctgaagccgg
    18601 gagcgattct gattaacgcc tgccgtggcg cagtcgtcga taatactgcg ttgctgacct
    18661 gcctgaatga aggccagaag ttaagcgtag tgctggatgt ctgggaaggc gaaccggaac
    18721 ttaacgtgga gttgctgaca aaagtggata tcggcacgcc gcatatcgca ggttataccc
    18781 tggaaggtaa agcacgcggt actacgcaag tgtttgaagc ctatagcaag tttattgggc
    18841 atgaacagca cgttgcgctg gatacattac tgcccgcgcc agagtttggt cgcattacgc
    18901 tgcatggccc gctcgatcaa ccgacgctga aaaggctggt gcatttggtg tatgatgtgc
    18961 gccgcgatga cgcaccgctg cgtaaagtcg ccgggatacc gggtgagttc gataaactgc
    19021 gcaaaaacta tcttgagcgc cgtgaatggt catctctgta tgtaatttgt gatgacgcca
    19081 gtgcggcatc attgctgtgt aaactgggtt ttaacgccgt tcatcatccg gcacgttaat
    19141 ctcttcttca tgctctctgc tgtaacattg gcagggagct ttgctatttc tggagtaaac
    19201 caccatgtct gaaggctgga acattgccgt cctgggcgca actggcgctg tgggcgaagc
    19261 cctgcttgaa acgctggctg aacgtcagtt cccggttggg gaaatttatg cactggcacg
    19321 taacgaaagc gcaggcgaac aactgcgctt tggtggtaaa acaatcactg tgcaggatgc
    19381 cgctgaattc gactggacgc aggcgcagct ggcatttttt gtcgcaggca aagaagctac
    19441 cgctgcctgg gttgaagaag cgaccaactc aggttgcctg gtgatcgaca gcagcggact
    19501 gtttgctctc gaacccgacg taccgctggt ggtgccggaa gtaaacccgt ttgtactgac
    19561 cgattaccgc aaccggaatg tcatcgccgt accagacagt ctgaccagcc agctgctggc
    19621 ggcactgaaa ccgttaatcg atcagggcgg tttgtcgcgt atcagtgtta ccagcctgat
    19681 ttcagcctcc gcccagggca aaaaagcggt cgatgcgtta gcggggcaga gtgcgaaatt
    19741 gctcaacggc attccgattg acgaagaaga tttcttcggt cgtcaactgg cgttcaatat
    19801 gctgccgttg ttgccggata gcgaaggtag cgtgcgtgaa gaacgtcgta tcgttgacga
    19861 agtacgcaaa atcctgcagg acgaagggct gatgatttcg gcaagcgtcg tccaggcacc
    19921 ggtattctac ggtcatgccc agatggtcaa ctttgaagca ctgcgtccgc tggcggcaga
    19981 agaagcgcgt gatgcgtttg ctcagggcga agatattgtg ctctctgaag agaacgaatt
    20041 cccgactcag gtaggggatg cttcgggtac gccgcatctt tctgttggct gcgtgcgtaa
    20101 tgactacggt atgccggagc aagtccagtt ctggtcggtg gccgataacg ttcgctttgg
    20161 cggcgcgctg atggcagtaa aaatcgccga gaaactggtg caggagtatc tgtactaatg
    20221 tccgaccagc aacaactgcc agtttataaa attgcgctgg gcattgagta cgacggcagt
    20281 aagtattacg gctggcaacg gcagaatgaa gtccgcagtg tgcaggagaa gctggaaaag
    20341 gcgctctccc aggtggcgaa cgaacccatc accgtcttct gcgccgggcg tactgacgca
    20401 ggggtacacg gtaccgggca ggtggtgcat ttcgaaacaa ccgcgcagcg caaagacgcg
    20461 gcgtggacct tgggcgtaaa tgcgaattta cctggtgaca tcgccgtgcg ttgggttaaa
    20521 gctgtacctg atgattttca tgcccgattt agcgccacgg ctcgccgtta tcgctacatc
    20581 atctacaatc atcggctgcg cccggcggta ttgagtaaag gggtaaccca tttttacgaa
    20641 ccgctggacg ctgaacggat gcatcgggct gcgcaatgct tgctgggcga gaatgatttc
    20701 acctcgttcc gtgcggtgca gtgccagtcc cgaaccccgt ggcgcaacgt tatgcatatt
    20761 aatgtcacgc gtcacggtcc ttatgtggtg gtagatatca aagcgaatgc ctttgtacat
    20821 catatggtca ggaatattgt cggcagcctg atggaagtcg gtgcccacaa ccagccggag
    20881 agatggatag cagagttgct ggcggcaaag gacagaacgc tggcggcagc aacggcaaaa
    20941 gcggaagggc tgtatctggt cgcggtggat taccctgacc ggtatgatct tccaaaaccg
    21001 ccaatgggcc cgctatttct ggcggactaa cgagaataat gctcggaaca tttcaggctg
    21061 tagccaacgt agttacagcc tgaaagatga cgagtacaag gcataggcaa atatggacct
    21121 gatttatttc ctcatcgatt ttatcctgca cattgacgtg catctggcag agctggtcgc
    21181 ggagtacggc gtctgggttt atgccattct gtttttgatt ctgttctgcg aaacggggct
    21241 ggtggtaacg ccattcctgc ctggtgattc gctgctgttt gttgctggtg cattggcgtc
    21301 gctggaaact aacgatctca atgtccatat gatggtggtg cttatgttga ttgccgctat
    21361 tgtcggtgat gcagtgaact ataccattgg acggttattt ggtgacaaac tgttcagtaa
    21421 cccggattcg aaaatctttc gtcgcagtta tcttgataag actcatcagt tttacgagag
    21481 acatggcggc aaaacgatta ttctcgcccg ttttgtaccg atcgtcagaa cgttcgcgcc
    21541 gtttgttgcg ggaatgggcc acatgtcgta ccgtcatttc gccgcttata acgtgatcgg
    21601 cgcactgttg tgggtactga tttttaccta cgcgggttat ttcttcggca cgctcccctt
    21661 cattcagagc aaccttaagc tgatgattgt tggcattatt tttgtttcaa tattgcctgg
    21721 ggttttcgaa tttattcgtc acaaacgcgc tgcggcacgc gccgcaaaat aaaaagtaac
    21781 tccgcggttc gaccactttt ttatccaaag tttcgggctg ttatgtttta atgtgcaaca
    21841 ttcatggtct gttgggggca aaaatggcat tatgcgtccc caaagataaa actggcatcg
    21901 aaccaggttc agacagaaag gtccctaatg agctggattg aacgaattaa aagcaacatt
    21961 actcccaccc gcaaggcgag cattcctgaa ggggtgtgga ctaagtgtga tagctgcggt
    22021 caggttttat accgcgctga gctggaacgt aatcttgagg tctgtccgaa gtgtgaccat
    22081 cacatgcgta tgacagcgcg taatcgcctg catagcctgt tagatgaagg aagccttgtg
    22141 gagctgggta gcgagcttga gccgaaagat gtgctgaagt ttcgtgactc caagaagtat
    22201 aaagaccgtc tggcttctgc gcagaaagaa accggcgaaa aagatgcgct ggtggtgatg
    22261 aaaggcactc tgtatggaat gccggttgtc gctgcggcat tcgagttcgc ctttatgggc
    22321 ggttcaatgg ggtctgttgt gggtgcacgt ttcgtgcgtg ccgttgagca ggcgctggaa
    22381 gataactgcc cgctgatctg cttctccgcc tctggtggcg cacgtatgca ggaagcactg
    22441 atgtcgctga tgcagatggc gaaaacctct gcggcactgg caaaaatgca ggagcgcggc
    22501 ttgccgtaca tctccgtgct gaccgacccg acgatgggcg gtgtttctgc aagtttcgcc
    22561 atgctgggcg atctcaacat cgctgaaccg aaagcgttaa ttggctttgc cggtccgcgt
    22621 gttatcgaac agaccgttcg cgaaaaactg ccgccaggat tccagcgcag tgaattcctg
    22681 atcgagaaag gcgccatcga catgatcgtc catcgtccgg aaatgcgcct gaaactggcg
    22741 agcattctgg cgaagttgat gaatctgcca gcgccgaatc ctgaagcgcc gcgtgaaggc
    22801 gtagtggtac ccccggtacc ggatcaggaa cctgaggcct gataactgat aagggcaggg
    22861 ccactggctc tgcccttttg ctattctcac cgtaacgaat cagcggatac catgattatc
    22921 aaacgcactc ctcaagccgc gtcgcctctg gcttcgtggc tttcttatct ggaaaacctg
    22981 cacagtaaaa ctatcgatct cggccttgag cgcgtgagcc aggtcgcggc gcgtcttggc
    23041 gtcctgaaac cagcgccatt tgtgtttacc gttgcgggta cgaatggcaa aggcaccacc
    23101 tgccgtacgc tggagtcgat tctgatggcg gcagggtaca aagtgggcgt ctacagttca
    23161 ccgcatctgg tgcgttatac cgagcgcgta cgtgtgcagg ggcaggaatt gccggaatcg
    23221 gcccacaccg cctcttttgc ggagattgaa tcggcacgcg gtgatatttc cctgacctat
    23281 ttcgagtacg gtacgctgtc ggcgttgtgg ctgttcaaac aggcacaact tgacgtagtg
    23341 attctggaag tagggctggg cggtcgtctg gacgcaacca atattgtcga tgccgatgtg
    23401 gctgtagtaa ccagcattgc gctggatcat accgactggc tgggaccaga tcgcgaaagt
    23461 attggtcgcg agaaagcagg catctttcgc agcgcaaaac cggcaattgt cggtgagccg
    23521 gaaatgcctt ctaccattgc tgatgtggcg caggaaaaag gtgcactgtt acaacgtcgg
    23581 ggcgttgagt ggaactattc cgtcaccgat catgactggg cgtttagcga tgctcacggc
    23641 acgctggaaa atctgccgtt gccgcttgtc ccgcaaccga atgccgcaac agcgctggcg
    23701 gcactgcgtg ccagcgggct ggaagtcagt gaaaatgcca ttcgcgacgg gattgccagc
    23761 gcaattttgc cgggacgttt ccagattgtg agcgagtcgc cacgcgttat ttttgatgtc
    23821 gcgcataatc cacatgcggc ggaatatctc accgggcgta tgaaagcgct accgaaaaac
    23881 gggcgcgtgc tggcggttat cggtatgcta catgataaag atattgccgg aactctggcc
    23941 tggttgaaaa gcgtggttga tgactggtat tgtgcgccac tggaagggcc gcgcggtgcc
    24001 acggcagaac aactgcttga gcatttgggt aacggcaaat catttgatag cgttgcgcag
    24061 gcatgggatg ccgcaatggc ggacgctaaa gcggaagaca ccgtgctggt gtgtggttct
    24121 ttccacacgg tcgcacatgt catggaagtg attgacgcga ggagaagcgg tggcaagtaa
    24181 gtttcagaat cggttagtgg gcacgatcgt gctggtggcg ctgggggtga ttgtacttcc
    24241 agggctgctg gacgggcaga aaaaacatta tcaggatgag tttgcggcta tcccgctggt
    24301 tccaaaagcg ggcgatcgcg atgagcctga tatgatgcca gccgccaccc aggcgttgcc
    24361 gacgcagccg ccggaaggcg cagcggaaga ggtgcgggca ggtgatgccg cagcaccgtc
    24421 gctcgatccg gccactattg cagccaataa caccgagttt gaaccggaac ctgcaccggt
    24481 cgccccaccg aagccgaaac cggtggagcc gcctaaaccg aaggttgaag caccacctgc
    24541 gccgaagcca gaaccgaagc cggtcgtgga agaaaaagca gcgccaacgg gtaaagccta
    24601 tgttgtgcaa ctgggtgcgc tgaaaaatgc cgataaagtg aatgagattg tcggtaagct
    24661 gcgcggtgcc ggttatcggg tttatacgtc gccatccacg ccagtgcagg gtaaaattac
    24721 ccgtattctg gttgggccgg atgcctcgaa agataagctg aaaggttcgc tgggtgagtt
    24781 gaagcaactt tctggcttaa gtggcgtggt aatgggctat acgccgaatt aatacagtct
    24841 tgcctgatgc gacgctggcg cgtcttatca ggcctgggca gtgttagaac cgtaggtcgg
    24901 ataaggcgtt tacgccgcat ccgacacgca ttgcacgatg ccgcaaaggc ataaaaagtc
    24961 gatggcgttg aatatttttt cagcgccatt tttattgatg cgtgggaagg aaatccctac
    25021 gcaaacgttt tctttttctg ttagaatgcg ccccgaacag gatgacaggg cgtaaaatcg
    25081 tgggacacat atggtctgga ttgattacgc cataatcgcg gtgattgctt tttcctctct
    25141 ggttagcctg atccgcggct ttgttcgtga agcgttatcg ctggtgacat ggggttgtgc
    25201 tttctttgtt gccagtcatt actacactta cctgtcagtc tggtttacgg gctttgaaga
    25261 cgaactggtt cgaaatggaa ttgccatcgc ggtactgttt atcgctaccc tgattgttgg
    25321 tgctatcgtg aacttcgtga taggccagtt ggtggagaaa acggggttgt caggcaccga
    25381 tcgggtgctg ggcgtctgtt tcggtgcgtt gcgcggtgtg ttgattgttg ccgccattct
    25441 cttctttctc gactccttta ccggggtgtc gaaaagcgaa gactggagca aatcacagct
    25501 gatcccgcag ttcagtttta tcatcagatg gttttttgat tatctgcaaa gctcgtcaag
    25561 tttcttgccc agagcgtaag tgctctgaga tgtggcttaa cgaggaaaaa gacgtatgtg
    25621 cggtattgtc ggtatcgccg gtgttatgcc ggttaaccag tcgatttatg atgccttaac
    25681 ggtgcttcag catcgcggtc aggatgccgc cggcatcatc accatagatg ccaataactg
    25741 cttccgtttg cgtaaagcga acgggctggt gagcgatgta tttgaagctc gccatatgca
    25801 gcgtttgcag ggcaatatgg gcattggtca tgtgcgttac cctacggctg gcagctccag
    25861 cgcctctgaa gcgcagccgt tttacgttaa ctccccgtat ggcattacgc ttgcccacaa
    25921 cggcaatctg accaacgctc acgagttgcg taaaaaactg tttgaagaaa aacgccgcca
    25981 catcaacacc acttccgact cggaaattct gcttaatatc ttcgctagcg agctggacaa
    26041 cttccgccac tacccgctgg aagccgacaa tattttcgct gccattgctg ccacaaaccg
    26101 cttaatccgc ggcgcatatg cctgtgtggc gatgattatc ggccacggta tggttgcttt
    26161 ccgcgatcca aacgggattc gtccgctggt actgggaaaa cgtgatattg acgagaaccg
    26221 tacagaatat atggtcgctt ccgaaagcgt agcgctcgat acgctgggct ttgatttcct
    26281 gcgtgacgtc gcgccgggcg aagcgattta catcactgaa gaagggcagt tgtttacccg
    26341 tcaatgtgct gacaatccgg tcagcaatcc gtgcctgttt gagtatgtat actttgctcg
    26401 cccggactcg ttcatcgaca aaatttccgt ttacagcgcg cgtgtgaata tgggcacgaa
    26461 actgggcgag aaaattgccc gcgaatggga agatctggat atcgacgtgg tgatcccgat
    26521 cccagaaacc tcgtgtgata tcgcgctgga aattgctcgt attctgggca aaccgtaccg
    26581 ccagggcttc gttaaaaacc gctatgttgg ccgcaccttt atcatgccgg gccagcagct
    26641 gcgtcgtaag tccgtgcgcc gtaaactgaa tgccaaccgc gccgagttcc gcgataaaaa
    26701 cgtcctgctg gtcgacgact ccatcgtccg tggcaccact tctgagcaga ttatcgagat
    26761 ggcacgcgaa gccggagcga agaaagtgta cctcgcttct gcggcaccgg aaattcgctt
    26821 cccgaacgtt tatggtattg atatgccgag cgccacggaa ctgatcgctc acggtcgcga
    26881 agttgatgaa attcgccaga tcatcggtgc tgacgggttg attttccagg atctgaacga
    26941 tctgatcgac gccgttcgcg ctgaaaatcc ggatatccag cagtttgaat gctcggtgtt
    27001 caacggcgtc tacgtcacca aagatgttga tcagggctac ctcgatttcc tcgatacgtt
    27061 acgtaatgat gacgccaaag cagtgcaacg tcagaacgaa gtggaaaatc tcgaaatgca
    27121 taacgaagga tgatgccttc gctgagggtg ccggtctggc accctgactt gcaactcccg
    27181 ccgaaatact gcaaagtctg cctgcaagtc tgacagggca actatttatg aaacgactca
    27241 ttgtaggcat cagcggtgcc agcggcgcga tttatggcgt gcgcttatta caggttctgc
    27301 gcgatgtcac agatatcgaa acgcatctgg tgatgagcca ggcggcgcgc cagaccttat
    27361 ccctcgaaac ggatttttcc ctgcgcgaag tgcaggcatt agctgatgtc acgcacgatg
    27421 cgcgcgatat ttccgccagc atctcttccg gttctttcca gacgctgggg atggtgattt
    27481 taccctgttc aatcaaaacc ctttccggca ttgtccatag ctatactgat ggcttactga
    27541 cccgtgcggc agatgtggtg ctgaaagagc gtcgcccgtt ggtgctctgc gtgcgtgaaa
    27601 caccattgca cttaggccat ctgcgtttaa tgactcaggc ggcagaaatc ggtgcggtga
    27661 ttatgcctcc cgttccggcg ttttatcatc gcccgcaatc ccttgatgat gtgataaatc
    27721 agacggttaa tcgtgttctt gaccagtttg caataaccct tcctgaagat ctctttgccc
    27781 gctggcaggg cgcataactc cgctgttgcc ctgtttcagg gcaattttgc aaccgcgatc
    27841 aaatcctcga cattttgttt ctgccattca atcgaaacgc tgcgattcaa ccgctatacc
    27901 tgctatcttc aacttcagga caataatgca acgtcttatt aacatattta acgttgaatg
    27961 ttactgttgt cgtcaagatg gcataagacc tgcatgaaag agcctgcaaa cacacaacac
    28021 aatacacaac ataaaaaagc cattttcact tgagggttat gtatgaagaa gtcgattctc
    28081 gctctgtctt tgttagtcgg tctctccgca gcggcttcca gctatgcggc gctaccggag
    28141 acggtacgta tcggaaccga taccacctat gcgccgttct cgtcgaaaga tgctaaaggt
    28201 gattttgttg gctttgatat cgatctcggt aacgagatgt gcaaacggat gcaggtgaaa
    28261 tgtacctggg ttgccagtga ctttgacgcg ctgatcccct cactgaaagc gaaaaaaatc
    28321 gacgccatta tttcgtcgct ttccattacc gataaacgtc agcaggagat tgccttctcc
    28381 gacaagctgt acgccgcaga ttctcgtttg attgctgcca aaggttcacc gattcagcca
    28441 acgctggatt cactgaaagg taaacatgtt ggtgtgctgc agggatcaac ccaggaagct
    28501 tacgctaacg agacctggcg tagcaaaggc gtggatgtgg tggcctatgc caaccaggat
    28561 ttggtctatt ccgatctggc tgcaggacgt ctggatgctg cgttacaaga tgaagttgct
    28621 gccagcgaag gattcctcaa gcaacctgct ggtaaagctt tcgcctttgc tggctcatca
    28681 gtaaaagaca aaaaatactt cggtgatggc accggtgtag ggctacgtaa agatgatgct
    28741 gaactgacgg ctgccttcaa taaggcgctt ggcgagctgc gtcaggacgg cacctacgac
    28801 aagatggcga aaaagtattt cgactttaat gtctacggtg actgatacgt cgctgggaag
    28861 ctgtacctga tggaatgatc atcatggtgc acgccaggtt tgttgcacta tcgtggtgca
    28921 ttgaaatgca tacttaagca tttttaatga aaaataatac gtctaacggg gcggaatatt
    28981 ttgccttgat ggtcaatttt atggcacgat aagtgtaaca aacctgtaaa tattccctat
    29041 aaaaagactg tcagttgagg acattatgaa aaaactggtg ctatcgctct ctctggttct
    29101 ggccttctcc agcgcaactg cggcatttgc tgcgattccg caaaacatcc gcatcggtac
    29161 cgacccgacc tatgcgccat ttgaatcaaa aaattcacaa ggcgaactgg ttggcttcga
    29221 tatcgatctg gcaaaggaat tatgcaaacg catcaatacg caatgtacgt ttgtcgaaaa
    29281 tccgctggat gcgttaatcc cgtccttaaa agcgaagaag attgacgcca tcatgtcatc
    29341 gctttccatt acggaaaaac gtcagcaaga aatagccttc accgacaaac tgtacgctgc
    29401 cgattctcgt ttggtggtgg cgaaaaattc tgacattcag ccgacagtcg agtcgctgaa
    29461 aggcaaacgg gtaggcgtat tgcagggcac cacccaggag acgttcggta atgaacattg
    29521 ggcaccaaaa ggcattgaaa tcgtctcgta tcaggggcag gacaacattt attctgacct
    29581 gactgccgga cgtattgatg ccgcgttcca ggatgaggtc gctgccagcg aaggtttcct
    29641 caaacaacct gtcggtaaag attacaaatt cggtggcccg tctgttaaag atgaaaaact
    29701 gtttggcgta gggaccggca tgggcctgcg taaagaagat aacgaactgc gcgaagcact
    29761 gaacaaagcc tttgccgaaa tgcgcgctga cggtacttac gagaaattag cgaaaaagta
    29821 cttcgatttt gatgtttatg gtggctaatc gccatcggtg aaagaaagcc cgctcccttc
    29881 gggtaaccgg agggagacaa gaggcggtaa ttcaccacac acgacaggac aggcagcatg
    29941 ttgtatgggt tttcaggtgt tattttacag ggtgcgctcg tcacgctgga gctggctatc
    30001 agctctgtag tgctcgctgt aatcatcggt ttaattggcg ctggcggtaa gctctcgcaa
    30061 aatcggcttt cggggctgat tttcgaaggg tacaccacgc tgattcgtgg cgtgccggac
    30121 ttagtgttga tgctgctgat tttctacggt ttgcagattg cgctaaacac ggtgacggag
    30181 gcgatgggcg tcgggcagat tgatatcgat ccgatggtcg ctggtattat cactctcggt
    30241 tttatctacg gtgcttattt taccgaaacg ttccgtggtg cttttatggc agtgccgaaa
    30301 ggacatatag aggcggcgac ggcgttcggt tttactcgtg ggcaagtgtt tcggcggatc
    30361 atgtttccgg cgatgatgcg ttacgccctg ccaggcattg gcaacaactg gcaggtgatc
    30421 ctcaaatcta ccgcactggt ttcgttactc ggcctggaag atgtggtcaa agccacccaa
    30481 ctggcaggca aaagtacctg ggaaccgttc tatttcgcca tcgtctgtgg cgtgatttac
    30541 ctggttttca ccactgtttc caatggtgtg ctgctgttcc ttgagcgccg ctactccgtg
    30601 ggtgtgaaga gggctgacct gtgatcgaaa tcttacatga atactggaaa ccgctgctgt
    30661 ggaccgacgg ttatcgcttt actggtgtgg cgatcactct gtggctgctt attttgtcgg
    30721 tagtgatagg cggagtcctg gcgctgtttc tggcgattgg tcgtgtctcc agtaataaat
    30781 acatccagtt tccaatctgg ttatttacct atatttttcg cggtacgccg ctgtatgttc
    30841 agttgctggt gttctattcc ggcatgtaca cgcttgagat tgttaaggga accgaattcc
    30901 ttaacgcttt cttccgcagt ggcctgaact gtaccgtgct ggcgctgacg cttaacacct
    30961 gcgcttacac taccgagatt tttgctgggg caatccgttc ggttccgcat ggggaaattg
    31021 aagccgccag agcctatggc ttctcgactt ttaaaatgta tcgttgcatt attttgcctt
    31081 ctgcgctgcg tattgcgtta ccggcataca gcaacgaagt gatcctgatg ctgcactcta
    31141 ctgcgctggc gtttacggcc actgtgccgg atctgctgaa aatagcccgc gatattaacg
    31201 ccgccacgta tcaacctttt accgccttcg gcattgccgc ggtgctctat ttaatcatct
    31261 cttatgtcct gatcagcctc tttcgcagag cggaaaaacg ctggttgcag catgtgaaac
    31321 cttcttcaac gcactgagaa tacgatgtcc gagaataaat taaacgttat cgatttgcac
    31381 aaacgctacg gcgaacatga agtgctgaaa ggggtctcac tgcaagcgaa tgccggagat
    31441 gtaataagca tcatcggatc gtcgggatcg gggaaaagta cctttctgcg ctgcattaac
    31501 ttcctcgaaa aaccgagtga agggtcgatc gtggtcaatg gccagacgat caatctggtg
    31561 cgcgacaaag acggtcaact caaagtcgcc gataaaaatc aactgcgctt actgcgcaca
    31621 cgcctgacga tggtattcca gcacttcaat ctctggagcc atatgacggt gctggaaaac
    31681 gtcatggaag cgccgattca ggtgttgggc ctgagcaagc aggaagcgcg cgagcgggcg
    31741 gtgaagtatc tggcgaaagt cgggatagac gaacgtgcgc aggggaaata tccggtgcat
    31801 ctttccggcg gtcagcaaca gcgtgtttct atcgcgcggg cgctggcgat ggaaccggaa
    31861 gttttactgt ttgatgaacc tacctcggcg ctcgatcctg aactggtagg cgaagtgtta
    31921 cgtattatgc agcaactggc agaagagggg aaaaccatgg tggtagtgac tcacgaaatg
    31981 ggctttgctc gccatgtttc tactcacgtc attttcctcc atcaggggaa aatagaagaa
    32041 gagggcgcgc cggagcagtt atttggcaac ccgcaaagcc ctcgtctgca acagttcctt
    32101 aagggatcgc tgaaataatt tcctctccgg tggctaacgc tgccggagta ttttcatggg
    32161 gaatttgcga gtcagcttcc gctgaaatgt aaaagttgca gtccgctaaa aatacttctg
    32221 aattcgctgt ccaaagataa aaactgcctg ctgcattggg tgttaatcgt ggttattgtc
    32281 gcgtcatctt aaatttgagg ggtgacggaa cgccatgaca atatcgacaa cttccacgcc
    32341 gcatgatgcg gtatttaaat cttttttacg ccatccag
//
LOCUS       DAZSBF010000025        31698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZSBF010000025 DAZSBF010000000
VERSION     DAZSBF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.025"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1132
                     /gene="ypdA"
                     /locus_tag="ACHXWD_000870"
     CDS             <1..1132
                     /gene="ypdA"
                     /locus_tag="ACHXWD_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR7619864.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1147..1881
                     /gene="ypdB"
                     /locus_tag="ACHXWD_000871"
     CDS             1147..1881
                     /gene="ypdB"
                     /locus_tag="ACHXWD_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR7619865.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1894..2751
                     /locus_tag="ACHXWD_000872"
     CDS             1894..2751
                     /locus_tag="ACHXWD_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7619866.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="ACHXWD_000873"
                     /pseudo
     CDS             complement(2754..5249)
                     /gene="ptsP"
                     /locus_tag="ACHXWD_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="ACHXWD_000874"
     CDS             complement(5274..6311)
                     /gene="ypdE"
                     /locus_tag="ACHXWD_000874"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR7619867.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="ACHXWD_000875"
                     /pseudo
     CDS             complement(6311..7396)
                     /gene="ypdF"
                     /locus_tag="ACHXWD_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7411..8658)
                     /locus_tag="ACHXWD_000876"
     CDS             complement(7411..8658)
                     /locus_tag="ACHXWD_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFR7619868.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8680..9006)
                     /locus_tag="ACHXWD_000877"
     CDS             complement(8680..9006)
                     /locus_tag="ACHXWD_000877"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR7619869.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9226..10191)
                     /gene="glk"
                     /locus_tag="ACHXWD_000878"
     CDS             complement(9226..10191)
                     /gene="glk"
                     /locus_tag="ACHXWD_000878"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR7619870.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10395..11651
                     /locus_tag="ACHXWD_000879"
     CDS             10395..11651
                     /locus_tag="ACHXWD_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR7619871.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11766..12092
                     /gene="ypeC"
                     /locus_tag="ACHXWD_000880"
     CDS             11766..12092
                     /gene="ypeC"
                     /locus_tag="ACHXWD_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR7619872.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12139..13377)
                     /locus_tag="ACHXWD_000881"
     CDS             complement(12139..13377)
                     /locus_tag="ACHXWD_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR7619873.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13713..14915
                     /gene="nupC"
                     /locus_tag="ACHXWD_000882"
     CDS             13713..14915
                     /gene="nupC"
                     /locus_tag="ACHXWD_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR7619874.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(14965..17153)
                     /locus_tag="ACHXWD_000883"
                     /pseudo
     CDS             complement(14965..17153)
                     /locus_tag="ACHXWD_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(17362..17437)
                     /locus_tag="ACHXWD_000884"
     tRNA            complement(17362..17437)
                     /locus_tag="ACHXWD_000884"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17402..17404),aa:Ala,seq:ggc)
     gene            complement(17477..17552)
                     /locus_tag="ACHXWD_000885"
     tRNA            complement(17477..17552)
                     /locus_tag="ACHXWD_000885"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17517..17519),aa:Ala,seq:ggc)
     gene            17773..18132
                     /locus_tag="ACHXWD_000886"
     CDS             17773..18132
                     /locus_tag="ACHXWD_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR7619875.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18134..18526
                     /locus_tag="ACHXWD_000887"
     CDS             18134..18526
                     /locus_tag="ACHXWD_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR7619876.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18578..19993)
                     /gene="gltX"
                     /locus_tag="ACHXWD_000888"
     CDS             complement(18578..19993)
                     /gene="gltX"
                     /locus_tag="ACHXWD_000888"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR7619877.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20252..20327
                     /locus_tag="ACHXWD_000889"
     tRNA            20252..20327
                     /locus_tag="ACHXWD_000889"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20285..20287,aa:Val,seq:tac)
     gene            20372..20447
                     /locus_tag="ACHXWD_000890"
     tRNA            20372..20447
                     /locus_tag="ACHXWD_000890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20405..20407,aa:Val,seq:tac)
     gene            20494..20569
                     /locus_tag="ACHXWD_000891"
     tRNA            20494..20569
                     /locus_tag="ACHXWD_000891"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20527..20529,aa:Val,seq:tac)
     gene            20574..20649
                     /locus_tag="ACHXWD_000892"
     tRNA            20574..20649
                     /locus_tag="ACHXWD_000892"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20607..20609,aa:Lys,seq:ttt)
     gene            20680..20805
                     /locus_tag="ACHXWD_000893"
     ncRNA           20680..20805
                     /ncRNA_class="other"
                     /locus_tag="ACHXWD_000893"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            20925..21266
                     /locus_tag="ACHXWD_000894"
     CDS             20925..21266
                     /locus_tag="ACHXWD_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR7619878.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21257..22183)
                     /locus_tag="ACHXWD_000895"
     CDS             complement(21257..22183)
                     /locus_tag="ACHXWD_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7619879.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22273..23271
                     /locus_tag="ACHXWD_000896"
     CDS             22273..23271
                     /locus_tag="ACHXWD_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR7619880.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23268..23486)
                     /locus_tag="ACHXWD_000897"
     CDS             complement(23268..23486)
                     /locus_tag="ACHXWD_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR7619881.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23488..25503)
                     /gene="ligA"
                     /locus_tag="ACHXWD_000898"
     CDS             complement(23488..25503)
                     /gene="ligA"
                     /locus_tag="ACHXWD_000898"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR7619882.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25574..26560)
                     /gene="zipA"
                     /locus_tag="ACHXWD_000899"
     CDS             complement(25574..26560)
                     /gene="zipA"
                     /locus_tag="ACHXWD_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR7619883.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26790..27551
                     /gene="cysZ"
                     /locus_tag="ACHXWD_000900"
     CDS             26790..27551
                     /gene="cysZ"
                     /locus_tag="ACHXWD_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR7619884.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27736..28707
                     /gene="cysK"
                     /locus_tag="ACHXWD_000901"
     CDS             27736..28707
                     /gene="cysK"
                     /locus_tag="ACHXWD_000901"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR7619885.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29091..29348
                     /gene="ptsH"
                     /locus_tag="ACHXWD_000902"
     CDS             29091..29348
                     /gene="ptsH"
                     /locus_tag="ACHXWD_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR7619886.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29393..31120
                     /gene="ptsI"
                     /locus_tag="ACHXWD_000903"
     CDS             29393..31120
                     /gene="ptsI"
                     /locus_tag="ACHXWD_000903"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR7619887.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31161..31670
                     /gene="crr"
                     /locus_tag="ACHXWD_000904"
     CDS             31161..31670
                     /gene="crr"
                     /locus_tag="ACHXWD_000904"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR7619888.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7821 a         8355 c         7880 g         7642 t
ORIGIN      
        1 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcaga ccaagctggc
       61 gctggatatc gccaacaaaa cgctaccgct gtttcgccat gtcaatagcg agtcattacg
      121 caaggtctgc gaaattatcc gcgatgacat tcacgccgat gcggtggcga ttactaatac
      181 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
      241 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
      301 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
      361 ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
      421 acaggaaatg gcggtcggtc tgtcgcaaat catctccacg caactggagg tttcacgcgc
      481 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
      541 tccccatttt ctgtttaacg ctctgaacgc tatttcatcg tcaatccgtc tgaatccgga
      601 taccgctcgc cagttgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
      661 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
      721 tgagcaggcc cgctttggtg acaagctgac ggttatctat gatattgatg aagaggtgaa
      781 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aacgccattg tccacggtat
      841 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gaaatcgggt
      901 acgcattgcg gtgcgagata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
      961 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gtccatcatc gcgtgaagtt
     1021 attgtatggc gaggggctgc atatccgccg cctggagccg gggacggaaa ttgcgtttta
     1081 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     1141 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     1201 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     1261 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     1321 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     1381 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     1441 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     1501 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     1561 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     1621 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     1681 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     1741 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     1801 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     1861 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     1921 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     1981 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     2041 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     2101 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     2161 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     2221 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     2281 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     2341 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgcaa cgtggtgaat
     2401 atgccgccgg gtaattcgca accgcgcggc gattttctct atcacagcat ttgtaactgg
     2461 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     2521 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     2581 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     2641 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     2701 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     2761 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     2821 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     2881 atcccttcgt tagcacccag cgtcagcatg atcaccagtt caacttcgcc catttctgac
     2941 tgccagtcaa ccggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     3001 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     3061 tgccagacat cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     3121 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     3181 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     3241 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     3301 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     3361 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     3421 atgcctaccc atttgccccg ctgatgcgca gtggtaacta tttgctgcaa catgcgcagg
     3481 aacgatggcg taatcgggtt atatagcggc gatacgcgcg ggttattacg atcgaccgca
     3541 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     3601 tggtcgatga tgtagcacac cgacggcact tccaccatga tcccaagcgt aatcgtctct
     3661 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gcctttcacc
     3721 cataagatct gatcgaggct gttaaccatc gggatcatca actgggcgtt gccgaaactg
     3781 gcggcgcgca aaatggcccg cagttgagtg cggaacaggc cagcaaattc cgggtaaata
     3841 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     3901 cttttatcgc cgccgatgtc catcgtgcgg aagataatcg gcttgtcgcc cgccgccagt
     3961 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     4021 tagagcattt cggtacggaa cagcccgaca ccttccgcgc cgttggcaaa cgcgcctggc
     4081 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     4141 gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgcgcg
     4201 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     4261 tcaagcactg caggttgccc ggcataacgg gcaatcgcat ccagcggcaa gccactcagt
     4321 accgggatcg ccgaggcacg ggccagaatc agtgtatgcg aggtgcgccc ggttttctcc
     4381 agaatcatgc ccgcaagatt tttcaaatca aggctcaaaa actggcttgg ggttaaatct
     4441 tcagccacca gaatggtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     4501 taggtgatat gcagcaactg ttcgctgata tcgcgaatgt cgctgacccg ctcgcgcaga
     4561 taatcgctgg cagaagcaga tagtttggcg caaacctgct ccatattgct gatgatcgcc
     4621 gcccccagtc cctgatgctg ttctgtcatc aggcgacgga tattgcctgc aaattcatca
     4681 tcctgaatca gcgacaaatg agcgctgagg atagttttgc tttcgccgtc acgctcacgc
     4741 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     4801 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     4861 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     4921 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     4981 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcgcctg ctcttcatcg
     5041 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     5101 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgataaaggt gatttcgctt
     5161 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     5221 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     5281 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     5341 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     5401 gtggcaggcc ccattaccac tgtgggtacg ccagtatcgg ttaagtgcac cgctccaccg
     5461 tccgtgccgc cgttactgaa catatccgcc tgcaacggaa cgccaatttc tgccgacacg
     5521 gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg atttatcgct taacaccagc
     5581 atcggcccgt taccaatctg acgatggtta gccgcgccat aatcaaagtt tttcgcccag
     5641 caggcggtat caagcacaat ggcgacgtcc ggcgacaccg cgcgggtagc agtttgcccg
     5701 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacttccgc aggcagttcg
     5761 gcgtcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg gtcatcaaag
     5821 gctttcccca tcacccgctg gtgagggaga acctgaaaag ctgtatcaaa ggtgacgcga
     5881 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
     5941 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
     6001 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
     6061 accggcagca catcaattgc cccttcgcgg gagatgctgc gcaccataaa tcccacttcg
     6121 tccatatgcg cacagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
     6181 gatcccagac catcaaatcg tacttctttt tgcaggcgat ccgcttcttc cagtaggatc
     6241 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
     6301 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
     6361 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
     6421 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtatcccgcg
     6481 gtgaaaagcg tggatcttcg tgtacttcaa taccgatagc atgaccggtg ttgtgaccaa
     6541 aatagtcgcc aaatcctgcc tcggtaatca cccggcgcgc ggcgtcgtca acctgttagc
     6601 agcgtacgcc agggcgaatc gcggagattg ccgccagctg tgcttgcagg acaatctgat
     6661 agacgttaaa cagcgggtga gattcggcaa ttaccccttc gccattcacc agcaaggtgc
     6721 gcgtcatatc agagcagtac ccctgataga gcgcaccaaa atcaagagtg acaaactcgc
     6781 ctgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccacgc cagccgctgg
     6841 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
     6901 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
     6961 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
     7021 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
     7081 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
     7141 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
     7201 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
     7261 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
     7321 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
     7381 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttaccttt
     7441 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
     7501 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
     7561 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tcgcgccgac
     7621 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
     7681 aatccccata aaggcaagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
     7741 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agacccagac ctatcggcgg
     7801 aataacgata gcgatagaac gtgcagtgac cggtaatacg tggtcagtgg taaagctgaa
     7861 ggcaacaaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgcgg tcgccgcggc
     7921 gatccccatt gcgtacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
     7981 accgccgttg atccagccgc caaagggggt aatgacgtag tacatcgcca gcatgacaaa
     8041 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
     8101 ctggattttc tggttcatcc acttcacaag gtaacccgcg acgatagaaa taatcagcgc
     8161 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
     8221 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
     8281 cagtttgcca ccgatagagt tcgccacaaa agcagcaaac atcggaatgg caaaaccaaa
     8341 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
     8401 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
     8461 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
     8521 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
     8581 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
     8641 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
     8701 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
     8761 ttcataaacg tcgcgtgatt cgaaacgctc attatcttcc ggggtaactg ccacggagtg
     8821 gatgatgatg gttgcttcgg cgatatcctg cgccgtcagg cgattctgga taccgtccgc
     8881 gccctgggtt tcaattttca cttcataacc tgcttctacc gccgcttctt ccagcgcctg
     8941 agcggccata aaggtgtgag ccaggcccat tgggcaggca cataaggcaa ttagtttctt
     9001 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
     9061 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
     9121 tgtgacgcag gtcggccttg tgagggggac acaaagaaaa attgaattaa ctgtatgatt
     9181 taaaagatta tcgggagagt tacctcccga tataacagaa aggatttaca gaatgtgccc
     9241 taaggtctgg cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag
     9301 atacaccgga atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg
     9361 gaaaccagag gctttgaaga actcaaggaa gcgcggcacg ataccgccag caatataaac
     9421 cccaccaaag gttcccagcg ttaatgccag attgccgcca aaacggccca taatgacgca
     9481 aaacagcgac aacgcacggc ggcaatcggt gcagctgtca gccagcgcgc gttcggtaat
     9541 atcttttggc ttgagatttt ctggcaggcg gttgtcggct ttaacaattg cgcgatacaa
     9601 attcaccagc ccaggaccag aaagcacgcg ttccgccgaa acatgaccaa tttccgcacg
     9661 caatatttcg aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc
     9721 ttcgcccggc aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc
     9781 tgttccggca ccgtaaaccg caataggttt accttcgacc ggttctgcgc caccaaactg
     9841 aatcagatgc tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat
     9901 aatttccaga tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt
     9961 atggttggtc attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc
    10021 tttcacctcg accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta
    10081 atcaagccct gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc
    10141 aagacgtgcg ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact
    10201 gctccgctaa agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca
    10261 acgccggaaa ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt
    10321 tcagcaccaa ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc
    10381 ataaggaata gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg
    10441 ccgtggcaat tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg
    10501 tattacagaa tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac
    10561 ctatctggat catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt
    10621 tcagccaggg tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc
    10681 cgccctctgc gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca
    10741 gcctggggcc ggaacatccg atcatgaccg tcaatatcgc ccttgccgtc gccattggcg
    10801 ctcgtctgtt accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca
    10861 tcggcgcgct gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg
    10921 gcagtagtga agttccgcta tgggatcgtc tttttgcgcc gttaatggcg gcagcagctg
    10981 gtgcacttac taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg
    11041 gacagatgga aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag
    11101 cagggatggt cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa
    11161 atccggtgct cgtgctgggt attggcggat ttattctcgg aattctgggg gttattggtg
    11221 gaccagtttc gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt
    11281 tcagcaccag cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg
    11341 ccgccagtgg ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag
    11401 ggttgatgct gcatgagcac gttcccgccg ttccagcggc aataaccgtt tcctgcgcta
    11461 ttctcggcat cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcag
    11521 tcgttgtacc caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc
    11581 tgttattagc aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg
    11641 ataatgttta gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg
    11701 ctttacctcc ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa
    11761 taacaatgtt caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag
    11821 caaatgcagg tgaaatcacc ctactgccat cgatcaaatt acaaattggc gatcgcgatc
    11881 attacggtaa ttactgggac ggtggtcact ggcgagatcg tgattactgg catcgcaatt
    11941 atgaatggcg caaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata
    12001 agcgtaaagc atatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg
    12061 gaaaaggccg cggacatggg caccgccatt aaacgcgtcc tccaatggag cacaatgcct
    12121 gatgcgacgc tcattcaact acaatcccag cgccgtcccc accaacaacc agatattcag
    12181 cgccacgacc agcactacaa tcacccagcc tgtctgtttt acgcgtttgc tgttcaccag
    12241 atcgcccatc aacttgctgt cactggtgaa aatcagcagt ggaaccagcg ccagggcgat
    12301 accaaaactt aacagcacct gactcataac cagaatccgt gtcggatcta atcccatcag
    12361 aatgacaata aatgacggca acatggtgac tgtacgacgc acccacagcg ggatatggaa
    12421 gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc cccaccaccg ttgaggacag
    12481 tccggcagca accagactta acccaaagac cgttgccgca gcatggctta acagcggttg
    12541 cagcgtcaga taagcctcat caagatcggc aacaccagta tgaccagaaa agtggaacgc
    12601 cgccgcagct gtagccatca tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc
    12661 cacatcccat ttggtggcgg aataacgttg ttgacgcgaa ccgccatgta aatgctgagt
    12721 gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc gcccctaaca cgcctgctgc
    12781 caggaagacc gcctccgaag taggtaaact cgggatcacc attcctttac ccagctgcgc
    12841 caggttaggc tgggagaaaa tcaactcgac aatgtaagcc gcggcaacaa acaacagtaa
    12901 cccgccaatc actttctcca gtggtttttg cccgcgacgt tgcagcatta aaatcaggaa
    12961 agtcgcgatc cccgtcagca ccgcgccctg caacaacgag acaccaagaa tgagtttaaa
    13021 accgatcgcc gcaccaataa attccgccag gtcggttgcc atcgcaataa tttctgcctg
    13081 aacccaatag aaccacacta cgggacgcgg atagtgatca cgaatctgct ccgccagatt
    13141 ttttccggtg gcaatcccta gtttggcaga gaggatctga atcagcatcg ccatcaggtt
    13201 ggcccaaacg acaacccaca gtagctgata gccgaagctg gcacccgcct gaatattggt
    13261 cgcaaagtta ccgggatcga tataaccaat cgccgcaatg aacgcaggtc ccattaatgc
    13321 gagcctcatc ttgcgcgccg cccgtccgct gctactctca acgcgatagt tcgtcatctt
    13381 gtgcctctaa aacatagcct ttgctatgtt tcatgctatg ataaacgaga atgattatca
    13441 aattcattta aatggattgt ggtgatttct ctgatagacc aggattatga ctacgaaaag
    13501 attgatgact tgaatgttat ggttatgttt aatgttagca catttacata actttcagct
    13561 tccatacaca acatagcaga aatgtatgac agatcactat ttttgaagcc tgtcacagga
    13621 cgtcattata gtgtgtgtca gatctcgttt tccttaacca tgttacatag aatgtgcacg
    13681 gaaatttaac ctgcctcata tttggagcaa atatggaccg cgtccttcat tttgtactgg
    13741 cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta
    13801 tccgttatgt tattcaactg cttgttatcg aagtgttact ggcgtggttc ttcctgaact
    13861 ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat gttcgaaaaa ctgctcagat
    13921 ttgccaacga agggactaac ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct
    13981 tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc actgatcggt attctccagc
    14041 acattcgcgt gttgccggtg attatccgcg caattggttt cctgctctcc aaagtcaacg
    14101 gtatgggcaa actggaatcc tttaacgccg tcagctccct gattctgggt cagtctgaaa
    14161 actttattgc ctataaagat atcctcggca aaatctcccg taatcgtatg tacaccatgg
    14221 ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg tgcatacatg accatgctgg
    14281 aaccgaaata cgtcgttgct gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt
    14341 cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa catccagatg tccaacctgc
    14401 acgaaggtca gagcttcttc gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg
    14461 ccattatcgt tgccgcgatg ctgatcggct ttatcgccct gatcgccgcg ctgaacgcac
    14521 tgtttgccac cgttactggc tggtttggct acagcatctc cttccagggc atcctgggct
    14581 acatcttcta tccgattgca tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg
    14641 gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt tgcgatgatg gatctgcaga
    14701 aaattgcttc cacgctctct ccgcgtgctg aaggcatcat ctctgtgttc ctggtttcct
    14761 tcgctaactt ctcttcaatc gggattatcg caggtgcagt taaaggcctg aatgaagagc
    14821 aaggtaacgt ggtttctcgc ttcggtctga aactggttta cggctctacc ctggtgagtg
    14881 tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca tacataaaaa agccggggat
    14941 cattccccgg cttttttacg cctgttaatc agctaatggc tgcgggcgac caatcaaata
    15001 cccttgcaga tattgcaccc cgagcttatg caatagcgcc tgctgctgtt gcgtctcgac
    15061 aaactccgcg accacactca atgacttcgc tttcgccaga tcggtaattg atctcacaat
    15121 catcgcatcc agcgtattcg tgacaatatc tttcacaaag acgccatcaa ttttgatgat
    15181 atcagcctgc aaacgcttta accgttcgta gttggcatat ccggtgccaa agtcatcaat
    15241 cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg tacatgctgc tttctgcgtt
    15301 agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc tgcggggaga tgtgataacg
    15361 cttaaacaga cgaataatcc gcccggcaat attcttttgc agcagcgtga gcggcattaa
    15421 attgactgaa aagcgcggtc cttttttgtc gcaagggtgt gttgccagcc acttcaacaa
    15481 ggattccagc acttgcaaat caaaacgcgc gctaaggtta aactgagcaa taaggggcag
    15541 aaacttatcc ggggtcataa tgccgccgtc atatttcagt cgcgcgagga tctcatcata
    15601 accttcaccc tctttgttgc gaattggctg ggcgtagagc agcaaatcac cctgatctaa
    15661 cgccgtgcga atggtattca gcaatagcac ctgtttagtg gtctgcccgg aaaccatctc
    15721 ctctctgcta tccagcgcca gcacatgatg atgtgcgcag gattgctccg ccagccagct
    15781 taactgcccc aacaagggtt gcagggtttc ctgatttcca tcaaaacgcc cccaggcagc
    15841 accatagccc atatccagcc cggtattgtt ccagtgaatt tgccgactat tcaggatgtt
    15901 aaccatatgc tggagtcgcc cttccgtttc cggcccgctc agcactaaca gcagttcact
    15961 acccggcaat tgatataact tttcgttttc ctgcatcaac ggcagcagcg tacggtaaat
    16021 tgatcggata cagtgaacgc gcatcattaa gccgtaatga cgactcataa actcaagatt
    16081 atcaatgcgc aggcagcaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    16141 cggaagttgg gcaggagcgt taacggatcg gtcagcgcct gcaaatgcca gcggcgatta
    16201 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    16261 agcaccgcaa gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    16321 ttgtaattca gaaggcaaag cgttgaaaca gccctggtga gatttaaaaa cggatagcga
    16381 agcttaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    16441 aagtcgtttt cataaggtgt gcacaacaga agtagaagca cgcttaacgc tgccagccag
    16501 cttaaggtaa atgcgcgttt ctctttgccc aacgacggcg cgatatccct gcgccacaat
    16561 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    16621 ataagcacag cggtgaaaag gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg
    16681 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    16741 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    16801 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    16861 aacacgacaa acgtctggct accgagcatg accgcagtct gtaaaaaaga gagatgaaag
    16921 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    16981 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    17041 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    17101 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    17161 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    17221 caaacagggg gccagcctgt tgccgatttt tcatccaatg atgctttcac caggcggcac
    17281 taacccggac gtagactgta ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct
    17341 ttgcagcgag ggttggaaat ttggtggagc taagcgggat cgaaccgctg acctcttgca
    17401 tgccatgcaa gcgctctccc agctgagcta tagccccacg atgcgtttac gtaccaaatt
    17461 tgctgggtgc aaaatttggt ggagctaagc gggatcgaac cgctgacctc ttgcatgcca
    17521 tgcaagcgct ctcccagctg agctatagcc ccgtcacgta aagcttgtcg agttgacggg
    17581 cggcatcata tgaattccgc ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat
    17641 cgctgaaaaa tcaggcaaat gaacaatttt ggaaaaccgc tcgcattcag tagttattca
    17701 tgtcacggtt tcctgtaaag tggtgttata aaatgaacta ctaatagacc cacatacatt
    17761 cagggaattg ttatgttcaa ggagaggatg acaccagatg aacttgccag actgaccggc
    17821 tatagccgcc agaccattaa taaatgggta cgcaaggagg gctggacaac gtcaccaaaa
    17881 cctggcgtcc agggtggcaa agcccgactg gttcacgtca atgaacaggt tcgtgaatat
    17941 attcgcaatg ctgaacgtcc agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg
    18001 ccgcttgaag tattgctggt gacactggcg aaagagatga cgccagttga acaaaaacag
    18061 tttacatcct tgcttctgcg ggaagggatt atcggattgt tacaacgctt agggattcgc
    18121 gatagcaaat aatatgaaaa gattacgcaa taaaatgacc accgaagaac tggctgaatg
    18181 cctcggtgtg gctaaacaaa ctgttaaccg ttggatcaga gaaaaaggct ggaaaacgga
    18241 aaaatttccc ggcgtgaaag gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg
    18301 cgagtttatt cagaacacgc cagccttcca taacacacca atgttgatgg aagctgaaga
    18361 gagaattgct gaatacgctc ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc
    18421 gatagataat atgacagaca ttgagcaggg aaaagtcgcg caatttttgt cacgtgaagg
    18481 aattcgcaat ttcctcgccc gtctcgatat tgacgaatca gcataaataa aaaacggcag
    18541 gatattatct cctgccgttt atctttttac acgctaatta ctgctgattt tcgcgttcag
    18601 caataaaatc cagcgctttg ttgatacgct cgatactgcg ggtcttacca attgcgtgaa
    18661 cggtaacatc cagtgctgga gactgccccg ccccggttac ggcgacacgc agcggcatac
    18721 caactttacc catacccact tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt
    18781 tttcagcggt ccagtcagta atcgcggcca gtttgtcacg aaccacttcc agcggctgac
    18841 gcgctaccgg acgcagatgt tttttcgccg catcggcatc gaactcagca aaatcttcgt
    18901 agaaataacg gcagctctgt gccatctctt tcagcgtctt gcagcgttcg cccagcagtt
    18961 tcaccagatc agccagctgc gggccgttac gggtatcgat attttcctgc tcaatgtgcc
    19021 actgtaagtg agtagcaaca tactccggcg gcagcgcgtt aatgtagtga tggttcagcc
    19081 acagcagctt gtcggtgttg aacgcactgg cagatttgct gacggcattc aaagtgaagt
    19141 atttgatcat ctcttcacga gtgaagattt cctgatcgcc gtgggaccag cccagacgca
    19201 ccagatagtt cagcagtgct tctggcaaat aaccgtcatc acgatactgc attacgctga
    19261 ctgccccgtg acgtttggac agttttttac cgtcatcgcc attgatcata gaaacgtgcg
    19321 cgtaaaccgg caccggcgct ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt
    19381 tgatatggtc ttcgccacgg ataacgtggg tgatttccat atcccagtca tcgacaacca
    19441 cacagaagtt ataggttggg gaaccatcgg tacggcggat aataagatcg tccagttcct
    19501 ggttgctgaa ctcgatcgga ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg
    19561 ggttagcaaa acgtacaaca cacggttcat catcagcatg atgctcatgg ctgtggcggc
    19621 agcgaccgtc ataacgcggc ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca
    19681 ggcgctcttt agagcaatag catttataag cagtgccctc ttccagcatc tgatcgatca
    19741 ccgcgttgta gcgatcaaaa cgtttggtct ggtagtacgg accttcatcc cactccaggc
    19801 tcagccagtt catgccatcc ataatggctt cgatagcttc cggcgtggaa cgctcaagat
    19861 cggtgtcttc aatacgcagc acgaactcac cgccgtggtt acgtgcaaaa agccaggagt
    19921 aaagagcagt acgcgcgccg ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag
    19981 ttttgatttt catgaaatgg ccttacgttt agaaagatgc cgacaaccgg caaatcctgg
    20041 aaaaattgag tgggcgatat tctatcactc ccgcctgatt cctcaatgta gttcgggttt
    20101 ctaccttacg cattattgtt ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca
    20161 tcttgcttaa atttacgacg aacgaacaat ttctttagaa aatgcgttga ctcattttga
    20221 actctcccta taatgcggct ccacacagcg ggggtgatta gctcagctgg gagagcacct
    20281 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaact actttatgta
    20341 gtctccgccg tgtagcaaga aattgagaag tgggtgatta gctcagctgg gagagcacct
    20401 cccttacaag gagggggtcg gcggttcgat cccgtcatca cccaccaatt tctcgccagc
    20461 taaatttctt gtaaaaatgt gaagtaccga agtgggtgat tagctcagct gggagagcac
    20521 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac ttcgggtcgt
    20581 tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg aatcctgcac
    20641 gacccaccaa tgtaaaaaag cgccctaaag gcgctttttt gctatctgcg atactcaaag
    20701 attcgaacct gcagcaggtt tgagttgagc gcagcgaaac aacggagccg ctcgcggcga
    20761 cggcccgaag ggcgagcgaa gcgagtcatc ctgcacgacc caccaatgta aaaaagcgcc
    20821 ctaaaggcgc ttttttgcta tctgcgattt gtaagattga tttttcttct caagttcctt
    20881 gttcttctgc cgatattacc tcgtacagtg acgaggactg aacaatggcc actatctctt
    20941 caacttcaat tccttccata caaacacaga gtagtaacag ggcatcacaa ggtagcgatg
    21001 ttgcttcaaa aatcgcgcgg ataagtcagc aaatcatcaa actcactcaa caaattaaag
    21061 agattgtaga cactagcggc agtgctgaag acaaacaaaa acaggccgaa ttgattcaac
    21121 agcaaatcac cctactggaa acacaactgg cgcagttgca gagacagcag gcagaaaagg
    21181 cgcaggagaa agagcagcgt ttgtctctga atgtatccct tctaaatcct gttgaaaata
    21241 cgactcatat cgatatctat atctgataca acggagcccc ttcccgacca gccattagcg
    21301 cattgccctg gtctcgtacc acgtcccata acgcttcagc ggcggtggaa agtgaacggt
    21361 tcttcctgcg caccagcatc aactggcgtt caacaaccgg cgtaatgcgc tttaccacca
    21421 gtgggctacc ttcaggcaat ggtagcgcca gtgcgggaag aatactgatg ccgatgcctg
    21481 cggctaccat cgggaacagc gtcgccggat gtccaatctc ctgcacaata ttcgcctgaa
    21541 taccattgcg cgccagcgct gcgtcaatca gcggtcggct gcctgacgcg taatcctgca
    21601 acaccaattt cgctccctgt aatgcctgcc agggtacata atcttccacg gccaaagcgc
    21661 tatcgcgatg gcaaagcaga aagaaaggct cggaaagaat cgcttcacat tgcagatcgc
    21721 ccactgggcc tggatcgatg acgatgcaaa aatcgacatc tccctggcga atactttcca
    21781 tcacccactg ctgcggacga tcgtgcagga caaactgaat atctggatag cggcgatggc
    21841 tttcagcaat gcattgcgga ataagatgcg cggaaatggt ctggctggca gcgacccgaa
    21901 cttttccgct cagttgttgc cccatacgcc cggtatcgcg caacgtgctg ttaagttcat
    21961 ccagcagtcg ctcaagacgc aaagccaact gctgccctgc atctgtaagc accacttcac
    22021 gcgtggttct gtccagcagg cgaacaccag tatgattttc cagctccttc acactgtgac
    22081 ttactgccga ctggctcaag ccgatacgct ctcctgcacg actaaaactt ttctcctgcg
    22141 ctactgtgac gaaaaccttt aattgtttta aagaataatt catctgtttt cttcatgaat
    22201 ggatgcaata aatcaatttt atttctcaaa ctggaagaag cacaatagaa ccatcgatca
    22261 tctggagtct ttatgaaact ttttcgtatc ctcgatcctt tcaccttaac cctgatcacg
    22321 gtggtgttgc tggcctcttt ctttccggcc agaggcgatt tcgtcccctt ctttgaaaat
    22381 ctgaccaccg cagctattgc cctgctgttc tttatgcacg gcgcgaagtt gtcgcgtgag
    22441 gcgattattg ctggcggtgg tcactggcga ctgcatttgt gggtaatgtg cagcaccttc
    22501 gtgctgtttc cgattctggg tgtactgttt gcctggtgga aaccggtaaa tgtcgacccg
    22561 atgctctact ccggttttct ctacttgtgc attctcccgg ctaccgtgca gtctgcaatc
    22621 gccttcacgt caatggcggg cggtaacgtc gcggcggcgg tttgttctgc gtcagcatcc
    22681 agcctgctgg ggattttcct ttcaccattg ctagttggtc tggtgatgaa tgttcacggt
    22741 gcagggggca gccttgagca ggtcggtaaa attatgctgc aactgctgct gccgtttgtg
    22801 ttggggcatc tttcccggcc gtggattggt gactgggtgt cgcgcaataa aaaatggatt
    22861 gcgaaaactg accagacgtc cattctgttg gtggtttata cagcgttcag cgaagccgtc
    22921 gttaatggta tctggcataa agttggctgg ggatcattgc tgtttatcgt ggtggtcagc
    22981 tgcgttcttc tggctatcgt gattgtagtt aacgtcttta tggcacgccg actgggcttc
    23041 aataaggcag atgaaattac tatcgtcttt tgtggttcga aaaagagtct ggcaaatggc
    23101 atcccgatgg caaacattct gttccccaca tcggtgatcg gtatgatggt gctgcccctg
    23161 atgattttcc atcagatcca attgatggtc tgtgcggtgc tggcgcgtcg atacaaacgc
    23221 cagaccgaac agttacaggc gcagcaggaa agcagcgccg ataaagctta aagcggacgc
    23281 ttcaggggct ggaccagttg cgtcagcccc tcggttttaa tcagcagcgt gatttgcatt
    23341 agctcaccga gcttccctgc cgggaactca tctttgcggg caaaccacag cagatactcc
    23401 tccggcaggt cgattaagcg acgccctttg tatttgccaa acggcattat cgtattggct
    23461 atttcaatca gctgctcttt ttccatctca gctacccagc aaacgcagca tttccgcttc
    23521 gtcgatgact tcaatgccca gttcctgcgc cttcgccagt ttagatcctg cagcttcacc
    23581 cgctatcacc agatcggttt tcttcgacac gctgcccgcg actttcgcgc ccagttcgac
    23641 cagtcgagct ttagcgtcat cacgcgacat ctggcttaag ctgcccgtaa gcaccacggt
    23701 tttaccagca aacgggctgt caatctcttc cgcgttgata acgatcggcg caggccagtg
    23761 aacaccttcc gccaacagct cgctgatgac attgcggttg ctttcttcgg caaagaagtt
    23821 gtgaacgtgg gatgcaacga caatgccaac atcaggcacc ttttgcagct cttcaatcga
    23881 agcggcttcc agcacttcca gcgtgccgaa atatgccgcc agacctgctg cggtggcctc
    23941 gccgacttca cggatgccaa gtgcatagag gaagcgagca aaggtggttt ctttcgcttt
    24001 ttccagcgcg ttaaccacgt tttgtgccga ttttggcccc atacgctcca gtccggtcag
    24061 tttgcctgcg gtgagtttga acagatctgc cggagtgtgg acatattctt tttcaaccag
    24121 ctgatcgatg attttgtcgc ccattccgtc aacatccatc gcacggcggg aaacaaagtg
    24181 tttcagcgac tctttacgct gcgcaccgca aatcaggcca ccggtacagc gggcaaccgc
    24241 ttcaccttcc acacgctcaa cgtcagaacc acataccgga caatgcgtcg ggaatacaac
    24301 ctcacgggta tcttccgggc gttcagaaag cacgacgtta accacctgcg ggatcacgtc
    24361 gccagcgcgg cgaatcacca ctttatcgcc aatgcgtaaa ccaagacgtt cgatttcatc
    24421 cgcattgtgt aaggttgcgt tactcaccag cacgccagca acatggacag gttccagacg
    24481 cgcaacaggc gtaatcgcgc cagtacgccc aacctgaaac tcgacgtcac gcacaaaggt
    24541 catctgctcc tgcgccggga atttaaacgc taccgcccag cgcggggcac gcgcgacaaa
    24601 gccaagctgc tcctgctgtt ccagtgagtt gaccttaatc accacgccgt cgatatcaaa
    24661 gcccagcgtc gggcggtctt cttccacttt gtgatagaac gccagcactt cttccgccga
    24721 ttcacaaagc gttacccgat cgctgaccgg caacccccac tttttaaatt gcagtaaacg
    24781 gccaagatga gtatccggca gctcgccacc ttccagaaca ccaacgccat agcagaaaaa
    24841 agtgagcggt cgcttcgctg taatacgcgg atcaagctga cgcagtgaac cagctgccgc
    24901 attacgtggg ttagcaaaaa ctttcccgcc cgtgcgtcgc gcatcttcgt taatcttttc
    24961 gaaccccgcc tgcggcagga acacttcacc acgcacttcc agacgcgccg ggatattctc
    25021 tccgtgcagc ttcagcggaa tggcgcgaat agtacgcaca ttagacgtga tatcttcccc
    25081 ggtggtgcca tcgccacggg tcgcggcact gactaaaacg ccattttcat acagaatact
    25141 gacggcaagg ccatccagct tcagctcaca gcaccaggtg actttctcgt tgtttttcag
    25201 acggtcctgc acacgtttgt tgaaagcaag aaagctttct tcatcaaaaa cgttatccag
    25261 tgacagcatt ggtacttcat ggcgtatctg gctgaaagcc gccagcggcg cagcacctac
    25321 acgttgggta ggcgaatcag gcgtaatcag ttctggatgt ttggtttcca gctcgcgcag
    25381 ttcgcgcatc agcctgtcgt attcagcgtc gggaatttcc ggcgcatcca tcacatgata
    25441 aagatattca tgatggcgaa gcgtcgttcg cagttctgtc agttgttgtt cgattgattc
    25501 catatcgcac catcaatgct aaaaaccccc gacaagcggg ggttcgaaga ggagttaatt
    25561 tgccttaagt gtatcaggcg ttggcgtctt tgacttcgcg gatgatgtcc tggtactcgc
    25621 gcaatttctg cggagtcatc atacggcgct gatcgtcaag cacgacaccg cccacttcat
    25681 cggcaatatg ctgcgcagat tgcagcatca gcttgaagtt ctgcagctcg tcaccgtaag
    25741 acggtacctg cataaagata gtgacacccg gagtagtgaa atccttcatt tcaggatcaa
    25801 aggttcccgg tttcaccata ttcgccaggc tgaataacgc cgggccgctg ccatccgggc
    25861 taagatgacg atggtaaata ttcatatcgc caaaaatgaa gcccgcttgt tgaatgctgt
    25921 taagaagcag ttcaccgttt agctcgctac cgtgatgcgc cgcgacgttc atgataatca
    25981 ccgcttcttt gcgcttcggt ttatccataa ctggagccgg ttccgctaca ggctcaggct
    26041 gtggtgccgc tacgggttct gcaggctgga aagcctgttg tgccggttgc ggtgctgaat
    26101 gcacaggctg cggcgctggc gcgacctgtg gcgaaactgg ctgctgcaac ggctgttcag
    26161 gctgcggctg ataggcaggc tgctgcatcg gctgcgctgg acgcggagca tgttgcggcg
    26221 gtacctgcgc ttcaggcggc tgctggatcg gttgacgcgg ctgcgcagac gcataaggcg
    26281 gttggtactg gtgttgcggc gacggacgag cagcctcatg ctcctgagcg ttagccgggg
    26341 catgattcac gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt
    26401 cataagaatc gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag
    26461 aagatcgttc tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg
    26521 cgccaacaat gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa
    26581 caccttgcca ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc
    26641 aagtgcacta ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt
    26701 tcgaacatat cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg
    26761 ggattggtca aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca
    26821 gcggttttta ctattttgcg caaggctgga agctcgtctc gcaacctggg attcggcgtt
    26881 tcgttatttt accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct
    26941 ttacacagct cgatgtctgg atcccgactc tcatgagtta cgttccggac tggctacaat
    27001 ggctgagtta tctgttgtgg cctctggctg tcatctctgt gctgttagtg tttggctatt
    27061 tcttctccac gattgctaac tggattgccg ctccgtttaa cggtttattg gctgaacaac
    27121 tggaagcacg attgactggc gctacaccgc cagataccgg gattttcggt atcatgaaag
    27181 atgtgccgcg aatcatgaaa cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa
    27241 ttgtattact aattctttac ttcatccctg gtattgggca aaccgtcgcg ccggtactgt
    27301 ggttcctgtt tagcgcctgg atgttagcca tccagtattg cgattacccc ttcgataacc
    27361 acaaagtgcc gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc
    27421 agtttggtgc tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcatcatgc
    27481 ccgttgccgt ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcga
    27541 tgtggcggta acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt
    27601 atgccgcccc ttattccatc ttgcatgtca ttatttccct tctgtatata gatatgctaa
    27661 atccttactt ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac
    27721 agttaaggac aggccatgag taagattttt gaagataact cgctgactat cggtcacacg
    27781 ccgctggttc gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt
    27841 aaccccagct tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag
    27901 cgcggcgtgc tgaaaccagg cgttgaactg gttgaaccga ccagcggtaa taccgggatt
    27961 gcactggcct atgtagctgc cgctcgcggt tacaaactca ccctgaccat gccagaaacc
    28021 atgagtattg aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgacggaa
    28081 ggtgctaaag gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca
    28141 gagaaatacc tgctgctgca acaattcagc aatccggcaa accctgaaat tcacgaaaaa
    28201 accaccggtc cggagatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc
    28261 gttgggactg gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag
    28321 accgatctta tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggcgctg
    28381 gcaggtgaag agattaaacc tggcccgcat aaaattcagg gtattggcgc tggttttatc
    28441 ccggctaacc tcgatctcaa gctggtcgat aaagtcattg gcatcaccaa tgaagaagcc
    28501 atttctaccg cccgtcgtct aatggaagaa gaaggtattc ttgcaggtat ctcttctgga
    28561 gcagctgttg ccgcggcgtt gaaactacaa gaagatgaaa gctttaccaa taagaatatt
    28621 gtggttattc taccatcatc gggtgagcgt tatttaagca ccgcattgtt tgccgatctc
    28681 ttcactgaga aagaattgca acagtaatgc cagcttgtta aaaatgcgta aaaaagcacc
    28741 tttttaggtg cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc
    28801 ctgtcggaac tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag
    28861 gaaaagccaa agctgaatcg attttatgat ttggttcaat tcttccttta acggcataat
    28921 gtttaatgac gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattgcgct
    28981 cttcgtgcgt cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc
    29041 aggctagact ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc
    29101 aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa
    29161 aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg
    29221 cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct
    29281 ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac
    29341 tcgagtaatt tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc
    29401 aggcatttta gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga
    29461 aattgtcatt gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt
    29521 tctgagcggt cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga
    29581 aacgttcggt gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga
    29641 ggagctggag caggaaatca tagccctgat taaagataaa cacatgacag ctgacgcagc
    29701 tgctcatgaa gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct
    29761 gaaagaacgt gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg
    29821 cctgaagatt atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct
    29881 gacgccgtcc gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc
    29941 gggtggccgt acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt
    30001 gggtaccggt agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt
    30061 aaataatcag gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca
    30121 ggagcaagtg gcttctgaaa aagcagagct tgctaaactg aaagatctgc cagctattac
    30181 gctggacggt caccaggtag aagtatgcgc taacattggt acggttcgtg acgttgaagg
    30241 tgcagagcgt aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga
    30301 ccgcgacgcg ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc
    30361 gtgtggctcg caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc
    30421 atacatgaac ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc
    30481 gatggatcgt aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctcggcttt
    30541 cggtaaattg cgcattatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg
    30601 caaagagatc gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc
    30661 aattgaaatc ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc
    30721 caaagaagtt gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt
    30781 tgaccgtggt aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa
    30841 cttgatcaag caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg
    30901 tgagcttgct ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt
    30961 ctctatgagc gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga
    31021 agatgcgaag gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac
    31081 gctggttaac aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat
    31141 ttactgctta ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg
    31201 acaagaagga taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata
    31261 tcgaagacgt gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggt attgctatca
    31321 aaccaacggg taacaaaatg gtcgcgcctg tagacggtac cattggtaaa atctttgaaa
    31381 ccaatcacgc gttctctatc gaatctgata gcggcgttga actgttcgtc cacttcggta
    31441 tcgacaccgt tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg
    31501 tgaaagttgg cgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt
    31561 ctaccctgac tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt
    31621 ccggtagcgt aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc
    31681 agtgaaaaat ggcgccca
//
LOCUS       DAZSBF010000026        30162 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZSBF010000026 DAZSBF010000000
VERSION     DAZSBF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.026"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHXWD_000905"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHXWD_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR7619889.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHXWD_000906"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHXWD_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR7619890.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHXWD_000907"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHXWD_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR7619891.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHXWD_000908"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHXWD_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR7619892.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHXWD_000909"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHXWD_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR7619893.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHXWD_000910"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHXWD_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR7619894.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHXWD_000911"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHXWD_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR7619895.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHXWD_000912"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHXWD_000912"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR7619896.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHXWD_000913"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHXWD_000913"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR7619897.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHXWD_000914"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHXWD_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR7619898.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHXWD_000915"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHXWD_000915"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR7619899.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHXWD_000916"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHXWD_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR7619900.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHXWD_000917"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHXWD_000917"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR7619901.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHXWD_000918"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHXWD_000918"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR7619902.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHXWD_000919"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHXWD_000919"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR7619903.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHXWD_000920"
     CDS             complement(18516..18992)
                     /gene="rsd"
                     /locus_tag="ACHXWD_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFR7619904.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHXWD_000921"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHXWD_000921"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR7619905.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHXWD_000922"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHXWD_000922"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR7619906.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHXWD_000923"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHXWD_000923"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR7619907.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHXWD_000924"
     CDS             21688..22278
                     /locus_tag="ACHXWD_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR7619908.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHXWD_000925"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHXWD_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR7619909.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHXWD_000926"
     CDS             22750..23445
                     /locus_tag="ACHXWD_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR7619910.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHXWD_000927"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHXWD_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR7619911.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHXWD_000928"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHXWD_000928"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR7619912.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHXWD_000929"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHXWD_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR7619913.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHXWD_000930"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHXWD_000930"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR7619914.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHXWD_000931"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHXWD_000931"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR7619915.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7868 c         8310 g         6926 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agggtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg acggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca ggcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagtcgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc tagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac tcaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccaa gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaaatcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaagcgcgtc gc
//
LOCUS       DAZSBF010000027        30001 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZSBF010000027 DAZSBF010000000
VERSION     DAZSBF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.027"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHXWD_000932"
     CDS             38..580
                     /locus_tag="ACHXWD_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619916.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHXWD_000933"
     CDS             663..1364
                     /locus_tag="ACHXWD_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7619917.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHXWD_000934"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHXWD_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHXWD_000935"
     CDS             3979..5049
                     /locus_tag="ACHXWD_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619918.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHXWD_000936"
     CDS             5061..5603
                     /locus_tag="ACHXWD_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619919.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHXWD_000937"
     CDS             5611..6126
                     /locus_tag="ACHXWD_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7619920.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6828
                     /locus_tag="ACHXWD_000938"
                     /pseudo
     CDS             6092..6828
                     /locus_tag="ACHXWD_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            6939..7949
                     /gene="pyrD"
                     /locus_tag="ACHXWD_000939"
     CDS             6939..7949
                     /gene="pyrD"
                     /locus_tag="ACHXWD_000939"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR7619921.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8123..8665
                     /gene="zapC"
                     /locus_tag="ACHXWD_000940"
     CDS             8123..8665
                     /gene="zapC"
                     /locus_tag="ACHXWD_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR7619922.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="ACHXWD_000941"
     CDS             complement(8662..9771)
                     /gene="ycbX"
                     /locus_tag="ACHXWD_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR7619923.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10015..12123
                     /gene="rlmKL"
                     /locus_tag="ACHXWD_000942"
     CDS             10015..12123
                     /gene="rlmKL"
                     /locus_tag="ACHXWD_000942"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR7619924.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12135..14042
                     /locus_tag="ACHXWD_000943"
     CDS             12135..14042
                     /locus_tag="ACHXWD_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7619925.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISHLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQMADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14172..15425
                     /gene="pqiA"
                     /locus_tag="ACHXWD_000944"
     CDS             14172..15425
                     /gene="pqiA"
                     /locus_tag="ACHXWD_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR7619926.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15430..17070
                     /gene="pqiB"
                     /locus_tag="ACHXWD_000945"
     CDS             15430..17070
                     /gene="pqiB"
                     /locus_tag="ACHXWD_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR7619927.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17067..17630
                     /gene="pqiC"
                     /locus_tag="ACHXWD_000946"
     CDS             17067..17630
                     /gene="pqiC"
                     /locus_tag="ACHXWD_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR7619928.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17886..18053
                     /gene="rmf"
                     /locus_tag="ACHXWD_000947"
     CDS             17886..18053
                     /gene="rmf"
                     /locus_tag="ACHXWD_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR7619929.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="ACHXWD_000948"
     CDS             complement(18123..18641)
                     /gene="fabA"
                     /locus_tag="ACHXWD_000948"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR7619930.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18710..20470)
                     /locus_tag="ACHXWD_000949"
     CDS             complement(18710..20470)
                     /locus_tag="ACHXWD_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7619931.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20656..21108
                     /gene="matP"
                     /locus_tag="ACHXWD_000950"
     CDS             20656..21108
                     /gene="matP"
                     /locus_tag="ACHXWD_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR7619932.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21184..22224)
                     /gene="ompA"
                     /locus_tag="ACHXWD_000951"
     CDS             complement(21184..22224)
                     /gene="ompA"
                     /locus_tag="ACHXWD_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR7619933.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22579..23088)
                     /gene="sulA"
                     /locus_tag="ACHXWD_000952"
     CDS             complement(22579..23088)
                     /gene="sulA"
                     /locus_tag="ACHXWD_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR7619934.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23307..23936
                     /gene="sxy"
                     /locus_tag="ACHXWD_000953"
     CDS             23307..23936
                     /gene="sxy"
                     /locus_tag="ACHXWD_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR7619935.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23899..26061)
                     /gene="yccS"
                     /locus_tag="ACHXWD_000954"
     CDS             complement(23899..26061)
                     /gene="yccS"
                     /locus_tag="ACHXWD_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR7619936.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26071..26517)
                     /locus_tag="ACHXWD_000955"
     CDS             complement(26071..26517)
                     /locus_tag="ACHXWD_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR7619937.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26640..28694
                     /gene="helD"
                     /locus_tag="ACHXWD_000956"
     CDS             26640..28694
                     /gene="helD"
                     /locus_tag="ACHXWD_000956"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR7619938.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISSQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28726..29184)
                     /gene="mgsA"
                     /locus_tag="ACHXWD_000957"
     CDS             complement(28726..29184)
                     /gene="mgsA"
                     /locus_tag="ACHXWD_000957"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR7619939.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29280..29942)
                     /locus_tag="ACHXWD_000958"
     CDS             complement(29280..29942)
                     /locus_tag="ACHXWD_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR7619940.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7734 a         7084 c         7877 g         7306 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattccaggg tgtacaactc actaacccaa cgccttacta cattaacctg
     6661 attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagttgtgcc gcctaaaagc
     6721 cagcgtcaga caagctggtg tcaggcaatc gcaccgtgtc atgtcgcctg gcgggcgata
     6781 aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa caggttcgga
     6841 aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact cgcacataat
     6901 acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc ttcgttcgta
     6961 aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag caattacgcc
     7021 gtattacagg aacgccgttt gaagcactgg tgcggcagaa agtgcctgcg aaacctgtta
     7081 actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt gataaagacg
     7141 gggagtgcat tgacgcgtta ggcgcgatgg gatttggatc gatcgagatc ggtaccgtca
     7201 cgccacgtcc acagccaggt aatgacaagc cgcgtctctt tcgtctggta gatgccgaag
     7261 gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta gagaacgtaa
     7321 aaaaggccca ttatgacggc gtcctgggta ttaacatcgg caaaaataaa gatacgccag
     7381 tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc tatgcgggat
     7441 atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg caatatggtg
     7501 aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg caagcgatgc
     7561 accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa gaagaattga
     7621 tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca accaatacca
     7681 cactcgatcg ttctcttgtt cagggaatga aaaattgcga tcaaaccggt ggcttaagtg
     7741 gtcgtccgct tcagttaaaa agcaccgaaa ttattcgccg cttgtcactg gaattaaacg
     7801 gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg cgtgaaaaga
     7861 ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa ggtccgccgc
     7921 tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt tataagataa
     7981 tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat
     8041 tttgtacttt tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt
     8101 ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta
     8161 cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg
     8221 ttttgcgcgc aagatgttga ctccagatgc cttttctccc gcaggctttt gcgttgacga
     8281 cgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca
     8341 aaaagccgaa ctggtgctga atgcactggt agcaattcgc tatctcaaac cgcaaatgcc
     8401 gaaaagctgg cattttgttt cccatggtga aatgtgggtg cccatgccgg gcgatgcggc
     8461 ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga
     8521 aaatgccgca ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca
     8581 gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag
     8641 cttcagcctc gaacaggcag tctaacgcgc caacttaagt gcagtcttcg gtacacagct
     8701 acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggca tgacttcgcc
     8761 ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg
     8821 aattccctga ttttctaatt gctccagcaa cacctgttgg ttatttccac gaaatgcctg
     8881 tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc
     8941 agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc
     9001 aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt
     9061 atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg
     9121 ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa
     9181 caccacatca ccaatgcgaa tcactttcca gcgatcttct tcccaggctg acgcgccgga
     9241 aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag
     9301 atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagagg
     9361 tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac
     9421 ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt
     9481 aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac
     9541 atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt
     9601 aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat
     9661 cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat
     9721 gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca cggtgcggtc
     9781 ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct ataatgcgca
     9841 acaattttct tagcgcctgt cccattaggc tattttattt gtcagtttgg ccctgggttg
     9901 tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga
     9961 ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat
    10021 tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaac
    10081 ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg
    10141 cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc
    10201 gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca
    10261 gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc
    10321 cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg
    10381 aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg
    10441 ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt
    10501 ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg
    10561 atgcgaaccg gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg
    10621 ttgctgattg aagcggcgat gctagcgacc gatcgcgcac caggcttgca ccgtgggcgt
    10681 tggggcttta gcggctgggt gcagcatgat gaagctatct ggcaggaagt gaaagcggaa
    10741 gcgcaaactc gtgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac
    10801 agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa
    10861 ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg
    10921 tacggcacag tgttgagcaa cccgccatac ggggaacgtc tggacagcga accggcgctg
    10981 attgcgctgc atagcctgct gggtcggatc atgaaaaacc agtttggtgg ctggaatctc
    11041 tctttgttta gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc agacaaacag
    11101 tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc
    11161 actccagaca gcaaaccggc gatggtagcg gaagactacg ccaaccgcct gcgtaagaac
    11221 ctcaaaaaat tcgagaagtg ggctcgccag gaaggaattg aatgttaccg cctgtatgac
    11281 gccgatctac cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag
    11341 gagtatgcgc cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct gttcgatatt
    11401 atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt
    11461 gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga gtttcttgaa
    11521 gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga tactggtctg
    11581 ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg caaagatttc
    11641 ctcaacctgt tctcttatac cggtagcgcc accgtgcatg cgggattagg cggcgcgcgc
    11701 agcaccacca ccgtggatat gtcgcgtact tatctggagt gggcagaacg caacctgcgt
    11761 ctgaatggcc tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg
    11821 cgtgaggcaa atgaacagtt tgatctgatc tttatcgatc cgccaacctt ctctaactca
    11881 aaacgaatgg aagatgcgtt tgatgttcag cgcgatcata tggcgctgat gaaagatttg
    11941 aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg tggcttccgt
    12001 atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg
    12061 ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc
    12121 tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg ttcagcgacg
    12181 cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt tgtctggtgg
    12241 gtcgcaacgg cgcgggcaaa tcgacgttaa tgaaaatcct caaccgtgaa caagggctgg
    12301 atgacggtcg cattatttac gagcaagatt tgattgtagc tcgtctgcaa caggagccgc
    12361 cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa gaacaagcgg
    12421 aatatctgaa acgctatcac gatatttcgc acctggtgat gaacgacccg agcgagaaaa
    12481 atctcaacga actggcgaag gttcaggaac agttggatca ccacaacctg tggcagctgg
    12541 aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaatgtt gcgctgtcgt
    12601 cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg agtaatccgc
    12661 gcgtgctgtt gcttgatgaa ccgacaaacc acctggatat tgaaaccatc gactggctgg
    12721 aagggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac cgttcgttta
    12781 tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg acctatccag
    12841 ggaattacga ccagtacctg cttgaaaaag aagaagccct gcgcgtggaa gaactacaaa
    12901 atgccgagtt cgatcgcaaa ctggcgcagg aagaggtgtg gatccgccag gggatcaaag
    12961 cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcaatgcgt cgcgagcgtg
    13021 gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc agccgctccg
    13081 gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt tgacggtaag caactggtga
    13141 aagatttttc ggcccaggtt ctacgtggcg acaaaattgc cctgattggt ccgaatgggt
    13201 gcggcaaaac cacgctgctg aaactgatgc tcgatcagct tcaagcggac agcgggcgta
    13261 ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg gaactggatc
    13321 ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg gttaacggca
    13381 agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa cgggcgatga
    13441 cgccggtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg cgtttgttcc
    13501 tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat gtcgaaacgc
    13561 tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg gttagccacg
    13621 atcgtcagtt tgtcgataac accgttacag aatgttggat cttcgaaggc ggcggtaaaa
    13681 ttggtcgtta tgtcggcggt tatcatgatg cccgtggtca gcaagagcag tatgtggcgc
    13741 tcaaacagcc tgcggtgaaa aaaaccgaag aagccgccgc ggcaaaagca gaaactgtaa
    13801 aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag ctaccgcaat
    13861 tgctcgaaga tctggaggcg aagctggaag ccctacagac gcagatggcg gatgcttcct
    13921 tcttcagtca gccacatgag cagacacaaa aagtgctcgc cgatatggct gctgcagagc
    13981 aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa aatggtggct
    14041 gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg cctgatgaac
    14101 tgggtttatc aggcatgaaa accaacactt tgttagccat ctgtaaaata gcgcatcatt
    14161 aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc tcgcagtgtg
    14221 acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt ccccggtgtg
    14281 gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tccgaccgcc tatgcgttgg
    14341 ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg aacgttgcag
    14401 gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct gaggactacg
    14461 ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt tgtctgataa
    14521 ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag caactggcac
    14581 gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgct ggtgtgctgg
    14641 tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc tttctcccct
    14701 ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt cgctggttat
    14761 gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc gtcacgggga
    14821 tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct gatgaacccg
    14881 tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagccag cagtggacac
    14941 tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg cccatcatgg
    15001 tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc attctgttat
    15061 ggagcgaagg ctcttatccc gtcgctgcgg tgatctttct ggccagtatt atggtgccaa
    15121 cgttaaagat gatcgccatc gcgtggctgt gttgggatgc caaagggcat ggcaagcgcg
    15181 acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc tggtcgatga
    15241 ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt ttaatgagta
    15301 tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca atgttttctg
    15361 ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag catgaggagt
    15421 cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa gaactggtct
    15481 cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct tttttatcat
    15541 tacagccatc agggactgga agtgaccctg atcaccgcga atgcggaagg aattgaaggt
    15601 ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag cgccacactg
    15661 gctgatgatt tgacgcacgt tgaaatcaaa gtgcggctga attctggtat ggaaaaattg
    15721 ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga agggattagc
    15781 ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc gaaaggcagc
    15841 aaaatggata aatacgattt gctggactcg ccaccgttgg ccccgcctga tgcgaaaggt
    15901 atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga tccggtgctg
    15961 ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca aaaacgcaat
    16021 atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag caatgttcgc
    16081 ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg tgtggagatg
    16141 ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga aggtctggat
    16201 ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca gaagagcatt
    16261 caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga ttcggtacgc
    16321 ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac cgtaagcaaa
    16381 gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg tattccggta
    16441 ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc ggatgttgtt
    16501 gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac cggaaacctg
    16561 gtcactggtg cactgtatgt tgatcttgat ttctatccaa atacgcctgc aataaccggt
    16621 attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct ggcgcaaatc
    16681 cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa tccgatgatt
    16741 gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct gcaaacgacg
    16801 ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc gacggatatg
    16861 cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg ctccgcagcc
    16921 tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga actgcaaccg
    16981 gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa ggacaaaaaa
    17041 gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt gcagcactgt
    17101 ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta cctgtggtgc
    17161 agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta gagcaggtca
    17221 ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat gtgaagtatg
    17281 tgattgccaa caacaacttg tgggccagcc cgttggatca acaattgcgc aacaccctgg
    17341 ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct ctgggaagcg
    17401 cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat ggcaaggtca
    17461 ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt ccgttccgtc
    17521 tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtactg gccagtgtct
    17581 ggagtcagga agccgcttct attgcgcaag agataaagcg tctaccttaa ttataaagat
    17641 ttgtaaatat aaccgtctcc ggtatgttgc ctgaggcggt ttttttgttt ctaacgtgcg
    17701 gaaaaagttg ttcctcttta cattttttgt acaaccgaca tacccgtgta gctcacaaat
    17761 atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta ttgcttaact
    17821 gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta tgagggaaac
    17881 gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt ggttatcagg
    17941 ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat caaaggtcac
    18001 aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc tgattctgtc
    18061 tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct gtctgattat
    18121 aatcagaagg cagaagtatc ctggaacaga ccgactttca ggtcgctggc ggtatagatc
    18181 aaacgaccat caaccagcac ttctccatcc gccaggccca taatcagacg acggttaaca
    18241 atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac ctgaccagtg
    18301 aatttcactt cgccaacgcc cagtgcgcgg cctttacctt cgccgcccag ccagccgagg
    18361 tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat aaccggatcg
    18421 ccaataaagt ggcatccgaa gaaccacaga tccggattga tatccagttc tgcttcaaca
    18481 taccctttgt cgaagttacc acccgtttcg gtcattttga ccacacggtc catcatcagc
    18541 atattcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgcg accagaggca
    18601 agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt aagccttatt
    18661 ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat cagttcggaa
    18721 taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc tgttgcgatg
    18781 cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg ccatcccaca
    18841 ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag atggtgaatt
    18901 tgccttcttc taccgctttc actagttcac tgtgaagact taaatggcga acgttagctg
    18961 tggggataat gacaccttgt ttcccggtta actcacgttg ctggcaaata gcaaagaagc
    19021 cttcgatttt ctcatttaaa ccaccgaccg gctgggcgcg accgaactga tcgactgaac
    19081 ctgtgatagc gatactctga ttcaccggca catcggcgag ggcgcttatc agggcgcaga
    19141 gttcagccat cgaggcacta tctccatcaa cttcactgta tgactgctca aatgtcagcg
    19201 atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac gcttgcatga
    19261 tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg atgtcggtga
    19321 attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca aaagcgcgtg
    19381 gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt tcgccttcgg
    19441 tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg aggaaacctt
    19501 cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag gtgtcgccat
    19561 cacacaggga ggcgacctct ttacactggc ggaggatcca ctgcgggcta agcggaagtg
    19621 tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccag gcatccgctc
    19681 ccggtgcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac cactgggtta
    19741 ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata gcctgctctg
    19801 aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct accagaatga
    19861 ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac tcatcaaacg
    19921 caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac agcagaggtt
    19981 gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga tgcaccagac
    20041 caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt tgctccgctt
    20101 ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct gcactcactg
    20161 cgtgacgtaa gcggatggag tggccggaaa cctcatagtg accgcccacc agttggcctg
    20221 catcgctttg tagcgtacgc gcggcattgg caataagatt cagatactca gactcttccg
    20281 gggccttcgc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc tccagcgcaa
    20341 attgcagccg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc aaatgtggct
    20401 gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat gcaagtttcg
    20461 taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata ccgtaaccgg
    20521 cactacagca cacgtagaaa gcaccgacaa tactccaggc atgggcgtta aagctcacag
    20581 gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga gttacacggt
    20641 aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg ttggaaatgg
    20701 aagtatctgg tgaagaagca tcgcgaaggg gagttaatca cccgttacat agaagccagt
    20761 gccgcccagg aagccgtcga tgtgttgctc tcgctggaaa atgaacccgt gctggtaaat
    20821 ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca aaccattcgg
    20881 gcaagacgaa agcgccattt taatgcggaa caccagcata cgcgcaaaaa atcgatcgat
    20941 ctggaattta ttgtctggca acgtctggct ggtcttgcgc agcgtcgcgg taaaacgctt
    21001 tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata cgcgaataaa
    21061 atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact gacgaaagtc
    21121 agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct accagacgag
    21181 aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga tctctacgcg
    21241 acgatccgga gccaggcagt cgatcagtgc agcacgctgt ttcacgttgt cacaggtgtt
    21301 gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg ccgggatacc
    21361 tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac cctggttgta
    21421 agcgtcagaa ccgatgcggt cggtgtaacc cagaacaact acggaaccgt ctttcggatc
    21481 caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg gtttcagggt
    21541 tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg tctgtacttc
    21601 cggtgccgga gctggagccg gagcaactac tggagctgct tcgccctgac cgaaacggta
    21661 ggaaaccccc aggctcagca tgccgttgtc cggacgagtg ccgatggtgt gtgcgtcacc
    21721 gatgttgttg gtccactggt attccagacg ggtagcgatt tcaggagtga tcgcgtactc
    21781 aacaccgcca gcgaagaccg gagaaacgcc ggtgtcgtgg tttttaccat aaacgttgga
    21841 tttagtgtct gcacgccata ccataccacc cagacgagtg tagatgtcca ggtcgtcagt
    21901 gattgggtaa cccagtttag cggtcagttg aacgccctga gctttgtatg caccgttttc
    21961 aacgctgcct ttgtacggca tacgacctaa ccagtcgtaa cccatttcaa agccaacata
    22021 cgggttaacc tggtaaccac caaaagcacc agcgcccagt tggttttcat gggtcgggcc
    22081 attgttgttg atgaaaccag tgtcatggta ctgggaccag cccagtttag caccagtgta
    22141 ccaggtgtta tctttcggag cggcctgcgc tacggtagcg aaaccagcca gtgccactgc
    22201 aatcgcgata gctgtctttt tcattttttg cgcctcgtta tcatccaaaa tacgccatga
    22261 atatctccaa cgagataaca cggttaaatc cttcaccggg ggatctgctc aattaactct
    22321 accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca acgtagttga
    22381 aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat aaggtatgtt
    22441 taatcttttt tgtcagcgac aatttacaga agaaaatcgc ggaaaccgct tcagacaagc
    22501 ctccgcaagg aaaattagtc acgactgaaa gcattggctg ggcgacaaaa aaagttccag
    22561 gattaatcct aaatttactt aatgatacaa attagagtga atttttagcc cggaaagttg
    22621 tctcgtggcg tgagaggatg cgcttaccgg acgcataata aaccccatag cgttaccttc
    22681 atttgccgca tcaacaagtt cagcatgctc ttcttcagtc aaatcatctg ccaaccaacc
    22741 gatcaccaca ctgtaattgc ccgtgcgtaa agcgcgaacc attgactcca cagtgtggca
    22801 aggggagagc tggctaatct gcattacttt cgttaagggt agcccagatg cctgaaccca
    22861 ttcccgactc agtttttgtt gcggtgttaa ccagagttgc cagcgcgatt gctgaccgag
    22921 ttgctgtaac aatggcaaca gtagaagttg cgtcatcatg ggctgatctt cgcgatagac
    22981 aacttcactg ataagcccgg ctgtagtgtt ttccgtagag acacgcgcaa ttttacttgc
    23041 tgcggatgag aacgacgaag aacgatgtgc atagcctgaa gtgtacataa tcaatccagc
    23101 ccctatgagt tactgtatgg atgtacagta catccagtga caacaaagat caaccctatt
    23161 ttcggaaaga gcctcgcaaa ttttgtcgtt ggtgacggga aaacataaat taatcttgcc
    23221 ccttaagaat aagttgccta ttttcgtagt taacggatcc gttaatgtga atcattcttt
    23281 tatgttatga ttttaaaagg aattttatga aaagcctctc ctataagcgg atctataaat
    23341 cacaagaata cctggcaacg ttgggcacaa ttgaataccg atcattgttt ggcagttaca
    23401 gcctgaccgt tgacgacacg gtgtttgcga tggtttctga tggtgagttg tatcttcggg
    23461 cttgtgagca aagtgcacag tactgtgtaa aacatccgcc tgtctggctg acatataaaa
    23521 agtgtggccg atccgttacc ctcaattact atcgggttga tgaaagtcta tggcgaaatc
    23581 aactgaagct ggtgcgtctg tcgaaatatt ctctcgatgc agcgctgaaa gagaaaagca
    23641 cgcgcaatac ccgggaaaga ctgaaagatt tgcccaatat gtcttttcat ctggaagcga
    23701 ttctcgggga ggtggggatt aaggatgtac gggcgttacg tatacttggg gcaaaaatgt
    23761 gttggttgcg actgcggcag caaaacagtc tggtgacaga aaagattctg tttatgcttg
    23821 aaggtgccat tatcggcatt catgaagctg cgctcccggt ggcacgccgc caggagcttg
    23881 cagaatgggc tgactctctt acgccgaaac aggagtttcc tgcggaactt gagtaatctg
    23941 gcgttgcaga cgaccaatct caggcagtaa tgcaatcaat aatccaactt gttgtacgac
    24001 cagaggttct ttgctgtctg cccgtggttc aagttgctgc atccgctgtt tcaggctagc
    24061 taatgcctca ttgacgcgtt cttcatcagc aggttgatga tgtaacgcgt catcaacata
    24121 gcaaactgcg tcatcaagaa acgccagaat ttcaggatta gttaactgct cccggtgagc
    24181 accgagggct gagatatagc tggtaaacgt atggttaagg cacagcaacc gaaacgcggc
    24241 ttcgcgaatt tgcggggtaa cgttcggctc gctggacata tttgatacca ccgacgccag
    24301 ctcagcatca cggttgtgtg catcgcggcg ggcaatacga tacgccagac ggttatcacg
    24361 cccctgatgg tattgctcca gtatggcatc gagataccga cagttggcct ctgtggcgcg
    24421 ttcgagcatg cgcggcagat tgcgaaactg ccagtcaggc cagatgtagc tcactgccgc
    24481 ccacgcaatg gcacaaccaa tcagcgtatc gattacgcga ggtaacgcta cttcaaaacc
    24541 ttcacccagt aagttaaaac acagtagcac caaaagtgtg atgaacatcg ttgcatgagc
    24601 gtattgcacg ttacggaagg caaaaaagag cacgccggta ataaccagca gcaccagctg
    24661 cccttccagt gatggcacaa accacagcac aggaatgcca atggcgatac ctaccagcgt
    24721 accaataatc cttaacttca ggcggtggcg cgtggcgtta tagtttggct ggcagacaaa
    24781 caaacttgtc agcaagatcc aatacccgtg atgcattccg gttatctgaa tgatggcgta
    24841 gccgaagcac aataccagcg acattcttac cgcatgacgg aagagggcgg attccggcgt
    24901 gaagtgacgg ctaagacgca gccagatatc actcaacccg tgcgggctgt catcagcgag
    24961 ctcattttcg tcattattat ggggtagtgc ctgggcctgt tctgattcaa ttgttgccag
    25021 ttgggcatca atggcgcgta aattgttcag caaaaatccc agtgttttga gtaaatcggc
    25081 gggtgcgccg ttatcgcgca tccgctccag cgcagcatca atatgcgtaa aagcgcgctc
    25141 aaaatgcgga tcatgttgat aaggctgacg caacaaaata cagcgtgaca gttgctggca
    25201 cgcctggccc tgcatcgaca tcagccgctg aaaacggaac agcacgtcgc tgtggcgaaa
    25261 atgttcacgc aatgtttgat actgaatatg agaagagctg gcacgctcgt gaatatcctg
    25321 tgcgacaaag taataatgca gcgtgcgacg cgttccccgt tgaccacgat cgccacgtaa
    25381 gcgggtcagc agcgagagtt tcgtctgatt caatgtcgcc atcagcagac cgttggcgag
    25441 agccaaatcg tacagcggtg cctggctttc atcttcaata tcaggatcaa acatgcgcga
    25501 cttgagctca agataacgcg ccagttgttc atagcaacgc gccaggttgt cctgcagcgg
    25561 gcggaccggg aacagcagat gaccaataag tgtcaggacg ttgtaccaga cggcaccggc
    25621 cagcagatac atcggctgct gataccagtg ctcatacagt gatgttccca acatagtgta
    25681 aatggcgatc agcaatgcac cgaaggcaat tgttgcatag cgttgaccca gaccgccgag
    25741 caaaatgaag ccgctggtag agagcgttaa gccaatcgca aatagccagg gccagggaaa
    25801 cagcaattct actgaggccg aggcgataaa aaagcagaac agcgtaatga tgaggttacg
    25861 caaacgtccc gccagtcggt catcgagatc ggtcagcgcc gctgccacca tccccagcgt
    25921 tagcggaatc gtcagtttta catcacccag ccaccacgga aacgctgtgg ttccacaaag
    25981 cgcaataaaa atacgcgcgt aatacagcca ggcgctgttc caggtatagc gtttgagcaa
    26041 aggacttagc ataaaggcca tattagtgga ttattcaaaa cgacgacgtg cattggcttc
    26101 acgcgcagct tgcgctgttt ctaccgatac cacgcgacga ccaaccggcc atagtgcaat
    26161 agcggcaatt ttaaagttcg caatgccgac aggaatgcca atgattgaaa tacattgtgc
    26221 gatgcccgtt gcaatgtgca tcaggcataa ccaccagcca aagaaaatca gccagaaaat
    26281 attcaatacc gtaccgccag tattcagcag cacatttttg ccagccgggt tcagttcatc
    26341 gacatgaata gcttcattgc cataaggcac cagagatagt ttagtgatct cccagcagga
    26401 tcgtgtcagc ggtaaggtaa aaatcagcac aatactgacc agagtcgcca acagccagcc
    26461 cagagtggtg gcaaatccgc caagcacaaa gttcagaatg ttcaaaacgg tacgcataaa
    26521 acctcgcttt actgtggttt tcagtaatgg gcggcaattg taacgttttt ttgggctgga
    26581 gcacgttttc tctgacggtt acactgataa gaaataattt cgtgtggatc tacagagtca
    26641 tggaactgaa agcgacaacg cttggaaaac gtctggcaca gcacccttac gatcgggcgg
    26701 tgatcctcaa tgccgggatt aaagtctccg gcgatcgcca cgaatacctt attcctttca
    26761 atcaattact ggcgattcac tgtaagcgcg gtctggtatg gggcgagctg gaatttgtac
    26821 tgccggacga aaaagtggtg cgtctgcacg gcaccgaatg gggcgagacg cagcgttttt
    26881 accatcatct tgatgctcac tggcggcggt ggagtggcga gatgagcgaa attgcgtctg
    26941 gtgttttacg ccagcaactg gatttgattg ccacgcgcac tggagaaaat aaatggctga
    27001 cgcgtgagca aacctctggc gttcagcaac aaatccgcca ggctttgtcg gcgttgccgt
    27061 tgccggttaa ccgactggaa gaattcgata actgccgtga ggcgtggcgt aaatgtcagg
    27121 cctggttgaa agatattgaa agcgctcggt tgcagcataa ccaggcgtat accgaagcca
    27181 tgcttaccga gtatgcggat tttttccgcc aggtcgagtc ttcaccgctg aatccggcgc
    27241 aggcccgggc agtcgttaat ggcgagcatt ctctgttagt gctggcaggt gcaggaagcg
    27301 gaaaaacgtc ggtgctggtg gcccgtgcag gctggttgct ggcgcgtggt gaagcgtccc
    27361 ctgagcaaat tttattgctg gcgtttggtc gcaaagccgc tgaagagatg gacgagcgga
    27421 ttcgcgaacg gctacatacc gaagacatta ccgcacgcac gtttcatgcg ctggcgctgc
    27481 atattattca gcagggcagc aaaaaagttc cgatagtcag caaactggaa aatgataccg
    27541 ctgcccgtca tgaactcttt attgctgagt ggcgcaagca atgcagcgaa aagaaagcgc
    27601 aggcgaaggg ctggcggcaa tggctgacgg aagaaatgca gtggtcagtg ccagaaggta
    27661 acttctggga tgatgaaaaa ttacagcgtc gccttgcctc tcgcctcgat cgctgggtaa
    27721 gtctgatgcg gatgcacggt ggtgcacagg cagaaatgat tgccagtgca cccgaagaga
    27781 ttcgcgatct gttcagtaaa cgtatcaagt tgatggcccc gttattaaaa gcctggaaag
    27841 gtgcgctgaa ggcagaaaac gctgtcgatt tttcgggcct tattcatcag gcgattgtga
    27901 ttctggagaa aggtcgcttt atcagcccgt ggaagcatat tctggttgat gaatttcagg
    27961 atatctcgtc gcagcgggca gcgttgttag cggcattacg caagcaaaac agtcagacga
    28021 cgttgttcgc tgttggtgat gactggcagg cgatttaccg attcagcggt gcgcaaatgt
    28081 cgctcaccac cgctttccat gaaaactttg gtgaaggcga acgttgtgat ttagacacga
    28141 cttaccgttt taacagtcgt atcggtaagg tggcaaaccg gtttattcag cagaacccag
    28201 gccagctgaa aaagccgcta aacagcttaa ccaatggaga caaaaaagcc gtcacgttat
    28261 tggatgagag tcaacttgac gctttgctgg ataagctctc tggttatgcc aaaccggaag
    28321 agcgcattct gatcctggcg cgttaccatc acatgaggcc tgccagcctg gaaaaagcgg
    28381 caacacgctg gccgaagttg caaatcgact ttatgaccat tcatgccagc aaagggcaac
    28441 aggcggatta cgtcatcatc gttggcttgc aggagggaag tgatggtttt ccggctgcgg
    28501 cgcgggagtc gattatggaa gaggcgctac tgccaccggt tgaggatttc ccggacgctg
    28561 aagaacggcg gttaatgtac gtggcgctga cccgggcacg ccatcgggta tgggcactgt
    28621 ttaacaaaga gaatccctct ccctttgtgg aaatactgaa aaatctggat gtgccggtgg
    28681 cgagaaaacc gtaagaaaca ggtggcgttt gccacctgtg caatattact tcagacggtc
    28741 cgcgagataa cgctgataat cggggattag aatatcgacc gcgtcgttga aatgcggcga
    28801 ctggattatg aagtctgccg ttgccacgtt ggtggcgacc ggaatgttcc ataccgtcgc
    28861 cagacgcagc aaggctttca cgtcaggatc gtgcggcacg gcatttagtg gatcccagaa
    28921 gaaaatcaat acatcaattt tcccttctga gatcaatgcg ccaacctgct ggtcaccccc
    28981 cattgggcca ctcaacatcg cgttgacgtt catgccggtc gcgcgggaaa ttaagttacc
    29041 ggtagtgcct gttgcataca gtacgtgttg ttccagtaac ggttgatgcc gttccaccca
    29101 gctcatcagc atttgtttgc agtgatcgtg tgccaccagc gcaatatgtt tccgcgcagg
    29161 taaagtgcga gtcgtcagtt ccataatgta catccgtagt taactttcct acagattact
    29221 gtaagcactt atcgctgcaa gataaagacc gaaaaagcct gcgcacaggc acaaaaatct
    29281 caggaagatg gttgtttttc cgcccactgc aggaaagtat ttcgcgtttg tgggtcagcc
    29341 agtttaaacc aatacttcag ccgttgttct gtgagcacct gagactgcgg tggaatagca
    29401 tccagctcgg aaacacccga aagcagcgta ctgtcatctg ccatcatcgt ggcaaattgc
    29461 ggtagtgaag cgcgtttcgc ggatttgtta tagcgttcaa cctcgacctc ataatcaatc
    29521 gtttttgccg ttgaggtaat ggcgagaata tccagcttta ccggaatcgg cgtcgcatcg
    29581 ccatccaaca attcaaggcg cggcgcggca tcaaaatggt tagcttcccg ctcattctcc
    29641 aggcgaggca ggcgaaaatt gacctggttg atgagctggg tattaaaact gaccaccagc
    29701 ggtggggaga tatacagccg ttcttcacta ttggaaagat gaatcgtctt ctcaacgcga
    29761 aacactaact gatgcggtcc attatccagt tcaatgctgt ccgcaccgcg cagcagagag
    29821 ctggaaacct tcttgccgtc gagtaccaga agatcgacat cggttgaaag ccgcagcgtg
    29881 gtggcaaaaa cggataccgg caaacataat gcaattaagg tggtcacgat gccggttttc
    29941 ataggtagct cctgtcaaaa gaccgcccga ataataatgt gtcaaaattt ttctttaatc
    30001 a
//
LOCUS       DAZSBF010000028        29548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZSBF010000028 DAZSBF010000000
VERSION     DAZSBF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.028"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHXWD_000959"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHXWD_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR7619941.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHXWD_000960"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHXWD_000960"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR7619942.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHXWD_000961"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHXWD_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR7619943.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHXWD_000962"
     CDS             complement(3644..4030)
                     /locus_tag="ACHXWD_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR7619944.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHXWD_000963"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHXWD_000963"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR7619945.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHXWD_000964"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHXWD_000964"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR7619946.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHXWD_000965"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHXWD_000965"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR7619947.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHXWD_000966"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHXWD_000966"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR7619948.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHXWD_000967"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHXWD_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR7619949.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHXWD_000968"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHXWD_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR7619950.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHXWD_000969"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHXWD_000969"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR7619951.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHXWD_000970"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHXWD_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR7619952.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHXWD_000971"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHXWD_000971"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR7619953.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHXWD_000972"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHXWD_000972"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR7619954.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHXWD_000973"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHXWD_000973"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR7619955.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHXWD_000974"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHXWD_000974"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR7619956.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHXWD_000975"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHXWD_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR7619957.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHXWD_000976"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHXWD_000976"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR7619958.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHXWD_000977"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHXWD_000977"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR7619959.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHXWD_000978"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHXWD_000978"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR7619960.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHXWD_000979"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHXWD_000979"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR7619961.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHXWD_000980"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHXWD_000980"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR7619962.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHXWD_000981"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHXWD_000981"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR7619963.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHXWD_000982"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHXWD_000982"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR7619964.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHXWD_000983"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHXWD_000983"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR7619965.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZSBF010000029        28520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZSBF010000029 DAZSBF010000000
VERSION     DAZSBF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.029"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(288..515)
                     /locus_tag="ACHXWD_000984"
     CDS             complement(288..515)
                     /locus_tag="ACHXWD_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR7619966.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            707..1912
                     /gene="csdA"
                     /locus_tag="ACHXWD_000985"
     CDS             707..1912
                     /gene="csdA"
                     /locus_tag="ACHXWD_000985"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR7619967.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            1912..2355
                     /gene="csdE"
                     /locus_tag="ACHXWD_000986"
     CDS             1912..2355
                     /gene="csdE"
                     /locus_tag="ACHXWD_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR7619968.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2406..3212)
                     /gene="tcdA"
                     /locus_tag="ACHXWD_000987"
     CDS             complement(2406..3212)
                     /gene="tcdA"
                     /locus_tag="ACHXWD_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR7619969.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3289..4386)
                     /gene="mltA"
                     /locus_tag="ACHXWD_000988"
     CDS             complement(3289..4386)
                     /gene="mltA"
                     /locus_tag="ACHXWD_000988"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR7619970.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            4595..4671
                     /locus_tag="ACHXWD_000989"
     tRNA            4595..4671
                     /locus_tag="ACHXWD_000989"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4629..4631,aa:Met,seq:cat)
     gene            complement(4746..5999)
                     /gene="amiC"
                     /locus_tag="ACHXWD_000990"
     CDS             complement(4746..5999)
                     /gene="amiC"
                     /locus_tag="ACHXWD_000990"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR7619971.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            6231..7562
                     /gene="argA"
                     /locus_tag="ACHXWD_000991"
     CDS             6231..7562
                     /gene="argA"
                     /locus_tag="ACHXWD_000991"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR7619972.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7624..9450)
                     /gene="recD"
                     /locus_tag="ACHXWD_000992"
     CDS             complement(7624..9450)
                     /gene="recD"
                     /locus_tag="ACHXWD_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR7619973.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKRRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(9450..12992)
                     /gene="recB"
                     /locus_tag="ACHXWD_000993"
     CDS             complement(9450..12992)
                     /gene="recB"
                     /locus_tag="ACHXWD_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR7619974.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12985..15873)
                     /gene="ptrA"
                     /locus_tag="ACHXWD_000994"
     CDS             complement(12985..15873)
                     /gene="ptrA"
                     /locus_tag="ACHXWD_000994"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR7619975.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(16049..19417)
                     /gene="recC"
                     /locus_tag="ACHXWD_000995"
     CDS             complement(16049..19417)
                     /gene="recC"
                     /locus_tag="ACHXWD_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR7619976.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19430..19753)
                     /locus_tag="ACHXWD_000996"
     CDS             complement(19430..19753)
                     /locus_tag="ACHXWD_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR7619977.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19738..20145)
                     /locus_tag="ACHXWD_000997"
     CDS             complement(19738..20145)
                     /locus_tag="ACHXWD_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR7619978.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(20142..20705)
                     /locus_tag="ACHXWD_000998"
     CDS             complement(20142..20705)
                     /locus_tag="ACHXWD_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR7619979.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(20696..21166)
                     /locus_tag="ACHXWD_000999"
     CDS             complement(20696..21166)
                     /locus_tag="ACHXWD_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR7619980.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(21351..22145)
                     /gene="thyA"
                     /locus_tag="ACHXWD_001000"
     CDS             complement(21351..22145)
                     /gene="thyA"
                     /locus_tag="ACHXWD_001000"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR7619981.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(22152..23027)
                     /gene="lgt"
                     /locus_tag="ACHXWD_001001"
     CDS             complement(22152..23027)
                     /gene="lgt"
                     /locus_tag="ACHXWD_001001"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR7619982.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(23178..25424)
                     /gene="ptsP"
                     /locus_tag="ACHXWD_001002"
     CDS             complement(23178..25424)
                     /gene="ptsP"
                     /locus_tag="ACHXWD_001002"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR7619983.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(25437..25967)
                     /gene="rppH"
                     /locus_tag="ACHXWD_001003"
     CDS             complement(25437..25967)
                     /gene="rppH"
                     /locus_tag="ACHXWD_001003"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR7619984.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(26320..26466)
                     /gene="ygdT"
                     /locus_tag="ACHXWD_001004"
     CDS             complement(26320..26466)
                     /gene="ygdT"
                     /locus_tag="ACHXWD_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR7619985.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            26652..27341
                     /gene="mutH"
                     /locus_tag="ACHXWD_001005"
     CDS             26652..27341
                     /gene="mutH"
                     /locus_tag="ACHXWD_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR7619986.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            27410..28123
                     /locus_tag="ACHXWD_001006"
     CDS             27410..28123
                     /locus_tag="ACHXWD_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR7619987.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            28261..28479
                     /gene="ygdR"
                     /locus_tag="ACHXWD_001007"
     CDS             28261..28479
                     /gene="ygdR"
                     /locus_tag="ACHXWD_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR7619988.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         6811 a         7789 c         7143 g         6777 t
ORIGIN      
        1 tttttaatct gagccattat aaattgtccg ttgagcttct accagcaaat acctatagtg
       61 gcggcacttc ctgagccgga acgaaaagtt ttatcggaat gcgtgttctg gtgaactttt
      121 ggcttacggt tgtgatgttg tgttgttgtg tttgcaattg gtctgcgatt cagaccatgg
      181 tagcaaagct accttttttc acttcctgta catttaccct gtctgtccat agtgattaat
      241 gtagcaccgc ctaattgcgg tgcttttttt taccttgcga tcgcgaatta ctgatccagt
      301 tcgaccatct ctttcacgtc agtacggttg atctgctgtt tgttgccatt agcgtcttta
      361 tacgaaatca taccggtatc gttatcagtc tgtggtttgc cgtcagagac gatggtacgt
      421 ccgtcattgg tgtgcatcac atagttcgaa ccggaacagg cgctcagggc aaaagtcagc
      481 atacaggcag aaataattgc ggcagtcttt ttcatcatct tctccttaaa gcgattaata
      541 aatcttaata gtattgatta acaggctaaa attaacgcct aacactattc agcatatgtt
      601 acttacgcgg cttcgccagg atatccagat aattctgatg gttagcactc tccttgtatc
      661 aaagtgaatt ttgcgtcacg atcggtgcat caagccgagg agtaccatga acgtttttaa
      721 tcccgcgcag tttcgcgccc agtttcccgc actacaggat gcgggcgtct atctcgatag
      781 cgccgcgacc gcgcttaaac ctgaagccgt ggttgaagcc actcgacagt tttacagcct
      841 gagcgccgga aacgtccatc gcagccagtt tgccgaagcc caacgcctga ccgcgcgtta
      901 tgaagctgca cgagagaaag tggcgcaatt actgaatgca ccggatgata aaactatcgt
      961 ctggacgcgc ggcaccactg aatccatcaa catggtggca caatgctatg cgcgtccgcg
     1021 tctgcaaccg ggcgatgaga ttattgtcag cgtggcagaa caccacgcta acctcgtccc
     1081 ctggctgatg gtcgcccaac aaactggagc caaagtggtg aaattgccgc ttaatgcgca
     1141 gcgactgccg gatgtcgatt tgttgccaga actgattact ccccgtagtc ggattctggc
     1201 gttgggtcag atgtcgaacg ttactggcgg ttgcccggat ctggcgcgag cgattacctt
     1261 tgctcattca gccgggatgg tggtgatggt tgatggtgct cagggggcgg tgcatttccc
     1321 cgcagatgtt cagcaactag atatcgattt ctatgctttc tctggtcaca aactgtatgg
     1381 cccgacgggt atcggcgtgc tgtatggcaa atcagaactg ctggaagcga tgtcgccctg
     1441 gctgggtggc ggcaaaatga ttcacgaagt gagttttgac ggcttcacga ctcaatctgc
     1501 gccgtggaaa ctggaagcag gaacgccaaa tgtggctggt gtcataggat taagcgcggc
     1561 gctggaatgg ctggcagatt acgatatcaa ccaggcagaa agttggagtc gcagcctggc
     1621 gacgctggcg gaagatgcgc tggcaaaacg tcctggcttc cgctcattcc gctgccagga
     1681 ttccagcctg ctggcctttg attttgctgg cgttcatcac agcgatatgg tgacgctgct
     1741 ggcggagtac ggtattgccc tgcgggccgg gcaacattgc gctcagccgc tactggcaga
     1801 attaggcgta accggcacac tgcgcgcctc ttttgcgcca tataatacaa agagtgatgt
     1861 ggatgcgctg gtgaatgccg ttgaccgcgc gctggaatta ttggtggatt aatgacgaac
     1921 ccgcaattcg ccggacatcc gttcggcaca accgtaaccg cagaaacgtt acgcaatacc
     1981 ttcgcaccgt tgtcgcaatg ggaagataaa tatcgccagt tgatcatgct ggggaaacag
     2041 cttccggctt tgccagacga gttaaaagcg caggctaaag agattgccgg atgcgaaaac
     2101 cgcgtctggc tgggatatac cgtgactgaa aacggtaaaa tgcacttctt tggcgatagc
     2161 gaagggcgca ttgtgcgcgg cctgctggcg gtgctgttga ctgccgttga ggggaaaacc
     2221 gccgccgaat tgcaggcaca gtcgccgctg gcattgtttg atgagctggg attgcgtgcg
     2281 caacttagcg cctcacgcag ccaggggtta aatgcgttaa gcgaggcgat tatcgctgcg
     2341 acgaagcagg tttaagcata gaaccgggtc ggataagacg ttcgcgtctc atccgacctg
     2401 attgtttaac cctgacgcgc cgctttcgcc atcatcttct tcagcgcatg agaaaccgca
     2461 acaaacccaa aggtggcggt caccatcgtt gccgcgccaa atccagatgc acaatccatc
     2521 cgcttcggcc cttctgccgt ggctttcatc gcacacacag taccgtctga ctgcgggtac
     2581 accagcgctt cagtagaaaa cacgcaatcg acgccgagct tacctttact gtttttcact
     2641 acgccaaaat cgcttttcag gcgctcgcgc aacttcgccg ccagcggatc ctgaatcgtt
     2701 ttcgccagat cggtaacctg aatctgcgtc gggtcaatct gcccacccgc gccaccggtc
     2761 gtgaccagcg ggattttatt gcgccgacaa taagcaatca gcgccgcttt gggccgtaca
     2821 ctatcaatgg catcaatcac gtacgaataa cccacgctca tatactgcgc tacgttatcc
     2881 ggcgtcacga aatcatccac caccgttaca cggcactccg ggttaatctg gcgaatacgc
     2941 tccgccatga cttccgcttt tgccagcccg acgttatcgc gcagagcgtg aatttgccga
     3001 ttggtattgg tgacgcacac atcatccata tcgataagcg tgattgcgcc aatccccgtg
     3061 cgcgccagcg cttccgccgc ccaggaaccg acaccaccga taccaaccac acaaatatgc
     3121 gcgtcagcaa acagttgcaa cgctttttca ccatacagac gcgctgtgcc accaaaacgc
     3181 tgacgccatg catcactaat taccacagac atagcacctc agaattaaaa agggtgaggt
     3241 tatccctcac cctgtcgtat ccgtaaaaac ggtatacaga ataacacatc agccgctaaa
     3301 gacgttacct gcgcccgggg cggttttcag cacccagaca cgtccatagt ggttgtacca
     3361 acctgcgcgg tgtccggctt ccggcccgat cccttgatag atatcgaagt gttggccttt
     3421 gattgcacca ccgacatcca gcgccaccat cagacgcagt tcgtactgac cattaaattt
     3481 gccgttatta tccagcaacg gcacttctgc cagcaaggta gtacctggcg gaataatgga
     3541 acgatcagag gcaactgacg cgcgaccaac cagcggcacc gcacttgccc ctttcaccgg
     3601 tgcaaaagat tgcggtttaa agaagacgaa agacgggttc tgttccagca gctcgcgaac
     3661 ctcggcttca ctgtgtgttt cgccccagtg acgaatcgcc tgcatcgaca tatcttcttt
     3721 tttcacttcg ccacggtcga tcagcacctt accaatgctg cgataggcat gaccgttttt
     3781 ccctgcatag ctgaaaaagt taagcggact gccatcacca aagtcgatat acccactacc
     3841 ctgcacatcc ataatgaagt tatccatcag ggagttactg taagcgagaa tatatttatc
     3901 actcaatgcc cccgcgtaga tctccgcacg agacggcaga cgaccacgtt ttggcggcat
     3961 acggtaaata ggatactgga actcgccctg gcgggtatgg cgcgcctgaa ttaccggcgt
     4021 gtaatagcct gtaaactgca cgttaccata gttgtcggca ccttccatct gccaggcatc
     4081 aatgccgaac tggcgcatat tgcgggtatc accgcctgcg cgcagccact cttgcaccgc
     4141 gttataaaca ttactctggt tgccatacag acgcggtgac gaattacgga tatggttaat
     4201 ttgctcggca aaatcaccgg cgttaatcgg cgcgccaacg gcatctggct ggttcaccag
     4261 agagaaaggc tgggtaaatt tcccgtcttt atattgctgt ccgcgatcgg ttggtttgga
     4321 agagcaggcg gcaagcattg ccacaaccgt gcccataaga aggtacttta cccaacgtcc
     4381 tttcattgtt ctcttcttca ggttaaaaaa taaggcgcaa cgaagataac aaaccgccgc
     4441 gtttaatgaa atgagcagcc tctccctgac gcaaattttg cacaaaaaat aggctttagt
     4501 gatttgtttt tgttcaaaat catgccagat ccgtgatcgg ggtaaaaaaa aggttgcatg
     4561 aaaacgcgag cggagtatag tgcgcatcca cggaggcggg gtggagcagc ctggtagctc
     4621 gtcgggctca taacccgaag gtcgtcggtt caaatccggc ccccgcaacc aattattgaa
     4681 caccctaacg ggtgtttttt tgtttctact ctcccataaa aaaagcgcca ttcagcgcct
     4741 ttttatcatc cccttctcgc cagcgttgcc ccatcggcaa aatacgcttt aatccccgca
     4801 agaatagact ccgcaacttc ctgctggaaa gtcgccgttt tcagtttacg ctcttcctca
     4861 acgttactga taaacgccgt ttcgaccaga atggagggaa tatctggtgc ctttagtacg
     4921 gcaaacccgg cctgttcaac ttgattttta tgcagcttgt tgattttacc gagcttattc
     4981 agcactgctt taccaaactt aaggctgtca gcaatggtca gcgactgcac catatcgaac
     5041 atggtgtggt cgacatagcg gtcaccgctt ttgctcacgc caccaatcaa gtccgaggcg
     5101 ttctgggttt gtgccagata ttttgccgca gtactggttg cacctttggt tgagagcgca
     5161 aacaccgagg aaccgctcgg ctggcgactg gtaaaggcgt cggcatggat agagacaaac
     5221 aggtcagcac gctgtttctg ggcttttgct acgcgcactt gcaacggaat gaagatgtct
     5281 tcattgcgcg tcatgtacac cttcatattg ccctctttct cgatcagaga gcgcaaacgg
     5341 cgagctattt gcaataccac atctttttcg cgcgttttgt atttccccac cgcaccggag
     5401 tcttcgccac cgtggccagg gtcaagcata atgacaatcg gacgatcgcg ccctgcttta
     5461 cctggttgtg gaccactttg cgctggcggc acctgctttt cgaggtcgcc tttgttgtaa
     5521 tcctccagca gcgccagcag cgggtcctgc atatcctgtg cattggccgg atagaggtcc
     5581 atcaccagac gctctttaaa cccggcgacc ggcgcaaggg caaacagctg cggttttacg
     5641 ttttgcttta attcaaaaac catccgtacg gtttgcgggt caaattgccc gacgcgcgcc
     5701 gacttgatga acgggtcgtc tgcacgaatt tgtgccgcca tccccttgag taccgagttc
     5761 aggtttacat cttcgatatc caccaccacg cgttcagggt tactcaacgc gaactgctta
     5821 tatttcagct gacgatttga ttctaccgtc acgcgggtgt agctggacgc aggccagacg
     5881 cgcaccgcca cgacctggct aaccgcagcc aggctgacct gacttacgct caatagccac
     5941 atagcgcccg cgccttgcag taaacgacga cggctgattg cagtgttgga tcctgacatg
     6001 cctctcccga gcaaaagaaa tctaagctgt gtaacaagta aacgactaat ttgaccggaa
     6061 actttagcga aagacgcata atctgtcatc taataaacgg taaacattct ttttatattc
     6121 acggcattac tgataaaaaa gtcgctctcg cataaaattt acacttgcac cctgcgaaaa
     6181 aacagaataa aaatacaata atttcgaata atcatgcaaa ggggtgtgcc gtggtaaagg
     6241 aacgtaaaac cgagctggtc gagggattcc gccattcggt tccctatatc aatacccacc
     6301 ggggaaaaac gtttgtcatc atgctcggcg gtgaagccat tgagcatgag aatttctcca
     6361 gtatcgttaa tgatatcggg ttgttgcaca gcctcggcat ccgtctggtg gtggtctatg
     6421 gcgcacgtcc gcagatcgac gcaaatctgg ctgcgcatca ccacgaaccg ctgtatcaca
     6481 agaatatacg tgtgaccgac gccaaaacac tggaactggt gaagcaggct gcgggaacat
     6541 tgcaactgga tattactgct cgcctgtcga tgagtctcaa taacacgccg ctgcagggcg
     6601 cgcatatcaa cgtcgtcagt ggcaatttta ttattgccca gccgctgggc gtcgatgacg
     6661 gcgtggatta ctgccatagc gggcgtatcc ggcggattga tgaagacgcg atccgtcgtc
     6721 aactggacag cggtgcaata gtgctaatgg ggccggtcgc tgtttcagtc actggcgaga
     6781 gctttaatct gacctcggaa gagattgcca ctcaactggc catcaaactg aaagctgaga
     6841 agatgattgg tttttgctct tcacagggcg tcactaatga cgacggtgat attgtctcag
     6901 aacttttccc taacgaagcg caagcgcggg tagaagccca ggaagagaaa ggcgattaca
     6961 actccggtac ggtgcgcttt ttgcgtggcg cagtgaaagc ctgccgcagc ggcgtgcgtc
     7021 gctgtcattt aatcagttat caggaagatg gcgcgctgtt gcaagagttg ttctcacgcg
     7081 acggtatcgg tacgcagatt gtgatggaaa gcgccgagca gattcgtcgc gcaacaatca
     7141 acgatattgg cggtattctg gagttgattc gcccgctgga gcagcaaggt attctggtgc
     7201 gccgttctcg cgagcagctg gagatggaaa tcgacaaatt caccattatt cagctcgata
     7261 acacgactat tgcctgcgcc gcgctctatc cgttcccgga agagaagatt ggggaaatgg
     7321 cctgtgtggc agttcacccg gattaccgca gttcatcacg gggcgaggtt ctgctggaac
     7381 gcattgccgc tcaggcgaag cagagcggct taagcaaatt gtttgtgctg accacgcgca
     7441 gtattcactg gttccaggaa cgtggattta ccccggtgga tattgattta ctgcccgaga
     7501 gcaaaaagca gttgtacaac taccagcgta aatccaaagt gttgatggcg gatttagggt
     7561 aagcgatgaa aatcgccgga tgcggcaggc gtaacactcg tgcgtcgcat ccggcaatta
     7621 cgtttatccc cgcgaactaa acaacgccgc cagaccactg cgccgctcag tacgagtggc
     7681 gattgccgca cttaatatac gctcatcggc atacagcgac agacggcgac gcgcgcgggt
     7741 caccgcggtg tataccagct ctcgcgttac tatcggcgtg cgttggctcg gtaaaatcaa
     7801 cgccgcatgg tcgaactccg atccctgcga tttatgtacc gtcatcgccc acgtagtttc
     7861 gtgctctggc aagcgactcg gttgcacaga cttaatattg ccgtccggca tcgcaaacca
     7921 gacgcgcgtc ccctgcccgc gatccagcgc aataccgata tcgccattaa acaacccaag
     7981 cgcgctgtca ttacgggcaa tcatcaccgg tcgaccttcg taccaacgag agtgcgaatg
     8041 acgatgaatt ttgcgcttct gttgcataaa ctgctcaatt cgctcattca gtccagccac
     8101 gccaaatggc ccttcccgca gggcgcacaa aagttggtac tcattgaacg cctgaatgat
     8161 taaatccggc tcggcacgcg cttgcagcag atccagataa cgtccataac ccgcaagagc
     8221 ttcctcaagc atcgcaatat aatcttcgcc gctctgtaaa agccgttttt caatatcagt
     8281 aaaatcctgc tgaaaaacgg ttttcactgc cgttttatca ccacggttga tcgccgcagc
     8341 taactgacca atgccagaat cgctgccgaa acgatagctt ttttgcagca ggcagagact
     8401 gtcgcgcaaa gatgccgctt ctgtgccggt tcctgccgga acgtgagttc ccgtcaggcg
     8461 gcttagctgc ccggcacgct cggcggtaaa gccggcattg gcataagcgc agatatcgcc
     8521 cagcacagcc ccagcctcaa ccgaggccag ttgatcacga tcgccgagaa agatcactcg
     8581 cgcatgatcg ggcaaggcgt cgatcagtct cgacatcata ggcagatcga tcattgacgc
     8641 ttcatctacc accagcacat caagatgcag cgggttaccg gcatgatgac gtaaacgctg
     8701 gctacccggc tgcgcgccca gcaatcggtg caaagtgctg gcatcttccg gaatgcgtct
     8761 cttttgttca tcggtcagcg gtaactgtcg caaagccttg ccgagagatt cggttaagcg
     8821 cgcggcagct ttacccgttg gtgcagccag acggatacgg cagcgttcgc cgtcggccat
     8881 ttgaattaac gctgccagca acttcgccac ggtggtcgtt ttaccggtgc ctgggccgcc
     8941 ggaaatcacc gagatccgcc gcgtcagcgc cactgctgcc gccacttttt gccagttaat
     9001 ttcatcgctt actggaaaaa gtttgtccag ggtttgcgcc agtagagctt catcaacctc
     9061 aatggcatga ttcacctcat tgaagaagcg cgctaccgtc cgctcgttac accacatgcg
     9121 attcaaataa agacgatcgc cacagaggat catcggcgtg ggttcatctc ccctgctgac
     9181 cgcttgagaa gccagcaagg attcttccca attttgtagc tcaccgattt cactgacaca
     9241 ggtcgccaac agcggatgcg acgcctcgtt attttccagt cgtgaaagcg gcaaacagac
     9301 gtgtccctct ccggcatcat gacttaacag tgccgccgcg agggtgacgg caggatgttc
     9361 atctcccgcc acggtcaggg caaattgcac atccagcggg cgtagctgtt tgtgctccac
     9421 agcttccagt aattgctttt gcaatttcat tacgcctcct ccagggtcat accggcaaac
     9481 atctcatcca tcagggcaat caacccggcg ttgggtcggg ttgtgtaaat cccttgttgc
     9541 ggatgttctt tatcaacgcc acgcaggaac agatagataa cgccgccaaa gtgacgatca
     9601 tagtcgtaat cagcaatgcg atggcgcaga tagcgatgca gcgccagggt ataaagctga
     9661 tattgcagat catagcggtg tgcctgcatc gctgctgcca tagcctgttg ggtgtaagcc
     9721 gaactgtctt cacccagcca gttggattta tagtcgagca ggtaataacg cccttcgtgg
     9781 cggaatacca ggtcgataaa gccttttaac atgccgcgta cctgcatgaa ctccagcggc
     9841 gggcagccag cggatagcgg gtcaaactgg cgaattagtg catcaagctg gctggcgatg
     9901 agcggttcgc taatcggcag gtaaaactcc atctccacct gtttatcgcg gtcggaaagc
     9961 tgactcaggc taacacccgt ttcattgaga ggtgcctgga ggacagccgt gatccactcg
    10021 gtcaacaccg gttcccactg cgattcaaag ccgccgagtt ccagtttttc ctgtacccag
    10081 ttcgggtcaa ccggctgggt aaaatcgagg tcttcaaaca aactgtgcaa gaatgtcccc
    10141 ggtgacgcac cgcgcgggaa ctgatgcggt gttaacgtcg gttcttcaac gacgctgacc
    10201 acgcctgcgg catcaacatc cagccgtggc atcagatcct gagcgatacc gtgaccacgc
    10261 tgctgcaagc cggagtagct ggtgacacgc cagttatcgc cgggcaatcg ttgtagtgtc
    10321 ctcgcattca gttctgcagt taaagcatca ttaacctgcc agggctggtt atcaccagtt
    10381 tgtgccgttt gccaggcaat atcatcatcg cataacgctt caatacaggt gcgaagccct
    10441 gccgcatctt gcggttcccc tttttgcagc aaacgcccga gcgcactttg gtggacgtcg
    10501 gtgtcacctt ttttatcgcc acgacggcgc accagcggtg caacgccgag actgcaatgc
    10561 caaaccgaac gcgtcagcgc cacgtaaagc aaacgcagat cttccgccag acgttcgacc
    10621 tccgcgaggt cgacgctttc tggcgcagca ttaagatcca aaactgcctc aaacgagtgg
    10681 cgatcgtgat aaaacgcctg atcctggacg cggaaattgg taataaatgg caaccagacc
    10741 agcggatatt ccagcccttt cgatttatgg atcgtgacaa tctgcaccag atgtttatca
    10801 ctttcgaggc gcatttgttg gctggaggca ttactgtctg gctcgaggat atgttgcgat
    10861 aaccagcgta ccagcgcatg ttcactttcc agctgcgttc cggcttcttg aagcagttcg
    10921 ctgatatgca agatatcggt aagacgccgc tcaccgcctg ccgttgccag caagttttct
    10981 gcaatgttac gcgccgacat cagcgcccgc agcatcggca taacgccacg tttgcgccag
    11041 atttgccgat aaccatcgaa ctcttcgact accgcatccc acgcatgttc gtcattattc
    11101 agcgtttcaa tatccagcgc attcagcccc atcattgacg ttgccagcgc actgcgcagg
    11161 gtgttctcac gttcgggcgt catcaccgcc tgcaacagcc aaagcatttc ctgcgcttcc
    11221 agagtttcaa aaacactatc gcggttcgaa aggtaaacgg aagggatttc cagcaacgtt
    11281 aaggcatcgc gcacctgggc ggcctcctgg cggctgcgca ccagcacgct gatgtccgaa
    11341 gcacgcaccg gacgcgcgtc gtcgccgttc atcagcaacg cttcgccccg ctgtccggct
    11401 tgtagccagt cgcggatttg cgcagcacat acctgcgcca tggtactttg atagtcgcca
    11461 acgccgcagc tttcgccttc catcagccac attttcatcg caggctgcgt ttcaccttta
    11521 aagacaaaac gtaacgcctg atttttcccg gctgatttca ctggaataaa cggtatttcg
    11581 cgaaacatga acgcgtcatc agtctggctg aaaagcttgt tcacactgtt caccattcca
    11641 ggtgcggagc gccagttggt gtctaaagtg tagtgggcgt gaacttcact acgcgccttc
    11701 atataagtga agatatccgc accccggaat gcatatatgg cctgcttcgg gtcgccaatc
    11761 agcaacaatg cggtttctgg ctgatggtgc cagatacggc gaaaaattcg gtactgctgg
    11821 gggtcggtat cctgaaattc atcgatcatt gccaccggga atcgcgtacg gatcgccgct
    11881 gccaacacct cgccgctttc gctacgcagc gcggaatcga gccgacttaa catgtcatca
    11941 aaacccaatt cgccacggcg gcgtttttca cgcgctactg tttcgcggat ctcagccaat
    12001 gcgcgggtga tcaccagatc acggatcgac aatggttctg caagcagttg atcgatcgcc
    12061 tcaaacagtg gatgtcgcgg ggtttccccc ccggctttcg tgcgatcttc taagaaacgc
    12121 tgggagaatt tttccagcga ctccggcaac tgataactgt ttgtctcttc ttctgcccag
    12181 gcgctgatct tgtcgatcca tttagcctga ttgctacggt taaacttgcg tcgatcaata
    12241 ccagaagatt cgatcagcgc atccagttca cccactgcgt cgcgccactg ctgttttacc
    12301 gtatcaatac gcgccacaat ttgcgcgtga cgggaagcta gcgtttcatc atcgggcggc
    12361 ggtgctttga taaccggcgc ttcgccttgc agataacgat taatatcgcg cagcaacgcc
    12421 tgcggccctt tccaggtttc aaagacgacc tgggctattt cacgcggcag cgggtagcag
    12481 tggcgacgcc agaaatcggc gcaggcctgg tagcgtagca gagactcatc ttcaatcagc
    12541 tgctgctcaa acagcatgcc ggattcaaag gcattcaggt tgagcatgcg ctggcaaaag
    12601 ccgtgaatag taaagactgc cgcttcatcc atctgccgtt cggctaacaa caaccactgc
    12661 gcggcttgcg ctttatcgtc gacctcttcc agcaggtgtt cgtacaatgg attgtcggtg
    12721 gtttcacgca gacaggcgat gcgcaactcg tggatattgc tacggatacg accgcgcaat
    12781 tctgccgtgg cagcctcggt aaaggtgacc accagcagtt cttcaacggt cagcgggcgg
    12841 ggaaaggcgg cggaaccgcc tagtccaagt aacaggcgca aataaagcgc cgcaatcgta
    12901 aaggttttgc ctgtgccggc agaggcttca atcaggcgct cgccctgtaa gggcaagcgc
    12961 aaaggatcta gtgtctcggc gacatcactc attcttttca ctcatcaggg gcattgtttg
    13021 ctgcaacgcg ctgacgttct cccacacttt ccagccttca ggatgcacat attcggcttt
    13081 cccgttttgg ctgccggaaa tctgcgacag gatagccatg ccttgcggct cgactaccgc
    13141 ctgatggaag aaatcggcca gtttttgcgg cgtcagcagt ttaatctggg ccacgatttt
    13201 atcacgcgaa tcgaagcgca tattgccgcg atcgaaatct ttacttaact tcgatgcttc
    13261 ttcgccgagc gtttgcggtg cctgcagcat ctgggtaatt accgcctgct ggatttgcgc
    13321 aaactcatct ggcttcatcg ctcgcaattt tgcctctgcg gttgggaaaa acgccttgta
    13381 acgctcccac aagaatgaag gctgtttatc attgctttgc aaaaggaagc ccatgcccca
    13441 ctgacgcccc acgctcattg gaaacgcaaa cacggcatag cccaattgtt cttcggtacg
    13501 caactgattg tagaaccacg gctgtacgat ctgccccaac agagagctat aggctgagct
    13561 ggtgtattca tcgtagccag tcggtacaaa taccgctgcc agtgcggagt cggtgctgtt
    13621 accggctttt tcaaagatga cggattgttt tttatcgacc actacatctt tgtttcgaca
    13681 ccactctgaa ccatcagcgc ccaactgttt ttgcacatcg cgtgccagcg ttgttgcctg
    13741 ggcctcggtc atgttgccga taaccataaa ctctggtcga gcccctgatt ttaaggcgtc
    13801 gcgataagcc agcacctctt tcaacgtaat ggagggcaaa attttacgcc gttcatctcg
    13861 cgagaagtac ggcacttgcg agagcatctg cgcgggcata atcgcctgct caaacgcttt
    13921 acccttttct gcggaatcca tcatctggtt ataccaggac ttcgcctgct caagctgatc
    13981 ttccgtagcg gtatagctaa agtacccctc gagcaatgcc tggaacagct gcggcagacg
    14041 ctgggtgtaa ccattagcat taaccataag gccgttgtta gcgttggtgg aaaaacttat
    14101 gccaccaacc gacgcctggt tgcttaactg atcaagcgcc agccctgcga gataatcatt
    14161 gagcgcaaac atcacctgat tgcgggcgct gtccatggct ttcggattac gcaaaatcag
    14221 gctgacatca gctttgggtt cgctggaaaa ataacggctt ggcgcataca ccacgcgcag
    14281 attcgactca tcaacaatca gctctggatg gtcatatttc ttctctgact taatcagcga
    14341 gaaatcgtca ggaatatagg ggttaagctc cggtaaggag agcgcaatat tggcagcttt
    14401 ttgctgccag tcagcgaaag tttgttcgct gattttatcg acctgatacg gcgcatcgac
    14461 aaaataagcc gttttattgt gcggctcttt cgggctgata taccagatac gcgcattctg
    14521 cggcgtcatc atcgccagac gttcttttac tgctttagca tcgtaccgat cggcaatatt
    14581 gactgcatcc agcgtatgct caaccggaac gcgaatcatg gtatctgcca gccattcgac
    14641 gtaatccata tcacgggtga tcgacggata acggaagtcg atatccagca cattcgccag
    14701 ttcatcgaag tattgtttat caatgccttt ttcacgtaac agattgagat agctaaaaat
    14761 tgccgccaca acctgatcgc gattagccag gcctttatcg gttaaagacg cagagatcgc
    14821 taatacgccg ctgttgccgt tgacgatagg atcggagttg gcgctaatgc cctcaactaa
    14881 tccctgcttt tgcagccagt cggaaagtgt acctgggctg cgattaccaa tcagataggt
    14941 aatcaattca tccgttttac tacggaactt cgctgagttg ttatcgatgc gaaactcaac
    15001 gcgcagaact ttacgcggca ttgccgggac gtaatgaatg ataatgccct tttgcgcgtc
    15061 ggtgactacc ggcacggtga tttccggttt tttgctctct ttgttcggca cgcgaccaaa
    15121 ggtgtccgcc gccatttttg ccaactccgg cagcggttta ttgctgtaaa taaccgcctt
    15181 catcagattg gcggaatagt acttctcgtg gaaatctttc agcgcctgct gcaccggatt
    15241 accaggttta tcgcttaaag tttcgaggtt accaccagaa aactttgaac cggggtgtgc
    15301 cgggttaatg gtttctgcgc tgacctgtgc catgcgcatc ccgtcacgcg tacgcgccat
    15361 ggttaattca gcgttcaccg cattacgctc acgttcggca tatttcttgt cgagcaaagg
    15421 ttcagcaata gcatcggcca ggcggtctac cgcaccaggc aaggcgtcgt tctcaacttc
    15481 cagatagaaa gccgtgcgat acggcgcagt gctggcattg tgactaccgc cgtgcatttt
    15541 gagatattcg gccagactgt cagcctgcgg gtactttttc gaccccatca gactcatatg
    15601 ttcaaggtaa tgtgccagcc cctggtacgc ctcgggatct tccagcgacc caacgggcac
    15661 caccagcgcc gagagcgatt taactgcctg cggatcagaa accagcaaga ccaccatacc
    15721 gttatccaga cgtatagcct gatactggcg gttatcttta tcacttttac ggatggtttc
    15781 ctgaatcggc tgccatcccg tttctgcctg acttaagggt gcccaaaggg caactaacaa
    15841 caataatgct ttgaaccagg tgctgcgggg cattctcgga cctcataatc aacttaattt
    15901 tctgtccaga ttcaacacgt taacgcaaat catctgccag aatttaatct tgtgctgcac
    15961 gagtcagcct atgtttatat aaccatcagt ccgtgactgg tgcgcatcat aaagtaagcg
    16021 gatagattgc gcaattttta tacagcactc atgactgatt aaagcgaaac agcggtaaca
    16081 ggaaacgttg cgactgttca acgatggcct ccattgtctc tggtgttaat tgccgccaga
    16141 gcctttgata ccagatatca tcaccttcgc cacgcaccat catgttacct tcgtaagcct
    16201 gaaggaattt cgtacgggct ttttgcaacg tggaatcgtc atccagcatg gcatcgtttt
    16261 gcgcgtcata acaggttttt agccacgcgc cgccactttc aggtaacacc agcaatggcg
    16321 cggacatccc ttcacgatac ccctcaatca gttgtgagag gtaatgcaaa gcctgttcgg
    16381 ctgcaagcgg cggaaaacgc cactcgccgt ctttgcgtag aaaaaggcga ctttcaccat
    16441 taccaccgct ggcacagtag acaaggtgtt ccagccaaag ttgcattccc tgcgccacac
    16501 ttaataaaga gggacgccag cgcaacaggc catccggctg cacctgcggc aaccagccag
    16561 ttatctgcac accgttgcag gcgagatcaa tttccatact ctgccccggc tggcgacagg
    16621 caatgactct gtcggcaagc tgctgcatct cctggcactg agtttcccag aaaatttcac
    16681 caaaagcgcc atacggtaaa tcccctgccg cccggaagcg acggaacaag cgttcggcat
    16741 catcctgctc aaccagtgca ttcaataact gctgattgat ttgataacgg ctaagccctt
    16801 ccagaataaa tggctcggtg tcggggattt cgctgtcttc agtacggaag ttcacctgca
    16861 aacgcatctg gaagaacgcc cgcaccggat gtgcccagaa tcgttgtagc gtttccagcg
    16921 gcacggtttc cggtaaggta aacggcagcg gctgaacaaa ttcagaatgt gctttaccag
    16981 cctggctggc cgcaggtagc cattcacgag catagctttg tcgttcgcct ggctgatagt
    17041 tttgtggatc aaacggcatt cgggtatgga ggcaagtaag atgcgctttt acccttgcct
    17101 cgctttcatc acagttgagc gcttcatcgc ccggtagata atgactttgc ccgatgtagt
    17161 cgatcagttc ctgcaccagt accgacggga aacgttcact gttatcctga atggaacgcc
    17221 cgatatagct gatatagagt ttttgctgcg cggaaattaa cgcttccagg aacagatagc
    17281 ggtcgtcatc gcgacggcta cggtcgccac gcttcggttt ctggctcatc aggtcaaagc
    17341 ccaatggcgc aagctgacgt ggataaacgc cgtcgttcat tcccagcagg caaaccactt
    17401 tgaacggaat tgaacgcatt ggcatcagag tacaaatgtt aaccggtccg gcgagaaaac
    17461 gctggctgat acgttcttga tccagacgct gtgccagttc atcacgcaat agtgacagcg
    17521 gcaccgcgtc gccatactgc gcacctaaac cttcggcgat aatcgcctgc cattgttgtt
    17581 cgatcagcgt catcgccgct tcggtttccg catccggcag gaagaaggcg ctgagcatat
    17641 cgcgacaaac cggcaaccac tcttctagcg gacgctcctg tgccagcccg cgacgccaga
    17701 tgtttagctg cattagcagt gaagccagat gccccaccag ttctgcaatt aagccgctcg
    17761 attcatcata aggtagaacc gattgccact cgccctgcgc gctctccatc gcgtagccca
    17821 gcaacatacg cgtcaggcca aatcgccagg tgtgttgtcc ggtggcgggg agttccagct
    17881 cgcgaacgtt gtcgtcatct atcccccaac gaatgccgga ttcgttgacc cactggcgta
    17941 aataacgcag cccttcttcg gtgatgtcaa accgcgccgc cagcaccggc acatccagta
    18001 atgccagcac gtcttccgac acaaagcggc tgtctggcaa cgataacagg ctgataaacg
    18061 cttccagtac cggatgcgac tgccgcgccc gacggtcgga aatggcgtaa ggcaggtaac
    18121 gatccgcagg tgcgctacca aacacagccc gaataaacgg actgtagctg tcgatatcag
    18181 ccaccatcac gatgatgtcg cgcggagtaa gtgtcgggtc ttcttccagc atcgccagca
    18241 ggcgatcgtg caaaacttca acttcacgct gcgggctatg gcaaacgtgg aaggtgatac
    18301 tgctatccag tggatcaagc gggcgtttgt tatcgctacg ggaaaactct tcgatgttca
    18361 caccagcaac ggcacggttt tccagttcca gaatgtcaga ctgaatgtta tgcagcaggt
    18421 tatctggcgt gacatcgaca aaggcgtcca gctcctggct gctctccagg tcagaaagga
    18481 gataaatgta gtcgcgccca agcttacccc atgaagccag cagcgggttg ccgacatcct
    18541 gttcaccatc gctgttaaag agctgcccgg cattttcgct atcacgaaat agcggcaatt
    18601 cgcgatcttc aaaactgtgt cgacgctgac gggtcagcag tttcgccaga taagcaggat
    18661 ctttaatatc gccccagtaa taacggcagg ggttggtaaa caggagatgg atttcaatat
    18721 gtttacccag cgcctgtagc gcctggagat aaacaggcgg taacgcggaa ataccgcata
    18781 taaagacgcg cgaaggtaac cccggcgggc aggtcgtcgc ggactccagc gtttcgataa
    18841 agcgctgata gagattggcg cggtgccagc gcggttgccc gagttgatgg gtatattcca
    18901 ccagcgcctt ccacaacggg gcttgccagg cctgtgcttc tcccagccct tcaaccaaat
    18961 gtcctgtttc ccactgtgcc agccagtccg gacgatagac cagatactgg tcaaacaggt
    19021 ccgccgcttt tgaggaaagc tggaacagtt ttcgcttgtc gctatcgtca gtcagataat
    19081 gccgcaacag ggtaaagtct tcgcgctcca gcaattgcgg cagcagagtc atcagtttcc
    19141 agctcatgct ctgtttgtta aaggcgctct ctttggggat ttccggtaac acccggacga
    19201 acatatccca gataaagctc gctggcagcg gaaaatcaat gtttgccgca atactaaact
    19261 tttgcgacag ggtcatttgc agccactgcg ccataccggt actttgcacc agaatcatct
    19321 ctggttcgaa aggatcgtcc agccgttcgc gttcgacaat aaactccatc aacgcttcca
    19381 gcacgtccag acgattggaa tggtagaccc ttaacatagc ggctcctgac tactgacgat
    19441 tcgggcaatg caggcgggtc atctcgcctt ctctgccccc gggtgaaact agcgtaacgc
    19501 tgatgctgac acatcccgcc tgcgatgtct gcattcggtt gacctgccag ttggcaggtg
    19561 gcgaaatcgc gcgcagttgc gtttgctgcc aaccatgccg ccagagctgt tggtactggt
    19621 ttctgctggc gagactgttc attaatgttc gctggatacc cgataacgca gtgacaatca
    19681 tcaccatcag caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca
    19741 gggaagctga cataacgccc tctctttcag cggacaaaaa tcgctccagc cgcgtggcga
    19801 aaaaacaatg tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc
    19861 ggcaaccaat aaggcttcat tatctgccag taaacgcaaa caaacccggg catcggttcc
    19921 agcgtactgc gagcactgaa ctgcgggctg cgtctgccag gagtgcattt tcccccacgc
    19981 cagcgccgac tgaacgatgg cctggcggcg caatgactga ctttctatgc tcacgcgaga
    20041 ggcaaaactg cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag
    20101 catcaggacc agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc
    20161 acgctatagc tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta
    20221 accgtcaaca ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata
    20281 atgatggcat ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg
    20341 cgtagcgttt ccagcacatg ctccttcaga cgaaatccaa tctggtcaga ctcttttacc
    20401 ggttcgcgat cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc
    20461 tgtccgacaa tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg
    20521 agatgcttcg cgacggtaaa tacccgcagc cagatttcat cttccagcgc cagcttacgg
    20581 gtgctcgtta aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat
    20641 acgctactga tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca
    20701 ggcatccttc tgtttctcct tgctgacaaa gccggagtct tccccacggc gaaaccacca
    20761 gccaccactc gcccgttgag tttttgaagc gaatatgccc ggcccatgcg gtattgcgca
    20821 ggccaaagaa agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca
    20881 caaagaccaa tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc
    20941 accataacgt cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat
    21001 cttcacgtaa atagagcaaa tagtcccgcg cctggctggc ggtttgccat agccgttgcg
    21061 actgctgcca gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca
    21121 tcgcgaccag cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga
    21181 gcgaggagaa aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt
    21241 tacgccgttg caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg
    21301 taaaaaaaac cgacgcacac tggcgtcggc tctggcagga tgtttcgtaa ttagatagcc
    21361 accggcgctt taatgcccgg atgtggatcg tagccttcaa tctcaaagtc ttcgaaacgg
    21421 tagtcgaaga tggattcggg tttacgtttg ataatcaact tcggcagcgg acgcggttcg
    21481 cggcttaatt gcagatgagt ttgatccata tgattgctgt acaggtgcgt atcgccaccg
    21541 gtccagacaa aatcacccac ttccagatcg cactgctgcg ccatcatatg caccagtaac
    21601 gcgtagctgg caatgttgaa cggcaggccg aggaagacgt cacaggagcg ctgataaagc
    21661 tggcaagaga gtttgccgtc tgccacatag aactggaaga atgcatggca cggtgccagc
    21721 gccattttat ccagttcgcc tacgttccac gctgaaacaa taatgcggcg ggaatccggg
    21781 tcgtttttca gctggttcag taccgtagtg atctggtcaa tatgacgacc atctggcgtt
    21841 ggccaggcgc gccactgttt accatacact ggtccgaggt cgccgttttc atcggcccat
    21901 tcgtcccaga tggtgacatt gttttcgtgt agataagcaa tgttagtgtc gccctgcaga
    21961 aaccacagca gttcgtggat gatggaacgc aggtggcaac gtttagttgt caccagcggg
    22021 aatccatcct gcaggttaaa acgcatctga tgaccaaaaa tggaaagcgt tccggttccg
    22081 gtacggtcgt ttttctgtgt gccttcgtcg agcacttttt gcatcagttc taaatactgt
    22141 ttcatggttc ctcaggaaac gtgttgctgt gggctgcgac gatatgccca gaccatcatg
    22201 atcacacccg cgacaatcat cgggatggaa agaatttgcc ccatgctgat gtactgcacc
    22261 caggcaccgg taaactgcgc gtcgggctgg cggaaaaact caacaatgat gcgaaacgcg
    22321 ccgtagccaa tcaggaacaa gccagagaca gctcccattg ggcgcggttt acgaatatac
    22381 aggttgagga taataaacag caccacacct tccagcagca gctcgtaaag ctgtgatggg
    22441 tggcgcggca gcacaccgta agtgtcgaaa atggattgcc actgcgggtt ggtttgcagc
    22501 agcaaaatat cttctgtacg ggagcctggg aacagcatgg caaacgggaa gttcgggtca
    22561 acgcggcccc acaattcacc gttaataaag ttgcccagac gcccggcacc aagaccaaac
    22621 ggaatgagtg gtgcgataaa atcagaaacc tggaagaagg aacgtttagt acggcgggca
    22681 aagataatca tcaccacgat aacgccaatc aggccgccgt ggaaagacat gccgccgtcc
    22741 cagacacgga acagatacag cggatcggcc ataaactgcg ggaaattgta gaacagaaca
    22801 taaccaatac gtcccccgag gaagacgccg aggaagcccg catagagtaa gttttcaact
    22861 tcatttttgg tccagccgct gcccggacga ttcgcccgtc gtgttgccag ccacattgca
    22921 aaaatgaaac ccaccagata catcaggccg taccagtgaa gcgccacggg tcctattgag
    22981 aaaatgaccg gatcaaactc cggaaaatgc agatagctac tggtcatctg tcaccacaag
    23041 ttcttgttat ttcgctgaaa gagaacagcg attgaaatgc gcgccgcagg tttcaggcgc
    23101 tccaaaggtg cgaataatag cacaagggga cctggctggt tgccggatac cgttaaaaga
    23161 tatgtatatg atccgcgcta taaccctccg cgaatcagcc cgcccatgcc gcgacgctcc
    23221 ataaaggctg caacctgatg gcgaacttcg gtcgccagtt gcgcttccag actacgctgc
    23281 gcaagatttt ctgcttcggc ataatcaatg cgccgcagca ggtattttac ccgcgctaca
    23341 gaacgtccgt tcatagacag atggcgatac ccaagcccaa tgaggattgc cacgcacatg
    23401 ggatcgcccg ccatttcacc gcacaaacgg agatcgattc catgtatttc cgcttcccgg
    23461 gcgatcatcg ccagagctcg taacattgca ggatgaagac tgtcataaat gttcgccacc
    23521 cgggtattgt tgcgatcaac ggccagaatg tattgagtca gatcgttggt gccaacagag
    23581 atgaaatcga cccgctttgc cagatgcggc agcataaata ccattgacgg cacttccagc
    23641 atgatgccga tacgtggttt gggaatttcg taaccgatca tctcctcgac ttcacgtccg
    23701 gcacgttcaa tcaggcggcg tgcttcatca acttcatcga ggcttgtgac catcggcaac
    23761 agaatattca ggttgcccgt agcggcatta gcacgcagca tcgcccgcac ctggatcaag
    23821 aagatctccg gctgatcgag cgtaatgcga atcccacgcc aacccaggca tggattctct
    23881 tcgctgatcg gcatgtaagg cagctgctta tctgctccga catccagcgt acgcaaggtg
    23941 acgggtttat cattgaacat ttgcagcatc ccctgatact gcgccacctg ttcttcttcc
    24001 gacgggaaac cactttgcag catgaatggg atttcagtgc gataaagacc gatgccatca
    24061 atacggctgc ccagtttttc ttcatgttcc gggcttaaac cagcattgag catgactttt
    24121 atacgctcac cgctttttaa ctgcgcgggt aaattgacgt catcttccgc cagacggcta
    24181 agctcaatct cttcactaat tagccgctga tattcttgca gcagtaccgg ctccggatcg
    24241 accagcaatt caccgcgata gccatcaacg atcagcgtcc tacgatgcag caccgaaggc
    24301 tgaatatccg cgcccatcac ggtagggatc cccagcgcac gtaccatgat cgcagcatgg
    24361 gagttggctg cgccatctcg cacgacaaca ccgactaagc gatcctgggg cagctcagca
    24421 agagttgtcg ctgacagttc atctgccacc agaatgaaac gttccggcca ggcgttcggc
    24481 ccttgattag cgtcatcaag atgaaacagc aatcgctgac ccagcgcacg taaatcgcca
    24541 gcccgctctt tgagatagtt atcgcttagc gcggcaaact gttcggcaaa tttttcaatg
    24601 accgttttta ccgcccactc tgccaccgag cctttatcaa cctcggcaaa caattcgcga
    24661 cgcagccggg tgtccgaaag taggtgcgag taaagatcga aaatagccgc cgtttctttt
    24721 tgtgctccgg cggcaaagcg tttgctgtag cggcgaaact cgtttgccgc ttcttccagc
    24781 gccccggtca gtcgttcgcg ttccagagcc ggatccagcg ttgatgcctg atacacctgt
    24841 tccattaaag gtaacgtggc atcctgccag ccttcggcaa tcgccacacc aggtgctgcc
    24901 ggtaatgcgc ggattcgcgt ctggcgatat tgcccaaaca aggcagtcaa ctgcgactga
    24961 gaaagaatag ctgccatctg ggtggcaagc gtcaccagga aggattcttc actttcgtca
    25021 tactggcgca actctcgttg ctgtaccacc agtacaccaa gcaactggcg acgttgaata
    25081 attggtacgc ctaaaaacgc gcggaaacgt tcttctttta cggaggggat gtatttgaag
    25141 ctggggtgct tttgcgcatc tgcaaggttt atcggttccg ccagcctgcc aaccaggccg
    25201 acgatccctt catcaaacgc gagcgttaca gtgcgaccgc gtggtttttt cagcccccgg
    25261 gtcgccatca ggtagtaaca acgtcgatca tgatcggcca ggtagaccga acagacctcg
    25321 gtatccatcg caagacagat gtcggtaacc agaatattta acgcctcatt caggcgtggt
    25381 gcgctggcta ccttttcgac tatttcgcgc aggcgagtga gcataattgg cgtgacttaa
    25441 cctcttttac gtcgataagc agatgtgttt tgtggttttg gcgtattttc ctgcagtgac
    25501 atcaccacac tcgcgaactc tttcattacc ctacggtaga catcacgttt aaatgacacc
    25561 acctgtctga ccggatacca gtaacttacc catcgccagc cgtcaaactc tggtgtactg
    25621 ctggtttgca tattgatttc tgcatcgccg ctcaccagct gcaagagaaa ccatttttgt
    25681 ttttggccga tacaaaccgg cttcgtgtcc caacgcacca aacgtttcgg taatttgtag
    25741 cgcaaccagt tacgcgttga agcaaggatt cgaacgtctt tgcggcttaa tcctacttct
    25801 tcaaacaatt cacggtacat cgcctgctct gcggattctc cggggttgat tccgccttgc
    25861 ggaaattgcc aggagtgctg accaaatcgc cgggcccaca ttacctgccc ctggcgatta
    25921 caaatcacga tacctacgtt tgggcggtag ccatcgtcat caatcaccgg actacctcaa
    25981 aataaagctt tatatacgaa tgattgtttc atactccaag aagacggtaa accactctct
    26041 gcagggcatt acacactaat aacaattgaa taactcacag ttatgtgcag agttataaac
    26101 agaggaaggg gtggatagcc gtttttattc accttttctg tggatagagt tgtgaagaac
    26161 tacggaatta ctacgggaaa acccggagca ttctgaataa gcccgatata gacattttaa
    26221 aatattcatt attagtcaat gcattacatg taatttgagt aatgaaaaag agtaattcgt
    26281 gacccaggtc acacctctca tttacgggtt ggcgaaagat caaccaatgc cgtatttatc
    26341 cacagaatgt gccactaagt taagcactga accactaaaa actggagttt cgtcgcacgt
    26401 caaggctgta aatggaaaca gtagtggagg tttttcacag ttatcccagc tttctgtgga
    26461 taacatggtg taagatcctg tttattttca gtgaccagat ttggaaaacc tattgcagtg
    26521 ttgcgcaact cgattaccgg cagcctaaaa agcaatataa atcagagaat tgaacaacac
    26581 atgtggaaaa agttacactg cgaatattcg gcacataatt gctgtttgtt ttttaaccaa
    26641 ggtatcatga catgtcccaa cctcgcccac tcctctctcc tcccgaaact gaagaacagt
    26701 tgttagcgca agcacagcaa ctttctggtt atacattggg agaactggcg gcacttgccg
    26761 ggctggttac gccagagaat ttaaaacgcg ataagggctg gattggcgtg ttactggaga
    26821 tctggctagg tgccagcgca gggagtaaac ctgagcaaga ttttgctgct ctgggcgtgg
    26881 aacttaaaac tatccctgtg gatagtcttg gtcgtccgct ggaaacaaca ttcgtttgtg
    26941 ttgccccgtt aacgggtaat agcggggtga cctgggaaac cagccatgtg cgccacaagc
    27001 tcaaacgcgt actgtggata ccggttgaag gtgagcgcag catcccgctg gcgcagcgtc
    27061 gcgtaggatc accgttgctg tggagcccga atgaagagga agaccgacag ctacgcgaag
    27121 actgggaaga attaatggat atgattgttc tcggtcaggt tgagcggatc accgctcgtc
    27181 acggggagta tttacagata cgaccgaaag cagcgaatgc gaaagcgctt accgaagcca
    27241 ttggtgcccg gggcgaacgg attctgacgc tgccacgcgg cttttatttg aagaagaatt
    27301 tcaccagtgc actactggcc cgtcattttc tgatccagta gccatcgctt tgacctgccg
    27361 ctttccgggc atataattac cgcttcattt ttttggcagg gctttttaga tgttatttgc
    27421 atggataacc gatcctaacg cctggcttgc gctcggtacg ctgacgctgc tggagatcgt
    27481 tcttgggatc gacaatatta ttttcctttc tctggtggtg gcaaagctcc ccacagcaca
    27541 acgtgctcat gcgcgccgtc tggggttggc gggagccatg gttatgcgtc tggcgctgct
    27601 ggcatcaatc gcctgggtta cgcgcctgac aaatccgctt tttacaatat tcagtcagga
    27661 aatttccgcc cgtgatttga ttctgcttct gggtggcttg ttccttatct ggaaagccag
    27721 caaggaaatc cacgaatcca ttgaaggtga agaagaaggg ctgaaaacac gcgtttcatc
    27781 attcctcggc gctatcgtgc agattatgct gctggatatt atcttcagcc tcgactcggt
    27841 gattaccgct gtgggtctgt cagatcacct gtttattatg atggccgccg tggtgattgc
    27901 cgtaggcgtg atgatgttcg ccgcgcgctc gattggtgat tttgtcgaac gccatccttc
    27961 ggtaaaaatg ctggcgctct ctttcctgat tctggtcggc tttaccctga ttctggaaag
    28021 tttcgacatc cacgtaccga aaggttacat ctacttcgcg atgttcttct ctattgcggt
    28081 tgaaagcctc aacctgattc gcaacaaaaa gaacccgctc tgatactccg tacgctctcc
    28141 tgcgggaggg cgtactcact cctgttaccc tcacagttta agattttcct gctttcaaga
    28201 tatataacgt cggtttataa acagactatt atcataggtg ggccaaatga ggataaccgg
    28261 atgaaaaaat gggcagtaat aatttctgca gtcggactgg cgtttgctgt ttccgggtgt
    28321 tccagtgatt acgtcatggc gaccaaagat ggccgtatga ttttgaccga tggaaaacct
    28381 gaaattgatg atgataccgg gctggtgagt tatcacgatc agcaaggtaa cgcgatgcaa
    28441 attaaccgtg atgatgtttc gcaaattatt gaacgttaac aaataaggtc agcatccggc
    28501 tgtaatgccg atcagttaag
//
LOCUS       DAZSBF010000030        28204 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZSBF010000030 DAZSBF010000000
VERSION     DAZSBF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28204
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.030"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHXWD_001008"
     CDS             complement(<1..643)
                     /locus_tag="ACHXWD_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR7619989.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="ACHXWD_001009"
     CDS             complement(743..1654)
                     /gene="gsiD"
                     /locus_tag="ACHXWD_001009"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR7619990.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="ACHXWD_001010"
     CDS             complement(1657..2577)
                     /gene="gsiC"
                     /locus_tag="ACHXWD_001010"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR7619991.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="ACHXWD_001011"
     CDS             complement(2595..4133)
                     /gene="gsiB"
                     /locus_tag="ACHXWD_001011"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR7619992.1"
                     /translation="MAKAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="ACHXWD_001012"
     CDS             complement(4153..6024)
                     /gene="gsiA"
                     /locus_tag="ACHXWD_001012"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR7619993.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="ACHXWD_001013"
     CDS             complement(6011..6976)
                     /gene="iaaA"
                     /locus_tag="ACHXWD_001013"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR7619994.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7180..8415
                     /gene="moeA"
                     /locus_tag="ACHXWD_001014"
     CDS             7180..8415
                     /gene="moeA"
                     /locus_tag="ACHXWD_001014"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR7619995.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8415..9164
                     /gene="moeB"
                     /locus_tag="ACHXWD_001015"
     CDS             8415..9164
                     /gene="moeB"
                     /locus_tag="ACHXWD_001015"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR7619996.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="ACHXWD_001016"
     CDS             complement(9343..10005)
                     /gene="fsa"
                     /locus_tag="ACHXWD_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR7619997.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10136..11035
                     /locus_tag="ACHXWD_001017"
     CDS             10136..11035
                     /locus_tag="ACHXWD_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR7619998.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11041..13473
                     /locus_tag="ACHXWD_001018"
     CDS             11041..13473
                     /locus_tag="ACHXWD_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR7619999.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13619..14434
                     /gene="ybiV"
                     /locus_tag="ACHXWD_001019"
     CDS             13619..14434
                     /gene="ybiV"
                     /locus_tag="ACHXWD_001019"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR7620000.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14586..15851
                     /locus_tag="ACHXWD_001020"
     CDS             14586..15851
                     /locus_tag="ACHXWD_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR7620001.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15818..16978)
                     /locus_tag="ACHXWD_001021"
     CDS             complement(15818..16978)
                     /locus_tag="ACHXWD_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7620002.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17216..18517
                     /locus_tag="ACHXWD_001022"
     CDS             17216..18517
                     /locus_tag="ACHXWD_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7620003.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18532..20895
                     /locus_tag="ACHXWD_001023"
     CDS             18532..20895
                     /locus_tag="ACHXWD_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR7620004.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21075..22670)
                     /locus_tag="ACHXWD_001024"
     CDS             complement(21075..22670)
                     /locus_tag="ACHXWD_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR7620005.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22889..23809
                     /gene="ldtB"
                     /locus_tag="ACHXWD_001025"
     CDS             22889..23809
                     /gene="ldtB"
                     /locus_tag="ACHXWD_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR7620006.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23868..24986)
                     /locus_tag="ACHXWD_001026"
     CDS             complement(23868..24986)
                     /locus_tag="ACHXWD_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR7620007.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="ACHXWD_001027"
     CDS             complement(24983..25450)
                     /gene="mntR"
                     /locus_tag="ACHXWD_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR7620008.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25636..25764
                     /gene="mntS"
                     /locus_tag="ACHXWD_001028"
     CDS             25636..25764
                     /gene="mntS"
                     /locus_tag="ACHXWD_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR7620009.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26036..27619
                     /gene="opgE"
                     /locus_tag="ACHXWD_001029"
     CDS             26036..27619
                     /gene="opgE"
                     /locus_tag="ACHXWD_001029"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR7620010.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="ACHXWD_001030"
     CDS             complement(27668..28183)
                     /gene="ompX"
                     /locus_tag="ACHXWD_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR7620011.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         6867 a         7774 c         7075 g         6488 t
ORIGIN      
        1 acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt ttacccggac
       61 gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa gccaccgacc
      121 acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg tcgacaagcg
      181 gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc cgcgaaaaga
      241 gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc atcatttcca
      301 ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac agtgagtcct
      361 ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt ttctgaataa
      421 gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga tagttaatga
      481 tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc agccttatct
      541 ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta tctgaacgct
      601 gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc gattctacaa
      661 aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg tgattaccct
      721 ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca tcgcgtaaac
      781 catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc ggaaaaacag
      841 cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct ccccactctg
      901 gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct ggcggcagag ataatcgagg
      961 tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc aagatatgac
     1021 gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata aaggtttgct
     1081 gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg gaaaaaatgg
     1141 cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca gcgatcgcca
     1201 gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcagc cgatcccacc
     1261 agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca ccgataaaca
     1321 cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg ctgaaaatgt
     1381 cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta ttcagattgt
     1441 cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa atggccacca
     1501 caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg cgaaatcgtc
     1561 gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc atggcgttta
     1621 acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga tagccgggtt
     1681 aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca gcgagaaaag
     1741 cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa cgagtaagcg
     1801 tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga gcaaaaagcc
     1861 aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc cgtgtttgag
     1921 gacaacccag gtttcgctca cccctttcgc cctcgcggta cgcatataat cttcgcttaa
     1981 aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg ccgcgccgag
     2041 cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag gcagccagcc
     2101 cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg gaaacgagat
     2161 ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc agacggcggc
     2221 gataattccc gccgccatac caaatatcac cgcccagacc atactggtta tggtcagcca
     2281 cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg acaccatcga
     2341 caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt gatacagcgg
     2401 ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag cttcgagccc
     2461 ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca ccagcaccga
     2521 gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat taagcattcc
     2581 actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt gtctggcatg
     2641 atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac caccagcggg
     2701 atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg ggtcttttcc
     2761 gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt attgctgtaa
     2821 aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg cgatagcgcc
     2881 cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac gccgctctct
     2941 ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc ggtcacctgg
     3001 gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt ctgcgcggtg
     3061 ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc cgcctctttc
     3121 agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg cgcgtaggcg
     3181 atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc aactttcacc
     3241 agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt atcgaacggc
     3301 ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc catcaactcg
     3361 atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa agcaaactgc
     3421 gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg acgccaggtt
     3481 atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt caccttcaca
     3541 aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg atgaaaacca
     3601 atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc cggatgggca
     3661 agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt tgtcggatcg
     3721 atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg attcgccgga
     3781 tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt gccatcctgg
     3841 aatttaatgc cttcccgcaa tttcacggtg taagtaatgc cgtcatcgga aacggtataa
     3901 ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag cccctggtaa
     3961 aacgatttcg ctacggcctg agataacgta tcattcgcgt catacggatc gagcgtggtg
     4021 aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga tgccatcaac
     4081 gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagcttttgc cattttattc
     4141 tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg cgacgtaatg
     4201 ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca gatgaatatt
     4261 gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg gttcagcgac
     4321 cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga agaccgcgcg
     4381 ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat gactaatccg
     4441 ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat cacgctggag
     4501 atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg aaacggcttc
     4561 gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga tgcgctggcg
     4621 ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca ggcccacgcg
     4681 ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac cgtgtacacg
     4741 cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag cgtaagggtc
     4801 ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg acaaggtatc
     4861 gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc gcagcaacgc
     4921 ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg tttcgccagg
     4981 ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc gattcaacaa
     5041 accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag gttcgccatc
     5101 caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa gcgatatcaa
     5161 cgggaaacgt cggggataat ccaacccttt catcgcacca agttgcggaa cagcagctaa
     5221 cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg tacccgtttc
     5281 caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca ccacgcccat
     5341 atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt tgattaattg
     5401 caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg caatcagcac
     5461 cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc cgccagagag
     5521 ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct gatccagcat
     5581 ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac gaattgattc
     5641 ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct cctgaaatat
     5701 catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct cgctaagttc
     5761 aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta aaccgcccgc
     5821 ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc cggattcgcc
     5881 aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga ccgcagctat
     5941 tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca cattaccggc
     6001 atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata ccggtggttg
     6061 gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac ggtagcgcga
     6121 cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca gggagttttt
     6181 ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc attaacgcgg
     6241 cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccgata caagaaaccg
     6301 ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg ccaactcgtc
     6361 cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg ccgtctaaat
     6421 ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc gcaccgctat
     6481 ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa cgcaaaggcg
     6541 tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa ttttctgccc
     6601 cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg gcaagaaccg
     6661 gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta ccatccatca
     6721 cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt ccggcgttaa
     6781 acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc gcactttcgc
     6841 ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca atgtagcgta
     6901 attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca tgaattgcaa
     6961 tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt gctataaata
     7021 tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga tatggcatgt
     7081 aaaggcagga gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt tttctgacat
     7141 aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac caccggattg
     7201 atgtcgctcg acaccgcgct taatgagatg ctttctcgcg tcaccccact gaccgcccag
     7261 gaaacgctgc cactggtaca gtgttttggt cgtattctgg cgagcgatgt cgtttcgccg
     7321 cttgatgttc cggggtttga taactccgca atggacggct acgcggtgcg tttagccgat
     7381 attgcctccg ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca gccgtatcat
     7441 ggtgaatggc ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt gccggaaggc
     7501 tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt gcgttttact
     7561 gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc tgcaggtgcg
     7621 gttgttttcc cggcgggaac tcgcctgact accgcagagc tgccagtaat tgcttcactg
     7681 gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc taccggtgat
     7741 gaactccagt tgcccggtca gccgctgggc gacggccaaa tctacgacac caaccgtctc
     7801 gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg gattatccgc
     7861 gacgatcccc atgccctgcg cgccgcattt attgaagccg acagccaggc ggatgtagtg
     7921 atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat tcttgaagag
     7981 ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt cgcgttcggt
     8041 aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc gacgctgacc
     8101 ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc cagcggcctg
     8161 cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg acgtcttgat
     8221 ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac gaccaccgga
     8281 catcagggtt cacatatatt tagctccttt agtctcggca actgctttat cgtgctggaa
     8341 cgcgatcgcg gcaatgtgga cgtgggcgaa tgggtggaag tagaaccgtt taacgcgttg
     8401 ttcggaggcc tgtaatggcg gaactcagcg atcaggagat gctgcgctac aaccggcaaa
     8461 tcattctgcg cggttttgat tttgacggtc aggaggcgct aaaagattct cgtgtgctga
     8521 tcgtcggcct cggtggactc ggctgcgcag cttcgctgta tctggcaagc gccggtgtcg
     8581 gcaacctgac gctgctcgac ttcgacacgg tttcgctctc gaatctgcaa cgccagacac
     8641 tgcacagtga tgccacggtc gggcaaccga aggtggaatc cgcccgtgac gccctgacgc
     8701 ggatcaaccc acatatcgcg attacgccag tcaatgcact gctagatgac gcagaacttg
     8761 cagcattgat tgctgaacac gatctggtgc tcgactgtac ggataacgtt gcggtacgta
     8821 atcaactgaa cgcaggctgt tttgccgcga aggtgccgct ggtttccggc gcggcaattc
     8881 gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc tatcgctgcc
     8941 ttagccgttt gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg atggcaccgt
     9001 tgatcggcgt aattggttcg ttgcaggcaa tggaagcgat caaactgctg gcaggttatg
     9061 gcaaaccgac cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag tttcgcgaaa
     9121 tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca cctccaggcc
     9181 tgataagacg cgacaagcgt cgcatcaggc atttgctcca gacgccagat gcggcgtgaa
     9241 cgccttatct ggcctacggt tcgggcacag atttccaagc gcggcaggca tcggtgcact
     9301 ccgccacgtc atcagcaatt tatgaggatg cagaacgtcc ggttaaatcg acgttctgcc
     9361 aaacgctccc tgccagtcct gctcaaactt cgccacagcg gcttccaccg ccggatagct
     9421 aatcatctgt tgtgccacat ccagtggcag agtaattgat tcacatcctg ccagtaagca
     9481 gtccagcgcc tgacgcgggg ttttgaaact cgctgccagc actttcgcct gcggcgcatg
     9541 cattttcaat aactggtgta agtcggtcac agtctgaatg ccgctaccgc cctgagcatc
     9601 aatacgatta acgtaaggcg caacatattc cgcacctgcc agcgccgaca gcaacccttg
     9661 tgctgcgcca tataccgcgg ttcccagcgt tggaatccct tccgctttta acatcttaat
     9721 agctgccagc ccctcggcgg tcaccggaac tttcaccacg atatccgcaa taatagaacg
     9781 caatttacgt gcgtcattca ccatcccttc agcagtggta gccattacct gggcaaacag
     9841 acgcccctga ccgcccatcg cttcatgaag ttgcggaagc acaacatcca gcggtttttt
     9901 acccgcggcg ataatgcttg ggttagtggt cacacccgcc agcggaaaaa tacgtgacag
     9961 cgccttcacc gcaacaacgt ctgaagtatc cagatacagt tccataacca tcctcaaaat
    10021 gtactttttg tctgaatgta gactatcacg ggaaaactct ctgaacagtt gacgcacatc
    10081 aatctaactt tcattcgaaa gtattttaat gtttgaacga aacagagagg cgactatgat
    10141 tttcaatatt cagcgctact cgacccatga tggccccggt atccgcacgg tcgtatttct
    10201 taaaggctgt tcgctgggct gccgctggtg tcagaacccg gaaagccgcg cccgcacgca
    10261 ggatctgctg tatgacgcac gactgtgtct ggaaggctgc gagctgtgcg ctaaggccgc
    10321 gccggaagtg attgagcgcg cgctgaatgg tttgcttatt catcgggaaa agttaacccc
    10381 ggagcatctg acggcgttaa ccgactgctg tccgacacag gcattaaccg tgtgtggtga
    10441 agtgaaaagc gttgaggaga tcatgacgac cgttctgcgc gataaaccgt tttacgatcg
    10501 cagcggcggc ggtttaacgc tttcgggtgg tgagcccttt atgcagccgg aaatggcgat
    10561 ggcgctactg caagccagcc acgaggcagg cattcatact gcggtagaaa cctgtctgca
    10621 tgtgccgtgg aaatatatcg ccccttctct gccctatatc gatctgtttc ttgccgattt
    10681 aaaacacgtt gccgacgcgc cgtttaaaca gtggaccgac ggtaacgccg ccagagtgct
    10741 ggataacctg aaaaaactcg ccgcagcggg caaaaaaatc attatccgcg tgccgctgat
    10801 tcagggcttt aatgccgacg aaacctctgt aaaagctatt accgattttg ccgccgacga
    10861 gctgcacgtt ggcgaaattc atttcctgcc ctaccacacg ctaggcatca acaaatatca
    10921 cttacttaat ctgccctatg acgccccgga aaaaccgctt gatgcgccag aactgctcga
    10981 ctttgcccag cagtatgcct gccagaaagg gttaaccgcg accttacgag gataacaacc
    11041 atgaccacac tgaaactgga cacgctcagc gaccgcatta aagcgcacaa aaatgcgctg
    11101 gtgcatattg tgaaaccgcc agtctgtacc gagcgcgcgc agcactatac cgagatgtat
    11161 caacaacatc tcgataagcc gatcccggta cgtcgcgcgc tggcactggc gcatcacctg
    11221 gcgaatcgca ccatctggat caaacacgat gagttgatca ttggcaacca ggcaagcgaa
    11281 gttcgcgccg cgccgatctt cccggaatat actgtctcgt ggatcgaaaa agagattgat
    11341 gatctggcag atcgtcccgg tgctggcttt gcggtgagcg aagagaacaa acgcgttctg
    11401 catgaagtgt gcccgtggtg gcgcggtcag accgtacagg atcgctgcta cggcatgttt
    11461 accgatgagc aaaaaggtct gctggcgacc ggaatcatta aagcggaagg caatatgacc
    11521 tccggcgatg cgcacctggc ggtgaatttc ccgctgctgc tggaaaaagg tcttgatggt
    11581 ctgcgcgaga aagtagcgga acgtcgctcg cgcatcaatc tgacggtact ggaagatttg
    11641 cacggcgaac agttcctgaa agcgattgat atcgtgctgg tggcagtcag cgaacacatt
    11701 gaacgttttg cagccctggc gcgtgaaatg gccgcgaccg aaacccgcga aagccgtcgc
    11761 gatgaactgc tggcgatagc agaaaactgc gatcttatcg cccaccagcc gccgcagact
    11821 ttctggcagg cgctgcaact gtgttacttc atccaattga tattgcagat cgaatctaac
    11881 ggtcactcgg tatcgtttgg tcgtatggac cagtatctct acccgtacta tcgccgcgac
    11941 gttgaactca accagacact ggatcgcgaa cacgccatcg aattgctgca tagctgctgg
    12001 ctgaaactgc tggaagtgaa caagatccgc tccggctcac actcaaaagc ctctgcggga
    12061 agtcctctgt atcagaacgt caccattggc ggacaaactc tggttgatgg tcaaccaatg
    12121 gacgcggtga atccactctc ttacgctatc ctcgaatcct gcggtcgcct gcgttccact
    12181 cagcctaacc tcagcgtgcg ttaccatgca ggaatgagta atgatttcct cgacgcctgc
    12241 gtacaggtga tccgttgcgg cttcgggatg ccggcgttca acaacgacga aatcgtgatc
    12301 ccggaattta ttaaactcgg tattgaaccg caggacgctt acgactacgc agcgattggt
    12361 tgtatcgaaa ccgccgtcgg tggcaaatgg ggctatcgct gtaccggcat gagctttatc
    12421 aacttcgccc gcgtgatgct ggcggcgctg gaaggcgggc gtgatgccac cagcggcaaa
    12481 gtgttcctgc cacaagaaaa agcgttgtcg gcaggcaact ttaacaattt cgatgaagta
    12541 atggacgcgt gggatacgca aatccgttat tacacgcgta aatcaatcga aatcgaatat
    12601 gtcgtcgaca ccatgctgga agagaacgtg cacgatattc tctgctcggc gctggtggat
    12661 gactgtattg agcgagcgaa aagtatcaag caaggcggcg cgaaatatga ctgggtttct
    12721 ggcctgcagg ttggcattgc caacctcggc aacagcctgg cggcagtgaa gaaactggta
    12781 tttgaacagg gcgcgattgg tcagcaacag ctggctgccg cactggcgga tgacttcgat
    12841 ggcctgactc atgagcaact gcgtcagcgg ctgattaacg gtgcgccgaa gtacggcaac
    12901 gacgatgata ccgtcgatac gctgctggct cgcgcttatc agacctatat cgacgaactg
    12961 aaacagtacc ataacccgcg ctacggtcgt ggtccggttg gcggcaacta ttacgcgggt
    13021 acctcgtcta tctccgctaa cgtaccgttt ggcgcgcaaa ctatggcaac accggacggg
    13081 cgtaaagccc acaccccgct ggcagaaggc gcaagcccgg cctccggtac tgaccatctt
    13141 ggccctactg cggtcattgg ctcagtgggt aaactgccta cggcagcgat tctcggcggc
    13201 gtgttgctca accagaaact gaatccggca acgctggaga acgaatctga caagcagaaa
    13261 ctgatgatcc tgctgcgtac cttctttgaa gtgcataaag gctggcatat tcagtacaac
    13321 atcgtttccc gcgaaacgct gctggaggcg aaaaaacatc cggatcagta ccgcgatctg
    13381 gtagtgcgtg tcgcgggcta ttccgccttc ttcaccgcgc tctctccaga cgcacaggac
    13441 gatatcatcg cccgtactga acatatgctg taatctcctc ggcccggcgg cgtcgtcgcc
    13501 gggctaaatc attcacatca tcaatttcat ccttactttc attcgaaata taatttgtgc
    13561 tctgcgtcac attgttatta cactgttacg gtttcatttt cgagccagga gcacacctat
    13621 gagcgtaaaa gttatcgtca cagacatgga cggtactttt cttaacgacg ccaaaacgta
    13681 caaccaacca cgttttatgg cgcaatatca ggaactgaaa aagcgcggca ttaagttcgt
    13741 tgtcgccagc ggtaatcagt attaccagct tatttcgttc tttcctgagc taaaggatga
    13801 gatctctttt gtcgcggaaa acggcgcact ggtttacgaa catggcaagc aactgttcca
    13861 cggcgaactg acccgacatg aatcgcggat tgttattggc gagttgctaa aagataagca
    13921 actcaatttt gtcgcctgcg gtctgcaaag tgcatatgtc agcgaaaatg cccccgaagc
    13981 atttgtcgca ctgatggcaa aacactacca tcgcctgaaa gctgtaaaag attatcagga
    14041 gattgacgac gtactgttca agttttcgct caacctgcca gatgaacaaa tcccgttagt
    14101 gatcgacaaa ctgcacatag cgctcgatgg cattatgaaa cccgtcacca gtggttttgg
    14161 ctttatcgac ctgattattc ccggtctaca taaagcaaac ggtatttcgc ggttactgaa
    14221 acgctgggat ctgtcaccgc aaaatgtggt agcgattggc gacagcggta acgatgcgga
    14281 gatgctgaaa atggcgcgtt attcctttgc gatgggcaat gctgcggaaa acattaaaca
    14341 aatcgcccgt tacgctaccg atgataataa tcatgaaggc gcgctgaatg tgattcaggc
    14401 ggtgctggat aacacatccc cttttaacag ctgacctctc taccggagcc gtgcgctccg
    14461 gttttccctt ctttttacat cctgtacatc acatcaaatc attaaacttg cattaactta
    14521 tttataactt atattgtcgc atgcaaatca gaatggtttc gaatgaaagt taatgagagg
    14581 tggttatggc ttctactttt accagcgaca cattgcctgc cgatcacaaa gcagctatcc
    14641 gtcagatgaa gcacgcgctg cgggcgcagc ttggcgacgt ccagcagatc tttaatcagc
    14701 taagcgatga cattgccacg cgagtggctg aaatcaacgc actcaaagca cagggcgatg
    14761 ccgtctggcc ggtgctgtct tatgccgata tcaaagcagg tcatgttact gcagagcagc
    14821 gcgaacagat taaacgtcgc ggttgtgcgg tgataaaagg ccatttcccc cgcgaacaag
    14881 cgctaggctg ggatcagtcg atgctggact atctggaccg caaccgcttt gacgaggtct
    14941 acaaaggccc cggcgataat ttcttcggga cgctcagcgc ttcacgtccc gagatttacc
    15001 ccatctactg gtcgcaggcg caaatgcagg cccgccagag tgaagaaatg gcgaatgcgc
    15061 agtcgtttct caatcgtctg tggacatttg aaagtgatgg aaagcaatgg tttaacccgg
    15121 atgtgagcgt catctaccct gaccgtatcc gccgccgtcc gcccggaacg acctccaaag
    15181 gtcttggagc gcataccgac tccggggcgc tggaacgctg gctgcttcca gcgtatcagc
    15241 gcgttttcgc caacgtcttt aatggcaatc tggcgcaata tgatccctgg catgcggcac
    15301 atcgtacgga agttgaagag tacacggtgg acaacaccac caaatgttcc gtgtttcgga
    15361 cattccaagg ctggacagcg ctctctaata tgctgcctgg tcaggggctg ctgcacgttg
    15421 tgcccattcc tgaagccatg gcgtacgtac tgttacgtcc gctgcttgat gatgtgccgg
    15481 aggatgaact gtgcggcgta gcgcccggaa gagtgttgcc ggtatcagag caatggcatc
    15541 cactgttaat tgaggcgtta accagcattc caaaactcga ggccggagac tccgtctggt
    15601 ggcactgcga cgtcatccat tccgttgccc ccgttgaaaa tcaacagggt tggggcaacg
    15661 tgatgtacat tcctgcggca ccaatgtgcg agaaaaatct tgcctacgcg cacaaggtga
    15721 aggccgcact ggaaaaaggc gcatcgccgg gcgacttccc gcgcgaggac tatgaaacaa
    15781 actgggaagg acgctttacg cttgccgacc tcaacattca cggtaagcga gcgttgggaa
    15841 tcgacagctg agtgtcgtac agcccttccc gttccacggc ggtgttccct tttcctgggc
    15901 gaacccgccg cactgactcc cgcaccgcca atcgtccgtg cagcagcagc gagcccaccg
    15961 gagcatccgg gcgaatcagc cgctgttgca acatttgcac cgcttcattg cctagctcat
    16021 cgcgtggcac atgcacagcc gtcaggggaa catcctcgat tgccgcaagg ttaaagccgt
    16081 cgatactcat caccgagaca tcctgtggta cgcgcagacc acgacgatgt agcgcattta
    16141 ccgttcccgc cgccataaaa tccccgccga ccagaaacgc cgtcggcaac gtcttgccat
    16201 tggcggcatc cagccacgct ccgacctttt cttccgcttc tttagcgcta aaactgctga
    16261 tattcagcaa atgacggtca tggtgaaagg ccagattatg ctgacgccat gattctttaa
    16321 tccctgcaaa ccgctgctcc atagtgtagc ggcgcaggca catcacattc atcacctccc
    16381 ggtgacccat ttcaaacaga tagcgggcgg cgaacgcgcc aattaaccga tgatccggcg
    16441 caatcgccgg gaggcgcatt cgttcatcac ggcagttgat cagcacacaa ggcttagcga
    16501 gatcggcggc cagatcatgg atgtgcggat cgtcaatccc cagcaggatc gctgcctgcg
    16561 tatctgcttc attcatccgc gagagaaaga gcgcgggatc gctgtcaaac tcctccagcg
    16621 cgcagtaacg cagccgcact tcatgcggcg ataacgcttt gctgatgctc tggatcacac
    16681 ggtaataaaa gatatcagat cgttggtcaa aagcgcgttg cggcgcgaaa ataatcaggt
    16741 tgttcagcaa catacgacct gctgccatgc catccatcac gcccaactca cgcgcgcagg
    16801 ccagcacctg ttcgcgagcg cgtgcactgg tattcgcttt gccagccagc acccgcgaaa
    16861 ccgtactcgg cgatagcccg gtacgtgcgg cgatatcggc gattttcagc ttcttgtcca
    16921 ttttgtgatc tgcctctaat tcggaaatga cagaattttc atgagcaaaa aagcccattc
    16981 tgtctggcat taaatccatt tcagtaatgt atatcagtca ctcatggaaa aacgtgcaca
    17041 aaacccgctt tctttcgctt ttagggtctc ttactgtgaa ttggtagaac aacttccata
    17101 caaaaataat ggaaaaacga acaataaaga ctttatttat caaaaagtta aaagtggtgt
    17161 aacaactcca ttctgacgtt gccgcacaaa ccctatacca ttctgtggag tgcatatgag
    17221 tcagggtatc aataacgata tgactgccag taaaccgcgc catatcgtca aaaatctgcg
    17281 ttggtggata ctggtccttt ttttgctcgg tgttactgtc aactacatca cgcgtaactc
    17341 gctgggtatt ctcgcccccg aactgaaaag cagcctaggg attaccaccg aacaatattc
    17401 ctggattgta ggtgctttcc agctcgccta taccctattc caaccgttgt gcggatggct
    17461 gattgacgtc attggcctca aactgggctt tatgatctgc gcgggactgt gggcactggc
    17521 ctgtctgatg catgcggggg cgggaaactg gggacatctg gcgctgttgc gcttctttat
    17581 gggcggtgcg gaagccgcgg caaccccagc aaacgccaaa accatcggtg aatggtttcc
    17641 aaaatcagaa cgcccgatcg ccgcaggctg ggcaggcgtc ggtttttcca tcggtgccat
    17701 gctggcccca ccgattatct attttgccca cgcttcattc ggctggcagg gtgcatttat
    17761 gttcactggc atcctggcga tgctgtgggt ggtgctgtgg tgggcgttct atcacagccc
    17821 ggataagcat cctaacctca gcaaagccga gctggacttt attcgccagg ataacgaagc
    17881 gcccccggtc aagttaccct ttctgaccgc actgaaaacc gtgggcaaaa ataaacgctt
    17941 ttacggtatc gctatcccgg cctttatggc agaaccggcg tgggcggtga tgagtttctg
    18001 ggtgcctcta tacctgtcca aaacctatgg catggacctg aagcagatcg cattattcgc
    18061 ctggttgccg ttcctcgccg ctgatcttgg cagtattgcc agcggctatc tgaccaaact
    18121 ttacacccgc ctgtttgggc tgagtcgtgt gaactccgtt gtcgcatcat cggtcagtgg
    18181 cgcattcttg atggtttcgc tggcggtgat ggcctttacc aataacccgt atatcgccat
    18241 cgtccttatc tccatcggcg gcttcggtca ccagatcatc tcctgcatgc tgagcgcact
    18301 ggtggtggaa tccttcgaca aaggtcagat ggccactgtt aacggcatgc gcggttctgc
    18361 cgcgtggatc gcaagtttcc tgttctccct gattatcggg gttaccgctg acaaaatcgg
    18421 ctttaacccc ctgtttatcg ccatgggctt ctttgacctg attggcgctt tcttcctggt
    18481 cacctttatt gctgaacgtc gcgcaaaacg cgcctgagag tggatgtaaa catgaaaacg
    18541 ctgaaaaact ggaccctgca caaacagtct gcccaccacg ttgaactgct ggtcgatggc
    18601 cagcatatcc tgtgcctgta cgtgctggaa gaaaatctgt tccgcgtgct gttaaaacgc
    18661 aaaggcgagc tggcgctaga tcgcacctgg agcattgctc cggcgcagga tgtgccatgg
    18721 gaagggcgca gccgcgaaga tctcagcggc tttagcctgc ccgcgtggac agtgatccag
    18781 cacgacgaca ccgtggttat cgccaccgag tcactgcgtg caaccgttca ccagccgctg
    18841 tggctggagt ggcactatcg taacaacgaa ggcgaatggc tgccgttggt gaatgaccgc
    18901 ccgaccagcg cttatctgct gaatgcccac ggcgatggcg tggcacacta tcagagccgt
    18961 cgtaaagatg aacgctttta tggcctcgga gataaagccg gcgatctgca acgcaacggt
    19021 aaacgttacg agatgcgtaa cctcgacgcg atgggctaca acgccgttag tactgacccg
    19081 ctgtacaaac acattccatt caccatcacc cgccgcgatg acattagctt cggcctgttt
    19141 tatgacaacc tcagcagctg ctggctggat ctgggcaacg agatcgacaa ctaccacacc
    19201 gcctatcgcc gctggcaggc ggaagcgggt gatatcgact actacctgtt taccggcgaa
    19261 cgcgtacttg atatcaccaa agccttcgta cgcctgaccg gaaaaaccct gttcggaccg
    19321 aaatggagcc tgggctacag cggttcaact atgcactaca ccgatgcgcc ggacgcacaa
    19381 aatcagttga tgaactttat ccgcctgtgt gaagaacatg caattccctg cgattcgttc
    19441 cagctctctt ccggttatac ctcaattaac ggcaaacgct atgtgttcaa ctggaactac
    19501 gacaaagtgc cgcagccgaa agtgatgagc caggcatttc acaatgctgg tttgcacctg
    19561 gccgccaata tcaagccgtg cctgttgcag gatcacccac gctataacga agtggcagaa
    19621 agaggactgt tcatccgtga ttcagaaaca gatgaaccag aacgttccag cttctgggat
    19681 gacgaaggtt ctaacctcga gtttactaat ccgcagacca tccagtggtg gcaggaaggc
    19741 gtgaccacac agcttctgga gatgggtatc gactccacct ggaacgacaa caacgaattc
    19801 gaagtatggg acggtgaagc acgctgtcac ggctttggtc agacgatcgc gatcaaacat
    19861 attcgcccag tgatgccact gctgatgatg cgagcctcga tggaagccca gcagcgtttc
    19921 gctccggaaa aacgtccgta cttgatctcc cgctccggct gcgcgggaat gcagcgctac
    19981 gtgcagacct ggagtggcga taaccgcact agctgggaca ctcttcgcta caacacccgc
    20041 atgggtctgg ggatgagcct ctccgggctg tataacctcg gtcacgacgt cggcggtttc
    20101 tccggtgata agccggaccc ggaactgttt gtacgctggg tacaaaacgg cgtgatgcat
    20161 ccgcgcttta cgattcactc atggaacgat gaccataccg taaacgaacc gtggatgtat
    20221 ccgggcgtca ctccggcgat ccgcagcgct attgagttgc gctatcgcct gctgccctac
    20281 ttttataccc tgatgtggca ggcatacgcc gatgatgaac caatgctacg tccgactttc
    20341 cttgaccacg agcacgacgt tcagactttt gaagagtgtg atgacttcct gcttggtcgc
    20401 gatattttgg ttgccagcgt cgtagagcaa ggcgagcgcc agcgccgtgt atggctgcca
    20461 gataacgaaa ctggctggta cgatttctac aacggcgaat ggttcagtgg cggtcagtgg
    20521 atcaccctcg acgcaccgct ggagaaactg ccgctgatgg ttcgtgccgg ggctggtcta
    20581 ccgctcagca aacgcatcac ctatgtgagc gcagaacaag acgatacccg cgaactgaaa
    20641 ctgttcccgc tgaaaggtgt gggcacaacg tccggcctgc tgtttgaaga cgatggcgaa
    20701 agctggggct accaaactgg taatgcgctt tgggtggaat gggaaatggt ttgcgatggg
    20761 gcgaccatca acctgaaagt caacgcgcgc ggggattatc gtcctgcatg gagtgcactg
    20821 aaagtgtcat taccattgga agaaaaacgc acgctgctgg tcaatggtgt tgaagggagt
    20881 gaatggatgc gttaaatcat taccggggcc gggaggcgac tgcaccttac ccggtccagc
    20941 catcgaatcc tttacctcag tatttcacgc tgcaggaagg cagcaaacct gtaaaccccg
    21001 ggagcttaaa taagtaagtg accggggtga acaggtgaag ccaatgcatc tgccgcatga
    21061 aagacggcgg ggaattaagc gtcgatccct ttactacgca ggtaatcttc gtaattaccg
    21121 ctaaagtcga tcgcgcgttc cggggtgatt tccagaatgc gggtcgccag ggagcttacg
    21181 aactcacggt cgtgtgaaac aaagatcagc gtgccctgat acagttccag cgccatgttc
    21241 agtgactcga tggattccat atccaggtgg ttggtcggtt cgtccatgat cagaatgttc
    21301 ggcttctgca tcattaactt accaaacagc atccgccctt tttccccacc ggaaagcact
    21361 ttagctggct ttttgatgtc gtcctggctg aacagcaaac gaccgagaat gctgcgtacc
    21421 gcctgctcgt catcgccttc ctgcttccac tgactcatcc attcgaacac ggtcagatca
    21481 ttttcaaact catattcgtg gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat
    21541 tttacggtgc cgctgtccgg ttgcagatcg cccaccagcg ttttcagcag cgttgattta
    21601 ccgacgccgt tggtacccag taccgcaagt ttttcaccga cctccagcag caggttgaga
    21661 tttttaaaca gtggaccgtt atcaaaccct ttggtcagac cttccacttc cagcgcgtta
    21721 cggaacagtt tcttatcctg ttcaaaacgg atgaacgggt tctgacggct ggaggctttc
    21781 acctcttcca gtttgatttt atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc
    21841 gaggcgttgg cgctaaagcg gctaacgaaa gattgcaact cagcaatctg cgctttcttc
    21901 ttggcgttat cggccagcag acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg
    21961 tagttacccg gataaacgcg cagctcgccg taatccagat ccgccatgtg ggtacagacc
    22021 atgttaagga agtgacggtc gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc
    22081 acctgttcca gccagcgaat ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg
    22141 agaatctccg gatcagcaaa cagcgcctgc gccagaagca cacgcagttt ccagccagga
    22201 gcaacttcac tcatcgggcc gtagtgctgt tccactggaa ttcccacgcc aagcaacagt
    22261 tcaccggcgc gagcttccgc agagtaaccg tccatttcgc cgtatttaac ttccagatcg
    22321 gccactttat agccgtcttc ctcactcatt tccggcaaag catagatgcg gtcacgctcc
    22381 tgcttcactt cccacaactc tttatgcccc atgatcaccg tatccagcac agtgaactct
    22441 tcaaaggcaa actgatcctg acgcagctta ccaatgcgct cgttgggatc gagggaaacg
    22501 ttacccaaag tcggctctaa gtcgccgccg aggatcttca taaaggtgga tttaccacta
    22561 ccgttcgcgc caatcaggcc gtaacggttg ccgccgccga atttgacgga aatgttttca
    22621 aacaacggct tactgccgaa ctgcatggtg acgttactgg aaactaacac aggcgtatcc
    22681 tgaaaagaga tatgacaaac cgcgcattat gccataagtc tgaaagaaga tcctgctcct
    22741 cgcttaaggt ctacactcaa gccaaaggca aaaaatgtga tttcgtacac atctgatttc
    22801 actgtgagct ggaatgaact tataatgcgc ttccaatact ctaatattct caacccaatg
    22861 gcctgccagg cacaaaatct cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc
    22921 ggccttcgct gttgtcggct tttgcagtac cgcctctgcg gtaacttatc ctctgccaac
    22981 cgacgggagt cgcctggttg gtcagaatca ggtgatcacc attcctgaag gtaacactca
    23041 gccgctggag tattttgccg cggagtacca gatggggctt tccaatatga tggaagcgaa
    23101 cccgggtgtg gataccttcc tgccgaaagg cggtactgta ctgaacattc cgcagcagct
    23161 gatcctgccg gataccgttc atgaaggcat cgtcattaac agtgcagaga tgcgtctgta
    23221 ttactatccg aaagggacca acaccgttat cgtgctgccg atcggcattg gtcagttagg
    23281 caaagatacg cctatcaact ggaccaccaa agttgagcgt aagaaagcag gcccgacctg
    23341 gacgccgacc gccaaaatgc acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt
    23401 cgttccggca ggtccggata acccgatggg gctgtatgca ctctacatcg gtcgcctgta
    23461 cgctatccat ggcaccaacg ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt
    23521 gcgtctgcgt aacgaagaca tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt
    23581 acagtttatt gatgagccgg taaaagcgac caccgagcca gacggcagcc gttatattga
    23641 agtccataac ccgctgtcta ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat
    23701 taccctgacg aagagcgtgc aaacagtgac cggtcagcca gatgttgacc aggttgttct
    23761 tgatgaagcg attaaaaacc gctccgggat gccggttcgt ctgaattaat cttcaaaact
    23821 taaagcaaaa ggcggactca taatccgcct tttttatttg ccagacccta gttggccggg
    23881 agtataacta acaaaacata tcccaccaac gccgcccaca acagcatcgg tattgaatag
    23941 agatggaaac gccaccagat gcggcgatcg ttcgccatac gtagcgcaat caaatttgcc
    24001 agcgatccgg gtaataaccc aaagccacct acgtttaccg cccataccag taataaagac
    24061 ggcggcacat agttcagcaa caatatggta ctcggcacgt tactgatcac ctgcgataaa
    24121 ccgattgccg ttaaccataa cccgggttca gatagatgac tcacgttacc caacacgcct
    24181 tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa acatcgccat aaacaccagc
    24241 agcagcgtcc agtccacact gagcaccacg cgacgtgcca gcagcgcaaa gcctgccgcc
    24301 acaatcacca gtccccacag ctcttgtttg aactccagcg ccgtcagaaa gacgatatac
    24361 agccccagac aactccacac cagccgcggt ttccactccg gtgtttgcac ccccgtatgg
    24421 tattgcagcg cctttccagg gaaacaacac cagcacagga gcaggagcgt cagcatcatt
    24481 gcgccagcca gcggtgccat ttgggcaata aatccggcaa acgaaagacc agaacgtccc
    24541 cagataagaa tattttgcgg gttgccaatt ggcgtcagta gcgaaccagc gttgactgcc
    24601 agcgcctcaa aaataatcag ccgattaacc gggatctcac acagtctttt tagcgtgata
    24661 gtcagcggaa caacaataaa cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc
    24721 gccgccagca ccataaacat cgccagccga cgctccgtag caaagcggcg caccattttg
    24781 cgccccagca catcaaaata accgcttaac tccacacctt tggtcagcag catcaggccg
    24841 cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc
    24901 acgaaaaagc ttaatccgat accaacaaga attaataact gaaaaaaacg atcgccttgc
    24961 agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag
    25021 cgtcgagcgt ctcttcacta acatggtgct ccatgccttc cgcgtcgcga cgggcgattt
    25081 ccggactgac gcccaacacc aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt
    25141 cctgcgccag cttctctcct tctgccgtta aaaacacgcc acgccagggg atcatttcaa
    25201 tcagccccat ggttgccagc cgcttaagca ttttagccac cgtcggttgc gaaactccca
    25261 gacgagcagc catgtccacc tgacgagctt cccccacttc cctgatcaag tcagaaatca
    25321 gctcaacgta atcatcaata agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt
    25381 caacgtgctc ttccacgttc actaactgcg tcactttttt tgctgttggc gtacctgcgc
    25441 gacgactcat tgtgcttcct cattatggtg acgcatacat agcgttctca tttcgtgcgc
    25501 acattgtaaa ccagagttgc gaaggtacaa aaaattaacg ttttagcaat agctacataa
    25561 tatagcctgt gctatatctg tatgtaatgc aatcatccct caaggatcga cgggattagc
    25621 aagtcaggag gtcttatgaa tgagttcaag aagtgtatgc gcgtgtttag tcattctccc
    25681 tttaaagtac ggttaatgct gctctctatg ttgtgcgata tggtcaacaa caaaccgcag
    25741 caagataaac cttccgataa atagcggcgt cgcggtacgc cgcttcactc ctgcttttat
    25801 gcaggcataa cgcgttttgg tctgaaaaac cccacttttt gtcggatttg caatcccctt
    25861 cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg ctcgctctat cttcttgcag
    25921 ccctacgtat attgcggctc gcggatgcgg acccctttcc cctcttcacg cactcttgca
    25981 ggtattgacc cttgacgcca gggtaagcac atggcgtttg ttacgatagt ggcatatgaa
    26041 tttaaccctc aaagaatcgc ttgttacccg tagccgggta tttagcccgt ggactgcgtt
    26101 ctacttttta cagtcgctat taattaacct cggcttaggt taccccttca gtttgctcta
    26161 caccgctgcg tttacggcta ttttgctttt gctatggcga acattgcctc gcgtacaaaa
    26221 agttctggtc ggtgtcagtt cgctggtggc ggcttgttat ttcccttttg ctcaggccta
    26281 cggcgcgcct aatttcaata cattgctggc attgcactcc accaatatgg aagagtcgac
    26341 cgaaatcctg acgatttttc cgtggtacag ctacctggtc ggcttattta tttttgcgct
    26401 cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa gcgcgctgga ataccttcga
    26461 cagcctgtgt ctggtattca gtgtggcgac attttttgtt gctcccgtgc aaaacctggc
    26521 ctggggtggc gtatttaaac tgaaagatac tggctatccg gtatttcgtt ttgctaagga
    26581 tgtcatcgtc aataataacg aggtgattga agagcaagaa cggatggcaa aactttccgg
    26641 aatgaaagat acctggacgg tcactgccgt taagccgaag tatcagacct atgtggtggt
    26701 gatcggcgaa agcgcgcgtc gcgatgccct cggtgccttt ggcggtcact gggacaatac
    26761 cccgtttgcc agcagcgtta acggtttgat atttgctgac tacattgccg ccagtggctc
    26821 cacgcagaaa tcacttggct taacgctcaa tcgcgtagtc gatggcaaac cacagtttca
    26881 ggataacttt gtcaccctgg caaatcgcgc gggcttccag acctggtggt tttccaacca
    26941 gggccaaatc ggcgaatacg atactgctat cgccagcatc gccaaacgtg cagatgaagt
    27001 gtacttcctg aaagaaggta atttcgaagc agataaaaac acgaaagacg aagcgttact
    27061 ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa ccgcagctga ttgttctgca
    27121 tctgatgggc tcgcatccgc aggcctgcga caggacacaa ggcaaatacg aaacctttgt
    27181 gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg caaacggacg atttactgcg
    27241 caagctgtac gatcagttac gcaacagcgg cagcagcttc tctctggttt acttttctga
    27301 ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa taccttgccc atgatgataa
    27361 atatcagcaa aatttccagg tgccttttat ggtcatttcc agcgacgata aagcgcatcg
    27421 ggtgattaaa gcccgccgct cagccaatga cttcttaggc tttttctcgc agtggacggg
    27481 aattaaagcg aaggaaataa atatcaaata cccgtttata tctgagaaga aagccgggcc
    27541 gatatacatc accaacttcc agttacagaa ggtggattac aaccatctcg gaaccgatat
    27601 tttcgacccg aaaccttaaa acaaaaaatc cgccccgaga ggcggatttt ttatatcacc
    27661 aaagtgatta gaagcggtaa ccaacaccgg caatccaggt gcctacgtca acgctacgaa
    27721 tacggctctg ctcgtaagag aagtccagag caacgttttc catcgggttg aactgcagac
    27781 cagcaccgta ggagaaaccg tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg
    27841 tctggaattt accataaccc acccccacta caccgtagat gcttgcccag tcgttaatgc
    27901 ggtaagccgg accagcagtg atgccgtagt actggttttt gttgtagtca ccagagcttg
    27961 cagtacggct tttctcggtg taagtgaaag aaccgatcac acccagcggg ctgttgtctt
    28021 cttcatagcg gtatttcagg ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc
    28081 tctgtgcgta accgccagtt acggtagaag tcgcagctac ggaagtacct gcggtgaaag
    28141 ccagaactgc ggccagtgct gaaagacatg caattttttt cataaccacc tcaaatgtga
    28201 ttca
//
LOCUS       DAZSBF010000031        28139 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZSBF010000031 DAZSBF010000000
VERSION     DAZSBF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28139
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.031"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHXWD_001031"
     CDS             complement(<1..215)
                     /locus_tag="ACHXWD_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR7620012.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHXWD_001032"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHXWD_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR7620013.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHXWD_001033"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHXWD_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR7620014.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHXWD_001034"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHXWD_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR7620015.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHXWD_001035"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHXWD_001035"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR7620016.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHXWD_001036"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHXWD_001036"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR7620017.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHXWD_001037"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHXWD_001037"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR7620018.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHXWD_001038"
     CDS             complement(4604..5326)
                     /locus_tag="ACHXWD_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7620019.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHXWD_001039"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHXWD_001039"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR7620020.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHXWD_001040"
     CDS             6526..6744
                     /locus_tag="ACHXWD_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR7620021.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHXWD_001041"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHXWD_001041"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR7620022.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHXWD_001042"
     CDS             complement(7478..7678)
                     /locus_tag="ACHXWD_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR7620023.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHXWD_001043"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHXWD_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR7620024.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHXWD_001044"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHXWD_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR7620025.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHXWD_001045"
     CDS             10175..12088
                     /locus_tag="ACHXWD_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7620026.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHXWD_001046"
     CDS             12088..13110
                     /locus_tag="ACHXWD_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR7620027.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHXWD_001047"
     CDS             13104..13322
                     /locus_tag="ACHXWD_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR7620028.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHXWD_001048"
     CDS             13376..14245
                     /locus_tag="ACHXWD_001048"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR7620029.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHXWD_001049"
     CDS             complement(14300..14704)
                     /locus_tag="ACHXWD_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR7620030.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHXWD_001050"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHXWD_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR7620031.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="ACHXWD_001051"
     CDS             15689..17779
                     /locus_tag="ACHXWD_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR7620032.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHXWD_001052"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHXWD_001052"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR7620033.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHXWD_001053"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHXWD_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR7620034.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHXWD_001054"
     CDS             complement(19747..20349)
                     /locus_tag="ACHXWD_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR7620035.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHXWD_001055"
     CDS             complement(20339..20506)
                     /locus_tag="ACHXWD_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR7620036.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHXWD_001056"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHXWD_001056"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR7620037.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHXWD_001057"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHXWD_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR7620038.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHXWD_001058"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHXWD_001058"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR7620039.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHXWD_001059"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHXWD_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR7620040.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHXWD_001060"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHXWD_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR7620041.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHXWD_001061"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHXWD_001061"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR7620042.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6797 a         7565 c         7115 g         6662 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatatt
//
LOCUS       DAZSBF010000032        27994 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZSBF010000032 DAZSBF010000000
VERSION     DAZSBF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.032"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHXWD_001062"
     CDS             complement(<1..123)
                     /locus_tag="ACHXWD_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7620043.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNE"
     gene            131..562
                     /gene="tnpA"
                     /locus_tag="ACHXWD_001063"
                     /pseudo
     CDS             131..562
                     /gene="tnpA"
                     /locus_tag="ACHXWD_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            622..696
                     /locus_tag="ACHXWD_001064"
                     /pseudo
     CDS             622..696
                     /locus_tag="ACHXWD_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(693..2016)
                     /locus_tag="ACHXWD_001065"
                     /pseudo
     CDS             complement(693..2016)
                     /locus_tag="ACHXWD_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2016..3632)
                     /locus_tag="ACHXWD_001066"
     CDS             complement(2016..3632)
                     /locus_tag="ACHXWD_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR7620044.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3928..4644
                     /locus_tag="ACHXWD_001067"
     CDS             3928..4644
                     /locus_tag="ACHXWD_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR7620045.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4641..6302)
                     /locus_tag="ACHXWD_001068"
     CDS             complement(4641..6302)
                     /locus_tag="ACHXWD_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR7620046.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6498..6926)
                     /gene="ibpB"
                     /locus_tag="ACHXWD_001069"
     CDS             complement(6498..6926)
                     /gene="ibpB"
                     /locus_tag="ACHXWD_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR7620047.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7038..7451)
                     /gene="ibpA"
                     /locus_tag="ACHXWD_001070"
     CDS             complement(7038..7451)
                     /gene="ibpA"
                     /locus_tag="ACHXWD_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR7620048.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7445..7517)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7757..8089
                     /locus_tag="ACHXWD_001071"
     CDS             7757..8089
                     /locus_tag="ACHXWD_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR7620049.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8091..9305)
                     /locus_tag="ACHXWD_001072"
     CDS             complement(8091..9305)
                     /locus_tag="ACHXWD_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR7620050.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9406..10470
                     /gene="cbrA"
                     /locus_tag="ACHXWD_001073"
     CDS             9406..10470
                     /gene="cbrA"
                     /locus_tag="ACHXWD_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR7620051.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10467..11804)
                     /locus_tag="ACHXWD_001074"
     CDS             complement(10467..11804)
                     /locus_tag="ACHXWD_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7620052.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11879..13027)
                     /gene="dgoD"
                     /locus_tag="ACHXWD_001075"
                     /pseudo
     CDS             complement(11879..13027)
                     /gene="dgoD"
                     /locus_tag="ACHXWD_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13024..13641)
                     /gene="dgoA"
                     /locus_tag="ACHXWD_001076"
     CDS             complement(13024..13641)
                     /gene="dgoA"
                     /locus_tag="ACHXWD_001076"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR7620053.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13625..14503)
                     /gene="dgoK"
                     /locus_tag="ACHXWD_001077"
     CDS             complement(13625..14503)
                     /gene="dgoK"
                     /locus_tag="ACHXWD_001077"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR7620054.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14500..15189)
                     /gene="dgoR"
                     /locus_tag="ACHXWD_001078"
     CDS             complement(14500..15189)
                     /gene="dgoR"
                     /locus_tag="ACHXWD_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR7620055.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15158..15790
                     /locus_tag="ACHXWD_001079"
                     /pseudo
     CDS             15158..15790
                     /locus_tag="ACHXWD_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010341111.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15836..16648)
                     /gene="yidA"
                     /locus_tag="ACHXWD_001080"
     CDS             complement(15836..16648)
                     /gene="yidA"
                     /locus_tag="ACHXWD_001080"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR7620056.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16763..17161)
                     /locus_tag="ACHXWD_001081"
     CDS             complement(16763..17161)
                     /locus_tag="ACHXWD_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR7620057.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17401..19815)
                     /gene="gyrB"
                     /locus_tag="ACHXWD_001082"
     CDS             complement(17401..19815)
                     /gene="gyrB"
                     /locus_tag="ACHXWD_001082"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR7620058.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19844..20917)
                     /gene="recF"
                     /locus_tag="ACHXWD_001083"
     CDS             complement(19844..20917)
                     /gene="recF"
                     /locus_tag="ACHXWD_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR7620059.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20917..22017)
                     /gene="dnaN"
                     /locus_tag="ACHXWD_001084"
     CDS             complement(20917..22017)
                     /gene="dnaN"
                     /locus_tag="ACHXWD_001084"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR7620060.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22022..23425)
                     /gene="dnaA"
                     /locus_tag="ACHXWD_001085"
     CDS             complement(22022..23425)
                     /gene="dnaA"
                     /locus_tag="ACHXWD_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR7620061.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23722..23802
                     /locus_tag="ACHXWD_001086"
     CDS             23722..23802
                     /locus_tag="ACHXWD_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620062.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24032..24172
                     /gene="rpmH"
                     /locus_tag="ACHXWD_001087"
     CDS             24032..24172
                     /gene="rpmH"
                     /locus_tag="ACHXWD_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR7620063.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24189..24548
                     /gene="rnpA"
                     /locus_tag="ACHXWD_001088"
     CDS             24189..24548
                     /gene="rnpA"
                     /locus_tag="ACHXWD_001088"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR7620064.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24512..24769
                     /gene="yidD"
                     /locus_tag="ACHXWD_001089"
     CDS             24512..24769
                     /gene="yidD"
                     /locus_tag="ACHXWD_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR7620065.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24772..26418
                     /gene="yidC"
                     /locus_tag="ACHXWD_001090"
     CDS             24772..26418
                     /gene="yidC"
                     /locus_tag="ACHXWD_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR7620066.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26524..27888
                     /gene="mnmE"
                     /locus_tag="ACHXWD_001091"
     CDS             26524..27888
                     /gene="mnmE"
                     /locus_tag="ACHXWD_001091"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR7620067.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6733 a         7573 c         7143 g         6545 t
ORIGIN      
        1 ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg cgaagcgcct
       61 catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt gtagtcgttt
      121 cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc gtggaaggca
      181 ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac gtcagatttt
      241 tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg ctgattttga
      301 agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa ttaactatcc
      361 tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg gtaggttact
      421 gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt tgtggagtcc
      481 tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc aatacattga
      541 acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc ctgaaggacg
      601 gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct aaagaatacg
      661 gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca gtacgctttg
      721 ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg taccgttttc
      781 gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc gtccacgacc
      841 agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg gatatcgccg
      901 accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac catcgcttca
      961 tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg ctcctgagtg
     1021 ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc aatctccagc
     1081 ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa aacctgtttc
     1141 tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat gctgaacaac
     1201 ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg ggtccagtgc
     1261 ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat gtgtatcttg
     1321 ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca tcaggtcgtt
     1381 gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt agtagcgcac
     1441 gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt caataccgat
     1501 tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg tggcttctgc
     1561 gacaatggct gccgggttgg agtagttgag catccaggca tttggtgaat atttttccat
     1621 ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta ttccgccggg
     1681 tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat ccagttcgcg
     1741 catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc tgaacgccac
     1801 ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa ggatgacttt
     1861 acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt tcagtgcacg
     1921 aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg taaaggtgct
     1981 tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt aatggcaaca
     2041 ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag ctgggataca
     2101 tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg cgctcccagc
     2161 tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat gcgtagacgc
     2221 gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg caggataccg
     2281 gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg cgaggctttg
     2341 tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt gcacatgttg
     2401 aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca caacccaatg
     2461 gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca gttttgcggt
     2521 aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag atacatcacg
     2581 gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga aatgaacagg
     2641 aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt tgcgggaatc
     2701 agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat cgctaaagaa
     2761 atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc ttccgggaac
     2821 aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc ccagtacatc
     2881 tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat gaagtggtgt
     2941 aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc aagcgcaccc
     3001 gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg ccagccgagc
     3061 aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac ataagacgtc
     3121 ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt atgcagcgcc
     3181 gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt tttaatcccg
     3241 gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac gccgaagtag
     3301 cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa actcaccatc
     3361 accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa accgacagca
     3421 aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat gtgtacgatt
     3481 tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg gttttgcagc
     3541 aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg cgtaaacatc
     3601 gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct caattctgta
     3661 agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag atgcgatcgg
     3721 cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt gggcggtaga
     3781 gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc atcgaacaca
     3841 tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg cattacccgg
     3901 gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga gcggttaaga
     3961 attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga aaaaaagctg
     4021 gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct gctggtagcg
     4081 tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa agatgtactg
     4141 catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg aaaaactgtc
     4201 accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc cagccagtta
     4261 cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt ggaagggaaa
     4321 ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct ttcgctgcaa
     4381 catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt gattggcggt
     4441 aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca aatgaaggta
     4501 gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag cggggagttt
     4561 ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta ccatttacgg
     4621 cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag taattggggg
     4681 gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag cgccgccgca
     4741 ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt catggaacct
     4801 gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc cagaatgcca
     4861 acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct tagcccttcg
     4921 ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac cgagaggaac
     4981 agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa ccagtacagc
     5041 ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc gcccgccagc
     5101 cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc taacaatacg
     5161 ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt ttgctgcgca
     5221 ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag gttgaggata
     5281 tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc acggttcagg
     5341 cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg ttttccgagc
     5401 acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt cgtggacagc
     5461 gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc tggctgaccc
     5521 accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac ttttagggtt
     5581 tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg tacatcttta
     5641 atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt gatcagcgcg
     5701 ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt gacgcggaaa
     5761 ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg atacgccatc
     5821 gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc gcgcaaaatc
     5881 tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat ccccagcact
     5941 accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc accgatgatg
     6001 acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga ggcaaagaag
     6061 cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa ttcctgaata
     6121 acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg gccgacgatg
     6181 atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt gacgttgccg
     6241 ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc tatatcactc
     6301 atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg aatcggtgaa
     6361 gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat atcttaaaca
     6421 gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca gaatggcggg
     6481 gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc gatacgctgc
     6541 gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc gtttacgaaa
     6601 gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa tggctgattc
     6661 ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt gccttttacg
     6721 ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc tgccagcgca
     6781 agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg gaagctctga
     6841 ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc
     6901 agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg agtaagtacc
     6961 tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt caggccgcgc
     7021 caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc ttccggaatc
     7081 acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg aacatgaatg
     7141 ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg gtacagatag
     7201 gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt atcctgggcg
     7261 gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg gtaatggttt
     7321 tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct ctggttgttt
     7381 tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg ggataaatca
     7441 aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt ccacaatgga
     7501 caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat gtaaaaatgg
     7561 gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt tatggtctat
     7621 tgcatagact gagggggcag cacgaaacgt taaagtgtga tagccgccac agagcgacgt
     7681 aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac acccacgatc
     7741 aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat gcagcggaat
     7801 ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag aaggaacgta
     7861 tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact ggggcactaa
     7921 gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt tattaccgtg
     7981 ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg aagccaacgc
     8041 gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat ttagcgatca
     8101 gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc atccggcgag
     8161 aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata ctcaacctcg
     8221 ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa ccattctcct
     8281 gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa ctggcgcggc
     8341 tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc acgcattaaa
     8401 aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac gttgactaac
     8461 cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc acgcggtggc
     8521 gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt ccagccagct
     8581 tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt tggcgaaagt
     8641 gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata agcacgattg
     8701 atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac acaccagtgg
     8761 ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa cggcgcagca
     8821 acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg gtcgttatag
     8881 gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc gccgcgcagc
     8941 gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac cttgccgcct
     9001 tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc aggattttcc
     9061 gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac gccgacgtgt
     9121 gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt cacacgctca
     9181 atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag ccactggctg
     9241 tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt
     9301 ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa ggacttcact
     9361 ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac
     9421 gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa
     9481 atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt cagcaaacct
     9541 tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg gctgacgctt
     9601 cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga cgtcgccgca
     9661 tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg
     9721 tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct gtgtcgaaaa
     9781 atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcagcat
     9841 attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg gcgacatctc
     9901 tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc agagaaacat
     9961 ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta ttcatggagt
    10021 atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg
    10081 cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg
    10141 aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt ctgcggtaag
    10201 gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg
    10261 attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa gcagccagag
    10321 aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact cttcggcaag
    10381 atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt
    10441 gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc caccagcagg
    10501 atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggtgcgaaa
    10561 ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg
    10621 gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagaaacc
    10681 agcgaccagg taattgaagc aaaaccgtta ccgaagaatg ccagcgccat caggcacata
    10741 atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat cagcaagccg
    10801 cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc
    10861 gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata
    10921 aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt
    10981 aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc gatcagttta
    11041 cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc tttcttcact
    11101 ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg
    11161 atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg
    11221 ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg aatccagatc
    11281 agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg
    11341 gaggcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg
    11401 ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca
    11461 aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata agtcacgcga
    11521 gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg
    11581 aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg
    11641 gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc
    11701 atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata
    11761 gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag
    11821 agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt
    11881 accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag tccggggcat
    11941 ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc ggtttcgtta
    12001 gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta
    12061 actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc gcgttatagg
    12121 aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga cagtgcggcg
    12181 caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg gtaataccgc
    12241 ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc acgcgtttaa
    12301 aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca
    12361 atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat
    12421 acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat
    12481 caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg
    12541 ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga
    12601 aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc gccggacgat
    12661 cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc
    12721 agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg atcccggcaa
    12781 tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata
    12841 agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg
    12901 cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg acgccttcat
    12961 cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga
    13021 ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg
    13081 ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag cacaacctgc
    13141 gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc
    13201 cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa
    13261 tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca
    13321 gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac tatggatatt
    13381 gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag
    13441 taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct
    13501 ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac
    13561 cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg cgatcagcgg
    13621 aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc
    13681 ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca
    13741 acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg cgcatactgg
    13801 cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca cggggaagtg
    13861 ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg ggcgtattaa
    13921 gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc ggcaaacctg
    13981 caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa
    14041 aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcatgacat
    14101 aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg ccgcgcatca
    14161 cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata ttgtcgccaa
    14221 cagacgttaa ttgttcgcca atagacgaaa aatgggcagg aacagataaa tacggagcaa
    14281 ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta ttctcttcac
    14341 gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc aggcgcgtga
    14401 cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca
    14461 gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct
    14521 tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg
    14581 ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc
    14641 gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg
    14701 ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc
    14761 gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg
    14821 cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag
    14881 atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca
    14941 ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgcgccgcg
    15001 ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct cacggatgat
    15061 gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg gcgagcctgg
    15121 tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat cggttttatt
    15181 gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc
    15241 ataatcagga aagtatcaat cgcagtcaga caccatcacg gggctatcgc aggcaaagga
    15301 tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc ttatcacaga
    15361 tggtgccgat gacatcgtta agatgctcaa ggatccagct cttaaccggc acaatattca
    15421 ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta ccggcacaat
    15481 atcactctat tacaactgga gcacagaaga agaaaaagca cgagtgacac aatatcgttt
    15541 tgcttgtggt gttcagcact gtacccggac agtagaaaac ctggaaggta ctatccatga
    15601 gaaaaataaa aacatggatt actcaaacgt aatggctttc taccatccat ttaaagtgcg
    15661 attttatgaa taccattcac ccagaggcat tccggatggt gtttccgcag cattactgcc
    15721 agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg tatatgcagt
    15781 aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc acagattaat
    15841 tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttagat ttggtgacaa
    15901 agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg acgcctgcat
    15961 attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct tccggtttaa
    16021 tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt ttatcgagga
    16081 tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact tcctgcggaa
    16141 tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact ttcaggaact
    16201 gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatagtg gcaacgaaag
    16261 attcatgcac tgtgtagtag ctgatatcgc ggttggcggt gtacagcgtg gtgcggtcca
    16321 gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga tagtcgtcat
    16381 agctgagagc agtttgcgcc acggtgctac catcagctgc cttctgtact agcgcgccgt
    16441 tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg tagttgtgca
    16501 cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg cgagctgcgg
    16561 caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca
    16621 tatcgatagc aatgagtttg atagccatga gttccccagg tagattggtt cctgacccat
    16681 gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg gatgcaacgc
    16741 gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt tccatacctg
    16801 cggaaagcag atcgttattg gcttgtggtg acacttcacc ttgcggtgag agcgcatcaa
    16861 taatcttcgg caattgctct gccagtaaac tggaagctgt actggtatcc acgccaagtt
    16921 tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc tcgccactaa
    16981 ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct aagccgccac
    17041 tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa cttaaaatag
    17101 cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca aaaagcccca
    17161 tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatttg cgggatgata
    17221 attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct tatagcttct
    17281 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgaaaatt caatacattg
    17341 caagattttc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg gttagcgcca
    17401 ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac ggcgcggttc
    17461 aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca gcaatcgcat ctttaacggt
    17521 aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct gttccgggtt
    17581 catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact ctttcaccag
    17641 ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac gttcgataaa
    17701 cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac gacgatattc
    17761 gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt gggtacgcac
    17821 gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg tggacatcaa acttccactg
    17881 gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc agcgggtaac
    17941 ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa gctctttcag
    18001 cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg cgttgtactc
    18061 agacaccaat ttctctaacg cttcgccagc caatgccggt gcgctggcgt tggtgtgcag
    18121 cgttgcgcca tccagcgcga tagagatctg gtactgatcc atcgcttcgt cgtctttaat
    18181 gtactgttct tgcttgcctt tcttcacttt gtacagcggc ggctgagcga tgtagacgtg
    18241 accgcgttca acgatttccg gcatctgacg atagaaaaag gtcaacagca gcgtacgaat
    18301 gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac gcagtttgtc
    18361 cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg tcgccacttc
    18421 ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt tacccttcag
    18481 cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc ccgcggagtc
    18541 cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt ctgccagttt
    18601 gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac gcgcgcgacg
    18661 cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt tcgcgtcggt
    18721 tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa ccgctgattt
    18781 cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt ccggcacttt
    18841 cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc taactttggc
    18901 ttttttgctg tagccttctt tgtccatgta ggcgttcagg gtacgggtca tcgccgcacg
    18961 gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa agcagtagat
    19021 gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac cgtctttttc
    19081 agtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca gatattcaac
    19141 gaacgccttg atgccgcctt catagtggaa gtggtcttct ttgccgtcgc gcttgtcgcg
    19201 cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt tcgccagaat
    19261 ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga aacgcaccat
    19321 ggtgccggtt ttttcagtct cgccggtaac cgccaacggt gcctgcggta caccgtgttc
    19381 gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt tttgcgacag
    19441 ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat aggagttatc
    19501 gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg atacgccctc
    19561 ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga cagagttatc
    19621 ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt cgatagcgtt
    19681 atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt cgccgatata
    19741 catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac tggaggagtc
    19801 ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc gttattttac
    19861 ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg tgttcagcac
    19921 tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca agcagcccgc
    19981 gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac cgccgcccgc
    20041 tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac agcttaagct
    20101 gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt aagtccgctt
    20161 tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta cgttccagca
    20221 cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc agagaaaact
    20281 cagggagaaa ttgcttacaa gtatcggcca tatcagccgc gataccggcg ctatactccg
    20341 cgcgccaggt gctgatttgc tccgccagcg ggatcagctc tttatcccac gggcgtagct
    20401 gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat cgcttgagat
    20461 tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg aggaatgctc
    20521 ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc aactgcattg
    20581 gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt accgtcgatg cggactttgc
    20641 tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc gcgctcttcg ccctgtaatc
    20701 gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac gcgaccaatc tgcaaactgc
    20761 gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt ttgccactgc
    20821 cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg gtttcaatgt
    20881 tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg gcatgacaac
    20941 ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg aatcggtcag
    21001 catcatgcgg acgttttcac atttcagcgc gttcagaaca tccagcacgt agctgacgtt
    21061 gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt ccgcttcttc
    21121 ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataaa gacgtacgcc
    21181 gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga gcagatcgca
    21241 gccagcttcc agatgtttgt ccgggttctt cggcagaacg cggcgataat ccgggaagcg
    21301 accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa tgttgttgct
    21361 gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca gttcaatcac
    21421 gcctttacgc ggcacgatca ccgaatggct tggcaaagat tgaccaattg gcattgaaca
    21481 gactgccaga cggtggccgt cggtcgccac ggtgcgcagt tcttcgcctt cggtttcaaa
    21541 cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact gggtcgcttc
    21601 aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct gccagtcatc
    21661 gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac gcccggagcg
    21721 caccagcatc cgttcacctt ccagctgtac ggcaatttcc gcgccttcag gcagaccacg
    21781 gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt gtggctgaac
    21841 cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg acaacgtacc
    21901 gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac cacctaacgg
    21961 cccgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa atttcatagg
    22021 tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg tggctctctt
    22081 cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca cgaccaccaa
    22141 acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc gccatcgcca
    22201 tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc gcgactttga
    22261 tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt ttttcctgca
    22321 atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat ggtgatcgcc cgtccggtaa
    22381 agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg ttagatcgta
    22441 gacgcttggc gataaagaac gccacttcgc ctggcaaacg aatgtcattt tcgtcggcct
    22501 ttttcatcag gatcgccacg cgggtttcca gctctggcgg ttcgatcgcc acagtcagtc
    22561 cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc ggatagcgat
    22621 ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg tggaaaaact
    22681 cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc agtgcatcta
    22741 cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct ttaaccatgt
    22801 cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg cgcgccataa
    22861 tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg ccataaagga
    22921 acaatgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc gcgcgcgcca
    22981 gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt acgttagaac
    23041 gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta gaaggcgcag
    23101 cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc gtcactgccg
    23161 cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt ggggcatccg
    23221 ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc cgtacccaat
    23281 cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt tccgcctgca
    23341 atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat cgggcaagac
    23401 actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc gataatgact
    23461 aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg agggtgacgc
    23521 acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat cgggaccgcg
    23581 gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc cgatttatcc
    23641 acaggacttt ccagacctcg ctaagtgtaa acgatcctgc cgcgaggcgg gcacgattta
    23701 cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg atctaataaa
    23761 gatctaggac gatccttgcg ctttacccat cagcccgtat aatcctccac ccggcgcgcc
    23821 atgctggttt tcactggtgt gaggtcgtac attttccctg cgaaaaggtg cggaaaagcg
    23881 cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc cggcctcttt
    23941 aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca ataaagccat
    24001 tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac cgtctgtact
    24061 gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg gtcgtcaggt
    24121 tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt aataaagcta
    24181 acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact cccagtcaat
    24241 tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc attctcggcc
    24301 gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa aacgttcgac
    24361 gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt ctgcgccaac
    24421 atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc gacctcgata
    24481 accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc ctggctcgcg
    24541 ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac tcgggccgca
    24601 ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca ggtttggagt
    24661 gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt tacaccctgg
    24721 tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac gatggattcg
    24781 caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg gcaagcctgg
    24841 gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac gaccaccgca
    24901 gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact gatctcggtt
    24961 aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga gcaagctctg
    25021 ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct ggaaacttca
    25081 ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc ggataacccg
    25141 gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct ggctgaaggt
    25201 caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac gtttaccaaa
    25261 acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt gcagaacgct
    25321 ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat cactctgcca
    25381 ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg cgcggcgtac
    25441 tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga taacgaaaac
    25501 ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc aacagtattt cgcgacggcg
    25561 tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg taacggcatc
    25621 gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac tggcgcgatg
    25681 aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt tgctccgcac
    25741 ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct gttcaaactg
    25801 ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat catcaccttt
    25861 atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat ggcgaagatg
    25921 cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga caaacagcgt
    25981 atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct gggcggctgc
    26041 ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct gatgggttcc
    26101 gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc acaggacccg
    26161 tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa gatgtcgccg
    26221 accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt catcttcacc
    26281 gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag caacctggta
    26341 accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct gcatagccgc
    26401 gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc agttgaccgc
    26461 cttttttctt ttcgtagggt ggataagcac cgcgcatccg ccacacaaag caacaggaac
    26521 atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg tggcggcgtt
    26581 ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg ccgaaaccgt gctgggtaaa
    26641 ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg cagcgtgctc
    26701 gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga agatgtgctg
    26761 gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg cattctgacc
    26821 attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt tcttaacgat
    26881 aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag ttcggaacag
    26941 gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt caaccatctg
    27001 gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt ccctgatgaa
    27061 gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt gattgccgat
    27121 ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg gatgaaggtg
    27181 gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct ggcgggccgt
    27241 gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg tgagcatatc
    27301 cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga agccagtgac
    27361 gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc cgaccgcgtg
    27421 ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat ctggccggaa
    27481 tttatcgccc gtctgccagc gaaactaccg atcaccgtgg tgcgcaataa agccgatatc
    27541 accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg tctctcggca
    27601 aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca aacagagcat gggctttgac
    27661 accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct ggaacaggca
    27721 gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg cctgggcggg tgaactgctg
    27781 gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga atttacctct
    27841 gatgatttgc tggggcggat tttctccagc ttctgtattg gtaagtaacc gcgcttacga
    27901 agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct gttattcctc
    27961 aacccttttt ttaaacatta aaattcttac gtaa
//
LOCUS       DAZSBF010000033        27152 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZSBF010000033 DAZSBF010000000
VERSION     DAZSBF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27152
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.033"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHXWD_001092"
     CDS             <1..411
                     /locus_tag="ACHXWD_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR7620068.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(459..1331)
                     /locus_tag="ACHXWD_001093"
     CDS             complement(459..1331)
                     /locus_tag="ACHXWD_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR7620069.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(1343..2437)
                     /locus_tag="ACHXWD_001094"
     CDS             complement(1343..2437)
                     /locus_tag="ACHXWD_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR7620070.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(2470..3468)
                     /locus_tag="ACHXWD_001095"
     CDS             complement(2470..3468)
                     /locus_tag="ACHXWD_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7620071.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(3493..5004)
                     /locus_tag="ACHXWD_001096"
     CDS             complement(3493..5004)
                     /locus_tag="ACHXWD_001096"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR7620072.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(5027..6010)
                     /locus_tag="ACHXWD_001097"
     CDS             complement(5027..6010)
                     /locus_tag="ACHXWD_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR7620073.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(6107..9384)
                     /locus_tag="ACHXWD_001098"
                     /pseudo
     CDS             complement(6107..9384)
                     /locus_tag="ACHXWD_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            9502..10695
                     /locus_tag="ACHXWD_001099"
                     /pseudo
     CDS             9502..10695
                     /locus_tag="ACHXWD_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            complement(10893..12146)
                     /gene="glyA"
                     /locus_tag="ACHXWD_001100"
     CDS             complement(10893..12146)
                     /gene="glyA"
                     /locus_tag="ACHXWD_001100"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR7620074.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            12474..13664
                     /gene="hmpA"
                     /locus_tag="ACHXWD_001101"
     CDS             12474..13664
                     /gene="hmpA"
                     /locus_tag="ACHXWD_001101"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR7620075.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(13709..14047)
                     /gene="glnB"
                     /locus_tag="ACHXWD_001102"
     CDS             complement(13709..14047)
                     /gene="glnB"
                     /locus_tag="ACHXWD_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR7620076.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(14108..15442)
                     /gene="glrR"
                     /locus_tag="ACHXWD_001103"
     CDS             complement(14108..15442)
                     /gene="glrR"
                     /locus_tag="ACHXWD_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR7620077.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(15432..16145)
                     /gene="qseG"
                     /locus_tag="ACHXWD_001104"
     CDS             complement(15432..16145)
                     /gene="qseG"
                     /locus_tag="ACHXWD_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR7620078.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(16310..17737)
                     /gene="qseE"
                     /locus_tag="ACHXWD_001105"
     CDS             complement(16310..17737)
                     /gene="qseE"
                     /locus_tag="ACHXWD_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR7620079.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(18295..22182)
                     /gene="purL"
                     /locus_tag="ACHXWD_001106"
                     /gene_synonym="purI"
     CDS             complement(18295..22182)
                     /gene="purL"
                     /locus_tag="ACHXWD_001106"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR7620080.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            22440..23996
                     /gene="mltF"
                     /locus_tag="ACHXWD_001107"
     CDS             22440..23996
                     /gene="mltF"
                     /locus_tag="ACHXWD_001107"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR7620081.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(23993..24496)
                     /gene="tadA"
                     /locus_tag="ACHXWD_001108"
     CDS             complement(23993..24496)
                     /gene="tadA"
                     /locus_tag="ACHXWD_001108"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR7620082.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(24554..25189)
                     /gene="yfhb"
                     /locus_tag="ACHXWD_001109"
     CDS             complement(24554..25189)
                     /gene="yfhb"
                     /locus_tag="ACHXWD_001109"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR7620083.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            25398..26246
                     /locus_tag="ACHXWD_001110"
     CDS             25398..26246
                     /locus_tag="ACHXWD_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR7620084.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(26585..27142)
                     /gene="pinE"
                     /locus_tag="ACHXWD_001111"
                     /pseudo
     CDS             complement(26585..27142)
                     /gene="pinE"
                     /locus_tag="ACHXWD_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         6480 a         7469 c         6839 g         6364 t
ORIGIN      
        1 cgttattttg attttggagc tgcccgcccc gttttgttat taattgcccg tatcgccgtg
       61 gtcttaattt tcattatttt tggttttccc aaaatgatgg gctttgacgg tacggtccaa
      121 tatatggcct cgttgggcgc gccaatgccg atgctggcag cgattattgc ggtagttatg
      181 gaagtgcccg ccgcgatatt aatcgtgctt ggctttttca cccgtccgct ggcggtgctg
      241 tttattttct acacgctggg tacggcggtg attggtcacc attactggga tatgaccggc
      301 gatgcggttg ggccaaatat gattaatttc tggaagaatg tcagtatcgc tggcgcgttc
      361 ttgctattgg caattaccgg gccgggggca atttctctcg atcggcgtta ggaaagatgc
      421 cggatgcggc gtgaacgcct tatccggcat ttaataaatt acagccactc tacccgcaac
      481 gacatctctg aagttgttga ttcccctggt gctagcgcaa tgagatcacc cccttccggg
      541 cgatgatgat catccggcgc atggctcatc ggttccagac agaagaaatc aaacgcatat
      601 cctttatcaa acgcagggtc ggaaacaaag atgaaataac acggtgcagg tggcgtcgtt
      661 tccatgatga tggcataacc ttcctgcggc tgctcgatgc gggcctgacc attccatccg
      721 gcaaaaccat tgttcaccca ctggcgcggc aacggcgcga gctggttaaa atccagttcc
      781 tgcggtagct gctcgcaaaa ctcacccgcc agccactgct cccgttccag ccagtaaccg
      841 ctcgcctgcg cctgaatccg cgtttgtggt gacaacggaa aataaggatg ccagccggta
      901 ccaaatggca gcgtctctgc cccttgattg gtgacagaga gcgtcaccgt cagcgtatcc
      961 gccgttaaat gaaacgcctg acttacccga tagtgataga caccgctgcg atgttcatac
     1021 accagacaca aactatcatc gctgtgcgag acacattgcc actcgcccag ccagccatcg
     1081 ccgtgtagat agtgcgcatc ccactcaaca ttcggttgca gttgatactc acgcccctgc
     1141 cagacaaaac ggttgccgct cacccggttt gcaaatggca ccagcggaaa acatgaggca
     1201 tcggttgcca caccgctttt tttaccagga cgtaagagcg gcgtcgtatc gcgccagaag
     1261 ccttcgatta cgccgccctg gtcagaaacg tccagcttta gcgacccgtg ggataaggta
     1321 tagatggtca tccgctaact ccttaatccg ggaagttaat cacaactttc ccgcatttgc
     1381 cgctcgccat cagcgcatag gcatcacctg cctgttccag cgagaagcga tgggtaatgg
     1441 cgttacgcgg ccacagcttc cagtcagtca aatcgtgggc gcatttttcc atatggaaca
     1501 gactggtcac ccaggagccg ataatccgcc gttgatggtg catcagatcg gcgctgacct
     1561 cgaattccac ttttccggtt tcaccaatgt aaaccacccg tccccagtca gcggtggatt
     1621 gcagtgccag caagcgacct gcggcattac cagaacaatc gagcgcaaca tccgcgccac
     1681 cgtgggtgag ttcggcgata atctgcggca gaccttcggt ggttgctaaa tagccgtgat
     1741 ccatcacccc taactgtttt gccatcgcca gacgttccgg cagcatatca acgccgatga
     1801 tccgttttgc accgcgacct ttcgccagca tcatcgccat catgccgacc ggccccagac
     1861 cgaccaccag cacgttatcg ctgccggaaa cttcgccgcg caaaatccct tcataggccg
     1921 tgccaacgcc gcaactgata aacgcaccat cttcgtagct cagcgcatcc ggcaggagga
     1981 tcagatcttt ttcttccgcc agcagatatt cggcatgacc gccgtcacgc tgccagccgt
     2041 aagccgcttt tcctttgcca gtacaagaga taggaaagcc gcggcggcag ttcgggcaaa
     2101 aaccacagcc agaaatgtga tacaccagca cgcggtcgcc ctctttaaaa tggcggcagc
     2161 cttgccccat cgccacaatc tgcccgcacg gttcatgacc gttgataaat ccctggtata
     2221 acggtttatc gggtgccgcc gccgtggcac ggtgttgatg atagatatag tggacatcgc
     2281 ttccgcaaat cccggaggat ttcattttga tcagtacctg gttaatcccc ggcgtcggca
     2341 ccgcaacttc ccgcagatcg acggttgaat ttcctggtaa ataagctgcc agcatcgttt
     2401 tcataaatcc ctcattaaca atacgattaa ttttcatccc tgccgcaccc gcgccagggg
     2461 cgttaaggtt tagcgtttcg ctttactgct tcgctgggtc agcaagatat tcgccagcac
     2521 cgccaccacg atgatgacgc cgcgtaccac ctgctggaaa aaggagttaa taccgagcag
     2581 caccagaccg ttaccgatta gcgtaatcac cagcacacca agcaatgtac cgaacaggga
     2641 accgcgaccg ccggaaagtg ccgtaccgcc gaccacgacc gcggcgatga cgtcaaactc
     2701 cagaccgttt gcggcacctg cgttaccgga accgaggcgt gccgccagca aaatgccggt
     2761 caccgccgct aataatcccg aaagggtaaa gataagaatg cgcacccgac gaacgttaat
     2821 accgcacaac tgcgccgccg tggcattacc gccaacggca aaaaccgagc gcccgaaggc
     2881 ggtttttcgg ctaatgaaca caaacagcgc aaacaacacc atcatgatca gcgcggatac
     2941 tggcacaccg agaaattgtc cgcccagcca gtccagcacc tcgttttcgt caatcggcac
     3001 tggcagtgcg ttggtcataa acagccccat tccgcgcagg gcgctccaca gccccagtgt
     3061 ggcaacgaaa cttggtacgt taaacacgcc gcgcagcacc ccggcgagcg ttcccatcag
     3121 cgcgcctaac agcaacacca gcagacaagc aatcgccaac ggaacttcaa attgcagcaa
     3181 aaatgccagg cacaccgaga caaaagccac catcggccca acgctgacat caatttcacc
     3241 ggagataata atcagcgtca tcgcccaggc ggcaatccca atggtggcgg catcgcgcag
     3301 cacgttcatc tggttattca atgagataaa gccaggcgcg ttcagggaga agaccagata
     3361 aagaatggca atcaccacca gcaaaccgat ctcattaata tggcgactga caaattgttt
     3421 gagcgagacg ctcttgccct gcggcaatgg taatgacgaa gcagacatct tcaggttcct
     3481 cggtaaaatt catcagtgca cagacagaat ggcggacatc agctcatcca cattgaccgg
     3541 cgagtgaaac tcctgcgaga acgtgccgtg ctgtaataac aggatgcggt cacacaccag
     3601 cggcaactcc tccacttcac tggagataaa caccacgctt tttccttcgg cagccagctc
     3661 acggacaata cggtaaatct gctgtttggc ttcgatatcg acgccgcgcg ttggctcgtc
     3721 gagcaacaaa atctggctgg cggcatagac ccaacgaccg atcaccactt tttgctgatt
     3781 gccaccagaa agcgtgccga tgggcgtttc gctactagcg gccttgaccg tcatccgctg
     3841 catcacctct tcggtcaggc ggcggatggt ggaccattgc agcactccgt tggcgctgat
     3901 tttttgccga ttggtcagca ctgtattttc gtcaacgccc aaccagggaa tgatccccgc
     3961 ttctttgcgg ttttctggcg tatagccaat gccgcgtttc agcatgtcgc cgtaatcggg
     4021 gcgcgtgatt ttctcgccgt tgataacaat ttcgccctgt tcatacgtct ccagcccaac
     4081 aatcgccttc agcaattcac tgcgccctgc ccccagcagg ccagcaatgc caagcacttc
     4141 gccgcgacgt aacgtaaagc tgatatcctc cagcttgggc ttatggcgta acgcacggac
     4201 ttccagtacg gcctgatcca caatttcctg aggggcaacc ggcgcaatat caacgtgatc
     4261 acgccctagc attagcgaca caatatgatg cgtggaggtg ttttcgagca tcacatcgcc
     4321 cgccacctga ccatcgcgca taacggtggc acaggaggca atgcggcgga tttcttccat
     4381 ccggtggctg acataaatca ccgccacgcc cagtgccgac atctttttca ccgcactgat
     4441 caccagttca acttctgcac tggcaagcga gctggtgggt tcatcaagaa tgaccacgcg
     4501 cggctcgccc ttcatcaccc gtgcaatttc caccagttgc ttttgcgccg ggcttagcgt
     4561 tgaaacaagt tgttcaggac taacgtcaac gcccagcgcc tgtaagcaac gttgggcatc
     4621 ctgcgccatt tgcaggtaat caatcatgcc gttgcggcga ggccattgac caaggcagag
     4681 gttttctgcc accgtcagcc cttccaccag acttaattcc tgataaaccg cacgaacccc
     4741 cagttcagcg gcacggcgag tcaacgtagc ttcgtcacct tccagtcgcg tctcgccaat
     4801 ccagatatca ccgctatccg ggcgttcgct gccggtaagc attcgaatga gagtcgattt
     4861 gcccgcgccg tttttaccta acagcgcacg aacttcgcct ttattgagcg tgaagttaac
     4921 gttatccagc gcaacgacgc cggggtaacg cttatttcct gccaccactt ttgctaccgg
     4981 gactgcctct gttgccgtga acatagggac ctctgcgaat cagcgattag ggcagaccat
     5041 caacgtgcgt tgccagccac tgtttaccat cttccgtttt ggtatagaga tcgattggca
     5101 cctgaatcac tttttcaccg tcggcttgtt tattgataac cttcaatgtt tgcgcgaaaa
     5161 cagcattgcc cattttctta ccggaaatat ccactaccgc tttcagcacc tgattgtttt
     5221 ccagctcctg agcaatttcg gttgtcatat ccgaaccgaa aacagcaatt tttccggcct
     5281 gattttgatt acgtaccgct tttaccgcgc cgagtgtcgc accgcctgac tcccccataa
     5341 tggcgttgag atccggcgtg gagataatca gtttttcacc aacggaaatc gctttattta
     5401 aaacagtccc ttcctgatta gcgacaattt gcgcgccggg aacgcgggat tttaatactt
     5461 cttcaaatcc tttacgtcgc tgcacacaaa cttcaaaggc ttcgcaattg atgacggcaa
     5521 ttttcggctg gtcaatttta ttggcaataa aataatcggc ggcagcgtta cccagttttt
     5581 taccaaattc cagtggatcg ccgaccagat acgccgagac atatttatcg acaccctttt
     5641 gattaataca ggtgttgtag caaatcaccg gaatacccgc ttcactggcg cgacgaacgg
     5701 tacggctact gccattttca gacactgccg ataaaataat ggcatcgaca ttacgcgcca
     5761 cgagggtatc aacaaaggta ctttctttcg aaatatcacc ctgggcgtta gtttcaatta
     5821 actgtacctg tactgaagaa tcttttgccg catcctgaac gccctgacgc actccagcgt
     5881 aatatcccag ggtatcaagg tatattgcac caatggtcat ttctttatcc gcagccctgg
     5941 caaatagtgc gctacctaat agcgtagcca ttaataatag attacgggtt gttctcattt
     6001 ttttaggcat agtgcctcct gtagagtttt tattaacaac ggcttattct aattattttg
     6061 tgatgagcgg cagcgcgtgc cgctcaaata ttacagttaa tagggtttag tgaacgtagt
     6121 taaaaataaa aggcatcacg gtggtgaata atgccgcttt cggccaggcc ggattaatcg
     6181 ccgtcaattc agcgcgagca gattcatatt gcgcaacctc cccgagtccc gcggacgcca
     6241 gcatcgccac catcaggcat ttttctttat gctgctgttg taaatcgcca tacagcgaca
     6301 acaggtcagg ttgtgaaaca gcaaagaaat ccgcctcaat actggttttc gccatctctt
     6361 gcgcccactg tttcatttca ctaaacagtt gctgtgcgat ttgttgttcg cccagcagtc
     6421 gcagcgccat tccttgccag aagagataat caaccggctg atcgttgtaa taactgtgga
     6481 tgttaatggt gcgatcgccg gtcgccgcca gacgtaaaca acgcgtcgct tcagtttcat
     6541 cgccctgcgc gttggcgcat atcgcctgcc agaaccagat gtcgttatca gtttgccccg
     6601 gtaaacggcc ttcgcttaaa ttctccggat aatgcagcgc ggcatgaagc agttcgcagg
     6661 cctgctgcgg ctgtctggca tcaagatgct gccaggcgcg taataactgg ttgaggataa
     6721 actgactggt gaccttccct tccccgcctt cccacgggtg gaatttgcgc gtggcgagaa
     6781 tgtccgccgc tttgtctgcc tgacccgtga gatgccacaa attgagcagt tctgcggtca
     6841 tgtcgtcgcg tttcagcgca atttccagat tattttccag acgcgccagt cgtttctccg
     6901 gtgtggctcc acttaactta tccagcaaat cccgttcgaa aagcagacgt gcatcctgcg
     6961 gcgcaagctg ataagcatta tcaagataac gcgcggccag ttcataatcg tgttgcttat
     7021 tccacgcatg gatcgccaac ccgcgccagc catcggcaaa ctccggcgac atctctacgc
     7081 aacgttgcca taaagtaatg gctttgttgt agctgcgctt gttgtagtag aaacaggcca
     7141 gtaaatgacg ggcaaaccag cactcttcaa tactctccag cgccgccact tcttccagcg
     7201 tattcgggaa acggacaaac tgcgggaaga catcaatggc ttttgcgacc agttcgccgc
     7261 gttcggcttt cggcagcaag ctggcttgta gataaagcga taacgtgcgc tgacagtcca
     7321 gtgcgttcag catctctgtc gccagagtgg gcattcccca gttaatcagt tgcccggcgg
     7381 tcatcagggc gttaacgtcg cgtccctgac acagaccgcg ccactgcgcg agggctgatt
     7441 cgctacgacc atcgaaccag ttcagccacc acagaatggc gttcagcgga taatcacgca
     7501 gcagtttcgc gcgctgcaaa cgcgcgttgt cctgacgacc acttaacacc agcagcagat
     7561 tatgcaggca aagcacttcc tgattggttg ggcaggcgcg aagactttgt tggcaaaaat
     7621 ccagaccagc gtcgaagtta ccattacgcg ccgccagtcg tgccaggcca taatagccac
     7681 cggctttgct gttgccgctc cagaccgcac gccagaaatc ctcttcggct tgttgatatt
     7741 gtccctgacg ttcgtaagca ctggcgcgaa tcaaacttgc ttgtccacac tgcgggtttt
     7801 tgttcagcgc atgtgcgcgt ttcagcgcct gactggcata cgccaccgct tgcgggaaat
     7861 ctgcgcggtt atattccagc atcgccagcg ccaggttaca gcgataatcc agcgggtcca
     7921 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
     7981 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gccagtggcg
     8041 ctttggcgac gtccggtaac ggcaacgctt gcggctgatg ttcctgataa ctcagtacaa
     8101 tattgccgtc ggcatcggaa agctcaatgg tcagcctatc aggattgata ccgtgcaaca
     8161 cgccctggat ggcggtcgca ggcatcagtg ccacggcatc atcaagtaac gcgttgcatt
     8221 tgccgatttc gtggatcgcc aggcggtatc cgttcaacgg agagatggca tacagccccc
     8281 actcaatccc ctgctcacta cgctggagtt ttatcaccgc atcgcgggag gcattttgca
     8341 ccatgcccag agaatgataa ggcaggaaat actgctcaaa acgcttctct tcgtaagcat
     8401 caagccaggt aaaatcgggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
     8461 ggccgttatt gtcggtcaga ctcttatccc acgcctggcc aaattcactg tgtccccaac
     8521 tccactgttt tttacctggc gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt
     8581 catcgtgaca ccacgcgccg acaaaatcgt actgcgattt ttcggccata tatgaggttg
     8641 gaacgggcac atttttatag cgagaaatgt ccactccagc ggagtagtcc actttgtagt
     8701 aagtgccggt agcgatgggg aaagcggaga cggcccgttt gccgtgatca aacaccgcag
     8761 tcaaatccgg tgggaaaacg ctctgatgcc cttcaccccc tttcactgcc gggttggccc
     8821 accacaagaa atgacgcggc gtggcgttgc cgttatagac gcggctggcg atttccagcg
     8881 ccgcccaggg cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc
     8941 gcctacccac accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat
     9001 aaaggtggtc gggcgatgat gttgcggcca gttaaactca atcccaccgg aaatccacgg
     9061 ccccagcagc cccaccagcg caggtttaat gacttcattg tgataaacaa aatcgcgctg
     9121 tttcacttta tcccacgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt
     9181 gatgtagtcg ttttccagcc acaccgcctg ccaggatttc agggttttct gctcgctcag
     9241 cgtatcggtc acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc
     9301 caggaacatg ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc
     9361 ttgccacact tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa
     9421 agatttgtgg cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta
     9481 actaaacaag gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat
     9541 aacaaatgcg tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg
     9601 gcccggctgg cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc
     9661 gaaaaacggg tggtgaatat cgacgatgaa agccagacgc gcgggcatag tagcggtaca
     9721 tggctgattg cgccggaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt
     9781 gagtgtcagg ttgctaatgc ttgtttaagt ccgaaaggtg aatttgagta tttacagatt
     9841 gatgcaccga cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt
     9901 aaattgtggc cggaccgcac catcaatctg gcgctggcaa tccacggtca ggttgatccg
     9961 gtgaccggcg tgtcgcaaac catgccgcaa gcgccgtgga caacgccggt tgaggtgaag
    10021 tatctgctgg aagagaagct cggcattcgg gtgatggtcg ataatgactg cgtgatgctg
    10081 gcgctggcgg agaaatggca aaataattcg cagaaacggg atttctgcgt gatcaacgtt
    10141 gattacggca ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat
    10201 ggtagcggac agattagtca caccatcgtt aatccggatg gcgtcgtctg cgactgtgga
    10261 cgttacggct gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta
    10321 tggctaaaat cacaaccggt taatactcag cttgatccgg aaaaactgac tacagcgcag
    10381 ttaatcgctg cctggtagag tggagaaccg tggatcacca gttgggttga tcgcagtgcc
    10441 aatgccattg gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg
    10501 ttgtacggtc gcagttgtgc ctttggtgag cactggctta ataccattat tcgccagacg
    10561 ggatttaacc cgttcgaccg cgacgaaggg ccgagcgtga aagcaacgca aattggcttt
    10621 gggcaattaa gccgcgcaca acaggtgttg gggattggct atttgtatgt tgaggcgcag
    10681 ttacgacaga tttgatggcg caataacgta gaaaggcttc ccgaaggaag ccttgatgat
    10741 cacataccta agattgcctg atgcgctacg cttatcaggc ctacacggat attgcaatat
    10801 attgaatttg caaagttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    10861 acgagcacat tgacagcaaa tcaccgtttc gcttatgcgt aaaccgggaa acgtgcgcag
    10921 atgtcgagaa ctttaccttt gatgcgctcg ataacggctt catcattgat gctgtccagc
    10981 acgtcacaca tccagccagc cagttctttc gcttctgctt ctttgaagcc gcgacgcgta
    11041 atcgccggag tacccacgcg aataccggag gtcacaaacg ggctcttcgg atcgttcggt
    11101 acgctgtttt tgttgacggt gatgttagca cggcccagag cggcgtctgc ttctttaccg
    11161 gtcaggtttt tatcaaccag atcaaccagg aacaggtggt tatcagtgcc gccggaaacc
    11221 actttgtagc cgcgctcgag gaacacttct accatcgctt tagcgttttt cgcgacctgc
    11281 tgctggtaag ttttgaactc aggctccatc gcttctttca gagcaaccgc tttaccggcg
    11341 attacgtgca tcaacggacc gccctgacca ccagggaaaa cggcagagtt cagttttttg
    11401 tacagctctt cgctaccacc tttcgccagg atcaggccgc cgcgcggacc cgccagggtt
    11461 ttgtgagtgg tggtagtaac aacgtgagca tgaggaaccg ggttcgggta gacgccagca
    11521 gcaaccaggc ccgcaacgtg cgccatatca acgaacaggt aagcaccgat gctgtcagcg
    11581 atttcacgca ttttcgccca gtcaaccacg ccggaatatg cagagaaacc accgataatc
    11641 attttcggct tgtgttcttt ggcttgtttt tccagatcgg cgtagtcgat atgaccggta
    11701 gcatcgatac cgtaaggaac gatgttgtac agtttaccgg agaagttaac cggagaaccg
    11761 tgagtcaggt gaccgccatg cgccaggttc atacccagaa cggtatcacc tggttccagc
    11821 agcgcggtgt agaccgcaaa gttagcctgg gagccggagt gcggctggac gttagcgtag
    11881 tcagcgccga acagttcttt cgcacgatcg atcgccagtt gttcaacgat atcaacatac
    11941 tcgcaaccgc cgtagtagcg tttgcccgga taaccttcag catatttgtt ggtcagctga
    12001 gaaccctgcg cctgcattac gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg
    12061 tgctcttcct gacgtacttt ttcctgctcc atagcctgcc acagttcggc atcataatcg
    12121 gcaatgttca tttcacgctt taacatccgc atctcctgac tcagctaaca ataaaatttt
    12181 tggcctttat aggcggtcct gttggacaac ggcgaacagt ataaccgaat cattgtgcga
    12241 taacaggtct tgacaaagga atttacgcaa acgattacct tcaggctacg caaggctttg
    12301 gagaataaag agtttgcaac cggaaacgga tttcttttca ggtttgtgat gcaaattttt
    12361 cacttcatca cattctttct gaaaaacacc aaagaaccat ttacattgca gggctatttt
    12421 ttataagatg catttgagat acatcaatta agatgcaaaa aaaggaagac catatgcttg
    12481 acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa
    12541 agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt
    12601 ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg
    12661 cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc
    12721 agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt
    12781 tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag
    12841 cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg
    12901 ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc
    12961 gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat
    13021 accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa
    13081 ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatt gcggtgaaac
    13141 gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg
    13201 tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga
    13261 cgttaatctc tgccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa
    13321 aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc gatgttcacg
    13381 cctttgccga tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct
    13441 ggcatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga
    13501 tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctatgcg
    13561 gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg
    13621 aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat
    13681 ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac
    13741 ggatgcgaat gacccgtgcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg
    13801 tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa
    13861 tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg
    13921 tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga
    13981 cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct
    14041 ttttcatgct attccttgaa aaggtcgcct gtcttttgat ctgctaaacg taacacataa
    14101 cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa
    14161 attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt
    14221 gcagcagctt acgcaaatag ttgagttcaa actgattacg tgcctcaaca aaggttggca
    14281 gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg
    14341 aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc
    14401 tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc
    14461 gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg
    14521 ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt
    14581 ccccgcgcgc catcgctttt ggcagatcac ggtgagtggc agaaataatc cgcacattga
    14641 tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca
    14701 gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt
    14761 ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat
    14821 gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa
    14881 atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg
    14941 tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt
    15001 ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg
    15061 tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga
    15121 caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag
    15181 aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa
    15241 atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt
    15301 cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt
    15361 cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata
    15421 aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca
    15481 ctctcgtgcg gcgtatccgg cgagaaattt ccagtcggtt tgcgggtcga gagctggcgt
    15541 tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg
    15601 tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa
    15661 cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac
    15721 agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg
    15781 cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt
    15841 tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga
    15901 tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc
    15961 agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt
    16021 ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat
    16081 gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt
    16141 cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga
    16201 catggacagc gcggagaaac cggagcgtac atatcgtacg tgaagatttc gagcactgcc
    16261 cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt
    16321 ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat
    16381 acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc
    16441 ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc
    16501 gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat
    16561 gtgaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt
    16621 aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga
    16681 gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg
    16741 tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac
    16801 ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcggctgctg ctatcaagaa
    16861 tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctggtca gccagtaatt
    16921 cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa
    16981 attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc
    17041 caaccgagcg taactcgctc ggtccactga acgaaacgct attgcccaga gaacgccctt
    17101 cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga
    17161 aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt
    17221 gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg
    17281 tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag
    17341 catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg
    17401 cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac
    17461 gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac
    17521 ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa
    17581 taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg
    17641 cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt
    17701 gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga
    17761 atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt
    17821 tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc
    17881 tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac
    17941 gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact
    18001 gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat
    18061 ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag
    18121 ataaacttat tagattgcaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt
    18181 aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga
    18241 tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc
    18301 caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc
    18361 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    18421 gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc
    18481 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    18541 gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga
    18601 gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt
    18661 tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg
    18721 ccacaactcg ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca
    18781 tacccccagc gccagcgttt gcggacggtg gaagaaggtt gcaaactcat cgcgtacacg
    18841 gtcattgaac aggattgact tcgcccaacc ttcaccggca cccagcacat caccgtagga
    18901 gaaaccaccg cacgcgacca gggcgtggaa atcttccagg cccgtgcgtc cggtcagcag
    18961 gtcactcata tgcacgtcga tagcatcaaa gcctgcacgg tggaaagctg ccgccatttc
    19021 aacatgcgag ttcacgccct gctcacgcag cacagcaact ttcggacgtg cgccagtggc
    19081 aatatacggt gctgccacat cttcgttgat atcgaacgac agtttcacat tcaggcccgg
    19141 atcggcgtcg tttgatttcg cctggtgctc ctggtcagca cactccgggt tgtcacgcag
    19201 gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtggtgc ggctttcgct
    19261 gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgccctac
    19321 ataatggaca caatcagcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc
    19381 agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc
    19441 gtcgcccaga gaggcaatat ccgcgttaat gccgcaatga ccagcaaagg ccatttccgc
    19501 cagcgttacc agcaggccgc catcagagcg gtcgtggtat gcgagcagct tacgctgtgc
    19561 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgc gcgacgtcgc gtacatctgc
    19621 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    19681 gcctttgccc aaatcaatca gcagcagtgt gttatcttcg gtagaaagct gtggcgtgat
    19741 ggtgtgacgc acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt
    19801 catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc
    19861 caccgggatc gtcaggccca gcgccggaca aagctcttcg cccacggctt taacggcttc
    19921 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    19981 aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc
    20041 cagacgggca gaggcggcga aatccagcag tgcaaccggc gcacgctcgc caatcgccat
    20101 cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    20161 ctgccacggc cccaccatct gatcgcgcgc taccatgccg gttacgctgc ggtcgccgat
    20221 ggtcaccagg aaggttttct ctgccacggt cggcagatgc agtacgcgtt tcaccgcgtc
    20281 tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc
    20341 gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt
    20401 atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    20461 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac
    20521 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg
    20581 ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgcccgccgt cgctcaccag
    20641 ttccggcatc gcgttagaaa gaccgccagc gccgacgtcg tggataaaca ggattgggtt
    20701 ggcatcacca agctgccagc aacggtcgat cacttcctgg cagcgacgct ccatctccgg
    20761 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgacctg acgccatgga
    20821 agacgccgct ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    20881 cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc
    20941 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccgttgtggc tgttcacttt
    21001 ttcttcataa gtacggaagt agccattcag tgccggacga ccaaattcgt tgttaaacgc
    21061 cgcgccgccc agcgggcctt cggtcatgat gtccagcgcg gtgacaatgc gctcaggctt
    21121 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg aaacggagaa
    21181 accaaccaga ccagccttcg gcttcgcacc gcgtccggtg gcgccttcat cacgaatttc
    21241 gccgccggaa cccgtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    21301 ttcgactttc atcagaatat gcgccggttc ctgatggaaa tcgtagcggc ccgtttcgtg
    21361 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    21421 agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcttga acagcgattt
    21481 cggctgctgt tcaccgtcga taatccagtc ggcattaaaa attttgtggc ggcagtgctc
    21541 agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt
    21601 gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt
    21661 agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgctggtaa ccggagtcgg
    21721 ttgatggtgg gcaaacaact gctctgcatc atctaaagca aaaaagaccg tttccatcat
    21781 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    21841 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc
    21901 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    21961 gagtttgcct tgcggggcgt ggctggcgag tgccgggcca tatttcagca ggcgttcaag
    22021 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    22081 gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    22141 gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    22201 gctttcggtg tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac
    22261 gaaaccgttt gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg
    22321 ctgtaataag ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac
    22381 ctttcactat tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat
    22441 tgaaaaaatt aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg
    22501 ctctctggcc atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag
    22561 cgcggggaga gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg
    22621 ggaaaccttt tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggtgtaa
    22681 aactgaaagt gaccgtgcgg cagaatatca gccagctgtt tgacgacctc gataatggta
    22741 acgccgacct gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc
    22801 ctggccctac ctattattcc gtgtcacaac aactggttta taaagtgagt cagtatcgcc
    22861 cgcgcacact gggcaacctg acggcggagc aactcaccgt tgcaccgggt cacgtggtgg
    22921 ttaacgatct ccaaaccctg aaagacacaa aattcccgga attaagctgg aaggtagacg
    22981 acaaaaaagg ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca
    23041 ttgctgattc tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc
    23101 tcgatatcac cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc
    23161 tttccgccgc cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca
    23221 ttgaagagaa atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt
    23281 tacgcgccgt cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag
    23341 aaattgactg gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg
    23401 ccacttcacc gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc
    23461 tcggcattac ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc
    23521 aggatatgat gagtaaagtg ccggaaagtg tgccggagaa cgagcggatc tggtttgccc
    23581 tcgctgcgta caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa
    23641 ccaaagggaa tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga
    23701 aaccctatta cagcaagctg acttacggct acgctcgtgg acatgaagcc tacgcttatg
    23761 tcgaaaatat tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc
    23821 aggctacaga agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt
    23881 tgggcaaaga gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga
    23941 cccattctcc ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg
    24001 tcgagggttg cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac
    24061 tgagcaacgc cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca
    24121 tacccggatg atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg
    24181 caccaaagac cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg
    24241 gttcaagcgt gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac
    24301 cctgccgcag ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt
    24361 tccagccttc gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc
    24421 gctcatccca ggcacgtttc gccagcgtca gcgcgtgacg catccagtat tcgtggctaa
    24481 attcgacttc agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac
    24541 gctatgcttt actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat
    24601 gctgacagaa ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc
    24661 gcagcggagt gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca
    24721 tcgtcaatac ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg
    24781 gcagccaggg cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca
    24841 accagatatc agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa
    24901 aggcggtaac attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg
    24961 cttcgctgtg accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac
    25021 gaccttttat caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat
    25081 tcagcggttg gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca
    25141 atgttccatc taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct
    25201 ctggactgaa atatcctgat taattttcgc tactacatag cctaacagat agatcatcac
    25261 ttttccggca acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag
    25321 cggcaatgga atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt
    25381 tcatcgcgag gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg
    25441 cccagagtga taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt
    25501 taagctctca gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg
    25561 cgcaaaaact cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg
    25621 caagccagcc ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt
    25681 tacggctggt cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa
    25741 acgttaatag tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga
    25801 taattctgac cggtattggc gcttcgggtc tggtggcgca aaactttgcc tggaagctga
    25861 tgaagattgg cttcaatgct gccgcagtgc gcgatatgca tgcgctactc gcaacagtac
    25921 aggcgtcgtc ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt
    25981 taaacctggc ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct
    26041 ttactccgaa tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac
    26101 aagcgacaaa cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc
    26161 tgttcattgc gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg
    26221 aagcgctggt gaaaaaactg gtctgagtaa agaatgcgcg tacaatgccc gcccggtttg
    26281 tgttgttttg agagtttcct tatggcgttg ttaatcacta aaaaatgcat caattgtgat
    26341 atgtgtgaaa tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga
    26401 atgtgttgtt ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca
    26461 ccactttctt taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg
    26521 tgacatacca cattaacttt tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc
    26581 ggttttatat aaccctggct ggaaacctct tatacaaaaa agtcgattcg ccaacatcat
    26641 agatgatcgc caccttctgg cgagaaactc ctgatgcaat tagtcgcccg gcctgcgccc
    26701 attattctgg tgaaagtttg ggacggcgac cgccaattcg tccctatgcg cgagcagctt
    26761 ccagtcctgc ttttgttcgt tcaacaatca gttcacgttc catttcagcc agggcaccca
    26821 tcacatggaa gaaaaagcgt cccattggtg tgctggtatc aattgaatca gtcagactac
    26881 gaaagttgat gcctcgttcg cgcaactcct ccaccagcac gacaagatgc cgcatactgc
    26941 gccccagccg atccagcttc cagacaacca gtgtgtcgcc tgccgataat gtcctgagca
    27001 gttttttcag ccccggcctt tcggactttg tgccacttat cttgtcttca aaaatcagct
    27061 cgcatcctgc acagttcaac gcattacgtt gtagatcggt attctggtca tttgttgata
    27121 cgcgtacata gccaataagc atggtaaatc cc
//
LOCUS       DAZSBF010000034        27104 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZSBF010000034 DAZSBF010000000
VERSION     DAZSBF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.034"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..921
                     /locus_tag="ACHXWD_001112"
     CDS             <1..921
                     /locus_tag="ACHXWD_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7620085.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHXWD_001113"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="ACHXWD_001113"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR7620086.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHXWD_001114"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="ACHXWD_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR7620087.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="ACHXWD_001115"
     CDS             3882..4277
                     /locus_tag="ACHXWD_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR7620088.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="ACHXWD_001116"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="ACHXWD_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR7620089.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="dolP"
                     /locus_tag="ACHXWD_001117"
     CDS             4897..5472
                     /gene="dolP"
                     /locus_tag="ACHXWD_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR7620090.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="ACHXWD_001118"
     CDS             complement(5586..6626)
                     /locus_tag="ACHXWD_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR7620091.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="ACHXWD_001119"
     CDS             complement(6699..7334)
                     /locus_tag="ACHXWD_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR7620092.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHXWD_001120"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="ACHXWD_001120"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR7620093.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="ACHXWD_001121"
     CDS             complement(7960..8403)
                     /locus_tag="ACHXWD_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR7620094.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHXWD_001122"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="ACHXWD_001122"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR7620095.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="ACHXWD_001123"
     CDS             complement(8743..9246)
                     /locus_tag="ACHXWD_001123"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7620096.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="ACHXWD_001124"
     CDS             complement(9240..9764)
                     /locus_tag="ACHXWD_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR7620097.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="ACHXWD_001125"
     CDS             9973..10968
                     /locus_tag="ACHXWD_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR7620098.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="ACHXWD_001126"
     CDS             10977..11855
                     /locus_tag="ACHXWD_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR7620099.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="ACHXWD_001127"
     CDS             12061..13068
                     /locus_tag="ACHXWD_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR7620100.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHXWD_001128"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="ACHXWD_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR7620101.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHXWD_001129"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="ACHXWD_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR7620102.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHXWD_001130"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="ACHXWD_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR7620103.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHXWD_001131"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="ACHXWD_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR7620104.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHXWD_001132"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="ACHXWD_001132"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR7620105.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHXWD_001133"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="ACHXWD_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR7620106.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHXWD_001134"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="ACHXWD_001134"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR7620107.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHXWD_001135"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="ACHXWD_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR7620108.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHXWD_001136"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="ACHXWD_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR7620109.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHXWD_001137"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="ACHXWD_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR7620110.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHXWD_001138"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="ACHXWD_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR7620111.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="ACHXWD_001139"
     tRNA            complement(26826..26902)
                     /locus_tag="ACHXWD_001139"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6420 a         7516 c         6811 g         6357 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccaag
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ctta
//
LOCUS       DAZSBF010000035        26863 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZSBF010000035 DAZSBF010000000
VERSION     DAZSBF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.035"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHXWD_001140"
     CDS             complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHXWD_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR7620112.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHXWD_001141"
     CDS             complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHXWD_001141"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR7620113.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2203..3618)
                     /locus_tag="ACHXWD_001142"
     CDS             complement(2203..3618)
                     /locus_tag="ACHXWD_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7620114.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHXWD_001143"
     CDS             complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHXWD_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR7620115.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHXWD_001144"
     CDS             complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHXWD_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR7620116.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHXWD_001145"
     CDS             complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHXWD_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR7620117.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11339..11395
                     /locus_tag="ACHXWD_001146"
     CDS             11339..11395
                     /locus_tag="ACHXWD_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7620118.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11666..11722
                     /locus_tag="ACHXWD_001147"
     CDS             11666..11722
                     /locus_tag="ACHXWD_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7620119.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHXWD_001148"
     CDS             11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHXWD_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR7620120.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12275..12934
                     /locus_tag="ACHXWD_001149"
     CDS             12275..12934
                     /locus_tag="ACHXWD_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR7620121.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12931..13692
                     /gene="pphC"
                     /locus_tag="ACHXWD_001150"
     CDS             12931..13692
                     /gene="pphC"
                     /locus_tag="ACHXWD_001150"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR7620122.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            13689..15629
                     /gene="yegI"
                     /locus_tag="ACHXWD_001151"
     CDS             13689..15629
                     /gene="yegI"
                     /locus_tag="ACHXWD_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR7620123.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHXWD_001152"
     CDS             complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHXWD_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7620124.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            17128..17976
                     /gene="alkA"
                     /locus_tag="ACHXWD_001153"
     CDS             17128..17976
                     /gene="alkA"
                     /locus_tag="ACHXWD_001153"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR7620125.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(18086..21403)
                     /locus_tag="ACHXWD_001154"
     CDS             complement(18086..21403)
                     /locus_tag="ACHXWD_001154"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR7620126.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            21721..22362
                     /gene="udk"
                     /locus_tag="ACHXWD_001155"
     CDS             21721..22362
                     /gene="udk"
                     /locus_tag="ACHXWD_001155"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR7620127.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            22454..23035
                     /gene="dcd"
                     /locus_tag="ACHXWD_001156"
     CDS             22454..23035
                     /gene="dcd"
                     /locus_tag="ACHXWD_001156"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR7620128.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            23057..24910
                     /gene="asmA"
                     /locus_tag="ACHXWD_001157"
     CDS             23057..24910
                     /gene="asmA"
                     /locus_tag="ACHXWD_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR7620129.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(25184..26767)
                     /locus_tag="ACHXWD_001158"
     CDS             complement(25184..26767)
                     /locus_tag="ACHXWD_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR7620130.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         6370 a         7187 c         7224 g         6082 t
ORIGIN      
        1 tcagtttgct gaccatgcgt gggataatgt tgccggatct ggtctgaacg ccatctccgg
       61 ctaacaaagt catgtcgcta aaactaaacg atgcggcagg cgtcggcttc ccagcggtaa
      121 ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc cagcttgcgg
      181 cgcaggtttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta gtcgtcataa
      241 agatgattga gcaattgctc gcgggagaac acttttcctg gttcatgaga gagcgttttc
      301 aacagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga agcctgaaaa
      361 cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgcaattc gcgctgcggt
      421 ttgcaacggc gcaaaatggt tttgacgcgc gctaccactt cccgtgggct gtacggcttg
      481 cagatgtagt catctgcgcc aatctccagt ccgagcaggc ggtcgatctc ttcgattttt
      541 gccgtcacca tcacgatagg aatgtcagaa aaacgacgaa tttcccggca cagcgtcaag
      601 ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt ctggcgcaca
      661 tacggcagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc agcacgcaga
      721 taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat acgcggtgtg
      781 ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc agcggtaact
      841 ctactgtaat gcttaccccg ccaaaaggcg aatgggcagc aataatgcgg ccattatgtg
      901 cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accaccgctg gcgcggttgc
      961 gggaaccttc ggtgcgataa aaacgttcaa ataatttttg tagctgctcg tcactgacac
     1021 ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg gcagagattt
     1081 tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc aagtaaatta ttgaataact
     1141 gcattaaacg gtcgcgatcg ccaaatacgg taatactgtc tggcagggaa aattgcagtt
     1201 tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc agcagtggga
     1261 tcaaatctac cggtgctttt tgataggcga gggcgccttc atcagacatc gacaactgat
     1321 ggagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac gccaccgtct
     1381 ccggcgtgaa tttgcgcacg ccatcctgaa tagcttccag ttcaccgcgc agcaccgcta
     1441 atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt tgctggtttt
     1501 tctccagtgt gctggcaagc tggttgaagt cttgcgccag tttgcccagt tcatcttcac
     1561 tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg ccatcgacaa
     1621 gtcgttttac cggtgccagt aaaccgcgcg ccagcagaaa agtggcaagt gccgcgagta
     1681 acgttgccag ggcgacaatc aaccagctgg tttgccgctg ttgtttatcg aaattgatat
     1741 cagtattgcg cgttaaccgc tcaacggggg aggcgatcac cgcgccaact tccgcaccgt
     1801 tgaccagaat gggtcgccgt gtaccgtcag gtggaatcgg cgctcgcgga ccaaccagca
     1861 ctttgttgtt ttgatcaacc acccagaact gggtacgcca gccgtgcggt ggcatacccg
     1921 ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga aagacaaagc
     1981 gatcattgtt gcgcaggaag cgccagttgc catgctgcgc atactgctcg ccaagcgcat
     2041 cacttaacag ttgtaatcgc tgttcattac catgcttgat gtaatcaata aagccacgct
     2101 caaaactgat acgcaccgcc cagtgcatac tgatcagcaa aacaatgcag gtggcgaaaa
     2161 tcgccagaaa cagtttgccg gtaataccag gtcgccagaa cttcattgcg cgctcctttt
     2221 tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa agatgaacgc
     2281 cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg tttgtgtggt
     2341 gccgctgtcg acgctgacat gctgcgaacc aaaaagtccc agcaacagcc cggcgatggt
     2401 gacgccgata ctcatcgaca attgcataat catcgacagc agactgttgc cgctgctcgc
     2461 cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac gggtcgagtt
     2521 gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca acagagcggt
     2581 agtcataaac aacagggtga tcagcgacag acccagcgtg gtcgctacca gtacccgacg
     2641 ataaccaaag cgattcacca cctgaaccac aattcgcttc attcccatgc tgccaagtac
     2701 catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa tttgcaggaa
     2761 aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc tccccgccag
     2821 gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc ggttgttatt
     2881 tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca ggccagcaat
     2941 cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta atacagccat
     3001 gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca tggtgtagtt
     3061 cggcattaac agcaatgtgg cgatcgcacc gataatcccc accggaatgt tgatcaaaaa
     3121 gatccagtgc cacgacgcgt actccaccag cagaccgccg agcgccggac cgagcagcgg
     3181 accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct cgcgcggtac
     3241 gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa cgccctgtaa
     3301 cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca gtgaaccgag
     3361 agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt ccgccagcca
     3421 gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga ccatgtgcat
     3481 atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt taacgatggt
     3541 ggtgtccagc gactgcataa agaagccgaa agccacaatc cacaattgcc agcgggtgct
     3601 gtcgggaagt tctgtcattt actcggttac cgtttgttta ggtttacgcg aaaaacgcag
     3661 ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca gaagctggct
     3721 cattaccagt ccaccgacaa tggtgatccc caggaggtgc cgcagctccg agccgtcacc
     3781 gcccgacaac accagcggca gcgcgccaaa cagcgccgcc agggtagtca tcataatcgg
     3841 gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt taccgtgccg
     3901 ttgggcttcg agcgcaaaat cgaccatcat aatggcgttt ttctttacga tgccgattaa
     3961 tagcatgatc cctatcaggg ctattaggct gaacggggca ttgaacagct ccagcgccag
     4021 cagcgcacca acgcccgccg agggcagggt ggagagaatc gtcagtgggt gtacgtaact
     4081 ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa tcaggatcac
     4141 ctgtgagttc atcgtctcct ggaacacctg cgctgttccg gcaaaactgc cgcgcaccgt
     4201 cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt ccgagagcga
     4261 ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct gatgattcac
     4321 cgacagcgga gcgttcgccg gttgccattt agcgaaatac gacagcggga tcgctttgcc
     4381 ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat agcgcggatc
     4441 cacttccatc accactttat actggttcat cggctggtaa atggtcgaga tttgccgctg
     4501 accgaaggcg ttatttaaca ggctgttggc ggcctgcacg tcgattccca gccgtgccat
     4561 ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat cggagttcac
     4621 gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc attcgcgcag
     4681 tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact gacgcccacc
     4741 aacgcgaata tcctgtaccg ccatcaggaa cagattcgcc cccggttctt tcgccagttt
     4801 tacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc gtggcttgag
     4861 ggtgataaac atcatcccgc tgttcactcg cgaaccgcct gtaaagccgg tgacattatc
     4921 cactgccgga tcgtcacgga taattttcat gaaatcctgc aacttaccgc gcatcgcctg
     4981 aaacgaaata ctctgatccg cctgaatccc gcccatcaac acgccagtgt cctgctccgg
     5041 gaagaaggtt ttcgggatcg agatatacag ccagatattc agcgcaatgg tgccaagcag
     5101 caccacgccc accagacggg tatgattgag cacccatttc agtgacttgc cgtagccttg
     5161 ttgcagggct accaacatgc gaccaaaacc acgcagtcgc ttttgctcgc gcggcttgct
     5221 ggctttcagc atccagccac acatcattgg cgttaatgtc agagaaacca gcaacgaaat
     5281 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5341 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5401 accgacttcg cgagtacctt gcagtgcggc ttgcaacggt ttcattcccg cttccagatg
     5461 acgtgcaatg ttttccagca ccacgatggc gtcatccacc acgaaaccag tagcgatggt
     5521 gagcgccatt aacgaaaggt tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5581 accaatcagc gaaaccggca ccgaaacggc gggaataata gtggcgcgac ccgagcgcag
     5641 gaataaaaac accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5701 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5761 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
     5821 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5881 ggcgttgcgc acatcctgca ctgagtcggt caccgtcgcc acatcgccca gacgaaccgc
     5941 gccgccgttg ttgtagtgaa taatcaacgg ctgatattca gcggcggttt ttagctcatc
     6001 attggtctgg atctgccagc ggtgagtgcc atcttccagc gcgccctgcg gtttacgcac
     6061 gttggcattg ctgatggcgg tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     6121 ctgcggattc agcccgacgc gtacggcggg cagtgagctg cctccgacat cgacatcacc
     6181 aacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     6241 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6301 cgctttgcga taggtcggac ggctgggcat cccactgggc agcaaacttt gtgcagcgtt
     6361 gatcgccgcc tgcacatcac gcgctgcgcc gttgatgtcc cggtcaaaat caaactgtaa
     6421 aataatacgt gtgctgccga gcgaactgct ggaggtcatt tcgctgaccc cggcaatgcg
     6481 cccaagcgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
     6541 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6601 cagcatacgg aagcccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6661 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6721 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6781 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6841 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6901 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6961 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     7021 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     7081 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     7141 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     7201 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     7261 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatctc
     7321 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     7381 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgttcaactt tggcaattga
     7441 gcttaacggc accacgccgc cgtcgctact ggtcaggcga atggtatcca gcgccgcgag
     7501 gcccggggta atttcggtgt tgtgctccag cactacgcga tactggttag cctgagtata
     7561 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     7621 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttg
     7681 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7741 ctgtggcacc caggtactga gcgcatccag tgaggtggcc tgcaaggtaa actggtactg
     7801 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     7861 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7921 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7981 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     8041 cgcgacctgg cgttgtcgct gggccatatt ggtaaaggag ctggattgcg gtgcctgcaa
     8101 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     8161 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     8221 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     8281 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8341 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8401 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8461 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8521 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8581 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8641 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8701 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8761 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8821 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8881 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8941 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     9001 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     9061 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgaccgcacg
     9121 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     9181 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     9241 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9301 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9361 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9421 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9481 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9541 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9601 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9661 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9721 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9781 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9841 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9901 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9961 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
    10021 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
    10081 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
    10141 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
    10201 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
    10261 ttcgaattgg tgcgatccca ggcttctacc accagcgttt ttccggcttt ttgcgcctgc
    10321 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10381 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
    10441 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10501 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10561 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10621 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10681 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10741 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10801 gtaacagtat tagcggcgat aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
    10861 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
    10921 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
    10981 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacccaacgg
    11041 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    11101 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tgtcgggaat
    11161 cgtctgaatg acgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    11221 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccgc cgatttcagc
    11281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat
    11341 gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt actaaggcta
    11401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    11461 tgtcaatctg caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt
    11521 acgcaataat cacactggaa actattttat ccgcaagtca gcgatagact gcatacaggg
    11581 cgaaaggagg taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg
    11641 aaggggcctt aataaggaaa gggtcatgat gaagctactc atcatcgtgg tgctcttagt
    11701 cataagcttc cccgcttact aagactacca gggcggggga aaccccgctc taccctcact
    11761 cctgaaatta tgccttcacg ataagattgt caatccgcag gctttgtagt ctgcgatcct
    11821 gccagcaaat attctttgcg agtcgttacg caataatcac agatgaaact attttgttca
    11881 cgcgttagcg atagactgca ttcagggcga aaggaggtaa gccgacgatt tcagcgggac
    11941 gctgaaacgg gaaaccctct cccggagaag agggctttta ataaggaaag ggttatgatg
    12001 aagcacgtca tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac
    12061 cagggggagg gggaaacctc tccctaaccc tcactcctga aattatgtca ctccgccctt
    12121 agtgtcaatc gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgacg
    12181 ggcctaattc tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac
    12241 cggacctcga taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac
    12301 cagcgatttt gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc
    12361 tggctcgatg agcggcagac ctattaatga acttaacgcc ggattggtta cctttcgtga
    12421 tgaactgctt gctgatccgc tggctttaaa aagagtggaa ctcggcattg tgacgttcgg
    12481 cccggtgcat gtggaacaac cctttaccag cgccgctaac tttttcccgc ccatcctgtt
    12541 tgcccagggg gatacgccta tgggggccgc cattaccaaa gccctggata tggttgaaga
    12601 gaggaaacgt gagtaccgcg caaatggtat ttcgtattat cgcccgtgga ttttcctgat
    12661 caccgatggc gctccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga
    12721 agaagataag aagtttgcct tcttttccat cggtgttcag ggggcggata tgaagactct
    12781 ggcacaaatc agcgttcgtc agcctttgcc actgcaggga ttacaattcc gtgaactgtt
    12841 tagctggtta tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt
    12901 gctggaagcg ccaaaaggct ggacgtcagt gtgagctggc gtctggttta tgcatccgct
    12961 gttggaacat cgcacattag tgctgatttg ccttgtcagg atgcctgcca gatgcaggtt
    13021 gtctggttaa acgaccagca accattactg gtgatgttcc ttgctgatgg cgcaggtagc
    13081 gtttcgcagg gaggagaagg cgcgacgctc gccgtcaatg aagcgatggc gtttatggtg
    13141 caaaaagtac aggacggtga attggggctt aatgatgtcc tcgccacaaa tatggtactg
    13201 actattcgtc aacgactttt tgccgaggca gaagccaaag cattagcagt tcgcgatttt
    13261 gcctgcacgt ttctgggatt gatttcatcg cctgacggca ccttgattat gcagattggc
    13321 gatggcggcg tggtggtaga tttgggtcac ggtctgcaat tgcctctcac gccgatggtg
    13381 ggtgaatatg ccaacatgac gcacttcatt accgacgaag atgccgtttc cagactggaa
    13441 actttcacca gcactgggcg tgcgcataaa gttgcagcat ttacggatgg tattcagcgg
    13501 ctggcgttaa atatgctgga taattctcct catgtgcctt tctttacccc gtttttcaat
    13561 ggactggcgt cagcaacgca ggaacaactc gatttattac ctgaattgtt aaagcagttt
    13621 ttgtccagcc cggcggtaaa cgagcgtacg gatgatgata aaacgttggc actggcgctg
    13681 tggttaccat gaaacccact ttatatactg ctactggtga gtgcgttacg ccaggccgtg
    13741 aactgggcaa aggcggtgaa ggcgcggttt atgatatcaa tgagtttgtc gatagcgtcg
    13801 ccaagattta tcacacgccg ccacccgcct taaaacagga caaacttgcc tttatggctg
    13861 cgacagctga cgcgcaattg ttgaattatg tcgcctggcc gcaggcaacg cttcacggtg
    13921 gacgaggcgg aaaagttatc ggttttatga tgccaaaagt ttctggtaaa gaaccgattc
    13981 atatgatcta tagcccggca catcgtcgtc aacgttaccc tcattgtgcg tgggattttc
    14041 tactctatgt tgcgcgcaat attgcttcat cttttgctac ggttcacgag cacgggcacg
    14101 tcgtgggtga cgtaaaccag aacagtttta tggtaggtcg cgacagcaaa gtggtgttga
    14161 ttgatagcga ctcctttcag attaacgcca atggcacact gcatttatgc gaagtcggcg
    14221 tgtcgcattt tacgccgcca gagctacaaa ccttgtcgtc atttgtcggc tttgaacgca
    14281 cagaaaatca cgataatttt ggccttgcgt tgctgatttt tcacgtcttg tttggtggcc
    14341 ggcatcctta ttctggtgtg ccgcttatct ctgatgcggg taatgcgctg gagacggata
    14401 ttacccattt ccgttatgcc tacgcgtcag ataatcagcg acgtggttta aaaccgccgc
    14461 cacgatcgat tccgctatcg atgttaccga gtgatgttga agccatgttt cagcaggcgt
    14521 ttacggaaag tggcgtggca accgggcgtc cgacggctaa agcgtgggta tcagcactgg
    14581 attctctacg ccaacagtta aagaaatgta ccgtttcggc aatgcatgtt tatcccggtc
    14641 atttggctga ctgcccatgg tgtgcgctgg ataatcaagg tgttatctat tttattgatc
    14701 tcggcgaaga ggtcattacc accggcgggg attttgtgct ggcaaaagtc tgggcgatgg
    14761 tgatggcgtc agtagcaccg ccagcattgc aactgccatt acccgatcat ttccaaccga
    14821 ctggcaggcc gcttccttta ggcctgttac ggcgcgaata catcattctg cttgagatcg
    14881 cactgtcagc attatcgctg ctgctttgcg gccttcaggc agaaccgcgt tatattattt
    14941 tggttcctgt gctggcggct atctggatta ttggcagttt gacaagcaaa gcgtacaaag
    15001 cagaagttca gcaacgccgt gaggcattta atcgtgcgaa aatggactat gaccatttag
    15061 tccgccagat ccagcaggta ggcggcctgg aaggttttat cgccaaacgg acgatgctcg
    15121 aaaaaatgaa ggacgaaatt ctcgggttac cggaagaaga gaaacgcgct ctggcagcac
    15181 ttcatgacac tgcaagggaa cggcagaagc agaagtttct ggagggattt tttattgatg
    15241 ttgcctctat tcccggcgtt ggccctgcgc gtaaagcggc gttacggtct tttggtattg
    15301 aaacagccgc agatgttacc cgtcgtggcg ttaagcaagt gaaagggttt ggcgaccatc
    15361 tgacccaggc ggttatcgac tggaaagcga actgtgaacg ccgttttgtg ttcaggccga
    15421 atgaagcgat aacgccagca gacagacaag cggtaatggc gaaaatgacc gccaaacgac
    15481 atcggctgga atcggcgttg acagtcggcg cgacagagtt gcagcgattc cgccttcatg
    15541 ctccagcacg gaccatgccg ttgatggaac cgttacgtca ggcagcagaa aaactggctc
    15601 aggcgcaggc agatttaagc cgctgctgag ccatttttca attaacgaaa caccacttcc
    15661 gcccagcgtg ccagcccggc ggtgacggag ccaaagtcat cgccgcctgc aatcggaatg
    15721 cccggcaact gttctgccag cgctttttta atctgcggag aacgggcgct accgccggtc
    15781 aggtagataa cgtccggttt ttcctgggcg ttatccaggg ccagttgcac ctgttccaga
    15841 atccgcgcca gtggctggct gagggcgctt tccagccctc gctggctaat cagcgtagcc
    15901 agttcattgc tgataaacgg cagtgaggcg cgggtttccg ctacgcttga aagagcaatt
    15961 ttgcactctt ctgcgctacg caccaggcga tagcttaaac gctgacgcca gactttctgt
    16021 aacagggcca ctttttccgg ttcgcgggca tcgcgtacca gatcgttaag cagacgaccg
    16081 ttggcactac tgtagaaatc actctgcgca ggtacgtcgt tgatggcaac cgcattccac
    16141 cacggcagga tcggcagggc gatgcctttt tcggtttcgc cacccatacc cagcagtggc
    16201 atcaggtttt taaacgccag ggcgatatcc agatcgttac cgccaatacg acaaccactg
    16261 tgacccagca gactggcttc acgatcgaga cgcgaacgcc actgcggccc catcagcagc
    16321 aatgaacagt cagtcgtacc accgccgata tccaccacca gcacccgttt ttcttcctgc
    16381 aaggtggctt cgtaatccag cccagccgcg atcggctcgt actggaatac cacgtccctg
    16441 aatccggcac gcttcgccgc gcgttccaga atctcttgcg cctgggcgtt tgcttcatcg
    16501 ccgcccagcc cctggaagtt gatcgggcga ccaatcaccg cctgagtaat cgcttctggc
    16561 agctgcgcct gcgcctgctg gcgaatgtgc aacatcattg cgcagaccag atcctcaaac
    16621 agcgctacct gctgcggttt taagccgctg gcaccgagga acgattttgg tgatttcaca
    16681 aaccacactt cttctggatc atcaatgtac tgtgccagtg aggaaagacc gaactgcacg
    16741 cttttcgccg taacatcgat atcttcttcg cggttataac gaatcgcccg acgtaacagc
    16801 gcctgcgttt catcgtcgtc tgccggaaca tcatgatggc ggtacagcca ttcgcttacc
    16861 gattcacgcg ttggcgcgca aagcattgaa ggcagcagcg tgctgtcgtt ttccattttc
    16921 agtaattgcg gtttaccgtc acgcatgacc gccactgaac agtttgctgt accgtaatca
    16981 aaaccaataa acacgaatta atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa
    17041 tgttgctgtc gcgacaaccg gaatatgaaa gcaaagcgta acgtctgaat aacgtttatg
    17101 ctgaaagcgg attaacaagg agatgcgatg tataccctga actggcagcc gccgtatgac
    17161 tggtcgtgga tgttgggatt tctcgccgcc cgtgcggtga gcggtgtgga aacggtcgcg
    17221 gacaattatt atgcccgtag tctggcggtg ggcgaatatc gcggcgtagt gactgctatt
    17281 ccggatatag cccgccatac tctgcacata aatttaagtg caggtttaga acctgttgcc
    17341 gcagagtgtc tggcgaaaat gagccgcctg cttgatctgc aatgtaaccc gcagattgtt
    17401 aacggtgcgt tgggaaagtt aggcgcgggg cggcccggat tgcgtttacc cggctgtatt
    17461 gatgcttttg agcagggtgt gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg
    17521 gcaaaattga ctgccaaagt ggtacagctt tatggcgaac ggctggatga ttttccggag
    17581 tatatctgct tccccacgcc tcagcggctg gcagcagccg acccgcaggc attaaaagcg
    17641 ttaggcatgc cgttgaaacg ggcagaggca ctgattcatc tggcaaatgc ggcgctggag
    17701 ggcagcttac caatgacaat tccgggcgat gtggagcagg cgatgaaaac gctgcaaact
    17761 tttccgggta tcgggcgctg gacggcgaat tattttgctt tgcgtggctg gcaggcgaaa
    17821 gatgtttttc tgccggatga ttatctgatt aaacggcgat ttccgggaat gacaccggca
    17881 caaatccgcc gttatgccga gcgctggaag ccctggcgtt cttatgcgct gttgcatatc
    17941 tggtatacgg aaggctggca accagacgaa gcatgacgtt ggtcgccgga tgcggtgcga
    18001 gcgccttatc cggtctgggg aatgagcgat ttgtaggcat gataagacgc gttagcgtcg
    18061 catcaggcaa tgggcaaacg ctctctcagt taatcgcgaa ataactgcta ttcaccagca
    18121 aatccagcgg ttgggcatcg gcaatcacat caccataaat cagatcgacg ccgatgccag
    18181 aaagcgtatc catcactaag ggtaaaacga ctggcccggc gatggttttc atcccgagtc
    18241 gctgagcgtg cccctgaata atcgtaatca gcatctcatc catcaaatta ccctgcacgt
    18301 tggcgcataa ctcaccatca agtagcaggt aatctgccat attcgctttc agcgagttga
    18361 agatttgcag atcgcggccc acctggctga gcactatccg acatcccgcc agctgcagtt
    18421 tttgcacgct ttctgcgtga tctaaaatcg cttcagccgg aataatcaga tgtaataacc
    18481 gtggtggtag agggctattt tccagctgct caagcagatc attcaccagc gtggcgctac
    18541 tcaaaccggc aacggaaagg gggagggcga tgcttatgcc tttactggca accgctttgg
    18601 cggcctgctg gaaaaattcg tggaataccc gtcggtcaag agcatggcta agtgccggat
    18661 cgctgaagct acgacgaaat gtttgttcat caataatttc gccttcgcaa ctccagagct
    18721 taagcgaaat cagccacaaa ttacgcgctt ccgggatccg cggcgaagcg acaccgtggg
    18781 cgatcatcat caactgattc tctttaatca tccgccactg ttcatcaagc gacatcgccg
    18841 cccgttcgct atgtgcggca gcttgctgtg gttcgtaaac cgtcacccgg ccccgtccac
    18901 catttttgga ggcataacag gcgatatcag cctgcgacat cacttctgcc gcctgatgat
    18961 tgttgtcatc gatcaaggta atcccggcgc tggcacctac ccgatgtacc cggccttccc
    19021 atataaagtg atagtcattc acagcactga taatacgtgt agcgataaaa cgagcgcttt
    19081 caacattgca gtctggcagc agcagaccaa attcatcacc gccgagccgc gccagcacgt
    19141 cactggagcg cagcatactc agcatcaacg acgccagttc gcgcagcaaa gcgtcgcccg
    19201 ccgcatgccc ggcgctgtca ttcaccgctt taaagcgatc aagatcgata aacaccaggg
    19261 catgtcgctg atgcgtactg tttaccgttt gcagcaggat acgcagttgt ttctcaaaac
    19321 tggcgcggtt ggcgagatgc gtcagtgcat catgggaggc gctgtagctc agctggcgca
    19381 gcattttgcg tgattcggtg acgtcctgaa tcaccagaac cgaaccaata ttgctgccgt
    19441 ccagagtact taacggcgta atactgtaat gcacgtcgta actgccgccg ctccgacagt
    19501 gcaacaccac atcttgttcg attgcggaac gtgaggtgtc ggcactgtaa atgttctcca
    19561 ttaatggtcc gttgtcgcca aaagtaatat gcaacaccgt caggagcgga acacctaacg
    19621 cttcttcctg cgtccagccg ctcatcttct ccgccactgg attcataaag gtaattttca
    19681 tcgccatatc aatacagacc acggcttcac cgatggaatc aagcgtaatg tgcaggcgct
    19741 ctttttcctg aaacaatgcc tcgttaagct gtttcacttc ggtcatatcc atattgatgc
    19801 cgaggagacg ttcgacttcg ccttctttat tcagtacccg gttggcgagg gcgcggatat
    19861 ggcgaatacc gtcttttacg gtaatgcgaa attccagttt aaagggcgag cgtgattgca
    19921 acgaatcacg aatcactttt tcggcatgct ggcgatcttc cggcagcacg cactcgtacc
    19981 acacctgcca gttcggtttg atatgtggag gaatttcata cagctcgaac atccgcttat
    20041 cccagctaaa aacattcggc ttcaactccc tctcccagat gccaatcccg cccgcttcgt
    20101 tagccagcgt gatgcgctcc atcagttgct gattgacctg ttcggtgcgt tttagctcgt
    20161 taatgtcttc aatctgagcg ataaaataga gcggcgtgcc atccgtgtgg cgcaccagtg
    20221 agacggcaag caacgcccag acaacatcgc cattgcggtt gtagtagcgt ttttccattg
    20281 aataggtgtt tatttcaccg cttatcagct tttcaacctg ttggagatct ttattgagat
    20341 cctccggcca ggtcagttgc tgaaaggtga gtccgcgcag ctcttcctga ctgtacccga
    20401 gaaactggca gagcgctttg ttggtttgca gccattgtcc ctcggtgccc actaacgcca
    20461 taccgatagc ggaatattcc atcgcgttcc gaaagcgggt ttcgctttcg gaaatgtgtt
    20521 tgcgttccgc acgaaaggca tacatcacca tggtcatgat gttggcgggc agcaggatca
    20581 gcaaaaacgg tagccacggc atatggctca tcaggtacgt acgcggcgta gcaagcaggg
    20641 agggatccgc ggccatcatc agcgacacca tcatcaccgt ggtaaggaag atcaaaaagg
    20701 cttccattcg tggcaggcgc acggcgctcc acatcaacag cacaataatg aaagtaaaag
    20761 gccacggcag atacagcatc gaaagccagc ttaacgtcag tgtgatggct aacgtgagca
    20821 gcgactcaaa aagcaaccgt gggttgcgat ggcgcagcag atagtgtggt ttaaataaca
    20881 atcccagcgg caccagtgcc agagcgccga tggattctga cagtacccat atcaaaaatg
    20941 ccctgagagg atcgtctccg ggcgtcagca ggacaaccag aacacccccc aacagaggcg
    21001 gaacaatggc gctgccgagt gccagacgca gccagtcagc cagattttgc aaggggttat
    21061 accacggcag caatttacgt agcagcactg ccccgaccac ggcttcaaca atattgatgg
    21121 tcgtccaggt catgttcagc gagctggtgg aaaaaagcag gatggatgcg gcgatatttc
    21181 ccagcgaaca gctcagcgca attcccggcc acatgcgccc ggcatggcga taaaacgcca
    21241 ccatcatgat ggacgtcggg aaccacagtg gggcgagttg ggtgccaaac tgcgaaagct
    21301 caagcgagaa aagggtaaag ataaacgaga ctaaacctaa actgaccagg tgaagcagcg
    21361 ggtggggcag ggcaattaat acatgctgtg attgtttgct cattaccgct ttatccgcaa
    21421 gaccaggtcg ccagtattcg tttctgtgtt tatgctagta caaaagtttt acattttata
    21481 tgcgaattgc tcataaagtg acgtaaaggc ggataatttg cgcaactgcg tataacattt
    21541 tttaccttac ataaaactga tcaacgtaat ttgccaccgg gaattctttg tcaacaatta
    21601 actctgagcg cgcggcaacg ctattcgact ggtatcagac ggatgaaatc cctataattg
    21661 ccgcgtttgg cgcttcgtcg cccccttcct aacatccagg ttaatcaggt cgctaaatat
    21721 atgactgatc agtctcatca gtgcgtcatt atcggtatcg ctggcgcatc ggcttccggc
    21781 aagagtctta ttgccagtac cctttatcgt gaattacgtg agcaagtcgg tgatgaacac
    21841 atcggcgtaa ttcccgaaga ctgctattac aaagatcaaa gccatctgtc gatggaagaa
    21901 cgcgttaaga ccaactacga ccatcccagc gcgatggatc acagcctgct gcttgagcat
    21961 ttacaagcgt tgaaacgcgg ctcggcaatt gacctgccgg tttacagcta tgttgaacat
    22021 acgcgtatga aagaaacggt gacggttgag ccgaagaagg tcatcattct cgaaggcatt
    22081 ttgttgctga cggatgcgcg tttgcgtgac gaacttaact tctccatttt cgttgatacc
    22141 ccgctggata tctgcctgat gcgccgcatc aagcgtgacg ttaacgagcg tgggcgttca
    22201 atggattccg tgatggcgca atatcaaaaa accgtgcgcc cgatgttcct gcaattcatt
    22261 gagccttcta aacaatatgc ggacattatc gtgccgcgcg gcgggaaaaa ccgcatcgcg
    22321 atcgatatat tgaaagcgaa aataagtcag ttctttgaat aagcttgata aattgtgtac
    22381 cgttcagtga taacctggta tgcccttgac gtaattggcg ttaagggagt gatgcgcgaa
    22441 aggagaaaat gccatgcgtc tgtgtgaccg agatattgaa gcctggcttg atgaaggccg
    22501 tttgtcgatc aacccacgtc cgccagtgga gcgtattaac ggcgcgacgg tggatgtacg
    22561 cctgggcaat aaatttcgta ccttccgtgg tcacacggca gcgtttatcg atctgagcgg
    22621 tcccaaagat gaagtgagcg ccgcgcttga ccgcgtgatg agcgatgaga tcgttctcga
    22681 cgagggcgag gcgttctatc ttcacccagg agagctggcg ctggcggtga cgctggagtc
    22741 ggtgacgctg ccagccgatc tggtgggctg gctggacggg cgttcctctt tggcgcgtct
    22801 ggggctgatg gtgcacgtca ccgcgcaccg tatcgatccg ggctggtctg gttgcattgt
    22861 gctggagttc tacaactccg gtaagctgcc gctggcgctg cgtccgggca tgttaattgg
    22921 tgcgctgagc tttgagccgc tttccggccc ggcggcgcga ccttacaacc gccgtgaaga
    22981 tgcgaaatat cgcaaccagc agggcgcggt agccagccga atcgataaag actaattcag
    23041 gcccattgag gataccatga gacgatttct gacgacgctg atgatactcc tggtcgtgct
    23101 ggtggccggg ttatctgtgt tagtgttgct ggtgaatccg aatgatttcc gcgactatat
    23161 ggtcaagcaa gttgctgcac gtagcggtta tcaattgcag ctcgacgggc cactgcgttg
    23221 gcacgtctgg ccgcagctta gtatcctctc cgggcgaatg tctctcaccg cccagggcgc
    23281 aagccagcca ctggttcgcg ccgacaacat gcgtctggac gtggcgcttt taccactact
    23341 gagtcatcaa ctgagcgtta agcaggtgat gctaaaaggg gcagtgatcc aactgacgcc
    23401 gcagacggaa gcggtgcgca gtgaagacgc tccggttgca ccgcgcgaca ataccttgcc
    23461 ggatctgtca gacgatcgcg gatggtcgtt tgatatatcc agtcttaagg tggcggacag
    23521 cgtgctggtg ttccagcatg aagatgacga gcaggtgaca atccgcaata tccgcctgca
    23581 aatggaacaa gatccccaac atcgtggctc atttgagttc tccgggcggg ttaatcgcga
    23641 tcagcgcgat ctcacaatat cccttaacgg tacggtggat gcttctgatt atccgcatga
    23701 tttaacggcg gctattgaac aaattaactg gcagttgcag ggtgccgatt taccaaaaca
    23761 aggtattcag gggcagggga gtttccaggc ccagtggcag gagtcacata aacgcctttc
    23821 atttaaccaa atcagcttga ccgccaatga cagtacgctg agcgggcaag cacaggtcac
    23881 gctgacagag aaaccggaat ggcagctgag actgcaattc ccgcaactga atcttgacaa
    23941 cctcatcccg cttaatgaaa ccgcgaatgg tgaaaacggt gccgcgcagc aggggcagag
    24001 ccaatcaacg ttgccgcgcc cggtcatttc ttcgcgtatt gatgaaccgg cctatcaggg
    24061 actgcaaggc tttacggctg atattttgtt gcaggccagt aacgtgcgct ggcgcggaat
    24121 gaattttaca gatgttgcca cgcaaatgac caacaagtcg ggtttgctgg aaattactca
    24181 actgcagggc aaacttaacg gtggacaggt ttcactgccg ggcacgctgg acgcgacatc
    24241 aataaatccg cggataaact tccagccacg gctggaaaac gttgagattg gtaccattct
    24301 gaaggcgttt aactatccga tttcgttgac cggaaaaatg tcactggctg gtgatttctc
    24361 cggtgctgac atagatgccg acgcattccg ccacaactgg caaggacagg cacatgtcga
    24421 aatgaccgac acgcgcatgg aagggatgaa cttccagcag atgattcagc aagcggtaga
    24481 gcgtaatggc ggtgatgtga aggccgctga aaactttgat aacgtgacgc ggcttgaccg
    24541 ctttaccacc gatttgacgt tgaaggatgg cgtcgtgacg ttaaacgaca tgcaaggtca
    24601 atcgcctgtg ctggcgctga caggggaagg catgttgaat ctggcagatc aaacctgcga
    24661 tacccagttt gatattcggg tcgtgggtgg ctggaacggg gaaagtaaac tgattgattt
    24721 cctgaaagaa acgccagtac cgctgcgggt ttatggcaac tggcagcaac tcaattacag
    24781 cctgcaagtg gatcagttac tgcgcaaaca tctacaggac gaagcgaaac gtcgcctgaa
    24841 tgactgggcc gagcggaata aagattcccg caatggcaag gatgtgaaga agttgctgga
    24901 gaagatgtag gttaccaggt gaccagtcct gtaggcctga taagacgcgt aaagcgtcgc
    24961 atcaggcgtt cgtggcggca tcattatatt ctgaatatgt aagatcgaag gctgttccgt
    25021 tcacttgatg ttcctcgcta ctatggaacg ctcaagccgg tgaacgtgcc agggtggctc
    25081 aatcgccacc accctggcaa cccgggctcc cggcggtaaa tcgccgctgg cgcggttccc
    25141 tcatcttatt cctttcggct attgggtaaa atgaccgata ccatcacacc tcgtactcca
    25201 actcgccatc cttatgcagc ggaataatct gcaccttctg cacgcgatgg ctttccacct
    25261 gcaacgtttt aagcaaataa tcccccacct gaacttcttc gccaggtttt ggaatacgct
    25321 gtaaatattc catcaacagc ccggcaatgg tgtgatattc acgtttttca tccagcggca
    25381 gcggcacata ttgcaccaga tcttccagcg gcatatgacc attcgccgtc caggaaccgt
    25441 ccgcattctt ctgaatatca tggcgggcgt cgatctcttc cacttcgttc ggtaagttac
    25501 cgacaatggt ttcagtgacg tcacttaatg tcacaatccc ttccaccgag ccaaactcat
    25561 ccaccacaaa agcaaagtgc gtgcgggcat tacggaactg ctccagggca ggtaacaacg
    25621 gcaaggtttc cgggaacacc agcggctggc gaatcaacac ccgcaggttg agcggttcgc
    25681 cgcgcagaga ctgttgtagc aggtcgataa cgtgaacaac accgagcaaa tcttctgcgt
    25741 catcaccgtc ggtaaccacc agccgcgtat gctgatttcg ctccagcaac tggcggattt
    25801 cttcttccgg cgcgttgaga tcgatatgct caatatcgtg gcgcgacgtc ataatgctgc
    25861 tgacggtacg ctggttaaga ttaagtaccc gctcaatcat ccgccgttcc tgcggattaa
    25921 agatctgctg gttaccatga tccaccagca tggacgcggt ttcggcgtcc agttccgcat
    25981 cttctttttg cccgctaagc aggcgcatta ccgcttcggt agtccgctgg cgcagcgtct
    26041 ggttagcgga aagaaaacgt cgccggttaa agatagccaa ctgattgagc gcctcgatca
    26101 tcaccgagaa accaatggca gcgtacaggt agcctttcgg aatgacgaag ccgaaacctt
    26161 ctgccaccag gctaaagcca atcatcaaca ggaagcttaa gcagagaata acgatcgtcg
    26221 ggtgactgtt aacaaattgc gttaacggct tgctggccat caacatcagg ctgatagcga
    26281 taaccacggc ggccatcatg accagtaaat ggtcgaccat cccgacggcg gtaatcactg
    26341 agtcgagtga gaaaatggcg tccagtacca caatttgcgt caccacgccc cagaacttcg
    26401 cacctttgcg ttgtgtggga ttattgctgt ctttcccttc cagccgttcg ttcagctcca
    26461 tcgtggcttt gaacaacagg aagaaaccac caaacagcat gattaagtcg cgggcgctaa
    26521 aggtaaacga gcggaagctg aacagcggtt gagtcagggt gaccagccag gagattgacg
    26581 ccagcagtaa caggcgcatt aacatcgcca gcagcagccc ggtaacccgt gcgcggtcgc
    26641 gctgcctcgg cggtagtttt tcggcgagga tggcaataaa gaccaggtta tcaatgccga
    26701 ggaccagttc gatcacaatc agcgtgatta acccggccca gatagacgga tcggcaatcc
    26761 attccatagt aagtacaaaa cctattcgtt gtatgacaaa tgtcacaatt cgatcatgga
    26821 taaatcgggg caaaaatgca atgttgcagc actatttatc tca
//
LOCUS       DAZSBF010000036        26850 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZSBF010000036 DAZSBF010000000
VERSION     DAZSBF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.036"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHXWD_001159"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHXWD_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR7620131.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHXWD_001160"
     CDS             567..650
                     /locus_tag="ACHXWD_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620132.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHXWD_001161"
     CDS             750..1571
                     /locus_tag="ACHXWD_001161"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR7620133.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHXWD_001162"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHXWD_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR7620134.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHXWD_001163"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHXWD_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR7620135.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHXWD_001164"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHXWD_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR7620136.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHXWD_001165"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHXWD_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR7620137.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHXWD_001166"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHXWD_001166"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR7620138.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHXWD_001167"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHXWD_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR7620139.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHXWD_001168"
     CDS             8347..9729
                     /locus_tag="ACHXWD_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7620140.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHXWD_001169"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHXWD_001169"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR7620141.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHXWD_001170"
     CDS             complement(10485..10820)
                     /locus_tag="ACHXWD_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR7620142.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHXWD_001171"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHXWD_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR7620143.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHXWD_001172"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHXWD_001172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR7620144.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHXWD_001173"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHXWD_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR7620145.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHXWD_001174"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHXWD_001174"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR7620146.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHXWD_001175"
     CDS             complement(15521..17167)
                     /gene="fumB"
                     /locus_tag="ACHXWD_001175"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR7620147.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17366..18541
                     /gene="manA"
                     /locus_tag="ACHXWD_001176"
     CDS             17366..18541
                     /gene="manA"
                     /locus_tag="ACHXWD_001176"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR7620148.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18642..20150
                     /locus_tag="ACHXWD_001177"
     CDS             18642..20150
                     /locus_tag="ACHXWD_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR7620149.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHXWD_001178"
     CDS             complement(20331..21641)
                     /gene="uidC"
                     /locus_tag="ACHXWD_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR7620150.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHXWD_001179"
     CDS             complement(21680..23053)
                     /gene="uidB"
                     /locus_tag="ACHXWD_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR7620151.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGFCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHXWD_001180"
     CDS             complement(23050..24861)
                     /gene="uidA"
                     /locus_tag="ACHXWD_001180"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR7620152.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHXWD_001181"
     CDS             complement(25250..25840)
                     /gene="uidR"
                     /locus_tag="ACHXWD_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR7620153.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHXWD_001182"
     CDS             complement(26061..26828)
                     /gene="hdhA"
                     /locus_tag="ACHXWD_001182"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR7620154.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6738 a         6952 c         6458 g         6702 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgat ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tagctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggccgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatga aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc ggctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg gcggctctgt ttatttcaca cagcgagtgc
    15541 actgtgtgag ttgtatctgc tggaagaagt catttccttt atcatccaca aggataaacg
    15601 ccgggaaatc ttccacttca attttccaga tggcttccat tcccagttcc ggatattcaa
    15661 cacattccag gctcttaata cttccctgcg ccaatacagc ggccggacca ccgatactgc
    15721 caaggtagaa gccgccgtgt tttttacagg catccgtcac ctgctggctg cggttgcctt
    15781 tcgccagcat gatcatactt ccgccctgcg cttgcagttg atcgacataa gaatccatcc
    15841 gtccggcggt cgttgggcca agagaaccgg aggcataacc ttccggcgtt ttggccggac
    15901 ccgcgtagta aatcggatga tctttaatgt actgcggcag cccttcaccg ttatccatcc
    15961 gctctttcag tttggcgtga gcaatatcac gaccgacgat aatcgtgccg ttaagcgata
    16021 agcgtgtaga aacgggatac tgcgacaact gtgcgaggat ctctttcatc ggacggttaa
    16081 ggtcaacgcg caccgcttcg ccttctcccg ctttacgcag ctcttcaggg atatatttac
    16141 ctggattatg ttccagtttt tcgatccaga tcccctgacg gttgatcttc gctttgatat
    16201 tacggtcagc agagcaggaa acacccatac caaccggaca ggatgcgccg tgacgtggca
    16261 ggcgaatcac gcgtatgtcg tgagcgaagt atttaccacc aaactgcgca cccagaccga
    16321 gattttgcgc ttcgatcagc aattcttttt ccagttccac atcgcggaat gcctgaccgt
    16381 gctcattccc ttccgttggc agttcgtcat agtatttagc ggaagccagt ttcactgttt
    16441 taaggttagt ttctgcagaa gttccaccaa ttacgaacgc aatatgatac ggaggacagg
    16501 ccgccgtacc cagcgtgcgc atcttctcaa ccaggtaatt tttcagtttc cccggcgtta
    16561 gtaacgcttt ggtttcctga tagagatacg tcttgtttgc cgaaccacca cctttggcga
    16621 tacagaggaa tttgtactcg tcgccatcaa cggcataaag atcgatctgc gctggcagat
    16681 tggtgccggt attcacctct ttatacatat ccagcggcgc gttttgcgag tagcgcaaat
    16741 tatcttcgat ataagtgtta tagacaccgc gcgccagcgc cgcttcatca cagccaccgg
    16801 tccatacacg ctgccctttt ttaccaacaa taatcgcagt gccggtatcc tgacaggttg
    16861 gcagaacacc tttcgccgcg atgtcggagt tgcgcaggaa ttgcagcgcc acatatttat
    16921 cattttcgct ggcttccggg tcacgcagaa tgtcggccac ctgttgttgg tgagccggac
    16981 gcagcataaa tgacgcatca tgaaacgcct gacgcgccaa cagagttaac gcttcgggtg
    17041 cgactttcaa aatctcctgc ccttcaaatt cagatacgct aacgtgttcg ctggttagca
    17101 ggtaatactc agtatcatct tttttgagtg gaaaaggagc ctgataatga aagggtttgt
    17161 ttgacattgt tctctcactt actgcctggt ttggttatgc tctgggcggg tgttccgttg
    17221 ccctgttaaa agcgagtaac aatatcctac acactttttt aacaaaaact gagactagta
    17281 cgactttttg cggctccagg ttacttcccg taggattctt gctttaatag tgggattaat
    17341 ttccacatta aaacagggat tgatcatgca aaaactcatt aactcagtgc aaaactatgc
    17401 ctggggcagc aaaacggcgt tgactgaact ttatggtatg gaaaatccgt ccagccagcc
    17461 gatggccgag ctgtggatgg gcgcacatcc gaaaagcagt tcacgagtgc agaatgccgc
    17521 cggagatatc gtttcactgc gtgatgtgat tgagagtgat aaatcgactc tgctcggaga
    17581 ggccgttgcc aaacgctttg gcgaactgcc tttcctgttc aaagtattat gcgcagcaca
    17641 gccgctctcc attcaggttc atccaaacaa acgcaattct gaaatcggtt ttgccaaaga
    17701 aaatgccgca ggtatcccga tggatgccgc cgagcgtaac tataaagatc ctaaccacaa
    17761 gccggagctg gtttttgcgc tgacgccttt ccttgcgatg aacgcgtttc gtgagttttc
    17821 cgagattgtc tccctactcc agccagtcgc aggtgcacat ccggcaattg ctcacttttt
    17881 acaacagcct aatgccgaac gtttaagcga actgttcgcc agcctgttga atatgcaggg
    17941 tgaagaaaaa tcccgcgcgc tggcgatttt aaaatcggcg ctcgacagtc agcagggtga
    18001 accgtggcaa acgattcgtt taatttctga attttacccg gaagacagtg gcctgttctc
    18061 cccgctgtta ctgaatgtgg tgaaattgaa tcctggcgaa gcgatgttcc tgttcgctga
    18121 aacaccgcac gcttacctgc aaggcgtggc gctggaagtg atggcgaact ccgataacgt
    18181 gctgcgtgcg ggtctgacgc ctaagtacat tgatattccg gaactggttg ccaatgtgaa
    18241 attcgaagcc aaaccggcta accagttgtt gacccagccg gtgaaacaag gtgcagaact
    18301 ggacttcccg attccagtgg atgattttgc cttctcgctg catgacctta gtgataaaga
    18361 aaccaccatt agccagcaga gtgccgccat tttgttctgc gtcgaaggcg atgcaacgtt
    18421 gtggaaaggt tctcagcagt tacagcttaa accgggtgaa tcagcgttta ttgccgccaa
    18481 cgaatcaccg gtgactgtca aaggccacgg ccgtttagcg cgtgtttaca acaagctgta
    18541 agagcttact gaaaaaatta acatctcttg ctaagcttat taaaggctta taacaccttc
    18601 aggcggccag tccgcctgat ttcattttat ggataatcat tatgaataaa tcgctggtag
    18661 cggtaggcgt cattgttgcg ctaggcgtag tctggacagg cggcgcatgg tatacaggca
    18721 agaagattga aacccatctc gaagacatgg tcgcgcaggc gaacgcgcaa ctcaaactga
    18781 cagctcctga atccaacctg gaagtgagtt atcaaaacta tcatcgcggc gtattcagca
    18841 gccagttgca actgttggtg aaacccattg ccgggaaaga aaatccgtgg attaaaagcg
    18901 gtcagagcgt catcttcaac gaatcggttg atcatggtcc cttcccgctt gcccagctta
    18961 aaaaactgaa cctgatcccg tcgatggcat caattcaaac cacgctggtt aataacgaag
    19021 taagcaaacc actgtttgat atggcaaaag gtgaaacgcc ttttgagatt aactcgcgca
    19081 ttggttacag cggtgattcc agttccgata tttcgctcaa gccactgaat tacgagcaaa
    19141 aggatgaaaa agtcgccttt agcggcggcg agttccagtt aaatgctgac agagacggca
    19201 aagccatctc cctttccggg gaggcgcaaa gtggtcggat agacgcagtt aacgaataca
    19261 accagaaagt gcagttgacc tttaataatc tgaaaaccga cggttccagc acgctggcaa
    19321 gttttggtga gcgcgtagga aaccaaaaac tgtcactgga aaaaatgacc atttcagtgg
    19381 aaggcaaaga actggcactg ctggaaggca tggagatcag cggtaaatcg gatctggtca
    19441 atgacggtaa aacgatcaat agccaactgg attactcgct aaacagcctg aaggtacaga
    19501 atcaggatct gggcagcggc aagctgactt taaaagtcgg ccagattgat ggtgaagcct
    19561 ggcatcagtt tagccagcaa tataacgcgc aaactcaggc gctgctggca cagccagaaa
    19621 ttgccaacaa tcccgaactt tatcaggaga aagtgacgga agccttcttt agcgccctgc
    19681 cgctgatgtt gaaaggcgat ccggtgatta ctatcgcgcc gctaagctgg aaaaacagtc
    19741 agggtgaaag tgcgctgaat ctgtcgctgt tcctgaaaga tccggcaacg actaaagaag
    19801 cgccgcaaac gctggcgcag gaagtagatc gttcggttaa atctctggat gcgaaactga
    19861 ccattccggt ggatatggca actgagttta tgactcaggt agcgaagctg gaaggttatc
    19921 aggaagatca agcgaaaaaa ctggcgaaac agcaagttga aggtgcatca gcaatggggc
    19981 agatgttccg tctgaccacc ttgcaggaca ataccatcac caccagcctg caatatacta
    20041 acggtcagat aacgttaaac gggcagaaaa tgccactgga agatttcgtt ggtatgtttg
    20101 cgatgccagc tcttaacgtt ccggctgtac cggcaattcc gcagcagtaa ttcacggcca
    20161 aaacgccgga gaattttcac ttcggcgttt tcatctgaaa acaaaccaga gattgcctga
    20221 tgcgctacgc ttatcaggcc taccttttcc tgcaacactt tgaatttatg agtttttgta
    20281 ggctggataa ggcgtttaca ccgcatccgg cataaaaaac gcgcactttg tcaacaatct
    20341 gaaacgccgg aggtttttct ctccggcgtt atttctgact tcagcataaa gtcatactga
    20401 atactcattt tcacatccca gtaattcagg cgatcgccca gcgtactgcc atcgcgcttt
    20461 tcccgccctt tatcggcagc aagaaagaaa ttcatccctt gtaacggccc ttcgctgacc
    20521 cgatagttca taccacctaa gatacccgcg ccttcaatcc gcacatctcg tttactggat
    20581 tcatagccgc gagtcacaat ctgtgcaaat gtcagtgtaa attgcggcgt taaacgatag
    20641 ttgacgccca attgccagga ctgcattcct tcacggttac gatcaagact taatgaaaag
    20701 ggatagccaa tgtcggtatc caccacggca ccattcattg catggcgcaa ataaccacca
    20761 ctgccaaata cgcttcccca cggcgcattc caggtcaatt gtccactaac caacgccgtt
    20821 tcattgggct ggctggtatt acgacttaaa ccttcgagct gcgcgtaaaa gccgagcaac
    20881 tcccctttca acgtataatt cgggtcgagc ggtatgcttt tctcgagaat actttgtatc
    20941 tgattggaaa catctttctg taccgatgcc cccagagaat agcgactggt atcatcatgc
    21001 caactggcgg ctaaaaaaga acgtgcgcga tgtttatcgt attttcccgt ctggctgtta
    21061 tacacccctt catcacgaat accggttgtt gaatcagtcc ataaaaatac tcgtgaacgc
    21121 cacattcccc acagatcaat atttggtgtt aaagcccaat aacctaatgc tccttcagaa
    21181 actgtcggta atgtacgttg cgaactgcca gccattaacg gaatacgata aggtaagcta
    21241 ccataactat aatccgtccc gaagcgacct gatttaagca ggctattttc actgggtttt
    21301 atttttactg cccccagggc aaagccaaaa cttttatcac catcaagata ccaccgggta
    21361 ctcatatcag cacgagcacc taatttataa acataatatg ccccgccttc aacgccaata
    21421 atattggaat aataaccact attgaaatcg agcaatcctc cctgtaccca tgcgtaaata
    21481 tctcttcccg cgccagcact gggcttatcc gccctgcgca attcgttttt cagtctgatg
    21541 cgtagtcccg cctcatcgtc agccagttgc gccgcataag cagagataag gccagaggca
    21601 gccgtcaggc aaataacggc catggccact atttttctca tagttgcacc tttgatgttc
    21661 tgattatttt tattgattat taattagtga tatcgctgat taattgctgc tgcacttttt
    21721 tacgattatc aatttcaacc acgatttctt tgaatttttt atccgtaagc ggataaaacc
    21781 agataataac gaatgccagt agcataaatc cgcaaggtac taaggcaatt gatgtgcgga
    21841 tgcccataat aacttccggc gtttgcacct gattggcaat atatccgctt aaccccaaaa
    21901 taaaggcagg aattgaacct ccgattgcct gaccgcattt acgggtaaat gagaatagtg
    21961 aataggtgag cccttcaatt cgcacgccgg tcaggtattc accgtattct acggtatcag
    22021 cttccagtgc ccacatcact gtcatagtaa cgccctgacc aattgaagcg atggccaacg
    22081 caaccaacgc caccggcagt gaccagacgg aaacccagaa gaatagcaga taaccgcagg
    22141 ttcccagcaa agcgccaatc aggaaggtat tctttttacc gatcctcgcg accatccccg
    22201 gcacaagcgg tgccgatgcc acagtaccaa ccaggttttg caccagtacc agcacagtga
    22261 acagcccggt atcatttaac acatagcgca cgtagaacaa cgacgaggcg ctgaccgcaa
    22321 aggtcgaaat cagcacacac agcgcaccga tgcacaacat aaacagcggg cgattccgtt
    22381 tcagggtttg caaactgatc ttcaatgacg gctgcgcaac gatacgtacc acattctcac
    22441 gcgtcgattt gaagcagatg aagtaaagca ccattccggc aatcgccagc acaatcgtcc
    22501 agaaatggta taccgacacc atctcttccg ggctggagtt cttaatgctc ggccctatca
    22561 gaaatgccag gcagacaaag gtcaatgaag cggcaatccc acgagccgcg cccagacggg
    22621 cgcgggattg tggttgttgg gtcatcgcgg tagcaagtga accataagga atattcacca
    22681 ggctgtagca aaacccgagg cccatgtagg tcaaatatgc atacactact ttgcggctat
    22741 ggctccagtc ggtcggtacc cagaatacca gcacgctgaa gatcattaac ggcgcagtac
    22801 cgaagagtaa aaacgggcgg aattttcccc agcgggtatt cacactgtcc accactcgtc
    22861 cggcaaagac gtcggcgaag gcatcgaata cccgcaccag taacagcatg gtgcccgccg
    22921 cagcggcacc gacgccagcg acgtcggtgt agtaactcaa caggaagagc acccccattg
    22981 cgaaggcgaa gttattggcg acgtcaccga ggctgtagcc gacgatggtg cgccaggaga
    23041 gttgttgatt cattgtttgc ctccctgctg cggtttttca ccgaagttca tgccagtcca
    23101 gcgtttttgc agcagaaaag ccgccgactt cggtttgcgg tcgcgggtga agatcccctt
    23161 cttgttaccg ccaacgcgca atatgccttg cgaggtcgca aaatcggcga aattccatac
    23221 ctgttcaccg acgacggcgc tgacgcgatc aaagacgcgg tgatacatat ccagccatgc
    23281 acactgatac tcttcactcc acatgtcggt gtacattgag tgcagcccgg ctaacgtatc
    23341 cacgccgtat tcggtgatga taatcggctg atgcagtttc tcctgccagg ccagaagttc
    23401 tttttccagt accttctctg ccgtttccaa atcgccgctt tggacatacc atccgtaata
    23461 acggttcagg cacagcacat caaagagatc gctgatggta tcggtgtgag cgtcgcagaa
    23521 cattacattg acgcaggtga tcggacgcgt cgggtcgagt ttacgcgttg cttccgccag
    23581 tggcgcgaaa tattcccgtg caccttgcgg acgggtatcc ggttcgttgg caatactcca
    23641 catcaccacg cttgggtggt ttttgtcacg cgctatcagc tctttaatcg cctgtaagtg
    23701 cgcttgctga gtttccccgt tgactgcctc ttcgctgtac agttctttcg gcttgttgcc
    23761 cgcttcgaaa ccaatgccta aagagaggtt aaagccgaca gcagcagttt catcaatcac
    23821 cacgatgcca tgttcatctg cccagtcgag catctcttca gcgtaagggt aatgcgaggt
    23881 acggtaggag ttggccccaa tccagtccat taatgcgtgg tcgtgcacca tcagcacgtt
    23941 atcgaatcct ttgccacgca agtccgcatc ttcatgacga ccaaagccag taaagtagaa
    24001 cggtttgtgg ttaatcagga actgttcgcc cttcactgcc actgaccgga tgccgacgcg
    24061 aagcgggtag atatcacact ctgtctggct tttggctgtg acgcacagtt catagagata
    24121 accttcaccc ggttgccaga ggtgcggatt caccacttgc aaagtcccgc tagtgccttg
    24181 tccagttgca accacctgtt gatccgcatc acgcagttca acgctgacat caccattggc
    24241 caccacctgc cagtcaacag acgcgtggtt acagtcttgc gcgacatgcg tcaccacggt
    24301 gatatcgtcc acccaggtgt tcggcgtggt gtagagcatt acgctgcgat ggatcccggc
    24361 atagttaaag aaatcatgga agtaagactg ctttttcttg ccgttttcgt cggtaatcac
    24421 cattcccggc gggatagtct gccagttcag ttcgttgttc acacaaacgg tgatacgtac
    24481 acttttcccg gcaataacat acggcgtgac atcggcttca aatggcgtat agccgccctg
    24541 atgctccatc acttcctgat tattgaccca cactttgccg taatgagtga ccgcatcgaa
    24601 acgcagcacg atacgctggc ctgcccaacc tttcggtata aagacttcgc gctgatacca
    24661 gacgttgccc gcataattac gaatatctgc atcggcgaac tgatcgttaa aactgcctgg
    24721 cacagcaatt gcccggcttt cttgtaacgc gctttcccac caacgctgat caattccaca
    24781 gttttcgcga tccagactga atgcccacag gccgtcgagt tttttgattt cacgggttgg
    24841 ggtttctaca ggacgtaaca taagggactc ctcattaaga taaaaatact ggtcaacctt
    24901 taatctgatt agttaaccaa ttctgtgttg cacacagtta tctgtgagaa acccatggaa
    24961 gagaaatgga aataaaaaga actgacaccg cgatcaaaaa accaaaaact gcaataccca
    25021 cagcgtgata ttgatcgcat taatctttaa aacggcgttg acagaagcca taaaataagc
    25081 aaccgaacgt tcgttcgagc atattgggaa cgataaactc tcatgacata tggctacggt
    25141 gaatattttt gggggggaat tctccgatcc cggctagatt gtattttgta cagggaaatt
    25201 aaagtatgaa acgcgggcac agttatcacc agcccgcgaa atgagaggat caggatgcgg
    25261 ttaagatacc gccaatcatc gcgcgtaatc cctgagcaaa cgacgcttca cgcgccagat
    25321 cttccgcttc gatatcggcc agtacaccgt aggtcagatc cagcagtaaa cgtgaagtca
    25381 ttgacgtgtt gatgtctggg cttatctcgc ctttttgctg ggcatcacgc atccgctggg
    25441 caacaaattc cgtgatcgtc atatgcttat ttttaagcat ggcggcgacc tgtgggttac
    25501 gcatcccttc cgccataatt tcgaccacca gcgcccgttg cccaaaggct tcatgggtga
    25561 gagagacaat agactcgacc atatagtcaa caaaatgaat ccctttaatc ggttcccgga
    25621 aacgagccag cgccctctcc tggtcctgta agataatcgc ctgaatcaag gcttctttgg
    25681 agatgaaatg gtgatagagc gtcccgggac taatggcgca agatttacag atggctttca
    25741 tcgaggcact gtgaaatcca ttttctgaaa aaatctctct ggcagcattg aggatccggg
    25801 tccgtgtcgg ttgtgcttca gtctgcatgt tatccatcat taaaccatta tagttagcgt
    25861 gaataactag tttaccgcgt tgtcaggtga gcgcctatca ttgctgttga atagtgcgaa
    25921 ggcacactct attcgtatac ttttcactct gaaattggtt aaccaatagc cgtgatttgg
    25981 ctgccagtta tttagcagag acgttgatga gtttatcccg gtaaacaaca cgcacggttg
    26041 tttaccggtc cgttagtgta ttaattgagc tcctgtaccc caccaccgga gacggtgaga
    26101 atttgtccgc ttacccagct cgcagcaggc gagcaaagga acagcgctgc gttagcaata
    26161 tcttgcggtt ggcccagacg tctgatcggc gtgtgctgta acattttttg ttcaatttct
    26221 ggtgtaataa cggatttcag ggcatcggtt aatattgccc ccggcgcaat gccatttacc
    26281 cgaatatttt tttcacccag gtcaaacgcc atatttctga ccagatgact ggctgcagct
    26341 ttagatgatg cataggaagt catgtttata tttttatttt ctgccgccat agaagtgatg
    26401 gtcagaataa cgccaccgcc atttttttcc atttctggcg caacaagttg tgacagatgg
    26461 aaaaaagaaa acacattcag ttcataagca cggcgaaaat ccgccattgg catatcaaac
    26521 ggtttaggtc caccgccacc ggcgttgtta accagaatat caactttacc cagcttactg
    26581 atagcaaagt ctgccagtgc agagagttcc tgttcggaag taatatcaca acggcaggca
    26641 aatgcctgac cacccagttg ttgaatttcg tctacaacat ggttagctgc gtcggcgtta
    26701 atatcactga ccaccacaga tgcgccagct gtcgcgaatg taatggcgat ttctttacca
    26761 atacctgcac ccgcacctgt gatgatggcg catggcgcat ggcgcatggc gcatggcgca
    26821 tggcgcatgg cgcatggcgc attttccgtc
//
LOCUS       DAZSBF010000037        26555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZSBF010000037 DAZSBF010000000
VERSION     DAZSBF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.037"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            48..371
                     /locus_tag="ACHXWD_001183"
     CDS             48..371
                     /locus_tag="ACHXWD_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR7620155.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKKIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(474..1724)
                     /gene="icd"
                     /locus_tag="ACHXWD_001184"
     CDS             complement(474..1724)
                     /gene="icd"
                     /locus_tag="ACHXWD_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR7620156.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1896..2549
                     /gene="rluE"
                     /locus_tag="ACHXWD_001185"
     CDS             1896..2549
                     /gene="rluE"
                     /locus_tag="ACHXWD_001185"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR7620157.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2559..3020
                     /gene="nudJ"
                     /locus_tag="ACHXWD_001186"
     CDS             2559..3020
                     /gene="nudJ"
                     /locus_tag="ACHXWD_001186"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR7620158.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3074..4180
                     /gene="mnmA"
                     /locus_tag="ACHXWD_001187"
                     /gene_synonym="trmU"
     CDS             3074..4180
                     /gene="mnmA"
                     /locus_tag="ACHXWD_001187"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR7620159.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4216..4857
                     /gene="hflD"
                     /locus_tag="ACHXWD_001188"
     CDS             4216..4857
                     /gene="hflD"
                     /locus_tag="ACHXWD_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR7620160.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4861..6231
                     /gene="purB"
                     /locus_tag="ACHXWD_001189"
     CDS             4861..6231
                     /gene="purB"
                     /locus_tag="ACHXWD_001189"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR7620161.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6399..7070
                     /gene="phoP"
                     /locus_tag="ACHXWD_001190"
     CDS             6399..7070
                     /gene="phoP"
                     /locus_tag="ACHXWD_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR7620162.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7070..8530
                     /gene="phoQ"
                     /locus_tag="ACHXWD_001191"
     CDS             7070..8530
                     /gene="phoQ"
                     /locus_tag="ACHXWD_001191"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR7620163.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8606..9727
                     /gene="roxA"
                     /locus_tag="ACHXWD_001192"
                     /gene_synonym="ycfD"
     CDS             8606..9727
                     /gene="roxA"
                     /locus_tag="ACHXWD_001192"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR7620164.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9776..11002)
                     /gene="pepT"
                     /locus_tag="ACHXWD_001193"
     CDS             complement(9776..11002)
                     /gene="pepT"
                     /locus_tag="ACHXWD_001193"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR7620165.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            11252..12388
                     /gene="potA"
                     /locus_tag="ACHXWD_001194"
     CDS             11252..12388
                     /gene="potA"
                     /locus_tag="ACHXWD_001194"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR7620166.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12372..13229
                     /gene="potB"
                     /locus_tag="ACHXWD_001195"
     CDS             12372..13229
                     /gene="potB"
                     /locus_tag="ACHXWD_001195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR7620167.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            13226..14020
                     /gene="potC"
                     /locus_tag="ACHXWD_001196"
     CDS             13226..14020
                     /gene="potC"
                     /locus_tag="ACHXWD_001196"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR7620168.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            14017..15063
                     /gene="potD"
                     /locus_tag="ACHXWD_001197"
     CDS             14017..15063
                     /gene="potD"
                     /locus_tag="ACHXWD_001197"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR7620169.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(15219..16040)
                     /gene="cobB"
                     /locus_tag="ACHXWD_001198"
     CDS             complement(15219..16040)
                     /gene="cobB"
                     /locus_tag="ACHXWD_001198"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR7620170.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16056..16967)
                     /gene="nagK"
                     /locus_tag="ACHXWD_001199"
     CDS             complement(16056..16967)
                     /gene="nagK"
                     /locus_tag="ACHXWD_001199"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR7620171.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16996..18240)
                     /gene="lolE"
                     /locus_tag="ACHXWD_001200"
     CDS             complement(16996..18240)
                     /gene="lolE"
                     /locus_tag="ACHXWD_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR7620172.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(18240..18941)
                     /gene="lolD"
                     /locus_tag="ACHXWD_001201"
     CDS             complement(18240..18941)
                     /gene="lolD"
                     /locus_tag="ACHXWD_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR7620173.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18934..20133)
                     /gene="lolC"
                     /locus_tag="ACHXWD_001202"
     CDS             complement(18934..20133)
                     /gene="lolC"
                     /locus_tag="ACHXWD_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR7620174.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20395..21468
                     /locus_tag="ACHXWD_001203"
     CDS             20395..21468
                     /locus_tag="ACHXWD_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR7620175.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21596..25042
                     /gene="mfd"
                     /locus_tag="ACHXWD_001204"
     CDS             21596..25042
                     /gene="mfd"
                     /locus_tag="ACHXWD_001204"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR7620176.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            25186..26142
                     /gene="ldtC"
                     /locus_tag="ACHXWD_001205"
                     /pseudo
     CDS             25186..26142
                     /gene="ldtC"
                     /locus_tag="ACHXWD_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(26225..26482)
                     /gene="bhsA"
                     /locus_tag="ACHXWD_001206"
                     /gene_synonym="ycfR"
     CDS             complement(26225..26482)
                     /gene="bhsA"
                     /locus_tag="ACHXWD_001206"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR7620177.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6495 a         6882 c         6720 g         6458 t
ORIGIN      
        1 aacatccaaa tgaactatat taaataccgt gggataagac ataacaaatg aagtgggtag
       61 taattgacac ggtaattcaa cctacatgcg gcatatcttt ttcagccata tggggtgata
      121 tgaaaaagat catctggtat caatctacta tatttcttcc tcctggaagt atatttacac
      181 cggttaagct tggtattatc cttaaggaaa aagaatatcc tattactatt tataacattg
      241 caccattcaa caagaattta tggagtttac tcaaaagcag tcaagagtgt cctccaggag
      301 aaagcaaaat aacaaataaa tgtttacata atagttgcat tataaaaata tgcccatatg
      361 ggctcaagta atgggcttca tataaatatg ctcgatatgc tattaataat atccacataa
      421 ataaaacaac gggcgtgtta tacgcccgtt tcaatattta actcatggag agattacatg
      481 ttttcgatga tcgcttcacc aaactctgaa catttcagca gcttagcgcc ttccatcaga
      541 cgttcaaagt cataagttac ggttttcgcg ttgatagcac cttccatacc tttaacaatt
      601 aaatcagccg cttcagtcca ccccatatgg cgcagcatca tctcagcgga gaggatgata
      661 gagcctggat ttactttgtc ctgaccggca tatttcggcg cagtaccgtg ggtggcttca
      721 aacagggcgc attcgtcacc gatgtttgcg ccaggggcga taccgatacc gccaacctgc
      781 gctgccaggg cgtcagaaat gtagtcaccg ttcaggttca tacaggcgat aacatcatat
      841 tcagccggac gcagcaggat ctgttgcagg aatgcatcag caatcacgtc tttaatgacg
      901 atctctttgc cggtgttcgg gtttttaact ttcagccacg ggccgccgtc gatcagttca
      961 ccgccaaact cttcacgcgc cagctggtag ccccagtctt taaacgcgcc ttcggtgaac
     1021 ttcatgatgt tgcctttgtg caccagagtc acagagtcac gatcgttagc aattgcgtat
     1081 tcgatcgctg cacgaaccag acgtttggtg ccttcttccg aacacggctt aataccgata
     1141 ccacaatgtt ccgggaagcg aattttcttc acccccatct cttcacgcag gaatttaatc
     1201 actttctcgg cgtcggcaga gtctgctttc cattcgatac ccgcataaat gtcttccgag
     1261 ttttcacgga agataaccat atcggtcagt tcagggtgtt taaccgggct tggagtgccc
     1321 tgatagtaac gtaccggacg caggcagatg tagagatcca gttcctggcg cagggcaacg
     1381 ttcagagagc gaataccgcc accaacagga gtggtcagcg gacctttaat ggcaacgcga
     1441 tattcacgaa tcagatcaag ggtttcagca ggcagccaga catcctgacc ataaacctgt
     1501 gtggattttt caccggtgta aatttccatc caggagattt tacgctcgcc tttataggct
     1561 ttctcgactg cagcgtcgac cactttcagc atggctgggg ttacatctac accgattcca
     1621 tcaccttcaa tgtaagggat aatcggattt tcaggaacgt tgagtttgcc gttttgcagg
     1681 gtgatcttct tgccttgtgc cggaacaact actttacttt ccattcacct ctccttcgag
     1741 cgctactggt ttgctcgtct gccaccacgt tgcatatgcg tttgcgtcct gcgatacgga
     1801 tgctttagag caattttttg ttaatgattt gtaattggct tgtcaatgct accctattgt
     1861 ttgccgtcat gaaagatgcg cgttattagg ctataatgcg gcaattcata atctctgaaa
     1921 ataccatgca aaaaacttct tttagaaatc accaggttaa gcgattcagc tcgcaacgtt
     1981 ctactaggcg taaacctgaa aaccagccca cgcgtgtgat cctgttcaat aaaccctacg
     2041 atgttcttcc gcagttcacc gatgaagccg gacgcaaaac attaaaagaa ttcatcccgg
     2101 ttcagggcgt ttatgcagca ggtcgtcttg accgcgatac cgaagggtta ctggtgctga
     2161 ccaataacgg cgcgttgcag gcgcgtttaa cccagccggg caaacgcacc gggaaaatct
     2221 attatgtgca ggtggaaggt attcccacac aagacgcact tgaagccttg cgcaatggcg
     2281 taaccttaaa tgatggccct accctgcccg ccggcgcgga gctggttgac gaaccagcgt
     2341 ggttatggcc gcggaatcca ccaattcgtg aacgcaaaag tattcccacc agctggctga
     2401 agatcacctt atatgaagga cgtaatcgcc aggtgcgccg catgaccgcc catgttggtt
     2461 tccccacgct gcgactgatt cgttatgcga tgggtgatta cactttggat aaccttgcca
     2521 atggcgaatg gcgagaagca acagaataag gatgcacaat gtttaaaccg cacgttaccg
     2581 ttgcttgcgt agtgcacgca gagggtaaat ttttagtcgt tgaagagacg attaatggta
     2641 aagcgttatg gaaccaacct gccgggcatc tggaagccga tgaaacctta gtggaagctg
     2701 ccgcccgtga gctatgggaa gaaaccggca tcagcgcgca accgcaacac tttattcgta
     2761 tgcatcagtg gattgcgcca gataaaacgc cgtttttgcg tttcctcttt gccattgagc
     2821 ttgagcaaat atgcccgacc cagcctcatg acagcgatat cgactgctgt cgttgggtca
     2881 gcgccgaaga aattttaaag gcgtcaaatc ttcgttcgcc gctggtggcg gaaagtatcc
     2941 gttgttatca aagtgggcaa cgctatccgc tggagatgat tggcgatttt aactggcctt
     3001 ttacaaaggg tgtcatctaa aaggctgcgt gatagaatac gccgccttga agttcaatgt
     3061 cgtgagtgat ccaatgtctg aaaccgcaaa aaaagtaatc gtcggcatgt ccggcggtgt
     3121 cgattcctcc gtttctgcct ggctgttgca acaacaggga tatcaggttg aaggcctgtt
     3181 tatgaagaac tgggaagaag acgacggtga ggaatattgc acagcggcag cggatctggc
     3241 tgatgcccag gctgtctgcg acaagctcgg cattgaactg cacaccgtta actttgctgc
     3301 cgagtactgg gacaatgtct tcgaactgtt ccttgccgaa tataaagccg gtcgcacgcc
     3361 gaatccggat attctgtgca acaaagagat caaatttaaa gctttcctcg aatttgccgc
     3421 cgaagattta ggtgccgatt atatcgctac cggtcattac gtacgtcggg ccgatgtcga
     3481 tggcaagagc cgcctgctgc gtggtctgga cagcaataaa gaccagagct acttccttta
     3541 tacgctcagc catgagcaga ttgcgcaaag cctgttcccg gtcggcgaac tggaaaaacc
     3601 gcaggtgcgt aagattgctg aagatcttgg tctggtcacc gcgaagaaaa aagactctac
     3661 cggcatttgc ttcattggcg aacgtaaatt ccgcgagttc ctgggccgtt atctcccggg
     3721 gcaaccgggc aaaatcatta ccgtcgatgg cgatgaaatt ggtgagcacc aggggctgat
     3781 gtatcacact ctcggtcagc gtaaaggtct gggtatcggt ggcaccaaag aaggcaccga
     3841 agaaccttgg tatgtggtgg acaaagacgt cgaaaacaac attctggttg tcgctcaggg
     3901 ccatgaacac ccgcggctga tgtctgtcgg gttgattgcc cagcagttgc actgggtcga
     3961 tcgcgaacca ttcaccggca ctatgcgttg cacggtaaaa acccgctatc gccagaccga
     4021 tatcccttgc accgtcaagg cgctggacga tgatcgcatt gaagtgattt tcgatgaacc
     4081 ggttgccgcc gtgacgccgg gccagtctgc cgtcttctat aacggtgaag tgtgcctcgg
     4141 tggcggtatt attgagcagc gtctgccgct gccggtctga ttattatctt tacttaaaac
     4201 aaggaagcag tgaacgtggc aaagaattac tatgacatca ccctcgccct ggccggtatt
     4261 tgtcagtcgg cacgcctggt gcaacaactc gctcaccagg ggcattgtga tgccgatgcg
     4321 ctacacgtct cactcaacag tattattgat atgaacccca gctcgacgct ggcggttttt
     4381 ggcggtagcg aagccaacct gcgcgtcggg ctggaaaccc tgctcggcgt gctcaatgcc
     4441 agcagtcgcc agggcttaaa cgccgaatta acccgctaca cactcagctt gatggtgctt
     4501 gagcgcaaac tctcctcagc gaaaggcgcg ctcgacactc tgggcaaccg gatcaacggc
     4561 ctgcaacgcc agctcgaaca cttcgattta cagtccgaaa cgctgatgag cgcgatggct
     4621 gctatctatg ttgatgtgat cagcccgctt ggcccacgca ttcaggtcac cggttcccct
     4681 gctgtactgc aaagcccaca agtgcaggcg aaagttcgcg caaccctgct ggcaggcatt
     4741 cgcgccgccg tgctctggca ccaggtcggc ggcggacgtc tgcaactgat gttttctcgt
     4801 aatcgcctga ccactcaggc aaaacaaatt cttgctcatt taaccccgga gttgtgatct
     4861 atggaattat cctcactgac cgccgtttcc cctgtcgatg gacgctacgg cgataaagtc
     4921 agcgcgctgc gcgggatttt cagcgaatat ggtttgctga aattccgtgt acaagttgaa
     4981 gtacgttggc tgcaaaaact ggccgcgcac gcagcgatca aggaagttcc tgcttttgct
     5041 gccgacgcaa tcggttacct tgatgcaatt gtcgccaatt tcagcgaaga agatgccgca
     5101 cgcatcaaaa ccatcgagcg taccactaac cacgacgtta aagcggttga gtatttcctg
     5161 aaagaaaaag tggcggagat cccggaactg cacgcggttt ctgaattcat ccactttgcc
     5221 tgtacttcgg aagatatcaa taacctctcc cacgcattaa tgctgaaaac cgcgcgtgat
     5281 gaagtgatcc tgccgtactg gcgtcaactg attgatggca ttaaagatct cgccgttcag
     5341 taccgcgata tcccgctgct gtctcgtacc cacggtcagc cagccacgcc gtcaaccatc
     5401 ggtaaagaga tggcaaacgt cgcctaccgt atggagcgcc agtaccgcca gcttaaccag
     5461 gtggagatcc tcggcaaaat caacggcgcg gtcggtaact ataacgccca catcgccgct
     5521 tacccggaag ttgactggca tcagttcagc gaagagttcg tcacctcgct gggtattcaa
     5581 tggaacccgt acactaccca gattgaaccg cacgactaca tcgccgagct gtttgattgc
     5641 gttgcacgct tcaacaccat tctgatcgac tttgatcgtg acgtctgggg ttatatcgcc
     5701 cttaaccact tcaaacagaa aaccattgct ggtgagattg gttcttccac catgccgcat
     5761 aaagttaacc cgatcgactt cgagaactcc gaagggaatc tgggcctttc caacgcggta
     5821 ttacagcatc tggcaagcaa actgccggtt tcccgctggc agcgtgacct gaccgactcc
     5881 accgtgctgc gtaacctcgg cgtgggtatc ggttatgcgc tgattgcgta tcaatccacc
     5941 ctgaaaggcg tgagcaaact ggaagtgaac cgtgaccatc tgctggatga gctggatcac
     6001 aactgggaag tgctggctga gccaatccag acagttatgc gtcgctatgg catcgaaaaa
     6061 ccgtacgaga agctgaaaga gctgactcgt ggtaagcgcg ttgacgccga aggcatgaag
     6121 cagtttatcg acggtctggc gttgccagaa gaagagaaag cccgcctgaa agcgatgacg
     6181 ccggcaaact acattggtcg cgccatcacc atggttgatg agctgaaata aactcgtatc
     6241 agtgccggat ggcgatgctg tccggcctgc ttattaagat tatccgcttt ttattttttc
     6301 actttacctc ccctccccgc tggtttattt aatgtttacc cccataacca cataatcgcg
     6361 ttacactatt ttaataatta agacagggag aaataaaaat gcgcgtactg gttgttgaag
     6421 acaatgcgtt gttacgtcac caccttaaag ttcagattca ggatgctggt catcaggtcg
     6481 atgatgcaga agatgccaaa gaagccgatt attatctcaa tgaacattta ccggatattg
     6541 cgattgtcga tctcggattg ccagacgagg acggtctgtc actgattcgc cgctggcgta
     6601 gcaatgatgt ttcactgccg attctggtat taaccgcccg tgaaagctgg caggacaaag
     6661 tcgaagtatt aagtgctggt gctgatgatt acgtgactaa accatttcat attgaagagg
     6721 tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg tctggcttca caggtcattt
     6781 cgctaccacc gtttcaggtt gatctctctc gccgtgaatt atctattaat gacgaagtga
     6841 tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt gatacgcaat aatggcaaag
     6901 tggtgagcaa agattcgtta atgctccaac tctatccgga tgcggagctg cgggaaagcc
     6961 ataccattga tgtactgatg ggacgtctgc gcaaaaaaat tcaggcacaa tatccgcaag
     7021 aagtgattac taccgttcgc ggccagggct atttgttcga attgcgctga tgaaaaaatt
     7081 actgcgtctt tttttcccgc tctcgctgcg ggtacgtttt ctgttggcaa cggctgcggt
     7141 agtactggtg ctttcgcttg cctacggaat ggtcgcgctg atcggttata gcgtcagttt
     7201 cgataaaact acgtttcggc tgttacgtgg cgagagcaat ctgttctata cccttgcgaa
     7261 gtgggaaaac aataagttgc atgtcgagtt acccgaaaat atcgacaagc aaagccccac
     7321 catgacgcta atttatgatg agaacgggca gcttttatgg gcgcaacgtg acgtgccctg
     7381 gctgatgaag atgatccagc ctgactggct gaaatcgaat ggttttcatg aaattgaagc
     7441 ggatgttaac gataccagcc tcttgctgag tggagatcat tcgatacagc aacagttgca
     7501 ggaagtgcgg gaagatgatg acgacgcgga gatgacccac tcggtggcag taaacgtcta
     7561 cccggcaaca tcgcggatgc caaaattaac cattgtggtg gtggatacca ttccggtgga
     7621 gctaaaaagt tcctatatgg tctggagctg gtttatctat gtgctctcag ccaatctgct
     7681 gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt ttacgcccca tcgaagccct
     7741 ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa ttgctcaatc cagccacaac
     7801 gcgagaactg accagtctgg tacgaaacct gaaccgattg ttaaaaagtg aacgcgaacg
     7861 ttacgacaaa taccgtacga cgctcaccga cctgacccat agtctgaaaa cgccactggc
     7921 ggtgctgcaa agtacgctgc gttctctgcg tagtgaaaag atgagcgtca gtgatgctga
     7981 gccggtaatg ctggagcaaa tcagccgcat ttcacagcaa attggctact acctgcatcg
     8041 tgccagtatg cgcggcggga cattgctcag ccgcgagctg catccggtcg ccccactgct
     8101 ggacaatctc acctcagcgc tgaacaaagt gtatcaacgc aaaggggtca atatctctct
     8161 cgatatttcg ccagagatca gctttgtcgg tgagcagaac gattttgtcg aggtgatggg
     8221 caacgtgctg gataatgcct gtaaatattg cctcgagttt gtcgaaattt ctgcaaggca
     8281 aaccgacgag catctctata ttgtggtcga ggatgatggc cccggtattc cattaagcaa
     8341 gcgagaggtc attttcgacc gtggtcaacg ggttgatact ttacgccctg ggcaaggtgt
     8401 agggctggcg gtagcccgcg aaatcaccga gcaatatgag ggtaaaatcg tcgccggaga
     8461 gagcatgctg ggcggtgcgc ggatggaggt gatttttggt cgccagcatt ctgcgccgaa
     8521 agatgaataa atatgtccat acttcacgca ttacgttaag catccgttat aatcggttgc
     8581 agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact ggcccgattt
     8641 tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta ataattttat
     8701 tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag ttgacagtcg
     8761 actggtcagt caccaggatg gcaaatggca ggtcagccac ggcccgttcg aaagctacga
     8821 tcatctcggt gaaaccaact ggtcattact ggtacaggca gtgaaccact ggcatgagcc
     8881 gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg atgatctgat
     8941 gatttctttt tctgtacctg gcggcggcgt cggcccgcat ctcgatcagt acgacgtgtt
     9001 tatcattcag ggtaccggac gtcgtcgctg gcgagtgggc gaaaagctgc aaatgaaaca
     9061 gcactgccca catccggatc tgttacaggt cgatccgttc gaagccatca tcgatgaaga
     9121 gctggagcct ggtgatattc tttatattcc gccaggattc ccgcatgaag gctacgcgct
     9181 ggaaaatgcg atgaactatt ccgtgggctt tcgcgcgcca aatacgcggg aactgattag
     9241 tggatttgcc gattatgtgc tgcaacgtga actgggcggc aactactaca gcgatcctga
     9301 tgttccacct cgcgctcatc ctgcggatgt tctgcctcaa gagatggata aactgcgtga
     9361 gatgatgctc gaattgatca accagccgga acactttaag caatggtttg gcgagtttat
     9421 atcccagtca cgtcatgaac tggatatcgc gccgccagaa ccgccttatc agccagatga
     9481 aatctacgat gcgctgaaac aaggtgatgt gctggtgcgc ctgggtggtc tgcgcgtatt
     9541 gcgcattggc gacgacgtgt atgccaatgg tgagaagatc gattccccgc accgtccggc
     9601 actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg atgcgctgga
     9661 agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt ggttcttcga
     9721 agggtaagtt tggatttaag ccggatgcgg catcacatgc cgcatccctt ttcgcttact
     9781 tccgttgcgc cgttaactcg gcaatacgga cgatcacctg caccgctttt tccatacctt
     9841 ccagagtcac aaactcatgc ttaccatgat agttgtaacc gccagtgaac aagttcgggc
     9901 acggtaatcc cataaacgac aactgcgcgc cgtcggtacc accgcggatc ggtttcagtt
     9961 ccggttcaat atcgcagtca cgcatcgcct gctgggcgat atcgagaata tgcggatgct
    10021 caaccacttt ctctcgcata ttgtagtaac tgtcttcaat caccagttca atgtagcaat
    10081 caggatgtaa ccctttgccc acttttttgg cgatctccat cattttacgt ttacgcgctt
    10141 caaactgttt acggtcgaaa tcacggatga tgtagtgcat atcggcccgt tcaacggtgc
    10201 ctttcatgct cgccaggtga tagaaacctt catagccttc tgtcatttcc gggctttcat
    10261 ccgccggaac ttccgcatga atacgtgccg ccagcgacag cgcatttacc atcactcctt
    10321 tcgccgtgcc cggatgaaca ttgttaccga caattttgat attgaccgat gcggcgttga
    10381 agttttcaaa ctccagttcg cctacgccac caccgtcaac agtgtaagcc cagcgggcat
    10441 cgaaggcatc aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc ggggtaaagg
    10501 cgacgcgaat atcaccatgc ggaatgtttt tctgttgcaa taccgccagc gcggtcatga
    10561 tttctgcaat acctgctttg tcatcggcac ctaacaaggt tttaccatcg gtggtaatca
    10621 acgtctgacc cagtagctga tgcagcaccg ggaacataac cggtgataaa acttcatcgc
    10681 cgatacccag cgcaatatcg ccaccgcgat agttttcaac aatttgcgga ttaacatttt
    10741 tgccgctgca atccggtgag gtatccacat gagaaataaa gccaatcgcc gggatatcgc
    10801 cagggacgtt agccggtaac gtcgccatca aagtgccctt ctcacttaag gtcacattga
    10861 taagccccat ttcttcgagc tgctctttca gcagatgcaa taacttccat tggccttccg
    10921 tgctgggaac ctgtctcacc cctgcttttg attgggtatc cagagatacg tagttcaaaa
    10981 atcgctcaag tagtttatcc atgtagtcac cctcactttt tgtgacaaca ttattaagaa
    11041 gcaagaaaag acaaatattg cgtcaggtca cttttacccc tgcaagcggg aatatttatc
    11101 agcattacct taatgaatat aaagctaagc cagtaattca caacaaagat tggcgattca
    11161 agcggtttgc cgtagaatta ccgccctcat taagagtcac caaggtggtt aaccacaaac
    11221 cccgcatcgg taagccatcc gttgcgttta catgggacag agtaaaaaat tgaataaaca
    11281 accgagttcg ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa
    11341 agaggtcatt ccccagctgg atctgactat caacaatggc gagttcctca cgctgcttgg
    11401 cccttctggc tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg aaactgttga
    11461 ttccggacgc atcatgctgg ataacgagga catcacccac gttccggcgg aaaaccgcta
    11521 tgtgaacact gttttccaaa gctacgcact tttcccccac atgaccgtgt tcgaaaatgt
    11581 ggcctttggg ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga
    11641 agccctgcgg atggtgcaac tggaaacctt cgctcaacgt aaaccgcatc agctctctgg
    11701 tggtcaacag caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc gtctgttgtt
    11761 gctggatgag tcgctctcag cgctggatta caaactgcgt aagcaaatgc agaacgagct
    11821 gaaagcgtta cagcgtaagc ttggtattac cttcgtcttt gtcactcacg atcaggaaga
    11881 agcgctcacc atgtcagaca ggattgtggt gatgcgcgag ggtcgtattg agcaagatgg
    11941 tacaccgcgc gaaatttacg aagagccgaa aaacctgttt gttgccggct tcattggcga
    12001 aatcaatatg tttaacgcca ctgtcatcga acgtctcgac gagcagcgcg tacgcgccaa
    12061 cgttgaaggc cgcgaatgta atatctacgt taacttcgcc gttgaaccgg ggcaaaaact
    12121 gcatgttctg ctgcgcccgg aagacttacg tgttgaagag attaacgacg acaaccacgc
    12181 tgaagggctg attggttacg ttcgtgagcg taactacaaa ggcatgacgc tggagtcggt
    12241 tgttgaactg gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg aagacgaccc
    12301 tgactttgac cactctctcg accaaaaaat ggccattaat tgggtagaaa gctgggaggt
    12361 cgtactggct gatgaagaac acaagtaagt tccagaatgt agtgattgtc actattgtcg
    12421 gttggcttgt gttgtttgtc tttctgccca acctgatgat cattggcacc agctttttga
    12481 cccgcgacga cgccagtttc gtcaaaatgg tctttacgct ggataactac acgcgtctgc
    12541 tcgatccgct ctattttgaa gtgctattgc actcgctgaa tatggcgctg atcgccaccc
    12601 tcgcctgcct ggtgctgggc tacccgtttg cctggtttct ggcgaagctg ccacacaagg
    12661 tgcgtccgct gctgctgttt ctgctgattg ttcccttctg gaccaactca ctgattcgca
    12721 tctacgggct gaaaattttc ctcagcacca aaggctatct caacgagttt ttgctctggc
    12781 tgggcgttat cgacacacca attcgtatca tgttcacgcc cagtgcggtg attatcggtc
    12841 tggtttacat tctgctaccg tttatggtga tgccgctgta ctccagtatc gaaaaactgg
    12901 ataagccgtt gcttgaggcg gcgcgcgatc tcggtgccag caagttacag acttttatcc
    12961 gtatcattat tccactgacg atgccgggaa ttattgccgg atgtctgctg gtgatgctgc
    13021 cagcgatggg cctgttctat gtatccgacc tgatgggcgg tgcgaaaaac ctgctgatcg
    13081 gtaacgtcat caaggtccag ttcctcaata ttcgtgactg gccgtttggt gcagccacca
    13141 gcattacgct gactatcgta atgggcctga tgttgctggt ttactggcgc gcttctcgtc
    13201 tgctgaataa aaaggtggaa ctcgaatgat cggtcgactg cttcgcggcg gttttatgac
    13261 cgctatctac gcgtacctgt atatcccaat cattattttg attgtgaact cctttaacag
    13321 ctcgcgcttt ggcatcaact ggcagggttt taccaccaaa tggtatagcc tgctgatgaa
    13381 caacgacagc ctgttacagg cagcgcagca ttcactaaca atggcggtgt tttcggcgac
    13441 atttgctacg cttatcggtt cactgacggc agttgcgctg taccgttatc gctttcgtgg
    13501 taagccgttc gttagcggaa tgctgtttgt ggtgatgatg tcaccagata tcgtgatggc
    13561 gatttctctg ctggtgctgt ttatgctgct gggtattcag cttggcttct ggtcgctgct
    13621 gttctcgcat atcaccttct gcctgccgtt tgtggtggtg acggtgtatt cgcgcctgaa
    13681 aggttttgac gtgcggatgc tggaagcggc gaaagatctc ggtgccagtg aatttaccat
    13741 tctgcgaaaa atcattctgc cactggcaat gccagcggtg gcggcgggct gggtgttaag
    13801 ctttaccctg tcgatggacg atgtggtagt ttcttcgttc gtcaccggac caagttatga
    13861 aattctgccg ttaaaaattt attcgatggt caaagtcggc gtatcgccgg aagttaacgc
    13921 gctggcaacc atattactgg tgctgtcgct ggtgatggtt attgccagcc agcttattgc
    13981 tcgtgataaa acgaaaggta acacagggga cgttaaatga aaaaatggtc acgccacctg
    14041 ctcgcggcgg gtgctctggc actgggcatg agcgccgctc acgccgatga caacaacacg
    14101 ctgtatttct acaactggac cgagtacgtg ccgccaggac tgcttgaaca gttcaccaaa
    14161 gaaaccggta ttaaggctat ctattcgact tacgagtcga acgaaaccat gtacgccaag
    14221 ctgaaaacgt acaaagacgg tgcctatgac ctggtggttc cttcaaccta ttacgtcgat
    14281 aaaatgcgta aagaagggat gatccagaag atcgacaagt cgaagttaac gaacttcagc
    14341 aatctcgatc cagacatgct caacaagccg tttgacccga ataacgacta ctccattccg
    14401 tatatctggg gtgcgacggc gattggcgtt aacggtgatg cggtggatcc taaatctgtc
    14461 accagttggg cagatctgtg gaagccagag tacaaaggta gcctgctgct gaccgacgat
    14521 gcccgtgaag tgttccagat ggcgctgcgc aaactgggct actccggtaa caccaccgat
    14581 ccgaaagaga ttgaagctgc atataacgag ctgaaaaaac tgatgccaaa cgtcgcagcg
    14641 tttaactccg ataacccggc taacccgtac atggaaggcg aagttaacct cggcatgatc
    14701 tggaacggct ctgctttcgt tgcacgccag gcgggtacgc caattgacgt ggtgtggccg
    14761 aaagaaggcg gcattttctg gatggacagc ctggcgatcc cggcaaatgc caaaaacaaa
    14821 gaaggcgcgc tgaaattgat caacttcctg ctgcgcccgg atgtggcaaa acaggttgcc
    14881 gaaactatcg gttatccaac gccaaacctt gcggcgcgta agctgttaag cccggaagtg
    14941 gcgaacgata aaacgctcta cccggatgct gaaaccatta aaaatggtga atggcagaat
    15001 gacgttggcg cagccagcag catttatgaa gagtattatc agaagctgaa agcaggacgt
    15061 taattcagca aacgggcggt ggtattaccg cccgtttttt agttagcaag gatgtggcta
    15121 atgagtcagg acagtaaagt tttatcccgc tctttcgtcg gtattgtagt ccccttgtag
    15181 gcctgataag cgtagcgcat caggcaatgc ttcctgcttt ataatccctt cagcaacttt
    15241 tcaacaaact caggcaccac ctggcttgcc gggccgtaat atttctcggc aaattcatta
    15301 ccgacctgac ttggttcgag attcagttcc acggtgtgcg cgccatgcag tttcgcttcg
    15361 tgaacaaacc cagccgccgg ataaacatgc ccggaagtac caatggcaat gaaaatatca
    15421 gccatcgaca acgccatata aatttcatcc atgccgagtg gcatttcgcc aaaccaaacg
    15481 acgtgcgggc gcaagggtgc cgggaactgg caacaatggc atttatcttc tggcgtaacg
    15541 tctccggtcc agtcgagaac ctgaccactt tgcgaacaac gaactttcag caattcccca
    15601 tgcatatgaa tgacattggt attacctgcg cgttcatgca ggttgtctat attctgcgtt
    15661 accagcaaaa agcgatcgcc gagggcgtct tgcagtttag ccagcgcaag atgcgcggcg
    15721 ttaggctgaa tttctggctg ctgcagctgt cgacgacggg cgttataaaa cgcttgcacc
    15781 agttcaggat cgcgatcgaa accttccggc gttgccacat cttcaacccg atgttcttcc
    15841 cacaggccat ctgcggcgcg aaaggtacga atacctgatt ccgcagaaat tcctgcccct
    15901 gtcagtacga gtactcttgg tttttccatt gcttccggca ccactttatc tctgaaaaaa
    15961 atacgctgac gcaaacgctc gcgcaggcgg cgtttatttt tacgaaaacg acttaaccga
    16021 tgaccccgac gcgacagcat agcaacctct gttgtttaat cggttagatg taggaaggcc
    16081 gcaccacgca ttcctcccgc atcaccgtgg cgcgcgcgtt caatgcgcgg aacacgagct
    16141 acaggtaaga gatgacgagg cagcctgtcc gccagttgcg ttgtgattgc cgggaaattc
    16201 gataagccac caccaatgac gaccaggtca gagtcaacga tggtcaggat atttcccaga
    16261 caaaccgcta ataaatccag ataacgctca acgtgcgccc ttgcctgctc atcgccttga
    16321 tcataaagcg caataatttc gggagcctgc aacggttgat gataatagtg ttgccagagc
    16381 cacgcaaagc cccgacctga tagataattt tcgatacaac caatttgacc acagccgcag
    16441 cggcgtaacg ggaaatccag ccccatcatg gttaacgcat caaccggcag acgcatatgg
    16501 ccaaactcgc cggtaatgta gcttttaccg gtaatcggtt taccgttgaa aatcagcccg
    16561 ccgccaacgc cggtgccgag aattaacccc atcaccagtg gatattgcgt aaattcatca
    16621 tcccaggctt ctgaaagggc aaaacagttg gcatcgttat caaggcgtac atcgcgatca
    16681 agacgtgcgc tcaggtcggc acgcagcggt ttaccgctgg cagcggggac attggcggca
    16741 tacagcgtac catcttctgt ttccggcatt cccggaatac cgatgccaac agaaccttta
    16801 cagccaaaac gttggtcagc ttcggctacc agctcgcata ctgcatctaa aaatgcgtca
    16861 tagctgtcac gcggtgtcgg cacccgcttt tcccactgca actgccgacc gctatcaaac
    16921 acgccaagcg caatttttgt tccaccaata tcaaacccgt aatacattgc cgctccttgt
    16981 tttaatgtac tgcctttact ggccgctaag gactcgcgca gggtcaatat tactggcgcg
    17041 ccgcgccgga taccaacttg ccaaaagact cagcaacaat gctgtgacca gtacgtagaa
    17101 gacgtccagc cagtgcaatt ccgatggcag gaagtcaata aaatagatat cgctggagag
    17161 gaactgatga ccgatcagct tttcaatccg ctcaataatc ggggtaagtt gcagtgaaac
    17221 aactacgccg ataatcacac cacacaggct accgaacagc cctgccagca atccatacca
    17281 gacaaagatg gcgcgaatta aaccatcttt cgcccccagc gttcttaata ctgcgatatc
    17341 gccactcttg tctttcaccg ccatcactaa ggtggagacg atgttgaaac aggccacgcc
    17401 aatcaccagt accatcgcca gatacataat ggcgcggatc atctggatat cgcgatacat
    17461 atagccgtaa gtaccaatcc aacttttaat ataaacatag ctgttggtca cttcacccgc
    17521 atcgcgtacc agcttattgg cgttgaaaac atccgtcatt ttaagggcaa tacctgacac
    17581 gctggaaccc atatcaagat attgttgggc atccgccagc gggatcatgg caaaactgtg
    17641 atcgagttga ccactcaact gcaaaatacc ggcaatgtgc aaacgcacac gttttggctg
    17701 catcagttta tgctcgggat tcgagttggg aatcatgatc gacacccaat cgccctgctt
    17761 cactttcagc gcatccgcca cgcctttgcc gatgataatt tgctgttcgc ccgctttaaa
    17821 attgcgccag gcatccccct gaacaaacga gggtaatgcg ctcagacgct gttcctgttg
    17881 cgggttaacg cccttcacct gaattgcgcg cagattggta ccactttcta ccagtccggt
    17941 gaaattgata tacggcgcag cggcggcaat acccggcact ttttgcacgt tatccagtgc
    18001 ttcctgccag ttagtccatg gctgatttac cgcctcaatt tcaccgtgcg gtaccacagc
    18061 aagaatgcgg ttattcagtt cgcgttcaaa gccgttcatc gcgcttaagc cgacgatcaa
    18121 taccgccacg ccgagggcaa tgccaatggt agaaatgaca gagattagcg acaccatgcc
    18181 gccgcgtcgc cgtccgcgac taaaacgcag gccaatcaat aacgataaag gcatcgccat
    18241 tactccgccc ccatcaggct cagttccgcc gtcagacgtc catcacgcat ttccagttgg
    18301 cggctcatac gtttcgccag ttgcaggtcg tgagtaacca ccaggaaggc ggtgccctgc
    18361 aagcgattca attccccaag caactggaag atgctgtcgg cgttacgcgc atcgaggtta
    18421 ccggtaggct catccgcaag taccagacgc ggattattga ccagcgcacg ggcaatcgcc
    18481 acacgctggc gttcgccgcc agaaagttca gatgggcggt gattcgcacg atgctccagc
    18541 cccaccgctt ttaacatctc cagcgcacgg ctgttgattt cagcgggctt tttcttgcca
    18601 atcagcagtg gcatagccac gttttccagg gcagtaaaat ccggcagcag gtggtgaaac
    18661 tgataaataa agcccagctt ctggttgcgc agttctgctt tcgccgccga agacagtttg
    18721 ctcatcggtt gaccgttaaa gatcacatcg ccggaggttg gtgtatccag cccgcccagc
    18781 aggtgcagca aggtactttt accggaacca gagctaccga cgatcgccat catttcacct
    18841 tcgccgacgc tgaaactgac attgtgcagt acatcggttt gcacactgcc ttcctgatag
    18901 cgtttgcaca ggttgtcgca ttgcaacagg atcttattca taacgtaaag cctcagcggg
    18961 ttgagtggcg gcagcgcgcc atgaagggta aagcgtagac agcagcgcga tagccatcgc
    19021 caccagcgca ataacaatga cctgtaaagg ttcgatagcg accggcagcg ccgcgccatc
    19081 aagcaggacg ccgattatcg gcatcagatt atttaactgg ctggcaagca gtgcgccaag
    19141 cgccgctccg aggatcgcac cgataatccc ggcgctggcc ccttgcacca taaagaccat
    19201 catgatttgt cgcggagtta agccttgcgt ttgcaggatc gcgacttcgc cctgcttctc
    19261 cattaccatc agccctagtg aggtaataat gttaaacgcc gcaacggcga caatcaggct
    19321 cagcagcaag cccatcatat ttttttccat gcgtacggcc tggaacagct cgcctttacg
    19381 gtcacgccag tcctgccatt tgctgccttc aggcagtttt tgctgactta aagagtcaac
    19441 tttcagcggc tcatccagcc acaaacgcca gccggtaata ttgcctgccg gataacgcat
    19501 cagacgcgag gcatcctcaa tattcaccag catttcatag ccatcgactt cactgttggc
    19561 ggcgaaattg ccaatcacat tgaacaggcg ctggcttgga atacgcccca tcggcgtgaa
    19621 ctggctggca gatggtacca tcacgcggat ttgatcaccg cgattaacgc ctagctgtga
    19681 ggcaagttgt tcgccgagga tgacattata tttccccggc tcgaggtcag tttgtttcac
    19741 attgaccaga tacggcgtaa gtggatcttt ttgcgccggg tcgataccga gcatcacccc
    19801 gaccgccacg ctgcgcgcgc tttgcagtac cacatcaccg gtagtaatag gtgcgacgcg
    19861 attaacgccg tccagtttga ctgccgtttc cgggagttgc tgcgggttaa gagagccatg
    19921 ctcagaagag agaattgcct gtggcatcag gccaaggatg ttgttttgca gctcgcgctc
    19981 aaagccgttc atcactgaca atactgtgac cagcgccatc accccgaggg taatgccgat
    20041 ggtagaaagc caggagacga aacgaccgaa gcgatccgct gcacgcccac gcatgtaacg
    20101 caggccaata aatagagcga caggttggta catgaaatcc gtctggttgc tgtagcaaag
    20161 acacgagtat ataagcgaaa gcaagatggg taaacgtctg aactgtaagt gatcgtcagg
    20221 gctaatctga aaagcccccg taaatcaatc ggctaaattg ccatgtttta tcatcaatct
    20281 tgccgcctga tcttaatctg aaaaatcatc caatacagac agatacccat tacacctcgg
    20341 gtcataaccc gcaggatcaa ccgcatgtaa attaaaaaag agcgtgttgt cctgatgaaa
    20401 caaaaagagc tatggattaa ccagatcaaa gggttatgta tttgtctggt ggtgatttat
    20461 cactcggtca ttacctttta tccgcatatg accacttttc agcatccgtt atcggaagtc
    20521 ctgagtaaat gctggatcta tttcaatctt taccttgcac cctttcgtat gccggttttt
    20581 ttctttatat ccggctattt gattcgccgc tatatcgaca gcgtaccgtg gggaaattgt
    20641 ctcgataaac ggatctggaa catcttctgg gtgctggcgc tttggggcgt ggtgcagtgg
    20701 ctggcgttaa gtgcgctgaa ccagtggctg gcacctgagc gcgatttaag caatgcctcc
    20761 aatgccgctt atgccgattc caccggtgag ttcctgcacg ggatgatcac cgccagcacc
    20821 agcttgtggt atctgtatgc gttaattgtc tatttcgtgg tatgtaaaat ttttagccgt
    20881 ctggcgctgc cactattcgc cttgtttgta ctgctgagtg tggcggttaa tttcgttccc
    20941 acgccgtggt ggggaatgaa cagtgtgatc cgcaatttgc cttattacag ccttggcgca
    21001 tggtttggcg caacaataat gacctgtgtt aaagaggtgc cgttgcgccg ccatctgctg
    21061 atggcttctt tgcttaccgt tctggcggtc ggtgcctggt tgtttactat ctcgctgctg
    21121 ttgtcgctgg tatcgattgt ggtgatcatg aagctgtttt atcagtacga gcaacgattc
    21181 ggtatgcgct ccaccagcct gctgaatgtg attggctcca acaccattgc tatctacacc
    21241 acccatcgca ttctggttga aatattcagc ttaactctgc ttgcgcaaat gaacgcagca
    21301 cgctggtcgc cgcaagtcga actgacactc ctgctggttt atccctttgt cagtttgttc
    21361 atctgtactg ttgcgggctt gctggtaaga aaactttcac agcgtgcatt cagcgatctg
    21421 ttgttctccc cgccttcact gcccgctgcc gtcagttact ctcgttaaac catcgcccca
    21481 gccggggcga ttcaatttgc taaaccatgt cgattacgga taatgaacag cattgcttat
    21541 cagagatatg cccccatatg ttgaggcata tcctaacgag aatctgacaa ccgttatgcc
    21601 tgaacaatat cgttatacac tgcccgtcaa agcgggtgag cagcgtctgc tgggcgagtt
    21661 aaccggcgca gcctgtgcaa cgctggtagc ggaaattgcc gaacgtcacg ccggtccggt
    21721 ggtactcatt gcaccagata tgcaaaatgc tctgcgtttg catgatgaaa tcagccagtt
    21781 caccgatcag atggtgatga atctggcgga ctgggaaact cttccctacg acagtttttc
    21841 gcctcatcag gacattatct cctcgcgcct ttccaccctt taccagctac cgacgatgca
    21901 gcgtggcgta ctgattgttc cggtgaatac gcttatgcag cgcgtttgcc cacacagttt
    21961 tctccacggt catgcgctgg tgatgaaaaa aggtcagcgc ctgtcacgag atgcattacg
    22021 aacccaactg gacagcgccg gttatcgcca tgttgaccag gtgatggagc acggcgaata
    22081 cgccacgcgc ggcgcgttgc tggatctctt cccgatgggg agtgagctgc cttatcgtct
    22141 tgatttcttt gatgatgaaa tcgacagcct gcgggtgttt gacgtcgaca gccagcgcac
    22201 gctggaggaa gtagaagcga tcaatctgct gcccgcgcac gaatttccga ccgataaagc
    22261 ggcaattgaa ctgttccgca gccagtggcg cgataccttc gaagtgaagc gcgatccgga
    22321 acatatttac cagcaagtga gtaaaggcac attacctgcc gggatcgagt actggcagcc
    22381 attgttcttc agcgaaccac tgccgccgct gttcagttat ttccctgcca ataccttgct
    22441 cgtgaatact ggcgatctgg aaaccagtgc cgaacgtttc caggctgaca cgctggcgcg
    22501 ttttgagaat cgcggcgtcg atccgatgcg cccgctgttg ccaccacaat cgctctggct
    22561 gcgggtggac gagctcttct cagagctgaa aaactggccc cgggtgcagc taaaaactga
    22621 acatttaccg acaaaagccg cgaatgccaa tttaggtttc cagaaactgc cagacctggc
    22681 cgttcaggca caacaaaaag cgccgctgga tgcgctgcgt aagttcctcg agactttcga
    22741 cggtccggtg gtgttctcgg tagaaagtga aggtcgccgt gaagcgctgg gtgaactgct
    22801 cgcacgaatt aaaattgctc cgcaacgcat tatgcgtctt gatgaagcca gcgaccgtgg
    22861 gcgttatctg atgattggcg ctgccgaaca tggttttgtc gataaggtgc gtaatctggc
    22921 gctgatctgc gaaagcgatc tgctcggtga acgtgttgcc cgccgtcgtc aggattctcg
    22981 ccgcaccatc aaccccgata cactgatccg taaccttgcg gagctgcata ttggtcagcc
    23041 ggtggtccat ctggagcacg gtgtcgggcg ctatgccgga atgaccacgc tcgaagcggg
    23101 cggcattact ggcgagtatt tgatgctcac ctatgccaac gacgccaaac tgtatgttcc
    23161 ggtgtcgtca ctgcatctga ttagccgtta cgcaggtggc gcggaagaaa acgccccgct
    23221 gcataaactt ggcggcgatg cgtggtcacg cgcgcggcag aaagcggcgg aaaaagtgcg
    23281 tgatgtggcg gcggaattgc tggatatcta cgcgcaacgc gccgccaaag agggcttcgc
    23341 gtttaaacac gatcgtgagc agtatcagtt gttctgcgac agcttcccgt ttgaaaccac
    23401 gccggatcag gcgcaggcca ttaatgcggt acttagcgac atgtgtcagc cgctggcaat
    23461 ggatcgtctg gtgtgcggcg atgttggctt tggtaaaaca gaagtggcga tgcgcgccgc
    23521 tttcctggca gtagataacc acaagcaggt ggcggtgttg gtgcctacca cccttctcgc
    23581 gcagcagcat tacgacaact tccgcgaccg tttcgccaac tggccggtac gtatcgaaat
    23641 gatctcccgt ttccgcagcg ccaaagagca gacgcaaatc cttgcggaag tggcggaagg
    23701 gaaaatcgat attctgatcg gtacgcacaa actgctgcaa agtgacgtca agtttaaaga
    23761 tttaggcctg ctgattgtcg atgaagaaca ccgcttcggg gtgcgtcata aagagcgcat
    23821 taaagcgatg cgcgcgaacg tggatattct gacgcttact gcaacgccga tcccacgcac
    23881 gctgaatatg gcaatgagcg gaatgcgtga cctgtcgatt atcgccacgc cgcccgcccg
    23941 tcgtctggca gttaaaacct ttgtccgtga gtatgacagc ctagtggtcc gggaggcgat
    24001 cctgcgtgaa attttgcgcg gggggcaggt ttattatctc tacaatgatg tggaaaacat
    24061 ccagaaagct gccgaacggc tggcagaact ggtgccagaa gcgcggatcg ccatcggtca
    24121 cgggcagatg cgcgagcgcg aactggaacg ggtgatgaat gatttccatc atcaacgttt
    24181 caacgtgctg gtttgtacaa ccattatcga aaccgggatc gacatcccga cagccaacac
    24241 cattatcatt gaacgcgcgg atcacttcgg tctggcgcag ctgcaccagt tacgcggtcg
    24301 cgtcggacgt tcgcatcatc aggcatatgc atggttgctg acaccgcatc caaaagcgat
    24361 gactaccgat gcacaaaaac gtcttgaagc gattgcctcg ctggaagatc tcggggcagg
    24421 ttttgcgctg gcaacgcacg atctggagat tcgcggcgcg ggtgaactgc ttggcgaaga
    24481 acaaagcggc tcaatggaaa ccatcggttt ctcgctgtat atggagttgc tggaaaacgc
    24541 cgtcgatgca ctgaaagccg gacgcgagcc gtcgctggaa gatctcacca gccagcaaac
    24601 agaagtcgag ctgcggatgc cgtcgctatt gccagatgat ttcattcctg acgtgaatac
    24661 gcgtttgtcg ttctataaac gtattgccag cgccaaaacg gaaaacgaac tggaagagat
    24721 caaagtcgag cttatcgatc gcttcggcct gctgccagat ccggcgcgta ccctgctgga
    24781 tattgcccgt ctgcgccagc aagcgcagaa actggggatc aggaagctgg aaggtaatga
    24841 gaaaggcggg gtgatcgaat ttgccgagaa gaatcacgtt aatccggcct ggttgattgg
    24901 tttgctgcaa aaacagccgc agcattaccg ccttgatggt ccgacgcgcc tgaaatttat
    24961 tcaggatttg agtgagcgga aaacgcgtat cgaatgggta cgccagttta tgcgtgaact
    25021 ggaagagaac gcgatcgctt aatggtaaat cgggggcgtt tctgcgcccc catctgcaac
    25081 atttacaaat tctttgcact tccctgcact atccgacact gtcaccatcc ataattcagg
    25141 cttatctgtt tattacaata accttatatt tattattgat ttttggtgat gatcaaaacg
    25201 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct accggcaaaa
    25261 gccaacacct agccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    25321 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    25381 ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    25441 ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    25501 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    25561 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    25621 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    25681 cagggaattg agttgcctac ggtagtgccg gctggaccgg ataacccaat gggccatcat
    25741 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    25801 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    25861 ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    25921 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    25981 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    26041 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    26101 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    26161 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    26221 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    26281 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    26341 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    26401 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    26461 tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa
    26521 ttacttaacg atcgataact aaatgataga tgtga
//
LOCUS       DAZSBF010000038        25647 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZSBF010000038 DAZSBF010000000
VERSION     DAZSBF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.038"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHXWD_001207"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="ACHXWD_001207"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR7620178.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHXWD_001208"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="ACHXWD_001208"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR7620179.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHXWD_001209"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="ACHXWD_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR7620180.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHXWD_001210"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="ACHXWD_001210"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR7620181.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="ACHXWD_001211"
     CDS             complement(5499..5696)
                     /locus_tag="ACHXWD_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR7620182.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHXWD_001212"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="ACHXWD_001212"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR7620183.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHXWD_001213"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="ACHXWD_001213"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR7620184.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHXWD_001214"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="ACHXWD_001214"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR7620185.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHXWD_001215"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="ACHXWD_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR7620186.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHXWD_001216"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="ACHXWD_001216"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR7620187.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHXWD_001217"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="ACHXWD_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR7620188.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHXWD_001218"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="ACHXWD_001218"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR7620189.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHXWD_001219"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="ACHXWD_001219"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR7620190.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHXWD_001220"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="ACHXWD_001220"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR7620191.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="ACHXWD_001221"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="ACHXWD_001221"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR7620192.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="ACHXWD_001222"
     CDS             complement(17138..17191)
                     /locus_tag="ACHXWD_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620193.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="ACHXWD_001223"
     tRNA            complement(17425..17500)
                     /locus_tag="ACHXWD_001223"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="ACHXWD_001224"
     tRNA            complement(17536..17611)
                     /locus_tag="ACHXWD_001224"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="ACHXWD_001225"
     tRNA            complement(17769..17844)
                     /locus_tag="ACHXWD_001225"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="ACHXWD_001226"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="ACHXWD_001226"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR7620194.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="ACHXWD_001227"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="ACHXWD_001227"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR7620195.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="asd"
                     /locus_tag="ACHXWD_001228"
     CDS             19844..20812
                     /gene="asd"
                     /locus_tag="ACHXWD_001228"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR7620196.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="ACHXWD_001229"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="ACHXWD_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR7620197.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="ACHXWD_001230"
     CDS             complement(24308..>25647)
                     /gene="yjeM"
                     /locus_tag="ACHXWD_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR7620198.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5934 a         7194 c         6499 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgaa ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcatta
//
LOCUS       DAZSBF010000039        25568 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZSBF010000039 DAZSBF010000000
VERSION     DAZSBF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.039"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1809)
                     /gene="traD"
                     /locus_tag="ACHXWD_001231"
     CDS             complement(<1..1809)
                     /gene="traD"
                     /locus_tag="ACHXWD_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR7620199.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHQIAEFAAGEIGEKEHLKASLQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLF"
     gene            complement(1860..2597)
                     /locus_tag="ACHXWD_001232"
     CDS             complement(1860..2597)
                     /locus_tag="ACHXWD_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620200.1"
                     /translation="MTSDNIAEYIGENNQFTEKPATEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2800..3531)
                     /gene="traT"
                     /locus_tag="ACHXWD_001233"
     CDS             complement(2800..3531)
                     /gene="traT"
                     /locus_tag="ACHXWD_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFR7620201.1"
                     /translation="MKTKKMMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LKPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDAAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3545..4054)
                     /gene="traS"
                     /locus_tag="ACHXWD_001234"
     CDS             complement(3545..4054)
                     /gene="traS"
                     /locus_tag="ACHXWD_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFR7620202.1"
                     /translation="MITHYDIKTETQKLKDVLSVEGVNIPPLLQVIKPGVYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPKKFRDESKVISF
                     MYDKNKTYILSFVIVFSIVSFAHTFLFGFLSITLFVIISFIYTIDINRYNLSAIVSVI
                     GLFKKESVS"
     gene            complement(4051..6876)
                     /gene="traG"
                     /locus_tag="ACHXWD_001235"
     CDS             complement(4051..6876)
                     /gene="traG"
                     /locus_tag="ACHXWD_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFR7620203.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMSTRTWDSIEKIALTLSVVAV
                     AFMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVVSYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TINALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKMNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASTGSKFSYGGY
                     ADAHAEWGIKPGILGVGGGVGVRGGFKGSLDRTDDDSHSASSGSRATHDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKRAPQDAEAILTNTSSPEIAERRRAMAWFFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEERES
                     QKIIPEPSEEAVKKMVDDKKDRLKGPL"
     gene            complement(6873..8246)
                     /gene="traH"
                     /locus_tag="ACHXWD_001236"
     CDS             complement(6873..8246)
                     /gene="traH"
                     /locus_tag="ACHXWD_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFR7620204.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQVAQGLVGG
                     LFPQTQESQQKVCQDIGSESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNQ
                     NIIWEALKKNRMFDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDAREQGF
                     ISSTTIPVFKYLIDPQMLGVSNSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QAVMDNVLENLNQAQQQIAIFQSQVQVQQDALLVVERQMNYMRQQLSARMLSRYQNNY
                     HFGGNSL"
     gene            complement(8233..8625)
                     /gene="trbF"
                     /locus_tag="ACHXWD_001237"
     CDS             complement(8233..8625)
                     /gene="trbF"
                     /locus_tag="ACHXWD_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFR7620205.1"
                     /translation="MKEKINDTEPGIKQIEREIERGCDNAKKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(8606..8953)
                     /gene="trbJ"
                     /locus_tag="ACHXWD_001238"
     CDS             complement(8606..8953)
                     /gene="trbJ"
                     /locus_tag="ACHXWD_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFR7620206.1"
                     /translation="MPPVLWRGWIPFCRYTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMTCLHESS
                     EISEGTYDERKNK"
     gene            complement(8883..9428)
                     /gene="trbB"
                     /locus_tag="ACHXWD_001239"
     CDS             complement(8883..9428)
                     /gene="trbB"
                     /locus_tag="ACHXWD_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFR7620207.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQIYGEEKGAK"
     gene            complement(9415..9699)
                     /gene="traQ"
                     /locus_tag="ACHXWD_001240"
     CDS             complement(9415..9699)
                     /gene="traQ"
                     /locus_tag="ACHXWD_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFR7620208.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            9780..10115
                     /locus_tag="ACHXWD_001241"
     CDS             9780..10115
                     /locus_tag="ACHXWD_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFR7620209.1"
                     /translation="MKKLSFKEKLEIVRNIIRESLSGNAAFALLVYSLLHSLPIKTFA
                     DLFVTILLFIAIIAVVIWLFLIIYIYFCELFRSHWIAVWFILWSVVINLTIGFGIYDK
                     LAPVISLMK"
     gene            complement(10096..10437)
                     /gene="trbA"
                     /locus_tag="ACHXWD_001242"
     CDS             complement(10096..10437)
                     /gene="trbA"
                     /locus_tag="ACHXWD_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFR7620210.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSAIWSG
                     VMWFFIISLVK"
     gene            complement(10451..11194)
                     /gene="traF"
                     /locus_tag="ACHXWD_001243"
     CDS             complement(10451..11194)
                     /gene="traF"
                     /locus_tag="ACHXWD_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFR7620211.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(11187..11444)
                     /locus_tag="ACHXWD_001244"
     CDS             complement(11187..11444)
                     /locus_tag="ACHXWD_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFR7620212.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGIL
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11471..13375)
                     /gene="traN"
                     /locus_tag="ACHXWD_001245"
     CDS             complement(11471..13375)
                     /gene="traN"
                     /locus_tag="ACHXWD_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFR7620213.1"
                     /translation="MKEKINTFFRSLNFNYSVKPCNLCAIVFFLSFSVFADSNSDYRA
                     GSDFAHQIKGQGSSSIQGFKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWA
                     TGETGKTITESFMNKPKDILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEF
                     TNYTCERDTMVEEYCTRTASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPV
                     TGTVESASLSVYAAFFFLNSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLT
                     GSGCTANGNCLPHGNGDRKVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNK
                     ADGVLTGTECSEPGGTKTGVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPAC
                     TLASRQCAFYSDEGTCLHEYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKNSDF
                     GEAVSQLAALAAAGKDVAALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLA
                     KCNSEEKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNG
                     QLRIGFGSAKHPDCRGITVDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQ
                     IADQLKQAGQ"
     gene            complement(13611..13991)
                     /locus_tag="ACHXWD_001246"
     CDS             complement(13611..13991)
                     /locus_tag="ACHXWD_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620214.1"
                     /translation="MTLPAGYYQIDPEIRALVAAMNIHGFRTYASCQGHGFPVTKLPP
                     YIAFACPVKMAALLEQRLRQDAESAIPRLAWGWSVKGAFNSKFQLCFRLQPDTPHYWY
                     NRYCRHSLCADFRTLISLLKSLSE"
     gene            complement(13988..14626)
                     /gene="trbC"
                     /locus_tag="ACHXWD_001247"
     CDS             complement(13988..14626)
                     /gene="trbC"
                     /locus_tag="ACHXWD_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFR7620215.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVARALLNNPGDKQK"
     gene            complement(14635..14940)
                     /locus_tag="ACHXWD_001248"
     CDS             complement(14635..14940)
                     /locus_tag="ACHXWD_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620216.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(14970..15962)
                     /gene="traU"
                     /locus_tag="ACHXWD_001249"
     CDS             complement(14970..15962)
                     /gene="traU"
                     /locus_tag="ACHXWD_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFR7620217.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15959..16591)
                     /gene="traW"
                     /locus_tag="ACHXWD_001250"
     CDS             complement(15959..16591)
                     /gene="traW"
                     /locus_tag="ACHXWD_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFR7620218.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(16588..16974)
                     /gene="trbI"
                     /locus_tag="ACHXWD_001251"
     CDS             complement(16588..16974)
                     /gene="trbI"
                     /locus_tag="ACHXWD_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR7620219.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16971..19601)
                     /gene="traC"
                     /locus_tag="ACHXWD_001252"
     CDS             complement(16971..19601)
                     /gene="traC"
                     /locus_tag="ACHXWD_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFR7620220.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(19727..20074)
                     /locus_tag="ACHXWD_001253"
     CDS             complement(19727..20074)
                     /locus_tag="ACHXWD_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620221.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(20102..20320)
                     /locus_tag="ACHXWD_001254"
     CDS             complement(20102..20320)
                     /locus_tag="ACHXWD_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620222.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPFNSVMETLNGGGQ"
     gene            complement(20419..20892)
                     /locus_tag="ACHXWD_001255"
     CDS             complement(20419..20892)
                     /locus_tag="ACHXWD_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620223.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNRIDNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDAR
                     QELLNRLLSGKKLTGRDEKDIRRLWQEKVDAMQESATRQREQDTIRKFAEESKVC"
     gene            complement(20885..21298)
                     /locus_tag="ACHXWD_001256"
     CDS             complement(20885..21298)
                     /locus_tag="ACHXWD_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620224.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(21291..21512)
                     /gene="traR"
                     /locus_tag="ACHXWD_001257"
     CDS             complement(21291..21512)
                     /gene="traR"
                     /locus_tag="ACHXWD_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFR7620225.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(21647..22162)
                     /gene="traV"
                     /locus_tag="ACHXWD_001258"
     CDS             complement(21647..22162)
                     /gene="traV"
                     /locus_tag="ACHXWD_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFR7620226.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(22159..22479)
                     /locus_tag="ACHXWD_001259"
     CDS             complement(22159..22479)
                     /locus_tag="ACHXWD_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFR7620227.1"
                     /translation="MNMRNINVITARVVPDKNVSDDFMHAVLSNCTTKIVLPAPKVFS
                     SESLPHKFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKTNLTAELISAVVGSNKK
                     GEGQ"
     gene            complement(22466..23056)
                     /gene="traP"
                     /locus_tag="ACHXWD_001260"
     CDS             complement(22466..23056)
                     /gene="traP"
                     /locus_tag="ACHXWD_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFR7620228.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(23046..24473)
                     /gene="traB"
                     /locus_tag="ACHXWD_001261"
     CDS             complement(23046..24473)
                     /gene="traB"
                     /locus_tag="ACHXWD_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFR7620229.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGTSKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(24473..25201)
                     /gene="traK"
                     /locus_tag="ACHXWD_001262"
     CDS             complement(24473..25201)
                     /gene="traK"
                     /locus_tag="ACHXWD_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFR7620230.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(25188..>25568)
                     /gene="traE"
                     /locus_tag="ACHXWD_001263"
     CDS             complement(25188..>25568)
                     /gene="traE"
                     /locus_tag="ACHXWD_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFR7620231.1"
                     /translation="NSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRPGAQN
                     QMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHY
                     ILILKRENGVTWLDNFGETDDEKK"
BASE COUNT         6239 a         6935 c         5942 g         6452 t
ORIGIN      
        1 gaagaggctg gccatctggc gaccttctgc ttcccttgca gcaagtacgg cactcagacg
       61 gttctccatt tccgggttga tgtcacgcgg aataaactcc ggagcgacct tcggtcgtgt
      121 ctgatatttc agtgagagtt ttactgccgg atacggtccg ggcagagtga cataacaggt
      181 cagatccggc agagactgaa tgtcggaata actgaccagc gtctggcgct ccatctcctt
      241 accggtcgat accccgtcac gcacggggtc agcaccgtag gaatactgca gactggcttt
      301 caggtgctct ttctcaccaa tttcaccggc agcaaactct gcaatctgat ggctcgggga
      361 gcggaaaaag gcacgggtat tcagcacatc aaacagcgtg gccgccgctt tctcaccgta
      421 gatatcttcc agctgggcat aggactggat accaaacaca taacagcctc cgaacttacg
      481 ggcttccggc aggatctcca ccaggtccgg cagtttgtgt aacgtgggta actcatcaca
      541 gaaaaaccac acacgacggt tacggttttc tcccattgcc agcagaccac gaatggcaat
      601 ggacagccac atggaaacca ccggcttcag ggaggcatga gtgtcggcat ttgacgagat
      661 aaacagccag ccgtttttct gatcttcccg gacaccccgc atccagtcac gtatggtgaa
      721 gggttcaccg ttatgctcaa tcccctgcag gtaacggatg gctttcacgt agttggtcag
      781 cacagcacgg atggaaatcg ccgttttctc aattttctct tccaccaggt tggccgccgg
      841 tgaattacgc aggaaggtac gcagtttttc gattttgatg gaaagcagtg tgtccaccag
      901 tttgctgtag ctgcggttgg ggtcattacg catcagatac gccgcttccg caaaaatggt
      961 acgtcctgat ccctgccaga acgggtcctc tttcgttccc atcgggatca gcgtgttcgc
     1021 gacattatca aaatccggct gtgtcaggca ctccttccac aaatcccagg cggcacagcg
     1081 ggcatccagc ggattcagga tcttatcgat ggagggatca tagtaacttt taacaaactc
     1141 acacgaacgg tcataaatca ccaccatgtc gccacgttta cgggcatagt tggcaagacg
     1201 tcgaataacc tctgacttac ccgtcgacac tgttccgtgc agacagaagt tctggatttc
     1261 ggaatcccgg ataatcggca gatcgccaat ccggatatcg gagtccttgc cgtctttttt
     1321 cagcatccgg gcaacgtctt tcggattgtc tgtcagctga cgaccacctg tgacttcatt
     1381 ttcgctctgt tgtttaccct gacgccccag aatccaggag acaacaaaga aggtaatcag
     1441 gcaaataacc agtgccacaa ctgtagccag aacgaatgcg gaccatagct gctctccgca
     1501 ccagatcata tatttatcat gcagtacctg agcagcgttc atccggaagg ttttgccgta
     1561 atactggatc tcatagaccg gctgggattt tattaaatcc cgcatgcctt ccagtgacgt
     1621 acaccaccag taaatacagc cgttgataaa cgtctgccag cttattttta cccataagac
     1681 cagaccaatg aggatccaga aaaaaataaa caggcagtaa agcatgatat tggcgatctg
     1741 gctgaacatg cggatacgca tggacgcaat ctgaccgccc tgggtcatat cctttgcgtt
     1801 aaaactcatg atgaaactcc gggaaaatct ggaacagggc agaaaaggat gatgtggggt
     1861 taattcacag aaacagacca ggtgtaccct gtatgccggt ggtgatgccc gcctgcagtc
     1921 ctgccggaag agaccttctc tgtaacaatg tcagtcaggc gcaatacttc agaccagtca
     1981 ctcccatgcg gaaactggat ctgaataaaa aggcgtaccc gttcacggtg atccagaact
     2041 gtgtccggtt cataaaaacg gccactgtca ggagaaacac aggacgtttt attgatgaga
     2101 tcatacttat cggcaggaga ctgcgatgaa atcgcccagg tgatattctc cgcctggtgg
     2161 ccatcaatac gactgctccc cggcgtccat gcaatttcct gcgcactcgc cacgatgatc
     2221 tccctggaaa cttcagccag ctttctgggt gtacagaata ccggtgccag cacacacagg
     2281 gacaacaaat tacagctgac cgcctccggt ttaagcaaat tgctcccgga tttttcagga
     2341 aaagatattt ttgtcagtat ttcagttaat tccttatgcc tgagagaatt aataaagtac
     2401 atgataagca tcatgagaaa caatataaca ataagccctc cgataaccac taaaaggtca
     2461 gtatcaatat tattgctgta cagatagtct ttagtggtca ctgtattatc ctgtatcacc
     2521 ggggggtatt cagttgcagg cttctcagta aattgattat tctcaccaat atattcagcg
     2581 atattgtctg atgtcataat gactttctcc ggaagatccc ggcttcgccg gcaacacttc
     2641 acggtcgcac aaagaatttg cacaaccgct cagtgttttc atatttattt cggaaacgcc
     2701 gggaggtgac aaaatttatt ttgttacatc ccggggttgg acaacgagca acgcgaggcg
     2761 tcagcaagga ggccggtcag atcagcctcc tgacgaggtt cagagaatat ttgcgattga
     2821 tttggccagc tggtcttcga gaacaggctt cgcctcttca aatttcaggt taaccttgtt
     2881 cgcatttgag accacacggg tctggtattt atgctggtta cctgtttcag tactggtctg
     2941 aattttcgca ccggatgtgc cctgacgcag ggcggcaaca ttatccgttg tcaccgttgc
     3001 cttagtacgc tctgcaatct gcacatccgt gatcatggta tagttcacat cttccaccat
     3061 cgcatctgca gccataccca ccagaccagc agcaaggccc acaccgagtg tggcaccggc
     3121 agaatttgag ttatagccgg taataccggc acctaacgct gcaccaacag ctgcgccttc
     3181 ataaccacgg ttcagccatc cctgagactc ccgcagatcc atcttatcgg ccttcagcac
     3241 attcgcctga atccagtagt aggctgcatc cggagaagtc accacctgat atccttttgc
     3301 tttcacagca tcagcaattt tgccctgcag cccactcatg tctttatcag acgtgttttt
     3361 gatctgcaga aataccgtgc gttcgctggc gggtttaagc cagatggtct cactcatctg
     3421 agtcttcacc tcaaggttac gcttcttgat tgctgtgctc atcgcaccac accctgaaag
     3481 ggccagagtg gaactgacca gtgcaaccat catcattttt tttgttttca tccagaatat
     3541 ccttttaact aacagactct tttttgaaca gacctattac tgagactatt gcagagagat
     3601 tataacgatt tatatctata gtataaataa aggaaataat tacaaacaga gttattgata
     3661 aaaacccaaa taagaacgta tgagcaaagg aaactattga aaaaacaatc acaaacgaca
     3721 agatataagt tttgttctta tcatacataa aagatataac tttcgactca tctctgaatt
     3781 ttttagggat ggcaagatac aacatcattc cattagtaat ggcaacaaaa aggataaaac
     3841 ccattacgcc actaaaacat atatcaaatc cagcatctcg gatatcaact ttatctgcca
     3901 gcaaacgaag aaatgtcggc cataataaaa cccataaaaa cacatacacc cctggtttta
     3961 tgacttgtaa taatggcgga atattaactc cctctacaga gagtacatcc tttagtttct
     4021 gtgtctccgt ttttatatca tagtgagtga tcataatggc cccttcagtc tatccttttt
     4081 atcatcaacc atttttttca cagcctcttc tgatggctca ggtattatct tttgactttc
     4141 tcgctcttca ttatatttat tattctgaga taatgcctct gttttatgat gattttgcag
     4201 ttcagaatac tgccctctta caatattttc ctcgccacgg atactgttct gagtatctcc
     4261 gatattgcca cgatattctg tgaccatatt atcaacctga tgttttacat cattacggat
     4321 attactgtcc tgcgttcttt gctcaataat ggcctgatgt ccctgatggt cagcaataat
     4381 atcctgactg ccaccacccg aaggtacgct ctccattcct ttaccaatat cgccacggga
     4441 ttcccgccag gcattatcaa caccaggctg tacctgttcc tgcacaaaaa accaggccat
     4501 cgcccgacga cgttctgcaa tctccggcga actggtattt gtcagaatag cttctgcatc
     4561 ctgcggtgcg cgtttcatca catactgtgc aaactgttgc gacaggtctt cactcatctg
     4621 cccgctcatg ctttcagtac gggaggccat ttccgcatat tcatggctgc gggtcatatt
     4681 cgtcgtgtac tggtcgtaac tctgtttcgc tgagttcagg gctgcagaca actggtccac
     4741 ccgggagtca gcattattgt cagtatggct gccggactca ctgaccttgc gactggtaaa
     4801 gtaatcgctg gcttctttaa agtccttact ggctttagcg tcaatatcat ggcgagcatc
     4861 atgggtagct ctgctgccac tacttgcaga atgtgaatca tcatccgttc gatcaagact
     4921 tcccttaaac ccgcctctga caccaacccc accaccaaca ccaagaattc caggtttaat
     4981 cccccattca gcatgagcat ctgcatatcc gccatacgag aacttagaac ctgttgatgc
     5041 cagttcctga gtcgcctgct cattactgat attatgcgcc ttcgcatagc tttccactgc
     5101 actgcgcata cggctggcca tcatggagtc ctgtgcactc atggtgctgt cagcgccacc
     5161 ggtgacagaa tcactgctcc cccggttaga accaaactga ctgagcgtgt tccaggcact
     5221 ggcaatactg ctgctgaaac catgcagggc actttctgct ctgttcgacg cctcccgggc
     5281 catttcctgt tgcgccgctg caatctgacg cgttgcattg ataccgaccg gtaaacggga
     5341 catcgctccg cttgcatcca tcaccatatt accgtcacgg gtctgtgtgg cagttgcgcc
     5401 actgccggtc tggtacatca tctgaccaaa cgacgtggtg ctgttggtgc tccagctgaa
     5461 gccgttcacg ttttccgtct gcatgttgcc gtaggagtaa ttaccgtcaa ccacactgcc
     5521 tgctgcactg gccgtcggac tgatagagga agaggcgaag tggctgtaca cgctggaaaa
     5581 acctgcgcca aggcctttta ccatcatcca tgacaacggg gggatcatgg ctgaaagata
     5641 cccggcagta gaggccaggt cagagtattt cagctgtatc tgagagagtt cagacagcac
     5701 gaccggcgca ccattcatct tcgcatagaa tgtcatggca ctgttcagaa tggcatacaa
     5761 caacggccag gtctgcagcc acatcagggc aaacacatag cctttcagga cggacagtgt
     5821 cagtttgttg aagacagctg ccagtaccag aagcgggaaa ataccaatgg caattcccgt
     5881 caggatagtc tgtaccattg gcaggtttcg cattgtcaca tgaccgatag agacatgcgc
     5941 cagacgttgc ttctccatcg atgacgtggt ggccagattc accaggctgg cggtgtcacc
     6001 attacgggca gcattactgg tgataccctc tttcagggca tttattgtga cgttctggcg
     6061 cataatctgg ctggcagact gactggagcc gtagaaataa ctgtaactgt cactgaccag
     6121 ttgtctgaat aacaggtccg ggtccggtct gccgccaaat atctgctgca cataataatg
     6181 ccaggtcttg cctcccgtct ttgtatcgag attcagcctg tctttcagcg tgaccgacgc
     6241 atccttacag gttatgaaat tattattgtt gtcataaacg ccccgtagcg ggctgggtcg
     6301 ggaaaaaatc agcgtgtagg ggtcagcgga ggccatcaga tcttccagcg tgtatttatg
     6361 attcaggtaa atatcaccca gcacgcagtt ctggacgtag tcctggaaca gattgatgat
     6421 ttccggattc cgggacagga aatcggtgct tttcacaatc agattcgccc cgaacagcat
     6481 gcctgtcttg ctgtaggtga cgctgtccgg ttgggtgaat atcatctcgt agctgaccac
     6541 catcgcatgc ccgatacggg tcgtaagaga aagtggcatc gccagaccga ccggaacatt
     6601 atccacccga tggactttga ccaggtcact gttatcaata atctgcaccg atgtgcggac
     6661 attaaccagc aggctgataa gcacaaacac ggccacccag cccagcaaat ccatcacgtt
     6721 atgccgctgt acccacataa aagccacagc aacaacagac agggtgagcg cgattttttc
     6781 aattgaatcc caggtccggg tactcataaa tgcagcaatg gcgttcaggt tattccgcaa
     6841 ccactcacca ccggcaatca cataaacttc attcacagtg aattccctcc gaagtgatag
     6901 ttgttctggt aacggctgag catacgggcg gaaagctgct gacgcatgta gttcatctga
     6961 cgttcgacaa ccagcagcgc atcctgctgg acctgtacct gactctggaa aatggcaatc
     7021 tgttgctgtg cctgattcag attctccaga acattgtcca tcacagcctg cggatagttc
     7081 ccggtggcaa tcatggcccg ggcctgctga agcagttcct gaatgtactg gagcatgatg
     7141 tcgtagccga tatagtccgt cagctggtaa acgacgctgt tggacacacc gagcatctgc
     7201 gggtcaatca gatatttaaa aacgggaatg gtggtgctgg aaataaaccc ctgttcccgg
     7261 gcgtcaagcg gagtgtcact cactgcttta ttctgaatac tggtgagcag ttttgtaatc
     7321 tgtgacttca gggctttatc ccgggcgatg gtcacatttg catccgccac caccttcaga
     7381 cacttgtccg aatcgttgca gtggtaaatt tttgccgttc ccccctccat aatcgcccgg
     7441 aggatatcgc ggtcagtggt acgcgccggc aggggggtaa tctcgctgtc ttccccaaaa
     7501 atcagcgtac cggtgagcgt catgataaat tctttcaact ctttgttgcc gtcaaacatt
     7561 ctgttttttt tcagcgcctc ccatatgata ttctggtttt tcagcacccg ctccttgtcc
     7621 ttgtcgctgg ctttgtcacg aacagatgag gactgccctc cgaccgtgca cccctgacga
     7681 gatgcggccc agtcggcgaa gatattgctt tcactgccaa tgtcctgaca aactttctgc
     7741 tgagattcct gcgtctgagg gaacagcccc cccacaagcc cctgtgccac ctgacaggaa
     7801 ctgaggttca tactgttaat gtcacttgcc attttctgca ggaagtcttt tgcggttttt
     7861 atttccggca ccgttgtctg cagggcaagg tcaaaaaagt aaccggcagc attgctcata
     7921 atctgcttaa caaaacgctg caactgatcg ccattaataa aactgaacga gccgagatag
     7981 gcgtcgatgc cgccacatcc ggcattgata tccggcagcg tcatggaaat cagctggata
     8041 tttttgacct gggtgcgggc atacagggag ccaccatagg catacccggc ggcctgcccc
     8101 tgccagacgc ccggctgtgt ggtattggag gcgaagccca gcttgttaaa gaactgattc
     8161 atatcgctgt tcacatccgc tgacgctgcg ggtgtgaccg tcagtaaagc ggcacaaaga
     8221 accagaagag gtttaatgcg aagcattatt gctgccctcc cttaatgtgt tctcccggcg
     8281 catacggcgt ttcagcagga atatccggac acccccaaat attatttcgc acaacggcga
     8341 cagagaagcc gcagcaagaa aaccagctgc aagcgcatat aacataatcg gaatgacata
     8401 catcagggtg ttccacatat aacgaaaatt atttaattcc gggtcagctt tccacgaatc
     8461 aataccggca ctgaaaaaat catgcataac gttccttaca atcaatccgg cagcaaagaa
     8521 aaccacaaaa agccagaagt atttttttgc attatcacaa ccacgttcaa tctccctttc
     8581 aatttgtttg attcccggtt ctgtatcatt tattttttct ttcatcataa gttccctctg
     8641 atatttctga tgattcatgc agacaggtca tgcggctgta atatatttca gacaccgaaa
     8701 tccatcccac aattgccccc acaaaaaaca caacagaaac agaaaaggta agacacagcg
     8761 aaacgacaat ttccacaggg ctggcgggat aaaaaagccc ctgacggata aaggggatat
     8821 tcaggctgac gattattccc gtcgcgatgc cggatattcc ggcaatgagt aacaccacct
     8881 gcttatttcg cacctttttc ctctccgtat atctgcagaa cggtatccat ccgcgccata
     8941 aaactggcgg catccgttgc cccctgcaaa agcggtaatg cctcaagtgt gttgacgttg
     9001 accaggaagg tggtcggtgt ggccaccggg atattcggga agaaggtctg catcacgtcc
     9061 ggtggcaccg gtaatgcttc cggaaaggca gtatccccct gaccatccag ggtgtaggga
     9121 aaaacggaaa agccgtactg ctgtgccagc tgcttcagta ccgggtcaaa ctggtgacag
     9181 taagggcaat gcccctgcat aaacagcacc accttccagt cggccaggtt aacctgcctg
     9241 ccgttgctga ggcggaacca gcggggagcc ggcttttctg ttctggctga tgtgtctgct
     9301 gccggttgtg caggctgcac ggccattccc tgcggattcc agagtcgttc aatttcatcc
     9361 cgggtggagg cctgcacacc tgtggccatc atcaacaaaa tgaccggcag taatttagtg
     9421 agagacatgt ccgccctcct gctgcccttc catcatggcc tgttgcctgg cttccagtgc
     9481 ctcacgctct tccaggctga cacgtgcaat ccatgcatcc agcacctggt atgcgccgaa
     9541 cagcacaagg atggccgcaa tcaactccgc cagaaaaaaa gccatccagg gtttgctgta
     9601 aacaagacgg gcgacgatat gaaaccagac acccagggaa aatacccaca ttcccgtaat
     9661 atcaagccgg ggtaaagaga atctgcgttt acttatcatg ttaaactccg gttattattt
     9721 acatgagagt attcatgtct gtaatattcg cgtttgtgtg atattaatca gaggtacaaa
     9781 tgaaaaagtt atcttttaaa gaaaaactgg aaatagtgag aaacatcata agagagtcat
     9841 tatcaggtaa tgcggctttt gctttactgg tgtattcatt attgcactcc ctccccatta
     9901 aaacctttgc tgatttattt gtgacgatac tgttatttat agcgattatc gctgttgtga
     9961 tatggctatt tctaatcatc tatatttatt tttgtgagtt attcagaagt cactggatag
    10021 cagtctggtt tatcctctgg tctgtggtaa tcaacctcac tattggattt ggcatttatg
    10081 acaaattagc accagtcatt tcactaatga aataataaaa aaccacatca ctccagacca
    10141 tatggctgac aacataatac aaacaaacat ccttctgaaa ctgtcaaact tcgtgataac
    10201 cggtatggta ctgactgcca ttgaaacaac cggtacggca cactgattca taaccgcacc
    10261 acaaagaaac aagaaattat ctggcagcat acatacactt atgacattta ctacaaagaa
    10321 gacaaacgag ctcagcagat aaactccttt attatttggt cctgtaaaga aataaccaac
    10381 aacccccacg ataaacaggc tggcacctgt aaaaaaacac aaatcccctg tatgcatata
    10441 taacctttgg ttaaaaattc ggtttaaaat cttcagaaac gttcaggaac tgttttgcca
    10501 ggtcgtcctg tgaaataaag ccgtatgata acgggcgaac actgccctgt ttcgggtcaa
    10561 ccagcatcat ggccgggaaa tatttcacac cgaggcgctg cgcctgcccc tggtcagtcc
    10621 gggaatccgg caacagcgga ttaatcacgc catccacgga aacgggaata acactcagac
    10681 catacgtatc ccggaagcca ttaatgacct gcgccagttg cccgtcgatg gggtcctgcc
    10741 cccggtaaaa aaacatgatg ccgtagtgtt cagccagttt cgcaatggcc tgtcgctgct
    10801 gcgcctggtc tgccgccagc tggttccgta ccgtgccgtt gtaatggctg tactgcaggt
    10861 tatagtccag ttcgggatgt gccagcatgg cctttttagc actcatggtg aaaagcccgg
    10921 cctgctgagt ccagtaattc tgcagccgga aatatttaac aaaattatcc acgccgggat
    10981 acagaatggc ttcgtacagc gcccgctttg ttgccgtctg cagcgcggcc agtttctgca
    11041 taatatccgg ctcctgacgg ggggctgcag gtacaggttt attttctttt tccttcggat
    11101 ttattttctc gttataccac tgccagcctg catctttccc tgacgccgga aaaataaagc
    11161 agcagagtaa cagtggcagt aatgctttat tcatcttcat tcctttcatc attttttctt
    11221 tcatcatctt tctgcaggcc agtcagggca gaaaaacaaa aatacagaat aaacaggagc
    11281 acaccggagt aaaggataac gaaagcagca gcaagtatcc cgtctgccgt agtttctttt
    11341 accgtatccc acgaccagat aattaccgga gaaatgacaa tcgctgtaat cgaaagccgg
    11401 ataagaaaat caacaagacg attagaagtg aatataactt tcatcatttc ccccggttat
    11461 tttccatacc ttactgtcct gcctgtttca gctggtcagc aatctgctct ttcactttct
    11521 gcgtcagaac gccactgtcc gggattttct ggttattcat caggtcttcg tagaagttag
    11581 tgaaatccag tctgtcgaac tgaattttct gcagctcatc aaccgtaatc ccccgacagt
    11641 caggatgctt cgcactgcca aacccgatac gcaactgccc gttacgcccc tgctgctgga
    11701 ctatctgcgc cagcttcgaa tcaaactggc agtagctgcg ctttttctgc agacagacac
    11761 ccagcacttt ccgggagcag aactcgccca cactcaccgt cagtttgttg tcctttgcct
    11821 ttcccagcgc cttttcttca ctgttgcact tcgccagccc cacatcctga ccccagccgc
    11881 catccttaca gcagttactg aagccggctg cggccttacg gcagaatttc gcctcacccg
    11941 tgaaagccct gacatccaca ccattcaggg cggcaacatc cttacctgcg gcggcaagcg
    12001 cagccagctg tgatactgct tcaccaaaat cactgttttt tccgctctgt gccttatcgc
    12061 attcaccgtc aaggcagaag acatcaccac cacagaccat cacttttcct gatgttctgg
    12121 actcgcagga gtacgtggcg tactcatgca gacaggttcc ctcatcggaa taaaacgcgc
    12181 actgacgtga ggccagcgta caggccggat tatcgacata tttctgacag gtgccgttat
    12241 ctgcggactg agtgacatat ttatcccggt atgcccagca tgcctgtgtg atgttccagg
    12301 gtttaccttc catcactccg gttttcgtac cacccggctc tgaacactca gtgcctgtca
    12361 gcacgccatc cgctttatta aacggacaac tctccaccca ctccacccgg ggaacccatt
    12421 ctttatcacg gaccttcatc ctgagtttca gcgtaaaggt ggaagcacca ctgacaagcg
    12481 attcatagac tttcctgtca ccattcccgt gcgggaggca attaccgtta gcagtacagc
    12541 cactaccggt cagaacctgc ccctgtgtca cctgcaaacc tgtcgccccg gacagggggt
    12601 aagtatccga ctgcccgttt gccaggccga cattaaaggt tgtattcatg aaggtataac
    12661 gggagttcag gaagaaaaaa gcggcgtata cagacaatga tgcactttcc actgtaccgg
    12721 ttaccggagc cgtgacactg aacaccagtt tcagcccgtt catactgaac cggaactgtg
    12781 agtgcggtat cgtcacttca cgatattcat ccgtgtattt ccagtccccg gtgatactgg
    12841 cagtccgtgt gcagtactcc tccaccatcg tgtcccgctc acaggtatag tttgtgaatt
    12901 cactgcggtt tatctcctgc gcactgcact gctgtccggt atttcccaca atgctgtcag
    12961 ccctgttcac cacatccctg ccggtctgaa tgaacggtgc atccggtgac agaatgtctt
    13021 tcggcttgtt catgaaggac tctgtgatgg tttttccggt ttcaccggtc gcccattccg
    13081 tggtgccgtc atttttcagg ccgccatccc cgccggcagt cacgccaccg taatattttg
    13141 tctcgtccgg attcgcgtta tagccgggga tactctcctg tggcttaaag ccctgaatac
    13201 tgctgcttcc ctgccctttg atctggtgag caaaatcaga accggcccgg taatcactgt
    13261 tgctgtcagc aaacacagaa aagctcagaa aaaaaacaat tgcacaaaga ttgcacggct
    13321 ttacggaata attaaaattc agagatctga aaaaggtatt tatcttttct ttcataacct
    13381 gaaccatact ggagaatgaa gtgatgtcat ctcaacttca gaactgtgtt gattgtggta
    13441 aagcattaag tcgaacagcc acaacgtgcg ggaattgcag ctcaacggac ccttttggaa
    13501 aaaaacggcg cagcgatcgc ttttaccagt ggtttgtagg gctgtttatt acggctatta
    13561 ttattgctgg cggattatgg tacatggggc ttcttgacgt accgggatta ctactcagat
    13621 aacgacttca gtaacgatat cagtgttctg aaatcggcac ataacgagtg acgacaatac
    13681 cggttatacc agtaatgagg ggtgtcgggc tgaagcctga aacagagctg aaacttactg
    13741 ttaaaagccc ccttcaccga ccatccccat gccagacgtg gtattgctga ttcagcatcc
    13801 tgccgcagtc gctgctcaag aagggcagcc atttttaccg gacaggcaaa ggcaatataa
    13861 ggaggcagct ttgtaacagg gaaaccatgt ccctgacagg aagcatatgt gcggaagccg
    13921 tgaatgttca tcgcggccac cagggcacgg atctccggat caatctgata atacccggca
    13981 ggtaaagtca tttttgttta tctcccgggt tattcagcaa cgcccgagcc acctgccggc
    14041 agtcccccgt ggcggccact ttctccagcg cctggccgac ccgcaggtta cctcggataa
    14101 tgtcgtatcc ctgactgcag aacaccacca gtgccggtac actgcgaatg ccgtactgtg
    14161 aaaacagcgt cgggtcgatc tgaacaccat cagtcgcgcc gtctttcacc agggacagca
    14221 cggcttcagc ggtggtcttc aggtcattat tcaccatgcc ccgcagtgtg gcgggtatac
    14281 cgtagtgccg ggtttcaccc agcatccgtt tcagcccctc ttcgggaatg gaaaacgaca
    14341 caaaatacag ggcaccctgc cggggtttcc cgtcctgcga agcctgctgt ttacgaacca
    14401 gctcatccag aaaatggtta tctgaacgct gaagggggtt ttccagcacc tgtttctccg
    14461 cccaggcttt cagctgatgg tcaggtttct ctcgcagttg tctgcttaaa ttttcctgct
    14521 gcttcaggaa ctggcggttt tcaggtgtgt tcacattttc tgaagcatga acagcaagat
    14581 gcatccctgc tgtcagagca aaaagtgcaa atattttccg gttcatgata tttctcactt
    14641 tctgctattt acaggcatga actcatcaga aaaaagataa ttgagaaaaa cagccataaa
    14701 cgcacatgta aatgtcacct tcagatataa tctgacaagt gtcacttgtt ctgatatatc
    14761 tgccttcttc cattcatgaa taatttcagc gagaaaactg tcaggtgcaa aataaaataa
    14821 tataacgggc accattacaa gaccaaaagg aaataatttt aatgttctgc ttatcagcct
    14881 gagcagcaca gcacttattt tcctgaggtg ctgaatatat ttactgtcca ttgttgccat
    14941 tttcatttcc tcttattcat taaaatgatt tacaggaata cacagttacg tttacgccac
    15001 atcaggtagc cgaagttttt ctttgtgttg ggcggatttt ttcccgtctc ccagcgcatc
    15061 acactgcgcc cgaacggatg gcactgcccg ctgtccggat acatattcac catctgataa
    15121 cgccagcgtt ctttgggcag gattggggac ggatattcat tacagacggc gttatttttc
    15181 ccgatggttt ccataatcat gccctgacgg tgtagtttga acgccatgcg ttcactgacc
    15241 agcagggagg actgcaacgg actggactca ttactcaccc agccattgaa cgggtacata
    15301 cttccctgcg aaccggcaca ccagaacaga acatcgagag gcatattaaa ggcgctggca
    15361 atggcatctg ctgcacaggc cccctgtgct atcggattgg caaagatgac agcttccgga
    15421 ttgagaatgg tggtcaggct gctgtccgtc caggtggggt caatttcaga aagataagcg
    15481 atatccaggt caccaccttc cagacagccc agcgatgtga tgatgttcag ccagtacgtc
    15541 agcgggtatt tgtaccagtg aacgtgatag aacgccccgt tcacctgctt ctcatccttt
    15601 ttcgccgttc cctgtgccgt tttaccaaaa gccggcaggc tgaagcccag gttaaccatg
    15661 catcccggtg accgggtgac gtccgtcagc gccatcggct cccagtaacc aatggccagc
    15721 ccgatacgcc tgaacagcgg cggcggtgcc ggacaaatct gaatgggcat cgacgggttc
    15781 gccgtgtcgg ggaccttacc ctgactgact ttgacactgc ccagcgagag cgggaaaata
    15841 cagctccagc agatatccgt gatcgggttt acaaaacgcc cctcacaggc agaatccgca
    15901 gacgctgcag ggacatgacc ggcgagaagg aataacatca gcagccacag gcttcgcttc
    15961 attttctgcc ctcctctgcc ggaataaatt ccaccttcag gaagcgatcg ccgggaacgg
    16021 cgctcacccg tgccgggacc tgatcaatgc cgaggcgctg gcagagcaca ccgttctggt
    16081 caaagtaaac acggctgtcc agagacttct gcatctccgg gatactgccc tgcacgagga
    16141 taattttgct ctccagtgtg ggcggcgtct ggcgtttcat ccaggccacc tgcgccggat
    16201 catcgccatt gatgaaatac agcgtctggt taaaaggcac atactgcagc ggattcatca
    16261 cttcaccctg acgggcaaac acccgccctt cgttatcccg gatatccgca gccagtctga
    16321 cggacgggtc aaacaaccgg ctgccgtatt tctccgtgcg tccgataccg ggcacggcag
    16381 ggggccgcag actgttgcgg atcacccgct ccttaaaggc atccattttc cggcccatct
    16441 caccggactg ctccagtgcc gtcaggcgct gcatgatcac cgtcagcatg tccggctcct
    16501 ttaccggcca cagatcgccc caggtgccaa gatcggcggc ggccacactc tgcccccata
    16561 tcagcagggc aatcagcccc cggcatctca tggttccgct ctcattcgcc gggcaatatc
    16621 ctgctggatt tcccgggtga tatccggagc cccctgtacc acggcaggcg acaccagaat
    16681 gaccgcgtgg tgtttctgct gccacgcctg caggctggct tccagtgccg tgttaaaacg
    16741 cgctgaaagg gcttttgact gtgcttctga cagttgtttc tgactggcgc tgtcaaaaaa
    16801 tgcatccaca gtctgtttca tgttgaaggc ggcagtcacc ggtgcgttca gtctgacaat
    16861 gccgtaactg acggctgcgt tcagtaacac cattgccagg catcccggca ccgtccacca
    16921 ccagtggctg cgtcgggggg ctgtaacatc agtcgttttt tgcgttgtgg tcatgcaaca
    16981 ctcctgtatt tctcatgttc ttccagccag gcttccagcg aagccatctc cggccctgac
    17041 ttcttccacg ccagctgcca caccgcctca tgaatactca ggccctccct gcgtttctgc
    17101 tgcacaaact caaaatccgg gccgtcagag ctgtacatgg cgcggcttaa cgggtccaca
    17161 aacagacggt gccaggagga atggttttcc acctgcagca ggaaggaact gaaccactgg
    17221 tctttggccg caccaaactt gccaatcatg tcgcgctgca gtggctggaa ctgatccggg
    17281 aataactggt tatatttcgc gaactccttc gcactctgtt tgaggataat tttgtaggag
    17341 gagttacccc atgccgcacg ggccgcactg gaggccttgt cagagtcaaa gtcgacgatg
    17401 ttctgtgtga tggtgatata ggcaccggta tggcggcgac aggtgcggta acctttttga
    17461 ataaactcac cgaccttgcg atttttgaag tccagcagac gccagccttc atcaatgacg
    17521 ttcagtttct tgagagtacg cggcgtgcgg tacatcctgt tctcgatgta gataatcagg
    17581 gagaacatca ccgcaaccag cagtgacgga cggtcttcca gtccgcccag ctccagcacc
    17641 accattttcg catcatcccg cagggacggc tcatcggagt taaaatactg gccgtaagtg
    17701 ccgttggcag tgtactggtc aagcagcaca atcatttcgt ccagacggct gcggatggtc
    17761 ggtgacccgg catactgctc gctgtcactg gcgtttttca ggaaatccac cacgtcatca
    17821 atacgtgccc tgttctcttt ggccagccag gaggccctga ccgcctgcag cagcagacct
    17881 tcatgcactt catccaggtt accgttgggg ctggccatca ctgacaactg gtcacggaca
    17941 cgctccgctg actggtcaat atcggtgatg ttcgcaaacg ggttaaaacg cagggtttca
    18001 ccgtccagat atacgccccc catgttctca cacagggact tgtagccatc ccccatgtcg
    18061 aacaccacgg caaagccccc ggagtccagc acgctgcgga taagtggctg tatcagtccg
    18121 gttttaccgg ccccggaggt gccacagacc gccatgttgt agttggtgtt attcatcccc
    18181 ttaaagaaga tatcgataaa cgccagctgg ttacggtagg tgggcgccag cagacctgcc
    18241 ggtgtcaggg ggttatccgc cactaacggc atcaggttgg ccacattaaa gctctctgcg
    18301 cgctgaacca caccggcctc tttcagctgt ttaaacagcc ctttcccggc cataaagggc
    18361 aggcaggtca ggaaattgcg catgtggtta aagcgcggtg aaatcagctc aaaaccgttt
    18421 ttacggaagc tgttcaggat gtcctgctcc acttccagtg ccgtttcatt attgtccttg
    18481 cagaaggctg tgatgttgag gaagtaggac acgacagaag actggccgga gcccagccgc
    18541 tggcgcagtt ccccccactc cttcgcctct ttctccacgg acggaaacca tttggcatat
    18601 gaggtcttcg acttcttctc caggtccatg tacttcaggt tggcttcgct gtgggttttc
    18661 acctggtctt ccaccaccag agtcagcgtc aggatgaacg ggcaggaaat ggacatttcc
    18721 gggttcagca gattgctgta gttgtcggcc atgttccaca ggaaggcgat ttccgggtta
    18781 cgggccagat ggaaattcag gatgcgggcc gtgctgttcc tgccgttctc acgcaggccc
    18841 agcgtcaggt aatcagcccg gacattcagg tcaaaactgt cctccacaca ctgataattc
    18901 agatcagaat acgggtccag ctgacgtctt ttcgggtaca gagagtccgg attatggtta
    18961 atcatctccc cgacaatctc gatgaaagcc tgtgcatcca ctgtctgtgt ggtgattttt
    19021 gcgccgtgaa aagaggcacg gatgattttc accagatttt ccatttccag aatgtcagcc
    19081 cggctttttt tcttcgaggg agaacagtac gagataaaca cccggtaatg acgcagggtc
    19141 aggggcagat tcatcccctc cggcagcggg aactgtgtcg ctgccgcttt catataatag
    19201 gcacgggtaa tggcgttaaa ccgttcagcc tgttcacctg accaggagaa ctcacgcagc
    19261 ccgtattcaa tcctgtcacc aaccagctga ctggacatca gatggataca caacggaata
    19321 ccgcgcggca gtttggtgcg cagcatgtga tcgagtgcct ccacaatgga cttattcgcc
    19381 ccattgatgg gaatggcttc cagcataaag cccatcgtgg tgtcattcat gaacagaccg
    19441 gattcctggt tgtaatcacg gtacggcagc agacggctga actgcgggaa gctcatttcg
    19501 cccagaactt cattggcctt tgcggattcg tcaggcagtt tcagtgctgt gacgagggag
    19561 ttaaccgcct gagtgacggc ctcaagtggg ttattactca tatgagtatt ccttagtgcg
    19621 tttaaaaaag gaaaatatca gtctgtggcc gtggatatat cacgcagtgc cattcctgaa
    19681 accacagcac aaataagaga tggaggcttt taacaggaaa ccaggattaa cgttccttta
    19741 ctgaatgagc atcatttaat gcctgaattt gtttttcgcg aatcttattg cgctcatttt
    19801 ctcttacccg tttcacctct gccatttcgt ttctttttct gcattctgat gcgattttat
    19861 ttgccttacc aagtgttaaa ggttcagact gataattaag tgtctcctga aatgcttctg
    19921 ctgcacaggg agtttcctgt aaaatctctg tcagttgttc tggtgttgca ggttgttgcg
    19981 caaggtaaac gatgtaaaaa ggaatacata caatcatagc cataacgcca acaaatataa
    20041 aagccagaac agattttaaa tatatcgact tcatttcatt ttccttcgaa gcagtaagca
    20101 ctcattgccc accaccattt agtgtttcca tcacgctgtt gaaaggttca tttgttttaa
    20161 cttcaataca ctcccctgta tttccaaagc ccctgccgcc atgtgcttcg attgtccgaa
    20221 gaacctgcac gcatacgtcc gtatttggca tgggtgtaat attccagctt ccgccaccgt
    20281 taatattttt tatcagcaca agaaaaataa aggtatgcat tctttattat ctccgcttgt
    20341 atattaatca ctgaatatcc cttcagttaa cttatgaggt cataaaacac tgcagatttt
    20401 cattcagatt tactctcttc agcaaacttt actctcttca gcaaacttcc ggatggtatc
    20461 ctgttcacgc tgacgggtgg cagactcctg cattgcatcc actttctcct gccagagacg
    20521 ccggatatcc ttttcatccc gaccagtcag ttttttaccg gacagcagac ggttaagcag
    20581 ttcctgtctc gcatccggta cgtcttcagt cagacgcata agctgaagca ggctctgacg
    20641 gctgagaacc atgagtgatt ccggcaggag ggtgaagaag aaaataaaaa agcataccgc
    20701 accacagcag gcaaggaccg caaacaggat tttcagatat ataccatcaa cgccgaacgc
    20761 agcaataata cagggaatcc atgcaataca cgcacaatac gccaggatgt tatctattcg
    20821 gttatgtctg cgatatatct gttgatgttc atccagaata gaatctattt tttcagcttc
    20881 tgatttatgc atagtgtttc ctctgcgttt cctgccagag acgccggata tcctgttcat
    20941 cccgaccagt cagtttttta ccggacaaca aacggttaag cagttcctgt ctcacatctg
    21001 gtacgtcttc agtcagacgc ataagccgga gtaattcctg acgactgagt accatgcgtg
    21061 attcctccgg tacgctgtaa taattaaaaa tacgactgaa taccccacaa cagcaggcaa
    21121 cggacaggca cagggatttc agatacaggc cagaaacaca gaatgtggca acaccacaaa
    21181 gaatagctat aactaaaaat cccttaccca gtgccataca caaagaaccc tgttttctgg
    21241 atatccgctc atgctcatcc agaataaaat ctattttttc attttctgat ttatgcatag
    21301 tgtttcctct gcctttcctg atacgcctga cattcaacgc acaacgtcac accgggaaat
    21361 attttccgcc gggcttccgg aataggattt ccgcatgctt cacagagata aacaggaata
    21421 ccatgaacat ttatttcctg gcgtatccgg tttattcctg tcatggtcag ttgttctgtc
    21481 actgaatatg cttcatcggc ttcatcactc acggaatatt cctcccgaca tgttctgaca
    21541 cgctaccgcc catccctttt cacgcacagg gcactcgttt tacaggcgga ttttgaatac
    21601 agaaagcccg ccggcattgc cggctgatat acaacttcag ggccagttaa ttaatacgtg
    21661 gttttcccca cgcagacggt ttaataacaa aaaacacgct ggatggctga tgataaacat
    21721 cctggttatc aatataaggc gctatccata atgctgccgt ctgctcaccc gttcttagcg
    21781 gacgaggagg tgttaccggc gtaactgaac tgaccggaac aaccgttttc acttcccggg
    21841 cagcggtaaa cagcgggcgt ggtgccagca gtttctgctc cggatgtgct gtcaccgcag
    21901 gtctgctgcc ggacggagtg gtcgcggtga cggtctgcac cggcattgtc cggaagttcc
    21961 cttcagccag gcgcggcagt gatgccgcaa ccggctttgc ttctgaggac tgctccagtt
    22021 ttttggcctt ctcattggcc tgctccatcg tcatacaggt atcggatgtg gtggcgttac
    22081 actcaaattc cgtatttgtt cctgcgcagc cggaaagtaa taaggttccc agcagaggaa
    22141 taaataaaga aatctgtttc attgcccttc tcctttttta ttactcccca caacagcaga
    22201 aattaactct gctgtcagat ttgttttccc tgcacaggga tgtccggaga ccactgtatt
    22261 tcccggagaa gaaaataagc tcatcgacat tcccttcttc atcacaccga cagcagccat
    22321 attaaatttg tgcggcaatg attcagaact gaacaccttc ggagcaggaa gaactatttt
    22381 tgtggtgcag ttactgagaa cagcatgcat aaaatcatct gatacgtttt tatccggcac
    22441 aacgcgggct gtaataacat taatattacg catgttcatt tttatctccg ttgcttctgt
    22501 ttgttcggtc atcagggaaa taaacatggc atttcctgat ttttcccggc agggaagcgt
    22561 cttccgtata aacgtccttc agatattcat ctgcggttac cacccggata catttcattt
    22621 ttccgggcag caccggatat ttcccggtga tcgctgccag cgagacatac atcagtgcca
    22681 gtgttgccca taatgcggtg accagttgca tcagtgaccg gtcagtctcc ctgacataat
    22741 ctgcagcatc agccgcgcgg taatggcaga tatcctgtat ttctgatctg cctgaagaat
    22801 cagaggaaga aaaccagcat tcactgaccg ggaactgtcc ggcaggtgct gtttgtctta
    22861 cgaagttaat ctccttaacc agtaatttcc ccggcgtggt gttgtccttc cataaaacaa
    22921 acagagccat cagggccatt gtggccagtg gcagaatgac ggtgtacttc agacaccaga
    22981 acagtcctcg cagaacccgg gcgacaacat accgggcggc atggcacgcc tgacgggacg
    23041 acatattatt tgccatcgtt gcctcctccc tggctgccgg acataaactg ctgcggattc
    23101 aggttgccca gctgcttcag catctggtca gtattaaagc cgttaagatg actttcagct
    23161 gatggcggaa caggaaccgg actttccgga ttatcttctt ccgctctgtt ctgcgtgcgc
    23221 tccagcgcca tctcttccac ggttttcagc tggaagccgt cctggaacac cacggtcact
    23281 tcgttgcccg caccaatcgg tatcaccggg tgatactgtt cggcacgttt gatgtagtag
    23341 tcactgagcg tctgtgcggc tttcgatgtg ccgccaccga tacccatttt caggacatca
    23401 ccagccccgt aagcggctgt ggcacccagc ccgacagccg gctgggaggc gcgctccatt
    23461 ccctgaccga taccgtcaac aaatcccgcg ccccatgccc agccgaggat tttgccgtta
    23521 cgcatcacca cttcgccctt gataccgttt ttaccccgga agctgacatg ccccttaatc
    23581 ggcatatcaa tagttttgcc gtccttcagg caactgatat tgcgggtgcg tacaatggca
    23641 cgctcactgg acacatcccc ccaggcttcc agccccacaa aacatcccgt tgcgtcatac
    23701 gtcttgctgt tcggcatttc caccaggccg gtgatacgca actgcatcgg caccgtggat
    23761 tcattaccgg tgactgaggc attggcatcc gccccttcaa tcagcatggc tttcgcaaaa
    23821 cttcctgacg gaatgtacgg cagcgatggt ccctgttttc cctcattacg ggtaaacacc
    23881 ttacgctgta tccggttagg caccggcaca gactggtacg tcacctgtgg tggtggcgtg
    23941 acaccattcc cgggataaaa cgccgtcggc ggtggaacag caacgctgcc ctgcggcggg
    24001 aaggatacgg gggtgtttcc tggctgtggt tcgccttccg gcccgggcgg tgaggcaggc
    24061 atctgaggta caggctcacc ggtcgccgtg acgggattag cacccagggc ttttacctgc
    24121 tctgccaggg cggcattgtc ctgtcccagc ttttcaatac gacgctggtc atcaccgcgc
    24181 tgtttgttca ggacatccag ttcacgacgg atttcctcat actgcttctg catctgcgct
    24241 gccgtcacct gcatctctgt ggtggcatgc tgacgcacct tgtcatcaaa ggtcgtatcc
    24301 accacacctg tcatatccgg cacaggctcc tgttcagcca ccgcctcacc gttaccggac
    24361 atgtccacat cagacagata cagtacccca ccaatcgcgg aggctgcacc gacaaccaca
    24421 atccccagcc acaggtactg cttgcgtttc acaatggtat tgatactggc catcagttgc
    24481 cctccccgtc acggatgaca tacacatcca tgcgcgcacc tgccagtaac tggctggcag
    24541 gctggctgaa catcacggca cgggttcccg gctgccagaa gtcactttcc cggatattca
    24601 gggcagacag cgttttattt tccacggcaa agcggaccat cttcaggtga ttccccgtcc
    24661 atacggcatc tgccactgaa gacagccccg ccggggcctg cagggtttcc tttgtcacag
    24721 gaacctgata ccagcctgca ggtaattttc cgccacggac agcctggctg atggtcacaa
    24781 gcagggattc gtaaggcgtg gacgtttccc acgctccggc ttcttctccg gttccgcgca
    24841 ggtcactgac cagctgaatg gtacgccccg cgccttcacg gggaacagcc tgaatggaaa
    24901 ggttcagacc acgctctgtt tccagaatga acgtaaaggg ctttttgttg acggtggcca
    24961 ccaccacacc gccggaggcg gtctgctcat tattggtcag ggcaccatcc agactgttca
    25021 cggcgataat ccggtcaccg ggaacggtaa acagattcgg gctggtattg ctgatggcaa
    25081 tactggcctg accaccgttc accattggca ccacagtggg ggccagcgta ccgtttgcgg
    25141 ccatcacgct gcaggaaaaa aacaggccgc cgaatattat tgccgtatta ttttttctca
    25201 tcgtctgttt ccccgaaatt atccagccag gtcaccccgt tttcccgctt cagaataagg
    25261 atgtaatgtt tgatatcagt aaaaggttcg gagttaccaa tccatgtttt ccgaattcca
    25321 cggatttcca cacgaccata ctgaggccag acacggacac tggtctggaa aaaagcagaa
    25381 ttcacgttat cttttttaat gagtttcgct tcttcagcca gaataacttt catctggttc
    25441 tgtgcacccg ggcggatata ttgcagaagc gcctgatgtg aagcatcgac gatttccggt
    25501 gagacattca gacggagggc aataaatgac agcgccatct gctgcaaata agaggcatcg
    25561 gcactgtt
//
LOCUS       DAZSBF010000040        25494 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZSBF010000040 DAZSBF010000000
VERSION     DAZSBF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.040"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..485
                     /gene="gspA"
                     /locus_tag="ACHXWD_001264"
     CDS             <1..485
                     /gene="gspA"
                     /locus_tag="ACHXWD_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR7620232.1"
                     /translation="SLQTLVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTW
                     TLTHKWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAE
                     WRPLLVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK
                     "
     gene            487..906
                     /gene="gspB"
                     /locus_tag="ACHXWD_001265"
     CDS             487..906
                     /gene="gspB"
                     /locus_tag="ACHXWD_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFR7620233.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHXWD_001266"
     CDS             1144..1455
                     /gene="rpsJ"
                     /locus_tag="ACHXWD_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR7620234.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1488..2117
                     /gene="rplC"
                     /locus_tag="ACHXWD_001267"
     CDS             1488..2117
                     /gene="rplC"
                     /locus_tag="ACHXWD_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR7620235.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2128..2733
                     /gene="rplD"
                     /locus_tag="ACHXWD_001268"
     CDS             2128..2733
                     /gene="rplD"
                     /locus_tag="ACHXWD_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR7620236.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2730..3032
                     /gene="rplW"
                     /locus_tag="ACHXWD_001269"
     CDS             2730..3032
                     /gene="rplW"
                     /locus_tag="ACHXWD_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR7620237.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3050..3871
                     /gene="rplB"
                     /locus_tag="ACHXWD_001270"
     CDS             3050..3871
                     /gene="rplB"
                     /locus_tag="ACHXWD_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR7620238.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHXWD_001271"
     CDS             3888..4166
                     /gene="rpsS"
                     /locus_tag="ACHXWD_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR7620239.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4181..4513
                     /gene="rplV"
                     /locus_tag="ACHXWD_001272"
     CDS             4181..4513
                     /gene="rplV"
                     /locus_tag="ACHXWD_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR7620240.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHXWD_001273"
     CDS             4531..5232
                     /gene="rpsC"
                     /locus_tag="ACHXWD_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR7620241.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5245..5655
                     /gene="rplP"
                     /locus_tag="ACHXWD_001274"
     CDS             5245..5655
                     /gene="rplP"
                     /locus_tag="ACHXWD_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR7620242.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHXWD_001275"
     CDS             5655..5846
                     /gene="rpmC"
                     /locus_tag="ACHXWD_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR7620243.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHXWD_001276"
     CDS             5846..6100
                     /gene="rpsQ"
                     /locus_tag="ACHXWD_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR7620244.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6265..6636
                     /gene="rplN"
                     /locus_tag="ACHXWD_001277"
     CDS             6265..6636
                     /gene="rplN"
                     /locus_tag="ACHXWD_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR7620245.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6647..6961
                     /gene="rplX"
                     /locus_tag="ACHXWD_001278"
     CDS             6647..6961
                     /gene="rplX"
                     /locus_tag="ACHXWD_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR7620246.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6976..7515
                     /gene="rplE"
                     /locus_tag="ACHXWD_001279"
     CDS             6976..7515
                     /gene="rplE"
                     /locus_tag="ACHXWD_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR7620247.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHXWD_001280"
     CDS             7530..7835
                     /gene="rpsN"
                     /locus_tag="ACHXWD_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR7620248.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHXWD_001281"
     CDS             7869..8261
                     /gene="rpsH"
                     /locus_tag="ACHXWD_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR7620249.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8274..8807
                     /gene="rplF"
                     /locus_tag="ACHXWD_001282"
     CDS             8274..8807
                     /gene="rplF"
                     /locus_tag="ACHXWD_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR7620250.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8817..9170
                     /gene="rplR"
                     /locus_tag="ACHXWD_001283"
     CDS             8817..9170
                     /gene="rplR"
                     /locus_tag="ACHXWD_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR7620251.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHXWD_001284"
     CDS             9185..9688
                     /gene="rpsE"
                     /locus_tag="ACHXWD_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR7620252.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHXWD_001285"
     CDS             9692..9871
                     /gene="rpmD"
                     /locus_tag="ACHXWD_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR7620253.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9875..10309
                     /gene="rplO"
                     /locus_tag="ACHXWD_001286"
     CDS             9875..10309
                     /gene="rplO"
                     /locus_tag="ACHXWD_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR7620254.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10317..11648
                     /gene="secY"
                     /locus_tag="ACHXWD_001287"
     CDS             10317..11648
                     /gene="secY"
                     /locus_tag="ACHXWD_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR7620255.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHXWD_001288"
     CDS             11680..11796
                     /gene="rpmJ"
                     /locus_tag="ACHXWD_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR7620256.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHXWD_001289"
     CDS             11943..12299
                     /gene="rpsM"
                     /locus_tag="ACHXWD_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR7620257.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHXWD_001290"
     CDS             12316..12705
                     /gene="rpsK"
                     /locus_tag="ACHXWD_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR7620258.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHXWD_001291"
     CDS             12739..13359
                     /gene="rpsD"
                     /locus_tag="ACHXWD_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR7620259.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHXWD_001292"
     CDS             13385..14374
                     /gene="rpoA"
                     /locus_tag="ACHXWD_001292"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR7620260.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHXWD_001293"
     CDS             14415..14798
                     /gene="rplQ"
                     /locus_tag="ACHXWD_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR7620261.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14905..15273
                     /locus_tag="ACHXWD_001294"
     CDS             14905..15273
                     /locus_tag="ACHXWD_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR7620262.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15284..15709
                     /gene="zntR"
                     /locus_tag="ACHXWD_001295"
     CDS             15284..15709
                     /gene="zntR"
                     /locus_tag="ACHXWD_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR7620263.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15765..15983
                     /gene="arfA"
                     /locus_tag="ACHXWD_001296"
     CDS             15765..15983
                     /gene="arfA"
                     /locus_tag="ACHXWD_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR7620264.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHXWD_001297"
     CDS             complement(15980..16390)
                     /gene="mscL"
                     /locus_tag="ACHXWD_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR7620265.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHXWD_001298"
     CDS             complement(16520..17896)
                     /gene="trkA"
                     /locus_tag="ACHXWD_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR7620266.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHXWD_001299"
     CDS             complement(17918..19207)
                     /gene="rsmB"
                     /locus_tag="ACHXWD_001299"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR7620267.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHXWD_001300"
     CDS             complement(19253..20200)
                     /gene="fmt"
                     /locus_tag="ACHXWD_001300"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR7620268.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHXWD_001301"
     CDS             complement(20215..20724)
                     /gene="def"
                     /locus_tag="ACHXWD_001301"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR7620269.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20854..21978
                     /gene="dprA"
                     /locus_tag="ACHXWD_001302"
     CDS             20854..21978
                     /gene="dprA"
                     /locus_tag="ACHXWD_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR7620270.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21950..22423
                     /gene="smg"
                     /locus_tag="ACHXWD_001303"
     CDS             21950..22423
                     /gene="smg"
                     /locus_tag="ACHXWD_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR7620271.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22452..22994
                     /locus_tag="ACHXWD_001304"
     CDS             22452..22994
                     /locus_tag="ACHXWD_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR7620272.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHXWD_001305"
     CDS             22999..23571
                     /gene="tsaC"
                     /locus_tag="ACHXWD_001305"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR7620273.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23576..24394
                     /gene="aroE"
                     /locus_tag="ACHXWD_001306"
     CDS             23576..24394
                     /gene="aroE"
                     /locus_tag="ACHXWD_001306"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR7620274.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24391..24648
                     /locus_tag="ACHXWD_001307"
     CDS             24391..24648
                     /locus_tag="ACHXWD_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR7620275.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24624..25178)
                     /locus_tag="ACHXWD_001308"
     CDS             complement(24624..25178)
                     /locus_tag="ACHXWD_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR7620276.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6492 a         6023 c         6679 g         6300 t
ORIGIN      
        1 cttctttaca aacattggta gatcagaatt tgccctggag cgcatcgctt aaagtcgggg
       61 acaaaaaatt gcctgttgtg gtcgttcgcg tcggtgaggc tagcgttgat gtcctcgtag
      121 gtcagcaaac gtggacgctg acacataaat ggtttgagtc ggtatggacg ggtgattatc
      181 ttctgttgtg gaaaatgtcg ccggaagggg agagtaccat tacgcgtgat agcagcgagg
      241 aagagattct ctggctggaa acgatgttaa accgtgcatt gcatatttcg acagaacctt
      301 cggcggagtg gcgtcccttg ttggtagaaa aaataaagca atttcaaaaa agccaccact
      361 tgaaaactga cggtgttgtt ggcttttcaa cgttagtaca tctctggcag gtggcagggg
      421 agagtgccta cttatatcgg gatgaagcaa atatttcccc ggaaacaaca gtgaagggga
      481 aatgacatgt ttgaattcta tatcgccgcg cgtgagcaga aagaaacagg tcaccctggt
      541 atcttctccc gacaaaaaca cagcaccata atatatgtca tttgtttatt gctgatttgc
      601 ctgtggtttg caggaatggt gttggttggt gggtatgcca ggcagctatg ggcgctttgg
      661 atagtaaaag cagaagtcac tgtagaggct gaaacgcccg cttttaaaca atcaacacaa
      721 cactatttct tcaaaaaaca gccactcccc gtcgtcgaat ccgtcgagga ggaagatgac
      781 ccagacgtcg cggttgagaa tgctccctct tcttctgaag acgaagaaaa tactgtaaaa
      841 gaaagcgacg agaaagccgg tttaagagag cgtgtcaaaa atgcgctgaa cgaacttgag
      901 agataacccg aaggctgttt actttactag caatacgctt gcgttcggtg gttaagtatg
      961 tataatgcgc gggcttgtcg tagttgacag caggttcaat ctgaacctca gttagccgaa
     1021 tttggctacc taacaatgct cccaatcggg gagctacgta agaacggtta cactctccca
     1081 tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat aaaataattg gagctctggt
     1141 ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt ttgatcatcg tctgatcgat
     1201 caagcaaccg cggaaatcgt cgagactgcc aagcgcactg gtgcgcaggt ccgtggtccg
     1261 atcccgctgc cgacacgcaa agagcgcttc actgttctga tctccccgca cgtcaacaaa
     1321 gacgcgcgcg atcagtacga aatccgtact cacttgcgtc tggttgacat cgttgagcca
     1381 accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg ctgccggtgt agacgtgcag
     1441 atcagcctgg gttaatcagg tcattgagcg attgagaggt tgaaacaatg attggtttag
     1501 tcggtaaaaa agtgggtatg acccgtatct tcacagaaga cggcgtttct atcccagtaa
     1561 ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa agacctggct aacgatggct
     1621 accgtgctat tcaggtgacc accggtgcta aaaaagctaa ccgtgtgacc aagcctgaag
     1681 ctggccactt cgctaaagct ggcgtagaag ctggccgtgg tctgtgggaa ttccgcctgg
     1741 ctgaaggcga agagttcact gtaggtcaga gcattagcgt tgaactgttt gctgaggtta
     1801 aaaaagttga cgtaactggc acctctaaag gtaaaggttt cgcaggtacc gttaagcgct
     1861 ggaacttccg tacccaggac gctactcacg gtaactcctt gtctcaccgc gttccgggtt
     1921 ctatcggtca gaaccagact ccgggcaaag tgttcaaagg caagaaaatg gcaggtcaga
     1981 tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt acgcgttgac gctgagcgca
     2041 acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg tagcgacctg atcgttaaac
     2101 cagctgtgaa ggcgtaagga gatagcaatg gaattagtat tgaaagacgc gcagagcgcg
     2161 ctgactgttt ccgaaactac cttcggtcgt gatttcaacg aagcgctggt tcaccaggtt
     2221 gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg ctcagaagac tcgtgctgaa
     2281 gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca ccggccgtgc gcgttctggt
     2341 tctatcaaga gcccgatctg gcgttctggt ggcgtgacct ttgctgctcg tccgcaggac
     2401 cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc tgaaaagcat cctgtccgaa
     2461 ctggtacgtc aggatcgtct gatcgttgtc gagaagttct ctgtagaagc gccgaaaact
     2521 aagctgctgg cacagaaact gaaagacatg gctctggaag atgtgctgat catcaccggt
     2581 gagctggacg aaaacctgtt cctggctgcg cgcaacctgc acaaggttga cgtacgcgat
     2641 gcaactggta tcgacccggt tagcctgatc gccttcgaca aagtcgtaat gactgctgat
     2701 gctgttaagc aagttgagga gatgctggca tgattcgtga agaacgtctg ctgaaggtgc
     2761 tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat ggaaaaatcc aacaccatcg
     2821 tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa agctgctgtg cagaaactgt
     2881 ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa agggaaagtt aaacgtcacg
     2941 gacagcgtat cggtcgtcgt agcgactgga aaaaagctta cgtcaccctg aaagaaggcc
     3001 agaatctgga cttcgttggc ggcgctgagt aagtcggagg agtaatacaa tggcagttgt
     3061 taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt aaagtggtta accctgagct
     3121 gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac agcaaatccg gtggtcgtaa
     3181 caacaatggc cgtatcacca ctcgtcatat cggtggtggc cacaagcagg cttaccgtat
     3241 tgttgacttc aaacgcaaca aagacggtat cccggcagtt gttgaacgtc ttgagtacga
     3301 tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa gacggtgaac gccgttacat
     3361 cctggcccct aaaggcctga aagctggcga ccagattcag tctggcgttg atgctgcaat
     3421 caaaccaggt aacaccctgc cgatgcgcaa catcccggtt ggttctactg ttcataacgt
     3481 agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc gctggtactt acgttcagat
     3541 cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt tctggtgaaa tgcgtaaagt
     3601 agaagcagac tgccgtgcaa ctctgggcga agttggcaat gctgagcata tgctgcgcgt
     3661 tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt ccgaccgttc gcggtaccgc
     3721 gatgaacccg gtagaccacc cacatggtgg tggtgaaggt cgtaactttg gtaagcaccc
     3781 ggtaactccg tggggcgttc agaccaaagg taagaagacc cgcagcaaca agcgtactga
     3841 taaattcatc gtacgtcgcc gtagcaaata attttagagg ataagccatg ccacgttctc
     3901 tcaagaaagg tccttttatt gacctgcact tgctgaagaa ggtagagaaa gcggtggaaa
     3961 gcggagacaa gaagcccctg cgcacttggt cccgtcgttc aacgatcttt cctaacatga
     4021 tcggtttgac catcgctgtc cataatggtc gtcagcacgt tccggtattt gtgaccgacg
     4081 aaatggtcgg tcacaaactg ggtgaattcg caccgactcg tacttatcgc ggccacgctg
     4141 ctgataaaaa agcgaagaag aaataaggta ggaggaagag atggaaacta tcgctaaaca
     4201 tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt gctgacctga ttcgcggtaa
     4261 gaaagtgtcg caggctctgg atattttgac ctacaccaac aagaaagcgg ctgtactggt
     4321 caagaaagtt ctggaatctg ccattgctaa cgctgaacac aacgatggcg ctgacattga
     4381 cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg agcatgaagc gcattatgcc
     4441 gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc agccacatca ctgtggttgt
     4501 gtccgatcgc tgagactctg gagactagca atgggtcaga aagtacatcc taatggtatt
     4561 cgcttgggta ttgtaaaacc atggaactct acctggtttg cgaacaccaa agaattcgct
     4621 gacaacctgg acagcgattt taaagtacgt cagtacctga ctaaggaact ggctaaagcg
     4681 tctgtatctc gtatcgttat cgagcgtccg gctaagagca tccgtgtaac cattcacact
     4741 gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg tagaaaaact gcgtaaggtc
     4801 gtagcggaca tcgctggcgt tcctgcacag atcaacatcg ccgaagttcg taagcctgaa
     4861 ctggacgcaa aactggttgc tgacagcatc acttctcagc tggaacgtcg cgttatgttc
     4921 cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc tgggcgctaa aggtattaaa
     4981 gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac gtaccgaatg gtaccgcgaa
     5041 ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact acaacacctc tgaagcgcac
     5101 accacttacg gtgtaatcgg cgttaaagtg tggatcttca aaggcgagat cctgggtggt
     5161 atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc ctaaaaagca gcagcgtaaa
     5221 ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc gtacaaaatt ccgtaaaatg
     5281 cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg ttagcttcgg cagcttcggt
     5341 ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga tcgaagcagc acgtcgtgct
     5401 atgacccgtg cagttaagcg tcaaggtaag atctggatcc gtgtgttccc ggacaaaccg
     5461 atcactgaaa agccgctggc agtgcgtatg ggtaaaggta aaggtaacgt ggagtattgg
     5521 gttgccttga ttcagccggg taaagtcctg tatgaaatgg acggtgttcc ggaagagctg
     5581 gcccgtgaag cattcaagct ggcagcagcg aaactgccga ttaaaaccac ctttgtaact
     5641 aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa gagcgttgaa gagctgaaca
     5701 ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg tatgcaggct gcaagtggcc
     5761 agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga tgtcgcacgc gttaagactt
     5821 tactgaacga gaaggcgggt gcgtaatgac cgataaaatc cgtactctgc aaggtcgcgt
     5881 tgttagcgac aaaatggaga aatccattgt tgttgctatc gaacgttttg tgaaacaccc
     5941 gatctacggt aaattcatca agcgtacgac caaactgcac gtacatgacg agaacaacga
     6001 atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt ccgctgtcca agactaaatc
     6061 ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa tacagtacac tctctcgata
     6121 cgaataaacg gctcagaaat gagccgttta ttttttctac ccatatcctt gaagcggtgt
     6181 tataatgccg cgccctcgat atggggattt ttaacgacct gattttcggg tctcagtagt
     6241 agttgacatt agcggagcac taaaatgatc caagaacaga ctatgctgaa cgtcgccgac
     6301 aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg gtggctcgca ccgtcgctac
     6361 gcaggcgtag gcgacatcat caagatcacc atcaaagaag caattccgcg tggtaaggtc
     6421 aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca agaagggtgt tcgtcgcccg
     6481 gacggttctg tcattcgctt cgatggtaat gcttgtgttc ttctgaacaa caacagcgag
     6541 cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg agcttcgtag tgagaagttc
     6601 atgaaaatta tctctctggc accagaagta ctctaaggag cgaatcatgg cagcgaaaat
     6661 ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat aaaggtaaac gcggtaaagt
     6721 taagaatgtc ctgtcttccg gcaaggtcat tgttgaaggt atcaacctgg ttaagaaaca
     6781 tcagaagccg gttccggccc tgaaccaacc gggtggcatc gttgaaaaag aagccgctat
     6841 tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc aaggctgacc gtgtaggctt
     6901 tagattcgaa gacggcaaaa aagtccgttt cttcaagtct aacagcgaaa ctatcaagta
     6961 atttggagta gtacgatggc gaaactgcat gattactaca aagacgaagt agttaaaaaa
     7021 ctcatgactg agtttaacta caattctgtc atgcaagtcc ctcgggtcga gaagatcacc
     7081 ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac tgctggataa cgcagcagca
     7141 gacctggcag caatctccgg tcaaaaaccg ctgatcacca aagcacgcaa atctgttgca
     7201 ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag taactctgcg tggcgaacgc
     7261 atgtgggagt tctttgagcg cctgatcact attgctgtac ctcgtattcg tgacttccgt
     7321 ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca gcatgggtgt ccgtgagcag
     7381 atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg ttcgtggttt ggatattacc
     7441 attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc tgctggctgc ctttgacttc
     7501 ccgttccgca agtaaggtag ggttactaaa tggctaagca atcaatgaaa gcacgcgaag
     7561 taaaacgcgt agctttagct gataaatact tcgcgaaacg cgctgaactg aaagcgatca
     7621 tctctgatgt gaacgcttcc gacgaagatc gttggaacgc tgttctcaag ctgcagactc
     7681 tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg ccgtcaaaca ggtcgcccgc
     7741 atggtttcct gcggaagttc gggttgagcc gtattaaggt ccgtgaagcc gctatgcgcg
     7801 gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc accaattgaa tcacgggagt
     7861 aaagacagat gagcatgcaa gatccgatcg cggatatgct gacccgtatc cgtaacggtc
     7921 aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa gctgaaagtg gcaatcgcca
     7981 acgtgctgaa ggaagaaggt tttattgaag attttaaagt tgaaggcgac accaagcctg
     8041 aactggaact tactctgaag tatttccagg gcaaagctgt tgtagaaagc attcagcgtg
     8101 tcagccgccc aggtctgcgc atctataaac gtaaagatga gctgccgaaa gttatggcgg
     8161 gtctgggtat cgcagttgtt tctacctcta aaggtgttat gactgatcgt gcagcgcgcc
     8221 aggctggtct tggtggcgaa attatctgct acgtagccta atcggaggaa aaaatgtctc
     8281 gtgttgctaa agcaccggtc gttgttcctg ccggcgttga cgtaaaaatc aacggtcagg
     8341 ttattacgat caaaggtaaa aacggcgagc tgactcgtac tctcaacgat gctgttgaag
     8401 ttaaacatgc agataatacc ctgaccttcg gtccgcgtga tggttacgca gacggttggg
     8461 cacaggctgg taccgcgcgt gccctgctga actcaatggt tatcggtgtt accgaaggct
     8521 tcactaagaa gctgcagctg gttggtgtag gttaccgtgc agcggttaaa ggcaatgtga
     8581 ttaacctgtc tctgggtttc tctcatcctg ttgaccatca gctgcctgcg ggtatcactg
     8641 ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgt tgataagcag gtgatcggcc
     8701 aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc ttataaaggc aagggtgttc
     8761 gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa gaagtaaggt aacactatgg
     8821 ataagaaatt tgctcgtatc cgtcgtgcga cccgcgcacg ccgcaagctc caggagctgg
     8881 gcgcaactcg cctggtggta catcgtaccc cgcgtcacat ttacgcacag gtaattgcac
     8941 cgaacggttc tgaagttctg gtagctgctt ctactgtaga aaaagctatc gctgaacaac
     9001 tgaagtacac cggtaacaaa gacgcggctg cagctgtggg taaagctgtc gctgaacgcg
     9061 ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc cgggttccaa tatcatggtc
     9121 gtgtccaggc actggcagat gctgcccgtg aagctggcct tcagttctaa ggtagaggtg
     9181 taagatggct cacatcgaaa aacaagctgg cgaactgcag gaaaagctga tcgcggtaaa
     9241 ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc ttcacagctc tgactgtagt
     9301 tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa gcgcgtgaag ttccagcagc
     9361 gatccagaaa gcgatggaaa aagcccgtcg caatatgatt aacgtcgcgc tgaataacgg
     9421 cactctgcaa caccctgtta aaggtgttca cacgggttct cgcgtattca tgcagccggc
     9481 ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc gccgttctgg aagtcgctgg
     9541 ggttcataac gttctggcta aagcatatgg ttccaccaac ccgatcaacg tggttcgtgc
     9601 aactattgat ggcctggaaa atatgaattc tccagaaatg gtcgctgcca agcgtggtaa
     9661 atccgttgaa gaaattctgg ggaaataaac catggcaaag actattaaaa ttactcaaac
     9721 ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg ctgcttggcc tgggtctgcg
     9781 tcgtattggt cacaccgtag agcgcgagga tactcctgct attcgcggta tgatcaacgc
     9841 ggtttccttc atggttaaag ttgaggagta agagatgcgt ttaaatactc tgtctccggc
     9901 cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt atcggttctg gcctcggtaa
     9961 aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct ggcggtggcg tacgtcgcgg
    10021 tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg aaattcggct tcacttctcg
    10081 taaagcagcg attacagccg aagttcgtct gtctgacctg gctaaagtag aaggcggtgt
    10141 tgtagacctg aacacgctga aagcggctaa cattatcggt atccagatcg agttcgcgaa
    10201 agtgatcctg gctggcgaag taacgactcc ggtaactgtt cgtggcctgc gtgttactaa
    10261 aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc gaggaataag tagcagatgg
    10321 ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg cttaggcgag ctgaaacgca
    10381 gactgctgtt tgttatcggt gcgctgattg tgttccgtat tggctctttt attccgatcc
    10441 ctggtattga tgccgctgta cttgccaaac tgcttgagca acagcgaggc accatcattg
    10501 agatgtttaa catgttctct ggtggtgctc tcagccgtgc ttctatcttt gctctgggga
    10561 tcatgccgta tatttcggcg tcgatcatta tccagctgct gacggtggtt cacccaacgt
    10621 tggcggaaat taagaaagaa ggggagtctg gtcgtcgtaa gatcagccag tacacccgct
    10681 acggtactct ggtgctggca atattccagt cgatcggtat tgctactggt ctgccgaata
    10741 tgcctggtat gcaaggcctg gtgattaacc cgggctttgc attctacttc accgctgttg
    10801 taagtctggt cacaggaacg atgttcctga tgtggttggg cgaacagatt actgaacgag
    10861 gtatcggcaa cggtatttca atcattatct tcgtcggtat tgtcgcggga ctcccgccag
    10921 ccattgccca tactatcgag caagcgcgtc aaggcgacct gcacttcctc gtgttgctgt
    10981 tggttgcagt attagtattt gcagtgacgt tctttgttgt atttgttgag cgtggtcaac
    11041 gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg tcgtgtttat gctgcacaga
    11101 gcacacattt accgctgaaa gtgaatatgg cgggggtaat cccggcaatc ttcgcttcca
    11161 gtattattct gttcccggcg accatcgcgt catggttcgg gggcggtact ggttggaact
    11221 ggctgacaac aatttcgctg tatttgcagc ctgggcaacc gctttatgtg ttactctatg
    11281 cgtctgcaat catcttcttc tgtttcttct acacggcgtt ggttttcaac ccgcgtgaaa
    11341 cagcagataa cctgaagaag tccggtgcat ttgtaccagg aattcgtccg ggagagcaaa
    11401 cggcgaagta tatcgataaa gtaatgaccc gcctgaccct ggttggtgcg ctgtatatta
    11461 cctttatctg cctgatcccg gagttcatgc gtgatgcaat gaaagtaccg ttctacttcg
    11521 gtgggacctc actgcttatc gttgttgtcg tgattatgga ctttatggct caagtgcaaa
    11581 ctctgatgat gtccagtcag tatgagtctg cattgaagaa ggcgaacctg aaaggctacg
    11641 gccgataatt ggtcgcccga gaagttacgg agagtaaaaa tgaaagttcg tgcttccgtc
    11701 aagaaattat gccgtaactg caaaatcgtt aagcgtgatg gtgtcatccg tgtgatttgc
    11761 agtgccgagc cgaagcataa acagcgccaa ggctgatttt ttcgcatatt tttcttgcaa
    11821 agttgggttg agctggctag attagtcagc caatcttttg tatgtctgtg cgtttccatt
    11881 tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca tattaaatag taggagtgca
    11941 tagtggcccg tatagcaggc attaacattc ctgatcataa gcatgccgta atcgcattaa
    12001 cttcgattta tggcgtcggc aagacccgtt ctaaagccat cctggctgca gcgggtatcg
    12061 ctgaagatgt taagatcagt gagctgtctg aaggacaaat cgacacgctg cgtgacgaag
    12121 ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat cagcatgagc atcaagcgcc
    12181 tgatggatct tggttgctat cgcggtttgc gtcatcgtcg tggtctcccg gttcgcggtc
    12241 agcgtaccaa gaccaacgca cgtacccgta agggtccgcg caaaccgatc aagaaataat
    12301 cggggtgatt gaataatggc aaaggcacca attcgtgcac gtaaacgtgt aagaaaacaa
    12361 gtctctgacg gcgtggctca tatccatgct tctttcaaca acaccatcgt gactatcact
    12421 gatcgtcagg gtaacgcgtt gggttgggca acagccggtg gttccggttt ccgtggttct
    12481 cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc gttgcgctga cgccgtgaaa
    12541 gaatacggca tcaagaatct ggaagttatg gttaaaggtc cgggtccagg ccgcgaatct
    12601 actattcgtg ctctgaacgc cgcaggtttc cgcatcacta acattactga tgtgactccg
    12661 atccctcata acggttgtcg tccgccgaaa aaacgtcgcg tataacgcct cgttttccag
    12721 gtttgttgga gaaagaaaat ggcaagatat ttgggtccta agctcaagct gagccgtcgt
    12781 gagggcaccg acttattcct taagtctggc gttcgcgcga tcgataccaa gtgtaaaatt
    12841 gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc tgtctgacta tggtgtgcag
    12901 ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc tggagcgtca gttccgtaac
    12961 tactacaaag aagcagcacg tctgaaaggc aacaccggtg aaaacctgtt ggctctgctg
    13021 gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg gtgccactcg tgcagaagca
    13081 cgtcagctgg ttagccataa agcaattatg gtaaacggtc gtgttgttaa catcgcttct
    13141 tatcaggtta gtccgaatga cgttgtaagc attcgtgaga aagcgaagaa gcagtctcgc
    13201 gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc caacctggct ggaagttgat
    13261 gctggcaaga tggaaggtac gtttaagcgt aagccggagc gttctgatct gtctgcggac
    13321 attaacgaac acctgatcgt cgagctttac tccaagtaaa gcttagtacc aaagagagga
    13381 cacaatgcag ggttctgtga cagagtttct aaaaccgcgc ctggttgata tcgagcaagt
    13441 gagttcgacg cacgccaagg tgacccttga gcctttagag cgtggctttg gccatactct
    13501 gggtaacgca ctgcgccgta ttctgctctc atcgatgccg ggttgcgcgg tgaccgaggt
    13561 tgagattgat ggtgtactac atgagtacag caccaaagaa ggcgttcagg aagatatcct
    13621 ggaaatcctg ctcaacctga aagggctggc ggtgagagtt cagggcaaag atgaagttat
    13681 tcttaccttg aataaatctg gcattggccc tgtgactgca gccgatatca cccacgacgg
    13741 tgatgtcgaa atcgtcaagc cgcagcacgt gatctgccac ctgaccgatg agaacgcgtc
    13801 tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat gtgccggctt ctacccgaat
    13861 tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg gtcgacgcat gctacagccc
    13921 tgtggagcgt attgcctaca atgttgaagc agcgcgtgta gaacagcgta ccgacctgga
    13981 caagctggtc atcgaaatgg aaaccaacgg cacaatcgat cctgaagagg cgattcgtcg
    14041 tgcggcaacc attctggctg aacaactgga agctttcgtt gacttacgtg atgtacgtca
    14101 gcctgaagtg aaagaagaga aaccagagtt cgatccgatc ctgctgcgcc ctgttgacga
    14161 tctggaattg actgtccgct ctgctaactg ccttaaagca gaagctatcc actatatcgg
    14221 tgatctggta cagcgtaccg aggttgagct ccttaaaacg cctaaccttg gtaaaaaatc
    14281 tcttactgag attaaagacg tgctggcttc ccgtggactg tctctgggca tgcgcctgga
    14341 aaactggcca ccggcaagca tcgctgacga gtaaccggat cacaggttaa ggttttactg
    14401 agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca actgaaccgc aacagcagcc
    14461 atcgccaggc tatgttccgc aatatggcag gttcactggt tcgtcatgaa atcatcaaga
    14521 cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga gccgctgatt actcttgcca
    14581 agactgatag cgttgctaat cgtcgtctgg catttgcccg tactcgtgat aacgagatcg
    14641 tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag ccgtgccggt ggttacactc
    14701 gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc gatggcttac atcgagctgg
    14761 ttgatcgttc agagaaagca gaagctgctg cagagtaatc tgaagcaatg taaaaaaacc
    14821 cgccccggcg ggttttttta tacccgtagt atccccactt atctacaata gctgtactct
    14881 ttttgttcat cccctggagt atttatgtgg ttacttgacc agtgggcaga gcgccatata
    14941 gcagaagcgc aagcgaaagg tgagtttgat aacctggcag gtagcggcga accattgata
    15001 ctggatgatg attctcacgt gccaccggaa ttacgtgcgg ggtatcgctt gctgaagaat
    15061 gccggttgct taccgccaga acttgagcaa cggagagaag caattcagct tctggatatt
    15121 ctcaaaggta tccgtcacga tgatccgcaa tatcaagagg ttagccgtcg attgtcatta
    15181 ctggaattga agctgcgaca agctggattg agtaccgatt ttttacgcgg cgattatgct
    15241 gacaagttgt tggacaaaat caacgataac tagtggagta tgtatgtatc gcattggtga
    15301 gctggcaaaa atggcggaag taacacctga cacgattcgt tattacgaaa aacagcagat
    15361 gatggagcat gaagtgcgta ctgaaggcgg gtttcgcctg tataccgaaa gcgatctcca
    15421 gcgattgaaa tttatccgcc atgccagaca actaggtttc agtctggagt cgatccgcga
    15481 gttgctgtcg atccgcatcg atcctgaaca ccatacctgt caggagtcaa aaggcattgt
    15541 gcaggaaaga ttgcaggaag tcgaagcacg gatagccgag ttgcagagta tgcagcgttc
    15601 cttgcaacgc cttaacgatg cctgttgtgg gaccgcccat agcagtgttt attgctcgat
    15661 tcttgaagct cttgaacaag gggcgagtgg cgttaagagt ggctgttgat tttttgcact
    15721 gacaggatta cactcgcgcc gttaaataac caactggagt ttttatgagc cgatatcagc
    15781 atactaaagg gcagataaag gataatgcga tagaagcatt actacatgat cccttattcc
    15841 gacagcgagt agagaaaaat aagaaaggga aaggcagtta catgcgaaaa ggaaaacatg
    15901 gcaatcgggg gaactgggag gccagtggca agaaagtaaa tcactttttt accactggtc
    15961 ttctgctttc aggcgcttgt taagagcggt tattctgctc tttcagcaaa tcacgaattt
    16021 ctgccagtaa tacttcttct ttagttggtg caggtgcggc agctggttct tcttttttcc
    16081 gattcagttt gttgattagc ttaatcgcca taaagatggc aaaggccaca atcagaaaat
    16141 caaagacgtt ttgaatgaag acaccgtaat gcatcacaac agcagggata tccccctgcg
    16201 catcgcgtag cgtgacagca aactgtttaa agtcgatccc gccaattaat aagcccagag
    16261 gtggcatgat gatatcggca accagtgaag atacaatctt cccgaatgcc gcaccgataa
    16321 tgacacccac cgccaaatcc accacgttcc cgcgcatcgc aaattcgcga aattctttaa
    16381 taatgctcat gttattctcc ctatgaagcc gacaaccata agtctaacaa atgttaagcc
    16441 attttcctgc caggaatgaa tttaattaat ccttgataat caaagggaaa taataaggcg
    16501 tcattagacg ccttattaat tacaagaaga aagggcttgg ctggaagagt ctttcgacgt
    16561 cggtaataaa ctttttatct gtgaggaaca taattacgtg atcgccttgc tcaatgcgca
    16621 gattgtcatt ggcaatcatc acgtcgtttc cacgtaccac cgctccaata atcgttcctg
    16681 gcggtagctt gatttcgtca atgactctgc cgacaacgcg tgaggtgctt tcatcaccgt
    16741 gagcaacggc ttcaatagct tctgctacgc cgcggcgcaa tgaggaaaca ccaacaatat
    16801 ctgcttttcg cacatggcta agcaacgcag aaatagttgc ttgttgtggt gaaattgcaa
    16861 tatcgatcac gctcccctga accagatcca cataagcgcg acgctggatc aataccatca
    16921 cctttttcgc acccatacgt ttggcaagca tggcggacat gatattggcc tcgtcatcgt
    16981 tggtgacggc aataaacaga tcaacttgat cgatatgttc ttcggccagc agttcttgat
    17041 ccgacgcatc accaaaaaag acgatcgtat tctgcaactt ttccgccagt tcggcagcgc
    17101 gctgctgatt acgttcgatg agtttaacgc tgtaatcttt ttccagacga cgcgccagcc
    17161 ctgcaccgat attaccgcca ccaaccagca tgatccgctt atacggtttt tccagtcgct
    17221 gtaattcact catcaccgcg cggatatgct gtgaagcggc aataaagaac acttcatcac
    17281 cagcttcaac aatggtcgaa ccttgtggac gaatggggcg atcgtggcgg aaaattgctg
    17341 ccacacgagt atcgatatgt ggcatatgtt cgcgcatggt cgaaagtgca ttaccaatca
    17401 gcgggccgcc ataataggct ttaactacag cgaggctgac tttaccctca gcgaagttca
    17461 ccacctgcaa tgcgcctgga tactcaatca gtcggtaaat attatcgata accaactgct
    17521 ctggtgcgat cagatgatca atcggtacag catctgaatg aaatagctta tcggcatcgc
    17581 gtacgtagtc tggtgagcgg atgcgagcga tgcgattagg ggtgttgaaa agtgagtagg
    17641 ctacctggca ggcaaccata ttggtttcat ctgaactggt tacagcaacc agcatatcgg
    17701 cgtcgtcggc acctgcctcc cgcaatacgc gtggatgaga gccatgcccc tgcacgactc
    17761 gcaggtcaaa tttatcctgc aaggtccgca gacgctcacc gttggtatcg acaacagtaa
    17821 tatcgttgtt ctcgccaacc aggttttccg ccagtgtgcc gccaacctgg ccggcaccca
    17881 gaatgataat tttcatcagt ctcgacccgt tatctcatca ctttttgatt agcttagcgt
    17941 aaaagaagcc gtcgccctct tcggcaccag gcagattttg tttacccggt tgctctggtg
    18001 ttcctgtttc gcaaagttcg gcatcagcgg tacgttgcaa aaaagcttta atctgcaggc
    18061 tattctcttc cggtaacacc gaacaggtgg catagaccag ggttccaccg gattttaaat
    18121 gcggccaaat ggcgtcgaga atttcagact gcaattgcgc gagttccggg atatcgcgat
    18181 cgcgacgtaa ccatttaata tccggatggc gacgaatcac ccccgttgct gaacaaggcg
    18241 catctaataa aatgcgatca aactgttgct cgccacacca ttgggaaggg taacggccat
    18301 caccttgttt cacggtcgcc ttcataccaa ggcgttttaa attgtcgtga acgcgagaga
    18361 ggcgctgttc gtcaatatca accgcaacaa cctgcgcttc tggtgccacc tcaaggatat
    18421 gcgttgtttt accgccgggg gcggcacaaa gatccagaat gtgttcaccg ttttgtggcg
    18481 caagccaggt catgcaacct tgtgctgatg catcctgaac ggtaacccat ccgtcttcaa
    18541 aaccaggtag cgcatgaaca ggtgcaggtg tttccagccg tacagcatca gggtaatccg
    18601 catgcgggaa acctttcatt cctgcttcat ccaacaatgc aagccagctg tcgcgggaat
    18661 gatgcgtacg attaacgcgc agccacattg gcggacgctg gttattggct tcgacgatgg
    18721 attgccactg ctctggatac gctttttgca gacgcttcag caaccaggaa ggatgcagat
    18781 aacgtgcatc actggtatta aactcggcta ataactcttc ttgctgacgc tggaactggc
    18841 gtaatacacc gttaatcaac cctttaagtt gcggacgctt aattgcgata gcgccttcaa
    18901 ccgtttcagc cagcgcagca tgaggtggaa tgcgggtata aagcagttga tacaaaccaa
    18961 ccataatcag gtaatgcaca gtgcgctgtt tgccggtcat cggacgggcc attaacttat
    19021 taatcagcca gtctaactgc gaaagcgtac gcagtacgcc aaagcacaac tcttgaagaa
    19081 gtgctttgtc tttatcagaa actttttgct ggagcggtgg cagaatgttg cttaatgatt
    19141 gcccttgctc gacgacttgt tcaacggcct gggccgccat gctacgtaaa ttacgttgtt
    19201 ttttcataag tataaaaatg cccggtaaga ccgggcttag aagagtggac tatcaggcca
    19261 gacggttgcc cggaacaaac cattcccgac gagaattcag gaggtcttgc gcgctcatcg
    19321 ctttcttacc cgcaggttgt aacgagagca ggttcaggat gccatcacca gtcgcaacct
    19381 gaatgccttg tttgttggct tcaaggatcg ttcctggtgc agcgttggtt gccgtatcaa
    19441 tgaccgacgc tttccagact ttaaccggtt gtccttcaat ttccagccag ctcattggcc
    19501 atgggttgaa agcgcgaatg cagcgttcaa gctgcgctgc cgaaagcgac cagtcaatac
    19561 gcgcttcttc tttactcaac ttctcggcgt aagtgacaag agtttcgtcc tgaacttctg
    19621 gtttcgccgt gccgtctgct agttgtttca acgtggtgat tagcccttgt gggccaagct
    19681 ctgccagctt gtcgtacagc gtaccactgg tatcttctgc agtaatcggg caggagagct
    19741 tatagagcat atcaccggtg tctaaaccga catccatttg cataatggtc acaccagttt
    19801 ctgcatcacc cgcccatagt gagcgttgga ttggtgcagc accgcgccag cgtggcagca
    19861 gtgaaccatg aacgttgata cagccaagac gcggcatctc cagcactgct ttaggcagaa
    19921 ttaagccata ggcgacgacg accataacat cagcctgcag atcggcgacc agttgctggc
    19981 tttcttgtgg acgcagggaa acaggttgaa aaacgggcag acctttttcc tcagccagaa
    20041 ctttaaccgg gctgggcatc agttttttac cgcgtcctgc cggtcggtct ggctgggtga
    20101 acacgccaac gacgttatga ccagaagaca acagcgcgtc gagatgacgc gctgcaaagt
    20161 caggtgtacc cgcaaaaata atacgtagtg attctgacac gttagttctt atccttaagc
    20221 ccgggctttc agacgatcta gtttttcaac tttctgacga atacgttgtt gtttcagcgg
    20281 tgacagataa tccataaaca gtttgccgac caggtgatcc atctcatgct gaatacagat
    20341 ggctaacagc ccctctgctt ccagttcaaa tggtttaccg tcgcggtcaa gggcgcgaat
    20401 tttaactttc tctgcgcgcg gcactaaagc acgttgttca gggatcgaca ggcaaccttc
    20461 ttcaatgcct gtttcgccgc ttttttctaa aagctccgga ttgattaaca ccagccgttc
    20521 gtcacggttt tccgaaacat caataacaat gatacgttga tggatatcaa cctgggttgc
    20581 cgccaggcca ataccttctt ctgcgtacat cgtctcgaac atatcatcga cgatacgctg
    20641 tatttctgca ttcacttctt ctaccggttt agcaactttg cgaagccgct cgtccggaat
    20701 atgtaacact tgcaaaactg acataaatct ccagagatgt gttcaggggt tagaaagatt
    20761 atttcttcta ttctagacaa atccccctct gattgacagc atcactgacc aatcgcaaag
    20821 attgctaagg ctgcttatgg cagggagata aggatggtcg atacagaaat ttggctgcgt
    20881 ttaatgagta tcagcagctt gtacggcgat gatatggtcc gtatagctca ctggctggca
    20941 aaacagtcgc aaattgatgc ggttggattg cagcaaacag ggcttacatt gcggcaggca
    21001 caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg gttggagcaa
    21061 cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact tctggtgacg
    21121 acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca ttcatttcag
    21181 cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg acggttattt
    21241 tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg tggaatcgat
    21301 ggtgtggcgc ataaagctgc cttacaggta aatggcgtca gcattgctgt attggggaat
    21361 ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct gcttgaacat
    21421 ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta caatttccca
    21481 cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga agcggctttg
    21541 cgcagtggtt cgctggtgac agcacgttgt gcgcttgagc agaggcgtga agtttttgcc
    21601 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    21661 gcgattcttg tgacggaacc ggaagaaatt ctggaaaact tgcaatttgg attgcactgg
    21721 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    21781 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    21841 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    21901 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    21961 actaatgtat ttgtttgaaa cctatattca cactgaagct gagttgcgtg tggatcaaga
    22021 caaacttgaa caggatctta ccgacgcagg ttttgagcga gaagatatct acaatgccct
    22081 gctatggctg gaaaaacttg ctgattatca ggaagggttg gcagaaccga tgcaactggc
    22141 ctcggatcct ctctccatgc gtatttatac accggaagag tgtgaacgac tggatgccag
    22201 ctgccgtggg tttctgcttt tccttgagca gattcaggtg ctcaaccttg aaactcgtga
    22261 aatggtgata gagcgagtgc tcgcgctgga taacgcagag ttcgaactgg atgatctgaa
    22321 atgggtgatc ctgatggtgt tgttcaacat cccggggtgc gaaaatgcgt accagcaaat
    22381 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    22441 cataagttgt tatggcaaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    22501 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    22561 agtatccggc gtgtgactac gtccgtcttc tgaaatcttc agcggatgga catattgtca
    22621 aagttctgga ggggcaggtt tgccctgcat gtggcgcaaa tctggtatta cgccagggac
    22681 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    22741 ataaaccgga cgaaacagca attacatgcc cccaatgtcg gacgggccat ctggtccagc
    22801 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    22861 ccattaactt caaaccaata gctggagaat gccctgagtg tcattatccg ctactcatcg
    22921 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    22981 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23041 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23101 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    23161 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    23221 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    23281 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    23341 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    23401 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    23461 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    23521 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    23581 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    23641 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    23701 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    23761 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    23821 tggtgcagtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    23881 tggtgtaggc ttgttaagcg atctggaacg tctgtctttt atccgccctg gtttacgtat
    23941 tctgcttatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctaga
    24001 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24061 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24121 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    24181 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    24241 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    24301 ggcacaggcg gctcatgcct tccttctttg gcatggcgtt ctgcctgatg tagaaccggt
    24361 catcaaacta ttgcaacagg aattgtcagc gtgaatcagg ccatccagtt tccggatagg
    24421 gaagagtggg acgagaataa aagatgtgta tgttttcctg ctctcgtgaa tggtatgcaa
    24481 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgct ttactggagg tacgccagaa
    24541 cagtggttag cgagttttcg tcagcatcgc tgggacctgg aagaagaagc ggaaaactta
    24601 attcaggaac aaagtgaaga tgatcaaggt tgggtctggt taccttgatc cagatattcg
    24661 tccttccatt tcacgtagtt atttgcggaa tagcgtaacc cagccttctc ttcatcactt
    24721 aacgggcgga tctgtttgac gggactaccg agatacagat atccgctctc cagccgttta
    24781 ttttgtggga ccaggctacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    24841 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    24901 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    24961 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25021 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25081 agacgaacgt caccaatcac gacgctgcta tcgtcgatca ttacgcgctg gccgatttgt
    25141 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac tcacctcaac aataaatgat
    25201 ttcctaatga ctttgggtgc attattagcc ttgtgcaagt cttttagtat gcaaaaaagc
    25261 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    25321 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    25381 gtgaaacact tcacaggatg gtcggaacaa cgaagagaaa aaattctgaa attcagggtt
    25441 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaag
//
LOCUS       DAZSBF010000041        25104 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZSBF010000041 DAZSBF010000000
VERSION     DAZSBF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.041"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            194..382
                     /gene="bcsR"
                     /locus_tag="ACHXWD_001309"
     CDS             194..382
                     /gene="bcsR"
                     /locus_tag="ACHXWD_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR7620277.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHXWD_001310"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="ACHXWD_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR7620278.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="ACHXWD_001311"
     CDS             1143..1307
                     /locus_tag="ACHXWD_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR7620279.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHXWD_001312"
                     /pseudo
     CDS             1314..3761
                     /gene="bcsA"
                     /locus_tag="ACHXWD_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHXWD_001313"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="ACHXWD_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR7620280.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHXWD_001314"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="ACHXWD_001314"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR7620281.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHXWD_001315"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="ACHXWD_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHXWD_001316"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="ACHXWD_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR7620282.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="ACHXWD_001317"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="ACHXWD_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR7620283.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="ACHXWD_001318"
     CDS             14457..15953
                     /locus_tag="ACHXWD_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR7620284.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHXWD_001319"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="ACHXWD_001319"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR7620285.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHXWD_001320"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="ACHXWD_001320"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR7620286.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="ACHXWD_001321"
     CDS             18047..20107
                     /locus_tag="ACHXWD_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR7620287.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="ACHXWD_001322"
     CDS             complement(20289..21611)
                     /locus_tag="ACHXWD_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7620288.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHXWD_001323"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="ACHXWD_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR7620289.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="ACHXWD_001324"
     CDS             complement(23084..23983)
                     /locus_tag="ACHXWD_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7620290.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="ACHXWD_001325"
     CDS             24503..>25104
                     /locus_tag="ACHXWD_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR7620291.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6365 c         6991 g         5854 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagctgga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAZSBF010000042        24527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZSBF010000042 DAZSBF010000000
VERSION     DAZSBF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.042"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(166..576)
                     /locus_tag="ACHXWD_001326"
     CDS             complement(166..576)
                     /locus_tag="ACHXWD_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR7620292.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(634..867)
                     /locus_tag="ACHXWD_001327"
     CDS             complement(634..867)
                     /locus_tag="ACHXWD_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR7620293.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1256..1801
                     /locus_tag="ACHXWD_001328"
     CDS             1256..1801
                     /locus_tag="ACHXWD_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR7620294.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1776..3701
                     /locus_tag="ACHXWD_001329"
     CDS             1776..3701
                     /locus_tag="ACHXWD_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR7620295.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3698..3904
                     /locus_tag="ACHXWD_001330"
     CDS             3698..3904
                     /locus_tag="ACHXWD_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR7620296.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3901..5502
                     /locus_tag="ACHXWD_001331"
     CDS             3901..5502
                     /locus_tag="ACHXWD_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR7620297.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5483..6802
                     /locus_tag="ACHXWD_001332"
     CDS             5483..6802
                     /locus_tag="ACHXWD_001332"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR7620298.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6812..7144
                     /locus_tag="ACHXWD_001333"
     CDS             6812..7144
                     /locus_tag="ACHXWD_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR7620299.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            7200..8225
                     /locus_tag="ACHXWD_001334"
     CDS             7200..8225
                     /locus_tag="ACHXWD_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR7620300.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            8267..8665
                     /locus_tag="ACHXWD_001335"
     CDS             8267..8665
                     /locus_tag="ACHXWD_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR7620301.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8677..9030
                     /locus_tag="ACHXWD_001336"
     CDS             8677..9030
                     /locus_tag="ACHXWD_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR7620302.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9042..9620
                     /locus_tag="ACHXWD_001337"
     CDS             9042..9620
                     /locus_tag="ACHXWD_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7620303.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9617..10012
                     /locus_tag="ACHXWD_001338"
     CDS             9617..10012
                     /locus_tag="ACHXWD_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR7620304.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            10020..10760
                     /locus_tag="ACHXWD_001339"
     CDS             10020..10760
                     /locus_tag="ACHXWD_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7620305.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10776..11198
                     /locus_tag="ACHXWD_001340"
     CDS             10776..11198
                     /locus_tag="ACHXWD_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR7620306.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            11180..11614
                     /locus_tag="ACHXWD_001341"
     CDS             11180..11614
                     /locus_tag="ACHXWD_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR7620307.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11607..14168
                     /locus_tag="ACHXWD_001342"
     CDS             11607..14168
                     /locus_tag="ACHXWD_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7620308.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            14165..14494
                     /locus_tag="ACHXWD_001343"
     CDS             14165..14494
                     /locus_tag="ACHXWD_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7620309.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14494..15192
                     /locus_tag="ACHXWD_001344"
     CDS             14494..15192
                     /locus_tag="ACHXWD_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR7620310.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15198..15941
                     /locus_tag="ACHXWD_001345"
     CDS             15198..15941
                     /locus_tag="ACHXWD_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR7620311.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15938..16510
                     /locus_tag="ACHXWD_001346"
     CDS             15938..16510
                     /locus_tag="ACHXWD_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR7620312.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16571..20068
                     /locus_tag="ACHXWD_001347"
     CDS             16571..20068
                     /locus_tag="ACHXWD_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR7620313.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            20139..20655
                     /locus_tag="ACHXWD_001348"
                     /pseudo
     CDS             20139..20655
                     /locus_tag="ACHXWD_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            20720..23803
                     /locus_tag="ACHXWD_001349"
     CDS             20720..23803
                     /locus_tag="ACHXWD_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7620314.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQH
                     SQTGPRTNSGSQPTGMFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            23815..24384
                     /locus_tag="ACHXWD_001350"
     CDS             23815..24384
                     /locus_tag="ACHXWD_001350"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7620315.1"
                     /translation="MSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTDIAPP
                     DIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSPEGEF
                     QKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKETLLL
                     EAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT         5816 a         6115 c         7449 g         5147 t
ORIGIN      
        1 gtcgccgtat tcccgtatta acagagaccg tagcccgacg gggaactcct tctgcgcgag
       61 tgtgcgggaa taatcaaaaa cgatgcacac cggggttacc gggtacacat atttcatcat
      121 gccagcgagt ccggttctgg cacggaagaa accggacgtt atgatttagt gcggaaatat
      181 ttgtgtagtg ttctgtatgt tctcagtaaa gagtaatgaa ttatcaaagg tatagtaata
      241 ccttttgttt tcgtggatat ttgtaatcca tctgaaaacc cctgctgtag caagattttt
      301 cctgtattcg taaaatgata actctcctga tttgaatcct tttaaggtgg cttctataag
      361 gcatttattt tttgaaaatc ttacatttac aaccttaccc tgtcctttta ttaaaaccgt
      421 attatcgttt tcaagaacaa gatgaatatt ctctgtggct aaatagtaaa tgtaatgtga
      481 gacattgtga cgttttagtt cagaataaaa ccagtgatag tttaaattat ttcgcacttt
      541 atcgaatatt tgtttaaaaa tggcaacctg agccattgta gtaccttcca tgtgatatga
      601 gggggcgtag tctgcacgat tatctaaatt gcttcaatct ggtctaacct gttttctgag
      661 caattcagta atgtcactct tttctttgtt tgcttcaggc gaaactcttt tttctgagca
      721 cagtctccgg cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgaac
      781 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
      841 tctgcgggtt ctgttcttcg ttgacatgag gttgccctgt attcagtgtc gctgatttgt
      901 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
      961 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgacat gtagatgata
     1021 attattatca ttttgcgggt cctttccggc gatccgacag gttacggggc ggaaggcgcg
     1081 cgggttttcg ctatttatga caattttccg gtttaaggcg tttccgttct tcttcgccgt
     1141 aacctaatgt ttttatttaa aacaccccct gaaaagaaag gaaacgacag gtgctgaaaa
     1201 cgggcttttt ggcctctgtc gtttcctttc tctgtttttg tccgtggaat gaacaatgga
     1261 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     1321 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     1381 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     1441 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     1501 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     1561 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     1621 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     1681 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     1741 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     1801 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     1861 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     1921 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     1981 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     2041 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     2101 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     2161 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     2221 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     2281 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     2341 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     2401 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     2461 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     2521 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     2581 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     2641 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     2701 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     2761 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     2821 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     2881 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     2941 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagcgggat cgctacgaaa
     3001 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     3061 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     3121 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     3181 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     3241 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     3301 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     3361 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     3421 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     3481 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     3541 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     3601 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     3661 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     3721 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     3781 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     3841 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     3901 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     3961 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     4021 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     4081 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagctgca tcaggatcat
     4141 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     4201 gaggaagacg cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     4261 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     4321 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagt
     4381 tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     4441 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     4501 gcgctgggat attacgtcag cgaggacggc tatcctggct ggatgccgca gaaatggaca
     4561 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     4621 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg taatggagca gatgaagatg
     4681 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     4741 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     4801 aaggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     4861 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     4921 ctgcagacgg ctcaggacac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     4981 tatatcgctg ccgggctggg tgtctcgtat gagcaacttt cccggaatta cgcccagatg
     5041 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     5101 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggctgga agaggccatc
     5161 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     5221 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     5281 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     5341 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     5401 cgcgcagctg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cgggctgcga
     5461 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     5521 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     5581 gcttgcaggc cagcttggga tcagctgcct gacggatgca gtatccggcg acagcctgac
     5641 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     5701 ccgcagttat caggtaatga acggcatcgc cgtgctgccg gtgtccggca cgctggtcag
     5761 ccggacgcgg gcgctgcagc cgtactcggg gatgaccggt tacaacggca ttatcgcccg
     5821 tctgcaacag gctgccagcg acccgatggt ggacggcatt ctgctcgata tggacacacc
     5881 gggcggaatg gtggcgggag catttgactg cgctgacatc atcgcccgtg tgcgtgacat
     5941 aaagccggta tgggcgctgg ccaatgacat gaactgcagc gcaggtcagc tgcttgccag
     6001 tgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     6061 ggctcacagt aattacggcg ctgcactgga gaaacagggc gtggaaatca cgctgattta
     6121 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     6181 aacattgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     6241 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     6301 ggccattgat gccggactgg ctgatgaact tgttaacagt accgatgcga tcaccgtcat
     6361 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     6421 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactggcg tggtgcaagc
     6481 gacggagggc gagaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgcagc
     6541 ggttgcggca gaaaacagtc gcattatggg gatcctcaac tgtgaggagg ctcacggacg
     6601 cgaagaacag gcacgcgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     6661 tattctggcc gcagcaccac agagtgcaca gacgcgcagt gacactgcgc tggatcgtct
     6721 gatgcagggg gcaccggcac cgctggctgc aggtaacccg gcatctgatg ccgttaacga
     6781 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     6841 ccagccgctg ggcaatagtg acccggctca taccgcaacc gcgcccggcg gattgagtgc
     6901 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     6961 ggatggcacc accgacggtg ctgccgttgg cattcttgcg gttgctgctg accagaccag
     7021 caccacactg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     7081 ggctgccagc gacgagacga aaaaacggac cgtgtttgcc ggaacggcaa tcagcatcgt
     7141 ttaactttac ccttcatcac taaaggccgc ctgtgtggct ttttttacgg gattttttta
     7201 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     7261 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt cactacggag aaagtctatc
     7321 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     7381 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     7441 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
     7501 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
     7561 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc cgtgctcaag ggcaaataca
     7621 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
     7681 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
     7741 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
     7801 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt caaggagaag ctggataccc
     7861 gtcgcggctc tcattccgag ctggagacag cggtaaaaga cctgggcaaa gcggtgtcct
     7921 ataagggaat gtatggcgat gtggccatcg tcgtgtattc cggacagtac gtggaaaacg
     7981 gcgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggctcgcg
     8041 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggc attaacgctt
     8101 ctgcccgtta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
     8161 ttcagtcagc accgctgatg ctgctggctg atcctgatga gttcgtgtcc gttcaactgg
     8221 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagttcatga cgaaagatga
     8281 actgattgcc cgtcttcagg agctgggtga gcaattgaac cgcgatgtca gtttgacggg
     8341 aacgaaagaa gaactggcgc ttcgtgtggc agagctggaa gaagagcttg atgacacgga
     8401 tgaaactgcc ggtcaggaca cccctctcag ccgggaaaat gtgctgaccg ggcatgaaaa
     8461 tgaggtggta tcagcgcagc cggataccgt gattcaggat acggctgaac tggtcacggt
     8521 cgtggcactg gtgacgctgc atactgatgc acttcacgcc acgcgggata aacctgtggc
     8581 atttgtgctg ccgggaacgg cgtttcgtgt ctctgccggt gtggcagccg aaatgacaga
     8641 gcgcggcctg gccagaatgc aataacggga ggcgctgtgg ctgatttcga taacctgttc
     8701 gatgctgcca ttgcccgcgc cgatgaaacg atacgcgggt acatgggaac gtcagccacc
     8761 atgacatccg gtgagcagtc aggtgcggtg atacgtggtg tttttgatga ccctgaaaat
     8821 atcagctatg ccggacaggg cgtgcgcgtt gaaggctcca gcccgtccct gtttgtccgg
     8881 actgatgagg tgcggcagct gcggcgtgga gacacgctga ccatcggtga ggaaaatttc
     8941 tgggtagatc gggtttcgcc ggatgatggc ggaagttgtc atctctggct tggacggggc
     9001 gtaccgcctg ccgttaaccg tcgccgctga aagggggatg tatggccata aaaggtcttg
     9061 agcaggccgt tgaaaacctc agccgtatca gcaaaacggc ggtgcccggt gccgccgcaa
     9121 tggccattaa ccgcgttgct tcatccgcga tatcgcagtc tgcgtcacag gttgcccgtg
     9181 agacaaaggt acgccggaaa ctggtaaagg aaagggccag gctgaaaagg gccacggtca
     9241 aaaatccgca ggccagaatc aaagttaacc ggggggattt gcccgtaatc aagctgggta
     9301 atgcgcgggt tgtcctgtcc cgccgcaggc gtcataaaaa ggggcagcgt tcatccctga
     9361 aaggtggcgg cagcgtgctt gtggtgggta accgtcgtat tcccggcgcg tttattcagc
     9421 aactgaaaaa cggccggtgg catgttatgc agcgtgtggc cgggaaaaac cgttacccca
     9481 ttgatgtggt gaaaatcccg atggcggtgc cgctgaccac ggcgtttaaa cagaatattg
     9541 aacggatacg gcgtgaacgt cttccgaaag agctgggcta tgcgctgcag catcaactga
     9601 gaatggtaat aaagcgatga aacatactga actccgtgca gccgtactgg atgcactgga
     9661 gaagcatgac accggggcga cgctttttga tggtcgcccc gctgtttttg atgaggaaga
     9721 ttttccggca attgccgttt atctcaccgg cgctgaatac acgggcgaag agctggacag
     9781 cgatacctgg caggcggagc tgcatattga agttttcctg cctgctcagg tgccggattc
     9841 agagctggat tcgtggatgg agtcccggat ttatccggtg atgagcgata tcccggcact
     9901 gtcagatttg atcaccagta tggtggccag cggctatgac taccggcgcg acgatgatgc
     9961 gggcctgtgg agttcagccg atctgactta tgtcattatc tatgaaatgt gaggacgcta
    10021 tgcctgtacc aaatcctaca atgccggtga aaggtgccgg gaccaccctg tgggtttata
    10081 aggggagcgg tgacccttat gcgaacccgc tttcagacgt tgactggtcg cgtctggcaa
    10141 aagttaaaga cctgacgccc ggcgaactga ccgctgagtc ctatgacgac agctatctcg
    10201 atgatgaaga tgcagactgg actgcgaccg ggcaggggca gaaatctgcc ggagatacca
    10261 gcttcacgct ggcgtggatg cccggagagc aggggcagca ggcgctgctg gcgtggttta
    10321 atgaaggtga tacccgtgcc tataaaatcc gcttcccgaa cggcacggtc gatgtgttcc
    10381 gtggctgggt cagcagtatc ggtaaggcgg tgacggcgaa ggaggtgatt acccgcacgg
    10441 tgaaggtcac caatgtggga cgtccgtcga tggcagaaga tcgcagcacg gtgacggcga
    10501 cgaccggcat gacggtgaca cccgccagtg cttccgtagt gaaagggcag agcaccacgc
    10561 tgaccgtggc attccagccg gatggcgcaa ccgacaagag cttccgtgcg gtgtctgcgg
    10621 ataaaacaaa agccaccgtg tcggtcagtg gtatgaccat caccgtgaaa ggtgttgctg
    10681 caggcaaggt caacattccg gtcgtatctg gtaatggtga gtttgctgcg gttgcagaaa
    10741 tcaacgtcac cgccagttaa tccggagagt cagcgatgtt cctgaaaacc gaatcatttg
    10801 aacataacgg tgtgaccgtc acgctttctg aactgtcagc cctgcagcgt attgagcatc
    10861 tcgacctgat gaaacggcag gcagaacagg cggagtcaga cagcaaccgg aagtttactg
    10921 tggaagacgc catcagaacc ggcgcttttg tggttgcgat gtccctgtgg cataaccatc
    10981 cgaagaagac gcagatgccg tccatgaatg aagccgttaa acagattgag caggaagtgc
    11041 ttaccacctg gcccacagag gcaatttctc atgctgaaaa cgtggtgtac cggctgtccg
    11101 gtatgtatga gtttgtggtg aatgatgctc ctgaacaggc agaggacgcc gggcctgcag
    11161 agcctgtttc tgcgggaaag tgttcgacgg tgagctgagt tttgccctga aactggcgcg
    11221 tgagatgggg cgacccgact ggcgcgccat gcttgccggg atgtcatcca cggagtatgc
    11281 cgactggcac cgcttttaca gtacccatta ttttcatgat gttctgctgg atatgcactt
    11341 ttccgggctg acgtacaccg tgctcagcct gtttttcagc gatccggaga tgcatccgct
    11401 ggatttcagt ctgctgaacc ggcgcgaggc tgacgaagag cctgaagatg atgtgctgat
    11461 gcagaaagcg gcagggcttg ccggaggtgt ccgctttggc ccggacggga atgaagttat
    11521 ccccgcttcc ccggatgtgg cggacatgac ggaggatgac gtaatgctga tgacagtatc
    11581 agaagggatc gcaggaggag tccggtatgg ctgaaccggt aggcgatctg gtcgttgatt
    11641 tgagtctgga tgcggccaga tttgacgagc agatggccag agtcaggcgt catttttccg
    11701 gtacggaaag tgatgcgaaa aaaacagcgg cagtcgttga acagtcgctg agccgacagg
    11761 cgctggctgc acagaaagcg gggatttccg tcgggcagta taaagccgcc atgcgtatgc
    11821 tgcctgcaca gttcaccgac gtggccacgc agcttgcagg ggggcagaat ccctggctga
    11881 tcctgctgca acagggtggt caggttaagg actccttcgg cgggatgatc cccatgttca
    11941 gggggcttgc cggcgcgatc accctgccga tggtgggggc cacctcgctg gcggtggcga
    12001 ccggtgcgct ggcgtatgcc tggtatcagg gcaactcaac cctgtccgat ttcaacaaaa
    12061 cgctggtcct ttccggtaat caggcgggac tgacggcaga tcgtatgctg gtcctgtcca
    12121 gagccgggca ggcggcaggg ctgacgttta accagaccag cgagtcactc agcgcactgg
    12181 ttaaggcggg ggtaagcggt gaggctcaga ttgcgtccat cagccagagt gtggcgcgtt
    12241 tctcctatgc atccggcgtg gaggtggaca aggtcgctga agccttcggg aagctgacca
    12301 cagacccgac gtcggggctg acggcgatgg cacgccagtt ccataacgtg acggcggagc
    12361 agattgcgta tgttgctcag ttgcagcgtt ccggcgatga agccggggca ttgcaggcgg
    12421 cgaacgaggc cgcaacgaaa gggtttgatg accagacccg ccgcctgaaa gagaacatgg
    12481 gcacgctgga gacctgggca gacaggactg cacgtgcatt caaatccatg tgggatgcgg
    12541 tgctggatat tggtcgtcct gataccgcgc aggagatgct gattaaggca gaggctgcgt
    12601 ttaagaaagc agacgacatc tggaatctgc gcaaggatga ttatttcgtt aacgatgaag
    12661 cacgggcgcg ttactgggat gatcgtgaaa aaaaacgcct ggagcgggat gcagcccaaa
    12721 agagggtaga tcaacagcgt caacaggaca aaaatgcgca gcagcagagc gataccgaag
    12781 cgtcacggct gaaatatacc gaagaggcgc agaaggctta cgaacggctg cagacgccgc
    12841 tggagaaata taccgcccgt caggaagaac tgaacaaggc actgaaagac ggaaaaatcc
    12901 tgcaggcaga ttacaacacg ctgatggcgg cggcgaaaaa ggattatgaa gcgacgctga
    12961 aaaagccgaa acagtccggc gtgaaggtgt atgcgggcga tcgtcaggaa gacagtgctc
    13021 atgcggccct gctgacgctt caggcagaac tccggacgct ggagaagcat gccggagcga
    13081 atgagaaaat cagccagcag cgccgggatt tgtggaaggc agaaagtcag ttcgcggtac
    13141 tggaggaggc ggcacaacgt cgccagctgt ccgcacagga gaaatccctg ctggcgcata
    13201 aagacgagac gctggagtac aaacgccagc tggctgcact tggtgacaag gttacgtatc
    13261 aggagcacct gaatgcgctg gcgcagcagg cggataaatt cgcacagcag caacgggcaa
    13321 aacgggcagc cattgatgcg aaaaaccggg ggctgactga ccggcaggca gcgcgggaag
    13381 ccacggaaca gcgcctgaag gaacagtatg gcgataatcc tctggcgctg aataacgtca
    13441 tgtcagagca gaaaaagacc tgggcggctg aagaccagct tcgcgggagc tggatggcag
    13501 gcctgaagtc cggctggagt gagtgggaag agagcgccac ggacagtatg tcacaggtta
    13561 aaagtgcagc cacgcagacc tttgatggta ttgcacagaa tatggcggcg atgctgaccg
    13621 gcagtgaaca gaactggcgc agcttcaccc gctccgtgct gtccatgatg acagaaattc
    13681 tgcttaagca ggcaatggtg gggattgtcg ggagtatcgg cagcgccatt ggcggggctg
    13741 ttggtggcgg cgcatccgcg tcaggtggta cagccattca ggccgctgcg gtgaaattcc
    13801 attttgcaac cgggggattt acgggaaccg gcggcaaata tgagccagcg gggattgttc
    13861 accgtggtga atttgtcttc acgaaggagg caaccagccg gattggcgtg gggaatctct
    13921 accggctgat gcgcggctat gcggaaggtg gttatgtggg cggtgccgga agtccggcgc
    13981 agatgcggcg ggctgaaggc attaatttta atcagaacaa tcacgtggtg attcagaacg
    14041 acggtacgaa tggtctgcca ggtccacaga tgatgaaggc cgtgtatgac atggcccgca
    14101 agggtgcccg tgatgaaatt cagacacaga tgcgtgatgg tggcctgttc tccggaggtg
    14161 gacgatgaaa accttccgct ggaaagtgaa acccggtatg gatgtggctt cggccccttc
    14221 tgtaagaaag gtgcgctttg gtgatggcta ttcccagcga gcgcctgccg ggctgaatgc
    14281 caacctgaaa acgtacagcg tgacgctttc tgtcccccgt gaggaggcca tggtactgga
    14341 gtcgtttctg gaagagcacg ggggctggaa agcctttctg tggacgccgc cttatgagtg
    14401 gcggcagata aaggtgacct gcgcaaaatg gtcgtcgcgg gtcagtatgc tgcgtgttga
    14461 gttcagcgca gagtttgaac aggtggtgaa ctgatgcagg atatccggca ggaaacactg
    14521 aatgaatgca cccgtgcgga gcagtcggcc agcgtggtgc tctgggaaat cgatctgaca
    14581 gaggtcggtg gagaacgtta ttttttctgt aatgagcaga acgaaaaagg tgagccggtc
    14641 acctggcagg ggcgacagta tcagccgtat cccattcagg ggagtggttt tgaactgaat
    14701 ggcaaaggca ccagtacgcg ccccacgctg atggtttcta acctgtacgg tatggtcacc
    14761 gggatggcgg aagatctaca gagtctggtc ggcggaacgg tggtccggcg taaggtttac
    14821 gcccgttttc tggatgcggt gaacttcgtc aacggaaaca gtgacgccga tccggagcag
    14881 gaggtgatca gccgctggcg cattgagcag tgcagcgaac tgagcgcggt gagtgcctct
    14941 tttgtactgt ccacgccgac ggaaacagat ggcgctgttt ttccgggacg tatcatgctg
    15001 gccaacacct gcacctggac ctatcgcggc gatgagtgcg gttatcacgg tccggcggtc
    15061 gcggatgaat atgaccagcc aacgtccgac atcacgaagg ataaatgcag caaatgcctg
    15121 agtggctgta agtttcgcaa taacgtcggc aactttggcg gcttcctttc cattaacaaa
    15181 ctttcgcagt aaatcccatg acagagacag aatcagcgat tctggcgcac gcccggcgat
    15241 gtgctccagc ggagtcgtgc ggcttcgtgg taagaacgcc ggagggggaa agatatttcc
    15301 cctgcgtgaa tatctccggt gagccggagg cgtatttccg tatgtcgccg gaagactggc
    15361 tgcaggcaga gatgcagggt gagattgtgg cactggtcca cagccacccc ggtggtctgc
    15421 cctggctgag tgaggccgac cggcggctgc aggtgcagag tgatttgccg tggtggctgg
    15481 tctgccgggg ggcgattcac aagttccgct gtgtgccaca tcttaccggg cggcgctttg
    15541 agcacggggt gacggactgt tacacgctgt tccgggatgc ttaccatctg gcggggattg
    15601 agatgccgga ttttcatcgt gaggatgact ggtggcgtca cggtcagaat ctctatctgg
    15661 ataatctgga ggccacaggg ctgtatcagg tgccgttgtc agcggcgcag ccgggcgatg
    15721 tgctgctgtg ctgttttggt tcatcggtgc cgaatcatgc cgccatttac tgtggtgacg
    15781 gcgagctgct gcaccatatt cctgaacaac tgagcaaacg agagaggtat accgacaaat
    15841 ggcagcgacg cacacactcc ctctggcgtc accgggcatg gcacgcatct gcctttacgg
    15901 ggatttgcaa cgatttggcc gccgcatcga ccttcgtgtg aaaacgggtg ccgaagccat
    15961 ccgggcgatg gccacacagc tccctgcgtt tcgtcagaag ctgagcgacg gctggtatca
    16021 ggtgcgtatt gccgggcagg atgttagcac gtccggatta acggcacagt tacatgaggc
    16081 tctgcctgac ggcgctgtga ttcatattgt tcccagagtc gccggggcca agtcaggtgg
    16141 tgtattccag attgtcctgg gagcagccgc cattgccgga tcattcttta ccgccggagc
    16201 cacccttgca gcatgggggg ctgccattgg tgccggtggt atgaccggca ttctgttttc
    16261 tctcggtgcc agtatggtgc tcggcggtgt ggctcagatg ctggcaccga aagccagaac
    16321 tccccgcaca cagacaacgg ataacggtaa gcagaacacc tatttctcct cactggataa
    16381 catggttgcc cagggcaatg ttatgcctgt tctgtatggt gaaatgcgcg tggggtcacg
    16441 tgtggtatct caggagatca gcacggcaga cgaaggggac ggtggtcagg ttgtggtgat
    16501 tggtcgctga tgcaaaatgt tttatgtgaa atcgcctccg ggcggtttta tcgtttatgg
    16561 agcatgacga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag cgaaggacaa
    16621 cctgaagtcc acgcagctgc tgagtgtgat cgatgccatc agcgaagggc cggttgaagg
    16681 tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg acagtgaggg
    16741 gaataccaac atctccggcg tcacggtggt gttccgggca ggtgagcagg agcagtcacc
    16801 gccggaggga tttgaatcct ccggctccga gacggtgctg ggtacggaag tgaaatatga
    16861 cacgccgatc acccggacca tcacgtcggc aaacattgac cgactgcgtt ttaccttcgg
    16921 tgtacaggca ctggtggaaa ccacctcaaa gggagacagg aatccgtctg aagttcgcct
    16981 gctggttcag atacagcgta acggtggctg ggtgacggaa aaagacatca ccattaaggg
    17041 caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc cgcgcccgtt
    17101 taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc agaacaaaac
    17161 gctctggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggcact
    17221 ggtcggcgta caggtggatt cggagcagtt cggcagccag caggtgagcc gtaattatca
    17281 tcttcgcggg cgcattctgc aggtgccgtc gaactataat ccgcagacgc ggcaatacag
    17341 cggtatctgg gacggaacgt taaaaccggc atacagcaac aacatggcct ggtgtctgtg
    17401 ggatatgctg acccatccgc gctatggcat ggggaaacgt cttggtgcgg cagatgtgga
    17461 taaatgggcg ctgtatgtca tcggccagta ctgtgaccag tcggtgccgg acggctttgg
    17521 cagcacggag ccgcgcatca cctgtaatgc ctggctgacc acacagcgca aggcgtggga
    17581 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg ggcagacgct
    17641 gacgttcgtg caggaccgac agtcggataa ggtgtgggcc tataaccgca gtaatgtggt
    17701 gatgccggat gatggcgcgc cgttccgcta cagtttcagc gccctgaaag accgccataa
    17761 tgccgttgag gtgaactgga ttgacccgga taacggctgg gagacggcga cagagcttgt
    17821 ggaggatacg caggccattg tccgttacgg tcgtaacgtc acgaagatgg atgcctttgg
    17881 ctgtaccagc cgggggcagg cacaccgcgc cgggctgtgg ctgattaaaa cggaactgct
    17941 ggagacgcag accgtggatt tcagcgtggg tgctgaaggg cttcgtcatg taccgggcga
    18001 tgtcattgaa atctgcgatg atgactatgc cggtatccgc accggcgggc gcgtgctggc
    18061 ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc catcctccgg
    18121 caccacgctg ataagcctgg ttgacgggca ggggaatccg gtcagcgtgg aggtccagtc
    18181 cgtcaccgac ggcgtgaagg taaaagtgag ccgtgttcct gacggtgttg ctgaatacag
    18241 cgtgtggggg ctgaagctgc cgacgctgca ccagcgattg ttccgctgcg tgagtatccg
    18301 tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtgccgg aaaaagaagc
    18361 catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga atggtgtcac
    18421 gccgccagcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg aatatcaggt
    18481 gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agcttcctgc tccgtctgac
    18541 cgtaacagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga cggaaaccac
    18601 ttaccgcttc acacaactgg cgctggggaa ctacaggctg acagtccggg cagtaaatgc
    18661 gtgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac cggcagcgct
    18721 gtcgcggatt gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt
    18781 ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggattg cggatatcag
    18841 gcaggttgaa accacagcac gctatcttgg cacggcgctg tactggatag ccgccagtat
    18901 caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca ccgttggcaa
    18961 atcggcattc gtggaggctg ttggtcagcc gagtgatgat gcatccggtt atctggattt
    19021 tttcaaaggc gagataggga aaacccatct ggctcaggag ctgtggacgc agattgataa
    19081 cggtcagctt gcgcctgacc tggctgaaat caggacgtcc attacggatg tcagcaatga
    19141 aatcacgcag accgtcaata agaaactgga agaccagagt gcggcaattc agcagataca
    19201 gaaggttcag gttgatacaa ataataacct gaacagcatg tgggctgtga agctgcagca
    19261 gatgcaggac ggacgccttt atatcgcggg tattggtgcc ggtattgaga acacccctga
    19321 cggcatgcag agtcaggtgc tgctggcggc ggacaggatt gcgatggtta atcctgcgaa
    19381 tggcaacaca aaaccgatgt ttgttggtca gggcgatcag atattcatga acgacgtgtt
    19441 cctgaaacgc ctgacggccc ccaccattac cagcggtgga aatccatcgg cattttccct
    19501 gactccggac ggaaagctga ccgctaaaaa tgcggatatc agtggcagtg tgaatgcgaa
    19561 cgccgggacg ctcaacaatg tcacggtaaa tgaaaactgt acgattaagg gcatgctgga
    19621 ggcgactcag gtcagaggtg acttcgttaa agctgtatcc aaatcatttc cgaaacaggc
    19681 tggtacgtgg ggtaacacgg aaacaccaaa cgggacggtt acagtcacca taagcgatga
    19741 tcataacttt gaccgccaga ttattattcc gcccattatt tttaacggta tagcgtatga
    19801 cgatccgggg agcggaaata acccaggagg cacgcgatac acgggttatg gttttgaagt
    19861 tcgcaaaaac ggcgtattaa tcgcatccag agaaactaaa ggtgccattc ccggtagtta
    19921 cagtgcagtt attgatatgc ctagtggtgg tggtagcgtc actctggagt ttaagatttt
    19981 ccagaaaggc aatcaggggg caggcaatat caccgactgt acggtgattg tgaccaaaaa
    20041 agcggcttcc ggcatcagta ttcgttgaaa tatttataac cccaataaat ggcgtcagga
    20101 atgacgcctt ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca
    20161 ttttgtccgc agccatttgc ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc
    20221 agtccacgct gagcgcgggg tatcttcatg cccggaccaa cgttcccggc agtgatgatc
    20281 tgaacgggat taacgtgaaa taccgttatg agtttacgga tacgctgggg ctggtgacgt
    20341 cattcagcta tgcaggagac aagaatcgcc agctgacccg ttacagcgat acccgctggc
    20401 atgaagattc cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga
    20461 atgaatggtt cagcgcgtat gcgatggcgg gtgtggctta cagccgtgtg tcgacttttt
    20521 ccggggatta tatccgcgta actgacaata aggggaaaac gcacgatgtg ctgaccggaa
    20581 gtgatgacgg tcgccacagc aacacgtctc tggcgcactg acggtttcat cgtgggtgtc
    20641 ggttataaat tctgattagc caggtaacac agtgttatga cagcccgccg gttcaggcgg
    20701 gcttttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag acggcacagg
    20761 aaaaccggta cagaactgca caatccagct gaaagcaaaa cgtaacagca ccacggtggt
    20821 ggtgaacacg ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga
    20881 gtacggtcag tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac
    20941 catcaccgtg tatgaagatt cccgacccgg tacgctgaat gattttctcg gtgccatgac
    21001 ggaggatgat gcccgtccgg aggcactgcg ccgttttgag ctgatggtgg aagaggtggc
    21061 gcgtaacgcg tccgcggtgg cacagaacac ggcagccgcg aagaaatcag ccagcgatgc
    21121 cagcacatca gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc
    21181 agccagcacg tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg
    21241 aacggcatca acaaaggcca ctgaagcctc aaaaagtgct gccgctgcag agtcctcaaa
    21301 aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtta gaaacgaatg cggcagcgtc
    21361 acaacaatca gcagccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac
    21421 ctcagcccgg gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc
    21481 atcaagcgcc agtagcgcag cttcctcggc gacggcggct gcaaattctg cgaaggcggc
    21541 caaaacgtcc gagacgaacg ccaggtcttc tgaaacggca gcgggacaga gtgcctcagc
    21601 tgcggcaggc tcaaaaacag cggctgcgtc gtctgccagc gccgcgtcaa caagtgccgg
    21661 gcaggcctca gccagtgcca ccgccgccgg aaaatcggca gaaagcgccg catcatccgc
    21721 ttcaacagcc acaacgaagg ctggcgaagc cactgagcag gccacggcag cagcgaggtc
    21781 agcttccgca gcgaagacat ccgaaacgaa cgcgaaagcg tcggaaaccc gtgcagaatc
    21841 ctcaaaaacg gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catcggcgtc
    21901 tgcttcaaaa gatgaggcga ccagacaggc gtcagcagcg aagggcagcg ccacgacggc
    21961 atccacgaag gcgacagagg ctgctggtag tgcgacggca gcagctcaga gcaaaagtac
    22021 ggcggaatct gcagcaacgc gcgctgagac agcggcaaaa cgggcagagg atattgcatc
    22081 cgccgtggcg cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagtagtgc
    22141 gactaacagc acttccgagt cactggcggc aacaccaaaa gccgttaagg ccgcgtatga
    22201 cctggctaac gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggataatcca
    22261 gctaagcagc gcgaccaaca gcacgtctga aacgctggcg gcaacgccaa aggcagtaaa
    22321 agcagccaat gacaatgctg agaaacgtct gcagaaagat cagaacggtg cggatatccc
    22381 tggcaaagac acctttacga aaaatattgg tgcctgccgt gccttcggtg ggtcagtaag
    22441 cacaacaaca ggaaactgga cgactgcaca gtttatcgag tggctggatt ctcagggagc
    22501 atttaaccat ccatactgga tgtgcaaggg ttcctggtct tatggcaata ataaaatcat
    22561 tactgatact ggctgcggta atattcatct cgccggagct gtcattgaag taatggggat
    22621 aaagtcagcg atgacgatcc gcattaccac tccgaccaca tccagcggtg gcggaacaac
    22681 caacgcccag tttacctata ttaatcacgg aacagattat tcacctggct ggcgaaggga
    22741 ctataactcc agaaataagc caacggcatc agagatcggg gcgctaccgt caggtggaac
    22801 agcagtatca tcagttaatc tggcttcaaa aggtcgggta gccgcgctga cagacaatac
    22861 gcagggggca gcaggtcttg agttatacga ggtgtataac aacggatatc caacagcgta
    22921 tggaaatatc attcacctga aagggatgac agccgttggc gaaggtgagt tactcatcgg
    22981 ctggagtggt acaagcggtg ctcatgctcc ggcatttatt cgttcacgac gggatacgac
    23041 cgacgcaaac tggtcgccgt gggcgcagct ttacacctcg gctcatcctc ctgcagagtt
    23101 ttatccagtc ggtgcaccaa tcccgtggcc atcagatacc gttccgtctg gttatgccct
    23161 gatgcagggg cagacttttg acaaatctgc ttacccgaaa cttgcagccg cttatccgtc
    23221 aggcgtgatc cctgatatgc gtggctggac gattaagggc aagcccgcca gtggtcgtgc
    23281 cgtattgtct caggaacagg atggcattaa atcgcacacc cacagcgcca gcgcatccag
    23341 tacggatttg gggacgaaaa ccacatcgtc gtttgattac ggcactaaat ccacgaataa
    23401 caccggggcg catacgcaca gtctgagcgg ctctacaagt tcagctgggg cccaccaaca
    23461 tagtcagact gggcccagaa cgaatagcgg gagtcaacct actggaatgt ttccggcagg
    23521 gagtactcaa gttagcggga ctaatcaggt tggtatcagt ggctcattaa ccagtggcac
    23581 ctcccaatgg gtaggtaaat catcttcaga aggaaaccat acacactcac tgtccggcac
    23641 agcagcatct gcgggtgcac acgcgcatac tgtcggtatt ggtgctcata cgcactctgt
    23701 tgcgattggt tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
    23761 cgtcaaaaac atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt
    23821 gaacaagcac ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt
    23881 gaaggtgacg catatattcc gcctcataca ggtctgcctg caaacagtac cgatattgca
    23941 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgagtc atcgtggcat
    24001 ctcgttgaag atcatcgggg taaaacggtt tatgacgtgg cttccggcga cgcgttattt
    24061 atttctgaac tcggctcatt accggaaaat gtcacctggt tatccccgga aggggagttt
    24121 cagaagtgga acggcacagc ctgggtgaag gatacggaag cagaaaaact gttccggatc
    24181 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
    24241 cttcaggatg ctgtggatct ggagatcgca acggagaaag aaaccttgtt gctggaagct
    24301 tggaaaaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc ggatattgaa
    24361 tggccagtag cacctatagg gtaaattcgt aatgattacc taaatacgtt attcttttgt
    24421 taaaaaagtg attttgctat aggtagggag tatgttcttg gattaagaat aaaaaatcgc
    24481 catgaaagag cttcatggcg agtacataaa cgtacaaaat gtgatga
//
LOCUS       DAZSBF010000043        24289 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZSBF010000043 DAZSBF010000000
VERSION     DAZSBF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.043"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            176..313
                     /locus_tag="ACHXWD_001351"
                     /pseudo
     CDS             176..313
                     /locus_tag="ACHXWD_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(421..1656)
                     /locus_tag="ACHXWD_001352"
     CDS             complement(421..1656)
                     /locus_tag="ACHXWD_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR7620316.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1803..1879)
                     /locus_tag="ACHXWD_001353"
     tRNA            complement(1803..1879)
                     /locus_tag="ACHXWD_001353"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1843..1845),aa:Pro,seq:tgg)
     gene            complement(1922..2008)
                     /locus_tag="ACHXWD_001354"
     tRNA            complement(1922..2008)
                     /locus_tag="ACHXWD_001354"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1972..1974),aa:Leu,seq:cag)
     gene            complement(2029..2104)
                     /locus_tag="ACHXWD_001355"
     tRNA            complement(2029..2104)
                     /locus_tag="ACHXWD_001355"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2069..2071),aa:His,seq:gtg)
     gene            complement(2163..2239)
                     /locus_tag="ACHXWD_001356"
     tRNA            complement(2163..2239)
                     /locus_tag="ACHXWD_001356"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2203..2205),aa:Arg,seq:ccg)
     gene            complement(2340..3725)
                     /locus_tag="ACHXWD_001357"
     CDS             complement(2340..3725)
                     /locus_tag="ACHXWD_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7620317.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3916..4656)
                     /gene="wecG"
                     /locus_tag="ACHXWD_001358"
     CDS             complement(3916..4656)
                     /gene="wecG"
                     /locus_tag="ACHXWD_001358"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR7620318.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4659..6011)
                     /gene="wzyE"
                     /locus_tag="ACHXWD_001359"
     CDS             complement(4659..6011)
                     /gene="wzyE"
                     /locus_tag="ACHXWD_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR7620319.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6008..7087)
                     /gene="rffT"
                     /locus_tag="ACHXWD_001360"
                     /gene_synonym="wecF"
     CDS             complement(6008..7087)
                     /gene="rffT"
                     /locus_tag="ACHXWD_001360"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR7620320.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7084..8334)
                     /gene="wzxE"
                     /locus_tag="ACHXWD_001361"
     CDS             complement(7084..8334)
                     /gene="wzxE"
                     /locus_tag="ACHXWD_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR7620321.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8336..9466)
                     /gene="rffA"
                     /locus_tag="ACHXWD_001362"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8336..9466)
                     /gene="rffA"
                     /locus_tag="ACHXWD_001362"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR7620322.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9471..10145)
                     /gene="rffC"
                     /locus_tag="ACHXWD_001363"
                     /gene_synonym="wecD"
     CDS             complement(9471..10145)
                     /gene="rffC"
                     /locus_tag="ACHXWD_001363"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR7620323.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10123..11004)
                     /gene="rfbA"
                     /locus_tag="ACHXWD_001364"
     CDS             complement(10123..11004)
                     /gene="rfbA"
                     /locus_tag="ACHXWD_001364"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR7620324.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11023..12090)
                     /gene="rffG"
                     /locus_tag="ACHXWD_001365"
     CDS             complement(11023..12090)
                     /gene="rffG"
                     /locus_tag="ACHXWD_001365"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR7620325.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12090..13352)
                     /gene="wecC"
                     /locus_tag="ACHXWD_001366"
     CDS             complement(12090..13352)
                     /gene="wecC"
                     /locus_tag="ACHXWD_001366"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR7620326.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13349..14479)
                     /gene="wecB"
                     /locus_tag="ACHXWD_001367"
     CDS             complement(13349..14479)
                     /gene="wecB"
                     /locus_tag="ACHXWD_001367"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR7620327.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14535..15581)
                     /gene="wzzE"
                     /locus_tag="ACHXWD_001368"
     CDS             complement(14535..15581)
                     /gene="wzzE"
                     /locus_tag="ACHXWD_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR7620328.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15593..16696)
                     /gene="wecA"
                     /locus_tag="ACHXWD_001369"
     CDS             complement(15593..16696)
                     /gene="wecA"
                     /locus_tag="ACHXWD_001369"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR7620329.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16936..18195)
                     /gene="rho"
                     /locus_tag="ACHXWD_001370"
     CDS             complement(16936..18195)
                     /gene="rho"
                     /locus_tag="ACHXWD_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR7620330.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18280..18381)
                     /gene="rhoL"
                     /locus_tag="ACHXWD_001371"
     CDS             complement(18280..18381)
                     /gene="rhoL"
                     /locus_tag="ACHXWD_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR7620331.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18522..18851)
                     /gene="trxA"
                     /locus_tag="ACHXWD_001372"
     CDS             complement(18522..18851)
                     /gene="trxA"
                     /locus_tag="ACHXWD_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR7620332.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18982..20247
                     /gene="rhlB"
                     /locus_tag="ACHXWD_001373"
     CDS             18982..20247
                     /gene="rhlB"
                     /locus_tag="ACHXWD_001373"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR7620333.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20381..21865
                     /gene="gppA"
                     /locus_tag="ACHXWD_001374"
     CDS             20381..21865
                     /gene="gppA"
                     /locus_tag="ACHXWD_001374"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR7620334.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21912..23933)
                     /gene="rep"
                     /locus_tag="ACHXWD_001375"
     CDS             complement(21912..23933)
                     /gene="rep"
                     /locus_tag="ACHXWD_001375"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR7620335.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24150..>24289
                     /locus_tag="ACHXWD_001376"
     CDS             24150..>24289
                     /locus_tag="ACHXWD_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620336.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6102 a         6653 c         6004 g         5530 t
ORIGIN      
        1 gcgccagagg taggattgaa aacgctctcc tgattttcca attcattttc tggataaata
       61 aataatttat ttttgtcact attatttatg taatcatcct gtcagggaga gggatctcaa
      121 ttatcaatgc ttaattacgt catcattttg atgacatgaa accggagcat tttgcatgga
      181 attttcgttt tcacccaaac ttcttgttgt tgctgtcgcc gccgctcttc ctctcatggc
      241 cagcgcagca gataccccgt caactgccac cgcacgcaaa ggctttgccg gatacgatca
      301 cccaaaccag tatccacaga acgctgcaat ttattgaatt tgcacgatca tgtaggccgg
      361 ataaggcgtt tacgccgcat ccggcaatca accgcaggcg gccacaaaat ggccgctttt
      421 ttacttattc accaccagca aatgtgcctg cataatgtca ctggccgggc gaccgtgcgc
      481 cagcaaatca gccattgctt taagatatgg cggtagatgg cggaaataac gctgataccc
      541 ggcacacaaa taattcagcc ccggtttgcc gctggcatcg agcatgaagc ggtgtttcgg
      601 gcagcctccc cagcacgctt ttaacacgtt acaactgcga cactgcgccg gtaattgctt
      661 aaatttatct tcaccaaaca cctgctgttg cggggaatcg atcatttctg caattgtttg
      721 ctgatgcata ttccccagcc gatattgcgg ataaacatag tgatcgcagg cgtaaacgtc
      781 gccgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc aaaccgcacc
      841 cggcgcaccg acaaaattgg caaacgccca ttcgatattc atcacgaaaa tcttgccgac
      901 gtcgcgtttg atccagtggt cgaatatcgc caccagaaat tcaccaaatt catcggggcg
      961 caccgaccat tccgtcagtt ccccctgaat atcaccaggc gcatgcaatt tcagtccatc
     1021 gcgggcagct gtttcatcag ccagacgctc gaccaccgga ataaactgga tgaattcgac
     1081 tcccgcatcg cacaaaaaat cataaacctg caacggttgc tgcgcgctgg tgcggttgac
     1141 gcagaccagc acgttatagt cgacatgatg tttttgcagc agcgtcaggg cacgcatcac
     1201 cagcttatgc gttggtctgc cacctttggt cacgcgatat tgattgtgga tctcagccgg
     1261 accgtccagc gataacccaa caagaaaatg attttctgcc agaaatgcac accatttatc
     1321 atcgagcagc acgccgttag tctggaagct gttacttatc ttcctgccag caccgtattt
     1381 cgcctgtaac tttacggcac ggcggtaaaa atccagcccg agtaacgttg gttcgccgcc
     1441 ctgccaggta aaagccactt cgttttgcgg ttcgctggca gcgatatagt gacggacata
     1501 cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt tcgcggtaaa gggattgttt
     1561 ttcgagataa aaacagtagt cacagttcag attacaatcg gaaccactcg gtttcgccat
     1621 cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc actccttgcc tgaagtcatc
     1681 cttcaatacg ttaagggcga aatcataccg tggggcaatg agttattacg ggcgtttatt
     1741 gcgaagggag ggtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc ggggttcaaa
     1801 attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca gttgcgctac
     1861 caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt ctttaaagtc
     1921 gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta
     1981 gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg gggtggctaa
     2041 tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac tgagctatag
     2101 ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc gccgggacta
     2161 aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc agcgctctat
     2221 ccagctgagc tacgggcgct tagcgccgtt gcggggggat aatacggact tcccacccct
     2281 ctgtctagtc cctttttaaa taaattgcgc gtttggttac ggtttgtgcg ttttgctgct
     2341 tattcctcca gtttatgcgc tttcccgtgg cgattaaggc caaaaacttt ataaatcgcc
     2401 gtcaccgcca gcataaagat gatgccaaca aacagcgaca tacgcgtatc ttcattaaag
     2461 tacatgccga tcaaaacgca aatcaggaat gccattgtta cgtagttggc ccacgggaac
     2521 aggattgagc ggaacggatg gctggcaatc gccgctttat gtgcacgccg aaaacgcagc
     2581 tggcttatca atatcacaaa ccatggcacc atccccggaa gcacgctggc actgtagacg
     2641 tagacaaaca cacgctgcgg attgggaatg atgtagttca ggcatgagcc aattagcaga
     2701 atagcaatag atactgccac acccgcaact ggtacgccgt gacgggaaac tttcgccatc
     2761 gccgccggta actgacggtt tttcgccagt gcgtagagca tacgtccgca actgtacatg
     2821 ccgctgttac agccagagag cgcagccgtc agcaccacaa agttgataat gcccgccgct
     2881 gcggtaatac cgattttggc aaaagtcagt acgaacgggc tgccgttgct gccaatttca
     2941 ttccacggga agatggtgac gataacgaaa atcgcgccta cgtagaaaat caggatccgc
     3001 cacagcactt tgcctacagc actgcgcagc gtcacctgcg gattcttcgc ttcaccggca
     3061 gtaatgccaa tcagctccac gccctggtag gacgccacca caatacacag cgcggtcagg
     3121 aaccctttcc agccacccgc aaagaaaccg ccatgctctg tgagattgct aaaaccaatc
     3181 gactgcccgc cattgccaaa gccaaagaaa atcacgccca ggccaatgac aatcatcacg
     3241 ataatcgtgg tgactttgat catcgcgaac cagaactcga tttcgccgta caaccgcacc
     3301 gccgccagat tcgccaacgc caccagcgcc accgcgatca atgcaggtat ccactgcgcc
     3361 atctccggga accagaactg gacataaacg ccaatggcgg tgatttctga gatccccacc
     3421 gccatccaca taaaccagta agaccaggcg gtgagataac caaaaaacgg gctcatataa
     3481 cgatgcgcat aaacggcgaa cgaaccggta acaggttcaa ggaacaacat ttcgcccatt
     3541 gaacgcatga tgaaaaagac gaacagcccg gcgatgatat aggccaacaa tacggatggc
     3601 ccggcccatt tcagggtact ggcggccccc ataaacaggc cgacgccaat agtgcccccc
     3661 agggcgatga gttcgatatg tcgagcttcc agcccacgct gtagctctgg tttgttatct
     3721 gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt taccggttat cgttatgggt
     3781 acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat ggcaaataaa gcattaaaaa
     3841 tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg agtgaccact ccgtcgcttt
     3901 acaaagagag gaaaatcaga ggttgccggt gtagtgccag cgtaaataac gcagcaaacg
     3961 aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg tagagccact ccagtcccag
     4021 cgtttgccag attttcggtg cgcgttttac gtgaccggtg aaaacatcgt aagtcccgcc
     4081 aacgcccata tacagcgcat ctggatgcac cagacggcag tcgcgcatga agatctcctg
     4141 ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg ctggcatgaa tgcgttcaaa
     4201 cagcgcctga cgctgctcgg gtttaaaata accatcctga ctgccaacga tattcacatt
     4261 ccattggttg cgcagtttag cttcagtttg cgccagcact tcaggtttac cgcccacaag
     4321 aaataccggc gtcccttctt tgcctgcgcg cgccatcagc tcttcccaga gatcggcacc
     4381 ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt actgaacgta caacgctgat
     4441 gccatccgca tatttaaatt cggcagcgtt aattaactcc ctgacctcgg cgttatcttc
     4501 aatggtcagc attttttcag cattaatggc aaccagcgtt ccctgcttaa gctgcccgtc
     4561 agcaaacaga taatcgaggg cgtgctgcat atcacgccaa ccaatcaact gtaagccacg
     4621 cagcgtatag gtcggtgccg tggtgttgtt attcattgtt atccttcaac ctgcgtccgg
     4681 agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt caaaaagcca gtacagcagt
     4741 tttgcgatca tcagacatgc gccgaagacc acgataaaaa agactacgcg tgagacaaac
     4801 gaatccagcc cttcacgcgc cagcacgatc atattgaaga tcgccccaaa gcagaaactg
     4861 tgcaatatcg cagccttata gcgattaggc tcgcggttgc ccagctcata cagccagtcg
     4921 aaccatttga tgatcagacc aaccacgatc gccccgagcg ggatgaacaa cgcgccgccc
     4981 atcaccacca gtgagcctat aagcgtaggc gagatcgcca gtccggagtg gttattcagc
     5041 acttcccagg taaagtagtt ggctgagttc agcaccatac tcgggcgacc cggccacagc
     5101 caggaaggga taaagacata gaaatcgcgg acaattggag ccaggccctg gaagtcgatg
     5161 ttgtcgtagt tctgcaacag caacgccaga ttctcccacg gggagaaggt gtcgcgagtg
     5221 agatagagaa acgtatagaa cgcttcatcg ccgctcacat tcattccata gcgttttagc
     5281 gccagccaga acatgccaac aatccccagc acgcccgccg ccgccagcat ccacaacgaa
     5341 atccagccgc gaataatgcc aataaacagg aagatagcga atgcgatgat gatattggcg
     5401 cgagtgccgc cgacaatcat ataagtcagc aagccaaagg cgaccgtgct gacgaggaaa
     5461 aacagccacg ctttgctgtc ctggcgcaga aagtagacca ccagcatcgc cgggatgaaa
     5521 aagtaaaaga agcgttttaa cgccacgccg gagacttcac tggagaagat ctgactgtag
     5581 gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga agatgccgac gcttaccagc
     5641 gcgataccca tcaggatcac ccacgtaaga ttggtctcca cgcggttcat ggtaaacagc
     5701 ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg ttttgtaggt gacatagtaa
     5761 accgcgtaga agcagcccgc agaaagcaac gcctgcaaca agatttctgg cggcgcgaca
     5821 ccaacatcaa agcgaaatac cagcacgctg gtcagcggga agccgaagaa aaaggtgagc
     5881 aaaaacagca atgaaaagaa gacattgaag ttaaagcgca cacggcgaaa ctcaaaccag
     5941 gtcagcgtgg caataaacag cgtgcagagc agccagacca caaacaggcc actgaattgc
     6001 agcagactca tgcgacctcc ccggcggcaa tagccaacgc ccgctgccag ccttgtagat
     6061 agttagggct aaagaaggca atggtgtttt tatccaccga cgccaactgg cgctgcgctt
     6121 cacgcacaat atcctcgttg agatcgtcgg tagtaaacag caccggcaaa tgctgttccg
     6181 tcatatcctg ccagaacgga ttttcccggt taagcacaca aggaatgccc gcctgaatca
     6241 gtaagcaaag cgtaccaatg ccctgctggc gggcaaaaat aaagtaacca agatcgcact
     6301 gacgaagtag cgccagatag gcgtcaaatt ccagtttttc gctcaaaatt tgcagatttt
     6361 cttcgctgaa taactccagc cccgcctgac gaacttcctc aatgtacgct tcgttattag
     6421 gcggatatcc catcggcacc accactttta ccgtatcgcc aaattgctga tgaacggcgc
     6481 gcaaggcagc aatatggtca ttgctgcggt caccggagtt ccccaccaga atggtcattt
     6541 tcccttcacg ttgccgatca ttcgccatcg tattgagaga agggtccatc cgcgtcggga
     6601 agtacagcag ttcgccccgc acctttggat gcgttttggc aaaaaagctc aaatcaccgc
     6661 gggtggcaaa tacacagccg actcgctttt gcgccaggcg acgtagcggg taaaaaagct
     6721 tatatctcaa gccactggaa agctcataca ggtctgcccc ccagatatgc cagaaaaact
     6781 ggctgggctt aatgccacca ctcagcagag ccagccacag tgtgggattg aactgaccgt
     6841 ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc gatgaccgct tccgccagcg
     6901 attttttccc agggaaaaat tgcacagaaa gcgccggaca gctatcactt aagccgtcgt
     6961 ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt cgcggccagc gcgtcattga
     7021 aaaaccgcaa aacggttcgg ttatggtgag ggatatccga tcccagtacg tgaatcagta
     7081 cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa caaagagaaa aatagacgat
     7141 ataagttgcc atatatgcct gcgccgcgcc cagtgcacca tgcgcaggga ttagccagtg
     7201 ggcaaatacc atcaataaag tgaactggct gacttccgcc agaatataaa accgcagtga
     7261 cgctttggcg atcaccagat aaccaaagac ataagcgccc acttttaaca catcacccac
     7321 taactgccag gcaaagagat cgcgcatagc ggtaaattta ttcgacaaca gcaaccagat
     7381 agcaaaatca cgcagcaacc agacggtaaa actcgccgcc gccactgccg gtaagacgaa
     7441 tttcagcgat ttaaccactt cccgggtgat atcgcgcttt tccgttagcc gcgacaacgt
     7501 gggcagcaaa tatacgctga acgatgccgt aataaattgc aggtaggcat cggaaatact
     7561 gctcacccct tgccagatcc ccacttcatc ccagctatac tgcgccgcca gcagtttacg
     7621 catcatgatg taagcaacag gcaaggtcac cgacgtaatc aacgccatga gcgtaaattt
     7681 gctcaactgc cctgccagac cgttatccca gctgggtttc agatagctta acgggatgac
     7741 accacgtttg atcaacatga tggcggcagg aatcaccacc agcgcgggaa tcagcgccag
     7801 acccagcaac gccccttcat aaccgcccaa acggtaactg acgtagtacg cgagaacgcc
     7861 aatcaagctg ccgacaatca gcgataacgc attacctgcg gcatcgcgaa agcctttcat
     7921 cagcgccagt aacaggttgc cccaggcgat ccccatttgc accagcgcca ctaaacgcac
     7981 cagcccctga tagtcggtat taccaaacag tccctggctg attggcgcag ctgccagcac
     8041 aaaaaccagc gccatcagcg tagagaaacc aagtaccatc gctgatgaag tgccgaccac
     8101 gcggcgcagc tgttgcggat tatcatggta ctgggcaacg tatttggtta caccgttaaa
     8161 gatgccagct ccggcaagca cgccgagcac ggtaatcaac tggcggaaat ttgccgccag
     8221 cccaagcccc gccggaccaa atgacacagc cagcaactta ccgaccagta acccggcacc
     8281 aatcttgacc agtgtactgg ccgccgtcca caaggacgct tttgccaacg acatatcagg
     8341 agaagtagtt caacaaagtc gcaattaccg tacgctgatt gacgggcgac aggttgtaga
     8401 acagcggcag gcgcagcagg cgctcgctct ctttggtggt gtagcgatct tcaccgtgga
     8461 actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg aatgtaatgg aacaccgcca
     8521 tgatttccgc ttctttcaga aagttaatca acgcgctccg gtcatcaata tcccgcagtt
     8581 taatgtagaa catatgcgcg ttctgcacgc agccatcggg aatcgacggc agctcgatac
     8641 gcccggcttt cgccagaggc gctaacgcat cgtagtagtt ttgccacagc gccagacgtt
     8701 gctggttgat acgatccgct gcttccagtt gcgcccacag gtacgcagct tgcagatcgg
     8761 acatcaaata gctggagcca atatcgcgcc aggtatattt atcgacctga ccacggaaga
     8821 actggctgcg gttagtgccc ttttcacgga tgatctcggc tcgttcgatt aacgctttat
     8881 cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata gtttttggtt tcatggaagc
     8941 taaagcagcc aatatgacca atggttccca gtgcacgccc tttgtaagtg gacatcacgc
     9001 cctgagcggc atcttctacc acaaacagat tatgcttttt cgccaacgcc ataatggtgt
     9061 ccatttcgca ggccacaccc gcgtaatgga ccggcacgat aacgcgcgtt ttgtcggtga
     9121 tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc cgggcgaaca tccacaaaaa
     9181 cgatttttgc gccacgcagc acaaaggcat tggcggtgga gacaaaggtg tagctcggca
     9241 tgatcacttc atcgccaggc tggatatcga gcagcagcgc cgccatctcc agcgaagcgg
     9301 tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg ttgctccagc cactgctggc
     9361 agcgacgggt aaaaccgcca tcgccacaca gtttgccgct acccattgcc gactgcatat
     9421 agtcgagttc ggttcccacc accggcggtg cgttaaatgg aatcatgtga tcacctgtat
     9481 aaccagtacg cggtgctttc tacattcgca ccactttgta tgtatcgttt aagcgcggcg
     9541 gtgttgccca tttgggtcgc cacccgcaaa gttgttttac cgcgagcata cgcccagttt
     9601 agcgccgttt gcatcagctc agcacctgca ccgcgtccag ccagcaggcc aattcgcgca
     9661 tctgtcgcat tgagttcccg taaagagaca taaccgcgaa taccgccgga cgccgcacgt
     9721 aaaatcagac attgatgatc aaaggtgccg cgcacggcat tttcaatcca ctgtgcataa
     9781 aagcgtccgc tggcgtcagg cgcataccac ggcgcacgaa aacggctttg cgcaaatgcg
     9841 gcgctggcta actgacgtaa tgcgggaata tcggtctctt gtgccactac agcaccacta
     9901 tcactgacat tgttcacggg tagtgccaaa tcaacttcac cttctaccag ggagaatccc
     9961 agctgttgca gggcatccag ttcacccgta tttgatgccg caattttggc ctgcacccgt
    10021 gaccacggcg ctaacacgtc tggcgtcagg agctgtgctt cagacgtaat gcgcacgatg
    10081 gcgctgttaa caccaaagaa ggcgttttcc caggttagtg gctcaatact ggcgcggacg
    10141 ggcacgaagt aactccagca gatattggcc gtagccagtt ttcgctaatg aactggcagc
    10201 acgcttcaca ccctcgtcat cgagccagcc gttacgccag gcgatctctt ccaggcaggc
    10261 aatcttaaag ccctggcgtt tttctactgt ctgcacaaag gtactggctt caatcaggct
    10321 gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg agcagttcaa cggtcaggtt
    10381 gcccgcctcg aggtacatct ggttgatgga ggtaatctcc agttcaccac gctccgacgg
    10441 cttcacctgc tttgcgtact ccacgacttt actgtcgtag aaataaagcc cggtcaccgc
    10501 ccagtttgac ttcggctgtt ttggcttttc ttccagcgag atagcgcgga aattgtcgtc
    10561 aaactccacc acgccaaagc gttccgggtc catcacctga tagccaaata ccgtcgcccc
    10621 ttccgtgcgc gccgcaacat gacgcagctt cggactgaag ccctgaccga agaagatgtt
    10681 atcgcccagc accagacaag aaggttcacc gttgaggaag gtttcaccga tgataaaggc
    10741 ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc agctggatac cgaactcact
    10801 accatcgccc agcaggcgct ggaaataacc tttatcttcc ggcgtagtga tgatgagaat
    10861 ttcgcggata tcggccaaca tcagcaccga cagcggatag taaatcattg gcttatcgta
    10921 aatcggcaac agttgcttcg atacgccgcg cgtaatcgga tgcaatcggg tgccggaacc
    10981 gcccgccagg ataatacctt tcatgtttgc ctccgctgaa aattagcctt tcagacctaa
    11041 acgctcgccc tgatagctgc cgtcctgcac ctgcttccac cagctttcat tagccagata
    11101 ccactgcacc gttttacgca ttccactttc aaaggtttcc tgtggcagcc agccaagttc
    11161 acgggcaatt ttcgaagcat caatggcata acgcagatca tgccccggac ggtcagcgac
    11221 aaaggtgatc aagtcacgat agtgcaccac gccgtgcggc ttgttcggag ccagttcttc
    11281 cagcagctcg caaatggttt ccacaacatc gagattctta cgctcgttgt gaccaccaat
    11341 attataggtt tcaccgactt tcccggtggt cgccacgcaa tacagcgcgc gggcgtgatc
    11401 ttccacatac agccagtcac ggatttgctg cccgttgcca tataccggca gcggtttacc
    11461 cgccagcgcg ttgaggatca tcagcgggat cagtttttcc ggaaagtggt aaggaccgta
    11521 gttattcgag cagttggtga taagcgtcgg cagaccgtag gtccgcagcc aggcgcgcac
    11581 cagatggtcg ctgctggctt ttgacgcgga ataagggctg ctcggcgcat acggcgtggt
    11641 ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat acttcgtcgg tggagatatg
    11701 atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta ttccagtaag cccgcgccgc
    11761 ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata aacgctgccg ggccgtcaat
    11821 agaacggtca acatggcttt cggctgccag atgcatgaca cagtctggct gatgctcagt
    11881 gaatacgcgt gccagttctg cccgatcgca gatatcaact ttctcaaagg caaagcgctc
    11941 gctttgcgcg accggtgcca gcgacatcag gtttccggcg taggtcagct tatcgaccac
    12001 taccaccgcg tcgctcgttt cgttgatgat ataacgcacc agcgccgagc caataaaccc
    12061 ggcaccacct gttatcagaa tttttctcat cagcgccaga ctcctttggc atcgacgaca
    12121 tactgctgat ggacattgtc gccattgata actttaaact gactatgatc gaccagcatc
    12181 accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg ccagagtaca aagcccggtc
    12241 agttttttcg gcaactggtg gatgttaggc tcaacaacca gagtttcgcc gctatgccac
    12301 tgggcgatca gttcagcgat ttccatcgcc gggctttcgc gcaggtcatc aatattcggt
    12361 ttaaacgcca gaccaaagca ggcgattttc agttcactgg cgcgtttatc ggtagccgcc
    12421 aggcaatcag ccaccgccgc tttcacctga tcgataaccc agaacggttt gtgatcgttc
    12481 acttcgcgcg cggtacggat aagccgcgcc tgctgggggt tctgtgccac gataaaccac
    12541 ggatcaacag caatgcagtg accgcccacg ccagggccag gctgaagaat attaacgcga
    12601 gggtgacgat tcgccaggcg aatcagttcc cagacgttaa tcccctgatc ggcacaaatc
    12661 agcgacaatt cattagcaaa agcgatattc acatcgcgga agctgttttc ggtgagctta
    12721 cacatttccg ccgtccgcga gttagtgacg acacactcac cttcgaggaa aattttgtac
    12781 agttcgctgg cgcgggtcga acaaaccggc gtcataccac caatcacgcg atcgttttta
    12841 atcagctcga ccataacctg gcccggtaac acgcgttccg ggcagtaagc aatgttgacg
    12901 tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg gacgcatctc tgctaaccat
    12961 tctgccatct tctcggttga ccccaccggc gaggtggatt caaggatcac cagcgcgcct
    13021 tttttcagca ctggcgcaat ggagcgagca gccgattcaa cgtaggtcat atctggctca
    13081 tgatcgcctt taaacggcgt gggtacagca atcagccagg catccgcttc aactggcgtc
    13141 gtgctcgctc gtaaaaaacc gccttctacg gcagttttta ctacgctcgc caaatcaggc
    13201 tcgacgatat ggatttcgcc acgattgatg gtatcaaccg cgtgttggtt gatatcgaca
    13261 ccaatcacct gtttttgccg tgaggcaaac gctgctgctg taggcagccc gatgtaaccc
    13321 agtccgataa cagaaatggt cgcaaaactc atagtgatat ccgattattt tttaacgctt
    13381 ccagaatgcg agagcatgcc tgaccatcac catacgggtt atgggcgcgg ctcatagttt
    13441 gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc gacaattcgc tgcttatccg
    13501 tgcctaccag acgcaccgta cccgccgtca ccgcttccgg acgctcagtg gtatcgcgca
    13561 tcaccagcac cggtttcccc agcgaaggcg cttcttcctg aatgccgcct gagtcggtca
    13621 aaatcagcca ggcgtggttc atcagccaga caaacggtaa atactcctgg ggatcgatca
    13681 gaatgacatt tttcacatgc cccagaatgc gattgaccgg ctctctgacg ttcgggttga
    13741 gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt ggcaatgtct gccaacgcgt
    13801 ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct gtgaccggtc accagaatca
    13861 tctttttatc ggggtcgata aacgggtaat ttgccgccag ttctgaacgc agcgtatcgc
    13921 tgctcatcac ctggtcacgc acccataaca gtgcatcaat aactgtatta ccagtaatga
    13981 agattcggct atccgcaacg ttttcacgca gcaagttttg ccgggaagtt tcggttggag
    14041 agaagtgata catcgccaga tgcccggtca atgtacggtt agcctcttcc ggccacggcg
    14101 aataaagatc gcccgtgcgc agaccagcct caacgtgacc aacaggaata cgctgataaa
    14161 acgccgccag gctggttgcc agcgtcgtcg ttgtgtcgcc gtgaaccagc acgacgtctg
    14221 gtttgaactc ggcaagaata ggttttagcc cttccagaat ccgacaggtt atctctgtca
    14281 ggccctgtcc tggctgcatt atgttgagat cgtagtcagg tacaatggaa aagagtttca
    14341 gcacctgatc gagcatctcc cgatgctgcg cagtgacgca aactttagcc tcaaaaaaag
    14401 gatcttttgc caacgcatgc accaacggcg ccatcttgat ggcttccggg cgcgtaccaa
    14461 atacagtcag tactttcaca tcgattctct tcgaataagc ggcgagcgcc tttgcgctca
    14521 ccgcagcagt gttgctattt cgagcaacgg cgggttaatg cgacaccagc cccgatcagc
    14581 cccccgacaa tgccccacat aatcatcagg aaggcacgac gtgggctatc gcgttttacc
    14641 ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac gcggatccag ggttggacca
    14701 acattcaggg tgtttaacat ggcccgattc tgatcatagt cgagatcaaa ggccggaccg
    14761 acggcctgta aattttccag tcgagcctgg agcattggac gcccaagcag gaacatttct
    14821 gaatcaggta attcctcggc aggcacatct gtcgcactgc gcgaaatatt atgctgctca
    14881 gcaattttca gcgcctgttc aatactgttc atccggcggt cgtagatggc tttcgccacc
    14941 tcttcctgac gcttcacctg tgctttcatc tggatggtac gcgccgccca tgcgcctttc
    15001 agctcatcat tcagatggct ggctgcacgc tggctggcaa aagcaacata ctgacgtaac
    15061 aggttattag cgtcaggcgc ggtttcagca ataagcttca cgctgtcatt gaccgcgcgg
    15121 gtaaagtctc cggggataaa ctggatgttg ttaatcattt catccagcaa cgccgcatcg
    15181 gctttgctgt tgcccaccat ccgctgtttg taatagtcgg tttgcagcca gaactctctg
    15241 cgggtatccc acgaggccag ttgcataaca aactctttat aggcttcgtc catgaccgat
    15301 ggttggtcgg cagaagccat gtttgaacgg acatccaggt tacgcaaaaa ttgctgctgc
    15361 gagtaatatc cccccagcat attcaccgtt ggacgatcgg taatcgccgt cgagctccac
    15421 tcctgacgag caaaaaaagt atacgccagc gcgattaacg caaatgccag ccccatgcca
    15481 ataatccata gcttcccagc ccacaaggta cgaaacaacc cacgaatatc cagttcattt
    15541 tcagcgtctt cggccggttt cccaggcatt ggttgtgtca tcacatcctc atttatttgg
    15601 ttaaattggg gctgccacca cgatttctac gcagtctgcg ttttacgcgc ttaataaagc
    15661 gagcaacttt ccaggcacgc ttaatgcaat atccatagag gaagaatgct agcaaaaaga
    15721 gcaccagcat gacccactcc gggacaaaat gagaatattc tgccagcacg ccaatggaag
    15781 cgagcagtgc cgcagcaagg gtaatcagca caaacgcctg acgggaagta aacccggcac
    15841 gcatgatcaa atggtgaata tgctgacggt caggagagaa tgggctcatg cctttacgca
    15901 ggcgacggta cataatcgcc accatatcca ttagcggaat ggcgattatc cacaaagcgg
    15961 taaccgggct gatgggatgg gttttgccct gggtcgtttc gagcaggatc cagataacgg
    16021 taaaaccaat cagcgtactg cccgcatcac ccataaagac tttgtagcgg cgacccagga
    16081 taccaaggtt aagcatgatg tatggcagga tggcggcgat catcgcaaag caccagattg
    16141 cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat tgctgcaaac gagacgcagg
    16201 acaacccgcc cagcaagcca tcaatgccat caaccatgtt gaacgcatta atggccgccc
    16261 agacggcaaa tagcgtcagg aagtaaccaa acggtccgag caccatctcc caggagccaa
    16321 agatataacc caggctactg agataaagtt tgccgaacac catcataaca atgccaacag
    16381 cggcctgtat ggtggcacgg atttttacgc tgatatcaaa acggtcatcc agcgcgccaa
    16441 tgaaaacaag cacaccggca caagcgagat agagagatgc atgcggaata tagtaatcga
    16501 caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat ccccccaacg agaggtatca
    16561 atccctggtg acgtttgcgg aagtttggtt tatccactaa accgactttt tttgccacct
    16621 tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat actgatgaga tcagtactca
    16681 ctgtcagtaa attcacaatg catgctctca gagaaaatta ttagcagaag tataaccacg
    16741 aagaccttta ttcagaaggg aaagtccgtt ccgaacccac cagtcctgtg caaaaattaa
    16801 ccatttgata tgcaaattgc ataacacgcc agcaaattcc aggaattttc ctaacatcgc
    16861 cagcgcggca ttaagattac agatataaaa gcaaaacgcc acgtaaacac gtggcgtttt
    16921 tggcataaga caaatttatg agcgtttcat catttcgaag aaatcgtcat tggtcttggt
    16981 catggccagt ttattaatga ggaattccat tgcatcgatt tcgcccatcg ggtgaatgat
    17041 tttgcgcagg atccacattt tctgcagttc ttcctgagta gtgagcagtt cttctttacg
    17101 ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt ttttcagcga tcttacgaga
    17161 gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg tagataactt cgtccatttt
    17221 agaaccggta tcgataagcg ccgtcgcgat aatagtcagg ctgccgccct cttccacgtt
    17281 acgcgccgca ccaaagaagc gtttcggacg atgcagggcg ttggcatcca caccaccggt
    17341 caacacttta cctgacgccg gaacaacggt gttgtaagcg cgcgccagac gagtgatgga
    17401 gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg cgtttggcct tctcgatcac
    17461 catttccgca acctgaacgt ggcgagatgc gggttcgtca aaggtagaag caacaacttc
    17521 accttttacc agacgctgca tctcggttac ttcttccgga cgttcgtcga tcagcagaac
    17581 catcagcaca caatccgggt ggttgtaagc aatgctctgg gcaatgttct gcagcagcat
    17641 ggttttaccg gctttcggcg gtgccacaat cagaccacgc tgaccacgac cgataggtga
    17701 tgccagatcc agtacgcgag cagttaaatc ttcagtagaa ccgttaccac gttccatacg
    17761 cagacgagaa tttgcgtgca gcggggttaa gttctcaaag aggattttgt tgcgggcgtt
    17821 ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc gcaaaatagc gttcaccttc
    17881 tttcggcggg cgaatcttac cagagatggt atcaccagtg cggaggttga aacggcggat
    17941 ttggctaggg gaaacgtaga tgtcatcagg accggcgagg taggagctgt ctgcggaacg
    18001 gaggaaacca aatccatcct gcaatatctc cagtacgcca tcaccaaaga tatcttcgcc
    18061 actctttgcg tgctgcttca ggatggcaaa aataatgtcc tgcttacgca tgcgagccag
    18121 gttttccagc cccatatttt cgccgagagt gatcagctca gaaaccggcg tattctttaa
    18181 ttcggtaaga ttcatagtgg tgtgagttct taaacttggg gtaaatctcg aacttaatgt
    18241 tgtgaatggt atggcaggat catccatgcc tgtttacggt catcgactca tgtcttttcg
    18301 ctgcctggtc acaggagagt acgcagaact gaaacgacaa gacggattga gtgacgagcc
    18361 agaaatctgt tcacttcgca tttaagataa aacgggaagc gttggaaatt acaagattca
    18421 aacttaataa ggtatgttta atacgaagtc aacactaagt tagcatgact cacgccgggc
    18481 gtccagtttt tagcgacggg gcacccgaac atgaaattcc cttacgccag gttagcgtcg
    18541 aggaactctt tcaactgacc tttagacagt gcgcccactt tggttgccgc cacttcaccg
    18601 tttttgaaca gcagcagagt cgggatacca cggatgccat atttcggcgc agtgccaggg
    18661 ttttgatcga tgttcagttt tgcaacggtc agtttgccct gatattcgtc agcgatttca
    18721 tccagaatcg gggcgatcat tttgcacgga ccgcaccact ctgcccagaa atcgacgagg
    18781 atcgccccgt ccgctttgag tacatccgtg tcaaaactgt cgtcagtcag gtgaataatt
    18841 ttatcgctca tatataactc cacaggaata agcctggcgt gttggtttcg ttgttggtgt
    18901 aacattaacc aactaaaggt tgactttatt tcaccggata cgctttcgta aagcaatagt
    18961 aagctgatat tctaccacac tatgagcaaa acacatttaa cagaacagaa gttttccgac
    19021 ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa aagggtttca taactgtacg
    19081 cccattcagg cactggccct tccgctgacg ctggcgggtc gtgacgtagc cgggcaggcg
    19141 caaaccggta ccgggaaaac gatggcgttt cttacgtcaa cgtttcatta tcttctctct
    19201 catcctgcga ttgccgatcg caaggtgaat caaccgcgtg ccttaattat ggcaccgacg
    19261 cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc tggcggaagc tactggcctg
    19321 aagctgggtc tggcttacgg tggtgatggc tacgacaaac agctgaaagt gctggaaagc
    19381 ggcgttgaca ttctgattgg caccacgggt cgtttaattg actacgccaa gcagaaccac
    19441 attaacctcg gtgccattca ggtagtggtt ctggatgaag ccgatcgcat gtacgatctg
    19501 ggctttatta aagatatccg ctggctgttc cgccgtatgc cgcctgcaaa ccagcgcctc
    19561 aacatgctgt tctccgccac gctttcgtac cgggtacgtg aactggcgtt cgagcagatg
    19621 aacaatgccg aatatattga agtggaaccg gaacagaaaa cgggccaccg tattaaagaa
    19681 gagcttttct acccttctaa cgaagaaaaa atgcgtttgc tgcaaacgct gatcgaagaa
    19741 gagtggccag acagagcgat tattttcgcc aataccaaac accgttgtga agagatctgg
    19801 ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga caggcgatgt cgcgcagaaa
    19861 aaacgtctgc gtattcttga tgaatttacc cgtggcgatc tggatattct ggttgccacc
    19921 gacgttgccg cgcgtggttt gcatattccg gcagtgacgc acgtctttaa ctacgattta
    19981 cccgatgact gtgaagatta cgttcaccgt attggtcgta caggtcgcgc aggcgcaagc
    20041 ggtcactcta tcagcctggc gtgtgaagag tatgcattga atttgcctgc tattgaaacc
    20101 tatattggtc actcaattcc ggtaagcaaa tacaatccgg acgcattgat gaccgatctg
    20161 ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg gtccgcgtcg tactggcgct
    20221 ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata ctcaaaatca ttcaaactgt
    20281 atcaagatgg caagagaatg aatcccgatg agcatactgt agtaagtgat tcaggtgaaa
    20341 gaatgcagcc aacacagaga cagattgaag gatgaagagt atgggttcca cctcgtcgct
    20401 gtatgcagcc attgatctcg gttcgaatag ttttcatatg ctggttgtgc gcgaggtggc
    20461 tggaagcatc cagacgctga cgcgaataaa acgcaaagtg cgtctggctg ctggcctgaa
    20521 tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt tggcaatgtc tgcgcctgtt
    20581 tgctgaacgt ctgcaagata tccctccctc gcaaattcgc gttgtcgcta cggcgacatt
    20641 acgtcttgcc gtcaatgcgg gtgattttat tgccaatgcg caggaaatcc tcggttgtcc
    20701 ggtacaggtg atcagcggtg aagaggaagc acgtctgatt tatcagggcg ttgctcacac
    20761 aactggcggt gccgatcagc gcctggtggt ggatataggc ggtgccagta ctgaactggt
    20821 aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg tcgatgggct gcgtcacctg
    20881 gctggaacgc tattttgccg atcgtaatct ggggcaggaa aattttgatg ctgcagaaaa
    20941 agcggcacgc gaagtgttac gtccggttgc cgatgaatta cggtatcacg gctggaaagt
    21001 gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa atcatgatgg cacaggggat
    21061 ggatgaacgc attaccctgg aaaagttgca gcaattgaaa cagcgagcca ttcattgcgg
    21121 tcggctggag gaactggaga ttgacgggct gacgctggaa cgtgcgttag tgttcccgag
    21181 tggtctggcg atcctgatcg ccatttttac cgaactgaat attcagtgta tgaccctggc
    21241 gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg catctggccg tcgagcagga
    21301 tattcgcagc cgtacgctgc gtaatattca gcgccgcttt atgatcgata ttgatcaggc
    21361 gcagcgcgta gccaaagttg cggctaactt cttcgatcag gtggaaaagg aatggcatct
    21421 tgaagcaata agccgcgatt tgctcatcag cgcctgtcag cttcatgaaa tcggcctgag
    21481 cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg gtgcgtaatc tggatcttcc
    21541 cggttttacc cccgcacaga aaaaattgct ggcgacgcta ctgctcaacc agactaatcc
    21601 ggtcgatctc tcatcgctgc atcagcaaaa tgccgtaccg ccgcgcgtcg cagaacaact
    21661 ctgccgttta ctccgcctgg ccatcatttt tgccagccgt cgccgtgacg atctcgtgcc
    21721 agagatgaca ttacaggcta accatgaact gttgaccttg acgcttccgc aaggttggct
    21781 aacccaacat ccgctgggta aagagattat tgctcaggaa agccagtggc agagctatgt
    21841 ccactggccg ctggaagtgc attaatgagt aagtgccgga tgcgatgctg acgcatcttt
    21901 tccggccttg attatttccc tcgttttgcc gccatcatcg ctttcagatt cgccagatgg
    21961 ctttgccctt tctgcatccg ttcttcggcg ctgaccactt tgcgctcctg ttcccaaatc
    22021 agatcatcct gcggcagctc cagcaaaaag cggctcggct ccgggcgcac cagctcgccg
    22081 tactgacggc gttctttaca cagcgtaaag gtcaattcct tctgggcgcg ggtaatgccg
    22141 acataggcca gccgccgctc ctcatcgata ttatcttcat cgatgctgct ctggtgcggc
    22201 aaaaaccctt cttccatacc gaccatgtag acataaggaa actccagccc ttttgacgcg
    22261 tgcagagtca tcagttgcac ctgatccagc tcttcttcac tctcaccacg ctccatcatg
    22321 tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca tcggctcatc cagttcgctg
    22381 ccttccagca tctccgtcat ccagctaaac agttggttga cgttcttcat gcgcatttcg
    22441 gcggctttcg ggctgggcga tgtttcgtac agccaggatt cataatccat gccgtggatc
    22501 agatcgcgca ccgcggcaat cggctcccgc tccgccagac gctggatttc tgccaaccag
    22561 tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa gcgtctgact caggcccata
    22621 tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg cccactcacc cagctttttc
    22681 agcgtcgccg ggccaatctc tctcttcggc gtgttaacga tacgcagaaa tgcgctgtca
    22741 tcgtccgggt tagtcagcac gcgcagataa gccagcaagt ccttgatttc aggacgagag
    22801 aaaaacgacg taccaccaga aattttgtac gggatgcggt tttgcatcag gaatttttca
    22861 aacactcgcg actgatggtt accgcgataa agaatagcgt aatctttgta ctgcgtttta
    22921 ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct cggcttcatg ttcttcgtta
    22981 ttcgcgctta aaactttaag ctctgtgccg taacccagtt cggagaacag acgcttctca
    23041 aagacgtgcg ggttattggc gatcaggatg ttcgccgctt tcaaaatacg tccggaagag
    23101 cggtagttct gctcaagctt aatcaccttc agcgccggaa aatcctgact cagcagcacc
    23161 aggttttgtg gacgtgcgcc gcgccaggag tagatcgact ggtcgtcatc gcccaccacg
    23221 gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt catactggct ggtgttggtg
    23281 tcctgatact catccaccag cagatagcga attttgttct gccagcgctc gcggacttct
    23341 tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat catcgaagtc gagaacattg
    23401 cacgctttca ggtgtgcatc atacagccca taacaatggg caaaaatacg gtcccgctcg
    23461 ccaatcgcac tggctgccgc ctgggacggt gttttgagat cgttcttcca gttagagatg
    23521 gtcgaaatca actgttgcag gagaactttg tcatcttcaa tcagcccttc ggtcaactct
    23581 ttgagcaacg ccagctgatc ggtatcgtca aacagcgaaa agttcgcctt catccccagc
    23641 gccgcatact cacgcttgat gatatccagc cccagcgtgt ggaaggttga gatcatcagc
    23701 ccacgcgcct ctttgcgacc cagcgtctgt ccgacacgct ctttcatctc gcgcgccgcc
    23761 ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat aaccgcaacc gcggatcaga
    23821 tgggcgattt tattggtgat cacacgagtt ttaccggaac ccgcgcccgc cagcaccagg
    23881 caggggccgg taacgaattc gacagcttgt tgttggccgg ggtttagacg cataggtgta
    23941 ttgctcaatc ttcgaacggg gggaggattg tagcagaatc gcatttgaat gtgaccgcgt
    24001 gtaagccgct aaccgattgg ctgcggatgt ttaaatcaga gagggaagac cgaaaccgga
    24061 gttcatcgtg aatccattag acttagaaaa tatcgggtcg taacgttgtg ttattacttt
    24121 ttcagagttt actaaggaga ggtgtactcg tggcgattaa tttcagtcct aaggttggtg
    24181 aaatactgga ttgcaacttt ggtaactatc ctgttagcca gaatggacag ttcagcacca
    24241 cgtattacga tggccgcatt cctcctgaaa tgataaaaaa caggttagt
//
LOCUS       DAZSBF010000044        24279 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZSBF010000044 DAZSBF010000000
VERSION     DAZSBF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.044"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(123..1004)
                     /gene="prmA"
                     /locus_tag="ACHXWD_001377"
     CDS             complement(123..1004)
                     /gene="prmA"
                     /locus_tag="ACHXWD_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR7620337.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1016..2467)
                     /gene="panF"
                     /locus_tag="ACHXWD_001378"
     CDS             complement(1016..2467)
                     /gene="panF"
                     /locus_tag="ACHXWD_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR7620338.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2457..2699)
                     /locus_tag="ACHXWD_001379"
     CDS             complement(2457..2699)
                     /locus_tag="ACHXWD_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR7620339.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2808..4157)
                     /gene="accC"
                     /locus_tag="ACHXWD_001380"
     CDS             complement(2808..4157)
                     /gene="accC"
                     /locus_tag="ACHXWD_001380"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR7620340.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4168..4638)
                     /gene="accB"
                     /locus_tag="ACHXWD_001381"
     CDS             complement(4168..4638)
                     /gene="accB"
                     /locus_tag="ACHXWD_001381"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR7620341.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5616..6590)
                     /locus_tag="ACHXWD_001382"
     CDS             complement(5616..6590)
                     /locus_tag="ACHXWD_001382"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR7620342.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6742..8682
                     /gene="csrD"
                     /locus_tag="ACHXWD_001383"
     CDS             6742..8682
                     /gene="csrD"
                     /locus_tag="ACHXWD_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR7620343.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAAQLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8987..10030
                     /gene="mreB"
                     /locus_tag="ACHXWD_001384"
     CDS             8987..10030
                     /gene="mreB"
                     /locus_tag="ACHXWD_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR7620344.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10096..11199
                     /gene="mreC"
                     /locus_tag="ACHXWD_001385"
     CDS             10096..11199
                     /gene="mreC"
                     /locus_tag="ACHXWD_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR7620345.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11199..11687
                     /gene="mreD"
                     /locus_tag="ACHXWD_001386"
     CDS             11199..11687
                     /gene="mreD"
                     /locus_tag="ACHXWD_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR7620346.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11696..12289
                     /gene="yhdE"
                     /locus_tag="ACHXWD_001387"
     CDS             11696..12289
                     /gene="yhdE"
                     /locus_tag="ACHXWD_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR7620347.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12279..13748
                     /gene="rng"
                     /locus_tag="ACHXWD_001388"
     CDS             12279..13748
                     /gene="rng"
                     /locus_tag="ACHXWD_001388"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR7620348.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13816..17616
                     /gene="yhdP"
                     /locus_tag="ACHXWD_001389"
     CDS             13816..17616
                     /gene="yhdP"
                     /locus_tag="ACHXWD_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR7620349.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17772..19217
                     /gene="tldD"
                     /locus_tag="ACHXWD_001390"
     CDS             17772..19217
                     /gene="tldD"
                     /locus_tag="ACHXWD_001390"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR7620350.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19345..20210)
                     /locus_tag="ACHXWD_001391"
                     /pseudo
     CDS             complement(19345..20210)
                     /locus_tag="ACHXWD_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            20393..20596
                     /gene="aaeX"
                     /locus_tag="ACHXWD_001392"
     CDS             20393..20596
                     /gene="aaeX"
                     /locus_tag="ACHXWD_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR7620351.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20604..21536
                     /gene="aaeA"
                     /locus_tag="ACHXWD_001393"
     CDS             20604..21536
                     /gene="aaeA"
                     /locus_tag="ACHXWD_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR7620352.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21542..23509
                     /gene="aaeB"
                     /locus_tag="ACHXWD_001394"
     CDS             21542..23509
                     /gene="aaeB"
                     /locus_tag="ACHXWD_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR7620353.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23601..23873
                     /locus_tag="ACHXWD_001395"
     CDS             23601..23873
                     /locus_tag="ACHXWD_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR7620354.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23929..24192)
                     /gene="yhcN"
                     /locus_tag="ACHXWD_001396"
     CDS             complement(23929..24192)
                     /gene="yhcN"
                     /locus_tag="ACHXWD_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR7620355.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5754 a         6134 c         6572 g         5819 t
ORIGIN      
        1 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata accggcaaat
       61 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
      121 ggttaattct tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt
      181 gcgaagctgt cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
      241 aggcccagca aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat
      301 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
      361 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctagcc
      421 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
      481 gcgatagcca gaatgccgga accacagcca aagtcgatga ctattttacc ggttaaatcg
      541 aggctgtcga gccactgcag gcacagagag gtagttggat gggtacccgt accaaacgcc
      601 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
      661 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
      721 cagtctttat cttccagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
      781 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
      841 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
      901 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttca
      961 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     1021 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     1081 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     1141 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     1201 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     1261 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     1321 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     1381 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     1441 tttgtagcaa ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     1501 tcccggcagc aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta
     1561 cggtgagatc ggggatcact gcacgaccta acgctccggc taggtgcata ccgaacatca
     1621 ggattgcgac tacaatcgta ccgatgatga tcccccggtg taccgctttg ctgtctttat
     1681 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     1741 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     1801 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     1861 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     1921 gcacgctggc gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca
     1981 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     2041 actgcacggt cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     2101 gaagacgact ctggtagcgg gcaaacagca tgtcgttcag cgtcactgca ttgtagcggc
     2161 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagact gcaggaagct
     2221 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     2281 taaacgaact ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca
     2341 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     2401 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
     2461 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
     2521 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
     2581 gtaaaaccgg gggcaacgcc aggtaagtaa gcggctacta accaaactgc cagatacaaa
     2641 agggtcagcc ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt
     2701 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
     2761 gtaataaaaa aggccggaaa atccggcctt ttggcgcttt tgcagtctta tttttcctga
     2821 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
     2881 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
     2941 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
     3001 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
     3061 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
     3121 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
     3181 tcttgcttga tcgacagcgg ttgaccggca gcgatacgga gctgttcttt gatcaggtca
     3241 acgccagtga tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
     3301 aaatagaact cgccgttttc gaacagaaac tcgaaagtac ctgcaccgcg atagccgata
     3361 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
     3421 ggtgctggcg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
     3481 gccagataga tagcgttgcc ccgaccgtca gccagtacct gaatctcgac gtggcgagga
     3541 ttttccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
     3601 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
     3661 ccaccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggca
     3721 cggtttttat ccatatcgtc gcccaacggg ccgtcagaac ccggtacgca agggacgccc
     3781 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
     3841 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
     3901 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
     3961 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
     4021 tctgccagta atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg
     4081 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
     4141 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
     4201 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
     4261 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
     4321 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
     4381 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
     4441 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
     4501 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
     4561 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
     4621 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
     4681 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
     4741 gcaggccggg acgccggtct attttgcccc agagtatcaa tgttagactt gacggacatt
     4801 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
     4861 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
     4921 ttcgtagcaa ttggcagcta aatactggtc tcatcaggga agataatcaa cagctaacat
     4981 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
     5041 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag
     5101 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
     5161 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
     5221 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc gactgacaat tctcagcatt
     5281 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
     5341 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
     5401 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
     5461 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
     5521 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
     5581 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
     5641 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
     5701 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
     5761 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
     5821 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
     5881 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
     5941 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
     6001 gggagaatac ggctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
     6061 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
     6121 ccactggcac ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc
     6181 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
     6241 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
     6301 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
     6361 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
     6421 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
     6481 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gactttcgtc cagagtctgt
     6541 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
     6601 catgtgcatg atggattgga aaataatttc tgaagattat actcgctaat ggaaaagcaa
     6661 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
     6721 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
     6781 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca
     6841 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
     6901 gtgtcgaatg acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat
     6961 atcgttcgtg tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt
     7021 agttatcgtc cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag
     7081 catccgggga tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct
     7141 ttaatgacta ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg
     7201 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
     7261 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
     7321 agaaccagca gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
     7381 agccgtcttg atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat
     7441 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
     7501 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgacacttgg
     7561 gggcacagcc aggttgaaga acagttcttc actctgacta atctgctgtc gacatttatg
     7621 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgcagtta
     7681 ccgcaccgga cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat
     7741 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc
     7801 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
     7861 gcgggattgc agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga
     7921 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
     7981 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
     8041 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
     8101 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
     8161 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt
     8221 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
     8281 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
     8341 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
     8401 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
     8461 agaaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
     8521 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
     8581 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
     8641 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
     8701 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
     8761 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
     8821 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
     8881 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
     8941 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
     9001 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
     9061 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
     9121 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
     9181 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
     9241 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
     9301 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
     9361 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
     9421 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
     9481 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
     9541 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
     9601 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
     9661 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
     9721 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
     9781 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
     9841 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
     9901 tttgttaatg gaagaaaccg gcattccagt agttgttgct gaagacccgc tgacctgtgt
     9961 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    10021 cgaagagtaa tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga
    10081 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    10141 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    10201 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    10261 cctcgtgaat tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt
    10321 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    10381 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    10441 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    10501 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    10561 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    10621 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    10681 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    10741 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    10801 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt
    10861 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    10921 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    10981 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    11041 acggggatcg ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct
    11101 gctgcgccca cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    11161 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    11221 gacgttgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    11281 cggataacct gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc
    11341 tggccttgcc tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    11401 tgatcagcgg ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    11461 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    11521 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    11581 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    11641 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    11701 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggtgt
    11761 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    11821 gcagtatgtt gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca
    11881 ggatctcccg gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa
    11941 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    12001 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    12061 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    12121 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    12181 gataaatggc agctatcacg ccgtagtcgg cttacctctg gttgaaacgt atgaattatt
    12241 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    12301 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    12361 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    12421 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    12481 ttcatgcatc cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat
    12541 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    12601 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    12661 gctatctggt ctttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg
    12721 aatcagaacg tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    12781 ttatcatccg taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    12841 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    12901 agctgtacgg cgaactggcg ctggctcagc gtgttctgcg tgatttcgcc gacgccgaac
    12961 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attacttgag ttcacctcgg
    13021 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    13081 atctctttga tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat
    13141 ccggtggtta tctcattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    13201 gagcgtttgt cggtcatcgc aatctggacg acaccatttt caataccaat attgaagcga
    13261 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    13321 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcactcgctg gagcaggcgt
    13381 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    13441 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    13501 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    13561 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    13621 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    13681 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    13741 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    13801 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    13861 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc
    13921 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    13981 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    14041 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    14101 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    14161 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    14221 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttc
    14281 ctgacgccat ccggtcagcg tgccgagctg gcgatcccac aactcacctg gctgaacgat
    14341 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    14401 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    14461 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    14521 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    14581 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    14641 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    14701 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    14761 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    14821 gaactccgga ttcgcgccag taatctggag ctggcaggcc tggagggcat acgcccgctg
    14881 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    14941 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    15001 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    15061 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    15121 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    15181 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    15241 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    15301 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggca ctatttcccg
    15361 gaaaacttga tgggtaaaga cctggttgat tacttaagtg gcgcgattca gggcggtgaa
    15421 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    15481 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    15541 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    15601 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    15661 gactactcga aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    15721 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    15781 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg
    15841 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    15901 agcaccctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    15961 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    16021 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    16081 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    16141 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    16201 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    16261 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    16321 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    16381 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat
    16441 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    16501 gtcggtggtg cagcgagttt cccacaatac ataacgttac gtacgcctat gttgtcactg
    16561 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    16621 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    16681 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    16741 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    16801 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    16861 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    16921 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    16981 ggaaaactgc gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    17041 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    17101 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    17161 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    17221 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    17281 cgcagcaccg cgtggattaa agacggggtt atgcacaccg acgacacgct ggtggatggc
    17341 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg
    17401 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    17461 aaccccattg ttggcgcggc agtttttgcc gctagtaaag tgctggggcc gctgtggagc
    17521 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    17581 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    17641 caatctcata tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggccgga
    17701 tagggcgttc atgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    17761 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacagcctga
    17821 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    17881 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    17941 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    18001 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    18061 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    18121 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    18181 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    18241 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    18301 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    18361 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    18421 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    18481 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    18541 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg
    18601 gcacttcagt atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    18661 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc
    18721 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    18781 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    18841 atctgcccat gccgcgtatg actaacacct atatgctgcc gggtaaatcg accccgcagg
    18901 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    18961 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    19021 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    19081 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    19141 aagggcaaag tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg
    19201 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    19261 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    19321 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    19381 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    19441 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    19501 cggcagcagg atctccagct caccgcgatt gatctcgttg atcacccaca tcagcggcac
    19561 gtaggcgatc ccggcacccg ccgtcagcca gcgcaccagc gtcatcggat cattagtcac
    19621 aaatcttcct tgtgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc
    19681 attgtcgggc cgcacgctgt attcaagcca tgaatgacta ctcaaatcgg cgggtttttc
    19741 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccatcg gcatcgcgcc
    19801 cagacggcgg gaaaacaggc tggaattccg gtaaccagat tgacgctcaa acctgggtat
    19861 tctttcagca tttttgctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag
    19921 ccaatgcgca gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc
    19981 tgcacttcat gaagcatccg acggcagccc tggtagtaaa ttctgccggc ttcggtcagg
    20041 ccaatgctgc gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac
    20101 accgtctgac tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag
    20161 ccaaattcaa ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct
    20221 gacttaaaag tgatttagat cacataatat agataacagc ataacagtta cgctaatata
    20281 ttaaatatca atctacagca atgttgctct cgcccggctt tccatgccct tctctgcggc
    20341 gacagatgct gaaaataata acgcctgctc tctctttaca accaaggtca acatgagtct
    20401 gtttcccgtt atcgtggtgt ttggactgtc cttcccaccg atattttttg aattgctttt
    20461 atcactggcg attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt
    20521 tgtctggcat ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc
    20581 gcgactgttc gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca
    20641 tcacggtcgt attagtcatt ctagccttca tcgcaatttt taatgcctgg gtctattaca
    20701 ccgaatcccc ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg
    20761 acgtttctgg actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac
    20821 agatactgtt caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg
    20881 atgttgctta ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc
    20941 gtctcggtgt gcaggcgatg tctcgcgaag agatcgacca ggccaacaac gtactacaaa
    21001 cggttctgca tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg
    21061 aacgcacggt gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg
    21121 gtgagtttat tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg
    21181 tactggccta tatggaagaa actaagctgg aaggggggcg tccggggtat cgtgcagaga
    21241 tcacgccgct tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgcagggg
    21301 tcaccaacgc cagcagcacg cgtgacgaca aagggatggc gactatagac tctaatcttg
    21361 aatgggtgcg tcttgcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga
    21421 acatctggcc tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg
    21481 aaagccagga ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac
    21541 gatgggtatt ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc
    21601 cattgtactg gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt
    21661 actgacagcg gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc
    21721 tggcgctatt cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc
    21781 cggactggtg atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg
    21841 tatctgggcc ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc
    21901 gtgggggctg gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt
    21961 gcttacgccg cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc
    22021 gattatggcg gatttgctct tttctccgcg atcgatcaaa caagaagtag atcgagagct
    22081 ggaaagtttg ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg
    22141 tgaagttgtc gataaagcct ggggcgacct ggtgcgacgc accacggcac tacaaggcat
    22201 gcgcagcaac ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc
    22261 gatcaatacg ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa
    22321 tacgcgcccg gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac
    22381 cgcgcaggac gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga
    22441 acgggaaacg cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct
    22501 caagcgcggc gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg
    22561 cgaaccggaa gtaaaagtag agtcagccga acgtcatcat gcaatggtta acttctggcg
    22621 aaccacactt tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg
    22681 cagtggtgca atggtgatga ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc
    22741 acgcatggtg gcgatcgact ttatctatgg gacgctggcc gcgctgccgt tagggctgct
    22801 ctactttttg gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct
    22861 ggcagtgctg ggattcttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg
    22921 ggcactggcc agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag
    22981 tcagtttctc gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat
    23041 tttgctggtg cgggataaat cgcgcgacag gactggacgt gtactgctta atcagtttgt
    23101 ttttgccgct gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc
    23161 ggcactttat cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt
    23221 tcgcctggcg ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt
    23281 taacgaggat ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc
    23341 tgcccgtagc gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat
    23401 ctaccaggaa aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg
    23461 gctggcgggg atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga
    23521 cgccaaaagc gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg
    23581 aaacgtgacg gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc
    23641 aggaggattt ttatcgtgac tttagccaaa cctttggtct ggctaaagat aaggtacgcg
    23701 atctcgactc actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg
    23761 aatttgttca tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg
    23821 atgaggcaga ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa
    23881 aaaagccccc gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag
    23941 ttcagccgta gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc
    24001 gcctttctct tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac
    24061 accacttacg gatacggtcc cgattgcttc acgattttgt gcttgtgcag tatcaatgga
    24121 gtcggcagcg aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt
    24181 tttgattttc atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgatc
    24241 catatcacaa aatcaagcat acactaatta ttgaactaa
//
LOCUS       DAZSBF010000045        24195 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZSBF010000045 DAZSBF010000000
VERSION     DAZSBF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24195
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.046"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            520..759
                     /locus_tag="ACHXWD_001397"
     CDS             520..759
                     /locus_tag="ACHXWD_001397"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR7620356.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHXWD_001398"
                     /pseudo
     CDS             complement(947..1924)
                     /gene="intI2"
                     /locus_tag="ACHXWD_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHXWD_001399"
     CDS             2256..2729
                     /gene="dfrA1"
                     /locus_tag="ACHXWD_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR7620357.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2824..3348
                     /gene="sat2"
                     /locus_tag="ACHXWD_001400"
     CDS             2824..3348
                     /gene="sat2"
                     /locus_tag="ACHXWD_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR7620358.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3417..3914
                     /locus_tag="ACHXWD_001401"
     CDS             3417..3914
                     /locus_tag="ACHXWD_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620359.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            3975..4346
                     /locus_tag="ACHXWD_001402"
     CDS             3975..4346
                     /locus_tag="ACHXWD_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620360.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4756..5133
                     /locus_tag="ACHXWD_001403"
     CDS             4756..5133
                     /locus_tag="ACHXWD_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620361.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5156..5308
                     /locus_tag="ACHXWD_001404"
                     /pseudo
     CDS             5156..5308
                     /locus_tag="ACHXWD_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5364..6980)
                     /locus_tag="ACHXWD_001405"
     CDS             complement(5364..6980)
                     /locus_tag="ACHXWD_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR7620362.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(6981..8507)
                     /locus_tag="ACHXWD_001406"
     CDS             complement(6981..8507)
                     /locus_tag="ACHXWD_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR7620363.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8510..10177)
                     /locus_tag="ACHXWD_001407"
     CDS             complement(8510..10177)
                     /locus_tag="ACHXWD_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7620364.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10174..12282)
                     /locus_tag="ACHXWD_001408"
     CDS             complement(10174..12282)
                     /locus_tag="ACHXWD_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR7620365.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12269..13090)
                     /locus_tag="ACHXWD_001409"
     CDS             complement(12269..13090)
                     /locus_tag="ACHXWD_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR7620366.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="ACHXWD_001410"
     CDS             complement(13250..15079)
                     /gene="glmS"
                     /locus_tag="ACHXWD_001410"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR7620367.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="ACHXWD_001411"
     CDS             complement(15241..16611)
                     /gene="glmU"
                     /locus_tag="ACHXWD_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR7620368.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(16962..17381)
                     /locus_tag="ACHXWD_001412"
     CDS             complement(16962..17381)
                     /locus_tag="ACHXWD_001412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR7620369.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="ACHXWD_001413"
     CDS             complement(17402..18784)
                     /gene="atpD"
                     /locus_tag="ACHXWD_001413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR7620370.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="ACHXWD_001414"
     CDS             complement(18811..19674)
                     /gene="atpG"
                     /locus_tag="ACHXWD_001414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR7620371.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="ACHXWD_001415"
     CDS             complement(19725..21266)
                     /gene="atpA"
                     /locus_tag="ACHXWD_001415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR7620372.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="ACHXWD_001416"
     CDS             complement(21279..21812)
                     /gene="atpH"
                     /locus_tag="ACHXWD_001416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR7620373.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="ACHXWD_001417"
     CDS             complement(21827..22297)
                     /gene="atpF"
                     /locus_tag="ACHXWD_001417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR7620374.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="ACHXWD_001418"
     CDS             complement(22359..22598)
                     /gene="atpE"
                     /locus_tag="ACHXWD_001418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR7620375.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="ACHXWD_001419"
     CDS             complement(22645..23460)
                     /gene="atpB"
                     /locus_tag="ACHXWD_001419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR7620376.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="ACHXWD_001420"
     CDS             complement(23469..23849)
                     /gene="atpI"
                     /locus_tag="ACHXWD_001420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR7620377.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         6295 a         6111 c         5436 g         6353 t
ORIGIN      
        1 tcggcagccc tgtgggcgga caaaatagtt gggaactggg aggggtggaa atggagtttt
       61 taaggattat ttagggaaga gtgacaaaat agatgggaac tgggtgtagc gtcgtaagct
      121 aatacgaaaa ttaaaaatga caaaatagtt tggaactaga tttcacttat ctggttaact
      181 aatctagcta acctgccgct cagcttagta cgatcctata tccaactgat cataatcctt
      241 tcatcagtat cccaaattaa atccccctcc tagactgcct caccaacact ttacagtgat
      301 cccgtatttc cggacaggtg attgattttt ttcgcttgta caggtgttaa gccacagtta
      361 tcttgatgtg gacattatgc attgtaatag cccattaaca tgcgctaaga gcataaatta
      421 atatacagta acaagcatta cgaggtgcta atgtttggaa agcagcctct tccaactaca
      481 ataataagtt aactatcctc cacccacaaa taattacaaa tgaccccttt ccgctataac
      541 tccgatctca ccagcggctc gctacaaacc cgagagtgtc gcattattac tggcttgtta
      601 ctccaagagc ttgatgaagc tgcctgggat aaagccatgt ataaggaaaa tgtgcttcag
      661 aaacgcacgc aatctacggt aagacgtatt tcttcagcgc ttagaaaacg cctagaacac
      721 ctaagctctg acttttgggc ttttgcgttt ttatgttagg cattgtctgg caactggagg
      781 aagtgtaatg accatgaatg gtcactgtga ttgacagtat cttgcttaaa cttatctgaa
      841 caatggtaga actggctcat atttatcccc tctatgattg tttatctttt tcaccataaa
      901 aagtatcagg ggctactcag ggctacataa agatatttat ctacttttac tgattgataa
      961 gtagcatcag tccatccgca ggactggtgg tgccggcaaa atgctgaccc aacacatgcg
     1021 tatagatttg cgtggtctta acatcgttat gccctaagag ttcttgcaca gtgcgaatat
     1081 cacgccccgc ttgtaataga tgcgtagcaa acgagtgacg aaatgtatga caagtgacac
     1141 gcttgctaac gatgcctgct ttttgtacgg ctgccttcaa tgcctttcgc gcaacggagt
     1201 catgcagatg atggcggcat aatttgccgt tatacgggtg gttgcagagc gtgctggagg
     1261 gaaagacaaa catccacgcc gcttgtcgat aagcagaagg gtatttgtga tctaaagcaa
     1321 aaggcagcga tggccctacg ccttgtaagt tgtcgtcttg ctgaataagc cgcgcttgct
     1381 caatgagtta ttttattgct gggattaggc gcgtgggcag taggctgttt ctgcttttcc
     1441 cacccttacc gtcatgcaca gtgatgcagc cattatcaaa atcaaaatct ttaacccgca
     1501 aacgcaagca ttcattaatg cgcaaacctg caccatacag cagcgtaaaa ataacttggt
     1561 tgcgagtatc cataacctgc aaaatgcgtt gcacttcatt tgcagagata acagagggta
     1621 gccgtctagg cttgcttgca gggatataat caatatcgcc caacggctgt tgtaaaaacc
     1681 tgttgtacaa aaaagctagg gcatttaaag cgattttctg cgtgtttatg gctacatgtc
     1741 tgctgtttgc taagctggat aaaaacagcc tgacctcttc actgcccatg gtctgaggat
     1801 gacgtttttt gtgaaacaga ataaaacgct taatccagtg caggtaagtt ttttcagttt
     1861 tcagcgcata acctttttgt cgcatatccg tgcgtataga atttaaaaat ggactgttag
     1921 acataaaacg ctccttgtct tgcaactgtc tgcctataca gcctattcta gctgggattt
     1981 aaaaaagtgc ctgtttttta cgcctagaga tgcttgttta ccggtagagt tttaatttaa
     2041 tgctaaataa attaaaatgt tatgagttct ttgggtgaga taatgtgcat cgtgcaagca
     2101 ggatagacgg catgcacgat ttgtaataac agagtgtctt gtatttttaa agaaagtcta
     2161 tttaatacaa gtgattatat taattaacgg taagcatcag cgggtgacaa aacgagcatg
     2221 cttactaata aaatgttaac ctctgaggaa gaattgtgaa actatcacta atggtagcta
     2281 tatcgaagaa tggagttatc gggaatggcc ctgatattcc atggagtgcc aaaggtgaac
     2341 agctcctgtt taaagctatt acctataacc aatggctgtt ggttggacgc aagacttttg
     2401 aatcaatggg agcattaccc aaccgaaagt atgcggtcgt aacacgttca agttttacat
     2461 ctgacaatga gaacgtattg atctttccat caattaaaga tgctttaacc aacctaaaga
     2521 aaataacgga tcatgtcatt gtttcaggtg gtggggagat atacaaaagc ctgatcgatc
     2581 aagtagatac actacatata tctagaatag acatcgagcc ggaaggtgat gtttactttc
     2641 ctgaaatccc cagcaatttt aggccagttt ttacccaaga cttcgcctct aacataaatt
     2701 atagttacca aatctggcaa aagggttaac aagtggcagc aacggattcg caaacctgtc
     2761 acgccttttg taccaaaagc cgcgccaggt ttgcgatccg ctgtgccagg cgttaggcgt
     2821 catatgaaga tttcggtgat ccctgagcag gtggcggaaa cattggatgc tgagaaccat
     2881 ttcattgttc gtgaagtgtt cgatgtgcac ctatccgacc aaggctttga actatctacc
     2941 agaagtgtga gcccctaccg gaaggattac atctcggatg atgactctga tgaagactct
     3001 gcttgctatg gcgcattcat cgaccaagag cttgtcggga agattgaact caactcaaca
     3061 tggaacgatc tagcctctat cgaacacatt gttgtgtcgc acacgcaccg aggcaaagga
     3121 gtcgcgcaca gtctcatcga atttgcgaaa aagtgggcac taagcagaca gctccttggc
     3181 atacgattag agacacaaac gaacaatgta cctgcctgca atttgtacgc aaaatgtggc
     3241 tttactctcg gcggcattga cctgttcacg tataaaacta gacctcaagt ctcgaacgaa
     3301 acagcgatgt actggtactg gttctcggga gcacaggatg acgcctaaca attcattcaa
     3361 gccgacaccg cttcgcggcg cggcttaatt caggagttag agagctgggg aagactatgc
     3421 gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat ggcatcgggg caggcacttg
     3481 ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc ctatgactac tccccatcca
     3541 actacgacat ttctccaagc aactacgaca actccataag caattacgac aatagtccat
     3601 caaattacga caactctgag agcaactacg ataatagttc atccaattac gacaatagtc
     3661 gcaacggaaa tcgtaggctt atatatagcg caaatgggtc tcgcactttc gccggctact
     3721 acgtcattgc caacaatggg acaacgaact tcttttccac atctggcaaa aggatgttct
     3781 acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga tgggagcttc tgcggggcat
     3841 tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga taacggcctg aagatcatgt
     3901 atctaagcaa ctagcctgct ctctaataaa atgttaggcc tcaacatcta gtcgcaagct
     3961 gaggggaacc actagtgtca tacgaacctc caagagacgg ttacacaaac gggtacattg
     4021 ttgatgtcat gtatgacaat cgcccaagta agtatccagc tgtgttcaga acgtacgtcc
     4081 gaattccaga ctatgtcgaa ctagataacg cggaggagct ggccgacgca atcattcgag
     4141 aggttgagtt aaaggaggca tggctcacaa gggtcttggg gaactcagcc gatcctgacg
     4201 cttctgtggg caagtgggac cggcgcctag cgcagtgcgg tggtattgat gagatgtcaa
     4261 gtggggtgca ggcaacatac ttcagtccaa acggtcagct ggtcggctta gtggcataca
     4321 ttgatgccga atggatggat gaatagctct gacgttacga ttcaggtgag acaaccagat
     4381 gggcattgcg tacaagctca gttgccaccg atgtggctac gagtctgatc ttctttacct
     4441 aggccaaggt atggcgatgc ttcctgagca aatagtcgga acgtgcacat gctgcgacaa
     4501 tctaacgacg atttctataa atgaaagcta cggagtatgc tcactttgtg gggctaagga
     4561 tcgtgtgata tttacaagct atcagcatga ggcaaggcca agaaatccct gctatctaga
     4621 gagaagtttc aaatatcaat gcccaagatg ccatgccttc agtatggagc ctccggatct
     4681 accggagatg ctttgggact aatgctccgc tttgcctcgg gtatccgttc gacctatgtt
     4741 taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct atgcactttg tgacaagttg
     4801 gaaagcaaaa aagttgatga agtcgtacga aatcttgttt ctagcggctg gaatattagg
     4861 gaagtggcct gggaagcttc gtcctttgat catgaaatgc cctactttga cacggggcgg
     4921 gacttcgata ttggatttcc tgacacgctg tcatccaaga ttgagttttt gaccgcgaat
     4981 ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc aggctctaat ttttgccaag
     5041 gcgtccaaac atgtcgccgc gctcaagaac tcattggatt actactatgg atcgtctgta
     5101 atcccccctc aaaaccacgg aggctatttg tagaaaattc cattaatatg aactcaaggt
     5161 gttccctacg gacgaatcag cgatgaaggt ggtgtacctg gctatccagg cggcgaccaa
     5221 gaaatggacg atgccgatcc gcaactggaa gccggccatg aaccgtttta tgatcgaatt
     5281 tggtgaccgt ttaaatggcc acctttaact gaccggcaaa agcacttaca caaaagggtt
     5341 tacaggctct ttcagtcatt cctttaatgc gtaaattgct ctctaaccac tctctcagcc
     5401 cacccttcca tatcctctct cgatacttgc agctccccca acccctttgg atgattcacc
     5461 cctctatgcc cgtcttgtcc atataattga tcaaacaaac tatttggcca attcaatgag
     5521 ctcttcacca ctccacgccg tatcttttca aaatcattcc gccatgtttc gctatcaacg
     5581 ccactcaaca ctttggtaga aagcattttc accccatccg atgcatccac ctctagcaat
     5641 acaaattcac tgccattacg ttgcacccca acggctttaa tcactcttgg tgagccatct
     5701 ttacacagat gtaatcggct acgcccaact tttggcaaaa ccaaggtttc ttcaaaaagc
     5761 acccgacatg caaatttagt ttttagaatt gaaagtagct catcaaaagc agcaaatcga
     5821 ttagcaaaaa tagagttgta attggttgca tcctgcttcc cacccacatc tgccgctgct
     5881 aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat tactattttc acttgttgtt
     5941 gcttcttctt tttctaaaat agtttggcta ctttttcttg ccctgcgaga tttttcaatt
     6001 cgactaggtc tactaaaact tatccatgtc ggctcagcct ctatgactcg tgtgtctgtg
     6061 tctatattgg ctgtttcctc atcgtccaac tgtagatgct catcatcaac aactgactct
     6121 gttgaaaccg ctatgtgctg cgtactacca tcacctgcct ccttttcctg aaaagaggca
     6181 tggctaatag ctgttgtgct aggaagcata gcgttgattt ctaaacctac tatttcctca
     6241 actaaataat ccttatcctc gttagaagaa cgtcctttta catgtaattt ccaaccctgc
     6301 atgggcggag ggtcaaagct aaagcaccag ctttcaacgc cgttcttaat ttctctattt
     6361 tgttgatagt gcctaaaaat actttcatac gaatccataa catcttgatc cgaaaacaac
     6421 caaaccaaaa gctgcactac ggccgactgc tctaacgccc ctttaggaaa cgatgaactg
     6481 ggtaagatcc ttatctctaa atgatctcgc tcaacctcat actgaacgtc gaactcttgc
     6541 tgtaaagcgg tactgctcaa acagcttcga cagaaatagg agttaattaa aaataatgac
     6601 cgagctagct ctaactgtgg gatgtgataa cgagtcttgc tatatggtgt ttcagcttcg
     6661 taaataaatg aactctgcgc tccatccttg ttgcgtacgg aagcaaagga cgagagatta
     6721 ggaaaatcga ttattttggc tcgttgaaga tccgatgctt caaactcaat cacacgatcc
     6781 gccttattta tttgctttgt tgtactgtta accaccttag ctcgacttaa taaaggcaaa
     6841 tgagtaaatc gagtccattt ccgttcttgc attggattaa accaaacaaa aatacgccac
     6901 tccttatgac ccgagttacg gaataaatga ccaatatgta caacctgcac attgtcatta
     6961 aatgtagcta gcctaaccac tcactcttcc ccataaacca tttccaagaa tctttgtgct
     7021 tcctcagtta tccgctcttt agataacgtt gcacttctta ataatcgcca gcgcctaagc
     7081 tcaacatctt cctgtttaag cttaataaaa gcttggctaa tccgtctaat ttgataatct
     7141 tccacactct ctgagtaacg ctttaagcaa agcgctacca aaggcagttt ctgtagattt
     7201 tttgcaagag aggttccgtt aggagtttgc tttaacagcc agctcgatgt cgctcttggg
     7261 tgatcaaggc tactatctag acgctttatg attcttaata gttgtcgtac agcaattcga
     7321 tctctttggt tccaatcaac tctaggggcg ggtgccagac gctcttgttg atgctgttga
     7381 ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc aagcgtatag caccccaccc
     7441 tctaaagact gtcttgccgc cttaattcct tggtatttat gcactagttg ctgccagtct
     7501 ttatgcttaa cagataaatc ttcagagtta ggttgcacag actggctaac aggtcttgtc
     7561 gttatagagt gctcagtaag agcacttgcc tgctgtagcg cttctataac cgttagtttt
     7621 ggcaataagg cttgccacac aatactatgc tgtaaataac taaaggcttt tctatgctta
     7681 cggaatatac ttttcagcca acacgtgtcc ttgttctctg ccaactttaa atccagtttc
     7741 tctagtgcct catcactaaa agtctgcctc actctctccg ccaccaagtc atgacgaatg
     7801 tgcttgcttt tggttagccc tagatcctgc gctaagcgct gataaaacag cgtccactgc
     7861 tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta acagaggggc tatataagca
     7921 gctagtgctg ttaattgaga tagggagtct ttggggtagt ctgaaagcag ctcagtatga
     7981 cccaaagccc aaaattgatg tcggtgatca tctacagctc tatcaaagaa gactaaagca
     8041 ccgtgttttg gacaatatgg caaagcgggc aaataccaat ctcgttgcca aaaggcttcc
     8101 ccatacctat ttagctgaag agcaacgcaa tcagggcagt atctaaagcg gttatcgctc
     8161 ttaactctag aagcagcgac tcctagcatt aaatgcaccg caccttgcgc ttggtactcc
     8221 attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa acggagcata taaagggaat
     8281 aaggtatgct cataaataag ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt
     8341 gctatcacac ctaaatgcga gggcagacct aaggtagcga ccaccttgcg gttgccatac
     8401 acctcatcca acagctgctt aggactaaca atcccttgat aaacgcctgc ccgtgcaata
     8461 gtgctataaa tcagctcatt cgagtacgga acaggaaagt ttctcatggc taacctgctt
     8521 gtttaaataa gctcgccata tccactatta cccctttccc ttttaaccgt tcatgcatgg
     8581 ttttttcagg ttggcgttgt gaatagatat aacgtaaatc cgtatcaggc aagctatccc
     8641 agtcgctggg cttgactacc tttatagccg aaaccttttt actcttggag ggcttttcta
     8701 gttctgacac taccgtttcg ccttccatca accactgcaa aacaagaggc agtaactttt
     8761 gtcttgtcat cgttggattc tggctaaacg cttttttaat agtgggaatt aacaggcttg
     8821 aatcgtaatc ctctttcagc atcagatata aatgacgctg atcttcggta tctaactctt
     8881 gaagggcttt ttcttctggt gtttgttctt gtatcgctgc gatatctagc tgaagttgga
     8941 ttaaccgttt atcaatctcg ggaacgacta gatcagaata acgagcaatg cgttctggga
     9001 tacccgagcg taatgcctct agcatggggt gcacaggctt taactcatct tgatacactt
     9061 gccgcaataa accagcggta atacgctcat tgcctaaagc tagcgcacgg agctgagcga
     9121 gtacaaaaag ttttactaca atgtccatca ctccttggct tagctcatac cacacatcac
     9181 ggacctcatc cgataacagc gcatctttgc gttgtaaaag ctgtaattgc caaagattat
     9241 ccgtaaaagc gatccactct tgattgggct ttccacgttg cgtttgttgt ataggatccc
     9301 agaatatagc tccaaaccct gcccctctac gtgcagaccg caaatcagcc tcaaaaatct
     9361 ctcgtgcttt aggggtacca atcaacatca ctggtacgcc aataatattc accatcgtca
     9421 caaaaaagtt cagcatctct tgagatccac ccgaacgaga gcggcttaaa tgctgaattt
     9481 catcaataac caacaaccct aaagcatgtg cattggctat ttgcgacatc aaagccaaca
     9541 tggtttctat accatgacgt tttaagccat aacgacgctc atagttcgag cccaaggctc
     9601 gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag cgaaccatta tgcgagcagt
     9661 ctattttcaa atacaccacc tgctctacat tgagttcacg atggtaaatc acctgaggat
     9721 acgtggctag aatacgatga agagaggtcg tcttcccact accagaacaa ccaattaaca
     9781 ataagctttg tgccgtagat cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt
     9841 gaacacgctc ataaccattt tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc
     9901 ctacgtagcc acctcgaatc atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta
     9961 atggctgaaa atagtcatct ggaatacgac aaatggtatg agctctgata acacgggact
    10021 tttgcaagtc agaggaagta agctgtaaag aggatttcag tgatgcagca ctattcactg
    10081 actcttgtaa tggtggtaag gcctcgataa aaggattatc acgataagcc tctacccccg
    10141 tatcacgata aactgcttga atccgggtag cactcatgac tcatcctttt ctggtggatc
    10201 ctgaaataat tcaggcacgt atgtgggtag gctgtaatct tcttgatcat ccgcttccac
    10261 tgcgttgaaa ggaataactt tagcctcatc acctgaagag cttggcttca aatgctccgc
    10321 acgtttttta cgctccgagg tcacggcttc ttttttatta gttttaatct gcttaatgcg
    10381 tgttgatttg ggctcagtag tactgggcgt taacttattc gctttctgaa tggtttgctg
    10441 aataaacgcc tcaagctccc tgcgtttagt taactcatcc tgcttcgcat tggctttatt
    10501 gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat gagagacctt taaactgccg
    10561 actacgttcc gtcagattac agcgccaaaa tacacggctg ccaacttgcg gaaacaaata
    10621 aatcgtatca accagcactg ggtcataagc cgcttctaaa tgttgaggtc tagctatatc
    10681 agtgctccgc tgcaaccaac cctcacgcag aatctctgac cccgagtaat acaaacccca
    10741 caaattaacg ccaaatgaag aaatagagac ctttcggcga ggcagtaacg ctactcgcaa
    10801 ctgctcttgc tccacagccc ttaaactacc tgtacgatgc tgcatacccc attgccatag
    10861 ctggacagga atagacggta aatctgtagg aaaatcagca tctcgatcgt atttatccat
    10921 caccagatgg ttatttctga ataagatcgt acgcaaaata atttgtgtga actcaaatac
    10981 cgacagagat gcatctaacc tatagtctgt ttcaccatgg cttttgatcc gactgccctc
    11041 tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt gttctaaaag tgctttccac
    11101 tatgccttta gcatcgccac gtctaggtgg agcactttcc actcgcacat taaaactaga
    11161 aactaaggct tcgacctgat gactcattaa ttcgccacgg tccgctagca acacatctgg
    11221 caaacctaca cacggccagt ctgagctact aatctcaata tcatgctggg cacaaatggc
    11281 cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc accacataag acggattttc
    11341 aaagccgata taaaagcccg tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt
    11401 tggtcttcct atgatttttt ggcgatcatg atgatccact aaataaatat cagcaatcgt
    11461 ggcatcaatc tcataacgac tcccagggcc taacgcctga gaagtggctg tactacttaa
    11521 gggtcgtacg tcttttttat atacccctgc tttaactcta gactttaagc gctgagcttt
    11581 agggtattct cgatcataaa aataacgaaa ctgacgtagt gttgggtaat cctcttgggg
    11641 aatgcgagga aaatactgag caaacaagtc cacaaatcgt ctataggcaa cggtggtctt
    11701 tgtacctttt tgatttaaca ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc
    11761 gggcgttacc ttggttcctt cacccttacc atattctctg gctcggccaa tctttgctgt
    11821 tcctgtcgct gaacgtcttt cccctggtgc accgctgttt ttgtagtcag gaattaatgc
    11881 attaggcgtt tgaccacgct gccagtaacg gcgtaacaac ttataaactg tagccttagt
    11941 aaccttatgt tcttggacca catgctcaac gagttcgctt ctgactttag ggtcgaaacg
    12001 atccttacta ttaataatag gaagaatttt tcgataatcc tcctcgcgct tctggaagct
    12061 gacagaatct acagacggct cttctaaatc aagatgtaca taagggtcat cagttctaac
    12121 taagcggcct tcatctaaat actgcattag caacaactca gccctagctt gtggaactcc
    12181 tttatcagcg cttatttgca tccagacaac ttggccatcc tctatagcca aaatgcgata
    12241 cggatcatta tcaaatagca caacctcatt aatttgccac atagcgcaac tcctccatat
    12301 tcactacttg gctaatacag agatctgcac acttatttgc cctgaaagac ttataaatat
    12361 tgaacttaat aaaaccattt gcggttaagg ctcgtatctc actcaatgtt ttgcctaact
    12421 ccaaatcata agcaatatca acctgcttac agacattgat aatgttttca tctccttttt
    12481 cttgcaggat atgggccaat ggggatagtt gtgctaaaag ctccgcagaa acttcttctg
    12541 ttttcactga ataaagccat tcaatatttt cttttactac gggatttatt tctttatcag
    12601 taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg acgctctagt tctagttttt
    12661 ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac ttgaatagca aactgctcaa
    12721 aaggaccatc tttgcagtcc actaaaaaat cagtagacat aacctgatct acaccacgaa
    12781 taacaggatg cttaatacca ctatctattg caatctgcct ggtatcacta ggtaataagg
    12841 ggaactgctc gcgtatatct agcacgctgc tctcccactc aagactgaga aaaacagcaa
    12901 gctctaagtc agatagcaaa tgatggactc gtcccgtctt atgagaataa atacggtggg
    12961 aacgacctga agaaggaact tcttgtactg ttagccatgg aatatagtct ttaccatgcc
    13021 cttggccacg cccctcttta atacgacggg caatttgcac ttcagaaaat gaagagtttg
    13081 ctttagccat aacaaaagtc cagtatgctt tttcacagca taactggact gatttcagtt
    13141 tacaactatt ctgtctagtt taagacttta ttgtcatagt ttagatctat tttgttcagt
    13201 ttaagacttt attgtccgcc cacaagccct tttttatcta tgatagtgat tactcaaccg
    13261 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
    13321 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
    13381 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
    13441 agacatacaa ttggccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
    13501 gcaattcgtt gttcggtgca acgacgataa ccggcatatc ggcatcaata agcgccagcg
    13561 gaccgtgttt cagttcacca gcagcgtagg cttcagcgtg aatgtaggag atctctttca
    13621 gcttcaatgc gccttccagt gcgattgggt actgatcgcc acggcccagg aacagtgcgt
    13681 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gatagcatct
    13741 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
    13801 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
    13861 tgaatgcttt agtggacgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
    13921 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
    13981 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
    14041 aggtgatcat caggctgtta cgacgcacgg cagatttacg atagcggaat tcagaggcga
    14101 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtagcgggaa accataccgg
    14161 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
    14221 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
    14281 tgttttttat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
    14341 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
    14401 cagttttatc gaagatgttt accgaacggc gagtgatttc cgcaatatcg ccctcttcaa
    14461 ggaagataaa gcgacgggtc accggcaaca gcgccagctg gtcagaagcg ataaagtttt
    14521 cgcccatacc caggccaatc accagcggac taccagaacg tgccgccagc agggtatccg
    14581 ggtgacggga gtccatgatc actgtaccgt acgcaccgcg cagctgcggg atagcacgca
    14641 gaacagcctc acgcagagtc ccgccttgtt tcagctccca gttcaccaga tgggcaatca
    14701 cttcggtgtc ggtttcagaa acgaaggtat agccacgcgc ttttaactct tcacgcagcg
    14761 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
    14821 gcgcgttagc ttctgaaggt tcaccgtggg tcgcccagcg agtgtgagca ataccggtgc
    14881 cgccatccag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
    14941 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
    15001 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
    15061 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
    15121 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
    15181 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
    15241 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
    15301 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
    15361 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
    15421 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
    15481 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgcccga ctttcgagcc
    15541 tttacccaga cgcgcttttt tcatctcaac gaagttacca acgtgtgcac cttccagcaa
    15601 ctcagcacca ggacgcaaac gagcaaacgg gccaatagta caggccgctg ccagattcgc
    15661 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
    15721 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
    15781 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
    15841 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
    15901 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
    15961 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
    16021 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
    16081 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
    16141 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
    16201 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
    16261 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
    16321 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
    16381 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
    16441 gtgaccgtac accaagtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
    16501 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
    16561 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
    16621 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
    16681 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
    16741 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
    16801 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
    16861 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
    16921 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
    16981 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
    17041 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
    17101 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaacggt cacgttgcca
    17161 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
    17221 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
    17281 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
    17341 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
    17401 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
    17461 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
    17521 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
    17581 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
    17641 catccatacc caagatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
    17701 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
    17761 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
    17821 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
    17881 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
    17941 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
    18001 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
    18061 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
    18121 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
    18181 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
    18241 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
    18301 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
    18361 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
    18421 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
    18481 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
    18541 tacctaccgg gacttcgatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
    18601 cgtcggagga acccattgcg atggtacgca cgataccgcc gccgagctgc tgctgaactt
    18661 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
    18721 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
    18781 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
    18841 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
    18901 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
    18961 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
    19021 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
    19081 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
    19141 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaagc
    19201 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
    19261 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
    19321 gatcattgcg aggtcgcatt gaacgctttt gtcggtccag gtcttcattt ccgccagtag
    19381 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
    19441 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
    19501 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
    19561 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
    19621 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
    19681 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
    19741 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
    19801 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
    19861 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
    19921 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
    19981 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
    20041 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
    20101 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
    20161 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
    20221 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
    20281 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
    20341 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
    20401 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
    20461 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
    20521 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
    20581 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
    20641 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
    20701 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
    20761 ccgatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
    20821 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
    20881 acagcagaga agccgtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
    20941 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
    21001 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
    21061 tcgcgctcaa ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
    21121 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
    21181 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
    21241 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
    21301 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
    21361 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
    21421 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
    21481 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
    21541 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
    21601 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
    21661 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
    21721 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
    21781 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
    21841 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
    21901 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
    21961 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
    22021 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
    22081 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
    22141 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
    22201 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
    22261 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
    22321 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
    22381 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
    22441 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
    22501 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
    22561 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
    22621 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
    22681 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
    22741 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
    22801 cagctcaccg gcatacatgt taccgaacag tcgcaaaccg agtgaaactg gtttggacag
    22861 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
    22921 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
    22981 caggataaat acgcccagtg ccatagacag cgttacgttc acgtccgcag acggaaccac
    23041 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
    23101 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
    23161 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    23221 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    23281 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    23341 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    23401 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    23461 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    23521 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    23581 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    23641 cggcctttcg ctggtgtata cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    23701 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    23761 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    23821 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    23881 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    23941 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    24001 aaatattttt ccagtgcata caattgcgac ttttccgcta accctgttcg atcatgaaaa
    24061 actgtaaata acgcgtaaac actagtgata aagcgtgctt cagatcacat attgcgcatg
    24121 ttcgcgcaca gtatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    24181 agtctaaaaa cagat
//
LOCUS       DAZSBF010000046        23740 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZSBF010000046 DAZSBF010000000
VERSION     DAZSBF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.047"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..3878)
                     /gene="entF"
                     /locus_tag="ACHXWD_001421"
     CDS             complement(<1..3878)
                     /gene="entF"
                     /locus_tag="ACHXWD_001421"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR7620378.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLN"
     gene            complement(3875..4093)
                     /gene="ybdZ"
                     /locus_tag="ACHXWD_001422"
     CDS             complement(3875..4093)
                     /gene="ybdZ"
                     /locus_tag="ACHXWD_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR7620379.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4096..5298)
                     /gene="fes"
                     /locus_tag="ACHXWD_001423"
     CDS             complement(4096..5298)
                     /gene="fes"
                     /locus_tag="ACHXWD_001423"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR7620380.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5541..7781
                     /gene="fepA"
                     /locus_tag="ACHXWD_001424"
     CDS             5541..7781
                     /gene="fepA"
                     /locus_tag="ACHXWD_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR7620381.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7956..8576
                     /gene="entD"
                     /locus_tag="ACHXWD_001425"
     CDS             7956..8576
                     /gene="entD"
                     /locus_tag="ACHXWD_001425"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR7620382.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8698..8850)
                     /gene="hokE"
                     /locus_tag="ACHXWD_001426"
     CDS             complement(8698..8850)
                     /gene="hokE"
                     /locus_tag="ACHXWD_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR7620383.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9201..9353)
                     /gene="hokE"
                     /locus_tag="ACHXWD_001427"
     CDS             complement(9201..9353)
                     /gene="hokE"
                     /locus_tag="ACHXWD_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR7620384.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9322..9590
                     /locus_tag="ACHXWD_001428"
                     /pseudo
     CDS             9322..9590
                     /locus_tag="ACHXWD_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9793..10911
                     /locus_tag="ACHXWD_001429"
     CDS             9793..10911
                     /locus_tag="ACHXWD_001429"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR7620385.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            10977..11225
                     /locus_tag="ACHXWD_001430"
                     /pseudo
     CDS             10977..11225
                     /locus_tag="ACHXWD_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            11290..11658
                     /locus_tag="ACHXWD_001431"
     CDS             11290..11658
                     /locus_tag="ACHXWD_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR7620386.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11752..12405
                     /gene="nfsB"
                     /locus_tag="ACHXWD_001432"
     CDS             11752..12405
                     /gene="nfsB"
                     /locus_tag="ACHXWD_001432"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR7620387.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12513..13760
                     /gene="ybdG"
                     /locus_tag="ACHXWD_001433"
     CDS             12513..13760
                     /gene="ybdG"
                     /locus_tag="ACHXWD_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR7620388.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13828..15204)
                     /gene="pheP"
                     /locus_tag="ACHXWD_001434"
     CDS             complement(13828..15204)
                     /gene="pheP"
                     /locus_tag="ACHXWD_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR7620389.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15306..18449)
                     /gene="cusA"
                     /locus_tag="ACHXWD_001435"
     CDS             complement(15306..18449)
                     /gene="cusA"
                     /locus_tag="ACHXWD_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR7620390.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKARENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18461..19684)
                     /gene="cusB"
                     /locus_tag="ACHXWD_001436"
     CDS             complement(18461..19684)
                     /gene="cusB"
                     /locus_tag="ACHXWD_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR7620391.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19700..20032)
                     /gene="cusF"
                     /locus_tag="ACHXWD_001437"
     CDS             complement(19700..20032)
                     /gene="cusF"
                     /locus_tag="ACHXWD_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR7620392.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20056..21438)
                     /gene="cusC"
                     /locus_tag="ACHXWD_001438"
     CDS             complement(20056..21438)
                     /gene="cusC"
                     /locus_tag="ACHXWD_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR7620393.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21595..22278
                     /gene="cusR"
                     /locus_tag="ACHXWD_001439"
     CDS             21595..22278
                     /gene="cusR"
                     /locus_tag="ACHXWD_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR7620394.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22268..23716
                     /gene="cusS"
                     /locus_tag="ACHXWD_001440"
     CDS             22268..23716
                     /gene="cusS"
                     /locus_tag="ACHXWD_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR7620395.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVSKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5729 a         6483 c         6027 g         5501 t
ORIGIN      
        1 ctgtttagcg ttgcgcgaat aatcggccca atttttacaa acgcccctgg agagataata
       61 tccacatgcg cacaatcctg acgatagata tccagctccg caatccacgg cgaccaggcg
      121 cgttcaggac tcataccttc ctgaagcgtg cgttcagcaa caaacaacgt cgctttaccg
      181 tcaaacggta cgctatgagc agtcgtcagc aggcgcacag catcagcgta gttgccttca
      241 atggtggtaa acaactccgt tgaagtactt ccctgctgtg ccgccaggaa ggcctcgcgc
      301 tcgcggttaa tctccgccag cacttccggg tccagaccat tagcttcttt ttcctgccag
      361 ttttgcgttt ctggcggcca ggtatccagc aagccaagaa atgccacctg ttcgccacgg
      421 gcacgcagtc gcgccgcaat accctgcgcc agcgtaccgc caagggaata ccccagcagg
      481 taataagggc cgtgcggttg ttgctcaagt aacgttgcca gatgcgcttc gcagacttca
      541 tccaggtttg tcgctgtctg catgggtcca tgagggcgcg gcgactgaat gccaataatc
      601 gaccattgtg gatcgagata acgcgagagt acgctaaact gccaggcaaa accggacgcc
      661 ggatggaaac aaaacagcgt cgggccatta ccttcacgca acggcagaat ggtttcgaat
      721 cccatgcgcc gggagctgtc ctcttcacca tcaataatcg ttgccagttt ggcgacggtt
      781 gacgcgacca tcacctgccc cggcgtcacc tgacgagcaa actgtcgact taactgcgct
      841 gccagtttca ttgccagtag cgaatgaccg cccagcgcaa agaaatcagc atcggcatcc
      901 tgcacgtcac aacccagcaa cgacgcgaat gccgcggcga taatcgtttc actgcccgct
      961 ttcggtgcac gtcccgacgc ttgtgtcttc agttcaggca acggtaaggc tttgcgatcc
     1021 agcttgccgt tggcgctaag aggtaactgt ggaagttgca gcagaaccac cggtaccata
     1081 tgtggtggca atgtttcacg aagctgcgcc tgcaatgcgc tggtatccaa cggcaggccc
     1141 gattgcgaca ccagatagcc caccaactga cgcgcatcac caccagtggc tgccgcctgg
     1201 ttaatcacac aggcgtgggt aacggcttgt tcgacatccg gcagcgcctg catcacgcga
     1261 tcgatttcgc ccagttcgat acgctgcccg cgaattttta gctgatcgtc actgcgcccg
     1321 aggtactcca ccgcgccgtt atccagccag cgggcaacgt ctccggtacg gtacatccgt
     1381 tcgccaggag caaaaggatc ggcaataaag cggctggcgg tcagatcggg gcgtccgaga
     1441 tagccctgcg ccagttgaat gccggtaaga tagagatcac ccgccacacc cggcggcacc
     1501 ggatgcatca tcgcatcaag aatacgcagc cccatgttcc acaccggata accaatcggc
     1561 acactgctgc cgcgcacctg tgccagttcc tcgccaaaag ccggatacca gctgacatct
     1621 accgccgctt ccgtcgggcc atacagatta tgtaacggcg caccggttaa ctgttgccat
     1681 tcacggcata aatcagccgg taaggcttca ccactacaga aaacctgttt caacgtcgcg
     1741 caactctggc gagcggtttg cggagtcagc gaggcaacaa atgccgccag catcgacggc
     1801 acaaagtgcg tggtcgttac gccatattcg gcaaagaatt gctgcatagc gagcgggtca
     1861 cggtgcgctt ccggttcagc catcaccagt tttgcccctg cgataaacgg ccagaaaaac
     1921 tcccacaccg agacatcaaa actgcacggc gttttttggg caacgacatc ttcgcctgta
     1981 agcgggtagt gattttgcat ccataacagg cggttaacga tagccgtctg cccgaccatt
     2041 acccctttcg gcctgccggt ggagccagag gtaaagatga tataagccgt gtggtgcggt
     2101 tgtgaaagtt gcagcggcgc actgccctgg ggggtaagcg gggcgttata gcaaaggctt
     2161 gttaaattgg gaacatcgct aaagcgcggc agttgatcgt cggtggtaat taacagcgac
     2221 ggacgcgcat cttccagcat cattttcagg cgatcgtccg gatagccggt atccagcggt
     2281 agccaggccg cgccagcttc aactattgca tggagtgcca gggtcaaaaa gaccgagcgc
     2341 ggtagtgcca ccgccacgct gtcccctggt ttaacgccgc gctcacgcag cagattcgcc
     2401 agcgccacca cctgctcgcg catttcccga tagctgaaca ggtaacgcgc atctgccagc
     2461 gccggagcat ccggtgtttt tgccgcttgt tctgccacca gcgcgctcag cgtggtttct
     2521 ggaatctcaa cctgagtggc gttgatctgc gccagctgcg catactcacc tggcagcata
     2581 atatcgacat cgccgcacaa cagcgccgga tccgcggcaa actgggcaat cagcattttc
     2641 aggcgttcag catgctggat taacgttggc tcatcgtaac gctgtttatt ggcgaggatc
     2701 tcaatactca aatcaccgtg ttcatccggg aacagggcca gttcaaggtc attaaccgga
     2761 ccggttgcca gggtatgggt ttgcgcctga acaccaggaa tatccagttg gtaatcaaat
     2821 accttaatat tgagtaccgg accaaacagc ggttcatcac ctgccgctcg cccgctgtca
     2881 cggacaattt gttcggcatc gtaacgttga tggcgacgca tttttttcag ttgagccgcc
     2941 agtcgggttg ccagttccgg cagcgtttct tgcgccgcaa tgtgaatacc caacggtaaa
     3001 acgttgagca cgggtccgct agccgtcagc gccgccgagc ccagtcgacg cataaagata
     3061 aatccggcgg catagtccat gcgattgcac aatcgcccca gccacaaggc tgccagcgca
     3121 agggctaaat cggtacgctg cacacctgaa agttgcgtag ccagctggcg gaattccccg
     3181 tcggtaaatt ccagtttcag gcgcagaata tctgccgaag cgctgcgccc cggtaaaggt
     3241 gccggagaaa gtgacgcggg cggcggcagt tgacgacgct gttctgccca gaatgccgca
     3301 tcacgctgcc aggcttcgct ttcgcggtat tgctggtact cttccactac atcagcgaaa
     3361 ggcgtaaacg gcgaagcagg cgttggttcg ccacgcagcc atgtgcagta aatattggcg
     3421 atctggcgag taatggccgg gaaactgaag ccatcgacca gcaaatggtg ataacgctga
     3481 taccagtacc agcggttatc cgcaacatgt atcagctgat gaaagaccag tggtttaccg
     3541 ctatcgacgc gcaaatcttg ttgcaaatcc gccagcatta atgcctgcgc agtaccgtgc
     3601 ggatcaatat tggttcgcag gtcgataatt tctggctgtt cgaatatcag cgcatcatcg
     3661 acccactgcc agacttcgcc gttatcttcc gtaaaacgca tccgcaacgt atctgcttgc
     3721 gctagtccgg caaccaccgc gcgggccagt aatggcgcat caacatctcc agttaactca
     3781 acgtaatgcg ccacgctcca ggcggagggt aaatccgaca gtttttctgc catccagatg
     3841 ccgggctgtg cggcgaccaa aggtaaatgc tggctcattg tgcctcctgc aactgggtga
     3901 aattggccgg tgtcagagta cgccagtgga cttccagcca ctgctggcag gacgcctgtg
     3961 actgcggctg acacacaatg tcccagcctg ccgataagag gcattgttgc ggccacagac
     4021 tgaattgccc ctgcgcattg cgcaaaatgt aaaacgctcc ctgcggatca tcgaagggat
     4081 tactgaatgc catactcaac tcctgtcatg gaaaagtggt tgccagaggt cgattaaccc
     4141 ctgcatcaag ccaccgcgcc aacaaagcgc atcatgtccg ccgtcaacct gacgccagaa
     4201 aatggattct tttatagggt gcaattgcgc atacagcgcc tgattggccc gcatgatcat
     4261 cggctcgcga atacccgctt ccagcacaat gcgcagacct tcggcgctaa cttcaccagc
     4321 ttttagcttt tcaagtaaca cgccctcttg ctgcccgtcc cgatgcggcc accagtacga
     4381 tcctgactgg cttaatacac agccaaagcg ttcaggccag tgcagtccgg catacagcgc
     4441 ggacagccca ccaaaactct gcccggcgac cacggtgcga tcggcgcgat cgctaaaagg
     4501 ggcaatagct ttcaccaggg gtaataactc ttgctgtact gccagccaga aatccgcatt
     4561 acacggcagt tcgtgggcgc ggtgcgtggt gtcgatagcg tcgatcaaca catacacggc
     4621 gggaggaagt tgctgacgat gggtcagcga agtcagcaca ggccagacgg gcatactttg
     4681 cgcccaaaat tcgccatcga gcaaaactgc cagcggacgt tcttctgctg ttacatcgcc
     4741 ggtggtaaaa atccatacac gacgtgaatt tttcaaccgt tcacttttcc agataatttc
     4801 tttggctgct atttctggca cttgcggaca atcccatccc ggttgcagag gcgcttgcgg
     4861 catttcgagt gcagaaacag cgtgccctcg cccgcctttc cagctttgtg gattcagcgg
     4921 atcggctatt gcctggggta atagttttcg ccagccttca cgtaattcga ggcgatcggg
     4981 ggatggtgca gaaaaaatgt catcgcgttc ggtgggaata aagcagtagc tgccgcgcca
     5041 gttggcattg agttgtgtcg tccactgcca gacatcagta cctgcaattc gctgcatcga
     5101 ctggggctgg ctgttctgat gatgatcggt cacaccagtg atgtagatcc atacgcgctt
     5161 tatcgtcgag tattcttcag aaccttgggg atcacgccac cagaaagtga cctcaaacat
     5221 ttcgtcattc agacgctgcc attccgggcc atgtttcgac tgccaccagc tctcacttcc
     5281 tacttttaac gccgtcacac cataacccca tgtttactgt gcaatttttc attgattgca
     5341 gaaatatatt gataatatta ttgataacca tttgcatttg caatagcgta atggcgcgcc
     5401 gtgggaagcg cggacattta ttaaccaact gcactgcgtg tctttcagga tcaaaggttt
     5461 tcgcggtagc gggatgcgtc gtgttgagga cgaccatgcc cgacagttgc aattcgtggc
     5521 aaaaatgcag gaataaaaca atgaacaaga agattcattc cctggccttg ttggtcaatc
     5581 tggggattta tggggtagcg caggcacaag agccgaccga tactcctgtt tcacatgacg
     5641 ataccattgt cgttaccgcc gccgagcaga acttacaggc gcctggcgtt tcgaccatca
     5701 ccgcagatga aatccgcaaa aaccccgttg cccgcgatgt gtcggagatc atccgtacca
     5761 tgccaggcgt taacctgacc ggtaactcca ccagtggtca gcgtgggaat aaccgacaga
     5821 ttgatattcg cggtatgggt ccggaaaaca cgctgatttt gattgacggc aagccggtaa
     5881 gcagccgtaa ctcggtgcgt cagggctggc gtggcgagcg cgatacccgt ggtgatacct
     5941 cctgggtgcc acctgaaatg attgaacgta ttgaagttct gcgtggtccg gcagctgcgc
     6001 gttatggcaa cggcgcggcg ggcggcgtgg ttaacatcat taccaaaaaa ggcagcggtg
     6061 agtggcacgg ctcctgggac gcttatttca atgcaccaga acataaagag gaaggtgcca
     6121 ctaaacgcac caacttcagc ctgaccggtc cgctgggcga cgaattcagc ttccgcttgt
     6181 atggcaacct cgacaaaacc caggctgacg cgtgggatat caaccagggc catcagtccg
     6241 cgcgtgccgg aacgtatgcc acgacgttac cagccgggcg cgaaggggtg atcaacaaag
     6301 atattaatgg cgtggtgcgc tgggatttcg cgccattgca atcgctggaa ctggaagcag
     6361 gttacagccg ccagggcaac ctgtatgcgg gcgacaccca gaacaccaac tccgattcct
     6421 atacccgctc gaaatatggc gatgaaacca accgtctgta tcgccagaac tactcgctga
     6481 cctggaacgg cggctgggat aacggagtga ccaccagcaa ctgggtgcag tatgaacaca
     6541 cccgtaactc gcgtattccg gaaggtctgg caggcggtac cgaagggaaa tttaacgaaa
     6601 aagcgacaca ggatttcgtc gatatcgatc ttgatgacgt gatgctgcac agcgaagtta
     6661 acctgccgat tgatttcctc gttaaccaaa cgctgacgct gggtacggag tggaatcagc
     6721 aacggatgaa ggacttgagt tccaataccc aggcgctgac cggggcgaac accggcggcg
     6781 ctattgatgg tgtgagtgcc accgaccgta gcccgtattc aaaagcagaa attttctcgc
     6841 tgtttgccga aaacaacatg gagctgactg acagcaccat cgtaacgccg gggctgcgtt
     6901 tcgatcatca cagtattgtc ggcaataact ggagcccggc gctgaacata tcgcaaggtt
     6961 taggcgatga cttcacgctc aaaatgggca ttgcccgtgc ttataaagca ccgagcctgt
     7021 accagactaa cccgaactac attctctaca gtaaaggtca gggctgctat gccagcgcgg
     7081 gcggctgcta tcagcaaggt aacgatgacc tgaaagcaga aaccagcatc aacaaagaga
     7141 ttggtctgga gttcaaacgc gacggctggc tggcgggcgt cacctggttc cgtaacgatt
     7201 atcgcaataa gattgaagcg ggctatgtgg ctgtagggca aaacgcagtc ggcaccgatc
     7261 tctatcagtg ggataacgtg ccgaaagcag tggttgaagg tctggaagga tcgttaaacg
     7321 taccagttag cgaaatggtg atgtggacca ataacatcac ttatatgctg aagagtgaaa
     7381 acaaaaccac gggcgaccgt ttgtcgatca tcccggagta cacgttgaac tcaacgctga
     7441 gctggcaggc gcgggaagat ttgtcgatgc aaacgacctt cacctggtac ggtaagcagc
     7501 agccgaaaaa atacaactac aaaggtcagc cagcggttgg cccggaaacc aaagaaatca
     7561 gtccttacag cattgtgggt ctgagcgcga cctgggatgt gacgaagaat gtcagtctga
     7621 ccggcggcgt ggacaacctg ttcgacaaac gtttgtggcg tgcgggtaat gcccagacca
     7681 cgggcgatct ggcaggggcc aactatatcg cgggtgccgg ggcgtatacc tataacgagc
     7741 cgggacgtac gtggtacatg agcgtaaaca ctcacttctg atgctaacgt cagattgttg
     7801 acaaagtgcg cgtcgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaat
     7861 cttgccaatt caatatattg caggaccatg taggcctgat aagcgtagcg catcaggcag
     7921 ttttgcgttt gtcatcagtc tcgaatatgg tcgatatgaa aactacgcat acctccctcc
     7981 cctttgccgg acatacgctg cattttgttg agttcgatcc ggcgaatttt tgtgagcagg
     8041 atttactctg gctgccgcac tacgcacaat tgcaacacgc tggacgtaaa cgtaaaacag
     8101 agcatttagc cggacggatc gctgctgttt atgccttacg ggaatatggc tataaatgtg
     8161 tgcccgcaat cggcgagcta cgccaacctg tctggcctgc ggaggtatac ggcagtatta
     8221 gccactgtgg gactacggca ttagccgtgg tatctcgtca accgattggc attgatatag
     8281 aagaaatttt ttctgtacaa accgcaagag aattgacaga caatattatt acaccagcag
     8341 aacacgagcg actcgcggac tgtggtttag ccttttctct ggcgctgaca ctggcatttt
     8401 ccgccaaaga gagcgcattt aaggcatcgg agagacaaac tgaagcaggt tttctggatt
     8461 atcagataat tagctggaat aaacagcagg tcatcattca tcgtgagaat gagatgtttg
     8521 ctgtgcactg gcagataaaa gaaaaaatag tcataactct gtgccaacac gattaattga
     8581 caacatctgg tacgattcgc ccgcagccat cactgactgc gggcgaaagt gtaaagcagg
     8641 tgccttacca ctctgacctg acaaccggat atgcagggat tgctccccgc acaacggcta
     8701 cttcttcggt tcgtaagcga gaacagcctt aaactcaata ttacgttcct ttacggtgaa
     8761 ctcacacagc gagtctccga ccagaagcgt aaatcccagc accgtcaaac acagcactat
     8821 gactgccaca agggcatatt tcgtcagcat gtttatattg cctccatgcg aagaagggac
     8881 taccatccga gttgttgagg ttcggagtgg cagccccagg attgatagaa atatctcctg
     8941 gggcttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca ggcacccgcc
     9001 gttatcttac ccttcctcag caaaatatct gtttatttat acagtaagtc ctgtttcgga
     9061 cttctgttgg caacactcgt tattgctcgt aaaacgaaat cgcccgcagc catcactgac
     9121 cgcgggcgaa agtgtaaagc aggtgcccta ccatcctgac ctgacaaccg gatatgcggg
     9181 gattactccc cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca
     9241 atattacgtt ccttcaccgt aaactcacac agcgagtctc cgaccagaag cgtaaatccc
     9301 agcaccgtca aacacagcac tatgactgcc gcaagggcat atttcgtcag catgtttata
     9361 ttgcctccat gcgaagaagg gactaccatc caaattgttc gggtttggag tggcagcccc
     9421 agggttgata gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa
     9481 ccggaaagct tcaggcaccc gccgttatct tacccttcct caacaaaatg gctgtttatt
     9541 tatacagtaa atcctgtttc ggacttctca ctcctcattt aatcaactga agctgagccc
     9601 gttttttctg catgcctcaa tcgtcaaaat tcttcgccat cagccagctc tgccataact
     9661 tgccacattg aaaaagtagc caaattgctt cagtcaccat catcaaaaaa agaaacattc
     9721 cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa aacgccaaca
     9781 taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac cctcggtatt
     9841 gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga ctcttcaatg
     9901 ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga tatcaccgaa
     9961 agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc cgggcaattt
    10021 tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga aatttgcggc
    10081 ggtggcacac acccgtttca gaaatggcag cgtcaggagg tatgcgataa cgagcgctat
    10141 caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt tggtcagcat
    10201 gtccatgttg gctgcgccag tggcgatgac gccatttatt tgctgcacgg cttgtcacgg
    10261 tttgtgccgc actttatcgc cctttccgcc gcgtcgccat atatgcaagg aacggatacg
    10321 cgttttgcct cctcacgacc gaatattttt tccgcctttc ccgataatgg cccgatgccg
    10381 tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtttgagtta caccacgatg
    10441 atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt tggcacggtg
    10501 gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat ggcgggatta
    10561 attcaggcta ccgcccactg gttactgaca gaacgcccgt tcaaacatca ggagaaagat
    10621 tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atgggctgga aggcgtcatt
    10681 accgatccgc acactggcga tcgtcgaccg ctaacggaag acaccttgcg attgctggaa
    10741 aaaatcgccc cttccgcaca taaaattggt gcatcgagcg cgattgaggc cctgcatcgc
    10801 caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc cgatggtggc
    10861 tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccgatgacta agtagggaat
    10921 tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac aatgttatga
    10981 aacacccttt agaaaccttg actaccgcag caggcatttt gctgatggct ttcctctctt
    11041 gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg gtgaccatgt
    11101 tccatctgat ggatcttagc tagctttaca ctttattgtt ttgtctgtgg tttttagtgc
    11161 tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc tcgctggcgg
    11221 attaaacgtc gattatcgcc gtacttcgcg gcatactttg cttattctcc ttcgcctaaa
    11281 ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctggc ccttgagtta
    11341 ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa aattggcggc
    11401 aaaattttta tgctgtcgtc ggagctgcgc ggcgtcccct ttatcaatct gaagtccgat
    11461 ccacaaaaat ccctgttaaa tcagcagata tacccgagca ttaagccagg gtatcacatg
    11521 aataaaaagc actggatttc ggtgtatccc ggcgaggaaa tctccgaagc gttacttcgc
    11581 gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg cgatcaaaaa
    11641 agagtgcgtc caggctaaag cggaaatcta tagcgcattt ttctcgctta ccatttctcg
    11701 ttgaaccttg taatctgctg gcacgcaaaa ttactttcac atggagtctt tatggatatc
    11761 atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagtaa aaaacttacc
    11821 ccggaacagg ccgagcagat caaaactctc ctgcaataca gcccatccag caccaactcc
    11881 cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt tgccaaatcc
    11941 gctgccggta attatgtgtt caacgaacgt aaaatacttg atgcctcgca cgtcgtggtg
    12001 ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga ccaggaagat
    12061 gctgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg tcgcaagttc
    12121 ttcgctgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc aaaacaggtt
    12181 tatctcaacg tcggtaactt cctgctcggc gtggcggctc tgggtctgga cgcggtaccc
    12241 atcgaaggtt ttgacgccgc catcctcgat gcagaatttg gtctgaaaga gaaaggctac
    12301 accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa cgctacgctg
    12361 ccgaaatctc gtctgccgca aaacatcacc ttaaccgaag tgtaattctc tcttgccggg
    12421 catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt ttcagccgtc
    12481 atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc caggttgaag
    12541 atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc ggtattattt
    12601 ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg accttcgaaa
    12661 aacgagccat cgccagttca cggctttggt tgcaaatcat aacccagaat aaactcttcc
    12721 accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta ttctggctgc
    12781 aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg atcatgatgt
    12841 atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg cagaaattcc
    12901 cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg atcggcgcga
    12961 tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg attctgatca
    13021 gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg attcttggtc
    13081 tggtggccgg tattcagctt tccgcgaacg atatgctgaa actgggcgac tggctggaga
    13141 tgccgaaata cggcgcagat ggcgcggtga tcgatattgg gttaaccacc gtcaaagtgc
    13201 gtaactggga caataccatt accactattc ccacctggtc tctggtttct gactccttta
    13261 aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt atcagtattg
    13321 atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat aaagcgcatt
    13381 tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat cgccagcaag
    13441 gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc tttcgtgcct
    13501 atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc ttaatggtac
    13561 gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt actaacaccg
    13621 tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata tttgcgattg
    13681 tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt cgctctctgg
    13741 cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga gagcggacat
    13801 cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa atgccacaaa
    13861 caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat ccatcgtgca
    13921 catcagcagc aaaatcatgc cgaggaaggc aatgcagaga tagtttccga acggatagag
    13981 cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gctgcacgaa aacgcagatg
    14041 cgccagacag atcataatcc agttcaacag cagcgttgct accaccagcg ccatcagcag
    14101 accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag tgatcgctcc
    14161 ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca aaaacttcgg
    14221 cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa ccccgctgtt
    14281 atacactgac agcgatgcta ccagaatgac gaagttcagc gcagaagcta ccacgttgct
    14341 gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt ccacccacgg
    14401 atagagcgcc agtaaaacca ccagtgaacc gatgtaaaac agcaggatgc gatacaccac
    14461 ctgatttacc gcttttggaa tgcttttatc cggatcgcgc gcttcagcgg cagtaatccc
    14521 aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa tcagcccatt
    14581 ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg ctttctcgcc
    14641 gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga ttgccagcac
    14701 tttaatcaac gcaaaccaga actcggtttc gccatataag cgcacgttca ccaggttaac
    14761 ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat ccgggaacca
    14821 gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca cgaacattac
    14881 ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat aggcaaagtg
    14941 ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc gcataatcag
    15001 gaaagcgatg atcccggcga cgccgtaacc cagcaataca gccggacccg ccatctgaat
    15061 cgccggacca atgccaagaa acagaccagt accaattgcg ccacccagcg caatcagttg
    15121 aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg cagtatcttc
    15181 cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt tgaggggcac
    15241 cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa cggggtatcc
    15301 tgcttttatt tccgtacccg atgtcggtgc agccacatca gcttatacgc cgccgggata
    15361 ataaacagcg acagcaaagg tgcggtgatc atgccgccaa tcatcggtgc ggcaatccgg
    15421 ctcatcacct ctgaaccagc ccccgttccc cacagaatcg gcagcagacc ggcgataatc
    15481 accgccaccg tcatcgcttt cgggcgcacg cgcagtaccg cgccgtgata caacgcctca
    15541 tccagcttct gctcactgaa tgtttgcgga ttattcaacg acggctcggc ctctatggcg
    15601 tgacgtaaat acatcagcat caccacgcca aattcggcgg cgaccccggc aagggcaata
    15661 aaaccggtgc ccgttgccac agaaagatga aagcccatcc accacagcaa ccagatgccg
    15721 cccaccagcg caaacggtac gctgctgata atcagcaacg cttcgcccac ccgacggaat
    15781 gccagataca acagcacgaa gatgatcatc agtgtcatcg gcaccatgag tttcagctta
    15841 tggttggcgc gctccagtag ctcgaactgc ccggagaatg ccacgctggt gcccggtttt
    15901 aactgcactt tttcagctat cgctttttgc aaatcgtgaa ccaccgacac catgtcacga
    15961 tcgcgggcat caatataaat ccagctcgtc gggcgcgcat tctcggtttt cagcatcgac
    16021 ggtccggtag agactttaac gtcggccacg tctgccaggg tgatttgctg cttcatcggc
    16081 gtcaggatcg gtagctggcg cagtgcctgc gggctatcgc gccagctttg cggataacgc
    16141 agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc cccgcccacc
    16201 gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc ggctttttca
    16261 cggttaatct caacgttgat atagcgccca ccttccagac gctcggctag agcagaagcc
    16321 acgcttggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc gatatccgcc
    16381 agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga gagcatatcg
    16441 atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg cacggtgtta
    16501 tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc ctgcggctta
    16561 agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt ttccgctttc
    16621 ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt gtcggttttt
    16681 tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg catatacaac
    16741 aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag cggccagaga
    16801 accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg cagtactttc
    16861 agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact gctttccggc
    16921 ggaattttgc cacggatcca gtaccccatc aggataggga tcactacgat cgccagcagc
    16981 gccgcgcccg ccatcgcata ggttttagtg aacgccaacg ggccaaacag acgcccttcc
    17041 tgcccttcca gggtgaagat cgggataaac gacaacgtga taatcagcag actgataaat
    17101 agcgccggac ccacttcaac agatgcatcg gtgatcacct gccagcgcgt tttattatcc
    17161 agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgtgc attttcgatc
    17221 atgacgatag cggcatcgac catcgccccg acggcaatcg caatgccgcc caacgacata
    17281 atattggcat tcagtccctg gaagtgcatg acaataaaag caatacacaa ccccagcggc
    17341 aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc acagactacc
    17401 gccaccacga taaactcttc cagcaacttg ccgctgaggt tgtcgatggc gcggtcaatg
    17461 agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact acttttcagc
    17521 gtttccagtt tgtccttcac ggaggcgatc acttctcggg cgtttttgcc ggagcgcagg
    17581 atcaccaccc cgcccgccac ttcgccttcg ccattcagtt cggcaatgcc ccggcgcatc
    17641 tccgggccaa tctggacctt cgcaacatca cgcagataaa cgggcacgcc attttctctg
    17701 gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct ggcgcgcacc
    17761 atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt tgaagcatcc
    17821 agcgcgcttt ttacttcggc tagactgatg ccatattgcg ccaggcgctg gggatcgata
    17881 accacctgat actctttcac cacgccgccc accgacgcca cttccgcaac gtcagggatg
    17941 gtttttagct catatttgag aaaccagtcc tgcaatgagc gtaaatcggc cagatcgtgc
    18001 ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt ggcatctggt
    18061 cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag gtactccagc
    18121 acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata cacataagag
    18181 tctccaaact gcgagaaacc gcgcacagtc ttcgcgccag gcaccgacaa catggtggtg
    18241 gttagcggat aagtcacctg attttcaacg atttgcggtg cctgaccggg atagctggtt
    18301 ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt gttaatgatg
    18361 gtccaggtgc cccagatgct cagaaacaac gcgcccatca gcaccaaaaa acggttcgcc
    18421 accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca ttggtagcac
    18481 tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca atcaggaaca
    18541 ggccgctgga aaccaccttt tcaccttccg ccagaccaga gtgtaacgcg gtgacgcctt
    18601 gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg tccgtcggca tcaacggtaa
    18661 tcacccgctg ttcgttgccg gtatcaatca gcgcctgtga cggaatgagc agcatcggtt
    18721 cgctggcggt gttgagttgc aaccaggcgt tcatgcccgg ttttagcgcc tcgtcggcgt
    18781 tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgcca ggtagcagcg
    18841 tccatttgcg gatggtgagt gttttatccg gtcgcgccgg aacggtgagc gtaaactgcg
    18901 aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc cacaccgggt
    18961 ccataccctg aattttcgct accacgttat ctttggcgat attcattccc gcgcgcagat
    19021 caaacgcggt gatcacgcca tcaatgggtg ctttgagcgt aaagcgagtc tggatttttt
    19081 gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc agccgctcaa
    19141 gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa tactcactct
    19201 gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt tgtactttat
    19261 cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc tgcacaatgg
    19321 catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa gtcagcggtc
    19381 cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca
    19441 caccagacgc agaactctct tcatcggcat atttcggcac cagatccata tccataaacg
    19501 gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac cagaataaga
    19561 ttttacgttc tgcggtcgac gttttttctg cgggtggttc agcctttgca aaccaggtaa
    19621 aacctgccgc agaaataata ccgcccgcga tcatgctgcc gataataagc gcgatttttt
    19681 tcatctcatt aaacctgggt tactggctga ctttaatatc ctgtaataaa gaaaggttgc
    19741 cctgctggac aaaattaaac gccactttgt cgccggtttt aatttcactc attttcgtct
    19801 gcggggtgat ggtaaagcgc atagtcatct ccggccagtt cacggcagca atcggatcgt
    19861 gatggatggt gatttttttg ctttccagat caataccctt taccacgcca gtggcgctaa
    19921 taacctgcgg ttgtacttcg ctcatggttt catgatgatg ttcgttagcc tgggcattaa
    19981 agccaataac ggtaaacaga ctgaacattg cgacttgcag tgcttttttc atttattctc
    20041 tcctggagtt aaaaattatt gctgccaacc gccccctaag gcggtataca aagaaatttc
    20101 gttaacctga cgggcataat tcaggtcgag taaagtttgt cgggttgcaa ataaagaacg
    20161 ctcggcatcc agcacttcca gataacttac cgcgccgtgc tgatataatg cccgcgcccg
    20221 ttgcaaagta atttgcagcg acgccagata acgctgctgg gcgctgattt gatcgttcag
    20281 gctttgacgt aatgccagcg catctgccac ttctttaaag gcgttctgga ttttctgttc
    20341 ataattcact accgactgct gctggcgaat ttcggcgata tccagattgg cctggttgcg
    20401 tccggcatta aaaatgggga tctcaatttt aggaataaaa ttccacatcc cgctgctagc
    20461 gttaaataat gaagacagat cgctgctggc ggttgatatt ccgctggtca ggctgataga
    20521 cgggaaaaat gccgcacgcg cagcaccaat attagcatta gccgccatta acgcgtgttc
    20581 agcttccata atatccgggc gctgcaataa gatttgcgac gacaagcccg ccggtaattt
    20641 aacgctttgc aggctgccgc tgtttactgt ctgcgcttgc ggcagtttgc cgtagcttcc
    20701 caataacagt tgcaatgcat tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc
    20761 actgcgggta ctttctatca ccccgcgagc ctgttccagc gccagaacat tgctgctacc
    20821 ggtcaacagt tgtttttcga caaacgcata tgactgctga taattacgca gcgtttcttc
    20881 ggctatttgc aattgcgcat acgccagttg ctgattgaaa tagctttgcg cgacattaga
    20941 aaccagcaga atatgcaccg cgcgctgagc ttcctcagtg gctaaataat tttgtcgctc
    21001 ggcttcgctc atgttcttta agcgaccgaa aaaatcgagg tcaaagctgg cgttaaggcc
    21061 ggtcgaaaac tcccgtgtcg tggcggtatt ccctttaaga ttaccgctcc agctgccgct
    21121 gccctcgcca ttgagctgtg ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc
    21181 ctcctgcact ttcagcgtcg ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat
    21241 cagcgtcttc acctgattat caacaaaaaa ggtgcgccag cccacgttct ggtagttatc
    21301 tgctgcgtta accaagccgt tctggctgag tgagaactgc tgcggcacgg gcattgccgt
    21361 acgctgataa tccggtgcca gtgaacaacc ggttagcgca agggccacac aaaatggcag
    21421 aagtttacaa ggagacatag gctcataatt tctggtgatt ttataccgcc aactttactc
    21481 gccaggctct gattttccgg tgacaggaaa atgacaaaat tgtcattttg ccaataagcg
    21541 attgccatct gatcccgcta ctctagaatt gcccgggcaa catgcggagg aaaaatgaaa
    21601 ctgttgattg tcgaagatga aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa
    21661 gccggttttg tggtcgattt ggccgacaac gggctgaatg gctaccatct ggcgatgacc
    21721 ggtgattatg acctgataat cctcgatatt atgctgccgg acgtgaacgg ctgggatatc
    21781 gtgcgcatgt tgcgctccgc caataaaggg atgccgattc tgttgcttac cgcgcttggc
    21841 accattgaac atcgcgtcaa ggggctggag ttgggggccg atgactatct ggtgaagccg
    21901 ttcgcttttg ctgaactgct ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg
    21961 attatcgaaa gtcagtttca ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc
    22021 acccgcagcg gcacgcgcat cactctgacc agtaaagagt ttactctgct ggagtttttt
    22081 ctccgccatc agggcgaagt gctgccccgc tcgcttatcg cctcgcaggt gtgggacatg
    22141 aatttcgaca gcgacactaa cgccattgat gtggcggtga agcggctacg cggcaaaatc
    22201 gacaacgact ttgagccgaa gctgattcag accgtgcgcg gcgtgggcta tatgcttgag
    22261 gtgccggatg gtcagtaagc catttcagcg cccgttttcg ctggcaaccc gtctgacctt
    22321 ttttatcagc ctggccacca tcgcggcgtt tttcgccttt gcatggatca tgatccactc
    22381 agtgaaagtg cattttgccg agcaggatat taatgattta aaagaaatta gcgccaccct
    22441 tgaacgggta cttaatcatc ctgacgagac gcaagcccga cgcttaatga cgctggaaga
    22501 tatcgtcagt ggttattcca acgtgttgat ttccctggca gatagccacg gcaaaacggt
    22561 gtatcactcc cccggtgcgc cggatatccg cgagtttacg cgtgacgcca tacccgataa
    22621 agacgctcag ggtggcgagg tgtatctcct ttccggcccg acgattatga tgccaggcca
    22681 cggtcacggg catatggaac acagcaactg gcggatgatt aacttgccgg ttggcccgtt
    22741 ggtggacggc aaaccgattt atacgctcta catcgcgctt tcgatcgatt ttcatcttca
    22801 ttacataaat gatttgatga ataaacttat tatgaccgca tctgtcatca gcatcctgat
    22861 cgtctttatc gtgctattgg cggtacataa aggtcacgcg ccgatccgca gcgtcagccg
    22921 tcaaatccag aatatcacct cgaaagatct cgacgttcgc cttgacccgc agaccgagcc
    22981 cattgagctg gaacagctgg tactgtcgtt caaccatatg atcgagcgta ttgaggatgt
    23041 ctttacccgc cagtccaatt tctcagcgga tatcgctcac gaaattcgca cgccgattac
    23101 caatctcatt acgcaaacgg aaatcgccct cagccagtct cgcagccaga aggagctgga
    23161 agatgtgctc tactctaatc tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat
    23221 gctgtttctc gctcaggccg ataacaacca gctaatcccc gaaaagaaaa tgctcaacct
    23281 ggcggatgaa gtcagcaaag tgttcgattt tttcgaggcg ttagcggaag atcgcggcgt
    23341 ggagttgcga tttgttggcg acaaatgtca ggttgcgggc gatccgctga tgctgcgtcg
    23401 ggcgttaagc aacctgcttt ctaatgccct gcgttatacg ccaaccagag agacaattgt
    23461 agtgcgctgt cagacggtcg atcaccaggt gcaagttacc gtcgaaaacc ccggtacgcc
    23521 cattgcgccc gagcacttac cgcgattgtt tgaccgtttc taccgcgttg acccttcccg
    23581 ccagcgaaaa ggcgaaggta gcggcattgg gctggcgata gtgaaatcga ttgttgtcgc
    23641 gcataaaggc acggttgcag taacgtcaga tgcgcggggg acaaggtttg tgattatgct
    23701 gccggagaga gagtgattat taagttcaaa ttcagggcgt
//
LOCUS       DAZSBF010000047        23486 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZSBF010000047 DAZSBF010000000
VERSION     DAZSBF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.048"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            2..688
                     /locus_tag="ACHXWD_001441"
     CDS             2..688
                     /locus_tag="ACHXWD_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR7620396.1"
                     /translation="MLFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDDRD
                     DLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAECC
                     GEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQYFAGYTF
                     GRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQPLADYPTC
                     PETLKDMSDL"
     gene            685..2112
                     /locus_tag="ACHXWD_001442"
     CDS             685..2112
                     /locus_tag="ACHXWD_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR7620397.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2122..2676)
                     /locus_tag="ACHXWD_001443"
     CDS             complement(2122..2676)
                     /locus_tag="ACHXWD_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7620398.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2778..3485
                     /locus_tag="ACHXWD_001444"
     CDS             2778..3485
                     /locus_tag="ACHXWD_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR7620399.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3482..4936
                     /gene="djlC"
                     /locus_tag="ACHXWD_001445"
     CDS             3482..4936
                     /gene="djlC"
                     /locus_tag="ACHXWD_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR7620400.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDDEEEREAFLEA
                     IQAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAK
                     LHRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLC
                     AYWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTR
                     LLWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVV
                     LEAFPKQEMLARLNTRLEGGDTHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKN
                     DEDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(4996..6666)
                     /gene="hscC"
                     /locus_tag="ACHXWD_001446"
     CDS             complement(4996..6666)
                     /gene="hscC"
                     /locus_tag="ACHXWD_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR7620401.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(6758..7693)
                     /gene="rihA"
                     /locus_tag="ACHXWD_001447"
     CDS             complement(6758..7693)
                     /gene="rihA"
                     /locus_tag="ACHXWD_001447"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR7620402.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(7811..8536)
                     /gene="gltL"
                     /locus_tag="ACHXWD_001448"
     CDS             complement(7811..8536)
                     /gene="gltL"
                     /locus_tag="ACHXWD_001448"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR7620403.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(8536..9210)
                     /gene="gltK"
                     /locus_tag="ACHXWD_001449"
     CDS             complement(8536..9210)
                     /gene="gltK"
                     /locus_tag="ACHXWD_001449"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR7620404.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(9210..9950)
                     /gene="gltJ"
                     /locus_tag="ACHXWD_001450"
     CDS             complement(9210..9950)
                     /gene="gltJ"
                     /locus_tag="ACHXWD_001450"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR7620405.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(10120..11028)
                     /gene="gltI"
                     /locus_tag="ACHXWD_001451"
     CDS             complement(10120..11028)
                     /gene="gltI"
                     /locus_tag="ACHXWD_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR7620406.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            11483..13360
                     /locus_tag="ACHXWD_001452"
     CDS             11483..13360
                     /locus_tag="ACHXWD_001452"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR7620407.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(13438..14976)
                     /gene="lnt"
                     /locus_tag="ACHXWD_001453"
     CDS             complement(13438..14976)
                     /gene="lnt"
                     /locus_tag="ACHXWD_001453"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR7620408.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(15001..15879)
                     /gene="corC"
                     /locus_tag="ACHXWD_001454"
     CDS             complement(15001..15879)
                     /gene="corC"
                     /locus_tag="ACHXWD_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR7620409.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(15969..16436)
                     /gene="ybeY"
                     /locus_tag="ACHXWD_001455"
     CDS             complement(15969..16436)
                     /gene="ybeY"
                     /locus_tag="ACHXWD_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR7620410.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(16433..17473)
                     /locus_tag="ACHXWD_001456"
     CDS             complement(16433..17473)
                     /locus_tag="ACHXWD_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR7620411.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(17626..19050)
                     /gene="miaB"
                     /locus_tag="ACHXWD_001457"
     CDS             complement(17626..19050)
                     /gene="miaB"
                     /locus_tag="ACHXWD_001457"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR7620412.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            19196..20371
                     /gene="ubiF"
                     /locus_tag="ACHXWD_001458"
     CDS             19196..20371
                     /gene="ubiF"
                     /locus_tag="ACHXWD_001458"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR7620413.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(20525..20599)
                     /locus_tag="ACHXWD_001459"
     tRNA            complement(20525..20599)
                     /locus_tag="ACHXWD_001459"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20565..20567),aa:Gln,seq:ctg)
     gene            complement(20636..20710)
                     /locus_tag="ACHXWD_001460"
     tRNA            complement(20636..20710)
                     /locus_tag="ACHXWD_001460"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20676..20678),aa:Gln,seq:ctg)
     gene            complement(20759..20835)
                     /locus_tag="ACHXWD_001461"
     tRNA            complement(20759..20835)
                     /locus_tag="ACHXWD_001461"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20799..20801),aa:Met,seq:cat)
     gene            complement(20851..20925)
                     /locus_tag="ACHXWD_001462"
     tRNA            complement(20851..20925)
                     /locus_tag="ACHXWD_001462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20891..20893),aa:Gln,seq:ttg)
     gene            complement(20960..21034)
                     /locus_tag="ACHXWD_001463"
     tRNA            complement(20960..21034)
                     /locus_tag="ACHXWD_001463"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21000..21002),aa:Gln,seq:ttg)
     gene            complement(21058..21142)
                     /locus_tag="ACHXWD_001464"
     tRNA            complement(21058..21142)
                     /locus_tag="ACHXWD_001464"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21106..21108),aa:Leu,seq:tag)
     gene            complement(21151..21227)
                     /locus_tag="ACHXWD_001465"
     tRNA            complement(21151..21227)
                     /locus_tag="ACHXWD_001465"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21191..21193),aa:Met,seq:cat)
     gene            complement(21607..23271)
                     /gene="asnB"
                     /locus_tag="ACHXWD_001466"
     CDS             complement(21607..23271)
                     /gene="asnB"
                     /locus_tag="ACHXWD_001466"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR7620414.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(23225..>23486)
                     /locus_tag="ACHXWD_001467"
     CDS             complement(23225..>23486)
                     /locus_tag="ACHXWD_001467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620415.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPDPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         5734 a         5817 c         6031 g         5904 t
ORIGIN      
        1 aatattgttt gcactttcca ctccgatgga aatacgcaat gaatgctgtt taccttcgca
       61 ttcatccccc aaaatgtatt taggtacatg cttttttgac ctttcttcct catggggaat
      121 tgatgaccgt gatgacctgc tgcgcacaat tcaccgaatg atcgataacg gtcatgccgc
      181 tcggttggca gggttttatc accgctggtt tcgttattcg ccatgtgaat ggcgtgacta
      241 tcttgctgaa ctaaatgagc aaggtcaggc ttatgcgcaa tttgtcgcca gcacagccga
      301 atgctgtggt gagggaggaa ttaaagcatg ggattatgtc cggatgggtt ttttaagtcg
      361 aatgggcgta cttaataact ggttgagtga agaagaaagt ctatggatcc agtcgcgtat
      421 tcatctacgg gctctacgtt attatagtaa ctggcggcaa tactttgccg gttatacctt
      481 tggtcggcaa tattggcagt ctcccgaaga tgatcatctg cctttattac gcgaattctt
      541 agctcgtaaa gaatacgacg attccggcaa tgatatgttt tatcaattat ttgccagtga
      601 tgatgcgtat taccctgcct tgtcctggca accactggct gactacccca catgcccgga
      661 aacgcttaag gatatgagcg acctatgaaa aattgctgga agatcctcga aatagaggaa
      721 acgactgacg tcgatattat ccgccgcgct tatctggcgc tgttactgtc ctttcatcca
      781 gaaaccgatc cgcagggttt taaacaactt cgtcaggcgt atgaggaggc gctccggatt
      841 gcgcagtcgc ctgctaaatc tgtttggcaa ccagaagaat atgaggtagc agaacatgaa
      901 attctgctcg cctttcgcgc gttacttgcc tctgatagcg aacgttttct gccctccgcc
      961 tggcagcgat tcattcagca attaaattat tgctcgatgg aagagattga tgaattacgc
     1021 tggtcgctgt gcacaatcgc catgaacact gcccatttat ccttcgagtg cgtggtgtta
     1081 ttagcagaaa gattgcggtg gttgcaggag gaaaacgtcg gggaaataga cgaagaagaa
     1141 ctggaatcct ttttatatgc cattgcgaag gggaatgttt ttaacttcca gaccattctg
     1201 catctgcccg ttgccgtgca aaatgacacc atcgattttt accaaatgtt cgctcggatt
     1261 tggtcatcgc atccagaatg gctgacattg tatttagcgc aacatcgcgc agtgatcatc
     1321 cccgatgatg caaaactgca cagaaattta ctccgctggt atagcgcagg tcgcctggat
     1381 atccccgaac ttctggatta cgcccggtcg tggcgggaag ctgaacctga taatgaagat
     1441 gcgcgttatt atgaatacgc gcaacgcgtc tattgtggag aaggcgaaag cttgctggca
     1501 gaactttgtg actactggcg cgagtatccc tccacccagg cggatgcttt aatattgcaa
     1561 tggtgccgtc agcatcgggt cgattattac ccgttagtgg tgatgatgat tgaagctcgt
     1621 gaactggtta acgatcaggg taaacagcta ctttatgtcc ccggcgacag cgcccgtacg
     1681 cgttttcatt tatacgaaat actcagcgat gaaaaactct ctgcgctggg gcgttcactg
     1741 gtcgagatgg ttttgcacaa aggacgtaag ccgcggatct cactcacgcg tgatacagaa
     1801 catcccttat ggccattata tttagttgcc aaacaattag tgcaggccag ccaaccgaca
     1861 gaagaatcat taatgccgat tgtgagccgc cttgatgcag aagatcgttg tccactggaa
     1921 gcattaatta ttcgtcgatt attaattcag gcggcgaatt ttaccggtca ggaaaccgtc
     1981 gagccggagc cgcaaccgca gccaatgccc gttgacgatg gcgggctggg ctgtctgggc
     2041 gtcatcaaaa ttattttcta catttttatc tttgccggtt tgataggaaa aatactccat
     2101 ctcttcgggt gacagtcgac gttaatgtgc gggaagtccg gcaagtaatc tttccgcatt
     2161 tttgtaatgt tgtttggcgg ccagttttaa ccatttttga gattcataat ggttaacatt
     2221 aataccagtg ccatttctgt agatcaaacc caaagtatat tgagcatgag catacccttg
     2281 ttcagctgct tccttatacc agagaattgc tttacgggta tcgggtttga ctcccaggcc
     2341 tttcaggtag atccacccca gtttgctttg tgcgcgcgga ttttcctgtt ctgctgattg
     2401 ctcataccag tatctcgcct gtgtataatc cagtggttta ccgtcgctac catgaagata
     2461 aaattcgcca aggcgatatt gcgatgttga gtgaccatga atcgcggaaa gctccgtggc
     2521 tgccgtggtt aacggtagac tgcctggtga tcttaaataa tcaaaggcat aattcaccat
     2581 ggctttatca cgcaacgtcg ttttcttggg ttggctaata caggtaattg ctgcaactat
     2641 aaaaattgcg aatatataca ttgatttttt attcatgtgt gaatgcttcg attttctttt
     2701 tattttgaaa gcgtcaagtt tatactcatc aataataaaa aagtagtcag ttaatgcact
     2761 taagggatta ttaaaaaatg aataaggaag aacaatatct tctttttgcg ctttctgcgc
     2821 ccatggaaat acttaatcag agttgcaaac ccgcgcatga ctcgccaaaa atgtatactg
     2881 ggataaaaga attcgatctc tcatcatcct ggggaataaa taatcgtgat gatttaattc
     2941 agactattta tcagatgact gatgatggtc atgccaatga tttagctggc ttatatctga
     3001 catggcaacg ctcttcccct gaagagagga aagcattaat tgctggtggc tcggagagag
     3061 ggttgattta tacccaattc gttgcccaaa ccgctatgtg ctgtggagaa gggggaatca
     3121 aagcctggga ttatgttcga atggggtttc tgtcacgggt cggtgtgctc aacaactggc
     3181 tgacggaaga agagagttta tggctgcaat cgcgcgtcta tgcacgagct caccattact
     3241 atcacagctg gatgcactat ttttctgcct attcactagg gcgtctctac tggcaatctt
     3301 ctcagtgcga agacaacgct tcgctacgcg aggcgctaac cctgtacaaa tacgatagcg
     3361 ccgggagtcg gatgttcgaa gagctggcag cgggaagcga ccgtttctat gccacgcttc
     3421 cctggcaacc attaactgtc cagcctgaat gcccggtaac gcttaaggat gtgagcgacc
     3481 tatgaaaaca tgttggcaaa tcctcgaaat tgaaagcacg acgcaaatag acattatccg
     3541 ccaggcttat cttgctcgct taccgttgtg tcatcccgaa accgatccgc aggggtttaa
     3601 agcattacgc caggcctatg aagaggcgct gcgactggcg gtaaatcctg tcgaggaagc
     3661 cgatgatgaa gaaaaagatg ccaccgctga acatgaaata ctacgtgcat tcaggacatt
     3721 actggattca gaaagtgatc gttttcagcc ctccgcctgg cagaaattta ttcagcaatt
     3781 aaatacctgg aacatggagg atgtcgatca attacgctgg ccgctgtgtg caatcgctat
     3841 agaagcgcga tatctttcat taaattgtgc ttctttgttg gcagagcgtt tgaactggca
     3901 ttcgtttaat gacagcgaag gaatggatga tgaggaagaa agggaggctt ttcttgaggc
     3961 cattcaggct ggtgattgtt tcgatttcct tagccttctg gaatatcccg tcgcgttaca
     4021 gaaccagact gttgaatatt acttcgcgct ggaacgttgc tgccgttacc atcctgacta
     4081 tgtcactgcg tttttggcga tggaaggacc gtggttcatt cctgatgatg caaagttaca
     4141 tcgcaaactg ttgcgctggt acagctcggt gcaaacaggt atggcggaac tcattcctgt
     4201 agccaaacag tggcaagcgg aagaaccaga aagcgaagat gcccggtatt accagtgtgc
     4261 acaacgtttg tactgcggcg agggggaaag cctgcttgcc gatctctgcg cgtactggga
     4321 aagttaccca tctacacaag ctgataattt gttgttgcag tggagcaagc gtcattgccc
     4381 ggattatttc gcgttattag tgatggttat cgaagcgcga agcatggtag atgcgcaagg
     4441 taaaccgttg aaatatgttc ctggtgagag cgcccggacg cggctgttat gggcggagat
     4501 tttacatagc ggaaaattat cgccgttagg tcaatcgttt attgagtcgt tattcttcaa
     4561 gcgcaaagca tgggcgtggt ggaaatcgag agtcggtagc gagacagagc aagattcacc
     4621 gttactggat ttgtatcggg tagcggaaca ggttgtgctg gaagcgttcc cgaaacaaga
     4681 gatgctggcc cgtcttaata caaggctgga aggtggagat actcatccat tagaggccat
     4741 tatcacccag atgctgttga cgaaagtgaa actcgagccg gaggatgaag atgtcgatga
     4801 gccaacacct gaaaaccatg aagaaaaaaa tgatgaggat gaaaaaccac agagcattac
     4861 cagtattatc aaaatcagct taacggtgct gatgataggt tatgcgctcg gaaaaatcgc
     4921 gatgttgttt agctgaggat gtgttttttg cgccgatgcc ggatgcagcg tttatgccgc
     4981 atccggcagc cgtgtttaag ggatctcaat agccaaataa tcgctcgccc gtcgacgaac
     5041 ttcatcgacg cgggccatat cgtttgactg caacgccgca tcaaaatcgg tgatcacccg
     5101 gccaatctct tctcgctcgt cacccagcgc ccgcgcccac aactcttcca gtttggcctt
     5161 aaaggtgcgg ttgattagca tatcgcgcgg ataaattttc aatgcggata accgcgtccg
     5221 actctcttca atctgctgcg cgctcaatgt taccgggcta tgattaatga ctctggactt
     5281 aacgctgcca tcttccagaa gcacgtcaac ttcaagcaag ccgttgatat cataactaaa
     5341 gcgaatatca atcgactgat aagccccggt tttcttcaac ggcacatcga aggattcaac
     5401 cagaatgtta tttttaactt tgtggttttc tccctgatag acgttaaccg taatagaatc
     5461 ctgttccggg tgcatggtcg agtaagtttc tacacgcgac acgggtactg tggtgtttcg
     5521 ttcaataatt ggcgagaaaa tgccggaaac gccctggcgg ttaacttcaa cgcccaacga
     5581 gtaagggcaa atatcagtga ggatcacctc ttcaatatct tcactgcgta agcggcaggc
     5641 ggcctggatt gctgcgccca gcgcgacaat ggtgctcggg tcgtaacttt gatacggtaa
     5701 tttgccaaac agacgcacgg cgattcgctg caccagcggc atttgtgacg cgccgccaac
     5761 cagcaccaga ctgtcgattt gactcggctt cagacgtgca tcgcgcaacg cctgttcaat
     5821 cggcactcgc aagcgattga gcagcggcaa ccacaaatct tccagttcgt tctcgtaaaa
     5881 ttcgcactcc cgcgtttcat cctggtactg ccagcgaatg tgcaatggcg attgattgct
     5941 acattttgcc gcttccacac aggcgtacaa ggctgccagt tcactctcgt taagcgtggt
     6001 cctggcgaca tccgcgcgct ttaaaacgtc atcgaccaac atatgggtaa aatcttcgcc
     6061 accgaggaag ttatcgccag cggaggcgtg aacttcaatc accggcgtgg cgtattcgag
     6121 caccgtgacg tcaaacgtgc cgccaccgag atcaaaaacc agcgagcggg tattttgttg
     6181 cgtatgcagg ccatatgcca tcgcagctgc tgtgggttca ttaattaagc gtaccgcatt
     6241 taacccggct aactccgctg ctaaacgggt atgcttgcgt tgttcatcgc tgaaataagc
     6301 cggaacggag atcaccacat ctttaatcgg acgttgcaga aattcttcgg catcttcttt
     6361 taatgagcgt aataccaaag aggacagttc tggcgcgtta aatgtgtcgc tgcctaaccg
     6421 ccagttggta ttactgccca ttgcacgttt aaataacgct gccgttttat ccggatgcga
     6481 agtacgccgt gatacagccg gttttccgac taaaatatga ttattttcat ccatgctaat
     6541 tatggatggt gttaaatatt caccgaactt atttggaatt aattgcgcgg caccgtcttt
     6601 ccagacggca attaaactat tggtagtgcc gagatcgata ccaatggcga gttctgcatt
     6661 atccattgca tttatatctt gaaagaaaaa gagataaaca gataaacaga ttaagaccac
     6721 ccaggtaacg cgtggtgatc ttaatcaatg acgtgtgtta agcgtaaaat ttcagacgat
     6781 cagccagtaa atcaacaaag ccctgacgat caacatcgac cattacagtg gcattcggtt
     6841 tattgccagt cagataataa taatcaacaa ccgtcatacc ctggctatat ttcccctgtg
     6901 tttccacgcc aacccagcgc tcaacagtgg taaacaactc gggtttcaac agccaggcga
     6961 tggtgcatgg gtcatgcagc ggtgcaccga caaagcccca tttttcgtct ttatgatatt
     7021 cgaggaagaa atccagcagt tcggcaacaa tggttgaaac agggttgcca atcgcgcgga
     7081 aacgctcggt gtcttcaacg tggatttgtg ctttgtgagt aacatccaga ccggccatca
     7141 ccactgggat ccctgactgg aagacaattt ctgccgcttc cgggtcaacg taaatattaa
     7201 attcagccgc aggcgtccag ttaccaagcc ccattgcgcc gcccataatc acgatacggg
     7261 caattttgct atgcagttcc ggatggctat tgagcagcaa ggcaacgtta gtttgcggtc
     7321 cggtagacac aatcgtgaca ggttccgcac tttcgcgcag cgttttcgcc atcagctcta
     7381 ccgccgtaca gttttgcggt gcgaacgtcg gttccggtaa tgccgggcca tccagaccgc
     7441 tttcgccgtg cacattgtcc gcgataatca actcacgcat taacggtttt accgcgccgc
     7501 ctgctaccgg aatatcggtg cgattaagca aggtcagcat acgcagaaca ttgcgtaagg
     7561 ttttttctgg tgtctggttt ccggcggaag acgtaattgc tttgacatca agctctggtg
     7621 aggcgagggc gagaactatt gcgatagcgt cgtcatgacc tgggtcgcaa tctaacagaa
     7681 ttggcagtgc cattgttgct ccttgttgtg tgcttctttg cgacaagggt aacgccagga
     7741 tgtaacagat acgaggggcg aaacgataaa gcgtgagatg gcgcgcactt gggtatgcgc
     7801 gccagagtga ttaatgcagg attttcgcga ggaagtcttt cgcgcggtcc gatttcggat
     7861 catcgaagaa agcgtctttc ggcgagtctt cgacaatttt accctcgtcc ataaagatca
     7921 cccgattcgc cactttgcgg gcaaagccca tttcgtgggt caccaccatc atggtcattc
     7981 cttcgttcgc cagttccacc atcacgtcca gcacttcgtt gatcatctcc ggatccagcg
     8041 ccgatgtcgg ttcgtcgaac agcatcgcaa taggatccat acacaatgcg cgagcgattg
     8101 ccacacgctg ctgctgaccg ccggaaagct gcgccggaaa tttattggca tgtgcggaaa
     8161 gtcccacgcg ctccagcaat ttcaatgctt tttcacgagc gggcgcttta tcgcgtttaa
     8221 gcactttcac ctgcgccagg gtcaggtttt cgataatcga cagatgaggg aacagctcga
     8281 aatgctggaa taccatcccg acgcgggaac gcagctttgc cagatcggtt ttcttgtcat
     8341 taaccacgat accatcgacg gtgatttcac cttgctgcac tggttcgagg ccgttgacgg
     8401 ttttaatcag cgttgatttg ccggaaccag acgggccgca aaccaccacc acttcgcctt
     8461 ttttcacttc ggttgagcag tcggtcagca cctgaaagtg accataccat tttgaaacat
     8521 ttttcagggt aatcattatg ctgtccttct tttcaagtag ctgaccaaca acgacgcgct
     8581 aagactaata acgaaataaa caaatccggc aaacaggatc atctcaacct gcgtaccatc
     8641 acgctcacca atggttgagg cggtacggaa gaaatcggcc agagataaca catacaccag
     8701 tgaggtatcc tggaacagta cgatgccctg agtgagcaga agcggcacca tcgcgcggaa
     8761 cgcctgcggc agaataatca gtttcatcga ctgccagtga gtcattccca acgccagcgc
     8821 ggcgctcgac tgaccacgag aaatactttg aataccagca cggataatct ctgaatagta
     8881 ggccgcttca aacatcgaaa acgccaccat cgccgaaatt aaacggatat cattttttgg
     8941 tgataatccc agcacgtttt gcagaaaacc tggcacgatc aggtaaaacc acagcaaaac
     9001 cataactaaa ggaatcgagc ggaatacgtt aacgtaggct ttggcaaacc acgctacggg
     9061 cgcaaagctg gacaagcgca tcaccgccag catggtgccc cacaaaatac caatcacgac
     9121 tgccgtgacg gtgattttca gcgtgatcac cagcccgtcg agcagatatg gcagggaagg
     9181 gacaatggaa ctccagtcaa actcgtacat tatttgcccc ccatgttgcc aggcaggcga
     9241 actttacgtt caaccagcgt cattaccagc atgataaccg cgttaatcaa cacatacgcc
     9301 agcgtaatag cggtaaacga ctcccaggcg tgggctgagt aatcaagcaa tttacccgcc
     9361 tgcgccgcca tatccaccag accaatagtc gaggcgatgg cggagttttt caccaggttc
     9421 atcatctcgg aagtcatcgg cgggacgata acgcgataag cattaggcag cagtacgtat
     9481 cgataagcct gcggtagcgt caggcccatc gccagcgcgg catttttttg cccacgcggc
     9541 aacgactgaa tggcggcgcg tacctgttcg caaacacggg cggcggtaaa cagccccagg
     9601 cagagcatgg atgaaaggaa aaactgaata ttgggatcca gctcggcctt aaaccacatg
     9661 ccgattttct ccggcaacag ctccgggatc accagatacc aggtaaagaa ttgcacaatc
     9721 aacggcacgt tgcggaacag ttcgacatac aacgtaccca gaccggagag aaaacggttt
     9781 ggaacggtac gtaaaatgcc gaaaaatgaa ccgacgagga aagcgataat ccaggcgcag
     9841 atcgacaaag cgatcgtcac ctgaaaacca ctccagatcc aaccaagata ggtggtgttg
     9901 ccgaacgggg cttgttgtaa aaaaataccc cagttccagt ctatagacat aaatctactc
     9961 cagaaaaaag agggtagcag cgttaactgc taccctcgaa gattgttacc cagcgtattg
    10021 cggttttcag gcccgatggg gaacgaccat caggcgtata gtctgtccgt gctacgtaac
    10081 aatcgagagg gctggaattt ccgcccctgg ttcttgtaat tagttcagtg ccttgtcatt
    10141 cggttctttg aacagtgctt tcatttcgtc tgacagttcg aaattcatgt tcaggttttt
    10201 cggcggaatg ggatttttga accatttatc aaaccatttt tccgcttcac cggaggtctg
    10261 cacctgcgcg gtggtgtcat ccatcagctt tttgaactgc ggatcgtctt tacgcaacat
    10321 gcaaccgtag gcttcctgag actgcggctt gccgacgatt tcccagttgt ctggtttctt
    10381 cgctttcgca cgttcaccgg ccagcagggc atcatccatc ataaaggcaa cggcacgacc
    10441 gctttccagg gtgcggaaag agtcactgtg atctttggcg ctgatgatgc gcatattcat
    10501 tttttgctct tcattcagtt tgttgagcaa aacttcagag gtagtgccgg aagtgacgac
    10561 tacggctttg tctttcaggt cggcaaaatc tttgatatcg ccgccctttt tggtcagcag
    10621 gcgcgtaccg accacgaaaa tagtatcaga gaaagccgcc tgtttttggc gttcgacgtt
    10681 gttggtggta gaaccacatt caaaatcgaa agtgccgttt tgcagcagtg gaatacggtt
    10741 ttgtgaggta atcggaatca gttttacctg caagtccggt ttgttgagtt tctttttcac
    10801 tgcttcaaca atggcgttgg agtaatcctg cgagtaaccc accacttttt gctgattgtc
    10861 gtaataagag aaaggtactg aagattcacg gtgaccgacg acaatcacac cgtttttggc
    10921 gattttgtcc agcgtactgc ctgctgccgg ggcggcgtca tctgcctgtg ccagtcctgc
    10981 ggaaagcgcc agggcgagga ttgctgtggc aggtttacgt aattgcatat ccaactcctt
    11041 tatcttctgc gttaagaacg catggatacc cgttgtgagt gtttgtgttg ttatcgtctg
    11101 caactttatt gtgcagtgtt gtgcctgtta ggtaacattt agtttggcta aatgtaaaga
    11161 tattgctgtt ttattgtttg tttttgcgag atgcgccgca ccattccgaa gcaaaattct
    11221 taaaccgcac ctttttggtg ctaccgctga tttgtggtgg tgcaacctgg ttgcgtcgcg
    11281 gctgatttca tcgcttacac agtaaataaa gcaacttaca tgccaaaaaa tacttagggg
    11341 cgaattaatt acgattgtaa ttttaggctg aaaaagtcat atttcggaca gaattatctt
    11401 ttcggcatgg gacgtttgcc gggatgataa ctgcacacgg gaaaaatgct ttttacctgt
    11461 cttttcaagg aagaaacacc ttatgtctgc atcttcggtt aagccgttaa atgttcaatt
    11521 acccgcaata acccttatcc tttttgcact ctgtattggg atattttgtt atctcgcaca
    11581 atggatgagt tatgaagagg tcgatcaatc cgcacttatc catctcggcg caaacgttgc
    11641 gcctcttact ttgtcgggtg aaccctggcg cttattgagc agtatctttc tgcacagtag
    11701 tgtttctcat ctgctgatga atatgtttgc attcctggtc gtgggaggcg tggcggaaca
    11761 gattctgggg aaatggcgac tcctgattac ctggttattc tccggcgtct ttggtgggct
    11821 catcagcgcc tgttatgcgt tacgcgaaag tgagcagata gtcatcagcg ttggggcatc
    11881 cggggcgatt ctgggaatag ctggtgcagc gatagcaaca cagtttgctt caggtacggg
    11941 cacataccat aaaaaccagc ggcgagtatt tcctctgttg ggtatggtgg cgctgacact
    12001 gttgtacggt gcccggcaaa caggaataga taacgcttgc cacattggcg gcctgattgc
    12061 gggtggcgcg ttgggttggc tgagcgcgcg tttatctggg caaaaccgac tcgttacgga
    12121 aggcgggatt attgttgcgg gcagtcttct tctgaccggg gctatctggc ttgcgcagca
    12181 gcagatggat gagtcagttt tacaggtcag gcaaagcctg cgtgaagcgt tttatccaca
    12241 ggagattgaa caagagcgac ggcaaaaaaa gcagcagtta gcggaggaac gcaacgccct
    12301 cagggaaaca ttatccgctc cggtaagtcg tgaacaggcc agtggtgatt tgctcgctga
    12361 gattgccgat atccatgata tggcgatcag tcgggatggt aatatgttgt atgccgcaat
    12421 tgaaaacacg aacagcattg ttgttttcga cctcggacaa aagaaaatcc tgcatacctt
    12481 tacagcgccc atagcgaaag aaaagtcagt caaacattgt ggtggctgta aagatcaggg
    12541 cgtcagatcg ctggcattaa gcccggatga aaagttgatt tatgcgactt catttgaagc
    12601 gaatgcgtta tcggtcatta acgtggcgac aggggagatt attcagtcga ttaccaccgg
    12661 tgcacatcct gacagcctta tcctctcgcg tgatggcaca aaagcctggg tgatgaatcg
    12721 caccagtaat agtgtgccag cgattgatct ggtgacttat cagcatgtgg cggatatccc
    12781 gctggagaaa tacgacggga cggggacgag cggtaaacca ggcgcctggg ttatggcact
    12841 ttccccggat gaaagaacat tactggttcc gggagcaggc agaggtaaca tcgtgcggat
    12901 caataccatc acgcatcaga aagaagactt tcccgcaggt gatgcgcgtg gaacgatatc
    12961 ggcgatgcgt tttcgacctg aaaacggcga ggttattttt gccgatagtc aggggatttc
    13021 acgtataagc gtaggggatc aacaagccag cattatgacg caatggtgta gcaggagcgt
    13081 ttattccgtt gagggtatta gcccggacgg tcaatattta gcgttggtgt catatggcct
    13141 gcaaggttat gtcatcctgc tcaatattaa tgccgggcag attattggcg tttatcctgc
    13201 cagctacgtt aatcaccttc gtttttcagc ggatggtaga aaaatatttg tcatggcgaa
    13261 gaacgggttg atccaaatgg acaggacgcg ctcgcttgat ccgcaggcaa ttattcgtca
    13321 tccccaatat ggcaatgtgg cttgtatccc tgaaccgtaa gaaaaagggg catgataacc
    13381 atgccccaaa gctcaatgag aaagtacgtt ttgctcatgc aggatacatc aggcacatca
    13441 acgtttacgt gctcgcagac tcatcaacac agcggcaaaa ccaaacagcg ccgtcagcac
    13501 ccacagtggc cagttgccgg tacgtgcata cggtgtgagt ccggtggtcg gtgtcacatt
    13561 agtggtcaac acctcgcggg tgaactgcgg gatcatcgcc tgaacctccc cctgcgggcc
    13621 aatcaccgcc gtaatgccgt tgttggtgct gcgcaacagt gggcgcgcca gctccagcgc
    13681 acgcattcgc gccatctgga agtgctgcca tggaccaata gacttaccaa accacgcatc
    13741 gttggagata gtcagcagat agtcggtatc cgggcggaag ttatcgcgca cttgctcgcc
    13801 gagaatgatc tcgtagcaaa tagccgcagt aagctcaata ccatttaccg acagcggcgg
    13861 ctggatatat ggcccacggc tgaacgacga catcggcaga tcaaagaacg gtgctaacgg
    13921 acgcagaatc gactccagcg ggacaaactc gccaaacggc actagatggt ttttgttata
    13981 gcgatcggct gattcgtagc tgtatggtgc acctttaccc agtgtgatga tggtgttgta
    14041 ggtatcgtag cggttctgct tattgagacg cgcgtcgaca atcccggtta ccagcgagct
    14101 acctttatcg cgcaactcac cgtccagtgc tttgaggaac ggttgctgat taatttccag
    14161 atcggttatc gccgactccg gccagataat caacgatgat ttgcccatca gcggagccgt
    14221 tgcgttgtag taaatcttca gcgtattaag aagctgatct ccatcccatt tcagtgattg
    14281 cggaatatcg ccctgaacca tcgaaacctg aatggttttc tccggttgcg gggtaaacca
    14341 ttggatgtaa cgcagcggga agggtagggc aaacagcacg acggccacca ccagcggacg
    14401 ccagttgcgt ttgaccaacg ccagtgccag caggccacta accatcatca gcaggaagtt
    14461 aatggcttcc acgcccatta tcggtgccag cccttttaac gggccatcaa tctggctata
    14521 gccgaactgt aaccacggga agcctgtcag tacccaaccg cgcagaaact cggtcacttg
    14581 ccagagagca ggggcggcaa tcgccacgcg cagccaggtg gttttcggcc acagacgcga
    14641 cagcacgcca gcaaacagtc cggtatacag cgacaaatac gccgccagca gcaccaccag
    14701 gaagatgtta accgggccag gcattccgcc aaaggtcgcg atgctgacat agacccagtt
    14761 aataccgctg ccaaagaggc caaatcccca gcaaaagcca atagcggcag actggagtgg
    14821 acggcggtta aaggtcaacg cctgaagccc catcaacgaa ataatcgccg caggccagac
    14881 gtcgtaagga gagaaggcca gcgttccgca ggcaccgaat aataacgcca gcagcaggcg
    14941 aatgcgctgg cgttcaatta atgaggcaaa agccatgtag ttatctatcc agtttcggtt
    15001 ttattcatcc agcttcggct ggggtgagtc atccgggatt ttgacatgaa cctgaataat
    15061 acgccgactg tcggccatcg ccactttgaa ctggtaaccg tcgatgtcga tagtttcgcc
    15121 acgcgccgga agatgcccaa atgcctgcat caccagacca ccgatagtgt caacctcttc
    15181 atcgctaaag tgggtgccga acgcttcgtt gaagtcttca atggaagcca gtgcacgcac
    15241 ggtccaggta tgacgactca gctgacggaa gtcgatatca tcttcttcgt catactcgtc
    15301 ttcaatctca ccaacaatca gttccaggat gtcttcaatg gtcaccagac cggaaacccc
    15361 accgaattcg tcaatgacga tcgccatgtg gtaacgctga gagcgaaact ctttcagcat
    15421 ccggtctacg cgcttacttt caggaacgac aaccgcctga cgtaacactt tgtccatgct
    15481 gaaggcttca gcatcgctgc gcataaacgg cagcaagtct ttcgccatca gaatcccttc
    15541 aatgtgatct ttgtcttcgc taatcaccgg gaaacgtgag tgggcggact cgatgatgac
    15601 atcaaggcat tcgtccagcg tctggttgcg tttcagggta atcatctggg agcgggggat
    15661 catgatgtcg cgaacgcgtt ggtctgcgat gtccatcacc ccttcgagca tatcgcgcgt
    15721 atcttcgtcg ataaggtcgt tctgcccgga atcacggatc agcgccagca gttcgtcacg
    15781 gtttttcggt tcaccgtgga aaagttggct gagtaacagg gagaaaaatc ccttcttgtt
    15841 gcttatcgtg tcactactgt gtgaattgtc gtcgctcatg gcgtcgtatg ggttctcatg
    15901 ttagttaatc aaaacgtcgt cgttaatcac caacggcggg gacgtctgcc agtcaaatgc
    15961 ctggcaaatt attctttctc ggcaatgtac ggatcctcat agcccagagc aagcataatc
    16021 tctgtttcga gggcttccat ttcttctgct tcgtcatctt cgatgtgatc gtaacctaac
    16081 aaatgcagac tgccgtgcac caccatatgc gcccagtgcg cctccagtgg tttgccttgc
    16141 tcctgagctt ccttctcaac cacctgacgg cagataacca gatcgcccag tagcgacatt
    16201 tccatgccag gcggcacttc aaacgggaag gagagcacgt tggtcggctt atccttaccg
    16261 cgataggtca gattcagact gtggctttcg gcggtatcga ccacgcgaat agtcacttcc
    16321 gattcttcct gaaactgcgg gatcaccgca ttcagccatg tttgaaactg gctctcttcc
    16381 ggtaacccgg aattatcttc acatgccagt tgtaaatcga ggatcacctg actcattttt
    16441 gttcctgttc ttcgcgcttg cgttctgccg ccagcgccgc ttttcgtttt tgttcggctt
    16501 cttcccaggc ttcataggcg ttaacgatac gcgccaccac agggtgacga accacgtctt
    16561 cgctgtggaa gaagttaaag ctgatctctt cgacatcggc cagcacttcg atggcgtgac
    16621 gtaagcctga tttagtatta cgcggcaggt cgatctgtgt gacgtcgccg gtgataaccg
    16681 cttttgagtt aaaaccgata cgggtcagga acatcttcat ctgttcgatg gtggtgttct
    16741 ggctctcatc gagaatgata aacgcgtcgt tcagcgtacg accacgcata taggccagcg
    16801 gcgcgacttc aataacgttg cgctcaatca gtttctcaac tttctcaaag cccagcattt
    16861 caaacagcgc gtcgtacagt gggcgcagat acgggtctac tttctggctt aaatcgccag
    16921 gcaggaagcc cagtttctca ccggcttcga ccgccggacg agtcagcaga atacggcgaa
    16981 tctcctgacg ctccagggca tcaactgccg cagccactgc caggtaggtt ttacccgtac
    17041 ccgccgggcc aacgccgaag gtaatgtcat ggtcgagaat attggcgatg tactgcgcct
    17101 ggtttggcgt gcgcggctta attacgccgc gtttggtttt gatattgacc gctttgccgt
    17161 actccggcac gctctccgca ctttgctcca gtactcgcgc ttctttaatc gcaaggtgga
    17221 tctgttccgg ttcgatatcc tgaatctgac cgcgcatcgg ggctgtatcg acatacaggc
    17281 tacgcagaat gtctgccgca gcggtgacgc aaatcggacg gccggtcagt ttaaagtggt
    17341 tgtcgcggcg attgatctcg atgccgagac ggcgttcgag ctgcttgatg ttgtcatcaa
    17401 acgggccgca caggctcaac agacgcgcgt tgtctgctgg ctccagggtg atttcgcgag
    17461 tgtctatgtt caaaccgttc ctcttatctg tatgccgccg gaagctgaac attcaccggc
    17521 ctataaggaa attattcacg ccacaggaaa aaggcgcaag cattgcgata aagatgggga
    17581 taaagagaga aaaaacaagg cccaccggaa cggcgggcct gagaattacg gctgataata
    17641 acccacgcca aggtcgtttt ctttgcgggt acgggcaatc accgattccg gtgtttctgc
    17701 cacgcgcaga cccatttcat cttcagtacg caccacttta ccgcgcagag agttcgggta
    17761 gacgtcggta atttctacat cgacgaattt accgatcata tccggtgtgc cctcgaagtt
    17821 gaccacgcgg ttattttcgg tacgcccgga aagctccatg atgctcttac gcgaagtccc
    17881 ttctaccaga atacgctggg tggtgccgag catccggcgg ctccatgcca tcgcttgctg
    17941 attgatacgt tcttgcagaa tatacagacg ttgcttcttc tcttcttccg gaacatcatc
    18001 aaccatatcg gcggctggtg tacccggacg tgcagagaag ataaagctgt agctcatgtc
    18061 gaaattgacg tcggcaatca gcttcatcgt tttctcgaag tcttcggtgg tttcgccagg
    18121 gaagccaaca atgaagtcag aactgatctg aatatccgga cgcgccgcac gcagtttacg
    18181 gatgatcgct ttgtactcca gcgccgtgtg agtacggccc atcaggttca gaatgcgatc
    18241 ggaaccgctt tgtactggca gatgcaggaa gctcaccagc tccggcgtgt cgcgatacac
    18301 ttcgatgata tcgtcggtga attcgatcgg atggctggtg gtaaagcgaa tacgatcgat
    18361 cccgtcgatc gcagcaacca gacgcagcag atcggcaaac gatccggtgg agccgtcgta
    18421 gttttcacca cgccaggcgt tcacgttctg accgagcagg ttgacttcac gcacgccctg
    18481 agccgcaagc tgggcaatct caaacagaat atcgtcagac ggacggctta cctcttcacc
    18541 acgggtgtaa ggcaccacgc agtaggtgca atatttattg cagccttcca tgatggagac
    18601 aaacgcggtc ggcccttcgg cgcgcggttc cggtagacgg tcaaacttct cgatttccgg
    18661 gaagctgata tctacaaccg ggctgcggtc gccgcgcacg gagttgatca tctccggcag
    18721 acggtgcagc gtttgcggcc caaaaataat atcgacatag tgggcgcgct ggcgaatgtg
    18781 ctcgccttct tgcgatgcca cgcagccacc gacgccgata atcaggtctg gattcttctc
    18841 ttttaacagt ttccagcgac ccaactgatg gaagactttt tcctgagcct tctcgcggat
    18901 tgagcaggtg ttcagcagca gcacatccgc ttcttccgcc acgtcggtca gttgatagcc
    18961 gtgggtggca tccagcagat cggccatctt cgatgaatcg tactcgttca tctgacagcc
    19021 ccaggtttta atatggagtt ttttggtcat cgacttgctc ttgcgaaata gtagccagga
    19081 atgcagggcg tatagtgtaa tgctttgctg ccgttgtgac cagtatgagc gttatcagcc
    19141 cttgggggta aaaatcctgt aaacttaaag catattgcta acaggatgat tgaccatgac
    19201 aaatcaacca acggaaattg ccattgtcgg cggaggaatg gtcggcggcg cgctggcgct
    19261 ggggctggca cagcacggat tttcggtaac ggtgatcgag cacgcagaac ctgcaccgtt
    19321 tgtcgctgat agccaaccgg acgtgcggat ctcggcgatc agcgccgcat cggtatcatt
    19381 gcttaaaggg ttaggggtct gggatgcagt acaggctatg cgttgccatc cttaccgcag
    19441 actggaaacg tgggagtggg aaacggcgca tgtggtgttt gacgccgctg aacttaagct
    19501 accgttgctt ggctatatgg tggaaaacac cgtcctgcaa caggcgctgt ggcaggcgct
    19561 ggaagcgcat ccgaaagtaa cgttacgtgt gccaggctcg ctgattgcat tacatcgaga
    19621 taatgatctt caggagctgg aactgaaggg cggtgaaacg atccgcgcga aactggtgat
    19681 tggtgccgac ggcgcaaatt ctcaggtgcg gcagatggcg ggaattggcg ttcatgcctg
    19741 gcagtatgcg cagtcgtgca tgttgattag cgtacagtgt gagaacgatc ccggcgacag
    19801 cacctggcag caatttaccc cggacggacc gcgtgcgttt ctgccgttgt ttgataactg
    19861 ggcatcgctg gtgtggtatg actctccggc gcgtattcgc cagttgcaga atatgaatat
    19921 ggcgcaattc caggcggaaa tcgcgaagca tttcccgtcg cgtctgggtt acgttacacc
    19981 gcttgccgct ggtgcgtttc cgctgacgcg ccgacatgcg ttgcagtatg tgcagccggg
    20041 gcttgcgctg gtgggcgatg ccgcgcatac catccatccg ctggcggggc agggggtgaa
    20101 tcttggttat cgtgatgtcg atgccctgat tgatgttctg gtgaatgccc gcagctacgg
    20161 tgaagcgtgg gccagttatc ctgtcctcaa gcgttaccag atgcggcgca tggcggataa
    20221 cttcattatg caaagtggta tggatctgtt ttatgccgga ttcagtaata atctgccgcc
    20281 gctgcgtttt gtgcgtaatc tcgggttaat ggcggcagag cgtgctggcg tgttgaaacg
    20341 tcaggcgctg aaatatgcgt tagggttgta gccttacaac attgccggat gcgtgccaac
    20401 cgtaggtcgg ataagacgcg gcagcgtcgc atccgacatt gaaggataag acgtgtcaac
    20461 atcgcattcg acattgaatg aacgcagaaa agcaaaaagc tcgccgaagc gagctttttt
    20521 aatgtggctg gggtacgagg attcgaacct cggaatgccg gaatcagaat ccggtgcctt
    20581 accgcttggc gataccccaa ctggtgcact tacgatgtaa gcgtcttgaa taaattggct
    20641 ggggtacgag gattcgaacc tcggaatgcc ggaatcagaa tccggtgcct taccgcttgg
    20701 cgatacccca acaaattggt ttttgaattt gccgaacgta ttcgacacat tcagaatttg
    20761 gtggctacga cgggattcga acctgtgacc ccatcattat gagtgatgtg ctctaaccaa
    20821 ctgagctacg tagccagatt gtttcttcga tggctggggt acctggattc gaaccaggga
    20881 atgccggtat caaaaaccgg tgccttaccg cttggcgata ccccaataac cggtcggtga
    20941 accgcttact cgaagaagat ggctggggta cctggattcg aaccagggaa tgccggtatc
    21001 aaaaaccggt gccttaccgc ttggcgatac cccatccgta caacgctttc tggtgaatgg
    21061 tgcgggaggc gagacttgaa ctcgcacacc ttgcggcgcc agaacctaaa tctggtgcgt
    21121 ctaccaattt cgccactccc gcaaaaaaga tggtggctac gacgggattc gaacctgtga
    21181 ccccatcatt atgagtgatg tgctctaacc aactgagcta cgtagccatc tttttttcgc
    21241 gataccttat cggcgttgcg gggcgcatta tgcgtataga gccttgcagc gtcaacctct
    21301 ttttcaagga aaattgctcg aaagtgactg tttggttagg ttgcgaacag cgtggcgcta
    21361 tattcgtcaa ttattgttta ctttgtgttt tttccccacc ctacagccat tcttttgtca
    21421 tacaggatga aattcggaat tcaacaatgg tggtgggaaa ttaatctatg aaatactggc
    21481 ctacagtgat gagttgtcaa acagtgatgt ggcaaacccg gaacatttcc ttactgcata
    21541 tcagaatcaa caagcacctc aataactgaa acagccccgg atttcaccgg ggctgtttcg
    21601 catttcttac ttatacgccg actggtgaac gccaaccgca cgaccagacg gatcgtccat
    21661 tttcttgaac gcctcatccc actcaatcgc tttagcggaa gaacacgcga cggacgggcc
    21721 gcccggcacg cactcagcgg cgctcggaag cgggaacagt tcttcaaaga tctcccggta
    21781 cagatacgct tcttttgagg ttggcgtgtt gtacgggaag cggaagcggg cagtttccag
    21841 ttgctgatca gaaacctgct gcgccgccac ttctttcagg gtgtcgatcc aactgtaacc
    21901 gacgccatcg gagaactgct ctttctgccg ccaggccacg cttgcgggca ggtatgactc
    21961 aaaacattca cgcaggatgt gtttttccat tttgccgtta ccgcacattt tatcctgcgg
    22021 gttaatgcgc atcgccacat caaggaattt tttgtcgagg aacggaacgc gtgcttccac
    22081 gccccaggct gacatcgctt tgttggcgcg cgcgcagtca tacatatgca gggccagcag
    22141 tttacgcacc gtctcttcat gcagttcttt agcgttgggc gctttatgga agtaaagata
    22201 accgccaaac acttcatcag aaccttcacc ggacagcacc attttaatgc ccatcgcctt
    22261 gatcttacgc gacattaaat acatcggtgt tgaagcgcga atagtggtca catcataagt
    22321 ttcgatgtgg taaatcacgt cgcggatggc atccagacct tcctgtacag tgaagtgaat
    22381 ttcgtgatgc accgtgccca gatggtttgc cacttcctgg gctgctttaa gatccggtga
    22441 acccggcaga cctacagcaa aggagtgtaa ctggggccac caggcttcag agcgttcctg
    22501 atcttccacg cgacgggctg cgtatttctt ggtgatagcg gaaataattg aggaatccag
    22561 accaccagaa agcagcacac cgtaaggcac atcagacatc agatggcttt taacggaatc
    22621 ttccagagcc tgacgcagct cgtttttgtc ggtcacgtta tctttcactg catcaaagtc
    22681 gaaccagtcg cgatgataat aagaacggat ttcgccgtcc tggctccaca aatagctccc
    22741 cgccgggaac tctttaatcg tgcggcaaac tggcaccagc gatttcattt ctgaggccac
    22801 atacagctga ccgtgttcgt cataccccat atacagtggg atgatcccca gatggtcgcg
    22861 accaatcagg taggcatctt tttcgctgtc gtacagggca aaggcaaaca tgccctgcaa
    22921 gtcgtcgaga aattccggcc ctttttcctg atacagcgcg aggatcactt cacagtcaga
    22981 ccctgtctgg aactgataac gatcgccata ttcagcgcgc aatgcctggt ggttgtagat
    23041 ttcaccgttt accgccagta cgtgggtttt ttgttggttg tagagaggtt gcgcccccgc
    23101 gttaacgtca acaattgaca gacgttcgtg agcgagaatg gcgttatcgc tggcataaat
    23161 accggaccag tccgggccac gatgacgcat caggcgtgac agctcgagtg ctttcttacg
    23221 ctgctcaact gcgtctgttt tgatatcgaa tacgccaaaa attgaacaca taaccttctc
    23281 cgttaacctg gtatttgttg cttgttgtgt ttgcttgttt aaaaaaatgc cgcaaagcag
    23341 cactgtgcgc aagcgatttg gcggtgaaaa aataaaaaac gtaatggtga ttgtcgattg
    23401 gtgaaaaagg gtctggtgtg aggatgtatt tattgatgaa tcgataattt ttagcgggtt
    23461 ttattgaatg gttatatttt acgggg
//
LOCUS       DAZSBF010000048        23461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZSBF010000048 DAZSBF010000000
VERSION     DAZSBF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.049"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHXWD_001468"
     CDS             complement(<1..673)
                     /gene="focB"
                     /locus_tag="ACHXWD_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR7620416.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHXWD_001469"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="ACHXWD_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR7620417.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHXWD_001470"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="ACHXWD_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR7620418.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHXWD_001471"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="ACHXWD_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR7620419.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHXWD_001472"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="ACHXWD_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR7620420.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHXWD_001473"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="ACHXWD_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR7620421.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHXWD_001474"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="ACHXWD_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR7620422.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHXWD_001475"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="ACHXWD_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR7620423.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHXWD_001476"
                     /pseudo
     CDS             complement(8399..9837)
                     /gene="hyfD"
                     /locus_tag="ACHXWD_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHXWD_001477"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="ACHXWD_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR7620424.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHXWD_001478"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="ACHXWD_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR7620425.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHXWD_001479"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="ACHXWD_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR7620426.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHXWD_001480"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="ACHXWD_001480"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR7620427.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="ACHXWD_001481"
     CDS             complement(14170..14742)
                     /locus_tag="ACHXWD_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR7620428.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="ACHXWD_001482"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="ACHXWD_001482"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR7620429.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="ACHXWD_001483"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="ACHXWD_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR7620430.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="ACHXWD_001484"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="ACHXWD_001484"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR7620431.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="ACHXWD_001485"
     CDS             17911..18759
                     /locus_tag="ACHXWD_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR7620432.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHXWD_001486"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="ACHXWD_001486"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR7620433.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHXWD_001487"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="ACHXWD_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR7620434.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="ACHXWD_001488"
     CDS             complement(21671..21871)
                     /locus_tag="ACHXWD_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR7620435.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHXWD_001489"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="ACHXWD_001489"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR7620436.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="ACHXWD_001490"
     CDS             complement(23030..23386)
                     /locus_tag="ACHXWD_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR7620437.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5875 a         6230 c         6275 g         5081 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tagccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 a
//
LOCUS       DAZSBF010000049        23314 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZSBF010000049 DAZSBF010000000
VERSION     DAZSBF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.050"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(274..1071)
                     /locus_tag="ACHXWD_001491"
     CDS             complement(274..1071)
                     /locus_tag="ACHXWD_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7620438.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1081..1632)
                     /gene="ybcL"
                     /locus_tag="ACHXWD_001492"
     CDS             complement(1081..1632)
                     /gene="ybcL"
                     /locus_tag="ACHXWD_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR7620439.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1801..2133)
                     /gene="emrE"
                     /locus_tag="ACHXWD_001493"
     CDS             complement(1801..2133)
                     /gene="emrE"
                     /locus_tag="ACHXWD_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR7620440.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            2314..2442
                     /locus_tag="ACHXWD_001494"
                     /pseudo
     CDS             2314..2442
                     /locus_tag="ACHXWD_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2467..2781)
                     /gene="fliE"
                     /locus_tag="ACHXWD_001495"
     CDS             complement(2467..2781)
                     /gene="fliE"
                     /locus_tag="ACHXWD_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR7620441.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2997..4655
                     /gene="fliF"
                     /locus_tag="ACHXWD_001496"
     CDS             2997..4655
                     /gene="fliF"
                     /locus_tag="ACHXWD_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR7620442.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4648..5643
                     /gene="fliG"
                     /locus_tag="ACHXWD_001497"
     CDS             4648..5643
                     /gene="fliG"
                     /locus_tag="ACHXWD_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR7620443.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5636..6322
                     /gene="fliH"
                     /locus_tag="ACHXWD_001498"
     CDS             5636..6322
                     /gene="fliH"
                     /locus_tag="ACHXWD_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR7620444.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6322..7695
                     /gene="fliI"
                     /locus_tag="ACHXWD_001499"
     CDS             6322..7695
                     /gene="fliI"
                     /locus_tag="ACHXWD_001499"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR7620445.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7714..8157
                     /gene="fliJ"
                     /locus_tag="ACHXWD_001500"
     CDS             7714..8157
                     /gene="fliJ"
                     /locus_tag="ACHXWD_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR7620446.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8154..9281
                     /locus_tag="ACHXWD_001501"
     CDS             8154..9281
                     /locus_tag="ACHXWD_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR7620447.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9386..9850
                     /gene="fliL"
                     /locus_tag="ACHXWD_001502"
     CDS             9386..9850
                     /gene="fliL"
                     /locus_tag="ACHXWD_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR7620448.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9855..10859
                     /gene="fliM"
                     /locus_tag="ACHXWD_001503"
     CDS             9855..10859
                     /gene="fliM"
                     /locus_tag="ACHXWD_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR7620449.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10856..11269
                     /gene="fliN"
                     /locus_tag="ACHXWD_001504"
     CDS             10856..11269
                     /gene="fliN"
                     /locus_tag="ACHXWD_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR7620450.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11272..11637
                     /gene="fliO"
                     /locus_tag="ACHXWD_001505"
     CDS             11272..11637
                     /gene="fliO"
                     /locus_tag="ACHXWD_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR7620451.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11637..12374
                     /gene="fliP"
                     /locus_tag="ACHXWD_001506"
     CDS             11637..12374
                     /gene="fliP"
                     /locus_tag="ACHXWD_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR7620452.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12384..12653
                     /gene="fliQ"
                     /locus_tag="ACHXWD_001507"
     CDS             12384..12653
                     /gene="fliQ"
                     /locus_tag="ACHXWD_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR7620453.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12662..13447
                     /gene="fliR"
                     /locus_tag="ACHXWD_001508"
     CDS             12662..13447
                     /gene="fliR"
                     /locus_tag="ACHXWD_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR7620454.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13732..14355
                     /gene="rcsA"
                     /locus_tag="ACHXWD_001509"
     CDS             13732..14355
                     /gene="rcsA"
                     /locus_tag="ACHXWD_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR7620455.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14399..14587)
                     /gene="dsrB"
                     /locus_tag="ACHXWD_001510"
     CDS             complement(14399..14587)
                     /gene="dsrB"
                     /locus_tag="ACHXWD_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR7620456.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14750..14977
                     /gene="yodD"
                     /locus_tag="ACHXWD_001511"
     CDS             14750..14977
                     /gene="yodD"
                     /locus_tag="ACHXWD_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR7620457.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15275..16090
                     /locus_tag="ACHXWD_001512"
     CDS             15275..16090
                     /locus_tag="ACHXWD_001512"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR7620458.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16087..17781)
                     /gene="dgcQ"
                     /locus_tag="ACHXWD_001513"
     CDS             complement(16087..17781)
                     /gene="dgcQ"
                     /locus_tag="ACHXWD_001513"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR7620459.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17952..18134)
                     /locus_tag="ACHXWD_001514"
     CDS             complement(17952..18134)
                     /locus_tag="ACHXWD_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR7620460.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18213..19130)
                     /locus_tag="ACHXWD_001515"
     CDS             complement(18213..19130)
                     /locus_tag="ACHXWD_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR7620461.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19303..20224
                     /gene="yedA"
                     /locus_tag="ACHXWD_001516"
                     /pseudo
     CDS             19303..20224
                     /gene="yedA"
                     /locus_tag="ACHXWD_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20213..20683)
                     /gene="vsr"
                     /locus_tag="ACHXWD_001517"
     CDS             complement(20213..20683)
                     /gene="vsr"
                     /locus_tag="ACHXWD_001517"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR7620462.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20664..22082)
                     /gene="dcm"
                     /locus_tag="ACHXWD_001518"
     CDS             complement(20664..22082)
                     /gene="dcm"
                     /locus_tag="ACHXWD_001518"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR7620463.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22149..22805)
                     /locus_tag="ACHXWD_001519"
     CDS             complement(22149..22805)
                     /locus_tag="ACHXWD_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR7620464.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(22845..23126)
                     /gene="drpB"
                     /locus_tag="ACHXWD_001520"
     CDS             complement(22845..23126)
                     /gene="drpB"
                     /locus_tag="ACHXWD_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR7620465.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5772 a         6074 c         5839 g         5629 t
ORIGIN      
        1 atatctaatg tcataagcat ccctttaatt atcccctcaa cctcttgtaa ctttcccact
       61 cttgaactcc acatagtcca aacgccggat gcaaaaaaat cacacgtgca tttctgaaat
      121 tgtgatggag gaagatagta tcaattatat gtgcttataa acgtccccga ttagcgcaga
      181 agtcaccgga ttgttcaggc tccaataggt tgagtatgcc gtatttattt tccaaaataa
      241 aaatcgatgc tgttgctcct tcataaaata cttttactct ctggacgctt ttcttagctc
      301 tcttggtgtt atcttaaaac gattatggaa cctttcagta aaacgagaag gacacttgta
      361 accattttcc ctggcaatct cgctgatggg ttttactgtc gtttgtatag cagacaacgc
      421 attatttaac ctcacgtcgt ccaatatact ttgaaaactt actccctcgc ttgccagacg
      481 gcgatgcaat gtggaaacag aaatgtagag atatctcgct accttgtttg ctgtccattt
      541 tgttccaggt tcggatagca ttaggttata acaacgactt attaatgatt gtttactatc
      601 tgataaaagt aaacaattaa catgatgtgc tcctaatgaa agtagaacgc ccattgctaa
      661 gtgttcctga atttgcgttg agaagcctcg ggaaatagat gtttttagtt gctcccaaca
      721 atatattaac tcggaattct gaggtaagca gaaacctgtc gtgttacgaa tttgatcggt
      781 tatcgcataa agattttgga acttttcaat tagatcaatg ggataataaa gcatttctgc
      841 aagataaaga cctgcttctg ggtaattcgc aatataaaat tcatatccac agggaaataa
      901 tattatctga ttattatcaa cagttagact atgcgtctcc caattgataa ctttttttcc
      961 ctgacggata cgacatgaag ctggcataag aggcttaacc ctgtgaatct catgatgttt
     1021 atgcatccgt atttcttcaa tctttaggtt agtcctatct tttgccagca tactcttatc
     1081 ctactttacc tcataaacgg gtattatctc agcggttgca attttattgg cattaagcat
     1141 ataaccaact aacgctccgc tggaattaga atccacagga atcttatcag tttttagagc
     1201 ccatatttta aactggtaat gatggggttt atctcctttg ggaggacatg ctccaccaaa
     1261 tcccgcatag ccaaaatcat ttcggccttg gacagcacca gtcggaagtt ttgtaccatc
     1321 acgtcttcct gcatcagcgg gcaaatatgt taccgttgct ggaatattag caacagtcca
     1381 gtgccaccat ccgctgcctg taggcgcatc tggatcatac acagttacgg caaagctttt
     1441 ggtgccttct ggagcaccag accaggttaa tgagggcgat gtattacccc cctcacaccc
     1501 aactccagaa aatacatgag acgttgtaag ttgctctcct gtttttattt cattactagt
     1561 gacctgaaat gctgcggcct gcgcagaaaa tgttatgaat gccaatacac ttgaaacgat
     1621 aagttttttc ataaaaacct ctttgttgtg acctatcgtt attttatttg atgtgcattg
     1681 atctcattat gcaaaaaggc gcaatgatcg cgcaaatgca atcataatta taaagttgcc
     1741 ccgaacatta gccataatat gcacaaatca gattcctgtt atctcaagga tgattatttt
     1801 ttaatgcggt gcgcttcgtg acaacaagtt aatgactaac acaccggcac aaatcaacat
     1861 catgcctatg atggctggca ggtccagtcg ttggccaaaa aatccccagg aaagcaaact
     1921 aatcagaacg ataccgactc ctgaccagat agcataagca atccctgtag gtatataagc
     1981 cagtgtctga gctaataacc aaaatgatgc acaatagcaa ataattgtgc caacagaggg
     2041 ccataaccgt gtaaacccct ctgaaaactt cattaaggtt gtaccaatga cctctgcaag
     2101 tattgcgcca ccaagataaa tataagggtt catagcatat tctttcctgt tcatactgga
     2161 gagaattgta ctacagtttg aacacaactc accgttttca tcctggtgta tgtgtatcca
     2221 gggggctctg gcgtggcaag agataaaggc aggaacaata atgatcagaa ggaaccccag
     2281 gctaacaaaa gagttcactc ttcctatgag caaagcgaag acaaaacatg ttatcgcgtt
     2341 tacggatcgt ggatggcaga aaactttcag gatcaggtat ccattttaaa ccagaaattg
     2401 agtgagtttg ccccatccat gccccacgcg gtgagatcgg atgtaattaa caacaggtta
     2461 caaaacctac acctgcatgg acatcacttc ctgataagcc gccaccagct tattacgcac
     2521 ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca catcgtttaa
     2581 cgccacgccg ggttcaccga gagtgaattt ttctgcctgc gtgcgggcgg ctgtttgtgt
     2641 atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg tcggttgtgg
     2701 cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt aactggctga taaccccttc
     2761 aatcccctgt atcgctgaca ttttcatctc ccggataatt tctggtagca aagcttacca
     2821 gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa aacgggttta ttggcggata
     2881 gaaaaaaacg aaagcacaaa taatggcagc gtcaattttt cgagtttgct gacccgggag
     2941 tgagtcttgt tccactttgc caataacgcc gtccataatc agccacgagg tgcgcgatga
     3001 atgcgactgc agcccagaca aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga
     3061 aaattccatt gattgttgcc ggttccgcgg cagtggcggt catggtcgca ctgatcctgt
     3121 gggcgaaagc ccccgactac cgcacattat tcagcaatct ttccgatcag gatggtggcg
     3181 caattgtcag ccaactgacg caaatgaata ttccttactg cttcagcgaa gccagcggcg
     3241 ctattgaagt tccggcagat aaagttcacg aactgcgtct gcgcctggca caacaaggtt
     3301 tgcccaaagg cggcgcggtc ggtttcgaac tgcttgatca ggaaaagttt ggtatcagcc
     3361 agttcagcga acaggtgaat tatcagcggg cgctggaagg cgagctttct cgtaccatcg
     3421 aaactatcgg cccggtaaaa ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat
     3481 tcgtccgtga acaaaaatcc ccttctgcat cggtgacggt aaatctgtta cccggccgcg
     3541 cactcgatga agggcaaatt agcgccattg tgcacctggt ttccagcgcc gttgctggtc
     3601 tgccgccggg aaacgtcacg ctggtggatc agggcggaca tctgttaacc cagtccaata
     3661 ccagcgggcg cgatcttaat gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta
     3721 ttcagcggcg tattgaagca atcctgtcgc ctattgttgg taacggtaat attcacgccc
     3781 aggtcacggc gcaactggac ttcgccagta aagaacaaac ggaagaacag tatcgcccta
     3841 acggtgatga atctcatgcg gcgcttcgtt cacgccagct taatgagagc gagcaaagcg
     3901 gttccggtta tccgggcggc gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata
     3961 acgcgccaat cagcacgcct ccggcaaatc aaaataaccg ccagcagcag gcgagcacca
     4021 ccagcaatag cgggccgcgt agcacacagc ggaatgaaac cagtaactac gaagtcgatc
     4081 gcaccattcg tcataccaaa atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag
     4141 tggtgaatta caaaaccttg ccagacggta aaccattgcc tctcagcaac gaacagatga
     4201 agcaaattga agatctgacc cgcgaagcga tgggcttttc tgaaaaacgc ggtgactcgc
     4261 tcaatgtcgt taactcgccg ttcaatagca gtgacgaaag cggcggagaa ctgccgttct
     4321 ggcaacagca agcgtttatc gatcagttgc tggctgccgg tcgctggttg ctggtactgc
     4381 tggtggcgtg gctgctgtgg cggaaagcgg tacgcccgca gttaacacgt cgcgccgagg
     4441 cgatgaaagc tgtacagcaa caggcgcagg cccgtgagga agtggaagat gcggtggaag
     4501 tccgcctgag caaagacgaa caactccaac aacgacgcgc taaccaacgt ctgggggcag
     4561 aagtcatgag ccagcgtatc cgtgaaatgt ctgataacga tccgcgcgtg gtggcgctgg
     4621 tcattcgcca gtggataaat aacgatcatg agtaacctga caggcaccga taaaagcgtc
     4681 atcctgctga tgaccattgg cgaagaccgg gcggcagagg tgttcaagca cctctcccag
     4741 cgcgaagtgc aaaccctgag cgctgcaatg gcgaacgtca cgcagatctc caacaagcag
     4801 ctaacctatg tgctggcgga gtttgagcaa gaagctgaac agtttgccgc actgaatatc
     4861 aacgccaacg attatctgcg ttcggtattg gtcaaagctc tgggtgagga acgtgccgcc
     4921 agcctgctgg aagatattct cgaaactcgc gataccgcca gcggtattga aacgctcaac
     4981 tttatggagc cgcagagtgc cgccgatctg attcgcgatg agcatccgca aattatcgcc
     5041 accattctgg tgcatctgaa gcgcgcgcag gccgccgata ttctggcgtt gttcgatgaa
     5101 cgtctgcgcc acgacgtgat gttgcgtatc gccacctttg gcggcgtgca gccagccgcg
     5161 ctggcggaac tgaccgaagt actgaatggc ttgctcgacg gtcagaatct caagcgcagc
     5221 aaaatgggcg gcgtgagaac ggcagccgaa attatcaacc tgatgaaaac tcagcaggaa
     5281 gaagccgtta ttaccgccgt gcgcgaattc gacggcgagc tggcgcaaaa aattatcgac
     5341 gagatgttcc tgttcgaaaa tctggtggat gtcgacgatc gcagcattca gcgtctgttg
     5401 caggaagtgg attccgaatc gctgttgatc gcgctgaaag gggccgagca gccactgcgc
     5461 gagaaattcc tgcgcaatat gtcgcagcgt gccgccgata ttctgcgcga cgatctcgcc
     5521 aaccgtggtc cggtgcgtct gtcgcaggtg gaaaacgaac agaaagcgat tctgctgatt
     5581 gtgcgccgcc ttgccgaaac tggcgagatg gtaattggca gcggcgagga tacctatgtc
     5641 tgataatctg ccgtggaaaa cctggacgcc ggacgatctc gcgccaccac aggcagagtt
     5701 tgtgcccatg gtcgagccgg aagaaaccat cattgaagag gccgaaccta gccttgagca
     5761 gcaactggcg caactgcaaa tgcaggccca tgagcaaggt tatcaggcgg ggattgccga
     5821 aggtcgccag caaggtcatg agcagggcta tcaggaagga ctggcccagg ggctggagca
     5881 aggtctggca gaggcgaagt ctcaacaagc gccaattcat gcccggatgc agcaactggt
     5941 cagcgaattt caaactaccc ttgatgcgct tgatagtgtg attgcttcgc gcctgatgca
     6001 gatggcgctg gaggcggcac gtcaggtgat cggtcagacg ccaacagtgg ataactcggc
     6061 actgatcaaa cagatccaac agttgttgca gcaagaaccg ttattcagcg gtaaaccaca
     6121 gctgcgcgtg cacccggatg atctgcaacg tgtggatgat atgctcggcg caaccttaag
     6181 tttgcatggc tggcgcttgc ggggcgatcc cacactccat cctggcggct gtaaagtctc
     6241 cgccgatgaa ggcgatctcg acgccagtgt cgccactcgc tggcaagaac tctgccgtct
     6301 ggcagcacca ggagtggtgt aatgaccacg cgcctgactc gctggctaac cacgctggat
     6361 aactttgaag ccaaaatggc gcagttgcct gcggtacgtc gctatgggcg attaacccgc
     6421 gctaccgggc tggtgctgga agccaccgga ttacaattgc cgctcggcgc aacctgtgtc
     6481 attgaacgcc agaacggcag cgaaacgcac gaagtagaaa gcgaagtcgt tggctttaac
     6541 ggtcaacggc tgtttttaat gccgctggag gaagtggaag gtgtcctgcc cggcgcgcgg
     6601 gtttatgcca aaaacatttc ggctgaagga ctgcaaagcg gcaagcagtt gccgctcggt
     6661 ccggcgttat taggtcgcgt tctggacggc agcggtaagc cgctcgatgg cctgccctcc
     6721 cccgatacga cggaaaccgg tgcgctgatt accccgccat ttaacccgtt gcaacgtaca
     6781 ccgattgaac atgtgctgga caccggcgtg cgcccaatca atgccctgct taccgttggg
     6841 cgtgggcagc gtatggggct gtttgccggg tccggcgttg gtaaaagtgt gctgctgggg
     6901 atgatggcac gttacacccg cgccgatgtc attgtcgtgg gtttgattgg tgaacgtggg
     6961 cgcgaagtaa aagattttat tgagaatatc ctcggtgctg aagggcgtgc acgctccgtg
     7021 gtgattgccg ctccggcgga tgtctctccg cttctgcgaa tgcaaggtgc cgcctatgcc
     7081 acgcgcattg ccgaagattt tcgcgatcgt ggtcagcatg tgttgctgat tatggactcc
     7141 ctcacccgct acgcgatggc ccagcgtgaa attgcgctgg cgattggcga accacccgcc
     7201 accaaaggtt atccaccgtc ggtgtttgcc aaattaccgg cactggtcga gcgtgccgga
     7261 aatggcatta gcggcggcgg ctcgattacc gcgttttata ccgtgcttac tgaaggcgat
     7321 gaccagcagg accccattgc agactccgca cgggcgatcc tcgacggtca cattgtgctg
     7381 tctcgccgac tggcggaagc cgggcactat ccggctatcg atattgaagc gtcgatcagc
     7441 cgcgcaatga cggcgttgat cagtgagcaa cattacgcgc gagtgcgcac cttcaaacag
     7501 ctgttgtcga gttttcagcg taaccgcgat ctggttagcg tcggcgcgta tgccaaaggc
     7561 agcgatccga tgctcgataa agccatcgcc ctgtggccgc agctggaggg ctatttacaa
     7621 caaggcattt ttgaacgtgc ggactgggaa gcgtctctcc aggggctgga gcgtattttc
     7681 ccgacagtgt cataacccag gagataacgg tagatggcag aacatggtgc gctggcgacc
     7741 ctgaaagatc tggcagaaaa agaggtagag gatgccgcgc gcctgctggg tgaaatgcgt
     7801 cgcgggtgtc agcaggcgga agaacagctc aaaatgctga tcgattatca gaatgaatat
     7861 cgcaataacc tcaacagcga tatgagtgcc gggatgacca gcaaccgctg gatcaactat
     7921 cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca acttaatcag
     7981 tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca acgtttgcag
     8041 gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga aaaccgcctc
     8101 gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc tgaatgattc
     8161 gcttagcgcc cttgattacc gccgacgttg acaccaccac attgcctggc ggcaaagcca
     8221 gcgatgctgc acaagatttt ctcgcgttgt tgagcgaagc attagcaagc gagacaacta
     8281 ccgacaaagc ggccccccag gtgctggtgg caacagataa gcccacgaca aaaggcgagc
     8341 tgctgatcag cgatattgtt tccgacgcgc aacaagcgga tttactgatc cctgtggatg
     8401 aaacactgcc tgtcatcaac gtcgaacaat ccacatcgac accgttaacc accgctcata
     8461 cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa gcggatgatc
     8521 tgaatgaaga cctcaccgca agcctgagcg ccctttttgc gatgttgccg ggttttgaca
     8581 atacgcccaa agtgactgat gcgccgtcaa ccgtgttacc ggcagagaaa ccaacgctct
     8641 tcacaaaact gacttctgcg caactcacaa cagcacagcc tgatgatgcc cccggcacgc
     8701 cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa gtcatcagca
     8761 caccttcacc ggtgaccgct gccgccagcc cgctaatcac tccacaccag acacagccac
     8821 tgctcaccgt cgccgcgcct gtcttgagtg caccgctggg ttctcacgaa tggcaacaat
     8881 cattaagcca gcatatttcg ctgttcaccc gccagggaca acaaagtgca gagttgcgtc
     8941 tgcacccgca ggatttaggt gaagtgcaaa tctccctcaa agtggatgat aaccaggcgc
     9001 aaatccagat ggtttcgccg catcagcacg tacgcgccgc cctggaagca gcgctgccgg
     9061 tactgcgtac gcagctggcc gagagtggca ttcagttagg gcaaagcaac atcagtggcg
     9121 aaagctttag tggtcagcag caggccgctt cccaacaaca gcaaagccaa cgcacagtaa
     9181 accatgaacc tctggcgggg gaagaagacg atacgcttcc ggttcccgtc tctttacaag
     9241 ggcgcgtaac aggcaacagc ggcgttgata ttttcgccta acgtcagagg tagcaccgta
     9301 atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc cgataagcag
     9361 tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga aaagcaagcg
     9421 atcgctttgg ctcccgattc tggtattcat taccctcgcg gcctgtgcca gcgcaggtta
     9481 cagctactgg cattcgcatc aggttgccgc tgaagacaaa gcgcagcatc gcgtcgtgcc
     9541 ctcaccggtc ttctatgcgc tggatacctt tacggtcaat ttgggcgatg cggatcgcgt
     9601 gctttatatc ggtataaccc tgcgactcaa agatgaagct acccgctcgc ggctgagtga
     9661 gtatttgccg gaagtccgta gccgtttgct gttactgttt tcgcgtcagg atgctgccgt
     9721 actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca cgctttccac
     9781 cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgatgtata ccgcttttat
     9841 tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat gcgctgttga
     9901 atggtgacag cgaagtcaaa gacgaaccga cagccagtgt tagcggcgaa agtgacattc
     9961 gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag gcgctggaaa
    10021 tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg ctgcgtcgta
    10081 gcccggatat aaccgtcggg gccatccgca ttcagccgta ccatgaattt gcccgcaacc
    10141 tgccggtgcc gaccaacctg aaccttatcc atctgaaacc gctgcgcggc acagggctgg
    10201 tggtgttttc accgagtctg gtgtttatcg ccgtggataa cctgtttggc ggcaatggac
    10261 gcttcccgac caaagtggaa ggtcgcgagt ttacccatac cgaacagcgc gtcatcaacc
    10321 gcatgttaaa gctggcgctt gaaggctata gcgacgcctg gaaggcgatt aatccgctgg
    10381 aagttgagta cgtgcgttcg gaaatgcagg tgaaatttac caatatcacc acctcgccga
    10441 acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc ggcgaattta
    10501 atatctgtct gccattcagc atgatcgagc cgctacggga attgttggtt aacccgccgc
    10561 tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc caggtgcagc
    10621 attcacagct ggagctggtc gccaactttg ccgatatctc gctacgcctg tcgcagattt
    10681 taaaactgaa acccggcgac gtcctgccga tagaaaaacc cgatggcatc atcgcccatg
    10741 ttgacggcgt cccggtgctg accagtcagt acggcactct caacggtcag tatgcgttac
    10801 ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag cccaaatgag
    10861 tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt gggctgaagc
    10921 gttgagcgaa caaaaatcaa ccagcagcaa aagcgctgcc gacgcggtgt tccagcaatt
    10981 tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgataatgg atattccggt
    11041 caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt tgcgtctgac
    11101 gcaagggtcc gtcgtggcgc tggacggtct ggcgggcgaa ccactggata ttctgatcaa
    11161 cggttattta atctcccagg gcgaagtggt ggtcgttgcc gataaatatg gcgtgcggat
    11221 caccgatatc attactccgt ctgagcgaat gcgtcgcctg agccgttagt gatgaataac
    11281 cacgctactg tgcaatcttc cacgccggtt tctgctgcgc cactgctgca ggtcagcggc
    11341 gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa acggttggga
    11401 tttgccccga aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc ttcgctgggc
    11461 gcgcgtgaac gggttgtggt ggtcgatgtg gaagatgcac ggctggtgct cggcgttacc
    11521 gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacaga agagataccg
    11581 cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc gtagtgggag atcctgatgc
    11641 gtcgtttgtt gtctgtcgca cctgtccttc tctggctggt tacgcccctc gccttcgcgc
    11701 aactgccggg tatcaccagc cagccgctgc ctggcggtgg acaaagctgg tcgctcccgg
    11761 tgcagacgct ggtgttcatc acctcgttga cgtttattcc ggcaatttta ctgatgatga
    11821 ccagtttcac ccgcatcatc attgtttttg gtttattgcg taacgcgctg ggaacgccct
    11881 ccgcgccacc taaccaggta ttgctggggc tggcactatt tttgaccttt tttattatgt
    11941 catcggtgat cgacaaaatt tatgtcgatg cgtaccagcc attcagcgaa gagaaaatat
    12001 caatgcagga ggcgctggaa aaaggggcgc agccgctacg tgagtttatg ctgcgtcaga
    12061 cccgtgaggc agatttaggg ttgtttgcca gactagcgaa taccggcccg ttgcagggac
    12121 ctgaagccgt gccgatgcgc attttgctcc cggcctacgt gaccagcgaa ttgaaaaccg
    12181 catttcagat aggcttcacg attttcattc cttttttgat tatcgacctg gtgattgcca
    12241 gcgtgttgat ggcattgggg atgatgatgg tcccccccgc caccattgct ctgcccttta
    12301 aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg ctggcgcaga
    12361 gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg gggactgaag
    12421 cgatgaaagt cgcgctggca ctggctgccc cgctgttgct ggtggcgctg gtcacgggcc
    12481 ttatcatcag tattttgcag gccgccacgc agatcaacga aatgacgctg tcgtttattc
    12541 caaaaatcat cgccgtattt attgccatta ttattgccgg accgtggatg ctcaatctgt
    12601 tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg taggccgtac
    12661 aatgatgcag gtgacaagcg atcaatggct ttcctggtta agcctgtact tctggccgtt
    12721 actgcgcgtg ctggcgctca tttctaccgc accaattctg agcgaacgca gcgtgccgaa
    12781 acgggtaaaa ctggggctgg caatgatgat cacgttcgtc attgccccat cattacctgc
    12841 caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtgcagc agatcctgat
    12901 cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa ccgctggcga
    12961 aattatcggt ctgcaaatgg ggctgtcatt tgcgacgttt gtcgatccgg ccagccatct
    13021 caatatgccc gttttagcgc gtatcatgga tatgctggcg ttactgctgt tcctgacatt
    13081 taacggtcat ttatggttga tttcactgct ggtcgatacc tttcacaccc tgccaattgg
    13141 tggcgaaccg ttgaacagca atgcgtttct ggcactcacc aaagcgggga gtttgatttt
    13201 ccttaacggg ctgatgctgg cgttaccgct cattactctg ctgctgaccc tgaatctggc
    13261 attaggttta ctgaatcgta tggccccgca attatccatt tttgttattg gatttccatt
    13321 aactctgact gtcggtatct ctttaatggc ggcattaatg ccgttaattg cacctttttg
    13381 cgaacattta ttcagtgaga tgtttaattt gctggctgat attattagtg aattgccatt
    13441 aatataattc cgtaacgttt atcatgttat cctaaggatt atccgaaaaa taatacctac
    13501 gaacatcttc caggatactc ctgcggcgaa atatttgttt taagctcact cacatatcgc
    13561 aacatttact ttaagacaat tccaggcaaa ttatacaaca ctttacggga tagtaagtcc
    13621 gcctgaaaaa tcgcgagagt ggcgcattag gtgacccatg ttgttccgtt tagtcatgat
    13681 gaaatattca ggtaagggga attatcgtta cgcattgagt gagggtatgc catgtcaacg
    13741 attattatgg atttatgtag ttacacccga ctaggtttaa ccgggtatct gttgagtaga
    13801 ggggttaaaa aaagagaaat caacgacatt gaaaccgttg atgaccttgc catagcttgt
    13861 gattcacagc gcccttcagt ggtgtttatt aatgaggact gtttcatcca tgatgcttct
    13921 aacagtcagc acatcaagca catcattaat caacatccca atacgttatt tatcgttttt
    13981 atggcaattg ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa attattgata
    14041 agttctaaat cgattaaacc ggaatctctc gacgatatcc ttggcgatat tctgaaaaaa
    14101 gagacaacga taacctcttt tttaaatatg ccgacgttat cattgagccg aaccgaatcg
    14161 agtatgttgc gaatgtggat ggcaggtcag ggaaccattc aaatctctga ccaaatgaat
    14221 atcaaagcca agaccgtttc atcgcataaa ggtaatatta aacgtaagat caaaacacat
    14281 aataaacagg ttatctacca tgtcgtccga ctgacggata atgtgactaa tggtattttt
    14341 gtcaacatgc gctaacacat tctgactggt ggtttcccac cagtcaggct gaataagatt
    14401 actctgcttt ctccacaaag ataccgtcct gatgccctgc ttcattaaag aaccagatgc
    14461 cgagcgggta gtcttccagc gaaaccaggt acattgtgcc ttcactaaac tcctcaactg
    14521 ccagtaccac gccaggacga cgcggaccgc catccgtttt gactgttacc cgatcattca
    14581 ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga acaatttcgt ttttctggca
    14641 gctccaggcg cgcgcgagtg ctgattttct cgacggtcta tacttaagag atgccaggcg
    14701 gacttaacga ctggcggcaa caacagagta acggttgcga ggaaagatga tgaaaaccgc
    14761 aaaagagtac agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg ccctgctggc
    14821 ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt ctgaacacgt
    14881 tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct tcgaactgga
    14941 tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa aaatcccgac
    15001 cctgaggggg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat tcgttacacc
    15061 aggaaatctg atgtgttcat caccttatcc gcaatttttt tcgctgacaa gaaaatattc
    15121 gcgatatgaa taattaacgc tacttatgaa tagaaatgtg acgtgcgtca ccccttggtg
    15181 gctctggcgt caacgggatc gctcccgaaa aaatcatctt tgattatttg tgctgttttg
    15241 agaatcatcc tggtaagggg agaactgcac gaccatgttt tcaattcaac aaccactact
    15301 ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact ggcaaccgac
    15361 tgccctctgg ctcagccgtt tacgcgaagc gaatgttccc gtcattctct gtagcagtaa
    15421 aacttcagcg gaaatgctgt acttgcaaaa aacgttgggg ctacaagggt taccactgat
    15481 tgccgaaaat ggcgcagtga tccagcttgc tgaacaatgg caggatatag acggttttcc
    15541 acgcatcatc tcaggtatta gccatggcga aatcagccag gttttaaata cgctacgcga
    15601 gaaagaacat tttaaattca cgactttcga tgatgtcgac gatgcaacca tcgccgaatg
    15661 gacgggatta agccgtagcc aggcggcgct gacgcagctg catgaggcgt cggtaacgct
    15721 aatctggcgc gacagtgacg agcgtatggc acaatttacc gctcgtctga acgaactggg
    15781 cttacagttt atgcagggtg cgcgcttctg gcacgtcctg gatgcctctg ccggaaaaga
    15841 tcaggccgcc aactggatta tcgcgaccta tcaacaatta tcaggcaaac gcccaaccac
    15901 acttggcctg ggcgatgggc caaacgatgc gcccttactg gaggtaatgg attacgcggt
    15961 gattgtgaaa gggctaaacc gtgaaggggt gcatctgcat gatgaggatc cgacccgcgt
    16021 ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa gggctggacc attttttctc
    16081 cgctcgttaa gcgttatcgc tcgcgcatac ccgattacgc ccggcctgtt tagcgagata
    16141 aagccgacgg tcagccagtg actggagttg ttcaaaatca taatcaccgg tttcctcgct
    16201 gctacttacc cccaacgagg cactgatgcg tatcgtcgta ctcttagcaa tcaacatctc
    16261 tttttcattt aacttcaggc gaatacgttc tgcgacttcc gcagcctccg tcagactcgc
    16321 gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt cctgcgcacg
    16381 caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg cctgatgacc
    16441 aaagcggtca ttaatcgctt taaaatggtc aaggtcgacc tgaatgacag aaaaaggatg
    16501 ttggtgcgtc tgacacaatt tcgcgagcgg acgggctttt tcaaacagtg cgccacgatt
    16561 atataaacgg gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt gcagaacata
    16621 catgttgcta accatccggc gaatcacata ccaggagatg agtaacatgg tggtaaagag
    16681 cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca cgccttcgct
    16741 tagcgtatgg acgcgcacca gcacaccgtc gaaatggtcc agacgttccc agctaacata
    16801 gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc atcgcctgcg ccagcaaggc
    16861 cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag aggtcaaaaa
    16921 tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga tggcgtttct
    16981 taaaaattgc tgcatggtac gcacgggaat actcatcccc agcacgccat accagtagtt
    17041 attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg cgtgttccgg
    17101 ttgcgaagta aaccagcgta ccgcacggtg acgattttct cgttgcgaat ggccgataaa
    17161 ccagggttgg gtgacatagc cgtaataacg cgttggtaca ttgcgcgtaa acaaggtcgg
    17221 ctgcgtcgaa acgtaaaatc cggcacgcga gacatacatc gcctgttcaa ccatcgacga
    17281 ggtgttgtgc gccagtcgca gcaagtaacc aacttccagt gcagcggtaa tttcattgtc
    17341 gaggctttca ttttcgcgag acaggagatt cccctcgctg actaatgcat ccgatacgcc
    17401 attgactgac agggtgcgtc gtcggttgag ttcaatttgc caggcgtgct cgtcgcgatg
    17461 ctgttcgaac tcggttacag cattacgcag tgaagtgaaa tccagtggcg ctacgagagc
    17521 ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca aatgcttatc
    17581 gagcgcgttg gctacgtttt ccagatgatt acgctggctg gagatatagg catcttccag
    17641 cactaccact tcacgccagg tcagcaaggt ggaaaaaagc aataccacga taaagcagag
    17701 attaacgaca tgaccaggcc ccaggcggcg tgcgagtttt ttcaaccagc tctggttttc
    17761 catttttgtc tcgtgctgca ccctgacgac tcccaacctg cttttttatg attctggcat
    17821 aacctggcga tagcgtaccg caaaacagcg ataattttat aatggtggca tgataagacg
    17881 cgactatcat tcattgcagg cattggcaca ccaggccgga agtgatccgg cctggtgtta
    17941 atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac aagctcggtt
    18001 tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc aaccataccg
    18061 ctggagtatc cagtgacaat catccgcggg ccgccctctt taaccgtaac ttcctcacta
    18121 accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct tagttgaaga
    18181 tggcgaattt tgcataatag cctgtacgag tattaatgtg cctgaccgcg cattttcgct
    18241 acggctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat cagatttaaa
    18301 acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgaaatg ttcaatggcg
    18361 tgatgcagcg gcgcaatacc atgtaccaca ataccgccgc cgacgaggaa catcgccagc
    18421 gtgccgacaa tcgataacgc cttcatcaac cagggcgcaa tgatcaataa ccctttacct
    18481 aatgcctgca tcagcgcgct ggatttttcc gccagccaat accccaggtc atcaatctta
    18541 acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat gcctgaaagc
    18601 accagcacct gattaagcaa cggcgcttcg gccacaatcc ccagcgtgat ggcgacgatt
    18661 tcggcagaca atataaaatc ggtacgaatc gcccctttta ttttgtcctt ttcaaacttc
    18721 agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgccgg atcttcttta
    18781 tgtttacgcg cctccagcat atgcagcact ttctctactc cttcaaagca gagaaacgcg
    18841 ccaccaatca tcaacagagg cgtaatcgcc cacgggataa acgcactgat gatcagcgcc
    18901 agcggcacca gaatcacttt attaatcagc gatcctttcg ccacgcccca gaccacggga
    18961 agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa gtcatcccct
    19021 aatacaccgg cggttttctt cgccgccagt ttgcccatca cggagatatc gtccagtagt
    19081 gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat
    19141 ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac ttgcttcaca
    19201 ggtcaacatt ttttttacaa tcaagtgcaa ttatatctct cgccagcaga atgattttca
    19261 cgtttactat gaaccacctt tttatttcca ccgtgaggga ttatgcgttt ccgccagttg
    19321 ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac ctattttgtc
    19381 attcggattg gcgtggaaag ctggcctccg ttaatgatgg caggcgttcg attcctggca
    19441 gccggtattt tattgatggc atttttgcta ctgcgcggac acaagctccc cccgctacgt
    19501 ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa tggcatggtg
    19561 acggttgccg agcatcaaaa tgttccttcc ggcatcgccg ccgttgtggt tgcaaccgtg
    19621 cccctcttta ccctggtgct tcagccgcct gtttggcatt aaaacgcgca aactggaatg
    19681 ggtgggtatc gccattgggc ttgccggaat catcatgctc aatagcggtg gaaatttaag
    19741 cggcaatccg tggggcgcga ttctgatttt aatcggctcg attagctggg cgtttggctc
    19801 ggtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga ttgagatgct
    19861 ggcggcaggc gtggtgttaa tgatcgcgtc gatgattgca ggtgaaaaac tgacggcgct
    19921 cccttccctt tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt cgattatcgc
    19981 catcaacgct tatatgtatt taatccgtaa tgtcagtccg gctctcgcca ccagctacgc
    20041 ctacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggcggag agacgctgtc
    20101 gaagattgaa tggctggcgc tcggcgtaat tgtcttcgcg gtggtactgg tcacgttggg
    20161 aaaatatctc ttcccggcaa aacccgtagt tgcgccagtt attcaggacg catcaagcga
    20221 gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag atccactctt
    20281 ccagacgctc ggtaagcgct tcatccgtca gcttctcgcg cccacgtaat gcacactccc
    20341 agacaatcag tacgcgccag ccgagttcct gcaaacgact gatatcgcgg cgatcgcgtt
    20401 caacattttt acctatcttc tccagccaga actcggttcg agtcgcaggc actttaaaca
    20461 gatagcagtg atgatgatgc cagaagcagc catgggtaaa tatcacgcag cgatattcat
    20521 caacgacaaa atccgggctt ccgggcagac tggcgtcctg aacgcgaaac gccaggccct
    20581 gcccggttaa cagactggcg aggcgcttct caatcgccgt atcacgcgtg gcaatcgcgc
    20641 gcatattttt gctgcgagtg gccttatcgt gaacgtcggc catgttgtgc ctcttgctga
    20701 cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc ggcaaagacc
    20761 ggcacgacca ccgagttacc gaactggcga taggcctgag tgtccgaaac cggaatgcgg
    20821 aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcccg aggcgttaac
    20881 cgacgtggac gatgttgctg attcagcgga tcgtcaaaat ctttctcacc cgtggccata
    20941 tcccagccgc gatcgattaa aatttccgcg ccatctttgt aataacgcgc agacagcgta
    21001 cgcgtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc gttaccgcgc
    21061 gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt caggatatat
    21121 ttcgcctcga ccatcgggtc caacagctgc gccagcgtca ctcgctgtgc agggaaacat
    21181 tcgctgatat cacgcagagt aaaatcggct ttcagattaa gatcgcgacg aaaacccacc
    21241 agcacgatgc gttcacggtg ctgcggcaga aaatgtttac catcgatgat tttcggatcg
    21301 tccggcccgt tatcttctgc atcagccacg tcatagccca gttcgtccag cgtctgcatg
    21361 atgatgcgga acgttttacc ctggtcgtga cttttcaggt ttttgacgtt ttcgagcaca
    21421 aacatcgccg gacgacgcgc gtcgataatg cgtaccacat caaaaaacag cgtgccctgg
    21481 gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac gccagccagc
    21541 gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat gtgttgacga
    21601 atatgttccg ccgccgcctc atcactcacg ccttctttat ggctgagggt gatgtcgcgg
    21661 atatcttcat taaaatgatg cgtcgccgga tcgcaataat ggttggcttt ataagtgcgt
    21721 accgcatgtt tgttccattc gctggtaaac acgcattgtc cgccaatcga ttcaaaaccg
    21781 cgacggatgc cgccaattcc ggcaaataga tcgataaagc gaaacgcata atgcggatga
    21841 tgtgccgatg gtttgggtaa caacgtttgc agatgggcga actctttctc acttaaacgg
    21901 tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca gtgattctca
    21961 cccacaccat taagctgcgc caccagcgtt ttaacatcat aaatttgcag cagtttctcc
    22021 agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat attttcctgc
    22081 atagatttaa ccggccatct gagatggccg acaggttaac ataattacag ccatttaggg
    22141 ccacgcgctt atcccgcggg tgaaaacgca tctatcacct tgtgatcgac accttcattc
    22201 tcccccgcca gttcggcact gagcttcgcc ataaactcga ctaaaaaatg cgcgttgtgc
    22261 tgcgccagct gcttgcctct tgcggtttgc atggtttgcg gtagcttcag caacttagtc
    22321 tggaaatgat ccagcgcgta gcgtttatca tcaagcgggc gatgctgtgc gaacggatct
    22381 tcaccatcaa acagcgccac gcccaatgcc cctgaaacgg caaacacgcg cgccaggcca
    22441 atcgccccca gggcttccag ccgatccgca tcctggacga ttttagcttc cgttgttaag
    22501 ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc ctcgattttc
    22561 tccgccggaa attgctcgaa ctcttcacgg agcaggcgac gcgtctcttc tgccgccagg
    22621 attgaagaac gctgcctttg cggatgattt ttcgccaggc tcacaatatc gtgaaaataa
    22681 caggcggtta aaatcaccag catatcgaca tcgtcatctg ccgccagctt ctgtgccgcg
    22741 tcctggtgct gatgatggtt tttcaaccag ttttcgaact gtgcctgcca gtgttgtaag
    22801 tccatacgcg ctccgttgcc aaagagtcgc caccttagca gtttttattc atagcgtctg
    22861 ctacgtgcgg gacgaggtcg ggtgtaccag gcaatgctgc ctaacaatgc cccgaccaga
    22921 gccataaata aaaatccgac cagtgcccct aaccactttc ccgcggtaga gcctaaatcg
    22981 ctttcaaatc ccgacaccgg ggcatcaggg atccggctca tcacccagat gtaacgcgcc
    23041 attgcccaga cgaaatagcc acaccatgta tagaaagccc acaaagccaa tttgccgcca
    23101 gggctgcgag agagtctctc ttccatcttt gtcgaaccgt attccatttt gagataaccc
    23161 ggaaaattca gacttagtac tattatgtga taaaagtcac atttttccac attggatgag
    23221 ccagtcaaga ctcaatcatt ttttgaacaa atagataaat tttgcgcaac ggcaataatt
    23281 actggaaaaa cagcaataaa gatttattta tatc
//
LOCUS       DAZSBF010000050        23235 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZSBF010000050 DAZSBF010000000
VERSION     DAZSBF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.051"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="ACHXWD_001521"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="ACHXWD_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR7620466.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="ACHXWD_001522"
     CDS             507..1136
                     /locus_tag="ACHXWD_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR7620467.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="ACHXWD_001523"
     CDS             1163..2824
                     /locus_tag="ACHXWD_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR7620468.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="ACHXWD_001524"
     CDS             3063..3122
                     /locus_tag="ACHXWD_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7620469.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="ACHXWD_001525"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="ACHXWD_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR7620470.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="ACHXWD_001526"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="ACHXWD_001526"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR7620471.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="ACHXWD_001527"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="ACHXWD_001527"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR7620472.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="ACHXWD_001528"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="ACHXWD_001528"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR7620473.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="ACHXWD_001529"
     CDS             9505..10125
                     /locus_tag="ACHXWD_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR7620474.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="ACHXWD_001530"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="ACHXWD_001530"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR7620475.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="ACHXWD_001531"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="ACHXWD_001531"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR7620476.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="ACHXWD_001532"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="ACHXWD_001532"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR7620477.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="ACHXWD_001533"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="ACHXWD_001533"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR7620478.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWSVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="ACHXWD_001534"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="ACHXWD_001534"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="ACHXWD_001535"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="ACHXWD_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR7620479.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="ACHXWD_001536"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="ACHXWD_001536"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR7620480.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="ACHXWD_001537"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="ACHXWD_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR7620481.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="ACHXWD_001538"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="ACHXWD_001538"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR7620482.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="ACHXWD_001539"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="ACHXWD_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR7620483.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="ACHXWD_001540"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="ACHXWD_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR7620484.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="ACHXWD_001541"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="ACHXWD_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR7620485.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5652 a         6330 c         5907 g         5346 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc gtgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt gcacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggtccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DAZSBF010000051        22720 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZSBF010000051 DAZSBF010000000
VERSION     DAZSBF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.052"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..440)
                     /gene="folK"
                     /locus_tag="ACHXWD_001542"
     CDS             complement(<1..440)
                     /gene="folK"
                     /locus_tag="ACHXWD_001542"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR7620486.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREV"
     gene            complement(437..1834)
                     /gene="pcnB"
                     /locus_tag="ACHXWD_001543"
     CDS             complement(437..1834)
                     /gene="pcnB"
                     /locus_tag="ACHXWD_001543"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR7620487.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1894..2790)
                     /gene="gluQRS"
                     /locus_tag="ACHXWD_001544"
     CDS             complement(1894..2790)
                     /gene="gluQRS"
                     /locus_tag="ACHXWD_001544"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR7620488.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2857..3312)
                     /gene="dksA"
                     /locus_tag="ACHXWD_001545"
     CDS             complement(2857..3312)
                     /gene="dksA"
                     /locus_tag="ACHXWD_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR7620489.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3490..4194)
                     /gene="sfsA"
                     /locus_tag="ACHXWD_001546"
     CDS             complement(3490..4194)
                     /gene="sfsA"
                     /locus_tag="ACHXWD_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR7620490.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4209..4739)
                     /gene="thpR"
                     /locus_tag="ACHXWD_001547"
     CDS             complement(4209..4739)
                     /gene="thpR"
                     /locus_tag="ACHXWD_001547"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR7620491.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4813..7242
                     /gene="hrpB"
                     /locus_tag="ACHXWD_001548"
     CDS             4813..7242
                     /gene="hrpB"
                     /locus_tag="ACHXWD_001548"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR7620492.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7438..9972
                     /gene="mrcB"
                     /locus_tag="ACHXWD_001549"
     CDS             7438..9972
                     /gene="mrcB"
                     /locus_tag="ACHXWD_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR7620493.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10192..12381
                     /gene="fhuA"
                     /locus_tag="ACHXWD_001550"
     CDS             10192..12381
                     /gene="fhuA"
                     /locus_tag="ACHXWD_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR7620494.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12432..13229
                     /gene="fhuC"
                     /locus_tag="ACHXWD_001551"
     CDS             12432..13229
                     /gene="fhuC"
                     /locus_tag="ACHXWD_001551"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR7620495.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13229..14119
                     /gene="fhuD"
                     /locus_tag="ACHXWD_001552"
     CDS             13229..14119
                     /gene="fhuD"
                     /locus_tag="ACHXWD_001552"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR7620496.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14116..16098
                     /gene="fhuB"
                     /locus_tag="ACHXWD_001553"
     CDS             14116..16098
                     /gene="fhuB"
                     /locus_tag="ACHXWD_001553"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR7620497.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16256..17536)
                     /gene="hemL"
                     /locus_tag="ACHXWD_001554"
     CDS             complement(16256..17536)
                     /gene="hemL"
                     /locus_tag="ACHXWD_001554"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR7620498.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17702..17749
                     /gene="yadX"
                     /locus_tag="ACHXWD_001555"
     CDS             17702..17749
                     /gene="yadX"
                     /locus_tag="ACHXWD_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR7620499.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17761..19182
                     /gene="clcA"
                     /locus_tag="ACHXWD_001556"
     CDS             17761..19182
                     /gene="clcA"
                     /locus_tag="ACHXWD_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR7620500.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19206..19271
                     /gene="yadW"
                     /locus_tag="ACHXWD_001557"
     CDS             19206..19271
                     /gene="yadW"
                     /locus_tag="ACHXWD_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR7620501.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19264..19608
                     /gene="erpA"
                     /locus_tag="ACHXWD_001558"
     CDS             19264..19608
                     /gene="erpA"
                     /locus_tag="ACHXWD_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR7620502.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19655..20278)
                     /locus_tag="ACHXWD_001559"
     CDS             complement(19655..20278)
                     /locus_tag="ACHXWD_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR7620503.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20316..21116)
                     /gene="btuF"
                     /locus_tag="ACHXWD_001560"
     CDS             complement(20316..21116)
                     /gene="btuF"
                     /locus_tag="ACHXWD_001560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR7620504.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21109..21807)
                     /gene="mtnN"
                     /locus_tag="ACHXWD_001561"
     CDS             complement(21109..21807)
                     /gene="mtnN"
                     /locus_tag="ACHXWD_001561"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR7620505.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21891..>22720
                     /gene="dgt"
                     /locus_tag="ACHXWD_001562"
     CDS             21891..>22720
                     /gene="dgt"
                     /locus_tag="ACHXWD_001562"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR7620506.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         5139 a         5794 c         6354 g         5433 t
ORIGIN      
        1 acttcacgca acgtctcccc atcaggaaac gccagctccg gtgcaatctc aaacagcggc
       61 cacagcataa atccacgatt cttcatatcg tagtgcggaa cggtcaggcg ttcggtattt
      121 atcacttcat taccaaacag catgatatcg agatccagcg tgcgtggtcc ccagcgttca
      181 gctttacgaa cacgaccctg ttgcagttct atgcgctgtg tatggttgag taactcttcc
      241 ggggcgagcg aagtttcaag cgccacggcg gcgttgaggt aatcgggttg atcttgcggc
      301 cccagcggtg gagtgcggta aaacgaagaa acggcaagaa tgtggctttc agggatatcg
      361 cctaatgctt tcagggcagc attgacctgc tccagcggag aggccagatt gctgcctatg
      421 gcaatatacg ccactgtcat gcggtaccct cacgacgcgg tgcgcgtttg cgtggacgac
      481 gagtacggcg acgcggtgac ggctcttcat ccaactcgtt aagcatccct ttttgatctg
      541 gtggcgcgga aacctggaac tcaccccacc atttcaccag acgctgcagt tcagcgttac
      601 gttcaacttc agctcgcaag gccaacaggt cataagccgc acggaactta ggatgctcca
      661 gcagtttcca tgcgcgttta ccctgacgtc gggacatacg caactgcaac tgccagatat
      721 cgcgggttaa cgtcgtcagg cgtttcggga ttgccagtga acggcaggct tcgtccagca
      781 cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag gccgctttcc tgggcgatct
      841 tctgtgccgt ctccagcagt gggtaccaga acatggcggc aaacaagaac gccgggttca
      901 cgcgcatatc gttatggata cgcgtatcgg tattcttcag cacctgttca atgatccgct
      961 ccatcgggct gtcgccattt tccgtgaagt agcgggtaat ggtcgggaac agcggctgga
     1021 acagatgata ttcacacaac agcttatagg tttcgtaacc gtagcccgct tgtagcagtt
     1081 taagcgattc ttcaaacagg cgtgccggtg ggatatcgtt cagcagggta gcgaggcgag
     1141 ggatcggttc tgcggtttcc gggctgatgc gcatacccaa tttggcggca aaacgtaccg
     1201 cgcgcagcat ccgtaccgga tcttcacggt agcgcgtttc cgggttacca atcagacgga
     1261 taacgccgtc cttcagatcc ttcataccgc caacgtaatc acggacggta aaatccgcta
     1321 cgctgtaata gaggctgttg atagtgaaat cgcggcgctg ggcgtcttct tcgatggagc
     1381 cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg ttgggaggtc gtgcggtcgc
     1441 tgacgttacc ttcgtggtgt ccacggaagg tcgcaacttc gataatctcc gggccaaaca
     1501 ttacatgagc cagacggaaa cggcgaccca ccaggcggca gttacggaac agtttgcgca
     1561 cctgctcagg cgtggcgtta gtggttacgt caaaatcttt cggctttttg ccaagtaaca
     1621 ggtcgcgcac gccgccgcca accagccagg cttcgtatcc cgctttgttg agcctgtaca
     1681 ttaccttcag ggcattttca ctgatatctt tgcgggaaat agcatgctgc tcacgcggga
     1741 tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc gctttcctcg cggcttagca
     1801 ccttgcggca aaaattagcg actcgggtaa aaatagtaca cctcggtagt gtcaaacatc
     1861 attcaggaca aaaaaatagc ggctaatcat agctcagcat gacgcatttg agaatgttga
     1921 atttacaatt gccgactcgg gcacggcggt cagcgtccag tttttgactg ctgactgaag
     1981 gatttgttcg acgctgaaat cctgccagtg tgcttctgcc tgctgaccca gaaattgaag
     2041 tgccgcgatt agtaccggac gtggatcgcc tttcggcaac gcaggcgcat gattctgctt
     2101 ggaaagttta gcgccttgtg gattaagcgc cagcggcaga tgaatgtaat ctggcacttt
     2161 ccagccaaaa agctggtaca gcgagatttg ccttactgtt ggttcaatca gatcagcccc
     2221 acgcactatt tctgtaacgc cctggaaatg atcatcaacc acaacagcca ggttgtaggc
     2281 gaacaaccca tcacggcgat gaatgataaa atcttcccgt gccagttttt cgtcggcgtg
     2341 aataatgccg cgcagctggt cagtaaattg cgtgaccgga tgctgctggc ggatacgcac
     2401 tgcggcattg tctggtccat gatgcaacac ccggcaatga ccgtcgtaaa taccgccaat
     2461 gctttgaata cgcgcacgcg tacaggtgca gtaataactt agtccttgtt catgtaacca
     2521 ggcgagtgct tcacgatagg cgtcgtgacg ttgcgattgc cagagaacat cgccgtccca
     2581 gtgcagaccg taatgttcca gctggcgcag gatagtttct gcggcaccgg gaacttcacg
     2641 aggcgggtcg atatcttcta tgcgtaccag ccagcgacct tgccgggcgc gagcctgcaa
     2701 atagctgccg agcgcggcga tcagagagcc aaaatgaagc tcgccggaag gagagggggc
     2761 gaagcggcca atatactgtg tgtctgtcat ctctttgaac aaaaaataag gcgggagcat
     2821 ttcccgcctg tggtaaacgt gatggaacgg ctgtaattag ccagccatct gtttttcgcg
     2881 aatttcagcc agcgttttgc agtcgatgca cagatcggct gtcgggcgcg cttccagacg
     2941 gcgaatacca atttcaacac cgcaggattc gcagtagccg aaatcttcgt cttccacttt
     3001 tttcagcgtc ttctcgatct ttttgatcag cttacgctcg cgatcgcggt tacgcagttc
     3061 gaggctgaac tcttcttcct gggctgcacg gtctaccggg tccgggaagt tggctgcttc
     3121 atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg agttgattac gccatgcttc
     3181 cagaatacga cggaagtgcg ccagctgggc ttcattcata tactcttcgc ccggcttctc
     3241 ctgatatggt tccaccccag cgatggcgag aatactcagg gacgatgttt tacggttttg
     3301 cccttcttgc atgttgcttc tccttaacac gcactatcga tccccatgtt cgggggaaaa
     3361 atgaggccgc tataaatagc agatgctttt ccggatagca attatctaaa cgtaacactt
     3421 gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca gaatgtaaat taaccagtta
     3481 cttactttac tacaatgtaa ccgacagtga ttttttaaga gccatgcctt cagcagaaat
     3541 ttccgctttg taagccagaa tttctacccc cctctgttga gcttctgaca atagttgcgc
     3601 gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta atggctgaat gcagcacggc
     3661 gaaaaagata accgcacgct ggccttcagc cgctacgttc atcaactccc gaaggtgttt
     3721 ctgacctcgt tcagtgaccg catcgggaaa atatccctgt tcgttctccg ctaacgtaac
     3781 cgatttcact tcaatatagc agtctggacg cgaatccgcc tgcaacataa agtcaatacg
     3841 gctgcgttct gcgccgtatt ttacttcgct tttcagcgag ctatagcctg acagttctga
     3901 aattgattca ttaaggatag cctctttcgt caacctgtta gcccaaagcg tattgacgca
     3961 aataaatgcg ccgctctggc tttgagttag ttcccaggtg tgtgggtatt tccgtttggt
     4021 gttgtctgaa gtcgaatacc agacggtatc gccacgcgtt gcacaaccgg tcatcgcacc
     4081 cgtattcggg cagtgtagcg ttaattcgcg accatcgggt gtgatcacat cggctaaaaa
     4141 acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg ggagagaatt ccattgcgac
     4201 aatccttgtt attgcgttag cgcccagcgt tttagcggcg tgtagcgtgt gcgtccacgg
     4261 gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgcat aagaccagtt aaaacctggc
     4321 ggcgggattg tcaccgcctc gctggcgtcg cgcaataagg taatatgtgg atgaaacgga
     4381 cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac ggagcatatt cgccagctgg
     4441 attaagccgc gtggcggctg acgcatccct aaccacacca cacgcgaacg cagccattgt
     4501 ccggcgtcat caagcgtgag tgtgaaacca ggttgacgaa tccgtccggc taaaagagaa
     4561 agcgccttct ctttctcggc gctcacttcg cctaaaaatg ccagagtcag atgcaaatta
     4621 tcggcggcga ccggacgtcc cgcctcaggt gggaagtgtg tggcgcgcca gtggataatc
     4681 tgttcgcgga tttctgcagg taagtcgata gcaaagaaca gacgttgcgg ttcagacatg
     4741 tgaggcactc ggttatgaat taccgcgatg ctacaatgtg gcgcgaagaa tgttaaccct
     4801 ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct tacctgaatt acttaccgct
     4861 ctcgattgtg cgccgcaggt attattaagt gcgccgaccg gggccgggaa atcaacctgg
     4921 ctgccgctgc aactgctggc gcatcccggc attaacggga aaattatcct gctggagccg
     4981 cgtcgtctgg cggcacgtaa cgtcgcgcaa cggctggcgg agctgcttaa cgaaaagcca
     5041 ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg tcgggccaaa tacccgtctg
     5101 gaagtagtta ccgaaggcgt gctgacgcgg atgatccagc gtgacccgga gttgagcggt
     5161 gttggattgg tgatccttga tgaatttcat gagcgcagct tgcaggcgga tttggcgttg
     5221 gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc ttaaactgct gattatgtcg
     5281 gctacgctgg ataacgaccg cttgcagcaa atgctgccag aagcgcccgt cgtcatctca
     5341 gaagggcgct cgtttccggt tgaacgccat tatttaccgc tgcccgcgca tcagcgtttt
     5401 gacgaagccg ttgcggtagc caccgccgaa atgctgcgtc aggaaagcgg atcattactg
     5461 ttatttttac ctggcgtcgg agaaattcag cgtgtgcagg aacaactggc ttcgcgcatc
     5521 ggcagtgatg tgttgctctg cccgctgtat ggcgcgttgt cgctgaacga tcagcgaaaa
     5581 gcgatcctcc cggcaccgca agggatgcgc aaagtggtgc tggcgaccaa tattgctgaa
     5641 accagtttaa ccattgaagg tattcgtctg gtggtggatt gtgcccagga gcgtgtggcg
     5701 cgttttgagc cgcgcacggg gcttacgcga ctgattactc aacgcgttag ccaggcatcc
     5761 atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg gtatctgcct gcatttaatc
     5821 gccaaagaac aagcagaacg cgctgccgcg caaagtgaac cggagatctt acaaagcgat
     5881 ctttccggtt tgctgatgga attactgcaa tggggatgca gcgatccggc gcagatgagc
     5941 tggctggatc aaccgccagt agtgaatcta atggccgcga aacgtctgtt acaaatgctg
     6001 ggggcgctgg acggtgaacg gcttagtgcg caagggcaaa aaatggcagc gctgggtaac
     6061 gatccgcgtt tagcggcaat gctggttagc gcgaagaacg acgacgaagc tgctaccgcg
     6121 gcaaaaattg ccgccattct cgaagagccg ccactgatgg gcaatagtga cctgggcgtg
     6181 gcgttttcgc gcaatcaacc agcctggcag caacgtagtc agcaactgtt aaaacgctta
     6241 aacgtacgcg gcggtgaggc agacagttcg cttatcgcgc cgctacttgc cggggcgttt
     6301 gccgatcgca ttgctcatcg ccgtgggcaa gatggacgct atcaactggc aaacggcatg
     6361 ggagcgatgc tcgatgccga cgacgcgcta agccgccacg aatggttgat cgcaccgtta
     6421 ttattgcagg gcagcgcctc gccggatgcg cggattttac tggcgctacc ggtcgatatt
     6481 gatgaattag tacaacgctg tccgcagctg gtgcagcaat ctgacaccgt ggaatgggat
     6541 gacgcgcaag gtacgctgaa agcctggcgt cggctacaaa tcggtcagtt gacggtgaaa
     6601 gtgcagccgc tggcgaaacc gtcagaagac gagttgcatc aggcgatgct taatggcatc
     6661 cgtgataaag gtttaagcgt gctcaactgg acggcggaag cggaacagct acgcttgcgt
     6721 ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc cagcggttga tgatgaaagt
     6781 ttattggcag cgctggaaac gtggctgctg ccacatatga caggcgtaca ttcactacgc
     6841 ggcctgaaat cactggacat ttatcaggca ctacgcggat tacttgattg gggaatgcag
     6901 caacgtctgg atagtgaatt gcctgcgcat tacactgtgc cgacgggaag ccggatcgcc
     6961 attcgttatc atgaagataa cccgcccgca ctggcggtga gaatgcagga gatgtttggt
     7021 gaggccacta atccgactat cgcccagggg ggcgtgccgc tggtgctgga gttgctttca
     7081 cccgcccaaa ggccactaca aatcacacga gatttgagcg ccttctggaa aggagcgtac
     7141 cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac acgtctggcc ggacgacccg
     7201 gcaaacacgg caccgacgcg acggacgaaa aagtattcgt aaggttggtt ttctccctct
     7261 ccctgtggga gagggccggg gtgagggcat cagcgcgcac gttcaccctc accctaaccc
     7321 tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc ttcttctgtc
     7381 ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag aaaaagcatg
     7441 gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc ggtcaaacaa
     7501 aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga tgactatgag
     7561 gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa gcctcgtggc
     7621 aaacgcggct ggctatggct gctgttaaaa ctggctatcg tttttgccgt gctgatcgcc
     7681 atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa ggtctggcaa
     7741 ctgcccgcgg cagtttatgg ccgaatggtc aaccttgaac cagatatgac catcagcaag
     7801 aacgaaatgg tgaagctgct ggaggcaaca cagtatcgtc aggtgtcgaa aatgacccgt
     7861 cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc gtttgatttc
     7921 ccggacagca aagaagggca ggtgcgtgcg cgtctgacct ttgatggcga tcatctggcg
     7981 acgatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga tccgcgcctg
     8041 atcaccatga tctcttcgcc aaacggtgag cagcgtctgt ttgtgccgcg cagtggtttc
     8101 ccggacttgc tggtggatac tttgctggcg acagaagacc gtcatttcta cgagcatgat
     8161 gggatcagtc tctactccat cggacgtgcg gtgctggcaa acctgaccgc cggacgcacg
     8221 gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa acctgttcct ctccagcgag
     8281 cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga cgcgcgttac
     8341 agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca gagcggcgac
     8401 aacgaaatcc gcggcttccc gcttgcgagc ttgtattact ttggtcgccc ggtagaagag
     8461 ctcagcctcg accagcaggc gctgttagtc ggtatggtga aaggggcgtc catctacaac
     8521 ccgtggcgta acccgaaact ggcgctggag cgacgtaatc tggtgctgcg tttgctacaa
     8581 caacagcaga ttatcgatgg tgaactgtat gaaatgctca gcgcgcgtcc tctgggcgta
     8641 cagccgcgcg gtggggtaat ctctccacag ccagccttta tgcaactggt gcgtcaggag
     8701 ctgcaggcga aattaggcga taaggtaaaa gatctctccg gcgtgaagat cttcactacc
     8761 tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat tccggcactg
     8821 aagaaacagc gtaagttgag cgatcttgaa actgcaattg tggtcgtcga ccgctttagt
     8881 ggtgaagttc gtgcgatggt gggaggttct gagccgcagt ttgcgggcta caaccgtgcg
     8941 atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct gacggcttta
     9001 agccagccga aaatctatcg cctgaacacg tggattgccg atgcgccaat cgcgctgcgt
     9061 cagccgaatg gccaggtctg gtcaccgcag aacgatgacc gtcgttatag cgaaagcggc
     9121 agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt aaatctgggg
     9181 atggcgctgg ggctgcctgc agttacggag acctggatca aactgggcgt gccgaaagat
     9241 cagctgcatc cggttccggc gatgctgctg ggggcgttga acttaacgcc aatcgaagtg
     9301 gcgcaggcat tccagaccat cgccagcggc ggtaaccgtg caccgctttc tgcactgcgt
     9361 tcggtaatcg cggaagatgg caaggtgctg tatcagagct tcccgcaggc agaacgcgct
     9421 gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt acaacgtggt
     9481 acgggtcgtc aacttggggc gaaatacccg aacctgcatc tggcagggaa aacagggact
     9541 accaacaata acgtagatac ctggtttgcg ggcattgacg gcagcacggt gaccatcacc
     9601 tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gtgccagcgg ggcaatgtct
     9661 atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt tccgccagaa
     9721 gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg tggcatgcgt
     9781 gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagcga gatgcagcag
     9841 cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc gcaacagcaa
     9901 cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa ggatatgttt
     9961 ggtagtaatt aacatctaag cgtgaaatac cggatggcga attgccatcc ggtaaaataa
    10021 catcccacct aaagtattaa ccctttcttt tcatctggtt gtttattaac ccttcaggaa
    10081 cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc ttatctttat
    10141 aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc aatggcgcgt
    10201 tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt agccacagcg
    10261 gttagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga cactatcacc
    10321 gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac tattgcggcg
    10381 aagcactctg ctactgcgac taaaacggat acaccaattg aaaaaacgcc gcagtctatt
    10441 tcggttgtga caaatgaaga gatgcagatg cgtcaatttc agtctgtaaa agaagcatta
    10501 ggttatacac ctggcgttac ggttagcagt cgtggtgctt ctaatacata tgattttgtt
    10561 atcattcgtg gtttctcatc tgttggtctg aatcaaaata attaccttga tggactaaaa
    10621 cttcagggca acttttataa cgatgctgtg attgatcctt acatgctcga gagggttgaa
    10681 ctgatgcgtg ggccgacgtc tgttctctat ggaaaaagta atcctggtgg gattatttct
    10741 atggtgagta agcgtccgac cactgaaccc ctgaaagaaa ttcagtttaa aatggggacg
    10801 gataatctgt ttcagaccgg attcgatttc agtgatgcac tggatgataa cggtgagttc
    10861 tcttaccgtt tgacgggcct cgctcgctca acaaacgaac agcagaaaaa ctctgaatct
    10921 cagcgttata ccattgctcc atcattctca tggcgtccag acgacaaaac caattttact
    10981 ttcctgtcct atttccagaa tgaaccagaa acgggttatt acggttggtt gccgaaagag
    11041 gggaccgttg agccattgcc taatggtaag cgtctaccga ctgacttcaa tgaaggtgca
    11101 tcgaataata catactcccg taaccagaaa atggtgggat atagttttga acatggtttt
    11161 aatgacacct tcaccgtgcg tcagaatctg cgtttcagtg aaatgaaaac ctcacagaaa
    11221 agtgtttatg gcacagggat tgctggtgat ggtcataccc taaaccgcgg gacagtggtg
    11281 gataatgagc gtctacaaaa ctttagcgtt gatacccaac ttgaaagtaa gtttgctaca
    11341 ggtgacgtcg agcatacttt gctgacaggg gtagacttca tgcggatgcg taatgatatc
    11401 aatgccagct ttggatccgc accatccatc gatctttata acaaatatca ccctgaatac
    11461 tttgcatttg gtagcgcaga gccataccag atgaatgaaa gcaaacaaac aggtatttat
    11521 gttcaggatc aggcggaatg gaataaatgg gtattcactc tggggggacg ctacgattgg
    11581 tctaagcaag cgaccactgt tcgtgaaaac tcttatacgc cgactgaagg ttatattgag
    11641 cgcaatgatc atcagttcac ctggcgcggt ggtgtaaatt acttattcga taatggtatt
    11701 tcaccttact ttagctatag ccagtccttt gaaccgagtg ctttcgatct gtggagcaac
    11761 ccgcgcattt cctataagcc atcgaaaggt gaacagtatg aagctggcgt aaaatatgtt
    11821 ccgaatgata tgccggtcgt tgttacgggc gcagtctatc aattgacgaa aacaaataac
    11881 ctgacagcag acccaacaaa cccattagcg caagtcccag caggtgagat tcgcgctcgt
    11941 ggtgtggaac ttgaagcaaa agcggcgtta aatgccaata ttaacttgac ggcttcttat
    12001 acctacaccg atgcggaata caccaaagac accaatctca aaggtaaaac tccagaacaa
    12061 gtaccggagc atatggcatc tctctggggg gattatacct tcaatgaagg gccgctttct
    12121 ggtttaacat tgggaacagg tggtcgtttt attggttcca gctatggtga tccggcaaac
    12181 acttttaaag tgggtagcac agctgtaatg gatgctgttg taaaatatga tctggcacgc
    12241 tttggtatgg cgggatccag ccttgctgtg aacgtcaaca atttgctcga tcgtgagtat
    12301 gttgccagtt gcttccagac ttatggctgc ttctggggcg cagaacgtca ggtcgttgca
    12361 accgcaacct tccgtttcta atttctcttt tgggacacgg atttccgtgc ccatttcaca
    12421 agttggctgt tatgcaggaa tacacgaatc attccgatac cacttttgca ctgcgtaata
    12481 tctcctttcg tgtgcccggg cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg
    12541 ggaaagtgac cggtctgatt ggtcacaacg gttctggtaa atccactctg ctcaaaatgc
    12601 ttggtcgtca tcagcctccg tcagaagggg agattcttct tgatgcccag ccgctggaaa
    12661 gctggagcag caaagcgttt gcccgcaaag tggcttattt gccgcagcag cttcctccgg
    12721 cagaagggat gaccgtgcgt gaactggtgg cgattggtcg ttacccgtgg catggcgcgc
    12781 tggggcgctt tggtgcggca gatcgcgaaa aggtcgagga agctatctcg ctggttggct
    12841 taaaaccgct ggcgcatcgg ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt
    12901 ggatcgccat gctggtggcg caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg
    12961 cgctggatat cgcccaccag gttgatgtgc tggcgctggt gcaccgttta agtcaggaac
    13021 gtggcctgac ggtcattgcc gtgttgcacg atatcaatat ggcggcacgc tactgtgatt
    13081 atctggtcgc cctgcgcagt ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc
    13141 gcggcgaaac cctcgaaatg atttatggca tcccgatggg tattttgccg catccggcgg
    13201 gtgctgcacc tgtgagtttt gtttattgat gagcggctta cctcttattt cgcgccgtcg
    13261 actgttaacg gcgatggcgc tttctccgtt gttatggcag atgaataccg cccacgcggc
    13321 ggctattgac cccaatcgta ttgtggcgct ggagtggttg ccggtggaat tactgctggc
    13381 gctcggcatc gtgccttacg gcgtggcgga taccatcaac tatcgcctgt gggtcagcga
    13441 accaccattg ccggactcag tgattgacgt cggtttgcgt acagaaccta accttgaact
    13501 gctgaccgaa atgaaaccgt cgtttatggt ctggtcggca ggatatggcc cttcatcaga
    13561 aatgctggca cgtattgcgc cgggtcgcgg atttaacttc agtgacggca aacagccgtt
    13621 ggcgatggcg cgtaaatcgc tgacggaaat ggcagattta cttaacctgc aaagcgcagc
    13681 ggaaacgcat ttagcgcaat acgaagactt tatccgcagc atgaaacccc gctttgtgaa
    13741 gcgtggtgca cgcccgttac tgctgacgac gcttatcgat ccgcgccata tgctggtctt
    13801 cggtccaaac agcttgttcc aggaaattct cgatgagtac ggcatcccaa atgcctggca
    13861 aggggaaacc aacttctggg gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa
    13921 agacgttgat gtgctctgtt ttgatcacga caacagcaaa gacatggatg cgctaatggc
    13981 aaccccgctg tggcaggcca tgccgtttgt ccgcgccgga cgctttcagc gcgtacctgc
    14041 agtctggttt tatggtgcga cactctcggc aatgcacttt gtgcgcgttc tggataacgc
    14101 catcggaggt aaagcgtgag taaacgaatt gcgctttttc cggcgttatt gctggcgctg
    14161 ttagtgattg tcgctacggc gctcacctgg atgaacttct cgcaggcgct gccgcgtagc
    14221 cagtgggcgc aggctgcctg gtcgccagac atcgatgtca tcgagcagat gatttttcac
    14281 tacagcttgt tgccgcgtct ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg
    14341 ggcgtgctgt ttcagcaagt gctgcgtaac ccgctggcgg agccgacgac gcttggcgtt
    14401 gctacaggcg cgcaactggg aattaccgtc accacacttt gggcgatccc aggtgctatg
    14461 gcgagccagt ttgccgcact ggtgggcgct tgtgtcgttg gcttaatcgt ctttggcgtc
    14521 gcgtggggga aacggctttc gccggttacg cttattctcg cggggctggt ggtgagcctt
    14581 tattgcgggg caattaatca gctactggtt atcttccatc acgaccagct gcaaagcatg
    14641 ttcctgtgga gcaccggaac gctgacgcaa accgactggg gcggcgttga gcgtttatgg
    14701 ccgcagttgc tgggcggcgt gatgttgacg ttattgctac ttcgcccgtt aaccctgatg
    14761 gggcttgatg atggcgtggc gcgcaatctc gggctggcct tgtcgctcgc gcgtctggcg
    14821 gcgctgtcgc tggcgatagt catcagtgcg ctgctggtga atgcggtggg gattatcggc
    14881 tttatcggtt tgttcgcgcc gctgctggcg aaaatgctgg gggcgcggcg tttgttgcca
    14941 cggttgatgc tggcgtcgct gattggggcg ctgatcctct ggctttccga tcaaatcatc
    15001 ctctggctga ctcgcgtgtg gatggaagtg tccaccggtt cggtcactgc gttgatcggt
    15061 gcgccgctgc tactgtggct gttgccgcgt ttacgcagca ttagcgcgcc ggatatgaag
    15121 gtcaacgatc gtgtcgcggc tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc
    15181 gtgctgctgt tgatggctgt ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg
    15241 acgtgggcga gcggggcgtt gctcgaggat ttgatgccct ggcgctggcc gcgaattatg
    15301 gcggcgctgt ttgcgggcgt catgctggcg gtggcgggct gtattattca gcggctgacc
    15361 ggaaacccga tggcaagccc ggaagtgctg gggattagct ctggcgcggc gtttggcgtg
    15421 gtgttaatgc tgtttctggt gccgggtaat gcctttggct ggctgttacc cgcgggcagt
    15481 atcggggcgg cggtgacgct gttgatcatt atgatcgccg ccggccgcgg tggattttcc
    15541 ccacaccgta tgttactggc ggggatggcg ttaagtaccg cattcaccat gcttttgatg
    15601 atgttgcagg cgagcggtga cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg
    15661 acctacaacg cgaccgatgc gcaggtctgg cgtaccggaa ttgtgatggt gattttgctg
    15721 gcgattaccc cgctgtaccg ccgctggctg accattttac cgctgggtgg tgataccgca
    15781 cgagccgtag gaatggcgct gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc
    15841 ctgacggcga ccgcgacgat gactattgga ccgttgagtt ttgttggttt aatggcaccg
    15901 catattgcgc ggatgatggg ctttcgacgg acgatgccac acatcgtaat ttcggcgctg
    15961 gtgggtggtt tactgctggt attcgctgac tggtgtgggc ggatggtgct gtttccattc
    16021 cagatcccgg cggggctgct gtcgaccttt atcggcgcgc catattttat ctatttgctg
    16081 agaaagcaga gccgttaatt tttccatata acggccctgt acgcctggac ggataaagcg
    16141 tttacgcgac aacggatgcc cgatgcgacg ctggcgcgtc ttatcgggcc tacaaacggc
    16201 cccgaatcgt aggtcggata aggcgttcac gccgcatccg acaaaccatg ctggatcaca
    16261 acttcgcaaa cacccgacgt gcagcatcga tggtgttatt gatatcttcc atgctgtgcg
    16321 ccacggacat aaagcccgct tcaaacgctg acggtgccag gtaaacacct tcgtccagca
    16381 tcatatggaa gaaacgctta aagcgttcca cgtcacaggc catcacatcc tgatagcacg
    16441 tcacggactc ggcgtcggta aagaaaatac cgaacatgcc gccaacgtgg ttaacgacca
    16501 gcggaattcc ggcttcttct gccgcttcca gcagaccttc tgccagacgt gttgtcagct
    16561 catccaacgt ttcgtgaacg cccggttgcg cgacttcatt cagacaggcg aaaccggctg
    16621 ccatcgcaat tgggttaccg gaaagcgtac ccgcctgata gaccggaccc gtcggggcca
    16681 gcgcatccat tacatcacga cgaccaccga atgcgcctac cggcattcca ccgccgatga
    16741 ttttgcccag gcaggtgaga tccggttcca caccgtaata atcctgtgcg ccagccagtg
    16801 ccacgcggaa gccggtcatt acttcatcga tgatcagcaa tgcgccaaat tcgtcgcaca
    16861 gcgcacgcag acctggcagg aagtctggca gcggtggaac acagttcata ttgcctgcca
    16921 ccggctcgat gataatacag gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag
    16981 aagccagatc gttataagta caggttaagg tatgtttggc gaaatctgcc ggaacgcccg
    17041 gcgagtttgg ctggcctaac gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag
    17101 cgtgaccgtg gtaacaccct tcaaatttaa tgattttgtc gcgaccggta aaaccacggg
    17161 ccagacggat ggcgctcatc gtcgcctcgg tgccggagtt caccatgcgc accatatcca
    17221 tggtcgggac caattcggtc accagttgcg ccattttcac ttccatttcg gttggtgcac
    17281 caaagcttaa accacgctcg gcggcttcaa tcacggcatt gcggattgcc ggatggttat
    17341 ggcccagcac catcggcccc caggaaccga cataatcgat ataggctttg ccatcaacat
    17401 cgtacagata agcgccgtcc gctttttcga taaacagtgg agtgccgccc acgccagtaa
    17461 aggcgcgaac aggggagttc acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat
    17521 tttcagactt actcatggag ggttcctgat tcgtagaaaa agtgaatggc tgctattcta
    17581 tgttattcat aacaagttaa atactcgtca aacatcaggc tgcttgtact ggtgcaatcc
    17641 tgatttctta gagtataaaa gttttgtgca tttgaaacat tacgctttgc aaaggatttt
    17701 catggaacgt gcgagtaaaa tgccgtcatc ttatttgtat gaccaataag tgatcattgg
    17761 atgaaaactg atactccctc tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa
    17821 ctgattcgcc aacttcttga gcgcgataaa accccgttag ccattttgtt tatggcggca
    17881 gtcgtcggca cgcttgttgg gctggcagcg gttgcttttg acaaaggtgt cgcctggttg
    17941 cagaaccaac gtatgggggc gctggtacat actgctgata attatccgct tctgttaacc
    18001 gtcgcttttc tctgttcggc ggtgctggcg atgtttggct actttttggt gcgcaaatac
    18061 gcgccggaag caggtggttc ggggatcccg gaaattgaag gggcgctgga agatcaacgt
    18121 cccgttcgct ggtggcgtgt attgccggtg aagttctttg gcgggctggg gacactcggc
    18181 ggaggcatgg tgttggggcg cgaagggcca accgtgcaga tcggcggtaa cattggccgt
    18241 atggtgcttg atattttccg cctgaaaggt gacgaagctc gccatacgct gctggcaacc
    18301 ggtgctgctg cggggctggc tgcggccttt aacgcgccgc tggcgggtat tttgtttatt
    18361 atcgaagaga tgcgtccgca gtttcgctat acgttaattt cgataaaagc ggtatttatt
    18421 ggcgtcatta tgtcgaccat tatgtaccgg atttttaatc atgaagttgc gttgattgac
    18481 gtcggtaaac tttctgatgc gccgcttaat acgctgtggc tttatctgat cctcggtatt
    18541 atttttggca ttttcggccc tatttttaat aaatgggtgc tggggatgca ggatttgctg
    18601 caccgtgtgc acggcggcaa tattaccaaa tgggtactga tgggcggtgc gattggcggt
    18661 ctgtgtggat tgctggggtt tgtggcacca gcaacgtcgg gcggcggttt taacctgatt
    18721 cctatcgcta ccgcggggaa tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg
    18781 gtcattacca ccttactctg cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg
    18841 ctggcgctgg gtactgtgct gggaaccgct ttcggaatgg ttgccgttga gctgtttccg
    18901 caatatcacc ttgaggcggg gacgtttgct attgccggaa tgggggcatt actggcggca
    18961 tctattcgcg cgccgttaac ggggatcatt ctggttctgg agatgaccga taactaccag
    19021 ctcattttgc caatgattat taccggtctt ggcgcaacac tattagcgca atttaccggc
    19081 gggaaaccgc tatactcggc gattcttgcg cgcacgctgg caaaacagga agctgagcaa
    19141 ctggcgcgaa gcaaggccgc atcagccagc gagaatactt gaacgaaata ccagggtatt
    19201 agataatggc gattattatt gggttagaat ttgcccaatt gccgatgtcg tttggagcaa
    19261 aatatgagtg atgacgtagc actgccgctg gagtttaccg acgcagcagc caacaaagtt
    19321 aaaagcctga tcgctgacga agataacccg aatctgaaat tacgcgtgta tatcaccggt
    19381 ggcggttgca gcggcttcca gtatggtttc acctttgatg atcaggtgaa cgaaggcgat
    19441 atgaccatcg aaaaacaggg cgttggcttg gtggttgatc cgatgagcct gcaatatctg
    19501 gtcggcggtt ccgttgacta taccgaagga ctggaaggtt ctcgtttcat cgtgaccaac
    19561 ccgaacgcga aaagcacctg cggttgcggt tcttccttta gcatctaatc tgttgttcat
    19621 gtgccggata aactatccgg cgcatcttct ctcttcaacg cccattctca tccagcgcaa
    19681 acgtcggtag cttaagatgc caacgaatag ccgccagccg aatcaatagc gtcacgacca
    19741 tgcccatcat actggctgtt tccagtggta cggaaaatgt gtaataagcc gtagcgtgga
    19801 caataccgcc gataatacag gcagttgcgt agatttctgt acgtaaaatc atggggatct
    19861 cgcgggccag tacatcacga atgatcccgc cgccaacgcc agtaatgacg accatacaaa
    19921 ccgcgattaa cggaccgact tccgcattaa aggctttatt cacgccaatg ccgacaaaca
    19981 ccgccagacc aacggcgtcc agcaccggca acatccattt tggtaagcgt cttggctggc
    20041 gcaccagcac gatggtcagc atgctggtta ccattgcaac gaccagatcg gtgggatctt
    20101 tcacccaaaa taccgggccg tgatccagcg ccatgtcgcg aattgtcccg ccgcctacag
    20161 cggtaaccac gcccagtacc agaacaccaa aagggtccat acgcaatttt ccggctaaca
    20221 aaacgccgga gatggcaaat accgctgtgc cgactatatc cagccaatag acgagcattg
    20281 ttcaatcccc actgagcacc taaaaaggtc aggcgctaat ctacctgtga aagcgcatta
    20341 cagagctgtt gtgcagcgag gataatacgt gggcttgcac gttcaaacca gtcactcgtg
    20401 agaggaataa cgggaatttt gagctgttca ccccagtatt gtttgatttt aggaatttgg
    20461 tccggtccgc ctgtaatgac aatcgcctgt ggcgagcgtg ctaacacctg ttcgcggcta
    20521 acttgcggcc agggaacccg gctgtcttta aagatgtttt ctccgccaca aacttcgagt
    20581 acctggtttt gaatcgactc ttttccactg gtaaatggcg gattaatgcc gaattgcaga
    20641 aaaacacgtt ttttaggttt atcagcatat tgcgctttca attgcgcgta ctgatccagc
    20701 agggattgcg cggcttgttc ggccttatcc ggttgcggac tccagggggc caattgacgt
    20761 aacgcattgg caatttgttc aatgcttgtc gcatcgaccc acatcacttt tattcccagc
    20821 gaagccagct ggtcaacctg ccgctcggca ttacctccac gccaggcaat caccagatcg
    20881 ggtttcagcg cgacaatgcg ttccagattc atcccctgcc aggtggaaac ctgctcaatc
    20941 ttttgcgctt gtggaggata gtcggaatag ctgctgaccc caaccggcgt gatcccggcg
    21001 gcaaaggcaa gttcagtgtt ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc
    21061 cacagtggcg caagaaaaga cagggcgacc agcgccctga acagtgactt agccatgtgc
    21121 cagtttctgc accagtgact caaccatcag gctggactgt ttagcggcaa cagccaggaa
    21181 ctcatcgaag ctaagatgag actgttgatc ggccacgtcg gagatggcgc gtacgacaac
    21241 aaacgggacg ttgaaattgt ggcagacatg ggcgattgcc gtcgcttcca tctctacagc
    21301 aatggcctgt gggaagttgt ggcggatttt cgccagacca acagaaccgt tgatgaaagc
    21361 gtcgccgcta acaatcaggc cacgtacagc gttaagattc agttcggcaa tgcaggcctc
    21421 agcggcagcg atcagtttat cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc
    21481 gtattcataa ccaaatgccg tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac
    21541 gatatcgccc actttcagcg ttggtgccag gccaccggca gaaccggtgt taataatcac
    21601 atctggcttg cagtgttcca acagcagagt ggcacccagc gccgcagcga ctttaccgat
    21661 gcccgatttc agaagcgcaa cctcggttcc attcagttgg ccggtataga tttcgcaacc
    21721 gccgagactg atagtttgac ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc
    21781 cattgcacca atgatgccga ttttcataga tttactcgcg ataagcccga tttgaaagca
    21841 tagtttacca tgcgcttacg gggaagcgta tttctcacgc gggagaggac atggcacaga
    21901 ttgatttccg aaaaaaaata aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa
    21961 ccgaacatga gatcctgcgg atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg
    22021 caattcgtcg tctgcaacaa aagacccagg tttttccact ggagcgcaat gccgccgtgc
    22081 gcacgcgtct tacccactcg atggaagtcc agcaggtggg acgctacatc gccaaagaaa
    22141 ttttaagccg tctgaaagag cttaaattgc tggaagcata cggcctggat gaactgaccg
    22201 gcccctttga aagcattgtt gagatgtcat gcctgatgca cgatatcggc aatccgccgt
    22261 ttggtcattt tggcgaagca gcgataaatg actggtttcg ccaacgtttg cacccggaag
    22321 atgccgaaag ccagcctctg actgacgatc gctgcagcgt ggcggcacta cgtttacggg
    22381 acggggaaga acagcttaac gagctgcggc gcaagattcg tcaggactta tgtcattttg
    22441 aggggaatgc acaaggcatt cgtctggtgc atacattgat gcggatgaat ctcacttggg
    22501 cacaggttgg cggtatttta aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg
    22561 agacacatca ctatttaatg aaaaagccgg gttattatct ttctgaagaa gcctatattg
    22621 cccggttgcg taaagaactt aatttggcgc tttacagtcg ttttccatta acgtggatta
    22681 tggaagctgc cgacgacatc tcctattgtg tggcagacct
//
LOCUS       DAZSBF010000052        22334 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZSBF010000052 DAZSBF010000000
VERSION     DAZSBF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.053"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(133..3282)
                     /gene="acrB"
                     /locus_tag="ACHXWD_001563"
     CDS             complement(133..3282)
                     /gene="acrB"
                     /locus_tag="ACHXWD_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR7620507.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3305..4498)
                     /gene="acrA"
                     /locus_tag="ACHXWD_001564"
     CDS             complement(3305..4498)
                     /gene="acrA"
                     /locus_tag="ACHXWD_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR7620508.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4640..5287
                     /gene="acrR"
                     /locus_tag="ACHXWD_001565"
     CDS             4640..5287
                     /gene="acrR"
                     /locus_tag="ACHXWD_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR7620509.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5415..8777
                     /gene="mscK"
                     /locus_tag="ACHXWD_001566"
     CDS             5415..8777
                     /gene="mscK"
                     /locus_tag="ACHXWD_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR7620510.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8838..8936
                     /locus_tag="ACHXWD_001567"
                     /pseudo
     CDS             8838..8936
                     /locus_tag="ACHXWD_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8989..9150)
                     /gene="rsmS"
                     /locus_tag="ACHXWD_001568"
     CDS             complement(8989..9150)
                     /gene="rsmS"
                     /locus_tag="ACHXWD_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR7620511.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(9164..9691)
                     /gene="priC"
                     /locus_tag="ACHXWD_001569"
     CDS             complement(9164..9691)
                     /gene="priC"
                     /locus_tag="ACHXWD_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR7620512.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9761..10138
                     /locus_tag="ACHXWD_001570"
     CDS             9761..10138
                     /locus_tag="ACHXWD_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR7620513.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10291..10842
                     /gene="apt"
                     /locus_tag="ACHXWD_001571"
     CDS             10291..10842
                     /gene="apt"
                     /locus_tag="ACHXWD_001571"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR7620514.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10971..12896
                     /gene="dnaX"
                     /locus_tag="ACHXWD_001572"
     CDS             10971..12896
                     /gene="dnaX"
                     /locus_tag="ACHXWD_001572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR7620515.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    12229..12293
                     /gene="dnaX"
                     /locus_tag="ACHXWD_001572"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12949..13278
                     /locus_tag="ACHXWD_001573"
     CDS             12949..13278
                     /locus_tag="ACHXWD_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR7620516.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13278..13883
                     /gene="recR"
                     /locus_tag="ACHXWD_001574"
     CDS             13278..13883
                     /gene="recR"
                     /locus_tag="ACHXWD_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR7620517.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13993..15867
                     /gene="htpG"
                     /locus_tag="ACHXWD_001575"
     CDS             13993..15867
                     /gene="htpG"
                     /locus_tag="ACHXWD_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR7620518.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16048..16692
                     /gene="adk"
                     /locus_tag="ACHXWD_001576"
     CDS             16048..16692
                     /gene="adk"
                     /locus_tag="ACHXWD_001576"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR7620519.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            16824..17786
                     /gene="hemH"
                     /locus_tag="ACHXWD_001577"
     CDS             16824..17786
                     /gene="hemH"
                     /locus_tag="ACHXWD_001577"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR7620520.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17783..18742)
                     /gene="aes"
                     /locus_tag="ACHXWD_001578"
     CDS             complement(17783..18742)
                     /gene="aes"
                     /locus_tag="ACHXWD_001578"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR7620521.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18894..20198
                     /gene="gsk"
                     /locus_tag="ACHXWD_001579"
     CDS             18894..20198
                     /gene="gsk"
                     /locus_tag="ACHXWD_001579"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR7620522.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20331..22007)
                     /gene="ybaL"
                     /locus_tag="ACHXWD_001580"
     CDS             complement(20331..22007)
                     /gene="ybaL"
                     /locus_tag="ACHXWD_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR7620523.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22245..>22334)
                     /locus_tag="ACHXWD_001581"
     CDS             complement(22245..>22334)
                     /locus_tag="ACHXWD_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620524.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         5438 a         5918 c         5857 g         5121 t
ORIGIN      
        1 tttatgaatt ttatccgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc
       61 aattactcct taatgttcgt aggttatgca taaaaaaggc cgcttgcgcg gccttagtga
      121 ttacacgttg tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg
      181 gctaaagcgg cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt
      241 tgcggtcacc atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc
      301 accagtactg ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat
      361 cggacgtaaa cgcatccgca ctgcatcaag cgtcgcttca atcagacctt taccttcttt
      421 atccatcaag tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat
      481 ggttgtgagc aggcctacct ggaagtaaac gtcattggtc aggccacgga aggtggcagc
      541 cagcaacgca ccgataaccc ccagcggaac gaccagcata acggagaacg gaatcgacca
      601 gctctcgtac agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga
      661 aggtgcctgg ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac
      721 accggtaggc agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt
      781 acccggtgcc gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg
      841 cggcgaaccg tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc
      901 agcagcacga acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga
      961 catgacgtaa actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc
     1021 ccatgcagcg cccagagagg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt
     1081 ttcctggtcg atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct
     1141 ggtcagcata tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag
     1201 tttttcgtga ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc
     1261 cagttccacg attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa
     1321 agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc
     1381 ccagtccttc aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa
     1441 gccgttaacg gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt
     1501 tacctcattg agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat
     1561 ggtcataaat acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag
     1621 ataggccatg ccgaccacga tgatcagata cagcaccagg taacgccccg tactgcgcag
     1681 aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca
     1741 gccgaagaag ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt
     1801 ggcacaaaga gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac
     1861 aatggtaata gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg
     1921 tacgaatacc gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat
     1981 cgacttacgg gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt
     2041 ttctaccaca acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat
     2101 tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac
     2161 caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat
     2221 aaccaggaac acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat
     2281 tttcacgaac ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg
     2341 ttccatcttc gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt
     2401 cgccagcttg atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta
     2461 gttctcacca cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg
     2521 attcactttc agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat
     2581 aatagaggcg ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc
     2641 aacctgggcg ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt
     2701 gttcagctca ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac
     2761 atcacccacg cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga
     2821 gatatcctcc tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct
     2881 gctggatgat ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat
     2941 cgccagctgc agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc
     3001 aaaggtcagg gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag
     3061 gttatcgata ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt
     3121 cgcatcagcg ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata
     3181 ttgcgccacc ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat
     3241 cacccacgca aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt
     3301 taagttaaga cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag
     3361 cggtaacttc ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta
     3421 ctacgcgatc gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc
     3481 ttgcaacgat cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat
     3541 caccacgcgg cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc
     3601 cttcttccag acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga
     3661 agatagcgcg tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca
     3721 gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc
     3781 cgttctcttg tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc
     3841 tggactgggt cacatcaagg tagatcggat caagttgctg cacggttgcc agcgcagtcg
     3901 cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga tttaccaatg cgaccgctaa
     3961 tcggagaggt gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg
     4021 ccgcagttac cgcagcattc gcctgttgcg catcagccag agcctgatcg tactcttgct
     4081 tactgatgta ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat
     4141 tggctgcagc ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg
     4201 tcgcaggatc aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat
     4261 tacgcttcag gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg
     4321 tgcgacccgg aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa
     4381 cggcgggcat ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta
     4441 agctgcctga gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat
     4501 gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc
     4561 tgcgtttata ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg
     4621 cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca
     4681 acacatcctc gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct
     4741 gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga
     4801 caagtcggat ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga
     4861 gcttgagtat caggcaaaat tccctggcga tccactctca gtattaagag agatattaat
     4921 tcatgttctt gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt
     4981 ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg
     5041 tctggaaagt tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt
     5101 gcctgcggat ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct
     5161 gatggaaaac tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta
     5221 cgtcgccatc ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa
     5281 cgaataaccc tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg
     5341 gttcactgcg tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga
     5401 aaccttcatt catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt
     5461 cagcatttat tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt
     5521 catcgaatgg tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata
     5581 aacaaaaaga tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag
     5641 ccaccctcga taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg
     5701 ctgaagcgcc ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg
     5761 ataacgacga agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc
     5821 gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca
     5881 gccagctggt ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt
     5941 cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct
     6001 tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg
     6061 accagcagcg taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac
     6121 gtgattacgt gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag
     6181 cggtaaacag caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg
     6241 atgaagccgc gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc
     6301 agttaagtca gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca
     6361 ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga
     6421 ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc
     6481 tgccctcggc ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac
     6541 agtttgaagt taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac
     6601 tggaagaagg tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg
     6661 ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga
     6721 tggccattaa cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat
     6781 ccatcctgac tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga
     6841 tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact
     6901 gggaaaaagc ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga
     6961 ttgccgggct gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt
     7021 ccgctgtggg ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg
     7081 acctgatccg cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga
     7141 ccatgcagct caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct
     7201 ggctggtgtt tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact
     7261 tcggcatgcc ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg
     7321 cgttgctgcc tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg
     7381 atgatgtgct ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat
     7441 ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctggtcacca
     7501 ttaccgtgct gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct
     7561 acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga
     7621 acttgctcta ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc
     7681 gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc
     7741 cggaagaacc caccattgca ctggaacagg ttaaccagca gacgctgcgt attaccatgt
     7801 tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg
     7861 tgttcagcta tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg
     7921 cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg
     7981 cctgggcgtt gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga
     8041 atatgcgtca gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg
     8101 ttggtgcgat gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg
     8161 ccgcagcatt atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct
     8221 ccggtttgat cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta
     8281 gcttctcggg gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc
     8341 gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt
     8401 tgactgacac tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg
     8461 aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac
     8521 caatgccgga agtcttcttt acggcgtttg gtgccagcac gttggatcat gagctgcgtc
     8581 tgtatgtgcg tgaactgcgt gaccgtagtc gtactgtcga tgagctgaac cgtactatcg
     8641 atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc
     8701 acaacgagaa gggcgatgag gtgacggaag tcaaacgcga ctacaaaggc gatgacccga
     8761 cgccagcggt agggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca
     8821 aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca
     8881 agaattcaat gtattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt
     8941 tgcgtttgcc cacaatctta ggggacattt agcggcccca tttatttctc acttttccgc
     9001 cttatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca gcgcgcgcag
     9061 cacggtgcgg gctgggatct gattttcttc cagcagcaca atcaaatcga cagccagttt
     9121 gacatcgtca ggggcatttt caagtgacat actctctcca ttgctagcgg gttaagcgcg
     9181 ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg
     9241 cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg
     9301 ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt
     9361 tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg
     9421 aggcttcacg cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt
     9481 gcggcagctg ttgctgctca accgcatgac aaagcgcagc cagattatcg cccgcctcgt
     9541 cgagacaagc ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac
     9601 ttagcgtggc gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt
     9661 ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt
     9721 tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat
     9781 catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc
     9841 gacgacgccg tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca
     9901 cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca
     9961 tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat
    10021 ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg
    10081 tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag
    10141 cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac
    10201 aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa acgcgccgtg
    10261 agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat
    10321 ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat
    10381 gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag
    10441 cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt
    10501 ggcgctccgg tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa
    10561 ctgccgcgtg aaaccatcag tgaaacttac gacctggaat acggcaccga tcagctggag
    10621 atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca
    10681 accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct
    10741 gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag
    10801 ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc
    10861 tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg
    10921 ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc
    10981 aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg
    11041 tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt
    11101 ccggcacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact
    11161 gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc
    11221 aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata
    11281 cccgcgacct gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc
    11341 tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg
    11401 aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg
    11461 tgacgatttt gtcgcgctgt ttgcaatttc atctcaaggc gctggatgtc gagcaaattc
    11521 gccatcaact tgagcacatc ctcaacgaag aacatatcgc tcacgagccg cgggcgttgc
    11581 aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg
    11641 cgattgccag cggtgacggc caggtttcaa cccaggcagt cagtgcgatg ctgggtacgc
    11701 ttgatgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac ggcgagcgcg
    11761 taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg
    11821 aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca
    11881 acgacatggc cgccattgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg
    11941 atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg
    12001 accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc
    12061 cgctgcctga gccagaagtg ccacgccagt cctttgcacc cgtcgtgcca acggcagtaa
    12121 tgacgccaac ccaggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc
    12181 cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagtgcgtg cagggagcaa
    12241 ccaaagcaaa aaagagtgaa tcggcagccg ctacccgcgc gcggccggtg aataacgctg
    12301 cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc
    12361 tggaaaaagc gtcagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc
    12421 agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa
    12481 cgccggaact ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac
    12541 aggtgagcca actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag
    12601 aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc
    12661 gcggtgcaca gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac
    12721 tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga
    12781 tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga
    12841 ccctgcgtcg gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt
    12901 aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa
    12961 ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg
    13021 caggaagaga tcgcgcagct ggaagtcacc ggcgaatctg gcgcaggtct ggtaaaagtg
    13081 accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac
    13141 gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt
    13201 gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc
    13261 tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc
    13321 gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc
    13381 gcgatcgtag cggcgggatg cgtttggcgc aggcgctcac ccgggcgatg tcggaaatcg
    13441 gccactgcgc cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc
    13501 cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca
    13561 ttgagcagac ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc
    13621 tggacggcat cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg
    13681 aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta
    13741 actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg
    13801 taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac tcccttgccg
    13861 ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct cgcttgaaat
    13921 tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg
    13981 aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt
    14041 ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc
    14101 tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg
    14161 tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg
    14221 accatctctg ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact
    14281 atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac
    14341 agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa
    14401 gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg
    14461 gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc
    14521 accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc
    14581 atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa
    14641 gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct gtggactcgt
    14701 aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat cgcccacgac
    14761 tttaatgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc
    14821 ctgctgtaca ttccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc
    14881 ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt catgccgaac
    14941 tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt
    15001 gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg
    15061 ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa
    15121 cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc
    15181 gccaaactgc tgcgttttgc ttctacccat accgattctt ctgcgcagac cgtatctctg
    15241 gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat caccgcagac
    15301 agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa
    15361 gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac
    15421 ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa
    15481 gttgatgaga gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgtgtgaaa
    15541 gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca
    15601 gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg
    15661 gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg
    15721 gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt agaactgctg
    15781 ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt
    15841 cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca
    15901 tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg tttcagcctc
    15961 aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa
    16021 tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg
    16081 gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact
    16141 ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac
    16201 attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc
    16261 attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg
    16321 caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta
    16381 ccggacgaac tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt
    16441 gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa
    16501 gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac
    16561 catcagatga ctgcaccgct gatcggctac tacttcaaag aagcggaagc gggtaacacc
    16621 aaatacgcga aagttgacgg caccaagtcg gttgctgaag ttcgcgctga tctggaaaaa
    16681 atcctcggct aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca
    16741 ggcaatacct ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca
    16801 agttgaatcg ataagaggcg gtaatgcgtc agactaaaat cggtatcctg ctggcaaacc
    16861 tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa
    16921 gcgacagacg cgtggttgat acctcacggt tgttatggtg gccattgctg cgcggcgtta
    16981 ttttgccgct gcgctcgccg cgtgtggcga agctgtacgc ctcggtctgg atggaaggtg
    17041 gctcgccgct gatggtttac agccgccagc aacagcaggc gctggcacaa cgtttaccgg
    17101 agacgcccgt agcgctggga atgagctacg gctcgccatc actggaaagc gccgtcgatg
    17161 aactcctggc agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct
    17221 gttctacggt cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca
    17281 ttccggggat atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg
    17341 cgaacagcgt acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt
    17401 atcatggcat tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca
    17461 caacgactcg cgaactggct tccgcactgg ggatggcacc ggaaaaagtg atgatgacct
    17521 ttcagtcgcg ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa
    17581 tgctcggaga aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt
    17641 gtctggagac gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg
    17701 ggaaaaaata tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg
    17761 ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa
    17821 gagcctcgtc ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg
    17881 ggtagagttt gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc
    17941 tgtcatccag cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag
    18001 tgagatcatt attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat
    18061 acgcctcttc atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca
    18121 gacgacgagt cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa
    18181 ttttaccgca atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg
    18241 cacctgcgga atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct
    18301 gctggtggaa ataacaacaa gcagccacaa tttcctctat cgcttgcgga aaacgcgctt
    18361 caggtgaaag ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc
    18421 gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa
    18481 atagcgtcgc cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt
    18541 ttgttggaac catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc
    18601 gctcaagcgt gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta
    18661 aatccggctg aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag
    18721 gtagtttgtt ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc
    18781 actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc
    18841 ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat
    18901 ttcccggtaa acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc
    18961 agcaattcca gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc
    19021 tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg
    19081 ggcattcact ggtgattgag gatgacgtag ccgaagcgct ttatcaggag ctaaaacaga
    19141 aaaacctgat tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact
    19201 cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg
    19261 gcagttatgc ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac
    19321 aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta
    19381 cctttgctat cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg
    19441 tgattgccgg agcctcggca ctggttctca cctcttatct ggtgcgttgc aaaccgggtg
    19501 aacccatgcc ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg
    19561 tggtgctgac gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat
    19621 tcctcaaaga tcacgtctct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg
    19681 gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggtagat ctggtgctgt
    19741 gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta
    19801 aaacccagca tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc
    19861 gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt
    19921 acatgggcgg gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag
    19981 cgttgctgca tgacattacc gccaacagct accatcgtag caacgtacca aactccagca
    20041 aacataaatt cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc
    20101 gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc
    20161 gtgaagacag cctggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca
    20221 gataaggcgt tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc
    20281 gtcttatcat gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc
    20341 accacttcac ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt
    20401 tcacccatca ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa
    20461 tgggcgcggg cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca
    20521 cccgcttcat aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgcgcc
    20581 agttgcataa tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc
    20641 agctcatcaa cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc
    20701 aatttctccc ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa
    20761 atatccactg ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc
    20821 gtttcggtct tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc
    20881 gacaggatcg cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc
    20941 attcccagtc ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg
    21001 gttaatgccg tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat
    21061 ttaccaaaca gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt
    21121 aacggatcaa acaacatccc gacggagaca aaaaacagca ccgcaaacgc gtcgcgcaat
    21181 ggcagcgtat cgtgggcggc acggtgactc agttcagact cgttcagtac catcccggca
    21241 aagaacgcgc caagtgcgaa ggagacgtca aacaactcca ccgcaccaaa ggcaatccct
    21301 aacgccagcg ccagcaccga cagggtaaat agctcgcgag aaccggttgc cgcgctgcgt
    21361 gccataatcc acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc
    21421 actttgccga tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt
    21481 tccatcattc ctgccactgc gggcagcaac accagcgtca gaaccattac caggtcttcc
    21541 acaatcaacc aaccgatggc gatttgccca cgctgactgt caattaattg ccgttcttca
    21601 agtgcgcgca gtaacaccac ggtactggcg gtggaaagac ataaaccgaa cacgataccg
    21661 gtcattaacg accagcccag cacggcagag agcgccatac ccagcagcgt cgccacggct
    21721 atctgggcga tcgcaccggg aatggcgatg gcctttaccg ccatcaaatc cttcagcgaa
    21781 aagtgcaaac cgacgccaaa catcaacaga atgacgccca gttcagccag ttccggcgca
    21841 agcttggtat cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga
    21901 tatcccacca gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc
    21961 acaaggccgc caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt
    22021 cctggtggtt attgtccatt tttggccggg aaaaccaaaa ttacaggtaa tagtttatga
    22081 caatttcatt gatgatgttc atgaataatt attgaatttt gcagaaaaat ggaattagct
    22141 gcaaaaaaag cacggatcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga
    22201 ctgtaaaacg atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg
    22261 ttatcaggca ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag
    22321 actaactcga tgct
//
LOCUS       DAZSBF010000053        21641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZSBF010000053 DAZSBF010000000
VERSION     DAZSBF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.054"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="ACHXWD_001582"
     CDS             complement(<1..1125)
                     /gene="fsr"
                     /locus_tag="ACHXWD_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR7620525.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPRSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1343..2995
                     /gene="ushA"
                     /locus_tag="ACHXWD_001583"
     CDS             1343..2995
                     /gene="ushA"
                     /locus_tag="ACHXWD_001583"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR7620526.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="ACHXWD_001584"
     CDS             complement(3032..3511)
                     /gene="ybaK"
                     /locus_tag="ACHXWD_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR7620527.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3715..4509)
                     /locus_tag="ACHXWD_001585"
     CDS             complement(3715..4509)
                     /locus_tag="ACHXWD_001585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR7620528.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4647..4988
                     /locus_tag="ACHXWD_001586"
     CDS             4647..4988
                     /locus_tag="ACHXWD_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR7620529.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5203..7707)
                     /gene="copA"
                     /locus_tag="ACHXWD_001587"
     CDS             complement(5203..7707)
                     /gene="copA"
                     /locus_tag="ACHXWD_001587"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR7620530.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7969..8901
                     /gene="glsA"
                     /locus_tag="ACHXWD_001588"
     CDS             7969..8901
                     /gene="glsA"
                     /locus_tag="ACHXWD_001588"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR7620531.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8904..10196
                     /locus_tag="ACHXWD_001589"
     CDS             8904..10196
                     /locus_tag="ACHXWD_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR7620532.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10227..10324
                     /locus_tag="ACHXWD_001590"
                     /pseudo
     CDS             10227..10324
                     /locus_tag="ACHXWD_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10321..10728
                     /gene="cueR"
                     /locus_tag="ACHXWD_001591"
     CDS             10321..10728
                     /gene="cueR"
                     /locus_tag="ACHXWD_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR7620533.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10729..11187)
                     /locus_tag="ACHXWD_001592"
     CDS             complement(10729..11187)
                     /locus_tag="ACHXWD_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR7620534.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11184..12101)
                     /locus_tag="ACHXWD_001593"
     CDS             complement(11184..12101)
                     /locus_tag="ACHXWD_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR7620535.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12247..12924
                     /gene="fetA"
                     /locus_tag="ACHXWD_001594"
     CDS             12247..12924
                     /gene="fetA"
                     /locus_tag="ACHXWD_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR7620536.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12911..13690
                     /gene="fetB"
                     /locus_tag="ACHXWD_001595"
     CDS             12911..13690
                     /gene="fetB"
                     /locus_tag="ACHXWD_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR7620537.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="ACHXWD_001596"
     CDS             complement(13753..14607)
                     /gene="cnoX"
                     /locus_tag="ACHXWD_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR7620538.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="ACHXWD_001597"
     CDS             complement(14668..15477)
                     /gene="ybbO"
                     /locus_tag="ACHXWD_001597"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR7620539.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="ACHXWD_001598"
     CDS             complement(15467..16090)
                     /gene="tesA"
                     /locus_tag="ACHXWD_001598"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR7620540.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16061..16747
                     /gene="ybbA"
                     /locus_tag="ACHXWD_001599"
     CDS             16061..16747
                     /gene="ybbA"
                     /locus_tag="ACHXWD_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR7620541.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16744..19158
                     /gene="ybbP"
                     /locus_tag="ACHXWD_001600"
     CDS             16744..19158
                     /gene="ybbP"
                     /locus_tag="ACHXWD_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR7620542.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="ACHXWD_001601"
     CDS             complement(19392..20486)
                     /gene="mnmH"
                     /locus_tag="ACHXWD_001601"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR7620543.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20555..21481)
                     /gene="allS"
                     /locus_tag="ACHXWD_001602"
                     /pseudo
     CDS             complement(20555..21481)
                     /gene="allS"
                     /locus_tag="ACHXWD_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5798 g         5144 t
ORIGIN      
        1 atcggcgata agccccagaa ctgccgctcc cagacctccc atgccaaaag caaaaccgaa
       61 aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt agaccagaat
      121 ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc ccgtccagtg
      181 caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag agccccaaat
      241 cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac ctgccgcaac
      301 ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc cgaatttttg
      361 catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt tcgagaaaat
      421 gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg gattgattat
      481 cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac ggctaatttg
      541 cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc
      601 ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa agttgccgcc
      661 gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac gggctacgcg
      721 agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg ccagcagaac
      781 tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa agcacatgcc
      841 aattggcaac gaccgcggca tcggatattt atcggtccag tagccgacca ctggttgcag
      901 tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa atgtcagaga
      961 aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca tgtcgttcag
     1021 cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct tggtcgttga
     1081 cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa agtcactttt
     1141 tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta acatacctgt
     1201 atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg gatgcagatt
     1261 tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt caacacgttg
     1321 aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt
     1381 aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata aaacctacaa
     1441 aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg aatatggcga
     1501 atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg ttgcggctga
     1561 aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc ccgagtctga
     1621 cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg acgcgatggc
     1681 gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg aaaagtgggc
     1741 caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc gcctgtttaa
     1801 accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc tgacaaccga
     1861 tgacacagca aaaattggta acccggaata cttcactgat atcgaatttc gtaagcccgc
     1921 cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag acattattat
     1981 cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg caccgggcga
     2041 tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc
     2101 gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg tgccgggtac
     2161 gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg agtggggcaa
     2221 atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg ttaactacca
     2281 gctgattccg gtgaacctga agaagaaagt gaccagggaa gacgggaaaa gcgagcgcgt
     2341 gctgtacacc cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca
     2401 gaacaaaggc aaagcgcagt tggaagtgaa aataggcgaa accaatggtc gtctggaagg
     2461 cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc tggcagccca
     2521 aatggatcgc actggtgccg actttgcggt gatgagtgga ggaggaattc gtgattctat
     2581 cgaagcaggc gatatcagct ataaaaacgt actgaaagtg cagccattcg gcaatgtggt
     2641 ggtgtatgcc gacatgatcg gtaaagaggt gattgattac ctgaccgccg tcgcacaaat
     2701 gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg cgaaagacgg
     2761 caaactcaac gacctgaaaa tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat
     2821 ggcgacatta aacttcaatg ccaccggcgg tgatggctat ccgcgccttg ataacaaacc
     2881 gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata tccagaaaag
     2941 ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc agtaatccga
     3001 aagtgccgga tatttgcatc cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt
     3061 tggcatcgag gatcttcgcc agatcgcctg ccgccagttc gatatccagt ccgcgcttgc
     3121 cgccagaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc gtcggcagac
     3181 gttttttctg ccccaatggg ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg
     3241 ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact tttttaagat
     3301 ccagttgacc tgcgaccggc gtaacggcca ctgccagatg tttcatatca ccgttcactg
     3361 ccaccagtag cgttttgtag acttgatccg gattcaaacc taattttttg acgacttcat
     3421 cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt
     3481 ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac
     3541 gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct aagagtattg
     3601 gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg acggcataat
     3661 aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat ttttttagcg
     3721 caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg cgaccacata
     3781 tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct gatgcatcag
     3841 cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat tttgcggagg
     3901 tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat gccagtgggt
     3961 cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca tggtaatctg
     4021 gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg cctgcaataa
     4081 ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag
     4141 aaccatcgcg atttgccaca gcggttgagt agaaaagagc gacggagaaa tgcccatctc
     4201 ctggctaatg tgctgcagat tttggagttg ctcctcgtta atgcgctctt ccagcgcctc
     4261 gcaagtaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct cgacgatcag
     4321 cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca tatcgtggct
     4381 acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg tgatatcgat
     4441 ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta cccggtacaa
     4501 cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca
     4561 aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt gcatcgctat
     4621 tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg acgacacccg
     4681 gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat gagttagcag
     4741 agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat cgtaaactca
     4801 ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat tttaggctaa
     4861 acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc caggaagaat
     4921 tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg
     4981 tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc acgccgcatc
     5041 cggcatccga gcaccattgc ctgatgcgac gcttacccgt cttatcaggc ctacaaacct
     5101 gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg
     5161 ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct tcggtttaaa
     5221 ccgcagcaac cggttggcgt tactcactac ggtaatcgac gagagcgcca ttgccgctcc
     5281 ggcaactacc gggttaagca gtgttcccgt aaacggccac aaaataccgg cggcgaccgg
     5341 aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt tgcgcagcgt
     5401 tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca tcagggtaat
     5461 cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga catccgcctg
     5521 ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac gtccttcact
     5581 ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa tcacttcatc
     5641 aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg tcaacatcac
     5701 cagacgatat cccgttttat gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg
     5761 atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca caggcgttgc
     5821 tccttgcgat gcctgagcag taatctccgc ttcgatagct ttggtaccaa cctgttgctc
     5881 atttaacagc gcctgattgc ccagcaataa cgcatgacct tcagcttcac cgctcacgcc
     5941 cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc tgcatatcac ctgctttatc
     6001 gaggatcgcc cgtgccagcg gatggctgga accttgctcc agtgccgccg ccagacgcaa
     6061 tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg acaacctgcg gcttcccttc
     6121 ggtcagcgtc ccagttttat cgaacacgat ggtatcgagt gtactggcgc gttgcagcgc
     6181 gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg aaataatcga
     6241 catcggcgtc gccagcccca gcgcacacgg acaggcgata atcagtaccg tggtggcaat
     6301 caccagggta taaacaattt gcggtgccgg accaaagaag taccagattg ccgcactgac
     6361 cagcgcaatc accaccacca caggtacaaa tacggctgag attttatccg ccagctgacc
     6421 gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccgtg acagcgtggt
     6481 atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca ctgtcccggc
     6541 atggacgctt tcgccttcgc ctttttgctg tgggattggt tcgcccgtca gcatcgcttc
     6601 atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat cgccggtcgt
     6661 caggcgcagt aacatccctg gctgaacttc cgccagcggc acgctttttt caccatctgc
     6721 ggtgaccact cgcgccgtcg gtggggttaa atcgagtaat ttctccagcg ccttagaaga
     6781 acgctgacgt gcgcgcgctt ccagcatatg gccgagatta atcagaccga taatcatcgc
     6841 actggcttca tagtaaagat gccgcgcttc catcgggaac cactgcggcc acaggttgac
     6901 gctcatcgaa tagagccacg ccacacccgt tcccagcgcc accagcgtat ccatcgtcgc
     6961 tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgccgg cgaaaaccat
     7021 cactgccagg gttatcaggc cgataactaa ccacaagctg cggttgtcag cggtgaccat
     7081 catgttatcg ccgatcatcc cccagaccat taccgggata cccacagcca gtgcaacaat
     7141 tgcctgccag cggaagcgct tcatcgtagc tacggcggtt tcttgctggc gctcgcggcg
     7201 tttagcgtca tcttcaatcg cttccgcacc gtagcccgct ttttccaccg cctgcactaa
     7261 atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc
     7321 ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac agctggcgca
     7381 gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg ctgccggaag
     7441 cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca gcggtttagc
     7501 ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga tcagctgttc
     7561 tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgcct gctcaacatc
     7621 cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc
     7681 cagggtcagg tcgatagttt gtgacataaa acactccttt aagacagttt tgactggctg
     7741 tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac caggcgggta
     7801 aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca tttatttctt
     7861 tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga aaatcggcac
     7921 cggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat gttagatgca
     7981 aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact taacggcgga
     8041 caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc ggcagtggct
     8101 atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg ctttgcactg
     8161 gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg
     8221 gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt tatcgcctta
     8281 gagttgcatg gcggcaaacc gctgtcgcca ctggtaaatg ctggcgctat tgccaccacc
     8341 agcctgatta acgctgaaaa tgttgaacaa cgctggcagc gaattttaca tatccaacag
     8401 caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga acaaacaacc
     8461 aacttccata accgggccat tgcctggctg ctgtactccg ccggatatct ctattgtgat
     8521 gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa tactgttgaa
     8581 ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac gcataaacgc
     8641 gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga agggctgtat
     8701 ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc
     8761 ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc accgctggac
     8821 gaagaaggca acagtgttcg cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc
     8881 tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt aacaaacccc
     8941 tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg gggatcttcg
     9001 cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg
     9061 gcggtattgt ggcgatgttt tccggttatg cctatgcgcg tctgggggcg agctatccca
     9121 gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc ttttcgctag
     9181 cgctctcgtt gctgtacctg ttgacgctgg cggtgagcat cgccatggtt gcccgtgctt
     9241 ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac cttattttgc
     9301 tctacgcgtt ggggatcatt gcggtgatga cgctttttaa ctccttaagc aaccatgcgg
     9361 tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta
     9421 ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc cccagctccg
     9481 gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt ggcatgatgg
     9541 cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg tttctggtgg
     9601 cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt agcgatgtat
     9661 cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct tctccgctgc
     9721 tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg gcttcagcca
     9781 ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc gaacgcgaac
     9841 tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaac attattgtcg
     9901 tggtgttgat tatgctgatg acggcggcgc taaatttagg ctcactcgcc agtgttgcca
     9961 gcgccacctt tttgatttgc tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg
    10021 atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg
    10081 ttggctttat ctacagtctg tggtcccagg gtagccgtgc gttgatatgg attattggct
    10141 cactcttact cagccttatt gtggcaatgg tcatgaagcg caataaaacc gtataacatc
    10201 tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc actggcaaaa
    10261 aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg
    10321 atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat tcgcttctat
    10381 gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg cacctacacg
    10441 cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg ctttaacctg
    10501 gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag cgccgacgtc
    10561 aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga gctgcaatcc
    10621 atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag cgccgactgc
    10681 ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt aagacgagac
    10741 ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg tacctgcacc
    10801 gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat gaccgcgccc
    10861 gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt
    10921 taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt tccaccacag
    10981 ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc aacccagcgg
    11041 caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca acaaataacc
    11101 atttccgccc agcatctcgg ctgccagcag caaaccgccg agactgagcc agaaaatatg
    11161 tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct gtctttcacc
    11221 agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg catcatcact
    11281 actttgctgt tactggaggt accgatctgc tgtaacgctt cggtgtattt ctgcgctacg
    11341 aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat tttggtggcg
    11401 cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt
    11461 tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag gatttccgcc
    11521 tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc
    11581 gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg
    11641 actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg tgagttgatg
    11701 ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt acggatgtta
    11761 gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataagc cgcgcgcggc
    11821 gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt atctttcgag
    11881 ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt gcgaccaatg
    11941 cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt atagcgacca
    12001 aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc gccgacaatg
    12061 accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac ctcctgttgt
    12121 accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga ttactgaaag
    12181 agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca gaaaaaggga
    12241 acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata tctggcgggt
    12301 gatgcgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt taagttaatt
    12361 accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc attgatcagc
    12421 ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa gccagaaatc
    12481 taccgccaac aagtctctta ctgcgcccag acaccgacgc tgtttggcga tacggtatac
    12541 gataatctga tctttccctg gcagatccgt aaccggcagc ctgacccagc catttttctc
    12601 gattttctcg aacgcttcgc cttgccggac agcattttga cgaaaaatat cgccgagcta
    12661 tctggtggtg aaaaacaacg catctcactg attcgtaacc tgcaatttat gccgaaggtt
    12721 ttattgctgg atgaaatcac cagtgcgctg gatgaaagta ataaacataa cgttaatgag
    12781 atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac acacgataaa
    12841 gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg agaaatgcag
    12901 gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg cattaatgct
    12961 ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga aagatattct
    13021 ctggagcgtc ggacgagcga taattcagct gattattgtc ggctatgtgc tgaagtatat
    13081 tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct gctttaatgc
    13141 ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct catcgtttat
    13201 tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag ggtcgattga
    13261 atttatcccg atgcaggtga tccctatcgc cgggatgatt gccggtaacg ccatggtagc
    13321 ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc aacagattca
    13381 ggagaaactg agtcttggtg cgacgccgaa gcaggcttca gcgatattga ttcgcgacag
    13441 tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct tagtgagttt
    13501 accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg cgattaaata
    13561 tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca ttattgcctg
    13621 ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga cgcaattgaa
    13681 gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg
    13741 atcaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg acgccagtgc
    13801 atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc
    13861 ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt cattgcgccc
    13921 aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg gattctccgc
    13981 cacctgctgt tgcaactgtt gaatttccgg cgtatcagcc gcctgcttca gcagttcgat
    14041 ttgcgccacc atcccctggt agcgggtgtc ctgatcctgt aacggaatgg ttttcagcac
    14101 cgcttctgca tcttcagaac ggttcagcgc aatcagcgtt tctgccaaca gcaggccgat
    14161 ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg catcggtgaa
    14221 attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag
    14281 cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga agccatctac
    14341 cggttgcccg ttctggaaca gatacaccgt cggaatcgcg cgcagaccaa actgggcggc
    14401 aatcatctgc tccgcatcgc agtccagctt cgccagaata aattgcccgt tgtactgcgc
    14461 cgcaaggctt tccagaattg gggttaactg caaacagtgc tggctacgtt cagaccaaaa
    14521 atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt
    14581 aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata ttttctttga
    14641 catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca tcacgcgccc
    14701 cggcagcagg cgcttaagca ccattactgc ccaagtcacc agcgttaccg gatagcgcat
    14761 cttcggcttc tcgctaataa aagcatggcg tactttgtcc accaccgctt ccggtcccaa
    14821 cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg tctggttgac
    14881 gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt taattccgct
    14941 gtggcgtagc tccatgcgca gcgcgtctga ccatgcctcc agcgcatatt tactggccgc
    15001 gtaagcgcca cgacccggtg tggagattaa tcccatcacc gatgatgtca tcacaatacg
    15061 cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa
    15121 aaagttggcg gaaaactgct gttccatctg cgcgcggctg atggtggaaa gggggccata
    15181 catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca gggcgatcac
    15241 ttcgtcggct gcgcgatcaa cactttctgg cgaatccaga tcgatcaaca cgccggtaaa
    15301 tcccatgctg ttcatacgct caacatcatc cggtttccgg caacctgcca gcacatgaaa
    15361 accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac atccggtaat
    15421 taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat gagtcatgat
    15481 ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt
    15541 tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct tccataaaaa
    15601 agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg ctaaaggctt
    15661 cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat ggttcagcgt
    15721 tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa
    15781 aaccacgcaa accgtcattg ccgcccagtt caaccagcac ccaacgcggc tgatgctgtt
    15841 tcagcagagc cggaaggcgc gccagtcctt gttgcgaggt gtcgccgctg atgctggcat
    15901 taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag gccgcgctgg
    15961 cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg tccgctgcgg
    16021 cggcacggaa ggttaacagg gccaggaaca ggaagggcaa atgccagcgg aaaacattgt
    16081 tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct ccatcctcac
    16141 cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg agtcgggatc
    16201 gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca gtggcgaagt
    16261 gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc
    16321 gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta acgcgctgga
    16381 aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc
    16441 gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc cggcacagct
    16501 ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc gacctgatgt
    16561 gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata aaattgccga
    16621 cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga cccacgacct
    16681 gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt tgcaggagga
    16741 agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg
    16801 ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg
    16861 gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt
    16921 tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc
    16981 aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc
    17041 aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc ccctggcctg
    17101 aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct taacctgaaa
    17161 acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga agtgattcag
    17221 gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat gaatctggcg
    17281 gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg ttataaattc
    17341 ggcggcaacg agaaccagct cgacggctat gataaatggt tgttacctca gcttaaaccc
    17401 gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc
    17461 tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg
    17521 gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg
    17581 ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg
    17641 acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt gttgatggtg
    17701 ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc gtggctgtgg
    17761 gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata ccgcttgttg
    17821 ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg
    17881 aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg attaatgggt
    17941 ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt gctgtgcggt
    18001 gtgctgggct ggatgcagct gaatgtactt cgccgcatga cgctgaaatc gctacctctg
    18061 cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca gctttcggca
    18121 ttttcgctct cctttatgct gctggcgctg ttgctggtat tgcgtggcga tctgctcgac
    18181 cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa catcgccaca
    18241 gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc ggaatcattt
    18301 tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga aggtaatgaa
    18361 gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc cgatcataat
    18421 ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga agaggggctg
    18481 gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga tacccaggag
    18541 ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg gcctaatttc
    18601 tattttattt tcccggaagg ggcattagac gggcaaccgc agagctggct taccagtttc
    18661 cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc gaccattagc
    18721 ctgttagata ttggcgcgat tttaaaacag gtcggtcagg tgctggagca ggtaagtcgg
    18781 gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt gctggcacag
    18841 gtgcaggtgg gaatgcgtca gcgtcatcag gagctggtgg tgtggcgcac actcggtgcg
    18901 gggaaaaaac tgctgcgtac cacgttgtgg tgtgagttcg ccatgcttgg gtttgtttct
    18961 ggcctggtag ccgcaattgg tgcggaaacg gcgctggcag tgttgcaggc gaaagtgttt
    19021 gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag cggtgcactg
    19081 ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa ggcgctgttc
    19141 aggcagtttg cggggtgatg acaggtgtta gattgcctga tgcgctacgc ttatcaggcc
    19201 tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata aggcgtttac
    19261 gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa ctgtcatgtc
    19321 ggttttttat atctgtattc tggtgcaacc attctgttct cacgacaatc gccgtttcag
    19381 aataccgccc atcactgcgt cttaacccat tccgccactt ccgcccactc accgcgaaag
    19441 acaacttttt ccgctttttt ctcaagctga tagcgataca tcgggtcgta atattcttta
    19501 agtaacggca ccagccaggc cagatgaccg tcggtgctgt cggtggtgag ttgcgttgtc
    19561 agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag ccgacgctta
    19621 atagccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg ttcgtcgccg
    19681 tacgcgtggg taaaatcatg atgcatacgc aagaaatact cttcgttcag gcgctcaaga
    19741 cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg ctcgcgcagg
    19801 cattccggca ggtgattcga accgatcatc cggctttcgt cttccagcac ccacaggcgc
    19861 aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc aaaactcgcc
    19921 tggctaagtt gtggttgtaa ggtgcgacca aacgccgaac cgcgatgacg cgctaaccct
    19981 tccagatcaa caccgttcgg ctgttgctgc actaacagcg ttttaccgct gccggtacaa
    20041 ccgccaatca gcactatcgg tttttgtgcc agttcaatag tcgcctgaat cgcggcttgg
    20101 cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgctgc atacaaccag
    20161 ctttgcacaa tatgtgagcg ctgaccgcca cgggcgcagc agagaatacc ttgcggattt
    20221 tgcaggcacg ctgcccgcca ggcgtccatg cgctgctgac gaatttcacc cgccaccagt
    20281 ttatgcccca gcgccagcgc cgcgtctgag ccttgctgtt tataacaagt gccaacggcg
    20341 gcgcgttcat cgttattcat taacggcagg ttgatagcgg cgggcattgc gccttgctca
    20401 aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag ggcacgatag
    20461 tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag cttgcgcgct
    20521 attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtgggttg ccgaaaattt
    20581 ctaaaaatcc gctgatttcc ggcctgcgct gggtaaacaa ggtcacaata tcttctaccg
    20641 ctttgccgct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg cgcatcgttg
    20701 ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt gattttggca
    20761 aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata tcgggaacaa
    20821 taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtt cgggcgctgt
    20881 cttcaatatt gaccgccgga aagcgccgca actgcgcttc tgttagcggc tcttcaacgt
    20941 tcgccagcgg atgatccgcc gccatgacaa agcgccattg caccgatcct aagggatcaa
    21001 gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc gaaaaacctt
    21061 cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga aactgggtaa
    21121 aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg ttatagagca
    21181 ggttgttgat gacaatattc acctagcgtt ccacgccatc attcacctgt tgcagctcgc
    21241 tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga tgctcgccag
    21301 ccgctgtcaa cgtcacgctg cgagtcgtgc ggaaaaacag cgccactccg gtattctctt
    21361 ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt tctgccgctt
    21421 ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct ggatcgaaca
    21481 tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct ctgatttatc
    21541 agtattttta catgataacc ctgttcaatt tgtggactaa atctagtttt ggaaaaatat
    21601 tccaactttt gtattgatgt tgttctctta aggttttaga t
//
LOCUS       DAZSBF010000054        21483 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZSBF010000054 DAZSBF010000000
VERSION     DAZSBF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.055"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            288..4748
                     /gene="gltB"
                     /locus_tag="ACHXWD_001603"
     CDS             288..4748
                     /gene="gltB"
                     /locus_tag="ACHXWD_001603"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR7620544.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4761..6179
                     /gene="gltD"
                     /locus_tag="ACHXWD_001604"
     CDS             4761..6179
                     /gene="gltD"
                     /locus_tag="ACHXWD_001604"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR7620545.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6363..6767
                     /locus_tag="ACHXWD_001605"
                     /pseudo
     CDS             6363..6767
                     /locus_tag="ACHXWD_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            6859..7164
                     /locus_tag="ACHXWD_001606"
     CDS             6859..7164
                     /locus_tag="ACHXWD_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7620546.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(7240..7704)
                     /gene="nanQ"
                     /locus_tag="ACHXWD_001607"
     CDS             complement(7240..7704)
                     /gene="nanQ"
                     /locus_tag="ACHXWD_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR7620547.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7701..8576)
                     /gene="nanK"
                     /locus_tag="ACHXWD_001608"
     CDS             complement(7701..8576)
                     /gene="nanK"
                     /locus_tag="ACHXWD_001608"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR7620548.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8573..9262)
                     /locus_tag="ACHXWD_001609"
     CDS             complement(8573..9262)
                     /locus_tag="ACHXWD_001609"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR7620549.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9310..10800)
                     /gene="nanT"
                     /locus_tag="ACHXWD_001610"
     CDS             complement(9310..10800)
                     /gene="nanT"
                     /locus_tag="ACHXWD_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR7620550.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(10910..11803)
                     /gene="nanA"
                     /locus_tag="ACHXWD_001611"
     CDS             complement(10910..11803)
                     /gene="nanA"
                     /locus_tag="ACHXWD_001611"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR7620551.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(11925..12716)
                     /gene="nanR"
                     /locus_tag="ACHXWD_001612"
     CDS             complement(11925..12716)
                     /gene="nanR"
                     /locus_tag="ACHXWD_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR7620552.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            13096..14418
                     /gene="dcuC"
                     /locus_tag="ACHXWD_001613"
     CDS             13096..14418
                     /gene="dcuC"
                     /locus_tag="ACHXWD_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR7620553.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(14461..14958)
                     /gene="sspB"
                     /locus_tag="ACHXWD_001614"
     CDS             complement(14461..14958)
                     /gene="sspB"
                     /locus_tag="ACHXWD_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR7620554.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(14964..15602)
                     /gene="sspA"
                     /locus_tag="ACHXWD_001615"
     CDS             complement(14964..15602)
                     /gene="sspA"
                     /locus_tag="ACHXWD_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR7620555.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMCLGRS"
     gene            complement(15997..16389)
                     /gene="rpsI"
                     /locus_tag="ACHXWD_001616"
     CDS             complement(15997..16389)
                     /gene="rpsI"
                     /locus_tag="ACHXWD_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR7620556.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(16405..16833)
                     /gene="rplM"
                     /locus_tag="ACHXWD_001617"
     CDS             complement(16405..16833)
                     /gene="rplM"
                     /locus_tag="ACHXWD_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR7620557.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(17052..18179)
                     /gene="zapE"
                     /locus_tag="ACHXWD_001618"
     CDS             complement(17052..18179)
                     /gene="zapE"
                     /locus_tag="ACHXWD_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR7620558.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            18373..18771
                     /gene="zapG"
                     /locus_tag="ACHXWD_001619"
     CDS             18373..18771
                     /gene="zapG"
                     /locus_tag="ACHXWD_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR7620559.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            18925..20292
                     /gene="degQ"
                     /locus_tag="ACHXWD_001620"
     CDS             18925..20292
                     /gene="degQ"
                     /locus_tag="ACHXWD_001620"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR7620560.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            20382..21449
                     /gene="degS"
                     /locus_tag="ACHXWD_001621"
     CDS             20382..21449
                     /gene="degS"
                     /locus_tag="ACHXWD_001621"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR7620561.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT         5187 a         5698 c         5649 g         4949 t
ORIGIN      
        1 tgcgctaaag cacatttctg taccaataag cttgccattt gacctgtatc agctttcccg
       61 ataagttgga aatccgctgg aagctttctg gatgagcagc ctgctcatca tatttatgca
      121 gtaattgaga tcccctcttc accgtattaa ccgatgcgaa aaggacaaca agggggcgaa
      181 tgcgaggcgc gcgtatgaca cgcaaccccc gtcgccacgc tctttctgta cccgtgcgca
      241 gcggttcggt agtggggttc ccgcagagcc tgggggaggt tcacgatatg ttgtacgata
      301 aatcccttga gagggataac tgtggtttcg gcctgatcgc ccacatagaa ggcgaaccta
      361 gccacaaggt agtgcgtact gcaatacacg cactggcccg catgcagcac cgtggcgcga
      421 ttctcgccga tggtaaaacc ggtgacggtt gcggcttgct gttacaaaaa ccggatcgct
      481 tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc gaaaaactac gccgtcggga
      541 tgctattcct gaataaagat cctgaactcg ccgctgccgc acgccgcatc gttgaagaag
      601 aactgcaacg cgaaaccttg tcgattgtgg gctggcgtga tgtccccact aacgaaggcg
      661 tactgggtga aatcgccctc tcctctctgc cccgcattga gcaaattttt gtgaacgccc
      721 cggcaggctg gcgtccgcgc gatatggagc gccgtctgtt tatcgcccgc cgccgcattg
      781 aaaagcgtct cgaagccgac aaagacttct acgtctgtag cctatcgaat ctggtgaaca
      841 tctataaagg tctgtgtatg ccggcggatc tgccgcgctt ttatctggat cttgcggacc
      901 tgcgtctgga atcggccatt tgcctgttcc accagcgctt ctccactaac accgtaccgc
      961 gctggccgct ggcgcaaccg ttccgctatc tggcgcataa cggtgaaatc aacaccatca
     1021 ctggtaaccg ccaatgggcg cgtgcgcgta cctataaatt ccagacgccg cttatccctg
     1081 atctgcacga cgccgcaccg ttcgtcaacg aaaccggctc tgactccagt tcgatggata
     1141 acatgctgga actcctgctg gcaggcggga tggatatcat ccgcgccatg cgtctgttag
     1201 tgccacccgc ctggcagaac aacccggata tggacccgga actgcgtgcc ttctttgact
     1261 ttaactccat gcatatggag ccgtgggatg gcccggcggg catcgtgatg tccgacggtc
     1321 gttttgccgc ctgtaacctc gaccgtaacg gtctgcgtcc ggcgcgctac gtcatcacca
     1381 aagataagct catcacctgc gcctctgaag tcggtatctg ggattaccag cctgacgaag
     1441 tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt tatcgacacc cgcagtgggc
     1501 atattctgca ctcggcagaa accgatgacg atctgaaaag ccgccatcca tataaagagt
     1561 ggatggagaa aaacgtccgc cgactggtac cgtttgaaga tctgcccgat gaagaagtgg
     1621 gtagccgcga actggacgac gacacgcttg ccagctacca gaaacagttt aactacagcg
     1681 cggaagagct ggactccgta attcgcgtac tgggcgaaaa cggtcaggaa gcggtcggtt
     1741 cgatgggcga tgatacccca ttcgccgtgc tctccagtca gccgcgcatt atttacgact
     1801 acttccgcca gcagtttgcc caggtgacta acccgccaat cgacccgctg cgtgaagcgc
     1861 atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa cgtcttttgc gaagcagagg
     1921 gccaggcgca ccgtttaagc tttaaatcgc cgattctgct ctactccgat ttcaaacagc
     1981 tcacgacgat gaaagaggag cactaccgcg cagatacgct ggatatcacc tttgacgtca
     2041 ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga caaagccgaa aaaatggtac
     2101 gtagcggcac cgtgctgctg gtgctctccg accggaatat cgctaaagat cgcctgccgg
     2161 ttccagcccc gatggcggtt ggcgcgatcc agacccgtct ggtcgatcaa agcctgcgtt
     2221 gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga tccgcaccac ttcgccgtgt
     2281 tgctgggctt cggcgcgacg gctatttatc cataccttgc ctatgaaacg ctgggccgcc
     2341 tggtagacac ccatgcgatt gccaaagatt atcgtaccgt gatgctcaac taccgtaacg
     2401 gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg catctccacc atcgcctctt
     2461 accgctgctc gaaactgttt gaagcggtcg gtctacacga tgatgtagtg ggcctgtgct
     2521 tccagggggc ggtcagccgc attggtgggg caagctttga agacttccag caggatctgc
     2581 tgaatctgtc gaaacgtgcc tggctggcgc gtaagcccat cagccagggc ggcctgctga
     2641 aatacgtcca cggcggcgaa taccacgcct acaacccgga cgtggtgcgc acgctgcaac
     2701 aagcggtaca aagcggcgag tacagcgact atcaggaata cgcgaagctg gttaatgagc
     2761 gtccggcaac cacgctgcgc gatctgctgg caattacgcc gggtgaaaac gcggtcaaca
     2821 ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt cgataccgct gcgatgtcta
     2881 tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga agcgatgaac agcatcggcg
     2941 gtaactcaaa ctccggtgaa ggcggtgaag atccggcgcg ctacggcacc aacaaagtgt
     3001 cgcgcatcaa gcaggtggct tccggtcgct ttggggttac tccggcgtat ctggtcaatg
     3061 ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc gggcgaaggc ggtcagttgc
     3121 cgggtgataa agtcactcct tacatcgcca aactgcgcta ttcggtgccc ggagtgacgc
     3181 tgatctcccc gccgccgcac cacgatatct actctatcga ggacttagcg cagctcattt
     3241 tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt gaagctggtt tccgaaccgg
     3301 gcgtaggcac catcgcgact ggcgtggcaa aagcttatgc cgatttaatc accatcgcag
     3361 gctatgacgg tggcaccggc gcaagcccgc tgtcgtcagt gaaatacgca ggttgcccgt
     3421 gggagctggg gcttgttgaa acccagcagg cgctggttgc caatggtctg cgtcataaga
     3481 tccgtttgca ggtcgatggc ggcctgaaaa ccggtgttga tatcatcaaa gcggcgattc
     3541 tcggcgcaga aagcttcggc ttcggcactg gcccaatggt cgcgctcggc tgtaaatatc
     3601 tgcgtatttg ccacctgaac aactgcgcaa cgggtgtggc aactcaggat gacaaactgc
     3661 gtaagaatca ctatcacggc ctgccgttca aggtgacgaa ttactttgag tttatcgccc
     3721 gtgaaacccg cgagctgatg gcacagctgg gggtaacgcg gctggtggat ctgattggtc
     3781 gcaccgacct gctgaaagag ctggacggtt tcaccgccaa acagcagaaa ctggcgctgt
     3841 cgaagctgct ggagaccgcc gaaccgcatc caggtaaggc actctactgc accgaaaaca
     3901 acccgccgtt tgataacggc ctgctgaacg cgcagttgct gcaacaggcg aaaccgtttg
     3961 tcgatgaacg ccagagcaaa accttctggt tcgatatccg caataccgat cgctccgtgg
     4021 gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga tcaggggctg gcagccgatc
     4081 ctatcaaagc gtacttcaac ggcaccgcag gccagagttt cggtgtatgg aacgcgggcg
     4141 gcgtggaact gtacctgacc ggggatgcca acgactatgt cggtaaaggc atggcgggcg
     4201 gcttaatcgc cattcgtcct ccggttggtt ccgccttccg cagccatgaa gcaagtatta
     4261 tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct gtatgccgca ggccgcgcgg
     4321 gtgaacgttt cggcgtgcgg aactccggtg ctatcaccgt ggtagaaggc attggcgaca
     4381 acggctgtga atatatgacg ggcggtattg tctgtattct gggtaaaacc ggcgttaact
     4441 tcggtgcggg catgaccggc ggcttcgctt acgttctcga tgaaagcggt gatttccgca
     4501 aacgcgttaa cccggaactg gtcgaggtct taagcgttga cgatctggcg atccatgaag
     4561 agcatctgcg cggtcttatc accgagcatg ttcagcatac tggctctcag cgcggtgaag
     4621 agattctggc gaactggtca accttcgcca ctaaatttgc gctggttaaa ccgaagtcca
     4681 gtgatgtaaa agcactgctg ggtcatcgta gtcgtagcgc agcagagttg cgcgtgcagg
     4741 cgcagtaagg ggtagcaaca atgagtcaga atgtttatca atttatcgac ctgcagcgcg
     4801 ttgatccgcc aaagaaaccg ctgaagatcc gcaaaattga gtttgttgaa atttacgagc
     4861 cgttttccga aggccaggcc aaagcgcagg ctgaccgctg cctgtcgtgc ggcaacccat
     4921 actgcgagtg gaaatgtccg gtacacaact acatcccgaa ctggctgaag ctcgccaacg
     4981 aggggcgtat ttttgaagcg gcggaactgt cgcaccagac caacaccctg ccggaagttt
     5041 gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc ctgcactctg aacgatgagt
     5101 ttggcgcggt gaccatcggc aacattgagc gttatatcaa tgataaagcg ttcgaaatgg
     5161 gctggcgtcc ggatatgtcc ggcgtgaagc aaaccggtaa aaaagtggcg attatcggcg
     5221 caggcccggc aggtctggcg tgtgcggatg tcctgacgcg taacggcgta aaagccgttg
     5281 tcttcgaccg tcatccagaa attggcgggc tgctgacctt cggtattccg gccttcaagc
     5341 tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac cggcatgggt attgaattca
     5401 aactcaatac cgaagtgggc cgcgacgtgc agctggacga tctgctgagt gattacgatg
     5461 ccgtcttcct tggcgtcggg acttatcagt caatgcgcgg cgggctggaa aacgaagacg
     5521 ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa caccaaacag ttaatgggct
     5581 ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg caaacgcgtg gtggtccttg
     5641 gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt gcgccaggga gcgaagcacg
     5701 ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg ttcccgccgc gaagtgaaaa
     5761 acgcgcggga agaaggcgta gagttcaaat tcaacgtcca gccactgggg attgaagtga
     5821 acggtaacgg caaagtcagc ggcgtaaaaa tggtacgtac cgaaatgggc gaaccggatg
     5881 ccaaaggccg tcgccgcgcg gagatcgttg caggttccga acatatcgtt ccggcagatg
     5941 cagtgatcat ggcgtttggt ttccgtccac acaacatgga atggctggca aaacacagcg
     6001 tcgagctgga ttcacaaggc cgcatcatcg ccccggaagg cagcgacaac gccttccaga
     6061 ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg tggttccgat ctggtggtaa
     6121 ccgctattgc cgaaggtcgt aaggcggcag acggtattat gaactggctg gaagtttaag
     6181 ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc tttctttctg
     6241 gaaagcgcct cgcataatct gaagaaaacc aatgtaatac gctcatctgg attgttaccg
     6301 gtagatgccc ggcgtatagc tgattccatt attcaacgca gcgccatata aagggacaga
     6361 taatggaatc gctctcagag agaacctcaa caggctacca gcaaatccac gacggtatta
     6421 ttcacctggt cgatagcgcc cggacggaaa cggtacgtag cgttaacgcg ctgatgaccg
     6481 cgacatactg ggaaattggc cgacgaattg tcgaatttga acaaggtggc gaggcaaggg
     6541 ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga tttaagtcta aggtataagc
     6601 gtggtttctc tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt tttcaacatg
     6661 ttgaaattcg ccagacagtg tctggcgaat taacaccatt gggaattccg cagacaccat
     6721 ctgcagaatt cccatcggca aagatttggc agacgctgtc tgccaaattg agaggctgtt
     6781 tgatatttcc acagcttttg tattgcatct cctattttcg gtttgtatat tcatgcaaat
     6841 aaacgaagga aagttgccgt gatttacatt gaacagcttg aattaatcca taaaagtggt
     6901 gatgtgttgt atccagtaaa aataaccaga aaatcatcag gaaaaactgc cttccacctt
     6961 gttccattcg gcttaaataa gactcacgac cttctagagg tggaagatgc ttcagaagca
     7021 attcggctgg tgattgatga acgtcattcc attcgctgca gtacacttac agcaactatt
     7081 acgaataaaa agggcaagcg aattaagcgg acaggtatat acagtatcaa gggtgttaac
     7141 ataaaagaat acaacgtcag gtaatggcac agagcggtcc gtcagttcag gtttggctct
     7201 gtgccaaaga tgggtcaggt cacacctgat ctaataaatt taagccatta aatcagcctt
     7261 aaccttcacc accacctttt taatctcacc cggctcgcca acaacgcatc ccggcttatg
     7321 cggctctcct agcataaaca cggcgaacat tcccggtttc aggatgatgg cttgctcgtt
     7381 ttcaatggtg ctgcaaagct ggtaatcatc ctcatggtgg aactcttcac actgacgcgc
     7441 agtgcctgcc atgccaaaca gaatccgttc ttcaccgttt aataacagct ggatatcaat
     7501 gtattgctcg tgcagctccg cttttttctc aacgggcgat tgagtgttaa acgtcatgac
     7561 attcataaaa atattgtcgc cctgtaattc gtaacgaccc ggcgcttttt cttgcggtct
     7621 ggcagctaat gccagcgtta acgcgtcctg taacgcagga tgtaacccag cagacggtaa
     7681 tgaccgtact tcacccatca tcataatttt tctccctggg ccaacagcgc tgccccaagt
     7741 aaacctgcat catggcggta gtgcgccgcc agtaaatcaa catgaaatac cgctggctcc
     7801 tgcgccagat acgtttccac caacgccaga tacccttctg ccagaccaac gctgccaccg
     7861 accaccacgc actggcaatc agttgtggct ttaatatcag cgatcagcct tgccagcaca
     7921 tgtgcggagc ggtgaatcag ttgctgcgcc tgctcgtcgc cctgcccggc gcgtgtgaaa
     7981 atagttctcg catccgcgcc catgagttca ccttgcgctg ccgctgcaat accgcgacca
     8041 gaagcaatcg cttccacgca acctgtgcgt ccacagccgc agactgggcc gtgtggatcg
     8101 gcaagcgtat gcccgatatg ccccgccaga ccgccagggc cggtaagcag tttaccgccg
     8161 ctcactacac cgccgccgac gccagtggaa acggtgataa acaccatctc ggttacatcg
     8221 ccttccagcg cctgatactc tgcccatgct gcggcctgcg cgtcgttaat ggcaatggtc
     8281 ggcaaattgg taagttgttc cagcgttttg actaacggaa agtgtagcaa tccaccgaga
     8341 ttatgcggat tcagcgccag caaactgccg tcgcgaataa ttccggttga agcgatggca
     8401 acccgctgcg catgagcttg caacggagag actaatgcgg ataaggcatc acgcaaggct
     8461 tctggtgtct ggctggctgg cgtaggaagt tcacgacgat cgcggatctg cccgtcagcg
     8521 ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa tcgccagtgt ggtcatagca
     8581 ccgccttttt catcgctgtg ttgtaccact gacaaatgtg ttcaagacgc gtaatggcag
     8641 aaccgaccgt caccgcccac gcgccgtggc gcatcgcatc tgccgcctga gcaggcgtgt
     8701 tgtaacgccc ttcggcaatc acccgacatc cggcttcgct caacgttttc accagcgcca
     8761 gatccggctc ttctggcgtt tcaggcgtgg tatagccaga aagcgtagtg ccaataattt
     8821 cggctcccag cttttggcat gccaggccgt cttccggcgt tgagcagtcg gtcatcgcca
     8881 gtaaaccgtg atgatgtata cgtgccagca gcgtttcaac aggcaccgga cgcgggcggt
     8941 cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc cagcgcatcg acatcttcaa
     9001 tataggccgt gatgcgtacc ggagaatcct ccagatcgcg tttcacaatt ccaataatcg
     9061 gcacgctcac caccgcacgc gtggcttgca gatttgccac accttcgatg cgaatggcaa
     9121 ccgcgcccgc ctgctctgcc gctaatgcca tggctgcgac gatttcgggt ttatcgagcg
     9181 ggctgtcagg aatcggctgg caggagacaa tcaggccacc gttagcagcg attttttgat
     9241 ccagttgtgc aagtaacgac atacatcttc ccttagcgaa aggcccggta catagaccgg
     9301 gcaacaggat taacttttgg ttttgactaa atcgtttttg gcgctgccaa atggcacggc
     9361 accgctaaat ggtttgccgt cgatagcgtc atgagtacgc aatgcttccg ggcgcagcca
     9421 acgctgaacg cgggaaggca tatccagacc aatcagcagg atcaccacaa atgtcaggct
     9481 gaacgagagc gatgccagcg cagtacccag atccagacgt tgagcgatca acgcgccgat
     9541 gattggggcc agtgcgccac ccaatgcgcc aacgttgtag gtaaagccca ggcccgctgc
     9601 acgctggtcg gtatcgaaat aaccgccaat cagtttcggt aagatcccgg cgatcccttg
     9661 tccaagcatt tgctggaaga acagtaacag accgagcacc cagacgtttg cgccgccaat
     9721 cgcaaatacc ggaataatca gcagctgcga ggccagcagg ctacaaacgt acgctttgcg
     9781 ggttcccagc cagtcaccga ggaagcctcc tacgcagcat cccacggccg cgccaaagcc
     9841 actaaagaac agcacattgg cgacagtatg cgggttataa gccagatcgg ttttcagata
     9901 cgttggcagc agcgcctgaa tcggccatga gtagaggaaa gcaaacaaca cgaccaccat
     9961 cagcattacg cccgttggcc agcgtttgcc tgtactctgc accataaagc tgataaagat
    10021 tgcggcgcat aacagcccaa gaacagcgac gatcgcggca ttttgcaggt taccggcgaa
    10081 gcagaaccac agcgcagtag ccgccgccag tgtcattacg atattggcaa tgcgatgttc
    10141 accacggtag agaatatcca ccattgtgcg tactggtgct ttacctgcgt gtttctcttt
    10201 ccagtcttcc gcttccggga tgtttttacg cagccagaga gcaaagatga ttggcaaaat
    10261 gccgataaag aacagcgcac gccaacccca gaccggaacc accaggctat agacctgagc
    10321 ggcaacgacg gcccccacag agaagcctga aatcaaaaaa ccactggctt tgttacgcag
    10381 atgttttggc cagctttcaa tgacataggt ggcgctggaa ccgtattcac ccgccatccc
    10441 catgccgatg accagacgag cgataaacat ggtgatgtag cctggcgcaa agccgcaggc
    10501 cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt gccagacgac gcccgtagcg
    10561 gtcacccata gcgccgagca tcaggccgcc gaaccagcga gagataaagg ctgcggagat
    10621 cagacttgcc gcctgcaccg tcgtcagccc gaattcaccc tgtacttcgg tgagtaccag
    10681 ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc aaccaggcag cggaaaatgc
    10741 gcgccattgt gcacggttga gatggcgata ccacgggata ttctgggttg tagtactcat
    10801 tgtgagtctc ccgcggtggg cgatgcccac acgctttggt atgaaaattg tagggtacag
    10861 atgcgtttat ttcccctcac ccggtagggg cgagcgaggg gaaaacaact cacccgcgct
    10921 cttgcatcaa ctgctgggcc agcgccttca gttctggcag atatttttca tctaccggtc
    10981 caaacggttt gcggcacagc ggcacagaaa cgacatccat ataatgcagg acagttttca
    11041 ggccgcggaa tacgccagtt ttgatcagta aatcaatgac tttgttgcac tcagtttgca
    11101 gtttctgcgc ggtctggata tcgccttctt tcagcgcctt cacgatcccc tgatatcgcc
    11161 agcccatgat gttgtaagta ctgccgatac caccatcagc gcctgccagc aagccggaag
    11221 cgaagatttc gtcgtaaccg ttatagagca ccagatcagg atgttcacga cggatctgtt
    11281 ccatctgata gagatcgcca gaagtctgtt tcagcgcacc gacgccaggc aacgtaacca
    11341 gggtgttgat ctgatccagg gtcagtttta ccccactcag ggctggaatg ttgtacacaa
    11401 ccatcggcaa accatccgct gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa
    11461 agctgaaagg atagtagaac ggcgtgacgg cggagacggc atcgaagcca taacgtttag
    11521 ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt
    11581 taatcttacc tttcgcctct tcggcgacga tttccagtac ctgttcacgc tcggaaaggc
    11641 tttgtacaaa ggcctcgccg gtcgaaccac ccacgtataa accgtcgatg ccctgctgaa
    11701 tattgaactg aaccaggcga cgcagactcg ctttatccag tgcttgttgt tggtcaaaag
    11761 gagtcaggag tgcagccatt acgccacgta aattcgttgc cataaatacc tctgaagtga
    11821 tacttgtctg ataaacgata tacctttata cctgttatac cagatcaatt aagcaacacc
    11881 ccatacagaa agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg
    11941 gtggtctgac cgaaagcgtg ccaggtagca gagacgctgt tgagatgcga ttgcaacgca
    12001 cgatcggctt cgtcaggatc atgacggcgg atcgcatcaa cgatcgcaat atgctgttga
    12061 tagctaacgt tgttatgctc gtgcagagcc tgatcagcca cggttggacg tgcggcgatc
    12121 agccagtcga gcagggcaac gtggatcgcc atgaagattg ggttaccagg gatctccgcc
    12181 agcacgcggt ggaaatcaac gtctgaacga atgaatgccg cgttgttatc cagcgactga
    12241 ctgttgattt ccagtgcttt tgccagcaaa tcgatttgct catcggtggc atgttcagcc
    12301 gcatagcgca ccagactgga ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca
    12361 ccgggatgag aaaggaaatc tttcgccatg ccggaaaact caccgatgat agtgtccgca
    12421 gaaggacgcg agacgcgagc gcgttcgccg ttgtttattt gcaccagacc tttgcgtttt
    12481 aacgctgcca gcgcttcgcg cacagaagga cgcccgacgt taaagaacgc catcagttcg
    12541 cgttcagacg gtaattgttc accttcgcca aattcacgac ggcggatcat ctgttccagc
    12601 tcttcttcca ccatttcgga gagtttttta cgcgccagcg ggcggctacg caagttgcga
    12661 ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg cgttcataag gcccattctg
    12721 taaggtcagt gtgattaaca tcatcagtga catcctatca caggattgaa agtaggggaa
    12781 aatggcaggt ttttctcttt gtgcctcatc attaccataa ttaacggaat aattaactat
    12841 tgcgaaaaat taatgtaacg cagataaaca catcccgatt gaattattca taagactatt
    12901 cacaagcatt atgaatatta tgaatgtgtt cttacaaaat aatcataagc gcatattttt
    12961 taatgaaaaa tcacatcacc tacaattaaa aacacgacat ccgcaccata aatagacttg
    13021 caaaaaatat aacatcattg ttttcaatct gccgtttatg ggattgaccg ttttcttttg
    13081 acacggagtt caacaatgtt cggcataatt atatctgtca tcgtattaat tacgatgggc
    13141 tatttgatcc tgaaaaacta caaacctcag gtggtgctgg ctgccgcagg tgtcttcctg
    13201 atgatgtgcg gagtctggtt agggttcggt ggtgtactcg atcccgccaa aagcagcggc
    13261 tacttgatcg tcgacattta taatgaaatc ctgcgcatgc tgtccaaccg cattgccgga
    13321 ttggggctgt cgattatggc ggtgggcggt tatgcccgct acatggagcg cataggagcc
    13381 agtcgcgcga tggtgagctt gctaagccgc ccgttaaaac tcattcgctc gccgtatatt
    13441 attctgtcgg caacttacgt catcggccaa atcatggcgc agtttattac cagcgcctcc
    13501 ggtctgggta tgttgctgat ggtcacctta tttccgacgc tggtgagtct gggagtaagt
    13561 cgcctctctg cggtggcggt tatcgcaacc acgatgtcca ttgagtgggg gattctggaa
    13621 acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa ttgccacata cttcttccac
    13681 taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg cgatctccca ctttttcgtg
    13741 caacgcgctt ttgacaaaaa agataaaaat atcaatcacg aacaggcaga gcaaaaaact
    13801 ctcgataatg tcccgccgct ctattacgcc attttacctg tgatgccgtt aatcctgatg
    13861 ctcggctcgc tgttcctcgc ccacgtcggg ctgatgaagt cagaactgca tctggtggtg
    13921 gtgatgttac taagtttgac ggtgacgatg tttgttgagt tcttccgcaa gcataacttg
    13981 cgcgaaacaa tggacgatgt gcaggcgttt tttgacggca tgggtacgca gtttgccaac
    14041 gtggtaacgc tggtggtcgc gggtgaaata tttgcgaaag gcttaacgac gattggcact
    14101 gttgatgcgg ttatcagggg tgcggagcat tctggtctgg gcggtattgg cgtgatgatt
    14161 attatggcgc tagtcattgc catttgtgcc attgtgatgg gctctggcaa tgcgccgttt
    14221 atgtcatttg ccagtcttat tccgaatatc gcagccggac tacatgtacc agcggttgta
    14281 atgattatgc cgatgcattt tgccacgacg ctagcgcgcg cggtttcgcc gattactgcg
    14341 gtggtgaagc ggacagcgat ccccatggca gtcggtttcg tggtgaatat gattgccaca
    14401 atcacgctat tttattaagt cattaaaaag acaaaacagg ccgcctgggc ctgttttgta
    14461 ttacttcaca acacgtaatg ccggtcgacc accgcgtggt ggctgcggag gttcatcgtc
    14521 aggatgagtg tcatcatcgt gatctggctt gtcgccatca ataaccgaca taacggtttc
    14581 gttgtctgcc gatgcctctt catcattcat gatgctggta tcttcatcgt aggcagcttc
    14641 aggctcaaac atcgtgcctg cgccattttc acgggcgtag atagccagca cggcagccag
    14701 cggcacagaa acctgacgcg gaatgccacc aaagcgcgcg ttaaagcgca cctcatcatt
    14761 cgccagttcc agattgccga cagcacgcgg cgcaatgttg agtacgattt gcccgtcacg
    14821 cgcatattcc ataggaacct gcacgccagg gagcgtcaca tccaccacca ggtgcggcgt
    14881 gagctggtta tccagcaacc actcatagaa tgcacgcagc agatagggac gacgtggtgt
    14941 tagctgtgac aaatccatac agattaactc cggcccagac acatttcacg ttctgcttca
    15001 gttaaagaag caaggaaaga gtcacgctca aagacgcggg tcatatagcc tttcagctct
    15061 ttcgcacccg ggccgctgag ctcgataccc agttgcggca gacgccacag cagcggagca
    15121 agatagcaat cgaccaggct gaactcatcg ctcaggaagt acggcttctg accgaagacc
    15181 ggcgcaatcg ccagcagttc ttcgcgcagt tgcttacgtg cggcatctgc ttcagaagct
    15241 gaaccgttga tgatggtgtt catcagcgtg taccagtctt tttcgatgcg atgcatgtac
    15301 agacggcttt caccgcgagc taccgggtaa acaggcatca gtggcggatg cgggaaacgc
    15361 tcatccagat attccataat gatgcgagat tcccacaggg tcagctcacg atccaccagg
    15421 gtcggaacgc tctgattcgg gttgaggtca atcagatcct gaggcggatt gtccttttcc
    15481 acgtgttcga tctcgaaact tacacctttc tcagccagca caatgcggac ctgatggcta
    15541 tagatgtcag taggaccgga aaacagcgtc attaccgaac gtttgttggc agcgacagcc
    15601 atgaaaacct ccaggtatag tcagaatttt tactgctacc agccaccagg tggccagtca
    15661 gaagttgtgt tacccaataa ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag
    15721 caatacccga catttgggca gaaaattgga tgatagttta ccagattttg cgaccattgt
    15781 ggtgagtgga tgccggaaat ggggaaaaag agatgcgctt tagtctgaaa tagttgactt
    15841 agacccttat tggcgatgtg gtttttgttt tgcctgtctt tcagacggca gcaaaaagca
    15901 acttcccatt ttttacgctg attcagaatt tagccataaa aaaacccgcc gaagcgggtt
    15961 ttttcgtaaa ttgttttctg ccggagcaga agccaattaa cgtttggaga actgcggacg
    16021 acgacgtgct ttacgcagac cgactttctt acgttcaacc tgacgagcgt cacgagtaac
    16081 gaagccagct ttacgcagtt cagaacgcag ggactcgtcg tattccatca gagcgcgggt
    16141 gataccgtga cggatcgcac cagcctgacc agagatacca ccacctttaa cggtgatgta
    16201 caggtccagt ttctcaacca tgtcgaccag ttccagcggc tgacgaacta ccatgcgggc
    16261 agtttcacga ccgaagtact gttccagaga acgttggttg attacgattt taccgttgcc
    16321 cggtttgatg aaaacgcgag ctgcggaatt tttgcggcga ccagtgccgt agtattgatt
    16381 ttcagccatt gcctataatc ccgattagat gtcaagaact tgcggttgct gtgccgcgtg
    16441 gttgtgctcg ttacccgcgt aaactttcag tttacggaac atagcacgac ccagcgggcc
    16501 ttttggcaac atgcctttaa ccgcgatttc aatcacacgc tcaggacggc gagcaatcat
    16561 ctcttcaaag ctcacttgtt tgataccacc gatgtggccg gtgtggtgat agtacacttt
    16621 gtcagtacgc ttgttgccgg ttacagcaac tttgtcagcg ttcagaacga tgatgtaatc
    16681 accggtatct acgtgcggag tgtattccgc tttgtgctta ccgcgtaggc gacgagccag
    16741 ttcagtagcc agacggccca gagttttacc ggtcgcgtca acaacatacc agtcgcgttt
    16801 tacggtttct ggtttagctg taaaagtttt cattaaaagc ttacccaata aatagttaca
    16861 cgttggtgaa cacccaaacg tcttcaattg ttgaggttca cacgacaaag tccggcaaac
    16921 ctaccccttc gaatagccta tgccagcaca caaaaagttt tgggaaaaaa actttcttgt
    16981 aacgtggggt cgcaggatta tagagaagtc ggggtcaaag atcgacccct ttttgtgatt
    17041 tgtgacaggt tttaacccgc caaatgctcg cgcttcagat actcttcgct ttgcatctct
    17101 tgcagacgtg acaggcaacg ctggaactca aacttcagcc gatcgccctg ataaatttca
    17161 tacagcggca cttctgcact caccactaat ttgacatggc gctcgtaaaa ctcatccacc
    17221 agcgcaataa agcgccgcgc ttcgctctcc atcaaccgcg tcataactgg tacatcaaac
    17281 aacatgaccg tatgaaagag acgtgagagc gcaatatagt catgctgact gcgggcgtcg
    17341 acgcacagcg tagtaaaaga gaccgccagc gtctggttct cgacgcccat tgtcgctaat
    17401 ggccgatggt tgatttctaa cgtcggtgaa ttttctcgtt tcccccccgc cagcgccaac
    17461 catagtttat ccatttgcgc ccgggtttca tcgtggagtg gcgaaagcca cagatgcgcc
    17521 tgagtgagtg tacgcagtcg ataatcaaca ccagcgtcca cgttcattac atcacaatgc
    17581 tgtttaatgg catcgattgc aggcagaaaa cgcgcacgtt gcaagccatt tcgataaagt
    17641 tcgtccggcg gaatatttga cgtcgctacc agggtaatac cgcgggcgaa caaggctttc
    17701 atcagaccgc caagtagcat ggcatcggta atatcagaaa caaaaaattc gtcaaaacag
    17761 agcacgtcag tttcggcttt aaagcgatcg gcaataattt ccagcggatc ggtctgcccc
    17821 tgtaaggcag ttagctcttc atgcacccgc agcataaaac ggtgaaagtg caggcgctgt
    17881 ttccgctctc ccggcaggct ttgatagaaa aggtccatca gccaggtttt cccgcgtcct
    17941 acaccgcccc acatatataa gccacgcact ggcgtatgct ttgtgtcttc gcgtttaccc
    18001 cacagcttac cgacccgcgc cattagccca ctcgtcctgg gggctggtgg cgtgctattg
    18061 atgagttcct gataaataat ttccaggcgg ctgacggcct ctttttgaac gtcgtcgggt
    18121 tgatggctgc cttcattgag cgccttcagg tattgcgatg ttggggtaac gctttgcatg
    18181 atcttattgt tattccttga ataatcggtg cgccgttgtt cacggttgac gaaaaaaagg
    18241 ccgttctaca ctacgcgata tgcagtcggg attccacttc tgtggaatta acggttatag
    18301 tggcataatc agccgcaggc atggagcctg aagccaacac cctacggaaa caaaagacaa
    18361 cgggagatgt tcatgacctg ggaatatgcg ctaattgggt tagtcgtcgg catcattatt
    18421 ggtgctgtgg ccatgcgttt tggtaatcgt aaactacgcc agcaacaggc gttgcagtac
    18481 gaactggaaa agaataaagc tgaactggac gagtatcgcg aagagctggt tagccacttt
    18541 gcccgcagcg cggaattact ggataccatg gcgcacgact atcgccagct gtatcagcac
    18601 atggcgaaaa gttccagcag cctgctgccg gaactgtctg ctgaagcaaa cccgttccgt
    18661 aatcgtctgg cagagtctga agccagcaac gatcaggcac cggtgcagat gcctcgcgac
    18721 tattctgaag gcgcatccgg cctgctgcgt actggcgcga agcgcgacta atttattttt
    18781 cgggcgcagc cattgcgccc tcctcttctc tccctccccg actatcattt aatctggtgt
    18841 ctcattgtta gccgtctgaa aattcaataa catcaaactg ttttgaatct cttttcttat
    18901 cattcaggta cgagagcagg aataatgaaa aaacaacccc agctgttgag tgcattagcg
    18961 ttaagtgtcg ggttaactct ctcggcgtca tttcaggccg tcgcgtcgat tccaggccag
    19021 gttgccgatc aggcccctct ccccagtctg gctccaatgc tggaaaaagt gcttccggca
    19081 gtggtgagcg tacgggtgga aggaacggcc agtcagggac agaaaatccc ggaagaattc
    19141 aaaaagtttt ttggtgatga tttaccggag caacctgcac aacccttcga aggtttaggc
    19201 tccggtgtca tcatcaacgc cagtaaaggc tatgtgctga ccaacaacca tgtgattaat
    19261 caggcacaga aaatcagtat tcagctcaat gatgggcgcg agtttgatgc aaaactgatt
    19321 ggtagcgatg accagagcga tatcgccctg ttacaaattc aaaacccgag caaattaacg
    19381 caaatcgcta ttgccgactc cgataaattg cgcgtcggtg attttgccgt agcggtcggt
    19441 aacccatttg gccttgggca aaccgccacc tctggcattg tttccgcatt aggacgcagc
    19501 gggttgaatc ttgaaggtct ggaaaacttt atccagacag atgcttccat taaccgcggt
    19561 aactccggcg gtgcactgtt aaaccttaac ggtgagttaa ttggcatcaa cactgcaatc
    19621 cttgcgcctg gcggcgggag cgtcgggatt ggatttgcca tccccagtaa tatggcgcga
    19681 acactggcgc agcagcttat cgactttggt gaaatcaaac gcggtttgtt aggcatcaaa
    19741 ggcaccgaga tgagtgccga tatcgccaaa gccttcaacc ttgacgtgca gcgtggcgcg
    19801 tttgtcagcg aagtgttgcc aggttctggc tcggcgaaag cgggcgtcaa agcgggcgat
    19861 attattacca gcctcaacgg caaaccgctg aatagctttg ctgagttgcg ctctcgtatc
    19921 gcgaccaccg agccgggcac aaaagtgaaa cttggcctgc tgcgtaacgg caaaccactg
    19981 gaagtagaag tgacgctcga taccagcacc tcttcgtcgg ccagcgctga aatgatcgcg
    20041 ccagcgctgg aaggtgcaac gttgagcgat ggtcagctaa aagatggcgg caaaggtatt
    20101 aagatcgatg aagttgtcaa aggaagccca gctgctcagg ctggcttgca aaaagacgat
    20161 gtgatcattg gcgtcaaccg cgatcgggtg aactcgattg ctgaaatgcg taaagtgctg
    20221 gcggcaaaat cggccatcat cgccctgcaa attgtacgcg gcaatgaaag catctatctg
    20281 ctgatgcgtt aatgtcgtaa accgggcatc aggcttacgt gtgatgtccg gttaactcgt
    20341 ggtatgctgc tgccgttccc ttttttaatg acgcctccat catgtttgtg aagctcttac
    20401 gttccgttgc gattggatta attgtcggcg ctattctgct ggttgccatg ccttcgctgc
    20461 gcagccttaa cccgctttcc actccgcaat ttgacagtac cgatgagacg cctgccagct
    20521 ataatctggc ggttcgccgc gccgcgccag cggtggttaa cgtttacaac cgtggtttga
    20581 acaccaactc tcacaaccag cttgagatcc gcaccctggg atccggtgta atcatggatc
    20641 aacgcggtta tatcatcacc aataaacacg tcatcaacga cgccgatcag atcatcgtcg
    20701 ccttacagga tggtcgtgta tttgaagcat tgctggtggg atctgactct ctaaccgatc
    20761 tggcggtact taaaattaat gccactggcg gtttacctac cattccaatc aatgcacgtc
    20821 gcgtaccgca tattggcgac gtggtactgg cgatcggtaa cccgtacaac ctcgggcaga
    20881 ccattaccca ggggattatt agtgccacgg gtcgaattgg tctgaacccg accgggcggc
    20941 aaaacttcct gcaaaccgat gcttccatta accacggtaa ctctggcggc gcgctggtga
    21001 actcgctggg cgaactgatg ggcattaaca cgctgtcgtt tgataagagt aacgatggcg
    21061 aaacgccgga aggtatcggc tttgcgattc ctttccagtt agcaaccaaa attatggata
    21121 agctgatccg cgatggtcgc gtgatccgcg gctacattgg cattggcgga cgtgagatcg
    21181 caccgctgca cgcacagggc ggtggtatag atcaactgca agggatcgtg gttaatgaag
    21241 tgtcacctga cggcccggcg gcgaatgcgg gtattcaggt caacgatctg attatttcgg
    21301 tggataacaa accggccatc tctgctctgg agacgatgga tcaggtggcg gaaattcgcc
    21361 ctggttcggt gatcccggtt gtagtgatgc gtgatgataa gcagttaacg ctgcaggtca
    21421 ccattcagga atatccggca accaattaag tcgagtgctc aaaacaaaaa accggagtct
    21481 gtg
//
LOCUS       DAZSBF010000055        21003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZSBF010000055 DAZSBF010000000
VERSION     DAZSBF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.056"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHXWD_001622"
     CDS             complement(<1..170)
                     /locus_tag="ACHXWD_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR7620562.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHXWD_001623"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHXWD_001623"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR7620563.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHXWD_001624"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHXWD_001624"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR7620564.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHXWD_001625"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHXWD_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR7620565.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHXWD_001626"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHXWD_001626"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR7620566.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHXWD_001627"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHXWD_001627"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR7620567.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHXWD_001628"
     CDS             4288..5151
                     /locus_tag="ACHXWD_001628"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR7620568.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHXWD_001629"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHXWD_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR7620569.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHXWD_001630"
     CDS             6489..7106
                     /locus_tag="ACHXWD_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR7620570.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHXWD_001631"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHXWD_001631"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR7620571.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHXWD_001632"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHXWD_001632"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR7620572.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHXWD_001633"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHXWD_001633"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR7620573.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHXWD_001634"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHXWD_001634"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR7620574.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHXWD_001635"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHXWD_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR7620575.1"
                     /translation="MNPTRYARICEMLARWQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHXWD_001636"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHXWD_001636"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR7620576.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHXWD_001637"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHXWD_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR7620577.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="ACHXWD_001638"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="ACHXWD_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR7620578.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTTLKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="ACHXWD_001639"
     CDS             18072..19781
                     /locus_tag="ACHXWD_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR7620579.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHXWD_001640"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHXWD_001640"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR7620580.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5712 c         5385 g         4823 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 tctacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgatgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggtt
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaattt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggtggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggta tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccacg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAZSBF010000056        20711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZSBF010000056 DAZSBF010000000
VERSION     DAZSBF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.057"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHXWD_001641"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHXWD_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR7620581.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHXWD_001642"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHXWD_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR7620582.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHXWD_001643"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHXWD_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR7620583.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHXWD_001644"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHXWD_001644"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR7620584.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHXWD_001645"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHXWD_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR7620585.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHXWD_001646"
     CDS             complement(8214..9107)
                     /locus_tag="ACHXWD_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR7620586.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHXWD_001647"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHXWD_001647"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR7620587.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHXWD_001648"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHXWD_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR7620588.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHXWD_001649"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHXWD_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR7620589.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHXWD_001650"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHXWD_001650"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR7620590.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHXWD_001651"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHXWD_001651"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR7620591.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="ACHXWD_001652"
     CDS             complement(14275..16539)
                     /locus_tag="ACHXWD_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFR7620592.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHXWD_001653"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="ACHXWD_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR7620593.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHXWD_001654"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="ACHXWD_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR7620594.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHXWD_001655"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="ACHXWD_001655"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR7620595.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHXWD_001656"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="ACHXWD_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR7620596.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4971 a         5732 c         5044 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacacc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAZSBF010000057        20399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZSBF010000057 DAZSBF010000000
VERSION     DAZSBF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.058"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(130..1779)
                     /gene="actP"
                     /locus_tag="ACHXWD_001657"
     CDS             complement(130..1779)
                     /gene="actP"
                     /locus_tag="ACHXWD_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR7620597.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1776..2089)
                     /locus_tag="ACHXWD_001658"
                     /pseudo
     CDS             complement(1776..2089)
                     /locus_tag="ACHXWD_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2289..4247)
                     /gene="acs"
                     /locus_tag="ACHXWD_001659"
     CDS             complement(2289..4247)
                     /gene="acs"
                     /locus_tag="ACHXWD_001659"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR7620598.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4641..6077
                     /gene="nrfA"
                     /locus_tag="ACHXWD_001660"
     CDS             4641..6077
                     /gene="nrfA"
                     /locus_tag="ACHXWD_001660"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR7620599.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6122..6688
                     /gene="nrfB"
                     /locus_tag="ACHXWD_001661"
     CDS             6122..6688
                     /gene="nrfB"
                     /locus_tag="ACHXWD_001661"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR7620600.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6685..7356
                     /gene="nrfC"
                     /locus_tag="ACHXWD_001662"
     CDS             6685..7356
                     /gene="nrfC"
                     /locus_tag="ACHXWD_001662"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR7620601.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7353..8309
                     /gene="nrfD"
                     /locus_tag="ACHXWD_001663"
     CDS             7353..8309
                     /gene="nrfD"
                     /locus_tag="ACHXWD_001663"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR7620602.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8329..10047
                     /locus_tag="ACHXWD_001664"
     CDS             8329..10047
                     /locus_tag="ACHXWD_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR7620603.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSHSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10040..10423
                     /gene="nrfF"
                     /locus_tag="ACHXWD_001665"
     CDS             10040..10423
                     /gene="nrfF"
                     /locus_tag="ACHXWD_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR7620604.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10420..11016
                     /gene="nrfG"
                     /locus_tag="ACHXWD_001666"
     CDS             10420..11016
                     /gene="nrfG"
                     /locus_tag="ACHXWD_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR7620605.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11358..12671
                     /gene="gltP"
                     /locus_tag="ACHXWD_001667"
     CDS             11358..12671
                     /gene="gltP"
                     /locus_tag="ACHXWD_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR7620606.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12750..13439)
                     /gene="yjcO"
                     /locus_tag="ACHXWD_001668"
     CDS             complement(12750..13439)
                     /gene="yjcO"
                     /locus_tag="ACHXWD_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR7620607.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="ACHXWD_001669"
     CDS             complement(13533..15680)
                     /gene="fdhF"
                     /locus_tag="ACHXWD_001669"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15261..15263),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR7620608.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="ACHXWD_001670"
     CDS             complement(15878..17344)
                     /gene="mdtP"
                     /locus_tag="ACHXWD_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFR7620609.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="ACHXWD_001671"
     CDS             complement(17341..19392)
                     /gene="mdtO"
                     /locus_tag="ACHXWD_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR7620610.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="ACHXWD_001672"
     CDS             complement(19392..>20399)
                     /gene="mdtN"
                     /locus_tag="ACHXWD_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR7620611.1"
                     /translation="PRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYASADTIDVV
                     PEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQIMLTQRSVD
                     AQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTAQRAAEADL
                     NAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGRVISLKTSV
                     GQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSI
                     GYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEP
                     Q"
BASE COUNT         4555 a         5435 c         5601 g         4808 t
ORIGIN      
        1 atcattttct acgactgtca aaaatcgctc attttttaat gagtttattt gtttaatatt
       61 ttgggaaaag gtgatgcatg ctggagagga agagtattcc ccggtcaggc gaccggggaa
      121 ggggagagat taatgtgcgc ggccttgctc aacgccaaag ccggtctggg agcggataaa
      181 ctgcgcgcgg aacagttcac gctcgcgcgc cccttccgct gagttatcgg ttgccgagaa
      241 gaaccagatg ccgaggaatg ccacggtgat agagaacagc gccgggtatt catacgggaa
      301 gatggctttt tcgtgaccaa ggatctgtac ccaaatcgtc gggccgagga tcatcagtac
      361 tactgcggta atcagcccca gccagccacc catcatcgcg ccacgcgtgg tcagtttcga
      421 ccagtacata gaaagcagaa tgatcgggaa gttacagctc gccgcgatgg caaacgccag
      481 ccccaccata aaggcgatgt tctgattctc aaacagcacg ccgaggataa tcgcaatcac
      541 gccgaggatc agtacggtga tttttgatac ccgcagctct tcacgttcgg tcgcgccttt
      601 tttgaagacg ttagcgtaca agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc
      661 cgcaaccacc gcgaggatag tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt
      721 gccgcccact gcattcgcca ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc
      781 cgccgcgtct ttatattccg gattcgcacc aaccagcatg atcgcgccga agccgataat
      841 aaaggtcaga atatagaagt agcccataaa cccggtggcg tagaacacgc tcttacgtgc
      901 ttcgcgggca tcgctgactg taaagaagcg catcagaatg tgcggcaagc ccgccgtacc
      961 aaacatcagt cccagaccca gagagagtgc ggagatcgga tctttcacca gcccgcccgg
     1021 cttcatgatg tcgacacctt tcgggtgtac cgccatcgct tcactgaaca gattgttgaa
     1081 gctaaagccg acgtgtttca tcaccataaa ggccataaag ctggcaccga acagcaacag
     1141 cacggctttg ataatctgta cccaagtggt cgccagcatg ccgccgaaca ggacgtacat
     1201 catcatcagc acgccgacca gcaccaccgc aatgtgatag ttaaggccaa acagcagctc
     1261 gatcagttta cctgcgccca ccatctgggc gataaggtaa agcgccacca ccaccagaga
     1321 accacaggcc gaaagaatac gaatcggccc ttgtttcaga cggtaagagg ccacatcggc
     1381 aaaggtgtag cgccccaggt tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca
     1441 gcccaccagg aagcccagcg agtaaattaa gccgtcatag ccggaggtaa acaccagcgc
     1501 ggagatcccc aagaatgagg cggcggacat atagtccccg gcaatcgcca gcccgttctg
     1561 gaagccagtg atattgccgc ctgcggtgta gtagtcgcta cgagaacgta cgagttttga
     1621 tgcccagtag gtaatgccga gcgtaaacac gacgaaaatc aggaacataa taatcgcctg
     1681 ccagttcgtt ggctggcgct ctacggcccc gctaatagca tccgcggcgt tagctgcgaa
     1741 agggagtgtg gcggcaagcg ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg
     1801 acttcattat taagacggtc gaattcgccg ttcgcccgcc agatgtagat accggtgaga
     1861 acaaaggaga tcacaatcac tccaacacca attggaatac cgcgtgtgac gctggtgttc
     1921 ggattcagcg gcgtgccagc cagccgggcg cgaaggcgat cagtaaaata aagccgatat
     1981 aaactgccag cataataatc gacaggatgg tggcaaaccg ttgccgtttt tcgactaact
     2041 ccctgaaatg cgcattgtct tctatccgct gataaatagt gccattcatc acagattctc
     2101 cagaggtaat gtagggattg ttttaattcc cgctccctta tgggagaagg ttaacgctcg
     2161 ggtaaccctt gccgaatgta ggccggataa ggcgtttacg ccgcatccgg caatcaatgc
     2221 ctgatgcgac gctgtcgcgt cttatcaggc ctacaaaccg ttaccgactc gcatccggca
     2281 attgtgggtt acgatggcat cgcgatagcc tgcttctctt caagcagctt ctcgactacg
     2341 ccaggatcgg caagcgtcga ggtatcgccc aggttgctgg tatcgcccgc cgcaattttg
     2401 cgcagaatac ggcgcataat tttgccggag cgggttttag gcagggagtc ggtccagtgc
     2461 agcacgtctg gcgtcgccag cgggccaatc tctttacgca cccagttgcg gacttctgcg
     2521 tacagttctg gtgacggttc ctccccgtga ttaagcgtga cgtaggcgta gatcgcctga
     2581 cctttaatat tgtgcggaat acctactacg gcggcttcgg caatcttcgg atgcgccacc
     2641 agcgccgact caatctctgc cgtccccaga cggtgaccgg agacgttcag cacgtcgtcc
     2701 acacgcccgg tgatccagta atagccatct tcatcgcgac gcgcgccgtc gccgctgaaa
     2761 tacatatttt tgaaggtgga gaagtaggtc tgttcaaaac gttcgtgatc gccaaacagc
     2821 gtacgcgcct gacccggcca ggagtcggtg atcaccaggc tgccttcggt agcgccttcc
     2881 agcgggttac cttcgttatc gaccagcgcc ggttgcacgc cgaagaacgg acgtgttgcc
     2941 gaaccggctt tcagctcggt agcgccaggc agcggcgtga tcatgaaacc gccagtttcg
     3001 gtctgccacc aggtatcgac caccggacat ttctcgttgc cgattttttt ccagtaccac
     3061 tcccacgctt ccgggttaat tggctcgccc acggaaccga gaatgcgcag cgacgaacgg
     3121 tcggtaccct cgatcgcttt atcgccttcc gccatcagcg cgcggatcgc cgtgggtgcg
     3181 gtatagagaa tattgacctg atgcttgtcc accacctgcg ccatacgggc aggcgtcggc
     3241 cagttgggta cgccttcaaa catcagcgtg gtcgcaccgc aggccagcgg gccgtacagc
     3301 aagtaactgt gtccggtcac ccagcccaca tcggcggtgc accagtagat atcacccgga
     3361 tgataatcaa agacatattt aaaggtcagc gccgcgtaca ccagataacc gccggtagta
     3421 tgcagcacac cttttggctt accggtagaa ccggaggtgt agagaataaa cagcggatct
     3481 tcggcgttca tcttttccgc ctggtgctga tcgctcgctt gctcaaccag gtcgtgccac
     3541 cacaggtcgc gcccttcctg ccagtcaatt ttcccgccag tacgcttcag taccaccaca
     3601 tgctctacgc tggtgacgtt cgggtttttc agcgcgtcat caacgttttt cttcagcgga
     3661 atactgcgcc cggcacgcac accttcgtcg gaagtgatca ccagtcgtga gttggaatca
     3721 ataatgcgcc cggcaacggc ttccggcgag aagccgccga aaatcaccga atgcaccgcg
     3781 ccaatgcggg cgcaggccag catcgcaacc gcggcttccg gcaccatcgg catataaatc
     3841 gccaccacat cacctttttt aatgcccagc tcgagcaggg tattggcgaa gcggcagacg
     3901 tcgcggtgca gctctttata gctgatatgt ttgctctggc tggcgtcgtc gccttcccag
     3961 atgatggcgg tacgatcgcc gttttcttgc agatggcggt caaggcagtt tgccgccaga
     4021 ttcagcgtgc cgtcctcgta ccatttaatg gacacattac cgggggcaaa ggaggtgttt
     4081 ttcaccttct ggtaaggttt gatccagtca agaatttttc cctgttcgcc ccagaaggta
     4141 tcaggtacgt taatagattg ttgatacatc gcctcgtact gctgagggtt tatcaggcaa
     4201 cggtctgcga tgttggcagg aatggtgtgt ttgtgaattt ggctcatgct tttgttctcc
     4261 ttgtagggtg ttaataatat gtggcataag cgttaaatgt aggggtattg gcagttttgt
     4321 ttagtatttg ggcgacagat cacgcaaaag tagaattgtg caaataaacg gcagggtaat
     4381 ttttgaaggt cagcaacaaa agttgattaa ttctttgagg aacatgcagt tatgcattgc
     4441 tgttgaaaaa gaggaagata ctaactaact ctaaagtggt attttacatg cacttacaat
     4501 tgattaaaga caacattcac agtgtggtta tttgttacac ataggggcga gcaatgtcat
     4561 gacagtgtag gtgcggttac tgtcgtgaaa aacaataaaa accgccattg taacaatggc
     4621 gcaattcgga tgaagcccct atgacaagga taaaaataaa cgcacgccgt atcttcagct
     4681 tattgattcc ttttttcttt ttcacttctg ttcacgctga acaaacggca gctcccgcaa
     4741 aacctgtaac tgtggaagcg aagaatgaaa cctttgcccc gcagcatccc gatcaatatc
     4801 tctcctggaa agccacctcg gaacagtcag agcgtgttga cgccctggcg gaagatccac
     4861 ggctggtgat cctgtgggcg gggtatccct tctcgcgcga ttacaacaag ccgcgtggac
     4921 atgcctttgc tgtgaccgat gtgcgtgaaa ccctgcgtac cggtgcgccg aaaaacgctg
     4981 aagatggtcc gctaccgatg gcgtgctgga gttgtaaaag cccggatgtg gcgcgtctga
     5041 tccagaaaga cggcgaagat ggctacttcc acggtaagtg ggcgcgcggc ggcccggaaa
     5101 tcgtcaacaa cttaggttgt gccgactgcc ataacaccgc ctcaccagag ttcgccaaag
     5161 gcaaaccgga gttaaccctt tcccgtccgt atgcggctcg cgcgatggaa gccattggta
     5221 aaccttttga gaaagccgga cgtttcgacc agcaatcgat ggtttgcggt cagtgccatg
     5281 tggagtatta cttcgacggc aaaaacaaag cggttaaatt cccgtgggat gacggcatga
     5341 aagtcgaaaa tatggagcag tattacgaca aaattgcctt ctctgactgg actaactccc
     5401 tgtcgaaaac gccaatgctg aaagcgcagc acccggaata tgaaacctgg acagcgggca
     5461 ttcacggtaa aaacaacgtg acctgtatcg actgccatat gccaaaagtg cagaacgccg
     5521 aaggcaaact ctataccgac cataaaattg gtaatccgtt tgataacttc gcccagactt
     5581 gtgcgaactg ccatacccag gacaaagctg ccttgcaaaa agtggtcgcg gaacgtaagc
     5641 agtcgattaa cgacctgaaa atcaaggttg aagatcaact ggttcacgct cacttcgaag
     5701 cgaaagcagc gctggatgca ggcgcgacgg aagctgaaat gaagccaatt caggacgata
     5761 tccgtcatgc ccagtggcgc tgggatctgg cgatcgcttc ccacggcatt catatgcacg
     5821 caccggaaga aggtttacgg atgctcggta cggcgatgga taaagcggcg gatgcacgca
     5881 ccaaactggc gcgcctgctg gcgaccaaag gcatcaccca tgaaatccag atcccggata
     5941 tctcaaccaa agagaaagcc cagcaggcca ttggcctgaa catggaacaa atcaaggccg
     6001 agaagcagga cttcatcaaa acggtgatcc cgcagtggga agaacaggca cgtaaaaacg
     6061 gtctgttaag ccaataaccc cgttccgcct cgcaaggggc ggaaaacaca atggagtgaa
     6121 tatgagcgta ttacgttcgt tattaactgc cggggtgctg gcgtcgggcc tgttgtggag
     6181 cctgaacgga attaccgcta cccctgcggc gcaggcatct gatgatcgct acgaagttac
     6241 ccagcagcgt aacccggatg ccgcctgtct ggactgtcat aaaccagata ccgaaggtat
     6301 gcatggcaaa catgcttccg ttatcaaccc gaataacaaa ctgccggtca cctgcaccaa
     6361 ctgtcacggc cagccatcac cgcaacaccg cgaaggggtg aaagatgtga tgcgctttaa
     6421 cgagccgatg tacaaggttg gagagcagaa cagcgtctgt atgtcctgtc atctgccgga
     6481 acagttgcaa aaagcgttct ggccgcacga tgtccacgta accaaagtgg cgtgcgccag
     6541 ctgccattcc ctgcatccac agcaagatac gatgcagacg ttaagtgaca aaggacggat
     6601 taagatttgc gtcgattgcc acagcgatca gcgcaccaat ccgaacttta acccggcgtc
     6661 tgttccgttg cttaaggagc agccatgacc tggtctcgtc gccagtttct caccggcgtc
     6721 ggcgtgctgg cagccgtcag cggcaccgca gggcgcgtgg tggcgaaaac gttgaatatc
     6781 aatggggtgc gttacggcat ggtgcatgac gagtcgttat gcatcggctg taccgcctgt
     6841 atggatgctt gtcgggaagt gaacaaggtg ccggaaggcg tctcgcgcct gacgattatt
     6901 cgtagcgagc cgcagggcga atttcctgat gtgaaatatc gcttcttccg taagtcttgc
     6961 cagcactgcg atcatgcgcc gtgcgttgac gtctgcccga ccggtgcgtc ttttcgtgat
     7021 gccgctagcg ggattgtcga tgttaatcct gatctctgcg tcggttgtca gtactgcatc
     7081 gccgcctgtc cgtaccgcgt gcgctttatc catccggtca cgaagacggc ggacaaatgc
     7141 gatttctgcc gtaaaaccaa tttgcaggcc ggtaagctgc ccgcgtgtgt tgaggcttgc
     7201 ccgacgaagg cgctgacgtt tggcaatctg gacgatccta acagtgagat ttcgcaactg
     7261 ctgcgccaga agcctactta ccgctacaag ctggcgctgg gaaccaaacc gaagctgtac
     7321 cgcgtaccgt ttaaatacgg ggaggtgagc caatgacgca gacttccgca tttcattttg
     7381 aatcgctggt gtgggactgg ccgattgcca tctacctgtt tttgattggt atttctgccg
     7441 gtctggtgac gctggccgtg ctgttacgtc gcttctaccc gcaggcgggc ggtgcagaca
     7501 gtacgttgct gcgcaccacg ctgattgtcg ggccgggcgc ggtgatcctc ggtctgttga
     7561 tcctcgtctt ccacctgaca agaccgtgga ccttctggaa gctgatgttc cactacagtt
     7621 ttacctcggt gatgtcgatg ggggtgatgc tgtttcagct ctacatggtg gtgctggtgc
     7681 tgtggctggc gaaaatcttt gaacatgatt tgcttgccct gcaacaacgc tggttgccga
     7741 agctggggat cgtgcaaaag gttctgagcc tgctgacgcc cgttcatcgc ggactggaaa
     7801 cgctgatgct ggtgttggcg gtgctgctgg gggcttatac cggctttctg ctgtcggcgc
     7861 tgaaatcgta tccgttcctc aataacccga tcctgccggt gctgttcctc ttctccggca
     7921 tctcgtccgg tgcggcggtg gcgctgatcg ccatggcgat acgccaacgc agtaacccgc
     7981 attccacgga agcgcacttt gttcaccgga tggaaatccc cgtggtatgg ggtgaaatct
     8041 tcctgctggt ggcgtttttt gtcggtctgg cgctgggcga tgacggtaaa gttcgtgcgc
     8101 tggtggcggc attaggtggc ggtttctgga cgtggtggtt ctggcttggt gtcgccgggc
     8161 tggggctgat tgtgccaatg ttgctcaaac cgtgggtcaa tcgcagttcc ggcattcctg
     8221 ccgtgctggc ggcgtgtggg gccagtctgg tcggcgtgtt gatgctgcgc tttttcattc
     8281 tctacgccgg acagttgacg gtggcgtaag ccagaaaaga ggtggtttct ggacgtattc
     8341 cttcctgaag tcggttttct ggcgttgttg ttaagtctcg gggtcaacgt gttgaccccg
     8401 ttgacggcct ttgcgggagt gcggttgcgc tggcctgcca tgatgcgact cacttgcatc
     8461 ggcattctgg cgcagttcgc gctcctgctg ctcgcctttg gcgtactgac gtattgtttt
     8521 ctcatcagcg atttctcggt catttatgtc gcgcaacata gccatagcct gctgtcgtgg
     8581 gaattcaagc tggcagcggt gtggggcggc catgaaggtt cgctgctgct ttgggtgctg
     8641 ctgctttccg cctggagcgc gctgtttgcc tggcattatc ggcagcaaac cgatccgcta
     8701 tttccgctga cgttagccgt tttatctctc atgctcgccg cactgctact gtttgtggtg
     8761 ctgtggtccg atcccttcgt gcggatattt ccaccagcaa tcgaaggccg cgatctcaat
     8821 ccgatgctgc aacatcccgg tcttatcttt catccaccgc tgctttacct tggctatggc
     8881 ggtttgatgg tagcggcgag cgtggcgctg gcgagtctac tgcgcggcga gtttgatgcg
     8941 gccagcgccc gaatttgctg gcgctgggcg ttacctggct ggagtgcatt aacggcgggg
     9001 atcatcctcg gttcctggtg ggcctactgc gaactcggct ggggcggctg gtggttctgg
     9061 gatccggtgg aaaatgcctc tttattaccc tggctttctg ccactgcgct gctgcacagt
     9121 ttatccctga cacgccagcg ggggattttt tgccactggt cgctgttgct ggcgatagtt
     9181 actctgatgc tgtcgctgct gggcacctta attgtccgtt ctggcattct ggtttcggtt
     9241 catgcgttcg cactggataa cgtccgcgcc gtgccgttgt tcagcctgtt tgcactgatt
     9301 agccttgcgt ctttggctct gtatggctgg cgagcgcggg acggtggccc ggcggtgcgt
     9361 ttttcggggt tatcgcggga aatgttaatc ctcgctacgc tgttgctgtt ttgcgcagtg
     9421 ctactgatcg tgctggtggg aacgctttat ccgatgattt acggtctgct gggctgggga
     9481 cgcctctccg ttggcgcgcc gtattttaac cgcgcgacgt taccgtttgg tctgttgatg
     9541 ctggtggtga ttgtgctggc gacgtttgtc tctggcaaac gcgcgcagct tccggcgctg
     9601 gtcgctcatg cgggcgtgct gttatttgcc gcagggatcg tggtttccag cgtcagccgt
     9661 caggagatca gcctgaattt acagccgggt cagcaggtga cgctggcagg atacaccttc
     9721 cgttttgagc gcctcgatct gcaagccaaa ggcaattaca ccagcgaaaa agcgatagtg
     9781 gcgctgtttg accatcagca acgcattggt gaattaacgc cggagcggcg tttttatgaa
     9841 gcacgccgtc agcaaatgat ggaaccgtca attcgctgga acggcatcca tgactggtat
     9901 gcggtcatgg gtgaaaaaac cggagcggat cgttacgctt ttcgtttgta tgtacaaagc
     9961 ggtgtgcgct ggatctgggg tggaggattg ttgatgattg cgggcgcatt gttaagcgga
    10021 tggcggggga agaagcgcga tgaataaagg gcttctcacg ctactgctgt tatttacctg
    10081 ttttgcgcgc gctcaggtcg tagacacctg gcaattcgcc aatccgcaac aacagcaaca
    10141 ggcgttaaat attgccagcc agttacgttg tccgcagtgc cagaatcaaa acttactcga
    10201 atccaatgcg ccggtggcgg tcagtatgcg ccatcaggtt tatagcatgg tgtcggaggg
    10261 gaagaacgaa gtcgaaatca ttggctggat gaccgaacgc tacggagatt ttgttcgcta
    10321 taacccaccg ttaacgggtc agacgctggt gttgtgggcg ctgccagtgg tgttgttatt
    10381 gctgatggca ctgatcctct ggcgagtgag ggctaagcga tgaaacagcc tcaaataccg
    10441 gtgaaaatgc tgacaacact cacgatattg atggtattcc tctgtgtcgg cagttatctg
    10501 ttaagcccaa aatggcaggc ggtacgtgcg gagtatcagc gtcagcgcga tccgctacat
    10561 cagtttgcca gccagcaaac cccggaagcg cagcttcagg cattgcagga taaaatccgc
    10621 gctaatccac aaaacagcga acaatgggcg ttactgggcg agtactatct gtggcaaaac
    10681 gattacagca attcgctgct ggcgtatcgt caggcgttgc aactacgtgg tgagaacgct
    10741 gaactgtatg cggcgctggc gacggtgctt tattaccagg ccagccagca tatgaccgcc
    10801 cagacttgcg caatgatcga caaagccctc gcgctggact ctaacgaaat caccgctctg
    10861 atgctgctgg cttccgatgc gtttatgcag gcgaattacg cgcaagccat cgaactatgg
    10921 caaaaagtga tggatctcaa ctcaccccgg atcaaccgga cacagctggt cgagtcgatt
    10981 aatatggcaa aactgttgca gcggagatcg gattaatcat ctggtgatga agattatgtc
    11041 attgagctgc ataaaaaata atcgaatgaa catatgccaa aaataatcac taatcagtat
    11101 tattgcagat taaacaaata aaaatctttc cataacaaat ggttattcat taatcctgcc
    11161 ataaactcgc gttattatgc attaatgcag cgaaaagctc tgttgttaaa gggttgcgca
    11221 acataccgcg caaatgatac tgatcataag cgttaaaaaa atctacaaac caacgcaaca
    11281 caattcatgc cctggcagta tgtcacgttc tcgcgtttct gaacggggaa cggcgctcca
    11341 ttgaggaagt tattctgatg aaaaatataa aattcagcct ggcctggcag attctgtttg
    11401 ctatggtgct gggcattctc ctgggaagct acctgcacta ccatagcgac agccgcgact
    11461 ggctggtcgt caatttgctc tctccggcgg gtgatatctt catccatctg atcaaaatga
    11521 ttgttgtgcc gattgtgatc tccacgctgg tggtgggtat cgcgggtgtt ggtgatgcca
    11581 aacagctcgg gcgtattggc gcgaaaacca ttatttactt cgaggtgatc accaccgtcg
    11641 ccatcatttt ggggatcact ctggcgaacg tcttccagcc cggtgccggg gtggatatgt
    11701 cgcagctggc gaccgtcgat atctcgaaat atcagagcac tacggaagcg gtacaaagca
    11761 gttcccacgg cattatgggc acgattttgt cgctggtgcc gacgaacatt gtggcgtcga
    11821 tggcgaaagg cgaaatgctg ccgatcatct ttttctcggt gctgtttggt ctggggcttt
    11881 cttccctgcc cgcgacgcat cgtgaaccgc tggtgaccgt gttccgctcc atctctgaaa
    11941 ccatgtttaa agtgactcac atggtgatgc gttatgcacc ggtgggtgtg tttgcgctga
    12001 ttgcggtgac ggtggctaac tttggtttct cttctctgtg gccactggcg aaactggtgc
    12061 tgctggtgca tttcgccatt ctgttcttcg cgctggtagt gctgggaatt gtggcgcgcc
    12121 tgtgcgggtt aagcgtctgg atcctgattc gtattctgaa agatgagctg attctggcgt
    12181 actccactgc cagctctgaa agcgtactgc cgcgaattat tgagaagatg gaagcctacg
    12241 gagcaccggc gtcgatcacc agcttcgtgg tgccgaccgg ttactctttt aaccttgatg
    12301 gttcgacgct gtatcaaagt attgccgcta ttttcatcgc tcagctgtat ggcattgacc
    12361 tgtccatctg gcaggaaatc attctggtgc tgacgctgat ggtgacctcg aaagggattg
    12421 ctggcgtgcc gggcgtgtcg tttgtggtgc tgctggcaac gctgggtagc gtaggtattc
    12481 cgctggaagg tctggcgttt attgctggtg ttgaccgtat cctcgacatg gcgcgtactg
    12541 cgctgaacgt ggtgggtaat gcgctggcgg tgctggtgat tgccaagtgg gaacacaaat
    12601 ttgaccgcaa aaaggcgctg gcttatgagc gtgaagtgct gggcaaattt gataaaactg
    12661 cggatcaata attgaagatt gccggggata tccacccggc aatgtgtgaa tgcctgatgc
    12721 gacgcttgcc gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca
    12781 cacccggtat caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg
    12841 ttcttctcga taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa
    12901 taaccggttc gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg
    12961 gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca
    13021 gagtcgttct ccagatcttc cgaggcgttt tctaacagcg aaatagcctt tggataatcc
    13081 ggtttacccg cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg
    13141 cccgcttgcg ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga
    13201 ttggtgattt tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct
    13261 ttctgtgccc aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga
    13321 aaatattgtg cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg
    13381 gagtcgttgg cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt
    13441 ttgataatac gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca
    13501 gcctccgaaa ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga
    13561 gttttcaact tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc
    13621 ggctcgacgc gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt
    13681 tcggtaacca gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct
    13741 ttgttcggac gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc
    13801 caaaccaatg cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg
    13861 tagccaggtt catcagccag cgccgccagt gccgcacagt taccggtcat cgaacggcaa
    13921 gagtagtgac caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg
    13981 tcgattggcg ctacccagtc gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac
    14041 ttctctttaa acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac
    14101 tgaatgaagc ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga
    14161 tgacgcaact catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg
    14221 gtggcgattt cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct
    14281 ttgaagaaac gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac
    14341 gacgttgacg gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga
    14401 acgataacca gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt
    14461 tgcagcggat cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc
    14521 ggcagctcgc tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg
    14581 gcgaatttct cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc
    14641 agcgcgccca tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc
    14701 gcatgcggct taccgaggtt accagtcagc atcgcgaggc tggtcagaga acgcacggtt
    14761 tccacgccct ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc
    14821 tgggcataca tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca
    14881 accgactccg gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg
    14941 gaagcgacga acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc
    15001 aacagcgcga tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt
    15061 tcaattttgc gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg
    15121 tgattcgcca cgattgggtg ggaatcagcc gggttgtacc cgaaaacgaa cactaaatcg
    15181 gtattatcaa tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga
    15241 cctgcaaccg atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata
    15301 acggcgcgcg caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag
    15361 ccggtcgtct ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggta
    15421 acgtaattca gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg
    15481 atcatggggg ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca
    15541 taataaccct tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg
    15601 acgattttgc cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa
    15661 accgtgacga cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct
    15721 cgactacagt atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg
    15781 gcactattta ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa
    15841 cgaaataccg cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag
    15901 ccgcctgata cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc
    15961 tgtccagcat cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct
    16021 gtaagcggct ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg
    16081 ttgcttccac gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc
    16141 cgttgacggc aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca
    16201 tgttgctggc ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg
    16261 gcaatttcag acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca
    16321 ggtggatggc atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc
    16381 gcgcggaatc cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg
    16441 ggcgtctggc gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta
    16501 atgccaccgg tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag
    16561 attctcgcgt ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa
    16621 tctgtgcccg cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct
    16681 ggtgcgcttt caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct
    16741 gatagctggc ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta
    16801 ctgctgcggt ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc
    16861 gatgcacacc ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat
    16921 agtacggccc gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt
    16981 tcgccgagac gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg
    17041 catctaacaa atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg
    17101 tgtgtgaacc acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt
    17161 tccaccactg cgcctgcggc cagccggagc tggcaagatg aatatcatcg gccagtttga
    17221 tttgttccgg tttgagctgt tgatgaggcg cagaatcctt gcgtaccaag gcacagccgg
    17281 aaatcagcgt cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga
    17341 tcattgcgtg gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc
    17401 tggcaagcgg gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat
    17461 ggcctgcgag gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc
    17521 cagaccggca gcgtattttt ccagggcgtc ggcaaactga cctgctctgt ccggctgtag
    17581 tgcgttatcc agcgcctgcg ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg
    17641 gccctgacga ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag
    17701 ttgacgctcc agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag
    17761 accgatacga atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg
    17821 cattacttta cttaacatac ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc
    17881 actttcaggc cagacaaagg tgtaaatcac cgcggacacc acggtaccaa tgatgatacc
    17941 cagggcgcga tcgcgaattt ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt
    18001 ggcgagcgcg aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt
    18061 ggcaatccat gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt
    18121 gtccagccag ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc
    18181 cccgccaaaa cgcagcacca tcttctggta cgacgaaccg acatttggat tagagacgat
    18241 cacgcaggtc agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta
    18301 acagatcaaa caggcgagca atgtttttac cgcgtagcgt atatagtctg gattggtaaa
    18361 agcatcggcg accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat
    18421 ctggcccagt tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccac
    18481 cgcttcactt tcactgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg
    18541 ctgcagcttg ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc
    18601 agcaaaagag gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac
    18661 gcagctttgc caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac
    18721 attcagtttt tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag
    18781 gctgtctgtc aggtgactaa tggcatcatc aagccgatca ttaagcgcct gatgcatttg
    18841 cgaaatggca cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata
    18901 gaggccaaca acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat
    18961 ggcggggaag gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag
    19021 ccgatgggtg cgcatcaaaa acatgcggcc catcaggatc ggcccggcga tgatcaatcg
    19081 gatcaacggt tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac
    19141 cgtggcaacc acaaacaaga tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc
    19201 gtaaaacagc actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag
    19261 aatcaccagc aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg
    19321 ctcgcttaac tcttcatgaa agaacgccag cagcctgacc atcggtaatg gcagggagtt
    19381 gagcgcgctc attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt
    19441 tccgggtcag gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag
    19501 ttaatagagc gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc
    19561 acgccgtagc caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg
    19621 cgaatcgttg cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagttggcg
    19681 atcacgtacc agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat
    19741 tgcccgacgg aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc
    19801 atttccagat gcagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc
    19861 agcgcatcga cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta
    19921 agatcggctt ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca
    19981 aaaccctctt tcagtaatgg ctcggtacgg cgtaatgtat ctgtggcctg tttcgcggcg
    20041 gcacgggctt tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg
    20101 ctacgctggg tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc
    20161 agattggctt cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc
    20221 tggttgtcgg tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg
    20281 gtatctgctg acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg
    20341 aaaacaaggg caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagg
//
LOCUS       DAZSBF010000058        20223 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZSBF010000058 DAZSBF010000000
VERSION     DAZSBF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20223
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.059"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..880
                     /gene="yieE"
                     /locus_tag="ACHXWD_001673"
     CDS             <1..880
                     /gene="yieE"
                     /locus_tag="ACHXWD_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR7620612.1"
                     /translation="HITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQL
                     GVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYI
                     LPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLED
                     ELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELG
                     NSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSN
                     ALRRFLDYCDPANVPR"
     gene            1085..2554
                     /gene="lysP"
                     /locus_tag="ACHXWD_001674"
     CDS             1085..2554
                     /gene="lysP"
                     /locus_tag="ACHXWD_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR7620613.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2846..4837
                     /gene="cirA"
                     /locus_tag="ACHXWD_001675"
     CDS             2846..4837
                     /gene="cirA"
                     /locus_tag="ACHXWD_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR7620614.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4869..5705)
                     /gene="yeiG"
                     /locus_tag="ACHXWD_001676"
     CDS             complement(4869..5705)
                     /gene="yeiG"
                     /locus_tag="ACHXWD_001676"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR7620615.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5963..6631
                     /gene="folE"
                     /locus_tag="ACHXWD_001677"
     CDS             5963..6631
                     /gene="folE"
                     /locus_tag="ACHXWD_001677"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR7620616.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6648..7805
                     /gene="yeiB"
                     /locus_tag="ACHXWD_001678"
     CDS             6648..7805
                     /gene="yeiB"
                     /locus_tag="ACHXWD_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR7620617.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7989..8987
                     /gene="galS"
                     /locus_tag="ACHXWD_001679"
     CDS             7989..8987
                     /gene="galS"
                     /locus_tag="ACHXWD_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR7620618.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            9267..10265
                     /gene="mglB"
                     /locus_tag="ACHXWD_001680"
     CDS             9267..10265
                     /gene="mglB"
                     /locus_tag="ACHXWD_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR7620619.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10326..11846
                     /gene="mglA"
                     /locus_tag="ACHXWD_001681"
     CDS             10326..11846
                     /gene="mglA"
                     /locus_tag="ACHXWD_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR7620620.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11862..12872
                     /gene="mglC"
                     /locus_tag="ACHXWD_001682"
     CDS             11862..12872
                     /gene="mglC"
                     /locus_tag="ACHXWD_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR7620621.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12999..14234)
                     /gene="preA"
                     /locus_tag="ACHXWD_001683"
     CDS             complement(12999..14234)
                     /gene="preA"
                     /locus_tag="ACHXWD_001683"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR7620622.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNTDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14228..15466)
                     /gene="preT"
                     /locus_tag="ACHXWD_001684"
                     /pseudo
     CDS             complement(14228..15466)
                     /gene="preT"
                     /locus_tag="ACHXWD_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(15660..15899)
                     /locus_tag="ACHXWD_001685"
     CDS             complement(15660..15899)
                     /locus_tag="ACHXWD_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR7620623.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15902..16621)
                     /gene="sanA"
                     /locus_tag="ACHXWD_001686"
     CDS             complement(15902..16621)
                     /gene="sanA"
                     /locus_tag="ACHXWD_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR7620624.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16771..17655)
                     /gene="cdd"
                     /locus_tag="ACHXWD_001687"
     CDS             complement(16771..17655)
                     /gene="cdd"
                     /locus_tag="ACHXWD_001687"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR7620625.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17785..18480)
                     /locus_tag="ACHXWD_001688"
     CDS             complement(17785..18480)
                     /locus_tag="ACHXWD_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR7620626.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18477..18875)
                     /locus_tag="ACHXWD_001689"
     CDS             complement(18477..18875)
                     /locus_tag="ACHXWD_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR7620627.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19114..20061
                     /gene="dusC"
                     /locus_tag="ACHXWD_001690"
     CDS             19114..20061
                     /gene="dusC"
                     /locus_tag="ACHXWD_001690"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR7620628.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4946 a         4998 c         5300 g         4979 t
ORIGIN      
        1 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
       61 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgaccgacct
      121 ggaagggcag cttggcgtgc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
      181 acacgggcgg ctgctctatc cgcgtgcgtt ggcattgctt gaacaggcgg ttgaaatcga
      241 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
      301 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga
      361 acttagcgtt gggaacagcc aggacgtgat gcaagcggtg ctggatttcc gcgttgatat
      421 tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga
      481 cgagctggtg gttttcgccg cgccgacttc gccgttggct cgtggtccgg tcaccttaga
      541 acagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggagattgt
      601 cgattatctg ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
      661 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt
      721 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tcccgcgcct
      781 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
      841 ctttctggac tattgcgatc ccgcgaatgt accgcgttaa ggtgctgtac aagaacatgc
      901 tggtgctgtg tcgatttcgt gacgcagcgc cctcagcatg cattcgccag aaaagagatt
      961 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     1021 ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg gatagcattt
     1081 ttgtatgggt tccgaaacta aaactacaga agcgccgggc ttacgccgtg aattaaaggc
     1141 gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc
     1201 tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg
     1261 cctgatggtt tacttcctga tgaccagtct cggtgaattg gctgcatata tgccggtttc
     1321 cggttcgttt gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg
     1381 ctggaactac tggtacaact gggcggtgac tattgccgtt gacttggttg cagcacagct
     1441 ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg
     1501 cgttatcttc ctgctgaact acatctcagt tcgtggcttt ggtgaagcgg aatactggtt
     1561 ttcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat
     1621 cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc
     1681 gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca
     1741 gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc
     1801 acgcgcggta cgtcaggtat tctggcgaat cctgttgttc tatgtgttcg cgatcctgat
     1861 tatcagcctg attattccgt ataccgatcc gagcctgctg cgtaacgatg ttaaagacat
     1921 cagcgtcagt ccgttcacct tggtgttcca gcacgcgggt ctgctctctg cggcggcggt
     1981 gatgaacgca gttattctga cggcggtgct gtcagcaggt aactccggta tgtatgcgtc
     2041 tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaatt
     2101 gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtttgtg
     2161 cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg
     2221 gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg
     2281 ttacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact
     2341 ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc
     2401 gttcctgaaa gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct
     2461 gttcctgatt atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag
     2521 cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct
     2581 ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt
     2641 aacatttgga ttgataattg ttatcgtttg cattatcgtt acgctacaat caaaaaaggc
     2701 tgacaaatca gaggctggtc cggctttctg ggatgatcac ctgcatacaa ataagtccac
     2761 cgcgatgctg ccgtacgcaa ggggacgtaa agaagatgtg agcgataccc attttatttt
     2821 cgtagttacc tcatggagat atggaatgtt taggttgaac cctttcgtac gggtcgggct
     2881 gtgtttgtcc gctatttctt gtgtatggcc tgtgttagcg gtcgatgacg atggcgaaac
     2941 gatggttgtc actgcatctt ccgttgaaca aaaccttaaa gatgcacccg ccagtatcag
     3001 cgtcattacc caggaagatc tgcagcgaaa accggtacag aatctgaagg atgtcctcaa
     3061 agaagtgcct ggcgtacaac tgacgaacga aggggataac cgtaagggcg ttagtattcg
     3121 tggtctggac agcagctata ccctgattct cgtcgacggt aaacgcgtga actcccgcaa
     3181 tgccgtcttc cgccacaatg atttcgatct gaactggatc ccggtcgatt ccatcgaacg
     3241 tattgaagtg gtccgtggcc cgatgtcgtc gctgtacggt tccgatgcgc tcggcggtgt
     3301 agtgaatatc atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta ccgtcgatac
     3361 caccattcag gaacatcgcg atcgcggtga tacctataac ggtcagttct ttaccagtgg
     3421 accattaatt gatggtgtgc tgggaatgaa agcttacggc agcctggcaa aacgtgaaaa
     3481 ggatgacccg caaaactcaa cgaccaccga taccggagaa acgccgcgta ttgaaggatt
     3541 ctccagccgc gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc acgattttac
     3601 tgccggatac ggtttcgacc gtcaggatcg tgattccgac tcgctggaca aaaaccgcct
     3661 ggaacgccag aactactccg tcagccataa tgggcgttgg gattacggca ccagcgaact
     3721 gaaatactac ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc cgataacttc
     3781 cgaaagcaat acggtcgacg gcaaatacac gttgccgctg acggcgatta atcagtttct
     3841 cacggttggc ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc tgaccggggg
     3901 aaccagctcc aaaacgtctg ccagccagta cgcgctgttt gtggaagatg aatggcggat
     3961 cttcgagccg ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa cctacggtga
     4021 acactggagt ccgcgtgcct acctggttta taacgccacc gacaccgtaa cggtgaaagg
     4081 gggctgggcg acggcattta aagcaccttc tctgttgcaa cttagccctg actggacgag
     4141 caattcctgc cgtggcgcat gtaagattgt gggtagcccg gatctgaaac cagaaaccag
     4201 cgaaagttgg gagctggggc tttactacat gggtgaagaa ggctggctgg aaggggttga
     4261 atccagcgtt accgttttcc gtaacgatgt gaaagatcgt atcagcatca gccgtacgtc
     4321 tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg gcgctaacgg
     4381 acggcgcata ccggtattta gctactacaa cgttaacaaa gctcgtattc agggcgtgga
     4441 aaccgaactg aaaattccgt tcaacgatga atggaaactg tcgatcaact acacctacaa
     4501 cgatggtcgt gatgtcagca acggcgaaaa caaaccgcta tccgatctgc cgttccatac
     4561 tgctaacggt acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc
     4621 tggtcactat accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta
     4681 taccatctgg aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg
     4741 cgtgctgaac cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg
     4801 acgtcgttac tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg
     4861 gcggactttc acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag
     4921 gcgatgaagt agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct
     4981 ttctggcgcg cggcttctgc caataccgca ggttgcaact ggtcggcaag aaactgatca
     5041 ttatcgccct gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc
     5101 gcgcaactat cccattccag ccatgcattt ttgtcctcac ctaaataggt gctaaacgct
     5161 ttgattcccc acgggacgct gcacggattc acaattggcg caaaggccga aacgctggtg
     5221 tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg
     5281 ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg
     5341 cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa
     5401 aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg
     5461 ctggtgtctg gcatcaccag tacaatcccc agttctgccg ccacgcgctg ggcacccgcc
     5521 ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc
     5581 ggggtgtgat cacgaggggg tgggagaaag atactgaacg tcatcgggca gtttaaggta
     5641 ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt
     5701 tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg
     5761 tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa
     5821 agtcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg
     5881 cacaataatc aggctgtaaa tcagcttaat aactttgccc ccacgcaggg cggaggcgtc
     5941 acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga
     6001 agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa
     6061 cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct
     6121 cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga
     6181 tgaaattttc tccggtctgg attacgccaa cttcccgaaa atcaccctca ttgaaaacaa
     6241 aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca
     6301 ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat
     6361 cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga
     6421 acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc
     6481 tgtctccatt gatgcggtgc attactgcgt gaaagcgcgt ggcatccgcg atgcaaccag
     6541 tgccacaaca acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga
     6601 gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc aggaaccatg gagcgcaacg
     6661 taacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg
     6721 cctttgggct accaaaggcg gcttatctca atcccgcctg gtacggcgct attacgccgc
     6781 aggatgcatg gacctgggca tttctcgatc tcatcggcca ggtgaaattc ctcacgcttt
     6841 ttgcgctgct gtttggtgcg ggcctgcaaa tgttgctgcc ccgtggcaga cgctggatcc
     6901 agtcgcggtt aacgctgtta gtcttgctgg gttttattca cggtttattg ttctgggacg
     6961 gcgatattct gctggcttac gggctggtgg gcttaatctg ctggcggctg gtgcgcgatg
     7021 cgccatcggt aaaaagcctg tttaatacag gcgtcatgct ttatctggtg gggcttggcg
     7081 ttttgctgtt attggggctg atttccgaca gccagaccag ccgcacctgg acgccggatg
     7141 catcggctat tttgtatgaa aaatactgga agcttcacgg cggcgttgaa gcgatcagta
     7201 atcgtgccga tggtgttggc aacagtttac tggcactggg cgcacagtat ggctggcaac
     7261 tggcagggat gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt
     7321 tcagcttacg tcactatcgt cgtactggtt ttgtgctggt ggcgattggg gtgaccatta
     7381 accttcctgc catcgccctg caatggcagc tggactgggc gtatcgctgg tgtgccttct
     7441 tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt
     7501 tttatggctt ctggccgcaa ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg
     7561 gacggatggc gctgaccaac tatctattgc aaacgctgat ttgtaccatg cttttttacc
     7621 acctcggttt gtttatgcat tttgaccgcc tggagctgct ggcgtttgtt attccggtat
     7681 ggctggcgaa tatcctcttc tctgttatct ggctgcgtta cttccgccag gggccggtgg
     7741 aatggctctg gcgtcagtta actttgcgtg ctgccggaac ggcaatatct aaaacatcaa
     7801 gataacgatc tggatcacat tcgttaacaa aacggctgta accgtttcca ttgctgtgac
     7861 tcgattcacg aagtcctgta ttcagtgctg acaaaatagc cgccagcaag cagtcattta
     7921 ctgcaatctc ataacaggta gtgaatatga tcaccattcg tgatgtagcg cgttaggctg
     7981 gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag cacgctggtc agtgccgaca
     8041 cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta tcggccaaac gccaatgccc
     8101 aggcgctggc aactcaggtt agcgacacca ttggcgtggt ggtgatggac gtttctgatg
     8161 cgtttttcgg cgcgctggta aaagcggtgg atctagtcgc tcagcagcat cagaaatacg
     8221 tgctaatcgg caatagctat catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa
     8281 ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc attgagtgac gatgaactgg
     8341 cgcaatttat ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg
     8401 cccatcgttg cgtttgcctg gataatctca gcggtgcccg aatggcgacg cgcatgttgc
     8461 tgaataacgg tcatcaacgt attggttatc tttcttccag tcacggcatt gaagatgacg
     8521 ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca ggatattatt ccgccggaaa
     8581 gctggattgg cactggtacg ccggacatgc cgggcggtga ggcggcgatg gttgaactgc
     8641 tggggcgcaa tctacaactt accgctatat ttgcttataa cgacaatatg gccgctggcg
     8701 cactgacagc attaaaagat aatggcattg cgattccgtt acatctctca atcatcggtt
     8761 tcgatgatat tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg cgttatccca
     8821 ttgcttcaat ggctaaatta gccaccgaac tggccttgca gggggcagca ggcaatattg
     8881 atcctcgtgc cagccactgt tttatgccga cgttagtgcg tcgccattct gtcgcaacgc
     8941 gccagaatgc ggcggcgatc actaactcaa caaatcaggc gatgtaaccg ctttcaatct
     9001 gtgagtgatt tcacagtatc ttaacaatgt gatagctatg attgcaccgt tttaacgttg
     9061 taacccgtat gtaacagtga ataatcactt tcaccgaggt aatagcgtca taacaacaat
     9121 taaagccgtt ttctggagcg ttaccgggca tggaagaacg aatttaaaaa agtgagcttc
     9181 ggcgttcagt aacacttcat taactctact gccccgccga gcatttatct caagcactac
     9241 cctgcataag aaaaaccgga gataccatga ataagaaggt gttaaccctg tctgctgtga
     9301 tggccagcat gttattcggt gccgctgcac acgctgctga tactcgcatt ggtgtaacaa
     9361 tctataagta cgacgataac tttatgtctg tagtgcgcaa ggctattgag caagatgcga
     9421 aagccgcgcc agatgttcag ctgctgatga atgattctca gaatgaccag tccaagcaga
     9481 acgatcagat cgacgtattg ctggcgaaag gggtgaaggc actggcaatc aacctggttg
     9541 acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt
     9601 tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc tactacgttg
     9661 gcactgactc caaagagtcc ggcattattc agggcgattt gattgctaaa cactgggcgg
     9721 cgaatcaggg ttgggatctg aacaaagacg gtcagattca gttcgtactg ctgaaaggtg
     9781 aaccgggcca tccggatgca gaagcacgta ccacttacgt gattaaagaa ttgaacgata
     9841 aaggcatcaa aactgaacag ttacagttag ataccgcaat gtgggacacc gctcaggcga
     9901 aagataagat ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg
     9961 ccaacaacga tgcgatggca atgggcgcgg tagaagcatt gaaagcacac aacaagtcca
    10021 gcattccggt gtttggcgtc gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg
    10081 cactggcggg cacagtactg aacgatgcta acaaccaggc gaaagcgacc tttgatctgg
    10141 cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac caactggaaa atcgacaaca
    10201 aagtggtccg cgtaccttat gttggcgtag ataaagacaa cctggctgaa ttcagcaaga
    10261 aataagactg attgggcgca gtcgtttact gcgcccttat tgtatggcca acaaggtaaa
    10321 attatatggt cagctcaacg actccgtctt ccggggaata cttgttggaa atgagcggta
    10381 tcaacaagtc ctttcctggt gttaaggcac ttgataacgt taatttaaaa gtccggccac
    10441 attctatcca tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc
    10501 tgtttggtat ttatcaaaaa gactccggca ccattttatt ccagggtaaa gagatcgatt
    10561 tccattccgc caaagaggca ctggaaaatg gtatttcgat ggtacaccag gagttaaacc
    10621 tggtattaca acgttcggtg atggataaca tgtggctggg gcgatatccc accaaaggca
    10681 tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc aatttttgat gaactggata
    10741 ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg
    10801 aaatcgccaa agcgttttcc tataacgcga aaattgtgat tatggatgaa ccgacttctt
    10861 cgttaaccga aaaagaggtc aatcatctgt tcactattat tcgtaaatta aaagagcgcg
    10921 gctgcggtat tgtttatatc tcgcataaaa tggaagaaat cttccagtta tgtgatgaag
    10981 ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc gctggcagga ctgacgatgg
    11041 acaagatcat cgccatgatg gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa
    11101 acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga
    11161 ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct cggtattgcc gggctggtgg
    11221 gggcgaaacg taccgatatt gttgagacgt tatttggtat tcgcgagaaa tcggctggca
    11281 ccattacgtt gcacggcaaa cagatcaata accataatgc caacgaagcc ataaaccacg
    11341 gatttgcact ggtaactgag gagcgccgct caacgggaat ttatgcctat ctggatattg
    11401 gttttaactc gttaatttcc aatattcgca actacaaaaa taaagttggt ttactggata
    11461 actcgcggat gaaaagcgat acccagtggg tgattgattc gatgcgggta aaaacgccgg
    11521 gtcatcggac gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg attattggtc
    11581 gctggctact aacgcaacca gaaatattaa tgctcgatga accgacgcgc ggtattgatg
    11641 tcggggcgaa gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga
    11701 ttattattat ctcctctgaa atgcctgagt tgttagggat aacagaccgt attctggtca
    11761 tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc
    11821 tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc catgagtgcg ttaaataaga
    11881 aaagttttct tacttacctg aaagagggcg gtatttacgt cgttctttta gttttgctgg
    11941 cgattattat tttccaggac ccaacatttt taagtctgtt gaacttaagt aatattctca
    12001 cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt gtcacccagg
    12061 ggaccgatct ttctgctggt cgtcaggtag ggctggcggc agtggtggct gcgacattat
    12121 tgcagtccat ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc
    12181 tggttattct gattgtctgt gccattggtg cggtgatcgg tttgatcaac ggtctgatta
    12241 tcgcttatct caacgtgacg ccgttcatta ccacgctcgg cacgatgatc atcgtctatg
    12301 gcatcaactc gctctattac gactttgtcg gggcgtcgcc aatttctggt tttgacagtg
    12361 gcttctctac ctttgctcag ggctttgtcg cgctggggag tttccgtctc tcttacatca
    12421 ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg
    12481 gtaagaacat ttttgccatt ggcggtaacc cggaagcggc aaaagtatct ggtgtcaacg
    12541 tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt gttctatgcc tttggcggga
    12601 tgttagaagc cggacgtatc ggctctgcca ccaacaacct cggctttatg tatgagctgg
    12661 atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg gggacggtga
    12721 ttggcgtggt gaccggggtg attattttta ccgtcatcaa ctatggcctg acgtatatcg
    12781 gcgtaaaccc atactggcag tacatcatca aaggggcgat tattatcttc gccgtagcgc
    12841 tggattcact gaaatacgcg cgtaagaaat gatgtgaatg tggtccgcat tcggtcataa
    12901 acgcacataa catcatgatc tgtgtggttt tgtaggccgg ataaggcgtt cacgccgcat
    12961 ccggcaacag gaaccaaatg ccggatgtga cgcgggttct acaacgttac cgggtgttct
    13021 ttctcgcctt tcttaaactt cacttccccg agctcaatac aacccaccgg gcagacgtga
    13081 ccacaaagca gacaacccac acatttctcg gtattacaat gcggtgtgcg ggttttctcg
    13141 ctccattcca tcgcctggtg accgccgtcg taacaggaaa tataacagcg tccacagcca
    13201 acacatttgt caagattgat acggggatag acaatataac tgcggtctaa atcttccgcc
    13261 gggacaatat tgttattcgc cagacctacc atctcctgca acgaatcaaa tccctgatcg
    13321 gcgagataat gcgataaccc gctcgccata tcttctacga tgcgatagcc gtactgcata
    13381 atgccggtgg tgacctgtaa cgttgctgcg ccgagcaata aaaactccgc cgcatcctcc
    13441 caggtttcaa tgccgccgat accgctgatt gggaaatcgc gcagttctgg atgggtgcgc
    13501 atttgctgaa tgaagcgcag ggcgatcggt tttaccgctt taccggaata tccggaaata
    13561 cttgattttc cgttaacgat cggcataccg attttctgat taagatcggt attggtgatg
    13621 gatttaacgg tgttaatcgc cgcaatgcca tctgcgccgc cgcgcttcgc cgccagcgcc
    13681 acttcgcaca tatcaccgat attcggcgtc atcttcgcca gcattggcag cgtggaaccc
    13741 cgtttcactg cccgacaata tttttctacc agctccgggc tttgcccgac atcgctaccc
    13801 atcgcatgag aagtcatttg cggacaggag aagttacact cgatcatatc cgcgccagct
    13861 tcttgcacca ggcgcgccag ctcctcccat tgctgctcat tttcccccat gatcgaagcg
    13921 atcaatactt tgtccgggta atcttctttc agccgacgca gggcggccag attctcctcc
    13981 agcggatgct cggcaatctg ctccatattt ttgaagccga taaaaccggt atcttctttc
    14041 accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat cgttttaaac
    14101 accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca gttgcctacc
    14161 ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaagt aatcgaaaga
    14221 tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg gcggcttctt
    14281 tcccggtttt cacggcatag accacggttt tgtctccctc aacaatatcg ccagcagcaa
    14341 agacttgcgg gtcgcgggtc tggtaatttt gtgttttgat ggtgttacgc tgcggctcta
    14401 actccgcaaa ggcatctagt ctggcatgct gaccgacggc gagaataatt ttatctgccg
    14461 ccatcgtcag ttcgcccgat aaccgtacat gcttaaacgt gactttattg ccttccacgg
    14521 ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg gtaaactctt
    14581 tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc gcctgacagc
    14641 cgagaacttt cagcgtgctg gctacgtcca tcgcgacatc accgccgccg ataattaatg
    14701 cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag aagtcgacgg
    14761 caatctcaac gtcactatgc tcaaacagcg gtagcccgga accgcttgat aaccccacgg
    14821 tgaccagtac cgcgcggttt tctgctttca gctgctcaag ggtgagtgtg ttaccgactt
    14881 cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct gcatccagta
    14941 ctgattgcgg taaccggaat tgcggaatgc cgttacgcaa tcagccaccg ggttgtgctt
    15001 ctttctcata gatcgtgacg tcataaccct ggtttgtcag tgtcacactg gcctgcaatc
    15061 cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg ggctgataaa
    15121 tttccatccc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg ccgatatcaa
    15181 tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc gtcgggcaca
    15241 ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca gcgcctttaa
    15301 aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc gggcaagcct
    15361 gactacaggg agcgtcatga cataataaac agcgagaggc ttctttaatc gccagtaaag
    15421 gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga tcttccttaa
    15481 tgctatgagt aattaaggcg aacgctaaat aaatattgcg tgctgatatt aaggctaagc
    15541 gtgtggggaa atttaaactg tgaaaggatt cacaaatagc tgcggttcag aatgttatat
    15601 ccgcagctta ttgagtgaag taataattat tctcaataat taacagaaaa gagaaaatat
    15661 taacgataga aaataagata aacaatcatg ccgatagata aaattccgaa gccgatatat
    15721 gccaccatat aagcgaaaaa gagtccggga ttttttgctc gccagacata caccggttcc
    15781 ggtgcatttt taacccgttt atcaatcgcg cctgcgcgcc agcctttcca tgcttcgcgg
    15841 aaacaaaata tcgggataag gaaaaaaacg gcaacgacaa aaaactgctg tacatccata
    15901 actactttcc ttgtttcttt tgtaattcaa gtaactgttc gggtgtgacg gcgggatacc
    15961 cctgcgcatc ttccggcacc tggtgcatag ccggaatagg gaccagcggc cctaaaaaac
    16021 gcggttcacg tctaaaaata taaaggtcag ccagcgcacc gaaacgggcg gcaaattcgc
    16081 gaatacgtac tgacagcata tctttcggtg acggtacggc ataacactga gcctgaatcc
    16141 ccatatgcag cgcaataaat aatgcccgct cacagtggaa acgttgggtg ataataatga
    16201 aatcattagt atcgaacact ttgcgtgtac gcacgatgga atccagcgta cgaaagcctg
    16261 cgtaatcgag aacaatatct gatgggtcga caccagcagc gattaaatct ttgcgcatgg
    16321 tcatcggctc attataactt tgcaatgcgt tatcgccgct cagtaataga taatttacct
    16381 taccgctgtt ataggcatta atcgctcctt gaatgcggta gcgataatac tgattaatta
    16441 cgccagtacg ataatatttt gctgtgccga gcaccacgcc gacctggcgg taggggagat
    16501 cctgcaattc gtcgtagata taaggcgcgg ttttccagct catccagcga tcgaggccga
    16561 gcacagtcaa cagcagcaag ccgatcagga ctaacaggct gaggaacacg cgctttaaca
    16621 tgcaggagac tcgatctaag gaaaatttcc atcaggctac tttacccgct ggttttgcgc
    16681 aagaaacggc aaactgtttg ccgggaattt cgacaccaag agagggctgg caagccggac
    16741 aaggcgttca cgccgcatcc ggcaccaggc ttaagcgaga agcacgcggt cgatactgtg
    16801 acagccgaga gctttcagcg ttgcggaggt ggcatcccac tgaatcaacg gcgcatcggc
    16861 tttttctgcc agaaccgcgc gctggatatc cgggtaatca taacccttga gattcaacag
    16921 aattaacgcc ccctgcaacg gtggcagagt cgggttgaat gcggcgtttt cagcgtagct
    16981 gccgctgaaa atacgaccgt ctttacattc cagcgcgaca ccgcttggcg acttactgta
    17041 aggcatgtgc gaacggtttg ccgccgcaat cgctgcctga gaaagcgcat cacccgtcag
    17101 cgcatagccg tgatcctgtt cgtccatcag cagcgtttta atctccagat ctttcggccc
    17161 aaaggcatcc ggcagatagt cacgcagcgc gtgtgcctcg cggcccggca gatgaatacg
    17221 cagatccaga ccgctgttca gttcattcat aaactgacgg cagtgaccac aaggcgtata
    17281 gttaacggtg atggctgcaa gcgctttttc accactcaac caggcgtggc tgatcgcgct
    17341 ttgttcggca tgaacggttt gctgcattgt tgcaccaata aattccatat tggcaccgaa
    17401 ataccaggtt ccgctcacac cgcgcgcaat tgcgccaaca ttaaaattcg acaatggcgt
    17461 acgcgcacag gccgccgcca gtggaagtag tgcgaatgcc agcgcgtctt cgtccagccc
    17521 cgttgcgctc ttcagcgatg agacttgctc cccggtcaac aaagcgggga agtacttgtc
    17581 tgccagaata ggttccagtg cagattgcaa gttatccgca agttgggcaa aagcggtttg
    17641 aaaacgtgga tgcatggcgt tgcctcataa tcatgtaatg ggataacagt ttacgaaccc
    17701 gttatggctt tatatgtgat ctgaatctca ttaattatgc aacgcgtgaa acttacatca
    17761 aaaatgcgcg acgcatcgca aattttagcc cattactgcc agaataatcg ggaaaaggaa
    17821 cggtgcgatc agcgaagtaa ttatcccgca taacaccagc gccagcgaac taaatgcacc
    17881 ttcctgataa tccagctcgg cgcagcgcgc cgtaccgagg gcgtgcgagg ctgtccccat
    17941 tgccagaccg cgcgcagctt tggtacgaat acgcatcgca ttaagcaatg tatggccaaa
    18001 taccgcgccg aggatgccga cgaaaatcac gcaaactgcg ctgattgccg gaataccgcc
    18061 aatactgccg ccaaccgcca ttgcaatagg cgtggtgact gatttcggca ggattgacgc
    18121 ggcgatttcc ggtgaagcgc ccatcaacaa tgccacggaa gtccccgtca ccatcgcaac
    18181 cacgctgccg ataaaacaga tggtgatgat cgatttccag cgcgcgcgga tctggtgtag
    18241 ctgctcatat aaaggatagg ccagcgcgac gaccgccggt tgcagcagat cgtttagcac
    18301 ttcgctacct ttaaagtagc tgtcgtaaga gatgccggtc agcattaaaa aaggaatgat
    18361 caccaccatt gctaccagca acgggttaag caggggaaac ttataccgtg ccgccagttt
    18421 gcgggcggca aaaaagacaa tcaacgtcag cggtaatgac caccagatat tcgccatcat
    18481 tcttctgatc ctttctgacc tacgacttta cgttcaccgt gtaccagttg tgaactccag
    18541 ctcaccacca gaaaaaccac cagcgtactg actgcacagg aaaccactac cgggccaaac
    18601 tgtgcgcgga gcaaatcaaa atattgcatg acgcctacgc caatcggcac aaacaatagc
    18661 gccatatagc gaatcagtac gtagcacccc ggattgaccc attttgccgg aagaatttgc
    18721 aaggccagca ggacaaacag gatcagcatc ccgatgatgc tgcccggaat ggttaccggt
    18781 agcagggaag caatgaaaat gcctgcatac aggcaggcat aaatcaggac gaaagcgcgt
    18841 aaatattgcc agataatgtt cagtgtctta ctcataatca acaccccaga tgagatggat
    18901 tcatcataca attaattcgt aaaacgtgct accgatcaca ttaagaaatc agcgcttatt
    18961 ttttcgctcc ccggcattac gcaatgcgca acgaactcag gcatacatca agcatagaga
    19021 gggattacag tttgcagcaa tccctctttg cagaccttta atccgttgac cataggcgct
    19081 aacatagcgc ctcatttttt tccgggtgaa gatatgcgtg tgttactggc tccgatggag
    19141 ggtgtgcttg actctctggt gcgagaattg ctgaccgaag ttaacgacta cgatctgtgc
    19201 atcaccgagt ttgtccgcgt ggtggatcaa ctgctgccgg taaaagtctt tcatcgcatt
    19261 tgccctgagc tacaaaacgc cagccggaca ccatccggta cgctggtgcg cgttcagttg
    19321 ttaggtcagt tcccacaatg gctggcagag aacgccgccc gtgcggtgga attaggttcc
    19381 tggggcgtgg atctcaattg cggctgcccg tcgaaaacgg ttaacggcag cggcggcggg
    19441 gcgacgttac tcaaagatcc tgaactcatc taccagggtg caaaagcgat gcgtgaagct
    19501 gtaccggcgc atttgcccgt cagcgtgaaa gtgcgtctgg gctgggacag cggtgagaag
    19561 aaatttgaaa tcgccgatgc ggttcaacag gctggtgcta cggagctggt ggtgcatgga
    19621 cggacaaaag agcagggtta ccgcgcggag catattgact ggcaggcgat tggcgagatt
    19681 cgccagcggc tgaatattcc ggtgattgcc aacggcgaaa tctgggactg gcagagcgcg
    19741 caacaatgca ttgcgatcag cggctgcgac gcagtgatga ttggtcgcgg ggcgctcaat
    19801 attcccaacc tgagccgggt agtaaaatat aacgaaccgc gaatgccgtg gccggaggtg
    19861 gttgctttgc tgcaaaaata tacccgtctg gaaaagcagg gcgataccgg gttatatcac
    19921 gttgcgcgga ttaaacagtg gttgagttat ttgcgtaaag aatacgatga agcaacggaa
    19981 ttatttcagc atgttcgggt gttgaataat tcccctgata ttgcaagggc tattcaggca
    20041 attgatatcg agaaactcta aaaaaccata gtgatgtttt ttgacaatgt gttagctctt
    20101 tatcaatagg ccgcacggtt gtttggtggc gtcaaaattg ccgaggcgtt ccctgaaggc
    20161 cggggcagcc cacatggagg tgggctgagg gcgcgtttta cagggatgtt acctcgcgcc
    20221 cga
//
LOCUS       DAZSBF010000059        20148 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZSBF010000059 DAZSBF010000000
VERSION     DAZSBF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.060"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHXWD_001691"
     CDS             complement(<1..1787)
                     /gene="ygfK"
                     /locus_tag="ACHXWD_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR7620629.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
     gene            complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHXWD_001692"
     CDS             complement(2109..2687)
                     /gene="mocA"
                     /locus_tag="ACHXWD_001692"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR7620630.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHXWD_001693"
     CDS             2790..3560
                     /gene="yqeC"
                     /locus_tag="ACHXWD_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR7620631.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHXWD_001694"
     CDS             3608..5233
                     /gene="yqeB"
                     /locus_tag="ACHXWD_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR7620632.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHXWD_001695"
     CDS             complement(5274..6206)
                     /gene="arcC"
                     /locus_tag="ACHXWD_001695"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR7620633.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFVRSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHXWD_001696"
                     /pseudo
     CDS             complement(6254..7638)
                     /gene="hyuA"
                     /locus_tag="ACHXWD_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            complement(7691..8902)
                     /locus_tag="ACHXWD_001697"
     CDS             complement(7691..8902)
                     /locus_tag="ACHXWD_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR7620634.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHXWD_001698"
     CDS             complement(8960..10156)
                     /gene="dpaL"
                     /locus_tag="ACHXWD_001698"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR7620635.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHXWD_001699"
     CDS             complement(10214..11401)
                     /gene="ygeW"
                     /locus_tag="ACHXWD_001699"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR7620636.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            11880..13658
                     /locus_tag="ACHXWD_001700"
     CDS             11880..13658
                     /locus_tag="ACHXWD_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR7620637.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHXWD_001701"
     CDS             complement(13698..14177)
                     /gene="xdhC"
                     /locus_tag="ACHXWD_001701"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR7620638.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHXWD_001702"
     CDS             complement(14174..15052)
                     /gene="xdhB"
                     /locus_tag="ACHXWD_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR7620639.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHXWD_001703"
     CDS             complement(15063..17360)
                     /gene="xdhA"
                     /locus_tag="ACHXWD_001703"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR7620640.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            17775..18530
                     /gene="actS"
                     /locus_tag="ACHXWD_001704"
     CDS             17775..18530
                     /gene="actS"
                     /locus_tag="ACHXWD_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR7620641.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            18609..18678
                     /locus_tag="ACHXWD_001705"
     tRNA            18609..18678
                     /locus_tag="ACHXWD_001705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18641..18643,aa:Gly,seq:ccc)
     gene            18883..>20148
                     /locus_tag="ACHXWD_001706"
     CDS             18883..>20148
                     /locus_tag="ACHXWD_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR7620642.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKR"
BASE COUNT         4981 a         4765 c         5179 g         5223 t
ORIGIN      
        1 aactgaggaa tgatattttt caccacgccg ccagcattgg cttcgcgttc aaacagcgta
       61 accggatggc ccgctctggc aaggaagtaa cctgctgcca gacccgccgg acctgcacca
      121 atcacggcaa ccggatggcg tgaaccagaa ccggcgggtt tgtgccagcg ttgcttatat
      181 tcatcccaac ctttttccag cgcgactttt ttcagttcgc ggatattcag cgcactgtcg
      241 taatccaggc gggtacagtt gtattggcac tggtgatcgc aaatatgacc ggtaatggca
      301 ggtagggcgt tgcgttggta gatgagttcc agcgcgtcgg catagcggtg ttcgccaagc
      361 agacggatgt attccggaat atcttgcttg atagcgcagg cagtaacaca gggggcaacg
      421 tagcaatcgg tcagcggcag gtcttctgcc acttcaatac gctcttctgg cttccagtgt
      481 ttctgggtgt attccatggt taacgcgtct gctgccagtc tgttcagtcg ttcgacgtca
      541 acatggtcaa gtccccaggc gtcggagcct tccagctcgc gcatgcaggc acttaagcgc
      601 agatagccgc caggtttcag caggtcggtt gccatagtaa tagggcgaat acctgtatca
      661 aaaatatcgc ggatagtcag ctgactggca ccaccggaat aagaaatggg cagtttgccg
      721 tcaaaggcgc gagagagaac tgctgcaaca ttgatggaga gcgggaacag cgcacggcct
      781 gacatataca tctcttcacc aggcagtgcg cctttattgt tgatggtgcc gagagtgtta
      841 gtcagtttta cgccaaagcc gagtgatttt tcttttgcca gtaccatcag gcgctccagc
      901 atttccagtg cttgcgtcag cttgaggtcg tgatcaaatg actcttcttt taagcctatg
      961 taaccgaaac cgcagacatc gaggatctca cgaacacgcg cgtaccccag taaggtcggg
     1021 ttaagtttga caaaggtgtt gagccctttt tcttccagca tgtagcggca aatggcttcg
     1081 atttcatgcg gaggacagcc gtgcatggtg gagaggggga cgccctgcac catactggtg
     1141 gggatgcgag cgggtaaggc ttgcaagttt tcgcgttttt cctgcaatcc gtggcgggct
     1201 aaaaatgcgt catcctggag taatttattc agcgtatcgc gatattgagc gaatttcgga
     1261 tggtcagatg cgtccatcat attgtcgatg aactgttgca tcggcggttg cttaatacct
     1321 tcgaggttgt aaccgatgct catattaaag atgaacgatt taccggaatc agaaggctgt
     1381 aacatcgctt cgagaaggtg cagggcaaac caggctttga ggtattcatc ccaggcttta
     1441 agcaaggtaa attcggtaga ccattcggtg ttaaagcatt cgtcttcggc atcaatacag
     1501 ggcttttcca gctccaggcg gtcaagaatt tggacggttt ttagttcgat gaagcgtccg
     1561 ccagtcagcc aggaagtgac aatattttgc gcgagctgcg tgtgcgggcc agcggcaggg
     1621 ccgacgggag tggcacaggt ttcaccgaag acgctaacag ttttaccttt tacaggtgag
     1681 taaaactgtt gctcgggaat accaaagatt gagcgttgtt gttggtattc atcaaatatg
     1741 cgcgtcaaaa gttcctcaaa cggaatggga cgcataatat cccccataac cctctcctta
     1801 gcttatacag atatcattga gtgaccggtg ttgagaaatc cggtggttta gggggataga
     1861 gcaacaatcg caccactctt gccaggtttg gcgggaaata taaaaaaatg tgaggtggct
     1921 cgaaacttaa aggctttttg tgaatgaact cacaggtgcc aaatgagaat taaaatcact
     1981 cctgaaaatt attattgtca gcccatttat cactttttct cttttctcaa aatcagtgat
     2041 tccttatcat tatgataaag agtgctggag agattaaata tttgaacgca atcgtttaac
     2101 tacattttct aaattttagt ataccttttt tgcgctgtaa taaagtcgtc cggagtatca
     2161 atatctaaaa ttatttctgc attttctatt tcgacggaat aatgatctcc cataaggagt
     2221 gcctgacgca tattggtgac attgggtcgc tggattgcct gcatcaggca tggttttgac
     2281 actaaaatcg gatggccggg gatgccatta tggagtggca gtattgcgcc atcatttcgt
     2341 aacgaccaga tttttctaaa aatatcgatg gtgagggttg gcatgtcacc gtgggtgaga
     2401 aaacaatgtt ctgtttgcac tgcgggtact gcggccttga cggatgttag taaaccctgc
     2461 gcataatctg ggttgtgaat aatagtgata ttgctctggt tcgcataacg ttcgtgcagt
     2521 tcattaccac gatagccggt gactaaaata attcggctac aaaactgcaa cgcatttttg
     2581 atacttgtat caagaattgt tccctgttcc caggggaaca tcattttcca ttgccccatt
     2641 cttgatgata atccggcagc ggtaattata cagtcgatgg ctgacatagt ggttccatta
     2701 tttatagggg ctaataacgc ctgataattg cgttacttcc tgatggatta agttggggat
     2761 aatttatccc agagaggtca taaagactcg tgaaaagtat agttgaccca tcggcattag
     2821 tcattgattt aggtgcgcag aaacgtccca ctgtgatttc tgttgttggt gctgggggga
     2881 aaaccagctt gcttttttgg ctggcagaac tgctccaggc tagcggcagg cgtgtgttaa
     2941 tcacgacgac gacacatatg tttatgccaa cgtctcactg gcccgtggtt ttctgtcgtg
     3001 atcccgccat gcttcctcat gcgtctctta catcccccat ttcattttgt tttcacagct
     3061 ggaaagcgaa ccagggaaaa gtgcagggat ttacgccaga agcgattgat gcactggtgc
     3121 aacgaccaga gtgtgacgta attctcattg aagcagatgg ctcgcgtgga atgccgttaa
     3181 aagcgcctga tgagcacgaa ccttgcatac ccaaaagcag ttgctgcgtg attgctgtga
     3241 tgggaggaca tactttgggc gcgaaagtga gcacagaaaa tgtccatcgc tggtcgcagt
     3301 ttgctgatat taccgggtta acacctgatg caaccttgca actgagcgat ctcgttgcgc
     3361 tggttcgcca ccctcagggg gcgtttaaaa acgtaccgca aggttgtcgg cgagtctggt
     3421 tcattaaccg tttttctcaa tgtgagaatg cgattgcaca aagcgagctc cttcaaccgc
     3481 tgcaacaaca cgacgtagag gcaatctggc tgggcgatat acaagaacat cctgcaatcg
     3541 cgcgcagatt tgtgaattag cggcccgggt attccgggtt actgaaagga tatccattga
     3601 ggttggtatg aatattttca cagaggctgc aaaactcgaa gagcaaaatt gtccgtttgc
     3661 gatggcgcaa attgttgata gccgaggctc gactccccgc cattctgcac aaatgttagt
     3721 gcgcgccgat ggttctatcg tcggtacaat tggtggcgga atggttgagc ggaaggtgat
     3781 tgaagagtca cttcaggcat tgcaggaacg taagccgcga ttattccatg gacgtatggc
     3841 tcgtaacggt gcggatgctg tcgggtcaga ttgtggaggt gcaatgtcag tatttatcag
     3901 cgtccatggt atgcgtccac gtctggtgtt gattggcgcg gggcatgtca accgggcgat
     3961 agcccagagt gcggcgctat taggatttga tattgccgtt gccgatattt atcgcgaaag
     4021 cctcaatcct gaactattcc caccatcaac cacgcttctc catgctgagt cgtttggtgc
     4081 ggcagtggaa gaactggata ttcgccctga taattttgtc ctgattgcca cgaataatca
     4141 ggatcgtgaa gccctcgaca aactcattga acagcccatt gcatggttgg ggttgctggc
     4201 aagtcgccgc aaggttcagc ttttcctgcg tcaattgcgt gagaaaggcg tggctgaaga
     4261 acatattgcc cgtttacatg cgcccgttgg ttacaacata ggtgcggaaa cgccgcagga
     4321 gatcgccatc agcgtgctgg cagaaatatt atgggtgaaa aataacgcgc cgggtgggct
     4381 gatgatgaaa ccttctcatc cttccggaca ccagctggtg gtgattcgcg gtgcgggaga
     4441 tatcgccagt ggtgtggcgc tacgtctgta tcatgcgggt tttaaagtga tcatgctgga
     4501 agtggaaaaa ccgacagtga ttcgttgtac cgtggcgttt gcccaggccg tgttcgatgg
     4561 cgaaatgacg gtcgaaggcg tcactgctcg cctggcaacc agctctgcgg aagcgatgaa
     4621 acttaccgaa cgcggattca tccctgtgat ggtagatccc gcctgttcat tgcttgatga
     4681 actgaaaccg ctttgcgtgg tggacgctat tctggcgaaa cagaatttgg gaacgcgggc
     4741 agatatggca ccagtaacaa tcgcgcttgg gccgggcttt actgcaggga aggattgtca
     4801 tgcggtaatt gaaacaaatc gcgggcactg gctcggtcag gtgatttact ctggttgtgc
     4861 gcaggagaat accgatgttc ctggcaatat tatggggcat accacccgac gggtgatccg
     4921 tgctcctgct gcaggcatta tgcgatccaa cgtgaaatta ggcgatctgg tgaaagaggg
     4981 cgatgtgatt gcctggattg gtgagcatga aattaaagca ccgttgacgg ggatggtgcg
     5041 tggcttgttg aacgacggcc tggcagtggt tggtggtttt aaaattggtg atatcgatcc
     5101 tcgtggtgaa accgctgatt tcaccagcgt ttctgataaa gcccgggcga ttggcggcgg
     5161 cgtacttgag gcgttaatga tgttgatgca tcagggcgtg aaagcgacaa aagaagtgct
     5221 ggaagtggct taaatgaaaa tggccggatg gtattaccca cccggcctta aaattactga
     5281 caaatcaccg ttccggtttt gccttcaatc ccttctttcg ctttgctcaa tacggtaatc
     5341 agtgcttcac gacctgcgcg agagcgaaca aacgaggcgg cagcttccac tttaggcagc
     5401 atagaacctt tcgcaaaatg accttcttcg ataaagcgtt cagcatcact taatgacagg
     5461 cgatcgagcc actgttcatt ctctttaccg aagttaatgg ccactttttc taccgcagtc
     5521 agaatgatca gcatatcggc atcaatcatt tctgctaaac gggcgctggc ccagtcttta
     5581 tcgataaccg cgctggcacc acgcagatgg ttaccttcac gaataacagg gataccacca
     5641 ccgccaacgg cgatcaccac ctggcctgca tctaccagag ctttaaccgt ttctttttca
     5701 atgatatcaa ccggttttgg cgaggcaaca acacggcgat agccgcgacc agcatcttct
     5761 ttcagagtgt aaccctgttt tgtcagttgc tctgcttctt gctcggtaaa gaacgaaccg
     5821 atcggcttgg tcgggttgag gaatgccgga tcgttagcgt cgacttcgac ctgagtaacc
     5881 agcgttgcta caggtttatt aatgccccga gaaagcagtt cttctcttaa cgcgttttgt
     5941 agatcgtaac caatataacc ctggcttaag gcaacacaga cagacatcgg cagcattggc
     6001 gagtgtgctt cagtttttgc ggcagcttca aaggcttggt taatcatgcc cacctgcggc
     6061 ccgttaccat gagtgacgat aacttcatgt ccttgggcaa ttaaatcaac aattgcctga
     6121 gaagtaattt ttaccgcttt catttgtccg gccaggtcat cgcccagcgc attcccgccc
     6181 agggcgagaa caattttctt actcattttt cctcactcat cattcttgat tgtgtataga
     6241 tccctgacag tggttagagc acgggaggga caaacggttt gcgtcgcagg aaacgacctc
     6301 gcccggcttt gcctgtaaag gtgccgtcac agaaaatcgt ttcaccacgg gataaggttc
     6361 tgacaatcgc gccctgacag gtaaaaccct cccatggcga gtagtcggcg ttgtcgtgga
     6421 gatggcgatg ctgaatttgt tggctctgac gtgggtcgat aatcaccacg tcgccatcgg
     6481 aaccgggcgc taataatcct ttttgcggcc acaggccaaa cagcctggcg ggcattgcgc
     6541 tggttaattc aacaaagcgt tccggtgtta tacgtcccgt catcacgcca ctggaaaaca
     6601 gtaactgcat gcggttttcc acaccgggta agccatttgg gcagcgactg aaatcgcctt
     6661 tagaaatttg caggcgttga gccatcgaga aggtgcagtg atcggtcgcc accacgtcaa
     6721 tcgcaccatc gctgatgcca caccacagtt tgtcctgctc gcgtacgtta cgcagcggtg
     6781 ggctaagaat gaacttcatg ccatcttctg tatcgtaact gcgttcgtcc aacaggagat
     6841 attgtgggca ggtttcaacc cagactggct ggtgattcgc acgggcaaga cgcagataat
     6901 ccagacctaa gccgttagac aggtgcacga tatagagcgg ggcgttaccg gcaatttgtg
     6961 ccaggttaat catgcgggcg atggcttccg cttcgcattc cagagggcga ctcaaggcat
     7021 gatagcgcgg cgcggttaac cctgcggcga taaattccgc ccgcttgctg gcgatagccg
     7081 catcattttc cgggtgcacg gtggtcagcg cgccggattc atgcaagcgg cgtaatgcct
     7141 gcaaaacctc gtcatcgttg agtttgtatt gataggttaa atagagttta aaactgctca
     7201 gtccttcctc gaccatcatc gggatttcgt cgaggattgc gtgattaatg tgctggatca
     7261 caccgtgaaa gctgtaatcg atgaccgctt tatgggcggc ataaccacga taaacctcca
     7321 gttgatggcg taaccgacag ccgtttgggc caaatcccat atggtcaata atggttgttg
     7381 taccgccaca cgcagctgcg cgggtaccgg taaaaaatca tcacaactgc gcgcgatgcc
     7441 gacatcaata ttgaaatgcg tatggacatc cacgccgcca gggaaaacgt aacagccagt
     7501 ggcatcaatt tcttcatacg ggagctgcgg cgaaatattg ttgcccaact ggcgaacaat
     7561 cccgctttca atcagcaaat cctgtttggc ttgtccatct gcgttaacga cagtgccgtt
     7621 tttgatcaat acgcgcatag caaactccaa aaaccctccg gcgaaccgga ggggaataag
     7681 tgaagagtaa ttactctgtt gccagccagc ttaacgggat tgcagcgtac atcgcggcac
     7741 aggtcaccag gtgagatttc caggtttttt cgttaggtgc atgcgcttca ggttctttac
     7801 ccgggccaaa gccgatcacc ggaatgccgt gacggcccat gatagatacg ccgttagttg
     7861 agaaggtcca cttatcaaca accggcgctt tgccaaacag cccttcgtag gcattcacca
     7921 gtgctttaac ggtgaagtga tcttcttcca ctttccaggt cgggaagtag cattcggttg
     7981 ggtaaaccag gccagtccag gacggacggt cgtagttgta catagaaaca accgcgttag
     8041 ctttctgtac tgcaggcagg gcgcggattt cgtccagcgc gccttcccag gtttcgcccc
     8101 aggtcagacg gcggtcaata gagacggcgc agctatctgc tacagcgcaa cggcttgggg
     8161 atgtgaagaa gatttcagaa acggtgaggg tgcctttgcc gaggaattcg tcataaccca
     8221 gacgttggga gagttcttgt aattcgccaa gaatcggacc cattttgaaa atggcgttgt
     8281 caccgcgttc tggcgcagaa ccgtggcagc taacaccctg aacatcaata cgaatttcca
     8341 tacgaccgcg ctgaccacgg tatatctggc agtcggttgg ttcggtactg accacaaatt
     8401 ccgggcgaat gccggattgt tcaataatgt actgccagca cagaccgtcg cagtcttctt
     8461 cctgcacagt accggtaacc agcagggtat attcatcttc cagaccgagg tctttaatga
     8521 ttttaccggc ataaaccata gatgccatgc cgccttcctg gtcggaagta ccgcgaccac
     8581 cgattagctc atcagtttcc atgccttcgt acggatcgaa gtcccagttt ttgatgttgc
     8641 caatgccgac ggtatcgata tgagcgtcca ttgccaccag acgcgggccg tggccgatat
     8701 aaccgagaac gttgcccatc gggtcgattt caactttatc gaagccgact ttttccatct
     8761 cttctttaat acgatgtact acgcgttttt cgtcgcagct ttcactggga atagcaacca
     8821 tgtcgcgcag gaagcgagtc atgtccgcct ggtaatcttt tgctttttca agaatcagtt
     8881 tgaatggaat attcttagcc attatttttc tccagatttt ttactccccg ttttgcaggg
     8941 agtagttcca atagggtgat taaggtgcta cagcgtgttt gccttcccag acaacttcgc
     9001 ggtagtgctt cacgtcggtg tcgccttcag tgctgataac cagcactacg gcatctttgt
     9061 ccagcgccag tttttccatc aggctttgat gttgcgggtg ataatgaacc gctgcgagaa
     9121 cgcccaaacc gacagcgccg gattcaccgg agatgatgcg cgggtcgttg ccgtacggat
     9181 tacccagcac gcgcatacct aatgcggcaa cgctgtcctg gcaggagatg aattgggtgg
     9241 cgcagttacg taggatttcc cagcccagcg ggttaggttc gccacaggcc aggcccgcca
     9301 tgatggtggc catatcaccg ccaacgttga cgatgtcgcc tttgacgccg gagcgataaa
     9361 tacagtcagc tttgtcaggt tcaacaataa tgctgtgcag attttgcggg ctgtagacgt
     9421 cgaccagata acccagcaca ccaccggcca ttgctccgac accggcttgc agcagaacgt
     9481 gcgtcggggt tacgcccatt tcacgcattt gctcgacggc ttcatctgcc agggttgcgt
     9541 agccttgcat gatccaggtt gggattttgg tgtaaccttc ccatgccgtg tcctgtacca
     9601 cttcccagcc gtgctgctgc gcgtgttgca tggtcaggcg aacggtatcg tcatagttca
     9661 tatccgtgac gatgcactcg gcaccgaggt tcagaatggc gtcaacgcgt tcctgagcag
     9721 aacctttcgg catgtaaatc accgcattct gtccgagttg ctgtgctgcc cacgccacac
     9781 cgcgcccgtg gttgccgtcg gtggtggtcg cgaaagtcat tttttcgccg atggcatttt
     9841 tcaggtgctc aaatgacagc gtttcgatat caagatgata tttttcacac aataattgag
     9901 cgatggcgta cgcaccgcca agcattttga aggcgttcag accgaatcgt tttgattcgt
     9961 ctttgacgag aattttcttc acaccaaaaa ggtttgcgag atcgtccagc gcacaaagcg
    10021 gtgttgggcg ataaccagct attttctggt ggaactggcg cgccagtttg gcctggcttt
    10081 gcgaaaagag cggtgatgtt tcgccgttga aaaatttgtt atcggcgata tcaatcttca
    10141 atgagaaaac ggacatatcc tatccttttg gcctctggaa atagagggcc gaccagcaga
    10201 tcggcccgag gggttatttc acgcgttctt gcgcttcttt cagcagttgc tcgagcagtg
    10261 cacctggttt ggcgtattta cgggacagga tcatcgcggc gatgatataa ggcttccagc
    10321 tggcttcttt gtaggtagcg atacggtatt tttcgaatac gccttcagtc acttcacctt
    10381 ctttgcagga tacgccgctg atatcagctg gcaggcagtg catgtacagg gcttcgccat
    10441 cacgggtcag ttccatcatc tcttcagtac aatgccagtc tttgtgttgc gcgttctgtg
    10501 ccagacactg tttttccagt gcttttaagc cttcgtggtc gttcgcacgc agcaattcag
    10561 tacgctcttc catcactttg taaggtgccc atgacttcgg ataaacgatg tctgcgtctt
    10621 tgaaggcttc ttccatgctg gtgacctgac ggaagctacc accggaggct ttagcattgt
    10681 ttttcgccac ttcaaccaca tccgggatca ggtcgtagcc ttccggatgg gccaaggtga
    10741 catccatacc gaagcgagtc atcagaccga tgatgccttg tggtacagag agcggtttgc
    10801 catagcttgg agagtaggcc caggtcatgg cgattttttt acctttcagg ttttccagtg
    10861 aaccaaagtg ttcacgtaac cacgcgaggt cagccattga ctgagtcggg tggtcaatat
    10921 cgcattgcag gttcactaaa gccggacgct gtggcagtac accctgcttg taaccgtcat
    10981 caagtgcagc gccaacttca cgcatatagg cgttgcctgc gcccagatac atatcgtcgc
    11041 gaataccaat agcgtcggcg cagaaggaga tcatattggc ggtttcacgc acggtttcgc
    11101 cgtgagcgat ttgtgatttg ccttcgtcga gatcttgttg tgcaaggccg agcaggttaa
    11161 gcgcggaagc ataagagaag cgggtacggg tggagttgtc gcggaatacg gaaataccta
    11221 atccactatt aaagactttg gttgagatgt tttcagcacg cagtgctttt aatgctgcgg
    11281 caacgtccag tacttgtttc agttcatctg gcgtctgttc ccacgttaac aaaaaatctt
    11341 tctcgtgaag gtgagaggtc agcgaattga tatccttaat cagctcatta acagttttca
    11401 tcatacagtc ccggtagtgg aatgacaggc aaagtggttt atttcactgt gacttgccaa
    11461 agtgattggt gttttcattg ctatacctgg gactaacaat aagtgtgcca gaattatccg
    11521 cgcggctgca aaagactaat ttgacggata atgtgtgggg ttgttcacaa attgatgcca
    11581 gcttctggtt gtttgtaaaa tacgaaaacg tttggctttt tttatcgcgt tatttatttt
    11641 ctgctaagta tcagaaataa gataaagggg caggctatct ctcttcagtt tgataaactc
    11701 accgccgtaa ttctcatatt gatatagaaa tgtgatatct cttccataat tgcattagaa
    11761 aatgataaaa atcttatgtt tttatcaaag ccaatttcgc ctgagttaat atcatcatga
    11821 tctgtgaacc atcaacgtct tcaatgatcg acttttaagt aagtctggaa ggtaaagcta
    11881 tggagcttgc tactacgcag tcagtattga tgcaaattca accgacaatt cagcgttttg
    11941 ccagaatgct tgccagcgtt ttgcagtttg aggttgagat cgttgatgaa aacttgtgtc
    12001 gcgtcgccgg aacgggcgcg tatgggaagt ttcttggtcg ccagttgagc ggcaactcac
    12061 gcctgctccg ccacgtcctg gaaacgaaaa ctgaaaaagt tgtgacacag tctcgcttcg
    12121 atcccctttg cgaaggttgc gatagtaaag aaaattgccg cgaaaaagca tttctgggta
    12181 cgcctgtcat tttacaggat cgttgtgttg gggtgataag tttgattgcc gttacccacg
    12241 agcaacaaga gcatatcagt gataatttac gcgaattttc tgattatgtt cgccatatat
    12301 ccaccatttt tgtttcgaaa cttctggagg atcaggggcc aggagataac atcagtaaaa
    12361 tattcgcgac catgatcgat aatatggatc agggcgtatt agttgttgat gatgaaagtc
    12421 gggttcagtt tgttaatcag actgccttaa aaacacttgg tgttgtacaa aataatatta
    12481 ttgggaaacc tatccgtttc agaccattaa catttgagag taattttact catggacata
    12541 tgcagcatat tgtttcgtgg gacgataaaa gtgaattaat cattggtcaa ttgcataaca
    12601 ttcagggccg acaattattt ttaatggcat ttcaccaatc gcataccagt ttttctgtag
    12661 caaatgcacc tgatgaacca catattgaac aattggttgg cgagtgccgt gttatgcggc
    12721 aattaaaacg actcattagc cgtattgcac ccagcccatc cagcgttatg gtggttggtg
    12781 aaagcggcac gggtaaagaa gtcgtcgccc gagcaatcca taagttgagc ggaagacgga
    12841 ataaaccctt tattgctatc aactgtgccg cgattccgga gcagcttctg gaaagcgaac
    12901 tgttcggtta tgttaaaggc gcatttactg gtgcttctgc caacggtaaa acagggttga
    12961 ttcaggcggc gaatacgggc acgctgtttc tcgatgaaat aggtgatatg ccattaatgt
    13021 tgcaggctaa attactgcgc gctattgagg cgcgtgaaat tctgccgatt ggtgccagta
    13081 gcccaataca agtcgacatt cgcatcattt ctgcaactaa tcagaatttg gcccagttca
    13141 ttgccgaagg taaattccgc gaagatctct tctaccgact taatgttatc ccgataactc
    13201 tgccaccgct gcgtgaacgt caggaagata ttgaactatt ggtgcattac tttttacatc
    13261 tgcatacccg tcgtctggga tcggtttatc ctggcattgc tcccgatgtc gtcgaaatat
    13321 tgcgtaagca tcgttggccc ggaaacctgc gcgagttaag caatttgatg gaatatctgg
    13381 ttaacgtggt tccttcaggt gaagttatcg acagcacgct attgccgcca aatctgctga
    13441 ataatggcac aacggagcaa agtgatgtaa cagaggtcag tgaggcgcac ctgtcactcg
    13501 atgatgcggg cggcacggcg ctggaggaga tggaaaagca aatgatccgc gaggcgcttt
    13561 cacgtcataa cagcaagaag caagttgctg atgaactggg catcggcatt gctacgctct
    13621 atcgcaagat taagaaatat gagttgttaa acacataacc tcagtgaatc cggcctgaat
    13681 tcaggccgga tatcctttta cttcgttttc tcgcaatcca gaactgtatt tacaatcatc
    13741 tgataccccg tgcagcgaca aagatttccc gccagtccgc gacgaatttc cgtaatggtt
    13801 aatggcttct cgcgtggttt cgccagcatt gccgtggtag ccataatcag gccaggcgta
    13861 caaaacccgc actgcactgc gccggatttc gcataagcct gctgaacatg agaaagtttt
    13921 ccgcctttcg cttcaccttc cagcgtgcgg atctcctttc cttcagccca ggcggcaagg
    13981 tataagcaac tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc
    14041 acgcagcacc cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc
    14101 gtgcctggtg cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc gatggtaatt
    14161 gtttcgctgt gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt
    14221 ttttggtcat cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac
    14281 gcggggcgac atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg
    14341 gcgcattttg tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac
    14401 caaatgccag gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc
    14461 caatcgttga aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt
    14521 gttctttcgg ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca
    14581 ctttgcccgg cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt
    14641 ggatctccag tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac
    14701 cgttgcaaat atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatggacg
    14761 tggccgcagc acataacgcc gggagatgac gttgagttat aggagcttct attagctggg
    14821 taaatgtcgt tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca
    14881 gctccgccag attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct
    14941 gaatcagtac gtcagtgcca tcggcgagca gtttggcctg cgggttgtca gccagcaggt
    15001 tgatggcatc ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat
    15061 cctcaaatca atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt
    15121 gtattgattg caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga
    15181 attattggtg gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg
    15241 acgaacgcgc tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga
    15301 ttggggttac ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag
    15361 ccaatgccca ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata
    15421 tgccctgaat catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg
    15481 accgtcagat caacaaaggt acagccaaac gccgatgggt tagtggtggt tttgatggag
    15541 ctttcagcag agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct
    15601 tttaacgaca ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg
    15661 gtcagattca tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt
    15721 aataatagtg ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat
    15781 gcgccgggag cgaacggcgt aacgtcggta tcttgagttg aaataacgcg aacgtcgctg
    15841 accggaaccc ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg
    15901 atttccgtcg cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg
    15961 cctgctattt ctacgccgac aggccaggtg ttagaggtat agctaaaaca ggcgacgcca
    16021 acgccgcggc gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca
    16081 aagattttcc ggcctttttc aagacactcc ggcaaccctg cgctgtaaat acgtttgccc
    16141 gtgagcggat tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca
    16201 atacctaacg ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc
    16261 gcgccataac cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg
    16321 taagcgtagg cacaacgagg ataaaggtaa gcgactttat tcctccccgc agaagcgatg
    16381 gagtgcccgt gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc
    16441 aatgttccgt cgcggttcac gcccatttgc ccgtcaatgg taaaagcgtg gcgggtacgg
    16501 gttgcgagga aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg
    16561 ctggtcagga atgctgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca
    16621 ccgacaaacg gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc
    16681 acgcggcgaa caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc
    16741 tccatccacg ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggggtctga
    16801 tagtgcccct gtacctggta gtcggcggca tcgattgttt gttggacatt acccgtcgac
    16861 atcgtgcttt gtttcagtaa attgccaccg ttatggattg gtgcagcgtc ttctgccagc
    16921 gccgcttctg gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc
    16981 gctttttctg ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga
    17041 cgaacatggc gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc
    17101 caggcatgcc cagctgtagc gaatggaata tcaggcacat cttcccaggt aaaaatcgcc
    17161 agtacgcccg gcaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg
    17221 atagggctac gtacatattt cgcataacac atgcccgcca taacataatc gtcagtatat
    17281 cgtgcccgcc cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg
    17341 gttgcttccc gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa
    17401 gatttagtct aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggtag
    17461 gaagtgtgat gacgttaaaa aaacacagat atgatacgaa acaggactct gaataggggc
    17521 agtgatacgt atcaatatga gtggcgataa aatcaatgtg ataaattggg attattacgc
    17581 atcaggatgg gaaatagggt aaaaagtcag gaagttcgat acctctacag cgaatatttt
    17641 gctgatgata cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc
    17701 cagaagcaag agattgggta tcaagaggct ggctgctatg ataaggcgtc ttgtttttta
    17761 agcgaggaaa gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt
    17821 tgttatcggt tggactgctt ttggcgggct gttcgggtag caaatcatcc gatacaggaa
    17881 cgtattccgg ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca
    17941 ccacgggaac cagcgtaaaa gaactggcgc gactgaacgg catttccccc ccttacacca
    18001 ttgaagttgg tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat
    18061 caaccgccaa atcaacgacc aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat
    18121 catcctggcc gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca
    18181 tgccgtattc gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta
    18241 cacctattta cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct
    18301 acggtaatct catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg
    18361 acacgatgct ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg
    18421 ggagcacgga tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa
    18481 ttgatccgct acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta
    18541 atcagcagtc agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc
    18601 atctcgaagc gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt
    18661 tcgattccct tcgccgcttt tgctgtatct gattttctat attatcttaa ttcaatcgct
    18721 tcagactttt cgcaatcgcc gtaatatttc cccattttgt tgtaaataaa aagcgtttac
    18781 gcaatgcaat ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa
    18841 gtggttattt acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca
    18901 ttaactcttc tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa
    18961 gtgacgagtg aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt
    19021 tatttatcgt taaatccaga taagtaccct aattatcgtc ttctgattca ctcagccaaa
    19081 ttaaaaaatg aaataaagag ccactatacc aaagacgaaa ttcaagggtt gttaacatta
    19141 acagaaaaca cacgaaagtt aacgttgacg gagaaaccct ggggaacatt tattcttgct
    19201 tctactttcg aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg
    19261 gatagtttat ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa
    19321 aaggttctgc tcaccctgtg ggattatatg tctacgctcg accagattaa gcggatgcag
    19381 gacgtcatta gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgctgtgcat
    19441 atccgatttg acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag
    19501 ctgtggaacc ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca
    19561 atcgacactg gtacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt
    19621 gcgctgctga ttgcttatct ggataaagcc aatttctatg ttatggaaga ttccggagca
    19681 tgggaagaag atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa
    19741 cgtttgtcga acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa
    19801 gctaaagcca atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat
    19861 aaaggttatg aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg
    19921 gaaaaagata aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca
    19981 aatcttgcaa agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta
    20041 gcaggtccgt atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg
    20101 ttcaatgata ttaaaaccga taccgatcaa aattcacatg cgaaaaga
//
LOCUS       DAZSBF010000060        20141 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZSBF010000060 DAZSBF010000000
VERSION     DAZSBF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.061"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..630
                     /locus_tag="ACHXWD_001707"
     CDS             <1..630
                     /locus_tag="ACHXWD_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR7620643.1"
                     /translation="LGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLCMYGLSK
                     DINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAGLLFNVE
                     PGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNKLLELAG
                     KCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTRFFH"
     gene            complement(695..1406)
                     /locus_tag="ACHXWD_001708"
                     /pseudo
     CDS             complement(695..1406)
                     /locus_tag="ACHXWD_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(1406..2647)
                     /gene="clsB"
                     /locus_tag="ACHXWD_001709"
     CDS             complement(1406..2647)
                     /gene="clsB"
                     /locus_tag="ACHXWD_001709"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR7620644.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2644..3405)
                     /locus_tag="ACHXWD_001710"
     CDS             complement(2644..3405)
                     /locus_tag="ACHXWD_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR7620645.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3538..3948
                     /locus_tag="ACHXWD_001711"
     CDS             3538..3948
                     /locus_tag="ACHXWD_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR7620646.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3910..5016)
                     /locus_tag="ACHXWD_001712"
     CDS             complement(3910..5016)
                     /locus_tag="ACHXWD_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7620647.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5027..6160)
                     /locus_tag="ACHXWD_001713"
     CDS             complement(5027..6160)
                     /locus_tag="ACHXWD_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7620648.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6153..7889)
                     /locus_tag="ACHXWD_001714"
     CDS             complement(6153..7889)
                     /locus_tag="ACHXWD_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR7620649.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7882..8877)
                     /gene="hlyD"
                     /locus_tag="ACHXWD_001715"
     CDS             complement(7882..8877)
                     /gene="hlyD"
                     /locus_tag="ACHXWD_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR7620650.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8880..9551)
                     /gene="cecR"
                     /locus_tag="ACHXWD_001716"
     CDS             complement(8880..9551)
                     /gene="cecR"
                     /locus_tag="ACHXWD_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR7620651.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9780..11144
                     /gene="rhlE"
                     /locus_tag="ACHXWD_001717"
     CDS             9780..11144
                     /gene="rhlE"
                     /locus_tag="ACHXWD_001717"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR7620652.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11376..11858)
                     /gene="ybiA"
                     /locus_tag="ACHXWD_001718"
     CDS             complement(11376..11858)
                     /gene="ybiA"
                     /locus_tag="ACHXWD_001718"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR7620653.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11978..14128
                     /gene="dinG"
                     /locus_tag="ACHXWD_001719"
     CDS             11978..14128
                     /gene="dinG"
                     /locus_tag="ACHXWD_001719"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR7620654.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14156..15118
                     /gene="ybiB"
                     /locus_tag="ACHXWD_001720"
     CDS             14156..15118
                     /gene="ybiB"
                     /locus_tag="ACHXWD_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR7620655.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            15259..16344
                     /gene="hcxB"
                     /locus_tag="ACHXWD_001721"
     CDS             15259..16344
                     /gene="hcxB"
                     /locus_tag="ACHXWD_001721"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR7620656.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16411..16470
                     /gene="ybiE"
                     /locus_tag="ACHXWD_001722"
     CDS             16411..16470
                     /gene="ybiE"
                     /locus_tag="ACHXWD_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR7620657.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16573..16833)
                     /gene="ybiJ"
                     /locus_tag="ACHXWD_001723"
     CDS             complement(16573..16833)
                     /gene="ybiJ"
                     /locus_tag="ACHXWD_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR7620658.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17098..17364)
                     /gene="ybiI"
                     /locus_tag="ACHXWD_001724"
     CDS             complement(17098..17364)
                     /gene="ybiI"
                     /locus_tag="ACHXWD_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR7620659.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17438..18115)
                     /gene="ybiX"
                     /locus_tag="ACHXWD_001725"
     CDS             complement(17438..18115)
                     /gene="ybiX"
                     /locus_tag="ACHXWD_001725"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR7620660.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18157..20141)
                     /locus_tag="ACHXWD_001726"
                     /pseudo
     CDS             complement(18157..20141)
                     /locus_tag="ACHXWD_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4642 a         5291 c         5468 g         4740 t
ORIGIN      
        1 ttaggtttgg tggcaaccgc aggactagcc tggggagcta agtatctcga aattacggca
       61 accaaatatg attcaccacc aatgtatgtc gctatagggt tattattact ttgtatgtat
      121 ggcttaagta aggatatcaa caagataaat gccgccatcg cgggcgtaat atatctgttt
      181 ttactctctt tggtggcgat tgtcgttgca agtttagttc ctgtatatgc cattatcatc
      241 gtgttcagca ctgcgggcgc gatgttttta atcagtatgc tggccggttt attatttaat
      301 gttgagcctg gttctcaccg ttttatcatt atgatgacgt tgacagggtt ggccctggta
      361 atcatcgtga atgcggcatt aatgagtgaa cggcccattt gggtaataag ttgcttaatg
      421 attgtgttat ggccaggcat tatctcgcat ggacgaaata agctccttga actggcagga
      481 aaatgccata gtgaagagtt gtggagtccg gttcgttgcg cttttacagg tgcattaaca
      541 ctctattact attttatcgg cttctttggg atacttgccg cgatagctat aacgcttgtc
      601 tggcaaaggc atacgcgttt ttttcattag ccgtaaatgc acatactaaa gaacttaact
      661 aaacttcaca tcgccgcttc atttttcgcc cgtagcccag tacaaagaaa tagttcacgc
      721 tttgaccaag taacagccag ataatcgccc ccattaccat ccagttaacg ctggaaatca
      781 gcatctgagc gagggcgaat ttccatgaag gcagcaccag cttttgccct ttgatggtca
      841 tatggcggtg cttcgcgaaa gcgcaaaacc acaaataaac cgcgataatc atcagtaagc
      901 caatgccgag aatacgcagc gtagtttgat cgacatacca gtgatccggc aactccacca
      961 cgcctgcggt aaagataatc cctgccagta aaatatagcc cagccagttg gtggtaatac
     1021 tgagcgagaa aatccgcgta atagtgctac cgggcaaccc cagacgagag tacaaacgat
     1081 agcgcatacc aatgccgccg acccaggtac tgagcgtcag gttgaaggcg tagcagataa
     1141 acgacaccag catcacctgg cgcttcgcca gtttgtgacc gcagtaaaag cgggcgagca
     1201 ggtcatagca gccgtaaatc agatagctga cgaccaccag cgaccgcact aagcagcgca
     1261 acgcgattgt agtcgcggat gaccttccag acctcttccc agtccacttt tttggcgtag
     1321 accaccagca acacaatcac cgcgataaaa aacagccagg tgaggatctt ctttgctaag
     1381 cgccagcgcg ggtgtgattt actcatcagg gttttacccc cgtgttttca gtttctaccc
     1441 gatcctgcgt ttccattgtc ggttgtgcgg gcggatcaac ctgcgccaga cgtggcgtgt
     1501 gtgccggaag ccagccaacc agcgccggga agtggcgtaa aaagtggaac gccagcacgc
     1561 ttttggtcag gttccaccag gtgcgtttgg gcagcatggt ttcatccacc tgctgacaat
     1621 ctgcggcgat aatgccgttc agattatcgc gcagcgtctg gttaaaatta cgatcgtgga
     1681 tgatgacatt tgcttcgaga ttcagtgaca aactgagcgg atcgagatta ctggacccta
     1741 ctgtcgccca gtgatcgtcc atcaatgcta ctttgccgtg gagcgggcgg cggcggtact
     1801 caaacacctg aacgccgcct ttaaccagat agttatacag caagcgcgcg ccgactctga
     1861 caatcggcat atccggttcg ccctgaatga tcagtttgat ccgcaccccg cggcgtgccg
     1921 ctttacgcaa ggcgtgtaaa aatcgatagc cggggaagaa gtaggcgttg gcgataatga
     1981 cttcccgccg cgcctgagtg agcattttca gatagtggcg ttctatatca tcgcggtgtt
     2041 cttcgttatc gcgccagacc agcaatacct gcgcgtctcc cggctggcgg ttctcttccg
     2101 ctttgtgatg acgtcgccac cagcgtcgtg ccgcgctctg tccaggcagg ttttccagct
     2161 caaactggag aatatcttcg acaatcggcc cttcaaggcg taccgcgtaa tcctgtttag
     2221 cctctggacc gtagctggac atatgctcgg cggagtaatt cagcccgcca ataaaggcga
     2281 tacgcgcgtc gatcaccaca attttgcgat gcatccggcg aaacacattg gtgcgcatac
     2341 caaaaaggcg agggcgggga tcgtagtagc ggaacactac gccagctgcc gtcagttcat
     2401 tgacaaactc atcgctgaga tccggcgaac cgtagccatc cagcaagact tccgctttaa
     2461 ccccgcgttg cgctgctgcc agtagtgccg catgcagctg tttgccgacg ttatcctcaa
     2521 accagataaa cgtttcaaga atgatgcgtt cttgtgcctc gccaatcgcc ttaaacaccg
     2581 cgggataata ttgctcgccg ttttccagca actggatctt attgccttcg cgccagctac
     2641 atttcataaa tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg
     2701 cagcggcaac gcggttggcg cgctggcgct ggcatttttg acgtagatcc tgtccagtcg
     2761 tagtagagga aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggcgc gggtaaaaat
     2821 ctcatccagt ccggcctgca cttttaacgg atgattagct ttttgccgcc agtcattgaa
     2881 atcacccgcc accaataccg gttcgccgtc cggtagctca ttcacccatt cggcgagcat
     2941 cgcaagctgc gcctgacggt gcgcctcacg caggcccaga tgtacgcaca tcacatgaat
     3001 cgcttttccg gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct tttccgcacc
     3061 atcgaccgaa acatcgcgat tctcataatg ttcaatggga taacgcgaca gtacggcgtt
     3121 gccgtgatgc ccttccgggt atacggcatt gcgaccgtag gcaaaatcgc tccacatagt
     3181 gtcggcgaga aactcgtagt gcgaggtatc gggccagttt tccacatgca gcggatgaac
     3241 ttcgtgcgcg cccatcactt cctgcaggca aacaatatcg gcgctgacgg tacgcacggc
     3301 atcgcgaagt tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt tgtgaatgtt
     3361 gatggtgagc accttgaacg aaaattgttg tgtttgatcg ggcataattt tcctgtcttt
     3421 gcctcttatc tcattgaaat agtgtagtcg gcgtcacaaa aaggtgcggt cttacggaat
     3481 tttccgtaaa gttcggtact ctgagtaagt agagataaat tcttcaggag agaagccatg
     3541 aagtggcaac aacgtgttcg tgtcgcaacg ggtctaagtt gctggcagat tatgttgcat
     3601 ttactggtag tggcgctgct ggtggtgggc tggatgagta agactctggt tcacgtcggc
     3661 gtgggattat gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt tcagcgccac
     3721 cccgagcaac gctggcgtga ggtggcagac gtgctggaag agctgaccac gacctggtat
     3781 tttggcgcag cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa caactttttg
     3841 ctggcaattg cagggctggc gatccttgct ggcccggcgg tagtgtcttt gctggcgaaa
     3901 gataagaagt tacatcacct tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc
     3961 cgttatcacc agtagcggcc acaaactatt ccacacaata tccagactcg catccttcaa
     4021 ataaatctgc ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg tcaggttttg
     4081 cagccatact ggcatgtttt ccaccggaga aacgtaaccg gaaaggagaa tggcgggcat
     4141 cataaaaaca aacacgccga taaacgcctg ctgttgtgtc gagcagagtg atgaaatcaa
     4201 cagaccgaat cccaccagcg ataaaccgta aatcaccatc gtaaagtaga acagcgccag
     4261 cgatccggcg aaggggattt gatacgccca gataccaatc gccagcacaa tggtggcctg
     4321 gaaggtggcg acaattaacg ccggtacggc tttgccgatg aagatctgcc aggtggtgag
     4381 cggcgaaacc agtagctgat cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga
     4441 agtgacgatc attacgccga tagtggtgat catggcgatc agcgacggca ccacaaacca
     4501 tttgtagtca agattcgggt tataccagtt gcgtaccacc agctcgctgt tgttaggttt
     4561 cggttttcct tccagcagct cctgctgata atttttgacg atctgttgca ggtagttggc
     4621 ggcaatttgc gcactgttgg agttacgccc gtcgaggatc aactgcaacg gcgcggtctg
     4681 gaaggtatcc agtttgcgcg agaagtcagc cgggaaacgc accagtagta acgccttttg
     4741 tgtgtcgatg gttgggcgga tctcctgtgg gcttttcagc agcagcacat gggtaaaggc
     4801 gctggcgcgg gcaaaacgtt gggtcagctc caccgaatgc tcgccgttat cttcatcgta
     4861 gatggcgatg gtggcgttag tcacttccag cgtggcggcg aacgggaaca ggatcacctg
     4921 aataagcaca ggtaaaatca gaatcgcgcg ggtttgtggt tcgcgcagca acgactgcaa
     4981 ctctttgcgg attaacgtcc ataagcgatg aaacatgctc ttctccctaa tccagccgac
     5041 gtttggtttt cagccacgtc aggccgataa acatcaccgc cgaagcgatc aaaaacagca
     5101 cgtttaccac cagcaccact ggaatattcc cggcgaggaa caggctttgc agggtgctga
     5161 cgaaataacg agcgggaata atgtacgtca ccgcgcggat caccgcgggc atactgtcga
     5221 tctgaaaaat aaagccggaa agcataatcg acggcaggaa cgcggcgttc agggcgacct
     5281 gagcggcatt gaactggttg cgggtaatcg tggaaatcag cagccccatc cccagggtac
     5341 tgagtaaaaa caggctggag ataaaaaaca gaataagcag cgaaccgcga tacggcacgc
     5401 cgagaataaa cactgacacc agcatacaca gcagcatcgc cagcatcccg agaaagtaat
     5461 aagggatcag cttacacagc agcagttccg tgcgggtaat ctccgtagag agcagagcct
     5521 ccatggtgcc gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga
     5581 ccgtcatgat gatggtcact gcaccgggga taatgaagtg ctggctaatc gccgccgggt
     5641 taaaccagta gcgggtttgt acatcaataa gcggttcaaa agtctgcccg ttgtcctccg
     5701 ctcgctgcat ttgccagatc tgccagatcc cttcgacata cccctgtaca aagttagcgg
     5761 tattcggctc actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca
     5821 tctgttccgc aaaatccacc ggaataacca ccagaccgcg aattttcccc gcctgcattt
     5881 tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc gaaccggtca
     5941 tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc cagcaagatc ccgacccgca
     6001 gcttgctgga gtcgaggtta atgccataac caaaaataaa cagcagtagc agtgggatca
     6061 ctaccgcaat cagccagcta ctcggatcgc gaacgatctg ccgcgtctct ttgacgcaca
     6121 gcgcccgcac gcgacgccag gaaaggatcg ggttactcat tgctatgctc cttatcccag
     6181 tcgtggatca actgaataaa ggcttgctcc atggtgggat cgggttgctc atcgttagcc
     6241 gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta atttcccacg gtacaccagg
     6301 ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg tggtgaccat caccgtgacg
     6361 cctttctcta ccatgctgtt gatatgtagc caaaattcac ggcgggtgag gggatcaacg
     6421 ccggacgtcg gttcgtcgag aaacagaatg tccggttcat gcatcagcga acaggccagc
     6481 gccagccgct gtttaaaacc taatggcagt tcatcggtgg cgtgggaggc gatacttttc
     6541 aggccgaacg cctcgctcat gcgggagatt ttttcgttct gcgcccgacc gcgtaagcca
     6601 tacacaccag agaaaaagcg taaattctgt tcgaccgtca ggttaccgta gagcgaaaac
     6661 ttttgcgcca tatagccgag atgctggcgc gctttaccag aactctcttt cagatccatc
     6721 cccagtacca gcgcctgacc agaggtcggc accagcaaac cgcacatcat cttaaaggtg
     6781 gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa tctccccgtg tttaacggca
     6841 aagttgacgt gatcggtggc ggcaaaatca ccaaatttct tggtcagttc tttcgcttcg
     6901 atcaccgtct cgccgggtgt gccttctacc gtatgtaata ttgcgcccag cggcgattcc
     6961 gaggttccgg caccgcccag caaatcaata aacgcatctt caaaacgcgg cgtagtttcg
     7021 ttgatgttga tttccggcat cccgtcggca tggcgaatat cgtctggtgt ggtctctttt
     7081 ttgaggatca gacgtaccga tttcccctga atcatgccgt cgctgacctg cggcagtttc
     7141 aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac ttgtcatcag aaagctgcgt
     7201 ccggccatgg tttgtgtcag ggcttttggt tccccctgat acagcaactc gccttcattc
     7261 atcagtaaca cgtcacggca ctgctcggct tcgtcgagat acgaggtact ccagaggatt
     7321 aacatccctt cgcctgccag ctcatgcacc atctgccaca gttcgcgccg tgagataggg
     7381 tcaacgccga cgccgggttc atcgagcagc aacactttcg gttcgcccac cagggtacag
     7441 gccaggccga gtttttgttt catcccaccg gagagcttgc ccgccaggcg tccggtaaac
     7501 ggcccaagag acgtaaactc cagcaggcga gcaaaagttt gcttacgtgc ctcgccggtg
     7561 acgctgcgca aatccgcgta cagattgagg ttctccatca ccgtgagatc ttcatacaga
     7621 ccaaatttct gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt tttgattgga
     7681 tcaaagccaa tcaccgtggc attgccgctg tcgggtttca gtaatcccgc cagcatccgc
     7741 attagcgtgg ttttacctgc accatccggc cccaccagcc ccgtcacata accggcgtga
     7801 atggtacaat cgagcggcgc gacggcgggc ttgtccatgc ccggaaagcg tttttccagg
     7861 ccgttcagcg tgataacggc atcattcatg tcctgcctcg ttaccgaatt gcaccgtcac
     7921 cggcattccc tggcgtaacg catcatcggc gtcggtcacc acaatacgca ggcgatagac
     7981 gaggtcggta cgcagatccg gtgtttcgac ggttttcggg gtaaattcag cagtcggcga
     8041 aacgaaacca atctgcccgt gatacggctt gtccgggcga ccatcggtat aaagcagcac
     8101 tttgcgcccc ggctgggcct ggtcaagata ccgttcatca acataagcgc gcacccacac
     8161 cggacgcgtt agtgaaacgg taaacaccgt gccaccttca ttgaggaccg tgcctggctc
     8221 caccgcgcgc gttaacagcg tgccatcaga cggggctatc aacgttgagt cctgtaaatt
     8281 caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg ctggctttcg cctgggcgat
     8341 gtcctgttca cggttaccgg aacggtactg acgcaattta tcctgtgctg atttcagcgt
     8401 tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg tcatttgccg aaatagtgcg
     8461 gcttttccac agtccttgct ggcggttata gaagttctgc gcatagtcat aggcggcttg
     8521 cgcctgtttc accgctgcgg cggcctgagc gatttcttca tcgcgatacc cggcaagcat
     8581 cagatcatac tgcgcctgtg ccaccgagac gcccgctttc gcctgcatca gggcaatctc
     8641 atacggcttg tgatccagtt cgcccagcac ctggcccgct ttgatagcat caccttcgtc
     8701 caccgccagc gattcaacgc gccccccaac acggaaacta agattcaccg tacgaatatc
     8761 cacgttgcca tacagcgtca ggccgttatc ctggcggctt tgataccacc agtagcctcc
     8821 ggcaaccacg gcggcaagta ccactaccgc cagttcgatc acgacaggtt ttttcatcac
     8881 tacaaactcc tttgcgataa tccttgcaaa atcaggtcga tatgacaggt caccgtctgg
     8941 ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa cagaattgtt
     9001 tctttgccaa gacggaacgc cagaatctca ccaatcaacg catgggtatg aaggatcatg
     9061 cgggtgtcat tggcgtcgct gccggtccag gcggcaatca ggcgtgtcag gtggctgtgt
     9121 agcggactaa tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga gagctgctca
     9181 cgggagataa acttgctgag gttgacggtg tcatcctggg tgagcagctt aatcatgttc
     9241 ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat caggctgtgg ttgggcgaac
     9301 aagcgttcgg cttcctcggc atgcggacgg aactgctcgc caataaaatc ggcaatccac
     9361 tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt agtaggtgat ggcagcgata
     9421 ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt tcattccata ttcaccaaac
     9481 tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt cacccttgat tgtcatggca
     9541 ggattattca tcgcacagtc cattcttaat caaatgattg attaagatta tgactccata
     9601 tgggagttgt ccagtatggc taagaatttt agctacgcca gtcacaggga taatttatgc
     9661 gctgcgtcac aaaaactgct acactccgct cgctcatgac attgtggttt ttgtcatttt
     9721 ccttttcagt atctccctga aaactacacc ggtaacggtc ggggcggttc ggagtagtca
     9781 tgtctttcga ttatttgggt ttaagccctg atatcctgcg cgccgttgcc gagcagggtt
     9841 accgtgaacc cacccctatt cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc
     9901 tgatggctag cgcccagacc ggcaccggca aaacagcggg ctttacgctg ccgctgttgc
     9961 aacacctgat cactcgccag ccgcacgcca aagggcgtcg tccggtacgt gcgctcattc
    10021 ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa cgtccgtgat tacagcaaat
    10081 acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag tattaacccg cagatgatga
    10141 aactgcgtgg cggcgttgat gtgctggtgg caacgccggg acgtttgctg gacctggaac
    10201 atcagaatgc agtgaagctg gatcaggttg aaatcctcgt cctcgatgaa gctgaccgca
    10261 tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt aacaaaacta cctgcgaagc
    10321 gccagaacct gttattctcc gcgaccttct ctgacgatat taaagccctg gcggaaaaac
    10381 tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa taccgcgtct gatcaggtga
    10441 cccagcacgt tcactttgtc gataagaaac gcaaacgcga attgctgtcg cacatgattg
    10501 ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac caaacacggc gctaaccatc
    10561 tggctgaaca gctcaataaa gatggcatcc gtagtgcggc gatccacggc aataaatcgc
    10621 aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg cgatattcgt gtactggtgg
    10681 caactgacat cgctgcgcgc ggcctggata ttgaagagct gccgcacgtc gtcaactatg
    10741 aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg gcgtaccggt cgtgcggctg
    10801 ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg
    10861 aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc gggctatgag ccggacccgt
    10921 caatcaaagc cgaaccgatc cagaacggtc gccagcaacg tggcggcggc ggtcgtgggc
    10981 aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga aggtggcgca aaatctgcaa
    11041 gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga tgccaaaccg gcaggcgaac
    11101 aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca gtaatctttt atgccgggct
    11161 atgcccggca tcaggctgat gaacaaacgc aaaactgcct gatgcgctac gcttatcagg
    11221 cctacgtgaa ctctgcaata tattgaattt gcatgctttt gtaggccgga taaggcgttt
    11281 acgccgcatc cggcatttca caacaagcac ttgtcagcaa tttgagaaca cgggaaaaaa
    11341 tttatctgtt ttaccgccca taagccaccg agacgttact tctctatagc caattgttct
    11401 cgcaactcca ttaaaaggta gcccagtcga ttcttgccct taccattacc accgtctccc
    11461 cagtaagcat cgttttccgt atgctcaacc agttttgcgg gcgcggttgc cagcaagagc
    11521 acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg ctttacgcat cacctgttct
    11581 ttaaccgact cccagttttt acgaagtggc ttggaacgat tacgccccat gcgagcggcg
    11641 accatggggg aagaaacccg acggatctct tcgcggtatt tttcatcaag gaatttttgt
    11701 gcctgaaaat agtgttcgga ggtgggccag gttttcccgt caaccttgat gggccaggcg
    11761 gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt agaaatttat gatggtgtct
    11821 tgcatgacgt gttggatcct ttgtgctcga acgggcatta aaccgcatta tgttggtggt
    11881 tattgcgagc cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca
    11941 caatattggc tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa
    12001 agcgcaaatt gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg
    12061 tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg ctggccggag aagaaggccg
    12121 gcatctggcg attgaagccc ccaccggcgt tgggaaaacg ctctcctatt tgatccccgg
    12181 catcgccatt gcccgcgaag agcaaaaaac gctggtggtg agtaccgcca acgtggcgtt
    12241 gcaggatcag atttacagca aagatttacc gctgctgaaa aagatcattc ccgatcttaa
    12301 attcactgcc gctttcgggc gtgggcgcta cgtttgtccg cgtaatctga cggcgctcgc
    12361 cagcactgaa cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacggcaaa
    12421 taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttatagatg
    12481 ggatggtctg cgcgatcata cggatatcgt tatagatgat gatctctggc gtcgtttgag
    12541 taccgacaaa gccagctgcc tcaaccgtaa ctgttactac tatcgtgaat gcccgttttt
    12601 tgttgctcgt cgggagattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat
    12661 ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa aatctgctgc tggtgctgga
    12721 cgaaggtcat cacctgccag atgtggcccg ggatgcgctg gagatgagcg ctgaaatcac
    12781 cgcgccctgg tatcgactac agctggactt gttcacgaaa ctggtcgcta cctgcatgga
    12841 gcagtttcgc ccgaagacca tcccaccgct ggcgatccct gaacgtttga atgcgcattg
    12901 tgaagagttg tatgagctta tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc
    12961 cgggcaggag gcagagcacc gttttgcgat gggcgaactg cctgatgaag tgctggagat
    13021 ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt tatttcttaa
    13081 cgatttaagt gagaaaaccg gcagccatga cattgtacgc ctgcatcggt tgattttgca
    13141 gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcgtctct
    13201 ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc gcgaagggca
    13261 actgcatctc tggtttcact gcgtggggat acgtgtcagc gatcagctgg aaaggctgct
    13321 gtggcgcagt attccgcaca ttattgtcac ctccgcaacc ttgcgttcgc tgaatagttt
    13381 ttcgcgtttg caggagatga gtggtctgaa agagaaagcg ggcgaccgtt ttgtggcgct
    13441 ggattccccc tttaaccact gcgaacaggg caaaattgtt attccccgga tgcgcgttga
    13501 gccttccatc gacaacgaag agcagcatat tgccgaaatg gcggcctttt tccgtaagca
    13561 ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt gccagcggac gggcgatgca
    13621 gcgctttctc gactatgtga cggatttacg tctgatgttg ctggttcagg gcgatcagcc
    13681 gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc aacggtgagc gcagcgtgct
    13741 ggtgggctta cagtcatttg ccgaagggct tgatttgaaa ggtgatctgc tcagccaggt
    13801 gcatatccac aaaatcgctt ttccgcccat cgacagcccc gtggtgatca ccgaagggga
    13861 atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa agcctgccga gcgcctcgtt
    13921 taacctgatt cagcaggttg ggcgactgat tcgaagccac ggttgctggg gcgaagtggt
    13981 tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg atgcattacc
    14041 ggtatttccg atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac
    14101 gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga
    14161 ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac cacgcgcgcg atttagaccg
    14221 ggatactgcg cgcggtctgt atgctcatat gctcaatggt gaagtccctg accttgagtt
    14281 gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg gaagcagaga tgctcggctt
    14341 ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg ccagcaggca agccgatgcc
    14401 gattgtcatc cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc
    14461 gattctcctg cacaaactcg gttttcctgt ggtggttcac ggggttagcg aagatccaac
    14521 ccgcgtgctg actgaaacca tttttgaatt gatgggtatt acgccaacgt tgcacggcgg
    14581 gcaggcgcag gcgaagctcg acgaacatca accggtgttt atgccagtcg gcgcgttttg
    14641 cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca
    14701 taccctggcg aaactggcga cgccatttgc cgaaggtgcg gcgctgcgtc tttccagcgt
    14761 ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt agcgatatcg gcgggcgggc
    14821 gctactaatg catggtactg aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat
    14881 caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa aaacaggaca ctgctggtag
    14941 cgagttactg ccacaagcaa aagatccgga aaccacggcg cagtggattg agcgttgcct
    15001 tgctggcagc gaaccgattc ccgaatcgct gaaaatccag atggtttgct gcctggtggc
    15061 tacgggtgaa gcggcaacta tcagcgacgg cctggcgcgc gttaatcagg cattttaatt
    15121 cttttctcag ccggatgagc catgtcatcc ggcttttacc ccaccgcaat atgaaattcc
    15181 tgcatcttta ttgaccttcc cacgcccggc gtgcagcata aaaatacaac aaacacataa
    15241 cataaacagg agttaaccat ggaaagtggt catcgctttg atgctcagac gctgcacagt
    15301 tttattcagg ctgtatttcg tcagatgggt agcgaggaac aagaagcgaa attagttgcc
    15361 gatcatttaa tcgcggcaaa cctggcaggg catgattcac atggtattgg catgatccca
    15421 agctatgtac gctcctggag tcaggggcac ctgcaaatta accatcatgc caaaaccgtt
    15481 aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca
    15541 catgaagcga tggcgctggg gattgagaaa gcgcatcagc acggtattgc cgccgtggcg
    15601 ctacataact cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg
    15661 gggtttgtct ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac
    15721 ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg ttttccctcg taaagataat
    15781 ttcccgctgt tgcttgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc
    15841 tggcataaag gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg
    15901 accaatccgg cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat
    15961 aaaggctacg cccttgcagc gatgtgtgaa attcttggcg gggcgctttc cggcggtaaa
    16021 acgacgcatc aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact
    16081 atcatcatca acccggaact cttcggcgcg ccggattgta acgcgcagac cgaagccttt
    16141 gccgagtggg tgaaagcctc gccacatgat gatgataagc cgattttgct accgggcgag
    16201 tgggaagtga acacgcgtcg cgaacggcag aagcagggga ttccactgga tgcgggaagc
    16261 tggcaggcca tttgtgatgc agcgcggcag attggtatgc cggaagagac gttgcaggct
    16321 ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg
    16381 gtaacatagg atcaatgagg gttagagcat atgcgtctgt cggcaaacag acagggaaat
    16441 acttgtgctg gacgtagcgt aaacgcctga tccgtccaac ggttcggatt ttgtaggcct
    16501 gataagacgc ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat
    16561 ccgtcctacg aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc
    16621 ggaagtgatg ctgtatccac tggcaccggc tgccgcggct ttctcagcca gtttcgcttc
    16681 cagggcatcg agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg
    16741 tgcctgggat gccgttatcg gttccgccgc gaacacgcca aatgacagag ttgaaagagc
    16801 catagcagca acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt
    16861 ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc
    16921 gaagagaatt gaccgccttg ttcaaataaa ttgattgata tctaacatac tgataaacca
    16981 tttatttgtt gtacttatga acttgtttta taccgcgtgg cagtcacagc agcgttaaag
    17041 gcacggaatg ccacgcaaaa taaaaattat actcgctatg catctcgcca gttgtagtta
    17101 acgtaactgg ctgtctttcg aacctctgcg attatatcct gtataagctg gtttttgtaa
    17161 atctttctcc tgctgacaat gaatacataa gcgcacgcca ggaatggctt cccgacgggc
    17221 ctgcgggatg ggggcaccgc actcttcaca ttcatccagg ctttcgccgc gcggaatttc
    17281 accccgagcg cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc
    17341 atcgttagcc caaccggatg ccatatcgac ctccccatat caatacttgt acagttaagt
    17401 gtagctaatc cagggacgaa ctcgggcagt tcaagcatca gatctccgac cattcccgca
    17461 gcagattatg ataaagatta agcagcgaca ggatctcttc actttcgccg tagcggcttt
    17521 tcagcgactg aatattgttg tccagttcaa acagcatggc gcgcttttta tcatcgcgga
    17581 tcatcgactg gatccacata aatgatgcca ctcgtacgcc gcgggttacg ggtggcacgc
    17641 aatgctggct gctggaggga tacaacacga gatcgcctgc cgggagtttt acccgatgtt
    17701 gtccgaaggt gtcattaacg accagttcgc cgccgtcgta gctttgtgga tcgcttaaaa
    17761 acagcgtggc agaaaggtca gtacgcatcc agccgttttg tggatggctg cgtactgcgc
    17821 catccacatg gaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa
    17881 gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct
    17941 cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac ctgttgattg ttcttaactt
    18001 gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca ttcggcttgt tccagttgtt
    18061 cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg aatgtggtac atcatacaag
    18121 ttctccaaaa agtggggcct gcgccccaca tctgaatcag aaatgcatat tggctgtgag
    18181 caagaaggtt cttggctcgc ccgggtgata acggtagccg ctcttgttga ttgaggcgac
    18241 gtaatcagta tcaaacaggt tgtagacgtt tagctggaag tcgagattgc gattaacccg
    18301 ataccccagt ttggcatcgg cgacccagta accttcggta aatgctggcg ttcccaccgc
    18361 gccgtctgaa cctttatgca tactgccgat atagcgtgcg cccgcgccaa cagagatatc
    18421 gtcggttgcc tgatattggc tccataaggt gaaggcgtgc tccggggtat acggcagcga
    18481 tgaggaacca tcctgggcaa catctttgcc gtttttgatg gttgcttttt gctgggtata
    18541 gccgccaatc acctgccacg cgggagtgat attcccggcc acggatatct catagccttc
    18601 gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca ttttgctcaa cttcattttc
    18661 gatatcagta cggaacagcg cggcggtgag caacagacgt ttatccagaa cctgccattt
    18721 ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa tcggtgcggt tggcactgtt
    18781 accgctgcca gactgcgcaa gggcgaagtt gttgccgccc ggaggctgct gggaaacggc
    18841 atagttaata tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt
    18901 catcagattg cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacacc
    18961 agttgggcag gtgatggcac cgcgtccgct gccgccgcag gcggtggcac tgtcatattc
    19021 agtatgataa ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag
    19081 cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg
    19141 cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa atatttaccg cgggtaacgt
    19201 caccggatta acgccgtagt tagtctgcgt ttcacgggta aattccacgc cggtactgac
    19261 atcatgaccg atagaaccgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat
    19321 tttattactc acatctttgg tattcgccgt gcgtgaccag gttcagctat tcacatcgct
    19381 ggtgggctga gtaatattcg acgccccgcc cataatcgcc gtcatcaggt aatcctgctt
    19441 tacgcgcgac caacgggtag tattgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa
    19501 acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt
    19561 atgagtatca acttttccgg aatgattcag ggccgccgtt cccgcagatg gggcagaata
    19621 gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg ttgtgctggg tgacatgcag
    19681 ataattaaga tacaaacgat tcgctgtacc aaggccaaaa gcgacagaag gggcgacgcc
    19741 gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccattacatt
    19801 caggcgcacc gcggtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa
    19861 ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgtttgct
    19921 gatcatattg atcgagcccg tcggtgcgct gcgcccgtag tcggtgccgg acggcccttt
    19981 aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat
    20041 gccatcaata taaatactgt tagaggtatc ggcaccacgc atataaatgg cgtcgccagt
    20101 ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg c
//
LOCUS       DAZSBF010000061        20047 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZSBF010000061 DAZSBF010000000
VERSION     DAZSBF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.062"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..69
                     /locus_tag="ACHXWD_001727"
     CDS             <1..69
                     /locus_tag="ACHXWD_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFR7620661.1"
                     /translation="ILKTTDYLRIAQPELLNFRRTA"
     gene            complement(70..174)
                     /gene="yoaI"
                     /locus_tag="ACHXWD_001728"
     CDS             complement(70..174)
                     /gene="yoaI"
                     /locus_tag="ACHXWD_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR7620662.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            344..790
                     /locus_tag="ACHXWD_001729"
     CDS             344..790
                     /locus_tag="ACHXWD_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR7620663.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(747..1568)
                     /gene="nimR"
                     /locus_tag="ACHXWD_001730"
     CDS             complement(747..1568)
                     /gene="nimR"
                     /locus_tag="ACHXWD_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR7620664.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            1665..2846
                     /gene="nimT"
                     /locus_tag="ACHXWD_001731"
     CDS             1665..2846
                     /gene="nimT"
                     /locus_tag="ACHXWD_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR7620665.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            2901..3248
                     /locus_tag="ACHXWD_001732"
     CDS             2901..3248
                     /locus_tag="ACHXWD_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR7620666.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="ACHXWD_001733"
     CDS             complement(3270..3524)
                     /gene="yoaF"
                     /locus_tag="ACHXWD_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR7620667.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            3707..4732
                     /gene="dgcP"
                     /locus_tag="ACHXWD_001734"
     CDS             3707..4732
                     /gene="dgcP"
                     /locus_tag="ACHXWD_001734"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR7620668.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            4765..4863
                     /locus_tag="ACHXWD_001735"
     CDS             4765..4863
                     /locus_tag="ACHXWD_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR7620669.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            4866..4940
                     /gene="yoaJ"
                     /locus_tag="ACHXWD_001736"
     CDS             4866..4940
                     /gene="yoaJ"
                     /locus_tag="ACHXWD_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR7620670.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(4999..5247)
                     /locus_tag="ACHXWD_001737"
     CDS             complement(4999..5247)
                     /locus_tag="ACHXWD_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR7620671.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(5395..5577)
                     /locus_tag="ACHXWD_001738"
     CDS             complement(5395..5577)
                     /locus_tag="ACHXWD_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR7620672.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="ACHXWD_001739"
     CDS             complement(5581..5940)
                     /gene="yeaR"
                     /locus_tag="ACHXWD_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR7620673.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="ACHXWD_001740"
     CDS             complement(6113..6751)
                     /gene="leuE"
                     /locus_tag="ACHXWD_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR7620674.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="ACHXWD_001741"
     CDS             complement(6878..7801)
                     /gene="dmlR"
                     /locus_tag="ACHXWD_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR7620675.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            7904..8989
                     /gene="dmlA"
                     /locus_tag="ACHXWD_001742"
     CDS             7904..8989
                     /gene="dmlA"
                     /locus_tag="ACHXWD_001742"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR7620676.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVAIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            9240..10850
                     /gene="yeaV"
                     /locus_tag="ACHXWD_001743"
     CDS             9240..10850
                     /gene="yeaV"
                     /locus_tag="ACHXWD_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR7620677.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGGAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            10882..12006
                     /gene="yeaW"
                     /locus_tag="ACHXWD_001744"
     CDS             10882..12006
                     /gene="yeaW"
                     /locus_tag="ACHXWD_001744"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR7620678.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12062..13027
                     /gene="yeaX"
                     /locus_tag="ACHXWD_001745"
     CDS             12062..13027
                     /gene="yeaX"
                     /locus_tag="ACHXWD_001745"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR7620679.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="ACHXWD_001746"
     CDS             complement(13081..14196)
                     /gene="rnd"
                     /locus_tag="ACHXWD_001746"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR7620680.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="ACHXWD_001747"
     CDS             complement(14278..15963)
                     /gene="fadD"
                     /locus_tag="ACHXWD_001747"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR7620681.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="ACHXWD_001748"
     CDS             complement(16168..16749)
                     /gene="yeaY"
                     /locus_tag="ACHXWD_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR7620682.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="ACHXWD_001749"
     CDS             complement(16789..17484)
                     /gene="tsaB"
                     /locus_tag="ACHXWD_001749"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR7620683.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(17542..19452)
                     /locus_tag="ACHXWD_001750"
     CDS             complement(17542..19452)
                     /locus_tag="ACHXWD_001750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR7620684.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            19584..19928
                     /locus_tag="ACHXWD_001751"
     CDS             19584..19928
                     /locus_tag="ACHXWD_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR7620685.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         4861 a         5195 c         4914 g         5077 t
ORIGIN      
        1 attttgaaaa ccacggatta tttgcgcatt gcgcaaccag aactgctcaa tttccgccgc
       61 actgcgtaac tagcctgtcc gttcaataag cagaacggac aaaaccagca caacagcaat
      121 ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg
      181 cccccgggag atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac
      241 aattcgcctg attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaaa
      301 cgtaaagtaa gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt
      361 gctgatcctg ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt
      421 ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat
      481 tgaaaaacag gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat
      541 cgccagcggg acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct
      601 ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt gggcgcggcg tgaccttaat
      661 gggcagccag ccgcaactgg tcgccgggct gctggtaggc actgttttag gcgtggcgct
      721 gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg
      781 gaaacagtag ttaatcccgc aatatatcgg cctggcgttt gccccaggcc tttcttaaac
      841 atagtaataa aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta
      901 tcgcctttca ctaatccctg aagtgccata atcagctgta actgctgacg ccactggcga
      961 aaactcaacc ccgtctcttt tactatcagt cgcgcaagat tgcgttcact catcgcaaaa
     1021 aagccagccc attgccctaa tgacccccac tcgacgggcc ctttcgccat catctccacc
     1081 atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga
     1141 gggagttcat caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc
     1201 gtcgtcctgt ttgccagggt taaaatcaat tcccggcata agggcgatat tttcaatgtg
     1261 caacatgttg tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg
     1321 ttagctgtca cttgattgct atgttccacc ccgcccggta tccaaaccgc atattgcggc
     1381 ggcaccatcc ataaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc
     1441 agctgtcctt tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata
     1501 caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg
     1561 tgcatcattt tgtccgaatt tagcgataat ttgtcatttt agcttgattc aacataacaa
     1621 taaaaacggt aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt
     1681 cattaagcgg caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat
     1741 tacgcgtcac ctttatcggc gcagcaccgt tactggatac gattcgttcc gcttactcgc
     1801 tgacgacagc gcaaaccggc ttattgacca ccctgccatt attggccttt gcgctaatct
     1861 cacctttggc caccccggta gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt
     1921 tacttttgat ctgtgctggt atcgcaattc gctctctccc ttcgccttac ttattatttg
     1981 gcggtacagc ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa
     2041 ttaaacgcga tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg
     2101 gagctgcagc ggcactggga tcggctatga tagtgccgct ggctttgaac ggttttggct
     2161 ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc
     2221 cacagtggcg aagccaacaa catgcaaatt tgagtacctc gcgcgcctta catactcggg
     2281 gtatctggcg ttcgccgctt gcctggcagg taacattgtt tcttgggatc aactcactga
     2341 tctattacgt gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag
     2401 cacaggcggg ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga
     2461 tccctctttt cttacatcat gtgaaagatc agcgtggtat tgcagcgttc gttgccttga
     2521 tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc gcacgcgatc acctggactc
     2581 tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc
     2641 gggcaagttc tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt
     2701 tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtgactggt
     2761 ccgtaccact gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg
     2821 ccgggagaga caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcatgcg
     2881 tgaaagggag aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac
     2941 agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg
     3001 atttagccct tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct
     3061 ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca atatcttgca gaactggcgc
     3121 aacacgagca agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc
     3181 tctacgcagc aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta
     3241 gcttgtgatt ttagtacagc atccggcggt tatttttcac cagccggatg gtcccgtcgc
     3301 cacaatgccc attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct
     3361 tgcgctgtcg acactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga
     3421 tttgccatac caatctgcgg cggtttaggt acttctggct gagaagaggt tgaacaacca
     3481 gccaggacca gcaagcaagg caaagcaaaa ctgataattt tcatttattg atctcactca
     3541 tttatctacg cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat
     3601 tcactcaaaa catattacgt cttgtttcat ctttgttggt gatgttttat catgcctgca
     3661 aagattaaat aatcagcatt tacccgccgt atcctggagt tgtttcgtgt cagatcagat
     3721 tatcgcccgc gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca
     3781 gcttctggag atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga
     3841 tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac agccagaaaa tgcacatccc
     3901 ggagaatttt accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg
     3961 ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg
     4021 catctccaca tttctgagca caccaattca cttaccggat ggatcattct atggcacgct
     4081 ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt
     4141 attcgccgga ctgattgcac aatatattca aaaagaggca ctggttgaac agttgcgcga
     4201 agccaatgct gcgctgattg cgcaatcgta taccgactcg ttaaccgggt tgccgaatcc
     4261 gcgggcgatt tttgaaaatc tgacgacgct gttttctctc gcccggcatc ttaaccataa
     4321 gataatgatc gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca
     4381 taatagtggc gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa
     4441 tggcgaagtt attggtcgtc tcggcggtga tgagtttttg gttgtttcac taaacaacga
     4501 gaatgcggat atttcgtcgc tgcgagaacg cattcagcag caaatacgtg gagaatatca
     4561 cttaggtgat gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc
     4621 tgaaacaacc gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa
     4681 aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg ccacattgct gaggcgtatt
     4741 cacatccttt tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttt
     4801 attacagcgg tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggggca
     4861 taaagatgaa aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca
     4921 ctgagctggg atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc
     4981 ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc
     5041 accaataacg gcaaccacga agctgccaaa attgaagcca tcgactttac caaagccaaa
     5101 cagcgtgctg atccatccgc cgactacggc accgactatc cccagcagga tagtcataaa
     5161 gaatccacca ccatctttac ctggcatgat ccacttcgcc agaataccgg caataagccc
     5221 aaaaataatc catgacagaa tgcccattgt ttcctcactt atctgttttg cattagcggg
     5281 ttagtcgctg ataaaaagca tagcacaaca tcgggagggc aagatttgtg acgagcatca
     5341 cggaggattt ttttgcgatg gcgcagaaat tgcgccatca acgatcagtg ataattacca
     5401 accacaaaca tcatgttcgt tttccgtgtc ataagaacgc acggtattca ccagatcttt
     5461 tatcacttca gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc
     5521 gacagaaacg ccattactgt tattggtgac ggtcacggta tacgttgctt tgcccatgat
     5581 tcatttcccg ttatgaatga ctttccgttg ttgcgcacct tccatcagga cttcaggagc
     5641 cacgaagaag tcaatgttga aataagtatc gtcagtcatg gcttcaatgt tgtgccactt
     5701 ttctggaggg aacacctcaa actgccccgc ttcgataagg atcacctgat caggctctgc
     5761 actgtgttca tcagcgtagc cgagatattt gaccgcccca tgcataacgg aaaggcgtgg
     5821 gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg aatattccgg caggcgcagt
     5881 ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga tttgcagcat
     5941 ccttttccct ccttcggtga atgcgctgaa aacggtttat tccagccgtt tcagggtacg
     6001 cctgataatt tgcattttaa ataccattta ttggttactt tttagcacca tatcagagaa
     6061 aaatcaggga ggattacaag tgggaagccc gcgacaacgc gggctgaatg tatcaggatt
     6121 gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt gagttgccaa
     6181 ctttagccag tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca
     6241 ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa
     6301 ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa
     6361 tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg
     6421 gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc
     6481 ccagatagag caaataaaac gcaccaagat aacgtacaat gttgaataat atcggggtgg
     6541 tcttaattaa tgtcgccact ccagcccatg ccagaaacat caatactgca tcgccaataa
     6601 atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta
     6661 caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc
     6721 agtaattcag aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat
     6781 gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg
     6841 ttttaacccg atatcaaccc gataattgaa taattaatca ggcatgctcc agtgaaaaat
     6901 tcgggtagtg ctcggcaaaa tactggcgta aaaattctat cgttatccgc actttcgctg
     6961 acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta
     7021 gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag gagcgcagag
     7081 caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg
     7141 ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag
     7201 gatgatcgcg ctctttaatg accaggcacg gcaatgcgga aagatcggtt aaatgtttcg
     7261 gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttagtcgcca
     7321 gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcaacac
     7381 cttcattgac caaatcgacc aaccggtctt ccacatcaaa acgtaattcc agttgcggat
     7441 acgccttagc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc
     7501 tgataatacg cagcatcccc tgcggcacct gacgcacgtc agaaagttcg tccatcatct
     7561 gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa
     7621 tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg
     7681 cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac
     7741 cagcccggcg agccaccagc ataaagacgc gcaaatcgtt cagcagcggt aaattattca
     7801 tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg
     7861 atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg
     7921 tattgctgcg atcccgggag atgggattgg caaagaagtc cttcctgaag ggattcgcgt
     7981 attacaggct gccgctgagc gctggggctt cgacttgagt tttgagcaaa tggagtgggc
     8041 gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact
     8101 tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggataccg ttccggacca
     8161 tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct
     8221 gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga
     8281 catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag
     8341 agtgaatgaa ggtacagagc atgaagtcgc cattcaggaa tcggtattta cgcgtcgtgg
     8401 tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct
     8461 aacttctgcc actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt
     8521 ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct
     8581 ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttgtt
     8641 tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc
     8701 cgccaacctg aatccggaac gcactttccc gtcgctcttc gagcctgtcc acggttccgc
     8761 gccggatatc tacgggaaaa atattgctaa ccctatcgcc acaatttggg ccggtgcgat
     8821 gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct
     8881 ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca ccggatatga aaggcagtgc
     8941 caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag
     9001 tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt taataacctg
     9061 cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg
     9121 tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc
     9181 tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca
     9241 tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc
     9301 ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacacaa
     9361 tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa
     9421 tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa
     9481 aaccggaata cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt
     9541 ctacgcttta ttgggggggt gctgaatggg cctattatta tcaaacgcct ggattaaata
     9601 tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg
     9661 gcatcagcac ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg
     9721 tgcgtaaaaa caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc
     9781 cgcaaggccc atggggaaaa ctggttgatt tgatgtttct gatcgccact gtcggcgcac
     9841 tgactatttc ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg
     9901 gtttacccga taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt
     9961 gcctaagctc gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct
    10021 ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg tccaaccgaa tttattacca
    10081 acagcatcat caatgccatc ggcctgacca cgcaaaactt cctgcaaatg agcttattca
    10141 ccgatccgct tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt
    10201 ggatctcata cccccctggc gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga
    10261 ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg
    10321 gcgtaatgga aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg
    10381 tgctggaaac actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg
    10441 gtaaattgtt cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg
    10501 atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa tctccaggaa ggtgacgatc
    10561 ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct
    10621 tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct
    10681 tcctcgtcat tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattatg
    10741 ccgacattcc ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg
    10801 aaaaaacacc agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat
    10861 cctaacgata aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat
    10921 ttttgcgcta acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac
    10981 gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc
    11041 agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg
    11101 ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt
    11161 catcagttgt tgagcggtga aggaaaagca aaaaatgtga ttacctgccc atatcacgca
    11221 tgggcattca aactcgatgg caatctggcc catgcacgta actgcgaaaa cgtcgccaat
    11281 ttcgatagcg acaaagcgca actggttccg gtgcgtctgg aggaatatgc cggattcgtc
    11341 ttcatcaaca tggaccccaa cgccaccagc gtagaagatc agttacccgg tctgggcgcg
    11401 aaagtgctgg aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc
    11461 cgcacgcctg ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt
    11521 ccggcgcatc caggtttctc cgactccgta caggttgatc gctactggca caccatgcac
    11581 ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa
    11641 gagggtacgg atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc
    11701 accccgatca aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc
    11761 ctgcaaaact acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg
    11821 attgagtggt atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag
    11881 aaagggctga aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt
    11941 agtggcattt ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag
    12001 gactaatgac atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc
    12061 aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca
    12121 ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg
    12181 aagtcacatc attgtgcaga tgagcgatgg tgataaccag tacagcaatg cgtattcact
    12241 actgagttcg ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc
    12301 gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc
    12361 aacgcctaat aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg
    12421 cggtattggt atcacccctt tcctgtcgca catggcagag ttgcaacaca gcgacgtcga
    12481 ctggcagcta cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt
    12541 ccagcatccg caggctgaga aagtccattt gcatcattcg tcaaccggaa cacgactgga
    12601 attagcgcga ttattggcgg atatcgaacc tggcacacac gtttatacct gtggccccga
    12661 ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgccg ccgatacgct
    12721 gcactttgag caatttgcta tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc
    12781 ccgttccgga aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa
    12841 taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac
    12901 agcaatactg gaaggtgaag ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc
    12961 cagccagcaa agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga
    13021 tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt
    13081 ttactgcgga tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca
    13141 gccggaaatc agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag
    13201 ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac
    13261 gtcagtaatc agcgacttaa tcgctttaaa cgctttacga taacccggca tgtccatcag
    13321 gttaagcatc ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc
    13381 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
    13441 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
    13501 gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc cagtcggcta acagttgcag
    13561 acaggccagt tggcgtgtgc gtaattgcca ggcattggtg atatcacgcc aggcatcttc
    13621 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
    13681 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
    13741 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
    13801 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
    13861 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca ggggttgtgg
    13921 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
    13981 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
    14041 atcaattagc gccagatgct caccatcgaa aagttgaatc aaccccagct gcgggtaata
    14101 agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc ggaaagacac ggacggcttc
    14161 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgtattctct
    14221 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacgctca
    14281 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
    14341 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt
    14401 gagctgacgg cagcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac
    14461 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
    14521 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc
    14581 ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat
    14641 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatcgg tagcatcggg
    14701 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
    14761 cggtggtact tcattatcgt cgtcatccac cagtttggct tccgtcgacg gcaccggcaa
    14821 accgatgcta ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca
    14881 ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc
    14941 caccacttgc tgcactggca tcccaccgcc tgcggaaaga tgcagactgg agaaatccag
    15001 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc cagtgatagc
    15061 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat
    15121 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
    15181 gtgatacagc ggcagtgccg ttactaccag ttctttacct ggatgcagca acggcccata
    15241 ggtggcgttg acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
    15301 cgccacacct gtggtgccgc cggtgtattg caggaaggct aaatcttccg gcaccagttc
    15361 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
    15421 tggcagatgg tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt
    15481 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
    15541 tttatcaacc actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc
    15601 gccgctatcg ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac
    15661 gacgatcatc ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg
    15721 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
    15781 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa
    15841 cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac atatctatca gagattgata
    15901 acggtcaggg ttgatctccg tcggaacatc cgcgggataa cggttaagcc aaaccttctt
    15961 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaagtg ataaacatgt
    16021 tgttaacata acattaactc atcttaccag cttgataatt agacaacgaa aaggttgcga
    16081 agcgcgtcac tatttatttt tatagttgcc gtatgaacgc agaaacagcg gaccagccgc
    16141 tgtttctttt tctttaaaaa caacttgtta ctcggttaca attgtttcaa ctcttgcagg
    16201 gccaggatta taccaacccc atccgccgta accatagggc cagccgcgtc caccataaaa
    16261 ccatggatca attggctgcg gcggcataat cacctgttga gtgatatgcc agcgtttgta
    16321 acctgttacc tgcataacca taaatttata tggtgcatta tttactttgc catccactgt
    16381 gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga aaatcgaccg gatcaagaaa
    16441 accgttcaca tcggcataaa tgcgcccgcg tgaaggttct cccaaggttg gtcttgctcc
    16501 gctgtccagc ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccac
    16561 aaccactttg ccgccaaagc gcgcctcctg accaacatat aaatctggcg cattcatcac
    16621 gcgtaccaga tcttgctgtg gtgttggact gctgccttta atggcgtccg gcacagtgac
    16681 acaaccgctc agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg
    16741 aaccgccatg atgcgactcc tttttttctc agggcatact cttaagattc attctttgcc
    16801 cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc
    16861 caccgtttta ccctcggcaa acatctgaca cgcaatcggc agcatatctt ccgccgctgg
    16921 cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttacggagat ccggccaggc
    16981 ttgccagccc gtccctaccg ttacccattc gccggaaagc tgttgcattc gttcatgaac
    17041 gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc cagataccgt tttcatcacg
    17101 ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt
    17161 tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt agcgtagaga caccaatcat
    17221 cggtaactcc gcgccaagcg ccagcccttg cgcaatacca ataccaatgc gcacgccagt
    17281 aaagctaccg gggccgcgcc cgtaagccag agcgtttatg tcagtcaggg aagttccgct
    17341 ggtggtcagg atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag
    17401 ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg acagagcaga cctctgtcgc
    17461 ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat
    17521 cataccatac tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg
    17581 ggcaatgtca cgggtgcgtg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg
    17641 acgcatcacc agccgattgt cacaaattac cagcacgccg cgatcgtcgg cgtcgcgaat
    17701 cagtcgccct actccctgct tgagagtaat gaccgcatcc ggcagctgca cttcatcgaa
    17761 cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct tttaacagta gatcatccgg
    17821 cgaggtaaac ggcaatttgt cgataattac caatgacaat gtatcgccac gcacgtccac
    17881 cccttcccag aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg
    17941 caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg
    18001 gaactgctcg gccagatcgc gcatcatggc atgcgaggta caaagcataa aacaacgacc
    18061 gttgttagct tcgatgatcg gtcgcagcat tgccgccagt tgccgtgcac aacccggttg
    18121 attggtttgc ggcaggttgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg
    18181 gcttggcaat agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag
    18241 atcgtcgttc accgacagcg ttgctgaggt gaagatccag ctaccgggct tttgcgccat
    18301 taactctttg aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga
    18361 agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg
    18421 tgtgcgatac aacgtggcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga
    18481 cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg
    18541 aatttgcgga ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg
    18601 cagacgaaaa tcctgtgcac tctgggcaag acgatcagcg cacttttgta actgctgggt
    18661 gtcttttaat tcggtgcggt aggcgatggt gatgtctttt gccaggtcga gcagttgtcg
    18721 actggagagt gactgaccaa aatactggct ggcaatgtcc ggtagctggt gggcttcgtc
    18781 gaagatcatg acgtccgctt cagggatcaa ctcgccaaat ccactctctt taaccaccat
    18841 atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg
    18901 tgctttgacc acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt
    18961 ggtgctggtg accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat
    19021 atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg
    19081 taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgttcgaggc agaggtagtt
    19141 tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg
    19201 caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt
    19261 ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc
    19321 ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg
    19381 ctgtggttct cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa
    19441 atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct
    19501 ttcgccagtt gaagtggtga cggcaacctc acattgtggc agtctttgca gcacgaaaat
    19561 ggaagtataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt
    19621 ctgatgtagt aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg
    19681 ccgatgcctt tgagcaaacc gccaacacgc tggcacagat tgacgccgtg ctggaaaaac
    19741 agggcagcaa taaatcgagc attctggatg ccaccatttt cctggccgat aaaaacgact
    19801 tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccagtgcgct
    19861 gcacggtaca agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg
    19921 cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg
    19981 gttttacata aatcgaccag agaataagat attactgttg atgcttctgc taattcatta
    20041 tttatat
//
LOCUS       DAZSBF010000062        20000 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZSBF010000062 DAZSBF010000000
VERSION     DAZSBF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.063"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            286..1380
                     /gene="gcvT"
                     /locus_tag="ACHXWD_001752"
     CDS             286..1380
                     /gene="gcvT"
                     /locus_tag="ACHXWD_001752"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR7620686.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1404..1793
                     /gene="gcvH"
                     /locus_tag="ACHXWD_001753"
     CDS             1404..1793
                     /gene="gcvH"
                     /locus_tag="ACHXWD_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR7620687.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1912..4785
                     /gene="gcvP"
                     /locus_tag="ACHXWD_001754"
     CDS             1912..4785
                     /gene="gcvP"
                     /locus_tag="ACHXWD_001754"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR7620688.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5051..5794
                     /locus_tag="ACHXWD_001755"
     CDS             5051..5794
                     /locus_tag="ACHXWD_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7620689.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5851..7284)
                     /gene="bglA"
                     /locus_tag="ACHXWD_001756"
     CDS             complement(5851..7284)
                     /gene="bglA"
                     /locus_tag="ACHXWD_001756"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR7620690.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7329..7640
                     /gene="yqfB"
                     /locus_tag="ACHXWD_001757"
     CDS             7329..7640
                     /gene="yqfB"
                     /locus_tag="ACHXWD_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR7620691.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7804..8463
                     /locus_tag="ACHXWD_001758"
     CDS             7804..8463
                     /locus_tag="ACHXWD_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR7620692.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8659..9639)
                     /gene="ygfZ"
                     /locus_tag="ACHXWD_001759"
     CDS             complement(8659..9639)
                     /gene="ygfZ"
                     /locus_tag="ACHXWD_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR7620693.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9882..10148
                     /gene="sdhE"
                     /locus_tag="ACHXWD_001760"
     CDS             9882..10148
                     /gene="sdhE"
                     /locus_tag="ACHXWD_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR7620694.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10129..10536
                     /locus_tag="ACHXWD_001761"
     CDS             10129..10536
                     /locus_tag="ACHXWD_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR7620695.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10576..11097)
                     /gene="fldB"
                     /locus_tag="ACHXWD_001762"
     CDS             complement(10576..11097)
                     /gene="fldB"
                     /locus_tag="ACHXWD_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR7620696.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11209..12105
                     /gene="xerD"
                     /locus_tag="ACHXWD_001763"
     CDS             11209..12105
                     /gene="xerD"
                     /locus_tag="ACHXWD_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR7620697.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12130..12840
                     /gene="dsbC"
                     /locus_tag="ACHXWD_001764"
     CDS             12130..12840
                     /gene="dsbC"
                     /locus_tag="ACHXWD_001764"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR7620698.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12846..14579
                     /gene="recJ"
                     /locus_tag="ACHXWD_001765"
     CDS             12846..14579
                     /gene="recJ"
                     /locus_tag="ACHXWD_001765"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR7620699.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14670..15768
                     /gene="prfB"
                     /locus_tag="ACHXWD_001766"
     CDS             join(14670..14744,14746..15768)
                     /gene="prfB"
                     /locus_tag="ACHXWD_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR7620700.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15778..17295
                     /gene="lysS"
                     /locus_tag="ACHXWD_001767"
     CDS             15778..17295
                     /gene="lysS"
                     /locus_tag="ACHXWD_001767"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR7620701.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17338..17886)
                     /gene="idi"
                     /locus_tag="ACHXWD_001768"
     CDS             complement(17338..17886)
                     /gene="idi"
                     /locus_tag="ACHXWD_001768"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR7620702.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17941..18012
                     /locus_tag="ACHXWD_001769"
     CDS             17941..18012
                     /locus_tag="ACHXWD_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620703.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(18009..18134)
                     /gene="yqfG"
                     /locus_tag="ACHXWD_001770"
     CDS             complement(18009..18134)
                     /gene="yqfG"
                     /locus_tag="ACHXWD_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR7620704.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18136..19584)
                     /gene="uacT"
                     /locus_tag="ACHXWD_001771"
     CDS             complement(18136..19584)
                     /gene="uacT"
                     /locus_tag="ACHXWD_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFR7620705.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         4911 a         5021 c         5353 g         4715 t
ORIGIN      
        1 ttagctctta atattgcctt attcaaatta gctttctaat ggcatcatct ttatatagaa
       61 actggtgtgt tttttggttt tttattctgt cgcgattttg gcgtttttta accataagct
      121 aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa gttcaggcaa
      181 aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggtttcg tggtgataat
      241 ttcaccatga aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
      301 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
      361 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
      421 atgtttgatg tgtcacatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
      481 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
      541 tcggggatgt tgaatgcctc tggcggtgtg atagatgacc tcatcgtcta ctactttact
      601 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
      661 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
      721 gccgtgcaag ggccgaatgc gcaggcaaaa gctgccacac tgtttaatga cgcccagcgt
      781 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg cgggcgatct gtttattgcc
      841 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
      901 gatttctggc gtgcgctggt ggaagcgggt gttaagccat gtggcttggg cgcgcgtgac
      961 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
     1021 cctttagccg ccaacatggg ctggactatc gcctgggaac cggcagatcg tgactttatc
     1081 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
     1141 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
     1201 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
     1261 gcgctggcgc gcgtgctgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
     1321 gaaatgtcgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
     1381 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
     1441 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
     1501 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
     1561 gcgcgggcga tgactgcgcg gttgccgaat cagtaaaagc ggcgtcagac atttatgcgc
     1621 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
     1681 acagcgaacc gtatgcaggt ggctggatct ttaaaatcaa agccagcgat gaaagcgaac
     1741 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagacgag taacggcttt
     1801 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
     1861 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
     1921 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
     1981 gcgcaacagc aagaaatgct gaatgccgtt ggcgcacaat cgttaaacgc gctgaccggc
     2041 cagattgtac cgaaagatat tcagcttgcg accccgccgc aggttggcgc accggcgacc
     2101 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
     2161 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
     2221 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
     2281 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
     2341 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
     2401 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccgatgtgca tccgcaaacg
     2461 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
     2521 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
     2581 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
     2641 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
     2701 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
     2761 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
     2821 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
     2881 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
     2941 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
     3001 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
     3061 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
     3121 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
     3181 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagcgtgct gctgggcgat
     3241 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
     3301 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctaccac
     3361 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
     3421 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
     3481 ccaatcacct ggccggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
     3541 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
     3601 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
     3661 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
     3721 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
     3781 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
     3841 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
     3901 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
     3961 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
     4021 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
     4081 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
     4141 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
     4201 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
     4261 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
     4321 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
     4381 gaagaaaccg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
     4441 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
     4501 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
     4561 caggtgaaag ccgctgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
     4621 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
     4681 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
     4741 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgact
     4801 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
     4861 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
     4921 gaatatttac cctcaccccg gccctctccc tgaaagggcg aggggaaaag cgtgccaaca
     4981 ttgaagattg agccagtttg ttagcaatct caaagatacg tcaacgaatt aatttttctc
     5041 ggaaaaacaa atggctatag cacttgtgac cgatggcagt cgcggcatcg ggcgggcaac
     5101 tgcattactg ttggcgaaag aagagtatac ggtggcggtt aattatcaac aaaacctcca
     5161 tgcggcgcag gaagtggtga acttaatcac gcaagccggt ggcaaggcat tcgtgctcca
     5221 ggcggatatc agcgacgaaa atcaggtcat cgcaatgttt acagcaatcg atcagcacga
     5281 tgaaccgcta gcagcgctgg tcaataacgc cgggatcttg tttacccagt gcaccgttga
     5341 aaaccttacc gcagagcgaa tcaaccgagt actttccacc aacatgacgg gatattttct
     5401 ctgctgccgc gaggcggtaa aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat
     5461 cgtcaatgtc ttttcggtgg cctcacggtt gggttcgcca ggggaatatg ttgattacgc
     5521 ggcatcgaaa ggggcgattg atacgttaac caccggacta tcgctggaag tcgccgcgca
     5581 gggggtccgc gttaactgcg tgcggccagg gtttattttt accgaaatgc acgccagcgg
     5641 cggcgagcct ggacgcgtcg atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca
     5701 ggcagaagag gtcgcgcagg ccattgtctg gctactaagt gataaagcct cttacgtcac
     5761 gggaagtttt atcgatttgg cgggcgggaa ataaaacagg gaagttgtct gaccggatgc
     5821 aacaagtatt gcatccggta cttcatcgac ttaaagcttc tcgccgttgc tggtaatcac
     5881 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
     5941 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtgg tgaacgatac
     6001 gcagtcgatg cagccccacg gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc
     6061 ttttttcatc tcttcgatat gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct
     6121 gccatcttct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct
     6181 ctgataacgc tcatacagtt cgcaaagggc atagcgcaga cctactggat caatctgcca
     6241 gccccagtca gacgctttaa catacgggtt cggtacgctg ccttcgaaac cagagatcgc
     6301 atcgccggtg ccgccttcgg ccttcactgc gttggtcatg taatagctga aaccaagata
     6361 atcgcaggtg ccttcgcgca gtacatcgag atcgccgtct tccattttga tgttaaatcc
     6421 gcgacgctcc cactcgttca acacatagga cgggtaatag ccgcgtagct gcacatcggt
     6481 aaagacgtag cgttcgcgca tcgactcctg agcgaacatc acatcgtccg ggttacagca
     6541 gtagggatag agcggcacca tcgccagcat acagccgact ttcatctccg ggttaatgcg
     6601 acgcgcagct ttcaccgcca gggcgctggc gacaaactgg tgatgcagca cctgatacat
     6661 cgtctcttcc gggttctcat gctcggtata aaccacgccg gagcagcagt aaccgaacag
     6721 cggtgcacgc cagttacgct ggttgttaat ttcgttgaag gtcatccagt atttgacttt
     6781 atgcttatag cgttcaaaca ccacttccgc gaaacggaca aagaaatcaa ccactttacg
     6841 gttggtccag ctaccgtatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
     6901 gatcaccggt tcgatgttgt atttcagcag ttcatcgaac atatcatcgt agaacttcag
     6961 cccttcttcg tttggctgag cttcatcgcc ttttggaaaa atgcgcgtcc aggctatgga
     7021 agtccgaaaa catttgaagc ccatttcggc aaatagcttg atgtcttcct tgtagtgacc
     7081 ataaaaatca acggcttcat ggtttggata gtatttgcct ggcaagactt ctttggtgat
     7141 ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca caaatgctcg gccctttgcc
     7201 gcctttgttc cagccgcctt cgacctgatg agcggcaact gcgccgcccc ataagaaatc
     7261 tttctgtaag gtgagttttt tcactatcat aggctccaga tgagtcaatt cttacaggaa
     7321 atagtaccat gcagccaaac gacatcactt tttttcaacg tttccaggat gacattctgg
     7381 ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa acgggtgatg
     7441 tgcttcgtgt cgggcgtttt gaagatgacg gttatttttg cacgattgaa gtcaccgcaa
     7501 cctcaaccgt aacgctggat acgctgacgg aaaaacatgc agagcaggaa aatatgaccc
     7561 tgactgaact gataaaggtc attgccgaca tctatcccgg tcagacacag ttttatgtga
     7621 ttgaatttaa atgtctttaa attgaactca cgcctgttag ttgaaatttc gcatttaatt
     7681 tcatgatttt taggagatta gtctcaaatc agaatttatt ttagctaaca ggtgttcact
     7741 ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttc tccggagtaa
     7801 gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga aattgccaac
     7861 agcgtcagtc acggcattgg gttggtgttt ggtatcgttg ggctggtgtt gctactggtt
     7921 caggcggtgg atctcaatgc cagcgccaca gcgataacca gttacagcct ctatggcggc
     7981 agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcctca tcaacgggca
     8041 aaaatgtggc tgaagaaatt tgaccattgc gctatttacc tgttgattgc cggaacctac
     8101 acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat gattgttatc
     8161 tggagcctgg cattgctggg tattctgttt aaactgacca tcgcgcaccg attcaaaatt
     8221 ttatctctgg tgacctatct ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg
     8281 gcagttaagc tcgcggcggg cagcgttacc ttattggcgg taggcggcgt ggtttattcg
     8341 ctcggagtga ttttctatgt ctgcaaacgc attccctaca atcatgccat ctggcacggc
     8401 ttcgtgctcg gcggcagtgt gtgccacttt ctggcgatct atttgtatat tgggcaggcg
     8461 taatgggtat gcctttgtag gcctgaaaag acgcgtcagc gtcgcatcag gcatcggagc
     8521 acaactgccg gattcggcgt gaacgcctta tccgatctac gggcctcgtc cctgttgtga
     8581 taaaggcatc aaatcccaga catgataaga cgcgtcagcg tcgcatcagg cgataaatgc
     8641 ctgatgcgat aaagagattt actcttcgag cgaatacggc agcggctcaa tatgcaatgt
     8701 attcgcatcg tcgcgtacgc ggaagatgct atccggttcc atatcgttat tcatgacgac
     8761 ctgtaccacg acctgaccat cttccagttt taccgcagcc agcaccgtac cggtacgacg
     8821 ccagttctcg cccattttca gctctaagtc ttcaccagct tccggcagtc ggctggcgct
     8881 acctgccagc aaccagagcg cacgtttatt ggcaccacgg aattttgctc gcgccaccat
     8941 ctcttgtccg gtataacagc ctttcttaaa gctgataccg cccagcgcct ggagattggt
     9001 cgcctgtggg ataaactgcc cgctgttggc ggcatcaatc accgggaaac ccgcttcaat
     9061 gtttaatgcc agccactgtt ggctattgtt cagttccgct tcaccgcgca gtttatcggt
     9121 cagcatatta gcagtagctt catcggttac gatcaggaaa cgttctgccg ggtattcaaa
     9181 ccatagcaaa gtggtcgcgc cttctttaac tacctgtttt tctttcgaag gcagttcact
     9241 aaagatattt gccagcgcgg cgcgcgcctg aaaaccggca acaccaagca gcacatgctc
     9301 gtcgtctggc gcgatggtca ctttagagaa caccgcatat tttttcagtt cagtcagctg
     9361 cggttcacgc acgctgcgcc gttcaatcca cgcaaagcca tcgccgtcgc ggaacagacg
     9421 taaattgctc cacattttgc ctttggcgtc gcaatgggcg gcgagcaggt gctgatcttc
     9481 tgccatctgg ctgacatctg ctgtcacctg accctgcata tatttttcgc tgtccgcgcc
     9541 agtaatggtg gcaagcgccc agtcatcaag cgtcatcagc gtcagcggta aacgggcaga
     9601 agccgtaggc tgacggggag gaaaaggtgt aaaagccata atagagtcct gagttgctta
     9661 acggcgtatt aatgactaat ggtaaaagag ctatgaggca atgcaagtgc tttcactacg
     9721 cgatctgtgc cttctgcgag gcgacttcca aaaaaatgaa gccattgaat tgttgtgaat
     9781 taatttcgtg atcgcgatgg tgtttactga tattcccgta aaagaaggcg aaaaacacgt
     9841 tacaatagcg gggtactgga ttttcgtgag acaggaagaa gatggacatt aacaacaaag
     9901 cccgcattca ttgggcatgc cgccgtggta tgcgcgaact cgatatttca atcatgccgt
     9961 ttttcgaaca tgagtacgac agcttaagcg atgacgaaaa acgcatcttt attcgtctgc
    10021 tggaatgtga cgatccggac ctgtttaact ggctgatgaa tcacggtaaa ccggccgatg
    10081 cagaactgga aatgatggtc cgactcatcc agacacggaa ccgggaacgt ggtcctgtgg
    10141 caatctgatt tgcgcgtctc ctggcgcgca cagtggcttt ccttgctgat tcatgggctg
    10201 gttgccgctg ttattttact catgccctgg ccactcagtt acaccccgtt atggatggtg
    10261 ttactttcgc tggtggtgtt tgattgcgtt cgcagccagc ggcgtattaa tgctcgccag
    10321 ggggaaattc gcttgttgat ggacgggcgt ttgcgttggc aagggcagga gtggagcatc
    10381 gtcaaagcac cgtggatgat taagagcggc atgatgctgc gtttacgttc tgatggcggt
    10441 aaacggcaac atttatggct ggcagccgac agcatggacg aagccgaatg gcgggattta
    10501 cggcggattt tgttgcaaca agagacgcaa agataaagat gacggcaaag catatgcgct
    10561 aagccgtcag tgagatcagg cgtaatgctc tgccatttcg ttgaggattt gctcgcacca
    10621 gctctgaata cgctcgtcgc taaggtcata ctggttagtt tcatccagcg ccagacccac
    10681 gaacagttgc ccgtcagcaa tcaccggttt cgggctggta aattcatatc cttccgttgg
    10741 ccagtagccg acgaacttca cgcctttggt cgagagtttg tcatgcagca taccgagcgc
    10801 atcgaggaac cactcgccgt atcccagttg atcgccaagc ccatacagcg caacaatttt
    10861 accttcaagg ttcaggtcgt cgagctgatc ccagacggct tcccagtctt cctggatttc
    10921 accaaaatcc caggtcggga tacccagaat gagcacatcg tactgctcca ttaatttcgg
    10981 ggagtcgtcc ttgaggttat gtaaggtcac cagttctggg ccgataatat cgcggatttt
    11041 ttctgccgcc atttcggtgt aacaggtgct ggaaccgtaa aaaagaccca tattcataac
    11101 gtaagcttct cgattctgct gtggcttttg cagctagtgt atcagaatcg ctttagtcac
    11161 ggcacaataa ggcataatgg atcaaatgag tgaccataag gggcgcaagt gaaacaggat
    11221 ctggcacgca tcgagcagtt tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat
    11281 acgttgaacg cttaccgtcg cgatctgtca atgatggtgg agtggttgca tcaccgcggg
    11341 ttgacgctgg cgacggcgca aagtgacgat ttgcaggcat tactggcaga acggctggag
    11401 ggcgggtata aagccaccag ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag
    11461 tatctttatc gcgaaaagtt tcgtgaagac gatcccagtg cgcatctcgc ttcaccgaaa
    11521 ttgccccagc gtttgccaaa agatttaagc gaagcgcagg tcgaacgttt attacaggcg
    11581 ccattaattg atcagccact ggagctacgc gataaagcca tgcttgaagt gttgtatgct
    11641 accgggctgc gtgtctctga actggtcggg ctgacaatga gtgatatcag cctgcgtcag
    11701 ggcgtagtgc gggtcattgg taaaggcaac aaagagcgcc tggtaccgtt aggtgaagag
    11761 gcggtttact ggctggaaac ctatctggaa catgggcgtc cgtggctgct gaatggtgtg
    11821 tcaattgacg tgttgtttcc cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg
    11881 caccgcatta aacattatgc cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat
    11941 gtgttgcgtc acgcttttgc cactcattta ttaaatcatg gtgcggattt acgcgtggtg
    12001 cagatgctac tgggccacag cgatctttcc accacgcaaa tttatacgca tgtcgctacc
    12061 gagcgtctgc ggcaacttca tcaacagcat cacccgcggg cgtgatgtct gaaaagaacg
    12121 ggaagattta tgaagaaagg ttttatgttg tttactttgt tagcggcgtt ttcaggtttt
    12181 gctcaggctg atgacgcggc aattcaacaa acgttagcca aaatgggcat caaaagcagc
    12241 gatattcagc ccgcgcccgt agctggcatg aagacagttc tgactaacag cggcgtgttg
    12301 tacatcaccg atgatggtaa acatatcatt caggggccaa tgtatgacgt tagcggcacg
    12361 gctccggtca atgtcaccaa taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag
    12421 atgatcgttt ataaagcgct gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc
    12481 tgtggttact gccacaaact gcatgagcaa atggcagact ataacgcgct ggggatcacc
    12541 gtgcgttatc ttgctttccc gcgccagggg ctggacagcg atgcagagaa agaaatgaaa
    12601 gctatctggt gtgcgaaaga taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc
    12661 gtcgcaccag ccagctgcga cgtggatatt gccgaccatt acgcacttgg cgtccagctt
    12721 ggcgttagcg gtactccggc agttgtgctg agcaatggca cacttgttcc gggttaccag
    12781 ccgccgaaag agatgaaaga attcctcgac gaacaccaaa aaatgaccag cggtaaataa
    12841 ttcgcgtgaa acaacagata caacttcgtc gccgtgcagt cgatgaaacg gcagacttgc
    12901 ccgctgaatt gcctcccttg ctgcgccgtt tatacgccag ccggggcgtg cgcagtgcgc
    12961 aagaactgga acgcagtgtt aaagggatgc tgccctggca gcaactgagc ggcgtcgaaa
    13021 aggccgttga gatcctttac aacgcctttc gcgaaggaac gcggattatt gtggtcggtg
    13081 atttcgacgc cgacggtgcc accagcacgg ctctaagcgt gctggcgatg cgctcgcttg
    13141 gttgcagcaa tatcgactac ctggttccaa accgtttcga agacggttac ggcttaagcc
    13201 cggaagtggt cgatcaggcc catgcccgtg gtgcgcagtt aattgtcacg gtggataacg
    13261 gtatttcctc ccatgcgggg gttgaacacg ctcgctcgtt gggcattccg gttattgtta
    13321 ccgatcacca tttgccgggc gaaacattac ccgcggcgga agcgatcatt aaccctaact
    13381 tgcgcgactg taatttcccg tcgaaatcac tggcaggcgt gggtgtggcg ttttatctga
    13441 tgctggcgct gcgcaccttt ttgcgcgatc agggctggtt tgatgagcgc ggcatcgcaa
    13501 ttcctaacct ggcagaactg ctggatctgg tagcgctggg gacagtggcg gacgtcgtac
    13561 cgctggacgc taataatcgc attctgacct ggcaggggat gagtcgcatc cgtgccggaa
    13621 agtgccgtcc ggggattaaa gcgctgcttg aagtggcaaa ccgtgatgca caaaaactcg
    13681 ccgccagcga tttaggtttt gcgctggggc cacgtctcaa tgctgccgga cgactggacg
    13741 atatgtccgt cggtgtggcg ctcttgctgt gcgacaacat cggcgaagcg cgcgtgctgg
    13801 caaatgaact cgatgcgcta aaccagacgc gaaaagagat cgaacaagga atgcaggttg
    13861 aagccctgac cctgtgcgag aaactggagc gcagccgtga cacgctaccc ggcgggctgg
    13921 caatgtatca ccccgaatgg catcaggggg ttgtcggtat tctggcttcc cgtattaaag
    13981 agcgttttca ccgtccggtt atcgcctttg cgcccgcggg tgatggtacg ctgaaaggtt
    14041 ctggtcgctc cattcagggg ctgcatatgc gtgatgcgct ggagcgatta gacacactct
    14101 accctggcat gatgctgaag tttggcggtc atgcgatggc ggcgggtttg tcgctggaag
    14161 cggataaatt cgaactcttt caacaacggt ttggcgaact ggttactgag tggctggacc
    14221 cttcgctatt gcaaggcgaa gtggtgtcag acggtccgtt aagcccggcc gaaatgacca
    14281 tggaagtggc gcagctgctg cgcgatgctg gcccgtgggg gcagatgttc ccggagccgc
    14341 tgtttgacgg tcatttccgg ctgctgcaac agcggctggt gggcgaacgt catttgaaag
    14401 tcatggtcga accggtcggc ggcggtccgc tgctggatgg tattgctttt aatgtcgata
    14461 ccgccctctg gccggataac ggcgtgcgcg aagtgcaact ggcttacaag ctcgatatca
    14521 acgagtttcg cggcaaccgc agcctgcaaa ttatcatcga caatatctgg ccaatttagc
    14581 gtcatcttct ctataaaaaa gagcgtggat tgggtacaat cccgctctta tcaccgcatt
    14641 ttgactagct caataaaaga aatcagacca tgtttgaaat taatccggta aataatcgca
    14701 ttcaggacct cacggaacgc tccgacgttc ttagggggta tctttgacta cgacgccaag
    14761 aaagagcgtc tggaagaagt aaacgccgag ctggaacagc cggatgtctg gaatgaaccc
    14821 gaacgcgcac aggcgctggg taaagagcgt tcctccctcg aagccgttgt cgacaccctc
    14881 gaccaaatga aacaggggct ggaagatgtt tctggtctgc tggaactggc tgtagaagct
    14941 gacgacgaag aaacctttaa cgaagccgtt gctgaactcg acgccctgga agaaaaactg
    15001 gcgcagcttg agttccgccg tatgttctct ggcgaatatg acagcgccga ctgctacctc
    15061 gatattcagg cggggtctgg cggtacggaa gcacaggact gggcgagcat gcttgagcgt
    15121 atgtatctgc gctgggcaga atcgcgtggt ttcaaaactg aaatcatcga agagtcggaa
    15181 ggtgaagtgg cgggtattaa atccgtgacg atcaaaatct ccggcgatta cgcttacggc
    15241 tggctgcgta cagaaactgg cgttcaccgc ctggtgcgta agagcccgtt tgactccggc
    15301 ggtcgtcgcc acacgtcgtt cagctctgcg tttgtttatc cggaagttga tgatgatatt
    15361 gatatcgaaa tcaacccggc ggatctgcgc attgacgttt atcgcgcgtc cggcgcgggc
    15421 ggtcagcacg ttaaccgtac cgaatctgcg gtgcgtatta cccacatccc gaccgggatc
    15481 gtgacccagt gccagaacga ccgttcccag cacaagaaca aagatcaggc catgaagcag
    15541 atgaaagcga agctttatga actggagatg cagaagaaaa atgccgagaa acaggcgatg
    15601 gaagataaca aatctgacat cggctggggc agccagattc gttcttatgt ccttgatgac
    15661 tcccgcatta aagatctgcg taccggggta gaaactcgca acacgcaggc cgtgctggac
    15721 ggcagcctgg atcaatttat cgaagcaagt ttgaaagcag ggttatgagg aaccaacatg
    15781 tctgaacaac acgcacaggg cgctgacgcg gtagtcgatc ttaacaatga actgaaaacg
    15841 cgtcgtgaga agctggcgaa cctgcgcgag caggggattg ccttcccgaa cgatttccgt
    15901 cgcgatcata cctctgacca attgcacgca gaattcgacg gtaaagagaa cgaagaactg
    15961 gaagcgctga acatcgaagt cgccgttgct ggccgcatga tgacccgccg tattatgggt
    16021 aaagcgtctt tcgttaccct gcaggatgtg ggtggccgca ttcagctgta cgttgcccgt
    16081 gacgatctgc cggaaggcgt ttacaacgag cagttcaaaa aatgggacct cggcgacatc
    16141 ctcggcgcga aaggtaaact gttcaaaacc aaaaccggcg aactgtctat ccactgtacc
    16201 gagctgcgtc tgctgaccaa agcactgcgt ccgctgccgg ataaattcca cggcttgcag
    16261 gatcaggaag cgcgctatcg tcagcgttat ctggatctca tctctaacga tgaatcccgt
    16321 aacaccttta aagtacgctc gcagatcctc tctggtattc gccagttcat ggtgaaccgc
    16381 ggctttatgg aagttgaaac gccgatgatg caggtgatcc caggcggtgc cgctgcgcgt
    16441 ccgtttatca cccaccataa cgcgctggat ctcgacatgt acctgcgtat cgcgccggaa
    16501 ctgtacctca agcgtctggt ggtcggtggc ttcgagcgtg tattcgaaat caaccgtaac
    16561 ttccgtaacg aaggtatttc cgtacgtcat aacccagagt tcaccatgat ggaactctac
    16621 atggcttacg cagattacaa agatctgatc gagctgaccg aatcgctgtt ccgtactctg
    16681 gcacaggata ttctcggtaa gacggaagtg acctacggcg acgtgacgct ggacttcggt
    16741 aaaccgttcg aaaaactgac catgcgtgaa gcgatcaaga aatatcgccc ggaaaccgac
    16801 atggcggatc tggataactt cgactctgcg aaagcgattg ctgaatctat cggtatccac
    16861 gttgagaaga gctggggtct gggccgtatc gttaccgaga tcttcgaaga agtggcagaa
    16921 gcacatctga ttcagccgac cttcattact gaatatccgg cagaagtttc tccgctggcg
    16981 cgtcgtaacg acattaaccc ggaaatcaca gaccgctttg agttcttcat cggtggtcgt
    17041 gaaatcggta acggctttag cgagctgaat gacgcggaag atcaggcgca acgcttcctg
    17101 gatcaggttg ccgcgaaaga cgcaggtgac gacgaagcga tgttctacga tgaagattac
    17161 gttaccgcac tggaacatgg cttaccgccg acagcaggtc tggggattgg catcgaccgt
    17221 atggtaatgc tgttcaccaa cagccatacc atccgcgacg ttattctgtt cccggcgatg
    17281 cgtccggtaa aataagcatt acgttatgct cacaaccccg gcaaatgtcg gggtttttta
    17341 attgtgctgc gcgaaagcag acaacaattt tcttgcttca ctattggctg cctgcatcac
    17401 catccacgga ctgaacgccc acggtgtggc atcaataccg tgtaatacat ctgctaaatc
    17461 acaccattga taatccatca cttcatcatc gttgatctgt aacgcactgg tggtgcgtgc
    17521 ggcaaatacc ggacacactt cattttccac aatgccattc ggatcggtgg cgcgatagcg
    17581 aaagtcagga tagatagatt caggaggcgt aatttccacg ccaagctcat aacggcaacg
    17641 gcggatcacc gcgtcttcgt tgctttctcc cagttgtggg tgcccacaaa ccgagttagt
    17701 ccacacgcca ggccacgctt ttttgctcag tgcgcggcgg gtaactaata attgtccttt
    17761 ggcattaaac agccaactgg agaacgcgag atgtaagcgg gtgtctgccg tgtgtgcggc
    17821 atacttttcc agcgtacccg tgggaactcc ctgtgcattc aataaaatga cgtgttccgt
    17881 ttgcataatt tctcacatgt aattctgatc actcatcatt atagataatt gaagagtgaa
    17941 atgattaacc aagtgagcgt ttatcgacaa ccgcccgttt tgagcggatg ccgacaggta
    18001 aaaaccattt aaaaaagtag catcatatat ttgaagcgac tttcaatggc cattgcaaca
    18061 cagtcagaaa tgacaggaat agagagagta aaaagcaaca gcagactgaa tataacgcgc
    18121 attaaaaaat tcatattatt ctccatgctc atttttcttc agcttgcgca tcagaagcca
    18181 catacgtact gtcctgactt ttaaatcttt atcagagacg gattccttca ccaggtcagc
    18241 atgatgctgg tagccattaa agaagacatt cagcacaacg gcgctaaggg ttgcgagcat
    18301 aatgccgcta tgcagcaacg gttgcagtac ggccggtaac ttagaaaaga aatcgtgaga
    18361 gagcgtcgga gtcatgccaa cgccgagact gatcgccaca atatagaggt tgtaacggtt
    18421 ggtggtgtag ttacagcgcg acagaattcg aatccctgtc gccagtacca tgccgaacat
    18481 caccagccca gcaccgccca gcacaaattg cggaatggag gcgaccagca ccgccatttt
    18541 tggcaccatg ccaaacagga ttaatataat tcccgaagaa atacacaccc agcggctatg
    18601 aacgcgcgtc acgctaacca ggccaacgtt ttgtgaaaat gacgtgtgga ggaagctgtt
    18661 aaacgttccg cctatcattg tccctacgcc atcgacacgc agcccgcgaa taatatcgtg
    18721 tgaagagagt ttacgaccga ctatttcacc cagtgccagg aacatcccca tcgactcgat
    18781 aaacacgatg attaacacgg cagtcatggt cagaatggaa acgggatcga aaatcggcat
    18841 accaaatgac atcggcgtga caatcgcaaa ccatgaagca tcatgtagcc cggataaatt
    18901 gacttcattc atcatccacg aaagtaaaaa gccaaataca atccccagta atacggcgac
    18961 gttggacata aatcctttcg catagcgagt aatgagcaag ataaaaatta agacagcaaa
    19021 ggagatacct aaataaacgg gattaccata ttgcggattc cctttacctc cggcggccca
    19081 gtcaataccc acctgaatga tgctaagccc aatagaagta ataaccacac cggtaaccag
    19141 tggcgggaat aaaggcatca agcgaccaat aaatggcgct aataatgtgg tgataaaacc
    19201 cgcggcgata gtggcaccaa atatccccag caggccgata tccgggttca tcccaatggc
    19261 tatcattggt gttacagcag caaaggtcac cgacataatc accggcaggc ggatccccat
    19321 aaagcggccg ataccgatac attgcaataa tgtgacgatc ccgcagcaaa agagatccga
    19381 gctaatgagc atcgcaatag catcttttga gaggcccagt cggtcaccaa tcataagagg
    19441 aacagcgact gcacctgcgt acatgacaag gacgtgttgc agaccgagta tgatcagctt
    19501 tcctggtgat aatatgcggt caacctcatc agttgggcgg tcttgcccag aagatgaggg
    19561 aagttgggaa tctatggcgc tcatcagtgt ttctcctttc gcccgccaga gtaaaaagca
    19621 accgtttacg ttaatgaaac tgtctaaaca catgctcgtg gagtgccagt ttttgcgagg
    19681 aaggggagtt agagacagga attgcggagg caatcacata atcgtgctta aaccacgaaa
    19741 atgacaagag aacctgacaa tggaattatc tgtaattttc tcatattgat atttcattaa
    19801 tcattctggc atgtcgcact ctcgcattta atcgttttta tctggatagc gctcttttga
    19861 tcggcaatgt tgaaataatg aaagcggcat agattgaggg gcaataattt gaactcagtc
    19921 gcaaaattat aattaaaacg gtcatgctac ctaatatatg cccgtttatt gcatcgcgaa
    19981 atttgagaag acataactca
//
LOCUS       DAZSBF010000063        19970 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZSBF010000063 DAZSBF010000000
VERSION     DAZSBF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.064"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(226..1038)
                     /locus_tag="ACHXWD_001772"
     CDS             complement(226..1038)
                     /locus_tag="ACHXWD_001772"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR7620706.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1300..2061
                     /gene="udp"
                     /locus_tag="ACHXWD_001773"
     CDS             1300..2061
                     /gene="udp"
                     /locus_tag="ACHXWD_001773"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR7620707.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2202..3629
                     /gene="rmuC"
                     /locus_tag="ACHXWD_001774"
     CDS             2202..3629
                     /gene="rmuC"
                     /locus_tag="ACHXWD_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR7620708.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3724..4479
                     /gene="ubiE"
                     /locus_tag="ACHXWD_001775"
     CDS             3724..4479
                     /gene="ubiE"
                     /locus_tag="ACHXWD_001775"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR7620709.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4493..5098
                     /gene="ubiJ"
                     /locus_tag="ACHXWD_001776"
     CDS             4493..5098
                     /gene="ubiJ"
                     /locus_tag="ACHXWD_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR7620710.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5095..6735
                     /gene="ubiB"
                     /locus_tag="ACHXWD_001777"
     CDS             5095..6735
                     /gene="ubiB"
                     /locus_tag="ACHXWD_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR7620711.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6814..7083
                     /gene="tatA"
                     /locus_tag="ACHXWD_001778"
     CDS             6814..7083
                     /gene="tatA"
                     /locus_tag="ACHXWD_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR7620712.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7087..7602
                     /gene="tatB"
                     /locus_tag="ACHXWD_001779"
     CDS             7087..7602
                     /gene="tatB"
                     /locus_tag="ACHXWD_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR7620713.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7605..8381
                     /gene="tatC"
                     /locus_tag="ACHXWD_001780"
     CDS             7605..8381
                     /gene="tatC"
                     /locus_tag="ACHXWD_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR7620714.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8423..9205
                     /gene="tatD"
                     /locus_tag="ACHXWD_001781"
     CDS             8423..9205
                     /gene="tatD"
                     /locus_tag="ACHXWD_001781"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR7620715.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9202..9690)
                     /gene="rfaH"
                     /locus_tag="ACHXWD_001782"
     CDS             complement(9202..9690)
                     /gene="rfaH"
                     /locus_tag="ACHXWD_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR7620716.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9857..11350
                     /gene="ubiD"
                     /locus_tag="ACHXWD_001783"
     CDS             9857..11350
                     /gene="ubiD"
                     /locus_tag="ACHXWD_001783"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR7620717.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11397..12098
                     /gene="fre"
                     /locus_tag="ACHXWD_001784"
     CDS             11397..12098
                     /gene="fre"
                     /locus_tag="ACHXWD_001784"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR7620718.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12288..13451)
                     /gene="fadA"
                     /locus_tag="ACHXWD_001785"
     CDS             complement(12288..13451)
                     /gene="fadA"
                     /locus_tag="ACHXWD_001785"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR7620719.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13461..15650)
                     /gene="fadB"
                     /locus_tag="ACHXWD_001786"
     CDS             complement(13461..15650)
                     /gene="fadB"
                     /locus_tag="ACHXWD_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR7620720.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15840..17171
                     /gene="pepQ"
                     /locus_tag="ACHXWD_001787"
     CDS             15840..17171
                     /gene="pepQ"
                     /locus_tag="ACHXWD_001787"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR7620721.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17171..17785
                     /locus_tag="ACHXWD_001788"
     CDS             17171..17785
                     /locus_tag="ACHXWD_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR7620722.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17824..19275
                     /gene="trkH"
                     /locus_tag="ACHXWD_001789"
     CDS             17824..19275
                     /gene="trkH"
                     /locus_tag="ACHXWD_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR7620723.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19287..19832
                     /gene="hemG"
                     /locus_tag="ACHXWD_001790"
     CDS             19287..19832
                     /gene="hemG"
                     /locus_tag="ACHXWD_001790"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR7620724.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4644 a         5067 c         5443 g         4816 t
ORIGIN      
        1 tgttgataat aattaatgtt tgtttgtgaa tgggagttga tggtaaatct ctaaggtgtc
       61 ttccattgaa gagactcatt tatgactatg acttgccgac tttcttaagg tcaaatagcg
      121 gagtcgtaat ctccaatcaa ttcatactga ttattttttg ggggacagag atggcagcaa
      181 aacgcactcg tttactcacc atctcaaata tagactaaga ggaaactaac tccgcttctc
      241 tccatactgc ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg
      301 atagctcggg cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc
      361 tgctttcgca ttagcagccc gcagcgcctg gcgcatggtt tcaacgctct cctgcggaat
      421 gctgttatcc tgaccgccat ataagccgag aatcggcgcg ttaagatcgg ttgcgatatc
      481 aacaggttgt ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac
      541 tgcagctttt agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca
      601 gaatccggtg atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcggc
      661 atgatcgaga tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa
      721 cgtggggata tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat
      781 agccagatac ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc
      841 aaaaatttcc tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc
      901 atggtaagca ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc
      961 gtcgtccagg gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga
     1021 ttgttgtgtt gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac
     1081 tatagtcagc atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc
     1141 agaatgaaga gtgatttatg tgatttgaat cacttttggt gggtaaattt atgcaacgca
     1201 tttgcgtcat ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt
     1261 tgcttcttct gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt
     1321 catctcggcc tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac
     1381 ccggatcgtg tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac
     1441 cgcgaattca ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc
     1501 ggtatcggcg gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc
     1561 accttcctgc gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc
     1621 ctggttacca cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa
     1681 ttcccggctg tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatccatt
     1741 ggcgcgacaa ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa
     1801 cgttacgata cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg
     1861 caggcgatgg gcataatgaa ctatgaaatg gaatctgcaa ccctgctgac catgtgtgca
     1921 agtcagggcc tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag
     1981 atcccgaatg ctgagacgat gaaacaaact gaaagccaag cggtgaaaat cgtggtggaa
     2041 gcggcgcgtc gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt
     2101 tgtatttttg cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca
     2161 attctatttt gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg
     2221 tttacgcagt tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac
     2281 atgcgcagca aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg
     2341 cggcaaaaca acaaattacc caaagcgagc actggcgtgc agagtgcgag ttactcaata
     2401 acgaagtgcg cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa
     2461 ccacgcggat ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca
     2521 gcgagcagcg cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca
     2581 atcgccgggt tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg
     2641 aacaactgga cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac
     2701 gccataccct gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg
     2761 aagcgatcaa cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg
     2821 aggtagtatt gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa
     2881 cccaggtcag catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc
     2941 tgccgcaggg aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct
     3001 attttaacgc cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg
     3061 tgcgtaacca tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa
     3121 ctctggatta cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc
     3181 gccagccgga gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta
     3241 cgctgctggt ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgca
     3301 acgcccagca aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg
     3361 atgacatgtc cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa
     3421 tgaaaaaact ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt
     3481 taggagtaga aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccaga
     3541 atgaagagta tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg
     3601 atgatgaata taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat
     3661 ctagggcatc gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt
     3721 gagatggtgg ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa
     3781 caaaaagcgg atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg
     3841 aatgatttga tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc
     3901 ggcgtacgcc gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg
     3961 aaattctccc gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc
     4021 atgctcaaaa tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat
     4081 gttcaggcga acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt
     4141 tcgtttggtc tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg
     4201 ctgaaacccg gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg
     4261 agcaaagcct atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg
     4321 aacgacgccg acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat
     4381 accctgaaag ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg
     4441 gcaggggttg tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt
     4501 taaaccttta gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc
     4561 cgcgctgaaa acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg
     4621 cttttcgacg tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg
     4681 ggcaggcgat gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga
     4741 tcgccagcag cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca
     4801 ggtggtgcaa aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct
     4861 ggccccttat accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc
     4921 aaagttcctg catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga
     4981 agagtggcgt atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc
     5041 cgtcgagcgt gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg
     5101 ccaggtgaag tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat
     5161 gaactgatcc ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc
     5221 tggatgccaa atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa
     5281 gaactggggc cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt
     5341 ccaccgcata ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc
     5401 aagctggcga agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt
     5461 gacgattttg aaatcaagcc actggcttct gcttctatcg cccaggttca taccgcgcga
     5521 ttgaaatcga atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtt
     5581 attaaagcgg atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg
     5641 gatggtcgcc gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat
     5701 gaactgaatt tgctgcggga atctgccaac gccattcagc ttcggcgaaa ttttgaagac
     5761 agcccgatgc tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg
     5821 atggagcgca tttacggcat tccggtgtct gatgttgcga cgctggagaa aaatggcacc
     5881 aacatgaaat tgctggcgga acgtggcgtg caggtgttct tcactcaggt ctttcgcgac
     5941 agctttttcc atgccgatat gcaccctggc aacattttcg taagctatga acacccggaa
     6001 aacccgaaat atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa
     6061 cgctatctgg cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag
     6121 ctacacgtcg attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc
     6181 attcgtacgg tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat
     6241 gtactgttaa atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg
     6301 gtgttactcc agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag
     6361 ctcgatttat ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt
     6421 attcctgccc tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca
     6481 gaactgcctg aattggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt
     6541 gataagattg cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt
     6601 ctcggaattg gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgaa
     6661 tgggggctga tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg
     6721 cgcaaaacac gctgattttt tcatcgctca aggcgggtcg tgtaacgtat aatgcggcct
     6781 tgtttaatca tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta
     6841 ttgattattg ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt
     6901 tccgatcttg gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag
     6961 caggataaaa ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg
     7021 gatacgaatc aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg
     7081 taatccgtgt ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc
     7141 gttctggggc cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg
     7201 ttgcgttcac tggcgacaac ggtgcagaac gaactgaccc aggagttaaa actccaggag
     7261 tttcaggaca gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac gcccgaactg
     7321 aaagcgtcga tggatgaact acgccaggcc gcggagtcga tgaagcgttc ctacgttgca
     7381 aacgatcctg aaaaggcgag cgatgaagcg cacaccatcc ataacccggt ggtgaaagat
     7441 aatgaagctg cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc cagttcgccg
     7501 gaacagaagc cagaaaccac gccagagccg gtggtaaaac ctgctgcgga cgctgaaccg
     7561 aaaaccgctg caccttcccc ttcgtcgagt gataaaccgt aaacatgtct gtagaagata
     7621 ctcaaccgct tatcacgcat ctaattgagc tgcgtaagcg tctgctgaac tgcattatcg
     7681 cggtgatcgt gatattcctg tgtctggtct atttcgccaa tgacatctat cacctggtat
     7741 ccgcgccatt gatcaagcag ttgccgcaag gttcaacgat gatcgccacc gacgtggcct
     7801 cgccgttctt tacgccgatc aagctgacct ttatggtgtc gctgattctg tcagcgccgg
     7861 tgattctcta tcaggtgtgg gcatttatcg ccccagcgct gtataagcat gaacgtcgcc
     7921 tggtggtgcc gctgctggtt tccagctctc tgctgtttta tatcggcatg gcattcgcct
     7981 actttgtggt ctttccgctg gcatttggct tccttgccaa taccgcgccg gaaggggtgc
     8041 aggtatccac cgacatcgcc agctatttaa gcttcgttat ggcgctgttt atggcgtttg
     8101 gtgtctcctt tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg attacctcgc
     8161 cagaagactt acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt
     8221 tgctgacgcc gccggatgtc ttctcgcaaa cgctattggc gatcccgatg tactgtctgt
     8281 ttgaaatcgg tgtcttcttc tcacgctttt acgttggtaa agggcgaaat cgggaagagg
     8341 aaaacgacgc tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg
     8401 cggttgtcat atggagtaca ggatgtttga tatcggcgtt aatttgacca gttcgcaatt
     8461 tgcgaaagac cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta atgggctact
     8521 catcaccggc actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc
     8581 gtcctgttgg tcaacggcgg gcgtacatcc tcacgacagc agccagtggc aagctgcgac
     8641 tgaagaagcg attattgagc tggccgcgca gccagaagtg gtggcgattg gtgaatgtgg
     8701 tctcgacttt aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt ttgttgccca
     8761 gctacgcatt gccgcagatt taaacatgcc ggtatttatg cactgtcgcg atgcccacga
     8821 gcggtttatg acattgctgg agccgtggct ggataaactg cctggtgcgg ttcttcattg
     8881 ctttaccggc acacgcgaag agatgcaggc gtgcgtggcg catggaattt atatcggcat
     8941 taccggttgg gtttacgatg aacgacgcgg actggagctg cgggaacttt tgccgttgat
     9001 tccggcggaa aaattactga tcgaaactga tgcgccgtat ctgctccctc gcgatctcac
     9061 gccaaagcca tcatcccggc gcaacgagcc agcccatctg ccccatattt tgcaacgtat
     9121 tgcgcactgg cgtggagaag atgccgcatg gctggctgcc accacggatg ctaatgtcaa
     9181 aacacggttt gggattgcgt tttagagttt gcggaactcg gtgttcttca cactgtgctt
     9241 aatctcttta ttaataagat taagcaatag catggagcga gcctcaccat cgggttcggt
     9301 gaaaatggcc tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat ctcccggata
     9361 aggggttgcc ggatcgacaa tgtctttcgg tttatatacc gatagctgat gaatcaccgc
     9421 cgatgggact atcgctagcg acgcgccaaa gcgcacgaag tggctgacac cgcgggtcgc
     9481 gttgatagtc gtggtatgaa tcacttctgg atcaaattcg acaaacaggt agttgggaaa
     9541 caatggctca ctgactgcag tacgttttcc acgcacgatt ttttccaggg tgatcatcgg
     9601 tgccaggcaa ttcacagcct gtctctcaag gtgttcctgg gcacgttgaa gttgcccgcg
     9661 cttgcagtac agtaaatacc aagattgcat aatgactctt atccgtttaa tcggggcgca
     9721 aggatagcaa aagctttacg ctaagttaat tatattcccc ggtttgcgtt ataccgtcag
     9781 agttcacgct aatttaacaa atttacagca tcgcaaagat gaacgccgta taatgggcgt
     9841 agattaagag gctacaatgg acgccatgaa atataacgat ttacgcgact tcttgacgct
     9901 gcttgaacag cagggtgagc taaaacgtat cacgctcccg gtggatccgc atctggaaat
     9961 cactgaaatt gctgaccgca ctttgcgtgc cggtgggcct gcgctgttgt tcgaaaaccc
    10021 taaaggctac tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat
    10081 gggcatgggg caggaagatg tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct
    10141 gaaagagccc gagccgccaa aaggtttccg cgacctgttt gataaactgc cgcagtttaa
    10201 gcaagtattg aacatgccga caaagcggct gcgtggtgcg ccctgccaac aaaaaatcgt
    10261 ctctggcgat gacgtcgatc tcaatcgcat tcccattatg acctgctggc cggaagatgc
    10321 cgcgccgctg attacctggg ggctgaccgt aacgcgcggc ccgcataaag agcggcagaa
    10381 tctgggcatt tatcgccagc agctgattgg taaaaacaaa ctgattatgc gctggctgtc
    10441 gcatcgcggc ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt
    10501 cccggtttct gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag tcacccccgt
    10561 tccggataca ctttcagagt atgcgtttgc cggattgctg cgcggtacca agaccgaagt
    10621 ggtgaagtgt atctccaatg accttgaagt gcccgccagt gcggagattg tgctggaagg
    10681 gtatatcgaa caaggcgaaa ctgcgccgga agggccgtat ggcgaccaca ccggttacta
    10741 taacgaagtc gatagtttcc cggtatttac cgtgacgcat attacccagc gtgaagatgc
    10801 gatttaccac tccacctata ctggtcgtcc gccggatgaa cccgcggtgc tgggtgtcgc
    10861 actgaacgaa gtgtttgtgc cgattctgca aaaacagttc ccggaaattg tcgattttta
    10921 cctgccaccg gaaggctgtt cttatcgtct ggcggtagtg acaatcaaaa aacagtacgc
    10981 cggacacgcg aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac
    11041 taaatttgtg atcgtttgcg atgatgacgt caacgcacgc gactggaacg atgtgatttg
    11101 ggcgattacc acccgtatgg acccggcgcg ggatactgtt ctggtagaaa atacgcctat
    11161 tgattatctg gattttgcct cgcctgtctc cgggctgggt tcaaaaatgg ggctggatgc
    11221 cacgaataaa tggccggggg aaacccagcg tgaatgggga cgtcccatca aaaaagatcc
    11281 tgatgtcgtc gcgcatattg acgccatctg ggatgaactg gctattttta acaacggtaa
    11341 aagcgcctga tgcgcgttag ttttgcccta tttatttgat ccgacagaga aagcgcatga
    11401 caaccttaag ctgtaaagtg acctcggtag aagctatcac ggatactgta taccgtgtcc
    11461 gaatcgtacc agacgcggcc ttttcttttc gtgctggtca gtatttgatg gtagtgatgg
    11521 atgagcgcga caaacgtccg ttttcaatgg cttcgacgcc ggatgaaaaa gggtttatcg
    11581 agctgcatat tggcgcttct gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc
    11641 tcaaagatca tcaaatcgtg gtcgatattc cccacggaga agcgtggctg cgcgatgatg
    11701 aagagcgtcc gatgattttg attgcgggcg gcaccgggtt ctcctatgcg cgctcgattt
    11761 tattgacggc gttggcgcgt aatcccaatc gcgatatcac catttactgg ggtgggcgtg
    11821 aagagcaaca tctgtatgat ctctgcgagc ttgaggcgct ttcgctgaag catcctggtc
    11881 tgcaagtggt gccggtggtt gaacaaccgg aagcgggctg gcgtgggcgt actggcaccg
    11941 tgttaacggc ggtcttgcag gatcatggta cgctggcaga gcatgatatc tatattgccg
    12001 gacgttttga gatggcgaaa attgctcgtg acctgttttg cagtgagcgt aatgcgcggg
    12061 aagatcgtct gtttggcgat gcgtttgcat ttatctgaga tataaaaacc cgcccctgac
    12121 aggcgggaag aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg
    12181 gcaatcggtg cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc
    12241 aggatcggta gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca
    12301 aacaccgtcg caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg
    12361 tctttgtgtt ccatcagatt cagcaacgtg gtgctgatac gcgcaccgga acaacccagc
    12421 ggatgaccca gcgcgatcgc accaccattg aggttgatct tctcgtcaat ctgctccatt
    12481 agcccgagat ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac
    12541 acgccgatat cgctggcaga aagccccgct tttttcagcg ccagcttcga ggcaggaacc
    12601 gggccgtaac ccataatcga cggatcacaa ccaacgaccg ccatcgaacg cacacgggcg
    12661 cgcggcttaa gacctaattc acgggcgcgg ctttcactca tcaccagcat ggccgcggca
    12721 ccatcagaaa gtgcagaaga tgtacccgcc gttaccgtac cgcttactgg atcaaacgcc
    12781 ggacgcagcg tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta
    12841 aactgcttca ggacgccgtc ggcatcgtga ccaccggtcg ggatgatttc atttttaaat
    12901 gcgcccgact gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc
    12961 atttcacggc tgataccgtg catacgcgcc agcatttctg ccgttaagcc catcatgccc
    13021 gccgctttgg cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc
    13081 acatggccca tatgctccac gccgccaacc agacctgcct gcgcatcgcc agtcatgatc
    13141 attcgtgctg cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc
    13201 gccgggacag agtgcggcac ttctgccagc agcgccgcgt tacgggcgat attaaaaccc
    13261 tgctccagcg tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc
    13321 gccgggttac gcgccagcag gctacgcatt aaatgagcgg agagatcttc tgcacgcacg
    13381 ttacgaaaag caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca
    13441 acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca
    13501 accggaggat agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttca
    13561 tacagcgggc cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg
    13621 gtgtccagcc agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc
    13681 gccatatccg cttccgccgg agtggcgata atgccctctt ccagacagcg caccacttcg
    13741 ttgaccatcg ggatcatcat gcgggcgata atctcttctt cgctgaaatc gcgcttcggc
    13801 tggctcactt ctgccagcag gtcttcaacg gcggcgtctt cttctttctt cggcttacct
    13861 ttgctgtctt ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca
    13921 tcaaacagcg cgtcgatggc atcgcggtaa tctttttgca tccgctgcgg gaagcctgct
    13981 gccatgacag cctgagcgtg atgcgcggta tcaatgccca caacgtccag cagatatgcc
    14041 gggcccatcg gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg
    14101 ccgtcgcgca gcagctggct gaaaccggcg aaatacggga acagcacgcg gttaacaaag
    14161 aagccggggc agtcgttaac cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca
    14221 actttcgcga tggtttcgtc ggagcttttc tcgccgcgaa taatttctac caacggcatt
    14281 cggtggaccg ggttaaagaa gtgcatcccg cagaagtttt ccgggcgttc cagcgcgttg
    14341 gccagttcgc tgataggaat ggttgaagtg ttagacgcca gcacggtatc ctggcgtact
    14401 ttttgttcgg tttctgccag tacggctttt ttcactttcg ggttttcaac aaccgcttct
    14461 accacaatat ccacgcggtc aaatccggcg tagtcgagcg ttgggtggat cgtggagatc
    14521 acgccagcca gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc
    14581 gcggcttcgg tcatgccgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc
    14641 acgcctttcc acgcggactg gtaagcgata ccgccaccca tgatgcctgc gcccagcaca
    14701 gcagcctgtt tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat
    14761 tgatcgttaa ggaaaatgcc gaccagtgcg cgagcttcgt tggtatgcgc cagcgggaca
    14821 aaacttttgt tttccagatt taaggcttct tcacgaccaa aacgggccgc agcttcaatg
    14881 gtttttactg cggtgatggg ggccggataa tgtttccccg ccgtttgcgc gaccattcct
    14941 ttagcgatgg tgaagctcat ggtggcttca atcttgctca gttttagcgg ttccagcttc
    15001 ggctggcgtt tcgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aaccgccttt
    15061 gcgccttcaa ccagtttttc tgctttgact accccatcca ccagaccgat tttcagcgcc
    15121 tgatccgcgc cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc
    15181 atacgtggca tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc
    15241 agaccgatgc gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag
    15301 ccaccaccca gcgcatagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg
    15361 cgattaaaca cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg
    15421 aacagggaca aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc
    15481 agcagcagcc cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg
    15541 gcgacggtcg cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca
    15601 atgccatctt ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc
    15661 tgaatccgca aggtgatctg gtcgtaccag atgcatcgaa gtgtgtattt tgtgttaaaa
    15721 atatgcaaat aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc
    15781 tggatgaacg atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa
    15841 tggaatcact ggcctcgctc tataaaaatc atatagctac cttacaagaa cggactcgcg
    15901 atgcgctggc gcgcttcaag ctggatgcat tacttattca ctccggcgag ctgttcaacg
    15961 tttttctcga cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc
    16021 cggtaactca ggtgccaaac tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt
    16081 ggttctatct gccggttgat tactggcaca acgtcgaacc gctgccgacc tccttctgga
    16141 ctgaagatgt ggaagtgatc gcactgccga aagccgatgg cattggtagc ctgctgcccg
    16201 ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga acgtgcgctg caactgggta
    16261 ttgaggccag caacatcaac ccgaaagggg tgatcgacta cctgcattac taccgctcct
    16321 tcaaaaccga gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc
    16381 atcgtgcggc agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacatcgcct
    16441 atctgaccgc gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta
    16501 acgaacacgc ttcggtgctg cattacacca aactggatca tcaggcaccg gaagagatgc
    16561 gcagcttcct gctggatgcc ggggccgaat ataacggcta tgcggctgac ctgactcgta
    16621 cctggtcggc aaaaagcgac aacgactacg cacagctggt gaaagacgta aatgatgaac
    16681 aactggcgct gatcgccacc atgaaagcag gcgttagcta tgtggattac cacatccagt
    16741 tccatcagcg catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag
    16801 aagcgatggt cgaaaacgat ctcaccggac cgtttatgcc gcatggtatc ggtcatccgc
    16861 tgggcctgca ggtgcatgac gtagccggtt ttatgcagga tgatagcggt acacacctcg
    16921 cggcaccggc aaaatatccg tacctgcgct gcacccgtat tctccagccg ggtatggtgt
    16981 taaccatcga accgggtatc tacttcattg aatcgctact ggcaccgtgg cgtgaagggc
    17041 agttcagcaa gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc
    17101 gtatcgaaga caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga
    17161 aactggcgtg atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat
    17221 caaaaaaagc cgcttcataa cgatgttggc gcataccgat ggcgttgagg cggcgaaagc
    17281 gtttgttgaa tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt
    17341 ggcgggtgcg ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcagg
    17401 aacggcaggt aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattacctc
    17461 tgtggtagtg cgctactacg gcggcatatt gcttggcacc ggtgggttag tgaaagcgta
    17521 tggcggcggc gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac
    17581 cgaatatact ttgcaatgtg aatatcatca gttaaccggc attgaagcgt tgctggggca
    17641 gtgtgacggc aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct
    17701 tccggcggcg aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt
    17761 gcaattgtta gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca
    17821 gagatgcatt ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg
    17881 accatgatta tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt
    17941 acccagacct tttttgtcgc cctcgccatt ggttctatgc tgtggtggcc gaaccgtaaa
    18001 gagaagggcg aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ctggaccgtg
    18061 ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc
    18121 gatgcgtttt ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg
    18181 ctggattcgc tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg
    18241 atggggatca tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag
    18301 ctctatcgcg cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg
    18361 gaaacggcga aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg
    18421 ttgtggtttg ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct
    18481 attggcggct tctcgacaca tgatgccagt atcggttatt tcgacagccc gactattaac
    18541 actatcattg ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg
    18601 ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc
    18661 gtacagttta cgctggtggt tatttgtaca ctcgtactgt ggtttcataa tgtctacagt
    18721 tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc
    18781 gggtttacga ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt
    18841 tcagctttta tcggcggttg cgccgggtca acgggcggtg gcctgaaagt gatccgcatc
    18901 ctgctgctgt ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc
    18961 tatagcatta agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga
    19021 tttttctccg cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc
    19081 gtggatgact tttctgcctt tgcttcagtt gttgcgacat tgaataacct ggggccaggg
    19141 cttggcgtgg ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt
    19201 gccaacatgc tgtttggtcg tctcgaggtc tttacattgc tggtgctctt taccccgact
    19261 ttctggcgtg aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg
    19321 gacaaacgcg cgagattgcc tcctacctgg cttcggaact gaaagaactg gggatccaga
    19381 cggatgtcgc caatgtgcac cgcattgaag aaccacaatg ggaaaattat gaccgtgtgg
    19441 tcattggtgc ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa
    19501 aacatgcgac gcgactgaat tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc
    19561 gcaaaccgga gaagcgtact ccacagacca acagctacgc gcggaagttt ctgatgaact
    19621 cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc
    19681 gctggtacga tcgttttatg atcaagctaa ttatgaagat gtcaggcggt gaaacggata
    19741 cgcgcaaaga agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg
    19801 cccagttaac caacaaaccg acgctgaaat aagcataaag aataaaaaat gcgcggtcag
    19861 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact
    19921 gacacggaac aacggcaaac acgccgccgg gtcagcgggg ttctcctgag
//
LOCUS       DAZSBF010000064        19872 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZSBF010000064 DAZSBF010000000
VERSION     DAZSBF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.065"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            35..1084
                     /locus_tag="ACHXWD_001791"
     CDS             35..1084
                     /locus_tag="ACHXWD_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR7620725.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1158..2015
                     /gene="nfo"
                     /locus_tag="ACHXWD_001792"
     CDS             1158..2015
                     /gene="nfo"
                     /locus_tag="ACHXWD_001792"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR7620726.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2019..3107
                     /locus_tag="ACHXWD_001793"
     CDS             2019..3107
                     /locus_tag="ACHXWD_001793"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR7620727.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3163..4413)
                     /locus_tag="ACHXWD_001794"
     CDS             complement(3163..4413)
                     /locus_tag="ACHXWD_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR7620728.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4513..5454)
                     /gene="rihB"
                     /locus_tag="ACHXWD_001795"
     CDS             complement(4513..5454)
                     /gene="rihB"
                     /locus_tag="ACHXWD_001795"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR7620729.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5584..6282
                     /locus_tag="ACHXWD_001796"
     CDS             5584..6282
                     /locus_tag="ACHXWD_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR7620730.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLLDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6349..7599)
                     /locus_tag="ACHXWD_001797"
     CDS             complement(6349..7599)
                     /locus_tag="ACHXWD_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR7620731.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7693..8631)
                     /gene="psuG"
                     /locus_tag="ACHXWD_001798"
     CDS             complement(7693..8631)
                     /gene="psuG"
                     /locus_tag="ACHXWD_001798"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR7620732.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8619..9560)
                     /locus_tag="ACHXWD_001799"
     CDS             complement(8619..9560)
                     /locus_tag="ACHXWD_001799"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR7620733.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(10018..11709)
                     /gene="fruA"
                     /locus_tag="ACHXWD_001800"
     CDS             complement(10018..11709)
                     /gene="fruA"
                     /locus_tag="ACHXWD_001800"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR7620734.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11726..12664)
                     /gene="fruK"
                     /locus_tag="ACHXWD_001801"
     CDS             complement(11726..12664)
                     /gene="fruK"
                     /locus_tag="ACHXWD_001801"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR7620735.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12664..13794)
                     /gene="fruB"
                     /locus_tag="ACHXWD_001802"
     CDS             complement(12664..13794)
                     /gene="fruB"
                     /locus_tag="ACHXWD_001802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR7620736.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14162..15343
                     /gene="setB"
                     /locus_tag="ACHXWD_001803"
     CDS             14162..15343
                     /gene="setB"
                     /locus_tag="ACHXWD_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR7620737.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15340..15594)
                     /locus_tag="ACHXWD_001804"
     CDS             complement(15340..15594)
                     /locus_tag="ACHXWD_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR7620738.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15749..16321
                     /gene="yeiP"
                     /locus_tag="ACHXWD_001805"
     CDS             15749..16321
                     /gene="yeiP"
                     /locus_tag="ACHXWD_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR7620739.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16544..18010
                     /locus_tag="ACHXWD_001806"
     CDS             16544..18010
                     /locus_tag="ACHXWD_001806"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR7620740.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18128..19114
                     /gene="yeiR"
                     /locus_tag="ACHXWD_001807"
     CDS             18128..19114
                     /gene="yeiR"
                     /locus_tag="ACHXWD_001807"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR7620741.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19153..19866
                     /gene="lpxT"
                     /locus_tag="ACHXWD_001808"
     CDS             19153..19866
                     /gene="lpxT"
                     /locus_tag="ACHXWD_001808"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR7620742.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
BASE COUNT         4927 a         5198 c         4790 g         4957 t
ORIGIN      
        1 gtcgtaacct tatgcccggt taaaggagag ggttatgacg aatatcacct tacagaaaca
       61 acatcgtaca ctgtggcatt ttattccggg gttagccctg agtgcagtta tcaccggggt
      121 cgccctgtgg ggtggttcca ttcccgcggt tgcgggtgcc gggtttagtg ccctcaccct
      181 cgcaatcttg ttggggatgg ttttaggcaa caccatctat ccgcacatct ggaaaagctg
      241 tgacggtggt gtgctgtttg ccaagcaata tttattacgt ctgggtatca ttctttatgg
      301 cttccgtctg acgttctcgc aaattgccga tgtcggtatc agtgggatca tcattgacgt
      361 gttgacgctg tccagtacct tcctgctggc ttgcttcctg gggcagaaag tgtttggtct
      421 ggataagcac accagctggt tgatcggtgc cggtagcagt atctgtggtg ctgccgcggt
      481 actggcgact gagtcggtag tgaaagcgga agccagtaaa gtaaccgtgg ctgttgcaac
      541 cgttgttatc ttcgggaccg tcgcgatttt cctctacccg gcgatatatc cgctgatgtc
      601 tcaatggttt agtccggaaa ctttcggtat ctacattggt tctactgtgc acgaagtggc
      661 gcaggtggtg gcggcaggtc atgccatcag cccggatgcg gaaaacgcag cagttatttc
      721 caaaatgctg cgcgtgatga tgctggctcc gttcctcatc ctgctggcgg cgcgtgttaa
      781 acagctgtct ggggcgaaca gcggcgagaa aagcaaaatc actattccgt ggtttgccat
      841 cttgttcatc gtcgttgcca tctttaactc gttccacctg ttaccgcaga gcgtggtgaa
      901 catgctggta acgctggata ccttcctgct ggcaatggcg atggcggcgc tgggtctgac
      961 cactcacgtc agcgcgctga aaaaagctgg ggcgaaacct ctgctgatgg cactggtact
     1021 gtttgcctgg ctgattgttg gtggtggtgc gattaactat gtgattcaaa gcgtcatcgc
     1081 ataaaccact acatcttgct cctgttaacc cgctatcatt accgttttcc tccagcgggt
     1141 ttaacaggag tcctcgcatg aaatacattg gagcgcacgt tagtgctgct ggcggtctgg
     1201 caaatgccgc aattcgcgcc gtcgaaatcg acgcaaccgc gtttgccttg ttcaccaaaa
     1261 accaacgtca gtggcgtgcc gcaccgctca cgacgcaaac catcgatgaa ttcaaagccg
     1321 cctgtgaaaa atatcactac acatcggcgc aaattcttcc ccacgacagt tatctgatta
     1381 acctcggaca tccggtcact gaagctctgg aaaaatcgcg cgatgccttt atagatgaaa
     1441 tgcagcgttg cgaacaactg gggctttctt tgctcaactt ccaccctggc agccatctga
     1501 tgcagatttc agaagaggat tgccttgcgc gtattgccga atccatcaac attgcgctgg
     1561 ataaaactca aggtgtaaca gcggtgattg aaaacaccgc cggtcagggc agtaacttag
     1621 ggtttaaatt cgaacatctc gcggcgatta tcgacggcgt ggaagataaa ttccgcgtcg
     1681 gcgtctgcat tgatacctgc catgctttcg ctgccgggta tgatttgcgt actccagccg
     1741 aatgcgaaaa aacgttcgct gattttgccc gtattgtcgg ctttaagtat ctgcgcggga
     1801 tgcaccttaa cgatgcgaaa agtacctttg gcagccgcgt tgaccgccat catagcctcg
     1861 gtgaaggcaa tatcggtcat gatgcgttcc gctggatcat gcaagacgac cgtttcgacg
     1921 gcattccgct gatccttgaa accatcaacc cggatatctg ggcagaagag atcgcctggc
     1981 tgaaagcgca acaaactgaa aaagcggtgg cctgatcgat gaacgacagg gaaaagcaga
     2041 ttcttaaaat tttacggcgt aacccgctga ttcaacagaa tgaaatcgcc gatattctgc
     2101 aaatcagccg ctcacgtgtt gccgctcata ttatggattt gatgcgcaag ggtttgatta
     2161 aaggcaaagg atatatcctt accgagcagg attactgtgt ggttgtgggt gcaatcaata
     2221 tggatattcg cggaatggcg gatatccgtt acccgcaagc agcctctcat ccaggttctg
     2281 ttcactgttc cgcaggcggc gtcggacgta atatcgcgca taacctggct ctgcttggtc
     2341 gggatgtgca tttaatatca gctattggca acgattttta cggcgagaca ttactggaag
     2401 aaacgcgccg cgcgggcgtg aacgtttcaa actgcattcg tctgcatgga cacagcactg
     2461 caacttatct cgctatcgct aataagcagg aagaaactat cctggcaatc aatgacacac
     2521 atattctgca acagctaacg cctcaactgc tcaatacttc acgggacttg attcgccatg
     2581 ctggtgtggt gctggctgat tgtaatctca cacctgaagc cctggagtgg gtctttacca
     2641 ttgctgatga aattccgatg tttgtcgata ccgtttccga atttaaagcg aacaaagtaa
     2701 aaaactggta cagccggatc cacactctga aaccaacaca aaatgaactg gaaattttgt
     2761 gggggcaacc gattaaagac gacaacgatc gcatcagagc tgtaaattcg ctgcatcaac
     2821 aaggtgttaa acgcattttt gtttatctga aagatgaatc tgtattttgc agcgataagg
     2881 atggagaaca atttttgctg accgcgccag cgcatacgac agtagacagt tttggtgctg
     2941 acgatggttt tatggcgggc ctggtatata gctttcttga aggatacagt ttccgcgaca
     3001 gcgcccgttt tgcggtagcc tgcgcggcaa tttcgcgcgc cagcggcagc ttaaacaacc
     3061 ctaccctgtc tgccgataac gcgctttcat tagtgccaat ggtgtaacaa tgttgccgga
     3121 ttcgacgctt gaccgcatct tatccggctc ataaattgcg cactacgcta aaccaataaa
     3181 gaatcctgca atagtagcac tcatcaggtt agaaagtgtc gccgccgcca gcgcgcgtaa
     3241 accaagttgg gcgatttccg gcgcacggtg tggcgcaacc gcagaaaacg cccccaccac
     3301 caccccgata gaaccaaagt tagcgaaacc gcacaacgcg aaagaaataa tcgccacggt
     3361 tttagcatcc agagtgccac ccgtttgcag atatggcgag aaattgagat aagcgacaaa
     3421 ttcattgatc gccagcttct gcccaatcaa actcccggca agatttgcat cactccagtc
     3481 aacccccatc acccacgcca atggggccaa caggtaacct aaaatcgact ccagcgaagc
     3541 atgttcaaaa ccaaaccagc cgccaacacc gccgataata ccgttaatca acgcaattat
     3601 tgcaacaaat gccataacaa ctgtcgctac accggcggcg attttcagcc cggtcattgc
     3661 ccctgtcgca gcggcttcaa taatgctttt tggcggtgtt tcggtgaaag agaggttatt
     3721 aaaagaaacc tgcgaagatt ccgtagccgg gcttaacagg cgggcaaaca agatcccgcc
     3781 agggatcgcc attaacgatg ccgccagcaa atattccaca ggtacgccca gtgcggcgta
     3841 accaatcatt gtcgaaccag caatcgaggc catgccacta caaatcgctg taaataattc
     3901 attgcgattc agacgatcga taaagggttt gacgatcgcc ggaatttcgt tttgcccgag
     3961 gaaaatggtg gtgaccgcga cgaatgactc gatcttgctg atatttaaag ctttctggaa
     4021 gataccgccg agaatgcgaa ttaagatccc catcacaccg atgtagtaga gaatactcac
     4081 cagtgcagtg acgaagataa ttgccggtaa taccctgaaa ccaaagataa atcctgcgcc
     4141 atcaaataag gtatccattt tcggtccgac cagagaaccg aagataaatg cgctacctgc
     4201 gtcgctgtac gccatcactt tgtggacgcc aaaagcgact ttttctgcga cccaacgccc
     4261 tggcggtaac caaagcataa tcccgccaat cacgacctgt aacactaacg ccgcgccaac
     4321 ggtacgcagg ctgatcttct tcttgtttac tgacagtaaa aacgcaattg ccagcagtac
     4381 caccattccc agaacacttc tcatgacatc catagtgatt atctcttcat gtcattttgg
     4441 gcgacggctt tctgcccatc agaaatagcc gggcctgcat cttgcgatac tgtcccggct
     4501 tgttatcaac acttaatggg tttttatgta gccgcgtacg cactcttcga ctaatcccca
     4561 gaaccagtcg gtatcaatag tgatgccgac tttagtattg gcgggcttgc caagaacgcc
     4621 cagctcgtcg cagacggtac gcccatagca agggccactg ttgacgtcca cttcgacgta
     4681 catctcctgg gttttaatgc catcagggtt aatcagataa ccgatgcagg tggcgtcgtg
     4741 caccgggccg ccagccaggc cgtagttttc gaactgcgtt ttgagagtga agttcatgat
     4801 gtcgctgaac atctctccgg cagggccgcc tgccctttcc attcgtgcaa ttacgtccgg
     4861 ggtgcaaacg gtctggttgg taagatcgag gcccatcatc actaatggaa cgccggaggt
     4921 gaacaccacg cgtgcggctt ccgggtcggc aaagatattg aactcggcag atggggtgaa
     4981 gttgcctgta ccgtaagcgc cgcccattag cacaatttca cggattttgg gcaggattgc
     5041 gggttgcata cgcattgcca ccgcgatatt tgaaagcgga ccaaccggca ccagagtgat
     5101 atcgccatcg cttgccataa gggtatcaat gatgtatttc accgcgtggg tactttctgc
     5161 ctggcgggtc agcggctcga ataccgggcc atccagtccg gtttcaccgt gaatattatc
     5221 ggcaacgatt tgttgacgca taatgggttg cggcatcccc gcataaaccg gaacattaat
     5281 ctccagtttc tggcaaacat tcaggccatt aattaatgtt ttatcaagcg tctgattacc
     5341 cgcaacaatg gtgataccta ataaatcaat cgccggatgt ttcgccgcca tcattatagc
     5401 aatagcatca tcatgacccg gatcacaatc cagaataatt tttctctttt ccattgttta
     5461 tttcctctgt ttccagttgc gttatttttt ctacagcaac gaaatttcga tcgggccgat
     5521 gattgaatct taacaacagc gtacgaatgc taaatatgag aaatctcata gcggataaac
     5581 atcgtgaaag aaatccacaa taatgatctt aagcagcaat tgatgagtga atccgcgttt
     5641 aaggattgct ttttaacgga tgtttcagcc gatacgcggc tgtttcattt tttagcgcgt
     5701 gactacattg tgcaggaagg gcaacagccg tcctggctgt tttacctgac gcgaggccgc
     5761 gccaggcttt acgccacgct agctaatggt cgcgtgtcgc tgctcgattt ctttgccgcc
     5821 ccctgtttta ttggcgagat tgagttaatc gacaaagacc atgaaccgcg tgcggtgcag
     5881 gctattgaag agtgttggtg ccttgcgctc cctatgaaac attaccgccc gctgttatta
     5941 aacgacacgc tatttttacg aaaactctgc gtcaccttaa gtcataaaaa ttatcgtaat
     6001 attgtttctt taactcagaa tcaatcattt ccgttagtga atcgcctggc agcatttata
     6061 ttactctcgc aggaaggtga tctttatcac gaaaagcata cgcaagcggc agagtattta
     6121 ggcgtttctt atcgacatct tttatatgtt ctcgcgcaat tcattcacga cggtttatta
     6181 attaaaagca agaaagggta tctaattaaa aacagaaagc agttgtcagg actggcgctg
     6241 gagatggacc cggagaataa attctccggg atgatgcagt aaaaattatt ggcaatagca
     6301 cgattgccgg atgcaacgct taataaggcg tacatccggc aatgaaactc aagctaaacc
     6361 aataaagaac ccggcaatgg tcgcgctcat caggttggaa agcgtcgccg ccgccagcgc
     6421 ccgtaaacca agctgggcga tttccggcgc gcgttttggc gaaatagccg aaaacgctcc
     6481 aacgacaaca ccgatagaac caaagtttgc aaacccacag agggcgaacg agataatggc
     6541 aatggtcttg acatccagcg tgccgctcgt ttgcagatag ggcgagaaat tgagataagc
     6601 aacaaactca ttaatcgcca gtttctgccc aatcaaactt ccggcaagat ttgcatcact
     6661 ccagtccacc cccataatcc atgccaacgg ggccagcaca tagccgaaaa tgctttccag
     6721 agagacattg gcaaaaccaa accagccgcc aactccacca ataatgccgt tgatcagcgc
     6781 aatgatcgct acaaatgcca ttaccaccgt cgccacaccg gcggcgattt tcagcccggt
     6841 cattgcaccg ttcgccgcag cttcaataat gctttttggc ggtgtttcgc taaacgacag
     6901 attttcaaac gtaacttgcg aaggttccgt cgccggacta agaatacgtg caaacaggat
     6961 accgccagga atagccatca gcgaagcggc taacaaatag tcaatcggca cccccattcc
     7021 ggcatagcca atcatcatcg aacccgcaat agacgccatg ccgctacaaa tagccgtaaa
     7081 tagctcgttg cgattcatgc gatcaataaa aggtttaacg atcgccggga tctcattttg
     7141 cccgaggaaa atagtggtga ctgcgacaaa agattcgatt ttgctgatgt tgagggcttt
     7201 ctgaaaaata ctgccaagaa tacgaatcag cagccccatt acgccaatgt agtacagcaa
     7261 gctgataagc gcggtaacga aaataatcgc cgggagtacg cgaaaggcga agataaagcc
     7321 cgcaccgtca aacagaacat ccattttggg accgaccagc gaaccaaaaa taaaggcact
     7381 tcctgcgtca ctgtaggaca tcactttatg gacgcctaat gccgcctgct ctactgccca
     7441 ttttcccggg ggaaaataga gcatgatgcc accaatggca atttgcagca gcagcgcagc
     7501 gccaaccgtg cgcaagctga tgctcttttt atttaccgac aacacaaagg caattgccag
     7561 caataccacc atccccgcaa cacttctcat tatatccata atgattttcc cttcatgcca
     7621 gtaaacccgg cgtcagcgcc aggttttggt atgtttgatg agtacgggcg acggctttct
     7681 gcccgccaga aattaaccca cgagacgctg atattctttg gcaatttcgc tcgccagaat
     7741 ggcgttatta aagaccagtt gaatattgga tttcaggcta tcaccgccgg ttagctccgc
     7801 aacacgcgcc agcaggaacg gtgtactctc tttcccaata acaccctgtt cttcggcttc
     7861 agcaacggct tgatcaattg ctgcattgat ggtttcttct ggcatcgcaa actgttccgg
     7921 gatcgggttc gctaccacca ggccgccgtt caaaccggtt tgccacttca ctgccatcgc
     7981 gcgagcaatt tcgcgggcac tgtcaagacg aatgctgact tcaaatgggc tggttcggca
     8041 gaaaaacgca ggcagcgctt ttgtctgata accaattaac ggtacaccga aggtttctaa
     8101 atattctgtt gttaatccta aatcgagaat agattttgcc cccgcacaaa caacagtgac
     8161 attggtattt gccagttcct gtaaatcggc agaaatatcg aaggtatgtt ccgctccgcg
     8221 atgcacacca ccaatacctc cggttgcaaa aactttaatc ccagccagtg ctgcaataat
     8281 catggttgat gcaacggtag tcgcgccatt tttccctgca gcaacgacaa aaggtaaatc
     8341 acgacgacta actttcgtca cgttatgtcc ttcacggccc agcaattcga tttcttcttt
     8401 acttaagccc actttcatca caccgccaat aatggcgata gtggctggta ctgcgccctg
     8461 tttacgaatt gtttcttcta cttcaattgc ggtttgggca ttttgtggga atggcattcc
     8521 gtgagaaatt atggtcgatt ccagcgcaac aaccggtttt ttatttttta aagcatcctg
     8581 tacttcagcg gatatttgta ataattcagg ggacagagtt aattcagaca ttctgtgttc
     8641 tccactaacg atctaacatt ggcaattgat aattcagggt tgttggtata ttcacaggca
     8701 agtgccattg acgaacatcc ctgcgcaaat cgaacagaat cgataaacgg cataccgtct
     8761 acccaacagg aggccagccc cgccatcatg gcgtcgcctg ccccagtaac attgatgaca
     8821 ttcgttttaa taggggccga ccacccactt tcaccgttga tatcactgta gtaaacgcca
     8881 tcccctccca tgctcagcac caagcggttc aggccgtgtt gatgaaacca ggcggcgacc
     8941 tttgcgacat cttcacgtcc ggaaagtgca attccgctca acgtttcggc ttcaagacgg
     9001 ttaggtttta acgtatggat tttgctcaga tgatcgcgga ttttgacgca tttccatgcc
     9061 gataccggat caataaatac aggcgtttcc ccgctatttt ccagaaccca gaccagcacc
     9121 tcttcactaa ggttgcaatc cgcgacaata acctttgcac cgcgaataaa ctcctgatgc
     9181 tgggcaagaa attctgcaga aatacagtca ctaatactca tatcatttat cgcgacaagc
     9241 atttctcctg tattatcgag cagtgataaa tagctggatg tattctcacc cggaacaatt
     9301 aagcatttat cgacataaac accggattga ttagtttgcg ccagtaatga ttgcccataa
     9361 aaatcaccac ctactgcact taaaagccag gcatttttcc ccagtaacgc cagattatga
     9421 gcgatattcc ttcccacgcc acctggggta aatttaattt ttccgggatt cgaatccgca
     9481 taattcaatg gatgatgtga atacccagcc acatcaatat tcgccgaacc aataattact
     9541 atgtagtcct tttcgcgcat agcacgcccc ctggcaataa attgccaacg aagaaattgg
     9601 gtaaaatagc cattacagtc aataagacta ttagagcacg tgttcagatt taaacacatg
     9661 ttcataataa gcatatttaa cgtaatgtaa atcgatcaga aaaaacgaaa tcggccagat
     9721 atgaaggcgg tcacaaagtt tgtctattta tgcagcaaca aaagagaaag ttgtgatgtg
     9781 taacacacaa tatggcaggc aagaaaaagg gcagagatgt ttctgccctc cagattgttg
     9841 acaacgcgcg ctttgttcat cctggatgtg gcgtaaacgc cttatccgac ctacaaaagc
     9901 gcaaaaaatt caatatattg cagggttcat gtaggtctga taagcatagc gcatcaggca
     9961 attttgcgtt tgtcatcatc ggggcagaga tgtctcatcc ccgataatga agataaatta
    10021 tgcgtttttt tctactattt gtgtttccgg gcgtttcagg aaggcatagg ccaaacccgc
    10081 caccagcgta ccggcaataa ttgctaccag gtaacccaat accggcgtaa tagcgccagg
    10141 gatcagcaga acaaacagac caccgtgtgg tgccatcagt ttcgcaccaa tcgccattga
    10201 gattgcgcca gtcagcgccc caccaacgat acagcacggt agcacacgca tcggatcacg
    10261 agcagcaaac ggaattgcac cttccgaaat aaagcacaga ccaagtacca gagcggcttt
    10321 gccaccttcc tgctgcgctt tgtcgaattt gcgacgcgcc accattgttg ccagacccat
    10381 tgccagcggt ggcaccatac ctgccgccat aatcgccgcc atcgggccat aggtttgagt
    10441 actcagcaga cccacaccga atgcgtacgc tgctttgttt accggaccgc ccatgtcagt
    10501 acacatcatg ccaccgagga tcgcccccag cagaaccgca ttcgcagtcc ccatggtctg
    10561 cagccagtga gtcaacccat cgagaatgcc agcaaccggt ttaccgatta ggtagatcat
    10621 cgccagaccg accaccagac tggaaattag cggaatgatc aggatcggtt tcagcgcctc
    10681 catactctgt ggcagtttca attgcgtact gattaacttc gcaatgtaac cagccaggaa
    10741 gccagcaatt ataccgccaa taaagccaga accggtgctg accgccagca taccgccaat
    10801 cagacccggt gtgaggcccg gacgatcggc aatggagaag gcaatataac ctgccagtac
    10861 cggcaccatc agcgcaaagg ctgaaccacc accaatctgc atcagcgccg ctgccaacgt
    10921 acccggctct ttaaacgctt cgataccaaa agcaaaagaa agcgcgatac acagaccacc
    10981 tgcaacaacc atcggcagca tgtaagagac gcccgtcagc aagtgacggt aagcgcctgc
    11041 actctctttc ttaccttcag tggtcgctgt ttgagctttg cccgctggtt catacggcgt
    11101 tgcttcagca accgctttat ccagttcctg cgcagttttc ttcagcgcca gaccggtaga
    11161 ggtgcgatac atcggtttac cagcaaattt cgccagatcc acttcgatat ctgccgccac
    11221 aatcaccaga tccgctgctg cgacttcttc aggggtgatt gcattgcccg cgccaacaga
    11281 accacgggtt tcaactttca cccaccagcc acgttttttc gcttcggttt caatggcttc
    11341 agccgccata aaggtgtgtg ctacgccagt cgggcaagca gtcaccgcaa ctacgcgttt
    11401 cggaccgctg gcagcaaccg gtgctgtcgc agcgaccggc gcagtgtaag gtttcgcatg
    11461 gcctttggct tcactcagga acagctcagg gtgcgcaact gcccgggaaa tatcacccag
    11521 ccagacattt ttaccgttca gcgcgctgtc attcgggatg gaatcaccga gaacaatcgc
    11581 catttctgcg tcgttcggat tgtcgatgat ttccagtttt gcttttcgcg ccgccgcgcc
    11641 cagcagggtc ttcgccatat aggcgcgtgc ctgaccgaga ttagcgtcaa taatcagcag
    11701 cgttttcatt atgcctctcc tgctgtcagt taaaaggttg taagtcgacg cgcgccatca
    11761 ttgcggccaa ctgcggacga tcggtaatac ccacattgct ttgacttacc gccagggctg
    11821 caacagctgt cgccagacgc agtgtgtgtt cactggattc acgcatcagc aagccataaa
    11881 tcaggccacc aaccatagaa tcccctgcgc caacggtgct tacgacatcg actgacggtg
    11941 gtttggcgat ccattcgccg gaggcattaa cccaaagcgc gccttcggca cccagtgaaa
    12001 taacaacatg cgcaatacct tgttcacgta gcgcatgtgc agcttcaatc acatctttca
    12061 tttcaggcag tttacggcct gcccagattt ccaactcgcg gcggttaggt ttcaccagcc
    12121 acggtgccgc tttcaaacct gctactaacg cttcacggct actatcaaag ataatgcaag
    12181 gacactgact acgcaggcga gtcatccagt cggtgaacgc ttccgggctg acgcctgacg
    12241 gtaagcttcc gctgacacag accatatcga actgaccgag ccagctcaga gaatcagtca
    12301 caaagcgttc ccagtcggcg agggtgactt caaaacccga gaagttgaag tcggtcactt
    12361 cgccgtcttt ttccgtcagc ttaacgttaa ttcgggtgcg cccctgtaca acctggaaac
    12421 ggttggcaat gcccagctcg ctgaacagtt gctgaaaacc atcctgattg tctttaccca
    12481 ggaagccgcc aacggtgaca tcaattccca ggtcttttaa tactttggcc acgttgatgc
    12541 ctttacccgc cgcatgcaga ccggtggttt tcaccaggtt cacttcgccg cgttcaattt
    12601 ccgggcagaa accaacaagg tcataagccg gattaagggt gatcgtagca acacgtctgc
    12661 tcattatgcg ccctccccaa gaccagcagc gatagcgtcg ccaatggctt tcagcgcctg
    12721 ttcagcatct gcaccctggg cggtaaagcg taggcgatga cctttcttaa cgccaagtgc
    12781 cacaactttc atcagactac gtccgtttgc cggtttgccg gtgccatcaa ggtttgtcac
    12841 ggtaatatca ctgttaaatt gtttaatggt attgaccagc atggtacctg gacgagcatg
    12901 caggccgtgt tcattgcgca ccacaaactc cgcgcttaac acgtcgtcgg tcggcgcatc
    12961 atcgctggtc agcagcgcca gcaacgttgc cgcatccgct ttcagcaagc ggtcagcttt
    13021 attgtcgagc aataaatcag cgagacgctt aagaaccgcg atgggctgat cgtcattcat
    13081 cgccacactc accagcatgg ctgccgtttc gccgtccaca tcaaaagcat ttgccgcacg
    13141 gcttaccgca atcgcgctac gcagattgcc ttcggcgcta tcgctcagcc agataccctg
    13201 tccgagattc agcggttgtt cattgatggc tttggtgacg aaagtggcgt caactgcccc
    13261 cacctctttc agacgcgcag cgttcagcgc ctgaagagtc agcagatcgc tggcgacgat
    13321 atccagcgtc agcatttcgt tgtcgagctt cagctgctca ctctgctttt cgcccatcag
    13381 taatgcgcga agttcttctg ctgttgttgc tgacttcagt tgttcagcaa cggaatcatc
    13441 gctcagtacg tgggtcagct ggcgtagcag gcccagatgt tcatccgagc tggcagcaat
    13501 accgattgcc acgtacgcta cctgaccgtc accccaggtg acgccttccg ggaactgaaa
    13561 tacctgaacg ccggttttca gcacctgatc gcgggtgtcg gtagtgccgt gtggaatagc
    13621 aataccattg ccgaggaacg ttgaggtttg ctgttcgcgc gccagcatgc cattgacgta
    13681 gccttctgct acattaccgg cctgcaccag cgccgcagcg acctggcgaa tcgcctcttc
    13741 tttgtctccg gccttttcgc ccggatggat gtcctgtacg gataactgga acatagttct
    13801 cctctcttgc tgaattgaaa cgattcagcc tctatgagaa aaaaagcgcc aacctggctt
    13861 agggttaaag acaagatcgc gctgaaacgt ttcaagaaag cataatactt ctgtttcagc
    13921 acgcaaggaa agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgtca
    13981 tttttcctaa ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc
    14041 agccaatcag caacagtgaa atggcatttt gattattcgt ggcgcttttg atttcgctgt
    14101 ctgtttattt tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt gatcgacctg
    14161 catgcataac tcccccgcag tctccagcgc gaaatcgttt gacctgacct cgacggcgtt
    14221 tttaatcgtt gcctttctca ccggtattgc gggtgctctg caaaccccga cactcagtat
    14281 tttccttacc gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag
    14341 cgctgtcatt gggattctgg taagtcagtt tctcgctggg cgctctgata agcgcggcga
    14401 tcgcaaatcg ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc
    14461 ctggaatcgc aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc
    14521 gaccgctaac ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga
    14581 ggcggtgatg ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc
    14641 accgctggct tatgcgttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc
    14701 ggtagcgttt attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga
    14761 gcttccgctg gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg atacgctact
    14821 gctgtttgtc atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc
    14881 gctatttatt atcaacgaac tgcatcttcc cgagaaactg gccgatgtga tgatggggac
    14941 cgccgccggg ctggaaatcc cgacgatgtt gattgccgga tatttcgcca aacgtctggg
    15001 taagcgtttc ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct
    15061 gatggcgcat tcacctgtca ttctgttggg cttgcagctg ctaaatgcta tttttattgg
    15121 cattctgggc ggcatcggga tgctctattt tcaggatctg atgcccggtc aggcgggttc
    15181 agccaccacg ctctatacca acacttcgcg cgtgggctgg atcatcgcag gatcagtggc
    15241 gggcatcgtc gccgaaatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat
    15301 tatcgccact ctgttttgct tactgcggat taaagatgtt taaggcgcgg tcagcatttc
    15361 cagatcgata agccaggtca tggcctgctg acgcgagtta ccgcacattt cagcgctggc
    15421 ttgtaagcct gcacagactt tcggtcgcag tggcgaggtg aaaattttac agcgttgctg
    15481 ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg
    15541 gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg
    15601 ttctcttaag gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag
    15661 caaaagtgcg aataccactt gcccgaaagg cccgtcgcga gtactttgtc gcgatatttt
    15721 tgacattttc gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat
    15781 ggtactgaat tacaacggca aactgctgct ggttaaggat attgatattc agtcgcccac
    15841 tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa
    15901 agtagaagag cgtttcaaag gtgatgatat cgttgacacc gtgacgctga cccgccgtta
    15961 cgttgacttc tcctatgtcg atggcaacga atatgtcttt atggataaag aagactatac
    16021 cccgtatacc ttcaccaaag atcagattga agaagagttg ctgtttatgc cagagggcgg
    16081 catgccggac atgcaggtgc tgacctggga cggtcaactg ctggcgcttg agctgccgca
    16141 gaccgttgat ctggaaatcg ttgaaaccgc accaggcatc aaaggggcat ctgccagcgc
    16201 ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag
    16261 cccgggcgaa aaaattcgta tccatatcga agaacgccgt tatatggggc gtgctgacta
    16321 acttcagccg catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc
    16381 aatcttctat tttgcgcttc ctgaacacct tacgccctca attttcactc gttgattgat
    16441 cgccctcaca cttcatcgca ttaacaatcc agaccaattt caattgctgt catataactt
    16501 tacactgtca ttgttaattt atcgttacta agacgtgact cctatgaaga caattgcctc
    16561 cgttacgctc ccgcatcatg tacacgctcc acgctacgat cgccagcagt tgcaatcacg
    16621 tatcgttcat tttggctttg gcgcctttca ccgcgctcat caggcgttac tgaccgatcg
    16681 tgtgctgaat gcccagggcg gcgactgggg gatctgtgaa atcagcttgt tcagcggtga
    16741 tcaactgatg agccagctcc gcgcacagaa ccatttatat accgtgctgg agaaaggtgc
    16801 ggacggcaat caggtgataa ttgtcggtgc cgttcacgaa tgccttaatg caaaactgga
    16861 ttccttagcg gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac
    16921 gattaccgaa aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagtaatcc
    16981 gcggattatt cacgatctac aaacccctga agaacctcac tccgcaccgg gtattctcgt
    17041 cgaagcactg aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga
    17101 caatattccc gacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaatg
    17161 ttcgccagaa ctcgccgggt ggataaaaaa acacgtcagt tttccgggaa ccatggtcga
    17221 ccgcattgtt ccggctgcaa ccgacgaatc actggcagaa atcagccagc atctgggggt
    17281 aaatgatccc tgcgcgatta gctgcgaacc gtttatccag tgggtggttg aagataactt
    17341 catcgctggg cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc
    17401 atgggaagag atgaaactgc gtatgcttaa tggcagccac tcttttctcg cttatctggg
    17461 ttacctctca ggattcgtcc atatcagtga ttgcatgcag gatcgcgtat ttcgccatgc
    17521 tgccagaaca ttaatgctgg atgagcaagc gccgacactg caaattaaag atgtcgattt
    17581 aacacaatat gcggataagt taattgcacg ttttgctaat ccgtcgctga aacataagac
    17641 ctggcaaatc gcgatggatg gcagccagaa attaccgcaa cgtatgctgg caggtattcg
    17701 catacatctg gggcgtgaaa cggactggtc gttgctggca ttaggcgttg ctggctggat
    17761 gcgttacgtc agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag
    17821 cgataaaatt cgcgaacttg ttgcgggcag cagcagtgaa caacgcgtaa ccgccctgct
    17881 ttccctgcgt gaagttttcg gtgatgatct gccagataac ccgcattttg tgcaggccat
    17941 cgaacaagcc tggcaacaaa tcgtacaatt cggcgcacat caggcgctat taaacaccct
    18001 caaaatttaa cgatttctgc ggttaaagcg gatgaagctc accttcgtcc gctctcccct
    18061 tctcttttct gcctttttta gccaggatta acgctcagtt aacttaccag agtattgcgg
    18121 agccgttgtg accaggacca acctcatcac cggttttctc ggcagcggga aaaccacgtc
    18181 gattcttcat ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga
    18241 atttggggaa gtcggaattg atggtgcttt gctcgccgat agcggcgcat tgctgaaaga
    18301 gatccccggc ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac
    18361 cttactgcgt cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc
    18421 gaaacagatc ctcgatctgt taaccgcacc agtctatgaa ctgtggatag atctgcgcgc
    18481 caccttgtgc attctcgatc cacgcctgct gctggacgaa aaaagcgcca gcaatgaaaa
    18541 cttccgtgac cagctggctg ccgcagacat cattgtcgcc aataaatccg accgtgcgac
    18601 gcccgaaagt gagcaagcgc tacagcgttg gtggcagcaa aatggtggcg atcgacaatt
    18661 aattcacagt gagcatggga aagttgacgg tcatcttctg gatttgccgc gtcgcaattt
    18721 agccgagttg cccgccagcg ccgcgcattc tcatcagcat gtcgtgaaaa aagggttagc
    18781 agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg ggcaaggata
    18841 tcaggcctgc ggctggatat tcgacgctga tacggtattc gacaccattg gcattctgga
    18901 atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct
    18961 ggtacgaatc aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc
    19021 ggacagccgt attgagctga tttccagcag cgaagctgac tggaatgctt tgcagagcgc
    19081 gctgttgaag cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata
    19141 attagagaaa atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc
    19201 gcgctgtttc tttcctggta tattcccgtt aatcatggat tctggttgcc gattgatgcg
    19261 gatatttttt atttctttaa tcagaaactg gtcgaaagta aggccttttt gtggctggtt
    19321 gcattgacca acaatcgcgc tttcgacggt tgttctctgc tggcgatggg aatgttgatg
    19381 ctgagtttct ggctgaaaga aaacgcccct ggcagacgac gtatcgtgat tatgggtctg
    19441 gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcattaat tcctgtaaaa
    19501 cgggccagcc caacattgac ttttaccgat attaaccgcg tcagcgaact gctctctgtt
    19561 cccacgaaag atgcctcacg agatagcttt cccggcgatc acggcatgat gctgcttatt
    19621 ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc ccttattatt
    19681 tttgtggttt ttgcatttcc cagagtaatg attggcgcac actggtttac tgacatcatt
    19741 gtcggttcga tgaccgtgat attgatcggt ttgccctggg tgttgctgac gccattaagt
    19801 gatcgattaa tcaccttttt tgacaaatca ctaccgggaa aaaacaaaca tttccaaaac
    19861 aaataactca ca
//
LOCUS       DAZSBF010000065        19377 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZSBF010000065 DAZSBF010000000
VERSION     DAZSBF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.066"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            198..274
                     /locus_tag="ACHXWD_001809"
     tRNA            198..274
                     /locus_tag="ACHXWD_001809"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:232..234,aa:Arg,seq:acg)
     gene            555..1121
                     /gene="yqaB"
                     /locus_tag="ACHXWD_001810"
     CDS             555..1121
                     /gene="yqaB"
                     /locus_tag="ACHXWD_001810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR7620743.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            1118..1546
                     /locus_tag="ACHXWD_001811"
     CDS             1118..1546
                     /locus_tag="ACHXWD_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR7620744.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1619..3175
                     /gene="gshA"
                     /locus_tag="ACHXWD_001812"
     CDS             1619..3175
                     /gene="gshA"
                     /locus_tag="ACHXWD_001812"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR7620745.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            3325..3840
                     /gene="luxS"
                     /locus_tag="ACHXWD_001813"
     CDS             3325..3840
                     /gene="luxS"
                     /locus_tag="ACHXWD_001813"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR7620746.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3904..5442)
                     /gene="emrB"
                     /locus_tag="ACHXWD_001814"
     CDS             complement(3904..5442)
                     /gene="emrB"
                     /locus_tag="ACHXWD_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR7620747.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5459..6631)
                     /gene="emrA"
                     /locus_tag="ACHXWD_001815"
     CDS             complement(5459..6631)
                     /gene="emrA"
                     /locus_tag="ACHXWD_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR7620748.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6758..7288)
                     /gene="emrR"
                     /locus_tag="ACHXWD_001816"
     CDS             complement(6758..7288)
                     /gene="emrR"
                     /locus_tag="ACHXWD_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR7620749.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7379..7714)
                     /gene="ygaH"
                     /locus_tag="ACHXWD_001817"
     CDS             complement(7379..7714)
                     /gene="ygaH"
                     /locus_tag="ACHXWD_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR7620750.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7704..8441)
                     /gene="ygaZ"
                     /locus_tag="ACHXWD_001818"
     CDS             complement(7704..8441)
                     /gene="ygaZ"
                     /locus_tag="ACHXWD_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR7620751.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8565..9749)
                     /locus_tag="ACHXWD_001819"
     CDS             complement(8565..9749)
                     /locus_tag="ACHXWD_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7620752.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(10040..11032)
                     /gene="proX"
                     /locus_tag="ACHXWD_001820"
     CDS             complement(10040..11032)
                     /gene="proX"
                     /locus_tag="ACHXWD_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR7620753.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(11090..12154)
                     /gene="proW"
                     /locus_tag="ACHXWD_001821"
     CDS             complement(11090..12154)
                     /gene="proW"
                     /locus_tag="ACHXWD_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR7620754.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(12147..13349)
                     /gene="proV"
                     /locus_tag="ACHXWD_001822"
     CDS             complement(12147..13349)
                     /gene="proV"
                     /locus_tag="ACHXWD_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR7620755.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13705..14664)
                     /gene="nrdF"
                     /locus_tag="ACHXWD_001823"
     CDS             complement(13705..14664)
                     /gene="nrdF"
                     /locus_tag="ACHXWD_001823"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR7620756.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14674..16818)
                     /gene="nrdE"
                     /locus_tag="ACHXWD_001824"
     CDS             complement(14674..16818)
                     /gene="nrdE"
                     /locus_tag="ACHXWD_001824"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR7620757.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTNEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16791..17201)
                     /gene="nrdI"
                     /locus_tag="ACHXWD_001825"
                     /pseudo
     CDS             complement(16791..17201)
                     /gene="nrdI"
                     /locus_tag="ACHXWD_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(17198..17443)
                     /gene="nrdH"
                     /locus_tag="ACHXWD_001826"
     CDS             complement(17198..17443)
                     /gene="nrdH"
                     /locus_tag="ACHXWD_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR7620758.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17691..18020)
                     /locus_tag="ACHXWD_001827"
     CDS             complement(17691..18020)
                     /locus_tag="ACHXWD_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR7620759.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            18172..18516
                     /locus_tag="ACHXWD_001828"
     CDS             18172..18516
                     /locus_tag="ACHXWD_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR7620760.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18553..19002)
                     /gene="alaE"
                     /locus_tag="ACHXWD_001829"
     CDS             complement(18553..19002)
                     /gene="alaE"
                     /locus_tag="ACHXWD_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR7620761.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT         4869 a         4883 c         5005 g         4620 t
ORIGIN      
        1 attctccgaa atcttcagca atgaaggtat cgaagaggta gcggaattaa ccgcaggcac
       61 cagggatgat aacgttgctt tagcaacggc ccgaagggcg agccgcaagg cgagtaatcc
      121 tcccggatgc accatctctt aattgatatg gctttagtag cggtatcaat atcagcagta
      181 gaataagttt tcccgatgca tccgtagctc agctggatag agtactcggc tacgaaccga
      241 gcggtcggag gttcgaatcc tcccggatgc accatattct ccgtaacctt cagcaatgaa
      301 ggtatcgaag agatagcggt attaaccgca ggcaccagga aggataacgt tgcgccagca
      361 acggcccgaa gggcgaacgt cagtgagtca tcctcccgga tgcaccatct tctccaaaaa
      421 atttccttca aaaatatctc atttctaaat aactccttta tccatttcct gaattttctt
      481 ctccttcgcc atcagcgaca aaatcaagca attacgctaa agtagcgtca tatttttcga
      541 cttgcgagaa gaccatgtac gagcgttatg caggtttaat ttttgatatg gatggcacaa
      601 tcctggatac ggagcctacg caccgtaaag cgtggcgcga agtattaggg cactacggtc
      661 ttcagtacga tattcaggcg atgattgcgc ttaatggatc gcccacctgg cgtattgctc
      721 aggcaattat tgagctgaat caggccgatc tcgacccgca tgcgttagcg cgtgaaaaaa
      781 cagaagcagt aagaagtatg ctgctggata gcgtcgaacc gcttcctctt gttgatgtgg
      841 tgaaaagttg gcatggtcgt cgcccaatgg ctgtaggaac ggggagtgaa agcgccatcg
      901 ctgaggcatt gctggcgcac ctgggattac gccattattt tgacgccgtc gtcgctgccg
      961 atcacgtcaa acaccataaa cccgcgccag acacattttt gttgtgcgcg cagcgtatgg
     1021 gcgtgcaacc gacgcagtgt gtggtctttg aagatgccga tttcggtatt caggcggccc
     1081 gtgccgcagg tatggacgct gtggacgttc gcttgctgtg agtgaagcgt tatcgctttt
     1141 ctcgttgttt gccagtagtt ttctcagcgc tacactatta cccggcaact ccgaagtcgt
     1201 gctggtggca atgttgcttt ccgggatcag tcatccctgg gttttagttt taacagcaac
     1261 aatgggtaat agccttggag ggttaactaa cgttatcctt gggcgtttct ttccattgcg
     1321 taaaacatcg cgctggcaag agaaagctat cggctggctg aaacgctatg gtgcagtcac
     1381 gctattatta agctggatgc ccgtggttgg cgatttactg tgtctgttag cgggatggat
     1441 gcgcatctcg tggggaccgg taatcttttt tttgtgcctt ggtaaagcgt tacgctatgt
     1501 tgcagttgca gcagcgaccg ttcagggcat gatgtggtgg cactaattgt aggcctgcac
     1561 atatggtcac cattacagtt atgctaatta aaacgatttt gacaggcggg aggtcaattt
     1621 gatcccggac gtatcacagg cgctggcctg gctggaaaaa catcctcagg cgttaaaggg
     1681 gatacagcgt gggctggagc gcgaaacttt gcgtgttaat gctgatggca cactggcaac
     1741 aacaggtcat cctgaagcat taggttccgc actgacgcac aaatggatta ctaccgattt
     1801 tgcggaagca ttgctggaat tcattacacc agtggatggt gatattgaac atatgctgac
     1861 ctttatgcgc gatctgcatc gttatacggc gcgcaatatg ggcgatgagc ggatgtggcc
     1921 gttaagtatg ccatgctaca tcgcagaagg tcaggacatc gaactggcac agtacggcac
     1981 ttctaacacc ggacgcttta aaacgctgta tcgtgaaggg ctgaaaaatc gctacggcgc
     2041 gctgatgcaa accatttccg gcgtgcacta caatttctct ttgccaatgg cattctggca
     2101 agcgaagtgc ggtgatatct cgggcgctga tgccaaagag aaaatttctg cgggctattt
     2161 ccgcgttatc cgcaattact atcgtttcgg ttgggtcatt ccttatctgt ttggtgcatc
     2221 tccggcgatt tgttcttctt tcctgcaagg aaaaccaacg tcgctgccgt ttgagaaaac
     2281 cgagtgcggt atgtattacc tgccgtatgc gacctctctt cgtttgagcg atctcggcta
     2341 taccaataaa tcgcaaagca atcttggtat taccttcaac gatctttacg agtacgtagc
     2401 gggccttaaa caggcaatca aaacgccatc ggaagagtac gcgaagattg gtattgagaa
     2461 agacggtaag aggctgcaaa tcaatagcaa cgtgctgcag attgaaaacg aactgtatgc
     2521 gccgattcgt ccaaaacgcg ttacccgcag cggcgagtca ccttctgatg cgctgttacg
     2581 tggcggcatt gaatatattg aagtgcgttc gctggacatc aacccgttct cgccgattgg
     2641 tgtagatgaa cagcaggtgc gattcctcga cctgtttatg gtctggtgtg cgctggctga
     2701 tgcaccggaa atgagcagta gcgaacttgc ctgtacgcgc gttaactgga accgggtgat
     2761 ccttgaaggt cgcaaaccgg gcctgacgct gggtatcggc tgcgaaaccg cgcagttccc
     2821 gttactgcag gtgggtaaag atctgttccg cgatctgaaa cgcgtcgcgc aaacgctgga
     2881 tagcattaac ggcggcgacg cgtatcagaa agtgtgtgat gaactggttg cctgcttcga
     2941 taatcccgat ctgactttct ctgcccgtat cttaaggtct atgattgata ctggtattgg
     3001 cggaacaggc aaagcgtttg cagaagccta ccgtaatctg ctgcgtgaag agccgctgga
     3061 aattctgcgc gaagaggatt ttgtagccga gcgcgaggca tctgaacgcc gtcagcagga
     3121 aatggaaacc gctgataccg aaccgtttgc ggtgtggctg gaaaaacaca cctgacagaa
     3181 aagaaaaagg ccactcgtga gtggccaaaa tttcatctct gaattcaggg atgatgataa
     3241 caaatgcgcg tctttcatat actcagactc gcctgggaag aaagagttca gaaaattttt
     3301 aaaaaaatta ccggaggtgg ctaaatgccg ttgttagata gcttcacagt cgatcatacc
     3361 cggatggaag cgcctgcagt tcgggtggcg aaaacaatga acaccccgca tggcgacgca
     3421 atcaccgtgt tcgatctgcg cttctgcgtg ccgaacaaag aagtgatgcc agaaagaggg
     3481 atccataccc tggagcacct gtttgctggt tttatgcgta accatcttaa cggtaatggt
     3541 gtagagatta tcgatatctc gccaatgggc tgccgcaccg gtttttatat gagtctgatt
     3601 ggtacgccag atgagcagcg tgttgctgat gcctggaaag cggcaatgga agacgtgctg
     3661 aaagtgcagg atcagaatca gatcccggaa ctgaacgtct accagtgtgg cacttaccag
     3721 atgcactcgt tgcaggaagc gcaggatatt gcgcgtagca ttctggaacg tgacgtacgc
     3781 atcaacagca acgaagaact ggcactgccg aaagagaagt tgcaggaact gcacatctag
     3841 tcagtaaact atcttcacaa ttaattgaaa aaagccagtt caaatgaact ggcttagttg
     3901 tacttagtgc gcaccgccgc caccgccacc tgcgccaaat ggcggtttag caaaccacac
     3961 cagccccagc agtacgagga atatcccggc tgacatccag aagatctcat tggcggaaat
     4021 aatcagcccc tgattggtga tctgctgggc aatccagcct gatgcctgct gttgcgtcat
     4081 cccaagccct tccagttgac tgtacatcgc ctgggcattc gggttgaacg ggtttaccga
     4141 ctcagtcaac tgcgcatggt gcatcgactc gcggttggtc cacatggtcg tggttatcga
     4201 cgtgccgata gaccccgcca gcgttcgcgt aaagttagag aggctcgatg ccgccgccag
     4261 tcgttccggt ggcaaaccag acagcgtaat ggtggtcagc ggcataaaga agcaggccac
     4321 cgcaaacccc tggataaact gcggccaggc cgacgcgcca aaatccatac ctggttcaaa
     4381 ggtataggca cgccagtaga agcagacggc atacataata aagctgaagg ttaccagccg
     4441 ccgcatatcc agtttatgcg cgaagcggcc gataatcggc gacaggatca ccggaataat
     4501 ccctaccggc gcagaggcca aacctgccca ggtcgccgtg taaccgtaga cctcctgcaa
     4561 caactgcggc agcagaacaa tagcgccgaa gtagagcata tacgcgaggc tgatacacaa
     4621 gcagccgatg gtgaagttgc gcgacttaaa caacgacaga tcgactatcg ggttatcgtc
     4681 ggtcagctcc cagacaatca ggaagcagat agccaccacc gccaccacgg taaggatgat
     4741 aatttcctgt gatgaaaacc agtccagctc tttaccgcgg tcgagcataa tctgcaggct
     4801 gccgatacca ataaccagca gtgccagccc cacggcatca atccgccgcc gtttggtgcg
     4861 ggtttcacgt ccgcgcagag tttgcagtgt catcaacacc accgccacgc caatcggcac
     4921 gttgatgaag aatatccagc cccagtggta attatcgctg atataaccgc cgaggatcgg
     4981 gccgcaaatt ggcgcgacaa tcaccgtcat cgaccacaac gccagcgcga tcgagcgttt
     5041 ggctggcggg tagttattca gcaatagact ttgcgaaagc gggatcaacg gcccggcgac
     5101 aatcccctga atcacgcgga agaagatcag catattcagg ctgctggaga caccacacgc
     5161 ccacgacgca atagcaaagg cgatggtgga ccaaaggaac agtttcactt ccccgacgcg
     5221 ctttgccagc cagccggtaa gcgggatcga gatggcattc gccaccccga aagaagtgat
     5281 tacccacgtt ccctggctga gcgatgagcc cagattcccg gcgatagtgg ggatcgccac
     5341 gttagcaatg gtggagtcca gcacctgcat gaatgtcgcc agtgacagcg caatcgtcat
     5401 aatgaccagt tgcgcgcctt ccagcggttt ttgctgttgc atcacacgca cctctggatt
     5461 agccagcgtt agcttttacg atatcgtcga tcagtttatt gacaggtgcc aggctgattt
     5521 cacgcgcggt acttaccgcg accggtgttg aacgtacttt atttgccagt acctgaccgt
     5581 cacggttagt ggtattgacg ctcaccagtg tggacaaacc aatgcgcagc gggtattgct
     5641 ccagctgttt ctggtccagt tcgatacgca caggtagacg ctgaacgact ttgatccagt
     5701 taccggtcgc attttgcgcc ggaagcagtg agaatgcgct acctgtgccc atatccagac
     5761 caaccacttt accggtgtat ttcacatcat cgccgtaaat atccgtggtg atagtgaccg
     5821 gctgaccgat acgcatattg gcaatctgcg tctctttaaa gttggcatcc acccacatat
     5881 tggtggctgg aacgaccgcc atcagcggcg tcgttgggct aatttgcgcc ccaggctgta
     5941 ccgcacggcg ggagacataa ccggtcatcg gactgacaat acgggtacgc tccagcgcca
     6001 gccaggcgtt acgtacttcg gtggcagcct gttgcacggc aggctgatct tccagtttag
     6061 tccccagaat catcgcctga ttggcattgt attgttgaat agcgacgtcc agttgcgcct
     6121 gggcactggt gacggcgtcg cgggcgtgtt gcagctcttc gcgaccaatc aggttggcat
     6181 tgcccagcgg cacacggcgg ttgtagtcgc tttgtgcttt tgcgagggcg attttctgca
     6241 cctcaatatt cgcctgcaac tgtttgctgt taatcatcag ctggtgggtt tggcgaacgc
     6301 tggaagccag tgcagttttg gctttttcaa acgcctggcg agcatctgtc gggtcgagag
     6361 tgaccagcac gtcgccttct tttacaaaat cggtgttatc ggcccagact ttcgtcacgc
     6421 tgccagacac ctgagacata atttgaatct gattccctgc cacgtatgcg tcatcggttt
     6481 cttcgaaatg acgcagtacc aaaaaccaat aaatccctat cgctacggca ataattataa
     6541 agagcaaggt gagaaggagg agcagacgct tacgtttgcc gctcttcttt accggttgct
     6601 gcggggtttg agtctccgca tttgcgctca tattgttctc cacgatcttc ttaactcatc
     6661 ggctgagccg acctgcttgc caaaaaggcc agcatgttgc gatgctggcc ggtcattttt
     6721 tcttttataa atctggattt ttgagcgaga tgacgcgtta gctcatcgct tcgagaacca
     6781 caccgtcttg ttccatctgg tcgagacggg agagcaattt gcgggtgatt tgctcgagct
     6841 gatctttttc tgttgtgctg agcgcggacc agagttgatg caggcagtta tgctgcggtg
     6901 gtaaaacctc gcgcaaaaac tcgtgacctt tttccgttaa ttgcagatgc aggcagcggc
     6961 gatcgttatc gctttcacga cgttcgatcc aaccgcgttt ttccagttca tcggcaatac
     7021 gcgtcgcgtt ggtacgggat gatccaagag cacaacttaa ttcagaaggc tgaatactgt
     7081 ggttttcctg agactccagc gtaatcaacg ccataaacaa cgtctcgtta atcccctgag
     7141 ccttcagcat tttattgcgg ttctccagca gcttgctttg catgtgcatg caaagacgag
     7201 tcagaaggat ctcctgataa ggaaaatctt cgtggcggct ggcgcgaaat tttagcattt
     7261 gttcaatggg cgtaaacgaa ctatccattt gggtatgacc tcattaattt cagccgatat
     7321 agtaacgaca gtgacaaata aagtaaatgt attgttttag aaaaatgatt cttgtgggtt
     7381 atataatcgc catcactttc caggcgagcc cataggccag cgcactaagc agtgttggga
     7441 tgataatgct gcgtgtttta tagaaactgg cacccagtac cgcgaagccg accagcgtgg
     7501 gcacgaaacg gcgtgtatcg tgcatcactt ctggtgcggt agagacaacc agcagagcgc
     7561 atatcgaggc gatgccaatg gtgtcgagca aaatacctac cgcgccacgt ttggttgggc
     7621 gggcattacc cacacgcagg cgcagcggca aatagcggaa gcaataattc gccgcgccaa
     7681 ccagtaaccc aggcagcaga acctcatagc tcatcgggcg ctccttgcca gaatgcttgg
     7741 attaacgcag tgaggcagcc acagacaatg cctgccagaa tggcgacggg aatagaaaat
     7801 agcgttacgc ctgcaagggc accaactaac gctgcggtaa cgcaaagaga ttgtttgcgc
     7861 tggaaagagg cgagcaggaa actcataaag agtgccggaa gcataaaacc taatgctgct
     7921 tcaacggcgg gataaccttg cagcaagccg ctgccagaga atgcccctat taccgtacca
     7981 aatacccacg atgaccatga actgaaggca atgccgatca tccagttctc gctccagcgg
     8041 cgattattgc gtaccagttt tgctgttgcg gcggcaaaaa cctcatccgt caggccaaac
     8101 gcccacaggg cggttttcga tttttgcaga cgctgaataa tacggctacg cagtgacggg
     8161 ccatacaaca catggcgaac atccattgcc atgacggtca gtgcagcaac ccacaaacta
     8221 ctcccggctg ccagcatcgc ggtaatgacg aactggctcg cgcctgcata aatgatgcag
     8281 gagaaaaaaa cgctttcgag aggagagaat cccagacggg tcgcattcag accgaacgca
     8341 aaggccaccg gaatataact aataacaatc ggtaaactgt ctttgcatcc ttccatgaag
     8401 gtcgccgaac caggagcagg ctgtggagta gggctttcca tagagtgtac gcttaacgat
     8461 tgttaatatt ataaatagac tgaatgaata tcttaacctt atcagactga tgggcttctt
     8521 aacaccctta taagtgtaaa gccacgaaaa cggttgctga ttgcttaatt tgcggcttcc
     8581 tgacgatgaa aacctcgcca ccagaccagt aagttaacta gggcaatcgt cgcgccagcc
     8641 agacaaacgc cagcccaacc gccatgttgc caggctgagg ctgaaattag cgaaccggcg
     8701 gcaccgccaa taaagtagct ggtcatgtaa cctgcggtca ggcgattacg cgcatcaggg
     8761 tgtattcgat aaattaccgt ctggttagtg atatgcacgc cctgtacggt gagatccagc
     8821 accaggatgc cgataatcag cgccagtacg gaagtgtgac caaaccagat cgccagccat
     8881 gaaagtaata gcagcagcag accgaaagtt gtggtgtggt gcgatttacc cttatcggca
     8941 aaaccgcccg ccggacgagc gcccaacgct ccggcagctc ccgcaagtcc aaacagacca
     9001 atgacaccat cgctgtagtt aaaaggtgga gcggcaagca aaaaggccat tgaggtccag
     9061 agaatgctga agttggcaaa ggtcaggcag cccagcaatg cgcgggtgcg gaggatctta
     9121 tcgctgataa acatgctgaa aacggaacct aacaactgtg ggtagttgag gtgggtttct
     9181 gatttcattt gtggcagacc acgccataat gctagcgcca tcagtgccat taacaccgag
     9241 gcaacccaaa agacggtgcg ccagccgccg aggtttgcca gtaacccggc aacggttcgt
     9301 gccagcaaga tccccaacag cagaccgctc ataatggtgc caaccacttt gccgcgttta
     9361 tccggtgaag ccagcgtcgc tgccagcgga accagaattt gtgccacgac tgagaataaa
     9421 ccggttaatg ccgtaccgag gatcatcatc gccagcgact gactgctggc agtgattaac
     9481 atgccgcctg ccgccagtaa ggtcatcgag acaatcaggc ggcggcgttc aaacatatca
     9541 ccgaggggaa caagaaacag cagacctgct gcatagccca actgcgcggc ggtaacaata
     9601 aagcctgccg aactggcgga aagggaaaag ttacgcgcga tggtgtcgag caatggctgg
     9661 gcgtaatagt tactggctac cgcctgaccg gtggcgatag acatcagcac gatcagcgcc
     9721 gggctaagct catgattagg tttagtcatt atttttgttc atcaggtgat gattaagctg
     9781 gaaatcaata atagcggatg ttgatgaatg ggggcgaaag atagcttgtt gaaatgtgcg
     9841 atgtggtttg taggcatgat aagacgcgcc agcgtcgcat caggcattgt gcacagaacg
     9901 gcggatgcgg cgaaaacgcc ttatccgcca tacggaatga gtgtattttg taggcatgat
     9961 aagacgcgcc agcgtcgcat caggcattgt gcacagaatg gcggatgagg caaaaacgac
    10021 ttatccgccc gaataaaaat tacttctgcg ctgccagcgc ctcattcacc cagccatcga
    10081 actgctgctg gtgggctttg atccaaccat caacatggcc ctgaatatcg ccttctgagg
    10141 ctttgccgtc atgcataatg gcgttctggg cgttaatatc tgccactggc aactgcataa
    10201 tggcaaaaag tttcgctgct gccgggtttt tctcggccca ggctttgttg gcaacgatat
    10261 gcatggtgct gaccgggaag ccataattcg caccattcgg cagtttggta tcggcgtttt
    10321 tatcgcccgg cagtgcggag aacggcacct gcaaccagac cacatctttc cctggcttca
    10381 gctcattact cacccagtac ggcgtccagg tgtaataaaa caccggtttg ccctctttgt
    10441 agcgactgat ggtgtcggcc atcatcgctg cgtagttccc ctgattatgc gtcacggtgt
    10501 tggtcagttc atacgcggca agctggtggt tgatcgcacc ttcgcagccc cagcctgggt
    10561 tacagccggt taaatccgct ttaccgtcgc cgttggtatc gaacagtttg gcgatcttcg
    10621 gatctttcag ttgtgcgatg ttggtgattt tgtactggtc ggcggttttc ttatcgatca
    10681 ggtaaccctg tgccgcgccg ttaacgaata tcccttcaca ataaaatttc ttatcgccac
    10741 cggcagcttc gtacatgttg tcatgcagcg gcgtccagtt cacggcggtg aaggttgcat
    10801 cgccggaagc aagtgaggtg tagccaacgt tgtaatccac ttcgctgggt ttattgacgg
    10861 tataacctaa tttctccagt gcacgactga ccagcagcgt ctggaaggtt tcttcagtga
    10921 tggtgctctg aactggatta acagtaatgc ctttgcccgg cagatcggct gcaaaagttt
    10981 gtgtagagat aagcgtggca aacgctgtcg caaaaagtac gctatgtcgc attgttattc
    11041 ctttttttgg cagggtgata atgcccggcg ttaccgggca agtgcagagt tacttaatga
    11101 atgggcgggt cagcagaccg acagggccag tggtgtacca gcgacggttg ccgcgactgc
    11161 gtgagtcgcg cccaacggcc tgcgtcaggc ggtcgaggat aatggcgagg atcacaatcc
    11221 cgacgccgcc aacggtggca aggcccatat ccagacgacc gataccgcga agtaccatct
    11281 gacccagccc gccgacggca atcatcgagg cgatgaccac catagaaaga gccagcatca
    11341 gcgtctggtt aacgcccgcc ataatggtcg gcatcgccag cggtaactga actttgaaca
    11401 gcatctggcg cgggctggca ccgaatgagc gcgaggcttc aatcagatcc gccggaacct
    11461 ggttaatccc cagaatcgtc agacggataa tcggcggcag agcaaagatg atcgtcacca
    11521 ctacgcccgg cacgttaccg ataccaaaca gcatgacgat tggtaccaga taaacaaacg
    11581 ctggcgtggt ctgcatggca tcaagcagtg gacgaataat tttcgccgct cgcggacttc
    11641 tcgccagcca tatccccaac ggcaaaccga tgacgataca gaacagcagg gcggttaaca
    11701 ccagcgccag agtcaccatt gcctgcgacc aggcaccgat tgcgccgatg gcaatcagcg
    11761 aaaccagcgt cgccacgccc attccgaccc cggaaatctg ccaggcgatg agagcgaaaa
    11821 cgataatcgc cactggcgct ggcataccca gcagcaattg ctggaaactg ttgaggatat
    11881 aatcaaccgg aacgcgcacg ccctggaaga cgggacggaa atgggtaaca acccagtcga
    11941 tcccttcagt gacccaactg tcgagcggga tcagcgtttt atggaacgga tcgagaatat
    12001 taaaatgctc gacgtttggc gcaggcgtac tggtcagcca gtcagcacca ccgccgtcag
    12061 tcggtgcagt cgtcggtgta ccccaggcgt ctgcggattg cgcggcactg tccgccgctg
    12121 gcgtggtatc ccacggatta ttttgatcag ccattattta ccccctcacg atctaaagcg
    12181 cgcagcagca ttcctttcga aatgatgccg acatactgtt ggtcctcgtc gaccacgggc
    12241 accgcacagg gtgcctgtcc gacatgagag agcaactcgc taagaggcgt ttgtgcatcg
    12301 actgctaacg gcgcatcaat cagcgccgca tcaagacctt gctgctgcgt taacgcggtt
    12361 ttaagcgaat cgatggagac tgcgccgaca aacttattac cgcgttcgat aacgtagcca
    12421 tattcgcgat cttcatcctg caataatttc agtgccgaac gtgggccgaa gccaggggtt
    12481 ttacgaatta agccattcgg tgtccggcgg gcaatatctt tcgcactgaa tacctgacta
    12541 atatcaacgc cacggaagaa ggtacggaca taatcattcg ccggattatt gagaatttca
    12601 tccggtgtgc cgacctgtac cacttcacca ttttgcataa tggcaattcg gtcgccaata
    12661 cgcatggctt catcgagatc gtgggaaata aagacaatgg tgcgctgatg tttcgcctgt
    12721 aattttacca gctcatcctg catctcggtg cgaattaatg gatcgagcgc cgagaaggct
    12781 tcgtccatta ataaaatatc cggattaatc gctaacgcgc gggctaatcc cacacgttga
    12841 cgcatcccgc cagagagttc atccgggtag ctgtgggcat aattttccag cccgacctga
    12901 cgcagtgcat caagggcttt ttcccggcgt tcttcggcat taattccggc caattccata
    12961 ccgaacgcag tattgtccag cacggtcata tgcggcatta aggcaaagga ctggaagacc
    13021 atcgcaatct tttttctgcg cacctcacgg agtttggcat cagatatttt ggcaatatcc
    13081 acaccatcaa tcagcacttg cccgcgggtg ggttcaatca ggcgattgag aaggcgtacc
    13141 attgtggatt tacccgagcc ggataatccc atgatgacaa atatctcgcc ttcttcaatg
    13201 gccagactgg cgtcttttac gccaagcgat agcccagttt tttccagaat ttgttctttt
    13261 gaaagtcctt gttcgatata tttgaatgct cgctgtggat gctcaccaaa tattttataa
    13321 aggtttttaa tttctaattt aattgccatg caatagaaag attcctgtta tttgtttatg
    13381 tcgatatatt acccaatgga aatagttact tttttctacc ctaacatact gagaatctga
    13441 ggcaacccct gatggcaaat gtggcatgaa tattgatgcg agcaaatcgc cggaatttcc
    13501 cgtgatataa gggctgagag caaatcgaga aaagttgata ttttttgtca aagatagcga
    13561 aaaaacgcct gaatattgtg gtttcaggcg aataatgcaa cagagtatca gtgtagatca
    13621 ccacaaaata tttgcgtgat aaaaagctat ttggcgggaa ttatttccct gtcgcgggta
    13681 gtgatatttt tgaaaataac accctcagaa attccagtct tcatcttctg tttcaaccgc
    13741 tttccccatc acataagagg agcctgaacc ggaaaaaaaa tcgtgatttt catcggcatt
    13801 cggcgaaagc gcggcgagga ttgccggatt cacttccgcc atttctgcgg gaaataaagg
    13861 ttcgtagccc agattcatca aagccttatt ggcgttgtaa cagagaaacg ctttcacatc
    13921 gtcagcccac ggggtttcgg cgtacagctc atcggtgtat tgcagctcgt tgtcgtagag
    13981 ttccagcaac aaatcgaagg cgaaactctt caactcttca cgttgtccca gagatatctt
    14041 ttccatgttt ttctgatatt tatagcctat gtagtaaccg tggactgctt catcacggat
    14101 aatcagacgg atcaggtctg cggtattggt cagctttccg cggctggaaa aatacatcgg
    14161 cagccagaaa ccggaataga acaaaaaaga ttcaagaaac acactggcga ttttcttttt
    14221 cagcggatca tcaccgcgat aatgttgctg aataatctga gcttttcgct gcaacggtgc
    14281 gttttcttca ctccaggcgt aggcggcatc gacatctttg gtctggcaca gcgtcgagaa
    14341 aatcgaactg taagagcggg catgaaccgc ttccataaag ctgatattcg ataataccgc
    14401 ttcttcatga ggcgtgagtg catcgggcat cagcgaaggc gcgccgataa cattttgcag
    14461 cgtgtcgagc agcgtcaggc cagtaaaaac gcgcatcgtc agttgttgtt ctacgacagt
    14521 taatgtctgc caggcaggaa tatcgttcga cagcggcacc ttttctggta gccagaaatt
    14581 gctggtcagg cgattccaca cctccagatc tttatcgtca gatatcttgt tccagttgat
    14641 ggcgctgata cgtgagagtt tcatagatat tccttaaagt gcacaggaga cgcagccttc
    14701 aatttcagtg ccttccagcg ccatctgacg caggcgaatg taatagagcg ttttgatacc
    14761 tttgcgccag gcgtaaatct gcgctttgtt gatatcgcga gtggtggcgg tatcggggaa
    14821 aaacagcgtc agcgacaaac cctgatcgac atggcgagtc gcttccgcgt aggtgtcgat
    14881 gatcttttct gcgccgattt cgtaagcatc ctgatacagc gccagatttt cgttagtcat
    14941 aaacggggca gggtagtaaa cgcgtcctgt tttgccctct ttgcgtatct ctactttcgc
    15001 cacaatcgga tgaatactcg acgtagcatg gttgatataa gagatagaac cggttggcgg
    15061 caccgcctga agattctggt tgtaaatgcc gtagcgcatc acgtcgtcgc gcagttgcgc
    15121 ccacatctca cgggtaggta acgtaatacc gctgcgggca aacagttcgc caactttcgc
    15181 cgttttcggc tgccagttac cttgcagata ttggctaaaa tattcaccac tggcataacg
    15241 tgactgtttg aacccggcga aggtttcacc gcgttcgcgc gccagcaaca tcgaggtacg
    15301 cagtgcgtgc caggtgatgg catagaaata gagattggtg aaatccagtg cttccggcga
    15361 accataagcg atgccttctc gcgccagata gccgtgtaaa ttcatctgcc ccagtccgat
    15421 ggcgtgtgag gcggcatttc cggcttcgat ggacggcacg ctgcggatat gactcatatc
    15481 tgacaccgcc gttaaaccgc gcacggcagt ctctaccgtg cgggcaaaat cgggggaatc
    15541 catggtgtgc gcaatattca acgaacctaa attgcaggag atatcatggc ctgtgcgggc
    15601 atagtcgaga ttctcgtcat actctgaggc acagttgacc tgcaaaatct ctgagcagag
    15661 attactcata tttatgcgcc cggcgacagg gttagcacgg tttaccgtgt cttcatacat
    15721 gatgtaggga tagccggact caaactggat ttctgccagc cgctggaaga aatcacgggc
    15781 gttgaggtat tttttgcgga tgcgttcatc ggcaaccagt tcgtcatagt gttcgctgat
    15841 ggcgatatcg gcaaacggct tgccataaac tcgctcaacg tcatagggcg aaaacagcgc
    15901 catctgcgca ttctcttttg ccagatggaa agtgatatcc gggatcacca cgcccagcga
    15961 cagtgtttta atgcggattt tttcgtcggc attttcccgt tttgtatcga gaaaacgcag
    16021 aatatcggga tgatgagcat gtaaatagac tgcacctgcc ccctgacgcg cgccgagttg
    16081 gttggcatag gaaaatgcgt cttccagcat tttcatcacc ggaattacgc cagaagattg
    16141 attttcaata cgtttaattg gcgcgcccgc ttctcgcaga ttcgacagca aaaatgccac
    16201 gccgccgccg cgtttcgaca gttgcagtgc ggaatttacc gcccggccaa tcgactccat
    16261 attgtcttca atacgcagca gaaaacagga aaccagttcg ccgcgctgct gcttaccgca
    16321 gttgaggaat gtcggcgtgg ctggctgaaa gcgtcctgac agcatttcat tggtcagttg
    16381 caacgccagc gtcgcatcgc cttgtgccag cgtcagcgcc accatcgtga ctcgatcggc
    16441 aaaatcttcc agataacgtt taccgtcgaa tgtcttcaac gtatagctgg tgtaaaactt
    16501 ccatgccccg aggaatgtct ggaaacgaaa accgctggtg tgtgcgtggg caaacagcgt
    16561 aatgacaaag tcgcgagagt agcgattaag aacgctttca tcatagtaac cttcgttgac
    16621 cagccagttc aggcgctgct gctggctact gaaggtcaca ctgttcggac gcacatgcgt
    16681 cgccataaag gcgtcaacgg cctggcggtc tttatcgaac tgaatgcgac ctgcgctatc
    16741 gtagaggtta agcatcgcat tcagcgcgtg gtaatccatc gtttcctgcg tcaggcattc
    16801 tgcggttgtc gttgccaaaa ttcggttact cctttacgaa cgttttcgat atcgctttgc
    16861 gtacccatga gttcaaaacg gtacagccac ggcacgccgc atttccgggc aatcacatct
    16921 ccggcgcggc catacgcctc accaaagttg cgattaccag aagcaataac gccgcgaagc
    16981 aacgcccggt tgtgctcgtc gtttaaaaag cgaattacct atcgtggcac cgcgccagcc
    17041 gtaccgccgc cgccgtaaga gggcacgatc aggatgtaag gctcgtctac ctgaatccgt
    17101 tcccgctcat tgagcgggat gcgcaccgcg ggcagaccta aacgttcgat aaaacgctgc
    17161 gtgttttcgg agctgctgga gaagtagacg agctggctca tgcactggcc gcgtgtggcg
    17221 ctggatgcag acggttaatc atgtccggac ggaaaccaga ccagctaaga tcgccagcaa
    17281 tcactaccgg caactgacga aagccctgag cacgcaacgc ttccgctgct tcaggaacgc
    17341 gatcgacatt aatcatttca aaatcaaagc cccggttttc catcgcccgt ttggtggcgt
    17401 ggcactgaac gcaatcgtta cgagtgtaaa tagttatgcg catgattcgt atttccgttt
    17461 aaaatgaaga tacggcgcga tgatacgcgt cgggttgtct ctctgttgat acagagatac
    17521 tagatgtagt tgaaaaaaga ttcaaccaca caatatatag caaatagtga tcgaaattac
    17581 cctagatatg agcgtgacgg gggaggggga tttttgtgat tcaccaggca aaaagaaacc
    17641 ccgaagacag gcttcggggt taaagacgcg tatttattat catttttgca ctacgatttg
    17701 cgcatgctta acagtgcgcc gataaaaata cctaccgcag ctgctgtacc cacgctacac
    17761 cagggtcttt cacgaacaaa agaatccgcg cagccaacgg catcgcgtgc ggcttgctgg
    17821 acgcgagtac gaccatgcat tcgcgccctg gtttctttca gcaatgcctg agctttactg
    17881 cgtgcggctt cagcttcccc tttggcgtcg ctgccccagg acttcaatac agattcgagg
    17941 ctgtcagcta attgattgac atcattctgg atatcctgca cgccatcatc aacgtcgttg
    18001 cggttcggtc ggttaaacat atgatccccc attagaattc gatgtaattt tcagtgtaga
    18061 ccataatttt cttaactgag gttcatcgcg gctttatgga cttttctgaa agcgttgtga
    18121 atgcgcaatc aggtaaggta ggcccgcggt tgataataac gaggatcaaa tatgtattta
    18181 cgaccagacg aggtggcgcg cgtacttgaa aaagtcggtt ttactgtcga tgtggtaacc
    18241 cagaaagctt atggttaccg ccgtggggaa aattatgtct atgttaatcg cgaagcgcga
    18301 atgggacgta ccgcactggt aattcatcca acattaaaag aacgtagttc gacactggct
    18361 gaacccgctt ccgatattaa aacttgcgat cattatcaac aatttccgct ctatttagca
    18421 ggcgagcgac acgagcatta cggtatcccg catggcttca gttcgcgtgt tgcgcttgaa
    18481 cgttatttga atggtttatt tggcgaagcc agttaaagac gcgactggcg atgccagtcg
    18541 cgaaaagaag agtcaggctt ttacctgctg gtaatggctg actttaaaca gtcggcggca
    18601 ataatcgagg aagtagccat aaaccgcccc catcaacatc gaaacaacga tgtttgaact
    18661 gaccgccgcc ataatctgat gccagtctgc gcccaccact aacaagatcg ccacatacac
    18721 cggtgactgg aacgtcacat aagccaggat atcagccaga ttttttatcc agcccgacgg
    18781 gctaactttg cgtgccgcgc gcataaatag atcgcggtac ataccgtatg gccatgcaat
    18841 taagatgttc accggaatcg ctaccaatct ggaataaaaa gactgttcga agctcattcc
    18901 ggagaggaaa acttcaatac acatgttcac gacagaacag taaacaacca tcgcgaacgt
    18961 atctgcaact gcatgacgca agcgtgactg cggtgagaac atgctaatgc tccttaataa
    19021 aagcgtaatg ggatgttaat ggagatgaaa gcagtggatc tcattgccat ctgcattttc
    19081 gcatagcgta tatcgctggc tatgactatt caattagtga aaaattttta tttagtcagt
    19141 ttttgtagat aaaatattcc gtaacggctc gttttttgta cattgtggat ttttacatgc
    19201 tgatatgatt tattttcaat taaatcaatt agatatgacc ttgcctttaa tttcactaat
    19261 gaaagtttgg gggtgaggta acgctataag cgtagagaat tcggtgtatg tcgtgaccgt
    19321 gtcatttaca gcatttttga tgccagaaaa tgtacattaa cagcagaaag caccagt
//
LOCUS       DAZSBF010000066        19079 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZSBF010000066 DAZSBF010000000
VERSION     DAZSBF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.067"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..2389
                     /gene="gcd"
                     /locus_tag="ACHXWD_001830"
     CDS             <1..2389
                     /gene="gcd"
                     /locus_tag="ACHXWD_001830"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR7620762.1"
                     /translation="AINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYPI
                     AGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGIW
                     LILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAIS
                     PVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEVT
                     PIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAET
                     ASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYEP
                     TSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSDE
                     HTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATTG
                     KLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELVV
                     PAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQMR
                     YEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGNP
                     MEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEVV
                     WKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSNG
                     EKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2436..3986)
                     /gene="cueO"
                     /locus_tag="ACHXWD_001831"
     CDS             complement(2436..3986)
                     /gene="cueO"
                     /locus_tag="ACHXWD_001831"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR7620763.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            4152..4499
                     /locus_tag="ACHXWD_001832"
     CDS             4152..4499
                     /locus_tag="ACHXWD_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR7620764.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4605..5471
                     /gene="speE"
                     /locus_tag="ACHXWD_001833"
     CDS             4605..5471
                     /gene="speE"
                     /locus_tag="ACHXWD_001833"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR7620765.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            5487..6281
                     /gene="speD"
                     /locus_tag="ACHXWD_001834"
     CDS             5487..6281
                     /gene="speD"
                     /locus_tag="ACHXWD_001834"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR7620766.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(6319..6681)
                     /gene="yacL"
                     /locus_tag="ACHXWD_001835"
     CDS             complement(6319..6681)
                     /gene="yacL"
                     /locus_tag="ACHXWD_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR7620767.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(6856..9453)
                     /gene="acnB"
                     /locus_tag="ACHXWD_001836"
     CDS             complement(6856..9453)
                     /gene="acnB"
                     /locus_tag="ACHXWD_001836"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR7620768.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKIEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            9808..11565
                     /locus_tag="ACHXWD_001837"
     CDS             9808..11565
                     /locus_tag="ACHXWD_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR7620769.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(11636..13060)
                     /gene="lpdA"
                     /locus_tag="ACHXWD_001838"
     CDS             complement(11636..13060)
                     /gene="lpdA"
                     /locus_tag="ACHXWD_001838"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR7620770.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13385..15277)
                     /gene="aceF"
                     /locus_tag="ACHXWD_001839"
     CDS             complement(13385..15277)
                     /gene="aceF"
                     /locus_tag="ACHXWD_001839"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR7620771.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15292..17955)
                     /gene="aceE"
                     /locus_tag="ACHXWD_001840"
     CDS             complement(15292..17955)
                     /gene="aceE"
                     /locus_tag="ACHXWD_001840"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR7620772.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18116..18880)
                     /gene="pdhR"
                     /locus_tag="ACHXWD_001841"
     CDS             complement(18116..18880)
                     /gene="pdhR"
                     /locus_tag="ACHXWD_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR7620773.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4402 a         5288 c         4790 g         4599 t
ORIGIN      
        1 ggcaattaac aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc
       61 gctttgcggg ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg
      121 gtactaccct atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa
      181 acgcgccgcg ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg
      241 ggaagttggt ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg
      301 catctggctg atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt
      361 tgccgcactg gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa
      421 cgatccgcag gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc
      481 acccgttgcc gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc
      541 acctctgaaa caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg
      601 tactggcgat gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat
      661 taaagtgggc gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc
      721 cgccagcggc aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca
      781 gcacgtaacc tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga
      841 agtgatggcg gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc
      901 gattaacgct gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct
      961 gcaaagcaat atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat
     1021 caccgataaa accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac
     1081 gtctggcgtg atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc
     1141 cggcgcgaaa gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc
     1201 aaactcctgg gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg
     1261 cgtgaccacg ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag
     1321 ctcgattctg gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca
     1381 ccacgacctg tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa
     1441 tggtcagaaa gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga
     1501 tcgtcgcaat ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc
     1561 gaaaggcgat tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa
     1621 agatttgagc ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt
     1681 gatgttccac cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct
     1741 ggtcttcccg ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg
     1801 tgaagtggca attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg
     1861 tccgggtaac ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat
     1921 tcagccacag tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg
     1981 tctgccgtgt aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga
     2041 agtggtgtgg aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt
     2101 accggtgccg ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa
     2161 cgtgctgttt atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg
     2221 tgaaaaactg tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga
     2281 agtgaatggc aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa
     2341 gatgggcgac tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc
     2401 ggagagatcc gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc
     2461 cccgtatctt catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg
     2521 ttgtgattaa acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc
     2581 cagcccgcgc gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg
     2641 ccgtgaatat ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg
     2701 tactgccctt tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt
     2761 ttattggcat ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc
     2821 cccatatggc ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc
     2881 tccattagca tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg
     2941 cgtaccgtca gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa
     3001 gcaccggagg cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac
     3061 ggcgcaatcg ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg
     3121 tcgttaacct ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc
     3181 ttcaccggtt caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg
     3241 ctggtggcga aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag
     3301 ccacgcgggg cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac
     3361 cagcccacgg cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat
     3421 tttttatcct gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat
     3481 ttcaggatct cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc
     3541 ccggtcttgc catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc
     3601 aacgtcaccg tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca
     3661 cccggtactt ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata
     3721 tcaaccgtta ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta
     3781 tagccccagg tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac
     3841 tgaatgcgat tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc
     3901 gccgcaaaga ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa
     3961 tattttaaga aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa
     4021 atcaaacgtt gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa
     4081 tcgacaataa tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc
     4141 tggtagaagc aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct
     4201 gcgcaaacag ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag
     4261 tttttgtctt tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc
     4321 gcgcactggt ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg
     4381 acatgaaagc cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg
     4441 ctaaaaagtg caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat
     4501 ccctgatacc tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg
     4561 ttgcgccctt tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac
     4621 agtggcatga aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt
     4681 atcatgaaaa gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg
     4741 taatggcgct ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga
     4801 tgatgaccca tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg
     4861 gcggcgacgg tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga
     4921 tggtggaaat cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg
     4981 ccggtagcta cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta
     5041 atcaaaccag ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg
     5101 gcgaaagtct gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg
     5161 gtatcttcgt cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct
     5221 atcgcaaact cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct
     5281 attacggcgg tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct
     5341 caaccgaaat tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc
     5401 cggcaatcca tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac
     5461 agccgtccta aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc
     5521 tgaccaaaag tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc
     5581 gcgacggtta tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc
     5641 tgtcagaaac ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg
     5701 aaccacaggg tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca
     5761 tcgacaaaac agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa
     5821 gtcatatttg cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc
     5881 gcgccgatat tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc
     5941 tgatccacca gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc
     6001 gcgacattaa cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta
     6061 tgtctgacga tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata
     6121 tcttccatac caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca
     6181 aaccggaaga tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa
     6241 tgcgcgagat ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc
     6301 aggctccgcc agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg
     6361 cagaaaatcc tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc
     6421 ttccatttca tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc
     6481 catccacagg gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc
     6541 gtgcgctgct tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa
     6601 ccagtgcccg accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc
     6661 gcgcagaaat tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca
     6721 ttgcgctatt gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt
     6781 ttagaaataa aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc
     6841 gcgtgcgctt acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta
     6901 ctgagaaagc tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg
     6961 cgcaacgtag gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc
     7021 cgccagttct gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt
     7081 acgggtagag gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca
     7141 cagagaacag cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc
     7201 ttcttcagtc aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg
     7261 caactggcct ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt
     7321 catgcaagaa ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg
     7381 tgcgtcgtcc gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat
     7441 gtcgatcact gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag
     7501 ccatttttcc atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc
     7561 gatcatccac ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt
     7621 cagcttgata gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc
     7681 ctgctcaatt ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt
     7741 tttcttgcct ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc
     7801 gtgtaccaga tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac
     7861 ggattccggc atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga
     7921 acccgccggg aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac
     7981 ggtatccggc agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag
     8041 cgacacacca ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac
     8101 tggcttcgga tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc
     8161 caggcacgcc aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc
     8221 tacagaagtc attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc
     8281 acgacctacc attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc
     8341 ctgacggaac acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc
     8401 acgcccgata atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt
     8461 tttcagttcg aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta
     8521 cgggtaaacg tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag
     8581 tgcacccgcg tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa
     8641 accaccgccg cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga
     8701 gttcgtggcg gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg
     8761 gaaacctttc tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc
     8821 tggctcaata ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg
     8881 gcgcgaccac gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt
     8941 gaagacggta acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc
     9001 cgcccaggac tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac
     9061 gtcatagaag ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc
     9121 cagtttggca tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt
     9181 acccagcagt tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc
     9241 gatagcagcc aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg
     9301 gttggttaat agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc
     9361 tacaagtgcg gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg
     9421 ctcagctacg tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat
     9481 agtgcggcag gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc
     9541 agtaccgcaa cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag
     9601 caacataaaa aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa
     9661 acttcgttaa ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa
     9721 tttaaactac cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac
     9781 cattgcgatt tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg
     9841 ctggcactaa tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc
     9901 cgcacagtgg aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc
     9961 gcagttacgc ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc
    10021 gcattcagca ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct
    10081 ctgctttcac aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa
    10141 tggtcgcacg ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag
    10201 ccgtgggacg ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc
    10261 gaaaacccgc aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg
    10321 atggactcgg tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca
    10381 accgaacaga aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg
    10441 gcacccgtca taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca
    10501 acaaccgtca tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac
    10561 ccaaccgtgg tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca
    10621 ccagccggag aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt
    10681 gctaccacgt acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat
    10741 catcatgacg atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg
    10801 caacacaacg gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag
    10861 catcttactg ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc
    10921 tatcatgatc aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt
    10981 gccacgcagc tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt
    11041 cagcaacgaa cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca
    11101 cagcggttta atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt
    11161 cgccaaccac tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct
    11221 cctgagcagc gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga
    11281 agagaggcag cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag
    11341 gaaaaagtac agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata
    11401 caatcggcat cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca
    11461 caacgtctaa acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta
    11521 cgcgaacgtc tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa
    11581 aaagcggcgt ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact
    11641 tcttcttcgc tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca
    11701 ggcccacaga ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat
    11761 cacaacccat ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga
    11821 caatcgcacc accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt
    11881 ctgcgcagtc ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat
    11941 agctgatgcc tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt
    12001 cggtataggc gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa
    12061 cttcagcggc aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga
    12121 tatcgccgat agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga
    12181 aaccacggtc gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca
    12241 cacgaccaat cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc
    12301 cttccatcgt cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca
    12361 gcatcaggtt gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag
    12421 ctgccgggat aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt
    12481 atacggtgcc catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta
    12541 cttctttcag ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa
    12601 acggcagttg gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg
    12661 ttttgccgtt ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt
    12721 tgaccacttt gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga
    12781 tcactttctc tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga
    12841 taccgtgttc agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt
    12901 tagaagggat acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga
    12961 ttacggtttc cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg
    13021 ccccaagtac cacgacctga gttttgattt cagtactcat catgacctct atatatttat
    13081 ctccggcggt catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc
    13141 ccagtcactt acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca
    13201 gaaatccata gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg
    13261 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    13321 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    13381 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    13441 gggcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    13501 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga
    13561 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    13621 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    13681 ctttcttaga aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt
    13741 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    13801 atttcttcag ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct
    13861 gctcaagagc tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca
    13921 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    13981 cggttttgtc gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac
    14041 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt
    14101 ccaccttcgg ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt
    14161 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    14221 tacgaccagt gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga
    14281 tcagcggagt cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt
    14341 tcgcagccgg agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt
    14401 cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    14461 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    14521 gagccgggac ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg
    14581 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    14641 ccggaacgtt aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt
    14701 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    14761 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa
    14821 acggagccgg aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag
    14881 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    14941 tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt
    15001 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    15061 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    15121 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    15181 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    15241 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    15301 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    15361 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc
    15421 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    15481 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    15541 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
    15601 gcgcggagtt tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc
    15661 cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    15721 gatctctgcc gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt
    15781 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    15841 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    15901 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc
    15961 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    16021 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    16081 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
    16141 gaagccgaac atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta
    16201 agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag
    16261 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta
    16321 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    16381 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    16441 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    16501 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    16561 gcttggcaga taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga
    16621 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    16681 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    16741 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    16801 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    16861 gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc
    16921 ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    16981 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    17041 tttacgcagc agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc
    17101 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    17161 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
    17221 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    17281 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    17341 ctggtaaata gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc
    17401 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    17461 gttatccagc tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg
    17521 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    17581 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
    17641 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    17701 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    17761 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    17821 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    17881 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    17941 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    18001 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    18061 ttttatatac gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    18121 tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
    18181 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    18241 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    18301 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    18361 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    18421 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    18481 tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    18541 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag
    18601 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    18661 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    18721 cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga
    18781 gtttttcgcc cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca
    18841 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    18901 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    18961 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    19021 gacatatata acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaag
//
LOCUS       DAZSBF010000067        18840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZSBF010000067 DAZSBF010000000
VERSION     DAZSBF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.068"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..99)
                     /locus_tag="ACHXWD_001842"
     CDS             complement(<1..99)
                     /locus_tag="ACHXWD_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620774.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPT"
     gene            complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="ACHXWD_001843"
     CDS             complement(250..2121)
                     /gene="ppiD"
                     /locus_tag="ACHXWD_001843"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR7620775.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2313..2603)
                     /gene="hupB"
                     /locus_tag="ACHXWD_001844"
     CDS             complement(2313..2603)
                     /gene="hupB"
                     /locus_tag="ACHXWD_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR7620776.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2794..5148)
                     /gene="lon"
                     /locus_tag="ACHXWD_001845"
     CDS             complement(2794..5148)
                     /gene="lon"
                     /locus_tag="ACHXWD_001845"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR7620777.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="ACHXWD_001846"
     CDS             complement(5336..6610)
                     /gene="clpX"
                     /locus_tag="ACHXWD_001846"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR7620778.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="ACHXWD_001847"
     CDS             complement(6736..7359)
                     /gene="clpP"
                     /locus_tag="ACHXWD_001847"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR7620779.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7605..8903)
                     /gene="tig"
                     /locus_tag="ACHXWD_001848"
     CDS             complement(7605..8903)
                     /gene="tig"
                     /locus_tag="ACHXWD_001848"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR7620780.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="ACHXWD_001849"
     CDS             complement(9247..9564)
                     /gene="bolA"
                     /locus_tag="ACHXWD_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR7620781.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9869..10447
                     /locus_tag="ACHXWD_001850"
     CDS             9869..10447
                     /locus_tag="ACHXWD_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7620782.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10491..11966
                     /gene="ampG"
                     /locus_tag="ACHXWD_001851"
     CDS             10491..11966
                     /gene="ampG"
                     /locus_tag="ACHXWD_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR7620783.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12427..13374
                     /gene="cyoA"
                     /locus_tag="ACHXWD_001852"
     CDS             12427..13374
                     /gene="cyoA"
                     /locus_tag="ACHXWD_001852"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR7620784.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13396..15387
                     /gene="cyoB"
                     /locus_tag="ACHXWD_001853"
     CDS             13396..15387
                     /gene="cyoB"
                     /locus_tag="ACHXWD_001853"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR7620785.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15377..15991
                     /locus_tag="ACHXWD_001854"
     CDS             15377..15991
                     /locus_tag="ACHXWD_001854"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR7620786.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15991..16320
                     /locus_tag="ACHXWD_001855"
     CDS             15991..16320
                     /locus_tag="ACHXWD_001855"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR7620787.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16332..17222
                     /gene="cyoE"
                     /locus_tag="ACHXWD_001856"
     CDS             16332..17222
                     /gene="cyoE"
                     /locus_tag="ACHXWD_001856"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR7620788.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17407..17718
                     /locus_tag="ACHXWD_001857"
     CDS             17407..17718
                     /locus_tag="ACHXWD_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR7620789.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17702..18244
                     /locus_tag="ACHXWD_001858"
     CDS             17702..18244
                     /locus_tag="ACHXWD_001858"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7620790.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18300..>18840)
                     /locus_tag="ACHXWD_001859"
     CDS             complement(18300..>18840)
                     /locus_tag="ACHXWD_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR7620791.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4216 a         4952 c         4579 g         5093 t
ORIGIN      
        1 cgtcggtttc gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag
       61 ggacagggta atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt
      121 aacagtggaa tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg
      181 gaaaaggccg cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg
      241 gcttcaggat tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag
      301 gttactcatc agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat
      361 cgcttttttc tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac
      421 cacattacct tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg
      481 cagtgcaaac gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc
      541 gccaaatttc agaccggcag cctgcatagc ttccgcccct ttgccggctt tcaaatcaac
      601 cagcagtttc tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc
      661 cttaacttgt tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgat
      721 gcgcagcacg aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccggcgc
      781 gccgttttca cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa
      841 ctcttccggc aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc
      901 ttgctctgca ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg
      961 cagcgcatag tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg
     1021 tacttcgtct aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa
     1081 accgaccgaa gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt
     1141 cagttcgtcg gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga
     1201 gataatatca gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc
     1261 atcaagtacc gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg
     1321 ctgcggctgg gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct
     1381 aaccggttgc tgcatcgttg cggcatccag cttgatgtaa ctcacgcgga attgttccgg
     1441 cgtcatgaaa ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg
     1501 ctgcttcgcc gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc
     1561 gaccagtgcc gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacacc
     1621 gttaatcagc tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt
     1681 catccccatc tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg
     1741 gaaggctggg gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag
     1801 ctcacgagcg tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg
     1861 cagggttttc atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg
     1921 ctgcatgcga ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg
     1981 gtcattcact tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt
     2041 caatatgaac gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc
     2101 cgtgcgtaag ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca
     2161 cgcaacgtat cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc
     2221 cacaagacac agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt
     2281 gaacttcgtc acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta
     2341 cctgcacgga agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg
     2401 ttgcggccag tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct
     2461 acatcatccc cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg
     2521 ccagccgcag ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta
     2581 ttcactcttc tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg
     2641 cgcagcagtt atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg
     2701 atccgccatc taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc
     2761 cctgttttta ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc
     2821 agacggttca ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat
     2881 gtccagatcg gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg
     2941 aattagcact gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc
     3001 gatcggcagt acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac
     3061 cgggttaccg gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc
     3121 atctttcggc gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc
     3181 agggttgatc cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga
     3241 ctcctgcatc acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca
     3301 tgcggtttca atggtcagca agtcaccgcc cacttccgtc cacgccagac cggttacctg
     3361 accgacacgg ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc
     3421 atgcaggtta tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt
     3481 aaccgctttg cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg
     3541 ggtgtagtaa cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt
     3601 cagtgcatta cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc
     3661 atcttcggta taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat
     3721 gttcatggag ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag
     3781 gtagtggtcg ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc
     3841 cggatcgcct cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcgggtt
     3901 tttcacgccc actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt
     3961 acggcggtga ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata
     4021 tttacgcccg gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg
     4081 ccctaccagg cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta
     4141 ctcaaggatg cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg
     4201 cgcctgacgc aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca
     4261 gtcgatataa ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt
     4321 ctgcaactct gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt
     4381 gcgcttcagg gcttcgtttt cgtccggcgc gtcgtccatt tcaccgagtt ctttctgaat
     4441 agctttcatt tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac
     4501 gcggttgcga atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat
     4561 cagatattcc agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag
     4621 tttcagcggc atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt
     4681 cagcgacgtc agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg
     4741 gctgattgca gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag
     4801 atactccgcc ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg
     4861 ctgtaacccc tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat
     4921 agaggccacg gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc
     4981 tttctgcgcg accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat
     5041 agatttttcc cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg
     5101 cagcggcaat acggggattt caatgcgttc agaacgctca ggattcatag agctctctct
     5161 tagtttaatt tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca
     5221 tgtacgtcag tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt
     5281 aaaggggaga taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt
     5341 caccagatgc ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat
     5401 caattaccga ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca
     5461 tggtatcgag cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg
     5521 ccatcgcttt cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt
     5581 ccagattaaa cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct
     5641 gaatcagagc ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga
     5701 actcagggat aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc
     5761 cttcgcttgc tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg
     5821 tttctacacg gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca
     5881 ggatcttaga ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg
     5941 gtggaacagc agctaccgta ccttcgatca gtttcaacag tgcctgctgt acgccttcac
     6001 cggaaacgtc tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat
     6061 cgatgtagac aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct
     6121 tctgaatgat gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt
     6181 cggccatggt gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac
     6241 cggaaccggt cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg
     6301 tatcgccgtt gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg
     6361 cctgttcctg gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta
     6421 gcgcactgcg ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac
     6481 ataaatcaac acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt
     6541 catgctggct tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt
     6601 tatctgtcat gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg
     6661 ccattctcag gcgcaagccg cttacagcgt tgtgccgccc tggataagta tagcggcaca
     6721 gttgcgcctc tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac
     6781 cgcttcaggg gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga
     6841 ttgacccgta tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg
     6901 ggcatgaatt tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat
     6961 cacgcgcgaa ttcggcaggc aaaaacgttt accttttgcc cctgcggtca gcaagaaagc
     7021 gcccatcgag gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat
     7081 ggtgtcatag atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata
     7141 gatatctttt tctgggtttt ccgcttctag gaacagcatc tgcgccacaa tcaggttcgc
     7201 catgtggtct tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata
     7261 gatatcaaaa gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat
     7321 atggggtgca aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt
     7381 gaaaaaacct gctgtaccga ttgtaacctt atggacggat tatcagctag tccctgtgtt
     7441 atgggtacgg catcaccata tttcaagcat aacaatcttt tgttgttacg ctaacaccga
     7501 aagaggcttt tcgcacggtt ccatgcaaaa ttcatgacaa aaaaaagccc accacctggc
     7561 ggtgacgggc ctttgtgcga atttagcgcg ttatgctgcg taaattacgc ctgctggttc
     7621 atcagctcgt tgaaagtggt ttctttttca gtcactttcg ctttcgccag tacagcttca
     7681 acagcctgtt cttccagagc aacattgcgc atgttgtcca tcagttcttt gtttttgctg
     7741 tagaactcga taacttcttt cggatcttcg tacgcagaag ccatctcttc gatcaggcct
     7801 ttcacgcgct cttcgtcagc tttcagttcg ttggtgcgga taacttcgcc cagcagcagg
     7861 ccaacaacta cgcggcgttt agcctgttct tcgaacagtt cgcgcggcag ttccagagct
     7921 tgtttttcgt tgccaccgaa acgctgtgca gcctggcgac gcagaacgtc gatttcgctg
     7981 tcgatcagcg cagccggtac gtcgatgtcg ttagctttta ccagaccttc gatcgcctga
     8041 gacttaacgc ggttacggat ggcgctcttc agctcgcgct ccatgttttt acgcacttca
     8101 gcgcgcagac cttctacgga accatcttca acgccgaaac gtttgatgaa ttctgcagtc
     8161 agttccggca gttcacgctc ttcaactttc ttcaggttga tagcgaattt cgctgcttta
     8221 cctttcagat tttctgcgtg gtattcttcc gggaaggtca cgtcgatggt gaactcttcg
     8281 ccagctttgt ggcctttgat accgtcttca aagcccggga tcatacgacc ctggcccatc
     8341 gccagtacga aatcagacgc tttaccgcct tcgaactctt cgccgtctac agaaccggtg
     8401 aagtcgatgg ttacgcggtc ttctgcttca acggcgccgt ctttttcttt ccaggtcgcc
     8461 tgctgtttac gcagagtatc cagcatgccg tcaacgtcag cgtcggtcac ttcaacgatc
     8521 ggtttttcaa cttcgatcgc ttccagaccc tgcagttcaa cttccggata aacttcaaac
     8581 tctacagagt aggtgaagtc ttcacccagc ttgtattcgc ccggaacata agtcggtgcg
     8641 ccagccggat tgattttttc tttaatgatg gcgtcaatga agttacggct catcaggtca
     8701 cccagaacgt cctggcgtac agacgcgcca taacgctgag caacgatatt cattggcact
     8761 ttgcctttgc ggaagccgtc aatacgtact tttttcgcaa cgttgaccag ctcgctttta
     8821 acagcggtct cgatgctgtc agcagcgata gtaatcgtta cacggcggcc aaggccttga
     8881 gtggtttcaa ctgaaacttg catcttgtta cctcaaaaaa tcacagtgct cggtcaactc
     8941 ttaccccgca agcacaggtt gttggcttcg cggaaccggg atgttcttat aatcaatcac
     9001 attccctgtt accagaatca tcccgaaagc attcaaatag gacgcggcat tatagcggca
     9061 tcacttttat gagtcgagaa cggttattgc gcgctgtcgc gtattttttc acaattcccg
     9121 ggcaattttc gtctgaatgc tgggcaaaaa gcgcaaaatc cagacaaaac aaaacggccc
     9181 gcaggccgtt tttcataaaa tctttagcaa catactggaa aagcgccgac agttgcaaat
     9241 gcgtttttac gcgatgcttc ctgctccacg acagggagga gaggcaaaga cggtgtcctg
     9301 caacccttcc cactccttaa tagtgtaagt atgcagagcc agcgcatgaa cggtagtaga
     9361 gagttcctcc gctaaagtac tgtaaatcat tcgatgacga ttcagaaaac gttcacccgt
     9421 aaaacgatcg ctgaccagca caactttaaa atggctttca gagccggctg ggacattgtg
     9481 acgatagctt tcatccacta cttcgaggaa tacgggttgg aacgccgccc ttaatttttc
     9541 ttctatccgc tcacgtatca tcatgaaata tctcctccga caacgctgag atgtcatcca
     9601 tccctttaaa tactagccgc ttttaccgtt tccattgcag cttaacaaca aatatttagg
     9661 tttcgtcctg gtttttccgt caagcgtatg aagtttactg atttaaagat tgaaacacag
     9721 gcctcgcatc agcgtttctt ttaccagggg tgattatttc gccaacatga tgaatttaca
     9781 tgccttaccc acttcccctc gccgttggca atgttatgat ggcgggaatt tttaccaaac
     9841 acgcttaaga gtctctgaga gcccgaacat gttcaaaaaa atcctcttcc cgttagttgc
     9901 tctgtttatg cttgcaggat gcgcaaaacc gccaacaact attgaagttt ccccgacgat
     9961 tacactgcca cagcaggatc caagcctgat gggcgtcacc gtaagcatta atggtgccga
    10021 tcagcgtacc gatcaggcgc tggcaaaagt cacccgcgat aatcaaatcg ttaccctgac
    10081 cgcctcccga gatctgcgtt tcctgctgca agaagtgctg gaaaaacaga tgaccgcgcg
    10141 tggttacatg gttggtccga atggcccggt taatctgcaa atcatcgtta gccaactgta
    10201 tgctgacgtg tcccagggca acgtgcgcta caacatcgcg accaaagcgg atatcgccat
    10261 catcgctacc gcgcagaatg gcaacaaaat gaccaaaaac tatcgtgcca gctacaacgt
    10321 tgaaggtgcg ttccaggcct ccaacaaaaa tatcgccgat gcggttaaca gtgtgctgac
    10381 cgataccatc gctgatatgt ctcaggacac cagcatccac gaattcatca agcagaacgc
    10441 gcgttaattt ctgccctctg gcccggtgca aaccgggcct gtagacgccc atgtccagtc
    10501 aatatttacg tatttttcaa cagccgcgtt cagccatatt gctgatcctg ggttttgctt
    10561 ccgggctacc gctcgccctg acatccggta ccttacaggc ctggatgacg gtcgagaata
    10621 ttgatctcaa aaccattggt ttcttctctc tggtaggcca ggcttacgtt tttaaattcc
    10681 tctggtcacc gctgatggac cgctacacgc ccccattttt tgggcggcgg cgcggttggc
    10741 tgctcgccac gcaaatcctg ttattagtcg ccattgcggc gatgggtttt ctcgaacctg
    10801 gcacccaact ccgctggatg gcggcactgg cggtggtgat cgctttttgc tcggcctccc
    10861 aggatattgt cttcgatgcg tggaaaaccg atgtgctccc ggcagaagaa cgtggtgcgg
    10921 gcgcggcaat cagcgtgctg ggttaccgtt tagggatgct ggtttccggc ggcttagccc
    10981 tgtggctggc agataaatgg ctgggctggc agggcatgta ctggttgatg gcggcgctgt
    11041 tgatcccttg tattatcgcg acgttgcttg caccagaacc gaccgacacc attcctgtgc
    11101 caaaaacgct ggaacaagcg gttgttgcac ctctgcgaga tttctttggt cgcaataatg
    11161 cctggcttat tttgcttctt atcgtgctgt ataagctggg cgacgcattc gccatgagcc
    11221 tgacaaccac gtttttgatt cgcggcgtcg ggtttgatgc gggtgaagta ggcgtggtta
    11281 acaaaacgct tggcttactg gcgaccattg tgggcgcatt gtacggtggg attttgatgc
    11341 agcgcctgtc gctgttccgg gcgctgctga ttttcggcat tttacaaggt gcgtctaacg
    11401 ctggttactg gctgctgtcg attactgata agcatctcta cagcatgggc gcagccgtct
    11461 ttttcgaaaa cctctgtggc gggatgggca catcagcctt tgtcgcactg ttaatgacgc
    11521 tgtgtaataa gtcattttcc gctactcaat ttgccctgct ctcagcgctt tccgctgtag
    11581 gacgagttta tgtcggcccc gtggcgggtt ggtttgttga agcacacggc tggtcgacat
    11641 tctatctatt ctccgtcgcc gctgccgtac cggggcttat tttgctgctg gtttgccgcc
    11701 agacgcttga atatacacga gtaaatgaca actttatctc ccgtaccgaa tatccgacag
    11761 gttatgcctt tgccatgtgg acactggcgg cgggcgtcag cctgttggcc gtgtggttac
    11821 tgctgttgac gatggacgcg ctggatttga cgcacttctc tttcctgcct gctctgctgg
    11881 aagtcggggt tttagtcgcc ctttctggcg tcgtgcttgg tggtttgctg gattatctgg
    11941 ctctacgaaa aacgcatctg acgtaatctg taaatattat ttagctgtag taatcactcg
    12001 ccgtaattat tacggctaaa taaacatcag tatcgcttaa taatatttgt tcttttgtgc
    12061 ggcttagcgt ttggaaatta ttgacgcaat ttatgaattc tattttttct tacacgattc
    12121 agctaatgag tctttatttt ccccccaccc aattgtcacc ataatgataa ttatttgtta
    12181 aacaattgtt ttattcaaga ttggttatac caattgcccg tccaaactcc ttctgcactc
    12241 ccctttcgtt ataacgccct tttgcaacag cttcttaaaa tcaacttgat atgttttgca
    12301 acatatgtaa cctgacagcc aaatccaagt aacaggaatt taatcatgtt tacagtaatg
    12361 taacctcccc gtaaaatgcc cacacacttt aaacgccacc agatcccgtg gaattgaggt
    12421 cgttaaatga gactcaggaa atacaataaa agtttgggat ggttgtcatt atttgcaggc
    12481 actgtattgc tcagtggctg taattctgcg ctgttagatc ccaaaggaca gattggtctg
    12541 gagcaacgtt cactgatact gacggcattt ggcctgatgt tgattgtcgt tattcccgca
    12601 atcttgatgg ctgttggttt cgcctggaag tatcgtgcga gcaataaaga tgctaagtac
    12661 agcccgaact ggtcacactc caataaagtg gaagctgtgg tctggacggt acctatctta
    12721 atcatcatct tccttgcggt actgacctgg aaaaccactc acgctcttga gcctagcaag
    12781 ccgctggcac acgacgagaa gcccattacc atcgaagtgg tttccatgga ctggaaatgg
    12841 ttcttcatct acccggaaca gggcattgca accgtgaatg aaatcgcttt cccggcgaac
    12901 actccggtgt acttcaaagt gacctccaac tccgtgatga actccttctt cattccgcgt
    12961 ctgggtagcc agatttatgc catggccggt atgcagactc gcctgcatct gatcgccaac
    13021 gaacccggta cttatgacgg tatctccgcc agctacagcg ggccgggctt ctcaggcatg
    13081 aagttcaaag ctattgcaac accggatcgc gccgaattcg accaatgggt cgcaaaagcg
    13141 aaacagtcgc cgaacaccat gtctgacatg gctgcgttcg aaaaactggc cgcgcctagc
    13201 gaatacaacc aggtggaata tttctccaac gtgaaaccag acttgtttgc cgatgtgatt
    13261 aacaagttta tggctcacgg taagagcatg gacatgaccc agccagaagg tgagcacagt
    13321 gcacacgaag gtatggaagg catggacatg agccacgcgg aatccgccca ttaaaggggt
    13381 tgaggaagaa taaagatgtt cggaaaatta tcacttgatg cagtcccgtt ccatgaacct
    13441 atcgtcatgg ttacgatcgc tggcattatt ttgggaggtc tggcgctcgt tggcctgatc
    13501 acttacttcg gtaagtggac ctacctgtgg aaagagtggc tgacctccgt cgaccataaa
    13561 cgcctcggta tcatgtatat catcgtggcg attgtgatgt tgctgcgtgg ttttgctgac
    13621 gccattatga tgcgtagcca gcaggctctt gcctcggcgg gcgaagcggg cttcctgcca
    13681 cctcaccact acgatcagat cttcaccgcg cacggcgtga ttatgatctt cttcgtggcg
    13741 atgcctttcg ttatcggtct gatgaacctg gtggttccgc tgcagatcgg cgcgcgtgac
    13801 gttgcgttcc cgttcctcaa caacttaagc ttctggttta ctgttgttgg tgtgattctg
    13861 gttaacgttt ctctcggcgt gggcgaattt gcgcagaccg gctggctggc ctatccaccg
    13921 ctatcgggaa tagagtacag tccgggagtc ggtgtcgatt actggatatg gagtctccag
    13981 ctatccggta taggtacgac gcttaccggt atcaacttct tcgttaccat tctgaagatg
    14041 cgcgcaccgg gcatgaccat gttcaagatg ccagtattta cctgggcatc actgtgcgcg
    14101 aacgtactga ttattgcttc cttcccaatt ctgacggtta ccgtcgcgtt gttgaccctg
    14161 gatcgctatc tgggcaccca tttctttacc aacgatatgg gtggcaacat gatgatgtac
    14221 atcaacctga tttgggcctg gggccacccg gaagtttaca tcctgatcct gcctgttttc
    14281 ggtgtgttct ccgaaattgc ggcaaccttc tcgcgtaaac gtctgtttgg ttatacctcg
    14341 ctggtatggg caaccgtctg tatcaccgtg ctgtcgttca tcgtttggct gcaccacttc
    14401 tttacgatgg gtgcgggcgc gaacgtaaac gccttctttg gtatcaccac aatgattatc
    14461 gccatcccga ccggggtgaa gatcttcaac tggctgttca ccatgtatca gggccgcatc
    14521 gtgttccatt ctgcgatgct gtggaccatc ggttttatcg tcactttctc ggtgggcggg
    14581 atgactggcg tgctgctggc agtaccgggc gcggacttcg ttctgcataa cagcctgttc
    14641 ctgattgcgc acttccataa cgttatcatc ggcggcgtgg tcttcggctg cttcgcaggg
    14701 atgacctact ggtggcctaa agcgttcggt ttcaaactga atgaaacctg gggtaaacgc
    14761 gcgttctggt tctggatcat cggcttcttc gttgccttta tgccgctgta tgcgctgggc
    14821 ttcatgggca tgacccgtcg tttgagccag cagattgacc cgcagttcca caccatgctg
    14881 atgattgcag ctagcggtgc agtactgatt gcgctgggta ttctctgcct cgttattcag
    14941 atgtacgttt ctattcgcga ccgcgaccag aaccgtgacc tgactggcga cccgtggggt
    15001 ggccgtacgc tggagtgggc aacctcttcc ccgcctccgt tctataactt tgccgttgtg
    15061 ccgcacgttc acgagcgtga tgcattctgg gaaatgaaag agaaaggcga agcgtataaa
    15121 aagcctgacc actatgaaga aattcatatg ccgaaaaaca gcggtgcagg tatcgtcatt
    15181 gcagctttct ccaccatctt cggtttcgcc atgatctggc atatctggtg gctggcgatt
    15241 gttggcttcg caggcatgat catcacctgg atcgtgaaaa gcttcgacga ggacgtggat
    15301 tactacgtgc cggtggcaga aatcgaaaaa ctggaaaacc agcatttcga tgagattact
    15361 aaggcagggc tgaaaaatgg caactgatac tttgacgcac gcgactgccc acgcgcacga
    15421 acacgggcac cacgatgcag gcggaaccaa aatcttcgga ttttggatct acctgatgag
    15481 cgactgcatt ctgttctcaa tcttgtttgc tacctatgcc gttctggtga acggcaccgc
    15541 aggcggcccg acaggtaagg acattttcga actgccgttc gttctggttg aaactttctt
    15601 gctgttgttc agctccatca cctacggcat ggcggctatc gccatgtaca aaaacaacaa
    15661 aagccaggtt atctcctggc tggcgttgac ctggttgttt ggtgccggat ttatcgggat
    15721 ggaaatctac gaattccatc acctgattgt taacggcatg ggtccggatc gcagcggctt
    15781 cctgtcagcg ttctttgcgc tggtcggcac gcacggtctg cacgtcactt ccggtcttat
    15841 ctggatggcg gtgctgatgg tacaaatcgc tcgtcgcggc ctgaccagca ctaaccgtac
    15901 ccgcatcatg tgcctgagcc tgttctggca cttcctggat gtggtttgga tctgtgtgtt
    15961 cactgttgtt tatctgatgg gggcgatgta atgagtcatt ctaccgatca cagcggcgcg
    16021 tcccatggca gcgtaaaaac ctacatgaca ggctttatcc tgtcgatcat tctgacggtg
    16081 attccgttct ggatggtgat gacaggagct gcctctccgg ccgtaattct gggaacaatc
    16141 ctggcaatgg cagtggtaca ggttctggtg catctggtgt gcttcctgca catgaatacc
    16201 aaatcagatg aaggctggaa catgacggcg tttgtcttca ccgtgctaat catcgctatc
    16261 ctggttgtag gctccatctg gattatgtgg aacctcaact acaacatgat gatgcactaa
    16321 gagcggcggt tatgatgttt aagcaatacc tgcaagtaac gaaaccaggc atcatctttg
    16381 gcaacctgat ctcggtgatt gggggattcc tgctggcctc aaagggcagc attgattatc
    16441 ccctgtttat ctacacgctg gttggggtgt cactggttgt ggcgtcgggt tgtgtgttta
    16501 acaactacat cgacagggat atcgacagaa agatggaaag gacgaagaat cgggtgctgg
    16561 ttaaaggcct gatctctcct gctgtctcgc tggtgtacgc caccttgcta ggctttgctg
    16621 gctttatgct gctgtggttt ggcgcgaatc cgctggcctg ctggctgggg gtgatgggct
    16681 ttgtggttta tgtcggcgtt tatagcctgt acatgaaacg ccactcggtc tacggcacat
    16741 tgattggttc gctctccggc gctgcgccgc cggtgattgg ctactgtgca gtaaccggcg
    16801 agttcgatag cggcgcagcg atcctgctgg ctatcttcag cctgtggcag atgcctcact
    16861 cctatgccat cgccattttc cgctttaagg attatcaggc ggcaaacatt ccggtattgc
    16921 cagtggtaaa aggcatttcg gtggcgaaga atcacatcac gctgtatatc atcgcctttg
    16981 ccgttgccac gctgatgctc tctcttggcg gttacgctgg gtataaatat ctggtggtcg
    17041 ccgcggcggt gagcgtctgg tggttaggta tggccctgcg cggttataaa gttgctgatg
    17101 acaggatctg ggcgcgcaag ctgttcggct tctctatcat cgccatcaca gccctctcgg
    17161 tgatgatgtc cgttgatttt atggtgccgg actcgcatac gctgctggct gctgtttggt
    17221 aacacaacct ctctattaaa aaggtgctac ggcacctttt ttattgtctg agatcagaga
    17281 tacggctaat aaccgtatgt cttgacagag tgagcagatg aacataaatt aatcatacgg
    17341 ttgatagccg tattatgtat gagatcgact ccctacgatt tcattcagtt aatcaaagag
    17401 gtcataatgc ctggaaaaac agccactcta cccgatgtcg ataaaacgct gaaaaatgcg
    17461 cgggtcgaac tcaaaactag tcccgacgcc aaaaacaaac ttcgtgaagc tgcgcaagcc
    17521 gttggggtag atttaagcgc ttttattttg agcgctgcta tggaacgcgc cgaaagtgtg
    17581 cttgataacc aacgtcgccg tgagctttcg aatcaaagct gggaactgat gaaccaactc
    17641 atcgctgaac ctgctcaacc gacgctcgcc ctcaaggcgt taatgaaaag gaaaaacagc
    17701 gatggtcgac aagcatgaag agattactct gcccatagtc ctctcctgta attatcagtc
    17761 tgatattact tatcctgggc aaaaacagtt tgattgcggt aaccctgtta tcgataaatt
    17821 tgtacgcgca tcgctaaaga aaagtgtgcg taatagcgac tgtgcggcta aagcacttat
    17881 tgacagacaa agtggtgaac tgatcggcat ctgtactttt acggcatatt cgctggaaaa
    17941 acaacgcgtt tctggcgtcc ttcagggttc acaaccttca gaaattggtg ttgtcagatt
    18001 agtcatgttg ggggtagcac ggaagtatca aaagcggggc tttggtcagg acctactatg
    18061 tgattttttt gaacatgtaa aaataattca ccaggcatta ccaattaaag gggtttatct
    18121 tgatgctgac cctgccgcca ttaattttta tgctcgtctc ggctttgttc agctttcagc
    18181 gacaccaaat gcttttggtg ctgtacctat gtttttggcg attcagcata ttctcgcggc
    18241 ttagattatt atcgccaccc gccagggcaa gttttgcctt agccgggggc tgagcagcgt
    18301 taccactttt gttcaattgc ccccgctttc agcaactcca catccttgcc gttaatggtt
    18361 gccgttgcgg caacgattgt ggtccttaca gttgcacaag attttcgctc aaattccggt
    18421 ttataaagaa taagagacga attactggta aatgaaccaa ataggtaatc agtaaagcta
    18481 atattcagcc ggtttaaacc gaaaggcgcg aagccaaccg tttggttaat ctccttaccg
    18541 gatgggcgac gaatgctgaa tgtcacgcga agattggcca cttcgcctaa tttattggtg
    18601 gtggcgaaga ggctatccat gcaggaaaaa cctttatcta aaaaattatc tcgcatccca
    18661 gaacgggtaa ccgtaatcac gggtaacgta tcgtcaaccg tttttctgtc aatcatgtct
    18721 tttcgacaag tagccagaaa gtcttccggg ccaccataaa tgtggacacc tttatcaaga
    18781 tattccttcg ctttcttaca atctacctca acatactgac cattggcgta cataatgccc
//
LOCUS       DAZSBF010000068        18526 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZSBF010000068 DAZSBF010000000
VERSION     DAZSBF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.069"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1713
                     /locus_tag="ACHXWD_001860"
     CDS             <1..1713
                     /locus_tag="ACHXWD_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR7620792.1"
                     /translation="AEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSIST
                     KLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGP
                     WGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGE
                     DGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWA
                     QEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLT
                     MTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAE
                     QSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFG
                     DELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDA
                     LYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARN
                     EMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTG
                     MQVK"
     gene            1714..3363
                     /locus_tag="ACHXWD_001861"
     CDS             1714..3363
                     /locus_tag="ACHXWD_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR7620793.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3368..4144
                     /locus_tag="ACHXWD_001862"
     CDS             3368..4144
                     /locus_tag="ACHXWD_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR7620794.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4218..5402)
                     /gene="atoB"
                     /locus_tag="ACHXWD_001863"
     CDS             complement(4218..5402)
                     /gene="atoB"
                     /locus_tag="ACHXWD_001863"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR7620795.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5433..6755)
                     /locus_tag="ACHXWD_001864"
     CDS             complement(5433..6755)
                     /locus_tag="ACHXWD_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR7620796.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6752..7402)
                     /gene="atoA"
                     /locus_tag="ACHXWD_001865"
     CDS             complement(6752..7402)
                     /gene="atoA"
                     /locus_tag="ACHXWD_001865"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR7620797.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7402..8064)
                     /gene="atoD"
                     /locus_tag="ACHXWD_001866"
     CDS             complement(7402..8064)
                     /gene="atoD"
                     /locus_tag="ACHXWD_001866"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR7620798.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8260..9645)
                     /gene="atoC"
                     /locus_tag="ACHXWD_001867"
     CDS             complement(8260..9645)
                     /gene="atoC"
                     /locus_tag="ACHXWD_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR7620799.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9642..11468)
                     /gene="atoS"
                     /locus_tag="ACHXWD_001868"
     CDS             complement(9642..11468)
                     /gene="atoS"
                     /locus_tag="ACHXWD_001868"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR7620800.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11635..14484
                     /gene="rcsC"
                     /locus_tag="ACHXWD_001869"
     CDS             11635..14484
                     /gene="rcsC"
                     /locus_tag="ACHXWD_001869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR7620801.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14684..15334)
                     /gene="rcsB"
                     /locus_tag="ACHXWD_001870"
     CDS             complement(14684..15334)
                     /gene="rcsB"
                     /locus_tag="ACHXWD_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR7620802.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15351..18023)
                     /gene="rcsD"
                     /locus_tag="ACHXWD_001871"
     CDS             complement(15351..18023)
                     /gene="rcsD"
                     /locus_tag="ACHXWD_001871"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR7620803.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4536 c         4868 g         4596 t
ORIGIN      
        1 gctgagatgc gccagacgct gacgctgcac aaaggggcga attatatttc gctggcgcag
       61 aacattcagc aatctggctt gttaagcgca gaactgcaac aaaatgggca agtgcaggac
      121 agcattagca caaaactgtc ttttgtggat aacagctggc ccgttgaaca gcagaaaaat
      181 gtcatgctcg gcggtggcga gaacgcgctg atgttgcccg agcaggctag caatatccgg
      241 ctacaaagta gcgaaacgcc gcaggagatt ttccgcaaca atcttgatgc gttagtcgat
      301 ggaccatggg gtggcgtgat caacaccggt agccgtctga tcccgctcag tctcgcctgg
      361 cgttcgcttg ccgatcatca aagtgccgcc gctaacgaca ttcgtcagat gattcaggat
      421 aaccgtctgc ggctgatgca actggcgggg cccggagcgc gctttacctg gtggggtgaa
      481 gatggcaatg gtgacgcctt ccttacggca tgggcatggt acgccgactg gcaggccagc
      541 caggcgctcg gcgtaacgca acaaccggaa tactggcagc atatgctcga cagttatgcc
      601 gagcaggcgg ataacatgcc gttattgcat cgggcgctgg tgctggcgtg ggcacaggag
      661 atgaatctgc cgtgcaaaac gttgttgaaa gggttggatg aagctatcgc ccggcgcgga
      721 actaaagatg aagatttctc tgaggaagac atccgcgata tcaatgacag cctgatcctc
      781 gatacaccgg aatctccact ggcagatgcg gtggcaaacg tcttaaccat gacgttgctg
      841 aaaaaagcgc agttgaagtc cacggtgatg ccacaggttc agcaatatgc gtgggataaa
      901 gcggcaaaca gcaatcagcc gctggcgcac acggttgtgc tgctcaatag cggtggtgac
      961 gctacccaga cggccgctat tttaagtggt ttgaccgctg agcaatccac tattgagcgc
     1021 gcgctggcca tgaactggct ggcgaaatat atggcgacaa tgcctccagt tgttttgcct
     1081 gcgcctgcgg gcgcatgggc taaacataag ttaactggag ggggcgaaga ctggcgttgg
     1141 gttggtcagg gtgtgccgga cattctctct tttggtgacg aattatcgcc gcaaaatgtg
     1201 caggtccgct ggcgtgaacc ggcaaaaacg gctcaacaaa gtaacattcc ggtgaccgtt
     1261 gaacgccagt tgtatcggct tatccccggt gaagaagaga tgagctttac tctgcagccg
     1321 gtgaccagca atgagattga cagcgatgcg ctgtatctcg atgaaattac gcttaccagc
     1381 gagcaggatg cagttctgcg ctatggtcag gtggaagtac cgctcccgcc gggagccgac
     1441 gttgagcgca caacatgggg catttcagtc aataaaccca acgccgcgaa acagcagggg
     1501 caattgctgg aaaaagcgcg taatgaaatg ggcgaactgg cctatatggt gccggtgaaa
     1561 gaactgacgg gaacggtcac tttccgccat ttgctgcgct tctcgcaaaa agggcaattc
     1621 gttctgcctc ctgctcgtta tgtgcgttcc tatgcacctg cgcagcaaag tgttgcggca
     1681 gggagtgaat ggaccgggat gcaggtgaaa taagtgaact ggcgcagaat cgtctggcta
     1741 ttggcgttgg ttactctgcc aacgcttgcg gaggagacgc cgttacaact ggtgctgcgc
     1801 ggtgcgcagc acgatcaact gtatcagctg tcgtcatcag gcgtgaccaa agtctcagca
     1861 ttacctgact cgctcaccac gccgttaggg agtttgtgga aactgtatgt ttacgcctgg
     1921 ctggaggata cccatcaacc ggagcagccc taccagtgtc gcggaaactc cccggaagag
     1981 gtttattgtt gtcaggctgg ggagagcata acgcgcgata ccgcgctggt acgttcctgt
     2041 gggctctatt ttgcgccgca gcggttgcat attggcgcag atgtgtgggg gcaatactgg
     2101 caacagcgtc aggcacctgc gtggttagcg tctcttacaa cgctgaagcc agaaacctcg
     2161 gtaacggtta aatcgctgct cgattcatta gcgacgttac ctgcgcaaaa caaagctcag
     2221 gaagtcttac tggatgtggt gctggacgag gcgaaaatcg gtgtcgcatc catgctcggt
     2281 agtcgggtac gggtgaaaac ctggagctgg tttgccgatg ataaacagga aatccgccag
     2341 ggtggttttg ctggctggtt aaccgatggt acaccgctgt gggttactgg cagtggcacc
     2401 agcaaaacgg tactaacccg ttatgcgacg gtgcttaatc gggttttgcc agtgccgacg
     2461 caggttgcca gtgggcaatg tgtagaggtg gagctgtttg cccgctatcc gctgaagaaa
     2521 atcacagcgg aaaagagtac gacggcagta aaacccggcg tacttaatgg gcgctatcgt
     2581 gtgacgttta ctaatggtaa ccacattacg tttgtcagcc acggtgagac gaccttactg
     2641 agcgaaaagg gcaaactgaa attgcagtcg catctcgatc gtgaagagta cgttgccaga
     2701 gttctggatc gcgaggcgaa aagtacgcca cctgaggcag cgaaagccat gaccgtggcg
     2761 atacgtactt ttttacagca aaacgccaat cgggaaggtg attgcctgac catccccgac
     2821 agcagcgcca cgcagcgtgt ttctgcttcg ccagcgacca cgggggcgcg aacgatgacc
     2881 gcttggacgc aggatctcat ctatgcaggc gatccggttc attatcatgg cagtcgcgct
     2941 accgaaggga cactttcctg gcggcaagcc acggcccagg cggggcaggg ggagcgttac
     3001 gaccagatac tcgcgtttgc ttatcccgac aacagcctta gccgctgggg agctccacgc
     3061 tcaacctgcc agttattacc caaagcgaaa gcctggctgg cgaaaaaaat gccgcagtgg
     3121 cggcgtatat tacaaggcga gacggggtac aacgaaccag acgtgtttgc ggtctgtcgt
     3181 ttagtctcag gtttccccta taccgatcgg cagcagaaac ggctgtttat tcgtaacttc
     3241 tttacgcttc aggatcggct ggatttaacc catgagtatc tgcacctggc ctttgatggt
     3301 tatcccaccg ggcttgatga gaactatatc gaaacgctga cccgacaatt attaatggac
     3361 taatgctatg cgaaagattt ttcttccgtt gttactggtg gcactttcgc ctgtcgctca
     3421 cagtgaaggt gtgcaggaag tcgagattga cgcaccgctc tctggctggc atccggcaga
     3481 gggcgaagat gccagttttt cacaatcgat caattacccg gcgtcgtccg tcaacatggc
     3541 agatgatcaa aatatctcag cgcagatccg cggcaaaata aagaattatg ctgcggcggg
     3601 taaagttcag cagggccggc tggtggtcaa cggtgccagt atgccgcaga gaattgaatc
     3661 cgatggttca tttgcacgcc cttatatttt cactgaaggc agcaacagcg tgcaggtcat
     3721 cagcccggat gggcaaagcc ggcaaaaaat gcagttttac tcaacgtcgg gtataggaac
     3781 gattcgtgca cgtttacggc tggttctctc gtgggatacg gacaataccg atctcgatct
     3841 ccatgttgtc acgcccgatg gcgaacacgc ctggtacggt aacaccgtgc tgaaaaacag
     3901 tggtgcactg gatatggatg tcacgacggg gtacggaccc gagattttcg ccatgccagc
     3961 accagttcac gggcgctatc aggtgtatat caactactat ggtgggcgca gcgaaacgga
     4021 attgacgacc gcccaactga cgctgatcac cgatgaagga tcggtcaatg agaaacagga
     4081 aacatttatt gtaccgatgc gtaatgctgg cgagctgacg ctggtgaaaa gttttgactg
     4141 gtgattaatg gggttacctt cgccgtcgat gttcaagaaa acgcccgata actttcgcta
     4201 tcgggtgttt ttattgatta attcaaccgt tcaatcacca tcgcaattcc ctggccgcca
     4261 ccaatgcaca gtgttgccag ccccagcgtt ttatcgcgtg cctgcatggc atgtaatagt
     4321 gtgaccagaa tacgagcacc actggcaccg ataggatgcc cgagcgcgat ggccccgccg
     4381 ttgacattca ctttctcaga atcaaagccc agggttttcc caacggcgag gaactgtgca
     4441 gcaaatgctt cattagcttc gatgagatca atgtctgcca gttgcagccc cgccagttgt
     4501 aacgcttttt gcgtggcagg tactggcccc atacccatca atgcgggagg cacgccaccg
     4561 ctggcataac ttttaatgcg agccaggggg gtaaggcctg ctgccagcgc cgcagattct
     4621 tccataatca ccagagcggc agcaccgtcg ttaataccag acgcgttccc agcggtgact
     4681 gttcctgctt tatcgaaggc cgggcgcaat gcacctaacg cttcagccgt tgaattcgct
     4741 ttcgggaatt cgtcttgact gaagacgaag gttttcttcc gagtgacaac atttaccggg
     4801 acgatttcgg ctgtaaaagc accggactca attgcggctg ccgctttacg ctgtgaatgt
     4861 agcgccagtt catcctgcat ttcacgggta attccgtact ctttagccac gttttcggcg
     4921 gtaatcccca tatgataacc atgggtggcg cacatcaggc catcgcgcag gattacgtca
     4981 taaacctgtc cgtctccaag acgataacca gagcgtgctt ttgcatcgag taagtagggc
     5041 gctaaactca tattttccat accccccgcc acaatgctct tcgcctgacc tgcctgaatt
     5101 gcctgggcgg caagcgccac acttttaaga cccgaaccac acactttatt gaccgtgaat
     5161 ccgcacaccg tttctgccag cccgcttttt aacagtgcct gacgtgccgg attttgcccc
     5221 aacccggctt gcaacacgtt acccataatc acttcatcaa tgtgtagtga atcgattttt
     5281 gcacgttcaa tggcggcttt aattactgtc gcccccaggt cgatggcgct ggtggaagcg
     5341 agtgaaccgt taaaactacc gatagcagta cgtaccgcac tgacgatgac acaatttttc
     5401 attttatatt ccttctgtct gtagggttgc cgtcagaaca gcgttaaacc aatgacgaaa
     5461 atgacgccgg agaagagcag ggcagtgatg caatagccca tgatgtcgcg gacaccgagt
     5521 ccggcgattg ccagtgctgg cagcgcccag aatggttgtt ccatgttcat ccattgctcg
     5581 ccgtaggcga tcgccattac cgatttaccg agatcagcgc ccagcgcctg ggctgcgggt
     5641 atcacgaaag gtccctgaat aacccagtga ccgccgccag acggaacggc gaagttaatc
     5701 agtgcagaac taaaaaaggt cattaccggg aaggtgtctt tgttcgcaac attgatgaag
     5761 aattcggtaa tgagtccacc cagaccagaa tgctccatca tcagttggat cccagcgtag
     5821 aaggggaatt gcaccagaat accggcagta ctgcgtgctg ccgcgctgat agcacgcata
     5881 taagccattg gcgttttatg tagcaacaga cccgcaatca taaacatcag gttgacggta
     5941 ttgatggtga tgttgaagcc atgttcgctg aagtacatcg ccaggtaagc gataccgagt
     6001 gcgccgatga tcaacgtcag aatgcggctt tcttccagtc gctcggatgg tggggcatct
     6061 ttcggtagct gcttttgaaa atcagcctct tccatgagta gttccggatc gatactcacc
     6121 acgtcagacg gttttggcat catcatgcgg gtgataaatg gcatcaccac aatcaacgtc
     6181 acagtgatga aaatgttaaa accactgaac agagtatcgc ccaccgggat cagcccggcg
     6241 atatgctcaa ccgggttgcc cggtgttgca gccaacagag gcattgatcc agagaagccg
     6301 ccaccccagg tgagaaaacc aatgtaggcg caggcaatga gcaacggata atcagaaccg
     6361 gggactcgcc gggcgacttc acgggcaaac attgcgccga caaccaaacc aaatccccag
     6421 ttgatgacac aagcgactga accgaagaaa gtgaccagca tgacgccctg tacgggcgtc
     6481 tttgcggcgg aggcggcagt acgcagcaaa cttttcaccg gagcagagct ggcaagggca
     6541 tgaccggtaa cgatgataag cgccatctgc ataccaaacg ccagcaagtt ccagaaaccg
     6601 tcaccccaca ttttcaccat gctgatcggc gtttgtggtg ttaaccaaag cgcgatcacg
     6661 aatgttagca atgtcagcaa catggcaaag atcagtggat cgggaagcca tcggctgaca
     6721 aaacgcgtca taaaacgcga tatgcgacca atcataaatc accccgttgc gtattcagat
     6781 cggcggcgac ttcaaaccga gcttctgttt tggcacgcac ggtggctaaa tcacacccgt
     6841 cggcaatttc ggtgagccac attttgccgt caataaaacg aaagacagcc agttcagtaa
     6901 ccagcatatg caccgcatgt tgcgcagtga gtggcatggt gcagctgcgc aaaatttttg
     6961 ctgaaccatc tttggcgcaa tgttccatgg cgatgatcac tttgcgcgac ccggtcacca
     7021 gatccatcgc gccacccata ccgggcacca ttttcccagg cactacccag ttcgcgaggt
     7081 ttgcttcttc gtctacttgc aaaccgccga gcacgcaggc atcaatatga ccgccacgga
     7141 ttagcgcaaa tgacatggcg ctatcaaaca tggctgcacc gggtaaaaca ccgcacggtt
     7201 gcccgccagc gttcaccaga tctggatgcg ctgtcgtgac cgggcctaaa ccgaggaagc
     7261 cgttttccga ttgcagagtg atatgaatac cctccggtaa ataattggcg accattgtgg
     7321 gtaaaccgat ccctaagtta acgatgtcac catcacgaag ctcttgcgcc acacggcgcg
     7381 caatacgttg tttcgcatcc attatttgct ctcctgtgaa acgatgatgt ggtcgataac
     7441 ggcaccaggg gtgacaatat ggtcaggttg cagctcgccg gtttcgacca gttcatctgg
     7501 ctctaccagc gtgatatcag ccgcaagggc tatcaggggg ttaaagttgc gggcgctaag
     7561 ttgataggtc aggttgccaa gtgtgtcgca acgatgagcg cgaattagcg ccaggtcggc
     7621 gcgcagtggg cgttcgagca gccaggtttt accgtcgagt gtcagtgtct gtttgccttc
     7681 ctctacgacg gtgccgacac ccgttggggt gagaaaacca ccaagtccag ctccaccaca
     7741 gcgaatttgc tcgattagcg taccttgcgg caccagaacg acgtccatct caccagatat
     7801 catgcgccga cctgtttccg ggttggtgcc gatatgtgaa gcaatcactt tgcggactcg
     7861 accattgacg atgagcggac cgatgccggt atcaacaaac gcggtatcat tggctatcaa
     7921 tgtcaggtcg cgaacaccag attccagtaa tgcttcaacc aggcgggatg gagtgccaat
     7981 ccccataaat ccgcccacca tgatggtcat gccgtcacga aagaagccgg tggcgtcttg
     8041 taatgtcatc aattttgttt tcatttttta tccctcttgc atacaccgtt gtgggtacag
     8101 tcaggcaata gcaaggggag tgccagaatg agattattgg atttagttat ttaactaatt
     8161 gaatatgaag ggataaattg gatggggatt tataaggaga atgagcggct atgcagaaaa
     8221 ttgcgcactg tgcaaatttc tgcatagcaa gttttggtgt tatacatccg ccggatcgat
     8281 accgtattcc tggagtttat acatcaatgc acggcgactg atgcccagca ttaaagcagt
     8341 gcgggttcgg tttccttctt gttgttccag cacttccata atgatgcgtt tttcgacgcg
     8401 tttaatttcc tcttttaaat tacgctcacc gacagaggct gtttttacct cgccagcatt
     8461 acagactggc tgacgaatct gtggcggaag atcctcagaa aaaatgatcg ggcctgaatt
     8521 catcacgacg gcgcgttcaa taacgttaga aagctctcga atatttcccg gccatgacca
     8581 ggcggtaagc agtgacattg ccatcggatc gatgtcgata atatcgcgct gattctcact
     8641 actgaatttt tgcaaaaagt gattagctaa cagggaaata tcttcccggc gatcgcgcag
     8701 aggcggcagt attaaatgaa taacgttaag gcgataaaag agatcttcac ggaaggtgcc
     8761 ttcttttacc attgcctgca agtcacggtt ggtggcagca atgatgcgga tatcaacttt
     8821 tatggtctga tggccgccaa tccgttcaaa ttcccgttcc tgtagaatgc gtagtaattt
     8881 ggcttgtagt accagcggca tttcgccaat ttcgtcgagg agcagagtac cttcgttggc
     8941 tcgttcaaat aatccctgac gcaaggtttg tgcaccagta aatgcacctt tttcatgacc
     9001 aaacagttca ctttcgagca acgattccgg cagcgccgcg cagttgactt taatgaacgg
     9061 cccctttgcc cgccgcgaat tgtagtgaat cgctctggca atcaactctt tcccggtgcc
     9121 gctttcaccg ctaatcaaga cgctggcctg agaaagggca attttggcgg tgtctttgca
     9181 gatgtccatc atcgccgggc tgttggtgag aatgtgcccc cattgccagc tggcgctcag
     9241 tgcctggtgc agatgacgga tctctttttt cattgactgg agttgtaaag cgcgctgaac
     9301 gattaaattc aactcatcga gatcaaacgg tttaataaca tagtcgaagg ctccgcagcg
     9361 tagcgcttcg acggcggttt ccacttccgc ataggccgtc atcagaataa cgggtgtccg
     9421 ggtctcatgg ctgcgcatct cctttagtgc cttgatgccg tccatctctg gcatgcggat
     9481 atccatcaac accacatcag ggtgaatatc ggcaaacagg tgtaatgctg tgcgtccgtt
     9541 gttcgcacaa tgtgtttcga atccttgtag tgcaaaagcg gtgctcagca tacggcgaac
     9601 attatcttca tcatccacaa taaggatgcg attaatagca gtcatacagt ctgatttccc
     9661 tgcgggttga tcggtaaaat aagcgtgaag gttgcgccgt agcccggcaa actggcgacg
     9721 cgaatatcac cctgatgggc attaatgatg cgttgactta acgccagacc aagcccggtt
     9781 cctgaggctt tggtggtgaa aaagggatcg aagatctttt tttgcagcga gagatcaatg
     9841 ccacagccgt tgtcctctat cgaaatggcc tgttgtgagt cgctgtattg ccaggtttga
     9901 atgcgaattt tccctcgtgc gctgatagcc tggacggcat tgatcaggat attcagtagt
     9961 acctgtttga gcagttcacg atcggcgtta atcgggctta attcattatc cagttcgctt
    10021 atgaagtcga cccgcgcttg tacgccggcg gtttgtacca gtaccagagt ttcttcaacc
    10081 aatgcattga ggctgacttg ttgccattga ctgtggcgtg gacgtgaaaa ttcgagcaat
    10141 tgctgaataa ctttgttaat tgaatcgatt tctttgagta ctacggacag atattcctga
    10201 tgtattgggt cactggtttg ttggcgcaag atctgtacat aaccacgaat agccgttaac
    10261 ggattacgta cttcatgcgc gacgccagcc atcagctcac ccagtgtggc gaggcgttct
    10321 gcttgcgcca tgcggcgctg ggtttctttg cgggcagtta aatcagagaa aatcaccaaa
    10381 gcacctatca tttcaccgtg cgtgttatga atacgactgg tagtgacact gagttcaatg
    10441 gtgcggtcac gacctggaaa actgatctcc agcgccacat gttcggtgcc atgttccagc
    10501 gtatccagta ctggactgta gaactgagta ttgtcgaaca acatggagta aggctgccct
    10561 accagttcat ggcgttgata gccagtgata acttctgctg ctgggttcat ggtggttaca
    10621 tcaccctggc ggtcaatggc aatgacgcca tcggcagcgt tttcaataat cagatcgtta
    10681 agtgtccgcg tttcacgcag tgcctgggcg aggttattaa cactctgact gatttgcccc
    10741 atttcaccgg gcaattgtgg taatcgagtg ggaatatttt gtgccagagt cgagaggcca
    10801 tcggtgatga tatcaatatt ggcgctcagg cgacgggaga aaaggacaat caatagcagg
    10861 cttatcagca aatcggcggt gagcacaatg ataatcctca catccatttt ccaggcctgg
    10921 cggcgaatat cttcggttaa ttcattggcc cagatatagc cgaggatttc accattacgc
    10981 tcaatgggga gcattgaatt caaaatatcg ccgcgcacct gcctgcctga ataaaccaaa
    11041 ggggtatttg tacgcatgac ttcgcgacca gggtgatctg cggcaatggt aacgccgaca
    11101 ttattctgat atagcgctga aggcgcgtag gtgattatcg catccagcat tttgttgtaa
    11161 taaccagcac ctatgccagg gaaggcgtga gtgatatttt cggtaatggg ggcaagttct
    11221 gcatttaatg cgcggatacg ctcctcacgt ggtaagtcga tgtagagatc atagcgatcg
    11281 cctagtgcct gattaagcag gttgaccacg gcagaaagtt ttttctcttt ttcagataag
    11341 actgctgaac gtccttccgt ttctacgata taaccaatag taagcgttgg gacaatgacc
    11401 atcaggattg ccatcaggat catttgattg cgtaagcggc gtggataaat ccacttcata
    11461 taatgcatgc tgaaatccac tagtcttgtc cggtatatga cgattatcag aggttaaggt
    11521 gatgatttct cggcggtgta tcatattcca gagaagagag aacattgcgg taacacgctt
    11581 ttaccgctac cttaaccaca ctccatcggt cacctgaggc ggagcttcgc ccctttgaaa
    11641 taccttgctt cttttcgtac aaccctgaaa gcctcgcgct acatgttcag agcattggcg
    11701 ttagtgctct ggctgttgat tgctttttca tccgtttttt acatcgttaa tgcgttacat
    11761 cagcgagaat cggaaattcg tcaggaattt aatctgagtt ccgatcaggc tcagcgcttt
    11821 attcaacgca cctctgatgt gatgaaagag ctgaagtaca tcgccgaaaa tcgcttatcg
    11881 gcagaaaacg gtgtgctttc cccgcgtgga cgagaaacgc aggcggatgt gcctgcgttt
    11941 gaaccgctgt ttgccgactc cgattgttcc gcaatgagta acacctggcg aggttctctg
    12001 gagtcattgg cgtggtttat gcgctactgg cgcgataatt tttctgcggc ttacgatcta
    12061 aaccgggtat ttttaatcgg cagcgataac ctctgcatgg ccaatttcgg tctgcgtgat
    12121 atgccagtgg aacgcgatac cgcgttgaaa gctttgcatg aacgcatcaa taaatatcga
    12181 aatgcaccac aagatgatag cggcagtaac ctctactgga tcagcgaagg tccgcgccct
    12241 ggcgtcgggt atttttacgc gttgacgcca gtttatctgg cgaaccggtt gcaggcgctt
    12301 ttgggtgtcg agcagaccat ccggatggag aactttttct taccgggtac gttgccgatg
    12361 ggggttacca ttcttgatga aaatggtcat accctgattt cgcttaccgg accagaaagt
    12421 aaaattaagg gcgatcctcg ctggatgcag gaacgctcct ggtttggcta tacggaaggg
    12481 ttccgggagc tggtgctgaa gaaaaatctg ccaccctcat cgctaagcat cgtgtattcg
    12541 gtgccggttg ataaggtgct ggaacgcatt cgcatgttga tccttaacgc aattttgctg
    12601 aatgtgcttg ccggagctgc attgtttact ctcgcacgga tgtacgagcg acgtattttc
    12661 attccggcgg aaagcgacgc cctgcgactg gaagaacatg agcagttcaa tcgcaagatt
    12721 gtcgcctccg cgccagtggg tatctgcatt ttgcgtaccg ctgatggcgt caatatttta
    12781 agtaacgaac tggcgcatac ctatctcaat atgcttacgc atgaggaccg ccaacgactg
    12841 acgcaaatta tctgtgggca gcaggtcaat tttgttgatg tcctgaccag caacaatacc
    12901 aatctgcaaa tcagcttcgt ccattcgcgc tatcgtaatg aaaacgtggc catttgtgtg
    12961 ctggtggatg tttcttcgcg cgtgaagatg gaagagtcgt tgcaggagat ggcacaagca
    13021 gcggaacagg cgagccagtc aaaatcgatg ttccttgcca ccgtcagtca tgagctgcga
    13081 acgccgctgt atggcattat cggtaacctg gatctgttgc aaaccaaaga gttaccgaaa
    13141 ggcgtcgatc ggctggtgac ggcaatgaac aactcttcca gcctgttgtt gaaaattatc
    13201 agcgatattc tcgatttctc gaagattgaa tcggaacagt tgaagatcga accgcgtgag
    13261 ttttcaccgc gtgaagtgat gaaccacatc accgccaact atttaccgct ggtggtacgc
    13321 aagcagttag gcttgtactg ctttattgaa ccggatgtgc cagtggcctt aaatggcgac
    13381 ccgatgcgtt tacagcaggt catctccaac ctgttgagta acgccataaa attcaccgat
    13441 accggctgta tagttttgca tgtccgcgct gatggcgatt atctctctat ccgtgttcgc
    13501 gataccggcg tggggattcc ggcgaaagaa gtggtgcgct tgtttgatcc cttcttccag
    13561 gtcggaacgg gcgtacagcg aaacttccag gggaccggtc tgggtctggc gatttgtgaa
    13621 aaactgatca gcatgatgga cggcgatatc tcggtagatt cagaaccggg aatgggcagc
    13681 cagtttaccg tgcgtattcc gttgtacggc gctcagtacc cgcagaaaaa aggcgtggaa
    13741 gggttgagtg gtaaacgctg ctggctggcg gtccgcaatg cgtcgctctg tcagttcctg
    13801 gaaaccagtt tgcagcgcag cggcatcgtc gttacaacat acgaagggca ggaaccgact
    13861 cccgaagatg tgttaattac tgacgaggta gtgagtaaaa aatggcaggg tagagcggta
    13921 gtgaccttct gtcgtcgcca tattggtatt ccgctggaga aagcgccagg ggagtgggta
    13981 cacagtgtgg ctgccccgca tgagctaccg gcattgttgg cgcgtattta tttgatcgag
    14041 atggagagcg acgatcctgc taacgctctg ccgtcgacgg acaaagcggt cagcgataat
    14101 gacgatatga tgattctggt cgtggatgat catccgatta accgacgtct gctggcagat
    14161 cagttgggat cgttgggcta tcaatgtaaa accgcgaatg atggtgtcga tgcgcttaat
    14221 gtacttagca agaatcatat tgatatcgtg cttagcgacg tcaacatgcc aaatatggat
    14281 ggttaccgct tgacgcaacg cattcgtcag ttgggactga cgttgccggt aatcggagta
    14341 actgctaatg cgttggctga agagaagcag cggtgtctgg agtccggtat ggacagctgc
    14401 ctgtcgaagc cggtaacgct ggatgtgata aaacagacgc tgacggtata tgccgagagg
    14461 gtcaggaaat cgcgggaatc gtaggacgga taaggtgttt atgccgcatc tggcattcag
    14521 tgcaaatgcc agatgcgacg ctgacgcgtc ttatctggcc tactttgatg taatctgttg
    14581 aaataatggg aatcgtagga cgcataaggc gtttacgccg cattcggcaa tcggtgcaaa
    14641 tgccagataa gacactaacg cgtcttatct ggcctacagg tgattagtct ttatctgccg
    14701 gacttaacgt cactgaagag agataattca gcagggcgat atcgttctcg acacccagct
    14761 tcatcatcgc agatttcttc tggctactga tggttttaat actgcggttc agctttttag
    14821 cgatctcggt caccaggaag ccttccgcaa acaggcgcag aacttcactc tcttttggcg
    14881 agagacgctt gtcaccgtaa ccaccagcac tgattttttc caacaggcga gaaacgcttt
    14941 ccggggtaaa tttcttccct ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg
    15001 caccttgttt cagcacgatc ccttcgatat ccagatccaa taccgcacta agaatcgccg
    15061 ggttgttgtt catagtcaga acaatgatcg acaggcttgg gaaatggcgc ttgatgtact
    15121 tgattaaggt aatgccatcg ccgtacttat cgccaggcat ggagagatcg gtaatcaaca
    15181 catgcgcatc cagtttcggc aggttgttga tcagtgctgt agagtcttca aattcgccga
    15241 caacattcac ccactcaatt tgctcaagtg atttgcgaat accgaacaag actatcggat
    15301 ggtcatcggc aataattacg ttcatattgt tcatgtaata ggctaccttg ctacagcaag
    15361 ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca
    15421 cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca
    15481 aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact ggttgctgct
    15541 tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg
    15601 ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc
    15661 gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag
    15721 ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc
    15781 aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta
    15841 attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca
    15901 atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc
    15961 tcttcttcct cttcaacttc cgggccagcg gcgagcattt tgatatgcac agagtagcgt
    16021 gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg tgccagttga
    16081 tcgctcagcc agaatgccag cgggtccgcc tttccataac gatcgttttg ggtctggttg
    16141 ataaacggga agtgcaaatt atccatttca tgaatactta cgccttctcc ggtgtccaga
    16201 atgcggaatg tcaggcggtc ttcagaggac tcatcctgat caacctcaag ggtgattttt
    16261 cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg tcgtaaggca
    16321 tcgcgatcgc cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag cagttgcaga
    16381 cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg
    16441 gagaacagca ccgtctcact tttccagcta tcgtccgcaa gcatgttcgc taactgtatt
    16501 tcatcgacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct ttccggggcg
    16561 ttgagtttag ccgcgctctc cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg
    16621 tttttcataa aggtcatccg gccctgctgg tttttctcat acagacgctg cgcctgcttg
    16681 agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc
    16741 ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga
    16801 ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc
    16861 aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc
    16921 aggccgagcg gcagcagtga gactatcttt tcattgattg cccgtaaaat gcgcaattcg
    16981 ttattgaccg ccgtgttggg tagactttct gtactgcgac tggagaaatg gcggaatgtg
    17041 gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg
    17101 ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccag gcgcatatcg
    17161 gtagagttga gtgccgagga gatttcaatc ttcgtactgt taaagtggat actgacgcta
    17221 tccgttcctt ctttctcatt atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg
    17281 aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccacg
    17341 accgttgcca gatgtcccgg ctggttgaac gtggtacgca aggtaaagta atgaccgttc
    17401 tgccaggcca ggcggcgcat gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt
    17461 tgcaacattt ctgcacgacg tgaatcaaca atgtcactga cggtcgattc tttaaatccg
    17521 gaggtgagat ctttgagggg cagggttgag atcagtacca gactgttatc ctgaccattc
    17581 aggtaataca tcgaccacgg tacattttct gcaccccaca atgtatccag ataagtggac
    17641 atccgctgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt
    17701 ttgcggcgtg gtttctccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct
    17761 tcgccggagg agggggaggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa
    17821 cgccaggtat cgacgcgctt ttggattgcg tgggtaatgt cgacaatctg gtaactttta
    17881 tctttcagcc aggcgttaac ggcgctttgt accattaccc ccatcgtcac cagtaacaca
    17941 atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg
    18001 gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata
    18061 gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat gattctctat
    18121 tctcccgtga tatttatcat cctgcattgg gggaaataga tgtacaagcg ccattttgct
    18181 gtacaaataa cctacaaaaa gcccaacgaa attaattgca aataaacgaa gattgctgga
    18241 aattatgctg atgttaatta tttgtgaaat agttgacaag agttatagtt tttctgtggt
    18301 agcacagaat aatgaaagtg tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg
    18361 ttgaaatagg ggtaaacaga cattcagaaa tgaatgacgg taataaataa agttaatgat
    18421 gatagcggga gatattctag ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa
    18481 atacttaaga ataagttatt gattttaacc ttgaattatt attgct
//
LOCUS       DAZSBF010000069        18515 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZSBF010000069 DAZSBF010000000
VERSION     DAZSBF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.070"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="ACHXWD_001872"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="ACHXWD_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR7620804.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="ACHXWD_001873"
     CDS             737..1042
                     /locus_tag="ACHXWD_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620805.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="ACHXWD_001874"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="ACHXWD_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR7620806.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="ACHXWD_001875"
     CDS             1790..2536
                     /locus_tag="ACHXWD_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR7620807.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="ACHXWD_001876"
     CDS             2536..4632
                     /locus_tag="ACHXWD_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR7620808.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="ACHXWD_001877"
     CDS             4678..5817
                     /locus_tag="ACHXWD_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR7620809.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="ACHXWD_001878"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="ACHXWD_001878"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR7620810.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="ACHXWD_001879"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="ACHXWD_001879"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR7620811.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /gene="appA"
                     /locus_tag="ACHXWD_001880"
     CDS             complement(8566..9864)
                     /gene="appA"
                     /locus_tag="ACHXWD_001880"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR7620812.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="ACHXWD_001881"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="ACHXWD_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR7620813.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="ACHXWD_001882"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="ACHXWD_001882"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR7620814.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="ACHXWD_001883"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="ACHXWD_001883"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR7620815.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="ACHXWD_001884"
     CDS             complement(12875..13732)
                     /locus_tag="ACHXWD_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR7620816.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="ACHXWD_001885"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="ACHXWD_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR7620817.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /gene="hyaD"
                     /locus_tag="ACHXWD_001886"
     CDS             complement(14124..14711)
                     /gene="hyaD"
                     /locus_tag="ACHXWD_001886"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR7620818.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="ACHXWD_001887"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="ACHXWD_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="ACHXWD_001888"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="ACHXWD_001888"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR7620819.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="ACHXWD_001889"
     CDS             complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="ACHXWD_001889"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR7620820.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4544 a         4913 c         4783 g         4275 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 tatcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg tgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc
    17701 aagcgatcaa aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg
    17761 cagccaggta ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc
    17821 gtcggattgg gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata
    17881 atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg
    17941 atacagaaca tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg
    18001 ccattgtatt gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc
    18061 atcaaagtat cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg
    18121 tgagcggagc ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc
    18181 accggaatgc ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct
    18241 aatcccagcg acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc
    18301 tgacgccgca tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct
    18361 tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc
    18421 aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg
    18481 tcgccagaaa gtattgttaa taaagcgtag tgaaa
//
LOCUS       DAZSBF010000070        18214 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZSBF010000070 DAZSBF010000000
VERSION     DAZSBF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.071"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHXWD_001890"
     CDS             15..1091
                     /locus_tag="ACHXWD_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7620821.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHXWD_001891"
     CDS             complement(1484..1825)
                     /locus_tag="ACHXWD_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR7620822.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHXWD_001892"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHXWD_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR7620823.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHXWD_001893"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHXWD_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR7620824.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHXWD_001894"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHXWD_001894"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR7620825.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHXWD_001895"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHXWD_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR7620826.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHXWD_001896"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHXWD_001896"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR7620827.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHXWD_001897"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHXWD_001897"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR7620828.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHXWD_001898"
     CDS             complement(7167..7826)
                     /locus_tag="ACHXWD_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR7620829.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHXWD_001899"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHXWD_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR7620830.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHXWD_001900"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHXWD_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR7620831.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHXWD_001901"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHXWD_001901"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR7620832.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHXWD_001902"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHXWD_001902"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR7620833.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHXWD_001903"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHXWD_001903"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR7620834.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHXWD_001904"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHXWD_001904"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR7620835.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHXWD_001905"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHXWD_001905"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR7620836.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHXWD_001906"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHXWD_001906"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR7620837.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHXWD_001907"
     CDS             complement(17270..18112)
                     /locus_tag="ACHXWD_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR7620838.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4575 c         4392 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAZSBF010000071        17961 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZSBF010000071 DAZSBF010000000
VERSION     DAZSBF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.072"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(231..1205)
                     /gene="cysB"
                     /locus_tag="ACHXWD_001908"
     CDS             complement(231..1205)
                     /gene="cysB"
                     /locus_tag="ACHXWD_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR7620839.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1415..4012)
                     /gene="topA"
                     /locus_tag="ACHXWD_001909"
     CDS             complement(1415..4012)
                     /gene="topA"
                     /locus_tag="ACHXWD_001909"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR7620840.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4392..4643
                     /locus_tag="ACHXWD_001910"
     CDS             4392..4643
                     /locus_tag="ACHXWD_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR7620841.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4679..5728)
                     /gene="sohB"
                     /locus_tag="ACHXWD_001911"
     CDS             complement(4679..5728)
                     /gene="sohB"
                     /locus_tag="ACHXWD_001911"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR7620842.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5948..6706
                     /locus_tag="ACHXWD_001912"
     CDS             5948..6706
                     /locus_tag="ACHXWD_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR7620843.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6703..7293
                     /gene="cobO"
                     /locus_tag="ACHXWD_001913"
     CDS             6703..7293
                     /gene="cobO"
                     /locus_tag="ACHXWD_001913"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR7620844.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7333..8205)
                     /gene="rluB"
                     /locus_tag="ACHXWD_001914"
     CDS             complement(7333..8205)
                     /gene="rluB"
                     /locus_tag="ACHXWD_001914"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR7620845.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8418..9532)
                     /locus_tag="ACHXWD_001915"
                     /pseudo
     CDS             complement(8418..9532)
                     /locus_tag="ACHXWD_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9560..10180)
                     /locus_tag="ACHXWD_001916"
     CDS             complement(9560..10180)
                     /locus_tag="ACHXWD_001916"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR7620846.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10177..11058)
                     /gene="rnm"
                     /locus_tag="ACHXWD_001917"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10177..11058)
                     /gene="rnm"
                     /locus_tag="ACHXWD_001917"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR7620847.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11196..11240
                     /gene="trpL"
                     /locus_tag="ACHXWD_001918"
     CDS             11196..11240
                     /gene="trpL"
                     /locus_tag="ACHXWD_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR7620848.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11217..11310
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11332..12894
                     /gene="trpE"
                     /locus_tag="ACHXWD_001919"
     CDS             11332..12894
                     /gene="trpE"
                     /locus_tag="ACHXWD_001919"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR7620849.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDNRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12894..14489
                     /gene="trpD"
                     /locus_tag="ACHXWD_001920"
     CDS             12894..14489
                     /gene="trpD"
                     /locus_tag="ACHXWD_001920"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR7620850.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14490..15851
                     /gene="trpCF"
                     /locus_tag="ACHXWD_001921"
     CDS             14490..15851
                     /gene="trpCF"
                     /locus_tag="ACHXWD_001921"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR7620851.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15863..17056
                     /gene="trpB"
                     /locus_tag="ACHXWD_001922"
     CDS             15863..17056
                     /gene="trpB"
                     /locus_tag="ACHXWD_001922"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR7620852.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17056..17862
                     /gene="trpA"
                     /locus_tag="ACHXWD_001923"
     CDS             17056..17862
                     /gene="trpA"
                     /locus_tag="ACHXWD_001923"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR7620853.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4318 a         4750 c         4487 g         4406 t
ORIGIN      
        1 aatccagcga atttacgaat cgtctccagt gaataattca atcttttgac ctgaaataga
       61 tatttgaaaa ctggagtttg cgaggcgggt aattagacac tactttacag gtaatttacc
      121 ctataaatat gagggataaa aatattgtgc acggtataga aataagtaac aaaagagaat
      181 atattccggc accttcgcta cataaaaggt gccgaaaata acgcaagaaa ttatttttcc
      241 ggcagtttta tatctttaaa catgacctca atttcttcat tagagcgcaa tgcgacagcc
      301 gcatcaacga catcacgcgt taaatgcggt gcaaaacgct gaatgaaatc atacatataa
      361 ctgcgcaaga aagtactacg gcgaaaacca attttggttg tactgtggct gaagatatcg
      421 tgagcatcaa cacgcacaag gtcgggatcg gcgaccggat ccaccgccat gctggcaatg
      481 acccctaccc ccagccctaa ccggacgtaa gttttaatga cgtcagcatc cgttgccgtg
      541 aagacgatac gcggcgttaa ccctgcgcgg ttaaaggcag tatccagttc tgaacgtccg
      601 gtaaagccga aggtatatgt caccaacgga tattgcgcca gttcttcaat ggtaatggct
      661 tttttgcctg ccagcgggtg atccggagtg actacaatag cccgattcca gtggtagcac
      721 ggtaacatca ctaaatcttc atacagatgc agcgcttctg tggcgatagt gaaatcagca
      781 ttgcctttag agacggcatc agcaatttgt gtcggcgagc cctggtgcat atgcaaagaa
      841 acgcggggat aacgctcaat aaagcctttg ataacgtttg gtaacgcgta gcgtgcctgg
      901 gtatgcgtgg tggcgatata cagtgagcct ttatccggcc aggtgtgctc tccggcaacc
      961 gattttatgg catcgacttt cgacaggact tcgcgagcga tacgaattat ttcttgccct
     1021 gctggcgtta cctgcgtcag gtgcttgccg cttcgggaaa aaatttgaat gcctagctcg
     1081 tcttccagca ttctgacttg tttactgatc ccgggttgtg atgtgtaaag tccttccgct
     1141 gttgatgaga cattcaggtt atgattgacc acctcaacaa tatagcgaag ttgttgtaat
     1201 ttcatgttaa accatccact tagacgccat cagaacatcg ttttttcgtc acacctggcg
     1261 ggtgactaat acgatttaat aataaaaaag gtgctaacta taaccactat atcatttata
     1321 gccttagtgt ataggtgtga agaaaaaata atatgcaata aaaaagggcc gctttcgcga
     1381 ccctttgttt ataaaaacct gacagaatta aaggttattt ttttccttca acccatttgc
     1441 catcaacata aaatgctgac cagccagtcg cctttccgtc tttttccgaa gagacatatt
     1501 gctgtttggt tttacggcta aagcgaacca tggtcttatt accttccgga tcctgctgtg
     1561 gcgcatcggc cagataacgc agtttttccg gcagacggtc gcggaagcga taaagctctt
     1621 ccaccagtgg cgcacgcgtt tcacgcgatt tcgggaaagt gttggcagcc aggaaaacac
     1681 ccgcagcgcc gtcacgcagc acgaaataag catctgattt ttcgcacggc agctcaggta
     1741 atggcaccgg atcttctttc ggtggtgcca cttcgccgtt acgtaaaatc ttacgtgtgt
     1801 ttttacactc ttcgttggtg caggccatgt atttaccgaa acgccccatt ttcaggtgca
     1861 tttcagagcc acatttttca cactcaacga tcgggccgtc ataaccttta atgcggaatt
     1921 cgccctcttc gatctcgtaa ccgtcgcagg ttgggttatt accacagaca tgcaacttac
     1981 gtttcggatc gatgagatag ctgtccatcg ccgtgccgca tttcgggcaa cgacgttttg
     2041 cgcgcagcgc gttggtttca gcgtcttcgc cttccagtac gttcagcact tcgttttccg
     2101 gcaccaggtt aatggtggtt ttgcaacgct ctttcggcgg cagcgcataa ccagaacagc
     2161 caaggaatac cccggtgctc gctgtgcgaa tccccatttt gcgaccacaa gtcgggcagt
     2221 caatgctggt cagaaccatc tggttcgggc gcataccacc ctcttccgga tctttttcag
     2281 ctttatctaa ctgctgggtg aaatccgaga agaagtgatc cagtacagct ttccactctg
     2341 cttcgtgatt tgccaccagg tcgaggctgt tttccatctg tgcggtgaag tcgtagttca
     2401 ttaactcgcg gaaattttct tcaaggcgat cggtgacgat ttcgccaatt ttttccgcat
     2461 agaaacgacg attttctact cgcacgtagc cacgatcctg aatggtcgaa atgatcgacg
     2521 cataggtaga cggacgaccg ataccgcgtt tttccagctc tttaaccagc gatgcttcac
     2581 tgaaacgggc tggcggcttg gtaaagtgct gggctggtgt aagttcaacg agcgtcagag
     2641 catcgccttt attaactgct ggtaagatgc gatcttcatc gcctttacgc aacgcaggca
     2701 tcacttttgt ccagccatca aaacgcaaaa tacgaccgcg tgctttcagg cggaaatcgc
     2761 ccgcaccaat ggtcagcgtc gtggagtcat atttcgctgg ggtcatctgg caggctacga
     2821 actgacgcca gattaactgg tacagtttct gcgcatctgc ttccatatcc ttcagcgatt
     2881 ccgccatcac attgacgtca gaaggacgaa tcgcttcgtg cgcttcctgt gagttttctt
     2941 tgctggcgta ctgattcgga ctttccggca gatatttctt accaaaatta tcgctgatat
     3001 aaccacgaac catatttacc gcgtcctgac tcaggttagt ggagtcggta cgcatgtaag
     3061 tgatatagcc tgcttcatac aaacgctgcg ccatcatcat ggtttttttc acgccaaatc
     3121 caagacgggt gctggcagct tgttgcagcg tagaggtaat aaaaggagcg ccaggtttac
     3181 tggttgtcgg tttgtcttca cgttccagca cgctgtagcg cgctttttcc agcagactta
     3241 ccgcagcctg agtttgttct ttgttgaccg gacggaacgg tttgtcgttc tgatgagtca
     3301 cctgtaacgc caacgcttca ccagatggcg tggtcgtgct ggcatcgact tcccagaact
     3361 cttccggcac gaacgcttta atttcacgct cacgctcgac caccaggcga accgccaccg
     3421 actgcacacg accggcagac agaccacgag cgatcttttt ccatagcagc ggcgaaacca
     3481 tataccccac cacgcggtcc ataaagcgac gcgcctgctg ggcattaaca cgatcaatat
     3541 tcagctcacc cggtttgtta aatgcctggc ggatcgcgtt tttagtaatt tcgttaaaca
     3601 ccactcggct atagcgcgca tcatcacccc caatcacttc ccgcaggtgc catgcaatgg
     3661 cttccccttc gcggtcaagg tcggttgcga gatagatgtg gtcggctttt tcagccagtt
     3721 gtttcagttc agagacgacc ttctctttac caggcaacac ttcatagtgc gcctcccaat
     3781 tgtgccacgg gtcaaccccc atacggttga cgagagcgcc acgttcatcc tttttaggct
     3841 ttttagccgt cttggtggag gtagagtcgg cactcttttt ggcagctgag ccactggtcg
     3901 gcaaatcgcg gatgtgaccg acgctggatt tcaccacgta gtcactaccc agatacttgt
     3961 tgatcgtttt ggcttttgcc ggggactcaa cgatgacaag agctttaccc atattcacct
     4021 tacctaattt gattcttcca ggaatgcgtc gcacgttgat aaaccttcca ctggcaacga
     4081 gttaccgata tggactcgtt ctctgcggat atcaacccct tgtcgtgact ctgttcacga
     4141 aaattcaggt gactgagtta acaggtcttg gcctacaggc gctatagcac gacggaattt
     4201 tggtcgaatg tcaagcaaat ctattgccag attgacgaaa gcgtcacact gtacctgata
     4261 aaatccgata cgcaacttta ttagcatgca aaatccaaag ttgccagtaa ggcgctcttg
     4321 ccaccagcgt ggtatatttg tctgcaagcc atgcctggcg tagactaatg tccttgttta
     4381 aggagtaaat aatgaacaaa gagacacaac ccatcgaccg cgaaacgctg ttgaaagagg
     4441 ccaacaaaat cattcgtgag catgaggata cgctcgcagg gattgaagcc accggcgtca
     4501 cgcagcgtaa tggcgtgctg gtatttatcg gcgactactt tttagatgaa caaggattgc
     4561 caacagccaa aagcaccgcg gtgtttaata tgttcaaaca cctggcccat gtgctttcag
     4621 aaaaatatca tctggttgat taaagcaaaa cgcgaggtct tagcctcgcg tttgtctttt
     4681 acatcaatgg cttttgaccc cgctgccacc agcgtagcaa caatcgatcg gcgctctctg
     4741 ccgcgctgcc ggtgaatcgg tcaatgagtc gtttacgctg catatagcgt acattaacca
     4801 cttcacggcc ttccatcagg ctaagaataa cttcatcact ggtgttgatt tcatcaacca
     4861 ggcctttctc taccgcctgt tgtccgtacc agtgttcacc cgttgccacc tgttcaatat
     4921 ccagagacgg acgcatacgc ttcacaaaat ctttaaataa ctgatgcgtt tcgttcagct
     4981 cttcgcggaa tttctcccgc ccttcttcgg tattttcacc cagcaaggtc agcgtgcgct
     5041 tatactgccc ggcggtgtgc agttcgatat caatatcttt gcttttcagg aagcggttaa
     5101 agttgggcat ttgcgccacc accccaatgg aacccacaat agcaaacggt gcggaaacaa
     5161 ttttgtccgc cacacaggcc atcatgtaac cgccgctggc agcgactttg tctaccgtaa
     5221 cagttaaagg aatgttttta tcacgcagac gctgcagctg cgaagccgcc aacccgtaac
     5281 catgcaccat gccaccaggg ctttccagac ggagcacaac ctgatcctgc ggtttgaatg
     5341 ctgcgagtac cgccgttatc tcttcacgta gcgagttcac ttcatgggcg tccatgctgc
     5401 ctttaaaatc cagcacccag acgcgaggtt tactgtcagt tgccacctcg cccagtttgg
     5461 ctttcgcttt tgctgctttc gcttcttgct tgtgcttttt cttctgcgct ttgtgccact
     5521 gtttttgctg atgtgagtcc atcagcgccg cggccagttc ttctttcatc tccttatact
     5581 gttcgctgag attgttgacc cgtaactcgc cacgctggcg tttattacgt tgagcaacat
     5641 tgacaataat ggcggcaatc gccgcaatcg ctagcacaac ggtaacgatt ttcgccaaaa
     5701 acaaaccata ttcagacaac aattccacga gtccccacct tggttaaaca acgctttggc
     5761 tcacagttta caacaggcat gttaagccgt ctcgcagaga tgttattgca gcgaaagtat
     5821 gacaaagtat ccattttgcg gtgttatcgc cccaggctga ttgaaatgcc cttctgtttc
     5881 aggcataaag ccccaaagtc ataaagtaca ctggcagcgc gacagacaac gtcgttgagg
     5941 agtagccatg cattaccagc caaaacaaga tttactcaat gatcgcatta tcctggtgac
     6001 gggagccagc gatgggattg gtcgtgaagc cgcgatgacg tatgcacgct atggtgcgac
     6061 agtgattctg ttgggccgta atgaagaaaa attacgtcag gtagccagcc acataaacga
     6121 agaaactggg cgtcagccac agtggtttat tctcgatttg ctgacctgca cgtccgaaaa
     6181 ttgccaacaa ctggcacagc gcattgtcgt taattatccg cgtctggatg gtgttttgca
     6241 taatgccgga ttgctcggcg atgtttgccc aatgagcgaa caaaatccgc aggtctggca
     6301 ggacgtcatg caggtcaacg ttaatgccac ctttatgctc acccaggcac tgcttccttt
     6361 attactcaaa tcggacgccg gttcactggt ctttacttca tcaagcgttg ggcgtcaggg
     6421 acgagccaac tggggtgcat atgcagcgtc gaaatttgcc accgaaggga tgatgcaggt
     6481 actggccgat gaatatcagc agcgcctgcg cgtcaactgc attaacccag gcggtacgcg
     6541 caccgcaatg cgcgcaagcg ccttcccgac cgaagatcca cagaaactta aaacacccgc
     6601 tgatatcatg ccgctctacc tctggctgat gggcgatgac agccgccgta aaaccggcat
     6661 gacctttgac gcccaaccgg gccgtaaacc aggaatttcc caatgagtga tgaacgctac
     6721 caacagcgtc agcagcgagt gaaagaaaaa gtagatgctc gtgtggccca ggcccaggat
     6781 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
     6841 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
     6901 ggcacctggc ctaatggcga acgcaatctg ctggagccac atggcgttga gtttcaggtg
     6961 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
     7021 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
     7081 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
     7141 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
     7201 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc
     7261 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
     7321 ggcatacttt gattaaccgt tattacgccc gccagaacga cgaccgccgc tcacctgact
     7381 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
     7441 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
     7501 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
     7561 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
     7621 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
     7681 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
     7741 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
     7801 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
     7861 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
     7921 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
     7981 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
     8041 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
     8101 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
     8161 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
     8221 tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg
     8281 actcactgat agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt
     8341 taactggcct ggctttgagg cgcaacatac tacactatct ttatggttaa agcttgtttc
     8401 gattaacatc agcttaatta ccagccacca ccgcctccgc cacccgcgcc gccaccagca
     8461 ccaccaccgg aataacttcg ccccaacaaa ctggttttgg cccccagact caggtgcatc
     8521 tgtaatgaaa acaaaacgcc actacgggca atttccgggg cgctaacagc cgctgtgagt
     8581 gatggggtga tacgtgctac ccattctctt ccaaggtttg ctgagactgc ccaacctagc
     8641 aacgcgtaat ccagtttccg tgtttcccct ttccgccgac gcccactgtg tgttgctgcc
     8701 tctttacgcg caagatagtt aacgatagct tcaccgtggg cataacgttg ctgcccagtt
     8761 tgtgtaaact ttggcagaaa aatatacccc atgccagtga gataaccggt caggaaaaca
     8821 gcgctaaaat aacccgctgg catataccag taaaagaata catctccgac attgatgtag
     8881 agagaataga aggccacgcc tgaacaaatc agtggtaaac aattcttccg tcctcaagga
     8941 tgcggccatt tcgatatgtg tcgccctctt ctccagtaaa gagatcgatt tcgctaaatg
     9001 gattacccgc gatatcaggg aacttgagtg aaaatcgggt cttatagatt tcaaacggcc
     9061 agtggttacc agtcacgttc cagattaaca gatctgaatc tccctcacgc aggaaagcat
     9121 ttttaacctg ataatgaatt tgataatgat agtcgccttg aggcaagaaa cgttgtttat
     9181 cgcccacgac aatactcatt aacccgatat gccagtcatg atgccagggt tcaggaatac
     9241 catcacgggt gacgccaaca atatgatagt tcagcagtgc agcgtcccca ccaggctgca
     9301 tccagagtcg tggtaaatca cgataaaatc cgtgtttaat ctgctgattt cgcgcctgaa
     9361 aatgaatatt ttctttgatg tcgagcaccc catccggttg cagagtggct tcaacatcaa
     9421 agagcgaaat cccttcttca taagaaggga tctccgtgtt ttctgcataa cttagagatg
     9481 atacaaatag ccctgctatc aacagcaaaa tgcagcgaaa cttccctgcc atagtcttcc
     9541 atccgtggaa cgcaatttgt tataagaaag gcttcacatc acctacgcct tcacgcacca
     9601 cgaccggcgt atcatcggta agatcaataa ccgttgtcgg tttctgcccg agatagccgc
     9661 cgtgaataat caaatctacc tgtttttcca gacggtcttt aatttcttcg ggatcggatt
     9721 cggtaaattc gctgcctggc agcattagcg aagtggaaag catcggttcg cccagtgctt
     9781 caagcaacgc ctgcgcaatc gggttagaag gcacacgcat accgatggtt ttgcgttttt
     9841 cctgcaacag acggcgtggc acttccttcg tccccttcag gatgaaggta tagttgcccg
     9901 gcgtgttgtt tttcattaaa cgaaacgcaa cgttatcaac aaatgaatag gtcgagagtt
     9961 cagaaagatc gcgacacatc agggtaaagt tgtgaccgtc cggcagctgg cgaatacgac
    10021 aaatacgctc catcgcgttt ttatcttcaa ttttacagcc gagcgcatag ccggaatcag
    10081 ttggataaac aatcaccccg cctttacgca cgatctccac cgcctggttg atcagacgtt
    10141 gctgtgggtt atcaggatga atataaaaaa actggctcat aattccctct ctgtggtgtt
    10201 ctgcggctgt tcccatagct gccagacgcc ttcaacgcct gcgggcagcc agagtttacg
    10261 acctagttca atccacgggc atggctgatg aaaatcagat ccttgtgatg cccataaatg
    10321 atgctgacgc gcaagggccg ccagctgggt acgttcattg ggcgattgct ggcactgcgc
    10381 gacttccatc gcgtcaccgt ggtgttcggc aaaatgcgca accaatcttt tcagccattt
    10441 agcactaaga ttgtaccgcc caggatgagc tagcaccgcc ttaccgccag aatgatgaat
    10501 gacatcaata gcttgttcta ttgtacacca ctgtggcgga acgtatccgg ttttcccgcg
    10561 cgccagatat tttttaaaga catccgccat tgaactggct ttgccgcacc ccacaaggaa
    10621 acgcgcaaaa tgaccgcgcg tcaccgcacc gccctgcgcc agtcgtcgcg cgccttccag
    10681 tgcccctggt atttgcgctt tttcaagtct ttcggcaatc agttgcgccc gctgattgcg
    10741 ccgttctgtc tgctgcgcga ggaactcaca cattagcgga tgagtaatat caatattcag
    10801 cccgacaata tgaatttcat gattttccca gaccgtggaa atttccacgc cgggaataag
    10861 attcagcgcc agtcccgaac gtgaaatttc ctctcttgct ggcacgattg cagctgtgtt
    10921 gtcatggtcg gtgatcgcca gggtaccgac gcgcatctcg actgcacggt gcaccaatac
    10981 ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt aaatcactgc
    11041 gtaattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc gccgacatca
    11101 taacagttct ggcaaatatt ctgaaatcag ctgttgacaa ttaatcatcg aactagttaa
    11161 ctagtgcgca agttcacgta aaaagggtat cgacaatgaa agcaattttc gtactgaaag
    11221 cttggtggcg cacttcctga aacgggcagt gtattcacca tgcgtaaagc aatcagatac
    11281 ccagcccgcc taatgagcgg gctttttttt gaacaaaatt agagaataac aatgcaaaca
    11341 caaaaaccga ctctcgaact gctaacctgc gaaggcgctt atcgcgacaa cccgactgcg
    11401 ctttttcacc agttgtgtgg gaatcgtccg gcaacgctgc tgctggaatc cgcagatatc
    11461 gacagcaaag atgatttaaa aagcctgcta ctggtagaca gtgcgctgcg cattacagca
    11521 ttaggtgaca ctgtcacaat tcaggcactt tccggcaacg gcgaagccct gctggcacta
    11581 ctggataacg ccctgcctgc gggtgtggaa aatgaacaat caccaaactg ccgtgtgctg
    11641 cgcttccccc ctgtcagtcc actgctggat gaagacgccc gcttatgctc cctttcggtt
    11701 tttgacgctt tccgtttatt gcagaatctg ttgaatgtac cgaaggaaga acgagaagca
    11761 atgttcttcg gcggcctgtt ctcttatgac cttgtggcgg gatttgaaga tttaccgcaa
    11821 ctgtcagcgg aaaataactg ccctgatttc tgtttttatc tcgctgaaac gctgatggtg
    11881 attgaccatc agaaaaaaaa cacccgcatt caggccagcc tgtttgctcc gaatgaagaa
    11941 gaaaaacaac gtctcactgc tcgcctgaac gatcttcgcc agcagctgac cgaagccgcg
    12001 ccgccgctgc cggtggtttc cgtgccgcat atgcgttgtg aatgtaatca gagcgatgaa
    12061 gagttcggtg gcgtagtgcg tttgttgcaa aaagcgattc gcgctggaga aattttccag
    12121 gtggtgccat ctcgccgttt ctctctgccc tgcccgtcac cgctggcggc ctattacgtg
    12181 ctgaaaaaga gtaatcccag cccgtacatg ttttttatgc aggataatga tttcaccctg
    12241 tttggcgcgt cgccggaaag ttcgctcaag tatgacgcca ccagccgcca gattgagatc
    12301 tacccgattg ccggaacacg tccacgcggt cgtcgtgccg atggctcgct ggacagagac
    12361 ctcgacaacc gcatcgaact ggaaatgcgt accgatcata aagagctttc tgaacatctg
    12421 atgctggtgg atctcgcccg taatgatctg gcacgcattt gcacccccgg cagcagctac
    12481 gtcgccgatc ttaccaaagt tgaccgttac tcttacgtga tgcacctggt ctcccgcgtg
    12541 gtcggtgagc tgcgccacga tctcgacgcc ctgcacgctt accgcgcctg tatgaatatg
    12601 gggacgttaa gcggtgcgcc gaaagtacgc gccatgcagt taattgccga ggcggaaggt
    12661 cgtcgccgcg gcagctacgg cggcgcggta ggttatttca ccgcgcatgg cgatctcgac
    12721 acctgcattg tgatccgctc ggcgctggtg gaaaacggta tcgccaccgt gcaagccggt
    12781 gctggcgtag tccttgattc tattccgcag tcggaagccg acgaaacccg taataaagcc
    12841 cgcgctgtac tgcgcgctat tgccaccgcg catcatgcac aggagacttt ctgatggctg
    12901 acattctgct gctcgataat atcgactctt ttacgtacaa cctggcagat cagttgcgca
    12961 gcaatgggca taatgtggtg atttaccgca accatattcc ggcgcagacc ttaattgaac
    13021 gcctggcgac gatgagcaat ccgatactga tgctttctcc tggccccggt gtgccgagcg
    13081 aagccggttg tatgccggaa ctcctcaccc gcttgcgtgg caagctgccc attattggca
    13141 tttgcctcgg acatcaggca attgtcgaag cttacggggg ctatgtcggt caggcgggcg
    13201 aaattcttca cggtaaagcg tcgagcattg aacatgacgg tcaggcgatg tttgccggat
    13261 taacaaaccc gctgccggtg gcgcgttatc actcgctggt tggcagtaac attccggccg
    13321 gtttaaccat caacgcccat tttaatggca tggtgatggc agtacgtcac gatgcggatc
    13381 gcgtttgtgg attccagttc catccggaat ccattctcac cacccagggc gctcgcctgc
    13441 tggaacaaac gctggcctgg gcgcagcaga aactagagcc agccaacacg ctgcaaccga
    13501 ttctggaaaa actgtatcag gcgcagacgc ttagccaaca agaaagccac cagctgtttt
    13561 cagcggtggt gcgtggcgag ctgaagccgg aacaactggc ggcggcgctg gtgagcatga
    13621 aaattcgcgg tgagcacccg aacgagatcg ccggggcagc aaccgcgcta ctggaaaacg
    13681 cagcgccgtt cccgcgccca gattatctgt ttgctgatat cgtcggtact ggcggtgacg
    13741 gcagcaacag tatcaatatt tctaccgcca gtgcgtttgt cgccgctgcc tgtgggctga
    13801 aagtggcgaa acacggcaac cgtagcgtct ccagtaaatc tggttcgtcc gatctgctgg
    13861 cggcgttcgg tattaatctt gatatgaacg ccgataaatc gcgccaggcg ctggatgagt
    13921 taggtgtttg tttcctcttt gcgccgaagt atcacaccgg attccgccac gcgatgccgg
    13981 ttcgccagca actgaaaacc cgcaccctgt tcaatgtgct ggggccattg attaacccgg
    14041 cgcatccgcc gctggcgtta attggtgttt atagtccgga actggtgctg ccgattgccg
    14101 aaaccttgcg cgtgctgggg tatcaacgcg cggcggtggt gcacagcggc gggatggatg
    14161 aagtttcatt acacgcgccg acaatcgttg ccgagctgca tgacggcgaa attaagagct
    14221 atcaattgac cgctgaagat tttggcctga ctccctacca ccaggagcaa ctggcaggcg
    14281 gaacaccgga agaaaaccgt gacattttaa cacgcttgtt acaaggtaaa ggcgacgccg
    14341 cccatgaagc agccgtcgct gcgaacgtcg ccatgttaat gcgcctgcat ggccatgaag
    14401 atctgcaagc caatgcgcaa accgttcttg aggtactgcg cagtggttcc gcttacgaca
    14461 gagttaccgc gctggcggca cgagggtaaa tgatgcaaac cgttttagcg aaaatcgtcg
    14521 cagacaaggc gatttgggta gaaacccgca aacagcagca accgctggcc agttttcaga
    14581 atgaggttca gccgagcacg cgacattttt atgatgcgct acagggtgca cgcacggcgt
    14641 ttattctgga gtgcaagaaa gcgtcgccgt caaaaggcgt gatccgtgat gatttcgatc
    14701 cggcacgcat tgcctccatt tataaacatt acgcttcggc aatttcggtg ctgactgatg
    14761 agaaatattt tcaggggagc tttgatttcc tccccatcgt cagccaaatc gccccgcagc
    14821 cgattttatg taaagacttc attatcgacc cttaccagat ctatctggcg cgctattacc
    14881 aggccgatgc ctgcttatta atgctttcag tactggatga cgaccaatat cgccagcttg
    14941 cagccgtcgc tcacagtctg gagatggggg tgctgaccga agtcagtaat gaagaggaac
    15001 tggagcgcgc cattgcattg ggggcaaagg tcgttggcat caacaaccgc gatctgcgtg
    15061 atttgtcgat tgatctcaac cgtacccgcg agcttgcgcc gaaactgggg cacaacgtga
    15121 cggtaatcag cgaatccggc atcaatactt acgctcaggt gcgcgagtta agccacttcg
    15181 ctaacggttt tctgattggt tcggcgttga tggcccatga cgatttgcac gccgccgtgc
    15241 gccgggtgtt gctgggtgag aataaagtat gtggtctgac gcgtgggcaa gatgctaaag
    15301 cagcttatga cgcgggcgcg atttacggtg ggttgatttt ggttgcgaca tcaccgcgtt
    15361 gcgtcaacgt tgaacaggcg caggaagtga tggctgcggc accgttgcag tatgttggcg
    15421 tgttccgcaa tcacgatatt gccgatgtgg tggacaaagc taaggtgtta tcgctggcgg
    15481 cagtgcaact gcatggtaat gaagatcagc tgtatatcga tacgctgcgt gaagctctgc
    15541 cagcacacgt tgccatctgg aaagcattaa gcgtcggtga aaccctgccc gcccgcgagc
    15601 ttcagcacgt tgataaatat gttttagaca acggccaggg tggaagcggg caacgtttcg
    15661 actggtcact attaaatggt caatcgcttg gcaacgttct gctggcgggg ggcttaggcg
    15721 cagataactg cgtggaagcg gcacaaaccg gctgcgccgg acttgatttt aattctgctg
    15781 tagagtcgca accgggcatc aaagacgcac gtcttttggc ctcggttttc cagacgctgc
    15841 gcgcatatta aggaaaggaa caatgacaac attacttaac ccctattttg gtgagtttgg
    15901 cggcatgtac gtgccacaaa tcctgatgcc tgctctgcgc cagctggaag aagcttttgt
    15961 cagcgcgcaa aaagatcctg aatttcaggc ccagttcaac gacctactga aaaactatgc
    16021 cgggcgtcca accgcactga ccaaatgcca gaacattacg gctggcacga acaccacgct
    16081 gtatctgaag cgcgaagatt tgctgcacgg cggcgcgcat aaaactaacc aggtgctcgg
    16141 tcaggcttta ctggcgaagc ggatgggtaa aaccgaaatc atcgccgaaa ccggtgccgg
    16201 tcagcatggc gtggcgtcgg cccttgccag cgccctgctc ggcctgaaat gccgtattta
    16261 tatgggtgcc aaagacgttg aacgccagtc gcctaacgtt tttcgtatgc gcttaatggg
    16321 tgcggaagtg atcccggtgc atagcggttc cgcgacgctg aaagatgcct gtaacgaggc
    16381 gctgcgcgac tggtccggta gttacgaaac cgcgcactat atgctgggca ccgcagctgg
    16441 cccgcatcct tatccgacca ttgtgcgtga gtttcagcgg atgattggcg aagaaaccaa
    16501 agcgcagatt ctggaaagag aaggtcgcct gccggatgcc gttatcgcct gtgttggcgg
    16561 cggttcgaat gccatcggca tgtttgctga tttcatcaat gaaaccaacg tcggcctgat
    16621 tggtgtggag ccaggtggtc acggtatcga aactggcgag cacggcgcac cgctaaaaca
    16681 tggtcgcgtg ggtatctatt tcggtatgaa agcgccgatg atgcaaaccg aagacgggca
    16741 gattgaagaa tcttactcca tctccgccgg attggatttc ccgtctgtcg gcccacaaca
    16801 cgcgtatctt aacagcactg gacgcgctga ttacgtgtct attaccgatg atgaagccct
    16861 tgaagccttc aagacgctgt gcctacacga agggatcatc ccggcgctgg aatcctccca
    16921 cgccctggcc catgcgctga aaatgatgcg cgaaaacccg gaaaaagagc agctactggt
    16981 ggttaacctt tccggtcgcg gcgataaaga catcttcacc gttcacgata ttttgaaagc
    17041 acgaggggaa atctgatgga acgctacgaa tctctgtttg cccagttgaa ggagcgcaaa
    17101 gaaggcgcat tcgttccttt cgtcacgctc ggggatccgg gcattgagca gtcattgaaa
    17161 attatcgata cgctaattga agccggtgct gacgcgctgg agttaggcat ccccttctcc
    17221 gacccactgg cagatggccc gacgattcaa aacgccactc tgcgcgcctt tgcggcaggt
    17281 gtgactccgg cacaatgttt tgaaatgctg gcactgattc gccagaaaca cccgaccatt
    17341 cccattggcc tgttgatgta tgccaatctg gtgtttaaca aaggcattga tgagttttat
    17401 gcccagtgcg agaaagtcgg cgtcgattcg gtgctggttg ccgatgtgcc aattgaagag
    17461 tccgcgccct tccgccaggc cgcgttgcgc cataatgtcg cacctatctt catctgcccg
    17521 ccaaatgccg atgacgacct gctgcgccag atagcctctt acggtcgtgg ttacacctat
    17581 ttgctgtcac gagcgggcgt gaccggcgca gaaaaccgcg ccgcgttacc cctcaatcat
    17641 ctggttgcga agctgaaaga gtacaacgct gcacctccat tgcaggggtt tggtatttcc
    17701 accccggatc aggtaaaagc agcgattgat gcaggagctg cgggcgcgat ttctggttca
    17761 gccattgtta aaatcatcga gcaacatatt aatgagccag agaaaatgct ggcggcactg
    17821 aaagtttttg tacaaccgat gaaagtggcg acgcgcagtt aatccccctg ccgccagttc
    17881 cgctggcggc attttaactt tctttaacga agccggaaaa atcctaaatt catttaatat
    17941 ttatcttttt accgtttcgc t
//
LOCUS       DAZSBF010000072        17876 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZSBF010000072 DAZSBF010000000
VERSION     DAZSBF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.073"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            149..827
                     /locus_tag="ACHXWD_001924"
                     /pseudo
     CDS             149..827
                     /locus_tag="ACHXWD_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            871..5721
                     /locus_tag="ACHXWD_001925"
     CDS             871..5721
                     /locus_tag="ACHXWD_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR7620854.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6089..7744
                     /gene="lldP"
                     /locus_tag="ACHXWD_001926"
     CDS             6089..7744
                     /gene="lldP"
                     /locus_tag="ACHXWD_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR7620855.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7744..8520
                     /gene="lldR"
                     /locus_tag="ACHXWD_001927"
     CDS             7744..8520
                     /gene="lldR"
                     /locus_tag="ACHXWD_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR7620856.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8517..9707
                     /gene="lldD"
                     /locus_tag="ACHXWD_001928"
     CDS             8517..9707
                     /gene="lldD"
                     /locus_tag="ACHXWD_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR7620857.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9903..10376
                     /gene="trmL"
                     /locus_tag="ACHXWD_001929"
     CDS             9903..10376
                     /gene="trmL"
                     /locus_tag="ACHXWD_001929"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR7620858.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10429..11250)
                     /gene="cysE"
                     /locus_tag="ACHXWD_001930"
     CDS             complement(10429..11250)
                     /gene="cysE"
                     /locus_tag="ACHXWD_001930"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR7620859.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11330..12349)
                     /gene="gpsA"
                     /locus_tag="ACHXWD_001931"
     CDS             complement(11330..12349)
                     /gene="gpsA"
                     /locus_tag="ACHXWD_001931"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR7620860.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12349..12816)
                     /gene="secB"
                     /locus_tag="ACHXWD_001932"
     CDS             complement(12349..12816)
                     /gene="secB"
                     /locus_tag="ACHXWD_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR7620861.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12879..13130)
                     /gene="grxC"
                     /locus_tag="ACHXWD_001933"
     CDS             complement(12879..13130)
                     /gene="grxC"
                     /locus_tag="ACHXWD_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR7620862.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13272..13703)
                     /locus_tag="ACHXWD_001934"
     CDS             complement(13272..13703)
                     /locus_tag="ACHXWD_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR7620863.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13948..15492
                     /gene="gpmM"
                     /locus_tag="ACHXWD_001935"
                     /gene_synonym="pgmI"
     CDS             13948..15492
                     /gene="gpmM"
                     /locus_tag="ACHXWD_001935"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR7620864.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15526..16785
                     /gene="envC"
                     /locus_tag="ACHXWD_001936"
     CDS             15526..16785
                     /gene="envC"
                     /locus_tag="ACHXWD_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR7620865.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16789..17748
                     /locus_tag="ACHXWD_001937"
     CDS             16789..17748
                     /locus_tag="ACHXWD_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR7620866.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17735..17876)
                     /locus_tag="ACHXWD_001938"
                     /pseudo
     CDS             complement(17735..17876)
                     /locus_tag="ACHXWD_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4484 a         4626 c         4675 g         4091 t
ORIGIN      
        1 gaatgataca tttttatgat gaatgcttaa aacaatattt aatcgacaca catcactgtc
       61 attaatgtaa agcaacatag atcaccaata atacaatgga caactctacc ggcattgatc
      121 gctggtcgga ggaaataagg atttttacat gaacttgaag aagatttttt tcagtgctgt
      181 cactgtttca gttctctgcg cattaacggg atgtgactac attgaagaag gcaagccaga
      241 aagtagttta ttgaaacaac aagaagaaca ttaaaattga tctacttgaa aaacaacaag
      301 cacagctaaa gagtcaactg gaaactattc aaaaacaaca aactggcata ataagcagta
      361 caaagacttt aactcacgta attaaatccg ttaaagatca acaaaacact tttattttta
      421 ccgagtttaa cccagcaaaa acgaagtatt tcatcctgaa caatggttcg gtggctttag
      481 cgggtcgagt gttatccatt gacgccacag agaatggcag tgttattcat atttcactgg
      541 tcaacttatt aagtacacct atctcaaata ttggttttaa tgcgacatgg ggtggtgaaa
      601 aacctgtcga tgccaaagag tttgcccggt ggcaacaatt acttttcaac acatcaatga
      661 aatccacatt gaaattatta ccaggtcaat ggcaagacat taatttgacc ctgaagggtg
      721 tatcgcccaa taacctggga tatctgaagt tagccatcaa catggaaaat attcagttcg
      781 acaatcttcc ctctgctgaa aatcggcaga aaagaagcaa aaaataaatg cagttagtac
      841 gcttataaca aggaatagcg agcatcatta atgaacaaaa tatttaaagt tatctggaac
      901 cctgcgacag ggaattatac tgttaccagc gaaacggcaa aaagccgtgg caagaaatct
      961 gggcgcagta agctgttaat ttctgcgctg gttgcgggtg gaatgttgtc gtcgtttggg
     1021 gcattggcga atgccgggaa tgacaacggc cagggtgttg attacggtag tggatcagct
     1081 ggcgacagct gggttgctat aggcaaaggg gcgaaagcaa atacttttat gaacaccagt
     1141 ggttccagta ctgctgtggg ttatgacgct atagctgaag gccaatatag ctctgccatc
     1201 gggtcaaaaa cccatgcgat tggtggtgca tcaatggcct ttggggttag tgcaatatca
     1261 gaaggcgata gaagtatagc actgggtgcc tcttcgtatt cattgggcca atactcaatg
     1321 gccctcggcc gttattcaaa agcattgggt aaattgtcta ttgctatggg ggactcttcc
     1381 aaagcggaag gagcaaacgc cattgccctg ggaaatgcca ctaaagctac tgagattatg
     1441 agtattgctc ttggcgacac cgccaatgcg tcaaaagcgt attcaatggc gctgggagca
     1501 agtagcgtcg catctgaaga aaacgcaatt gccctggggc gtagcagtgt agctagcggt
     1561 actgacagcc tcgcatttgg cagacaatca cttgccagcg cagcgaacgc tattgcgata
     1621 ggtgctgaga ccgaagccgc tgaaaatgca actgctattg gcaataatgc gaaggcaaaa
     1681 gggactaata gcatggcaat ggggttcgga agccttgccg ataaagtcaa tactatcgca
     1741 ttaggaaatg gcagccaggc tctggcagat aatgcaatcg ccataggcca gggcaacaaa
     1801 gctgatggcg tggatgccat cgctctgggt aatggtagcc agtcgagagg cttaaacacc
     1861 attgccttag gcacagccag taatgcaact ggtgataaga gtcttgcgct tggtagtaat
     1921 agcagtgcca acggtattaa ctctgtcgcg ctgggcgcag attccattgc ggatttagac
     1981 aataccgtct ctgtcggcaa tagttcatta aaacgcaaga tcgttaatgt gaaaaatggc
     2041 gcgatcaagt ctgacagtta cgatgccatt aatggttcac agctttatgc cattagcgac
     2101 tcggtagcaa aaaggcttgg aggaggggct gcagtagatg ttgatgacgg tactgttaca
     2161 gcaccaacct acaatttaaa aaatggtagc aaaaataacg taggggctgc gctcgctgta
     2221 cttgatgaaa acaccctgca atgggaccaa accaaaggca aatacagcgc tgctcatggt
     2281 actagtagcc caactgccag cgtaatcacc gatgttgcgg atggcacgat ttcagcctcc
     2341 agtaaggatg cggttaacgg ttcccaactg aaagctacca atgacgatgt cgaagccaac
     2401 accgccaata tcgctactaa taccagcaac attgccacga atacagcaag tattgccacc
     2461 aataccacca atatcaccaa cctgacggat tccgttggtg accttcaggc tgatgccctg
     2521 ctctggaacg aaactaaaaa ggcattcagt gcagctcacg gccaggatac caccagcaaa
     2581 atcaccaacg ttaaagatgc cgacctgacg gctgacagca ctgatgctgt taacggctct
     2641 cagctgaaaa ccaccaacga tgctgtggcg acgaatacca ccaatatcgc caataacact
     2701 tccaatattg ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt
     2761 ggtgaggatg cgctgaaatg ggataaggac aatggtgtat tcacggcagc tcatggcacc
     2821 gagaccacca gcaaaatcac caacgttaaa gatggcgacc tgacgactgg cagcaccgat
     2881 gccgttaacg gctctcagct gaaaaccacc aacgatgccg tggcgacgaa taccaccaat
     2941 atcgccacta acaccaccaa catctctaat ctgactgaga cggtgactaa tcttggtgag
     3001 gatgcgctga aatgggataa ggacaatggt gtcttcactg cagctcatgg caacaatacc
     3061 gccagcaaaa tcaccaatat cctggacggc acagtcactg caaccagttc cgatgccatt
     3121 aacggtagcc agctttatga cttaagcagc aatatcgcca cctacttcgg cggcaatgct
     3181 tctgtgaata ctgacggtgt gtttaccggt ccaacctaca aaatcggtga aacaaattat
     3241 tataacgtcg gcgatgcact ggctgcgatt aactcctcat ttagcacgtc tctcggcgat
     3301 gctctgcttt gggatgccac cgcaggtaaa ttcagtgcca aacacggtac taatggtgac
     3361 gcaagcgtga tcactgatgt cgcagatggt gaaatttcag actccagttc tgacgcagta
     3421 aacggctcac aactccacgg cgtgagcagt tatgttgttg atgcgctggg gggtggtgcc
     3481 gaagtcaatg cagacggcac catcactgcg ccgacgtaca ccattgctaa tgctgattac
     3541 gataatgtcg gtgatgccct gaatgctatc gataccactc ttgacgacgc tctgctctgg
     3601 gatgcggacg ccggtgaaaa tggtgcattt agcgccgctc acggaaaaga taaaactgcc
     3661 agtgtaatca ctaacgtcgc taacggtgca atctctgctg ccagcagcga cgcgattaac
     3721 ggctcacaac tctataccac caataagtac atcgctgatg cgctgggtgg tgacgcagaa
     3781 gtcaacgctg acggcaccat caccgcaccg acttacacca ttgcgaacgc cgagtacaac
     3841 aacgtcggtg acgccctgga tgcgcttgat gataacgccc tgctgtggga tgagactgcc
     3901 aatggcggtg ctggagccta caatgccagc catgacggta aagccagcat catcactaat
     3961 gtcgctaatg gcagtattag tgaggacagt accgatgcag tgaacggttc tcagttgaat
     4021 gcgacgaata tgatgattga gcagaacacc caaattatca atcagctcgc tggtaacacc
     4081 gacgcaacct atatcgaaga aaatggcgct ggtatcaact acgttcgtac taacgacaac
     4141 gatttagcct ttaacgatgc aagcgcctct ggtgtcggcg ctacagctgt aggttataac
     4201 gctgtcgcgt ctggtgccag cagcgtagcc attggtcaga acagcagcag caccgttgat
     4261 accggtattg cgctgggtag cagctccgtt tccagccgtg tgattgccaa aggttctcgt
     4321 gacactagcg taacggaaaa tggcgtggtg attggttatg acaccactga cggcgaactg
     4381 ctaggtgcat tgtcaattgg tgatgacggt aaataccgcc aaatcatcaa cgtagctgat
     4441 ggttcagaag cccatgacgc cgttacggtt cgccagttgc agaacgctat tggagcggtc
     4501 gccactacgc caaccaagta ctttcacgcc aactcaacgg cagaagactc actggccgtt
     4561 ggtgaagact cgctggcaat gggtgcgaaa actgtcgtta atggtaatgc aggtattggt
     4621 atcggtttga acactctggt tctagctgat gcgatcaacg gcattgctat cggcagtaac
     4681 gcacgcgcaa accatgcaaa cagtatcgca atgggtaacg gttctcagac cacccgtggt
     4741 gcacagactg gctacaccgc ctacaacatg gacgcaccgc agaactctgt aggtgagttt
     4801 tctgtcggta gtgaagacgg tcaacgtcag atcacaaacg tcgcagctgg ttcagcggat
     4861 accgatgcgg ttaacgtggg tcagttgaaa gtcactgatg agcgcgtagc gcaaaatacc
     4921 cagagcatta ctaacctgaa caatcaggtc actaatctgg atactcgcgt tactaatatc
     4981 gaaaacggta ttggcgacat tgtcaccacc ggtagcacca agtacttcaa gaccaacacc
     5041 gatggcgtag atgccaacgc ccagggtaaa gatagcgttg ctattggttc tggttccatt
     5101 gctgccgctg acaacagcgt cgcactgggt accggttccg ttgcagagga agaaaataca
     5161 atctctgtag gttcttctac taaccaacgc cgtatcacca acgtagctgc cggtgttaat
     5221 gccaccgatg cggttaacgt gtcgcagctg aaatcttctg aagcaggcgg cgtgcgctac
     5281 gacaccaaag ctgatggttc tatcgactat agcaatatca ccctcggtgg tggcaacggc
     5341 agtacgactc gtatcagcaa cgtctccgct ggcgtcaaca acaacgacgc ggtgaactac
     5401 gcgcagttga agcaaagcgt gcaggaaacg aagcaataca ccgatcagcg gatggttgag
     5461 atggataaca aactgtctaa aactgaaagc aagttgagcg gtggtatcgc ttctgcaatg
     5521 gcaatgaccg gtctgccgca ggcttataca ccgggtgcca gcatggcttc tattggtggc
     5581 ggtacttaca acggtgaatc ggcagttgct ttaggtgtat cgatggtgag cgccaatggt
     5641 cgttgggtct acaaattaca aggtagtacc aatagccagg gtgaatactc cgccgcactc
     5701 ggtgccggta ttcagtggta atgatccatt aagttagtgt gactaagggc aaacgccaaa
     5761 ttgcctgatg cgttacgcgc atcaggcaag ataaggcgcc ccgggtcaac aatctaaggg
     5821 caattctctg atgaggattg cccttttctt taccagacat ctccccccac aagaattggc
     5881 cctaccaatt cttcgcttat ctgacctctg gttcacaatt ccccaattaa aactcacatc
     5941 aatattgcca atacataaca tttagttaac cattcattgt cattatccct acacaacaca
     6001 attggcagtg ccacttttac acaacgtgtg acaaggagat gagcaacaga ctcattacac
     6061 gatgtgcgtg gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc
     6121 cgggaatatc tggctttcca gtctgatagc atcgcttccc atcctgtttt tcttctttgc
     6181 gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtag caatcgccct
     6241 tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta
     6301 tggcttcttc tatggtttgt ggcccatcgc gtggatcatt attgcagcag tgttcgtcta
     6361 taagatctcg gtgaaaaccg ggcagtttga catcattcgc tcgtctattc tttcgataac
     6421 ccctgaccag cgtctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg
     6481 agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg gcctgggttt
     6541 taaaccgctg tacgccgccg gactgtgcct gattgttaac accgcgccag tggcatttgg
     6601 cgcgatgggc attccaatcc tggttgccgg acaagtaaca ggcatcgaca gctttgagat
     6661 tggccagatg gtggggcggc agctaccgtt tatgaccatt atcgtgctgt tctggatcat
     6721 ggcgattatg gacggctggc gcggtatcaa ggagacgtgg cctgcggtcg tggttgcggg
     6781 cggctcgttt gccatcgctc agtaccttag ctctaacttc attgggccgg agctgccgga
     6841 cattatctct tcgctggtat cactgctctg cctgacgctg ttcctcaaac gctggcagcc
     6901 agtgcgcgta ttccgttttg gtgatttggg ggcgtcaaag attgatatga cgctggccca
     6961 caccggttac actgcgggtc aggtgttacg tgcctggaca ccgttcctgt tcctgacagc
     7021 taccgtaaca ctgtggagca tcccgccgtt taaagccctg ttcgcatcgg gtggtgcgct
     7081 gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat aaactggttg cccgtatgcc
     7141 accagtggtc agcgaggcta cagcctatgc cgccgtgttt aagtttgact ggttctctgc
     7201 caccggcacc gccattctgt ttgctgcact gctctcgatt gtctggctga agatgaaacc
     7261 gtctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctac ccatctactc
     7321 catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggactgt catcaacact
     7381 ggcactggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg
     7441 gctgggggta ttcctgaccg ggtcggatac ctcatctaac gccctgttcg ccgcgctgca
     7501 agccaccgca gcacaacaaa ttggcgtctc tgatctgttg ctggttgccg ccaataccac
     7561 cggtggcgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt
     7621 aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt
     7681 cacctgtatg gtgggcgtga tcaccacgct tcaggcttat gtcttaacgt ggatgattcc
     7741 ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg
     7801 attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgagcgcca actggcgatg
     7861 caactcggcg tatcacgtaa ttcactgcgc gaggcgctgg caaaactggt gagcgaaggc
     7921 gtgctgctca gtcgacgcgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg
     7981 gagcaaaaca tcgtccagcc gctaaaaacg ctgatggccg atgatccgga ttacagtttc
     8041 gatattctgg aagcccgcta cgccattgaa gccagcaccg catggcatgc ggcaatgcgc
     8101 gccacacctg gcgacaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac
     8161 ccggatatcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat
     8221 aacatcgtgc tgctgcaaac catgcgcggt ttcttcgatg tcctgcaatc ctcagtgaag
     8281 catagccgtc agcggatgta tctggtgcca ccggtttttt cacaactgac cgaacaacat
     8341 caggctgtca ttgacgccat ttttgctggt gatgccgacg gggcgcgtaa agcaatgatg
     8401 gcgcacctta gttttgttca caccaccatg aaacgattcg atgaagatca ggctcgccac
     8461 gcacggatta cccgcctgcc cggtgagcat aatgagcatt cgagggagaa aaacgcatga
     8521 ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt
     8581 tccactatat ggatggtggt gcatattctg aatacacgct gcgccgcaac gtggaagatt
     8641 tgtcagaagt ggcactgcgc cagcgtattc tgaaaaacat gtccgactta agcctggaaa
     8701 cgacgctgtt taatgagaaa ttgtcgatgc cggtagcact ggcaccggtg ggtttgtgtg
     8761 gcatgtatgc gcgtcgtggc gaagttcagg cagccaaagc ggcggacgcg catggcattc
     8821 cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtcgcgcca gccatcaagc
     8881 gcccaatgtg gttccagctt tatgtactgc gcgatcgcgg ctttatgcgt aacgcgctgg
     8941 agcgagcaaa agcagcgggt tgttcgacgc tggttttcac cgtggatatg ccgacaccgg
     9001 gcgcacgcta ccgtgatgcg cattcaggta tgagcggccc aaatgcggca atgcgccgct
     9061 acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccac
     9121 atgatttagg taatatctca gcttatctcg gcaaaccgac cggactggaa gattacatcg
     9181 gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg
     9241 atttctggga tggcccgatg gtgatcaaag ggatcctcga tccggaagat gcgcgcgatg
     9301 cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg
     9361 gtgtactctc ttccgctcgt gcgttgcccg ctattgcaga tgcggtgaaa ggtgatatcg
     9421 ccattctggc ggatagcgga attcgtaacg ggcttgatgt cgtgcgtatg attgcgctcg
     9481 gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc gctggcaaca gcgggccagg
     9541 cgggtgtagc taacctgcta aatctgatcg aaaaagagat gaaagtggcg atgacgctga
     9601 ctggcgcgaa atcgatcagt gaaattacgc aagattcgct ggtgcagggg ctgggtaaag
     9661 agttgcctac ggcactggct ccgatggcga aagggaatgc ggcatagtcg tttgcgcccc
     9721 ctcaccctaa ccctctccct cagggagagg ggaccgttcg gcgctgtatg tactccctca
     9781 ctctgaaacg acaccgcact ctttttttct ccctcgcccc tccggggagg gccggggtga
     9841 ggggaaaagg ccgcacggtt caccaatttt ctgctatcct gcccccgcac taagggggca
     9901 gtatgctaaa catcgtactt tacgaaccag aaattccgcc aaatactggc aacatcatcc
     9961 gtctttgcgc taataccggc tttcgtctgc atatcatcga accgatggga tttgcctggg
    10021 acgataagcg cctgcgccgc gcggggctgg actatcacga gtttaccgcc gttacgcgtc
    10081 atcatgacta tcgcgcgttc ctcgaagcag aaaatcccca gcgcctgttt gccctcacca
    10141 cgaaaggtac gcctgctcac agcgccgtaa gctatcagga tggcgattat ctgatgttcg
    10201 gcccggaaac acgcggcctg ccagcgagca ttcttgatgc cctgcccgct gaacaaaaaa
    10261 ttcgcattcc gatggtgccg gacagccgca gcatgaatct gtccaatgcg gtgtcggtag
    10321 tggtgtatga agcctggcgg cagctgggat atccgggagc gttgttgaga gattagttac
    10381 tgtatgccgg atagatgatt acatcgcatc cggcacgatc acaggacatt agatcccatc
    10441 cccatactca aatgtatggt taataccgtt gaaatgctgg tccatatcca ttgatggctt
    10501 atcgctgtct ggtttaccga caatacgagc cggaacgcca gcggcggttg tatgcggcgg
    10561 caccggttgc agcaccacgg aacctgcgcc aatcttcgcg ccgcgaccaa cttcaatatt
    10621 gccgaggatt ttcgcgcccg cgccaatcat cacaccttca cgaattttcg ggtgacggtc
    10681 accaccagat ttacccgtac cgccaagcgt cacagattgc agaatcgata cgtcgttttc
    10741 aattaccgcc gtttcaccca caacgatgcc cgtggcgtgg tcgagcataa tgccacggcc
    10801 aatttttgct gccgggtgaa tatcgacctg gaacgtcaca gaaacctggt tttgcagaaa
    10861 gattgccaat gcgcgacgcc cctgattcca caaccagtga ccgatgcgat aggcctgcaa
    10921 ggcatgaaaa cccttcaggt ataacaacgg ggttgagtat ttatcgactg ccgggtcgcg
    10981 ggtacgcacc gcctgaatat cacaggccgc agaggcgatc atttccgggt cagcggcgta
    11041 ggcttcttcc accacttcac ggatagcaat agcaggcata attggcgatg acagcttgtt
    11101 cgccagcatg tagctcagtg cactaccaag gttttcgtgc ttgagtagcg tcgcgtggta
    11161 aaaactggcc agcattggct cacagtccgc cagcgttctg gcttcggctt taatattgtt
    11221 ccagacaatt tccagttctt cacacgacat tgcttactcc acacgatgag ataatgaccg
    11281 gcccgttctg cgcgggccgg gtcatagcgg taacaaaggt tccctggggt tagtggctgc
    11341 tgcgctcgtc cttgcgtgca cgacctaata acgtcaatgc tgcctcgcgc gcgttttttc
    11401 cgcaatataa tacttgataa atttcctcgg ttattggcat ttctacgccg aagcgatgcg
    11461 ccagttcgcg gacttctttc gtattgcggt agccttccac cacctgacca atcttctcct
    11521 gcgcgctttg tacatccatg ccctgaccga gcatcatgcc aaaacggcgg ttacgcgact
    11581 ggttgtcggt acaggtaagc accagatcgc ccagccccgc catgcccata aaggtggcag
    11641 gatcggcacc cagcgccgcg ccaagacgcg acatttcagc cagcccacgg gtgatcagtg
    11701 ccgtacgcgc attcgcacca aaaccgatac cgtcggacat ccccgcgcca atggcaatga
    11761 cgtttttcac cgcaccgcca agctgcacac cgatgaaatc cgggttgctg taaacgcgga
    11821 aacttttgcc acagtgcagc aattgttgga gatcatcggc aaaggtctgg tcggtagagg
    11881 ccaacgaaat agccgtcggt aaacctgccg ccagttcttt cgcaaacgtt gggccagaga
    11941 taaccgccag cggaatttga tcgcctaagg cctcacgcgc cacgtcctgt aacagacgtc
    12001 cggtttccgc ttccagccct ttggtcgccc acaccagacg cgcatcagga cgcatcagcg
    12061 gtttaatctg gcgcagcact tcaccaaaga catggctggg tacgacgacg agaatattac
    12121 ggctggctgc cagcgcagtg gcgagatcgc tttcaagatg gagcgtatcg ggaaaaggca
    12181 catcggggag aaacgcggcg ttacagcggt cgcgttcaag cgttgcgata tgttcagggt
    12241 catggcccca gaggacaacc tcgtggccat ttcttgccag ggtaatggca agagcggtgc
    12301 cgtacgagcc ggcaccgatc acagtcattg aagcattacg ttggttcatc aggcatcctg
    12361 atgttcttca gtaccttcgc cagcctgctg ctgcaaatag ttcatgaaca gcgcatcgaa
    12421 gttaatcggc gcaaggttca gttgcgggaa tgtaccgcgg gataccacgc tggtgatgca
    12481 ctcacgagca tacgggaaca gaatgttcgg gcagtatgcg cccaggcaat gtgccatctg
    12541 ggtgccttcg atacccgcga tggagaaaat accgccctgc tgaacttcac acaggaacgc
    12601 ggtttcttcg cccagggagg ccgttacggt aacacgcagt accacttcgt atacgtcatc
    12661 tgccagttgg gaagatgccg tatccagatc aagtttaact tctggttgcc aatctttctg
    12721 gaaaacgtgc ggcgcgttcg gcgcttcgaa agagatatcc ttggtataaa tacgttggat
    12781 ctggaaagtc atttcagtgt tgttttgttc tgacatgtgt agaaaaccct taagtgttgt
    12841 ccttaaatac tgcgcaatgt aatgccaacg ttcacacgtt atttcagcag gggatccagt
    12901 ccaccacgtg catccaatgc atacaagtca tcacagccgc caatgtgctg tgcgtcaata
    12961 aaaatctggg gaaccgtggt gcgaccgctg cgtttgatca tctcttcacg cttggcggca
    13021 ttgccatcga tcggcaactc ctgaaaactc acgcccttgc tgctcagcag tgcttttgca
    13081 cgatggcaat acgggcaggt ttctttggta tagatttcaa cattggccat aatttagctc
    13141 ctgttttttt accctgaaat ttcatgttgc agggcggctg caaaatggag tgtccccaga
    13201 agcttataac aataagtcgc tgggggccta attgcagcta acggcctgca tcatgaaaga
    13261 cgacaggtaa attatttgcc gcgcaccaga ggcaagtttt cgccagccca gccagcaacg
    13321 ccctctttca gcacgaatac ttgcgcaaaa ccagctttcg tcagcgcgtt tgcaggctcc
    13381 tggcactgca tgccagaacc gtctaccacg ataaccggtt tgtctttgtg cttctcaagc
    13441 tcaccaacat tgttggcttt gatttcgctc ggcaacagat tgatagaacc tgcgatatgg
    13501 cctttacgga agtcatcacg ctgacgtaaa tccacaacca cagcgtcttc tttgttgatc
    13561 agacgcgtag cttcaccacg agtaatcacc ttcactttcg aggtcaggct cttaaacgta
    13621 gtcacaagaa ccgccaccag taacgcgatc caggcgatac tcagtatggg atgacggcca
    13681 acaaattgca taatttcttg catggggggt aacaactccc gacgtagtga ttaaaaaaac
    13741 caggaaagga gtatacctgc gcggtgcggc aaatacagcc agcgcgttaa ctggaatgca
    13801 attttgcggg gcacgacgaa aaaaaagcgc aaatctggca tcggcttgcc gcgagcggtc
    13861 gtatattttg atcttcagag gctattttat cgattcagct gtagtaaaat tacgcaaatt
    13921 ttgactcttg agtatgaggt tgtcgcaatg tcggtttcta aaaaacctat ggtactggtg
    13981 attctggatg gatatggcta tcgtgaagaa cagcaggata acgccatttt tagtgctaaa
    14041 accccggtaa tggatgcact gtgggccaat cgtccgcata ccctgatcga cgcttccggt
    14101 ctggaagtcg gtctgcctga ccgtcagatg ggtaactccg aagtaggcca cgttaacctg
    14161 ggtgccggcc gcatcgtgta tcaggacctg actcgtctgg acgttgaaat caaagatcgc
    14221 gctttctttg ctaatccagt gctgactggc gcggtagata aagcgaaaaa cgcaggtaaa
    14281 gcagtacaca ttatgggtct gctctccgca ggcggcgtac acagccacga agatcacatc
    14341 atggcgatgg tagaactggc agctgaacgc ggcgcagaaa aaatctacct gcacgcattc
    14401 cttgacggtc gcgacacccc gccgcgcagt gctgaatcct cgctgaaaaa atttgaagag
    14461 aaatttgctg cgctgggtaa aggccgcgta gcgtccatca ttggtcgtta ctacgcgatg
    14521 gaccgcgata accgttggga tcgcgttgaa aaagcctatg acctgctgac tctggcgcag
    14581 ggtgagtttc aggccgatac tgccgttgct ggtttgcagg cagcttatgc tcgcgacgaa
    14641 aacgacgagt tcgtgaaagc gaccgttatt cgtgcagaag gtcagccaga tgcggcaatg
    14701 gaagacggcg atgcgctgat tttcatgaac ttccgtgctg accgcgcgcg tgaaatcact
    14761 cgtgcattcg tgaacgctga tttcgatggc ttcgcgcgta agaaagtggt taacgtcgat
    14821 ttcgtgatgc tgaccgaata cgccgctgac atcaaaactg cggttgctta cccacccgct
    14881 tccctggtta acaccttcgg cgagtggatg gcgaaaaacg acaaaactca gttgcgtatt
    14941 tccgaaaccg aaaaatatgc ccacgttact ttcttcttca acggtggcgt agaagagtcg
    15001 ttcaaaggcg aagatcgcat tctgatcaac tcaccgaaag tggctaccta cgatctgcaa
    15061 ccggaaatga gctccgcaga gctgaccgaa aaactggttg cggccatcaa gagcggcaaa
    15121 tacgacacca ttatctgtaa ctatccgaac ggcgacatgg taggtcacac cggggtgatg
    15181 gaagcggcgg ttaaagcggt tgaagcactg gatcactgcg tggaagaagt cgcgaaagcg
    15241 gttgagtccg tgggtggaca actgctgatc accgctgacc acggtaacgc tgaacagatg
    15301 cgcgatccgg caacgggtca ggcacacacg gcacacacca acctgccagt tccgctgatt
    15361 tacgttggtg ataagaacgt gaaagcggtt gcaggcggca aactttctga catcgcgccg
    15421 accatgttgt cgctgatggg tatggaaatc ccgcaagaga tgactggtaa gccgctgttc
    15481 atcgtggaat aatccctccc catgagggga aaggcgatta ataccatgac acgggccgtg
    15541 aaaccgcgca ggtttgcaat caggcccatc atctacgcca gcgttcttag cgctggcgta
    15601 ttgttgtgcg ccttttccgc ccacgcggat gagcgtgacc aactcaaatc tattcaggcc
    15661 gatatcgcca caaaagagcg cgcggtacgc caaaagcaac aacaacgcgc aagcctgctc
    15721 gcacaattga aaaagcagga agaagcgatc tctgaagcca cccgtaagct gcgcgaaacg
    15781 caaaacacgc tcaatcaact caataaacag attgatgaga tgaacgcgtc gattgccaaa
    15841 ctggagcagc aaaaagccgc ccaggagcgc agcctcgccg cacaactgga tgccgcattc
    15901 cgtcagggcg agcataccgg tattcagcta attctcagcg gtgaagaaag ccagcgtgga
    15961 cagcgtttac aggcttattt cggctatctc aaccaggcgc gacaagaaac cattgcccag
    16021 ttgaagcaaa cgcgtgaaga agtcgccatg cagcgtgctg aactggaaga gaaacaaagc
    16081 gagcaacaaa cgctgttata tgaacagcgc gcccaacagg cgaaactgac tcaggctctg
    16141 aacgagcgta aaaagacgct ggcggggctg gaatcttcca tccagcaagg tcagcagcag
    16201 ctaagtgaac tgcgtgccaa cgaatcccgt ctgcgtaaca gtattgcccg tgcggaagcc
    16261 gcggcgaaag cgcgtgctga acgagaagcg cgtgaggctc aggcagttcg cgacaggcag
    16321 aaagaagcga cgcgcaaagg caccacctac aaaccgaccg aaagcgaaaa atcgctgatg
    16381 tcccgaactg gtggcctggg tgcgccgcgt ggtcaggcat tctggccagt tcgtggccct
    16441 acgctgcatc gctatggcga gcagctacag ggtgaattac gctggaaagg aatggttatc
    16501 ggtgcctctg aaggtactga agttaaagca attgccgacg gtcgggtgat tctggctgac
    16561 tggctgcaag gctacggtct ggtggtggtg gttgagcacg gtaaaggcga tatgagtctt
    16621 tacggctata atcaaagcgc gctggtgagc gttggttcgc aggttcgtgc gggccagcca
    16681 attgcactgg tgggcagcag tggcggtcag ggcagacctt cactctattt cgaaattcgc
    16741 cgccagggtc aggccgtcaa tccacagccg tggttgggaa gataagtttt gtttccattt
    16801 cgtcgtaacg ttcttgcatt tgccgctctg ttggcgctct cctcccctgt aattgctggc
    16861 aaacttgcca tcgtcattga tgattttggg tatcgcccga acaacgaaaa ccaggtgctg
    16921 gcgatgcctt ccgctatctc cgtcgctgta ttacccgatt caccgcacgc cagagagatg
    16981 gcgaccaaag cgcataacag cggtcacgaa gtgttgattc atctcccgat ggcaccgttg
    17041 agtaaacagc cgctggagaa aaatacgctg cgcccggaga tgagcagcga cgaaattgag
    17101 cgcattattc gtagtgcggt caataacgtg ccctatgccg tggggatcaa taaccacatg
    17161 ggcagcaaga tgacctctaa cctgtttggt atgcagaaag tgatgcaggc gctggagcgt
    17221 tataatcttt acttcctcga cagcgtaacc attggtaata cccaggcgat gcgcgccgcg
    17281 caaggcactg gcgtgaaggt gatcaaacgg aaggtgttcc tcgacgattc acagaacgaa
    17341 gcggacatcc gtgtgcaatt caatcgcgca attgacctag cgcgtcgcaa cggatcaacc
    17401 attgccattg ggcatcctca cccttcaacg gtacgcgtgt tgcaacagat ggtttataac
    17461 ctgccgccag acattacgct ggtgaaagcc agcagcttgc tgaatgaacc gcaggttgat
    17521 acgtctacac cgccgaaaaa cactgtgccc gacacaccgc gtaatccgtt ccgtggcgtg
    17581 aagctgtgca aaccgaagaa accgatagaa cctgtttatg ctaatcgctt ctttgaagta
    17641 ttaagcgaaa gcatcagcca gagcacgctg atcgtttact tccagcatca gtggcaaggc
    17701 tggggcaaac agcctgaagc ggcgaagctt aacgctagcg taaattaaag gcgctacgcg
    17761 taatgcgatg gtgcgaccgc gttttgtcgc gccattgcca taagcggaaa gactacagta
    17821 acgcctgata acccaccttt acgctgcgca cattggtaat caggcgctta tacata
//
LOCUS       DAZSBF010000073        17684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZSBF010000073 DAZSBF010000000
VERSION     DAZSBF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.074"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(77..1483)
                     /gene="gndA"
                     /locus_tag="ACHXWD_001939"
     CDS             complement(77..1483)
                     /gene="gndA"
                     /locus_tag="ACHXWD_001939"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR7620867.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(1647..3017)
                     /gene="cpsG"
                     /locus_tag="ACHXWD_001940"
                     /gene_synonym="manB"
     CDS             complement(1647..3017)
                     /gene="cpsG"
                     /locus_tag="ACHXWD_001940"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR7620868.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(3122..4558)
                     /gene="cpsB"
                     /locus_tag="ACHXWD_001941"
     CDS             complement(3122..4558)
                     /gene="cpsB"
                     /locus_tag="ACHXWD_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR7620869.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(4561..5784)
                     /gene="wcaI"
                     /locus_tag="ACHXWD_001942"
     CDS             complement(4561..5784)
                     /gene="wcaI"
                     /locus_tag="ACHXWD_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR7620870.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(5781..6260)
                     /gene="gmm"
                     /locus_tag="ACHXWD_001943"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(5781..6260)
                     /gene="gmm"
                     /locus_tag="ACHXWD_001943"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR7620871.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(6263..7228)
                     /gene="fcl"
                     /locus_tag="ACHXWD_001944"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(6263..7228)
                     /gene="fcl"
                     /locus_tag="ACHXWD_001944"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR7620872.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(7231..8352)
                     /gene="gmd"
                     /locus_tag="ACHXWD_001945"
     CDS             complement(7231..8352)
                     /gene="gmd"
                     /locus_tag="ACHXWD_001945"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR7620873.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(8378..8926)
                     /gene="wcaF"
                     /locus_tag="ACHXWD_001946"
     CDS             complement(8378..8926)
                     /gene="wcaF"
                     /locus_tag="ACHXWD_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR7620874.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(8942..9688)
                     /gene="wcaE"
                     /locus_tag="ACHXWD_001947"
     CDS             complement(8942..9688)
                     /gene="wcaE"
                     /locus_tag="ACHXWD_001947"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR7620875.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(9699..10916)
                     /gene="wcaD"
                     /locus_tag="ACHXWD_001948"
     CDS             complement(9699..10916)
                     /gene="wcaD"
                     /locus_tag="ACHXWD_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR7620876.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(10891..12108)
                     /gene="wcaC"
                     /locus_tag="ACHXWD_001949"
     CDS             complement(10891..12108)
                     /gene="wcaC"
                     /locus_tag="ACHXWD_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR7620877.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(12105..12593)
                     /gene="wcaB"
                     /locus_tag="ACHXWD_001950"
     CDS             complement(12105..12593)
                     /gene="wcaB"
                     /locus_tag="ACHXWD_001950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR7620878.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(12596..13435)
                     /gene="wcaA"
                     /locus_tag="ACHXWD_001951"
     CDS             complement(12596..13435)
                     /gene="wcaA"
                     /locus_tag="ACHXWD_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR7620879.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(13528..15690)
                     /gene="wzc"
                     /locus_tag="ACHXWD_001952"
     CDS             complement(13528..15690)
                     /gene="wzc"
                     /locus_tag="ACHXWD_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR7620880.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(15693..16136)
                     /gene="wzb"
                     /locus_tag="ACHXWD_001953"
     CDS             complement(15693..16136)
                     /gene="wzb"
                     /locus_tag="ACHXWD_001953"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR7620881.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(16142..17281)
                     /gene="wza"
                     /locus_tag="ACHXWD_001954"
     CDS             complement(16142..17281)
                     /gene="wza"
                     /locus_tag="ACHXWD_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR7620882.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         4094 a         4773 c         4421 g         4396 t
ORIGIN      
        1 tctataagat aaatggcaaa aaaaaagccc ggtgcaatat acgccgggcc tcaattttac
       61 tgttggttaa atcagattaa tccagccatt cagtatggaa cacaccttct ttatcaatgc
      121 gcttataagt atgtgcaccg aaatagtcac gctgtgcctg gatcaggttc gcaggcagaa
      181 cagcggcacg gtagctatcg taataggcaa ccgcagcggc gaaggtcgga accgggatac
      241 cgttctgtac tgcataagca acgacatcac gcagcgcctg ctggtagtca tcggcaattt
      301 gcttgaagta cggagccagc agcaggttag cgatctgcgg attttcggca tatgcatcgg
      361 tgattttctg caggaactgc gcacggatga tgcagccagc acggaaaatc ttcgcgattt
      421 cgccgtagtt cagatcccag ttgtattctt cagacgcagc acgcagctga gagaagccct
      481 gagcgtaaga aacgattttg cccagataca gcgcacggcg aactttttcg atgaactcag
      541 ccttgtcgcc tgctgactga gcttgcggac cagagagaac tttagatgcg gcaacgcgct
      601 gatctttcag agaagagata taacgtgcaa acacagactc ggtaatcagc gacagcggtt
      661 ctccgagatc cagcgcgctc tggctggtcc atttaccggt acctttgtta gccgcttcat
      721 ccaggatcac atcaaccagg tagttaccgt cttcatcttt tttagtgaag atatctttgg
      781 tgatgtcgat caggtagctg ctcagttcac cgttattcca ctcggtaaag gtctgtgcca
      841 gttcttcgtt agagagattc aggccgcctt tcaggagaga ataggcttca gcaatcagtt
      901 gcatatcacc gtattcaata ccgttgtgaa ccatcttcac ataatggcct gcgccatcag
      961 caccgatata ggtaacgcac ggttcgccat cttcagcaac ggcggcgatt ttggtcagga
     1021 tcggcgcaac cagttcatag gcttctttct ggccaccagg catgatggac ggccctttca
     1081 gcgccccctc ttctccgccg gaaacaccgg taccgatgaa gttaaagcct tcggcagaaa
     1141 gctcacggtt acgacgaatg gtgtcctgga agaaggtgtt accaccatcg atgatgatgt
     1201 cgcctttatc gagatatggt ttgagcgaat caatagcagc atccgtgcct gcacctgctt
     1261 tcaccattaa caggatgcga cgaggcgttt ccagagattc aacaaactct ttcaccgtat
     1321 agtaaggaac cagtttcttg cctggatttt cggcaatcac ttcttccgtc ttttcacggg
     1381 aacggttgaa aatagagacg gtataaccac ggctttcgat gttgagcgca aggttgcgcc
     1441 ccatcactgc cataccgact acgccgatct gttgctttga cattgtttac tcctgtcagg
     1501 gggtggtcac cgccgagtgt gcggcttaaa tgtggtatag atgttaactc actcatagag
     1561 atggtgaata gcattacctt tgctatacca cactgaatat ctcacctttt ccgtctttta
     1621 aaacttattt atatgaaatt actgaattac ttgttcagta actcaagaat aagttttgtc
     1681 ttttcttcca tcagcggcac atcaccgcga gattccacat tcaaccgcac caccggttcg
     1741 gtgttggagg tgcgcaggtt aaagcgccag tcggcaaagg tcatgctgat gccatcggtg
     1801 cgatccaccg ccagcgcctc gcggctaaaa tgctgttcca cgcggttaat cgcctcaaag
     1861 ggttgcgcca gtttgctgtt gatctcaccg cttgccggaa acgctgccat ccggtcgcgc
     1921 accagttcgc ccagcgtttt tcctttcagg cacaccagtt cggcgaccag cagccacggg
     1981 atcatgccgc tgtcgcagta agcgaaatca cggaagtaat ggtgggcgct catttcacca
     2041 ccgtagatgg cgtcttcctt gcgcatccgt tctttaataa aggcgtgtcc ggttttcgac
     2101 atcaccggcg tgccgcctgc ggcagtcacc acatcaacgg tgttccagga gagacgcgga
     2161 tcgtggatga tcttcgcgcc gggatttttt tcgaggaatg cttctgccag caggccgaca
     2221 atgtagtagc cctcaataaa ctgccctttt tcgtcaaaca ggaagcagcg gtcaaaatcg
     2281 ccatcaaaag caatgcccat atccgcaccg tgtttgatga ccgcattgcg ggtgtcgtcg
     2341 cggcattccg gcagtagcgg gttaggaata ccgttgggga aattgccgtc cggcgtgttg
     2401 tgcactttga ttaattccac gggtgcgcca agggctttaa agcgggcttc gatggcgtcc
     2461 accaccggac ccgctgcgcc attcccggag ttaatcacca gcttgagcgg cgtgaggttt
     2521 ttgacattga tataaccgaa caggtgatca acgtaagcgt cacgcagatt gatttgctga
     2581 tagcgaccgc gtttggtttc atcaacggga ggaaagtcgt tggcttctgc cagacgctgg
     2641 acatcgcgca gtccggtatc accgctgatc gggcgagccc cttcgcgcac cagtttcatg
     2701 ccgttgtaat ccatcgggtt atggctggcg gtaacttcaa tgccgccatc cacgccgaga
     2761 tggaacgtgg cgaaatagat ctcttcggtg ccggacatgc caatatccag cacatcgacg
     2821 cccgcatcct gtaaacctct cgccagcgcc agttttaagg tttcgctggt gaggcggaca
     2881 tcaccgccta acacaatggt tttcggtttg agaaattcgc cataggcgcg accaatgcgc
     2941 caggcgatat cttcattcag ttcttcgcct aatttcccgc gaatatcata ggctttaaag
     3001 caggttaatt ttttcatgtc gttacccttt ttcaggcaat agttggccct gaccgaaacg
     3061 gccaattttg tttttgtcgt tatttacatc tcggtttcgc attcattgcc tgatgcgacg
     3121 tttacacccg tccgtagcga tccgcgaaac gcaccacatc atcctcttcg agataagagc
     3181 cggagcgcac ttcaattaaa tcgagcggaa ttttccccgg gttttccagg caatgcatcg
     3241 cccccagcgg aatataaatg gactcgtttt caccaagcag tttgatatca ccatcaatgg
     3301 tgacttttgc cgttcccgcg acaaccaccc agtgttccgc gcggtgatgg tgcatctgta
     3361 ccgacaagcc ctcgcccggt ttcacggtga tgcgtttcac ctggtagcgg tcgcccgcgt
     3421 cgatagagtc atatttgccc cacgggcgat agacttcgcg atgcacccga tgctcatggc
     3481 gaccatcggc tttgatctgc tcgaccactt ttttcacatc ctgcaccgcg ttacggtcgg
     3541 caatcagcac cgcatctttg gtctgcacca ctaccagatc tttcaccccg acggtggtta
     3601 cgaggccaga ttcggcgtac acatagctgt tttcagtttt gtgattaatc acatcgccgt
     3661 ggcaaacgtt gccctcggcg gtgtgggcgc taatttccca taatgaggac caggagccga
     3721 catcgctcca gcccgcatcc atcggcacca caacggcatc tgccgtacgt tccatgaccg
     3781 cgtaatccac cgactcttcc gggcaggcga gaaacgcttc ttcatccaca cgaataaaat
     3841 cgagatccgg atcgacggcg ctcatcgctt tttcacaggc atcgagaata tccggacgat
     3901 attttttcag ttcttcgaga tagcgtccgg cgcggaacag gaacatgccg ctgttccagt
     3961 aatattcgcc gcttgccaca taggcctggg cggtttccag attcggtttt tcgacaaact
     4021 gcgccacttc aaaggccacc gtatcctgct cacccgcaga cacttcaccg cgacgaatat
     4081 agccataacc ggtttctggt agatccggca caatgccgaa ggtcaccagc ttgcccgctt
     4141 cggcatatgg catggcatta cgcacggcgg cacggaacgc gtcttcatcg gcaatcacat
     4201 gatccgccgc caataccagc attaacgggt cgctctccgg gctatgacgt tttgccgcca
     4261 gcgccgccag cgcaatggca ggtgccgtgt ttcgccctgc cggttcgaga ataatgttct
     4321 cggtaagttt gttcagttga cgcagctgtt ccgcgacaat aaagcggtgc tgctcattgc
     4381 aaatcaccac cgggctttcg cactccacgc cgttcaggcg gcagatggtg gtttgcagca
     4441 tggtgagatc gcctttcagg cataaaaact gcttggggta aagtacgcgg gaaagcggcc
     4501 ataagcggct accggagcca cctgccatca caactggata gagtttcgac tgcgccataa
     4561 ttatccccga atatcattta taaattgacg taacacgttc tctttatcga gtgtgcgttc
     4621 ggcatattca cgtgccaccg tgttgtgttt gggcagcagg agcgcctgac ggatccccgc
     4681 caccagcgcc tcgaccgatt ccggttctac gcaaaccgca atgcccggaa aggtttcgca
     4741 aagctgcccc agttctgtgt gggcctctgc ggtaatcacc gcgttaccgc ctaccgccag
     4801 aatattggtc agtttcgacg gcaatacggc atctgccgcg ccgcgttttt gcaccaccag
     4861 atggcaatcg cccatcttca gcagtgcggg taaagcgtca tacgattgca gcgggaaaaa
     4921 ttgcatgttg cgcagtccac gctgctgcgc cattttttcc agccgcgctt tgccgccgcc
     4981 ctgcccgaca atggcaaaaa tcagcggttc atcgcgcaga cgatcggcag cttcaataac
     5041 gttttccagc ccctgctttt caccaatatt gccggagtaa agaatgattt ttttgttatc
     5101 cggcaggtca agctggttac gaagggcgtc aacatcggca tctgcaacat gctgaaaacg
     5161 ggcaatttcc gaccagttgg ggaagaagat gacgttttcc gccgccacgc ctttttcgat
     5221 ggctttattc atcatcgaac gcgaaatcgt ggagacgtta tcgacgttat gcagtccgct
     5281 acgttcgaac gccgttgcca gctgtgccac tttgctgcct ttgccttttc cggcaaggcc
     5341 cagccccagc atggcgtcca cttcgtaatc ctgaatatgc agcacggtac gcgcaccaga
     5401 aagtttcgcc agcaggcgca ttcccggcgt gcaaaacagc gttggcacca cgccgataat
     5461 gcgatccggc ttccagcgac gttgcgccat cagaggaaag aaactgctga cggcaaaact
     5521 gccgagatgt aacaggcgtt tcagggtgct cggctgtttt ggcacgtaca gcgggcagcg
     5581 ccacaccgtg gcggccccct cttctcgttt gtaccgccag gcggaatagt tctcgcccac
     5641 ctgccactgc gggtagtaag gcggtgcggt aataacccgc acctcatgac cttgtgccgc
     5701 cagccattcc accatctcgc cggtgtattt gccgataccg gttaactccg gcgagtagtt
     5761 aatgccgtag accagtattt tcataatccg ggtactccgg cacgcttctc agcgagaaaa
     5821 taggcgcggc tgttagcgtg aacatcgtta ctggcgagca gcgcgtccgg cgtcagccag
     5881 cggtaatcgt catgctgctc atccggcagt atcagctctt cttcagcgac tctgaagcga
     5941 aaaccgagca ccacatagtg agtggtgaaa tccgtgccgg agaagttatc gtcataaaag
     6001 tgctgccaga caccgtaaaa ctggcctgct gttatcggca gacgcagccc cagttccgcc
     6061 atcgtcagcc gctcgaatgc ggcttccagc gtttcgtctt tctgcacgcg ccctcccggc
     6121 acaaaccagt aaccctgcgc cgggcggttg gttcttttgc caagcagaaa ctcaccgcga
     6181 ctgttctcga caataaagtc gagagagaca agcggggtgg agcgcactac cgtggcaaag
     6241 tcttcctgac gtaaaaacat cattaccccc gaaagcggtc ttgattctca aggaaccact
     6301 ggtaagtgct ggcaagcccc gcttccagtg agatttcgtg ataccagcca agctgatgca
     6361 ggcgcgtcac atccagcagt ttgcgcggcg taccatccgg tttgctggca tcaaaaacca
     6421 cccgaccttt gtaacccacc actttggcga tggtttgcgc cagctcgcgg atagtgcagt
     6481 caacgcccgt gccgacgtta atgtgcgaca gcatcggctg ggtgttctcc agccagactt
     6541 cgtgcgccag ctccatgaca tgaatgctcg ccgccgccat atcatcgacg tgcaggaatt
     6601 cacgcatcgg tgtaccgctg ccccatacca ccacgtccgg tgcattctgt gccgtcgcct
     6661 cgtggaagcg acgcagcaat gcagggatca catgcgaatt actcgggtgg aagttgtcat
     6721 gcgggccata caggttggtc ggcatgactg agcggtaatc gcgtccgtac tggcggttgt
     6781 atgattcgca cagtttgatc ccggcgattt tggcaatggc gtaaggctcg ttggtcggct
     6841 ccagcgtgcc ctgcaataac tcgctttctg ccatcggctg ttttgccagt ttcgggtaga
     6901 tacaggacga tccaagaaac agcagtttgt tcacgtcgtt ctgatgcgcg gcgtgaatga
     6961 tgttgctctc aatcatcatg ttctggtaga tgaaatccgc cggataggtg ttgttggcaa
     7021 caatgccgcc cactttcgcc gccgccagat agacctggtc aatgcgttcg ctggcaaaga
     7081 aatcatgcac cgcgcggctg tccagcaggt tcagctcgtc gcgggtgcgt aataccagtt
     7141 ccacatcacc gcgctgttcg agctgccgcc tgatggcaga accgaccatc ccacgatggc
     7201 cagcgataaa aatgcgttgt ttactcatgc ttatgactcc agcgcgatcg ccacgtcgta
     7261 gccgtgagat ttcagcagag agtgtttttt cgccgcttcg aggtcattag ccaccatttc
     7321 agacaccatc tctctgaggg tgatttccgg tttccagccc agtttttcgt gcgctttggt
     7381 cgggtcgccg agcagcgttt caacttcagc cggacggaag taacgcggat caacggcgat
     7441 aatcacatca cccggtttaa cgcccggcgc gtcatgcccg gtgacggaaa ccacaatgcc
     7501 cttctcttca acacccgtgc cttcaaagcg cagtttgatg cccagctgtg ccgctgccat
     7561 ttccacgaac tgacgcacgg agtactgaac gccggtcgcg ataacgaaat cttccggctg
     7621 ttcctgttgc agcatcatcc actgcatttt tacgtagtct ttggcgtggc cccagtcacg
     7681 cagggaatcc atattgccga ggtacaggca cgactccagc ccctgggcga tgttggcgat
     7741 tgcgcgggtg attttgcggg taacgaaggt ttcgccgcgg cgcggggatt catggttgaa
     7801 gagaattccg ttacaagcgt acatgccgta ggattcgcgg tagttaacgg tgatccagta
     7861 ggcgtacagt ttggcgaccg catacggaga tcgcgggtag aacggcgtgg tttctttctg
     7921 cggaatttcc tgcaccagac catacagttc agaggtggaa gcctgataga aacgagtttt
     7981 cttttccaga ccgaggaagc ggatcgcctc cagcaggcgc agcgtaccca tcgcgtcgac
     8041 gtcagcggta tattctggtg actcaaaaga gaccgcaacg tggctcattg cgcccaggtt
     8101 gtacacttca tccggctgta cttcacgcaa aatgcgcgtc aggttagagg tatcactcag
     8161 gtcgccataa tgcagatgga atttcgggtt gcaggtgtgc ggatcctgat aaatgtgatc
     8221 cacgcgctcg gtgttgaatg acgatgcgcg acgcttaata ccatgcacct cgtaaccttt
     8281 ttccagcaga aactctgcca ggtaagaacc gtcttgtccg gttacaccgg tgatgagagc
     8341 gacttttgac atgtcttatt cctctgtatt tttgaattta ttcagtttca acgcgttcgc
     8401 gtatcaccac tgcgggattc ccccggcaaa ccacatttgc cggaagcgat ttaaaaacac
     8461 tgcttcgtgc acccacgacg gtgccgtcgc cgattgtgac gcctggggca acaaagacat
     8521 cggttgccag ccagcatttc tcgccaatca caataggcgt ggcgttaatg gtgaaatgtt
     8581 gacttgcatg gtcgtggcta ccggtgcata aataactttt ttgcgatatc accgaatgtg
     8641 cgccaatggt tatttcaccg agggtatata aattgacgtc atcgccgacc cacgcgtaat
     8701 cacctaaggt taatttccac ggataggtaa tttttactga cggacgaata actacgtttt
     8761 ttcctatttt tgctccgaat aaacgtaata aaaaagcccg ccagcgatac aatacttgtg
     8821 gcgaccaggc aaatattgtt gcctgtactg cccaccataa ttgcacttta atagcgttgc
     8881 cgccccggaa ccctttcggc accgagaaac cgcttaaatc ttgcattggt tttccttata
     8941 ttcagacttt gttatataag gctttcgtct ttgaggtagt acgttggcgt aaatgccagg
     9001 ataattcagc ccaaaagcca ggcacatgta atatttgtcg ttggactttt ttcgcgtcag
     9061 cacacaattc catattattg gtggtagata ccccacccat ggaaaattca gacaccaggc
     9121 cattgagttt tttaaatgca taaccggctt tatacatttt ggctgccagc gcgtagtcgg
     9181 aagaaacttt atattccagg tcataacgcc attttttcaa gccggataca gggaaaaata
     9241 tcgcctgatg actggcgggc aggctgtgat aaatatacca gcccggtttg gcgctacgtt
     9301 taattttatg cccgtcgcca aaatccagca gcgcatcgcc ggtgatcatc atgttatctt
     9361 tttgcatttt taacttacgg acaaaatttg cggcatcctg atgaaaaata tcgcccgaat
     9421 tgagaaacaa cgcgaacttg ccttgtgcca tcgtaatgcc tttgttcatg gcgtcgtaga
     9481 taccgttatc tggctcgctg acaaagcgta ggttatagat accattgaga ttttccagat
     9541 actcacgggt gccgtcattg gaaccgccat cgacaacaat ccattcgaag ctgatatctt
     9601 ccgcctgcgc cagatgcgcc agcgaggcat gtgttttgac tatcccttcg aggttacgaa
     9661 acgcgacagt gattatgcta agcaacatgt tcttattctt acctcgtaat atttaacgct
     9721 tttcgcaaaa taaacggaca gacgattaaa aatgcatatt ccgggctaaa tatcgaaccg
     9781 gtaaaaaaca gcgataccgg agtaaaaaga tataattgca cgcgaaaatt gcggttatca
     9841 ccaaaagcgt tgatcatcat ttttatcact ttccccatgt accacagcga taaaaacact
     9901 gcgaaccagg aaaaataaat aatcagcaga tacaaaccat tgtctatggt ttttccgaca
     9961 tccgcaccgt taaatattcc gaatgatgcg acgtattcat aaagtgagcc aaatctgact
    10021 acaccgtcaa tatgggtcaa agaataaccg accatcacca gcggaccgac aatacgataa
    10081 tacgatgacg atccttccgt acctaaatcg cccagacggg tggaaatata aggaaacgcg
    10141 attaccacac caacccggaa tacagccaga gaaatcaatg ccaacggtaa ctttttctta
    10201 atcgcctctt tattcagata ttgaaatgcc cactccagca aataaaacag gataaaagtc
    10261 ataacccctg aaaacgatcc ggataatatt atccctgcga gaatcatagc atcggtttta
    10321 ggcgttttga taccaaactg tttgatgctg agccaaattg agattaatgc cagagcgaaa
    10381 aatgccggtt cgaaataaag tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagt
    10441 acatagctgt tgctgtaaat cagatatttc gaaatcgact ccataatact gctgccaccg
    10501 gtgaggataa tttgcgccat ctccaccgcc gccagcgcca ccaccagccc gatcaccaga
    10561 taaaagaaac gtaatatctt gcgatggttg tgtggcgaaa tcgttttaaa gcgaatactc
    10621 cacaccatgc caataatgat cacaatatag acaaacagca tggttgaagt gacgtattta
    10681 ctggcatcca gcgactgacc aaacagatag ttaaacgccg tgagtcccgc accaatccct
    10741 aaggcaatca tcaatttttt aacgctgatg cgttctaaaa acagcagtaa caagacaggt
    10801 aaaaaagtga cgatggtgat ggggaaactt tcgccaagct gggcgatttt gacgttaacc
    10861 agcaggtaga tcagcggcag cagcaggtag ctacagattc tgatagaagt tgacatactc
    10921 ctccagcatc tgttgtccac tgtaggtggc gcggctgcgt tggctgaact cagccagcgt
    10981 ggtaccaaat atcgcctgcg cgatttccgg tttgcttaac tgcaccagtt gcagcacgtc
    11041 ttcttcgtcg acggttttac cgccggattt ttgcaacact tcccgtgccg cgtcgctatg
    11101 ggtggcaatc accggcacgc caattgataa cgcctcacac aaaatcagcg ggtagttatc
    11161 gacgcgagaa ctgaatacca gcgcatccat ctgattgagc gcgctcatca acttgcgctt
    11221 gtcagtttca aagccgtgat taaccacgtt gccagcggtg aacggcgaga acttaccaaa
    11281 ggtatgcagt tcgattttgt cgcccagcgc catcatctca cgcaccagtt gctggttagt
    11341 cttgccgtcg taacgcaagt catgcgccac caccgcgatt ttcggcttgc cctgggtttc
    11401 gcgcaccgga ggcaagtccg ccagaatcgc ttcggttgcc atatcaatac cattattgat
    11461 aatccggcaa cgccctggac cgtacaggct attgaaagcg tcagccacat gctggctggg
    11521 ggaaataaac tgacagccca gcgccagcat ctcacggaat aactggcgtt tgcccgccac
    11581 cagctggtgt gcgcgatcaa tcttcaccgg cggatagtta tttaaggtcg ggcatttctg
    11641 gcagcccgtt ttccagcctt cgcaaccgtc ggtaaaggcg cagcgtccgg taacactcca
    11701 gtggtcgtgc agcgtccaga ccagagtgac gtccggttta tggtttttca ctttttcgca
    11761 aaagcgcacc acgctcttaa gatttagcca gtagctgtgc agcacatgaa aatgcaggac
    11821 caccggaccc ggtgtgcgag taatggtgcg atataactca ttgaaattgc caaacagatc
    11881 gcgattaaac agacgaaaca gggcaatatt cgccatcgcg gtcatccgcg gcgtatgttt
    11941 gatgacctgc ggatagttct gatggctgac gctctctttg ccgcctttgc cataaccgta
    12001 gacaaaatgt gacgccagcc cctgttgcag cgcacgctgg tggagatcta acgccacacc
    12061 tgctgccccg ccttccgcca gtcgcacatt aaattgcaga atattcattt aattaccttc
    12121 actcgcgctt tttctcccac caccagcgcg ttgtccggga cagagtcgag caccacgctg
    12181 cccgcaccca cggtgacgtt gttaccaagc gtgatatcac caagaataat gacgttggca
    12241 ccgagttcga cgccgttgcc aatgtgtgga catgccatgt tatcggcacc acgattgccg
    12301 atagtgacgc cgtggcgaat ggtgaaatca tcccccgcta ccacgttttt attgatcact
    12361 acggcgtaac cgtgatggat agtaaagcgg cggccaatgg tcgcggcggc ctggatttca
    12421 taaccgaaaa agcattcggt gataatgcga tacagcacca gcagcggggc cgcccacaga
    12481 ttgttgagga cgttcttttt gcgccacacc gaacaaaaat gagcaacacg ataggcaaga
    12541 accatgcagc acgggcgtaa actccagctg ttggcacgca gatcttccag catctttagc
    12601 gcccccgaat accatcagcc agacgtttgc cgttacgcac cgacagcagc gtcagcaaag
    12661 tgcgccaggt catacgttta ttgcggatct ggtagagggt aaacagctga tattttttgc
    12721 ttgcacggtc aaatttatct ttgtgctttc ggtaaaagtg gaagtacccg gagaattttt
    12781 tcggtgacga ggtgatctgc atttcaccgt gattgatatg caggatctgc gtcgcttctt
    12841 ccactttcca cggttcgccg tactccacca ccatccgcag gaagatgtcg taatcctgtg
    12901 ctgctttcag ttcggtatcg aacaaacact ctttgaaacg ccatgcccaa gtaaagacct
    12961 gattgccaat gatattgcgt ttatagaaca ggcggcgtga gtacggcgat ttgggataca
    13021 gtggtaagct tgccggttgg gaataaactt ctccctggca tacgtagtcg ttagcgtaca
    13081 aaaaggcgtg agtgaccagt tgctgtttat gggcgaggaa gacgctcaga cggttgggtg
    13141 tccattcgtc atcgtcatcg atcccggtaa tgtattcccc ttgcgccagc ataatcgcct
    13201 ggttacgtac cgcgcacgcc ccgctgttaa tgtcgttgtg aatgtaggtg atacgcggat
    13261 cgttgagggc agtgacgtac tgttgcagtt gttcccagga ggtggagcaa tcatccacga
    13321 tgatcatctc ccagttgctg tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca
    13381 gttgttgacg gttccaggtc ggcatataga ttgaaatcag cggattctct ttcataggtt
    13441 gttctcccgg cttatgagct ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc
    13501 tacggggcgg tgcgaatgca ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta
    13561 atagccataa tcctgatacg cgctggcgcg gcggaagatg gagttcagaa tcaccccttt
    13621 caccggaata ccgttttgct caaagcggct cagactggtt tccacttctt tcaatgtgtt
    13681 gaccgcatag cgcgccacca ttaacgtggt tccagcgtga cgcccgacaa ttgctgcatc
    13741 ggtcactgcc agaatcggcg gcgtatcaat caacaccagg tcgtagtttt tactcgccca
    13801 gctcaccaat tcggcaaagc gttcgctcat caacagttca gaaggatttg gcggcacctg
    13861 accgcgcggg atcaggtcaa atttggcaat agaggtcggt ttagctgcgg tagtaatatc
    13921 gccctggcca atcagaattt ccgacaggcc attgacgtta ttggtaccca acagctcgtg
    13981 ggtgtagcct ttgcgcatat cgcagtcgat caacaacacc cgtttattgg tctgactgat
    14041 caccgccgcc aggttggcgc agacaaaggt tttaccgatt gacgggctca cccctgtcat
    14101 catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc agactggtac gcagactgcg
    14161 gatggcttca atcgccagat cggttggatt ccccaccgcc agtagctggc tctgtttata
    14221 gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct ttctgccatt ccgacagcgg
    14281 gatgctggca tagacgctga taccatgttc ttccagcacc tgcgggcttt cgatgccgcg
    14341 attaaacaac gagcgcagca gcacacccac gatagagagc atcaggccga ggataattgc
    14401 gccgaggata atcagtcctt tcttcggttt cagcacgcca ggctgggtga ttgccgggtc
    14461 aacaatgcgc acatcgccga cggtgctggc ttcggtgatt ttcagctcct gctctttatt
    14521 cagcagttgc atatagacct gctgaccaga ctcgacatcg cgggtcaggc gcacaatctc
    14581 ctgctgggtt ttcggcatcg ccgttacgcg accgttaagt ttggctttct cttcttccag
    14641 cgcctgacgt ttctccagca gcgtgcggta cgccgggtga actttggtgt acagcttgga
    14701 gatttccgcc tctttaaagg tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc
    14761 gagcaccgct ttcgcttcca gcggcagatc aacagaatct ttatcctgac ggaaggcatt
    14821 cagtttgttt tcggcaacat cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa
    14881 agcgaggctt ttcgacgctt ccgccgattt gcgctcaata ttttgttcct gatagttacg
    14941 ggcgatgctg ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat aagtcaggct
    15001 cagtacgcct gcgtctttgc cgttctccgt taccgtcagg ctgttttgca gttggttgat
    15061 catccccaac gtggagtatt tggtgacggt aaactcactg cccgggcggg cgtgaatggc
    15121 ttcaaccatc agcgtgacgc cttctttttt cagcatctgg cccgcttgcc cacgggcgct
    15181 aaagccgcca tcactgctca gggtgtagtt tttattgtcc agcacattaa gcgtaaacac
    15241 ctggcccgcc atctctttcg ggcggttaaa ggtagtcact ttcaccgtct cgttctggcg
    15301 tcccatcagt cgctcccagc ccgccccgaa aatcgggaac gtgtttttgc tcactgcaat
    15361 atcgaggtcg agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa tcaactggat
    15421 ctcggcgtcc gatgcaggcg gtttgttggc taatgccgaa ccaatgtcct gcactaacga
    15481 attgccgctg ttttgctcga tttgtaccag tgcgtcggca ctataaatcg gcgttgcgaa
    15541 gaaggtgtaa accacggcac agagggcaaa tacagcggtg atgccaatca cccaccagcg
    15601 cgcttcaatg acggtgccga ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg
    15661 agcggcatgt tgttttactt tttctgtcat tcttatacct gctctgcgtt caatgcctgc
    15721 gcccactggc gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt
    15781 ttgcgatacg gatcggggat ttcacattcg ttatcccagt gaccaaacag catcactttg
    15841 ccgcgcatct caggtgccat ctcgcataag cgttcgatat ggcgcttttc catggtcaaa
    15901 atcaggtcat agttgcgaca caggcggcgg ctgatttgtc gtgcacagtg gccttccaga
    15961 gacagttgat gttctgcggc gacgctgata gcggtaggat cagcgccctt accgaccagc
    16021 gcgccgagtc cagcggactc cactttcagt tccgggtgat aacgttgcag taagcgttcc
    16081 gccgtcgggg aacggcaaat attgccgaca cagacaacta atatgttgtt aaacatgacg
    16141 attaccagtt atgaatgtcg ctggctgtat ccgtcatgta acggacaccg ctaatagttg
    16201 gcaacaactg attgatcaga cggttccagc gggaaaccgg ggcggtggtg acatacacca
    16261 cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag cgacgtggca tcggacatat
    16321 ccagctggta gatattggca atcttgccat tacgcccgcc ctcgcctttc agcggacgaa
    16381 tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat accttcagca ttacccaggg
    16441 cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc ttcacttcac
    16501 ccatcacaaa tactttcaga tcatcattac gcggcacgta gagaatatcg ccgggataaa
    16561 gcagtcggtt ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa atgcgttctt
    16621 cacgaccatt gtgtgttagc actacgttgc gccagtcagc ggtgtcggtc aggccacctg
    16681 cggcgttgat ggcgtcgaga atagtcagtg gcacgttggt gatcgcctgt tgaccggatt
    16741 tattcacctg accggagata taggcctttt gtgagcggaa ggcggcgata ttaacgtcca
    16801 cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat atcactgcgg atttcagcga
    16861 gcgttttccc gactacgtga accttgccga tatacgggta aaacatagtg ccgtcaggct
    16921 gtacccagtt gccggtgtcg ctggagctgc ggtactgacc ggctggcgtg gtgagttccg
    16981 ggtgatccca gacggtgaca ttcagaacgt cgcccggccc gacgcgatac tggtaattcg
    17041 cgatctcgct ttccagcgtc atattggggc gcgctacatt cgggcgtggg cgtaattggt
    17101 caatcaggcg cggggtcagc ggataaacat tcaccatttt gtcgagatcg aaatcagcgt
    17161 cctgctgttt gatgacgtct ttgcccatcg tcgacatatt gctgcccgga agtactgtgc
    17221 aaccgcttat caaggttact gacaccaata atggcatcaa tttcattttg gatttcatca
    17281 ttgtttattt atcactttgg cagagtaatt atcctgtgca ctattaatag caatatcgcc
    17341 atgcacattt accttgcagt taattgaata aaaatttaac tggcatcagt cctaaaaaaa
    17401 ttgatttcat ccgcaggcta ttgacagaat aattcagact ggtctttcag gcatccagac
    17461 acgctaccgc ccctggcttt ttagccacca acacactgat ttagcttaat ttttcaaacc
    17521 ctctctgcat acaatcgttg atgagaaagg gttattgcgg aaattaactt ccgaatataa
    17581 ggtgacatta tggtaattga atattggctc tccaataatg caggaggaag tgttacagct
    17641 aacggaatag caggcaaggt aacaattcgg caattggcta tttt
//
LOCUS       DAZSBF010000074        17623 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZSBF010000074 DAZSBF010000000
VERSION     DAZSBF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.075"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHXWD_001955"
     CDS             <1..245
                     /locus_tag="ACHXWD_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR7620883.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHXWD_001956"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHXWD_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR7620884.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHXWD_001957"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHXWD_001957"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR7620885.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHXWD_001958"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHXWD_001958"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR7620886.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHXWD_001959"
     CDS             3423..4061
                     /locus_tag="ACHXWD_001959"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR7620887.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHXWD_001960"
     CDS             4066..4842
                     /locus_tag="ACHXWD_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR7620888.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHXWD_001961"
     CDS             4931..6295
                     /locus_tag="ACHXWD_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR7620889.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALCASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHXWD_001962"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHXWD_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR7620890.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHXWD_001963"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHXWD_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR7620891.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHXWD_001964"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHXWD_001964"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR7620892.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHXWD_001965"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHXWD_001965"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR7620893.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHXWD_001966"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHXWD_001966"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR7620894.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHXWD_001967"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHXWD_001967"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR7620895.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHXWD_001968"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHXWD_001968"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR7620896.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHXWD_001969"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHXWD_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR7620897.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHXWD_001970"
     CDS             complement(12844..13167)
                     /locus_tag="ACHXWD_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR7620898.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHXWD_001971"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHXWD_001971"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR7620899.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHXWD_001972"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHXWD_001972"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR7620900.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHXWD_001973"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHXWD_001973"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR7620901.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHXWD_001974"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHXWD_001974"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR7620902.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT         4146 a         4720 c         4450 g         4307 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgtgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca cta
//
LOCUS       DAZSBF010000075        17525 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZSBF010000075 DAZSBF010000000
VERSION     DAZSBF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.076"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(76..1359)
                     /gene="gltA"
                     /locus_tag="ACHXWD_001975"
     CDS             complement(76..1359)
                     /gene="gltA"
                     /locus_tag="ACHXWD_001975"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR7620903.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1565..1672)
                     /locus_tag="ACHXWD_001976"
     CDS             complement(1565..1672)
                     /locus_tag="ACHXWD_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620904.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2053..2457
                     /gene="sdhC"
                     /locus_tag="ACHXWD_001977"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2053..2457
                     /gene="sdhC"
                     /locus_tag="ACHXWD_001977"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR7620905.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2451..2798
                     /gene="sdhD"
                     /locus_tag="ACHXWD_001978"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2451..2798
                     /gene="sdhD"
                     /locus_tag="ACHXWD_001978"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR7620906.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2798..4564
                     /gene="sdhA"
                     /locus_tag="ACHXWD_001979"
     CDS             2798..4564
                     /gene="sdhA"
                     /locus_tag="ACHXWD_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR7620907.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4580..5296
                     /gene="sdhB"
                     /locus_tag="ACHXWD_001980"
     CDS             4580..5296
                     /gene="sdhB"
                     /locus_tag="ACHXWD_001980"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR7620908.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5597..8398
                     /gene="sucA"
                     /locus_tag="ACHXWD_001981"
     CDS             5597..8398
                     /gene="sucA"
                     /locus_tag="ACHXWD_001981"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR7620909.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8413..9630
                     /gene="odhB"
                     /locus_tag="ACHXWD_001982"
     CDS             8413..9630
                     /gene="odhB"
                     /locus_tag="ACHXWD_001982"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR7620910.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9724..10890
                     /gene="sucC"
                     /locus_tag="ACHXWD_001983"
     CDS             9724..10890
                     /gene="sucC"
                     /locus_tag="ACHXWD_001983"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR7620911.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10890..11759
                     /gene="sucD"
                     /locus_tag="ACHXWD_001984"
     CDS             10890..11759
                     /gene="sucD"
                     /locus_tag="ACHXWD_001984"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR7620912.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11863..12585)
                     /gene="mngR"
                     /locus_tag="ACHXWD_001985"
     CDS             complement(11863..12585)
                     /gene="mngR"
                     /locus_tag="ACHXWD_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR7620913.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12754..14670
                     /gene="mngA"
                     /locus_tag="ACHXWD_001986"
     CDS             12754..14670
                     /gene="mngA"
                     /locus_tag="ACHXWD_001986"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR7620914.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14688..17321
                     /gene="mngB"
                     /locus_tag="ACHXWD_001987"
     CDS             14688..17321
                     /gene="mngB"
                     /locus_tag="ACHXWD_001987"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR7620915.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            17346..>17525
                     /locus_tag="ACHXWD_001988"
     CDS             17346..>17525
                     /locus_tag="ACHXWD_001988"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620916.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICG"
BASE COUNT         4229 a         4367 c         4776 g         4153 t
ORIGIN      
        1 ttgtgtttgg gcatataaaa attaacccgc catttgaacg gcgggttaaa atatttacaa
       61 cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt catatcctgt
      121 atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc tccagtgggc
      181 gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag acggaatacc
      241 catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt tcttctcgat
      301 aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca gcaggtcatc
      361 tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg tggcgcgcgg
      421 gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat catttttatc
      481 tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt ccagcatttt
      541 cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa tacctgctgc
      601 gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg aggtagaggc
      661 gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt ccagaatcgg
      721 attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt taccggcata
      781 ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt aacacatcgc
      841 ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacggt gacgaggatt
      901 gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac cacacatgac
      961 tgccattgga tgcgagtcgc gacggaaagc atggaacaga cgggtaatct gctcgtggat
     1021 catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag tcggtttttc
     1081 accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca gctgatcgat
     1141 cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa ttttagattc
     1201 gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg aaccgagagt
     1261 acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt caacagctgt
     1321 gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc ttagcgcctt
     1381 attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc tttacccgtt
     1441 tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag ctctgggcgc
     1501 ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt agcaatccga
     1561 attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat acttccacac
     1621 tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc attatatgct
     1681 tttcctggca atgtttgtaa caactttgtt gaatgattgt caaattagat gattaaaaat
     1741 taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg acaaactata
     1801 tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc tccggaacac
     1861 cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg aagcagtgtt
     1921 ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac cggaggaagg
     1981 aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc cccagggaat
     2041 aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca aagacctgtt
     2101 aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat tctccatcgc
     2161 gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct gggtaccagc
     2221 ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt cttcgtcaaa
     2281 tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg tattcgccac
     2341 atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg ctccgccaaa
     2401 atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt atggtaagca
     2461 acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc gctaccgcta
     2521 tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt ggcgaactga
     2581 catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc accctgctgg
     2641 cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg accgactacg
     2701 ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca ctggtggttt
     2761 acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag tcagagaatt
     2821 tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc aaatttccca
     2881 gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc ataccgtttc
     2941 tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact gggaatggca
     3001 tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga ttgaatatat
     3061 gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc cgttctcgcg
     3121 tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa acttcggcgg
     3181 cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc ggtcacgcac tgttgcacac
     3241 gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt atgcgctgga
     3301 tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca tcgaaaccgg
     3361 tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg cagggcgtat
     3421 ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca tggctatccg
     3481 cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg gcattgccgg
     3541 tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc tgaacaaaca
     3601 tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg gccgtgatgt
     3661 ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg gtccgtgggg
     3721 gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat cccgtctgcc
     3781 gggtatcctg gagctttccc gtacctttgc tcacgtcgat ccggtgaaag agccgattcc
     3841 ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga ccggtcaggc
     3901 gctgacggtg aacgagaaag gcgaagatgt ggttgttccg gggctgtttg ccgttggtga
     3961 aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc tgctggacct
     4021 ggtggtcttt ggtcgtgcgg caggtctgca tctgcaagag tctatcgccg aacagggcgc
     4081 attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctgc accgctggaa
     4141 caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag aatgtatgca
     4201 gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg agcagttgaa
     4261 agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg agttcaacac
     4321 ccagcgcgtt gagtgcttgg aactggataa cctgatggaa acggcgtatg caacggctgt
     4381 ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact tcccggatcg
     4441 tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat ccatgacgcg
     4501 ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga ttcgtactta
     4561 ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata acccggatgt
     4621 tgatgatgct ccgcgtatgc aggattaccc cctggaagcg gaagaaggtc gcgacatgat
     4681 gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt tccgccgctc
     4741 ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga atggtctggc
     4801 ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga ttcgcccgct
     4861 gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct atgcgcaata
     4921 tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc gcgagcattt
     4981 acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc tctgcgcatg
     5041 ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg gcccggcagg
     5101 cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg acagccgcct
     5161 cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga actgcgtcag
     5221 tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt cgatgttgtt
     5281 gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat caggcaacca
     5341 gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt aggcctgata
     5401 agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact gcccctgaca
     5461 ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc tcgcaagtga
     5521 accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc ataaaaaaga
     5581 tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact cttcttacct
     5641 ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa ccgatcctga
     5701 ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg gagtcaaacc
     5761 ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag acgcttcacg
     5821 ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag tcctgcagct
     5881 cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc tgggactgtg
     5941 gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg aagcagactt
     6001 ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaacatga aactcggcga
     6061 gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt atatgcacat
     6121 taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc gcgcgacttt
     6181 caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag gccttgaacg
     6241 ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg gtgacgcgtt
     6301 aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc gcgaagtggt
     6361 tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg gtaaaaaacc
     6421 gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca cgggtgacgt
     6481 gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg tgcacctggc
     6541 gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg gttctgttcg
     6601 tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa tcaccatcca
     6661 cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca tgtcgaaagc
     6721 gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg ttggtttcac
     6781 cacctctaat ccactggatg ctcgttctac accgtactgt actgatatcg gtaagatggt
     6841 tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct ttgtgacccg
     6901 tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc tggtgtgcta
     6961 ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc tgatgtatca
     7021 gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg agcaggaaaa
     7081 agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg cgctggatgc
     7141 tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca cctggtcgcc
     7201 gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga agcgcctgca
     7261 ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt ctcgcgttgc
     7321 caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg actggggcgg
     7381 tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc gcctgtcggg
     7441 tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca accagtctaa
     7501 cggttccact tacacgccgt tgcaacacat ccataacggg cagggcgcgt tccgcgtctg
     7561 ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg ccaccgcaga
     7621 accacgcact ctgaccatct gggaagcgca gttcggtgac ttcgccaacg gtgcgcaggt
     7681 ggttatcgac cagttcatct cttctggcga acagaaatgg ggccggatgt gtggtctggt
     7741 gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg cgcgtctgga
     7801 acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgttccgt ctaccccggc
     7861 acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc cgctggtagt
     7921 gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg aagaactggc
     7981 gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga agggcgtgaa
     8041 gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc gtcgtaagaa
     8101 caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc cgcataaagc
     8161 gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt gccaggaaga
     8221 gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag tgattccgtt
     8281 tggggcttct ctgcgttatg caggccgcac ggcctccgcc tctccggcgg tagggtatat
     8341 gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg tcgaataaat
     8401 aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga atccgtagcc
     8461 gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg tgatgaagtg
     8521 ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc agacggcatt
     8581 ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat ccttggtcgc
     8641 ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga gaaagcgtcc
     8701 actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc gttaagcccg
     8761 gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa aggcaccggt
     8821 gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc cccggcgaaa
     8881 gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc acgtagtgaa
     8941 aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct ggaagcgaaa
     9001 aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat tatggatctg
     9061 cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg ctttatgtcc
     9121 ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa tgcttctatc
     9181 gacggtgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt ttccacgccg
     9241 cgcggcctgg tgacgccggt actgcgtgat gtcgataccc tcggcatggc agacatcgag
     9301 aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt tgaagatctg
     9361 accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat gtctacgccg
     9421 atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga tcgtccgatg
     9481 gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc ctacgatcac
     9541 cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga gttgctggaa
     9601 gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca ctgtagaccg
     9661 acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg atggacagaa
     9721 cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg cttaccagca
     9781 ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc aaaaatcggt
     9841 gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa agcgggcggt
     9901 gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg gctcggcaag
     9961 cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat tctggttgaa
    10021 gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg tagttcccgt
    10081 cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt ggcggaagaa
    10141 actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat gccgtatcag
    10201 ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca gttcaccaaa
    10261 atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat cgaaatcaac
    10321 ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact gggcgctgac
    10381 ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc gcaggaagat
    10441 ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga cggtaacatc
    10501 ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt taaactgcac
    10561 ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga acgtgtaacc
    10621 gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt taacatcttc
    10681 ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt agcagaagtg
    10741 ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact cggcgcgaag
    10801 aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga tgcagctcag
    10861 caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa aacaccaagg
    10921 ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag gccattgcat
    10981 acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc cacctcggcc
    11041 tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc gcttctgtta
    11101 tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat gcaggcatca
    11161 aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact gtgaaagtga
    11221 aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt atcactccgg
    11281 gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa gtgggtatcg
    11341 tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat tacggtttcg
    11401 gtcagtcgac ctgtgttggt atcggcggtg acccgatccc aggctctaac tttatcgaca
    11461 tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc ggtgagatcg
    11521 gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc aagccagttg
    11581 taggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac gcgggtgcga
    11641 tcattgctgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa gccgcaggcg
    11701 tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt ctgaaataaa
    11761 tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga agggctgtcg
    11821 gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg atgattttcg
    11881 ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc tatattcaaa
    11941 cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct ttgtctggct
    12001 gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt cctgttcgct
    12061 acgatcgata accatcttct tcacttcttc aataaagtga tatttcgaat tttccatgac
    12121 ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca gcgccattgg
    12181 cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg cggtgatttg
    12241 tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga tttcactgtg
    12301 tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa aaatatcgta
    12361 attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga ggatctgctg
    12421 ctcgactagc tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa actctgtttg
    12481 tagcgctgat tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg caatttgttc
    12541 acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta gtatctcatt
    12601 aatatgaatt tagccattat gcccgataaa ttcatcctgt aaataataca aatacaatac
    12661 aaataatttc aatcaagtga aattgatcac atgatggtat tgttttatcg ggcacactgg
    12721 cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct gacccaccgc
    12781 gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca cgcgttgact
    12841 caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga agaagtgtat
    12901 cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa
    12961 actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg
    13021 gaaggcgttg atggcccgga agcagttgat ttagtggtgc tgctggcgat tccccccaat
    13081 gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct tgcggatgat
    13141 gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc ggcgctggat
    13201 gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat cgtctgcgta
    13261 acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct ggaaaaagcc
    13321 ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg
    13381 cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc tgaagtcgcc
    13441 atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt tgccgagccg
    13501 attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg
    13561 cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct gaaacaggca
    13621 ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg
    13681 gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga agaaaactcc
    13741 tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat ggtaccggtg
    13801 ctcgcggcct ataccaccta ttctctggca gataaaccgg cgttagcgcc aggctttgcg
    13861 gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt tggcggattg
    13921 atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag taaattcaat
    13981 ggattcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg cagtctgatg
    14041 ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact cgcttaccgc ctggctgaac
    14101 ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat gtgttccttt
    14161 gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc aatggcgaac
    14221 ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc atttaccgta
    14281 accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga gaccgggaaa
    14341 tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat ggcgattgaa
    14401 gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg cgctattgtc
    14461 ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct ctttttactt
    14521 catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc ggcattggtg
    14581 ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt taagcatggc
    14641 aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg aaagcggtat
    14701 ctcgcgttca catcaccccg catatgcatt gggatcggga gtggtatttc accaccgaag
    14761 agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg gaacaggaca
    14821 acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg
    14881 tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga agccggtaag ttgattatcg
    14941 gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc gtccgtaatc
    15001 tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac
    15061 cggattcctt tggcatgtcc gggcaactgc cgcatattta caatggattt ggcattaccc
    15121 gcactatgtt ctggcgcgga tgttcggagc gccacggtac tgataaaacc gagtttttgt
    15181 ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc tacgccatcg
    15241 gtaagtactt acctgccgac gaaaacggat tacgtaaacg cctcgacagt tattttgacg
    15301 tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatggtcat gaccagatgc
    15361 cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac cctcaacgta
    15421 agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag cgtgaaagtc
    15481 tggccaccct gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat cgcaccatcg
    15541 gttccacgcg catggatatc aaaattgccc acgcacgtat tgaaaataag attgttaatc
    15601 tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac cacggcttgc
    15661 tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc ggttgttgtt
    15721 gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga actggctgaa gacatggcgg
    15781 ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag agtgatgccg
    15841 acaaactcgt gctgtttaac ctgatgccct ggccgcggga agaagttatt aacaccactg
    15901 tgcggctgcg cgccagccag tttaatctac aagacgatcg cggtcagcct gtaccgtatt
    15961 ttattcgcca tgcccgtgag atcgatcctg gcctaatcga tcggcaaatt gttcattatg
    16021 gcaattatga tccctttatg gagtttgata ttcagatcaa ccagattgtc ccttctatgg
    16081 gctatcgcac gctttatatt gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg
    16141 acgctgaagg gattctggaa aatgctttct ggcagattgc gctcaatgag gatggttctc
    16201 tgcaactggt agataaagac agcggtgtgc gctatgaccg ggtattgcaa attgaagaaa
    16261 gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg gtaattaccg
    16321 cagcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc ctggcagagc agggctgtta
    16381 tccgctatga catggcagtg ccgctcaatt tgtcagaacg cagcgcccgg caatccactg
    16441 gcagagtagg gggggtgttg gttgtcactc ttagtcataa cagcaggcgt attgatgtgg
    16501 atatcaacct tgataaccag gctgacgatc atcgccttcg tgtcctggtc cctacacctt
    16561 ttaacactga cagtgttctg gcagatacgc agtttggttc gctaacgcgc cccgtgaacg
    16621 acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt ccggtatgga
    16681 atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg catggctgtc tttagcgaag
    16741 ggttacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt acgttgctgc
    16801 gtggcgtggg cttactgggc aaagaagatc tgcttttaag gcctgggcgg ccttcgggaa
    16861 ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc ctaagtttat
    16921 tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga gcatggctga
    16981 ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac aaagccggat
    17041 tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc ctgataagcg
    17101 cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat ccggctgaat
    17161 cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc tcagaaacga
    17221 tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg gggccttttt
    17281 tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc aaaccgaagc
    17341 cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga tacggcatga
    17401 acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat gatgtagaca
    17461 ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag ccacgtatat
    17521 gtggc
//
LOCUS       DAZSBF010000076        17448 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZSBF010000076 DAZSBF010000000
VERSION     DAZSBF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.077"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /locus_tag="ACHXWD_001989"
     CDS             complement(<1..502)
                     /locus_tag="ACHXWD_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7620917.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="ACHXWD_001990"
     tRNA            complement(688..772)
                     /locus_tag="ACHXWD_001990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="ACHXWD_001991"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="ACHXWD_001991"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR7620918.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHXWD_001992"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="ACHXWD_001992"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR7620919.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="ACHXWD_001993"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="ACHXWD_001993"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR7620920.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="ACHXWD_001994"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="ACHXWD_001994"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR7620921.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="ACHXWD_001995"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="ACHXWD_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR7620922.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="ACHXWD_001996"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="ACHXWD_001996"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR7620923.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="ACHXWD_001997"
     CDS             7117..8619
                     /locus_tag="ACHXWD_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR7620924.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHXWD_001998"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="ACHXWD_001998"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR7620925.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHXWD_001999"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="ACHXWD_001999"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR7620926.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHXWD_002000"
     CDS             complement(10983..11033)
                     /gene="ytgA"
                     /locus_tag="ACHXWD_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR7620927.1"
                     /translation="MSIIILATISTTQENK"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="ACHXWD_002001"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="ACHXWD_002001"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR7620928.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="ACHXWD_002002"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="ACHXWD_002002"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR7620929.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="ACHXWD_002003"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="ACHXWD_002003"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR7620930.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="ACHXWD_002004"
     CDS             complement(16186..17382)
                     /locus_tag="ACHXWD_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR7620931.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDATYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4338 c         4453 g         4174 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa ccctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg cgggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagcatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAZSBF010000077        17337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZSBF010000077 DAZSBF010000000
VERSION     DAZSBF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.078"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..970
                     /gene="ybeQ"
                     /locus_tag="ACHXWD_002005"
                     /pseudo
     CDS             1..970
                     /gene="ybeQ"
                     /locus_tag="ACHXWD_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
     gene            complement(1040..1522)
                     /locus_tag="ACHXWD_002006"
     CDS             complement(1040..1522)
                     /locus_tag="ACHXWD_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR7620932.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1757..4339
                     /gene="leuS"
                     /locus_tag="ACHXWD_002007"
     CDS             1757..4339
                     /gene="leuS"
                     /locus_tag="ACHXWD_002007"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR7620933.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4354..4935
                     /gene="lptE"
                     /locus_tag="ACHXWD_002008"
     CDS             4354..4935
                     /gene="lptE"
                     /locus_tag="ACHXWD_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR7620934.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4935..5966
                     /gene="holA"
                     /locus_tag="ACHXWD_002009"
     CDS             4935..5966
                     /gene="holA"
                     /locus_tag="ACHXWD_002009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR7620935.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            5968..6609
                     /gene="nadD"
                     /locus_tag="ACHXWD_002010"
     CDS             5968..6609
                     /gene="nadD"
                     /locus_tag="ACHXWD_002010"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR7620936.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6633..7244
                     /locus_tag="ACHXWD_002011"
                     /pseudo
     CDS             6633..7244
                     /locus_tag="ACHXWD_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7504..7821
                     /gene="rsfS"
                     /locus_tag="ACHXWD_002012"
     CDS             7504..7821
                     /gene="rsfS"
                     /locus_tag="ACHXWD_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR7620937.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7825..8292
                     /gene="rlmH"
                     /locus_tag="ACHXWD_002013"
     CDS             7825..8292
                     /gene="rlmH"
                     /locus_tag="ACHXWD_002013"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR7620938.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8323..10224
                     /gene="mrdA"
                     /locus_tag="ACHXWD_002014"
     CDS             8323..10224
                     /gene="mrdA"
                     /locus_tag="ACHXWD_002014"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR7620939.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10227..11339
                     /gene="mrdB"
                     /locus_tag="ACHXWD_002015"
                     /gene_synonym="rodA"
     CDS             10227..11339
                     /gene="mrdB"
                     /locus_tag="ACHXWD_002015"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR7620940.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11350..12438
                     /gene="rlpA"
                     /locus_tag="ACHXWD_002016"
     CDS             11350..12438
                     /gene="rlpA"
                     /locus_tag="ACHXWD_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR7620941.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12577..13788
                     /gene="dacA"
                     /locus_tag="ACHXWD_002017"
     CDS             12577..13788
                     /gene="dacA"
                     /locus_tag="ACHXWD_002017"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR7620942.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13898..14161
                     /gene="ybeD"
                     /locus_tag="ACHXWD_002018"
     CDS             13898..14161
                     /gene="ybeD"
                     /locus_tag="ACHXWD_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR7620943.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14262..14903
                     /gene="lipB"
                     /locus_tag="ACHXWD_002019"
     CDS             14262..14903
                     /gene="lipB"
                     /locus_tag="ACHXWD_002019"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR7620944.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15163..16116
                     /locus_tag="ACHXWD_002020"
     CDS             15163..16116
                     /locus_tag="ACHXWD_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR7620945.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16325..17290
                     /gene="lipA"
                     /locus_tag="ACHXWD_002021"
     CDS             16325..17290
                     /gene="lipA"
                     /locus_tag="ACHXWD_002021"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR7620946.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4322 a         4386 c         4452 g         4177 t
ORIGIN      
        1 ttacgtcaag ttgctgcgat aatttatcaa tagatgagat tatcgaacgt gctgaaaaag
       61 gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc gcaattgact
      121 ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa ggtcattgtg
      181 aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat aaagataatg
      241 agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc gcttccaacg
      301 cccttggctg gacactggat cgtggagaag cccccaatta taaagaagcg gttgtctggt
      361 atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg tggatgtaca
      421 gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac aaacaagctg
      481 cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa gacggaaaag
      541 gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca cagcagggta
      601 atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg gtggaccagg
      661 actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc gtcggcgctt
      721 acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat tatcaggctg
      781 cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg tataacctgg
      841 ccattattat cactacggag aaggaagacc tgtcgatctc cgacaggctc tcgacctgta
      901 tcgtaaagtt cagtcatccg gaaccagaga tgtcagtcag gaaattcgtg agactgaaga
      961 tttactgtag agataaaaac aagggccggg gaatttcatt cggcccatat tctctttatt
     1021 gctgtgcgaa atcagactat tacggctcaa acgggcgtct ctggaactgg tcatgaccac
     1081 atttcgggca tagcgtcagc acttccggtg tgtagatcgg gagatggaag tgacatttct
     1141 cgcagaccag atttcccagc ccgaccactt ctccgctgtg ataaacccca tgatgattga
     1201 ggtcctggaa aacttcgcgc cattcaagct gcgttttatc ggtgatatct gccagctcct
     1261 gccataagct ttctttaatc acccgcataa agacgctgtc agattcttct ttcaggctct
     1321 cttcatagct catggcgaac tcttccaggt cacgtcttac agctcgcgtc agctcatcga
     1381 cctcggttcg cgttaactcc cctgttttta ttacgcgctc gcgcgcctgt tccaccagtg
     1441 cgtcgatatc acgttcgcca ttgcgcaggc gttcgctcag tgacgcgacc agttcacggt
     1501 aatattgagc aaccttgttc atcgtttcat ctcccgggtc gttaactgac tataataata
     1561 gacgttcttt gttccttgct ttgctaatac gcccacaaac tctgtaaatt aatgtatgaa
     1621 taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct gttttgacgc ggacgtttgg
     1681 gctatgctat gcggatctga aaaaccacct caacgctaca tttgtagccg tattgaaaac
     1741 aggaccactg gctgccatgc aagagcaata ccgcccggaa gagatagaat ccaaagtaca
     1801 gcttcattgg gatgagaagc gcacatttga agtaaccgaa gacgagagca aagagaagta
     1861 ttactgcctg tctatgcttc cctatccttc tggtcgacta cacatgggcc acgtacgtaa
     1921 ctacaccatc ggtgacgtga tcgcccgcta ccagcgtatg ctgggcaaaa acgtcctgca
     1981 gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc gcggcggtga aaaacaacac
     2041 cgctccggca ccgtggacgt acgacaacat cgcgtatatg aaaaaccagc tcaaaatgct
     2101 gggctttggt tatgactgga gccgcgagct ggcaacctgt acgccggaat actaccgttg
     2161 ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg gtatataaga agacttctgc
     2221 ggtcaactgg tgcccgaacg accagaccgt actggcgaac gaacaagtta tcgacggctg
     2281 ctgctggcgc tgcgatacca aagttgaacg taaagagatc ccgcagtggt ttatcaaaat
     2341 cactgcttac gctgacgagc tgctcaacga tctggataaa ctggatcact ggccagacac
     2401 cgttaaaacc atgcagcgta actggatcgg tcgttccgaa ggcgtggaga tcaccttcaa
     2461 cgttaacgac tatgacaaca tgctgaccgt ttacactacc cgcccggaca cctttatggg
     2521 ttgtacctac ctggcggtag ctgcgggtca tccgctggcg cagaaagcgg cggaaaataa
     2581 tcctgaactg gcggccttta ttgacgaatg ccgtaacacc aaagttgccg aagctgaaat
     2641 ggcgacgatg gagaaaaaag gcgtcgatac tggctttaaa gctgttcacc cattaacggg
     2701 cgaagaaatt cccgtttggg cagcaaactt cgtattgatg gagtacggca cgggcgcagt
     2761 tatggcggtt ccgggtcacg accagcgcga ctacgagttt gcctctaaat acggcctgaa
     2821 catcaaacca gttatcctgg ctgctgacgg ctctgagcca gatctttctc agcaagccct
     2881 gactgaaaaa ggcgtgctgt tcaactctgg cgaattcaac ggtcttgacc atgaagcggc
     2941 cttcaacgcc atcgccgata aactgactgc gatgggcgtt ggcgagcgta aagtgaacta
     3001 ccgcctgcgc gactggggtg tttctcgtca gcgttactgg ggcgcgccga ttccgatggt
     3061 gacgctggaa gacggtaccg taatgccgac cccggacgac cagctgccgg tgatcctgcc
     3121 ggaagatgtg gtgatggacg gcattaccag cccgattaaa gcagatccgg agtgggcaaa
     3181 aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac actttcgaca cctttatgga
     3241 gtcctcctgg tactatgcgc gctacacttg cccagagtac aaagaaggta tgctggattc
     3301 cgaagcggct aactactggc tgccggtgga tatctacatt ggtggtatcg aacacgccat
     3361 tatgcacctg ctctacttcc gcttcttcca caaactgatg cgtgatgcag gcatggtgaa
     3421 ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg gtgctggcag atgccttcta
     3481 ctatgttggc gaaaacggcg aacgtaactg ggtttccccg gttgatgcta tcgttgaacg
     3541 tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca ggccatgaac tggtttatac
     3601 cggcatgagc aaaatgtcca agtcgaagaa caacggtatc gacccgcagg tgatggttga
     3661 acgttacggc gcggacaccg ttcgtctgtt tatgatgttt gcttctccgg ctgatatgac
     3721 tctcgaatgg caggaatccg gtgtggaagg ggctaaccgc ttcctgaaac gtgtctggaa
     3781 actggtttac gagcacacag caaaaggtga tgttgcggca ctgaacgttg atgcgctgac
     3841 tgaagatcag aaagcgctgc gtcgcgatgt gcataaaacg atcgctaaag tgaccgatga
     3901 tatcggccgt cgtcagacct tcaacaccgc aattgcggcg attatggagc tgatgaacaa
     3961 actggcgaaa gcaccaaccg atggcgagca ggatcgcgct ctgatgcagg aagcgctgct
     4021 ggccgttgtc cgtatgctta acccgttcac cccgcacatc tgcttcacgc tgtggcagga
     4081 actgaaaggc gaaggcgata tcgacaacgc gccgtggccg gttgctgacg aaaaagcgat
     4141 ggtggaagac tccacgctgg tcgtggtgca ggttaacggt aaagtccgtg ccaaaatcac
     4201 cgttctggtg gacgcaacgg aagaacaggt tcgcgaacgt gctggccagg aacatctggt
     4261 agcaaaatat cttgatggcg ttaccgtgcg taaagtgatt tacgtaccag gtaaactcct
     4321 caatctggtc gttggctaag cgcgggagga agcgtgcgat atctggcaac attgttgtta
     4381 tctctggcgg tgttaatcac cgccgggtgt ggctggcatc tgcgtgatac cacgcaggtt
     4441 ccttccacta tgaaggtcat gatcctggac tcaggcgatc cgaacgggcc attaagccgt
     4501 gcagtgcgta accagttacg tctgaatggt gtcgagttgc ttgataaaga aaccacgcgt
     4561 aaggacgttc catccctgcg tttgggtaaa gtgagcatcg cgaaagatac cgcatcggta
     4621 ttccgtaacg gtcaaacagc agagtatcag atgatcatga cggtgaatgc gaccgtgttg
     4681 atccccggcc gtgatatcta cccgattagc gccaaagtct tccgttcgtt ctttgataac
     4741 ccgcaaatgg cgttagcgaa agataacgaa caagacatga tcgtaaaaga gatgtacgac
     4801 cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc gtgctgcgga tattcgttcc
     4861 gacgaagaac agacgtcgac cacaacggat actccggcaa cgcctgcacg cgtctccacc
     4921 acgctgggta actgatgatt cggttgtacc cggaacaact ccgcgcgcag ctcaatgaag
     4981 ggctgcgcgc ggcgtatctt ttacttggta acgatcctct gttattgcag gaaagccagg
     5041 acgctgttcg tcaggtagct gcggcacaag gattcgaaga acaccacact ttttccattg
     5101 atcccaacac tgactggaat gcgatctttt cgttatgcca ggctatgagt ctgtttgcca
     5161 gtcgacaaac gctattgctg ttgttaccag aaaacggacc gaatgcggcg atcaatgaac
     5221 aacttctcac actcaccgga cttctgcatg acgacctgct gttgatcgtc cgcggtaata
     5281 aattaagcaa agcgcaagaa aatgccgcct ggtttactgc tcttgcgaat cgcagcgtgc
     5341 aggtgacctg tcagacaccg gagcaggctc agcttccccg ctgggttact gcgcgcgcaa
     5401 aacagctcaa cttagaactg gatgacgcgg caaatcaggt gctctgctac tgttatgaag
     5461 gtaacctgct ggcgctggct caggcactgg agcgtttatc gctgctctgg ccagacggca
     5521 aattgacatt accgcgcgtt gaacaggcgg tgaatgatgc cgcgcatttc accccttttc
     5581 attgggttga tgctttgttg atgggaaaaa gtaagcgcgc attgcatatt cttcagcaac
     5641 tgcgtctgga aggcagcgaa ccggttattt tgttgcgcac attacaacgt gaactgttgt
     5701 tactggttaa cctgaaacgc cagtctgccc atacgccact gcgtgcgttg tttgataagc
     5761 atcgggtatg gcagaaccgc cggggcatga tgggcgaggc gttaaatcgc ttaagtcaga
     5821 cgcagttacg tcaggccgtg caactcctga cacgaacgga actcaccctc aaacaagatt
     5881 acggtcagtc agtgtgggca gagctgaaag ggttatctct tctgttgtgc cataaacccc
     5941 tggcggacgt atttatcgac ggttgatatg aaatctttac aggctctgtt tggcggcacc
     6001 tttgatccgg tgcactatgg tcatctaaaa cccgtggaaa cgctggcgaa tttgattggt
     6061 ctgacgcggg tcacaatcat ccctaataat gttcctccgc atcgtcccca gccggaagcg
     6121 aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg ccgacaagcc attatttact
     6181 cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca ctgcgcaaac actgaaagag
     6241 tggcggcagg aacaaggacc ggacgtgccg ctggcgttta ttattggtca ggattcactg
     6301 ctgacctttc cgacctggta cgaatacgaa acgatactcg acaatgcaca tttgatcgtc
     6361 tgtcggcgtc caggttaccc acttgaaatg gcgcaaccgc aataccagca atggctggaa
     6421 gatcatttga cacataaccc ggaagatctt caccttcagc ctgccggtaa aatttatctg
     6481 gctgaaacgc cgtggtttaa catctcggcg accatcatcc gcgaacgttt gcaaaacggt
     6541 gaatcgtgtg aggatttatt gccggaaccg gtactgactt acattaacca acaaggcttg
     6601 tatcgctgat acctgtcgtt acggagggca tgatgcgact gtggttaatt cgtcatggtg
     6661 aaacgcaagc gaatatcgat ggtctttaca gcggtcatgc gcccaccccc ctgaccgcgc
     6721 gcggtattga gcaagcgcaa aatctgcata cgctgctaca tggtgtttcc tttgatctgg
     6781 ttttatgcag tgaactggaa cgggcacagc ataccgcacg actggttctc agtgaccgcc
     6841 agctccccgt gcaaatcatc cctgaactca acgaaatgtt ttttggcgac tgggagatgc
     6901 gacatcatcg cgacctcatg caagaagatg ccgaaaacta tagcgcgtgg tgcaatgact
     6961 ggcagcatgc aatccccacg aacggtgaag gatttcaggc attttcgcaa cgtgtggaac
     7021 gctttatcgc aaggcttagt gaatttcagc actatcagaa tattttagtc gtcagccatc
     7081 agggtgtact gagtctgtta atcgcccgtt taattggcat gcctgccgag gctatgtggc
     7141 attttcgcgt tgactagggg tgttggagcg ccattgatat caaccaaaaa ttcgcgacgc
     7201 tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc tgttttttta
     7261 cgggtaagcc gcaacgacca ttgacagacc cgggcaggct gatattctcc gcagccagac
     7321 ttttttcgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat gcaatctgcg
     7381 gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt gtcccgaaat
     7441 cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac ccagggggaa
     7501 aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga cctcaaaggt
     7561 caggacatca tcgccttaga cgttcaaggc aaatccagca tcaccgactg catgatcatc
     7621 tgtacgggta cgtccagccg tcatgttatg tccattgctg accacgttgt gcaggagtct
     7681 cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga ctggattgtc
     7741 gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg cctgtatgaa
     7801 ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg aacgaaaatg
     7861 ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa agatatgccc
     7921 ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat caagcgcata
     7981 ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat tgtcaccctc
     8041 gatattccag gcaagccctg ggatacgccg cagttagccg ctgagctgga acgctggaag
     8101 ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc gcctgcctgt
     8161 aaagcggcgg cggagcagag ctggtcgctg tcggcgctta ccctccccca tccgctggtt
     8221 cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa ccatccttat
     8281 caccgtgagt gataagggag ctttgagtag aaaacgcagc ggatgaaact acagaactct
     8341 tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt cgcctttttg
     8401 gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca aattgttcgc
     8461 tttaccgact accagactcg ctctaatgaa aaccgcatta agctggtgcc tatcgcgccc
     8521 agccgcggca ttatctacga ccgtaatggt atccctctgg ccctcaaccg cactatctac
     8581 cagatagaaa tgatgccaga gaaagtcgat aacgtgcagc aaacgctgga cgctttgcgc
     8641 agcgtggttg atctgaccga tgacgatatt gctgcattcc gaaaagagcg cgcacgttca
     8701 caccgtttca cctctattcc ggtgaaaact aacctgaccg aagtacaagt agctcgcttt
     8761 gccgtcaatc agtaccgttt tccgggtgtc gaagttaaag gctataaacg tcgttactat
     8821 ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa cgataaagac
     8881 gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca tgatatcggt
     8941 aagctgggca ttgagcgtta ctatgaagac gtgctgcacg gtcagaccgg ttatgaagag
     9001 gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc accgcaagcc
     9061 ggacacgata tttacctgac gctggatctc aaactccagc aatatattga aacgctgctg
     9121 gcgggtagcc gcgcagctgt ggttgtcacc gatccgcgta caggtggggt gctggcgctg
     9181 gtttccacgc ctagttatga cccaaacctg tttgttgacg gtatctccag caaagattat
     9241 tccgccttgt tgaacgcccc gaatacaccg ctggtgaacc gcgccacaca gggggtttat
     9301 cctcccgcgt ctacagttaa accctatgtg gcggtttcgg cattgagcgc cggggtgatc
     9361 acgcgcaata cgacgctgtt tgacccaggc tggtggcaac tgccaggttc ggaaaaacgt
     9421 tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc gctggaagaa
     9481 tctgcggata ccttcttcta tcaggtggcc tacgatatgg ggatcgatcg cctctccgaa
     9541 tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga agaacgttcc
     9601 ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg gtatcagggt
     9661 gacaccattc cggttggtat cggtcagggt tactggacag cgaccccaat ccagatgagt
     9721 aaggcactga tgatcctgat taatgacggt atcgtgaagg ttcctcattt gctgatgagc
     9781 accgccgaag acggcaaaca ggtgccatgg gtacagccgc atgaaccgcc cgtcggcgat
     9841 attcattccg gttactggga gctggcgaaa gacggtatgt acggtgttgc taaccgccct
     9901 aacggtacgg cgcataaata ctttgccagc gcaccgtaca aaattgcggc gaaatccggt
     9961 accgctcagg tcttcggtct gaaagcgaac gaaacctata atgcgcacaa aattgccgag
    10021 cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc gcaagtggct
    10081 gtcgccatga ttctggagaa cggtggtgcg ggtccggcgg ttggtacact gatgcgccag
    10141 atcctcgacc acattatgct gggtgataac aacaccgatc tgcctgcgga aaatccagcg
    10201 gttgccgcag cggaggacca ttaatcatga cggataatcc gaataaaaaa acattctggg
    10261 ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg gtttacagcg
    10321 ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt aaaatcggcc
    10381 aaatcgcgat gggtctagtc atcatggtgg tgatggcgca aattcctcca cgcgtttatg
    10441 aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg gtagatgctt
    10501 tcggtgccat ctctaaaggt gctcaacgct ggctggacct cggtattgtt cgttttcagc
    10561 cgtcggaaat tgccaaaata gccgtaccac tgatggttgc gcgctttatc aaccgcgacg
    10621 tttgcccgcc atcgttgaag aacactggca tcgcgctggt gctgatattt atgcccacgc
    10681 tgctggtggc tgcacagcct gacctgggaa catcaatcct cgttgcgctt tccggtctgt
    10741 ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta gtgctggtgg
    10801 cggcgttcat tccgattctg tggttcttcc tgatgcatga ttaccagcgc cagcgcgtaa
    10861 tgatgctcct agacccggaa tcagacccac tcggcgcggg ctatcacatt attcagtcta
    10921 aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcacggc actcagtcac
    10981 agcttgaatt tctccccgaa cgccataccg actttatctt cgcggtactg gcggaagagc
    11041 tgggattagt gggcattctg attctgctcg ctctctacat tctgctgatc atgcgcgggc
    11101 tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggt ggcttaatgc
    11161 tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt ctgccggttg
    11221 taggggttcc gctcccactg gtcagttatg gaggatcggc gctaattgtg ctgatggctg
    11281 ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa agcgtgtaag
    11341 aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat gctcgcggca
    11401 tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc ggtatgtaac
    11461 ggccccatag ttgaaattag cggggcggac ccgcgtttcg aaccactgaa cgcgacggca
    11521 aatcaggatt accagcgcga cggtaaaagc tacaaaatcg tgcaggatcc gtctcgattt
    11581 agccaggcgg gactggcggc aatctatgat gccgaacccg gcagtaacct gacggcctct
    11641 ggcgaagctt tcgatccgac acagctgacg gcggcccatc caacgcttcc gatccccagc
    11701 tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat taatgatcgc
    11761 ggcccttacg gcaacgaccg cgttatttca ctttctcgcg cggcagctga ccgtcttaac
    11821 acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga tggttcgctt
    11881 tctggtcctg gtatggcttg taccacagtc gccaaacaga cttacgccct gccagcacct
    11941 cccgatttaa gcggtggcgc gggaacaagt tcagtgtctg gcccgcaggg tgacattctt
    12001 ccggtcagta attcgacgct aaaaagcgaa gatccgaccg gcgcgccggt aaccagtagc
    12061 ggtttcctcg gcgctccaac gaccttagcg cctggtgtac tggaaggcag cgaaccgacg
    12121 cctgcgccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc gcctgcaatg
    12181 gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca agtcggggcc
    12241 gtaagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa gttcggcgtc
    12301 cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc agcttggccc attcgccagc
    12361 aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt acagtcattt
    12421 attactaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg taaatgacgt
    12481 taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat agtagggcac
    12541 ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt ttccgctcgt
    12601 atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc tgctgcacat
    12661 gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga tgcggagtcc
    12721 tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc agatgtccgc
    12781 cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca ggcaatgaaa
    12841 gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg ggccaccggt
    12901 aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca ggttccggtt
    12961 tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt cgccatggcc
    13021 gattttgccg ctggtagcca ggacgctttt gttggcttga tgaacagcta cgttaacgca
    13081 ctgggcctga aaaataccca cttccagacg gtacatggtc tggatgctga tggtcagtac
    13141 agctccgcgc gagatatggc gctgatcggc caggcattga tccgtgacgt accgaatgaa
    13201 tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct gaaccgtaac
    13261 ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca cactgacaaa
    13321 gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat ttctgcggtg
    13381 atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgct aacctggggc
    13441 ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc ctctgaaccg
    13501 gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt gtacctgacc
    13561 attccacgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag cagtgaattg
    13621 catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct tgatggcaaa
    13681 acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa cttcttcggc
    13741 aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa attgaacact
    13801 tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaactccc gccttctggc
    13861 gggagttgct atttaattac gttacgccgg agctgacatg aaaaccaaac ttaacgaact
    13921 gcttgaattc cctactcctt ttacttacaa agttatgggg caggcgttac ctgagctggt
    13981 tgatcaggtg gttgaagtgg tacagcgcca tgcgccaggt gactacaccc caacggtaaa
    14041 accaagcagc aaaggcaact accactcggt atctatcact atcaacgcca ctcatatcga
    14101 gcaggttgaa acactgtatg aagaactagg caaaatcgat attgtccgca tggttctgta
    14161 actcgcttct ccgttacccg gcccctggtc gggtaactcc cctctcctcg ctgtgatata
    14221 cttttccccc acttttactc attctccacg gagatgcagt tttgtatcag gataaaattc
    14281 ttgtccgcca gctcggtctt cagccttacg agccaatctc ccaggctatg catgaattca
    14341 ccgatacccg cgatgatagt acccttgatg aaatctggct ggtcgagcac tatccggtat
    14401 tcacccaagg tcaggcagga aaagcggagc acattttaat gccgggtgat attccggtga
    14461 tccagagcga tcgcggtggg caggtgactt atcacgggcc ggggcaacag gtgatgtatg
    14521 tgttgcttaa cctgaaacgc cgtaaactcg gtgtgcgtga actggtgacc ttgcttgagc
    14581 aaacagtggt gaataccctg gctgaactgg gtatagaagc gcatcctcgg gctgacgcgc
    14641 ctggtgtcta tgtcggggaa aagaaaattt gctcactggg tttacgtatt cgacgcggtt
    14701 gttcattcca cggtctggca ttaaacgtca atatggatct ttcaccattt ttacgtatta
    14761 atccttgtgg gtatgccgga atggaaatgg ctaaaatatc acaatggaaa cccgaagcga
    14821 cgactaataa tattgcacca cgtttactgg aaaatatttt agcgctacta aacaatcctg
    14881 acttcgaata tattaccgct taattgcata cctcaatggc ccaatttaca ctgggtcatt
    14941 actcataata cctgctaatg ctttacatct ccttcgcgtt tgtatacttt ctttatcact
    15001 tgcagaaatg cggtgctacg ccttgctccg taactcaccc cattgattaa cctattgaaa
    15061 tagttgatta tggtgagcaa aagcgttccg ttccaattag acataagatt attgtaattt
    15121 ttttaaatat tatttttcca tgaataaaat tggagtaagt gcgtggatag taataatcaa
    15181 attgaaccct gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt tacaacctta
    15241 cgaaatattg atcttaacct tctgactatt tttgaagctg tatatgtaca taaagggatc
    15301 gttaatgcag cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc tattcagaaa
    15361 ctgcgcgtta tattccctga cccattgttt attcgcaaag gccagggtgt cactcctacc
    15421 gcatttgcga tgcatctaca tgagtatatc agtcagggcc ttgagtccat tcttggcgcg
    15481 ctggatatcg aaggaagcta tgataagcaa cgaacgataa ctattgcgac cactccctcg
    15541 gtcggagccc tggtccttcc tgtcatctat cgggcgatta aaactcacta tccgcagctt
    15601 ttactgcgca acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt tcaaaccgat
    15661 ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt tctgttcacc
    15721 gacaatatgg tgttaatttg ccgcgaggga aatccactac tctctttaga agatgacaga
    15781 gagactatcg acaacgctgc gcatgtactc ctgttaccgg aagggcaaaa tttcagcggt
    15841 ctgcggcaga gagttcaaga gatgtttccg gaccggcaaa tcaatttcac cagctacaac
    15901 attttgacaa tcgctgcact ggttgccaac agtgacatgt tagcgattat tcccagccgt
    15961 ttttataacc tgtttagtcg ttgctggccg ctggaaaaac tgccttttcc gtccttaaat
    16021 gaggagcaaa tagatttctc tatccactac aacaaattca gcctgcgtga tcccatcctg
    16081 cacggggtaa tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca
    16141 cagcgcagaa cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt
    16201 ggctgctttc aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat
    16261 aattacaaca atctattgaa ttgaaacgct ttccttcgta attcgcaact ggaacacgca
    16321 cgctatgagt aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat
    16381 ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga
    16441 atggatgaaa atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat
    16501 gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg
    16561 cttcaaccac ggcacagcaa cgtttatgat cctcggcgct atttgtaccc gccgttgtcc
    16621 gttctgtgat gttgcccacg gtcgcccggt agctcctgat gccaatgaac cagtgaaact
    16681 ggcgcagacc attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga
    16741 tgacctgcgc gatggcggtg cccagcactt tgcggattgc attactgcca ttcgggaaaa
    16801 aagcccgcaa atcaaaattg aaactctggt gccggatttc cgtggtcgta tggatcgtgc
    16861 tctggatatt ctgactgcaa cgccaccaga tgtgttcaac cataacctgg aaaacgtacc
    16921 gcgtatttac cgtcaggtac ggcctggtgc agattacaac tggtcgctga agctgctgga
    16981 acgctttaaa gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggactggg
    17041 tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat
    17101 gttaacgctg gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt
    17161 tagcccggat gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc
    17221 tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga
    17281 agttaagtaa tcctgtaata attacttaca tctgtctgat aaaaaacccg ctttttg
//
LOCUS       DAZSBF010000078        17008 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZSBF010000078 DAZSBF010000000
VERSION     DAZSBF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.079"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHXWD_002022"
     CDS             <1..266
                     /locus_tag="ACHXWD_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR7620947.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHXWD_002023"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHXWD_002023"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR7620948.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHXWD_002024"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHXWD_002024"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR7620949.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHXWD_002025"
     CDS             complement(1570..3270)
                     /locus_tag="ACHXWD_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR7620950.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQEQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHXWD_002026"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHXWD_002026"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR7620951.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHXWD_002027"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHXWD_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFR7620952.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="ACHXWD_002028"
     CDS             4828..6600
                     /locus_tag="ACHXWD_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR7620953.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHXWD_002029"
     CDS             6593..8374
                     /locus_tag="ACHXWD_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR7620954.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHXWD_002030"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHXWD_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR7620955.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHXWD_002031"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHXWD_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR7620956.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHXWD_002032"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHXWD_002032"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR7620957.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHXWD_002033"
     CDS             11336..11908
                     /locus_tag="ACHXWD_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR7620958.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHXWD_002034"
     CDS             complement(11939..12250)
                     /locus_tag="ACHXWD_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR7620959.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHXWD_002035"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHXWD_002035"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHXWD_002036"
     CDS             12629..12982
                     /locus_tag="ACHXWD_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR7620960.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHXWD_002037"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHXWD_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHXWD_002038"
     CDS             complement(14736..15206)
                     /locus_tag="ACHXWD_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR7620961.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHXWD_002039"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHXWD_002039"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR7620962.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHXWD_002040"
     CDS             complement(16044..16262)
                     /locus_tag="ACHXWD_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR7620963.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHXWD_002041"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHXWD_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR7620964.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         4061 a         4123 c         4554 g         4270 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc tcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcct
//
LOCUS       DAZSBF010000079        16936 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZSBF010000079 DAZSBF010000000
VERSION     DAZSBF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.080"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHXWD_002042"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHXWD_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR7620965.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHXWD_002043"
     CDS             2219..2767
                     /locus_tag="ACHXWD_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR7620966.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHXWD_002044"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHXWD_002044"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR7620967.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHXWD_002045"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHXWD_002045"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR7620968.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHXWD_002046"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHXWD_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR7620969.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACHXWD_002047"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="ACHXWD_002047"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR7620970.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="ACHXWD_002048"
     CDS             complement(8301..9503)
                     /gene="pncB"
                     /locus_tag="ACHXWD_002048"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR7620971.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9769..12381
                     /gene="pepN"
                     /locus_tag="ACHXWD_002049"
     CDS             9769..12381
                     /gene="pepN"
                     /locus_tag="ACHXWD_002049"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR7620972.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="ACHXWD_002050"
     CDS             complement(12588..13355)
                     /gene="ssuB"
                     /locus_tag="ACHXWD_002050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR7620973.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="ACHXWD_002051"
     CDS             complement(13352..14143)
                     /gene="ssuC"
                     /locus_tag="ACHXWD_002051"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR7620974.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="ACHXWD_002052"
                     /pseudo
     CDS             complement(14155..15302)
                     /gene="ssuD"
                     /locus_tag="ACHXWD_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="ACHXWD_002053"
     CDS             complement(15299..16258)
                     /gene="ssuA"
                     /locus_tag="ACHXWD_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR7620975.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="ACHXWD_002054"
     CDS             complement(16251..16826)
                     /gene="ssuE"
                     /locus_tag="ACHXWD_002054"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR7620976.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
BASE COUNT         4225 a         4402 c         4318 g         3991 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcagtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggaacttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagaatg cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcggggttg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcctcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tggggggggg gatacctatg ttacctggca tctgcaatca
     8221 gacaagcaga attcgcaaat gcagcgaaag atttcggaat taaaggtaaa aaaagggagc
     8281 cgattagctc cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg
     8341 cgcagcgccc gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt
     8401 ttcgccaccg gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc
     8461 tgggggatat cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag
     8521 aagtggcggt atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt
     8581 ttactctgtg gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca
     8641 accgggtcgc cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa
     8701 tcacgcagga aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga
     8761 tactcttcca gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt
     8821 tgctgatgtg cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga
     8881 cgcgccagat cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg
     8941 atggtttctt gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag
     9001 cgcgacatat caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg
     9061 tcgagggctt gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata
     9121 accgccagca aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata
     9181 tccagcttgc cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt
     9241 aaccagttaa gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca
     9301 tcctgcaggc gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata
     9361 cccagcagat cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga
     9421 tgaaacacgg cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga
     9481 acaggagaag cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt
     9541 acaataaaca tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg
     9601 atcacaccat atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt
     9661 agcaataaag gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac
     9721 aggaatagac tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag
     9781 ccacaagcca aataccgtca cgattatcgt gcgccagatt accagattac tgatattgac
     9841 ttgacctttg acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt
     9901 catggtgcat cagatgctcc acttcgtctc aacggcgaag acctcaaact ggtttctgtt
     9961 catattaatg atgagccgtg gaccgcctgg aaagaagaag agggcgcact ggtcatcagt
    10021 aatttgccgg agcattttac gcttaagatc gttaatgaaa taagcccggc ggcgaatacc
    10081 gcgctggaag ggctttatca gtcaggcgat gcgctttgca cccagtgtga agccgaaggt
    10141 ttccgccata ttacgtatta tctcgaccgc ccggacgtgc tggcgcgttt taccaccaaa
    10201 attattgccg ataaaaccaa atatcccttc ctgctttcca acggtaaccg cgttgcgcaa
    10261 ggtgaactgg aaaacggacg ccattgggta cagtggcagg acccgttccc gaaaccgtgc
    10321 tacctgtttg cgctggtggc aggcgacttt gatgtactgc gcgacacctt taccacgcgt
    10381 tctggtcgcg aagtggcgct ggagctgtac gtcgatcgcg gcaaccttga tcgcgcgccg
    10441 tgggcgatga cctcgctgaa aaactcaatg aaatgggatg aagaacgctt cggcctggag
    10501 tatgacctcg acatctatat gatcgtcgcg gtggatttct tcaatatggg cgcaatggag
    10561 aataagggtc tgaatatctt taactccaaa tatgtgctgg cccgcaccga caccgccacc
    10621 gacaaagatt acctcgatat tgaacgcgtt atcggccatg aatatttcca taactggacc
    10681 ggtaaccgag tgacctgccg cgactggttc cagctcagcc tgaaagaagg tttaaccgtc
    10741 ttccgcgatc aggaattcag ctctgacctt ggttcccgcg cagttaaccg catcaataat
    10801 gtgcgcacca tgcgcggatt gcagtttgcg gaagacgcca gcccgatggc gcacccaatc
    10861 cgcccggaca tggtcattga gatgaacaac ttctacaccc tgaccgttta cgagaagggc
    10921 gcggaagtga tccgtatgat ccacaccctg ctgggcgaag aaaacttcca gaaagggatg
    10981 caactctatt tcgagcgtca tgatggtagc gcagcgacct gtgacgactt tgtgcaggcg
    11041 atggaagatg cgtcgaatgt tgatctctcc catttccgcc gttggtacag ccagtccggt
    11101 acgccgattg tgaccgtcaa agacgactac aatccggaaa ctgagcagta caccctgacc
    11161 atcagccagc gcacgccagc cacgccggat caggcagaaa aacagccgct gcatattccg
    11221 tttgccatcg aattgtatga caacgaaggc aaagtgatcc cgttgcagaa aggcggtcat
    11281 ccggtgaatt ccgtgctgaa cgtcacccag gcggaacaga cctttgtttt tgataatgtc
    11341 tacttccagc cggtgcctgc gctgctgtgc gaattctctg cgccagtgaa actggaatat
    11401 aagtggagtg atcagcaact gaccttcctg atgcgtcatg cgcgtaatga tttctcccgt
    11461 tgggatgcgg cgcaaagttt gctggcaacc tacatcaagc tgaacgtcgc ccgtcatcag
    11521 caagggcagc cgctgtctct gccggtacat gtggctgacg ctttccgcgc ggtgctgctc
    11581 gatgagaaga ttgatccggc gctggcggca gaaatcctga cgctgccttc tgtcaatgaa
    11641 atggctgaac tgttcgatat catcgacccg attgctattg ccgaagtacg cgaagcactc
    11701 actcgtactc tggcgactga actggcggat gagctgctgg ctatttacaa cgcgaattac
    11761 cagagcgagt accgtgttga gcatgaagat attgcaaaac gcactctgcg aaatgcctgc
    11821 ctgcgcttcc ttgcttttgg tgaaacgcat ctggctgacg tgctggtgag caagcaatac
    11881 catgaagcaa acaatatgac cgatgcgctg gcagcgcttt ctgcggcggt tgctgcacag
    11941 ctgccttgcc gtgacgcgct gatgcaggag tacgacgata agtggcatca ggacggtctg
    12001 gtgatggata aatggtttat cctgcaagcc accagcccgg cggcgaatgt gctggagacg
    12061 gtgcgtggtc tgttgcagca tcgctcattt accatgagca acccgaaccg tattcgttcg
    12121 ttgattggtg cgtttgcggg cagcaacccg gcagcgttcc atgccgaaga tggcagcggt
    12181 taccagttcc tggaggaaat gcttaccgac ctcaacagcc gtaacccgca ggtagcttca
    12241 cgtctgattg aaccgctgat tcgcctgaaa cgttatgatg ccaaacgtca ggagaaaatg
    12301 cgcgcggcgc tggaacagtt gaaagggctg gaaaatctct ctggcgatct gtacgagaag
    12361 atcaccaaag cactggcttg atacttttga gccaaaatgc ctgatgcgac gcttgacgcg
    12421 tcttatcagg cctacaactc cgttgaaatt gctggttttg taggctggat aaggcgttca
    12481 cgccgcatcc ggcagccgtg cccaactgcc tgatgcgatg cttgacgtgt cttatcaggc
    12541 ctacaagtcc gttgaaaatg ctggttttgt gcgccacatc cggcatatta accatgacgc
    12601 cgaatcagtt gttcagaatg cccacgccgc attacccgtt gcaatacttc cgcttccagc
    12661 tccgctaatc gcaccgaccc taatcgacgc ggacggggga tatccactgt caaatccaga
    12721 ccaatttttc cctcttcaat taacaacacc cggtcagcca ttgctaccgc ttcgctcaca
    12781 tcatgcgtca ccaacagtac ggtaaagccg tgctgctgcc aaagtgacac aatcaaatcc
    12841 tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag caacaataaa
    12901 cccggtcgat gaatcaacgc tcttgccagc gccacacgct gtttctgccc gccagaaagt
    12961 gcggcaggcc attccccggc gcgattctcc agtcctactg cagccaacgc ccgacgcgcg
    13021 gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga tttccacggc
    13081 agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatctcagc cagcggtgtg
    13141 gtgcccgcta acacatcgcc tgcggttggc gtttccagac ctgccagcag gcgcagcagg
    13201 gtacttttgc caccaccgct gcggcccacc accgccacaa actgacctgc cggaatatgt
    13261 aaatccagtt ggttcaggac gatattttcc gcgtaatgtt tgcttactgc attgagcaac
    13321 aatggtgtgc cctggttcag acgagcagta ttcataccgt ggcctccttc aaatgataag
    13381 ccgggttcca gcgcaaccag aggcgctcta acagctgcgc gctgacgtca gcaagtttgc
    13441 cgagcagggc gtaaagaata atagcgacta ccaccacgtc cgtttgcaaa aactcccgcg
    13501 cattcatcgc cagataacca atgcctgaat tggcagaaat ggtttcggca acaatcagcg
    13561 tcagccacat caggcccaac gcaaaacgca cgccgaccat aattgagggc agggcaccag
    13621 gcaggatcac atggataaac agcggtatgc cggataatcc atagctacgc gccatttcca
    13681 ccagaccgcg atcgatatta cggatgccat gccaggtgtt gatataaatg gggaatagcg
    13741 tgccgagcgc caccagaaag atttttgcgg attcatcaat gccaaaccac aaaatcacca
    13801 gcgggatcag cgccagatgc ggtacgttgc gcaacatctg aattgaggta tccagcagcc
    13861 gttctcccca gcgtgacagc ccgctaatca gtcccagaat caagcccagc gatccgccaa
    13921 ttgaaaagcc aatcagcgcc cgccaggaac tgatcgccag atgctgccac aattcgccgc
    13981 tggcggagag cgtccagaac gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg
    14041 acagccagcc aaccgaggag gccagttgcc acaccgccac gatgcccacc ggtaaaaacc
    14101 agggggcaac gcgcaataac cacttcttca ctggcgttgc catcatcgtg ttccttagct
    14161 ttgcgcgact ttacggggga taaaatcatt cgccaccgct tcgccttgcg gattcagcgg
    14221 ctgcggctgg ggaatttccg gaatggcgac atccagatgt gggaacagca actcgccaac
    14281 ccgatacgct tcttccagat gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc
    14341 ggcgtattcg ttgattcgcg cggcgaccgt aggaccatcg cccaccagcg ccgttccggc
    14401 accgccgcgc actaagccaa cgcccgccca taaattgggg ctgatctcca gattgtcgcg
    14461 cttgccgtta tgtagcgccg ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc
    14521 ggcctgtgct ttggcgatag tttcatcatc aagatgcgag attaaccgct cggcggcctg
    14581 ccacgcttcg tcgttagttt cacgaacaat cacatgcaga cgaataccga aacgaatttt
    14641 gcgtccatgc gcggcagctt tcgcccgcac ttgttcgatc ttctctttta ccagctctgg
    14701 cggttcgccc caggtgaggt agagatcaac ttgttctgcc gccagctcct gggcgacatc
    14761 tgacgatccg ccaaagtaaa gcggaggata cggctgttga atcggcggga agagcagttt
    14821 tgctccccgc acatgaatat gtttaccgtt gaaatcgacg ctttccccca gcaataagcg
    14881 ccccgccaga cctgggtaaa ttccgccgag gcttcgtagc gctcgctatg atcaaggaac
    14941 acgccgtcgc ctgccaactc ttgtggatcg ctgcctgtga ccagattaaa caacgcacga
    15001 ccatttgaga gacggtcaag cgtggcggcc tggcgagcgg caacggtagg tgaggttacg
    15061 ctgggacgca gggcgacaag aaacttcagc cgctgcgtca ccgggatcat cgacgccgcc
    15121 accagccacg catcttcaca cgatcgcccc gttgggatca gcacaccggt atagcccaga
    15181 cgatccgccg cttgcgcaat ttgttgcaga tagccgtgat caaccgggcg tgaaccttct
    15241 tccgttccaa gataatgccc gtcaccatgg gtcggtaaaa accagaacat attcagactc
    15301 ataattgttt tccttccagg tgagtgggct gccagatgcg ctggcgaata tcaacttttt
    15361 tcgacaccag acggttttca taaaacagat ctgccgtttg ctgctgtaag gcggcaactt
    15421 cagcgctcac cggtttgatg gtggtgggag gacgatgatc cagatacgag gcaatcaccg
    15481 gtgccggtaa gcccatcgtt tttgccagta aagcgatgct ttgctcgcgc tggctgcggg
    15541 ttaacgcatc ggcctcacta aaggttgcca gtacgccctg aataaaagcg ccgttttttt
    15601 ctgcataggg gcgagctgcc agataaaacg atccagtttg attgagatcg gtgccgtctt
    15661 tcagcacccg cacgccgccc tgtaataacg cagcggagta gtagggatcc cagatagccc
    15721 aggcgtcaac gttaccttgc tggaacgcgg cgcgggcatc agctggcgtc agataagtgg
    15781 gctggatgtc agtaaactta agtccggcct gacgcagtgc acgcagtaaa aggttgtgtg
    15841 aactggaacc tttctgaaag gcaactttgt gacctttaag atcggctacg gttttgatcg
    15901 ggctgttttc tgccaccaga atcacttcgg ctttgggctt cggtggctcg acgcccacat
    15961 atatcaaatc agccccggca gcctgggcaa agattggcgg aatatccccg gtactgccga
    16021 gatcaatact gccaacgttt aacgcttcca acatttgcgg acccgcgggg aactccaccc
    16081 aggagatttt tgattgcgga tagcgttttt ccagtaactg gtggcttttt gccaatacca
    16141 taccaatact gcctttctga tagcctatac gtaacgcttc aggcgaggat tctgcagcaa
    16201 ccgcaaaagt agagacgcta agcaatcccg ccagcgccag tttaatgatg ttacgcatgg
    16261 acattacctc gcagagacag aaggtcagga acctgaacat cgcggcggtg caatgcctgc
    16321 cagaaagttt ctagcgcggt atcaagacgg gtttgcagat ttggcgtgaa ctggggtttg
    16381 tgatggtaat caataacttg tgagtcatcg gcaaacacgc cgtgcaggag ctcctgggct
    16441 ttcatcgcac ttaaaactgg cttaagggca taatcgaccg ccagcagatg agccaccgta
    16501 ccgcccgtcg ccagcggtag caccactttg ccttgcaaag cgcgctctgg cagcaggtcg
    16561 agcagggttt tcagcgcgcc ggaataggcg gctttataca caggtgtagc gactatcagt
    16621 ccatcagcct gttgcagctg ttcggtgaag gtcttgagtg ccggactatc gaaacgagcg
    16681 taaagcagat cttccggggc gaagttttgc agattccagt gataaacctc tacatccagg
    16741 ccatttagtt tttcccgcgc atattccagc aaggagctgg agcgagaagg aaagcgagga
    16801 ctacccgcca gggtgatgac acgcatactc tctccttata accaattgtt ctttttttgt
    16861 taacattgat aacaattcgg tcagtctgtc ggagagacaa gaaaatgcca aatataaatt
    16921 ttgtgtatct ttttct
//
LOCUS       DAZSBF010000080        16883 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZSBF010000080 DAZSBF010000000
VERSION     DAZSBF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.081"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            331..1314
                     /locus_tag="ACHXWD_002055"
                     /pseudo
     CDS             331..1314
                     /locus_tag="ACHXWD_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(1630..2832)
                     /locus_tag="ACHXWD_002056"
     CDS             complement(1630..2832)
                     /locus_tag="ACHXWD_002056"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR7620977.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3064..4563
                     /gene="pitA"
                     /locus_tag="ACHXWD_002057"
     CDS             3064..4563
                     /gene="pitA"
                     /locus_tag="ACHXWD_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR7620978.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4807..5142)
                     /gene="uspB"
                     /locus_tag="ACHXWD_002058"
     CDS             complement(4807..5142)
                     /gene="uspB"
                     /locus_tag="ACHXWD_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR7620979.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5533..5967
                     /gene="uspA"
                     /locus_tag="ACHXWD_002059"
     CDS             5533..5967
                     /gene="uspA"
                     /locus_tag="ACHXWD_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR7620980.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6066..6119)
                     /locus_tag="ACHXWD_002060"
     CDS             complement(6066..6119)
                     /locus_tag="ACHXWD_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620981.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6285..7754
                     /gene="dtpB"
                     /locus_tag="ACHXWD_002061"
     CDS             6285..7754
                     /gene="dtpB"
                     /locus_tag="ACHXWD_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR7620982.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7803..8555)
                     /gene="rsmJ"
                     /locus_tag="ACHXWD_002062"
     CDS             complement(7803..8555)
                     /gene="rsmJ"
                     /locus_tag="ACHXWD_002062"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR7620983.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8563..10605)
                     /gene="prlC"
                     /locus_tag="ACHXWD_002063"
     CDS             complement(8563..10605)
                     /gene="prlC"
                     /locus_tag="ACHXWD_002063"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR7620984.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10808..11650
                     /gene="rlmJ"
                     /locus_tag="ACHXWD_002064"
     CDS             10808..11650
                     /gene="rlmJ"
                     /locus_tag="ACHXWD_002064"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR7620985.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11722..13074
                     /gene="gorA"
                     /locus_tag="ACHXWD_002065"
     CDS             11722..13074
                     /gene="gorA"
                     /locus_tag="ACHXWD_002065"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR7620986.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13128..13211)
                     /gene="dinQ"
                     /locus_tag="ACHXWD_002066"
     CDS             complement(13128..13211)
                     /gene="dinQ"
                     /locus_tag="ACHXWD_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR7620987.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13239..13412
                     /locus_tag="ACHXWD_002067"
     CDS             13239..13412
                     /locus_tag="ACHXWD_002067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7620988.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13951..14304
                     /gene="arsR"
                     /locus_tag="ACHXWD_002068"
     CDS             13951..14304
                     /gene="arsR"
                     /locus_tag="ACHXWD_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR7620989.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14358..15647
                     /gene="arsB"
                     /locus_tag="ACHXWD_002069"
     CDS             14358..15647
                     /gene="arsB"
                     /locus_tag="ACHXWD_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR7620990.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15660..16085
                     /gene="arsC"
                     /locus_tag="ACHXWD_002070"
     CDS             15660..16085
                     /gene="arsC"
                     /locus_tag="ACHXWD_002070"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR7620991.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16219..16515)
                     /locus_tag="ACHXWD_002071"
     CDS             complement(16219..16515)
                     /locus_tag="ACHXWD_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR7620992.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16622..>16883
                     /locus_tag="ACHXWD_002072"
     CDS             16622..>16883
                     /locus_tag="ACHXWD_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR7620993.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3902 a         4381 c         4277 g         4323 t
ORIGIN      
        1 atgatacaaa gcaataatac ttatatgttc gatgatttta agcaccgaga ctatccggtc
       61 ttttactaaa taatggaaaa caatccatcc ctggaagata attgtaaaaa aaacaacaga
      121 ataagaatta atcttaggat aaatatttat ttatcatggc cttttaaccg gtaaaaaatg
      181 gcactcggca cagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc
      241 gtcgttatat taacaggctg aaattatcat taatgcacat ttcattatta gcagggatac
      301 ctgaggagtt taaagtgaac atatatatcg ggtggctttg tgctggaaag ttcagggcaa
      361 agcgagtatt tcgtcgcggg tcatgtgctg atttctctgg cggccatatg cctggcatta
      421 ttcactaccg catttattat catttcgcag ctcacgcgcg gcgttaatac gttttacaat
      481 acattgttcc ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca
      541 ctgttagcag gcaatgatgt gatggcagac gagtttgtcg ccggccatgt tattttcggc
      601 gttggtatga ttgccgcctg tgtatcgacg gtggcagcgt catccggtca ctttctgctc
      661 attcccaaaa atgcagcggg gagcaagagc gatggaacgc cggtacaggc ttattcttca
      721 ttaatcggta attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg
      781 tctattacgc tgttacgtag tgctgacata actccgcatt atgtcgcggg tcacgtattg
      841 cttgggttaa ccgcaatctg tgcctgtcta attggccttg ttgccacaat tgtccatcaa
      901 acacgtaata cgttttcaac taaagaacac tggctgtggt gttattgggt tatttttctc
      961 ggctcaatca cggtactgca ggggatatac gtcttagtca gttccgatgc aagcgcccga
     1021 ctggctcccg gcattattct tatttgcctc ggaatgatct gttacagcat attctcaaaa
     1081 gtctggctac tggcactggt ctggagacgt acctgttcgt tagccaacag aataccgatg
     1141 atacccgtct tcacctgcct gttttgcctt ttcctggcat catttcttgc ggaaatggcg
     1201 cagaccgaca tgggatattt tattccttcg cgagttctgg tcggtttggg agcggtatgc
     1261 tttacgttgt tctcaatcgt ttcaatatta gaagcgggtt ctgctaaaaa ataattgcaa
     1321 cgtaccggat aaaaccagcg ttgaccattt gcgtaacgct ggtttttctt aggcatcatg
     1381 aaataacgca gcattaatgc atagtggtta aggataaaaa aacaacaaag ttactgtctt
     1441 tttcagcctg ttcatatttt ataaagatct ggccactggg tgagttttca atcacctttc
     1501 cttccacctt atattaagcc tggagggttt cagttcacgg gctcattaga aaataatcac
     1561 aagatagcca ctatcaaaat tagctcattt ttaatgcggc tctaataatt ttcatcttta
     1621 ggtaacaggt caggacgact tcgctgcaat caaatcctgc gcacaagccc acgcactcga
     1681 ccacgcccac tggaagttat agccccccag ccagccggta acgtccatca cttcgccgat
     1741 gaagtacagc ccaggcactt tgcgcgcttc catcgtccgt gaagagagtt cgttggtgtc
     1801 cacgccgccg agcgtcactt cggcagtgcg atagccttca gtgccgttgg gttgtacacg
     1861 ccagtcggtc aatgtgctaa tcagtgcctg ttggtcacgc acgttgagct gttttagcga
     1921 aacattcggg atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg gtagatgaac
     1981 cgccagtgtg tttttcaggc tctgattcgg atgtgcgtta cgctgctcat tcaggaaggt
     2041 ttcgagatcc acatccggta gcagattgat actgacaaat tcacccggtt gccagtagct
     2101 ggaaatctgc aacaccgccg gtccagacaa gccgcgatgg gtgaagagta agttctcacg
     2161 gaaaacgatg ccgttttcag cggtaatcac ggaaggcacc gccacgcccg ccagcacctg
     2221 taactcttcg agcaacggtt tatgcagagt gaatggcacc agacccgcac gggtcggcag
     2281 cacgttgagg ccaaattgtt cggcaatctt ataaccaaac ggcgacgcgc ccagccccgg
     2341 cattgacagc ccaccggtcg ccatgaccag cttttcgcaa ccgacagtca tgccgttcag
     2401 atcaagcgtg aagcctgttt catcctccgc cacactcagc acttcgctac gcaatctgaa
     2461 ggtcacattg cccttctcgc actcatccac cagcatgtcg acaatctgct gcgcggagtc
     2521 atcgcagaag agttgcccta acgttttctc gtgccatgcg atgccgtgtt tattgaccag
     2581 atcaatgaaa tcccactggg taaaacgtgc gagtgcagac ttacaaaaat gcggattctg
     2641 gctcagataa gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc caccgccaga
     2701 cataaggatt ttgcgccctg gttttttacc attatcgatc agcagaaccc ggcgtcctgc
     2761 ctgacctgcc agcgcagaac agaacatacc cgccgcacca gcgcctataa taatggcatc
     2821 aaacctttcc acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa agattcctca
     2881 gtggtcgcac cagcatcaat attactaaaa ggaagtattt gcctgaatta tataagataa
     2941 ttattttttg agtgaaatcc atacaggagg caaatcaaaa aaagtctata tttcactttg
     3001 cccgcgccgc gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat ggcgtaacgt
     3061 cctatgctac atttgtttgc tggcctggat ttgcataccg ggctgttatt attgcttgca
     3121 ctggcttttg tgctgttcta cgaagccatc aatggtttcc atgacacagc caacgccgtg
     3181 gcaaccgtta tctatacccg cgcgatgcgt tctcagctcg ccgtggttat ggcggcggtg
     3241 ttcaactttt tgggtgtttt gctgggtggt ctgagtgttg cctatgccat tgtgcatatg
     3301 ctgccgacgg atctgctgct taatatggga tcgtctcatg gccttgccat ggtgttctct
     3361 atgttgctgg cggcgattat ctggaacctg ggtacctggt actttggttt acctgcatcc
     3421 agctctcata cgctgattgg cgcgatcatc gggattggtt taaccaatgc gttgatgacc
     3481 gggacgtcag tggtggatgc actcaatatc ccgaaagtat taagtatttt cggttctctg
     3541 atcgtttccc ctattgtcgg cctggtgttt gctggcggtc tgattttctt gctgcgtcgc
     3601 tactggagcg gcaccaagaa acgcgcccgt atccacctga ccccagcgga gcgtgaaaag
     3661 aaagacggca agaaaaagcc gccgttctgg acacgtatcg cgctgatcct ttccgctatc
     3721 ggcgtggcgt tttcgcacgg cgcgaacgat ggtcagaaag gcattggtct ggttatgttg
     3781 gtattgattg gcgtcgcgcc agcaggcttc gtggtgaaca tgaatgccac tggctacgaa
     3841 atcacccgta cccgtgatgc catcaacaac gtcgaagctt actttgagca gcatcctgcg
     3901 ctgctcaaac aggctaccgg tgctgatcag ttagtaccgg ctccggaagc tggcgcaacg
     3961 caacctgcgg agttccactg ccatccgtcg aataccatta acgcgctcaa ccgcctgaaa
     4021 ggtatgttga ccaccgatgt ggaaagctac gacaagctgt cgcttgatca acgtagccag
     4081 atgcgccgca ttatgctgtg cgtttctgac actatcgaca aagtggtgaa gatgcctggc
     4141 gtgagtgctg acgatcagcg cctgttgaag aaactgaagt ccgacatgct tagcaccatc
     4201 gagtatgcac cggtgtggat catcatggcg gtcgcgctgg cgttaggtat cggtacgatg
     4261 attggctggc gccgtgtggc aacgactatc ggtgagaaaa tcggtaagaa aggcatgacc
     4321 tacgctcagg ggatgtctgc ccagatgacg gcggcagtgt ctatcggcct ggcgagttat
     4381 accgggatgc cggtttccac tactcacgta ctctcctctt ctgtcgcggg gacgatggtg
     4441 gtagatggcg gcggcttaca gcgtaaaacc gtaaccagta ttctgatggc ctgggtgttt
     4501 acccttccgg ctgcggtact gctttccggc gggctgtact ggctctcctt gcagttcctg
     4561 taatcgtacg caccaaaacg agcgggtcag ctggcccgct tcagattgtg acatagtgcg
     4621 ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaaac atgcaaattc
     4681 aatagattgc agagattatg taggcctgat aagcgtagcg catcaggcaa ttttgcgttt
     4741 gtaatcagtc tcgagcgggt cagtttactg gcccgctttt tttatgcgtt attattgctg
     4801 aagtgcttaa tgccaaatca tcaatgcaat caggctgacc accaccagac cacacaatgc
     4861 gctggtcaga ataaactgcc gacgcacccg ctcacagcgg cgaataaatt catcatcgtg
     4921 atgatcgcga taacgttggg catagatata ccaaacgaga cgcacctgtt tgttgggttg
     4981 gccatgtgag gtaaaaaagc cccctccatc aacatattga tagagcaatg gatcgcagtt
     5041 acgcagtacc actaacaacg cgcgtagtga tgagaaatag cgcgccatgt taacaatgca
     5101 aacgacacat aaagcccaaa ataatgcgac ggtgcttatc atacctcctc cccggcgacc
     5161 tgcccgcgga gttccacccc ggggctaccg ctcccgatac gctgccaatc agttaacacc
     5221 aggtcctgga gaaaccgctt ttgtggtgac caacatccga gcggctctat agatagtgta
     5281 ggagatcagg ttgttttttt tccagaaggt taaccactat caatatattc atgtcgaaaa
     5341 tttgtttatc taacgggtaa gcaaggcgga ttgacggatc atccgggtcg ctataaggta
     5401 aggatggtct taacactgaa tctttacggc tgggttagcc ccgcgcacgt agttcgcagg
     5461 acgcgggtga cgtaacggca caagaaacgc tagctggcca gtcatcgaca actttatgga
     5521 aggagtaaca ctatggctta taaacacatt ctcatcgcgg tcgatctctc cccggaaagc
     5581 aaagttctgg tagagaaagc agtctctatg gctcgcccct acaatgcgaa agtttctctg
     5641 atccacgtag atgtaaacta ctctgaccta tacaccgggc ttattgatgt gaatctgggt
     5701 gatatgcaga aacgcatctc tgaagagaca catcatgcgc tgaccgagct ttccactaat
     5761 gcaggctacc caatcactga aaccctgagc ggcagcggcg acctgggcca ggttctggtc
     5821 gatgcaatca agaaatacga tatggatctg gtggtttgtg gtcaccacca ggacttctgg
     5881 agcaaactaa tgtcttccgc acgtcagctg atcaacaccg ttcacgttga tatgctgatt
     5941 gttccgctgc gcgacgaaga agaataatca tccccccacg acgtgttcct gaacgcccgc
     6001 atatgcgggc gttttgcttt ttggcgcgcc ttgttacctg atgcagcgta aaccccttat
     6061 ctggcctacg atctgcgtac gcaatcaaaa tctcctgcca atacaacatt taacactatc
     6121 atattttcca tcattagtgt gatcagctgg ttattttctg ttgtaatagt gtattaatct
     6181 attcaccgca tcaatattaa gaatctctga cagatgtaaa ctttttcgcg cgttatccct
     6241 tacgcgttca tacttttcag gatggtattg gaaggttaat aaatatgaat acaacaacac
     6301 ccatggggat gctgcagcaa cctcgcccat ttttcatgat cttttttgtc gagttatggg
     6361 agcgattcgg ctactacggc gtgcagggcg tactggcggt tttcttcgtt aaacagcttg
     6421 gattctcgca agagcaggct tttgtcactt ttggtgcttt tgctgcgctg gtctatggcc
     6481 tcatttccat tggcggctat gtcggcgacc acctgctggg gaccaaacgc accattgttc
     6541 tcggagcact tgtgctggcg attggctact tcatgaccgg catgtcgcta cttaagcctg
     6601 acctgatttt catcgccctg gggactatcg ctgtcggtaa cggcctgttt aaagctaacc
     6661 cagccagctt gctttcgaag tgctatccgc cgaaagatcc gcggcttgat ggcgcattca
     6721 ccctgttcta tatgtcgatc aatatcggct cgttgatagc gttatcgctg gcccctgtga
     6781 tcgctgatag atacggttat tcagtcacct acaacctgtg cggggcgggg ttaattatcg
     6841 cattactggt ttacatcgcc tgtcgtggaa tggtgaaaga cattggttct gaacccgact
     6901 tcaagccgat gagcttcagc aaactgttgt acgtattact tggcagcgtg gtgatgatct
     6961 tcgtatgtgc atggctgatg cacaacgtag aagtcgccaa tctggtgctg attgttctct
     7021 ccatcgtcgt caccatcatc ttctttcgtc aggcattcaa gctggataaa accgggcgca
     7081 ataaaatgtt tgtcgccttt gtcctgatgc tcgaagcggt ggtgttttac attctctacg
     7141 cccagatgcc aacgtcgctg aacttctttg ccatcaacaa cgtgcatcat gaaattctcg
     7201 gcttttccat aaacccggtc agcttccagg cgcttaaccc gttctgggtg gtactcgcca
     7261 gcccaatact ggcaggcatt tacacgcatc tgggtaacaa aggcaaagac ctctcgatgc
     7321 cgatgaaatt tactctcggc atgtttatgt gctcactggg ctttttgacg gcggcagctg
     7381 cgggaatgtg gtttgcggat gcacaagggc tgacatcgcc atggtttatc gtgctggtgt
     7441 atttattcca gagcctgggt gaattgttta ttagcgccct tggcctggcg atgattgctg
     7501 ccctggtgcc gcagcatttg atgggcttta ttctcgggat gtggttcctg acgcaggctg
     7561 ccgcattctt gctgggcggc tatgtggcaa catttaccgc agtgccggac aacattaccg
     7621 atccgcttga gacgttaccc gtctatacca acgtgtttgg taagattggc ctggttacgc
     7681 tgggcgttgc agtagtgatg ctgttgatgg tgccgtggct gaaacgcatg attgcgacgc
     7741 cggaaagcca ttaattattc ttgcagaaag cagggtagcg ttatcgctac cctgctttac
     7801 ttttacacgg gcgtacctgc atagatatca aagcgatgcc ctttagtcac aaccgcgttt
     7861 ggcgtggcga cattcgctag cggtggcgcg tagtccgggc gctttactac cacgcgtttg
     7921 gtcgccagca agcgtgcagg ctccagcaat ccatcggcat caagatccgg ccccaccagc
     7981 gactgaaaga cacgcatctc ttttttcacc agcgcgcttt tctgcttatg cgggaacatc
     8041 gggtcgagat agaccacctg cgggcgtggg gtaatatcgg ttagcgccgt caggctggag
     8101 gcgtgaatta actgcaaacg ctcctgcaac cagccgccga tttccgcatc cgcataacca
     8161 cgcgccaggc cgtcgtcgag cagcgcggca acaactggat tacgctccag catccgcacg
     8221 cggcaaccga ctgaagccag cacaaaggca tcgcgcccca gtcctgcagt ggcatccacc
     8281 acatccggca aataatcgcc tttaatgccc accgctttcg ccaccgcctc accgcgacca
     8341 ccgccgaatt tgcgtcggtg cgccatcgct ccgccaacaa aatcaacaaa gatgccgcca
     8401 agttttggct catcacgctt gcgcaattcc agatgttccg gcgttaacac cagcgccatc
     8461 aggttgtctt catcgtgctc cagcccccag cgggccgcca gaacagataa ggcgccgtct
     8521 ccggcgcctg tttcatcaat taagcagatt ttcactgaat gatcagccct taatgccgta
     8581 atgctccagc atcgcatcca gctgcggttc acgaccacgg aagcgtttga acagatccat
     8641 cggctcttct gaaccgccac ggctcagaat gttgtcgagg aacgactgcc cggtttcacg
     8701 gttgaaaatg ccctcttcct caaagcgcga gaaagcatct gccgccagta cgtcagccca
     8761 caggtagctg tagtaacctg ccgcataacc accggcgaaa atatggctga aagcgtgcgg
     8821 gaaacggccc caggacggag atggcaccac ggcaaccagt ttcttgattt ctgccagagt
     8881 ttcgaggatt tttgccccct gatccgggcg gaactcggca tgaaggcgga aatcaaacag
     8941 gccgaactcc agctgacgca gaataaacag cgccgcctgg tagttcttcg ccgccagcat
     9001 tttatccagc aactctttcg gcagcggttc gccggtttca tagtgaccag agataaacgc
     9061 cagcgcctcc ggctcccagc accagttttc cataaactga ctcggcagtt cgaccgcatc
     9121 ccacggcaca ccgctgatac cggaaacacc agcggtttcg atgcgggtca gcatatggtg
     9181 caggccgtga ccgaactcgt ggaacagggt gatcacttcg tcgtgagtga acagcgccgg
     9241 tttaccattt accgggcggt tgaagttaca agtcaaatac gcgaccggtt tttgcagaga
     9301 accatcagct ttacgcatct ggcctacgca gtcatccatc cacgccccgc cgcgcttgtt
     9361 ttcacgggca tacagatcga ggtagaagct gccgcgcagt tcgttatttt cgtcatacag
     9421 ttcgaagaaa cgtacatccg gatgccagac atcaacctct ttacgctctt tagcggtgat
     9481 gccgtaaata cgtttcacca cttcaaacag gccgttaacc gatttgtttt ccgggaagta
     9541 cggacgcagc tgctcgtcgc tgatgctgta gaggtgctgt ttctgttttt cgctgtagta
     9601 agcgatatcc cacggctgca actcatcgac accaaattcg gctttagcga aggcacgcag
     9661 ttgctccagc tctttttcgc cttgcggacg cgcgcgtttt gccagatcgg ttaagaaatc
     9721 cagcacctgc tgcgggtttt ctgccatttt agtggcaagg gatttaaagg cgtagttttc
     9781 aaagcccagc agttgcgcca gttcgtgacg cagcgcgagg atctcttcca tcaccttgct
     9841 gttatcccac ttaccggcgt tcgggccttg atcggaggcg cgggtgctgt aagcgcgata
     9901 catctcttcg cgcagagcct ggttgtcgca gtaggtcatt accggcaggt agcttgggat
     9961 atccagcgtc agcaggtagc cttccagctc tttcgcttcg gcctgggctt ttgccgcagc
    10021 cagcgcgctt tctggcatcc ccgccagccc cgcttcgtcg gtaacgagtt tggtccagcc
    10081 catcgtcgca tcgaggacgt tgttgctgta ctggttgccc agttcagaaa gacgggtagc
    10141 aatttcgccg taacgctgct gtttctcttt cggcagacct atgccagaga gttcaaaatc
    10201 acgcagggcg ttatcaaccg ctttcttctg cgccgtgttc agcgtggcgt aatgatcgcc
    10261 atcgcgcagg tcgcggtacg ctttatacag cccttcatgt tgccctaccc aggtgctgta
    10321 ttccgacagc agcggcaggg tttgctcgta ggcttcacgc agttccgggc tatttttcac
    10381 cgagttcagg tggctgaccg gggagaagat acgccccaac acatcgtcca cttccgccaa
    10441 cggctggcag agattttccc aggtgtacgg tgccccttgc gctactacgc gcgccacatt
    10501 ttcgcggcaa tcgttcagcg cctgagtcac ggctggaacg acatgttccg ggagaatttt
    10561 agaaaacgga ggcaattcaa agggagtcag taacggattc gtcattcgcg cagtcctggt
    10621 taaagaggtt aaggaagcgc tcaacaggcg ctttacataa tgtgtgtagc atggggttaa
    10681 gtgtagtgaa tttcaatgag aaacgttacg ctttcgcggc ggcggcctct tttcggtata
    10741 ctgtcctgat acgcttttgt gcgccccgaa tacgggccga tttttactta ccggaacacc
    10801 tttacccatg ctcagttatc gccacagctt tcacgctggc aaccacgccg acgtccttaa
    10861 acataccgtt cagagcctga tcatcgagtc gctgaaagag aaagataaac cgtttctcta
    10921 tctcgatacc cacgcagggg ccgggcgtta tcagttaggc agcgaacatg ccgagcgtac
    10981 cggcgaatat ctcgaaggta tcgcccgtat ctggcagcag gacgatttgc ccgcagaact
    11041 ggaggcgtac atcaatgtgg taaaacactt caaccgtagc ggtcagttgc gttactaccc
    11101 cgggtcgccg ttgattgctc gccagctact gcgcgaacaa gacagcctgc aactgaccga
    11161 actgcacccg agcgattacc cgttgctgcg ttctgaattt cagaaagaca gccgtgcgcg
    11221 cgtcgaaaaa gccgacggtt tccagcagct taaggccaaa cttccgccgg tttcccgccg
    11281 tggtttaatc cttatcgacc cgccgtatga aatgaaaacc gactatcagg cggtggtcag
    11341 cgggatagca gaaggttaca aacgtttcgc cactggtact tacgcactgt ggtatccggt
    11401 ggtgctgcgt cagcaaatta agcgcatgat ccacgatctg gaagcgaccg gtattcgcaa
    11461 aattctgcaa attgaactgg cggtactgcc agacagcgat cgccgtggca tgaccgcttc
    11521 cggcatgatt gtgattaacc cgccgtggaa actggaacag cagatgaata acgtgctgcc
    11581 gtggctgcac agcaaactgg ttccggcagg caccgggcac gccaccgtaa gctggatcgt
    11641 gccggagtaa ttgcagccat tgctggcacc tattacgtct cgcgctacaa tcgcggtaat
    11701 caacgataag gacactctgt catgactaaa cactatgatt acatcgccat cggcggcggc
    11761 agcggcggta tcgcctccat caaccgcgcg gctatgtacg gccagaaatg cgcactgatt
    11821 gaagccaaag agctgggcgg cacctgcgtg aacgtcggct gcgtgccgaa aaaagtgatg
    11881 tggcacgcgg cgcaaatccg tgaagcgatc catatgtacg gccctgatta tggtttcgat
    11941 accaccatca acaagttcaa ctgggaaacg ttgatcgcca gccgtaccgc ctatatcgac
    12001 cgtattcata cttcctatga aaacgtgctc ggtaaaaata acgttgatgt aatcaaaggc
    12061 tttgcccgct tcgttgatgc caaaacgctg gaggtaaacg gcgaaaccat cacggccgat
    12121 catattctga tcgccacagg cggtcgtccg agccacccgg atattccggg cgtggaatac
    12181 ggtattgatt ctgatggctt cttcgccctt cctgctttgc cagagcgcgt ggcggttgtt
    12241 ggcgcgggtt acatcgccgt tgagctggcg ggcgtgatta acggcctcgg cgcgaaaacg
    12301 catctgtttg tgcgtaaaca tgcgccgctg cgcagcttcg acccgatgat ttccgaaacg
    12361 ctggtcgaag tgatgaacgc cgaaggcccg cagctgcaca ccaacgccat cccgaaagcg
    12421 gtagtgaaaa atgccgatgg tagcctgacg ctggagctgg aagatggtcg cagtgaaacg
    12481 gtggattgcc tgatttgggc gattggtcgc gagcctgcca atgacaacat caacctagaa
    12541 gccgctggcg ttaaaactaa cgaaaaaggc tatatcgtcg tcgataaata tcaaaacacc
    12601 aatattgaag gtatttacgc ggtgggcgat aacacgggtg cagtggaact gacaccggta
    12661 gcagttgcag cgggtcgccg tctctctgaa cgcctgttta ataacaagcc ggatgagcat
    12721 ctggattaca gcaacattcc gaccgtggtc ttcagccatc cgccgattgg tactgttggt
    12781 ttaacggaac cgcaggcgcg cgagcagtac ggcgacgatc aggtgaaagt gtataaatcc
    12841 tctttcaccg cgatgtatac cgccgtcacc actcaccgcc agccgtgccg catgaagctg
    12901 gtgtgcgttg ggccagaaga gaagattgtc ggtattcacg gcattggctt tggtatggac
    12961 gaaatgttgc agggcttcgc ggtggcgctg aagatgggcg caaccaaaaa agacttcgac
    13021 aataccgtcg ccattcaccc aacggcggca gaagagttcg tgaccatgcg ctaaatgtta
    13081 aagggctaag agtagtgtgc tcttagccct taattacgtt tccgctatca gttcagaagc
    13141 tgaagcagaa agcggatcag ttccagcagc gcaattaacg ctccaagaac gatgattgct
    13201 ttatcaatca cccgttttct ccatgcgatg gagtgagaat gcatccgctt actcatccac
    13261 tgcctgtcac ggcgcatgtc tcattgttag atgacgacta tctcactccg gccagagcat
    13321 cagttaacgg caccacccgt acttctgacc aggactttga aagcgtttat gcgcattacc
    13381 agagtgaaaa tgcctcagag ctaactggat aatcatacag tacattcagg ttataaaacc
    13441 agcatgtcct tgcaatagtt tcagtatggt attagcattg atgcgttaga tgatgactat
    13501 ctcactccag ccagagccac caactcaggg ctgaagagta aaaaaaccga cgcaaagtcg
    13561 gtttttttac atccggattc ggacaaggct taatatgacg atgacccagt gaaagtatat
    13621 aaatcgtcat tgcgatatat accgaagtgc cccctccgcc agctgaagaa atcgctaatt
    13681 cttgcaatgt tagccactgg ctaatagtat tgaaccgtta gatgtgggca ttctcactcc
    13741 agccagagcc accaactcag gctgaagagt aaaaaaccga cgcaaagtcg gtttttttac
    13801 gtcctgattc aaaccgcctt tcaaatgaat agccaactca aaattcacac ctattacctt
    13861 cctctgcact tacacattcg ttaagtcata tatgtttttg acttatccgc ttcgaagaga
    13921 gacactacct gccacaatca ggagcgcaat atgtcatttc tgttacccat ccaattgttc
    13981 aaaattcttg ctgatgaaac ccgtctgggc atcgttttac tgctcagcga gctgggagag
    14041 ttatgcgtct gcgatctttg tactgccctc gatcagtcgc agcccaagat ctcccgccac
    14101 ctggcattgc ttcgtgaaag cgggctattg ctggaccgca agcaaggtaa gtgggttcat
    14161 taccgcttat caccgcatat tccagcatgg gcggcgaaaa ttattgatga ggcctggcga
    14221 tgtgaacagg aaaaggttca ggcgattgtc cgcaacctgg ctcgacaaaa ctgttccggg
    14281 gacagtaaga acatttgcag ttaaaaattt agctaaacac atatgaattt tcagatgtgt
    14341 tttatccggg aggcattatg ttactggcag gcgctatctt tgtcctgacc atcgtattgg
    14401 ttatctggca gccgaaaggt ttaggcatcg gctggagtgc aacgctcggc gcagtactgg
    14461 cgttagttac gggcgtggtt catccgggtg atattccggt ggtgtggaat atcgtctgga
    14521 acgcgacggc tgcgtttatc gccgtcatta tcatcagcct gctgctggat gagtccggct
    14581 tttttgaatg ggcggcgctg cacgtctcac gctggggtaa tggtcgtggt cgcttgctgt
    14641 ttacctggat tgtcctgctc ggtgctgccg ttgccgccct gtttgccaat gatggcgcgg
    14701 cgcttatttt gacgccgatt gtcatcgcca tgctgctggc tttaggattc agtaaaggca
    14761 cgacgctggc gttcgtgatg gcggccggat tcattgccga taccgccagc ctgccgctta
    14821 ttgtctccaa cctggtgaat atcgtttccg ctgatttctt tggcctcggc tttcgcgaat
    14881 acgcctcggt gatggtgccg gtggatatcg ccgcgattgt tgccacgctg gtgatgctac
    14941 atctctattt tcgcaaagat attccgcaga actacgatat ggcgctgctg aaatctcccg
    15001 cagaagcgat caaagatcct gctacgttca aaactggctg ggttgtttta ctgcttctgc
    15061 tggtgggatt tttcgtcctg gaaccgctcg gcattccggt gagcgccatt gcagctgtgg
    15121 gcgcgctgat attatttgtc gtcgctaaac gcggtcatgc gattaatacg ggtaaagtcc
    15181 tgcgcggtgc cccctggcag attgtcatct tctcgctcgg catgtatctg gtggtttatg
    15241 gcctgcgcaa tgccggatta acggaatatc tttctggcgt actcaacgtg ctggcggata
    15301 acggcctgtg ggccgcgacg ctcggcaccg gattcctcac cgccttcctc tcttctatta
    15361 tgaacaatat gccgacggta ctggttggcg cgttgtccat tgatggcagc acggcatctg
    15421 gcgttatcaa agaagcgatg gtttatgcca atgtgattgg ctgcgatttg ggaccgaaaa
    15481 ttaccccaat tggtagcctg gctacgctac tctggctgca cgtactttcg cagaagaata
    15541 tgactatcag ctggggatat tacttccgta cagggattat catgaccctg cctgtgctgt
    15601 ttgtgacgct ggctgcgctg gcgctacgtc tctctttcac tttgtaatga gatactgata
    15661 tgagcaacat taccatttat cacaacccgg cctgcggcac gtcgcgtaat acgctggaga
    15721 tgatccgcaa cagcggcaca gaaccgacca ttatccatta tctggaaact ccgccaacgc
    15781 gcgatgaact ggtcaaactc attgccgata tggggatttc cgtacgcgcg ctgctgcgta
    15841 aaaacgtcga accgtatgag gagctgggcc ttgcggaaga taaatttact gacgatcggt
    15901 taatcgactt tatgcttcag cacccgattc tgattaatcg cccgattgtg gtgacgccgc
    15961 tgggaactcg cctgtgccgc ccttcagaag tggtgctgga aattctgcca gatgcgcaaa
    16021 aaggcgcatt ctccaaggaa gatggcgaga aagtggttga tgaagcgggt aagcgcctga
    16081 aataaagcgg cgatatccac tgccgatagt tggtaaagtg cgctttgttt atgtcggatg
    16141 cgacgctaac gcgtcttatc cgacctacaa aaccgtgcaa attcaatata tggcatgaaa
    16201 tatgtagagc gtagcgcatc aggcaatttt gctatttacg cagcactcct cgcgtaagaa
    16261 ttcaattatc gcttccagca ttgcgtattg cgaaacacac atcagcgtcc gactctcacg
    16321 gttttgtttg accagtccta ccgataccag tgctgatata tggtggctga gtgtggagcc
    16381 aggtatccca agctgctttt gcaactcacc gaccggcaac ccttgttccc cggctttgac
    16441 aagatgttta aatatgaaca aacgggtcgg gtgacctagc tctttcagcg ctttagcgac
    16501 ttcttccaac tgcatatgac acccctctgt ctggttgata tttcgataat accagaaata
    16561 tcttgatctg aatttcattt cgattattct ggaaatatag atttatccaa tgaggttacg
    16621 tatgagtagt tggcttgcga tgctgcaaga tgccgcagag atgtttgtgt ttctcgccgt
    16681 cgagctttcg ttgctgttta tagtgattag tgccggtgtc agcctgataa gacaaaaggt
    16741 gccagaccat aaaatccagc agatgatggg ggccagaaaa gggagaggct atctcctggc
    16801 tgctctgttg ggagccgtta ccccgttctg tagttgctcg acaataccca tgttacgtgg
    16861 attgttgtca gcgaaagccg ggt
//
LOCUS       DAZSBF010000081        16548 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZSBF010000081 DAZSBF010000000
VERSION     DAZSBF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.082"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            105..299
                     /locus_tag="ACHXWD_002073"
                     /pseudo
     CDS             105..299
                     /locus_tag="ACHXWD_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            522..1019
                     /gene="ubiC"
                     /locus_tag="ACHXWD_002074"
     CDS             522..1019
                     /gene="ubiC"
                     /locus_tag="ACHXWD_002074"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR7620994.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1032..1904
                     /gene="ubiA"
                     /locus_tag="ACHXWD_002075"
     CDS             1032..1904
                     /gene="ubiA"
                     /locus_tag="ACHXWD_002075"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR7620995.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2059..4482)
                     /gene="plsB"
                     /locus_tag="ACHXWD_002076"
     CDS             complement(2059..4482)
                     /gene="plsB"
                     /locus_tag="ACHXWD_002076"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR7620996.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4653..5021
                     /gene="dgkA"
                     /locus_tag="ACHXWD_002077"
     CDS             4653..5021
                     /gene="dgkA"
                     /locus_tag="ACHXWD_002077"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR7620997.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5131..5739
                     /gene="lexA"
                     /locus_tag="ACHXWD_002078"
     CDS             5131..5739
                     /gene="lexA"
                     /locus_tag="ACHXWD_002078"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR7620998.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5812..7137
                     /gene="dinF"
                     /locus_tag="ACHXWD_002079"
     CDS             5812..7137
                     /gene="dinF"
                     /locus_tag="ACHXWD_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR7620999.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7253..7462
                     /locus_tag="ACHXWD_002080"
     CDS             7253..7462
                     /locus_tag="ACHXWD_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR7621000.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7504..8019)
                     /gene="zur"
                     /locus_tag="ACHXWD_002081"
     CDS             complement(7504..8019)
                     /gene="zur"
                     /locus_tag="ACHXWD_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR7621001.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8337..8591
                     /gene="yjbL"
                     /locus_tag="ACHXWD_002082"
     CDS             8337..8591
                     /gene="yjbL"
                     /locus_tag="ACHXWD_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR7621002.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8615..9322
                     /locus_tag="ACHXWD_002083"
     CDS             8615..9322
                     /locus_tag="ACHXWD_002083"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR7621003.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            9685..10722
                     /gene="dusA"
                     /locus_tag="ACHXWD_002084"
     CDS             9685..10722
                     /gene="dusA"
                     /locus_tag="ACHXWD_002084"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR7621004.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            10856..11098
                     /gene="pspG"
                     /locus_tag="ACHXWD_002085"
     CDS             10856..11098
                     /gene="pspG"
                     /locus_tag="ACHXWD_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR7621005.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(11264..12247)
                     /locus_tag="ACHXWD_002086"
     CDS             complement(11264..12247)
                     /locus_tag="ACHXWD_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR7621006.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            12330..13745
                     /gene="dnaB"
                     /locus_tag="ACHXWD_002087"
     CDS             12330..13745
                     /gene="dnaB"
                     /locus_tag="ACHXWD_002087"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR7621007.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13798..14877
                     /gene="alr"
                     /locus_tag="ACHXWD_002088"
     CDS             13798..14877
                     /gene="alr"
                     /locus_tag="ACHXWD_002088"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR7621008.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            15130..16323
                     /gene="tyrB"
                     /locus_tag="ACHXWD_002089"
     CDS             15130..16323
                     /gene="tyrB"
                     /locus_tag="ACHXWD_002089"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR7621009.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         3945 a         3952 c         4368 g         4283 t
ORIGIN      
        1 ccggggtgta gcaacagctc acccatgcgg ctctgacaac gatgttaacc cgcgttaaat
       61 atcccacgtt ggccagcatc tgtctcacgg aaagtcatac tgccttacat gatatgttga
      121 atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga cataagatgt
      181 tgcctattaa tcttactcag gaaacacttt ttagctccaa atctcatggt aaatatgcgc
      241 ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaatata ggtatttaag
      301 taaaggaaaa catcaccgtt cctggcatca tgggcggtga tgcccctacg gttgccctcg
      361 ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt
      421 tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta aacacttttc
      481 tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac cccgcgttaa
      541 cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggattcg caactgctcg
      601 actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga aaaacggtga
      661 gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa gaactgccgc
      721 tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc gatggtgaac
      781 cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt
      841 tacaaaaatt gggtaaaacg ccgctaggac gctatctgtt cacatcatcg acattaaccc
      901 gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat
      961 taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag
     1021 aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt catcgcttaa
     1081 tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta tgggcgttgt
     1141 gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc gcgggtgtct
     1201 ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag tttgatggtc
     1261 atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag aaagaggcgc
     1321 gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg ctgaatacga
     1381 tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg tttatgaagc
     1441 ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg
     1501 cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc ctcgccaata
     1561 ttctctgggc ggtggcttac gacacgcagt atgcaatggt tgaccgtgac gatgatgtga
     1621 agattggtat taaatccacc gcaatcctgt tcggccaata cgataaattg attattggta
     1681 ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat ggcttaggct
     1741 ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt tgtttatcaa caaaaactga
     1801 ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac tatgttggtc
     1861 tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt aaaaaagccg
     1921 gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag gcctacacaa
     1981 cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt tttcgccgca
     2041 tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac
     2101 gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg ccggttcggc
     2161 gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag aactgaacac
     2221 cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac gttgcgcaac
     2281 ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac tcaacaacca
     2341 gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca gctgtagggg
     2401 gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca gcccctgacg
     2461 ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat cccagcgcag
     2521 gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca atacgtcgcg
     2581 ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca ccaacatatg
     2641 cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca gaatgatgat
     2701 gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag
     2761 ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt tgcgcatcag
     2821 atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga
     2881 tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg
     2941 taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg gacgcaccgc
     3001 ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa
     3061 cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc
     3121 gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta
     3181 agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg taatcggacg
     3241 cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt tcggatccag
     3301 caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat aaccacggct
     3361 gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc ctttaaaagt
     3421 acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc agaagttcag
     3481 gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca cgtaagaaag
     3541 tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat gaccgtcgtg
     3601 ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa gtcggttcca
     3661 ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat tcgccgcaat
     3721 ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt ttttgctgcg
     3781 cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat taaacagatc
     3841 ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa agtgcatacg
     3901 cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat ccgccatacg
     3961 gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc acagtacggc
     4021 gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc ctttttcacg
     4081 ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca gatttgggtt
     4141 gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt tcggcgtgta
     4201 ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc
     4261 gatttctagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg cgcgtaacgt
     4321 cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag aggtatccag
     4381 ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga tgcttaatgg
     4441 taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg taaagcctct
     4501 ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc
     4561 cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg gtacactgat
     4621 attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact ggattcaccc
     4681 gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg atcaacgaag
     4741 cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg
     4801 atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg atgattgtgg
     4861 aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa taccatgagc
     4921 tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgcgatt atcgtcgccg
     4981 tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag atttcgataa
     5041 atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta tatactcaca
     5101 gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag gcaacaagag
     5161 gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa
     5221 atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg
     5281 gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag
     5341 gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg
     5401 cagcagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc gaatgctgat
     5461 ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggtgacttg
     5521 ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat
     5581 gacgaagtta ccgttaagcg cctgaaaaaa cagggtaata aagtcgaact gttgccagaa
     5641 aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac cattgaaggg
     5701 ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga gaccgcgatg
     5761 ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg catggcattc
     5821 ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt ctccaatatc
     5881 accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga tagcccggtt
     5941 tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat gctgttgctg
     6001 tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa aaatcctcag
     6061 gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg ggcgttaatt
     6121 gcgctgctgc gtacaccgat tatcgatctg gctctgcata ttgttggcgg tagtgaagca
     6181 gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc ggcgtcgctg
     6241 gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt
     6301 ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctagt gatggggctg
     6361 catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc aacattgctg
     6421 attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg cgaaatgtcg
     6481 aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga tatcatgctg
     6541 cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggcgc gcgactgggg
     6601 agtgacatta tcgctgtaaa cgcggttctg atgacgctac tcacctttac cgcctatgcg
     6661 ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg tgcgcgcgac
     6721 ggtagccagt tgctggatgt ctggcgggca gcgtgccgcc agtcggggat cgtagcgtta
     6781 ctattttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct gacgtcgtta
     6841 acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt gccgttggtt
     6901 ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc cgccgaaatg
     6961 cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac gctgccgtgg
     7021 ctgggtaatc atggtttgtg gctggcacta accgtctttc tggcgttgcg cgggctttct
     7081 ctggcggcta tctggcggcg tcactggcgt aacggtacct ggtttgccgc aacgtgacgg
     7141 ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa tctcacgttc
     7201 agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga ttatgaataa
     7261 agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa
     7321 actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg tcggtaaaat
     7381 ccaggaacgg tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac
     7441 ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag gatgtacgcc
     7501 ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact gttcaggatg
     7561 acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa tcacattatg
     7621 ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca cgccttctgc
     7681 acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg tatgcgtggg
     7741 ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat gcacaaaacc
     7801 ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt gcggttcagc
     7861 ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca gactcatcag
     7921 gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc agattttttc
     7981 agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca aagtgggggt
     8041 taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt attgttgccg
     8101 taatgttgat tttctgtttt gtaggtaagg tgttatgttg ccttgtcgta ccattatcaa
     8161 cacgataata attaataatt catttttaaa taaattgctt caagttaact cgctatttat
     8221 ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt tgatagtgac
     8281 cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga tgaatcatgt
     8341 tgaagataat acccggtgca actggatact ttaataaaac actcaattca aatcaattcg
     8401 ataatgaaga tgcaatcaaa gataaattag ataatagagg ttcaatcaaa ggtaaattaa
     8461 ataatatata tggtaaatct attgattatg cagcgctgcg tcatcgggat ataattattg
     8521 caaaaataga tttgtttatt caacgaatca cgcataattt atggcatgca cgtaaaaaaa
     8581 tgtgttttta attgagcaaa taaatgatct caaaatgtgg gtcaataagt atattgacga
     8641 ttgcactgat gaggatttaa acgatcgtga ctttattgca tcagttgttg accgggctat
     8701 ttttcatttc gcgattaata gtatatgtaa tcctggggat aataaagatg cgactcccat
     8761 tgaacaatgt acttttgatg tagaaactaa gaatggcctt ccctccacgg ttcagctatt
     8821 ttatgaggaa tctaaggata atgaaccttt agcgaatata cattttcaag caataggttc
     8881 tggtttttta acgtttgtta atgcctgcca ggaacatgat gacaacagct taaaattatt
     8941 tgcttcactg ttaatttcac tttcatattc tagtgcctac gcagatttat cagaaacagt
     9001 gtatattaat gaaaataatg agagctacct gaaagctcag tttgaaaaat tatctcaacg
     9061 tgatatgaag aagtacctgg gagagatgaa gcgtctggct gatgggggag aaatgaattt
     9121 tgatggctat ctggataaga tgtcacatct ggtgaatgaa ggaacgctcg atcctgatat
     9181 tttaagcaaa atgcgagatg ctgcaccaca attaattagc ttcgcgaagt cgtttgaccc
     9241 aacctcaaag gaagagatta aaatacttac agacacttct aaattaattt atgatttgtt
     9301 cggggttaaa tcggagaaat aatatgtgaa gttcttcgat agtatggaag gcattatata
     9361 aaaggaccca atatttattg ggttcttttt tctctatcaa tgctattagc agggagatat
     9421 atcaccagag tttaatgtgt gattttttat ttatcgtcga acctggattg tttatcattg
     9481 gccttaacaa agttaacggc taataagatt atttccatca cttcgtgaga gcttcatgcc
     9541 ttgagaggat ctcaattttc ttttgcaatg agacaggcgc ttcctgttgt tatggtatag
     9601 taccccgcta ttgagcctcc tgaatagtga tgctgaataa cataacccca tgatatatcg
     9661 ataaaataat ctctacattt gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa
     9721 accagcgcaa tgcctgaaaa aactgacgtt cactggagtg gtcggtttag cgttgcacca
     9781 atgctcgact ggacggacag acattgccgc tatttcttgc gtctgctttc ccgcaatacg
     9841 ttgctgtata ccgaaatggt gaccacaggg gcgattattc acggtaaagg tgattacctg
     9901 gcgtacagtg aagaagaaca tccggtagcg ttgcaactcg gcggtagcga tccggcggcg
     9961 ctggcacagt gtgcgaagct ggcagaagcg cgtggatatg atgagatcaa cctgaatgtc
    10021 ggctgcccgt ctgaccgggt gcagaacggc atgtttggtg cgtgtctgat gggtaatgcg
    10081 cagctggttg ccgactgcgt gaaagcgatg cgcgatgtgg tgtcgattcc agtgacggtg
    10141 aaaacgcgta ttggcatcga tgaccaggac agctatgaat ttctctgcga tttcatcaac
    10201 accgtttccg gcaaaggcga gtgtgagatg tttatcatcc acgcacgtaa agcctggctt
    10261 tcggggttaa gcccgaaaga aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat
    10321 caactgaagc gtgactttcc gcatctgaca atgtcgatta acggtggtat caagtcgctg
    10381 gaagaggcca aagcacacct gcaacatatg gatggcgtga tggtcgggcg cgaggcgtat
    10441 cagaatccgg gtattctggc ggcggtagac cgggagatct ttggttcctc ggataccgat
    10501 gccgatccgg tggcggtagt gcgcgccatg tatccgtaca ttgagcgtga actcagccag
    10561 gggacgtatc tcggccatat tacccggcat atgttgggct tgttccaggg tattcctggc
    10621 gcgcggcagt ggcggcgtta tttaagtgaa aatgcccata aagcgggtgc agacattaat
    10681 gtgctggaac acgcgctcaa actggtggcg gataagcgtt aacttttcac caaaaagtag
    10741 tcaaattcac cacgccctgc gcaccgtcgc ggggcgtttt gctgttaaat caatagatta
    10801 tttttggcat gattcttgta atgccagcaa gagatttcat atttgggaga gcatcatgct
    10861 ggaactactt tttgtgattg gcttttttgt catgctgatg gtcaccggcg tttcgttgct
    10921 gggcattatc gccgcgctgg ttgtggcgac ggccattatg ttcctcggcg gtatgctggc
    10981 attgatgatt aagttgctgc cgtggttact actggcgatt gcggtggtgt gggttattaa
    11041 ggcgattaaa gcaccaaaag tgccgaaata tcagcgttat gaccgctggc gttactaagg
    11101 gattgtgcgg atgatcacaa cctaaggttt tatccttaga acaaaatagg aattgataat
    11161 caaatctgtc actattgcgc ctctaacaga ttcatcgtgc tgtaccctac atacagccga
    11221 actataaaaa gaaagggctt cccaggtgga agccctattt cttttatgga atcaacaggc
    11281 tggaaccttg cgtcgcccgg ctttccagaa tctcatgcgc acgctgcgca tccttcagcg
    11341 gatatttctg ctgctcggcg acatcgacct taatcacacc gctggcaatc aaagagaaca
    11401 gttcattact ggcctcggtt aattcctccc gcgtggtgat atagccttgc agggaagggc
    11461 gtgtcacata caacgagcct ttttgattga gaatgcctaa gttcacaccg gtaaccgcac
    11521 ctgatgagtt gccaaaactg accattaagc cgcggcgttg caggcaatcc agcgaccgtt
    11581 cccaggtgtc tctgcccacg gaatcgtaca ccacgcgcac tttcttaccg ccggtgatct
    11641 cttttaaccg ctcgaccaga tcctcttcac gatagttaat aacctgccac gcgcccgctt
    11701 ttagcgcgct ctgcgctttt tgcgcggttc ctacggtgcc gataagtttc gcgcccaggg
    11761 cttttgccca ctggcaggca attaagccaa cgccgccagc cgctgcgtgg aacaggaact
    11821 gttcatcggg tttaatttca taggttttgc gcagcagata ataaaccgtt aagcctttca
    11881 ggaaggatgc cgcagcttgc tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg
    11941 cattaatgtt atgcacagag ctgtaagcgc ctaacgccga ctgcgcatag actacacgat
    12001 cgcctgcctt aatatgcttt acaccactgc cgactttact cacgatgcct gccgcttcgg
    12061 tacctaatcc gctgggtagt gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga
    12121 taaaattgat gccgatggct ttattttcga cctggatttc attctctgcc ggatcggcag
    12181 gagtgaactc tacggcttga agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg
    12241 ttgccatgtg tcctccagaa cgtatcgtca gggtctgctt catatggtaa agtttcgacc
    12301 cattctttat ctcggtaact ccattcacta tggcaggaaa taaacccttc aacaaacagc
    12361 aggctgaacc ccgcgaacgc gatccacaag ttgccgggct gaaagtgcct ccgcactcga
    12421 tcgaagcgga gcagtcggtg ttgggcggtt taatgctgga taacgaacgc tgggacgatg
    12481 tagccgagcg tgtggtggca gacgattttt acacccgccc acaccgccat atctttactg
    12541 aaatggcgcg tttgcaggaa agcggtagtc ctatcgatct gattaccctt gcggaatcgc
    12601 tggaacgcca ggggcaactc gatagcgtcg gtggttttgc ttatctggca gagctgtcaa
    12661 aaaatacgcc aagtgcggcg aacatcagtg cttatgctga catcgtgcgt gaacgtgccg
    12721 ttgtccgtga gatgatctcg gttgcgaatg agattgctga agccggtttt gatccgcagg
    12781 ggcgtaccag cgaagatctg ctggaccttg ctgaatcccg cgtctttaaa attgccgaaa
    12841 gtcgtgcaaa caaagacgaa gggccgaaga acatcgccga tgtgctcgac gcaaccgtgg
    12901 cgcgtattga gcagttgttt cagcagccac acgatggcgt taccggagta aacaccggtt
    12961 atgacgatct caacaaaaaa accgctggct tgcagccgtc ggatttgatc atcgtcgccg
    13021 cgcgtccgtc gatgggtaaa acaacatttg cgatgaacct cgtcgaaaac gcggcgatgt
    13081 tgcaggataa accagtactt atcttctcgc tggagatgcc ttcagaacag attatgatgc
    13141 gttctctggc gtcgctgtcg cgcgttgacc agactaaaat ccgtaccggg cagctcgatg
    13201 atgaagactg ggcgcgcatt tccggcacca tgggtatttt gctcgaaaaa cgcaatatct
    13261 atatcgatga ctcctccggc ctgacgccaa cggaagtgcg ttcccgcgca cgccgtattg
    13321 cccgtgaaca cggcggcatc gggcttatca tgatcgacta cctgcaactg atgcgcgtac
    13381 cggcgctttc cgataaccgt acgctggaaa ttgcagaaat ctctcgctcg ctgaaagcac
    13441 tggcgaaaga actgaacgtg ccggtggtgg cgctgtccca gttgaaccgt tctctggaac
    13501 aacgtgccga caaacgcccg gtcaactccg acctgcgtga atctggctct atcgagcagg
    13561 atgcggactt gattatgttt atctatcgtg atgaggtgta tcacgaaaac agtgatttaa
    13621 aaggcatcgc ggaaattatt atcggtaaac aacgtaacgg cccaatcggg acggtacgcc
    13681 tgacctttaa cggtcaatgg tcgcgcttcg acaactatgc ggggccgcag tacgacgacg
    13741 aataataatt attttatgaa ttaggtaatt aaagcaaaca cttatcaagg aacacaaatg
    13801 caagcggcaa ctgttgtgat taaccgccgc gctctgcgac acaacctgca acgtcttcgt
    13861 gaactggccc ctgccagtaa aatggttgcg gtggtgaaag cgaacgctta tggtcacggt
    13921 cttcttgaga ccgcgcgaac gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa
    13981 gaagctctgc gactgcgtgc ggggggaatc accaaacctg tactgttact cgaaggcttt
    14041 tttgatgcca gagatctgcc gacgatttct gcgcaacatt ttcataccgc cgtgcataac
    14101 gaagaacagc tggctgcgct ggaagaggct agcctggacg agccggttac cgtctggatg
    14161 aaactcgata ccggtatgca ccgtctgggc gtaaggccgg aacaggctga ggcgttttat
    14221 catcgcctga cacagtgcaa aaacgttcgt cagccggtga atatcgtcag ccattttgcg
    14281 cgcgcggatg aaccaaaatg tggcgcaacc gagaaacaac tcgctatctt taataccttt
    14341 tgcgaaggca aacctggtca acgttccatt gccgcgtcgg gtggcattct gctgtggcca
    14401 caatcgcatt ttgactgggt gcgcccgggc atcattcttt atggcgtctc gccgctggaa
    14461 gatcgctcca tcggtgccga ttttggctgt cagccagtga tgtcactaac ctccagcctg
    14521 attgccgtgc gtgagcataa agccggagag cctgttggtt atggtggaac ctgggtaagc
    14581 gaacgtgata cccgtcttgg cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc
    14641 gctccatccg gtacgccagt gctggtgaac ggtcgcgaag taccgattgt cggacgcgtg
    14701 gcgatggata tgatctgcgt agacttaggt ccacaggcgc aggacaaagc cggggatccg
    14761 gtcattttat ggggcgaagg tttgcccgta gaacgtatcg ctgaaatgac aaaagtgagc
    14821 gcttacgaac ttattacgcg cctgacttca agggtcgcga tgaaatacgt ggattaatcg
    14881 ttctgtaata tttgattgtc tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa
    14941 atcctaaaaa ttcaataagt tgatgttctt tcatgctctt ataaaggtcg tgcctctggc
    15001 ggatgttcgt ttgtcatgag tctcactctg ttgctaattg ccgttcgctc ctgaacatcc
    15061 actcgatctt cgccttcttc cggtttattg tgttttaacc acctgcccgt aaacctggag
    15121 aaccatcgcg tgtttcaaaa agttgacgcc tacgctggcg acccgattct tacgcttatg
    15181 gagcgtttta aagaagaccc tcgcagcgac aaagtgaatt taagtatcgg tctgtactac
    15241 aacgaagacg gaattattcc acaactgaaa gccgtggcgg aggcggaagc gcgcctgaat
    15301 gcgcagcccc atggcgcttc gctttattta ccgatggaag ggcttaacag ctatcgccat
    15361 gccattgcgc cgctgctgtt tggtgcggac catccggtac tgcaacaaca gcgcgtagca
    15421 accattcaaa cccttggcgg ctcaggggca ttgaaagtgg gcgcggattt cctgaaacgc
    15481 tacttcccgg aatcaggcgt ctgggtcagc gatcctacct gggaaaacca cgtagcaata
    15541 ttcgccgggg ctggattcga agtgagtact tacccctggt atgacgaagc gactaacagc
    15601 gtgcgcttta atgacctgtt ggcgacgctg aaaacattac ctgcccgcag tattgtgttg
    15661 ctgcatccat gttgccacaa cccaacgggt gccgatctca ctaatgatca gtgggatgcg
    15721 gtgattgaaa ttctcaaagc ccgcgagctt atcccattcc tcgatattgc ctatcaagga
    15781 tttagtgccg gtatggaaga ggatgcctac gctattcgcg ccattgccag cgctggatta
    15841 cccgctctgg tgagcaattc gttctcgaaa attttctccc tttacggcga gcgcgtcggc
    15901 ggactttctg ttctgtgtga agatgccgaa gccgctggcc gcgtactggg gcaattgaaa
    15961 gcaacagttc gccgcaacta ctccagcccg ccgaattttg gtgcgcaggt ggtggctgca
    16021 gtgctgaatg acgaggcatt gaaagccagc tggctggcgg aagtagaaga gatgcgtact
    16081 cgcattctgg caatgcgtca ggaattggtg aaggtattaa gcacagagat gccagaacgc
    16141 aatttcgatt atctgcttaa tcagcgcggc atgttcagtt ataccggttt aagtgccgct
    16201 caggttgacc gactacgtga agaatttggt gtctatctca tcgccagcgg tcgcatgtgt
    16261 gtcgccgggt taaatacggc aaatgtacaa cgtgtggcaa aggcgtttgc tgcggtgatg
    16321 taatgcagga aagcaggctg gagttaccca gcctgcagtg aaattaaact gtcgtcgctt
    16381 tcactatttc tttgtagatg atttttttga tgccgttgtt ctacgtgaga gataataaaa
    16441 cgttgtcagt tcttttattg ttaagtttat cccaattatc tggaattcct ttccctattt
    16501 tttcgtgtgg agtaataatt tctgcacatc taaccattat taaattta
//
LOCUS       DAZSBF010000082        16513 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZSBF010000082 DAZSBF010000000
VERSION     DAZSBF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.083"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            390..890
                     /gene="tssB"
                     /locus_tag="ACHXWD_002090"
     CDS             390..890
                     /gene="tssB"
                     /locus_tag="ACHXWD_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR7621010.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            925..1149
                     /locus_tag="ACHXWD_002091"
     CDS             925..1149
                     /locus_tag="ACHXWD_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621011.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1200..2675
                     /gene="tssC"
                     /locus_tag="ACHXWD_002092"
                     /pseudo
     CDS             1200..2675
                     /gene="tssC"
                     /locus_tag="ACHXWD_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2682..3095
                     /gene="tssE"
                     /locus_tag="ACHXWD_002093"
     CDS             2682..3095
                     /gene="tssE"
                     /locus_tag="ACHXWD_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR7621012.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3099..4949
                     /gene="tssF"
                     /locus_tag="ACHXWD_002094"
     CDS             3099..4949
                     /gene="tssF"
                     /locus_tag="ACHXWD_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR7621013.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4913..5995
                     /gene="tssG"
                     /locus_tag="ACHXWD_002095"
     CDS             4913..5995
                     /gene="tssG"
                     /locus_tag="ACHXWD_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR7621014.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            6020..7300
                     /gene="tagH"
                     /locus_tag="ACHXWD_002096"
     CDS             6020..7300
                     /gene="tagH"
                     /locus_tag="ACHXWD_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR7621015.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7297..7821
                     /gene="tssJ"
                     /locus_tag="ACHXWD_002097"
     CDS             7297..7821
                     /gene="tssJ"
                     /locus_tag="ACHXWD_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR7621016.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7824..9153
                     /gene="tssK"
                     /locus_tag="ACHXWD_002098"
                     /pseudo
     CDS             7824..9153
                     /gene="tssK"
                     /locus_tag="ACHXWD_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            9158..9919
                     /gene="icmH"
                     /locus_tag="ACHXWD_002099"
     CDS             9158..9919
                     /gene="icmH"
                     /locus_tag="ACHXWD_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR7621017.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9928..12711
                     /gene="tssH"
                     /locus_tag="ACHXWD_002100"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9928..12711
                     /gene="tssH"
                     /locus_tag="ACHXWD_002100"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12708..13451
                     /gene="vasI"
                     /locus_tag="ACHXWD_002101"
     CDS             12708..13451
                     /gene="vasI"
                     /locus_tag="ACHXWD_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR7621018.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13456..14868
                     /gene="tssA"
                     /locus_tag="ACHXWD_002102"
     CDS             13456..14868
                     /gene="tssA"
                     /locus_tag="ACHXWD_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR7621019.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14887..>16513
                     /gene="tssM"
                     /locus_tag="ACHXWD_002103"
     CDS             14887..>16513
                     /gene="tssM"
                     /locus_tag="ACHXWD_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFR7621020.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         3812 a         4556 c         4557 g         3588 t
ORIGIN      
        1 ttgataattt ttgagatatt ccttaaaaat acccacttaa tcaatcacaa aacaaatata
       61 taaacaagca atcattagat tagcttattt ttaatgaaat tattatatac ttcaataagt
      121 tacaaaaaaa ctctatattt aaattattgc cataatacac ctgaagcgaa ttgcacaggt
      181 tcacacaatc tatttaacgc ttgcagaata cccctcttta cgatcgataa aaagtccttt
      241 tcttatttat tttgaagcgt aaaatgcggg gtgatgaagg agtggtgatt ttattttcac
      301 cgccattagg gcggcattgc cgatattttt ttgcggtaca aatatgccgt gttcatggcc
      361 tgtacgagat aacatgacgg atcactttta tgagcaaaaa atttgaaggc agcgtcgcgc
      421 cccgcgagcg tattaatata tcgtatgtcc caaaaacaga tggccagacg gctgaagtgg
      481 aacttccgct taacatgctc gttgtcggcg ataccggaaa tacgcaggaa acatccccac
      541 tggatgaacg tcaggcagtt tccgttaaca agcacaattt cggtgcagtg atggctgaag
      601 cagccattgg cctcaatttt actgtccccg ccacgctgaa gggaagcaca acagacgatg
      661 aactgaatgt ggcgctgaat atcaaatcac tggatgattt ttcaccagat agcgtggccc
      721 ggcaggttcc ggaagtgaat aaattgctgg aactgcgtga agcactgact gcactgaagg
      781 ggccgatggg taaccttccg gcattccgta cccagctcca ggcattgctg gaaaatgaag
      841 agtcccgcga gcagttgctc aaagaaatcg gtctggtgag caataaataa ccaccactat
      901 ttcattaaac agggttatcc aggcatgacg gcacacaaaa atatctccgg gacataccac
      961 ctctctgttg cagacattct gcaggtggtt taccaggtat gtttcagccc gtccgttgaa
     1021 atcaaccagg atggtgtggc tgcgctgatt acaacactgg atcgccgtat cagtgatcta
     1081 ttggatgaga ttatccattt ttgtgagttt cagcagagcg cgtcgcactg gcagcgcgta
     1141 cttcactgat ggcgaaaagc cggtttcaga aacacagcta aaacagaagg attttcagta
     1201 tgtctttaca ggaagaggaa ctcgtttcca gtcatgccgg gcagcctgag caggcatcgt
     1261 cgctgcttga tcaaatcatg gcacagaccc gtattcagcc cggttcggaa ggttatgacg
     1321 ttgccctcca gggtgtgaca gcctttattg ccagcatcct gcaatccact gcatctgccg
     1381 aaccggtcaa taaactggcc gtggacagca tgattgcaga tattgatgaa cgcatcagcc
     1441 gccagatgga tgtcatcatc catgcgcctg cctttcagca ggtcgagtcg ttctggcgct
     1501 cactgaaaac catggtggat cgcgttgatt tccgtgaaaa catcaaggtc aacgtgcttc
     1561 atgtcaccaa gcaggaactt ctggaagact ttgagtttgc gccagaaatt attcagtccg
     1621 gattttataa gcacgtttat tcgtccggtt tcggacagtt tggcggtaaa cccatcgctg
     1681 ccgtgctggg cgcttatgag ttcaaaaata ccgcgcctga catgaaactg ctgcaatacg
     1741 tcagtgccgt gggcgcaatg gcgcacgcac cgttcctgtc ctccgtttcc ccggagttta
     1801 tggggctgaa ctcgtggacc gaactgccta atatcaaaga tctttatgcc atctttgaag
     1861 gtccggccta caccaaatgg cgcgccctgc gtgactcgga agattcgcgc tatttagggc
     1921 ttacggcacc ccgcttcctg cttcgccagc cctattcacc aacagataac cctgttaaga
     1981 actttaacta ttacgaagat gtcagccaga accacgaaga ttatctgtgg ggtaatacgg
     2041 cctggatgct ggcatgcaat atcgcagaca gttttgccaa ataccgctga tgtccaaaca
     2101 ttatcggtcc gcaaagcggc ggcgcagtga aagatctgcc gatgcatctg ttcgaaacga
     2161 tggggcagat tcaggccaaa atcccaaccg aggtgctggt caccgatcgc cgcgaatttg
     2221 aactggctga agagggtttt atcaccctta ccatgcgtaa agactctgat aatgcagcct
     2281 ttttctctgc aaactcggta caaaaaccga agcactttcc gggaaaagat gcagaaacca
     2341 actataagct gggtacgcag cttccttatc tcttcatcat caatcggtta gcgcactaca
     2401 tcaaggtgtt gcagcgtgaa caactggggt catggaaaga aaggagcgac ttagaaagag
     2461 agcttaatac ctggatccgt cagtatgttg ccgaccagga aaacccgcct gctgacgtgc
     2521 gcagccgcaa acctctgcgc gctgccaaag ttgaggttat ggatgtagaa ggcgaacccg
     2581 gctggtacca ggttgcgcta agcgtgagac ctcattttaa atttatgggg gcaaattttg
     2641 agctttccct ggttggccgg ttagacaggg agtaacacac catgataccg gatcgggaaa
     2701 gaaccacagg ttctcttttt gagcgtatgg aagcctcttc ggccagaaac cggcagggag
     2761 gcagtattca ttcgctcagg caatccattc gccagaacct acgaaacatt cttaataccc
     2821 ggtccggtag ttgccggggt gcgcctgagc tgggcattga tgaaccggaa ggggctgaaa
     2881 acttccgcga atccatgagt cgggccatcg agcaatgtat agaacgctat gaaccccgga
     2941 tctcacacgc tgaagtgcag gcagtggttt catccgcctc cagcccgctg gatatgacgt
     3001 ttcatattac agcctgggtg acatttaacg aaacgcatga ggtgcttgaa tttgatatgg
     3061 caccgaatgg cagccagcat taccgggtgg actgacaaat ggaatttgaa gaacgctatt
     3121 tccgggaaga actcgattac ctgcgccagc ttagcaagct gctggcaacg gaaaaacccc
     3181 atctggcccg cttcctggcc gaaaaagatg cagatccgga tattgaacgc ctgctggaag
     3241 gggtggcttt tcttaccggc aatctccgcc agaaaattga ggatgaattc ccagaactga
     3301 cgcacgggct tattaagatg ctatggccta attacctgcg tccggttccg gcaatgaccc
     3361 ttattgaata tacgccggat atggataagt cttctgtacc ggtgttaatc ccccgtaatg
     3421 agcagtttac aaccaacgcc ggggaaatca gagttgatga agtgctgccc tctgatgcta
     3481 aaaaggagga gccgcctccc tgtaccttca ccctctgccg ggatatctgg ctgctgcccg
     3541 ttcgcctgga gcagattgaa aaccgcagta cgacccgtaa tggtgttatc aacatcacct
     3601 tttcggtcgc accgggaacg gacttccgca cgctggatct gaacaaactt cgcttctggc
     3661 tcggcaatga cgacaactat acccgtgacc agctttattt atggttctgc gaatacttgc
     3721 agggtgccga cctgactgtg ggtgaacagc atattcgcct gcctgagttt atgctaaaag
     3781 ctgtcggttt tgagccgcag gatgccatgc tgccctggcc gaaaaacgtc cacagcggct
     3841 accggatcct tcaggagtat ttctgttacc ccgatgcgtt tctctttttt gatctttgtg
     3901 gttgtccggc tttgcctgac ggattgcagg cggaattctt taccctgcaa ctgcgttttt
     3961 cgcgcccttt gcccgtggac atccggctgc gccgcgattc cctgcgcctg tattgcgcac
     4021 ctgccattaa tttatttatc caccatgcag aagccatcac gctggacaac cggcgggcag
     4081 actatccgct ggttcccagc cgccattacc cacaacatta cgatgtattt tccgttaaca
     4141 gtgtggtgag ccaggtccag gatatgttca ggaaaaaaga tctggggcgt cctgtttcga
     4201 cgcaggccgc gcgccagtgg ccagcctttg aaagtttcag ccatcagata gaatacagcc
     4261 ggaagcggga agtggtgtac tggcatcacc ggaccaaaac atccctgttc catcgcggct
     4321 ttgatcatac ccttgccttt atacatgctg atggcagtta tccgtcagac gaatctctgc
     4381 tcagtaatga agtggtttcg gtatcgctga cctgtaccaa ccgtgagctt ccgtcacaaa
     4441 ttcgttccgg cgatatcacc ggcacaaccg gaaaaaatgc ggctgttgct tcatttcgca
     4501 acattacccg cccgacgcaa ccactctggc cggtcattga tggcagcctg cactggcccc
     4561 tactctccgc catgaacctg aattatctgt cattactgga tacggacgcg ctgaagcagg
     4621 tcatcgccaa ctttgatcgc cacgcaatcc atcatccgca gacggcacgg ctgtcacaac
     4681 aaaagctgga tgccattgag cgtctggaga cccgccccgt tgatcgcctg tttacgggta
     4741 ttcccgtccg ggggctggct tccacgctgt atctgcaccc ggagccgttt gtctgtgaag
     4801 gggaaatgta tctgctcggt acggtgcttt cgcattttct gtcgctgtac gccagcgtta
     4861 actcattcca catgctgacc gttgtgaaca cagaaagcca ggagacatgg aaatggacgg
     4921 aaagaatcgg gcagcatcct cttatctgag tccgggaaat cccccggcag ataaagagca
     4981 gaatgatccg ctggcacagg ttttccataa tgcctgctct tacaattttt ttgcgatggc
     5041 ggagctgttg caccgcctgg caaagggtga aaaggggacg ccagaattat ccctgcgcga
     5101 cgatccggca caggaaaccc tgcgtttcag cgccgatgcc agccttgcct tcccgtgcag
     5161 tgatatcagt gcgctgaaac gggatacgtc aggggcattc aggatgacca ccacctttat
     5221 ggggttacag gggagccagt cgcctctccc cggttattac ctcgctcacc tggcctggaa
     5281 agcggtacat gaacaaagtc cggtaggtga ttttctggat atgttcagcc accgcctgac
     5341 ccagtttgtc tggcatatct ggcgtaagta ccgctatcac atcagttttc gtaacggcgg
     5401 tgtggatgcg ttctcgcagc gcatgtactc ccttgtcggg ctgggccacc gccagcttcg
     5461 cgataagctg gcgattaatc acagcaaaat gctggcgtat tccgggatcc tggcaaatcc
     5521 gggacgatcc ccggaaatta tctgtggcct ggtgtcccac tgctttgatt tgtcagaagt
     5581 gacactgcaa aactggcaac gtcgcaaggt ggatattgaa ccggatcagc aaaatagtct
     5641 ggggagctat tctcttaaaa atggcgaaaa actggcggga agatcggtac tgggcaactt
     5701 tgttctgggt acacgagtgc ccgatctcag cggaaaattc cagctcagta ttaccagcct
     5761 gacccgaaaa cagttcctct cttttctgcc gtccggcgaa aacttcctgc cactgacgat
     5821 gttcgtgtcc ttcatcctgc gcgatcagct tgcctgggac ttacatctgg ggctggcgcc
     5881 ggagcaggtg ggcgcaatgc gcctgggcga taacaaaagt gcgctgctgg gctggaccag
     5941 cttcctcggc acaccggaag aacgaccctc agtcaccatc agggtacggt cgtaaattat
     6001 gataatcacg gagcagaata tgccggaaga gaaactacag acgctttcat tgcaggtcat
     6061 taacggcagt gagctggaaa gcgggcgggc ggcgcgctgt ctgttcacac agcagggaaa
     6121 tgtcggccat ggccccgaat gccactggtc ggtacaggat cgtcagcaga gcattccggc
     6181 ccaggctttt accgttatcc tgcacgatgg cacattttgt ctgcgcccgc agacggcaca
     6241 actgtggctg aatcaggcaa aagtcacagc aacatcagac ctaatacagt tgcgccaggg
     6301 cgatgagatc cagatcggac ggctgatggt gagggttcat ctgaaccggg gagatattcc
     6361 ccattacgat gaggaaatgg ccactcccga aaccatcgtt accaatcgcg atatgctcac
     6421 ggataccctg ctatcaacgg agggtgcgcc acactatccg ggaatgactc accggcacca
     6481 gcttgcagac accgtggtaa acggtttttc tgccgatcca ctccaggctc ttcagtccga
     6541 aagcctgatt accacgggcg atccgctttc aggcattgcg gctgtccggc catcggcacc
     6601 gctgtccgat ccggcaagta atggggggat caatactccg tttatggatc tgccgcccat
     6661 ttatgccagc cccggcgatc gtaatgacga cgtttctgcg gcagaaatgg cacaacgcca
     6721 cgttgcggtc acccccttac tgcgcggtct tggcggctcg cttaccatga gcaattccga
     6781 cgatgcggat gattttctgg aggaggccgg acgaacgtta caggccgcaa taaaaggtct
     6841 gctcgatttg cagcagcagc gtaacagcct ctcagacaaa catttgcgcc cgctggaaga
     6901 taacccgctg cgcctgaaca tggattacgc caccgcgctc gacgtaatgt ttgccgaagg
     6961 taaaagcccg gtacatctgg cggctcccgc cgccgtcagt gaaagcctgc gcaatgtccg
     7021 ccaccacgaa gaagctaacc gggcagcgat tgtggagtcg cttcgtgtcc tgctggatgc
     7081 tttctcacca caaaatctgc tgcgccgctt tgtgcagtac cgccgcagcc atgaactgcg
     7141 ccagccgctg gatgatgccg gagcatggca aatgtacagc cattattacg aagaactggc
     7201 ctccgatcgc cagcaggggt ttgagatgct gtttaacgag gtctacgccc aggtctatga
     7261 ccgggtgctt cgtgaaaaac agcgggagcc ggaagcatga acaggaaggc gtttctggcc
     7321 tgcgtgctga tgtgcgtcat attaaccggc tgcgaaacgg cgaaaaaaat cagtgaggtg
     7381 atcaaaaacc cggatatcca ggtcgggagc ctgaaggagc aaccttcgga aataaccgtc
     7441 accctgctga ccgaaccgga caccaatacc aatgcggaag gtgaatcagc cgccgtggat
     7501 gtgcaactgg tgtacctgac tgacgactca aaattgctgg cagccgacta tgaccagata
     7561 gccagcacgc cgctaccgga tgtgctgggt aaaaactata tcgaccacca ggatttcaac
     7621 ctgttgccgg acaccatcaa aaccctgccg ccggtaaagc tggatgagaa aacgcagttt
     7681 attggtgtcg tggcctattt ctccgatgac caggccactg aatggaaaca aatcgagacg
     7741 gtggaaggaa tcggccacca ttaccgcctg ctggtgcatg tacgtcaaag cagcatcgaa
     7801 atgaaaaaag aggatgaata attatggcaa ccacccgcaa caaggtgatg tggcaggaag
     7861 ggatgctgat gcgtccacac catttccagc agcagcagcg ttacaacgac tacctggata
     7921 accagcgttt ccgggccatg aatgatttat cctggggatt taccgaactc accctcaaca
     7981 atgaactgct ggcgcagggt aagatcatga ttgacagcgc gtcaggcaca ctgcccgacg
     8041 gcaccgtctt ttctatcccc gaccaggacg cactgcccga tccgctgcac ccgcaaaatt
     8101 ttccggacga aagaagccgc aatatctacc tcgctctgcc ggtcgccagt gatgtgagaa
     8161 atgaaatcag cgacggacgg cgaatcgggc gttaccggct gaattatgcc gatgtccggg
     8221 atttgcattc agaagaaggt gacgcgcgaa cattgacgct gggtcaactg acgccgcgca
     8281 ttatgagcgg tgcagaagat atgagcgcct atattacgct cccgctttgc cgtatcagcg
     8341 atcgccgccg atggctccct gacgttggat gacgatttta tcccctcctg ccagaatatt
     8401 caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat tggcggacgg
     8461 gcaagcgatc tggcaaaccg tattggctct ccggcgcaga gcggcatcgc ggatgtggcg
     8521 gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca tcacgcccgt
     8581 cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct gggtgaactg
     8641 atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga tcatcatgac
     8701 ctgaccaaaa tatttaaaac gctgttaccg gaagtcaaac gggcgctgca taccgtactg
     8761 tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca ggccgatatc
     8821 catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc aaacatgccg
     8881 gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc
     8941 cgcaatatgg tcagtgtgca gatccccggt attccattgc gtgcccttat ggtggctccc
     9001 cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaagag tggccaggcc
     9061 tggacagaaa tggctgccgc cggggccgtt gcactgcatg tttccggcag tttcccggat
     9121 ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct
     9181 gccaccattt accggacacg acgaaaaaag ccagcgcaac taccatctcg cgcttcgtgg
     9241 caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg taatgcgcct
     9301 ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacagatgt
     9361 tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga ttatctcgtt
     9421 tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcaaacaa
     9481 gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggcga
     9541 gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct
     9601 ggaatttctc tggatctgtt tttctctggg atttcgcggg cgctacaaag tggcagcaca
     9661 ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg
     9721 cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg ggcgttatca
     9781 gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt
     9841 gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct
     9901 aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa
     9961 cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg tatgagtcag
    10021 caggcagcgg aaattaccgt cagccatgtg ctcattcaga tgctcgccat gccacgcagt
    10081 gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgctg
    10141 acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg
    10201 ctggttgagt ggcttaaaga gggctagctg ctggcgtcgg ctgagatgca gcacagcgaa
    10261 ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta tataccgcct
    10321 gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag
    10381 tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca
    10441 atgacggacg cctctgacac cctgcttgcc cgctatgcca aaaacatgac cgcagacgcc
    10501 cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct gatgatcgac
    10561 attctctgcc gccgccgtaa aaacaaccca gtggtggtgg gcgaagcggg cgtgggcaaa
    10621 agcgcactga ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaagctg
    10681 aaaaacaccg atatcatgac ccttgacttg ggcgcattgc aggccggggc gtcggtgaag
    10741 ggtgaattcg aaaaacgttt caaagggctg atggcggagg tcatttcctc cccggtgccg
    10801 gtcattctgt ttatcgacga agcacatacc ctgattggcg cgggcaacca gcagggcggg
    10861 ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa aaccatcgcc
    10921 gccaccacct ggagcgagta caaaaaatac ttcgaaaaag atgccgccct gtcgcgccgc
    10981 ttccagttgg tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgcggt
    11041 ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc cttgcaggcc
    11101 gctgcgacat taagcgagcg ttatctctcc gggcgtcagt taccggacaa agcgattgat
    11161 gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc
    11221 tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag
    11281 cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat
    11341 gaaacgctga cggcgctgga tgaactggaa gcggcctggc accagcagca gacgctggtc
    11401 cgggagatta tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg
    11461 ttgccggacg cagataccgt ggaggatacg cagccagagt cagagtcaga gtcagagtca
    11521 gagtcagagc aggataatac cggtgccgaa ccggctgatg aggccgacag agaacaaccg
    11581 gaagagaccg ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg
    11641 gacgccctgc ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg
    11701 gcggtgattg ccgaatggac cggcgtgccg ctcaaccgcc tgtcgcagaa tgaaatgtcg
    11761 gtcatcaccg acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt
    11821 gccagcctgc ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg
    11881 ctcggtgcgt tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa
    11941 ctggcagagc tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc
    12001 caggagaaac acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt
    12061 gaaggcggcg tactgaccga agcgattcgc cagaaaccct actcggtagt actgctcgat
    12121 gaagtggaaa aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc
    12181 gaaatggcag acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg
    12241 tccaacctcg gctaccaggt aatagtcgag catgccgatg acccggaaac catgcaggaa
    12301 gtacagtatc cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg
    12361 gtgccgtatc tgccgctgtc gaaagagacg ctcgccacca ttatcgccgg aaaactggcc
    12421 cgtctggata acgtgctgcg cagtcgcttt ggtgtagaag tggtcattga accggaagtg
    12481 acggacgaaa tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg
    12541 gtcatcgatg gcgacatgct accgccgctc tcgctgctgc tgttgcagaa aatggcggct
    12601 aacacggcga ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg
    12661 gaagatgctc agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc
    12721 gcgttttccg gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg
    12781 ccgcgcccgc agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc
    12841 cgccggatgc gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc
    12901 tggagcggct tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg
    12961 gggcgctggt caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa
    13021 aacgccgcga gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga
    13081 cggtggtgat aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca
    13141 gttgcgtgga caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg
    13201 atatcgccgt cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg
    13261 agaacggcac cctgctggag tccagccggg ggttatccgg gattgatgaa attaagcaac
    13321 tgttcggcgc aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga
    13381 cctttaacat tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg
    13441 cgggagaata aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg
    13501 ctggaaaacc tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg
    13561 gagtatatcg acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc
    13621 ccggaactcc agcgccgggg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg
    13681 ctggcgcatc tgctaagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg
    13741 ctggcgctgt atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa
    13801 aaacgctttg ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag
    13861 aacgccgcca cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc
    13921 tggcagtcac ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac
    13981 cagcgtgcgt ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt
    14041 tcaccacaga ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc
    14101 caaatcgtca tcgacagtca cgacgacaaa gcctggcgcg acacgctgtt aaaagtggcg
    14161 gccattttat gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg
    14221 ctttggcaga acatcaccag tacgccccag gcggaaagcg atagccgtac tccactggct
    14281 gcggtctccg ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg
    14341 ctgtggcagc aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc
    14401 ctttctgcac agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccacgat
    14461 gaggtcatcc gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac
    14521 acaccgttta tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt
    14581 gcaccggttg cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag
    14641 ggtctggagg cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag
    14701 tttcaccgcc agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg
    14761 cagcagcagt accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag
    14821 ccgtccctgc ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga
    14881 gtttttgtgt tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga
    14941 ccggcgatgc cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt
    15001 ctgctggtgt ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc
    15061 aaaccactgg cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg
    15121 cttaccgtcc gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc
    15181 gaggaagccg ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc
    15241 tggctgctgc gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg
    15301 tatatggtca tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg
    15361 tccgacatta tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg
    15421 ccccatgtcg gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct
    15481 gatgcggata tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaagaaaag
    15541 cgcgcgcgcc agccgctgaa cgggattatt ctgacactcg atttacccga tctgctgacc
    15601 gcagacaaac gccgccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata
    15661 cgccagcatc ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta
    15721 cagggttttg ccgccctgtt ccagtcactc aacagacagg atcgcgatgc gattctgggc
    15781 gtcacgttca cccgccgtgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc
    15841 tggcagacat gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact
    15901 cacacccgcg cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg
    15961 gtgtcactgc ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc
    16021 tatctcacct cttcgcttca gcgtggacag atggatgata tattcaccca gtctgccgcc
    16081 cgccagtacc ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct
    16141 tatttcaccc gcagcctgtt cccgcaggca ttactcgcag agcctaatct ggcaacagag
    16201 agccgcgcct ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc
    16261 gtggcagcac tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc
    16321 ggcatcaccg tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa
    16381 gatgactttg gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc
    16441 tatggcgact ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt
    16501 atcgggcctt atg
//
LOCUS       DAZSBF010000083        16425 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZSBF010000083 DAZSBF010000000
VERSION     DAZSBF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.084"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            66..1712
                     /gene="mqo"
                     /locus_tag="ACHXWD_002104"
     CDS             66..1712
                     /gene="mqo"
                     /locus_tag="ACHXWD_002104"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR7621021.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2021..2530)
                     /gene="eco"
                     /locus_tag="ACHXWD_002105"
     CDS             complement(2021..2530)
                     /gene="eco"
                     /locus_tag="ACHXWD_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR7621022.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2644..2808)
                     /gene="yojO"
                     /locus_tag="ACHXWD_002106"
     CDS             complement(2644..2808)
                     /gene="yojO"
                     /locus_tag="ACHXWD_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR7621023.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2917..3411
                     /gene="napF"
                     /locus_tag="ACHXWD_002107"
     CDS             2917..3411
                     /gene="napF"
                     /locus_tag="ACHXWD_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR7621024.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3401..3664
                     /gene="napD"
                     /locus_tag="ACHXWD_002108"
     CDS             3401..3664
                     /gene="napD"
                     /locus_tag="ACHXWD_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR7621025.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3661..6147
                     /gene="napA"
                     /locus_tag="ACHXWD_002109"
     CDS             3661..6147
                     /gene="napA"
                     /locus_tag="ACHXWD_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR7621026.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            6154..6849
                     /gene="napG"
                     /locus_tag="ACHXWD_002110"
     CDS             6154..6849
                     /gene="napG"
                     /locus_tag="ACHXWD_002110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR7621027.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6836..7699
                     /gene="napH"
                     /locus_tag="ACHXWD_002111"
     CDS             6836..7699
                     /gene="napH"
                     /locus_tag="ACHXWD_002111"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR7621028.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7696..8145
                     /gene="napB"
                     /locus_tag="ACHXWD_002112"
     CDS             7696..8145
                     /gene="napB"
                     /locus_tag="ACHXWD_002112"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR7621029.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            8155..8757
                     /gene="napC"
                     /locus_tag="ACHXWD_002113"
     CDS             8155..8757
                     /gene="napC"
                     /locus_tag="ACHXWD_002113"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR7621030.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8770..9393
                     /gene="ccmA"
                     /locus_tag="ACHXWD_002114"
     CDS             8770..9393
                     /gene="ccmA"
                     /locus_tag="ACHXWD_002114"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR7621031.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9390..10052
                     /gene="ccmB"
                     /locus_tag="ACHXWD_002115"
     CDS             9390..10052
                     /gene="ccmB"
                     /locus_tag="ACHXWD_002115"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR7621032.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            10094..10831
                     /gene="ccmC"
                     /locus_tag="ACHXWD_002116"
     CDS             10094..10831
                     /gene="ccmC"
                     /locus_tag="ACHXWD_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR7621033.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10828..11037
                     /gene="ccmD"
                     /locus_tag="ACHXWD_002117"
     CDS             10828..11037
                     /gene="ccmD"
                     /locus_tag="ACHXWD_002117"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR7621034.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            11034..11513
                     /gene="ccmE"
                     /locus_tag="ACHXWD_002118"
     CDS             11034..11513
                     /gene="ccmE"
                     /locus_tag="ACHXWD_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR7621035.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11510..13453
                     /gene="ccmF"
                     /locus_tag="ACHXWD_002119"
     CDS             11510..13453
                     /gene="ccmF"
                     /locus_tag="ACHXWD_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR7621036.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13450..14007
                     /gene="dsbE"
                     /locus_tag="ACHXWD_002120"
     CDS             13450..14007
                     /gene="dsbE"
                     /locus_tag="ACHXWD_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR7621037.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            14004..15056
                     /gene="ccmH"
                     /locus_tag="ACHXWD_002121"
     CDS             14004..15056
                     /gene="ccmH"
                     /locus_tag="ACHXWD_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR7621038.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15091..15738)
                     /gene="narP"
                     /locus_tag="ACHXWD_002122"
     CDS             complement(15091..15738)
                     /gene="narP"
                     /locus_tag="ACHXWD_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR7621039.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            16058..>16425
                     /locus_tag="ACHXWD_002123"
     CDS             16058..>16425
                     /locus_tag="ACHXWD_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR7621040.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         3578 a         4124 c         4775 g         3948 t
ORIGIN      
        1 tgttaccgtt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
       61 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
      121 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
      181 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
      241 tgaccatggt ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaacaacg
      301 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
      361 gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
      421 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
      481 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
      541 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
      601 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
      661 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
      721 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
      781 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
      841 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
      901 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
      961 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
     1021 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
     1081 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
     1141 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
     1201 atttcgatct ggtgaaatac ttggtgagtc aggtgatgtt gagtgaagag gatcgttttg
     1261 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
     1321 ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta
     1381 ctgaagtggt cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg
     1441 cgtcaaccgc tgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
     1501 ccagcccgca atggcaggct acgttgaaag cgatcgttcc atcttatgga cgcaagctga
     1561 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctgggcctga
     1621 aatacgacaa gccgcaggca gcagatagta cgccgaaacc gcagttgaaa ccgcaacccg
     1681 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
     1741 ggatgtggcg taaccgcctt atccgaccta cggggtggag cacttcacag cggtgtaggc
     1801 ctgataagac gcgtcagcgt cgcatcaggc aaccggctcg ggcgttagat gtcggatgcg
     1861 gcgtaaccgc cttatccaac ctacggggtg gagcacttca cagcggtgta ggcctgataa
     1921 gacgcgccag cgtcgcatca ggcaaccggc ttgggcgtta gatgtcggat gcggcgtgac
     1981 cgccttatcc gacctacggg gtgacgcact tcacagcagt ttagcgaact accgcgttgt
     2041 caattttctc ttccgccttc cagacgcggt acttcacatc tacattgtct ggcgtataca
     2101 ccacgatcgg cagcttgctg ttgtaacgca gcattccagc atcgcccaga tacgcggtga
     2161 caaatttctt ctctttcttg ccatccgggc aggccatcat cgtggaaacc ggcgagctaa
     2221 ctttatcaaa gacataataa tcatagcccc agccttccag cgttttgttt tccagcttcc
     2281 cgccgagacg atgcaaattg caatcgactt ccagcgtctg accggttaac agttctactt
     2341 tcagggtaga ttcatcttct tgcggggtta actgaatcac ctgacgcttc atcccttttt
     2401 cagcttgtgg ataaggcgcg attttttcca gtggctggac gctttctgcc gcccaggcgg
     2461 aagtggtggc gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt
     2521 ccttcttcat ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat
     2581 ttattaattt gctttatgaa taacttaaag aacattcaga caattttacc tgctctttta
     2641 gtgttaaatt ctgatgaggg agcaaaccag cagaaatatc tctggtgaat taatcgctat
     2701 ataaatatgt ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc acgcttcccg
     2761 ccactctttt gatcctgcta caggttttac cccgatcggg gtatgcatct ttgacacatc
     2821 ctttaatatc ttagcggcta taaaaatggc ttattaatta tgcggtttat ttggtcgctc
     2881 tcaattttca gagcgcgtta atgatggaag gtcaatgtga agattgatgc atcccgtcgg
     2941 ggcatactca ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc ctggtccggt
     3001 gatgaatctc attttctgac ccattgcacc cgttgcgacg cctgtattaa tgcttgtgaa
     3061 aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa aaataacgag
     3121 tgcagcttct gttacgcctg cgcgcaggct tgccccgaat cgctattttc tccgcgccac
     3181 accagggcct gggatttgca gttcaccatt ggggacgcgt gcctggcgta tcagtcagtt
     3241 gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa ttatctttcg cccgacgctc
     3301 tccgggatct accagccgca acttaatagc caactttgca acggatgtgg agcatgtgca
     3361 gccagctgtc cggtatcagc cataactgcg gagtatctcc atgcacacta actggcaagt
     3421 ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca gacatcagca cccaactgaa
     3481 cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt tgattgtggt
     3541 ggtggaagca gaagacagcg aaacgctgat ccaaaccatt gagtcagtac gcaacgtaga
     3601 gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg aggaaacacc
     3661 atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc tgcggcggct
     3721 gccggtctca gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga agccattaaa
     3781 tgggataaag cgccgtgccg tttctgcggt actggttgcg gcgttctggt cggaacgcag
     3841 caggggcgtg tggtggcctg tcagggcgac ccggacgcac cggttaaccg tggcctgaac
     3901 tgcattaagg gctatttcct gcccaagatc atgtacggta aagaccgttt gacgcagccg
     3961 ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg aatttacccc aatcacctgg
     4021 gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga aaaagggccg
     4081 gaatcgatcg gtatgttcgg ttctggtcag tggactatct gggaaggtta tgccgcgtcc
     4141 aagctgttta aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg tcactgtatg
     4201 gcgtcggcag tagttggctt tatgcgtacc tttggtatgg atgagccgat gggctgctat
     4261 gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg caaacatggc ggagatgcac
     4321 ccgatcctct ggtcgcgcat cactaaccgt cgtctctcta atcagaacgt caccgtggcg
     4381 gtgctttcta cctaccagca tcgtagcttc gagctggcgg ataacggcat catctttacg
     4441 ccacagtctg acctggtgat cctgaactac atcgccaact atatcattca aaacaatgcg
     4501 ataaatcagg acttcttcag caaacacgtt aacctgcgca aaggggcgac ggacatcggc
     4561 tacggtttac gtccgaccca tccgctggaa aaagcagcga agaatccagg ttctgacgcc
     4621 tccgaaccga tgagctttga agattacaaa gccttcgttg ccgagtatac gctggaaaaa
     4681 accgctgaaa tgaccggcgt gccgaaagac cagttagaac aactggcgca gctgtatgcc
     4741 gatccgaaca agaaagtcat ctcctactgg acgatgggct tcaaccagca tactcgtggc
     4801 gtgtgggcta acaacctggt ctacaacctg cacctgctga ccggcaaaat ttcccagccg
     4861 ggttgcggtc cgttctccct gaccgggcaa ccttctgcgt gtggtactgc gcgtgaagtg
     4921 ggcacctttg ctcaccgtct gcctgcggac atggtggtga ccaacgagaa acaccgtgat
     4981 atctgcgaga agaagtggaa tatcccgagc ggcaccattc cggcgaaaat cggtctgcac
     5041 gcggtggcac aagaccgtgc gctgaaagac ggcaaactga atgtttactg gaccatgtgt
     5101 accaacaaca tgcaggccgg gccgaacatc aatgaagagc gtatgccggg ctggcgcgat
     5161 ccgcgcaact tcatcatcgt ctccgatccg tatccgacag tcagtgcgct ggccgccgac
     5221 ttgatcctgc cgaccgcaat gtgggtagag aaagagggtg cttacggtaa cgccgaacgc
     5281 cgtactcagt tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc ggatctctgg
     5341 cagttagtcc agttctcccg ccgcttcaaa actgaagaag tgtggccgga agagctgcta
     5401 gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag tgctttatgc cacaccggaa
     5461 gtgagcaaat tcccggtatc cgaactggcg gaagatcagc tgaacgatga atcccgcgag
     5521 ctgggcttct atctgcaaaa agggctgttc gaagagtacg catggtttgg tcgcggtcac
     5581 ggtcacgatc tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg ctggccggtg
     5641 gtgaacggta aagaaacgca gtggcgttac agcgaaggta acgacccgta cgtgaaagcg
     5701 ggcgaaggct ataagttcta cggtaaaccg gatggtaaag cggtgatctt cgcgctgccg
     5761 ttcgaaccgg cggcggaagc accggatgaa gagtacgacc tgtggctctc taccggacgc
     5821 gttctggagc actggcacac tggcagtatg actcgccgtg tgccggaact gcaccgcgcc
     5881 ttcccggaag cggtcttgtt tattcacccg ctggatgcga aagcgcgcga tctgcgccgt
     5941 ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt tgaaacgcgc
     6001 ggtcgtaacc gtccgccaca gggcctggtg tacatgccgt tcttcgacgc cgcacagctg
     6061 gttaacaaac taacgctgga cgcgaccgat ccgctctcga aagagacgga cttcaagaag
     6121 tgcgcagtca aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa acctcaaaat
     6181 ggtcgccgcc gctttctgcg cgatgttgtt cgcacagcag gcgggctggc tgccgtgggt
     6241 gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt gcgcccgccc
     6301 ggagccataa acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca gtgtgttcag
     6361 gcttgccctt acgacacctt aaaactggcg acgctggctt ctggcctgtc ggcgggcacg
     6421 ccatattttg tcgcacggga tattccttgc gaaatgtgtg aggacattcc gtgcgccaaa
     6481 gtgtgcccga gtggtgcgct ggatcgtgag atcgaatcga tcgacgacgc gcggatgggg
     6541 ctggcggtgc tggtggacca ggaaaactgt ctcaactttc aggggctgcg ctgcgatgtt
     6601 tgttatcgcg aatgcccgaa aattgatgag gccatcaccc tggagctgga gcgcaacacg
     6661 cgtaccggta agcacgcccg ctttctgccg acggttcaca gcgacgcctg taccggctgc
     6721 ggtaagtgcg aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt accgctgtca
     6781 ctggcgaaag gggagttagg tcaccattac cgcttcggct ggctggaggg gaacaatggc
     6841 aaatcgtaaa cgtgacgccg ggcgcgaggc gctggtgaaa aaaggctggt ggcgcagtca
     6901 ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt ttttgagcgg
     6961 tccgtggttt ggtgtgtgga tcttacacgg caactacagc tgtagcctgt tattcgacac
     7021 cgttccgctg accgatccgc tgatgacgct gcaaagtctt gccagtggtc atctgcctgc
     7081 cacggtggcg ttgaccggtg cggtgataat cactgtgctc tacgccctgg caggaaagcg
     7141 attattttgc agctgggtct gcccgctgaa cccgattacc gacttagcga actggttacg
     7201 caggcgcttt gacctcaatc agtctgcgac gatccctcgc cacattcgct acgtgctgtt
     7261 ggtggtcatt ctgctgggat cagccttaac cggcacgctc atctgggaat ggatcaaccc
     7321 cgtttccctg atggggcgta gcctggtaat gggcttcggc agcggcgcgc tgcttattct
     7381 cgcgctgttt ttatttgatc tactggtcgt tgaacacggc tggtgcgggc acatttgtcc
     7441 ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg attaccgttg cggccagcga
     7501 tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt tgcccggaac cgcatgtgct
     7561 acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc accagccgcg attgcatgac
     7621 gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt acaataacta cacgatggag
     7681 ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca atggacggcg
     7741 atgctggccc tggtggtaag cggcgcggtt tgggcggcta atggcgtcga ttttagccag
     7801 tcgccggaag tatccgggac gcaggaaggg gccattcgga tgccaaaaga gcaggatcgg
     7861 atgccgctga actatgtgaa tcagccgcca atgatcccgc atagcgttga aggttatcag
     7921 gtaacgacca ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta tcgcaccact
     7981 ggcgcgccgc gtatcagtcc tactcacttt atggacagcg acggcaaagt gggcgcggaa
     8041 gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga taccgcgcca
     8101 atcgtgggga atacctttac cccatcaaaa ggttacggga aataagaggt tattatggga
     8161 aattctgacc gtaagcctgg tctgattaag cgcctgtgga aatggtggcg tacccccagc
     8221 cgtctggcgc tgggaacgct gctgttgatc ggttttgtcg gcggcatcgt cttctggggc
     8281 ggctttaaca ccggaatgga aaaagccaat accgaagagt tctgcattag ctgccacgaa
     8341 atgcgtaaca cggtgtatca ggaatacatg gattccgtgc actacaacaa ccgtagcggc
     8401 gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa gatgatacgc
     8461 aagctcaaag caagtaaaga gctgtatggt aaaatttttg gcgttattga cacgccgcag
     8521 aaatttgaag ctcatcgtct gacaatggca cagaatgagt ggcggcgcat gaaggacaat
     8581 aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata tggatacaac cgcccagaaa
     8641 tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg tattgattgc
     8701 cataaaggga tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg tttttaacag
     8761 ggttattgcg tgggtatgct tgaagccaga gagttacttt gtgagcggga tgaacgaacc
     8821 ttatttagtg gcttgtcatt tacgctgaac gcaggagagt gggtacaaat caccggtagc
     8881 aacggcgcgg ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg ccctgacgca
     8941 ggcgaggttc tctggcaagg gcagcccttg catcaggtac gcgacagcta ccatcaaaac
     9001 ctgttatgga taggccatca gccggggatc aaaacccggc tgacggcgtt agaaaatctg
     9061 cacttttatc atcgcgatgg cgataccgca caatgtctgg aagccctggc gcaggccggg
     9121 cttgccggat tcgacgatat tccggtaaat caactttctg ccgggcaaca acgccgcgtc
     9181 gctttagcgc gtctgtggct gacccgtgcc acgttatgga tcctcgacga gccttttacc
     9241 gcgattgacg ttaacggcgt cgatcgtctg acccagcgta tggcgcagca tacggagcag
     9301 ggggggattg tgattctgac tacccaccag ccgctcaacg ttgctgaaag taaaattcgc
     9361 cgcatttcac tgacgcaaac gagggccgca tgatgttctg gcgcattttc cgtcttgagc
     9421 tgcgtgtagc gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc ttcctgattg
     9481 taattaccct ttttccgctc agtatcggtc cggagccgca actgctggcg cgtattgcac
     9541 cgggcattat ctgggttgct gcgctgcttt catccttgct ggcgctggaa cgactgttcc
     9601 gtgacgattt gcaggacggc agtcttgaac aattgatgtt gttgccgtta cccttgcccg
     9661 ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac cggtctgccg ttactcatcc
     9721 tttcgccact ggtagcaatg ctactgggaa tggatgttta tggctggcaa gtgatggcgc
     9781 tgacgctgct gctgggaacg cctacgcttg gctttctcgg tgcaccgggt gtggcgttga
     9841 cagtgggact taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg ctgactatcc
     9901 cattactcat ctttgccacc gccgcgatgg acgcggcttc catgcatttg cccgttgacg
     9961 ggtatctggc aattttaggc gcgttgctgg caggcaccgc gacattaagt ccttttgcga
    10021 cggcggcagc gttacgaatc agcattcaat aacgctggat tactgatttt tgttcgagtc
    10081 tggtatcgaa actatgtgga aaacactgca tcaactggcg atcccaccac ggctgtatca
    10141 aatctgtggc tggtttatac cgtggctggc aattgccagt gtggtcgtgc ttaccgtcgg
    10201 ctggatctgg ggattcggct ttgctccggc tgattatcag cagggaaata gctaccgcat
    10261 tatctacctg catgtgcctg cggcgatctg gtcgatgggc atttatgcat caatggcagt
    10321 ggcagcgttt attggccttg tctggcagat gaaaatggcc aacctggcgg tggcggcgat
    10381 ggcccccatt ggtgccgtgt ttacctttat tgccctggtt accggctctg catggggaaa
    10441 accgatgtgg ggcacctggt gggtatggga tgcacgtctg acttctgaac tggtgctgct
    10501 gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc gtctggcggg
    10561 ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta ttcattactc
    10621 cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa gtatcgatcc
    10681 ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt ctgccacgct
    10741 gacgctgatg cggatgcgta atttgatttt gctgatggaa aaacgccgtc cgtgggtgag
    10801 tgaactgata ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg gaatgaattt
    10861 ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg tggtgatgac cgttattccg
    10921 ctggtggttt tggtcgtgca ctcggtgatg caacatcgcg caattctgcg tggtgtggcg
    10981 caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc tgcatgaata
    11041 ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg gcgctgacta
    11101 tcggtctggt gctatatgcg ctgcgctcga atatcgatct cttttatacg ccgggggaaa
    11161 ttctctacgg caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt ttgcgcgttg
    11221 gcgggatggt gatgctgggt agtgtgcagc gcgatcccaa ttcgctgaaa gtgaccttca
    11281 ctatttacga tgctgaaggc tcagtggatg tctcttacga aggcattttg ccggatctgt
    11341 tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat catatcctcg
    11401 cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc gccagaagtt gagaaagcga
    11461 tggaagctaa ccaccgtcgc ccggcgagtg tttataagga cccagcatca tgatgccgga
    11521 aattggaaac gggctgctgt gtctggcgtt agggattgcg ctgctgctgt ccgtgtatcc
    11581 gctatggggc gtggcgcgcg gagatgcgcg catgatcgcg tcttcccgct tgtttgcctg
    11641 gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt tcgtggtcaa
    11701 cgactttacc gtcacctatg ttgccagcta ctccaatacc cagcttccgg tgtggtatcg
    11761 cgtggcggct acctggggcg cgcatgaagg ctcgctactg ctgtgggtgc tgctgatgag
    11821 cggctggacc tttgcggtgg cgatttttag tcagcgtatt ccgctggata ttgtggcccg
    11881 cgtactggcg ataatgggga tggtcagcgt cggctttttg ctgttcattc tctttacctc
    11941 taacccattc tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc ttaacccgct
    12001 gttacaggac ccggggctga tcttccatcc gcctctgctt tatatggggt acgtgggttt
    12061 ctcggtggcg tttgcttttg ccattgcttc tttgctgagc gggcgtctgg acagcactta
    12121 tgcgcgtttt actcgcccgt ggacgctggc ggcgtggatt ttcctgacgc tcggcattgt
    12181 gctcggttcc gcatgggcct attacgaact cggctggggg ggctggtggt tctgggatcc
    12241 ggtagaaaac gcctcgttta tgccgtggct ggtggggact gcgctgatgc actcactggc
    12301 ggtcactgaa caacgcgcca gcttcaaagc gtggacatta ctgctggcaa tcagtgcctt
    12361 ctcgttgtgt ctgttgggga ctttcctcgt gcgttccggc gtgctggtat cggtacacgc
    12421 gtttgcgtct gatccggcgc gcggtatgtt tatcctcgcc tttatggtgc tggtgattgg
    12481 aggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg taaacaatgc
    12541 gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg ccgcgatgct
    12601 ggtggtgttg ctggggacgc tgctgccgct ggtgcacaag caactgggac tgggcagtat
    12661 ttcgattggc gaaccgttct tcaacaccat gtttacctgg ctgatggtgc cgtttgcgct
    12721 actgctgggt gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta aaatccgcaa
    12781 tttattgatt atcgccttca tctcaacgct ggtgctgtct ctgctgttgc cgtggctgtt
    12841 tgagagcaaa gttgtggcga tgacggtgct cggcctggca atggcctgct ggattgcggt
    12901 gctggcaatt gcggaagctg cgctgcgtat ttcacgcggc acgaaaacca ccttcagtta
    12961 ttgggggatg gtggctgctc acctggggct ggcagtgaca attgttggca ttgcttttag
    13021 ccagaactat agcgttgagc gtgatgtgcg catgaagtcc ggcgatagca tcgatattca
    13081 tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact ggcgtggcgg
    13141 tgtggcgact atcggcgtaa cgcgagaggg taaaccggaa acagtgctgt atgcggaaaa
    13201 acgttattac aacactgccg ggtcgatgat gaccgaagcg gcgattgacg gtggcatcac
    13261 gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg ctgtgcgtct
    13321 ttactacaaa ccatttgttc gctggatttg ggcgggcggg ctgatgatgg cgttgggcgg
    13381 actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa aaactgcgcc
    13441 ggaggctgta tgaagcgcaa agtattgtta attccgttga ttatcttcct ggcgattgcc
    13501 gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg atgatccgac caatctggaa
    13561 tcggcgctca ttggcaagcc agtgccgaag tttcgcctcg aatcactgga caatccgggg
    13621 cagttttatc aggcggatgt gctgactcag ggcaaaccag tgctgcttaa cgtctgggcg
    13681 acctggtgtc cgacctgccg tgcggaacat caatatctga atcagctttc tgcgcagggc
    13741 atccgcgtag tcggcatgaa ctataaagac gatcgccaga aggcaatcag ctggctgaaa
    13801 gagctgggca atccttacgc gctaagcctg tttgatggcg atggcatgtt agggctggat
    13861 ctcggtgtct atggcgcgcc agaaacgttc cttattgacg gcaacggcat cattcgctat
    13921 cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc gctgtgggag
    13981 aaatacagta aggaggccgc acaatgaggt ttttattggg catgctgatg ctgatgatct
    14041 ccggctcagc gctggcgacc atagacgtgt tgcagtttaa agatgaagca caggaacaac
    14101 agttccgtca gctcactgaa gaactgcgct gcccgaaatg ccagaacaac agcattgccg
    14161 attccaactc gatgattgcc accgacctgc gccagaaagt gtatgaactg atgcaggaag
    14221 gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg ttacggcaac ttcgtcactt
    14281 acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt gctgccagta gtagctattg
    14341 gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgtg gtgccggaag
    14401 cgtttcctga acaaagcgtg ccggaaggta agcgtgccgg atatgtcgct tatttgccgg
    14461 gcattgtggt ggcgttaatt gtggctggcg tcagctacta ccagaccgga aattatcaac
    14521 aggtgaaaat ctggcagcag gccacggcac aggctccggc gttgctggac agggcgctgg
    14581 atccgaaagc tgatccgctc aacgaagaag agatgtcgcg ccttgcgctg gggatgcgta
    14641 ctcaactgca aaaaaatccg ggagatatag acggctggat tatgttgggc cgcgttggca
    14701 tggcgctggg taacgccagt atcgccaccg atgcatacgc tactgcgtat cgcctcgatc
    14761 cgaaaaacag tgatgccgca ctgggttatg ctgaagcgtt gacacgttca tctgatccca
    14821 acgacaaccg cctcggcggc gaactgctac gtcagctggt gagaacggac catagcaata
    14881 tccgtgtgct aagcatgtat gcgtttaatg cctttgagca gcagcgattt ggcgaagccg
    14941 ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa cgatactcgc cgtgcggtga
    15001 ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt aaataaaaag
    15061 aggcccgatg gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt gcaggaacag
    15121 aatggtggcc gccacgcgtg agcggacatt gagtttacgc agcagatttc gaatatgaac
    15181 ttttactgtc tgctcggaaa tattcaacac agaggcaatc tgtttatttg acagcccctg
    15241 tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg gatcttcttc
    15301 cgcgccaaac atttcacgtt cacgtaagta ctgattgacg cgttcgctaa agactttgct
    15361 gcctttcgct ccggcacgaa tcgcttccag caacacttcc gggtcgctgt ctttcagcag
    15421 ataaccgtct gcgcctgcgt ctatcagcgc aaagacatcg ctggaggcat cggatacggt
    15481 caggataata atttgcgcgg taacgccatc cctgcgcaag gcattgaggg tatccaggcc
    15541 actcatacct ttcatattga gatccagcaa gatcacgtcg atatccagtc tattcgccag
    15601 atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag gatcaagctc
    15661 cagtaactga cgaacaccgc gtcgcataag tggatgatca tccacaatca tcacctgaaa
    15721 aggcgttgct tcaggcatag tagtctcctg aggttttatt agaatcatta tgtttgagcg
    15781 tagcgttggt aacgctacca ggcgcgtgaa tgttacccca ttttatgtat ggacatgtag
    15841 tgaaaactac aaccttgccg ttcgctttgg catcattttc attataaaga cggaattttg
    15901 catcagcttc tcatcaagaa aaacaactaa aaaatcaatc acaatacttg tgcatttctt
    15961 taaacagttt ttaaaaactg actccgggta tggagctatg ggtattttct gtacccaatg
    16021 cttttaacag caattaattt cataggatga aagctcaatg catcaatctg gttctgtttc
    16081 tctttgtcgt tccgcaatat ctgttctggt ggctacagcg ttatattcac ccatagcatt
    16141 ggcatcaaca gttgagtatg gtgagacagt tgatggtgtt ggcctggaaa aagatatcca
    16201 gctggtttat gggaccgcca ataatacgaa aatcaatcct ggcggagaac agcatataaa
    16261 agaatttggt gtaagtagta atactgaaat taacggcggg tatcagtaca ttgaaatgaa
    16321 tggcactgca gaatactcag tattaaatga tggttatcaa attgttcaaa tgggtggcgc
    16381 ggtaaaccag actacgctca ataatggagt gctacaggtt tatgg
//
LOCUS       DAZSBF010000084        16260 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZSBF010000084 DAZSBF010000000
VERSION     DAZSBF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.085"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(91..1293)
                     /gene="ubiI"
                     /locus_tag="ACHXWD_002124"
                     /gene_synonym="visC"
     CDS             complement(91..1293)
                     /gene="ubiI"
                     /locus_tag="ACHXWD_002124"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR7621041.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1316..2494)
                     /gene="ubiH"
                     /locus_tag="ACHXWD_002125"
                     /gene_synonym="visB"
     CDS             complement(1316..2494)
                     /gene="ubiH"
                     /locus_tag="ACHXWD_002125"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR7621042.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2491..3816)
                     /gene="pepP"
                     /locus_tag="ACHXWD_002126"
     CDS             complement(2491..3816)
                     /gene="pepP"
                     /locus_tag="ACHXWD_002126"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR7621043.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3842..4420)
                     /gene="ygfB"
                     /locus_tag="ACHXWD_002127"
     CDS             complement(3842..4420)
                     /gene="ygfB"
                     /locus_tag="ACHXWD_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR7621044.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4588..4917
                     /gene="zapA"
                     /locus_tag="ACHXWD_002128"
     CDS             4588..4917
                     /gene="zapA"
                     /locus_tag="ACHXWD_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR7621045.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4959..5142
                     /gene="ssrS"
                     /locus_tag="ACHXWD_002129"
     ncRNA           4959..5142
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHXWD_002129"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5163..5765
                     /locus_tag="ACHXWD_002130"
     CDS             5163..5765
                     /locus_tag="ACHXWD_002130"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR7621046.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5866..5925)
                     /locus_tag="ACHXWD_002131"
     CDS             complement(5866..5925)
                     /locus_tag="ACHXWD_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR7621047.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6257..6316)
                     /gene="ibsC"
                     /locus_tag="ACHXWD_002132"
     CDS             complement(6257..6316)
                     /gene="ibsC"
                     /locus_tag="ACHXWD_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR7621048.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6545..7777)
                     /gene="serA"
                     /locus_tag="ACHXWD_002133"
     CDS             complement(6545..7777)
                     /gene="serA"
                     /locus_tag="ACHXWD_002133"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR7621049.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8033..8692)
                     /gene="rpiA"
                     /locus_tag="ACHXWD_002134"
     CDS             complement(8033..8692)
                     /gene="rpiA"
                     /locus_tag="ACHXWD_002134"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR7621050.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9122..10015
                     /gene="argP"
                     /locus_tag="ACHXWD_002135"
     CDS             9122..10015
                     /gene="argP"
                     /locus_tag="ACHXWD_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR7621051.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10218..12362
                     /gene="scpA"
                     /locus_tag="ACHXWD_002136"
     CDS             10218..12362
                     /gene="scpA"
                     /locus_tag="ACHXWD_002136"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR7621052.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12355..13350
                     /gene="meaB"
                     /locus_tag="ACHXWD_002137"
     CDS             12355..13350
                     /gene="meaB"
                     /locus_tag="ACHXWD_002137"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR7621053.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13361..14146
                     /gene="scpB"
                     /locus_tag="ACHXWD_002138"
     CDS             13361..14146
                     /gene="scpB"
                     /locus_tag="ACHXWD_002138"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR7621054.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14170..15648
                     /gene="scpC"
                     /locus_tag="ACHXWD_002139"
     CDS             14170..15648
                     /gene="scpC"
                     /locus_tag="ACHXWD_002139"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR7621055.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15645..>16260)
                     /locus_tag="ACHXWD_002140"
     CDS             complement(15645..>16260)
                     /locus_tag="ACHXWD_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7621056.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3947 a         4382 c         4139 g         3792 t
ORIGIN      
        1 tagtttattt caaatgagga aaatctcccg gcgaaaaaac cgggagatga aagtgtgatg
       61 ggtatcaaat aaacagcaga agagaaattt ttaacgcagc cattcaggca aatcgtttaa
      121 tcccattgcc tggcggataa gttgtggttt aacgccagga agcgtgtcgg ccagtttcaa
      181 accaatatca cgcagcagtt ttttcgccgg attggtaccg gaaaacagat cgcggaatcc
      241 ctgcatacca gccagcatca gcgccgcact gtgcttgcgg ctacgctcat accggcgcag
      301 gtaaatgtac tgcccgatgt ctttcccctg acgatgcaac cgtttcagtt cagcaatcag
      361 ctctgcagca tccataaagc caagatttac tccctgcccc gccagcgggt gaatggtatg
      421 cgcggcgtcg cccaccagca ccagacggtg cgcggcaaac tggcgcgcat aacgccccgt
      481 cagtgggaac acctgacgcg cgctctcaac cttgcataag cccaggcgat tatcaaaagc
      541 gatatttaac gcgcgattaa attcatcttc acttgcctgc tgcatccgct gcgcttcctc
      601 tggcgacagt gaccagacaa ttgagcaaag atgcggatcg ctaagcggta aaaaggccag
      661 aatgccttcg ccatggaaaa cctgccgcgc caccgcatca tgcggttctt ccgtgcgaat
      721 ggtcgctacc agcgcgtgat gctgataatc ccagaaagtc agcggaatat ctgctttgtt
      781 gcgcaaccag gaattagcac cgtccgcgcc aatcaccaga cgcgccgtta acatgctgcc
      841 atctttcagc gtcaggaaag tttcattttc tccccaggcg acctgctgta attctgcggg
      901 ggccaacaga gtgatatctg acgactgctg cgctttgttc cacagcgcgt agtgaatcac
      961 tgagttttca acgatatgcc caagatggct atagcccatg ctttgatcgt caaacgaaat
     1021 gtgaccaaaa ctgtctttgt cccacacttc cataccgtga taacaactgg ccctacgaga
     1081 gagaatgtcc tgccagacgc caagacgggt gagtaatttt tcgctggcgg cattgatagc
     1141 cgaaacgcgc agttgtggtg gtgcatccgc cgccagaggt tccggtacgc gctgctccag
     1201 tacggcaacg cgtaagccgc tcccctgtaa gccgcaggca accgccagcc ccaccatgcc
     1261 gccaccaaca atggctacat caacactttg cattgtttat tccttaaaac cgccttcaac
     1321 gcgccaccca accgagggtg cgctgcgcca gcacatcgcg tgccggggtg aataattcca
     1381 tcgtcatcag tccgatgttg cgcccgacaa ccagcggtgc ccaacggttg gcaaaaagat
     1441 gtaccaggct gtccgtgacg ccgatggttg cttcgcgatc gctctgtcga cgctgctgat
     1501 agcggcacaa tatgccgtaa tcaccgatgt cttctccgcg ctcatgcgcc tgagtcaggg
     1561 tttccgcaag gctcatgaca tctcgcatac cgaggttaaa cccttgcccg gcaatcgggt
     1621 gcagagtttg cgccgcattg cccaccagca cggtacgatg ggtaatagct ctggcggcgc
     1681 gggttaacgc cagcggataa gcactgcgtt taccggcgtg ggtaattttt ccaagtcgcc
     1741 agccaaaggc cgactggagt tcacggcaaa acttctcgtc gctccacgac agcacctctt
     1801 cgcgccgttc cagtggatga caccagacca gcgaacagcg tccgtcagac atcggcaaca
     1861 tcgccagcgg gccatgttgc gtgaagcgtt caaaagcgcg gccttcgtgc gcaacggaag
     1921 tcgcaacgtt ggcaatcacg gccagttgtt cgtaaggctc ctgctgccag tcaacgccgc
     1981 acgcggtggc taacgctgaa tgggtgccat cagctgctac cagcacgcgg cccgtcagcg
     2041 tctcgccact ctccagcgtc acttcaacgt gactctgagt acgggcaacg ttagccacgc
     2101 gatcagggca atgcagcgtt acgccaggtg ctttacgcag caatgcaaac agccgttgcc
     2161 cgacattgtg caattcgaca acctgtccca gcgccgccag ttggtaatct tctgcggcga
     2221 gggtgacaaa tccagcgtga ccacgatcgc tgacatgcac ggtagtgatg gcggttgcgt
     2281 aatccgccag agattgccag acgccgatgc gcgccagttg ctgacaggtc cccgcagcca
     2341 gcgcaatagc tcgtccatca aagcccggat gagcatgtga ctctggcgcg gtcgcttcaa
     2401 tcaaatgtac cggcagcgcc ccgtgactta accgggaaat agccagcgcc agcgtcgcgc
     2461 ccgccatgcc gccaccgacg atgattacgc tcattgcttt ctcgcagctg ccatcaacgc
     2521 ttcgatttct tccggctttt tcaccacgct ggcggtgagg ttttcgttgc cggtttcggt
     2581 aatcacaatg tcgtcttcaa tacgaatgcc gataccgcga tattgttctg gcacatccgc
     2641 atccggcgcg atatacagac caggctctac ggtcagtacc atccccggtt ccagaatgcg
     2701 cgagcgatcc tgaccataaa cgccaacgtc atggacatcc agtcctaacc agtggctaag
     2761 gccatgcata aagaaaggac gatgggcgtt ctgagcgatc agttcatcaa catcaccttt
     2821 caggatgccg agttttacca ggccgctaac catgatgcgc accacttcac cagtgacttc
     2881 cagaatggaa gttcccggac gatacaggcg caggctggtt tcgagagact ccagcacaat
     2941 gtcgtagatt tcacgctggg cctgggtgaa tttgccgttg accgggaagg tgcgggtaat
     3001 atcgcccgcg taacctttgt attcacaacc cgcgtcaatc aacaccaggt caccgtcgcg
     3061 cagttcactc tcgttttcgg tgtagtgcag aatgcagccg ttttcaccgc tgccgacaat
     3121 ggtgttatag gacggatagc gcgcaccgtg gcggttaaat tcgtggtgaa tttcgccttc
     3181 cagatggtac tcgaacattc ccggacggca tttttccatc gcccgtgtat gtgccatggc
     3241 ggtgatttct cccgcgcggc ggagtacggc aatctcttct ggcgatttga acaggcgcat
     3301 ttcatgaaca acaggacgcc agtcgatcat cgttgccggt gcggtgagat tttgccgcga
     3361 acctttacgc agtttttcca gcgcactgtt catgatttca tcagcatatg catattcgcc
     3421 ctgggcatgg taaaccacat ccaggccgtt aagtagttga taaagttgct gattgatttc
     3481 gctgaatgcc agtgcgcggt caacgcccag tttctctggc gcggcatcct ggcctaagcg
     3541 acggccaaac cagatctccg ccgtcaggtc gcgaacgcgg ttaaacagaa cgctgtggtt
     3601 atgagtgtca tcgcttttaa tcagcaccag caccgcttcc aattcgttaa agccggtgaa
     3661 gtaccagaag tcactgttct gacgataggg gtattcgctg tcggcgctac gtgttacttc
     3721 tggtgcagca aaaatcagcg cggcgctgcc gggttgcatt tgctccacca gggcctgacg
     3781 gcgacgctga aactcttgcc gggatatctc actcataaca ctctccttac gttttttgtt
     3841 tttagtgtag agtcggtttt tgtacttctg gcgcggtcgg ttgcggatga gtaaaggtgt
     3901 cgtggcataa cagcgcggca acacgaacgt attcgatgat ctcttcaagc gacatttcaa
     3961 gctcttcctg atcttcgtct tcgtcgtaac ccagttgcgc aatgttacgc agatcgtcga
     4021 tagcttcacc ggtttcgccg gtcactttat ccagcttcgg ttgcgtaacg ccaagaccaa
     4081 gcaggaagtg attgacccaa cctgccaatg catcagcccg atcgaaaacg ctgacatcat
     4141 cgccatcagg cagataaagc tgaaaaagga agccgtcatc ctgcagggca tcgctggtgg
     4201 cagagtgcat tttacgcagt gcctgtgcca gctcatgacc gaaagccatg ccttcgttcg
     4261 tcaggtcgtg aagtagcggc agccatgagc tgtcatcgtt accgccacat atcatcccac
     4321 tgattaaacc atgcatctcg gcaggggtca gccccgtccc ttgttggttc agatactggt
     4381 tcatttcgtt gtaaccaggc atttcgttct gtatagacat aagcattcgt cgtcaaaggg
     4441 aggaatattc atgatatgct accactttgg gccctggtgg accagaaaag ggcttgtctc
     4501 ttctcatcag ggtagctata gtgtcgcccc ttcgcagacc atgggtctaa agacgaaggc
     4561 agcgcagtca atcagcagga aggtggcatg tctgcacaac ccgtcgatat ccaaattttt
     4621 ggccgttcac tgcgtgtgaa ctgcccgcct gaccaaaggg atgcgttgaa tcaggcagcg
     4681 gacgatctga accaacggtt gcaagatctg aaagaacgca ctagagtcac aaatactgaa
     4741 cagttggtct tcattgccgc attgaatatc agctatgagt tagcgcaaga aaaagcgaag
     4801 actcgtgact acgcggcaag tatggaacag cgtattcgga tgctgcagca gaccatagaa
     4861 caagcgttac ttgaacaagg tcgcatcacc gaaaaaacta accaaaactt tgaatgacac
     4921 ttttcggttt actgtggtag agtaaccgtg aagacaaaat ttctctgaga tgttcgcaag
     4981 cgggccagtc ccctgagccg atatttcata ccacaagaat gtggcgctcc gcggttggtg
     5041 agcatgctcg gtccgtccga gaagccttaa aactgcgacg acacattcac cttgaaccaa
     5101 gggttcaagg gttacagcct gcggcggcat ctcggagatt cccttcttat ctggcaccag
     5161 ccatgacgca actaccagaa ctcccactga cattatcccg acaagaaatc cgcaaaatga
     5221 ttcggcaacg tcgtcgtgcg ttaacgccgg aacaacagca ggaaatgggt caacaagccg
     5281 ctacccggat gatgacttat cccccggtgg tgatggcgca tacggtcgct gtattcctct
     5341 cttttgatgg tgaactcgac acccagccgc taatagaaca actctggcgc gccggtaagc
     5401 gcgtatatct tccagttttg catcccttta gtgccggtaa tttgctgttc ctgaattacc
     5461 atccgcaaag cgaactggtg atgaacaggt tgaagatcca tgagccaaaa ctggatgtgc
     5521 gtgacgtgct acccctttcc cgattagacg tgctgatcac accgctggtc gcctttgatg
     5581 agtacggtca gcgcctggga atgggcggtg gtttttatga tcggacctta caaaactggc
     5641 agcactataa aacgcaaccg gtgggttatg cgcatgattg tcagttggtg gaaaaactcc
     5701 ccgttgaaga gtgggatatc cctcttcctg cggtggttac accgtcgaaa gtctgggagt
     5761 ggtaagggcg atacacctgc atcgccctga ttgacatcgt tgattcttta acctaattta
     5821 gtgagtaagg gtaagggagg attgctcctc ccctgtgact gactgttaat aagctgggaa
     5881 acttatgagt aacagtacaa tcagtatgat ggcaagtcgc atcataaccc ttctccttca
     5941 agccctcgct tcggtgaggg ctttaccgtt acagccccat gctgcccagc tatcgtaagc
     6001 tctctttaaa taaccacaac gcattgatct acctttaaat cttttcttcg agcgtgctcg
     6061 aaaaacccat gcaaaacaga gataattttt gggaaaaagg atctattacg gcagtaaacg
     6121 tgggcgatga agtggctatc aaaaagttcg gttcagggcg atgcaccccg catcgccctg
     6181 attgacatcg ttgattcttt gacctaattt agtgagtaag ggtaagagag gatgactcct
     6241 cccctgagac tgactgttaa taagctccga aacttatcag taacagtaca atcagtatga
     6301 tgacaagtcg catcataacc cttctccttt aagccctcgc ttcggtgagg gctttaccgt
     6361 tacagcccca tgctgtcccg ccatcgtaaa tccccattaa ataaacacaa cgcattgatc
     6421 tgagtttgat ttattttctg gagcagactc gcaaagtaga atgcgcaacg cggcaacggt
     6481 gtggagaagg gataaaaaaa cgggcaagtc agtgacctgc ccgttaattt tcagagaagg
     6541 ggaattagta cagcagacgg gcgcgaatgg tacccggaat agctttcatt gcctgcagcg
     6601 ctttttcggc aacgtcttcg tcagcttcaa tatcaataac tacataaccc atctgggcgg
     6661 aagtttgtag atattgcgcg gcgatgttga cgccctgctc ggcgaagatt ttgttcagcg
     6721 cagttagcac gcccggacgg ttttcgtgga tgtgcatcag acgacgacca ccatgcagtg
     6781 gcagcgagac ttccgggaag ttcaccgcag agagcgttga gccattgtca gaatacttga
     6841 tcaatttacc cgcaacttcc aggccgatat tctcctgcgc ttcctgagtc gaaccgccaa
     6901 tgtgtggcgt cagaaggacg ttgtcgaatt cacacagtgg agaggtaaat ggatcgctat
     6961 tggtcgccgg ttccgtcggg aatacgtcga ttgccgcccc cgccagatgt ttgctcgcca
     7021 gcgcatcaca cagcgccgga atatccacca cagtaccgcg cgaggcatta atcagcagcg
     7081 agccgggctt cattagcgaa atctctttcg cgcccatcat atttttggtg gacggattct
     7141 ctggtacatg cagactcacc acatcgctca tattcagcag gtcagaaaga tgctgtacct
     7201 gagtggcgtt gcccagcggc agtttgtttt caatatcata aaagtaaaca tacattccca
     7261 gcgattcagc cagaatgccc aattgcgtac caatatgacc gtagccgatg atacccagct
     7321 ttttgccgcg cgcttcaaaa gaacccgccg ccagtttgtt ccacacgcca cggtgcgctt
     7381 tagcattggc ttccggcacg ccgcgcaata gcagcagcag ttcgccaatc accagctccg
     7441 caacagagcg cgtatttgag aacggtgcgt taaataccgg gatcccgcgc tttgccgccg
     7501 catccagatc aacctggttt gttccgatac agaaacagcc aatagcgacc agtttttctg
     7561 cggcgttgat cacgtcttca gtcagatggg tacgggatcg caggccgatg aagtgggcat
     7621 cgcggatgga ttcttttaat tgttcatcat ccagcgcgcc tttgtgaaat tcgatgttgg
     7681 tgtaaccagc tgcacgaagg ctttccagcg ccttttggtg cacgccttct accagcagaa
     7741 acttaatctt gtctttctcc agcgatacct ttgccattta cccaatcctg tcttttgaaa
     7801 tgttgtgtgc ggatttgcat ccgcctttca acatatcaaa aaataatatt gcggcaatat
     7861 gaacgtttgc gtcgcgatgt tgaggaaata tcacgcaaag gtatttcttg gttgaatatg
     7921 ctggtttaga ggatttattt agagcaatcg acaatagcca agtaaaaacg tgacacatgt
     7981 caccaaattt aatgaagaga atttttttaa cgggggaggt tcccccgtca gatcatttca
     8041 caatggtttt gacaccgtca ggtgtgccaa tcagcgcaac gtccgcgcca cggttagcaa
     8101 acaagccaac agtcaccacg ccaggaatcg catttatggc gttttccatc gctatcgggt
     8161 caaggatttc catgccgtgg acgtcgagga tcacgttgcc gttatcggtc accacgccct
     8221 gacggtattc cggacgaccg cccagtttca ccagctggcg cgccactgca ctacgtgcca
     8281 tcgggataac ttctactggc agcgggaatt tgcccagaat atcaacctgc ttggaagcgt
     8341 cagcaataca gataaatttt tccgcaaccg aagcaatgat tttttcacgg gtcagcgccg
     8401 cgccgccgcc tttgatcatt tgcatgtggc cgttgatttc atctgcgcca tcaacgtaga
     8461 tgccaaggct gtcgacttcg ttgagatcaa aaacgtgaat gccgaggctt ttcagttttt
     8521 cagtggaagc atctgaactg gaaacggccc cttcaatctg gcctttcatt gtaccgagcg
     8581 cgtcaataaa gtgtgcggcg gtggaacctg tacctacacc aacaatggtg ccgggctgaa
     8641 catactgaag tgccgcccat cctactgctt ttttcaattc atcctgcgtc atgatcgttt
     8701 cgcctgtggt atgaaatttc acacgcatta tatacaaaaa aagcgattca gaccccgttg
     8761 gcaagccgcg tggttaactc atccataaaa tatcgcgcaa tggcgggcat cccctttcgc
     8821 cccgcaaata aagcatacaa cggtctgggt atgccgctcc acggtgcgaa caggcgcacc
     8881 agttcccccc gttcgcaagc ccctgtttac aggcaaattg aggcaataac gccaccccat
     8941 tcaacacaac cagggcacga actcgctgag cgaaatgcat tagcgcaaat ccctgatctt
     9001 ttaagctgcg cgtttaacca cggatacaaa tgttacccgc cgacggcttc ggtatatgca
     9061 acctgacaca aaattgtgtc atagtgcagg aaaaagcatt taccaggagc agacaacagc
     9121 aatgaaacgc ccggactaca gaacattaca ggcactggat gcggtgatac gtgaacgagg
     9181 atttgagcgc gcggcacaaa agctgtgcat tacacaatca gccgtctcac agcgcattaa
     9241 gcaactggaa aatatgttcg ggcagccgct gttggtgcgt accgtaccgc cgcgcccgac
     9301 ggaacaaggg caaaaactgc tggcactgct gcgccaggtg gagttgctgg aagaagagtg
     9361 gctgggcgat gaacaaaccg gttcgactcc gctgctgctt tcactggcgg tcaacgccga
     9421 cagtctggcg acgtggttgc ttccggcact ggctcctgtg ttggctgatt cgcctatccg
     9481 cctcaacttg caggtagaag atgaaacccg tactcaggaa cgtctgcgcc gcggcgaagt
     9541 ggtcggcgcg gtgagtattc aacatcaggc gctgccgagt tgtcttgtcg ataaacttgg
     9601 tgcgctcgac tatctgttcg tcagctcaaa accctttgcc gaaaaatatt tccctaacgg
     9661 cgtaacgcgt tcggcattac tgaaagcacc tgtggtcgcc tttgaccatc ttgacgatat
     9721 gcaccaggcc tttttgcagc aaaacttcga tctgcctcca ggcagcgtgc cctgccatat
     9781 cgttaattct tcagaagcgt tcgtgcaact tgctcgccag ggcaccacct gctgtatgat
     9841 cccgcacctg caaatcgaga aagaactagc cagcggtgaa ctgattgact taacgccagg
     9901 gctatttcaa cgccggatgc tctactggca ccgctttgct cctgaaagcc gcatgatgcg
     9961 taaagtcact gatgcgttgc tcgattatgg tcacaaagtc cttcgtcagg attaatccat
    10021 caaataatgc ctgatagcac atatcaggcg ttatcctcac ttctttttgt attccttgaa
    10081 tcacatcaca aaatagacaa atctcaggcg gcaaaaaacg acgtctgaat gcattttttt
    10141 gctggcgaca aacccacgta aaaagctcac cgtaagcgca aataccctca ttttgattgc
    10201 gttttacgga gcaaataatg tctaacgagc aggagtggca acagcttgcc aacaaggaat
    10261 tgagccgtcg ggagaaaact gtcgactcgc tggttcagca aaccgcggaa gggatcgcca
    10321 tcaagcagct gtataccgaa gccgatctcg ataatctgga ggtgacaggt acccttcctg
    10381 gtttgccgcc ctacgttcgt ggcccgcgtg ccactatgta taccgcccaa ccgtggacca
    10441 tccgtcagta tgctggtttt tcaacagcaa aagagtccaa cgctttttat cgccgtaacc
    10501 tggccgccgg gcaaaaaggt ctttccgttg cgtttgacct tgccacccac cgtggctacg
    10561 actccgataa cccacgcgtg gcgggtgacg tcggcaaagc gggcgtcgct atcgacaccg
    10621 tggaagatat gaaagtcctg ttcgaccaga tcccgctgga taaaatgtcg gtttcgatga
    10681 ccatgaatgg cgcagtgcta ccagtactgg cgttttatat cgtcgccgca gaagagcaag
    10741 gtgttacacc tgataaactg accggcacca ttcaaaacga tattctcaaa gagtacctct
    10801 gccgcaacac ctatatttac ccaccaaaac cgtcaatgcg cattatcgcc gacatcatcg
    10861 cctggtgttc cggcaacatg ccgcgattta ataccatcag tatcagcggt taccacatgg
    10921 gggaagcggg tgccaactgc gtgcagcagg tagcatttac gctcgccgat gggattgagt
    10981 acatcaaagc agcaatctct gccggactga aaattgatga cttcgctcct cgcctgtcgt
    11041 tcttcttcgg tatcggcatg gatctgttta tgaacgtcgc catgttgcgt gcggcacgtt
    11101 atttatggag cgaagcggtc agtggatttg gcgcacagga tccgaaatca ctggcgctgc
    11161 gtacccactg ccagacctca ggctggagcc tgactgaaca ggatccgtat aacaacgtta
    11221 tccgcaccac cattgaagca ctggctgcga cgctgggcgg tactcagtca ctgcatacca
    11281 acgcctttga cgaagcgctt ggtttgccta ccgatttctc agcacgcatt gcccgcaaca
    11341 cccagatcat catccaggaa gaatcagaac tctgccgcac cgtcgatcca ctggccggat
    11401 cctattacat tgagtcgctg accgatcaaa tcgtcaaaca agccagagct attatccaac
    11461 agatcgacga agccggtggc atggcgaaag cgatcgaagc aggtctgcca aaacgaatga
    11521 tcgaagaggc ctcagcgcgc gaacagtcgc tgatcgacca aggcaagcgt gtcatcgttg
    11581 gtgtcaacaa gtacaaactg gatcacgaag acgaaaccga tgtacttgag atcgacaacg
    11641 tgatggtgcg taacgagcaa attgcttcgc tggaacgcat tcgcgccacc cgtgatgatg
    11701 ccgccgtaac cgccgcgttg aacgccctga ctcacgccgc acagcataac gaaaacctgc
    11761 tggctgccgc tgttaatgcc gctcgcgttc gcgccaccct gggtgaaatt tccgatgcgc
    11821 tggaagtcgc tttcgaccgt tatctggtgc caagccagtg tgttaccggc gtgattgcgc
    11881 aaagctatca tcagtctgag aaatcggcct ccgagttcga tgccattgtt gcgcaaacgg
    11941 agcagttcct tgccgacaat ggtcgtcgcc cgcgcattct gatcgctaag atgggccagg
    12001 atggacacga tcgcggcgcg aaagtgatcg ccagcgccta ttccgatctc ggtttcgacg
    12061 tagatttaag cccgatgttc tctacacctg aagagatcgc ccgcctggcc gtagaaaacg
    12121 acgttcacgt agtgggcgca tcctcactgg ctgccgatca taaaacgctg atcccggaac
    12181 tggtcgaagc gctgaaaaaa tggggacgcg aagatatctg cgtggtcgcg ggtggcgtca
    12241 ttccgccgca ggattacgcc ttcctgcaag agcgcggcgt ggcggcgatt tatggtccag
    12301 gtacacctat gctcgacagt gtgcgcgacg tactgaatct gataagccag catcatgatt
    12361 aatgaagcca cgctggcaga aagtattcgc cgcttacgtc agggtgagcg tgccacgctc
    12421 gcccaggcca tgacgctggt ggaaagccgt cacccacgtc atcaggcgct aagtacacag
    12481 ctgcttgacg ccattatgcc gttctgtggt aacgccctgc gactgggcgt taccggcaca
    12541 cccggcgcgg ggaaaagtac ctttcttgaa gcctttggca tgttgttgat tcgtgaggga
    12601 ttaaaggttg ctgttattgc ggtcgatccc agcagcccgg tcactggcgg tagcattctc
    12661 ggggataaaa cccgcatgaa tgacctggcg cgtgccgagg cggcgtttat tcgcccggta
    12721 ccctcctccg gtcatctggg cggtgccagt cagcgagcac gggaattaat gctgttatgc
    12781 gaagcggcgg gttatgacgt agtgattgtc gaaacggttg gcgtcgggca gtcggaaacg
    12841 gaagtcgccc gcatggtgga ctgttttatc tcgttgcaaa ttgccggtgg cggcgatgat
    12901 ctgcagggca ttaaaaaagg gctgatggaa gtggctgatc tgctcgttat caacaaagac
    12961 gatggcgata accataccaa tgtcgccatt gcccggcata tgtacgagag tgccctgcat
    13021 attctgcgac gtaaatacga cgaatggcag ccacgggttc tgacttgtag cgcactggaa
    13081 aaacgtggaa tcgatgagat ctggcacgcc atcatcgact tcaaaaccgc gctaactgcc
    13141 agtggtcgtt tacaacaagt gcggcaacaa caatcggtgg aatggctgcg taagcagacc
    13201 gaagaagaag tactgaatca cctgttcgcg aatgaagatt tcgatcgcta ttaccgccag
    13261 acgcttttag cggtcaaaaa caatacgctc tcaccgcgca ccggcctgcg gcagctcagt
    13321 gaatttatcc agacgcaata ttttgattaa aggaattttt atgtcttatc agtatgtgaa
    13381 cgttgccact atcaacaaag tggcggtcat tgagtttaac tatggacgaa aacttaatgc
    13441 cttaagtaaa gtctttattg atgatcttat gcaggcgtta agcgatctca accggccgga
    13501 aattcgctgt atcattttgc gggcgccaag tggatccaaa gtcttctccg caggtcacga
    13561 tattcacgaa ctgccgtctg gcggtcgcga tccgctctcc tatgatgatc cattgcgtca
    13621 aatcacccgc atgatccaaa aattcccgaa accgatcatt tcgatggtgg aaggtagtgt
    13681 ttggggtggc gcatttgaaa tgatcatgag ttccgatctg atcatcgccg ccagtacctc
    13741 aaccttctca atgatgcctg taaacctcgg cgtcccgtat aacctggtcg gcattcacaa
    13801 cctgacccgc gacgcgggct tccacattgt caaagagctg atttttaccg cttcgccaat
    13861 caccgcccag cgcgcgttgg ctgtcggcat cctcaaccat gttgtggaag tggaagaact
    13921 ggaagatttc accttacaaa tggcgcacca catctctgag aaagcgccgt tagccattgc
    13981 tgttatcaaa gaagagctgc gtgtactggg cgaagcgcac accatgaact ccgatgaatt
    14041 tgaacgtatt caggggatgc gccgcgcggt ttatgacagc gaagattacc aggaagggat
    14101 gaacgctttc ctcgaaaaac gtaaacctaa tttcgttggt cattaatccc tgcgaacgaa
    14161 ggagtaaaaa tggaaactca gtggacaagg atgaccgccg atgaagcggc agaaattatc
    14221 cagcataacg acatggtggc atttagcggc tttaccccag cgggttcgcc gaaagcctta
    14281 ccgaccgcga ttgcccgcag agctaacgaa cagcatgagg ccaaaaagcc gtatcaaatt
    14341 cgcctgctga cgggggcgtc aatcagcgcc gccgctgacg atgtactttc tgacgccgat
    14401 gctgtttcct ggcgtgcgcc atatcaaaca tcgtccggtt tacgtaaaaa gatcaatcag
    14461 ggcgcggtga gtttcgttga cctgcatttg agcgaagtgg cgcaaatggt caattacggt
    14521 ttcttcggcg acattgatgt tgccgtcatc gaagcatcgg cactggcacc ggatggtcga
    14581 gtctggttaa ccagcgggat cggtaatgcg ccgacatggc tgctgcgggc gaagaaagtg
    14641 atcattgaac ttaatcacta tcacgatccg cgcgttgcag aactggcgga tattgtgatt
    14701 cctggcgcac caccgcggcg caatagcgtg tcgatcttcc atgcaatgga tcgcgtcggt
    14761 acccgctatg tgcaaatcga tccgaaaaag attgtcgccg tcgtggaaac caacttgccc
    14821 gacgccggta atatgctgga taagcaaaat cccatgtgcc agcagattgc cgataacgtg
    14881 gtcacgttct tattgcagga aatggcgcat gggcgtattc cgccggaatt tctgccgctg
    14941 caaagtggcg tgggcaatat caataatgcg gtaatggcgc gtctggggga aaacccggaa
    15001 attcctccgt tcatgatgta ttcggaagtg ctacaggaat cggtggtgca tttactggaa
    15061 accggcaaaa tcagcggggc cagcgcctcc agcctgacaa tctcggccga ttccctgcgc
    15121 aagatttacg acaatatgga ttactttgcc agccgcattg tgttgcgtcc gcaggagatt
    15181 tccaataacc cggaaatcat ccgtcgtctg ggcgtcatcg ctctgaacgt cggcctggag
    15241 tttgatattt acgggcatgc aaattcaacg cacgtagccg gggtcgatct gatgaacggc
    15301 atcggcggca gcggtgattt tgaacgcaac gcgtatctgt cgatctttat ggctccgtcg
    15361 attgctaaag aaggcaagat ctcaaccgtc gtgccaatgt gcagccatgt tgatcacagc
    15421 gaacacagcg tcaaagtgat catcaccgaa caagggatcg ccgatctgcg cggtctttcc
    15481 ccgcttcaac gcgcccgcac tatcattgat aattgtgcac atcctatgta tcgggattat
    15541 ctgcatcgct atctggaaaa tgcgcccggc ggacatattc accacgatct tagccacgtc
    15601 ttcgacttac accgtaattt aattgcaacc ggctcgatgc tgggttaatg gtttgtcccg
    15661 tcacgatctt cagcaatatg ctgaagcatc gtggcggtat aacgatgatt tctgattgaa
    15721 taaagatatt cctgcatata catcggcctg tgcggcgcat caaaatattt cagcgttgcc
    15781 ggattcacca gtcgccaggc aaaatgcggg atcaccgtca gaaaatgtcc gcgctccacg
    15841 gcgctgattt tggccataaa actgtaggga cgataaataa tggtgggatt gatcccacaa
    15901 ggtcgcatat tcgcatcaat cattgcttcg aagttagccc ggttttgaaa gcgcatttgt
    15961 agccagggca gctcctgaag cagctcttgc tcttgacgct cttcataacg gcgagaaacg
    16021 agaaagccca atcgcagcgg tggtaattca ctgatagtca ggttttccag ttcctgaact
    16081 cgtgcagata catgctgtgg ggagataata aaatctaact gacggtcaaa aagattatcg
    16141 ataacgccgt tttcgctgaa ctcaactggc tgggcggtca cgccttcata cttatctccg
    16201 agactaatca actgatcgaa aataattgtc ggataagtat tatctatgcc aatcacaatt
//
LOCUS       DAZSBF010000085        16181 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZSBF010000085 DAZSBF010000000
VERSION     DAZSBF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.086"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHXWD_002141"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="ACHXWD_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR7621057.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="ACHXWD_002142"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="ACHXWD_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR7621058.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="ACHXWD_002143"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="ACHXWD_002143"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR7621059.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="ACHXWD_002144"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="ACHXWD_002144"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR7621060.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="ACHXWD_002145"
     CDS             complement(5254..6294)
                     /locus_tag="ACHXWD_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR7621061.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="ACHXWD_002146"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="ACHXWD_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR7621062.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHXWD_002147"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="ACHXWD_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR7621063.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="ACHXWD_002148"
     CDS             7229..7669
                     /locus_tag="ACHXWD_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR7621064.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHXWD_002149"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="ACHXWD_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR7621065.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHXWD_002150"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="ACHXWD_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR7621066.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..10832
                     /gene="rsxC"
                     /locus_tag="ACHXWD_002151"
     CDS             8898..10832
                     /gene="rsxC"
                     /locus_tag="ACHXWD_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR7621067.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10833..11891
                     /gene="rsxD"
                     /locus_tag="ACHXWD_002152"
     CDS             10833..11891
                     /gene="rsxD"
                     /locus_tag="ACHXWD_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR7621068.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11895..12515
                     /gene="rsxG"
                     /locus_tag="ACHXWD_002153"
     CDS             11895..12515
                     /gene="rsxG"
                     /locus_tag="ACHXWD_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR7621069.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12519..13214
                     /gene="rsxE"
                     /locus_tag="ACHXWD_002154"
     CDS             12519..13214
                     /gene="rsxE"
                     /locus_tag="ACHXWD_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR7621070.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13214..13849
                     /gene="nth"
                     /locus_tag="ACHXWD_002155"
     CDS             13214..13849
                     /gene="nth"
                     /locus_tag="ACHXWD_002155"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR7621071.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14460..15962
                     /gene="dtpA"
                     /locus_tag="ACHXWD_002156"
     CDS             14460..15962
                     /gene="dtpA"
                     /locus_tag="ACHXWD_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR7621072.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16067..>16181
                     /locus_tag="ACHXWD_002157"
     CDS             16067..>16181
                     /locus_tag="ACHXWD_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR7621073.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
BASE COUNT         3762 a         4116 c         4124 g         4179 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actggcgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc gcgcaagctg gaacagcaac
    10621 aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa agccgccgtc gaagccgcta
    10681 ttgcccgtgc caaagcgcgc aagctggaac agcaacagac taacgcagaa ccagaagaac
    10741 aggttgatcc gcgcaaagcg gcagttgccg cggctattgc ccgcgctcag gccaaaaaag
    10801 ccgcccagca gaaggttgta aacgaggact aaatggtatt cagaatagct agctcccctt
    10861 atacccataa ccagcgccag acatcgcgca ttatgctgtt ggtgttgctc gcagccgtgc
    10921 caggaatcgc agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg
    10981 catcggttag tactctgtta gccgaagctc tcgtactcaa actacgcaag cagtcggtag
    11041 ccgcaacgtt gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc
    11101 cccccctcgc gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta
    11161 aacagttgta tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg
    11221 tcttactgat ctccttcccg gtgcagatga ccagctggtt accgccacat gaaattgcgg
    11281 tcaacatccc tggttttatc gacgccatac aggttatttt cagcgggcat accgccagtg
    11341 gtggtgatat gaacacacta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg
    11401 atacatttaa aacctctgtc cgtgccggtc attcggttga acagattatg caatatccga
    11461 tctacagcgg tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg
    11521 gcggcctgtg gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag
    11581 taacgctggc gttatgcgca acgttgggct ggttgttctc accagaaaca ctggcagcac
    11641 cgcaaattca tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc
    11701 cggttaccgc ttctacgacc aatcgtggtc gtcttatttt cggcgcgctt gcgggcttat
    11761 tagtctggtt gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc
    11821 tggcgaacat cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc
    11881 gcaaagggta aaccatgctg aaaactatcc gaaaacacgg cattacgtta gcgctatttg
    11941 cagcgggttc aacagggtta actgcggcca tcaaccagat gaccaaaacg acgattgctg
    12001 aacaggccag tctgcaacaa aaggcattat ttgatcaggt gctgccagcc gaacgctata
    12061 acaatacgct ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc
    12121 atcgggttta catcgccaaa caggatgaca aaccggtagc cgccgttctg gaagcaaccg
    12181 cgccagatgg ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg
    12241 tacttggcac gcgcgtgaca gagcaccacg aaacgccagg gcttggcgat aaaatcgaac
    12301 tgcgcctttc tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg
    12361 cgcactgggc ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta
    12421 ctccccgcgc ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg
    12481 cacaactttc tcaacttcct gcctgtggag aataaagcgt gagcgaaatt aaagacgtta
    12541 ttgttcaggg gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtcctc
    12601 tgttggcggt cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg
    12661 tactgacgct gaccaacctg accatttcga cgctgcgtca ctggacgcca gccgagatcc
    12721 gcattcccat ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca
    12781 acgcctacgc ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta
    12841 actgtatcgt tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg
    12901 cactggacgg cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac
    12961 tacgcgaaat tatcggcaat ggcacattgt ttgacggtgc agatgcgctg ttaggtagct
    13021 gggcaaaagt attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc
    13081 tgccaccagg tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg
    13141 aaagaatgaa aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa
    13201 cagggaatgt ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa
    13261 caatcctcat cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt
    13321 actgctttcc gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactttaccc
    13381 ggtggcgaat acgcctgcag cgatgcttga actgggcgtt gaaggggtta aaacctatat
    13441 caaaacgatt gggctttata acagcaaagc agaaaatatc atcaaaacct gccgtatctt
    13501 actggagcag cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcccgg
    13561 cgtaggtcgt aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc
    13621 tgtcgacacg cacattttcc gcgtttgtaa tcgtactcaa tttgcgccgg ggaaaaacgt
    13681 cgaacaggta gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca
    13741 ccattggttg atcctgcacg ggcgttatac ctgcattgcc cgcaagcccc gctgtggctc
    13801 ttgtattatt gaagatcttt gtgaatacaa agagaaagtt gacatctgaa gaaaaggggt
    13861 aacaccgatt accccattga taacctttct ttatcctctt ttaaaacatc tttttaacgt
    13921 gaatgatgcc attgcttagc gttatcatca ggtaatccgt ttgcggataa ccaaaaatgc
    13981 aggttaattg tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc
    14041 tgtcgcaatg taaaaacatt gcaagaatca ggttaaaata atttaaattt acacacgcaa
    14101 caaatattga cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta
    14161 gtaaaaacgc taaatagcag aacatatcgc cgttaagcca tcacccccct ctcagtgcag
    14221 tgaaaaaatc tgccattacg ttttttgaaa aatttaacgc tggataacat ttcccggaat
    14281 ggttgaattc cccgcctcag ttatatgtaa cagattatta caaaggactt gtctgaaagt
    14341 gcaagatagt gaacattacc tgccgtttcc cctcccacta taacaattgc gcgtatgttt
    14401 tttagacata acgcgagaaa gcacccccgt taatatggga tgtaaaaaaa gaggtaaaag
    14461 tgtccactgc aaaccaaaaa ccaactgaaa gcgtcagttt gaacgctttc aaacaaccga
    14521 aggcgttcta tctcatcttc tcgattgagt tatgggaacg ttttggttat tacggcctac
    14581 aaggaattat ggctgtttac ctggttaaac aactgggtat gtctgaagcg gattcaatca
    14641 cccttttctc ttcctttagt gccctggttt atggtctggt cgctatcggc ggctggttag
    14701 gtgacaaggt actgggtact aaacgcgtaa ttatgctcgg tgctattgtg ctggcgattg
    14761 gttatgctct ggttgcctgg tctggtcacg acgccggtat cgtttatatg ggtatggcgg
    14821 ctattgcggt cggtaacggc ctgtttaaag ctaacccatc ttctctgctt tctacatgct
    14881 atgagaaaaa cgacccgcgt ctggacggtg cattcaccat gtactacatg tccgtcaaca
    14941 tcggctcttt cttctctatg attgctacgc cgtggctggc cgcgaaatac ggctggagtg
    15001 ttgcgtttgc gttgagcgtt gtaggcctgc tgatcactat cgttaacttc gccttctgcc
    15061 aacgctgggt taaacagtac ggttcaaaac cagacttcga gcctatcaac taccgtaacc
    15121 tgctgctgac cattattggt gttgtggcac tgatcgctat cgccacctgg ctgctgcaca
    15181 atcaggaagt tgcgcgtatg gcgctgggcg ttgttgcctt cggtatcgtg gttatcttcg
    15241 gtaaagaagc cttcgcgatg aaaggtgctg cgcgtcgtaa aatgatcgtt gccttcatcc
    15301 tgatgctcga agccattatc ttcttcgtgc tgtacagcca gatgccaacg tcactgaact
    15361 tctttgcgat tcgtaacgtt gagcactcca ttctgggtct ggccgtagaa cctgagcagt
    15421 atcaggcact gaacccgttc tggatcatca tcggtagtcc gattctggcc gctatctata
    15481 acaagatggg cgataccctg ccgatgccaa ccaagtttgc aatcggcatg gtgatgtgtt
    15541 ctggtgcgtt cctgattctg ccgctgggtg cgaaattcgc gtctgacgct ggtatcgtgt
    15601 ctgtaagctg gctggtcgca agctatggcc tgcagagcat cggggaactg atgatctctg
    15661 gtctgggtct ggcaatggtt gcacaactcg ttccgcagcg tctgatgggc ttcattatgg
    15721 gtagctggtt cctgaccact gccggtgcaa acctgattgg tggttatgtt gcgggtatga
    15781 tggctgtgcc ggataacgtt accgacccgc tgatgtcact ggaagtttat ggtcgcgtat
    15841 tcttgcagat tggtgtcgct actgccatta ttgcagtact gatgctgctg accgcgccta
    15901 aactgcaccg tatgacgcag gatgacgctg cagacaaagc ggcgaaagct gctgtagcgt
    15961 aaaattcagg gaaactcttt tacaagccgc taacttttcg ttagcggctt ttttttgttc
    16021 agcaacgcac taccatactt taaaccacag acaaaaggag ttaccgatga aattgttcta
    16081 caaaccgggt gcctgctctc tcgcttccca tatcaccctg cgtgagagcg gaaaggattt
    16141 taccctcgtc agtgtggatt taatgaaaaa acgtctcgaa a
//
LOCUS       DAZSBF010000086        15963 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZSBF010000086 DAZSBF010000000
VERSION     DAZSBF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.087"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHXWD_002158"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHXWD_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR7621074.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHXWD_002159"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHXWD_002159"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR7621075.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHXWD_002160"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHXWD_002160"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR7621076.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHXWD_002161"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHXWD_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHXWD_002162"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHXWD_002162"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR7621077.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHXWD_002163"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHXWD_002163"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR7621078.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHXWD_002164"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHXWD_002164"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR7621079.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHXWD_002165"
     CDS             8229..8939
                     /locus_tag="ACHXWD_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR7621080.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHXWD_002166"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHXWD_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR7621081.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHXWD_002167"
     CDS             9636..10343
                     /locus_tag="ACHXWD_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR7621082.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHXWD_002168"
     CDS             10343..10891
                     /locus_tag="ACHXWD_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR7621083.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHXWD_002169"
     CDS             10901..12067
                     /locus_tag="ACHXWD_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR7621084.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHXWD_002170"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHXWD_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHXWD_002171"
     CDS             13576..14229
                     /locus_tag="ACHXWD_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR7621085.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHXWD_002172"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHXWD_002172"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR7621086.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15963)
                     /locus_tag="ACHXWD_002173"
     CDS             complement(15612..>15963)
                     /locus_tag="ACHXWD_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR7621087.1"
                     /translation="LFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAA
                     KHQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR
                     VWSGYLTLKSLQRQ"
BASE COUNT         3701 a         4345 c         4163 g         3754 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 aga
//
LOCUS       DAZSBF010000087        15742 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZSBF010000087 DAZSBF010000000
VERSION     DAZSBF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.088"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            118..720
                     /gene="ais"
                     /locus_tag="ACHXWD_002174"
     CDS             118..720
                     /gene="ais"
                     /locus_tag="ACHXWD_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR7621088.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHXWD_002175"
     CDS             complement(759..1184)
                     /gene="nudI"
                     /locus_tag="ACHXWD_002175"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR7621089.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1463..2005
                     /locus_tag="ACHXWD_002176"
     CDS             1463..2005
                     /locus_tag="ACHXWD_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR7621090.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2105..3307
                     /locus_tag="ACHXWD_002177"
     CDS             2105..3307
                     /locus_tag="ACHXWD_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR7621091.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            3527..4309
                     /locus_tag="ACHXWD_002178"
     CDS             3527..4309
                     /locus_tag="ACHXWD_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR7621092.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            4324..5528
                     /gene="rhmD"
                     /locus_tag="ACHXWD_002179"
                     /pseudo
     CDS             4324..5528
                     /gene="rhmD"
                     /locus_tag="ACHXWD_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            5585..6874
                     /locus_tag="ACHXWD_002180"
     CDS             5585..6874
                     /locus_tag="ACHXWD_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7621093.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6892..7695
                     /gene="yfaU"
                     /locus_tag="ACHXWD_002181"
     CDS             6892..7695
                     /gene="yfaU"
                     /locus_tag="ACHXWD_002181"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR7621094.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7736..8638)
                     /locus_tag="ACHXWD_002182"
     CDS             complement(7736..8638)
                     /locus_tag="ACHXWD_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR7621095.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8831..10021)
                     /gene="glpC"
                     /locus_tag="ACHXWD_002183"
     CDS             complement(8831..10021)
                     /gene="glpC"
                     /locus_tag="ACHXWD_002183"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR7621096.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(10018..11277)
                     /gene="glpB"
                     /locus_tag="ACHXWD_002184"
     CDS             complement(10018..11277)
                     /gene="glpB"
                     /locus_tag="ACHXWD_002184"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR7621097.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(11267..12895)
                     /gene="glpA"
                     /locus_tag="ACHXWD_002185"
     CDS             complement(11267..12895)
                     /gene="glpA"
                     /locus_tag="ACHXWD_002185"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR7621098.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            13168..14526
                     /gene="glpT"
                     /locus_tag="ACHXWD_002186"
     CDS             13168..14526
                     /gene="glpT"
                     /locus_tag="ACHXWD_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR7621099.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            14531..15607
                     /gene="glpQ"
                     /locus_tag="ACHXWD_002187"
     CDS             14531..15607
                     /gene="glpQ"
                     /locus_tag="ACHXWD_002187"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR7621100.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(15649..>15742)
                     /locus_tag="ACHXWD_002188"
     CDS             complement(15649..>15742)
                     /locus_tag="ACHXWD_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7621101.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
BASE COUNT         3650 a         3844 c         4248 g         4000 t
ORIGIN      
        1 ggcattttgc cgacatcgta ggatttttaa tattacacca actgctaatt atcgccagaa
       61 atgtcgctca atttcacggt aattgtctgg ttgcgcttgt ctataggtgg agtttacgtg
      121 ttagcttttt gccgctcatc attgaagtca aaaaaatatt tcatcatttt actggcgctc
      181 gctgcaattg ccggactggg tactcatgcc gcctggagta gcaatggttt gccacgtatc
      241 gacaataaaa cattggccag actggcacag cagcacccgg ttgtcgtttt gtttcgccat
      301 gctgaacgtt gcgatcgttc aaccaatcaa tgcttgtcag ataaaacagg tattacggtt
      361 aaaggtaccc aggatgcccg tgaactgggc aacgctttta gcgctgacat ccctgatttc
      421 gatctttatt ccagtaatac cgtccggacc attcagtcgg cgacctggtt ttccgcgggt
      481 aaaaaactga cggtagataa acgacttctt cagtgcggta atgagattta tagtgcaatt
      541 aaggacttac aaagcaaagc gcctgataaa aatatcgtta ttttcaccca taatcattgc
      601 ctgacatata ttgctaaaaa taagcgtgat gcgacattta aacctgatta tctggatggt
      661 ttagtcatgc atgtggaaaa aggcaaagtt tatctggatg gggaattcgt taaccactaa
      721 attttcaatc tgacagccag tgatggctgt catcattgtt aaagaagacc tttcaaacgt
      781 aacgtttttc gggtggcaac atttaaatca taatgcacca gatcttcagg ttttacccac
      841 gcgtagtcct gaaactcttc gtttattttc acttctcggt tggcagaaac gcagtcaaaa
      901 atcaggtaaa tcatataaat ctcttccttg cgaccatctg catacgtctt ggtgcgaata
      961 tcatcgctga aggtccacgg cgtgatttct gtcaaaagca gctgttctcc cagttcttcg
     1021 cgaatttcgc ggcgtagtgc ctcttcaatt cgttcgccag actccacgcc gccacccgaa
     1081 agcgcccatt gaccggggaa aacgccgcga tcgtcggcca ttttgcacag caaataagca
     1141 ccatcatttt gaatcaaagg gcatacaata gtccgttgtc gcacaggggt attccttata
     1201 aataaaaaga aaccatgaga atacggtaac tcagaaatat gtgaaagtga cagtaatctc
     1261 ttttgcgatg ataactgcac ttattattat ttttgtgtgt atcggtaaag tgtgtttatt
     1321 ttttcggcgt ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt
     1381 tcgctcattg aatcctctgt gccaggcagt acaatcccgc agctttggga attttgatta
     1441 attgcttgtg ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct
     1501 gttggtttct gcatcggtca atgcaatgag catcagcggt caggcgggta aagaatacac
     1561 caatattggt gtcggttttg ggactgaatc gacgggcctg gcgttaagcg gtaactggac
     1621 acataacgac gacgacggtg acgtcgctgg cgtggggctg gggttgaatc tgcctctcgg
     1681 gccgttaatg gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga
     1741 aggttatgcc gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt
     1801 gtttggcgag tattactact ctccggattc actctccagc ggtattaaaa gttatgagga
     1861 agcgaatgct ggcgcgcgtt acaccattat gcatccagtc agtattgagg cgggttatcg
     1921 ctacctgaat ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta
     1981 tgttggggtt aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag
     2041 tttcttacta cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat
     2101 aaccatgtta aaagtggaaa tgttatccac cggggatgaa gtgttacacg ggcaaatcgt
     2161 tgacactaac gctgcctggc tggccgattt tttctttcat caggggttgc cattatctcg
     2221 ccgcaatacg gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca
     2281 gcacgccgat gtgctgatcg ttaacggcgg gctgggaccg accagtgatg atttaagtgc
     2341 actcgccgct gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga
     2401 gatggaacgc tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc
     2461 ggagctgcct gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc
     2521 cgtgcggctt aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt
     2581 gatggtcgag cacgaaatcc tgccgcgcct gcgcgagcgt ttttctttac cacagtcgcc
     2641 ggtttgtctg cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga
     2701 cactctacaa ctgccgccgg gcgtaacaat gggctatcgc tcctcaatgc cgatcatcga
     2761 actgaaactc accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt
     2821 taaacgtgtt gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat
     2881 cagtcgcgaa ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg
     2941 tttattggct ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc
     3001 ttcacaggaa gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggccaacca
     3061 ttttgccggg ctggcactgg ctgtttcggg tttcgagaac gagcatctca actttgcgct
     3121 agccacgcca gacggcactt tcgctctgcg tgtgcgtttc agcactacac gctacagcct
     3181 ggctatccgt caggaagtgt gcgcaatgat ggcactgaat atgctgcgcc gttggttaaa
     3241 cggccaggat atcgccagtg agcatggctg gattgaggtt gttgagtcca tgaccttatc
     3301 tgtctgattc cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa
     3361 cttgtccaac ctgttgtact caataagaaa aaatccgtgc actgattcac aaatttgtgc
     3421 gtaatttctc cagtccgctg gacgaggagc gggatttgat gcgcttcaca attttactcc
     3481 ccctgagcaa cgagactgat tccagtatct aagactggag tccagtatgt tggaatcaag
     3541 caaagttccc gccctgacac gggctattga tattctcaat ttgattgccc gcattggccc
     3601 ctgtagtgct gcgacgatca ttgacacatt gggaatccct aaaagcacgg cctatttgct
     3661 gcttaatgaa ctcaggcgtc agcgttttct tagccttgat caccaggaaa atttttgttt
     3721 gtggaccagg ctggtggagc tttctggtca tgcgttaagc aagatggatc tccgggaact
     3781 ggctcgaccg cgtttgacgc aactgatgga taccaccggg ctgttatgtc acctggggat
     3841 tattgataac ggcagcgctt attacatttt aaaagtggag tcatcagcca ctatcagcgt
     3901 gcgttcccat gaaggaaaaa gcctttcgct ttatcgctcc ggaataggta aatgcctgct
     3961 tgcctggcaa cctgcagcgg tacagcaaag tattatcgaa gggctggtct gggagcaggc
     4021 gacacccaca acgattactc atccacagca acttcacgaa gaactggcgc gtattcgccg
     4081 tcagggctgg agctatgaca acggcgaaga ttaccccgat gtccgttgtg tcgctgcgcc
     4141 ggtgtttaac gccaataacg agcttaccgc tgcgatctcg gtggtcggta cccgcttaca
     4201 aatcaatgaa gaataccgtg attatctggc gggcaaggcc attgcctgcg ccagggatat
     4261 ttcacgtctg ttgggatgga aaagtccctt tgacttacag gcctcataac aatggagaac
     4321 atcatgaccc taccaaaaat caaacaggtt cgcgcctggt ttactggcgg tgcgacagca
     4381 gaaaaaggtg ctggcgcggt gattatcacg accagggggc gaatcactgg attgacgatc
     4441 atattgccac cccgatgagt aaataccgcg attacgagca gtcacgccag tcatttggca
     4501 ttaacgttct tggcacgttg attgttgaag tcgaagcaga aaacggccag accggattcg
     4561 ctgtttcgac agccggtgaa atgggctgtt ttattgtcga aaaacatctt aaccgtttca
     4621 ttgagggtaa atgtgtcagt gatatcaaac tgatccatga tcaaatgctc aatgccaccc
     4681 tgtattactc cggctctggt ggcctggtga tgaacacgat ttcctgtgtc gatctggctc
     4741 tgtgggatct gttcggcaaa gtggtcgggc ttccggttta taaactttta ggcggcgcag
     4801 ttcgtgatga gattcagttc tacgccacag gcgcgcgtcc ggatctggca aaagagatgg
     4861 gctttatcgg tggcaaaatg ccgacgcact gggggccaca tgatggcgat gcggggatcc
     4921 gcaaagatgc cgttatggtc gcggatatgc gtgaaaaatg cggtgaggat ttctggttaa
     4981 tgctcgactg ctggatgagt caggacgtga actatgccac caaactggcc cacgcttgcg
     5041 cgccctataa cctgaaatgg atcgaagagt gcctgccgcc acagcagtat gaaggttatc
     5101 gcgaactgaa acgcaacgcg ccagtcggaa tgatggtcac cagcggtgag caccacggca
     5161 cactgcaatc ttttcgtacg ctttcagaaa ccggtatcga cattatgcag ccggatgttg
     5221 gctggtgcgg tggtttaacc acgctggtgg aaattgccgc aatcgccaaa tccagggggc
     5281 aactggtggt gccgcacggt tcgtctgttt actctcacca tgcggtgatc accttcacca
     5341 atacgccatt cagcgaattc ctgatgacca gcccggattg ttcaacgatg cgtccgcagt
     5401 ttgacccgat tctgctcaat gagccggttc cggtgaatgg tcgtattcat aaatcagtgc
     5461 ttgataaacc cggttttggc gtcgaactca atcgtgactg caatttgaaa cgcccctaca
     5521 gccactaatc actccgggcg ttgctgtgac aacggtaacg cccacatatt ctaaggagct
     5581 ttttatgagc accgctttgc ttgacgccgt ggtgaagaaa aaccgtgtgc gtttaattcc
     5641 gtttatgttg gcgctgtatg tgctggcgtt tctcgaccgt tcgaatatcg gttttgccaa
     5701 acagacctac cagattgata ccgggttgag taatgaagct tatgcgctgg gagcaggcat
     5761 tttctttgtg gtatatgcgt ttctgggtgt tccggcgaat cttttgatgc gcaaactggg
     5821 ggccagaacc tggattggta cgacaacact gctgtgggga tttctttcgg cagccatggc
     5881 atgggccgat actgaagcga aatttctgat agttcgcact ctgcttggtg ctgcggaggc
     5941 cggatttttc cctggtatga tttatctcac ctcgcaatgg tttccgcagc gtaatcgcgc
     6001 cagcattatg gggctgttct atatgggcgc accgctggcg ttaacactgg gatcaccgct
     6061 ttccggcgcg ctgttggaga tgcatggatt tatggggcat cccggctggt tctggatgtt
     6121 tgtgattgaa ggattgttgg cagtcggtgc tggggtattc acattctttt ggcttgatga
     6181 cacaccggag caggcacgtt ttctgagtaa acaagaaaaa acgttgctta tcaatcaact
     6241 ggcaagtgaa gaacaacaga aagtgacttc tcggctgagc gatgcgctgc gtaatggccg
     6301 agtctggcaa ctagcgatta tctacctgac cattcaggtg gcggtttacg gattaatttt
     6361 cttcctgccg acccaggttg cggcattgct gggaacaaaa gtgggcttta cagcgtcggt
     6421 ggtcaccgcc attccgtggg ttgcggcctt gtttgggacc tggcttattc cgcgctattc
     6481 cgataaaacc ggcgaacggc gtaatgtcgc agcgctgaca ttactggcgg cgggcattgg
     6541 tattggtctg tccgggctgc tttctccagt aatggcgatc gtagcgctgt gtgttgcagc
     6601 tatcgggttt attgccgtgc agccggtgtt ctggacgatg ccgacacaac tgctttccgg
     6661 tacggcgctg gctgcgggga ttggttttgt aaacctgttt ggtgcagtgg gcgggtttat
     6721 tgccccgatc ctgcgcgtga aagcagaaac gttatttgcc agcgatgcgg caggattact
     6781 gacactggca gcggtggcgg tcatcggttc gctgattatt ttcactctgc gtgtaaatcg
     6841 tactgttgcg cagaccgacg tggcacatca ttaaataggt taaggaacac gatgaacgca
     6901 ttattaacca atccctttaa agaacgttta cgcaagggcg aagtgcaaat tggtctgtgg
     6961 ttaagctcga ctacttctta tatggcagaa attgccgcta cttccggtta tgactggttg
     7021 ctgattgacg gggagcacgc gccaaacacc attcaggatc tttatcacca gctacaggcg
     7081 gtagcgccct atgccagcca acccgtgatc cgtccggtgg aaggcagtaa atcgctgatt
     7141 aaacaagttc tggatattgg cgcgcaaact ctattgatcc cgatggtcga tactgccgat
     7201 caggctcgtc aggtggcgtc tgccacgcgc tatcctccct acggtgagcg tggtgttggg
     7261 gccagtgtgg cacgggctgc gcgctgggga cgcattgaga attacatggc gcaagtcaac
     7321 gattcgcttt gtctgttagt gcaggtggaa agtaaaacgg cactggataa cctggacgag
     7381 atcctcgacg tcgaagggat tgatggcgtg tttattggac cagcggatct ttctgcgtcg
     7441 ttgggctacc cggataacgc cgggcacccg gaagtgcagc gaattattga aaccagcatt
     7501 cggcggatcc gtgctgcggg taaagcggct ggttttctgg ctgtggctcc tgatatggcg
     7561 cagcaatgcc tggcgtgggg agcgaacttt gtcgctgttg gcgttgacac gatgctctac
     7621 agcgatgccc tggatcaacg actggcgatg tttaaatcag gcaaaaatgg gccacgcata
     7681 aaaggtagtt attgatatca aaggcccatg gggatcggct gtgggcctgt gttaattagt
     7741 ggttattcgc tgccagatcg gcttcgctta gctgggtggc cgcgagcacc tggtcacgat
     7801 caatgccctg ttccagcatg cgcaaagcaa ttttaatggc ttgttcatgc atacctttct
     7861 gccagccaat ttggtgtcct tcctgccgta gccgctcagc aatagtcatc aatctctcct
     7921 tttgtagtgg tgaacgttcg gaaatctcct caataaaacg ggtgaaacgg gaggtatcgc
     7981 cgcattgcag cagataatta aacagtgttt gtagctggct gtcattagtg aagcctttaa
     8041 ccaaaagcgt ggtgatcctg tcgaccatac cgattaaatc gcggtcgcga atatgctttt
     8101 gaatcagttc aagcagagct atacgccgat gttgcatgat ctcatcgtca ggtacgatgg
     8161 tgatatccac caacggaaaa gcttcggtgt acaactgccg ggcaagttgc ggatcgtcaa
     8221 actcatccag ccagttaagt gagtacgggt agggcgatgt ttcgccatga taaaacagca
     8281 atggcaccac cagcggaact ctgtcatagc ctttatccag gtgacgctgc atggcggcag
     8341 tggcatagcg cattagccga aaagccatat tcttttctgc agagctttga tgttcaatca
     8401 cgcagtagat atagccgtca ccgtcgctgg tttccacgga ccacaaaaca tccgagtagt
     8461 aagcgcgtaa acttttttca ataaaactag tgggttccag gcgtaaggtt tgcaggttgc
     8521 aaagcttgcg cagtggttct ggcaaatgta tttcgagaaa atcccgtgcg gtttcgggtg
     8581 tgaacataaa ggttttaaat accgcatcat gcggggagga ggttgttgat tcggtcatgg
     8641 ttgtccgtca cctgatattt atgatgacgc gacattacgg ttatcccagg cctttgacat
     8701 ctgccagttt cagcctttgc gagacgtttt cagaggaatt ttcactgcgt tgcaacgcat
     8761 acattgggca cggaatcgtg tacgcaaaaa tgcccggtaa ccacatgctt accgggcatc
     8821 agaaaggagt ttaagccagc gcctgggcca gtagcgtaat cggatgttcg cagcgaagac
     8881 ttgtggacat ctcaatctgc catttacagg tttcgcagtc ggtgaccacc agatctgcgc
     8941 cgctttcttc tatctggcgg aacagtggtg cgccgatggc ttgtgaggtg gggtagttct
     9001 cttttttgaa accgtaagta cccgcaatac cgcagcactg ggaatccagc accgttaact
     9061 caagccccgg gattttacgc aacagctcca gggtgtagag cgtccagccc attttttcca
     9121 tatggcacgg agtgtgataa accactttca gcggcagcgg tttcagcggt aacgttttgc
     9181 cttcgtccag cttgcgccac agccagcggg ttgccagttc gatatgatcg cgcaagcctt
     9241 tgttgtcgac attcagcact tccgggtatt cgtcgcgcag ggcaaatgta caggttgagg
     9301 aggtggcaat caccggaatg ccttttactc ccacagcttc gcggatcgac tctacattcg
     9361 taattgcctg tttgcgtgct ttatcggtaa agccgttggc gattagcggt acgccgcagc
     9421 atttttcttt gctgagcagt tgtacaccga tacccattgc gttgagcact ttaattaaat
     9481 ctttacctaa ctgcggatgg ttgtagttaa cgaagcagcc gtgaaagaaa gcgacctggt
     9541 ctttatattg tgcttgctga gccgctatgc tgcgatacca gcgacggaac gtgccgaagg
     9601 agtatttcgg tagcgtgcga cgatgatcga tttttaacgc cgcatcaagc agctgccgca
     9661 ccggtttcag cgaggtagcg gtgttgacga ttggtgcgaa cggcgtggaa acgctaccca
     9721 tcaggtcggt atgactcaac acaaaattac gcagcgacgg gcgcgtggtg tcatattttg
     9781 cccgcgcgcg ctggataata tcgccaatct tcacatcgga cgggcaggcg acttcacaac
     9841 gtttgcagtt gatgcaatat ttcagcgcct cgtcatacag tgcgccatct ttcaaacgca
     9901 gacgctcgcc atccggcccg gcttgttttg gccctggata accgggattc acccggctca
     9961 ccgggcaggc ggtggtgcag acggtgcact taatgcagtt ttcgaagctg gtgtcgttca
    10021 ttgttggcct cctgcgcgtt gggcaatctg ttgagcggca tgtaaagcac tgacggcaca
    10081 aacaccgccg ccgcatcctt gggcgatggg atcaaatccg cccagcaccg aaccgatggc
    10141 aaacaggttc tcaatggttt gccctgcctg cgacgggcgt agcgtctcat cagtggttac
    10201 accgaactgc tgccacggtt gcggcgcaaa aaaatcgccc ttataccatt caccccgcgt
    10261 ggcggtttgt agcacatcaa ggccgagaat cggctcgcga atgccgttac gttccgctac
    10321 cagtccgcca ctaaagaaac tgccgctggc gagaaccgcg aaacgtggac gtagcggaat
    10381 atcggcgtga ttgcgggtcc agatttcatt cactacgcca tttttacagg tcactttttt
    10441 cacttcatcg cccggcatcc acacgccacc ctggcgcaca aactggcgct gtaactggtt
    10501 ttgcagacga atgcccagca cggaaggcgg cagcgttggc aaaagcatca gtgaacaagg
    10561 tagtttttca ttcaaccaac gccacagttt gtcatcggcc aggccgaagc aggcgggcat
    10621 caggatcatt tcgcaggtat tggcgacagg aataagcgca tcaagtaaca gcggccagtt
    10681 ttcttcatta tcaaggaaac gggcgatatt caccgcgcga aattcggtgg cgttattgcg
    10741 caggacatcc agttccggca gctctatttc tgcggtttca accgaaagat ccagttcgcg
    10801 caacgaagcc gccgcaagat gcgcctggaa atccatcagc ccgcttatcc ccactacaca
    10861 tatcttcttc gcgggtagcg gccagacggg aacttctggc gaacttaacc aggttgcacg
    10921 caaagtgccg agcggggtaa cgcgctgatg ggccagttcc acgctgccct gcaattgcgc
    10981 accgctttca gcgatcaatg cctgcgcctg gcaagcgaga tcgagcacgc gttgtggttc
    11041 gagaagactg taaggatggg ctggtgcctg ctgacgcaaa gattccagtc cactgtgaat
    11101 gtctgtcacc ggttgaccat caggcagatg gctcagcaaa tccagcgaac cggatgagaa
    11161 atgcagcgcg ctttgaccac gcgtgacaat ggcacagcgc aggccgtgtt tttgcagttg
    11221 caggccacag agtaatccgg cgaggccgcc gcccataatg acagtatcaa agcgcatctt
    11281 tctgctcctt ctccagacca cacagtccct gataaaccca gcgggtaaat tcgctttcgc
    11341 gcagtgcatc tccccaggcg atgggttgca cgcctttcca gcgttcgtta aggaaggtgg
    11401 aaagttgctc gatagattgc gcggacgtcg tgacgttaaa acgttgcagc agtccggcag
    11461 cgcggcaggc gcagagttcg ccctggcagg tacccatccc cacacgggta cgacggcgta
    11521 aatccagcag gctattaacg tttaaatttt ctaccgcgta ctgcacttca cccgctgtta
    11581 ccgcttcgca ctcacatacc aggctacggt gcagacgacc ttcgctcagc caggcaggcg
    11641 tgcgatcgcc atgacgataa accgcagaac cgcgcagcgg ggcaggcagg gagatgactt
    11701 tacgcaaggt aacttcagcg ggttcctgtg aaccaggaag tgccagatcg gcggtcgtac
    11761 aggggcgcgt gttgcccagt ttgcggcaca cggcgtcggt agcccattca gccatcagcc
    11821 gataggtcat cagtttgcca ccggtgatgg tgataaatcc gtccagacca tcgcgttcag
    11881 catggtcgag cagcacgatg ccacggctga cgttacgtcc gctcgggtcg tcatcgctgg
    11941 caaccagcgg gcgcacgcca gaataggccc gcaaaatgcg cgttttcgcc atcacggggg
    12001 ccagtttttc cccttcacgc agcagaatat caacctcttc tgccgtcact cgattatcgt
    12061 caatctcgtt gtaatcaata cgtaaagagg tggtgccaat cagcgaaatg gtatcgccag
    12121 gcaccagaat atcggcgtcg gaaggtttac ggcagcggtt gatcacatgc tggttaatgc
    12181 ggtgatccat gatcagcagc gatcctttcg ccgggaacat gcgaatgcgc agatcggcat
    12241 attcggcaat gtgttgcccc cagatcccgg cggcattaac cacgacaggc gcatgaaggg
    12301 cctgagtttc gccggtgaga tggttacgta cacgaacacc gcacaccgtc gcgccttcac
    12361 gaatcagtcc cgtgacttca tgagcggtaa ggataacggc accgtgttct ttggcatcca
    12421 gcatgtttgc tgcggtcaga cgaaatggat caacggtgcc atccggaact ttcaccgcgc
    12481 caatcaatgc cgggttaacg gcaggttcga taatgcgcgc ttgctgcggg tctatagctt
    12541 ctgcgctgat ccctgcttct tcgcaggcgc gaataaaagt ggcctggaag gagaggtcat
    12601 cttccggcag ggtaataaac aggccgttgg ttggttcaac gcagtgacgt gcaatgcgtt
    12661 tcaggatctg gttttcacta atgcattcgc gggccgattc cgcatcggtt accgcatagc
    12721 gcgcaccgct gtgtagcagg ccgtggttac gcccggtggc accggttgcg atgtcgtggc
    12781 gctcaaccaa aatcacgcgc agcccgcgca gggcacagtc gcgggcaatc ccggctcccg
    12841 ttgcgccgcc gccaatgata atcacgtcac ttgattgcga gtcgcgagtt ttcattgtta
    12901 tccctctgaa gttcgttttt taccatttag ccatagtaaa aacatgaatt gtttgatttc
    12961 gcgcatattc gctcatgatt cgaaagtgaa gcgtgatttc atgcgtcatt ttgaacattt
    13021 tgtaaatctt atttaataat gtgtgcggca attcacattt aatttatgaa tgttttctta
    13081 acatcgcggc aactcaagaa acggcaggtt ctctcactga atcaggctgt taatcataaa
    13141 taagaccacg ggccacggag gctatcaatg ttgagtattt ttaaaccagc gccacacaaa
    13201 gcgcgcttac ctgccgcgga gatcgatccg acttatcgtc gattgcgctg gcaaattttc
    13261 ctggggatat tctttggcta tgcggcttac tatttggttc gtaagaactt tgcgcttgct
    13321 atgccttatc tggttgagca gggattctca cgcggtgatt taggttttgc cctttcgggg
    13381 atctcgattg cttatggatt ttcgaaattc atcatgggtt cggtatcgga tcgctcgaat
    13441 ccgcgcgttt tcctgcccgc aggtttgatt ctggcggcgg cagtgatgtt gtttatgggc
    13501 tttgtgccat gggcgacgtc gagcattgcg gtgatgtttg tactgttgtt cctctgcggt
    13561 tggttccagg ggatggggtg gccgccgtgt ggtcgtacta tggtgcactg gtggtcgcag
    13621 aaagaacgtg gcggcattgt gtcagtgtgg aactgtgcgc acaacgtcgg tggtggtatt
    13681 ccgccgctgc tgttcctgct ggggatggcc tggttcaatg actggcatgc ggcgctctat
    13741 atgcctgctt tctgcgccat tctggtggca ttattcgcct ttgcgatgat gcgcgatacc
    13801 ccgcaatcct gtggcttgcc gccgatcgaa gagtacaaaa atgattatcc ggacgactat
    13861 aacgaaaaag cggaacagga gctgacggcg aagcaaatct tcatgcagta cgtactgccg
    13921 aacaaactgc tgtggtatat cgccatcgcc aacgtgttcg tttatctgct gcgttacggc
    13981 atcctcgact ggtcaccgac ttatctgaaa gaggttaagc atttcgcgct ggataaatcc
    14041 tcctgggcct acttccttta tgaatatgca ggtattccgg gcactctgct gtgcggctgg
    14101 atgtcggata aagtcttccg tggcaaccgt ggggcaaccg gcgttttctt tatgacactg
    14161 gtgaccatcg cgactatcgt ttactggatg aacccggcag gtaacccaac cgtcgatatg
    14221 atttgtatga ttgttatcgg cttcctgatc tacggtcctg tgatgctgat cggtctgcat
    14281 gcgctggaac tggcaccgaa aaaagcggca ggtacggcag cgggctttac cgggctgttt
    14341 ggttacctgg gcggttcggt ggcggcgagc gcgattgttg gctacaccgt ggacttcttc
    14401 ggctgggatg gcggctttat ggtaatgatt ggcggcagca ttctggcggt tatcttgttg
    14461 attgttgtga tgattggcga aaaacgtcgc catgaacaat tactgcaaaa acgcaacgga
    14521 ggctaatggc atgaaattga agctgaaaaa ccttagcatg gcgatcatga tgagcactat
    14581 agtcatggga agcagtgcaa tggcggcgga cagcaacgaa aaaatagtca tcgcccatcg
    14641 cggtgccagt ggatatttgc cggagcatac gctgccagca aaagcgatgg cgtatgcgca
    14701 gggagcggat tatctggaac aggatttggt gatgaccaaa gacgaccatc tggttgttct
    14761 gcatgaccat tatctcgatc gtgttactga tgttgccgat cgtttcccgg atcgggcgcg
    14821 caaagacggt cgttactacg cgatagattt cacgctggat gaaattaagt cgctgaaatt
    14881 taccgaaggt ttcgatattg aaaacggtaa aaaagtacag acttatccgg ggcgtttccc
    14941 aatgggtaag tccgacttcc gggggcacac ctttgaagaa gagattgaat ttgttcaggg
    15001 gttaaatcac tctaccggga aaaatatcgg tatctatcca gaaatcaaag cgccgtggtt
    15061 ccatcatcag gaagggaagg atattgcggc aaaaacgctg gaagtgctga agaaatatgg
    15121 ttacaccggt aaggacgata aagtttattt gcaatgtttt gatgctgatg agctgaagcg
    15181 tattaagaat gagctggaac ctaaaatggg catggatctc aatctggtac agctgattgc
    15241 ctataccgac tggaatgaaa cgcagcagaa acagccggac ggaagctggg ttaattacag
    15301 ctatgactgg atgtttaagc caggtgccat gaagcaagtc gcggaatatg ccgacggtat
    15361 cgggccggat taccatatgt tgattgagga aacatcgcag ccgggtaata tcaaactcac
    15421 tggcatggtg caagatgctc agcagaataa actggtagtg catccttata ccgtgcggtc
    15481 ggataaactg cctgaataca ctactgatgt gaatcagtta tatgatgctc tgtataacaa
    15541 agcgggtgta aatgggctgt ttactgattt ccctgataag gcagtaaaat tccttaataa
    15601 agagtaatgc tactgcagga tggcgttcat gcgccatcct gtctcatatc aaatttttcc
    15661 aatcgccact aattgttcca tgcacatttg tagtaaaaac agtggactgc cttctaccgg
    15721 cttttcttta gccaacaacg aa
//
LOCUS       DAZSBF010000088        15618 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZSBF010000088 DAZSBF010000000
VERSION     DAZSBF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.089"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(16..102)
                     /locus_tag="ACHXWD_002189"
     tRNA            complement(16..102)
                     /locus_tag="ACHXWD_002189"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66..68),aa:Leu,seq:cag)
     gene            complement(131..217)
                     /locus_tag="ACHXWD_002190"
     tRNA            complement(131..217)
                     /locus_tag="ACHXWD_002190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Leu,seq:cag)
     gene            complement(485..1516)
                     /gene="rsmC"
                     /locus_tag="ACHXWD_002191"
     CDS             complement(485..1516)
                     /gene="rsmC"
                     /locus_tag="ACHXWD_002191"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR7621102.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1619..2032
                     /gene="holD"
                     /locus_tag="ACHXWD_002192"
     CDS             1619..2032
                     /gene="holD"
                     /locus_tag="ACHXWD_002192"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR7621103.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            2001..2447
                     /gene="rimI"
                     /locus_tag="ACHXWD_002193"
     CDS             2001..2447
                     /gene="rimI"
                     /locus_tag="ACHXWD_002193"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR7621104.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2462..3139
                     /gene="yjjG"
                     /locus_tag="ACHXWD_002194"
     CDS             2462..3139
                     /gene="yjjG"
                     /locus_tag="ACHXWD_002194"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR7621105.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3230..4819
                     /gene="prfC"
                     /locus_tag="ACHXWD_002195"
     CDS             3230..4819
                     /gene="prfC"
                     /locus_tag="ACHXWD_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR7621106.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5212..5817
                     /gene="osmY"
                     /locus_tag="ACHXWD_002196"
     CDS             5212..5817
                     /gene="osmY"
                     /locus_tag="ACHXWD_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR7621107.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5944..6105
                     /locus_tag="ACHXWD_002197"
     CDS             5944..6105
                     /locus_tag="ACHXWD_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR7621108.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6227..7300
                     /locus_tag="ACHXWD_002198"
     CDS             6227..7300
                     /locus_tag="ACHXWD_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR7621109.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7297..8079
                     /locus_tag="ACHXWD_002199"
     CDS             7297..8079
                     /locus_tag="ACHXWD_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7621110.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8595..9458)
                     /locus_tag="ACHXWD_002200"
     CDS             complement(8595..9458)
                     /locus_tag="ACHXWD_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR7621111.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(9430..10980)
                     /locus_tag="ACHXWD_002201"
     CDS             complement(9430..10980)
                     /locus_tag="ACHXWD_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR7621112.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            11238..12017
                     /gene="deoC"
                     /locus_tag="ACHXWD_002202"
     CDS             11238..12017
                     /gene="deoC"
                     /locus_tag="ACHXWD_002202"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR7621113.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            12144..13466
                     /gene="deoA"
                     /locus_tag="ACHXWD_002203"
     CDS             12144..13466
                     /gene="deoA"
                     /locus_tag="ACHXWD_002203"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR7621114.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13518..14741
                     /gene="deoB"
                     /locus_tag="ACHXWD_002204"
     CDS             13518..14741
                     /gene="deoB"
                     /locus_tag="ACHXWD_002204"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR7621115.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14821..15540
                     /gene="deoD"
                     /locus_tag="ACHXWD_002205"
     CDS             14821..15540
                     /gene="deoD"
                     /locus_tag="ACHXWD_002205"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR7621116.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3682 a         4094 c         4243 g         3599 t
ORIGIN      
        1 tgatatcgcc tcgtttggtg cgagggggga gacttgaacc cccacgtccg taagaacact
       61 aacacctgaa gctagcgcgt ctaccaattc cgccaccttc gcccagtgcg agcaatatca
      121 acgtggtttt tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac
      181 ctgaagctag cgcgtctacc aattccgcca ccttcgcata ccatcaattc ttaaaaagaa
      241 ttgctaccac ggaggcgcat tctagtggtt ttcagctttt cgtcaatagt taattatcgc
      301 cggaggtgta attgctggaa aaatgtccat caggaaacta gcgtgcaggt ttggtaggca
      361 tgcgggggca gatgccagat gcgacgctgg cgcgtcttat ctggcctacg aagggctaac
      421 gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat ccgacacggt attcggcgag
      481 ataattaacc tttcttcgcc tggcgggtca taatggcgcg atacaccttg aaacgcccgg
      541 tttgcgcaat cacttcatgg aagccaaatg tctcatccag cacgtccggg taaggcagga
      601 aggcgttcgc tacaattcgc agctcgccgc cgctattaag atgacgcacc gcgccgcgaa
      661 tcagcgtttg cgccgcatcc aggctggttt gcatcccatc gtggaacggc gggttggaga
      721 tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt gctggcaaag acttcacctt
      781 caacacagtt ggccgcaagt gttgcgcggc tggcttctac cgctggcgca gagacatcgc
      841 acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc aactgaaagc acccccgcgc
      901 cacagccgac atccagcact ttacctttcg tgtgcggagt taacgtcgag agcagcaact
      961 ggctaccgac atccagaccg tcgcggctaa acacgccagg cagcgttttg accgtcaggc
     1021 catcgacgct gtattcgccc cagaatttat cggcatcaaa taccggctgt ttttccagac
     1081 gaccaaaata gaggccacag cgacgagcgc tgtcgacttt attcaacggc gcataatctg
     1141 ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc gccaacgaca aaaatatctg
     1201 tccccactgg cagcagagaa agtaaattca tcaactggaa ctgggcttcc ggtttgttct
     1261 tcggccagta gtaaatcagc atatcgcaat ctgcgacgtc atccaccgtg gcgaccagac
     1321 tgaaacgggc gttatccccc atctggcggc ttaatacctg ccagtggtgg aattgctggg
     1381 tatgagcacg gctggccgcg gtatctaaac gcgcgggcag gtcatcctgt aagtctccgg
     1441 caaacagaat acggctttgt tcgaaatcat cactgtgacg cagcaagact tcacttgccg
     1501 gggtaaatgc agacatggaa tgctcctcaa ttgatactgg cggcgattat agccatatgt
     1561 tggcgcggta tcgacgaatt tgctatattt gcgcccctga caacaggagc gattcgctat
     1621 gacatcccga cgagactggc agttacagca actgggcatt acccagtggt cgctgcgtcg
     1681 ccctggcgcg ttgcaggggg agattgccat tgcgatcccg gcacacgtcc gtctggtgat
     1741 ggtggcaaac gatcttcccg ccctgaccga tcctttagtg agcgatgttc tgcgcgcatt
     1801 aaccgtcagc cccgatcaag tgctgcaact gacgccagaa aaaatcgcga tgctgccgca
     1861 aggcagtcgc tgcaacagct ggcggttggg tactgacgaa ccgctatcac tggaaggcgc
     1921 tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac ccaacggcac gcgccgcgtt
     1981 atggcaacag atttgcacat atgaacacga tttcttccct cgaaacgact gatttaccag
     2041 cggcttacca cattgaacaa cgcgcccacg cctttccgtg gagtgaaaaa acctttgcca
     2101 gcaaccaggg cgagcgttat ctcaactttc agttaacgca aaacggcaaa atggcggcgt
     2161 ttgcgattac gcaagtggtg ctggatgaag ctacattgtt caatattgcg gtcgatcctg
     2221 actatcagcg tcagggattg ggaagggtgc tgctggaaca tctgatcgac gaactggaaa
     2281 aacgcggcgt ggcgacacta tggctggaag tccgtgcctc aaatgctgcc gccattgccc
     2341 tgtacgaaag tttaggcttt aacgaggcga cgattcgccg caattactac cccaccacgg
     2401 acggtcgcga agacgccatc atcatggcgt tgccaatcag tatgtaagac aaggtggaat
     2461 aatgaagtgg gactggattt tctttgatgc cgatgaaacg ctgtttacct ttgactcatt
     2521 caccggcctg cagcggatgt ttcttgatta cagcgtcacc tttaccgctg aagattttca
     2581 ggactatcag gccgttaaca agccactgtg ggtggattat caaaacggcg cgatcacttc
     2641 attacagctt cagcacgggc gttttgagag ctgggccgaa cggctgaaag ttgaagcagg
     2701 cttgcttaac gatgccttta ttaatgcgat ggcggaaatc tgcacgccgc tgccgggcgc
     2761 ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc ggcatcatca ccaacggctt
     2821 tagcgccttg cagcaagtgc gtctggaacg cacgggcctg cgtgattact tcgatttgct
     2881 ggtgatttcc gaagaagttg gcgttgccaa accgaataag aaaattttcg attatgcgct
     2941 ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg gttggcgaca ctgccgagtc
     3001 cgatattctc ggtggcatca acgccgggct tgcgacctgc tggctgaatg cgcacaatcg
     3061 cgagcaacca gaaggcatcg cgcccacctg gaccgtttct tcgttgcacg aactggagca
     3121 gctcctgtgt aaacactgat tgcctccccc ccgttgatgg gtaaaatagc cgcaattttt
     3181 cgttttcaac aagcgcggcg cgatgccgct tactcaagaa gaaagaatta tgacgttgtc
     3241 tccttatttg caagaggtgg cgaagcgccg cacttttgcc attatttctc acccggatgc
     3301 cggtaagact accatcaccg agaaggtgct gctgttcgga caggccattc agaccgccgg
     3361 tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg gactggatgg agatggaaaa
     3421 gcagcgtggg atctccatta ctacgtctgt gatgcagttt ccgtatcacg attgcctggt
     3481 taacctgctc gacaccccgg ggcacgaaga cttctcggaa gatacctatc gtaccctgac
     3541 ggcggtggac tgctgcctga tggttatcga cgctgcaaaa ggtgttgaag atcgtacccg
     3601 taagctgatg gaagttaccc gtctgcgcga cacgccgatc ctcaccttta tgaacaaact
     3661 tgaccgtgat atccgcgacc cgatggaact gctcgatgaa gttgagaacg agctgaaaat
     3721 cggctgcgca ccgatcacct ggccgattgg ctgcggcaag ctgtttaaag gcgtttacca
     3781 cctttataaa gacgaaacct atctctatca gagcggtaaa ggccacacga ttcaggaagt
     3841 ccgtattgtt aaagggctga ataacccgga tctcgacgct gcggttggtg aagatctggc
     3901 acagcagcta cgtgacgaac tggaactggt gaaaggcgcg tctaacgagt tcgacaaaga
     3961 attgttcctt gcgggcgaaa tcactccggt attcttcggt actgcgctgg gtaacttcgg
     4021 cgttgatcat atgctggatg gcctggtgga gtgggctcct gcgccgatgc cgcgtcagac
     4081 tgatacccgt accgtagagg cgagcgaaga caaatttacc ggcttcgtat ttaaaattca
     4141 ggccaacatg gacccgaaac accgcgaccg cgtggcgttt atgcgcgtag tatccggtaa
     4201 atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg aaagatgtgg ttatctccga
     4261 cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa gaagcgtatc cgggcgatat
     4321 cctcggcctg cacaaccacg gcactattca gatcggcgac acctttaccc agggtgagat
     4381 gatgaagttc accggtattc cgaacttcgc accagaactg ttccgtcgta tccgcctgaa
     4441 agatccgctg aagcaaaaac agctgctcaa agggctggta cagctttccg aagagggcgc
     4501 ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc gttggtgctg ttggtgtgct
     4561 gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac agcgttgaag cagtatatga
     4621 atcagtcaac gttgccactg cccgctgggt agaatgtgcg gacgcgaaga aattcgaaga
     4681 gttcaagcgt aagaacgaaa gccaactggc gcttgatggc ggcgataacc tcgcttacat
     4741 cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt tatcccgacg ttcagttcca
     4801 ccagacccgc gagcattaat tcttgcctcc agggcgcggt agccgctgcg ccctgtcaat
     4861 ttcccttcct tattagccgc ttacggaatg ttcttaaaac attcactttt gcttatgttt
     4921 tcgctgatat cccgagcggt ttcaaaattg tgatctatat ttaacaaagt aatgacattt
     4981 ctgacggcgt taaataccgt tcaatgcgta gatatcagta tctaaagccg tcgattgtca
     5041 ttctaccgat attaataact gattcagagg ctgtaatggt cgttattcat cacccatcgc
     5101 ttttgtgatg gcgaccattg acttctgtag agggtgaagt ctctccctat tcagcaatgc
     5161 aacctcgtgt tgccaggctc aaattacgag caaacataca ggaataaatc gatgactatg
     5221 acaagactga agatttcgaa aactctgctg gctgtaatgt tgacctctgc cgtcgcgacc
     5281 ggctctgcct acgcggaaaa caacgcacag actaccaatg aaagcgcagg gcaaaaagtc
     5341 gatagctcta tgaataaagt cggtaatttc atggatgaca gcgccatcac cgcgaaagtg
     5401 aaggcggcgc tggtggatca tgacaacatc aagagcaccg atatctctgt caaaaccgat
     5461 caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg ctcaggccga agaggcagtg
     5521 aaagtggcga aaggcgttga aggggtgacc tctgtcagcg acaaactgca cgttcgcgac
     5581 gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg ccaccaccag tgaaatcaaa
     5641 gccaaactgc tggcggacga tatcgtccct tcccgtcatg tgaaagttga aaccaccgac
     5701 ggcgtggttc agctctccgg taccgtcgat tcccaggcac agagcgaccg tgctgaaagt
     5761 atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg atctgaaaac taagtaattc
     5821 gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat gtgcaccaca ctaaaaatat
     5881 cgcattgagt gattttcacg ctcatatcaa gcggtgacat taactatggt aaaggagacg
     5941 cttatgtttc gttggggcat catatttctg gttatcgcgt taatcgccgc cgcacttggg
     6001 tttggtggtc tggccggtac cgctgcaggc gcagctaaaa ttgtctttgt cgtcgggatt
     6061 attctgttcc tggtgagttt gttcatgggc cgaaaacgac cctagatttc agaccattaa
     6121 gaaaatttta tccaaagcca gtccagcgga ctggcttttg cggttttagc gaataataaa
     6181 ttgcgttact ttgtcattct ttcacaacaa aaacaggaag gcagaggtgg ggcagcgaat
     6241 acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg ccgttccagc ctggacgaat
     6301 agctctggtg tgcgaaggcg gcggacagcg tggaattttc acggctggcg tgctggatga
     6361 gtttatgcgc gcgcagttta atcctttcga tctttatctc ggcacatctg ccggggcgca
     6421 gaacctctcg gcgtttatct gcaatcagcc cggttacgcg cgcaaagtca tcatgcgcta
     6481 taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc cgtggaggaa atcttatcga
     6541 tctcgactgg ctggtggagg ccaccgcaag ccagatgccg ttgcaaatgg ataccgccgc
     6601 gcggttgttt gacagcggca aatcgtttta tatgtgcgcc tgtcgtcagg atgactacgc
     6661 gcctaattac tttttaccaa ccaaacaaaa ctggctggat gtgattcgcg cctccagtgc
     6721 gatacctggc ttttatcgta gcggagtgtc gctggaaggc attaactacc tggatggcgg
     6781 gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag ggcgctaaaa cgttggtcgt
     6841 cattcgcact gtgccgtcac aaatgtacta cacgccgcag tggttcaaac gcatggaacg
     6901 ctggctgggt gacagtagcc tgcagccgct ggtcaatctg gtgcagcatc atgaaaccag
     6961 ctatcgtgac attcagcaat ttattgagaa accaccgggc aagctgcgga tattcgaaat
     7021 ttatccgccg aagccattac atagtatcgc gcttggcagt cggattccgg cgctgcgtga
     7081 agactataaa cttgggcgtt tatgcggtcg ttatttcctc gccacggttg gcaagctatt
     7141 aactgaaaaa gcgccgctta cccgccatct ggtgccagtg gtgacgccgg aatcgatcgt
     7201 tattccgcct gcgccagtcg ccaacgatac gctggttgcc gaagtgagcg atgctccgca
     7261 ggcgaacgac ccgacattta acaatgagga tctggcttga tttgccgttt tatcgatacc
     7321 cactgccatt ttgatttccc gccgtttagt ggcgatgaag aggccagcct gcaacgcgcg
     7381 gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag aggcggaaaa ttttgcccgc
     7441 gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg cattggggtt gcatccgggg
     7501 atgttggaaa aacatagcga tgtgtctctt gatcagctac agcaggcgct ggaaaggcgt
     7561 ccggcgaagg tggtggcggt gggggagatc ggtctggatc tcttcggcga cgatccgcaa
     7621 tttgagaggc agcagtggtt actcgacgaa cagctgaaac tggcgaaacg ctacgatctg
     7681 ccggtgatcc ttcattcccg gcgtatacac gacaaactgg cgatgcatct taaacgtcac
     7741 gatttaccgt gcactggcgt ggttcacggt ttttccggca gcctgcaaca ggccgagcgg
     7801 tttgtacagc tgggctacaa aatcggcgta ggcggtacta tcacctatcc acgcgccagt
     7861 aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt tgttgctgga aaccgacgcg
     7921 ccggatatgc cgctcaacgg ctttcagggg cagcctaacc gaccggaaca ggctgtgcgg
     7981 gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag atgagattgc cgaagtgttg
     8041 cttaataata cgtatgcgtt gtttagcgtt agtgggtagc ctacccttcg taggcagcat
     8101 ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc gtcttatcat gcctacaggt
     8161 ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca tccggcagtt ggcgcacaat
     8221 gcctgatgcg acgcttgacg cgtcttatca tgcctactgg tttgtgccga accgtaggcc
     8281 ggataaggcg ttcacgccgc atccggcagt tggcgcacaa tgcctgatgc gacgcttgac
     8341 gcgtcttatc atgcctacaa gtctgtgccg aaccgtaggc cggataaggc gttcacgccg
     8401 catccggcag ttggcgcaca atgcctgatg cgacgctgac gcgtcttatc atgcctacaa
     8461 gtctgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag tcggcgcata
     8521 atgcctgatg cgacgcttgt cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg
     8581 ccgtatccgg catgtcacaa atagagcgcc ggaaatatca accggctcac cccgcgcacc
     8641 tttaacgcat cagccaacgg ctcaacgtct tccggcgtgg cgctcgccca gctttgcgcc
     8701 tcgccataca cgccgtgggc atgaaacgca ttcaggcgaa ccggaacatc gccaagtctc
     8761 ttgataaacg tcgccagctc atcgatgtga tgcaaataat ccacctggtc tggaattacc
     8821 agcaaacgca gttccgccag cttgccgcgc tctgcaagca aacagatgct gcgcttaatc
     8881 tgctgattat cgcgtccggt taagtgttga tgacattcgc tcccccacgc tttgagatcg
     8941 agcattgcgc cgtcacacac cgggagtaat ttttcccagc cggtttcgct caacatgccg
     9001 ttactgtcca ccaggcaggt gaggtggcgc agttgcggat cgtttttgat agcagtaaac
     9061 agcgccacta caaacggcag ttgggtcgtg gcttcgccgc cgcttaccgt tatcccttca
     9121 ataaacagca ccgctttgcg gacatggcta agcacttcgt ccacgctcat ggattgcgcc
     9181 attggcgtgg catgttgcgg acacctcttc aggcaggtat cacactgctc gcaaaccaca
     9241 gcgttccaca ccactttgcc gtcaacaatc tgcaacgcct gatgcggaca ctgtggcacg
     9301 cactccccac agtcattgca acgtcccatc gtccacggat tgtgacagtt tttgcagcgc
     9361 agattgcagc cctgcaaaaa cagagccaga cgactgcctg gcccgtcaac gcaggagaag
     9421 gggataatct tactgactaa agcgcatctg ctgttcatgg cttatcacgc gcggctggcg
     9481 ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc caggtggtgt tggtgcgtga
     9541 accttcggcg cgatattttt ctaaatccga caaacgcacc atataaccgg taacgcgaac
     9601 cagatcgtta ccgctgacat tggcagtaaa ttcacgcatt ccggctttaa aggcaccgag
     9661 gcaaagctgt accagcgcct gcggattacg tttgatggtt tcgtcgagcg tcagaatgtc
     9721 gctgatgccg gaataataat aagcatgatg cggggcgaca gtttgcagat gggtgatcgg
     9781 atctggctca tcaccatacg gcaaacgcgc gcccggcgtg gtgccgatat cggaactgct
     9841 ccccgactgt gcgtgtaaca tggcgcgttt ttgccagcca tatttcacgg gggtattggc
     9901 gacaaactcc gccagttgcg cgctgatgcg ataacccact tcatttgcgg cggcttcttt
     9961 accgtagcgc gcggcaatcc cttctttctt acacagcaag ttaaccgctt ccgccagccc
    10021 atacatgcca aacattggca caaaacgttc agggttaatc agcccttctt tcaccaggaa
    10081 gctattctca aagaagtgtg attgttgata gaggaattca caccgcgcat cgatgatggc
    10141 gatctgctgc tggcagtagt gcggtagagt gcgcgtaaag aagtcatcca gcgattcgct
    10201 gcgctcggca atggctttca ggttaaggcg taccagcgtg ctgccaccac ccgccagcgg
    10261 cagtgagttg taacagctca caatcccgta gccccctttt gtgaaaattt tatcatgcac
    10321 cggaccgttg gcgatgtgcg gtttgctaca ttcacagatg ttcttcgcca cttccagcag
    10381 caggtcatca ggggtgattt caggatcgta gataaaggtc aggttcggtg aaacctgctt
    10441 caactctgcg tctgcacgta agatcgcgcg ggtaatgggc gaatcagacg ggccgatatt
    10501 ggcgtgcata aaggcgtctg gcagggttct gtcgaggtaa cgccagaaac gttttattcg
    10561 aacatcgatc tcgtcttgtg ttagaattct aacatatggt tgcaacaacg catccagttg
    10621 ccccaggtag accggcatcg atgtgaccga cggtacgtgg tggtaaagaa tggtcagcag
    10681 agagagtgcg tcatcaagat ctttcgcgcc ttccagctcc agccattcgg aaccgttcgc
    10741 cagaaaacgg gcgtaatcgg gtaagacata gcgcggtttg tacggcgcat gaccttcaaa
    10801 catatcgcag attacaccct catccagcgc gcggcgggct tcggcaggaa gctgcggata
    10861 aggcagattg ttttctgctt ccagtgccag aaaatggcgc ttctgctccg ggctaagcac
    10921 tgggctggtg acaatttgct ggcaacgttg ttgcagtgca ttttcatgag aagtgggcat
    10981 cttcttttcc ttttatgccg aaggtgatgc gccattgtaa gaagtttcgt gatgttcact
    11041 ttgatcctga tgcgtttgcc accactgacg cattcatttg aaagtgaatt atttgaacca
    11101 gatcgcatta cagtgatgca aacttgtaag tagatttcct taattgtgat gtatatcgaa
    11161 gtgtgttgcg gagtagatgt tagaatacta acaaactcgc aaggtgaatt ttattggcga
    11221 caagccagga gaatgaaatg actgatctga aagcaagcag cctgcgtgca ctgaaattga
    11281 tggacctgac caccctgaat gacgacgaca ccgacgagaa agtaattgct ctgtgtcatc
    11341 aggccaaaac cccggtcggc aataccgccg ctatctgtat ctatcctcgc tttatcccga
    11401 ttgctcgcaa aacgctgaaa gagcagggca ccccggaaat ccgaatcgct acggtaacca
    11461 acttcccaca cggtaacgat gacatcgaaa tcgcgctggc agaaacccgt gcggcaatcg
    11521 cctacggtgc tgatgaagtt gacgttgtgt tcccgtaccg cgcgctgatg gcgggtaacg
    11581 agcaggttgg ttttgacctg gtgaaagcct gtaaagaggc ttgcgcggca gcgaatgtac
    11641 tgctgaaagt gatcatcgaa accggcgaac tgaaagacga agcgctgatc cgtaaagcgt
    11701 ctgaaatctc catcaaagcg ggtgcggact tcatcaaaac ctctaccggt aaagtggctg
    11761 tgaacgcgac gccggaaagc gcgcgcatca tgatggaagt gatccgtgat atgggcgtag
    11821 aaaaaaccgt tggtttcaaa ccagcgggcg gcgtgcgtac tgcggaagat gcgcagaaat
    11881 atctcgccat cgcagatgaa ctgttcggtg ctgactgggc agatgcgcgt cactaccgct
    11941 ttggtgcttc cagcctgctg gcaagcctgt tgaaagcgct gggccacggt gacggtaaga
    12001 gcgccagcag ctactaagta agatgcttta cgcctgatgc gctgcgctta tcaggcctac
    12061 gagacgtatc tacccgtagg ccggataagg cgtagacgca tccggcaaaa gccgcctcat
    12121 actcttttcc tcgggaggtt accttgtttc tcgcacaaga aattattcgt aaaaaacgtg
    12181 atggtcatgc actgagcgat gaagaaattc gtttctttat caacggcatt cgcgacaaca
    12241 ctatctccga agggcagatt gccgccctcg cgatgaccat tttcttccac gatatgacaa
    12301 tgcctgagcg tgtctcgctg accatggcga tgcgagattc aggaaccgtt ctcgactgga
    12361 aaagcctgca tctgaatggc ccgattgttg ataaacattc cactggtggc gtcggcgatg
    12421 tgacttcgct gatgttgggg ccgatggtcg cagcctgcgg cggctatatt ccgatgatct
    12481 ctggtcgcgg cctcggtcat actggcggta cgctcgacaa actggaatcc atccctggct
    12541 tcgacatttt cccggatgac aaccgtttcc gcgaaattat taaagacgtc ggcgtggcga
    12601 ttatcggtca gaccagctca ctggctccgg cggataaacg tttctacgcg acccgtgata
    12661 ttaccgcaac cgtggactcc atcccgctga tcaccgcctc tattctggcg aagaaacttg
    12721 cggaaggtct ggacgcgctg gtgatggacg tgaaagtggg tagcggcgcg tttatgccga
    12781 cctacgaact ctctgaagcc cttgccgaag cgattgttgg cgtggctaac ggcgctggcg
    12841 tgcgcaccac cgcgctgctc accgacatga atcaggtact ggcctccagt gcaggtaacg
    12901 cggttgaagt tcgtgaagcg gtgcagttcc tgacgggtga gtatcgtaac ccgcgtctgt
    12961 ttgatgtcac gatggcgctg tgcgtggaga tgcttatctc cggcaaactg gcgaaagatg
    13021 acgccgaagc gcgcgcgaaa ttgcaagcag tgctggacaa cggtaaagcg gcagaagtct
    13081 ttggtcgtat ggtagcggca caaaaaggcc caaccgactt cgttgagaac tacgcgaagt
    13141 atctgccgac agcgatgctg acgaaagcag tctatgctga taccgaaggt tttgtcagtg
    13201 aaatggatac ccgcgcgctg gggatggcag tggttgcaat gggcggcggt cgtcgtcagg
    13261 catctgacac cattgattac agcgtcggct ttactgatat ggcgcgtttg ggcgaccagg
    13321 tagacggtca gcgtccgctg gcggttatcc acgcgaaaga cgaaaacagc tggcaggaag
    13381 cggcgaaagc ggtgaaagcg gcaattaaac ttgccgataa agcaccggaa agcacaccaa
    13441 ctgtctatcg ccgtatcagc gaataacggt atactgatct gatcatttaa atttgaagca
    13501 ctgagtacgg agaacatatg aaacgtgcat ttattatggt gctggactca ttcggcatcg
    13561 gcgctacaga agatgcagaa cgctttggtg acgtcggggc tgacaccctg ggtcatatcg
    13621 cagaagcttg tgccaaaggc gaagctgata acggtcgtaa aggtccgctc aatctgccaa
    13681 atctgacccg tctggggctg gcgaaagcac acgaaggttc taccggtttc attccggcgg
    13741 gaatggacgg caacgctgaa gttatcggcg cgtacgcatg ggcgcacgaa atgtcatccg
    13801 gtaaagatac cccgtctggt cactgggaaa tagccggtgt cccggttcta tttgagtggg
    13861 gatatttctc cgatcacgaa aacagcttcc cgcaagagct gctggataaa ctggttgaac
    13921 gcgctaatct gccgggttac ctcggtaact gccactcttc cggtacggtc attctggatc
    13981 agctgggcga agagcacatg aaaaccggca agccgatttt ctatacctcc gctgactctg
    14041 tgttccagat tgcctgccac gaagaaacct ttggcctgga taaactctac gaactgtgcg
    14101 aaatcgcccg tgaagagctg accaacggcg gctacaacat cggtcgtgtt atcgctcgtc
    14161 cgtttatcgg tgacaaagcc ggtaacttcc agcgtaccgg taaccgtcac gacctggctg
    14221 ttgagccgcc agcaccgacc gtgctgcaga aactggttga tgaaaaacac ggtcaggtgg
    14281 tttctgtcgg taaaattgca gacatctacg ccaactgcgg tatcaccaag aaagtgaaag
    14341 caactggcct ggacgcgctg tttgacgcca ccatcaaaga gatgaaagaa gcgggtgata
    14401 acaccatcgt cttcaccaac ttcgttgact tcgactcttc ctggggccac cgtcgcgacg
    14461 tcgccggtta tgctgtgggt ctggagctgt tcgaccgccg tctgccggag ctgatgtctc
    14521 tgctgcgcga tgacgacatc ctgatcctca ctgctgacca cggttgtgat ccgacctgga
    14581 ccggtactga ccacacgcgt gaacacattc cggtactggt ttacggcccg aaagtaaaac
    14641 cgggctcact gggtcaccgt gaaaccttcg cggatatcgg ccagactctg gcaaaatatt
    14701 ttggtacttc tgatatggaa tatggcaaag ccatgttctg ataatgggtg cggcttgatg
    14761 ccctcacccc ggccctctcc ctcagggaga gggtgaaaac atgttgataa ggataaaaca
    14821 atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt tttgatgcca
    14881 ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc ccgtgaagtg
    14941 aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa aatttccgta
    15001 atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact gatcaccgat
    15061 ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc gcacgtaaaa
    15121 ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa ccgcatccgt
    15181 tttaaagatc atgactttgc cgctatcgct gactttgaca tggtgcgtaa cgcggtagac
    15241 gcggctaaag cactgggcat tgatgctcgc gttggtaacc tgttctccgc tgacctgttc
    15301 tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcatcct cggcgtggaa
    15361 atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc cctgaccatc
    15421 tgcactgtgt ctgaccacat ccgcactcac gagcagacca ctgccgctga gcgtcagacc
    15481 accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga taaagagtaa
    15541 ttgtgtttcg ctgaaaggcg attgtcgtgt taagccggag tgggggactg ctccggcttt
    15601 ttagtatcta ttcatcaa
//
LOCUS       DAZSBF010000089        15582 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZSBF010000089 DAZSBF010000000
VERSION     DAZSBF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.090"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..2065
                     /locus_tag="ACHXWD_002206"
     CDS             <1..2065
                     /locus_tag="ACHXWD_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR7621117.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2081..3115
                     /locus_tag="ACHXWD_002207"
     CDS             2081..3115
                     /locus_tag="ACHXWD_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR7621118.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHXWD_002208"
     CDS             complement(3197..3535)
                     /gene="rcnB"
                     /locus_tag="ACHXWD_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR7621119.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHXWD_002209"
     CDS             complement(3754..4578)
                     /gene="rcnA"
                     /locus_tag="ACHXWD_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR7621120.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHXWD_002210"
                     /pseudo
     CDS             4699..4971
                     /gene="rcnR"
                     /locus_tag="ACHXWD_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5063..5159
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5194..5982
                     /gene="thiM"
                     /locus_tag="ACHXWD_002211"
     CDS             5194..5982
                     /gene="thiM"
                     /locus_tag="ACHXWD_002211"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR7621121.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5979..6779
                     /gene="thiD"
                     /locus_tag="ACHXWD_002212"
     CDS             5979..6779
                     /gene="thiD"
                     /locus_tag="ACHXWD_002212"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR7621122.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6844..7662
                     /locus_tag="ACHXWD_002213"
     CDS             6844..7662
                     /locus_tag="ACHXWD_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR7621123.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7714..8460
                     /locus_tag="ACHXWD_002214"
     CDS             7714..8460
                     /locus_tag="ACHXWD_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR7621124.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8434..9399)
                     /locus_tag="ACHXWD_002215"
     CDS             complement(8434..9399)
                     /locus_tag="ACHXWD_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR7621125.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9396..10400)
                     /locus_tag="ACHXWD_002216"
     CDS             complement(9396..10400)
                     /locus_tag="ACHXWD_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR7621126.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10397..11674)
                     /locus_tag="ACHXWD_002217"
     CDS             complement(10397..11674)
                     /locus_tag="ACHXWD_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR7621127.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHXWD_002218"
     CDS             11931..12983
                     /gene="fbaB"
                     /locus_tag="ACHXWD_002218"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR7621128.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13302..14156
                     /gene="gatY"
                     /locus_tag="ACHXWD_002219"
     CDS             13302..14156
                     /gene="gatY"
                     /locus_tag="ACHXWD_002219"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR7621129.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHXWD_002220"
                     /pseudo
     CDS             14185..15442
                     /gene="gatZ"
                     /locus_tag="ACHXWD_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15452..>15582
                     /locus_tag="ACHXWD_002221"
     CDS             15452..>15582
                     /locus_tag="ACHXWD_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR7621130.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4088 a         3728 c         3987 g         3779 t
ORIGIN      
        1 tggctatgtt ccaccggaaa actgggagcg gggtattaat gcgttttata cctcttatta
       61 tgtgagtcag tattacagtg actataaagc gtcgggtaat agtaagagta catatgtacg
      121 ttttaacagc gggttaaatt tactggggtg gcaactgcat tctgatgcca gtttcagtaa
      181 aacaaataac aatccagggg tgtggaaaag caataccctg tatctggaac gtggatttgc
      241 ccaacttctc ggcacgcttc gcgtgggtga tatgtacaca tcaagcgata tttttgattc
      301 tgttcgcttc agaggtgtgc ggttgtttcg tgatatgcag atgttgccta actcgaaaca
      361 aaattttacg ccacgggtgc aggggattgc tcagagtaac gcgctggtaa ctattgaaca
      421 gaatggtttt gtggtttatc agaaagaggt tcctcctggc ccgtttgcga ttacagattt
      481 gcagttggcc ggtggtggag cagatcttga tgtcagcgtg aaagaggcgg acggttcggt
      541 aaccacctat ctggtgcctt atgcagcggt gccaaatatg ctgcaacccg gcgtgtcgaa
      601 atatgatttt gcggcgggtc gtagccatat tgaaggggcg agcaaacata gtgattttgt
      661 ccaggcgggt tatcagtatg gttttaataa cttattgacg ttgtatggtg gctcgatggt
      721 tgcgaataat tattacgcgt tcactttggg gactggctgg aatacacgca ttggtgccat
      781 ttccgtcgat gccacgaagt cgcatagtaa acaagacaac ggcgatgtgt ttgacgggca
      841 aagttatcaa attgcctaca acaaatttgt gagccaaacg tcgacgcgtt ttggtctggc
      901 ggcctggcgt tattcgtcgc gtgattaccg gacatttaac gatcacgttt gggcaaacaa
      961 taaagataat tatcgccgtg atgaaaacga tgtctatgac attgccgatt attaccagaa
     1021 tgattttggt cgcaaaaata gcttttccgc caatatgagc cagtcattgc cagaaggttg
     1081 ggggtctgtg tcattaagta cgttatggcg agattactgg gggcgtagcg gcagtagtaa
     1141 ggattatcag ttgagttatt ccaacaacct gcgacggata agctataccc tcgcggcaag
     1201 ccaggcttat gacgagaatc atcatgaaga gaaacgtttt aatattttta tatcgattcc
     1261 ctttgattgg ggtgatgacg tttcgacgcc tcgtcggcaa atatatatgt ctaactcaac
     1321 gacgtttgat gatcaggggt ttgcctcaaa taatacggga ttatcaggaa cagtagggag
     1381 tcgggatcag ttcaattatg gtgtcaacct gagtcatcaa catcagggaa atgaaacgac
     1441 agctggagcg aatttaacct ggaacgcgcc ggtggcaaca gtgaatggca gttatagtca
     1501 gtcgagtact tatcgacagg ctggagccag tgtttcaggg ggcattgtcg cctggtcggg
     1561 tggcgttaat ctggcgaacc gtctttccga aacgtttgct gtgatgaatg cgccaggaat
     1621 taaagatgct tatgtcaatg ggcaaaaata tcgcacaaca aaccgtaatg gagtggtggt
     1681 atacgacgga atgacacctt atcgggaaaa tcacctgatg ctggatgtgt cgcaaagcga
     1741 tagcgaagca gaattacgtg gcaaccggaa aattgccgca ccttatcgcg gcgcggttgt
     1801 actggttaat tttgataccg atcagcgcaa gccctggttt ataaaagcgt taagagcgga
     1861 tgggcaacca ttaacgtttg gttatgaagt caatgatatc cacggtcata atattggcgt
     1921 tgtcggccag ggaagccagt tatttattcg caccaatgaa ataccgccat cggttaatgt
     1981 agcaattgat aagcaacaag gactttcatg cacaatcacc ttcggtaaag agattgatga
     2041 aagtagaaat tatatttgcc agtaatagtc aggtggttct atggaaattc gcataatgct
     2101 atttatatta atgatgatgg ttatgcctgt gagctatgcg gcatgttata gtgagttatc
     2161 tgttcagcac aatttggttg ttcaggggga ttttgcactt actcaaacac aaatagcgac
     2221 atatgagcat aattttaatg attcgtcatg cgtgagtaca aatactatca cccctatgag
     2281 ttcgtcggat attattgttg ggctttataa cgataccata aaattaaatt tacattttga
     2341 atggaccaat aaaaacaaca tcacgttgtc aaataatcag accagtttca ccagtggtta
     2401 ttcagttacg gtgacacctg cggccagtaa tgcaaaagtg aatgtttctg cggggggcgg
     2461 cggttcagtg atgattaatg gtgttgcgac attatccagt gcttcatcat cgacacgcgg
     2521 gagtgccgca gtacaatttc tactgtgttt attaggtggc aagtcatggg atgcatgtgt
     2581 aaatagctac agaaatgcat tggcacaaaa tgcaggtgtc tattccttta atctgacatt
     2641 gtcatacaac ccgataacca caacctgcaa accggacgat ttattaatta ctttagacag
     2701 tattcccgtt tcacaattac cagccacagg taacaaagca acaataaata gtaaacaagg
     2761 ggatattatt ctgcgttgta aaaatttatt aggtcaacaa aatcaaacat cacggaaaat
     2821 gcaggtgtat ttatcaagtt ctgatttgtt agccggcagt aacacgatcc tgaaaggtgc
     2881 agaaaataat ggagtcggat ttatgcttga aagcaatggt tctccagtca catttttaaa
     2941 tatcactgat aacagcaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact
     3001 tacagataca acggtttcaa ttccgataac tgccagttac tacgtctacg atacaaacaa
     3061 agttaaatct ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca
     3121 agcaagacga gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat
     3181 gatgaggggg ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt
     3241 taccgtcggt gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg
     3301 catcaggcgc gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt
     3361 acatctctgg gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta
     3421 actcatactt agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg
     3481 cggcactggt aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact
     3541 ctccactttg gggatctgta atatcagtcg catatctttc ctgccataca atataactga
     3601 caagcgatat tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc
     3661 taaaagcatc cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg
     3721 gctgaagata acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg
     3781 tacgcccata tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt
     3841 agcgagagtg ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc
     3901 aacgcctacg gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc
     3961 gcccagtgtc agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca
     4021 ggggataagg ccaccggtta agccaaatag caaaatttgc cagttggtga cctctctacc
     4081 atcaaagcgt cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc
     4141 atgttcgtga tggtgatgat gtccatggtc gtggtgatct tcgtgatcgt gatgatgatg
     4201 ctcataatca tactcgtgca tattctccag ccagttgcgt tcgccgcgcc aggtacgcca
     4261 gaacatccag aacgcggtgc caataatgat cactgcggaa atcagctgga gccacggttc
     4321 tgctgattga gcagtaaagc gcttgctgat caccatcccg ccaaaggcaa ttaaccagac
     4381 cactgcggta tgcgaaatag ttgctgccag tccgagcatc accgcttgtt taatggtgcc
     4441 tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt ccaggccatg
     4501 aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt gctgaagaag
     4561 agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct gattctactc
     4621 cccccagtac ctgatactac cccccagtag attaatagtg ctatgatatt ttcatattct
     4681 tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact gaaagcgcgt
     4741 gccagtaaga ttcagggcta ggtcgtggcg ctcaagaaaa tgctcgacga gccgcatgaa
     4801 tgcgctgcag ttttacaaca gattactgct atccgtggcg cggtaaacgg tctgatgcgg
     4861 gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga gctaaaacgt
     4921 gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata atttattaac
     4981 gcgattgtaa aactgccgtt tttcctcgtt tacaacgcgt gcgctggaca ttaccatcct
     5041 cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt gaaggctgag
     5101 aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg accaccaggt
     5161 cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct gggttcagcg
     5221 caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca ctgcatgacc
     5281 aatgatgtgg tgcaaacctt taccgctaat accttgctgg cgctcggtgc atcgccagcg
     5341 atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc cttgttgatt
     5401 aacgttggca cactgacgca gccacgcgct caggcgatgc gtgctgccgt tgagcaagca
     5461 aaaagctctc aaacaccctg gacgcttgat ccagtagcgg ttggtgcgct cgattatcgc
     5521 cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg taatgcttcg
     5581 gaaatcatgg cattagctgg cattgctaat ggtggacggg gagtggatac cactgacgcc
     5641 gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc aatcgtcgtg
     5701 gtcactggcg aggtggatta tgttaccgat ggccatcgcg ccgttggcat tcacggtggc
     5761 gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt tgtcgctgcc
     5821 tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca ctggatgaaa
     5881 caagccggag agcgcgcagt cgccagaagc gaggggctag gcagttttgt tccacatttc
     5941 cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt aacgctctga
     6001 cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt aaaaccttct
     6061 cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa aatacccgtg
     6121 gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc gattcggtgt
     6181 tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc gatattgttg
     6241 aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc gacaccgtta
     6301 tgctggcaaa aagcggcgac ccactgcttt caccttcggc agttgccacg ctacgcagtc
     6361 gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagcagcc gccttgctcg
     6421 acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg ctgttggcga
     6481 tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa agcccggact
     6541 ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg accaaaaaca
     6601 cccacggtac tggttgtaca ctctctgcgg cgttggctgc actacgcccg cgccatacaa
     6661 actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta gcccaggccg
     6721 acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc tggtggtgag
     6781 caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc agggatgaaa
     6841 ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg cgctcttggg
     6901 ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta ttcgacagca
     6961 gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc tttcgctatt
     7021 cgtattcctg ccagttacac cattcacgga atagatgttt cgcgctggca ggagcggatc
     7081 gactggcagc gtgtggcaaa aatgcgcgat aacggtattc gcttacaatt tgcctttatt
     7141 aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg gcaactaagc
     7201 cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt agcagcaccg
     7261 gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt ccctgcggta
     7321 ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag ggtaagtcag
     7381 tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc aggagccgtt
     7441 ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt ggctcactac
     7501 tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca ttccgaccgt
     7561 ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg cacggtggag
     7621 gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag cagtgatata
     7681 ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac ccagctgatt
     7741 gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat taagtttgcc
     7801 gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat tttgcccggt
     7861 gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa ggcgatgcag
     7921 gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca gatcaacaac
     7981 atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt gttgcgcggg
     8041 aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga agaagtcgcg
     8101 cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat tcgctatgtc
     8161 gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat tcatgatgtt
     8221 gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta cccacaacgt
     8281 acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca tattcagtta
     8341 gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca gcaacggcct
     8401 attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg cgaggagtag
     8461 ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg acgccactgc
     8521 attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg caagtacgcc
     8581 acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa atgccggaat
     8641 gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac gaacgatcaa
     8701 cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg cggataaagc
     8761 aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag gtcgacaggc
     8821 catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa aatcgataaa
     8881 cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc agggcgaggc
     8941 cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc gcgccagcca
     9001 ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt cgccatccgg
     9061 ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca ggctgattaa
     9121 gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac cgagcggcaa
     9181 ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca gtgcgcagcc
     9241 gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg ggagggcata
     9301 agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct gacgcgtggt
     9361 taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg ttgttgacga
     9421 tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc atccagttcc
     9481 gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat tgccgtcgcc
     9541 atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc gcagcgattc
     9601 gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc aatagtgctg
     9661 gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc gtcggcattg
     9721 cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa cgtagtgatg
     9781 cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc tgaccagctt
     9841 tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc aaccgccaga
     9901 tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga atcaacatca
     9961 cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt ggtcacgccg
    10021 ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc aatcttcgag
    10081 gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc gagaatatta
    10141 cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc cgccagacac
    10201 agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc cgcgttattc
    10261 tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac gcggctgcgt
    10321 ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata aagagcaccg
    10381 agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg cgatcatcga
    10441 ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac agcacggcga
    10501 taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa gtcagtccgt
    10561 ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga cgatagccga
    10621 gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg gtacgcatat
    10681 gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa tcgtaactta
    10741 cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca tccgtactac
    10801 cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat aatacctttt
    10861 tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt tcagagaact
    10921 ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga taaccgttgg
    10981 caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag aaaaagacga
    11041 ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact ttaatatcca
    11101 ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca ccgagcagag
    11161 cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg gcatacccca
    11221 gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata gtgcccatca
    11281 cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg atgctgttag
    11341 tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga aagaacccgg
    11401 caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac atcaataccg
    11461 ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga attggcgaca
    11521 ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca ctaaaaccgc
    11581 ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac cattcaacaa
    11641 acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat gacaggtaat
    11701 gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt gcggtgaaaa
    11761 cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct gaaaaaacat
    11821 ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc aaaaggcggg
    11881 ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta atgacagata
    11941 ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt atgacaattc
    12001 cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg attgacaata
    12061 atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg cgtctggctg
    12121 gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct gccggagctt
    12181 catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg gcgatcgaag
    12241 cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg cggcgctatg
    12301 cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt tacccgaata
    12361 cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc gcggtggcgg
    12421 ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa gaaatttctg
    12481 cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc tatttgcgaa
    12541 actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg accggtcagg
    12601 caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg gcggaaaata
    12661 acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat agcaaattga
    12721 ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat atgggccggg
    12781 ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt gatgcagtgc
    12841 gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga cgtaaagcgt
    12901 tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac gtttatctcg
    12961 atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc gtaggacgga
    13021 taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag cgtcttatca
    13081 tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc cggcataaaa
    13141 acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct ttcactcctc
    13201 gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa caaatcattt
    13261 cattatattt tgaaatcgaa aacaagcaac aggatatgaa aatgtacgtg gtatcgacaa
    13321 agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc aatattcaca
    13381 atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg ccggtcatca
    13441 tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg gcgctggtca
    13501 gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat cacacgaaat
    13561 ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt gacgcctcgc
    13621 atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt tgccatcgct
    13681 ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat gatgtgcaag
    13741 tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt gccgaggcaa
    13801 ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat gccagcgcac
    13861 cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta ccgctggtgc
    13921 tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa ctggggatat
    13981 gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta aaaaattacc
    14041 tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct aaatccgcaa
    14101 tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg gcataacgca
    14161 cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa agctggtgaa
    14221 catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc ggcgctggca
    14281 tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca ggtcaatcaa
    14341 tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt tacgattgcc
    14401 gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct ggggccaaac
    14461 tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct ggtaaaagca
    14521 tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg cgcgggggat
    14581 cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg ctgcggaaag
    14641 tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg aagttccggt
    14701 tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg aagatgccgc
    14761 caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag aggcgttaac
    14821 acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca atattatcca
    14881 ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc gaatggttta
    14941 tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag tccgcgatca
    15001 ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg cgatatttgc
    15061 actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt gcctggcggt
    15121 aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc gtacgggttt
    15181 taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt attactggcc
    15241 gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag gcgtggacat
    15301 cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc agtccgggga
    15361 attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt tgcgcgccta
    15421 tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc gtagcggaat
    15481 ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc tcgccaaagg
    15541 tgtggttcat gatacctggc cacaggcatt aattgccaga ga
//
LOCUS       DAZSBF010000090        15337 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZSBF010000090 DAZSBF010000000
VERSION     DAZSBF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.091"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            99..851
                     /locus_tag="ACHXWD_002222"
                     /pseudo
     CDS             99..851
                     /locus_tag="ACHXWD_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
     gene            complement(892..1824)
                     /locus_tag="ACHXWD_002223"
     CDS             complement(892..1824)
                     /locus_tag="ACHXWD_002223"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR7621131.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2188..4974
                     /gene="polA"
                     /locus_tag="ACHXWD_002224"
     CDS             2188..4974
                     /gene="polA"
                     /locus_tag="ACHXWD_002224"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR7621132.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHXWD_002225"
     CDS             complement(5356..5988)
                     /gene="yihA"
                     /locus_tag="ACHXWD_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR7621133.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6285..6451
                     /locus_tag="ACHXWD_002226"
                     /pseudo
     CDS             6285..6451
                     /locus_tag="ACHXWD_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6570..7079
                     /gene="yihI"
                     /locus_tag="ACHXWD_002227"
     CDS             6570..7079
                     /gene="yihI"
                     /locus_tag="ACHXWD_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR7621134.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7268..8641
                     /gene="hemN"
                     /locus_tag="ACHXWD_002228"
     CDS             7268..8641
                     /gene="hemN"
                     /locus_tag="ACHXWD_002228"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR7621135.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8831..8941)
                     /locus_tag="ACHXWD_002229"
     CDS             complement(8831..8941)
                     /locus_tag="ACHXWD_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR7621136.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHXWD_002230"
     CDS             complement(9053..10462)
                     /gene="glnG"
                     /locus_tag="ACHXWD_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR7621137.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHXWD_002231"
     CDS             complement(10474..11523)
                     /gene="glnL"
                     /locus_tag="ACHXWD_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR7621138.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHXWD_002232"
     CDS             complement(11697..13106)
                     /gene="glnA"
                     /locus_tag="ACHXWD_002232"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR7621139.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13479..15302
                     /gene="typA"
                     /locus_tag="ACHXWD_002233"
     CDS             13479..15302
                     /gene="typA"
                     /locus_tag="ACHXWD_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR7621140.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4004 a         3829 c         3937 g         3567 t
ORIGIN      
        1 cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca cctcaaaaac
       61 accatcatac actaaatcag taagttggca gcatcaccag cgagtcgaaa tttaatcaga
      121 ttaacattat caatcacggg gaagacgttg cccaaatcga tgctttcgtt gcaaaaacca
      181 tgctggatcg cgttaaagac aaagattata tcaatgtcaa tctgacctac gaacttgata
      241 agttaacaaa agggaatcag caactcggta gtggtgagtg gtcattgatt gctgaatcta
      301 ttgatccctc agcagtgcgc caatttatca tccagtataa cattgctatg cagaagcagc
      361 ttgctgtaca ccctgagtta gcaaacgatg aagttgctct gcaagaagtg aatgctgcat
      421 tgttcaaaga gtatttaccg ttattacaaa aaagtgagcc gaccattaaa caaccggtaa
      481 gatggaagaa cgcactcggc gaactaaatg ccaatctgga tatcagtatt gccgacccag
      541 ccaaatcttc atcatccaca aacaaagata tcaaattgct caattttgat gtgaagttac
      601 cgcttaatgt cgtcacagaa accgcaaaac agcttaattt atctgaagga atggatgcgg
      661 aaaaagcgca aaagcaggct gataaacaaa tcagcgggat gatgacattg ggtcagatgt
      721 ttcagttaat cacgattgac aacaataccg cctcgctgca gctgcgttat acaccgggta
      781 aagttgtgtt taacggacag gagatgagcg aagaagaatt tatgtctcgt gccggacgtt
      841 ttgttcatta aaatactcac tcgcctctgc tcattcagag gcgagtaaaa cttacttatt
      901 ctgacgctgt gatgacatga gagacgtcag aagccggtct ttttcctgcc acaatgagtt
      961 cagccattgc tgaaaatggc gcttaaagct tttgtcattg atgtagtcgc catgtaactc
     1021 atcggcaata ggctgtaaat ccacatgtac gacaatccgc gtcagtttac cgcttaacat
     1081 atcgaagaac ggctgacggt tattgtccgg ataacacagc gtaacattaa gcagtttatc
     1141 gaattgttta cccagtacat ttagcgccat cgcaatgcct gccgcctttg gtggcaacaa
     1201 gttttgaaaa gtggagtggg tttgctgatg tttttcttgc gtgaaacggg agccttcaac
     1261 gaaattgaca atggtggtgg gatgcaggcg aaacttttca caagaacgcc gagtggtttc
     1321 aacatcttta ccacgtcgct ccggatgacg taataaataa gcgcgggaat aacgcttcat
     1381 aaacggcata tccagcgccc agcacgccag gccaagaaat ggcacccagg ccagctgttg
     1441 cttgaggaaa tatttattca tcggaatgtg cttacgaaac agcacgcaca gtacgacaat
     1501 atctgcccaa ctacggtggt tacaaataag cagataccag ttcttcttac ttaacccttc
     1561 cagcccgtga acttcccatt gcaggtgtgg gttcagatgc agtaataccg ccagaccttc
     1621 acaccagcaa tacatcataa aatcacaaaa acgcgaaacc tttcgccaaa tgactggcac
     1681 aggtagcaat agttttacaa tcccggcaat gatgatcggg acggaacaaa aaatagtgac
     1741 caaaatggtc aatacgatac tcagcaaaag ggttatcgca gcgagtattc tcgtcataat
     1801 gaatttattc aaaaggttag ccatagacag tgcgctaaag aagcgcaagg cgcagatttt
     1861 agcagaaaac gacccaaata acggatgatc cttaaggaga aaaataatcc atatctatcc
     1921 acattagaaa aaatcccatt atctcaatta ttagggatgg ctttattttt aactgcatga
     1981 aaaacaaaga caaacatcat gctgtaaaaa gcatgataat aaattaaaag cgatgtaaat
     2041 aatttatgca caaagttatc cacatgacga tttgcgagcg atccagaaga tctacaaaag
     2101 attttcacga aaagcggtga aaaactcatg ttttcatcct gtctgtggca tcctttaccc
     2161 ataatctgat aaacaggcac ggacattatg gttcagatcc cccaaaatcc acttatcctt
     2221 gtagatggtt catcttatct ttatcgcgca tatcacgcgt ttcccccgct gactaacagc
     2281 gcaggcgagc cgaccggtgc gatgtatggt gtcctcaaca tgctgcgcag tctgatcatg
     2341 caatataaac cgacgcatgc agcggtggtc tttgacgcca agggaaaaac ctttcgtgat
     2401 gaactgtttg aacattacaa atcacatcgc ccgccaatgc cggacgatct gcgagcacaa
     2461 atcgaacccc tgcacgcgat ggttaaagcg atgggactgc cgctgctggc ggtttctggc
     2521 gtagaagcgg acgacgttat cggtactctg gcgcgcgaag ccgaaaaagc cgggcgtccg
     2581 gtgctgatca gcactggcga taaagatatg gcgcagctgg tgacgccaaa tattacgctt
     2641 atcaacacca tgacgaatac catcctcgga ccggaagagg tggtgaataa gtacggcgtg
     2701 ccgccagaac tgatcatcga tttcctggcg ctgatgggtg actcctccga taacattcct
     2761 ggcgtaccgg gcgtcggtga aaaaaccgcg caggcattgc tgcaaggtct tggcgggctg
     2821 gatacgctgt atgccgagcc agaaaaaatt gctgggttga gcttccgtgg cgcgaaaaca
     2881 atggcagcga agctcgagca aaacaaagaa gttgcttatc tctcatacca gctggcgacg
     2941 attaaaaccg acgttgaact ggagctgacc tgtgaacaac tggaagtgca gcaaccggca
     3001 gcggaagagt tgttggggct gttcaaaaag tatgagttca aacgctggac tgctgatgtc
     3061 gaagcgggca aatggttaca ggccaaaggg gcaaaaccag ccgcgaagcc gcaggaaacc
     3121 agtgttgcag acgaagcgcc agaagtgacg gcaacggtga tttcttatga caactacgtc
     3181 accatccttg atgaagaaac actgaaagcg tggattgcga agctggaaaa agcgccggta
     3241 tttgcatttg ataccgaaac cgacagcctt gataacatct ctgctaacct ggtcgggctt
     3301 tcttttgcta tcgagccagg cgtagcggca tatattccgg ttgctcatga ttatcttgat
     3361 gcgcccgatc aaatctctcg cgagcgtgca ctcgagttgc taaaaccgct gctggaagat
     3421 gaaaaggcgc tgaaggtcgg gcaaaacctg aaatacgatc gcggtattct ggcgaattac
     3481 ggcattgagc tgcgtgggat tgcgtttgat accatgctgg agtcctacat tctcaatagc
     3541 gttgccgggc gtcacgatat ggacagcctc gcggaacgtt ggttgaagca caaaactatc
     3601 acttttgaag agattgcggg taaaggcaaa aatcaactga cctttaacca gattgccctc
     3661 gaagaggccg gacgttacgc cgccgaagat gcggatgtca ccttgcagtt gcatctgaaa
     3721 atgtggccag atctgcaaaa acacaaaggg ccgttgaacg tcttcgagaa tatcgaaatg
     3781 ccgctggtgc cggtgctttc acgcattgaa cgtaacggtg tgaagatcga tccgaaagtg
     3841 ctgcacaatc attctgaaga gctcaccctt cgtctggctg agctggaaaa gaaagcgcat
     3901 gaaattgcag gtgaggaatt taacctttct tccaccaagc agttacaaac cattctgttt
     3961 gaaaaacagg gcattaaacc gctgaagaaa acgccgggtg gcgcgccgtc aacgtcggaa
     4021 gaggtactgg aagaactggc gctggactat ccgttgccaa aagtgattct ggagtatcgt
     4081 ggtctggcga agctgaaatc gacctacacc gacaagctgc cgctgatgat caacccgaaa
     4141 accgggcgtg tgcatacctc ttatcaccag gcagtaactg caacgggacg tttatcgtca
     4201 accgatccta acctgcaaaa cattccggtg cgtaacgaag aaggtcgtcg tatccgccag
     4261 gcgtttattg cgccagagga ttatgtgatt gtctcagcgg actactcgca gattgaactg
     4321 cgcattatgg cgcatctttc gcgtgacaaa ggcttgctga ccgcattcgc ggaaggaaaa
     4381 gatatccacc gggcaacggc ggcagaagtg tttggtttgc cactggaaac cgtcaccagc
     4441 gagcaacgcc gtagcgcgaa agcgatcaac tttggtctga tttatggcat gagtgctttc
     4501 ggtctggcgc ggcaattgaa cattccacgt aaagaagcgc agaagtacat ggacctttac
     4561 ttcgaacgct accctggcgt gctggagtat atggaacgca cccgtgctca ggcgaaagag
     4621 cagggctacg ttgaaacgct ggacggacgc cgtctgtatc tgccggatat caaatccagc
     4681 aatggtgctc gtcgtgcagc ggctgaacgt gcagccatta acgcgccaat gcagggaacc
     4741 gccgccgaca ttatcaaacg ggcgatgatt gccgttgatg cgtggttaca ggctgagcaa
     4801 ccgcgtgtac gtatgatcat gcaggtacac gatgaactgg tatttgaagt tcataaagat
     4861 gatgttgatg ccgtcgcgaa gcagattcat caactgatgg aaaactgtac ccgtctggat
     4921 gtgccgttgc tggtggaagt ggggagtggc gaaaactggg atcaggcgca ctaagattcg
     4981 cctgaacatg cccttttttc gtaagtaagc aacataagct gtcacgtttt gtgatggcta
     5041 ttagaaattc ctatgcaaca actgaaaaaa aattacaaaa agtgctttct gaactgaaca
     5101 aaaaagagta aagttagtcg cgtagggtac agaggtaaga tgttctatct ttcagacctt
     5161 ttacttcacg taatcggatt tggctgaata ttttagccgc cccagtcagt aatgactggg
     5221 gcgtttttta ttgggcgaaa gaaaagatcc gtaatgcctg atgcgctatg tttatcaggc
     5281 caacggtaga attgtaatct attgaattta cgggccggat acgccacatc cggcacaagc
     5341 attaaggcaa gaaaattatt cgccgtcctg cgtttcttct acaggctgca tctcgctaaa
     5401 ccaggtatcc agtttctgcc gcagcttgtc cacgccttgt ttcttcaacg aagaaaacgt
     5461 ttcaacctgc acatcaccgt taaacgccag tacagcttca cgcaccatat tcaattgcgc
     5521 tttacgtgcg ccgcttgcca gtttgtccgc tttggtcagc agcaccagaa cggcgatatt
     5581 gctgtctacc gcccactcaa tcatctgctg atccaaatct ttcagcggat ggcgaatatc
     5641 cattagcacc accagacctt gcaggctctg acgtttttcg aggtattcgc cgagcgcacg
     5701 ctgccatttg cgcttcatct cttccgggac ttccgcataa ccgtacccag gcaagtcaac
     5761 cagacgcttg ccgtcagcca cttcaaacag gttgataagc tgggtgcgcc ctggggtttt
     5821 tgaggtacga gccaggcttt tctggttagt cagcgtgttc agcgcgctgg atttacctgc
     5881 gttggaacgg cctgcaaaag ccacttcaat tccggtatcg gaaggtaggt ggcgaatatc
     5941 aggcgcactc atcacaaaat gcgtctgttg ataattcaaa ttagtcaaag cggtcgtctc
     6001 cgtcagtcaa agctttgtgg cgattatacc tgaaccataa taaaaggcgg atttttcggc
     6061 gtgagcgttg taagtaaaag ccatacgctt tgtgagacat tgccgatagt ctttatgcga
     6121 aatagcagag aaaattctgc gatgcatgtc aaataagcta tataaatcag tgaattgctt
     6181 ttatataaaa ctctgaaaat gtgcaaatac tgatggcggt tgattgtttg tttaaagcaa
     6241 aggcgtaaag tagcacccat agagcgagga cgctaacagg aacaatgact caggatgagg
     6301 gtcaggagcg ccaggaggcg aagacagagg attgtcagga agacaaacgt ccggagacgt
     6361 aattaaacgg aaatggaatc aacacggatt gttccggcta aaggaaaaac agggtgtgtt
     6421 ggcggcctgc aaggattgta agacccgtta agggttatga gtcaggaaaa aaggcgacag
     6481 agtaatctgt cgcctttttt ctttgcttgc tttctgttag attccgccgc aaatctatac
     6541 tgaataaaac ggctaaaaga cgaaccatta tgaaaccatc atcttcaaac tcacgcagca
     6601 aaggtcatgc aaaagcgcgt cgaaaaacac gcgaggagct ggatcaggaa gctcgtgatc
     6661 gcaagcgtca gaaaaaacgt cgtggtcatg cgccgggcag ccgcgcagcg ggcggaaaca
     6721 ccacgtcagg cagcaaaggc cagaacgcac caaaagatcc acgtattggc agtaaaaccc
     6781 ctattccatt gggcgtggct gaaaaagtca ccaaacagca caaaccgaag agtgagaaac
     6841 ctatgctttc accgcaggcg gagttggaat tactggaaac ggatgagcgt ctggatgcgc
     6901 tgctggaacg tctggaagca ggcgaaaccc tgagcgccga agagcaatcc tgggtggatg
     6961 ctaaactgga tcgtattgat gagttgatgc agaaactcgg cctctcttat gacgatgacg
     7021 aagaagagga agaagacgag aagcaagaag acatgatgcg tctgctgcgg ggtaactaac
     7081 ggattgccac cgtgggcctt cccgttctgc tgataaccct tccggttata tgttatctgg
     7141 tgtggttatt cgttaaacta cagcggttgt cgcggcgaca aaagtggctg cgcaaccggc
     7201 tgatgacccg aaacgggcat ccgccggtac gccgtagccg ccagagacgc catcggaagg
     7261 agtgagcatg tctgtacagc aaatcgactg ggatctggcc ctgatccaga aatataacta
     7321 ttccgggcca cgatacacct cgtacccgac cgcgctggag ttttcagaag acttcggcga
     7381 acaggcgttt ttacaagccg tggcgcgcta tcctgagcgt ccattatctc tctacgtaca
     7441 tatcccgttc tgccataagc tttgttactt ctgcggttgc aataagattg ttactcgcca
     7501 gcagcacaag gccgatcagt atctggacgc gctggagcaa gaaatcgtcc atcgtgcacc
     7561 gctgttcgcc gggcgtcacg tcagccaatt gcactggggc ggcggaacgc cgacgtatct
     7621 gaataaagcg caaatcagcc gcctgatgaa gctgctgcgc gaaaacttcc agttcaatgc
     7681 cgatgcggag atttcgatcg aagtcgatcc gcgggaaatc gaactggatg tactcgatca
     7741 tttacgcgcc gaaggcttta atcgcctgag catgggcgtg caggacttca acaaagaagt
     7801 gcaacgtctg gttaaccgcg agcaggatga agagttcatc tttgcactgc ttaaccatgc
     7861 gcgtgagatt ggttttacct ccaccaacat cgacctgatt tacggcctgc cgaaacagac
     7921 gccggagagt ttcgccttta ccctgaaacg tgtggcggag ctgaaccccg atcgtctgag
     7981 tgtctttaac tacgcgcatc tgccgaccat ttttgctgct cagcgcaaaa tcaaagatgc
     8041 tgacctgccg agtccgcagc aaaaactcga tatcctgcag gaaaccatcg ccttcctgac
     8101 gcaatcgggc tatcagttta tcggtatgga tcactttgcc cgtccggatg acgagctggc
     8161 ggtggcccag cgtgaaggcg tgctgcatcg taacttccag ggctacacca ctcagggcga
     8221 taccgatctg ctggggatgg gcgtttccgc catcagcatg attggcgact gctacgcgca
     8281 gaaccagaaa gagttgaagc agtactatca gcaagtggat gaacaaggca atgctctgtg
     8341 gcgtggtatt gcgctaacgc gtgatgactg tattcgccgc gatgtgatta agtcgctcat
     8401 ctgcaacttc cgtctggatt acgcccctat tgagaaacag tgggatttgc acttcgctga
     8461 ttactttgcg gaagatctca agctgctcgc cccgttagca aaagatgggc tggtggatgt
     8521 ggatgagaag ggaatacagg tgacggcgaa aggtcgcttg ctgatccgca acatttgcat
     8581 gtgctttgat acctatctgc gccagaaagc gcggatgcag cagttctcac gggtgattta
     8641 aatagtgaat ggcgcttcgt ttacaaagta gaaaacgaaa gcgccatcaa tgtgctgaga
     8701 acaagattgc ctgatgcgct acgcttatca ggcctacaaa acctgttgaa aatattgctt
     8761 ttgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagagca cttcatcagc
     8821 aatctgaaac tcaactaaac agcccaatca ttgcggcaca cagcacggca gcaacggcag
     8881 tttgcggtgt gtggctgtca actgcgccac tcgataccag attaattatt gattccagca
     8941 taatgactct ccccgttttc cgggcaagat catactgaac ttatcggaac agtaaagcgt
     9001 aaaataccag caatttgcgc tcaataatca atctttacac acaagctgtg aatcactcca
     9061 tccccagctc ttttaactta cgcgtcaggg tgttgcggcc ccagccgagt agccgcgccg
     9121 cttcctgttt atgcccctgc gtatgtcgta acgcggtcgt cagtaacgtc cgctccagct
     9181 ctggctgcgc ttcggaaagc agattttgat gaccggaacg cagcgctctg tctgcccact
     9241 gcgcaagaag cgttgcccag ctgtccggtt gcatttgcga agtactctcc gcaaccgttg
     9301 attcaaacag ttcgccgggc aaatcctgaa tcaacacttc ctgcccggcg gccatcaccg
     9361 ttagccagcg gcaggtgttt tccagctggc gcacgttgcc tggccacgcc agacgcgtca
     9421 gagcagcttc ggtttccgga tgcagtaact tcgcttctac gcccagttcg cgcgcggcaa
     9481 cctgtaaaaa atggcgcgcc agacggggaa tatcttcccg acgttcgcgc agcggcggca
     9541 gatgaacgcg gataacgttc aggcggtgga acagatcctc acggaactta ccttcctgca
     9601 ctcgctgttc gagattctgg tgagtggcag cgataatacg cacatccact ttcaccggcg
     9661 catagccgcc aacgcggtaa aactgaccgt ctgccagcac gcgcagcaaa cgcgtctgca
     9721 catccagcgg catatcacca atttcgtcga ggaataatgt accgccatcg gcctgttcaa
     9781 aacgcccctg acgaatggta ttcgcgccag taaacgcgcc tttctcgtgg ccaaacagtt
     9841 ctgattcgat caaatctttt gggatagctg ccatattcag cgcgataaac ggcgctttgg
     9901 cgcgcggact gtggcgatgc agggcatgag cgaccagttc tttaccggtg ccggattcgc
     9961 cgttaatcag cacgctaata gaagaacgcg aaagccgacc gataatacgg aacacgtcct
    10021 gcatggctgg cgcttcgccg atgatatcgg tcgttgggcc gttaagctga atattacgcg
    10081 gctgctgctg ttcctggtaa tgactgatag cgcgctcaac cagcgccact gcttcgtcga
    10141 tatcaaacgg tttgggcaga taatcaaacg ccccttgttg ataggcgctg acggcagcat
    10201 ccagatcgga atgtgcggtc ataatgatga ccggaagcat tggatggcgc tgtttaatct
    10261 gcttgagcag cgccagcccg tccattcccg gcatacggat atctgaaagc agcacatccg
    10321 gcgttttgct cgccagcgcc tccagtactt ccgcgccgtt ctcaaatgtc gtacaggtta
    10381 aacccgctcc agcgagcgca cgttcaagca cccaacggat ggaactatcg tcatcgacta
    10441 cccagactat ccctcgttgc ataaacgtca cctttatttc ctgataggca ggtaaaccga
    10501 gaactcggta tgacctggcc aactggtaaa ttcaattttg cctgaatgct gatcaatcaa
    10561 attacgggcg atggataagc caagcccggt gccaccttcg cggccgctga ccatcgggta
    10621 aaacagcgta tcctgcaaat gaggcggaat gcccggcccg ttatcttcca catcaatccg
    10681 cgccgccagc cggtagcgct cgccgtgtaa ggtcagttga aacgcggtgc gggtacgcag
    10741 aatgatttca ccgccttccg gccccagcgc ctgtagcgca ttgcgcacaa tattcagcaa
    10801 gacctgttca atttgatccg ggtcgtgcgc cagttccggc aggctggggt cgtaatcacg
    10861 aatcaaccgc acgttgtccg gcagttccat cgacaccagc gtcaccacgc gttcagctac
    10921 tttgtgaata ctttcggtaa cgcgcgtacc gggcagctgc ggccccaaca gacggtcgac
    10981 cagatttcgc agccggtccg cctgttcgat aatcactttg gtatattcga gtagtgatgg
    11041 gtcaggtaac gctttgctga gcagctgcgc cgcgccacgt aaacctccaa gcggattttt
    11101 aatctcatgt gccaggccgc gcactaaatc acgggcagca acctgctggg cgtgctgtag
    11161 ctgttcctga cttaagcggc gctggttatc catcggagcc atctccagca ggatcatgcc
    11221 gtccggcata cgctgggccg tcacagaaag gatatgcgag cgcccgtcga tgaccagcgt
    11281 cacttcgtta tcggtaaaac cttgccccgc ctccagactt tcttgcatca gctcgatatt
    11341 taatgagaag tagctcaaca gttccggtaa cggtgtacca aacaatttgc gggagctttg
    11401 ggcgagcagt tgttgcgcgg cggggttggc gtaatggatc gccagattgt catcaattaa
    11461 caaaatactg ttaatcagcg agttgaggat ctgcccagca tcgggctgcg tgcctgttgc
    11521 cataaagcag tctcctgaac aggttgcacc attttagtgc attatagctt tttacggata
    11581 aaaagcgcga agcatcagag aattgacgga gaaaaaagcc catgcagaga tgggctgaaa
    11641 gtttccacgg caactaaatc ccggcgttgt tgcgccgggt agtacaactt caactattag
    11701 acgctgtagt acagctcaaa ctctaccgga tgcggagtca tacgcacgcg gtcatcttct
    11761 tcgcgacgta gagcgatgta cgcatcaatt gcttcgtcag tgaacacgcc accggctttc
    11821 aggaactcgc ggtccagatc cagttcgttc agtgcttctt ccagagagcc tgcaacctgt
    11881 gggatctctt tcgcttcttc tggcggcagg tcatacaggt ttttgtccat ggcttcgccc
    11941 ggatggatct tgttcttgat accatcaaga ccggccatca gcagggcagc aaagcacagg
    12001 tacgggttag ctgccggatc cgggaaacgt acttcgatac gacgtgcttt cggagaagaa
    12061 accaccggaa tacggataga cgcagaacgg ttacgcgcag agtaagccag cattaccggt
    12121 gcttcatagc ccgggaccag acgcttataa gagttggtgg tcgggtttgc cagggcgtta
    12181 atcgctttag cgtgtttgat tacgccgcca atgtagtaca gcgcctgctc agacagacct
    12241 gcgtatttgt cgcctgcgaa caggttaacg ccgtttttag acagagacat gtggcagtgc
    12301 ataccggagc cgttatcacc gaacatcggt tttggcataa aggtcgcggt tttaccgaag
    12361 cggtgcgcta cgttgtgcac aacatatttg tagatctgaa tttcgtcagc ttttttggtc
    12421 atagtattga agcgggtagc cacttcgttc tgaccagcag tcgctacttc gtggtgatgg
    12481 gcttcaacca ccagacccat ctgttccatc accagacaca tttcagaacg aatatcctga
    12541 gccgagtcta ccggtggaac cgggaagtaa ccgcctttca ctgccggacg gtgacctttg
    12601 ttaccacctt cgtattgggt ggaggagttc catgcgcctt cgatatcgtc gatagcaacg
    12661 tgggaaccgg agatagatga tccgaaacgg atgtcatcga acaggaagaa ttcaggttct
    12721 ggcccgaaca gtacggtgtc ggcaatgcca gtggaacgca ggtaatcttc ggcgcgcttc
    12781 gcaatggagc gcgggtcacg gtcatagcct tgcagggtgc caggttcaag gatgtcgcaa
    12841 cggataatca gggtggagtc ggcgaagaac gggtcaatca ctgcggtgga tgcgtctggc
    12901 atcagcacca tgtcggactc gttaatgcct ttccagccgc caatcgagga gccgtcaaac
    12961 attttgcctt cttcgaagaa ttcagcattc acctgatgag cagggatagt gacgtgctgt
    13021 tctttacctt tagtatcggt gaagcgcaaa tcaacaaact tcacttcgtg ctcgttcagc
    13081 atcgtcagta cgtgttcagc ggacatactt taactctcct ggattggtca tggtcgtcgt
    13141 ggtaacgaaa tctgcaatta ttttggccgt gtcgccgtaa aaaagataaa gcgaaatctg
    13201 tgccaacttt taaattgccc ctaaaaggcg ttatcatgcg caccatcgtg caaaagggct
    13261 gcaccacgat gtgaatgttg caccaatata gtgcttcaat ggaaacatta agcaccatgt
    13321 tggtgcaatg acctttggat aacccttttt atgctccgtg aaagcgatca caaagggact
    13381 ctgcaatact tgtttgcgga ggatgtttgt gatcctgttt tgtagtgcga ttaatccgtg
    13441 tacaataacg cgctatttct aatgcctgag gcaaagttgt gatcgaaaaa ttgcgtaata
    13501 tcgccatcat cgcgcacgta gaccatggta aaaccaccct ggtagacaag ctgctccaac
    13561 aatccggtac gttcgactct cgtgccgaaa cccaagagcg cgtgatggac tccaacgatt
    13621 tggagaaaga gcgtgggatt accatcctcg cgaaaaacac cgctatcaaa tggaatgatt
    13681 accgtatcaa catcgttgat accccggggc acgccgactt cggtggtgaa gttgaacgtg
    13741 taatgtccat ggtagactca gtgctgctgg tggttgacgc atttgacggc ccgatgccgc
    13801 aaacgcgctt cgtaaccaaa aaagcgtttg cttacggcct gaagccgatt gttgttatca
    13861 acaaagttga ccgccctggc gcgcgtcctg attgggttgt ggatcaggta ttcgatctgt
    13921 tcgttaacct cgacgcgacc gacgagcagc tggacttccc gatcgtttac gcttctgcgc
    13981 tgaacggtat cgcgggtctg gaccacgaag atatggcgga agacatgacc ccgctgtacc
    14041 aggcgattgt tgaccacgtt cctgcgccgg acgttgacct tgacggtccg ttccagatgc
    14101 agatttctca gctcgattac aacagctatg ttggcgttat cggcattggc cgcatcaagc
    14161 gcggtaaagt gaagccgaac cagcaggtca ctatcatcga tagcgaaggc aaaacccgca
    14221 acgcgaaagt cggtaaagtg ctgggccacc tcggtctgga acgtatcgaa accgatctgg
    14281 cggaagctgg cgatatcgtg gcgatcacgg gccttggcga actgaacatt tctgacaccg
    14341 tttgcgacac gcaaaacgtt gaagcgctgc cggcactctc cgttgatgag ccgaccgttt
    14401 ctatgttctt ctgcgttaac acctcgccgt tctgcggtaa agaaggtaag ttcgtaacgt
    14461 ctcgtcagat cctggatcgt ctgaacaaag aactggtaca caacgttgcg ctgcgcgtag
    14521 aagaaaccga agacgccgat gcgttccgcg tttctggtcg tggcgaactg cacctgtctg
    14581 ttctgatcga aaacatgcgt cgtgaaggtt tcgaactggc ggtatcccgt ccaaaagtaa
    14641 tcttccgtga aatcgacggt cgtaaacaag agccgtatga aaacgtgact ctggacgttg
    14701 aagaacagca tcagggttct gtaatgcagg cgctgggcga acgtaaaggc gacctgaaaa
    14761 acatgaatcc agacggtaaa ggccgcgtac gtctcgacta cgtgatccca agccgtggtc
    14821 tgattggctt ccgttctgag ttcatgacca tgacttccgg tactggtctg ctgtactcca
    14881 ccttcagcca ctacgacgac gtacgtccgg gtgaagtggg tcagcgtcag aacggcgtac
    14941 tgatctctaa cggtcagggt aaagcggtcg cgttcgcact gttcggtctg caggaccgcg
    15001 gtaagctgtt cctcggtcac ggtgcagaag tttacgaagg tcagattatc ggtattcaca
    15061 gccgctctaa cgacctgact gtaaactgcc tgaccggtaa gaaactgacc aacatgcgtg
    15121 cttccggtac tgacgaagct gttgttctgg ttccgcctat ccgcatgact ctggaacaag
    15181 ctctggagtt catcgatgat gacgaactgg tagaagtgac tccgacctct atccgtattc
    15241 gtaaacgtca cctgacggaa aacgatcgtc gtcgcgccaa ccgcgcaccg aaagacgatt
    15301 aatttcgttt tttagttata aaaaacctgc catcgct
//
LOCUS       DAZSBF010000091        15157 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZSBF010000091 DAZSBF010000000
VERSION     DAZSBF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.092"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHXWD_002234"
     CDS             <1..1015
                     /locus_tag="ACHXWD_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7621141.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHXWD_002235"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHXWD_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR7621142.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHXWD_002236"
     CDS             1797..1904
                     /locus_tag="ACHXWD_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR7621143.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHXWD_002237"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHXWD_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR7621144.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHXWD_002238"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHXWD_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR7621145.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHXWD_002239"
                     /pseudo
     CDS             complement(3540..4696)
                     /gene="yehY"
                     /locus_tag="ACHXWD_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHXWD_002240"
     CDS             complement(4703..5620)
                     /gene="osmF"
                     /locus_tag="ACHXWD_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR7621146.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHXWD_002241"
     CDS             complement(5872..8169)
                     /gene="bglX"
                     /locus_tag="ACHXWD_002241"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR7621147.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8365..10080
                     /gene="dld"
                     /locus_tag="ACHXWD_002242"
     CDS             8365..10080
                     /gene="dld"
                     /locus_tag="ACHXWD_002242"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR7621148.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHXWD_002243"
     CDS             complement(10119..11051)
                     /gene="pbpG"
                     /locus_tag="ACHXWD_002243"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR7621149.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMLVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11225..11812)
                     /locus_tag="ACHXWD_002244"
     CDS             complement(11225..11812)
                     /locus_tag="ACHXWD_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR7621150.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11982..12560
                     /locus_tag="ACHXWD_002245"
     CDS             11982..12560
                     /locus_tag="ACHXWD_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR7621151.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12690..13451)
                     /locus_tag="ACHXWD_002246"
     CDS             complement(12690..13451)
                     /locus_tag="ACHXWD_002246"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7621152.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHXWD_002247"
     CDS             complement(13504..14940)
                     /gene="mdtQ"
                     /locus_tag="ACHXWD_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR7621153.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3697 a         4142 c         3800 g         3518 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgcagtg
     3781 gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg gcgctctcca
     3841 gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc ggcagcaagg
     3901 catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg ccgagcgtcc
     3961 ccagccacgg aaaggccgta accagcgcgg caagcggcgc aatcaacagg ccaaagagcg
     4021 ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc tgccgagcag
     4081 tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc agcaccgcac
     4141 caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga cggttggcgt
     4201 attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac agcggaataa
     4261 tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt cggcggatgg
     4321 catcactaca ggccagcaat gccagcgccg cggccagcca gaaaccactg ccgaggctgg
     4381 tacgcgccag cgcactgcca ttttgcgcca gttgggtcgc cgcctttcct gctccccaca
     4441 ccagcaatac gaagacgaat tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg
     4501 gaataaaaca ggctgtcagc caggcgcaac caacgcccac cagcatccag atcgtttgcg
     4561 gccacagttg ccagagatga cgcccttcac ccgagactaa acgattaggc gcgtaactga
     4621 taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga acaggattaa
     4681 tacgtagata agtcacggga aattacttcg tccacccttt ttgtttcagg tagtcggcag
     4741 ccactttttt ggcatccagt ccttccactg caatgctggc attcagttgc tgcaatgttt
     4801 ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga tactctttca
     4861 gcaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacgcct ttcggatcgc
     4921 ttaaggtttg cagccccagc gccgcgaccg gaccgtcagt gccgtaagcc attgcggcat
     4981 taacgccaga agtttgctgg gcagcggctt taatcgttac cgccgtgtcg ccgccagcca
     5041 gtgacagtaa ctgatcctga tcgagtttaa agtcgtaggc tttttcaaac gcgggtaacg
     5101 catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg ccctctttca
     5161 gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca tcctgacgca
     5221 cggcgatggt ccaggtgtta ttcgctggtg cgggcgtcag ccagattaac ttgttttgct
     5281 ctgcatcgag ttttttgact ttctcgtaac cttgctgcgc gtttttccat gccgcatcgt
     5341 tttcatcttt aaagaagaaa gcgccattac cggtatattc cggatagata tccagttcac
     5401 cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta ttcaccgtcg
     5461 gtacgccgtg gctttccagc acctgcaaga tgatgttgcc gagtagcgcg ccttcggtat
     5521 cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc acggctgcca
     5581 acataaccag tgaacctgcc cagacctttg agagtggcat gatgctttcc tcattctttt
     5641 actgttgttt tcagcgaatt aagagaaaag catagttgat aatgacagag ttagcctgag
     5701 cggaaggatt cagttgcaga atcagataaa tacttaaagg gcatattttg cccggcgttt
     5761 cttgccggat gcggcgtaaa cgccttatcc ggcctacaaa ggtcgaaaat tcagcaagtt
     5821 aagatatgaa catcaggcaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa
     5881 ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaagacat tgaacttgcc
     5941 aggttcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcga tatcaatcgg
     6001 gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg
     6061 cttcactgga cggctcatgg aagccgtcac atcctgcaag tacatctgta ctaccgtggc
     6121 cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcg gtcactttgc cgtcacgctt
     6181 catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtatagc tcagaccata
     6241 gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg
     6301 cttgtcggca ttatacggac gaccggtatt cagatggctg tagtacaccg ggatctgccc
     6361 gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac
     6421 atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc
     6481 ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg
     6541 tttaccggtg gctttcagcg ccgtaatcaa gtcacgttgg ctttgcggaa tagtgatatc
     6601 ggtacggctg gaggcctcgt gcgccatccc ctgtgcttca ccgaccacag ccaccaccac
     6661 atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg agcgcggatc
     6721 gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac
     6781 gttcgcccct ttggcataca gcactttacc gttttcaccg acggcatttt taatcccggt
     6841 cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg
     6901 tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt
     6961 ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg
     7021 gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct
     7081 gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc
     7141 gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat acttcgagta
     7201 atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc
     7261 cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt
     7321 gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt
     7381 ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag
     7441 ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt
     7501 gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat
     7561 caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat
     7621 tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc
     7681 gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata
     7741 agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag
     7801 gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat
     7861 tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac
     7921 ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg
     7981 gttatccgga ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt
     8041 cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa
     8101 taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag
     8161 ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa
     8221 accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa
     8281 ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt
     8341 tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt
     8401 ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg
     8461 gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct
     8521 ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt
     8581 ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat
     8641 gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag
     8701 ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa
     8761 ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc atcggtcatc
     8821 ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa
     8881 atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg
     8941 attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa
     9001 gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt
     9061 cgtgatattg aagccgacac gcccgcacgt tataacgccg atcctgatcg gttatttgaa
     9121 tcttctggtt gcgccgggaa gctggcggtc tttgctgtac gtctagatac cttcgaagcg
     9181 gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc
     9241 cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatacaccgg
     9301 gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta
     9361 ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag
     9421 aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg
     9481 ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg
     9541 ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggctggt ggattatttc
     9601 aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta
     9661 caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc
     9721 gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat
     9781 ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc
     9841 tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttgaaagaa
     9901 cagatgctgg aactgctaca gcagcgtggc gcgcaatacc ctgccgagca taacgtcggt
     9961 catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga tccgaccaac
    10021 agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga agtggagtaa
    10081 aaattacgga tggcatagta tcgccatccg tcattcactt aatcgttctg tgccgtctgc
    10141 cccgccgccg ccatttgggc ggctttttgt tttttatagc tcaacgctgc tgctggaaca
    10201 agcatcactt taccagtttc aatccaggta cgcaggcggc tggcatcggc aaaatgggta
    10261 tatttgccaa acgcatccat cactaccagc gctaccggtt tattattgat aaccgtacgc
    10321 atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg aatattccag
    10381 ttatcgcgat acaccagatg gttagtattg cggaacggca gcgtatacgt cgggttagag
    10441 aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata ttgtttgctg
    10501 gcaatgagca gtttggttaa gtcacgggca gttgaaacgt tatgcaccga caatccggta
    10561 ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt cattgcctta
    10621 ataaaggctt tgtaaccacc gggataatgg tgtgcaaggc ttgccgccgc gcggttttct
    10681 gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctatt cagtcgtacg
    10741 cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag tttttcatcc
    10801 agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat agacgcaatc
    10861 ggacgcacca gatccgggtg gttcgagtag atcactttgt tggtattcag atcaacaatc
    10921 atcgcgctac cggaggcaat ttcaggttgt gaagcggtgg tagcggctgc cgttttcgca
    10981 acggcctgcg gtgccaaagg cacagccagc atcagggcca ggctaaataa agaaactcga
    11041 aatttcggca tgatgagcat tcagatagtg gttcacgcgc acaggttgcg caccgccgga
    11101 gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg atcgtcaaag
    11161 gagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcaa cgtcaaagaa
    11221 ctttctagaa caaacgataa ccgtagcccc atagtataac ggttagggcg agcagcactt
    11281 ccagtaccag cacgccaatc gccagcgtcg aactagagaa gctaaggcct tcctctttgt
    11341 tgatattcag gaagctcgga ataccaaggt aaagcaggta gccggtgtaa aacagcgcca
    11401 ccgtgccgac cagcgcacac aaccagacca gtggataaag cgccaccaga ccgcttaaaa
    11461 acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac ggacgctgcg
    11521 gataattacg cgccatccac cagatgaccc gccccattac cgcaacccca gccagcataa
    11581 cgccataaaa caggacagcc agcgccagtc cggtaaacca ggataacttc aggatagtgc
    11641 catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc accggaatcg
    11701 ccgccatcag caaaacgtgg tgggtgtaat ggtgagaaat cgtttcgttt tcgcgattaa
    11761 tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc ataccgcccc
    11821 cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga ttattttttg
    11881 ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa tgatttccag
    11941 ggtgtatgct tacaggcaac caaagggaga cagactggcc tatggatctc aatacactca
    12001 tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt gaaaccgtga
    12061 ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg gtggtacttt
    12121 ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctgtgcggg cggcggtttg
    12181 gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg gcgcagaaac
    12241 ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ctttatgtat ggctttcggg
    12301 tgattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt ctgccgctga
    12361 atattctcgg cgcatttgcc tgggcgttga tttttaccac tattggttac gctggtggtc
    12421 aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg gtctggttga
    12481 ttctggtcgt ggttctggtg gtgggcgtgc gctggtggct gaaacggcgc gggaagaaaa
    12541 agccggatca tcaggcgtaa aaccattgcc ctgggtaagg cgttcgcgcc gcatccgaca
    12601 tccggtgcac catgcctgat gcgacgcttg tgcgtcttat catgcctacc aaaattttac
    12661 ccattgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcgccaac
    12721 ataaagccgc catccactat caacgactgc ccggtggtgt aatttgcgcc ctctgagcac
    12781 agccacgcta ccaggctggc aatctcatgc gttgcgccaa aacgccgcag aggaatcgaa
    12841 ggctccgcgt cgggcttcac gtcgctgtca tccatgccat tcattggcgt ggcgatcgcc
    12901 ccaggcgcga ctgcgttcac caaaatctta tgcctgacca gctccagcgc catcgttttg
    12961 gttaacccgc cgagcgcatg tttagcggct gtgtaagcgc tggcatccgg cagcggcgta
    13021 tgttcatgta ccgacgtaat gttgatgata cgaccgccct gcccttgttt caccatctgg
    13081 cgggccgcaa tttgtgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    13141 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    13201 agcacatcaa tacgccccag ccgttgaatg agtttctcca gcgcctgcgc accttctggc
    13261 agtttaccga gctccagctg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    13321 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    13381 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aataatcgca
    13441 acctgtgcca tcaagttctc cacttaacgc tgaataaacg ttaagtatag aaggcgcata
    13501 tcatcagcgt ttgtaacccc cgcccaacgc accagtgagt tgaatagaag catccagcca
    13561 ctgcccttgc aataacaggc cattggcccg ctcacgcagc gcggggattc tggcttcgct
    13621 gacgcgggaa ccaacaatga tgcccgcgtt aaagcgcgcc tgcgcaagac ctaccacacg
    13681 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgcaagtg tctgaacctg
    13741 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    13801 agacaagttg ctttcggctt ttgcgatatt gagattggcg ttaagacgac cactatcgaa
    13861 aatgggtagc gtcaggcctg ccgtaacgcc catttgctgc gcggaatgac ggaacagatc
    13921 gcttaagtgc aacgcatcct gttgcaggaa ggccatcagg ttgatgtcag gataaaatgc
    13981 cgcttttgcc gcatcaatgg tgcttagcga tgactcaacg taccagtgcg ccgcctgcaa
    14041 atctgcccgc cgggccagta aggagtaccc cagttcatca ggaagctggc ttgccacttt
    14101 cggcaacgcg accggtttaa gcttcaatga ctttgtctgg ttatttgtaa gtgcgcttaa
    14161 ccgtgcctca ataattttca ttttccccgc gacatcgttg agctgctgcc gggttttgct
    14221 ggcattaata tcggtttcca caccttcaac tgaagaagta atcccgttct gatatagctg
    14281 gcgatcggtc gcgataatgg tgttctgctc tttttctatt tgctgcaaga ccgtgtttaa
    14341 cgccgcctgg gtttgccact cccagtacag gcgggctacg ctgccagcca gcaattggcg
    14401 ggtttgctcg cgttccgccg cccgtgcttt aaccgtaccc aggcgggcag taacctccgc
    14461 ccgattcttt ccccagatat cgagatgcca gcccgccgtt aagccaaaag taccgttggt
    14521 gtaccacggg ccggtcgtac ctgcggccgg atcgttcaga gcaaacggcc ccattaagcc
    14581 ttctgccgac attttttgcc gctccatatc cgccgaaaag tcgatctgcg gaccatcctg
    14641 agtggcaact gccttcgcct gggcttcagc tagctgaatg cgctgttcag ccacctgcat
    14701 atccggtgcg ttctgtagtg cattgttaat taatgaagtg agctgattat cgtgatactc
    14761 cagccaccat tggctgtctg gccaaccatt tttcagcgcc gtgggtaatg cggtgtcaac
    14821 ttgtgcagcg ggcgtttgct ggcttaacgc ctggcgggtt tcatgcatag gcgcacaccc
    14881 ggccagcatc agtaacagcg gaaaacaggc gatggctgga taaaaggaat cacgattcat
    14941 gggggaataa tcaggtaaga aacggtgcgc ggagattacc gtgtgttgcg atatattttt
    15001 tagtttcgcg tggcaataca tcagtagcaa taaaacgaca tacccagaaa aatatagact
    15061 aagtaaatga tatcttccga tttatcttaa tcgtttatgg ataacggcaa agggcttcgt
    15121 tttttcctat acttattcag cactcacaaa taaagga
//
LOCUS       DAZSBF010000092        15084 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZSBF010000092 DAZSBF010000000
VERSION     DAZSBF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.093"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHXWD_002248"
     CDS             <1..781
                     /gene="yjeJ"
                     /locus_tag="ACHXWD_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR7621154.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="ACHXWD_002249"
     CDS             complement(984..1337)
                     /locus_tag="ACHXWD_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR7621155.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHXWD_002250"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="ACHXWD_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR7621156.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="ACHXWD_002251"
     CDS             complement(3165..3458)
                     /locus_tag="ACHXWD_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR7621157.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHXWD_002252"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="ACHXWD_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR7621158.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /locus_tag="ACHXWD_002253"
     CDS             complement(5012..5482)
                     /locus_tag="ACHXWD_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR7621159.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="ACHXWD_002254"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="ACHXWD_002254"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR7621160.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHXWD_002255"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="ACHXWD_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR7621161.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="ACHXWD_002256"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="ACHXWD_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR7621162.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHXWD_002257"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="ACHXWD_002257"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR7621163.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="ACHXWD_002258"
     CDS             10838..11413
                     /locus_tag="ACHXWD_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7621164.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="ACHXWD_002259"
     tRNA            11520..11595
                     /locus_tag="ACHXWD_002259"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            11838..11952
                     /locus_tag="ACHXWD_002260"
                     /pseudo
     CDS             11838..11952
                     /locus_tag="ACHXWD_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12212..12511
                     /locus_tag="ACHXWD_002261"
     CDS             12212..12511
                     /locus_tag="ACHXWD_002261"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR7621165.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHXWD_002262"
     CDS             complement(12502..12627)
                     /gene="laoB"
                     /locus_tag="ACHXWD_002262"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR7621166.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12694..13749
                     /gene="cadC"
                     /locus_tag="ACHXWD_002263"
     CDS             12694..13749
                     /gene="cadC"
                     /locus_tag="ACHXWD_002263"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR7621167.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHXWD_002264"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="ACHXWD_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR7621168.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAZSBF010000093        14965 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZSBF010000093 DAZSBF010000000
VERSION     DAZSBF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.094"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHXWD_002265"
     CDS             <1..196
                     /locus_tag="ACHXWD_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621169.1"
                     /translation="KAILTDGLFALAQKKMPDATLRVLYSHICQIQQRIRFPQLAHFH
                     TCPPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHXWD_002266"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHXWD_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR7621170.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="ACHXWD_002267"
     CDS             complement(1608..1931)
                     /locus_tag="ACHXWD_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR7621171.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHXWD_002268"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHXWD_002268"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR7621172.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHXWD_002269"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHXWD_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR7621173.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHXWD_002270"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHXWD_002270"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR7621174.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHXWD_002271"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHXWD_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR7621175.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="ACHXWD_002272"
     CDS             7531..8568
                     /locus_tag="ACHXWD_002272"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR7621176.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHXWD_002273"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHXWD_002273"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR7621177.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHXWD_002274"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHXWD_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR7621178.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHXWD_002275"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHXWD_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR7621179.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /locus_tag="ACHXWD_002276"
     CDS             10739..11620
                     /locus_tag="ACHXWD_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7621180.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHXWD_002277"
     CDS             complement(11829..13163)
                     /gene="srmB"
                     /locus_tag="ACHXWD_002277"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR7621181.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13295..14032
                     /gene="trmN"
                     /locus_tag="ACHXWD_002278"
     CDS             13295..14032
                     /gene="trmN"
                     /locus_tag="ACHXWD_002278"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR7621182.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14017..>14965)
                     /locus_tag="ACHXWD_002279"
     CDS             complement(14017..>14965)
                     /locus_tag="ACHXWD_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR7621183.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3612 a         3785 c         3921 g         3647 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgccc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccaca aattgtagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa tgaaccacaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc ttataagtat
    11401 cgaaagcatc aagcgttgtg tcgcggcgaa tatcggagtc agctacctgc cgcgttttgc
    11461 ggtggcaaaa gagttggagt gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagccacgta ggcctgataa
    11761 gcgtagcgca tcaggcagtt ttgcgtttgc cagcactttt aataccaggc ttaaacccgg
    11821 cattgagatt actcttctgt cgtttgcggt ggcacgcccg ttccgcttgg tttacggcgc
    11881 ttaccaatat ttttggtgtc gcgatggcgt tttttcaccc gcggtttctc tttctcttta
    11941 gcttttttct tctcggcgcg ttttgccagt actttcttcg atggcttgcc ggtctgcttc
    12001 tcgcttggcg cacgcgtttt cgggcgtaac tcatcaataa cgcgagcttt aatcggctct
    12061 tcaatgtaac ggccaacctt gcccagcagc aaatggtcat gggcttccac cagcgaaatt
    12121 gcggtgcctt tacgaccggc gcgcgcggta cgtccgatac ggtgcaaata agtatcgcca
    12181 ctgcgcggca tatcgaagtt aaagacgtgg ctgacgtcag gaatgtcgat accgcgcgcg
    12241 gcaacatcgg ttgcaaccag tacgtttacg cgaccttcgg tcaaacgctt gatcgcttcg
    12301 ttacgcttgc cttgtaccat ctcaccttcg agatagcagt tgttgatgcc cgcttcgcgc
    12361 agccagtttg ccagctcatg cacacgttca cgcttacgca caaacacaat tgagcgggtc
    12421 gcttccggct gttttaacag atgcaccagc aatgcggttt tatgttccag gtcatcggcg
    12481 cggtaatacc actgatgaat ttttttgcgc tcacgggtgg acggattggc agaaacctcc
    12541 accggatctt ccagcagacg ctcggcaaag tcctgaatcg catcgccttc cagcgttgcc
    12601 gaaaagagca gggtctgttt acgccagcgc gtttcgccag caatatgttc gatatcctga
    12661 gcgaagccca tatccagcat acggtctgct tcgtcgagga tcagcgtttc aaccgcgcgg
    12721 caatcgaagt tctcttcttt tatgtattgc agcagacgtc cggtcgtggc gaccacgatg
    12781 tcctgatttt cgctgaacac ttccgcgtgg ttcatatagg ctacgccgcc ggtgatggtg
    12841 gcgatatcca gatgcgtatg tttcgccagt tcgcgggcat gatcggccac ctgcatcgcc
    12901 agctcgcgag ttggggtgag gatcaaaata cgcggcggac cggatttctt acgtgggaaa
    12961 tcgagcaggt gctgcaacgc tggcagcaga tacgccgccg ttttaccggt gcctgtcggc
    13021 gcagaaccga gtacatcacg gccatcgagc gcaggcggaa tggcggcagc ctgaatggcg
    13081 gtcgggcgag tgaaaccttt atcctggagg gcttccagca ggctttcgtc gagttcaagt
    13141 tcggaaaaag tcgttacagt catgttctac ctctgtgtgg ggcgctgatt atagacgtta
    13201 cggctgcaat cttcatctgt ttgtatggat atcgcttctc gggtattgtt ctcacccggt
    13261 gctatcgccg tttttcctgc aaggttttat tttcatgtca cagtctacat ccgtgcttcg
    13321 tcgtaatgga tttactttta aacagttttt tgttgctcac gatcgctgtg cgatgaaagt
    13381 gggaacggat ggcattttgc tgggcgcatg ggcaccggtg gctggggtga aacgttgcct
    13441 tgatatcggc gcgggtagcg ggttgctggc actgatgctg gcgcagcgaa ccagtgacag
    13501 cgtgataatt gatgccgttg agctggaaag tgaagctgcc gcgcaggcgc aggaaaatat
    13561 caaccagtcc ccgtgggcag agcggattaa cgtccatacg gcggatattc agcagtggct
    13621 cacacagcag acagtacgct tcgatttaat catcagtaac ccaccttact atcagcaggg
    13681 agtagagtgc gcgacacctc aacgggaaca ggctcgctat accactacgc ttgatcaccc
    13741 atcgctgctg acctgtgcgg cggagtgcat taccgaagag ggatttttct gcgtggtgct
    13801 gccagagcag gttggtaatg gttttactga actggcatta agcatgggct ggcatttacg
    13861 tttgcggacg gatgtggcgg aaaacgaagc gcgactgccg catcgggtgc tgctggcatt
    13921 ctccccgcag gcgggagaat gctttagcga tcgcttagtc attcgtgggc cagaccagaa
    13981 ctattccgaa gcgtatacgg cgctgaccca ggctttttat ctgtttatgt aatgattgcc
    14041 ggggggaagg atcgatggac cggaatgcgt gagcagttcc gggtaatcca gcgtaaaatg
    14101 caggccacga ctctccttgc gcatcatagc acagcgaaca atcaactcgg caacctgtac
    14161 cagattacgc agctccagca aattattcga cacgcggaag tgggcgtaat attcgtctat
    14221 ttcctgttgc aacatattga tacgccgcag ggcgcgctca agacgttttg ttgtgcgcac
    14281 aatgccgacg taatcccaca taaacagccg tagctcgtgc cagttatgct gaattactac
    14341 ccgttcgtca gggttctcaa cgcggctttc atcccacggt ggtaacgtac tgacgccgtg
    14401 ggcatcaggc atacgtctgg tgatatcttc tgccgccgac cagccataga ccagacactc
    14461 cagcaatgaa ttcgaggcca tgcggttagc gccgtgtaag ccggtataac tcacctcgcc
    14521 aatggcatac aagccctcga cgtccgtacg cccatgatca tcaaccatta caccaccgca
    14581 ggtatagtgt gcagcaggca caatcggtac cggttcttgt gtgagatcaa tccccagccc
    14641 gagcaatttt tcataaatca tcgggaaatg ctggcgaata aaatcggcgg gcttatggct
    14701 gatgtcgagg aacatacaat ctgcgccgag gcgtttcatt tcatggtcaa tggcgcgggc
    14761 gacaatatcg cgcggggcca gttcgccgcg ctcatcaaaa tcgggcataa aacgcgtgcc
    14821 atccgggcgc ttgagataag cgccttcgcc gcgcagtgct tccgttaaca ggaaattgcg
    14881 tgcctgtggg tgatacagtg cggtaggggg gaactgatta aattcgagat tggcaacccg
    14941 actgaggtgt actggcaata gcgga
//
LOCUS       DAZSBF010000094        14848 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZSBF010000094 DAZSBF010000000
VERSION     DAZSBF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.095"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHXWD_002280"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHXWD_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR7621184.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHXWD_002281"
     CDS             1598..4654
                     /locus_tag="ACHXWD_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR7621185.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHXWD_002282"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHXWD_002282"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR7621186.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHXWD_002283"
     CDS             5161..5349
                     /locus_tag="ACHXWD_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR7621187.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHXWD_002284"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHXWD_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR7621188.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHXWD_002285"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHXWD_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR7621189.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASELGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHXWD_002286"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHXWD_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR7621190.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHXWD_002287"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHXWD_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR7621191.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHXWD_002288"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHXWD_002288"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR7621192.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHXWD_002289"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHXWD_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR7621193.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHXWD_002290"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHXWD_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR7621194.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHXWD_002291"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHXWD_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR7621195.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHXWD_002292"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHXWD_002292"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR7621196.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHXWD_002293"
     CDS             complement(14591..14785)
                     /locus_tag="ACHXWD_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621197.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT         3709 a         3641 c         3952 g         3546 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 aatcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acttggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaa
//
LOCUS       DAZSBF010000095        14791 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZSBF010000095 DAZSBF010000000
VERSION     DAZSBF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.096"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            112..726
                     /gene="evgA"
                     /locus_tag="ACHXWD_002294"
     CDS             112..726
                     /gene="evgA"
                     /locus_tag="ACHXWD_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR7621198.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            731..4324
                     /gene="evgS"
                     /locus_tag="ACHXWD_002295"
     CDS             731..4324
                     /gene="evgS"
                     /locus_tag="ACHXWD_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR7621199.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4380..5525)
                     /gene="yfdE"
                     /locus_tag="ACHXWD_002296"
     CDS             complement(4380..5525)
                     /gene="yfdE"
                     /locus_tag="ACHXWD_002296"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR7621200.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5599..6543)
                     /gene="yfdV"
                     /locus_tag="ACHXWD_002297"
     CDS             complement(5599..6543)
                     /gene="yfdV"
                     /locus_tag="ACHXWD_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR7621201.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6613..8307)
                     /gene="oxc"
                     /locus_tag="ACHXWD_002298"
     CDS             complement(6613..8307)
                     /gene="oxc"
                     /locus_tag="ACHXWD_002298"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR7621202.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8361..9611)
                     /gene="frc"
                     /locus_tag="ACHXWD_002299"
     CDS             complement(8361..9611)
                     /gene="frc"
                     /locus_tag="ACHXWD_002299"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR7621203.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10124..10747)
                     /locus_tag="ACHXWD_002300"
     CDS             complement(10124..10747)
                     /locus_tag="ACHXWD_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR7621204.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11044..11319
                     /gene="ypdI"
                     /locus_tag="ACHXWD_002301"
     CDS             11044..11319
                     /gene="ypdI"
                     /locus_tag="ACHXWD_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR7621205.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(11396..11638)
                     /locus_tag="ACHXWD_002302"
     CDS             complement(11396..11638)
                     /locus_tag="ACHXWD_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR7621206.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            11991..12911
                     /gene="lpxP"
                     /locus_tag="ACHXWD_002303"
     CDS             11991..12911
                     /gene="lpxP"
                     /locus_tag="ACHXWD_002303"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR7621207.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13267..13338
                     /gene="ypdK"
                     /locus_tag="ACHXWD_002304"
     CDS             13267..13338
                     /gene="ypdK"
                     /locus_tag="ACHXWD_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR7621208.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13403..14641)
                     /gene="alaC"
                     /locus_tag="ACHXWD_002305"
     CDS             complement(13403..14641)
                     /gene="alaC"
                     /locus_tag="ACHXWD_002305"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR7621209.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         4205 a         3444 c         3140 g         4002 t
ORIGIN      
        1 atcttaaagg aagctcagat tttcttattt ttattgagaa aatgagatga cgccttatgt
       61 ctgtattact acagggagaa gggagatgct tcattgcaaa gggaataatc tatgaacgca
      121 ataattattg atgaccatcc tcttgctatc gcagcaattc gtaatttatt gatcaaaaac
      181 gatattgaaa tcttagcgga gttaactgaa ggcggaagcg ccgttcagcg ggtggaaaca
      241 cttaagcctg atatcgtcat cattgatgtc gatatccccg gagttaacgg tatcctggtg
      301 ttagaaacgc tgaggaagcg ccaatatagc ggaattatta ttatcgtctc cgctaaaaat
      361 gaccattttt acgggaaaca ttgtgctgat gctggcgcta atggtttcgt gagtaaaaaa
      421 gaaggcatga acaatatcat tgcggctatt gaagctgcaa aaaatggcta ctgctatttc
      481 cccttctctc tcaaccggtt tgttggaagt ttaacgtccg accagcaaaa actcgactcc
      541 ttatcgaaac aagaaattag tgtcatgcgg tatattcttg atggcaagga taataatgac
      601 attgcggaaa aaatgttcat cagcaacaaa actgtcagca cttataaaag tcgcctgatg
      661 gaaaaattag aatgtaaatc actgatggat ctttacacat tcgcacaacg taacaaaatc
      721 ggctaaccac atgaagtttt taccctatat ttttcttctc tgttgtggtc tttggtcgac
      781 cataagtttc gcagacgaag attacatcga atatcgtggc atcagtagta acaaccgtgt
      841 catacttgat ccagtacgtc tgagcaacaa ggaattacgt tggttagcga gcaaaaaaaa
      901 tcttgtgatt gcagtacata agtcccaaac ggctacgttg ttgcataccg attcgcagca
      961 acgagttcgt ggtattaatg ctgattattt aaatctttta aaaagaacgt taaatatcaa
     1021 attaacactc cgggaatacg ccgatcatca aaaagcaatg gacgcgctgg aagaaggtga
     1081 agtcgatata gtgttatcac atttagtagc ttcgccgcct cttaatgatg acattgctgc
     1141 aaccaaacca ctgataatta cctttccggc gctggtaacc acccttcacg attcaatgcg
     1201 accgcttacc tcatcaaaac cagtaaatat tgctcgagta gcaaattatc cccctgacga
     1261 ggtaattcat caatcatttc caaaagcaac aattatctct tttacaaatt tatatcaggc
     1321 attagcatcc gtctcagccg gacagaatga ttactttatt ggtagtaaca tcattaccag
     1381 cagtatgatt tcccgctatt tcactcactc cttaaatgta gtgaaatatt ataactcacc
     1441 gcgtcaatat aattttttct tgaccagaaa agaatctgtc gttcttaatg aagtactcaa
     1501 tcgatttgtt gatgctttga caaatgaagt tcgctatgaa gtatcacaaa attggcttga
     1561 tacaggaaac ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacaatg
     1621 gattaagcag catcccgatt taaaggtgct ggaaaatcct tactcccctc cctattctat
     1681 gacggatgaa aatggctcgg ttcggggcgt tatgggggat attcttaata ttattacttt
     1741 gcaaacaggt ttaaattttt ctccgatcac cgtttcacac aatatccatg ctggaacaca
     1801 gctgaatccc ggcggatggg atatattacc cggtgctatt tatagtgaag atagagaaaa
     1861 taatgtttta tttgctgaag ccttcataac aacgccttac gtttttgtca tgcaaaaagc
     1921 gcctgacagt gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga
     1981 gcttcattcg caattaaaag agatgtatcc ggaggtagag tggataaaag tcgataacgc
     2041 cagcgctgca tttcacaagg tcaaggaagg cgaacttgat gctctggtcg cgacacagtt
     2101 aaattcacgt tacatgatcg accattacta tcctaatgaa ctttatcatt ttcttattcc
     2161 cggcgttccg aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga
     2221 tattattaat aaagcactga atgcaatccc cccaagcgaa gttctgcgcc tgaccgaaaa
     2281 atggattaaa atgcccaatg tgaccattga cacatgggac ctctatagcg agcaatttta
     2341 tattgttacg acattatccg ttttattagt tggcagtagc cttttatggg gattttacct
     2401 gttacgctca gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt
     2461 ccgaaaagca ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa
     2521 tgtcattagt cataatagtg cttttgaaca ttatttcact gcggattact acaaaaatgc
     2581 aatgttacca ttagaaaaca gtgactcccc ctttaaagat gttttttcta atgcgcatga
     2641 agtcacagca gaaacgaaag aaaatcgaac aatatacaca caggtttttg aaattgataa
     2701 tggcatcgag aaaagatgca ttaatcactg gcatacatta tgcaatctgc cagccagcga
     2761 tcatgctgtt tatatttgtg gttggcaaga tattacagag acgcgtgatt taattcatgc
     2821 actcgaagta gagagaaata aagcgatcaa tgcaactgtc gcaaaaagtc agtttctggc
     2881 aacaatgagt cacgaaataa gaacaccaat aagctccatt atgggcttcc tggaacttct
     2941 gtcgggttct ggtcttagca aggagcaacg ggtggaggcg atttcacttg cctactccac
     3001 cggacaatca ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg
     3061 taactatcaa cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca
     3121 ctctttcggt gcgattgctg caagcaaatc gatcgcatta atttgcagca gtacatttcc
     3181 tgatcattac ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact
     3241 gagcaatgct ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctggttca
     3301 cattgatgac aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc
     3361 gcaggaagaa caacaacaac tctttaaacg ctacagccaa acaagtgcag gtcgccagca
     3421 aacaggatct ggcttaggct taatgatctg caaagaatta attaaaaata tgcagggcga
     3481 tttgtcatta gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga
     3541 aattagccag caagtggcga ctgtcgaggc aaaagcagaa caccccatca cactacctga
     3601 aaagttgagc atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca
     3661 gctaaatcta ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa
     3721 agtcagtatg caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg
     3781 ttttgagttg actcgcaaac tccgtgagca aaattcttcc ttacccatct gggggcttac
     3841 agccaacgca caggctaacg aacgtgaaaa agggttaagt tgcggcatga acttatgttt
     3901 gttcaaaccg ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc
     3961 gcatattgca cctcagtatc gccaccttga tatcgaagcc ctgaaaaata atacggcgaa
     4021 cgatctacaa ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct
     4081 acccgctgcg tttcaagcac tagaagctgg cgataacaga actttccatc agtgtattca
     4141 tcgcatccac ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca
     4201 gttagaaata acacctgttt cagatgacag taagcctgaa attcttcagt tgctgaactc
     4261 tgtaaaagaa cacattgcag agctggacca ggagattgct gttttctgtc agaaaaatga
     4321 ctaaatagcg gctcccacaa tgttctaatg tgggagctat ttaccagcac atcttacatt
     4381 tatgatgaga actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc
     4441 ataacatgcg ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattcct
     4501 ccggcttcaa tcaacatatt tctcgcctga gtttgtggca aattaatggc ctcagccaca
     4561 cttaataacg gcgcgacggg cacaccaact tcatgtattt tggctaacca aacttcagct
     4621 gcctgcgttt ttaacgttcg ctcaatatat tgtttaagaa tagcctggtt ttgtacgcgt
     4681 aaaatattgc tgctaaatcg gggatcatta accagttccg taagctccag tgcctggcat
     4741 aacgcagaaa aaagcttgtc attaccacaa caaatcgtaa tcggcttatc ctgagtatcg
     4801 aaaacatcaa aaggtgccat gtagggatgg cgatttccca ggcgttgtgg tgacttccct
     4861 gtcgcaatat atgccatcag accatgctcc agaaaactca gcgtggcatc aaacatcgct
     4921 atatcgacat gcgcccctct ctggctcttt tcgcggccat aaagtgcact cactattccg
     4981 ctgaataaat agacaccgcc gcatagatcc gcaagagagg taccaacacg cactggcgga
     5041 gcatcagggt accctgtttc catcattatc ccactcattg cctgaatgat ggtatcgtag
     5101 gcaggagcat cttttagcgg accggtatgt ccgaaacctg acgatgaagc atatatgagg
     5161 cgcgggttga tttcttgtaa cctttcccat gaaaacccca gtttttccat tgtacctggg
     5221 cgaaaattct cagctaatac atcagcttgt ttgagcatat ttataaatat acttttatcg
     5281 tgatcattct ttaaatcaag aaccacactc tctttgccat gattaataaa actgtaatag
     5341 agtgactgtc catccacata gggaccaaat gtgcgggtat catcaccatg acctggtggc
     5401 tcaactttaa ttacccttgc gcccatatta caaagaagtt gagttccgaa aggtccatta
     5461 aggacatgtg tcatatcgat aactaataag ccttcaaacg gccctttgct ttcattattt
     5521 gtcatttgct tatgccttga aaaaagccga aggtgaaaag acacttcggc atttatgtaa
     5581 aatcaataac atgattaatt atgaaaccag tcgactgaca taaatccaca agggagccgt
     5641 gacgacaaag cccagtacgc tcacagccaa tgacgctgta ccagtacggg tgtagacatt
     5701 aaaccggctg gcaataatga ttccggagaa tgccggcggt aatgcgcctg ccagtaccat
     5761 catctgcaaa tgttcgctgt tcaaatgaca tgccatacca acgagaagca gcgccagtgg
     5821 catcagaatc aacttcagga aggtgttata agcaatttcg gcactgaact cgaatttatg
     5881 tgcagccaga gtcaaccccg cagcgaatac cgctacccct gagttagcct tcgcaatcag
     5941 attaaaggtc gggtcccatg ctgccggaat ttttacccca accagcacca ggatcgttgc
     6001 cagaacaggt gcccatacta ctggctcctt tgccgcagaa attaatgcgc tcagattact
     6061 attcttctta ccatccgctc ctgaggaagg attcagcaaa tacagaccaa taggaatagt
     6121 aattgcgtta acaataatag aaataattgc taccactaaa cctgttgata cggaatcacc
     6181 ataaatagga tcgagaactg caaaccccag gaatccaatg gtaggtgaac ccgcaattaa
     6241 tgcacataca gcggcttctg catgggtacg tttaaaaaat ttgtagcaac caaaccaaga
     6301 gaaaaagaaa catccgacaa taaccacaag tgatacaagg gtcagacgag tgtccgcaaa
     6361 aatcatttcc ctgtttgccc gggtaataga aacaaataga gccgcaggaa gagcgtagtt
     6421 taataccagt ttattaaatg cgcgggcttg atcttctgaa aaagtttctc gtctgccgct
     6481 aaaataaccc aacagcatga ttacgataat aggcaataaa tcgccaataa aaaatgttag
     6541 cataatagta tttccgataa gggtcccctc accatcgcat aatgagttgg caagaggaga
     6601 cgatgtcagt atttaattac cagcgacttg ttttgggtta agtttggtaa tatggccact
     6661 ttcagttcct gcagcagggt cgatgaccac attaataatg gtcggtttgc gcgactgaat
     6721 accggtggtt aaagcatgac gaagttcatc tgtcgtggtg acgttatagc caacgccacg
     6781 aaacgcatcc attaatttgt cataccttgc atggtgcaac agatccgttg gtgatggtgc
     6841 accagcgcca ctgagatcaa caccgtctcc tctgtagatg ccgccattat taaaaataac
     6901 gatcgtcacc ggcaggttat atcgacaaat cgtttcaatt tccatcccac tgaaaccaaa
     6961 agcactatca ccttcaatgg cgacaaccgg agaaccagaa gtcacgctag caccgatggc
     7021 atagcccata ccgatgccca tgacacccca ggtaccacaa tccagacgac gacgtggttt
     7081 atacatatca ataatatttc gtgcattatc cagggtattt gcaccttcat taactaaata
     7141 aatatcctgg ttctcgcgca atacgtcgcg cacagcactt aatgcattaa agtaatttaa
     7201 tggttgagta tctgtactta atttttcatg cattttttgt gcattttgct gcttgtggat
     7261 atttaaaata tcgcgccata ccagtggagt cgtaaatgtg ttttgtttca gttctgccag
     7321 cataccttgc atactggatg caatatcacc aacgactggc acagcaatgg ggcggttgct
     7381 gtcaatttcc tgcggttcaa tatccagttg aataaactgt gtatctgccg cccatccttt
     7441 tttaccgtgt gccaataacc aattcagtcg tgcaccaaca agcatgacaa cgtcagcatt
     7501 tgccagggca aacgaacgcg cagctgccgc agaaagtgga tgcgtatctt caaggatccc
     7561 tttcgccata gacattggca ggaatggaat ctgagcactt tcaataaatt cacgaagctg
     7621 ttcatcagct tgtgaatacg ccgcgccttt gccaaggata attaatggcc gttcagcttt
     7681 tgctaaaagc gaaattgcgc tagtgactga cttcgggcat ggcaataatg ctggcgacgg
     7741 attttcaact ttaacaatcg tggttaacgc ttcgtctttt tccatcgtcg cggccaggac
     7801 atttgctggc aaatcaagat aaactccgcc agggcgaccc gatacagaga cccggatagc
     7861 gcgtgccaat gcaatgccaa gatcctgcgg ctgattaacg cgaaatgctg ctttggcata
     7921 cggttttgcc gcattcattt ggtccagctc ttcataatca ccttgctgta ggtcgacgat
     7981 cgcgcggtcg ctggagccgc taatcatgat catcggaaaa ccatttaccg ttgcgttggc
     8041 caatgcggtc aaaccattga ggaatcctgg cgcagaaact gtcaggcaga tccccggttt
     8101 ttgggtaaga aaaccgcttg ccgcagcggc atagcctgcc gactgctcat gacgaaaacc
     8161 aatataacga atgccttccg cctgggcatg gcgtgccata tccgtcacag gaatacctac
     8221 aacaccataa atagtgtcaa tattattctg ttttaatgct tcaacgatga tatgcatacc
     8281 atctgtcatt tgaagttgat ctgacatgat taataacctc gaaattttta taaattggga
     8341 gccagaagcc cccgttaata tcagatggcg tggttttgct tcattgcagc aatttcatcg
     8401 tcgctataac ccagctcctg caatacagca gcggtatgtt cacctaatag cggcgcagct
     8461 ttaatatccg gcgtaaaggc agagaatttc attggacaac caacggtcag atattttcca
     8521 cgcaacggtt gttccacttc aacaacactg ccactttggc gcaaagaggg atcaagtgaa
     8581 atttctttca tacttaaaac cggtgcacaa ggaatatcaa actgagtcaa ataggccacc
     8641 gcttcatgtt tatcaatagt gacagtgtat ttttcgattt cagcaaaaat atcgaaaata
     8701 tgtggctgtc gggcatgggc tgtactgtat gccgaatcgg taatccattc tggtttgccg
     8761 atggctttac aggtgttttc ccagttttgc tcctgaatag tgaaataaat ataggcgtta
     8821 ggatcggttt cccagccttt acatttcagg atccagccag gctgaccgcc accacctgca
     8881 ttaccaccgc ggggaactgc atcaccaaat gtaccattcg gatactgcgg gtattcttcc
     8941 agataaccca atttatcgag acgctgctgg tcacgtaatt tcacgcggca aaggttcaat
     9001 acggcatcct gcattgacat ggtgactcgt tgcccacgcc ccgttttttc gcgatgcagc
     9061 aaagcagcaa gtaaaccgat cagcaaatgc attccggtgt tgctgtcacc caacgctgca
     9121 gcgcttacca gcggcggacc atcccaaaaa cccgtagtgg atgccgcgcc acccgctgcc
     9181 tgagcaacgt tttcataggc ttttacattc acataaggcg aacactcatc aaaccctttg
     9241 atcgaaccaa aaatcagacg tggattgatt tcttgaatat gctcccaggt gaagcccatg
     9301 tgatcaatgg cccctggatg aaagttctcg actaagatat cagcttcgcg gatcagcttt
     9361 tccattacct ctttgccttc cgctgttttg gtatttaact caatagaacg tttgttactg
     9421 ttaagcatgg tgaagtaaag cgcatcgata tcaggaatat ctcgcagttg gtgacgcgtt
     9481 acgtcaccaa cgccgggacg ttcaatttta ataacgtcag cgccaaacca ggccagcatt
     9541 tgagtacaag atgggccaga ttgcacaccg gtgaaatcga gaactttaat tccttgaagt
     9601 ggagttgaca tagcaatacc tattagttaa ttaaatatgt gttaatttag cgtttattta
     9661 aggcataacg ccctctataa ataatctgca aaatatcttc gtagcggcac tcgatatttt
     9721 taaattcctg tatcaagtac tgaaaattat ttcatcgcca cttccaattc ctttctgcat
     9781 ggtaagtagt gaggacgaaa agaagaatat gataaatatt tattcgtggt aatcagagag
     9841 agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta
     9901 agagtatgat tacgcttttt aaagcgtacc ataaagcata acataataaa tgatttgtga
     9961 tgacactgga ttattgcggt ttactttgag gaaaataaaa tgcgtgttta tagaaccgcg
    10021 agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct
    10081 ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg
    10141 cttcgctatc gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt
    10201 attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg
    10261 gcatcagata ttggttttcc attacgccaa caccggccag gcgaagctct tccattgcgc
    10321 cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg
    10381 gtgttgctac ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat
    10441 cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg
    10501 acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttccggatcg ccatgaaaca
    10561 gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct
    10621 ctgctgctgc cgttttctgg gtttgctgag tttgctgttg tgccgctacc ggtgcattat
    10681 cagcagccca tacagttgaa gatgccagaa ttgctgtgac catcgtagcc ataattaaac
    10741 gtttcatact tacctcattc tttaacagtt ttagaaaacg ccgccatcat attgggtgag
    10801 catgaaatga gaaatcagag agcaacggtc attttgaaag ggttatctta caattgtagt
    10861 tattatcgca aagcggcatt tagtcttaca gttgtaggaa tatgcttcct gtcgcagaga
    10921 actattctta tataccacaa agagatgtca tttattatat tgatgtaaat attgattaat
    10981 tatatgtaaa tacagccata acatcgtatg gttctcagtg aaccattttc aggataactt
    11041 tctatgaaag taaacttaat acttttcagc ttatttttat tggtctctat tatggcatgc
    11101 aatgtttttg cattttccat ttctggtggt gtaagtgaga ggagctataa agagactgaa
    11161 aaaacatcag cgatgacaac cacacactct acaaaacttc agccatcaca ggcgattttg
    11221 tttaagatga gagaagatgc gccaccatta aacctcacag aagaaatcac gcctacttat
    11281 ccgacaaagg cgaattatct tattcatcct gtgcgatagc gtctgtcaca tggcaggtga
    11341 acaatcatgt ctgatgctga tgtgaacacc tgcccaaatg agccatcctg acccactatc
    11401 gcctgccata ccccaccata aagcgaatca tcaagccaca agccggggca agaaacataa
    11461 atatccccgt caccagttca ttaccgccag ttaaccacat ggtgaagtaa taaatgagtg
    11521 cagcgaggat caccatgccg aagaaagaag agaccgccga taccacgttc agtacctgac
    11581 cgatgtagcc gcttacgcag acaatacaca gggcgagaaa catccataaa taaatcatag
    11641 ctcaacctcc tgccgcgcta aaaaacctgt aacttcagtc actttaatag taagacaatc
    11701 cttaacctct ggcaattttt gctaagcatc aatgaaaaca gcacgttaaa tgcaacgcta
    11761 ttgcgtgcgg aaaatttttt actttgcacg attaataatc agtagctgaa agcagtcagc
    11821 cagtgataaa gtagagccac cgcattcatg ctcctcgtgg ttatgtcctg actagtcttt
    11881 acactcttta caggaaccat tgtcgtacat gatggcccaa ccaattgagt gtaccgctgt
    11941 cgacatggaa tcttctgcga tacaacaatt cgtatctaca gaaggtaact atgtttccac
    12001 aatgcaaatt ttcccgcgag tttctacatc ctcgctactg gctcacatgg tttgggcttg
    12061 gtgtactctg gctttgggta cagcttcctt atcctgttct ctgctttctc ggcacgcgta
    12121 ttggcgcaat ggcgcgacca ttcctgaaac gtcgtgaatc tatcgcccgt aaaaacctgg
    12181 aactttgttt cccgcagcat tctgcggaag aacgcgagaa gatgattgcc gaaaactttc
    12241 gttcactcgg catggcgctg gtagaaaccg gcatggcatg gttctggccc gacagtcgcg
    12301 tacgtaaatg gtttgatgtt gaagggttgg ataaccctaa acgcgcacaa atgcaaaatc
    12361 gcggcgtaat ggttgtcggc gtccatttta tgtcgctgga actgggcggc cgcgtgatgg
    12421 gactgtgcca accaatgatg gctacctatc gtccacataa taatcagctg atggaatggg
    12481 tgcagacccg tgggcgcatg cgctctaaca aagcgatgat cggcagaaat aatctgcgcg
    12541 gcattgtcgg tgcactgaag aaaggtgaag cggtatggtt tgctcccgat caggattatg
    12601 gtcgtaaagg cagctccttc gcgccgttct ttgcggtgga aaatgtcgcc acaaccaatg
    12661 gcacctatgt tctctcccgt ctctctggcg cagccatgtt gaccgtaacg atggtaagaa
    12721 aagcggatta cagcggatat cgtttgttta tcaccccaga gatggaaggc tacccgacag
    12781 atgaaaatca agccgctgcc tatatgaaca agattatcga gaaagagatc atgcgcgcac
    12841 cggagcagta cctctggatc caccgtcgct ttaaaacgcg cccggtggga gaatcgtcgt
    12901 tgtacattta acaaactgct cttactcttt ccctcctcaa atcggccata ataacctcat
    12961 ggtttttatg gcttttttct taaggttttt tccttcgctg aatatctaat tattaccgtc
    13021 agaaattaaa ctgtcgccga tatgagacac ttgtaagtta ctaaaagtaa ataagaaaat
    13081 gcctcttgtc tcgtttcagt tttaggcgta cattaacgcc gtcttgagca gtttagctca
    13141 gaattctggg gtgatgccca cggtaaaata cagggctttt aaggtcgctt tgcccggtgt
    13201 caactcactg tatcaggtgt aatgaagtca ttcaggcgta acagtaatta cgcggagaga
    13261 tgtaaagtga aatatttctt tatgggcatt tcttttatgg tcatcgtttg ggccggtact
    13321 tttgccctga tgatctaaaa gcaaactgtg caatgaaaaa caggagccat caggctcctg
    13381 tttgcatttc tgacgacggt gtttattccg cgttttcgtg aatatgtttg ctgctggcgg
    13441 gtagtaaacc gtcggcacgg aacatcgctt taatccctct gatggcctga cgaatacggt
    13501 cgcggttttc aatcagtgca aagcgaacat gcgtatcacc atagtcgcca aagccaatcc
    13561 ccggcgagac acaaaccttc gcttcgttaa ggagcttctt ggcaaattcc agcgatccca
    13621 tggccgcata tggttccggg attttcgccc agacatacat cgaagccttc ggcatttcga
    13681 ccatccagcc cgcttcatgc agccctttaa ccaatacatc acggcggcgt ttgtactgtt
    13741 cggtaatgtc gcgcacgcac tgttgatcgc cctcgagtgc cgcaatcgcc gccacctgca
    13801 acggcgtaaa agtgccgtaa tcgtgatagc ttttgattcg cgccagggca ctgaccagcg
    13861 ttttgttgcc gaccataaag ccgatacgcc agcccgccat gttgtagctt ttcgacagcg
    13921 taaagaactc gaccgccaca tcgcgtgcac ccggcacctg cataattgac ggcgctttcc
    13981 agccatcgta gacgatatcg gcataggcca ggtcatggac caccagcaca tcgtagcgtt
    14041 tcgccagcgc cacaaccttt tcgaagaact ccagttccac gcattgcgcg gttgggttcg
    14101 acgggaagcc gaggatcatc atcttcggtt tcggataact ttcacgaatg gcgcgctcca
    14161 gttcgttgaa gaaatcgaca ccttccacca gcggcactga gcgtacctgc gccccggcaa
    14221 tcaccgcgcc ataaatatgg atcgggtaac ttggattcgg caccagcacc gtgtcaccat
    14281 gatccagcgt cgccagcatc agatgcgcca ggccctcttt cgaaccaata gtgacgatgg
    14341 cttctgattc cgggtcgatt tcaacgtcgt agcgatcctg ataccagcgg gaaatggcgc
    14401 gacgtaaccg cggaatgccg cgtgaagtgg agtaaccatg cgtgtccggg cgctgggcca
    14461 cagtacataa tttttcgacg atatgcggcg gagtcgcacc gtccgggtta cccatgctga
    14521 aatcgataat atcttcgccg cgccgacgcg cagccatttt cagttcagcg gtgatgttaa
    14581 aaacgtaggg cgggagacga tcaatgcgcg taaagcgacg ttcagggcga gtgtcagcca
    14641 taatttcctc agattaacgt tagcgcccgg accgtccgag cgacgctgcc acaatggtgg
    14701 ctgttttgaa aatagcctga ttaatttctg tctgtccagc gttttagaac atttttatcc
    14761 atggctttaa atgaggaact ccacttcaaa a
//
LOCUS       DAZSBF010000096        14461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZSBF010000096 DAZSBF010000000
VERSION     DAZSBF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.097"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..160
                     /locus_tag="ACHXWD_002306"
     CDS             <1..160
                     /locus_tag="ACHXWD_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR7621210.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(198..1259)
                     /locus_tag="ACHXWD_002307"
     CDS             complement(198..1259)
                     /locus_tag="ACHXWD_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR7621211.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1472..2935
                     /gene="bepA"
                     /locus_tag="ACHXWD_002308"
     CDS             1472..2935
                     /gene="bepA"
                     /locus_tag="ACHXWD_002308"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR7621212.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2956..3315
                     /gene="arsC"
                     /locus_tag="ACHXWD_002309"
     CDS             2956..3315
                     /gene="arsC"
                     /locus_tag="ACHXWD_002309"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR7621213.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3453..4154)
                     /locus_tag="ACHXWD_002310"
     CDS             complement(3453..4154)
                     /locus_tag="ACHXWD_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR7621214.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="ACHXWD_002311"
     CDS             complement(4249..5538)
                     /gene="uraA"
                     /locus_tag="ACHXWD_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR7621215.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5624..6250)
                     /gene="upp"
                     /locus_tag="ACHXWD_002312"
     CDS             complement(5624..6250)
                     /gene="upp"
                     /locus_tag="ACHXWD_002312"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR7621216.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6575..7612
                     /gene="purM"
                     /locus_tag="ACHXWD_002313"
     CDS             6575..7612
                     /gene="purM"
                     /locus_tag="ACHXWD_002313"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR7621217.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7612..8250
                     /gene="purN"
                     /locus_tag="ACHXWD_002314"
     CDS             7612..8250
                     /gene="purN"
                     /locus_tag="ACHXWD_002314"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR7621218.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8421..10487
                     /gene="ppk1"
                     /locus_tag="ACHXWD_002315"
     CDS             8421..10487
                     /gene="ppk1"
                     /locus_tag="ACHXWD_002315"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR7621219.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10492..12033
                     /gene="ppx"
                     /locus_tag="ACHXWD_002316"
     CDS             10492..12033
                     /gene="ppx"
                     /locus_tag="ACHXWD_002316"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR7621220.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="ACHXWD_002317"
     CDS             complement(12072..14315)
                     /gene="pdeF"
                     /locus_tag="ACHXWD_002317"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR7621221.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         3820 a         3696 c         3606 g         3339 t
ORIGIN      
        1 ctggcaactg gcgcacagta gcgcagacaa ttttccggca ctgacggtaa gccattttat
       61 taccgccaat ctgctcccgg tgatgctggg taatattatc ggcggtgcag tgctggtgag
      121 tatgtgttat cgggctattt atttacgtca ggaatcctga aaaatcagcc cggcgaaaca
      181 gttcgtcggg ctgaagatta ttcttgcgct atttgcccat cgggccaggc gtggatcacg
      241 gctttgatca gcgtcgccaa tggaatagcg aagaatacgc cccagaatcc ccacaaacca
      301 ccgaagatca ccaccgataa aataatcacc agcggatgca ggttaaccgc ttcggagaac
      361 aacaccggta ctaacagatt accgtccagc gcctgaataa tcagatacac tgcgaagcag
      421 ctccagaatt ccgtgcctgc accaaactgg aatagcgcca cgccaaccac cggaatggtc
      481 accacaaatg cgccaatgta cggaataagt accgagaagc cgaccagcac cgccagcagc
      541 agcgaatagt tcagcccaaa gagcaagaac cccagccaag tggcgatccc caccacgatc
      601 atctccagca ctttgccgcg gatatagttg gtgatttgtt gattcatctc cttccacacc
      661 tgtcctgcca gtccacggtt gcgcggcagc acccgacgaa cggcgttcag catctgctct
      721 ttgtctttca gcaggaagaa gaccatcaat ggtaccagca ccagatagac ggcaatggtc
      781 agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg aatcgcccat ggtcagcatc
      841 cgactgcgca tattttcggc cattgcatca ataatgcccg cgtccattaa tgccggatag
      901 cggcgcggca acgtggcggc aaagtcagaa agcttattga gcatccccgg catatcgcgg
      961 atcaagtaga tgccctgttg ccaggcgata ggcagtacca cgaacgccat cagtagcaat
     1021 ataccgacga aaaccaccaa tacaatcgac gtcgcccagc ggcgggagca gccaatagat
     1081 tgcaggcgca cggttggcca ttccagcaaa taggccagca caatagccac cagcaacgga
     1141 gcaagcaggc cactaaagaa aaagataatg ccaaatccgg caactaaaat aaccagcaag
     1201 gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt gcatcaacat ttcgagcata
     1261 aagaccttcc ctgaacctca agagcgggat tgcgatccgc aattgtatcg aaatgtcaca
     1321 aaaaagactt cgctttttat gacggattca ggaaactgaa aagtcatttg agtgggctaa
     1381 tcttcgccgt tacactcaaa ggcggcgcgg tgggaacaat atttcacagt atcggtcaaa
     1441 tgactatctt cagaaataca ggatagaggt tatgttcagg cagttgaaaa aaaacctggt
     1501 tgcaaccctc attgctgcta tgaccattgg tcaggtagcc ccggcgtttg ccgacagcgc
     1561 agacaccttg ccggatatgg gaacctccgc aggaagcacg ctttccattg gtcaggaaat
     1621 gcagatgggc gactattatg tccgccagct acgcggcagc gcgccgttaa ttaatgaccc
     1681 gctgttaacg caatatatta attcgctggg gatgcgtctg gtttcgcatg ccaattcggt
     1741 taagacaccg tttcattttt ttctgatcaa caacgacgaa attaacgcct ttgctttctt
     1801 tggcggcaac gtggtgctgc actctgccct gttccgttat tccgataacg aaagtcaact
     1861 ggcttcagtt atggcgcacg aaatctccca cgtcacccaa cgtcacctgg cgcgagcgat
     1921 ggaagatcag cagcgcagcg cgccgctgac ctgggtcggc gcgttaggtt ctattttact
     1981 ggcgatggcc agtccgcagg cggggatggc ggcgctgacc ggtacactgg cgggaacgcg
     2041 tcaggggatg atcagtttca cccagcaaaa tgaacaggaa gcggaccgca ttggtattca
     2101 ggtgctgcaa cgctcgggat tcgatccgca ggcgatgcca accttcctcg aaaaattact
     2161 cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta ttgactcacc cgttgccgga
     2221 aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg cgcccgatgg tggtgcagtc
     2281 gtcggaagat ttctatctgg cgaaagcgcg cacactgggg atgtataatt ccggacgtaa
     2341 ccagctcacc agtgatttgc tggatgaatg ggcgaaagga aacgttcgtc agcaacgagc
     2401 ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat aaatacgacg aggcgcgtaa
     2461 aacgctgcaa ccgttactgg cggcagaacc tggcaacgca tggtatctcg atctggctac
     2521 cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc aatcgcctga aaaatgcccg
     2581 tgatttgcgc accaatccgg tgttgcagct caacctggca aacgcttatc tgcaaggcgg
     2641 tcaaccacaa gaagcggcca atattctgaa tcgctacacc tttaataata aagatgacag
     2701 caacggctgg gatttactgg cacaggcgga agccgcgcta aataaccgcg atcaggagct
     2761 ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc gatcaggcca tttcgctgtt
     2821 gagtagcgcc agttcgcagg tgaaattagg cagccagcaa caagcgcgtt acgatgcgcg
     2881 catcgaccag ttgcgccagc tgcaggaacg ctttaagcct tataccaaga tgtaattcgc
     2941 attaaaggaa gaacaatgac caaacaggta aaaatctacc ataacccacg ctgttcaaag
     3001 agccgggaaa cgctgaacct gctgaaaaaa aacggcgtgg agccagaagt ggtactctac
     3061 cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc tgaaaatgct ggggatgaat
     3121 agcgcccgtg aattgatgcg ccagaaagag gatctttata aagaactgaa cctggctgac
     3181 agttcgctta gcgaagaggc gctgattcag gcaatggtgg ataatccgaa gctgatggag
     3241 cgcccgattg tggtcgcgaa tggcaaagcg cggattggtc gtccaccgga gcaggtactg
     3301 gaaatcgtgg gttaagatgt gccccatcgc tagttgaagc acattgccgg atgcgacgct
     3361 tgccatgtct tatccggcct acgaatacta tggattaact tcgcgtagtt cggataaggc
     3421 gttcgcgccg catccgacaa taaacacctt atctacaact tcagaatttc tttcacaaac
     3481 ggaatggtca gcttacgttg cgcggtaatc gacgcacgat ccaactgatc caacgtcata
     3541 aatagcgtgc gcatttctct gtcgagccgc ttcagcaaga aacgccccac atcttccggc
     3601 agttcaaaac cacgcaaacg cgcgcgtaac tgtagcgcct gcaacttatc ttcatcagaa
     3661 agtggctgca atttgtagat ctgcccccag tcgagtcgcg acgcgagatc cggtaatccc
     3721 agattcaact gccgcggtgg acgatcgccg gtgatcaaca atcgtgtttt gcccgattcc
     3781 agaattcgat tgtagagatc gaaaatcgcc atctcccaca actcatcgcc tgcaatacac
     3841 tcaatgttgt cgatacagac cagcgacaaa tgctccatac cgtcgagcac ttccggaaca
     3901 aaccaggtgc gtttatccag cgggacatag cccaccgcat cgccacgctg cgacaattcc
     3961 gcgcaagccg cgtgcagcag atggctgcgc cccgcgcctt cgcgtgccca gagatagatg
     4021 taaccgctat gttcctgacg cagcacgttt tgcagcgcag ccagtaaaga ggagttatcc
     4081 cccggccaga aacttgcaaa ggtttcgtcg tcaggaagat aaagtggcaa agagagctgt
     4141 gccggtgtgt tcagagatac ctcaaccagg atttcacaaa atcgcgagaa gtttaccaca
     4201 gaatcccatg atgtttgaac cgggcagcaa cactgcccgg tcggtacatt atttgtctgt
     4261 tatatccgcg tcttctgcgt ccagcaccac ttcttcaggg cgcagcacac taatcagttt
     4321 gaagatcaaa cttaagccga taccgacgat ggtcgccagc gccatacctt tcagctccgc
     4381 cgcgccaatg tttaccttcg cgccactgac accgatgatc aaaatcacgg aagtcaggat
     4441 caggttctgt gctttgttgt aatccacttt tgattcgatc aaaacacgaa taccggaagc
     4501 accgatgaca ccataaagca gcagcgaaac gccgcccatc accggcaatg ggatcatctg
     4561 gatagcggca gccagtttac cgacgcagga aagcaggata gcgaaaatcg ccgccccacc
     4621 gataacccag gtactgtaaa cacgggtgat cgccatcacg ccaatgtttt ctccgtaagt
     4681 ggtatttggc gtagagccaa agaagccgga gatcacagtc gacaggccgt tagcaaacat
     4741 cgaacggtgc agacctggat cgcgcagcag atcttttttg acgatattag ccgttactac
     4801 caggtgccct acgtgttcgg caataaccac taacgccgct ggcagaatag tcagaatggc
     4861 aaaccactcg aagcgcggcg tatagagggt tggcagcgca aaccagtgag cattaataat
     4921 cggcgtggta tcgacaattc ccattgcgaa agagagcgcg taccccacca gcacgccaat
     4981 taaaatcggg ataattgcca ggaaaccacg gaacagcacg gaacctaaaa ccgtgaccgc
     5041 cagggtggta atagagatga tgatggtttt ggagtctggc gtttgccctt cagccgggag
     5101 taaacccgcc ataccggcag ctacgcccgc cagctccaga ccgatgacgg caacgattgc
     5161 gcccattgcc gcaggtggaa acagcacgtc cagccagccg gtccccgctt tcttcacgat
     5221 aaaagaaacc aggcagaaca gcacgccgca cataataaag ccgcccagcg cgacttcata
     5281 ccctaacggc aacagtaaca ataccggtga aataaaggca aagctggaac caagataagc
     5341 cggaattttc cctttacaga tgaagagata cagcagcgtt ccaataccgt taaataacag
     5401 tacagtcgcc gggttaatat gaaataagac gggcaccagg acggttgcac caaacatggc
     5461 gaacaaatgt tgcaaactaa gcgggattgt ctgtaaaagt ggcggtcttt cactcacccc
     5521 gatagcacgg cgcgtcatag tattatcctc tgtattatgt gttataggcg ctttactcaa
     5581 aaaaaagccg actcttaaag tcggctttaa ttatttttat tctttatttc gtaccaaaga
     5641 ttttgtcacc ggcatcgccg aggcccggaa taatgtatcc gtgctcgttc agtccctggt
     5701 caatcgatgc ggtatacagt tcgacgtccg ggtgcgcttt ttccagcgca gcgatacctt
     5761 ctggcgcagc taccagcacc agaactttga tgctgctgca gcccgctttt ttcagcaggt
     5821 cgatggtcgc gataacggaa ccaccggttg ccagcattgg gtcaacgatc agcgccatac
     5881 gctcatcgat gttagaaacc agtttctgga agtacggtac cggctccagc gtttcttcat
     5941 tacggtacat accgacaacg ctgatgcgcg cgctcggaac attttccagc acaccgtcca
     6001 tcatccccag accggcacgc agaattggca caacggtaat tttcttacct ttgatctggt
     6061 cgatttctac cgggccgttc cagccttcga tagttacttt ttccgtttcg aggtcggcgg
     6121 tcgcttcgta agtcagcagg ctacccactt cggaagcgag ttcgcgaaag cgcttggtgc
     6181 tgatatcttg ctcacgcatc agtcccagct tgtgtttgac gagtgggtgt ttgacttcca
     6241 cgatcttcat actctttctc ctttgagggg cagccacaaa aaaaatcgac ggattatacc
     6301 tcctttcttc aaggcggcaa tattcttttc gttgacttta gtcaaaatga taacggtttg
     6361 agataaagtt attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa
     6421 cctttgccct ggcacaagtc ttctttcgcc gcgcgtctgg ggaaaagacg tgcaaaaagg
     6481 ttgtgcaaag cagtctcgaa aacgtttgct ttccctgtta gaattgcgcc gaattttatt
     6541 tttctaccgc aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta
     6601 caaagatgcc ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt
     6661 agtgaagaaa acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg
     6721 tgcattgccg caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac
     6781 caagctgcgt ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc
     6841 catgtgcgtt aatgacctgg tggtgcaagg tgcagaaccg ctgtttttcc tcgactatta
     6901 cgcaaccgga aaactggatg ttgataccgc ttcagcggtg atcagcggta tcgcggaagg
     6961 atgtctgcaa tcgggctgtt cactggtggg tggcgaaacg gcagaaatgc cggggatgta
     7021 tcacggtgag gattacgatg tcgcgggttt ctgcgttggc gtggtagaaa aatcagaaat
     7081 catcgacggc tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc
     7141 gcactcgaac ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca
     7201 aaccaccgaa cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta
     7261 cgtgaagtca gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac
     7321 cggcggcggc ttctgggaaa acattccgcg cgtattgcca gataatactc aggcagtgat
     7381 tgatgaatct tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg ctggtaacgt
     7441 tgagcgccat gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc
     7501 tgctccggaa gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa
     7561 aatcggtatc atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt
     7621 gtggtgctta tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc
     7681 aacaaaatta aaggcaccat acgggcagtt ttcagcaata aggccgacgc gttcggcctt
     7741 gaacgcgccc gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt
     7801 cgtgaagcct atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc
     7861 gtgctggctg gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt
     7921 ttgctgaaca ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag
     7981 gcgctggaaa atggtgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg
     8041 gacggtggcc cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat
     8101 gacatcaccg cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg
     8161 tttgccgatg gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg
     8221 ccgccgcagg gctacgctgc cgacgagtaa tgccccgtag ttaaagcgcc agctctgccg
     8281 ctggcgtttt tcaattcacc tgtaaatcgc aagctccagc agtttttttc ccccttttct
     8341 ggcatagttg gacatctgcc aatattgctc gccataatat ccaggcagtg tcccgtgaat
     8401 aaaacggagt aaaagtggta atgggtcagg aaaagctata catcgaaaaa gagctcagtt
     8461 ggttatcgtt caatgaacgc gtgcttcagg aagcggcgga caaatctaac ccgctgattg
     8521 aaaggatgcg tttcctgggg atctattcca ataaccttga tgagttctat aaagtccgct
     8581 tcgctgaact gaagcgacgc atcattatta gcgaagaaca aggctccaac tctcattccc
     8641 gccatttact gggcaaaatt cagtcccggg tgctgaaagc cgatcaggaa ttcgacggcc
     8701 tctacaacga gctgctgctg gagatggcgc gtaaccagat cttcctgatt aatgaacgcc
     8761 agctctccgt caatcaacaa aactggctgc gtcattattt taagcagtat ctgcgtcagc
     8821 acattacgcc gattttaatc aatcctgaca ctgacttagt gcagttcctg aaagatgatt
     8881 acacctatct ggcggtggaa attatccgtg gcgataccat ccgttacgcg ctgctggaga
     8941 tcccatcaga taaagtgccg cgctttgtga atttaccgcc agaagcaccg cgtcgacgca
     9001 agccgatgat tcttctggat aacattctgc gttactgcct tgatgatatt ttcaaaggct
     9061 tctttgatta tgacgcgctg aatgcctatt caatgaagat gacccgcgat gccgaatacg
     9121 atttagtgca tgagatggaa gccagcctga tggagttgat gtcttccagt ctcaagcagc
     9181 gtttaactgc tgagccggtg cgttttgttt atcagcgcga tatgcccaat gcgctggttg
     9241 aagtgttacg cgaaaaactg actatttccc gctacgactc catcgtcccc ggcggtcgtt
     9301 atcataattt taaagacttt attaatttcc ccaatgtcgg caaagccaat ctggtgaaca
     9361 aaccactgcc gcgtttacgc catatttggt ttgataaagc ccagttccgc aatggttttg
     9421 atgccattcg cgaacgcgat gtgttgctct attatcctta tcacaccttt gagcatgtgc
     9481 tggaactgct gcgtcaggct tcgttcgacc cgagcgtact ggcgattaaa attaacattt
     9541 accgtgtggc aaaagattca cgtatcatcg actcgatgat ccacgctgcg cacaacggca
     9601 agaaagtgac cgtggtggtt gagttacagg cgcgtttcga cgaagaagcc aacattcact
     9661 gggcgaagcg cctgaccgaa gcaggcgtgc acgttatctt ctctgcgccg gggctgaaaa
     9721 ttcacgccaa actgttcctg atttcacgta aagaaaacgg tgaagtggtc cgttacgcac
     9781 acatcgggac cgggaacttt aacgaaaaaa ccgcgcgtct ttatactgac tattcgttgc
     9841 tgaccgcaga tgcgcgtatc accaacgaag tacggcgggt atttaacttt attgaaaacc
     9901 cataccgccc ggtgacattt gattatttaa tggtgtcacc gcaaaactct cgccgtctgt
     9961 tatatgaaat ggtagaccgc gaaatcgcca acgcgcagca agggctgccc agtggtatca
    10021 ccctgaagct aaataacctt gtcgataaag gcctggttga tcgtctgtat gtggcctcca
    10081 gctccggcgt accggttaat ctgctggttc gcggaatgtg ttcgctgatc cccaatctgg
    10141 aaggcattag cgacaacatt cgtgccatca gtattgttga ccgttacctt gaacatgacc
    10201 gggtttatat ttttgaaaat ggcggcgata aaaaggtcta cctttcttcc gccgactgga
    10261 tgacgcgcaa tattgattat cgtattgaag tggcgacacc gctgctcgat ccgcgcctga
    10321 agcagcgggt gctggacatc atcgacatat tgttcagcga tacggtcaaa gctcgttata
    10381 tcgataaaga actcagtaat cgctacgttc cccgcggcaa tcgccgcaaa gtacgggcgc
    10441 agttggcgat ttatgactac atcaaatcac tcgaacaacc tgaataaccc tatgccaata
    10501 cacgataaat cccctcgtcc gcaggagttt gctgcggtcg atcttggttc aaacagtttt
    10561 cacatggtca tagcccgtgt ggtagatggt gccatgcaga ttattggccg cctgaaacag
    10621 cgggtgcatc tggcggacgg cctggggcca gataatatgt tgagtgaaga ggcaatgacg
    10681 cgcggtttga actgtctgtc gctgtttgcc gaacggctac aagggttttc tcctgccagc
    10741 gtctgtatag tcggcaccca tacgctgcgt caggcgctga acgccactga ctttctgaaa
    10801 cgcgcggaaa aggtcattcc ctacccgatt gaaattattt ccggtaatga agaagcccgt
    10861 ctgattttta tgggcgtgga acatacccaa ccggagaaag gtcgcaaact ggttattgat
    10921 attggcggcg gatctacgga actggtgatt ggtgaaaatt tcgaaccgat tctcgttgaa
    10981 agccgccgga tgggttgtgt cagctttgcc cagctttact tccctggcgg ggtcatcaat
    11041 aaagagaatt ttcagcgcgc tcgtatggcg gcagcacaaa aactggaaac tttaacctgg
    11101 caattccgta ttcagggctg gaacgtggca atgggcgctt ccggtaccat aaaagccgcc
    11161 catgaagtgt taatggaaat gggcgagaaa gacgggataa ttaccccgga acgtctggaa
    11221 aaactggtaa aagaagtttt acgtcaccgt aatttcgcat cgctgagttt accgggtctt
    11281 tccgaagagc ggaaaacagt cttcgttcct ggactggcga ttttatgcgg tgtgtttgat
    11341 gctttagcca tccgtgaact gcgcctttct gacggggcgt tacgcgaagg cgtactgtac
    11401 gaaatggaag gacgtttccg tcatcaggat gtgcgtagtc gcaccgccag cagcctcgcc
    11461 aaccagtatc acatcgacag cgaacaggcc cgacgggtgc tggataccac tatgcaaatg
    11521 tacgaacagt ggcgggaaca gcaaccgaag ctggcgcatc cgcaactgga ggcgctactg
    11581 cgatgggccg ccatgctgca tgaggtcggg ttgaatatca accacagcgg tttgcatcgc
    11641 cactccgctt atattctgca aaacagtgac ttgccgggtt ttaatcagga acagcagctg
    11701 atgatggcga cactggtgcg ctatcaccgt aaagcgatta aactcgacga tctgccgcgc
    11761 tttaccttgt ttaagaagaa acagttcctg ccactgattc agctattgcg ccttggcgta
    11821 ttactcaaca atcaacgtca ggcaaccacc acaccgccaa cattgacact gattactgat
    11881 gacagtcact ggacactgcg tttcccgcat gactggttta gtcagaatgc gttggtactg
    11941 cttgatctgg aaaaggagca agaatactgg gaaggcgtgg ctggctggcg gttgaaaatt
    12001 gaagaagaaa gtacacctga aatcgccgct taatgctttg cgggccgacg agaaatgtcg
    12061 gcccgcatta ttcaggcact ttcgcgaatg ggttcgattt cattcaacgt atcaattaac
    12121 ggctgcggct taccaataag ataaccctgc atataatcga tccccaaaga gagcaccgcc
    12181 tcgcggatct cttcgttttc aacgtactct gccactaccc gcattttctt cattcgcgcc
    12241 agatggcaaa tcgacgccac aatctgataa tccagactat ttgacacaat attgcggata
    12301 aagctgccgt caattttaag cagatcggca ttcacatttt taagccgtgc atagctggcg
    12361 tagccggtgc cgaaatcatc aatcgcaatc tggcagccta attcctgaag atgctgcaag
    12421 gtgatttgcg cctgcttaac attggtcaga gcattacttt cggtgacttc aaaaataagt
    12481 tgccacgctt caatctgata tttagccagc aactgactga cttcaacagg aaaacgagcc
    12541 tgacataccg aggttggaga cagattaata gcaaaacggt gagcgggcat cttcgctctg
    12601 ttttcagcca taaattgcag cgtatgctca atgacccaca tgtcgatact cgacgataaa
    12661 ccaaattcgt gcgcgaccgg caagaagcta tcggggacga tcagttcatc attctcacct
    12721 ttcatgcgca gaagaatttc atggtaaaca tcaccacgca taccggtaat cggctgggcc
    12781 atcaggaaaa aatggttgtg ttccagcgcc tgctgtagtc gattcatcat cgcgacttta
    12841 tctttcaatt cgcgttgcaa atacattgcc ccgcgacgct gcatattttc cggggcgttg
    12901 gtcacgatgg aaagttcggc gaccgtattt agctctccca gcagcaggta gatatgattc
    12961 actggcgagc gcacatagca gtaactgacg ccaatctgcg gttgcatcgg catcccatcc
    13021 caaaagaaac gaaattgctt gagatgacta tccagtgcgg taatgcgctc ctggtgcgat
    13081 tctgtattca gtcgcagcgc gagatcgtta cccgaaagct gataaacatc ttcacccggt
    13141 tccagcaagg gtgacagcca gtgagaaagt ttttgcttgt attgaatccg cagcatgatg
    13201 ccatagttct taaccagcat ttccatgcca gggatgcgta aataacaaag cgcagaccag
    13261 ggagcatcac gcaacgcgcg attcagggcg cgaacatttg gcagatgaac caccgggtcc
    13321 atatacgcaa gccgctgaat gcgtctgact accgctctct gacgggttgc cagtacagcc
    13381 atataattga caataaaaga gaataccaga taactggagg aggttatggt cagctgcgtg
    13441 gtatagccag gataaatggg aatgtaattt tgatagctgt ggatgctgat catcaacacg
    13501 accgcccaga gcagcgaaat cagcttataa ccatagcgca tcgctcccca catcatcagg
    13561 ggcagcaata atgacaaggt ataattggtg ctaaaaattg tgcttttttc atttaacggc
    13621 atgcataaca gcaacaataa agcacctaat gccagtagcc agagcgcgaa ctcttttttg
    13681 gtgactttgg catcaacctg ctgttttaat tgcgaataat agctacgtaa ataaaatgga
    13741 tttcgcacta cccgaatgat gaagtagcac agcgggacac cgatcagatt acccaccagt
    13801 aaggcctgat aattgattaa agtcccgagg ttaaaaggca tgacaccgac cagattttct
    13861 ctgctcgcca gtaatcctac aaatgcagca aactggaaaa gtatcagaaa cagcgttgca
    13921 ggaaacacaa tctgccagaa tatacgctgg gaaattaaac gggtatcacc atgtgaaacg
    13981 ttgttacgcc ggggagtaaa gaccctgtaa ccgccccaac agagaatgat aatgataata
    14041 aaattagccg ttaatgatag cgtttcgtaa aaacccagct ctgcatattt gcggacaaaa
    14101 atccctaagg cgatccctgg caatgccgcc cagctgaaaa acatcatcat gctgatcatc
    14161 agtgccagag gcagataaaa gagaaaaacc tcaccggaag aaatatgcgc gaaagtatta
    14221 atgtgggcga aaatgggcaa gattaaagaa ggcaggaaca gcggaagccc ccaccattta
    14281 tcacgcattt ttatataagt tgcattcagt ttcatagatg ctcagcagaa tcccccacat
    14341 cctgaaggag gtgtattcag acaggcatcc cacctgactt cgaatgatga ttattcatca
    14401 ctatagagag cattgattct aagtgtcata tgaaagttcc aattgatata tatcaaacaa
    14461 a
//
LOCUS       DAZSBF010000097        14421 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZSBF010000097 DAZSBF010000000
VERSION     DAZSBF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.098"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            41..397
                     /locus_tag="ACHXWD_002318"
     CDS             41..397
                     /locus_tag="ACHXWD_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR7621222.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(448..1344)
                     /gene="yhaJ"
                     /locus_tag="ACHXWD_002319"
                     /pseudo
     CDS             complement(448..1344)
                     /gene="yhaJ"
                     /locus_tag="ACHXWD_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1449..2150
                     /locus_tag="ACHXWD_002320"
     CDS             1449..2150
                     /locus_tag="ACHXWD_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR7621223.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2173..2337
                     /gene="yhaL"
                     /locus_tag="ACHXWD_002321"
     CDS             2173..2337
                     /gene="yhaL"
                     /locus_tag="ACHXWD_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR7621224.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2471..3781)
                     /gene="cyuA"
                     /locus_tag="ACHXWD_002322"
     CDS             complement(2471..3781)
                     /gene="cyuA"
                     /locus_tag="ACHXWD_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR7621225.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3809..5140)
                     /locus_tag="ACHXWD_002323"
     CDS             complement(3809..5140)
                     /locus_tag="ACHXWD_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7621226.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5415..6779)
                     /gene="tdcG"
                     /locus_tag="ACHXWD_002324"
     CDS             complement(5415..6779)
                     /gene="tdcG"
                     /locus_tag="ACHXWD_002324"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR7621227.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6851..7240)
                     /locus_tag="ACHXWD_002325"
     CDS             complement(6851..7240)
                     /locus_tag="ACHXWD_002325"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR7621228.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7254..9548)
                     /gene="tdcE"
                     /locus_tag="ACHXWD_002326"
     CDS             complement(7254..9548)
                     /gene="tdcE"
                     /locus_tag="ACHXWD_002326"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR7621229.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9582..10790)
                     /gene="tdcD"
                     /locus_tag="ACHXWD_002327"
     CDS             complement(9582..10790)
                     /gene="tdcD"
                     /locus_tag="ACHXWD_002327"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR7621230.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10816..12147)
                     /gene="tdcC"
                     /locus_tag="ACHXWD_002328"
     CDS             complement(10816..12147)
                     /gene="tdcC"
                     /locus_tag="ACHXWD_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR7621231.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12169..13158)
                     /gene="tdcB"
                     /locus_tag="ACHXWD_002329"
     CDS             complement(12169..13158)
                     /gene="tdcB"
                     /locus_tag="ACHXWD_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR7621232.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13257..14195)
                     /gene="tdcA"
                     /locus_tag="ACHXWD_002330"
     CDS             complement(13257..14195)
                     /gene="tdcA"
                     /locus_tag="ACHXWD_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR7621233.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3737 a         3858 c         3360 g         3466 t
ORIGIN      
        1 ggaatagact ttcgtcactt ttaattaaag ggatgttttt atgcagtggt atttatccgt
       61 attaaaaaat tatgttggtt tctctggtcg cgcacgtcgt aaagagtact ggatgtttac
      121 tctgattaac gccatcgtcg gcgctattat caatgtcatt caattgattt taggtctgga
      181 gcttccatat ctgtctatgc tttatttgct ggcaaccttc cttcccgtcc ttgcgcttgc
      241 tatccgtcgc ttacacgata ccgaccgttc aggtgcctgg gccttgctct tttttgttcc
      301 gtttattggc tggctcgtgc ttctggtttt tttctgcaca gaaggtactt ctggcagcaa
      361 tcgttacgga aacgatccga agtttggttc aaattaatct tagaattggg gcgatatttc
      421 gccccttttt attaacaata ataaatatta ttttccgcta aaaagtttgg gaatttcccg
      481 cagacaccag gatttcgctt cccccatact gtcacgacgc caggccataa taatatcgat
      541 ctcactggtc gattccgggc tgacgacacg caaccgccct tccgcaatat ctttttcgac
      601 catcggatac ggcatcgtcg ccacgccaag ccccgccagt aatgcctgac gtttatcttc
      661 aatcgtgctc accgttaagc gcggctgttt gtccagcagt tgtacggtca acaccgggcg
      721 ctcacgagcg gtatccgcta ccgcaattcc acgatatttc acgcgcgtca cttcagataa
      781 cggttccggc tcctgatgaa tcgggtgatc aggcgcggca acgtagacgt tcattaacgt
      841 atagagcttg cgcgagttga tctccgacga ggaacgaaaa tgcatatccg gcgcgataac
      901 aatatccgcc cgcccctgct ccagtcgttc ctacgccccc gccagcactt cggtgatgat
      961 tgccagttgg gtattggctt ttgccgccag tttgtcgatt aacgggaaaa aggcaggtgt
     1021 cggtaccagc gcttcggtca caatggtgag atgcgtttcc caaccgcgcg cgagggcttc
     1081 cgcatcggta gtcagtttat ctgcggcttc cagcaaaacg cgccctcgct ccagcaacat
     1141 cctcccgaca ttggtgaatt tggtgcgatg gcccgagcgg tcaaacagca ccacatccag
     1201 ctcttcttcc agtttttgca tggtgtagct aagtgcggaa ggcacgcgtc ccagctcatc
     1261 cgccgccgcc gcaaaactgc cccggcgatc gatcgcatcc ataacccgta gtgcttccag
     1321 cgttaatgcc ctttctttgg ccatttcgtt ctcattcagg aaatttgaac ataccgggca
     1381 gaatatctgg ctaacaatgt agcgtccagc cccttaccat aaaaggaagt aaagagaggt
     1441 caagaattat gattactacc cgaactgcca ggcagtgtgg acaagcagac tacggatggt
     1501 tgcaggcccg gtatactttt tcctttggac actacttcga cccgaaattg ttaggctatg
     1561 cctccctgcg tgtgcttaac caggaagtgc tggccccagg tgccgccttt cagccgcgaa
     1621 cctatcccaa agtcgatatt ttaaatgtga ttctggatgg agaagcagag tatcgcgaca
     1681 gcgaaggcaa tcatgttcag gccagcgccg gtgaggcgtt gctgctctct acccagccgg
     1741 gtgtcagcta cagcgaacac aatctcagca aagacaaacc gttaacacga atgcagcttt
     1801 ggctggacgc ctgcccgcag cgagagaatc cgctgattca aaaactggcg cttaatatgg
     1861 gcaagcagca attaatcgcc tcgccagagg gtacgatggg aagcctgcaa ttacgccagc
     1921 aagtgtggct gcaccatatc gtgctcgaca aaggcgaaag tgcgaatttc cagttgcatg
     1981 ggccacgcgc gtatttgcaa tcgattcacg ggaaatttca tgcgcttacg catcatgaag
     2041 agaaagcggc gctgacctgc ggtgatgggg cgtttattcg tgacgaggct aacattacgc
     2101 tggttgccga ttccccactg cgcgctttgc tgatagattt gcctgtctag ttgtttttac
     2161 agggagatga tgatgagtaa aaaatcggcc aaaaagcgcc agccagtgaa gcccgtggtg
     2221 gcgaaagaac ctgctcgcac cgccaaaaat tttggctatg aagagatgtt gagcgagctg
     2281 gaagccatcg tcgcggatgc tgaaacgcgt ttagccgagg atgaagctac cgcgtaaaga
     2341 caggttgccg gatgcggcga aaacgcctta tccggcctac aaaatccaac aacattaacg
     2401 aattaaacaa cccgtaggcc ggataaggtg cgccagcatc gcatccggca caatactcaa
     2461 aacctggcgt ttatctggcc ttgctcgcca taatctcgat aatctgccga tccgtttgct
     2521 gcatcgaatg gcttgctaac gcacacaggt tggcaatcga ctgctcaaca tcatgcgcca
     2581 cgatcccttc attgccggtc acggcggtat catccagcgc cattaacacc gctttccacg
     2641 cagccgaagc actggtcgaa accttcatcg cgcagctgtt cgacgcacca tcgcaaatca
     2701 tgccgctgac atcgccgatc atactgctga tcgccatcga gatagtttca taacgcccat
     2761 ccaccagcca tgccatcccg gcggcggccc ccattgctgc ggtcgttgcg gcacaaagtg
     2821 cagacaaacg cggtaactgg ttatggatgt aaattgcgct caaatgagaa agcatcagcg
     2881 cacgcgccag tcgttcatca tccgctccga agtgttctgc tacaaccacc acaggcattg
     2941 ttgcggtgat cccctggtta cccgagccgg agttactcat agccggaagc gtagcgccgc
     3001 ccatacgcgc atcggatgcc gcgctggtac gaatcacaat ggatgaagag agatctttcg
     3061 ccagcaaacc gcgctcgcac tgtttttcca gcgtcgcgcc aatatgcagc ccccacttac
     3121 cgctcaaacc ttcctgcgat aacgcacaat ttagcttcgc ggaatcgaga ataaagcgga
     3181 tcgccgcaaa cgggacttca ttgacgaact tcaggatctc agccagcgtc gttctggaaa
     3241 gcaccgtcag cggagactct tgctcgccct ctgccacaca cgcctgctgg gtaaacacca
     3301 caccattgtg cgtctcaata cgcacaatgt tggtatgccc gccgacgatg gtgacacacg
     3361 cccacttctc accgttccag actttggcgc gtgagaagag gatttcattg caaggttcct
     3421 ggatcttaac ggagactttc cccgccgcca gcagtgcttt ggcatcggca attgcctgcg
     3481 cagttgcgtc tttcagcact tccagcccgg cgttggcatt tccacctaac gcccccagcg
     3541 ccgccgcaat cggcagcccc accattcccg tgccgggaac ggtgacgccc agaccgttct
     3601 tcatcagatt tggcgaaacc caggcttcta cacgttcaac cggaccttcc agttctgccg
     3661 cagcaaccgc cgccgccagc gccagtgaaa tcggttcagt acatcccagc gccggtttta
     3721 cttcctcctg aacggcgagg atgtaacgct gccataacgg atttaaagtc gaatcaaaca
     3781 tattaaaaac cttaaaattt caggtaaatc aagaaaatgc caggaacgga gaaacacaaa
     3841 gcaacaaacc agtgacgata atcaggtaca gagacatccc tttgtatttg tgcaatgccg
     3901 gtactttgta aaccagccac gccgggatca ggcaccctac catgccgaaa atcgggctac
     3961 agatagaggt gaagctcaac accggtgcgt tcagtacgat agcgctccag gccagcaaaa
     4021 tggcgaaaat catgatgccg cgctgaacaa gattttcgtt aatcttctcg gcaggcatct
     4081 tgcgacgcag gatgttcatt acgatccctt gcgttgcttc gcgaaagcct aaatagacgc
     4141 caaagaatgc agtcatgaca gcaaagatat tgagaatgac gctgaccact ttcacccacg
     4201 ctgcgccgtc accgctaata aactgtgcgg caatcgccag cgcggaaata ttctgctcgt
     4261 aggcttttac cgcttcgtca tgccccatcg ccagcgtgaa cgacacggcg tagaaaaaga
     4321 cggtgacaaa caaaatgcca aacgcgatat tcattgcccg caatgcttta tgccgcgcca
     4381 cttcaataga tttttcccgc gagcgataag agatcaccat cgggcttaac gtctggataa
     4441 acaaaatcga cgtcagggta aacggcagcg taataatggc gtttttcacc agcagcccca
     4501 gcggcggtag cgaaccgacg ttatacagat gccacattcc caccatcgac acgcccagcg
     4561 ccgcgaccac cagcagcttg gtcagcacca tgccggtcga aattttgaat aacaatttct
     4621 cgccgcgtga agagatcgcc accagaatgc aaatcagtac cagaccataa aagggactgt
     4681 ctgacagcaa cccttccgtc acgccgaagg tatgcagata ggaagcacta tcgttggtga
     4741 tggcggtgga atagacgaac atccaaatca ccagcattac gaaatagagc gcacctaaca
     4801 ggatgcccca gtttttacct aaataaccgc taatgacact cgggtaatct ttacattctg
     4861 gtgattctgc cagcgtatta ataaacaacc gctgaaacag atacattgcc gggtaaccaa
     4921 tcaccgatga gagcaaaaat acccacaatc ccatcaaacc gacctgcacc gggagaaaaa
     4981 caatccccgc gccaatcgcc atcccgatac tcataatcac ccagccggtg tcagtactgt
     5041 cgaatttgat cgcttctcgc cactcgctct cactcattcc agcacgaccc gccggggtcg
     5101 aagcgtctgc aatgacgcct ttattcgatg cgatttccat aatttctcgc tcaatatttt
     5161 gtagggctta ttattttttt ccgagccgca tcaaggcgat atgcggtcta cgtttttgca
     5221 ggcgagttat tagaataaag aaatgatacg cgcgaggtca gagaaaatct tcacaatcaa
     5281 gactttgaaa taacggagat ggataagaat tttctactaa attaatcgca agagaatatt
     5341 cattctctat ttgcggcaga tcacaaaaaa aaggtgcaca tttgtgcacc caaggatgaa
     5401 agctgacagc aatgtcagcc gcagaccact ttaatggcca gtcctccgcg tgatgtttcg
     5461 cggtatttat cgttcatatc tttgccggtt tcatacatcg tctcgatcac tttatcgagt
     5521 gaaacacgcg gtgccgaggt gcggcgcatc gccatccacg cggcgtttac tgctttcacg
     5581 gcattaatgg cattacgttc aatgcacggg atttgtacct gtccggcaac cggatcgcag
     5641 gtcagcccaa ggttatgctc catcgcgatt tccgccgcat tgcatacctg cgccggactg
     5701 ccgcccagta gttcagttaa ccctgccgcc gccattgaac aggccacgcc aatctccccc
     5761 tgacagccga cctccgcgcc agagatggag gcgttcattt tatacagcgc gccaatagcc
     5821 cccgctgcca gaaaatagcg ggcaattgac cgctcgttta ccggacgacg gaacttatcg
     5881 taataagcca gcactgccgg aataatgccg cacgcaccgt tagtcggtgc cgttaccacg
     5941 cgcccgccag ctgcgttttc ttcactaacc gccagcgcgt acatgttgat ccagtcgatg
     6001 acattcatcg gatcgttaga gatgttatcg ctagaaacca gctgacgacg cagcgctacg
     6061 gcacgacgcg gcacattgag cggaccaggc agcacgcctt cagtgttcat cccgcgttca
     6121 ataccgtcat gcatcacttg ccagatacgg gcaaatcctg cgtcaatttc cgctttgctg
     6181 cgcagcgcca gctcgttgtg catcatcagg ccagatatag acaggccgtt gtaatcacac
     6241 attttcagca gttcacctgc tgagtggaaa tcgtaaggta cggacgtttc gacatcgtgc
     6301 gacaggccga agtgttcttc ttcgacaata aacccgccgc cgacagagta ataggtttta
     6361 cttaatagcg cttcctgtcc cttccaggca gtgatccgca ttccgttctc atggcgaggc
     6421 aacatttcgg gatggaagat aatgttcttt gctacaggaa aatcaacaat atgcgcacca
     6481 gatgccactg gcagccgccc gctgcgcgtt actaactcta taaatgcagg gatctcatca
     6541 atgacaacat cctgcggact gtttcctgcc agtcccatga taatggcgac atccgtggca
     6601 tggcctttgc ccgtcagtga caacgaccca tacagatcga ccacaatatg gctcgtcgcg
     6661 gttaataagc cgctactttc cagccgatca ataaaacttt ttccggcatt cattggcccc
     6721 acggtatgcg aactggaggg accaatccca attttgaaaa tatcgaatgc actaatcata
     6781 tccacaccct cggattgccg ttcagtgaag tggagcggaa cgaccttgcg accgtcccgc
     6841 tcacgaggct ttacgcacta cgtactgcga tagcttcaat ttccagcttc acatctttcg
     6901 gcaaacgcgc aacctgcaca cagctccggg tcgggtaggt cgcctgatgc tcatcgaaga
     6961 actgcttata gacttcattg atggtggcaa aatcattcag atcggtgatg aacacggtca
     7021 tcttgatgat atcgcccacg ctcagcccgg cagcaaccac gatcgctttg acgttttcga
     7081 ggcttaaaca cgcctgatct tgcacatcag ccgggatctc accggtctgc gggcaaaccg
     7141 gaatttgccc ggaggtgaag accatgctgc ctaaatcaac gccctgaaca taagggccga
     7201 ttgcgcctgg ggcacgttgc gtttcgataa tctttttcat acatcctccg gcgtcagagc
     7261 gcctgggtaa aagtacgtga aataacatcc tgttgctgtt cacgggtcag tgcgttgaag
     7321 cgcacggcgt agccagagac acggattgtc aggttaggat atttttccgg gtgctcgatg
     7381 gcatccagca gcatttcccg attcattacg ttgacgttga ggtgttgacc gccttcgaca
     7441 tccgcttcgt ggtggaaata cccatccagc aggccgacaa ggttggtttt acgtactgga
     7501 tcttctttgc ccagcgccgc aggaacgatt gagaaggtgt acgagatccc atctttggcg
     7561 taggtgaacg gcagtttcgc caccgacgtc aatgaggcca cggcaccttt gcggtcacga
     7621 ccatgcatcg ggttagcgcc cggcgcgaac ggtgttccgg cgcgacgacc gtccggcgta
     7681 ttaccggttt tctggccgta caccacgtta gaagtgatag tcagaatcga ctgggtaggg
     7741 acggcgttgc gataggttgg cagcgcttta attttcttca taaagcgttc aaccaggtcg
     7801 caggcaatgc tgtctacgcg ctcgtcgttg ttgccgtact gcggatattc accgtcgatt
     7861 tcaaagtcca ccgccaggcc gttttcgtca cggattggtt tcacgcgggc atatttgatg
     7921 gcagacaggg agtccgtcgc caccgacagg cccgcgatgc cgcatgccat agtgcgatag
     7981 acatcacgat cgtgcagcgc catcagcgaa gcttcgtagc tgtacttgtc gtgcatgtag
     8041 tgaatgatat tcagcgcgct gatgtactgc accgccagcc agtccatgaa gtgatcgagg
     8101 ctgtccatca ctttgtcgta atccagcacg tcgtccatca gcggtgctgt tttcggcccg
     8161 acctgaatct tcagcttctc gtccaccccg ccgttaattg cgtacagcag tgttttcgcc
     8221 aggttagcgc gtgcaccaaa gaactgcatt tgcttaccaa tcaccattgg gctgacgcaa
     8281 caagcaattg cgtaatcgtc gctgttgaag tcagtacgca tcagatcgtc gttttcatac
     8341 tgcaaggaag aggtgacgat cgacacctgc gcggcatatt ttttgaaggc aatcggtaat
     8401 tcttccgacc aaagaatggt caggttaggt tccggtgccg gccccatagt gtgcagggtg
     8461 tgcaaatagc ggaaggagtt tttggtcacc agcgtacgac cgtccagccc catcccgccg
     8521 atcacttccg tcgcccagat tgggtcgccg gagaacagcg aatcaaattc cggcgtacgc
     8581 aagaagcgca ccatacggat cttcataatg aagtgatcga tcagttcctg tgcctgctgc
     8641 tcattgagta cgcctgcttt aaagtcgcgc tcaatgtaga tatcgaggaa cgacgccgtg
     8701 cgccccagcg acatcgcgcc gccgttctgt gatttcactg ccgccagata agcgaagtag
     8761 agccactgca ccgcttcctg cgcattctgc gccgggcgag agatatcaaa gccatatttc
     8821 gccgccattt cctgaatctg caacagcgca tgacgatgct ctgccagctc ctcacgcaga
     8881 cggatggtgg cttccagatc ttcgcctttt tccagacggg actggagatc ggcaaattgc
     8941 agttcgcgtt cacgcaccag ataactgatg ccatacagcg ctacgcggcg atagtcaccg
     9001 ataatgcgcc cacggccata gccatctggt aaaccggtca gcacgccaga tttacggcag
     9061 cgcagcatat ccggtgagta aacatcaaat acgccctggt tatgggtttt acgcagatcg
     9121 gtaaacagat attcaaattc actgtccatt tcacggccat aggcgtggaa tgaactttta
     9181 atcatattaa tgccaccgaa cggatgcagc gcgcgcttta atggcgcatc cgtttgcaga
     9241 ccaacaattt tttccagcgg ctggttaata tatcccgcat catgagcggt aattgtggtg
     9301 gcaatattgg tatcgaaatc aaccggcgcg tgggttgcgt tttcgatacg gatgccttcc
     9361 attacttttt cccacaattc cgtggtggca ggcgtcgctt cggcgaggaa agattcatcg
     9421 ccttcatacg gtgtatagtt atgttgaata aaatcgcgga cattaatttc gtttttccag
     9481 tccgtacctt taaagccaag ccatgcgtcg gcgtacagct tatcgctggt atcaatatct
     9541 atcttcatga aaaataatct ctctacaata cttcaactaa attatgcaaa ttctgcgggc
     9601 gcgttaactt tgcctaaatg aatggcatcc aaagcaatca ttttttcttc gttagtcgga
     9661 ataacggcac aaatgacacg cgcattttca ctggaaacaa ttcgctctcc aaaagagtta
     9721 gagcgattat tcatttctgt atcaatcact acgcctaata cagccaaatg ttccatgacc
     9781 agacgacgaa ttaaacttga attctctcct attccgccgg tgaatataat tccatccagg
     9841 cgacgtaatg aagctgcgtg tccggcaata tgacgggcaa ttcggtgaac aaaggtttta
     9901 attgccagtt gcgcgcgttc gtgaccttca tgccaggctt tttccagaac acgtaaatcc
     9961 gaagaaagac cggaaatacc taataatccc gactctttat tcactacgcg ttccaggtca
    10021 cccaggctct ggttggtttg gctggcgacc caggacatcg caccaaagtc gacatcgcca
    10081 ctgcgggttc ccatcatcaa gccttccagc ggcgtcattc ccattgaagt atcaacactc
    10141 tgaccgttgc gaaccgcgca gattgacgcg ccattgccaa gatgcgccac aaccaggccg
    10201 gagtcatctt ccgccagatt cagcagagaa tgtgcgcgct gggaaacata gcggtgcgac
    10261 gtgccgtgga aaccataacg gcgtacacct aactcttcat aatatttcca cggcaggccg
    10321 tataaataag cttccggagc catcgtctgg tggaaactgg tatcaaatac cgccacctga
    10381 gttacgcccg gaaataattg ctgcgccgat tcaataccac ttaaattggc gtaattatgc
    10441 aggggtgcca gtggagaaac gcgacggata ttatcaatga cttcatcggt aataatggcg
    10501 gactcggtaa aaatactgcc gccgtgagcg atgcggtggc caattaaggc cacactgtca
    10561 tttaaattcc gtttttccag ttcaaatgca attgccttca atgcaccttc gtagctgtgg
    10621 tgagccagcg gtgctggctc tcccccattt acggataaga atgcattttc cgagttaata
    10681 ccgtcggcaa tacctgacat taatacttca cagtcgctgg catcgagcac ggaaaactta
    10741 atcgaagacg aaccacagtt aataaccaaa acaaccggaa attcattcat ctcttttctc
    10801 atcctgagtt acggattaaa acagtttgta tacgatgttc aggatggtca gcagaccaat
    10861 cacggtaaca aacacgttat ccagacgacc acggtatttc gccagagacg gcgctttacg
    10921 gatggcatac atcggcaaca ggcacagcag ggatgcgata atcggtgcgc ccatggcttc
    10981 aatcaggtcg aggatgttcg ggttggcgta ggcaacaacc caggtggagc ccatgatgaa
    11041 gatcatgctg atagtgttca gtttacccag cgacacttta gttttgtcgc ctttataacc
    11101 aaacttcagg accaggccat tcagaccttc cagcgttccc agatagtgac cgaagaaaga
    11161 tttgaagata gccacgagtg cgatgatgga agccgcatat tccagtgtaa tcgcgaacgt
    11221 tgttttggta ccggtcatgg acgcaaagtg gttagccaga taagaaagca ctggaatatt
    11281 ctgcgctttg gcttccgcca tgttggccgg agacagcgta aataggcagc taaaggcaaa
    11341 gaacatcacc actgcaacca tcagcatgct ggcacgagaa atgatttggg aacatttacg
    11401 ttcggtgaag tcgcgaccga agtctttctc atactcttca cgtttagaaa ccacgaagga
    11461 agagacgatt ggcgagaaat taaaggagaa aaccatgatg gaaatcccca gccagacggt
    11521 gatcaggata ccgtcatgac cggttaacga cagcgaaccg aggtcaacct ggtcgataac
    11581 tgcagagttc cagtaaggga tcagcgacag agaaatcagc accaggctgg cgataaacgg
    11641 ccataccagg tagctcatca ctttaaccat cagatcctta ccaaaccaga tgacgaaagc
    11701 catcagcagc agcaggaaca gcgccacaaa gccgcgattc agcggtgcaa agccgagctg
    11761 gttttcccag aacgtcataa aggtattggt aatagtaacg ccataaatcc acagcagtgg
    11821 gcaaatcgcg aagaagtaca ggaacgtgat aaccacgccg ccagttttac caaaatgctc
    11881 ttccaccgtt tccgtaatgt tgccggaagg gttagagcca gaaagacata gacgcgccag
    11941 cgcccggtgg caataaaatg cgatggggta tgccaatacc aacatcagaa gaatcgggat
    12001 cagtccgcca aaacctgcgc ggatagggaa gaacagcacc ccggcgccga ttgccgtacc
    12061 aaacaagcct aacgtccatg tggtatctga tttacgccag gacgattgtt ttgtctggct
    12121 ggatacaatg ctatctgaag tactcatatc ctatcctcaa cgaattaatt aagcgtcaac
    12181 gaaaccggtg atttgagaga cgcgagaaag atcgatattg ccgccggaaa taatactgac
    12241 ggtttttctg ttttgaatat attggtctaa tttaccgctt aataatgcag cacatgccag
    12301 agcgcctgcg ccttcggtga cgactttatt gcgctgaatt aaggcaatca tactgtttct
    12361 gatttcgtct tcgctgacca gcacgatgtc atcgactaat tcacgaacga tttcgtaagt
    12421 taaattaccc gggcgggaga catcacaacc atccgccagg gtgccggtag ttcggtgcgt
    12481 ggttatttct ccggagtgga aagaagccgc catgccgtga acgttttcag actgtacgcc
    12541 aataacacga atggtcgggt taatagattt aattgccacc gcaataccag caattaaacc
    12601 gccaccacca attggcacaa tcacgttatc gacatcatag agatcttcca taatttccag
    12661 accaatcgtt ccctggccag caatcacttt cggatcatcg taaggtggga taaaaatacg
    12721 gccttccatt tcgacaattt cgctcacttt agcgatagtg tcgttgaagt tatcaccatg
    12781 cagaacgact tctgcggagt agtcgcacgt tgccgctact ttggattttg gcgcaccttt
    12841 tggcatcacc actttaccgt cgatacccag catcgcgcag gagagcgaaa ccccttgcgc
    12901 atggttgccc gcagaacagg ccaccacgcc tttgcgtttt tccgcatcgg tcagtgaact
    12961 taatttatta aatgcgccac gaattttaaa tgaacccgta cgctgcatat tttcaaactt
    13021 caggaatatt tcacctttgc aacgttcact aaaatagttg gagcgaggca tgcctgtttt
    13081 ataaattcgc ccagccagtc gttgtttcgc ttcaataata tcatcaatag caatcggcag
    13141 atcgtatgta atatgcatta taaaacctct tcgcctgaat taaatatgta ggtaaccgta
    13201 accgacacct gcaaaacaga caggtggatt atttatgttt aaataaaaac aaataactaa
    13261 ccaacttcta ttaattgcct tcgtctacac ccattataag atgaatactc tttggctaat
    13321 tccaccaaaa ccgatgctgc ttttttaata cgataatttt tcgaccatac cgcggcatat
    13381 tgtgccacag gtaatgtttc ttcaaccgga atagtaataa attgattaga accaaaaggt
    13441 gacgtcatat cacaaggaat tacagttaag aaatcagcat tgagaacaag attataaatt
    13501 gtcacgactg agtcggtttt aacgatgttt tcaatactga tgccatttct ttgtaacgta
    13561 gtaagcagtt cgctgtagta ccccatatta gtttgtggca acacccactg ttcgttcttc
    13621 aacgactcca gcgtggtggt gccggtgcat gttcgggact tactggctac cagcacaaac
    13681 tcggactcga acagcggctc aacatgtaaa tcctgaagct tcatttctgc acttaatgta
    13741 ccaatcgcaa aatccagtcg accgtcgcgg attgccggta agaacgaaga cagttgcgct
    13801 tcatacatag aaacctgcgc tttcgggaac acctctttga acttgttgat catccctgac
    13861 ataaaagtaa aaccaatcaa tgaaggaaaa ccaaatgaga cttccaccac cgcctcagaa
    13921 gacataccgc ttatctcatt aaccatattt ttcatttcac gggtaatgga ttcggaacgg
    13981 gagagtaaca attgaccggc aggtgttaat gttacaccgg tatttttccg caccactaat
    14041 tccacaccaa aataatcttc aatatcgtta atgattttac tgacggccgg ttgagttaac
    14101 cctaattctt ttgcagccga gccgatagag ccacttctaa tgacttcctg aaagactacc
    14161 aggtgctgcg ttttcggaag aagaatagtg ctcataacga cctacgttaa ttacctcatt
    14221 gacggcatga agtgtatcaa aatgaaatga acaggatatg tgcgaccact cacaaattaa
    14281 ctttcaatac tttccagggt atcgtcatta caattaaata cctttaaata tcaacaagtt
    14341 aaagcataaa aatcagcata aaccctgatt tttgtcaaaa aaaatgacgg ggataaccat
    14401 attttatggc gataacatca t
//
LOCUS       DAZSBF010000098        14306 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZSBF010000098 DAZSBF010000000
VERSION     DAZSBF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.099"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            19..1245
                     /gene="rtcB"
                     /locus_tag="ACHXWD_002331"
     CDS             19..1245
                     /gene="rtcB"
                     /locus_tag="ACHXWD_002331"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR7621234.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHXWD_002332"
     CDS             1249..2265
                     /gene="rtcA"
                     /locus_tag="ACHXWD_002332"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR7621235.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHXWD_002333"
     CDS             complement(2308..5013)
                     /gene="malT"
                     /locus_tag="ACHXWD_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR7621236.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5624..8017
                     /gene="malP"
                     /locus_tag="ACHXWD_002334"
     CDS             5624..8017
                     /gene="malP"
                     /locus_tag="ACHXWD_002334"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR7621237.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8027..10111
                     /gene="malQ"
                     /locus_tag="ACHXWD_002335"
     CDS             8027..10111
                     /gene="malQ"
                     /locus_tag="ACHXWD_002335"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR7621238.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHXWD_002336"
     CDS             complement(10156..11472)
                     /gene="gntT"
                     /locus_tag="ACHXWD_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR7621239.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHXWD_002337"
     CDS             complement(11833..12408)
                     /gene="nfuA"
                     /locus_tag="ACHXWD_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR7621240.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHXWD_002338"
     CDS             complement(12467..13150)
                     /gene="gntX"
                     /locus_tag="ACHXWD_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR7621241.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13188..13958
                     /gene="bioH"
                     /locus_tag="ACHXWD_002339"
     CDS             13188..13958
                     /gene="bioH"
                     /locus_tag="ACHXWD_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR7621242.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13987..>14306)
                     /locus_tag="ACHXWD_002340"
     CDS             complement(13987..>14306)
                     /locus_tag="ACHXWD_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR7621243.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3442 a         3568 c         3996 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgctggga acgggtaacc actttattga aatctgcctt
      541 gatgagtcgg agcaggtgtg gattatgctg cactccggtt cacgcggaat tggtaacgcc
      601 atcgggactt actttatcga tctggcgcaa aaagagatgc aggaccaact tgagacgttg
      661 ccgtcgcgtg atctggcgta ctttatggaa ggtacggaat actttgatga ttacctaaga
      721 gccgtggcct gggcgcagct tttcgccagc cttaaccgcg atgcgatgat ggaaaacgtg
      781 gtaacggcat tgcagagcat tacgcagaaa acggtcagac agccacaaac gctggcgatg
      841 gaagagatca actgtcacca caactatgtg caaaaagaac agcactttgg tgaagagatc
      901 tacgtgacgc gtaaaggcgc ggtgtttgcg cgtgctggtc aatatggaat tattccaggg
      961 tcgatgggag caaaaagctt tatcgtccgt gggctgggaa atgaagaatc tttctgttcg
     1021 tgcagccacg gtgccgggcg ggtaatgagc cgtactaaag cgaaaaaact gttcagcgtg
     1081 gaagatcaaa ttcgtgccac cgcgcatgtg gaatgccgta aagatgccga agtgatcgac
     1141 gaaatcccga tggcgtataa agatattgat gcggtgatgg cggcacaaag cgatctggtg
     1201 gaagttatct ataccctgcg tcaggtggtg tgcgtaaaag gataaatgat gaaaaggatg
     1261 attgcgctgg atggcgcaca gggcgaaggt ggcgggcaga tcctgcgctc ggcgctgagc
     1321 ctgtcgatga taaccggcca gccatttacc atcaccagca ttcgtgccgg gcgggcgaaa
     1381 ccggggctgt tgcgccagca tctgaccgcg gtaaaagcgg ctgcggaaat ttgtagggca
     1441 acggtggaag gcgcggagct ggggtcgcag cgtctggtct tccggcttgg caccgtgcgc
     1501 ggcggcgagt accgctttgc tatcggtagt gccggaagct gtacgctggt gctgcaaacg
     1561 gtgctgcccg cgctgtggtt tgccgatgga ccttcgcgtg ttgaagtgag cggaggcacc
     1621 gataacccgt cggccccgcc tgcggatttt atccgccggg tgctggagcc gctgctggcg
     1681 aaaataggaa ttcatcagca aaccacgctg ttacgtcacg gtttttatcc tgccggaggc
     1741 ggcgtggtgg caacggaagt ctcgcccgtg gcatcgttta acaccttgca acttggcgag
     1801 cgcgggaaca ttgtgcagat gcgtggagaa gttctattag ctggtgtgcc gcgccatgtt
     1861 gctgagcgtg aaatcgctac actggcgggg agtttttccc tgcatgaaca gaatattcat
     1921 aacctgccgc gcgaccaggg gccgggtaat accgtctcgc tggaagtcga aagtgaaaat
     1981 atcactgaac gctttttcgt cgtcggtgaa aagcgcgtca gtgccgaggt ggtagccgcg
     2041 cagttagtga aagaggtgaa acgctacctg gcaagcccgg cggtagtggg ggaatatctc
     2101 gccgaccagc tggtgctacc gatggcgctg gcgggcgcgg gagaatttac ggtcgcccat
     2161 ccttcatgcc atctgctgac caatatcgcc gtggtggagc gtttcttgcc ggtgcggttt
     2221 ggtttgatag aaacagatgg cgtaacgcgg gtgagtattg aatgatgtaa agatgtaaac
     2281 cggatccaat gcattatccg gctaaactca caccccatat cccatcatct tcagcagttt
     2341 ctgcgcgtgt tgtaccgcgg cctgacggtg ggcgacgccg agtttttggt acagattgcg
     2401 gatatgcgtc ttgatggtgg ttgccgccac ttccagctcg cccgcaattt gctcattgct
     2461 gtaaccggag tagatcagcc ccaggacctg ccattcacgt tgggtcaacg ggctggtgtg
     2521 gatcagttct ggcacttccg gatgattcag cagacgttca acgaaattct catcgaaatg
     2581 ggcgaattta tgccgatgat gttgattgat ttctcgcaga atacgctgcg cgcgatgctg
     2641 ttccagttcc ggcagcgtat tgagttgaat cagctggcgc agttgttgcg ccatcgcttc
     2701 gccttcgatg acaaaatgac tgataaatcc ggtgcgattc gccagtttta atgcgtccag
     2761 caacacgcgc tgggcgtcac ttttacgtcc ggcctgccag tacagttgat taagcagcaa
     2821 caggttacgg ttgagatcgc tcatcaaccg cagactgcgg gcattttcat tgagttcttc
     2881 gagaacaatt tccgcaggtt caaactcgcc cagcaagatt tgtgcgcggg caatgttgcg
     2941 ccattgacct tgcaggaagt ggttgttcgc aaactccggt ttagccgtat gacgcaacca
     3001 gttggcagcg gcggctttat cgccggtcat ttgccagtaa atcacccgga ctttgttggc
     3061 gttagagatc cagtcgctgt gatatttgcc attccccagc aggttttcca gacggttcag
     3121 ctggctacgg gcgttatcta aatcaccacg ggccagcgag cattgaatca acattgccag
     3181 gcactgaagc tgttgctgtg gctgataaga cgacaagact tcaatcccgc tacgcgccga
     3241 cgcttcggct tcatccagcc gtgcccaggc ccataacagt tgtgctcgga tgcgcaccag
     3301 aaactcatgc atcggcagct gttccagatg ctgctcgttg atcagctgga acgttttttc
     3361 ctgtgtttcc cacgcggttt gcaggaaccc ttgggcaaac agaatttcac tttgctggat
     3421 taaactccat aaggcgtagt gccagacgtc gtgctggcgt gccatctgtt cggtttgctg
     3481 cattagcgcc agtgagcggg tcaattcgcc tttgcagtgc agcacttcac ccagcaccga
     3541 ggttgctaca atgcggctat agaaccagcc cggtggcagc tcttccagtg ccagttttgc
     3601 cagccgttcc gcttcatccg gattaccatc gttaatcgcc acctgggcgc gcagagcgtt
     3661 aaattctgcg tgcatggtgc cttctctgat gtccttgatt tcatgttcag cacgggctag
     3721 cagggtgtta acttcgccat agcgatgttg gctttgcatt agccacgcct gcaataacac
     3781 taactgcgga ttttccagca aactgtccca cggcagggcc ttaagcgact cttccagcag
     3841 cgacagttcg ctatggttga acaaactcca ggcgtgatta agcagaatat cgcgcagcat
     3901 cagcgcatcg cctgccgcca gcgcatgatg aatcgcttcg ctgggaaatc cctgggccat
     3961 ccagctttct gcggcggcac ggtggatttc cggcagctcc gccgccagtt cccactggca
     4021 gcgctggcgc aggaagttgc caaacagcgg gtgatagcag aaccactcgc cggtatcatc
     4081 catccgctgt aaaaacagcc cctgacgctc aatctcttcg agacgcattt gcccgttttc
     4141 ttcgccagtc acacgggtga tgagggcatc gttcattgag cgcaaaatgg cgcttttcaa
     4201 cagaaaatgg cgcgttgcga gatcgacgtt atccaaaacc tcatcgacca gataatccga
     4261 aagatggctg gcattgattc ccgccaggcg gcgtgccgac ttatgggctg agtgggtatt
     4321 ctgccgggcg gagagggcga ttagctgtag tgccgtcgcc caaccggaaa cgtcatcgca
     4381 aatccaactg ctttctgcgg cttcaatcgg cgatgacaga cggcaatcaa aaaactgctt
     4441 cgcttcctga tgggtaaatg ccagttgctg actgccaatt tccagcagtt ggtcacgaac
     4501 acgcagattg gcaatgccca gttgcggaag gttgcgtgac aacaccacca gggtgagatt
     4561 ttctggttga tggcgaataa agaagcgcat tgactcgtgg atcactggat tagtgatcag
     4621 atgatagtca tcgatgacca gataaagtgg gctatgccat tccgccagct caatgaaaag
     4681 ctgggcgaag agtgacgtca ggctggcata ttgccgtttt tgcgccatcg tctcacatat
     4741 cgcacagtga ccgttggttg cctgctgcac ggcggcaatg agatagctgg cgaaacgctc
     4801 ttgctggtta tcaccttcat ccagcgagta ccagccgata tcgtttttgc ctgccgccca
     4861 ctgggaaatg agggtggtct ttccgtagcc cgcaggactc gtgatcagcg ccagccggaa
     4921 gttgttcgcg ccggaaagtt tagccagcag gcgctcacga accacggtat ggtcgagtcg
     4981 aaccggacga cttagttttg acggaatcag catagttaat cacttcactg tggaaaatga
     5041 ggaaatatta tttttttgcg cttcgtaatt aatggttata aggtcggcca gaaacctttc
     5101 taatgcaagc gatgacgttt ttttatgtgt ctgaatttgc actgtgtcac aattccaaat
     5161 ctttattaac aactcaccta aaacgacgct gatccagcgt gaatactggt ttcccttatg
     5221 ttcatcagat tcatttaagc aagggtttct tcttcattcc tgatgaaagt gccatctaaa
     5281 aagatgatct taataaatct attaagaatg agatggagca cattggatat tttacttgtg
     5341 aaactgtttc actcctttac ttaatttata gagttacctt ccgctttttg aaaatacgca
     5401 acggccattt tttgcactta gatacagatt ttctgcgctg tattgcattg atttgctgct
     5461 aatcctgtgg tttgcactag ctttaagtgg ttgagatcac atttccttgc tcatccccgc
     5521 aactcctccc tgcctaatcc cccgcaggat gaggaaggtc aacatcgagc ctggcaaact
     5581 agcgataacg ttgtgttgaa aatctaagaa aagtggaact cctatgtcac aacctatttt
     5641 taacgataag caatttcagg aagcgctttc acgtcagtgg cagcgttatg gcttaaattc
     5701 tgcggctgaa atgactcctc gccagtggtg gctagcagtg agtgaagcac tggccgaaat
     5761 gctgcgtgct cagccattcg ccaagccggt ggcgaatcag cgacatgtta actacatctc
     5821 aatggagttt ttgattggtc gcctgacggg caacaacctg ttgaatctcg gctggtatca
     5881 ggatgtacag gattcgttga aggcttatga catcaatctg acggacctgc tggaagaaga
     5941 gatcgacccg gcgctgggta acggtggtct gggacgtctg gcggcgtgct tcctcgactc
     6001 aatggcaact gtcggtcagt ctgcgacggg ttacggtctg aactatcaat atggtttgtt
     6061 ccgccagtct tttgtcgatg gcaaacaggt tgaagcgccg gatgactggc atcgcagtaa
     6121 ctacccgtgg ttccgccaca acgaagcact ggatgtgcag gtagggattg gcggtaaagt
     6181 gacgaaagac ggacgctggg agccggagtt taccattacc ggtcaagcgt gggatctccc
     6241 cgttgtcggc tatcgtaatg gcgtggcgca gccgctgcgt ctgtggcagg cgacgcacgc
     6301 gcatccgttt gatctgacta aatttaacga cggtgatttc ctgcgtgccg aacagcaggg
     6361 catcaatgcg gaaaaactga ccaaagttct ctatccaaac gacaaccata ctgccggtaa
     6421 aaagctgcgc ttgatgcagc agtacttcca gtgtgcctgt tcggtagcgg atattttgcg
     6481 tcgccatcat ctggcggggc gtaaactgca cgaactggcg gattacgaag ttattcagct
     6541 gaacgatacc cacccaacta tcgcgattcc agaactgctg cgcgtgctga tcgatgagca
     6601 ccagatgagc tgggatgacg cttgggccat taccagcaaa actttcgctt acaccaacca
     6661 taccctgatg ccagaagcgc tggaacgctg ggatgtgaaa ctggtgaaag gcttactgcc
     6721 gcgccatatg cagattatta acgaaattaa tactcgcttt aaaacgctgg tggagaaaac
     6781 ctggccgggc gatgaaaaag tgtgggccaa actggcggtg gtgcacgata aacaagtgca
     6841 tatggcgaac ctgtgtgtgg ttggcggttt cgcggtgaac ggtgttgcgg cgctgcactc
     6901 ggatctggtg gtgaaagatc tgttcccgga atatcaccag ctatggccga acaaattcca
     6961 taacgtcacc aacggtatta ccccacgtcg ctggatcaaa cagtgcaacc ctgcactggc
     7021 ggctctgttg gataaatcac tgcaaaaaga gtgggctaac gatctcgatc agctgatcaa
     7081 tctggaaaaa ttcgctgatg atgcgaaatt ccgtcagcaa tatcgcgaga tcaagcaggc
     7141 gaataaagtc cgtctggcgg agtttgtgaa agttcgtacc ggtattgaga tcaatccaca
     7201 ggcgattttc gatattcaga tcaaacgtct gcatgagtac aaacgccagc acctgaatct
     7261 gctgcatatt ctggcgttgt acaaagaaat tcgtgaaaac ccgcaggctg atcgcgtacc
     7321 gcgcgtcttc ctcttcggcg cgaaagcggc accgggctac tacctggcga agaatattat
     7381 ctttgcgatc aacaaagtgg ctgacgtgat caacaacgat ccactggttg gcgataagtt
     7441 gaaggtggtg ttcctgccgg attattgcgt ttcggcggcg gaaaaactga tcccggcggc
     7501 ggatatctcc gaacaaattt cgactgcagg taaagaagct tccggtaccg gcaatatgaa
     7561 actggcgctc aatggtgcgc ttactgtcgg tacgctggat ggtgcgaacg ttgaaatcgc
     7621 cgagaaagtc ggtgaagaaa atatctttat ttttggtcat accgtggaac aagtgaaggc
     7681 aattctggcc aaaggctacg acccggtgaa atggcggaag aaagataagg tgctggacgc
     7741 agtattgaaa gagctggaaa gcggtaaata cagcgacggc gataagcatg ccttcgacca
     7801 gatgctgcac agtatcggca aacaaggcgg cgatccgtat ctggtgatgg cggatttcgc
     7861 agcctatgtt gaggcacaaa agcaggtgga tgtgctgtac cgcgaccagg aggcctggac
     7921 tcgcgcggcg atcctcaata ccgcccgctg cggtatgttt agctcggatc gctctattcg
     7981 cgattatcag gctcgtatct ggcaggcaaa acgctaagga agctcgatgg aaagcaaacg
     8041 tctggataat gccgcgctgg cggcggggat tagccccaat tacatcaatg cccacggtaa
     8101 accgcagtcg attagcgccg aaaccaaacg gcgtttgctt gacgcgatgc atcaacgtac
     8161 cgccacgaaa gtggcggtaa cgccagtccc gaatgtcatg gtttatacca gcggcaaaaa
     8221 aatgccgatg gtggtggagg gcagcggcga atatagctgg ctgctgacca ccgaagaagg
     8281 aacacagtac aaaggccatg taacgggggg caaagcgttc aatctaccga cgaagctgcc
     8341 ggaaggttat cacacgctga cactcaccca ggacgaccag cgcgcgcatt gccgggtgat
     8401 tgtcgccccg aaacgctgtt acgaaccgca ggcgttgctg aataaacaaa agctgtgggg
     8461 tgcctgcgtt cagctttata cgctgcgatc ggaaaaaaac tggggtattg gggattttgg
     8521 cgatctcaaa gcgatgctgg tggatgtggc aaaacgtggc gggtcgttca tcggcctgaa
     8581 cccgattcat gcgctctatc cggcaaatcc ggagagtgcc agcccataca gcccgtcttc
     8641 tcgccgttgg ctgaatgtga tttatatcga cgttaaagcc gtcgaagatt tccatcttag
     8701 cgaagaggct caggcctggt ggcagttgcc gaccacgcaa cagacgctgc aacaagcgcg
     8761 ggatgccgac tgggtcgatt actccacggt taccgcccta aaaatgacag cattacgaat
     8821 ggcgtggaaa ggtttcgcgc aacgtgatga tgagcagatg gccgcgtttc gccagtttgt
     8881 tgcagatcaa ggcgacagcc tgttctggca ggcagccttt gatgcgctac atgcccagca
     8941 agtgaaagag gacgaaatgc gctggggctg gcctgcatgg ccagagatgt atcagaacgt
     9001 ggattcacca gaagtgcgtc agttctgcga agaacatcgt aatgacgtcg atttttatct
     9061 ctggttgcag tggctggctt acagccagtt tgccgcctgc tgggagataa gccagggcta
     9121 tgaaatgccg attggcttgt atcgtgatct ggcggttggc gtagcggaag gtggggcgga
     9181 aacctggtgt gaccgtgaac tatattgcct gaaagcgtcg gttggcgcgc cgccggatat
     9241 cctcggcccg ttggggcaga actggggatt accgccaatg gacccgcata tcatcaccgc
     9301 gcgtgcctat gaaccgttta tcgagctgtt gcgtgccaat atgcaaaact gcggcgcatt
     9361 acgaattgac catgtgatgt cgatgctgcg tttgtggtgg ataccgtatg gcgagacggc
     9421 agatcagggc gcgtatgttc actatccggt ggatgatctg ctctcgattc tggcactcga
     9481 aagtaaacgt catcgctgta tggtgattgg tgaagatctc ggtaccgtac cggtagagat
     9541 tgtcggtaag ctgcgcagca gcggtgtgta ctcctacaaa gtgctctatt tcgaaaacga
     9601 ccacgagaag acgttccgtg caccgaaagc gtatccggag cagtcgatgg cggttgcggc
     9661 gacacatgac ctgcctacgc tacgcggtta ctgggagagc ggggatctaa cgctgggcaa
     9721 aaccctgggg ctgtatccgg atgaagtggt actgcgcggt ctgtatcagg atcgcgaact
     9781 ggcgaagcaa gggctgctgg atgcactgca taaatatggt tgtctgccaa aacgtgccgg
     9841 gcataaggca tcgttgatgt cgatgacgcc gacgctgaac cgtggtttgc agcgctacat
     9901 tgccgacagt aacagtgctc tgttaggact acagccggaa gactggctgg atatggccga
     9961 accggtgaat attcctggca ccagttacca gtataaaaac tggcgacgca agctttccgc
    10021 aacgcttgag tcgatgtttg ccgatgatgg cgtgaacaag ttgctgaagg atttggacag
    10081 acggcgcaga gctgcagcga agaagaagta gagtgcggtt gatgccggat gcgatgcatc
    10141 cggcagtcag tgttatcaaa tcaccatatt cagcagcaga cagcctacca gaccgcacac
    10201 cgagataatg gtttccagca tcgaccagga tttgatggtc tcgccgatag tcaggttaaa
    10261 gtactctttg aacagccaga agcccggatc gttcacatga gagaaaatca cactaccgga
    10321 acccaccgca ataaccatca gctctgggct aacacccgtc gttgcaatca gcggtgccgc
    10381 gataccaccc gcagtgattg ccgcaacggt tgcggaaccc agcgcgatac gcagtacggc
    10441 agcaatcgac caggccatca gcagcggaga aatgttggtt tcgtgcatca tggaagcaat
    10501 gtatttgtcc acgccgctgt ctaccagcac ctgcttgaac gcaccgccac caccgatgat
    10561 caacagcatc atcgcaatga ttttgatgga agaaaccagc gtgtcgttaa tctgatccat
    10621 tgaacgacca cggttcagac caaaggtgaa catcgcaatc agcacggcaa tcagcgttgc
    10681 cattaccggg tcaccgagga actccgctac cggcaggaaa gcgtgacctt tcggcaggat
    10741 catttcggca atcgcacgca tcgccatcag cacaaccggc accagagaag tccagacgct
    10801 gacgccaaag ctcggcatct cttcttcgct gaaggttttc gcgctgtaga gaccttccgg
    10861 gattggctta tcgatacctt tcaacacgcg agcgtaaacc ggaccggcga gaatcacggt
    10921 cgggattgcc agaatagtac cgtacagcag ggttttaccc atatcggcat tgaaaatggt
    10981 ggcaatcgca gtcgggcccg gatgcggtgg caggaagccg tgggtcacag acagtgcagc
    11041 cgccattggt acaccaacat acagcagtgg aatattcgcg gaagccgcga tggtaaacac
    11101 cagcggcagc atcagcacga agcccacttc atagaacagg gcaaaaccaa cggtaaaacc
    11161 ggttaacacc accgcccact ggatgcgctt tttaccaaat ttggcaatca gcgtggtggc
    11221 gatacgttgt gcgccaccgc agtctgccag cattttgccc agcattgcgc caaaacccat
    11281 gatcagggca aggctaccga gcgtcccgcc gacaccgact ttgatggagt caataacttt
    11341 atccagcggc attccttgca ttaatccaac agcaagcgcc acgaggacga gagcgatgaa
    11401 gccgttcatt ttgaagcgga tcatcaggag caacaacaag attacaccga tagcaacaat
    11461 gactaatggc atgatttacc tggcctttca tttgttatgg gtaacgtcaa ttttctgacg
    11521 acaaactcta attatcccaa tcgggaacag agatattgcg gcaccacgac tgatacccac
    11581 taaaactaat tattgtagtc agatgtcagg agtatgtttg gtacccatgt gaatgatacg
    11641 ggtaacattt ggcgtttgag aatcaccaga gcggggtaaa tttaaattat gagaggttgg
    11701 tcatattatc gcggggaaac gaagggaggg atttgccaaa gcaatgctgt gccaacgtct
    11761 ggcacatgtt caacgtaggc ccgaaatgac gctttagcgt cgcatcgggc aatctacaaa
    11821 agaggggata acttagtagt aggagtgttc gccgcgctgg tgttcggtga gatcgcgcac
    11881 acctttcagc tccgggaatt cgttcagcag ctgcttctcg atcccttctt tcagcgtcac
    11941 atcgaccatg gaacaaccgt tacagccgcc gccaaattgc agaatggcgt aaccgtcttc
    12001 ggtgatttcc atcagcgaaa cgcgaccacc gtgaccagca agctgtgggt tgatctgcga
    12061 ctgcagcata tactccacgc gctccatcag cggtgcatcg tctgccactt tacgcatttt
    12121 ggcgttcggg gctttcagcg ttaactgaga acccaactgg tcggtaacaa agtcgatctc
    12181 tgcatcttcc aggtatggtg cgcttaactc atcaacatac gcggtcagca ggtcaaattt
    12241 cagggcagtg tcggtggctt ccacggcatc cggcggacaa taagaaacac cacattcagc
    12301 gttaggcgtg ccagggttaa tcacaaatac gcggatttgt gtcccttctt cctgatttgc
    12361 cagcagtttg gcaaagtgcg cttgtgcagc atcggaaata cggatcatag taatggccta
    12421 atagttgact attttagttg gttataatac gcccatcatc gaggctctac aaggttcgac
    12481 aaaggcacca gacctggaca gtcgccgcac cattgcgtaa aagcaactgc gcaatctctg
    12541 cgacggtact tccggtggta acgacatcat ccacaatcac catatggcga ccttgcacgg
    12601 gcaattcaag acgaaaggca tttttcaggt tgcgcttgcg cagccgggca ctgagaaaat
    12661 gctgggtcgc agtggcccgt gtacgtgtga cggcttcgct atcccattgg cagtgcaacc
    12721 agcgtgataa cggctgacac agcaaatcgc tctgattaaa tccccgacgc cagtgacgcc
    12781 gctgccataa cggaacgctg atgatgcgat ccggcaattg caacccggtg gtgcgacgag
    12841 cgtgtaagac ttccaatagt aacagacgtg acagggcgct ggcgatttca ctgcgccggg
    12901 aaaatttaag ctggtggatg agcagactta acggcggcgc atagtcggca accgtgacca
    12961 gtctttgcca gggcggcggt ttttgcaggc agcgaccgca gggaagatgg gagtgtgtgg
    13021 cgggtaatcc acattgtggg cataacgttt tatctgtgcg ggtggcgcgt gaacagaccg
    13081 aacaaatccc ccaatgacct aacgccagtg gcattcggca tagccagcat aatcccggta
    13141 ctgttagcat atgttcatcc ttgtaagtca aaagagaaca atagcggatg aataacattt
    13201 ggtggcagac caaaggtcag gggaatgttc atcttgtgct gctgcacgga tggggactga
    13261 atgccgaagt gtggcgttgc attgacgagg aacttagctc gcattttacg ctgcaccttg
    13321 ttgatctgcc cggcttcggg cgcagccagg gatttggtgc gctgtcactt gctgatatgg
    13381 ccgaagccgt gctgcaacag gcacctgata aagccatttg gttaggctgg agtctgggcg
    13441 ggctggtggc aagccagatt gcgttaaccc atcccgagcg tgttcaggcg ctggtcaccg
    13501 tggcgtcgtc accttgtttt agtgctcgtg acgattggcc ggggataaaa ccggacgtgc
    13561 tggcgggatt tcagcagcaa ctcagtgatg attttcagcg tacagtggag cggttcctgg
    13621 cgttacaaac catggggact gaaacggcgc gccaggatgc gcgggcgttg aagaaaaccg
    13681 ttctggcgtt accgatgccg gaggttgacg tgcttaatgg cgggctggaa atcctgaaaa
    13741 cggttgatct ccgtcagccg ctgcaaaacg tgcccatgcc gtttttgcga ttgtatggct
    13801 atctcgatgg tctggtgccg cgcaaagtgg tgccgatgct ggataaactc tggcctcgca
    13861 gcaaatcata tatcttcgcc aaagcggccc atgcgccgtt tatttcgcat ccggtcgagt
    13921 tttgtcacct gctggtggcg ttgaagcaga gggtgtaggc aacttttgaa atggcgagac
    13981 agaaatttac tggctcgccg cagccaactc ttcttctgac accccggtaa agcgcatgat
    14041 gtccgcaaga gggactccgg attcaagcat tattttggct atatgcaggg ctttggattg
    14101 ttccccctcc tgccgcaatc tttccgcaat tgtcattaaa ctctccttgt gtttcggtga
    14161 acgttcggca acgccgtcga taaaatcgtt aaaacgtacc gcgtcgccgg tttgcagtat
    14221 gtaattaaac agccctttga tttgtctgtc attagcgtat ccactactta ataagcaggc
    14281 catttgctct accagcccca tcaggt
//
LOCUS       DAZSBF010000099        14091 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZSBF010000099 DAZSBF010000000
VERSION     DAZSBF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.100"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHXWD_002341"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHXWD_002341"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR7621244.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHXWD_002342"
     CDS             2273..2662
                     /locus_tag="ACHXWD_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR7621245.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHXWD_002343"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHXWD_002343"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR7621246.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHXWD_002344"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHXWD_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR7621247.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHXWD_002345"
     CDS             complement(4321..4521)
                     /locus_tag="ACHXWD_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR7621248.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHXWD_002346"
     CDS             4687..5232
                     /locus_tag="ACHXWD_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR7621249.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHXWD_002347"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHXWD_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHXWD_002348"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHXWD_002348"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR7621250.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHXWD_002349"
     CDS             complement(6529..7353)
                     /locus_tag="ACHXWD_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR7621251.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHXWD_002350"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHXWD_002350"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR7621252.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHXWD_002351"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHXWD_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR7621253.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHXWD_002352"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHXWD_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR7621254.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHXWD_002353"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHXWD_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR7621255.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHXWD_002354"
     CDS             complement(11316..11969)
                     /locus_tag="ACHXWD_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR7621256.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHXWD_002355"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHXWD_002355"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR7621257.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHXWD_002356"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHXWD_002356"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR7621258.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3433 a         3562 c         3737 g         3359 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    14041 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg a
//
LOCUS       DAZSBF010000100        14085 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZSBF010000100 DAZSBF010000000
VERSION     DAZSBF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.101"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHXWD_002357"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHXWD_002357"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR7621259.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHXWD_002358"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHXWD_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR7621260.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHXWD_002359"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHXWD_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR7621261.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHXWD_002360"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHXWD_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR7621262.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHXWD_002361"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHXWD_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR7621263.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHXWD_002362"
     CDS             5280..5738
                     /locus_tag="ACHXWD_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR7621264.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHXWD_002363"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHXWD_002363"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR7621265.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHXWD_002364"
     tRNA            6790..6874
                     /locus_tag="ACHXWD_002364"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHXWD_002365"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHXWD_002365"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHXWD_002366"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHXWD_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR7621266.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHXWD_002367"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHXWD_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR7621267.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHXWD_002368"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHXWD_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR7621268.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHXWD_002369"
     CDS             complement(10156..13899)
                     /locus_tag="ACHXWD_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR7621269.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         3486 a         3731 c         3370 g         3498 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cgaatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcgacc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaa
//
LOCUS       DAZSBF010000101        13846 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZSBF010000101 DAZSBF010000000
VERSION     DAZSBF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.102"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1731
                     /gene="adeD"
                     /locus_tag="ACHXWD_002370"
     CDS             <1..1731
                     /gene="adeD"
                     /locus_tag="ACHXWD_002370"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR7621270.1"
                     /translation="SRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKG
                     RYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTT
                     VICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQML
                     AWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAG
                     IENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNP
                     WEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLL
                     SDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTP
                     GKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRLAPACGLLGGFGL
                     NEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAG
                     LMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDVEK
                     FAFTTLEVTE"
     gene            complement(1776..3167)
                     /gene="uhpT"
                     /locus_tag="ACHXWD_002371"
     CDS             complement(1776..3167)
                     /gene="uhpT"
                     /locus_tag="ACHXWD_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR7621271.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3305..4624)
                     /gene="uhpC"
                     /locus_tag="ACHXWD_002372"
     CDS             complement(3305..4624)
                     /gene="uhpC"
                     /locus_tag="ACHXWD_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR7621272.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4634..6136)
                     /gene="uhpB"
                     /locus_tag="ACHXWD_002373"
     CDS             complement(4634..6136)
                     /gene="uhpB"
                     /locus_tag="ACHXWD_002373"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR7621273.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6136..6726)
                     /gene="uhpA"
                     /locus_tag="ACHXWD_002374"
     CDS             complement(6136..6726)
                     /gene="uhpA"
                     /locus_tag="ACHXWD_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR7621274.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6801..7091)
                     /gene="ilvN"
                     /locus_tag="ACHXWD_002375"
     CDS             complement(6801..7091)
                     /gene="ilvN"
                     /locus_tag="ACHXWD_002375"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR7621275.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7095..8783)
                     /gene="ilvB"
                     /locus_tag="ACHXWD_002376"
     CDS             complement(7095..8783)
                     /gene="ilvB"
                     /locus_tag="ACHXWD_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR7621276.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8889..8987)
                     /gene="ivbL"
                     /locus_tag="ACHXWD_002377"
     CDS             complement(8889..8987)
                     /gene="ivbL"
                     /locus_tag="ACHXWD_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR7621277.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9552..9641
                     /gene="tisB"
                     /locus_tag="ACHXWD_002378"
     CDS             9552..9641
                     /gene="tisB"
                     /locus_tag="ACHXWD_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR7621278.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9765..9839)
                     /gene="ysdE"
                     /locus_tag="ACHXWD_002379"
     CDS             complement(9765..9839)
                     /gene="ysdE"
                     /locus_tag="ACHXWD_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR7621279.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            9921..11105
                     /gene="emrD"
                     /locus_tag="ACHXWD_002380"
     CDS             9921..11105
                     /gene="emrD"
                     /locus_tag="ACHXWD_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR7621280.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11113..11610)
                     /locus_tag="ACHXWD_002381"
     CDS             complement(11113..11610)
                     /locus_tag="ACHXWD_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR7621281.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11607..11969)
                     /locus_tag="ACHXWD_002382"
     CDS             complement(11607..11969)
                     /locus_tag="ACHXWD_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR7621282.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(11959..12306)
                     /locus_tag="ACHXWD_002383"
     CDS             complement(11959..12306)
                     /locus_tag="ACHXWD_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR7621283.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12415..12864
                     /gene="yidI"
                     /locus_tag="ACHXWD_002384"
     CDS             12415..12864
                     /gene="yidI"
                     /locus_tag="ACHXWD_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR7621284.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(12911..13069)
                     /locus_tag="ACHXWD_002385"
                     /pseudo
     CDS             complement(12911..13069)
                     /locus_tag="ACHXWD_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13113..>13846)
                     /locus_tag="ACHXWD_002386"
     CDS             complement(13113..>13846)
                     /locus_tag="ACHXWD_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR7621285.1"
                     /translation="YISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNS
                     FQKNQKKLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNH
                     AMIVIEDLKVSNMSKSAAGTVSLPGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRG
                     GQVLAVPPAYTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAV
                     LACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3239 a         3859 c         3625 g         3123 t
ORIGIN      
        1 agccgggctg aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat
       61 attattgata atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt
      121 gtgattaaag gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg
      181 cagcggattg atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat
      241 attgaatcca gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg
      301 acgaccgtta tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc
      361 tggtttgccc gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc
      421 gtacccgccc tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc
      481 gcctggcggg accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta
      541 attagcgggc agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac
      601 ggtcactgcc cgggtttggg gggtaaagaa cttaacgcct atattactgc gggtattgaa
      661 aactgccacg aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg
      721 ttgatgatcc gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac
      781 gaatttaaca gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc
      841 catgaaggac acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg
      901 catgtggcat atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc
      961 ggcttactgg cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc
     1021 acggtgcagc aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa
     1081 gagtcggcga gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca
     1141 gtttccgcca gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac
     1201 gtcatccata acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt
     1261 gatcgcgatg atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg
     1321 gcttgtggtt tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc
     1381 catgacagcc ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc
     1441 aatcaggtga ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt
     1501 catctgccgt tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa
     1561 caaattgacg ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt
     1621 attcagatgg cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg
     1681 ctatttgatg tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc
     1741 ccggcgtcat gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt
     1801 tttttctcgc ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata
     1861 caaccaatcg cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat
     1921 accggcgtcc catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg
     1981 taagcaaagg tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca
     2041 acagcagcca caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga
     2101 gaagccagat agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc
     2161 agcgccagcg cgatgcaggc caccaggcca cggcgaccgt tcgccaggtc agagagccag
     2221 ccccacagca gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc
     2281 accgctttag agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca
     2341 atacgtacca catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc
     2401 agcacatact caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc
     2461 tcttcgctga tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg
     2521 gagtcgctgc cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag
     2581 ataaacatgc cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct
     2641 gctgcgcctg caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt
     2701 ttacgacgcg gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg
     2761 aaaaagccgc ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc
     2821 atactggcac tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat
     2881 tgtttggtgt ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg
     2941 atggagaaac ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc
     3001 atgtcgttct gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata
     3061 aagaccacca ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc
     3121 ggaaggtcca gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga
     3181 aatgaatacc tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag
     3241 cgtccaggca aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga
     3301 tgcatcacgc ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa
     3361 tcccggcggc gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt
     3421 tcgccagcgg ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg
     3481 tcgccgcccc tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct
     3541 gtgggccaaa gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg
     3601 caaatggcat cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat
     3661 tcatcggccc tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac
     3721 cgataaatcc acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc
     3781 ccagcgtctc ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga
     3841 ccagcacata gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga
     3901 ggatctcttt acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt
     3961 gtcgccattc accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc
     4021 agagaaaaat tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc
     4081 cgtaatgtag tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg
     4141 ccgtgttcca taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca
     4201 gacgcgcaca caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg
     4261 caaacgccca tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa
     4321 gccctatccc cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact
     4381 tcgacacgcc ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga
     4441 gcacgccgtt agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa
     4501 acagcgcgta acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg
     4561 catcaatttc atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca
     4621 acatcatcaa accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca
     4681 gacaggaaat gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc
     4741 caaaaccttg ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca
     4801 tcaaccgttc atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca
     4861 caatgttgtt cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta
     4921 acgctgattc atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca
     4981 tttcccgcat cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac
     5041 gtaaccgacc caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga
     5101 gttgcccgct ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct
     5161 gagtacgaat agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac
     5221 ggcgcacgct ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt
     5281 ccttttgcag cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc
     5341 ctgtcagact ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt
     5401 gactggcgat cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat
     5461 agtgccaggc cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt
     5521 cgtccggcaa tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca
     5581 gatgtcgccc gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg
     5641 tgttattggc gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag
     5701 ttaaagtcag caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact
     5761 gcaacaacgc cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct
     5821 gatggcgata gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca
     5881 ataacggaaa atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg
     5941 cacccagcaa tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac
     6001 caaacggaaa taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc
     6061 acaggcaaaa ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg
     6121 agaacaacgt cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact
     6181 gacgcccagt ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc
     6241 cagttcggcg gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg
     6301 ttcacgtttg gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt
     6361 cagataacag ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca
     6421 gcgtttggaa agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact
     6481 gtcgtgaacg gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag
     6541 accggagata tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag
     6601 ccccgccagc gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag
     6661 ccccagcagc tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt
     6721 gatcatggtc ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat
     6781 gttgttcaag ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga
     6841 tcggactgat tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc
     6901 tgctccagac gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga
     6961 atcggcagac aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg
     7021 tgggtcatta cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt
     7081 gtgttttgca tggcttattc ccccaccatt tcagtattcg ccgcacctgg cggcaccatc
     7141 gggtaaactt tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg
     7201 atgatttcct gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg
     7261 ccgaatccgg cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg
     7321 ccttgctcgt agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc
     7381 agaatgattt tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc
     7441 atcaggctgc cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg
     7501 ccaatcgccg caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg
     7561 cgtgggcgat tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg
     7621 gtgataatcg cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt
     7681 aatggatcgc acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac
     7741 tggtgccact ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca
     7801 tcaacatccg cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg
     7861 atatcgacat gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg
     7921 tcatcaaaac gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta
     7981 ctgcgcacgc cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg
     8041 cccagcgcca ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc
     8101 cgcgcaggcg cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta
     8161 atcattgtag ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct
     8221 gccaccgcgg gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct
     8281 atccacaccg ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc
     8341 gggagttctt cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg
     8401 taggtgtcga cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg
     8461 atgcaaatca gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc
     8521 gcacccggtc cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc
     8581 tgagcgataa agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt
     8641 tggcttaagg catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta
     8701 atgccctgct gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc
     8761 aatgttgtgc ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag
     8821 gtgcttaaac gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt
     8881 tccagtccct acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg
     8941 gatggcgcag ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt
     9001 ggttttgtgt ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt
     9061 tttcagcaag ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa
     9121 aaccccgccg gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt
     9181 attatgtcaa cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt
     9241 ttgacgtatt tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattgc
     9301 tgtttattta tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc
     9361 gcatcgttac ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac
     9421 cccgcgtgag cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt
     9481 gcacaacagc tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac
     9541 aggagacgcg tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag
     9601 cactgcaact gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc
     9661 aaaggggagc gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg
     9721 atgctgacgt atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt
     9781 ttacatattc tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc
     9841 aatgtgatag attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt
     9901 ttataaaagc atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg
     9961 tattactcgt ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata
    10021 tggcgcgcga tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc
    10081 tgacttacgg tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac
    10141 cggtgatcct cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct
    10201 ccagtttgac ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg
    10261 gcgtaatggc gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta
    10321 acagcctgtt aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc
    10381 tgctggatac gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg
    10441 gtgtgacctt cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca
    10501 cgcgcctgct taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc
    10561 tgatgctgat tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga
    10621 tgggcgcggt gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc
    10681 cagcagcgtt ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa
    10741 tgtggcagtc ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt
    10801 ttggcgtgat gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg
    10861 ggatgctgtt tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca
    10921 cggctggcgc gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct
    10981 ctgcgatgtt gccgcaaacc ggtcagggca gcctagggtt gttgatgacc ttaatgggat
    11041 tgttgatcgt gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg
    11101 tttaagcgca cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat
    11161 cagatcacgc atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc
    11221 acaaagcacg ctgtctggcg tctcctgccg agcgtgcggg cacaggcgat gccagtcgca
    11281 cgcctggcag tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc
    11341 gtcaaaaagc tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc
    11401 aattaaaaat ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc
    11461 atcaaattca agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg
    11521 ataaaaatcg gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc
    11581 ttcagcatca atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga
    11641 tgtatatgcg ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat
    11701 ttgtcacgga ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga
    11761 ttacgggtgt agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag
    11821 aataacatgc ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag
    11881 cccagcatgg tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca
    11941 atgcgtctgg ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac
    12001 gaccatcaat attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag
    12061 acgcatcgct ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga
    12121 aaacagacac aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc
    12181 aagttgatca agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag
    12241 aaaagtacgc tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat
    12301 tttcattgtt gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga
    12361 atttcgtccg cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt
    12421 atcatagcgc aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg
    12481 atggggatta tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc
    12541 atcgtggcgg acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa
    12601 aaaattacca ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc
    12661 ggtatcgcac tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa
    12721 aatcgctggg gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc
    12781 ttgctttttg gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc
    12841 ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg
    12901 ggcattatcg tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc
    12961 cggtgtcata gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc
    13021 ccatccagcg cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc
    13081 cacgccctga aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg
    13141 ggttcctgct tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc
    13201 cccgccgcta aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc
    13261 tggcatctga atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg
    13321 caacgctggc ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc
    13381 ttgtacgcaa gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa
    13441 cctgattttg cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac
    13501 atgttgctga ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacggtcgtt
    13561 gtgactttgt gaaggtagtc cctgcggata tttgcgatac aggaatgcag tcgctgtatt
    13621 ttgcgtttct gcttctgcca gttgttgctg aatttgacct tgcggcttaa ctgtcgctga
    13681 agtctcgcca gcttcttctg gtttttctga aaactgttta caggctcaaa gactgtgcca
    13741 tctgacagcg tggcgagttt agccacgcca gcatccagcc cgaccattga tgctgaaggg
    13801 tgaaccggag ttgatacttc actttctgtc tgaatactga tgtacc
//
LOCUS       DAZSBF010000102        13840 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZSBF010000102 DAZSBF010000000
VERSION     DAZSBF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.103"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            178..1431
                     /gene="kch"
                     /locus_tag="ACHXWD_002387"
     CDS             178..1431
                     /gene="kch"
                     /locus_tag="ACHXWD_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR7621286.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1486..1659)
                     /locus_tag="ACHXWD_002388"
     CDS             complement(1486..1659)
                     /locus_tag="ACHXWD_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR7621287.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1802..3262
                     /gene="cls"
                     /locus_tag="ACHXWD_002389"
     CDS             1802..3262
                     /gene="cls"
                     /locus_tag="ACHXWD_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR7621288.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3297..3626
                     /locus_tag="ACHXWD_002390"
     CDS             3297..3626
                     /locus_tag="ACHXWD_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR7621289.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3679..4683)
                     /gene="oppF"
                     /locus_tag="ACHXWD_002391"
     CDS             complement(3679..4683)
                     /gene="oppF"
                     /locus_tag="ACHXWD_002391"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR7621290.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4680..5693)
                     /gene="oppD"
                     /locus_tag="ACHXWD_002392"
     CDS             complement(4680..5693)
                     /gene="oppD"
                     /locus_tag="ACHXWD_002392"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR7621291.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5705..6613)
                     /gene="oppC"
                     /locus_tag="ACHXWD_002393"
     CDS             complement(5705..6613)
                     /gene="oppC"
                     /locus_tag="ACHXWD_002393"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR7621292.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6628..7548)
                     /gene="oppB"
                     /locus_tag="ACHXWD_002394"
     CDS             complement(6628..7548)
                     /gene="oppB"
                     /locus_tag="ACHXWD_002394"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR7621293.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7634..9265)
                     /gene="oppA"
                     /locus_tag="ACHXWD_002395"
     CDS             complement(7634..9265)
                     /gene="oppA"
                     /locus_tag="ACHXWD_002395"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR7621294.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9549..9845
                     /locus_tag="ACHXWD_002396"
     CDS             9549..9845
                     /locus_tag="ACHXWD_002396"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621295.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10003..10650)
                     /gene="ychE"
                     /locus_tag="ACHXWD_002397"
     CDS             complement(10003..10650)
                     /gene="ychE"
                     /locus_tag="ACHXWD_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR7621296.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11128..13803
                     /gene="adhE"
                     /locus_tag="ACHXWD_002398"
                     /gene_synonym="adhC"
     CDS             11128..13803
                     /gene="adhE"
                     /locus_tag="ACHXWD_002398"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR7621297.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3639 a         3423 c         3222 g         3556 t
ORIGIN      
        1 tgatgtgagc atattcagga aaaatgacct ttcctgaggt acagttgctc gctcaaatcc
       61 tttgattact gcggataaag tcatctacta ttgtttaaca cattcacgcg ctgcatgtat
      121 gttcagaaaa tgtgcacgct aagataatta aacaaaattg tggttacaga ggtatcagtg
      181 agtcactggg ctacattcaa acaaacagca acaaatttat gggtgaccct tcggcacgac
      241 attctcgcgc tggccgtctt tttaaatgga ttgcttattt ttaaaacaat ctatggtatg
      301 tcggtcaatt tgcttgatat tttccatatc aaagcatttt cagagttgga tctctccttg
      361 ctggcaaacg ccccactatt tatgctcggc gtctttcttg tcctgaactc cattggctta
      421 ctgttccggg caaagctcgc atgggcaatc agtatcattt tgttgttgat agcgctaatt
      481 tacaccctgc atttttatcc ctggctgaaa tttagtattg gattttgcat ttttacgctg
      541 gtgtttttgc tgatactgcg caaagacttc tcccacagta gcgccgcagc cgggacaatt
      601 tttgcattta ttagtttcac gacattactg ttttactcaa cctacggtgc gctttattta
      661 agcgaaggtt ttaatccgcg aatagaaagt ttgatgaccg cgttctattt ttcgatagaa
      721 accatgtcaa ccgtcggcta tggcgatatt gtccctgttt ctgaatcagc acgattgttc
      781 actatttcgg tcattatttc cggcattacc gtttttgcca catctatgac ctcaattttt
      841 ggtccgctta tccgcggggg attcaacaaa cttgtaaaag gaaacaatca tacaatgcat
      901 cgtaaagatc attttattgt ttgcggacat tcgattctcg ccatcaatac gatcctgcaa
      961 ctgaatcaac gcggacaaaa cgtaacggtt atcagcaact tgcctgaaga tgatatcaag
     1021 caacttgagc aacgcttagg cgataacgct gatgttatcc ccggtgacag taatgacagt
     1081 tcagtattaa agaaagcggg aatcgatcgt tgccgggcca ttctggcgct gagtgataac
     1141 gatgcagata acgcgtttgt tgtactctcg gcaaaagata tgagcagtga tgtcaaaaca
     1201 gttctcgccg tcagtgatag caaaaacctg aataagatta agatggtaca tccggatatc
     1261 attctctcac cgcaactgtt tggcagcgaa attctggcgc gagttttaaa tggtgaagag
     1321 attaataatg atatgctcgt ttcaatgttg ttgaactccg gtcatggcat tttcagcgat
     1381 aacgatgaac aagaaacgaa agctgacagt aaagaatcag tgcaaaaata gagtttaaag
     1441 acgaaagagt aaacttattg ggcaccgcaa tggtgcccag tatatttaga tattgtagat
     1501 cgcaaacaat aacgagttac gctgtttgtt tagaatgcac ttcctgatgc tgtggatacg
     1561 catatttcgg cgcgattgcc cctcaagcca acgcgattta cgtcggctag cctgacgctg
     1621 catgcgccag cgccccactt ccgttctact ccgcttcatg ttaactactc tatgcaataa
     1681 cagaatggtc attatacccc cacccccgac gctgaccagt gcttttcccg cgtttttatt
     1741 tgccagatga atccatatgc gtaaactcat aacaatgcgc tttcaaaagg atttctaatc
     1801 tatgacaacc gtttatacgt tggtgagttg gttggccatt ctgggatact ggttgctcat
     1861 tgcaggcgta actttacgca ttctaatgaa acgacgcgca gttccctccg cgatggcctg
     1921 gctgttgatt atttacattc tgccgttagt cggaattatt gcctatcttg ccgttggcga
     1981 gctccattta ggcaaacgcc gcgctgagcg cgccagagcg atgtggcctt ccaccgcaaa
     2041 atggcttaac gaccttaaag cctgtaagca tatcttcgcc gaagaaaata gcagtgtcgc
     2101 tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc gctggggtca aagggaatca
     2161 gctacaactg atgaccgagt cagatgatgt gatgcaggcg ttaatccgcg acatccagct
     2221 cgcgcgccat aatattgaga tggtgtttta tatctggcag cccggcggca tggcggacca
     2281 ggtggctgaa tcattaatgg cggctgcacg gcgtggtatt cattgccgat tgatgctcga
     2341 ctccgccggg agtgtggctt ttttccgcag cccgtggccc gagctaatgc gtaatgccgg
     2401 tattgaagtg gtcgaagcct taaaggtcaa tctgatgcgt gtgtttttac gccgtatgga
     2461 cctgcgccaa catcgcaaga tgatcatgat cgataattac atcgcgtaca ccggcagcat
     2521 gaatatggtc gatcctcgct acttcaaaca agatgcgggc gtagggcaat ggattgatct
     2581 gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg attatttatt cctgcgactg
     2641 ggagattgaa accggaaaac gtattctgcc gccaccacca gatgtcaata ttatgccgtt
     2701 tgaacaggcc agcggtcaca ccattcacac aattgcttct ggccccggct ttccggaaga
     2761 tctcattcac caggcattat tgactgcggc ttattcggcg cgtgaatatt tgatcatgac
     2821 aacgccctac tttgtgccaa gcgatgattt acttcatgcg atttgcacgg cggcgcagcg
     2881 cggggtggat gtcagtatta tccttccgcg aaaaaatgac tcgatgctgg tcggctgggc
     2941 cagtcgcgca ttctttacgg aactgcttgc tgctggggtt aaaatttatc agtttgaagg
     3001 cgggttactg cataccaaga gcgtgctggt cgatggcgaa ctaagtctgg ttggcaccgt
     3061 taaccttgat atgcgtagtc tgtggctgaa tttcgagatt accctggcaa tcgacgataa
     3121 aggttttggt gcagaccttg ccgccgttca ggacgattat atttcgcgtt cacgtctgct
     3181 cgatgcccgt ttatggctaa aacgtccatt atggcaacgt gtcgccgagc gactgtttta
     3241 cttcttcagc ccgttgctgt aaaacgtcgc caacagaggt taaacaggta gtgactatgg
     3301 atatggatct aaacaatcgc ctgactgaag atgaaacgct tgagcaggcc tacgatattt
     3361 ttctcgaact ggctgccgac aatctcgacc cagctgatgt tctgctgttc aatcttcagt
     3421 ttgaagagcg cggcggcgcg gagttattcg atccggcgga agactggcag gaacatgttg
     3481 atttcgattt gaacccggac ttttttgccg aagtggtgat tggtctggcg gacagtgagg
     3541 atggtgaaat caacgatatt ttcgcccgca ttctgttatg ccgcgaaaaa gatcacaaac
     3601 tttgccatat catctggcgg gaataaataa tcgctttcgg cgtgaaaaag ggctgacaac
     3661 tgtcagccct tattgtgttt aaagcggatc gactttcagg caagaaacgg cgtgtctgaa
     3721 actcccttcc agcacaggac gtgttttggc gcactccgga ccggcaatcg ggcaacaggt
     3781 acggaaaaca caaccggaag gcgggttgat cggcgacggt aattcccctt ccagtaactg
     3841 gatggttttg ttcttctcca gatccggatc aggtatgggg actgccgaca tcaatgccct
     3901 ggtgtaagga tgtagtggat tgtggtagac ctcatcatag gtccccagtt ctaccgcatg
     3961 gccgagatac atcaccaaca cacgatcgga aatgtgtttt accacggcca ggtcatgagc
     4021 gataaaaatt aatgacaatc ccatctcacg ttgcagctgc tggagcaggt tgaccacctg
     4081 cgcctgaatt gacacgtcca gcgccgaaat cggctcatcg cagataatca gcttcggttc
     4141 aagaataaga gcacgagcaa tcccgatacg ctggcactgc ccaccggaga actcatgcgg
     4201 atagcggtta atcaggttag gcaataaccc gactttcagc atcatcgcct tcacgcgctc
     4261 gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc agtggttcag cgatgatctc
     4321 gccgatggtc atacgcgggt ttagcgatgc caacggatcc tggaaaatca tctgaatatc
     4381 actgcgaacg gcacgccatt catcgggctt catgcccagc aactctttac ctaaccaggc
     4441 aacatgaccg ccggtcgctt taaccaggcc aatgatggcg cgagcaaagg tggacttacc
     4501 gcatcccgat tcccctacca cacctaatgt ttccccttca tacaggcgaa gcgtgacacc
     4561 atcgacggct ttgagagttt tcggcggttg ccagaaccac tgtttgccat ctttgatttc
     4621 aaagtgcact ttcagatcgg cgatttcgag gaggactttt cttccttcgg ttacagcatt
     4681 cataacagtt cctccaccgg tttaaagcaa gcacgcagac ggccaggcgt aaactcttcc
     4741 agcggcggtg cgctactaca aatttccatc gcatgcggac aacgtggctg gaacgggcaa
     4801 cctttcggta atcgcagcag gtttggcgga ttaccaggga tggtcaacat tgtttcacct
     4861 tccgcatcga gacgcggcac cgcgttgagc aaaccgatag aataaggatg aacgggttga
     4921 taaaagacat cgcgcgcgtt gccatattcc atcgtgcgcc cggcgtacat taccagcact
     4981 ttgtcgcaga tccccgccac cacgccaaga tcgtgggtaa tcataatgat ggcggtatta
     5041 aattcccgct tcagttcatt taatagcgtc atgatctgcg cctgtacggt gacgtccagc
     5101 gcagtagtgg gttcatccgc aatcagcagc ttaggtcgac atagcaatgc catcgcaatc
     5161 ataactcgct gacgcatgcc accagaaaat tcgtgcgggt acattttcat gcgtttacgt
     5221 gcttccggca tttttaccgc atcgagcatc cgcaccgact cttcaaacgc ctcagctttg
     5281 ctcatgttct tatgcagcat cagcacttcc atcaactgct caccgacgcg catataggga
     5341 ttcaacgaag tcattgggtc ctgaaaaatc attgagattt gttcagcgcg cagtttattg
     5401 agttcacgct ctggcaaatt gaggatttca cgcccattga aggttgccga tccgccaata
     5461 cgtccgttgg cagccaacag gcccatcaac gcaaatgcag tttgcgattt acccgaacca
     5521 gactcaccta caatgcccag cgtttctccg gcacgtagag aaaaattcaa atcattgacc
     5581 gccgtgacat cgccgtccgg ggtactaaag gtgacacgca aatctttcac gttcagcagt
     5641 acgtcagcct gttgttgtgc gagcggcaca gttgcagttt caattacgct catggctgca
     5701 ctccttaacg atctttcggg tcgagagcat cacgcaagcc atcgccgata aagttgaaac
     5761 aaaacagcgt caccacgagg aatcccgctg ggaacaacaa taaccatgga gagacttcca
     5821 tcgagttcgc gccatcactc agcaatgcac cccagctgct taacggctct tgcgtaccca
     5881 accccaggaa gctaaggaaa gattcaaaga ggatcatgct gggcaccaga agtgatgcgt
     5941 agaccaccac cacaccgagt acattcggca caatgtggcg aataacaatg cccgatgtcg
     6001 atacaccgcc aacttgtgcc gcctcaataa actctttgcg cttcagactc agggtttgtc
     6061 cacgcacaat acgagccata tccagccagg aaaccatgcc aatcgccacg aaaatcagca
     6121 ggatgttttg accgaaaaag gtcacaagca aaatgacgaa gaacatgaat gggaaggagt
     6181 tgaggatttc cagcagacgc atcattaccg aatccacttt accgcccaga taaccggaaa
     6241 gcgaaccgta aagtgtcccc acgaccactg ccaccagtgc cgcagcaaca ccgaccatga
     6301 gtgagatacg cccgccaatc gcaacgcgca caagcaggtc gcgaccggat gagtcagtac
     6361 caaagtagtg accggactcc atatccgggg cgctggacat catcgcccag tcagtatcgt
     6421 cataggcaaa ctgcgaaagc atcggtgcca ggattacaaa taacgcgatc agcaccagca
     6481 ctatcagact ggcaaccgcc gcacggttat gcataaaacg tcgacgtgcg tcctgccaca
     6541 agctgcgccc ttcgacctcc agcttttcac tgaaattttc cagcgtctcg ctgtttttct
     6601 tacttaacat catcgcgagc tccagtatca gtaacggatt ttcgggtcga taaccgcata
     6661 tagcacatcg acaatggcat taaacaaaat ggttaaagca ccaaccagga tggtcaggct
     6721 taacactaag gaatagtcac ggttcaatgc gccattaacg aacagttgtc caatccccgg
     6781 caaaccataa atggtttcga taaccataga accggtaata atgccgacaa atgcggggcc
     6841 catataagag agcacgggta acagagcagg ttttaatgcg tggcgtaaaa tgatccgccg
     6901 cataggtaac cctttcgccc gggcagtacg aataaagttg gagtgtaata cttcaatcat
     6961 agagcctcgg gtaatacgcg caatactggc gatataagcg agtgacaacg ccaccatcgg
     7021 caatatcatg aatttaagcg ccccaccatt ccagccaccg cccggcagcc aatgcaaaat
     7081 gatcgcaaat atcatgacta ataatggcgc aaccacaaaa ctggggataa caaccccggt
     7141 cattgccagc cccatcacgg tatagtccca tttggtgttt tgttttaatg cgacaataac
     7201 gccagcacta acacccagta ttaccgcaag gaaaaatgcg gcggctccca gtttggcaga
     7261 aacgggaaaa ctggatgcaa ccaggtcatt gaccgaataa tctttatatt taaacgatgg
     7321 accgaaatca ccgtgcgcca gttgtttcag gtagctgaaa tactgtgtca tgatcggatc
     7381 attaagatga tatttcgctt cgatattggc catcacttcc ggcggtaaag tacgttcgcc
     7441 ggtaaaaggg cttcccggcg cgaggcgcat cataaagaac gaaatagtaa taagaataaa
     7501 tagcgtcgga atcgcttcca gacagcgacg tagaataaat tttaacattg ccctaccttc
     7561 tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg tggagcagga cactcctgcc
     7621 ccacgtattg ccattagtgc ttcacaatgt acatattccg ggtgtaggta ttatccagcg
     7681 gatctttgcc ggtatagcca ccaacccacg gtttcaccag acgcgcattc acgtagtaat
     7741 aaacaggaac aatggccgaa tccttatcca gctgttgttc tgctttagtg tacagagctg
     7801 tgcgctgcgc ctcgtcagtc actttcagcg tttccgccat aatgctgtca aaggccgggc
     7861 tcttataatg cgcggtattc atcgagctgt tcgacagcat ggtgttcagg aaggaagttg
     7921 gttcgttgta gtctgcacac cagcctgcac gggccacatc aaaagtgccc tggtgacggg
     7981 tgtcgaggaa cgttttccac tcctggttaa ccagtttgac gtttacacca atgtttttct
     8041 tccacaatga agaggcagca atcgccagct ttttatgcag atcagaggtg ttatacagca
     8101 ggttgatggt caacggtttg tctgcggtat aacccgcttc agccagcagt tttttcgctt
     8161 cttcgttacg tttttcctgg ctccagccaa accattctgg ctgagtcaat tttgcgccat
     8221 cagtatacgg tggagtgtaa ccataggcgg gcatgtcgcc ctgcgctttc actttattaa
     8281 caatgatatc gcggtccata cctagtttca gtgcggtacg cacacgcaca tcgttgaatg
     8341 gcggtttctg gttgttaatt tcgtaatagt aagtgcacag gtatggatca acgtgaactt
     8401 cgtccgggat ctcttttttc agcttctgga acaattcgat cggcatgttg ttatacgtca
     8461 tgtcgatttc accactacgg tagcggttga catcggtaac ttcagaagca ataggcaaat
     8521 aggttacctg gttaataacg gttttcgcgt tgttccagta ggtcgggctg cgttcaagaa
     8581 cgattcgttc gtttacgacc caatctttta aggtataggc accgttggtg acgatattac
     8641 caggctgggt ccatttttcg ccgaatttct cgatagcggc ttttggcacc ggtgaagttg
     8701 atgggtgaac aagtaattta tagaagtacg gaacgggttc acttaaggtg acttctaatg
     8761 tgtgatcatc aatagctttc acgccgagat cggtaatcgg ttttttccct tcaagaattt
     8821 catcaatacc ggcgatatgc ccatattgca gataactggc atacggagaa gcagtgttcg
     8881 gatcaacaga acgttgccag ctatacacaa agtcttgtgc tgtgactggc gtgctatcag
     8941 accatttcgc atctttacgc aaatggaagg tccagacttt cgcgtcttta ttatcccagg
     9001 attcagcgac gccaggtgct ggatgaccgt caagatcgct gaccagtaag ccttcaaaca
     9061 ggtctcggct gatattagac tccggaacac cttcaatttt gtgcggatct aatgactgaa
     9121 cttctgaacc attgttacgt accagtgttt gtttttccgc cagtgtgacg cctgcgggta
     9181 catcagctgc cagcgcgaca ttccctgcca ttagcgcagc cagaacgcca gctgctacta
     9241 aacttctctt ggtgatgttg gtcattgttt tttggactcc ctcattataa ttactggtat
     9301 taaccagcat gtgtaatccc ggattgggac cctgcacagg ctcggagact ttctgctgtc
     9361 aggttacttt atcgttacgt attactatca ccgactttat ttattctggt gattcagacg
     9421 atcaccttat gtcgattcgt actgtgtctc ccctgtcgag acacattatt ctttcgcgac
     9481 gctctttaat tgttaattat tctcattacc aataaagaat cgtctggcgg ttgcccggaa
     9541 agtaccaaat gcggttctta ctcgccaata cattttgcaa aattgttaag caattctctt
     9601 ttattgaccg tatcagaatc tatagcttgc atccatgcct taacaaacat ctttcaattc
     9661 aatcaaatag agattggcta agcatgtcta tcttttctga ttacagttcc agtagtgaaa
     9721 tgcacaataa tttaacaatt gattattatt tagcactttc ctctaccaaa ggcagtggaa
     9781 ttactaatat catttcaatt attcttcagc aagcccagga ttatgatgtg gcgaaaataa
     9841 catgatggtg aggagaagca gttaaggaaa tccttataac taactgtaat ttgagaagaa
     9901 tatttttacc tattcctttt gcttttgata actgcgcggg aacaaagtaa aaataatgac
     9961 gaagtaaacg gagcatggca gctccgtttc atttgaaagg aattaattaa gcaggccggg
    10021 gaaaatcccc ttaataccag taacgataaa ttcaatcccc aatgccatca gcaatagccc
    10081 cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa acccgtacca gccacggtgc
    10141 catgcggaac aatccccagc aacataaagc gaacaatgca atagccacaa agaaaccaaa
    10201 cagatagcta atgctgtgat aacgcgtacc ccagacgatg gtagaactga tcgcccctgg
    10261 ccccgccatc aacggcaacg ccaatggcac cacaccaatg ctttcacgta ccgcggtttc
    10321 tgatttttct tgcttgttct gtttatcctc gccaagcttg ccgctgatca tcgacatcgc
    10381 tatcgtcacc accaggatgc cccctgcgat acggaacgaa tcaattgata taccaaaaag
    10441 ttgcagaatc gtgtcgccga gaaaaagcga gatccacaag ataatggcca cagacaggtt
    10501 ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga taactggtca tgctgataaa
    10561 gacgggaata atccctaccg ggttgaccag cgcaaataac ccgatgaaaa atttgaagta
    10621 aacgggaaaa tcaaaaaagg tctgaatcac ggttagctcc gaagcaaaag ccggataatg
    10681 ttagccataa attaaggttg aaaagacgcg ctgacaatac gccttttgac agcatttttc
    10741 acctcctaac tacttaaaat tgctatcatt cgttattgtt atctagttgt gcaaaacatg
    10801 ctaatgtagc caccaaatca tactacaatt tattaactgt tagctataat ggcgaaaagc
    10861 gatgctgaaa ggtgtcagct tagcaaaaat ttgatttgga tcacgtaatc agtacccaga
    10921 agtgagtaat cttgcttacg ccacctagaa gtgacgcatt agagataata actctaatgt
    10981 ttaaactctt ttagtaaatc acagtgagtg tgagcgcgag taagcttttg attttcatag
    11041 gttaagcaaa tcatcaccgc actgactata ctctcgtatt cgagcagatg atttactaaa
    11101 aaagtttaac attatcagga gagcattatg gctgttacta atgtcgctga acttaacgca
    11161 ctcgtagagc gtgtaaaaaa agcccagcgt gaatatgcca gtttcactca agagcaagta
    11221 gacaaaatct tccgcgccgc cgctctggct gctgcagatg ctcgaatccc actcgcgaaa
    11281 atggccgttg ccgaatccgg catgggtatc gtcgaagata aagtgatcaa aaaccacttt
    11341 gcttctgaat atatctacaa cgcctataaa gatgaaaaaa cctgtggtgt tctgtctgaa
    11401 gacgacactt ttggtaccat cactatcgct gaaccaatcg gtattatttg cggtatcgtt
    11461 ccgaccacta acccgacttc aactgctatc ttcaaatcgc tgatcagtct gaagacccgt
    11521 aacgccatta tcttctcccc gcacccgcgt gcaaaagatg ccaccaacaa agcggctgat
    11581 atcgttctgc aggctgctat cgctgccggt gctccgaaag atctgatcgg ctggatcgat
    11641 caaccttctg ttgaactgtc taacgcactg atgcaccacc cagacatcaa cctgatcctc
    11701 gcgactggtg gtccgggcat ggttaaagcc gcatacagct ccggtaaacc agctatcggt
    11761 gtaggcgcgg gcaacactcc agttgttatc gatgaaactg ctgatatcaa acgtgcagtt
    11821 gcatctgtac tgatgtccaa aaccttcgac aacggcgtaa tctgtgcttc tgaacagtct
    11881 gttgttgttg ttgactctgt ttatgacgct gtacgtgaac gttttgcaac ccacggcggc
    11941 tatctgttgc agggtaaaga gctgaaagct gttcaggatg ttatcctgaa aaacggtgcg
    12001 ctgaacgcgg ctatcgttgg tcagccagcc tataaaattg ctgaactggc aggcttctct
    12061 gtaccagaaa acaccaagat tctgatcggt gaagtgaccg ttgttgatga aagcgaaccg
    12121 ttcgcacatg aaaaactgtc cccgactctg gcaatgtacc gcgctaaaga tttcgaagac
    12181 gcggtagaaa aagcagagaa actggttgct atgggcggta tcggtcatac ctcttgcctg
    12241 tacactgacc aggataacca accggctcgc gtttcttact tcggtcagaa aatgaaaacg
    12301 gctcgtatcc tgattaacac cccagcgtct cagggtggta tcggtgacct gtataacttc
    12361 aaactcgcac cttccctgac tctgggttgt ggttcctggg ggggtaactc catctctgaa
    12421 aacgttggtc cgaaacacct gatcaacaag aaaaccgttg ctaagcgagc tgaaaacatg
    12481 ttgtggcaca aacttccgaa atctatctac ttccgccgtg gctccctgcc aatcgcgctg
    12541 gatgaagtga ttactgatgg ccacaaacgt gcgctcatcg tgactgaccg cttcctgttc
    12601 aacaatggtt atgctgatca gatcacttcc gtactgaaag cagcaggcgt tgaaactgaa
    12661 gtcttcttcg aagtagaagc ggacccgacc ctgagcatcg ttcgtaaagg tgcagaactg
    12721 gcaaactcct tcaaaccaga cgtgattatc gcgctgggtg gtggttcccc gatggacgcc
    12781 gcgaagatta tgtgggttat gtacgaacat ccggaaactc acttcgaaga gctggcgctg
    12841 cgctttatgg atatccgtaa acgtatctac aagttcccga aaatgggcgt gaaagcgaaa
    12901 atgatcgctg tcaccaccac ttctggtaca ggttctgaag tcactccgtt tgcggttgta
    12961 actgacgacg ctactggtca gaaatatccg ctggcagact atgcgctgac tccggatatg
    13021 gcgattgtcg acgccaacct ggttatggac atgccgaagt ccctgtgtgc tttcggtggt
    13081 ctggacgcag taactcacgc catggaagct tatgtttctg tactggcatc tgagttctct
    13141 gatggtcagg ctctgcaggc actgaaactg ctgaaagaat atctgccagc gtcctaccac
    13201 gaagggtcta aaaatccggt agcgcgtgaa cgtgttcaca gtgcagcaac tatcgcgggt
    13261 atcgcatttg cgaacgcctt cctgggtgta tgtcactcaa tggcgcacaa actgggttcc
    13321 cagttccata ttccgcacgg tctggcaaac gccctgctga tttgtaacgt tattcgctac
    13381 aatgcgaacg acaacccgac caagcagact gcattcagcc agtatgaccg tccgcaggct
    13441 cgccgtcgtt atgctgaaat tgccgaccac ttgggtctga gcgcaccggg cgaccgtact
    13501 gctgctaaga tcgagaaact gctggcatgg ctggaaacgc tgaaagctga actgggtatt
    13561 ccgaaatcta tccgtgaagc tggcgttcag gaagcagact tcctggcgaa cgtggataaa
    13621 ctgtctgaag atgcattcga tgaccagtgc accggcgcta acccgcgtta cccgctgatc
    13681 tccgagctga aacagatcct gctggatacc tactacggtc gtgattatgt agaaggtgaa
    13741 actgcagcga aaaaagaagc cgctccggct aaagctgaga aaaaagcgaa aaaatccgct
    13801 taatcagtag cgctgtctgg caatataaac ggccccttct
//
LOCUS       DAZSBF010000103        13807 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZSBF010000103 DAZSBF010000000
VERSION     DAZSBF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13807
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.104"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHXWD_002399"
     CDS             complement(2..760)
                     /gene="dsbG"
                     /locus_tag="ACHXWD_002399"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFR7621298.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHXWD_002400"
     CDS             1132..1695
                     /gene="ahpC"
                     /locus_tag="ACHXWD_002400"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR7621299.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHXWD_002401"
     CDS             1866..3431
                     /gene="ahpF"
                     /locus_tag="ACHXWD_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR7621300.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHXWD_002402"
     CDS             complement(3552..3980)
                     /gene="uspG"
                     /locus_tag="ACHXWD_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR7621301.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4201..5439
                     /locus_tag="ACHXWD_002403"
                     /pseudo
     CDS             4201..5439
                     /locus_tag="ACHXWD_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHXWD_002404"
     CDS             complement(5443..5532)
                     /gene="yldA"
                     /locus_tag="ACHXWD_002404"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR7621302.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHXWD_002405"
     CDS             complement(5670..6080)
                     /gene="rnk"
                     /locus_tag="ACHXWD_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR7621303.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHXWD_002406"
     CDS             complement(6310..7116)
                     /gene="rna"
                     /locus_tag="ACHXWD_002406"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR7621304.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHXWD_002407"
     CDS             complement(7230..8693)
                     /gene="citT"
                     /locus_tag="ACHXWD_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR7621305.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHXWD_002408"
     CDS             complement(8744..9622)
                     /gene="citG"
                     /locus_tag="ACHXWD_002408"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR7621306.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHXWD_002409"
     CDS             complement(9597..10148)
                     /gene="citX"
                     /locus_tag="ACHXWD_002409"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR7621307.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHXWD_002410"
                     /pseudo
     CDS             complement(10152..11683)
                     /gene="citF"
                     /locus_tag="ACHXWD_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
     gene            complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHXWD_002411"
     CDS             complement(11694..12602)
                     /gene="citE"
                     /locus_tag="ACHXWD_002411"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR7621308.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHXWD_002412"
     CDS             complement(12599..12895)
                     /gene="citD"
                     /locus_tag="ACHXWD_002412"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR7621309.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHXWD_002413"
     CDS             complement(12910..>13807)
                     /gene="citC"
                     /locus_tag="ACHXWD_002413"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR7621310.1"
                     /translation="AGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTK
                     TEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANP
                     FTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEY
                     IISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYN
                     QDMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTL
                     HYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3390 a         3497 c         3673 g         3247 t
ORIGIN      
        1 atcaataagt tggagtcatt acccccataa tgatattaag cgttttctga tcgggcaacc
       61 ccacggcctg ttgcagcgta ttttccttac tcatgtaata gatagccggc gtgacatttg
      121 cccccagatc gtccatcagt ttctcattgt cacttaacac tttcatttgc tctgtactta
      181 cgtttgcagg cacgtttagc ttaagcttgc caccagaggc ttcatattgt tgccaggttt
      241 ttgcgggatc tttggaggca agaattgccg ctgctgtcgc cgggctttct ggcttgataa
      301 ccccaaccaa caatgttctt aattgcactt tgccagaatc tacccacggg cgcgcctgct
      361 gccagaactg tttacaatat gggcagaacg gatcggcgaa gacgtagaca atgaccggcg
      421 catctttttt accgtcgagg atccagtggg attgttccat ccgttgccac atttcgcgtc
      481 cggctggtgc gtaaatttct ttttcgataa gtgtgttact caggttttca cctttctcgt
      541 tgtacatgta accagagata gcgtgcttac catcgggagt caggtagatg gtgacgccca
      601 tatcctgata ctttccgaga taacctttca ttcctccggg ggcatcgaac gttttgatga
      661 ttgtaatgcc ctgtttttca atcgctttta ctggggaagg aagttcctct gcgaaggcga
      721 ttgcaggaag cagagccagt aaaagtatct tttttaacat taatttgtcc ttttcagtca
      781 gtgcaaaagt cgagtaaaag gcataaccta tcactgtcat aggtaagagc ttagatcagg
      841 tgattgccct ttgtttatga gggtgttgta atccatgtcg ttgttgcatt tgtaagggca
      901 acacctcagc ctgcaggcag gcgctgaaga taccaaaggg tagttcagat tacacggtca
      961 cctggaaagg gggccatttt actttttatc gccgctggcg gtgcaaagtt cacaaagttg
     1021 tcttacgaag gttgtaaggt aaaacttatc gatttgataa tggaaacgca ttagccgaat
     1081 cggcaaaaat tggttacctt acatctcatc gaaaacacgg aggaagtata gatgtccttg
     1141 attaacacca aaattaaacc ttttaaaaac caggcattca aaaacggcga attcatcgaa
     1201 atcaccgaaa aagataccga aggccgctgg agcgtcttct tcttctaccc ggctgacttt
     1261 actttcgtat gcccgaccga actgggtgac gttgctgacc actacgaaga actgcagaaa
     1321 ctgggcgtag acgtatacgc agtatctacc gatactcact tcacccacaa agcatggcac
     1381 agcagctctg aaaccatcgc taaaatcaaa tatgcgatga tcggcgaccc gactggcgcc
     1441 ctgacccgta acttcgacaa catgcgtgaa gatgaaggtc tggctgaccg tgcgaccttc
     1501 gttgttgacc cgcagggtat catccaggca atcgaagtta ccgctgaagg cattggccgt
     1561 gacgcgtctg atctgctgcg taaaatcaaa gcagctcagt acgtagcttc tcacccaggt
     1621 gaagtttgcc cggctaaatg gaaagaaggt gaagcaactc tggctccgtc tctggacctg
     1681 gttggtaaaa tctaaatttc cttcgtcttt cgcgctacca gctccggcgc gaaatacttc
     1741 ggaaattcac ctaattcttc gggtgctgcg gcacccgatt ttcttccccg caccatgatg
     1801 caagctgcat ccaggtagcc gtagaggccg cttgcatgat gatgtttaaa gcccaggaga
     1861 taaacatgct cgacacaaat atgaaaactc aactcaaggc ttaccttgag aaattgacca
     1921 agcctgttga gttaattgcc acgctggatg acagcgctaa atcggcagaa atcaaggaac
     1981 tgttggctga aatcgcagaa ctgtcagaca aagtcacctt taaagaagat aacagcttgc
     2041 cggtgcgtaa gccgtctttc ctgatcacca acccaggttc caaccagggg ccacgttttg
     2101 caggctcccc gctgggccac gagttcacct cgctggtact ggcgttgctg tggaccggtg
     2161 gtcatccgtc gaaagaagcg cagtctctgc tggagcagat tcgccatatt gacggtgatt
     2221 ttgaattcga aacctattac tcgctctctt gccataactg cccggacgtg gtgcaggcgc
     2281 tgaacctgat gagcgtactg aacccgcgca tcaagcacac tgcaattgac ggcggcacct
     2341 tccagaacga aatcaccgat cgcaacgtga tgggcgttcc ggcagtgttc gtaaacggga
     2401 aagagtttgg tcagggccgc atgacgttga ctgaaatcgt tgccaaaatt gatacgggcg
     2461 cggaaaaacg tgcggcagaa gagctgaaca agcgtgatgc ttatgacgta ttaatcgtcg
     2521 gttccggccc ggcgggtgca gcggcagcaa tttactccgc acgtaaaggc atccgtaccg
     2581 gtctgatggg cgaacgtttt ggtggtcaga tcctcgatac cgttgatatc gaaaactaca
     2641 tttctgtacc gaagactgaa gggcagaagc tggcaggcgc actgaaagtt cacgttgatg
     2701 aatacgacgt tgatgtgatc gacagccaga gcgccagcaa actgatccca gcagcagttg
     2761 aaggtggtct gcatcagatt gaaacagctt ctggcgcggt actgaaagca cgcagcatta
     2821 tcgtggcgac cggtgcaaaa tggcgcaaca tgaacgttcc gggcgaagat cagtatcgca
     2881 ccaaaggcgt gacctactgc ccgcactgcg acggcccgct gtttaaaggt aaacgcgtag
     2941 cggttatcgg cggcggtaac tccggcgtgg aagcggcaat tgacctggcg ggtatcgttg
     3001 agcacgtaac gctgctggaa tttgcgccag aaatgaaagc cgaccaggtt ctgcaggaca
     3061 aactgcgcag cctgaaaaac gtcgacatta ttctgaatgc gcaaaccacg gaagtgaaag
     3121 gcgacggcag caaagtcgtt ggtctggaat atcgagatcg tgtcagcggc gatattcaca
     3181 acatcgaact ggccggtatt ttcgtgcaga ttggtctgct gccgaacacc aactggctcg
     3241 aaggcgcagt cgaacgtaac cgcatgggtg agattatcat tgatgcgaaa tgcgaaacca
     3301 acgtcaaagg cgtattcgca gcgggtgact gtacgacggt tccgtacaag cagatcatca
     3361 tcgccactgg cgaaggtgcc aaagcctctt tgagtgcttt tgactacctg attcgcacca
     3421 aaactgcata agaagaagta agattcacct gcaattgctt agccgccggg gtcaaacctg
     3481 gcggcttttt tatggcatta aaaagccctg ccgggatgtt ccaggcaggg cggcataaat
     3541 acttttatct gttagcgaat gaccagtaca ggcagattgg cgtggcggat cacgctcgag
     3601 gcgttagaac ctaacagatg ggtcgaaatc gatgggttgc gagaaccgat aactacaaca
     3661 tcagccccca gttcttctgc caactcattg acttcatccc gcacgctacc aaaacggaca
     3721 tgttgtttaa tgcgggaagg atcgatggtg aagtggctga ccatcgtttg cagacgttct
     3781 tctgcttcat gttgcagatg ctcttcaaaa cgacgcacat cagcggcaaa acggtgcagg
     3841 ctcaggctgg ctgacccggg tagtacgtga agtagatgaa taactccgtc atcctgggcg
     3901 aggaattcag cgtggcgaac agctttgtcg ctcaattcca tttcaaatac atcaactggc
     3961 ataatgattg tcttatacat aaccctttct ccctgttaat catgaacaaa tcattcgcca
     4021 tgattataat atttatccct gatatttgtc tggttctttt tccttacgaa cggtttctgt
     4081 gattaatata ttctcactga acaccaggaa ttctcccaaa acctgcggta ccgcccgttt
     4141 tcccgctgtg atagctaccc ttaaagactg actctttttt gaactgtctc tggaggttgc
     4201 atgaaagcat tgacttatca cggcccacat cacgttcagg tagaaaatgt tcccgatccg
     4261 ggcattgaac aggctgatga tattattctg cgtattacgg caacggcgat ctgtggctct
     4321 gacctccatc tttatcgagg caaaataccc taggttaaac atggcgatat tttgggtcat
     4381 gaatttatgg gggaagtcgt tgaaaccgga aaggacgtaa aaaatttgca aaaaggcgac
     4441 cgggtggtaa ttccgtttgt cattgcttgt ggcgactgtt ttttctgtcg aatgcagcaa
     4501 tatgccgcct gcgaaaatac caatgcgggt aaaggcgctg cgctcaataa aaaacagata
     4561 ccagctcccg cggcattgtt tggttatagt cacctgtatg gtggcgttcc tggtggacag
     4621 gcggaatatg tccgcgtccc taaagggaat gtggggccgt ttaaagtacc gcctttgctt
     4681 tcagatgata aagccctttt cctttctgat attctgccaa cggcatggca ggcagcaaaa
     4741 aatgcgcaga tccaacaagg ttcaagcgtt gcagtctatg gtgccggtcc agtgggattg
     4801 ttgacaatcg cctgtgcacg gttgctcggt gcggaacaga tttttgttgt tgatcatcat
     4861 ccctaccgct tgcgtttcgc cgctgaccgc tacggcgcga tcccgattaa tttcgatgaa
     4921 gatagcgatc cggcacagtc aattattgaa caaacggcag gtcaccgggg cgtggatgca
     4981 gtaatagacg ccgtcggttt tgaagcgaaa ggcagcacca cggaaacggt gctgactaac
     5041 ctgaaactgg agggcagcag cggtaaagcg ttgcgccagt gtattgcggc ggtcaggcgt
     5101 ggcggcattg ttagcgtacc gggcgtctac gctggattta ttcacggttt cctgtttggc
     5161 gacgcctttg ataaagggtt gacgtttaaa atgggacaga cccacgttca cgcatggctg
     5221 ggtgaattat taccgttaat tgagaaagga ttactgaaac cagaagaaat tgttacccac
     5281 tatatgccgt ttgaagaggc cgcccgggga tatgagattt tcgaaaaacg tgaagaggag
     5341 tgccgtaagg tgattctggt acccggtgca caaagcgcag aggcggcgca gaaggcggtt
     5401 tcaggtctgg tgaatgcgat gccgggggga acaatatgat cgtcaggagt ggttttcgag
     5461 gtaaaggaca gccatgacga taatcgccgc cataatcaga aatcctatca ggatgtaaaa
     5521 tgcttctgcc atggttattc ccacaaacga aacggaataa ttttgcagca aaagtgaaca
     5581 gtgagaacca ggaaaaattg ctgattttgc gtaaagagga tgcgagtgca tcctctgggc
     5641 aaagcgagtt atcgcttgtg cagatgggat taaagcaggt agtcgccagc agcttctggc
     5701 tggtattcga gttccagcac ttcaaggtgg gttgcaacgc cgcccggaag ttcccagtga
     5761 atggaatcgc caacgcgcag ccccagcagt gcggcaccta ccggagccat aacggaaagc
     5821 tgagtattgc tatcggtcat ttttgccgga tacaccagcg tgcgcacacg cacttcgcca
     5881 tcgctaagat tgcggaattt aacccggctg ttcattgtca ccacgtcgtg tggcatctct
     5941 tctggcgaac acatttgggc gcgatccaac tctgcgttta acgcgtcggc gattggcaaa
     6001 ccagcatagg cgggttgctc cagcagaata tcgatgcgtt cggcatccag gtcgttaatg
     6061 atgatagttg gtctggacat ttttactcca tgtcgtcggt actgcgagtg tcgcagataa
     6121 acatacccaa aagaaaaccc tcaccgtcag gcggcgaggg tttaactcac atgatgatac
     6181 tgactgttgc tcactctttg aagtgatttg cgtcacattc agggaattcc tcaatgcagc
     6241 gcattatgta taattcttaa tcgtcacggt ttatggaaga cgaattagcg tgttttgtaa
     6301 atcagatgat taataaccca ctttatcaat cacaaagttt ttgccacagt tgcctgggtg
     6361 tggttgtggc aagaatgagt ttgcagaaag cggagcgttg atggcgtcag ctttgatcga
     6421 gatctgaatt tcagtcaaat acgcagggtt accctggcac gttagcttaa ctgctttcac
     6481 gttctctttt ccccagcttt tggcaaaggc ggcgtcaaag tcacggcggc tcactgtttt
     6541 accgtaatta tccgcaagga attttccggc ttcgctctct ttaatttctt gattcaggcg
     6601 taccatcgta ccgaagtatg cgtccggatc aaaaccaaag caggcaccgt gtttggcata
     6661 ttcgtagcgt tccaggcagg aacgtccgcc agctcctggc atgacttcac ttagtttagc
     6721 ggccgtttcc agtgataacc cggtttccgg cgatgaacac atccggctgg cgtgcgcttc
     6781 tggtagattc gggattgggc gagtagcgca accgaagcgc atccagcggc gttcatcaac
     6841 accacgggca gcaaccgatt taggcaatcc tggccacaga ccatgtacgg tcagaaaatc
     6901 agctttgttg gtcgtttcgg tttgtaggcg acattcatct cgttcgttac gatttcgatc
     6961 gtgttgactc tggcaaaatc cggtttgcca ggagagggcc aggacatagc gatcaaaatc
     7021 gccatactgt tttgcctgca acgctacggc gttggcagaa gagaagggaa gcagagaaac
     7081 cgcgagcaac gcggcgttac gccagaatgc tttcataatg ggtgtggaac tcatacatac
     7141 actgaatact atctattaaa tcataaaaaa gcccgccagg tgggcttacc tggcgggcgt
     7201 gatgatttat tcagcgtttg gcgaacgtat tagttccaca tggcgagaat cggccagcca
     7261 accaacagca acatagagat gtaaatcacc ccgaagattg cgccaagacg ccagtaatct
     7321 tttgatttca catagccaca gccgtaaata atcaccccag gaccggttgc atacggcgtc
     7381 agacagccca tgataccgat agacagcacc agcaggatac acagttgttc cattggtacg
     7441 cccggaatac ctttaccgac ggccagaata accgacagca tggttgcggt gtgcgcagac
     7501 aggctggcaa acaggtagtg tgcaaagtag aacaccagaa ccagtacaat caccgttgcg
     7561 tttggtgaga atccttccag gtgcgtactc atggtaccgg cgaaccagtc aataaagcca
     7621 gaacgagtca ggccgttagc catcacaacc agagttgcca ggttgaccag cgtgttccat
     7681 gcgctgttat agcgggtaat atctttccaa ggcacaacgt gcagggccag cattagcgaa
     7741 actgccagca gaccaactgc agtagcatta atgacttcac tgccaaaaac ccacaaacct
     7801 aagctgagca atacaaggcc aatcagtgtc cactctctgc gtgtcagcgc acccatggtt
     7861 ttcagttcat cacccgccca ggttgccact tcttcactgt gtgtgatttc cggtttgtac
     7921 agcacgtagg aaagccacgg cgcaatgata agcaagataa ccccaaccgg caggaagcag
     7981 aggaaccact gcaaccagct aatctggata ccggcaattt tgctgacgaa ctccagaccc
     8041 agcacgtttg gtgccgcacc ggtgacaaac atggacgaac tcagactggt actaatgacc
     8101 atcatccaca tcaaatagcc gccaatacga cgcgcggacg gatcgttggg gaatgattta
     8161 aacagcggcg gcaggttttt aatgaccgga aaaaccgtac ccccggtacg cgcggtgttg
     8221 gacggtgtaa acggtgccag cagaatgtcg ataatgacaa tcgcataacc caacgtcagc
     8281 gtgcgtttgc ccatgaattt caccaggaaa agggcaatgc gacgacctaa cccggaaact
     8341 tcatacccta atgcaaaaat aaatgcgcca aataccagcc ataccgtggt gctggaaaaa
     8401 ccagccagac cccatttcag cgcctgtttt tgcgcattaa acgctgggtc agctaattct
     8461 ttggcatcaa agagcaggta attactgcca ataacgcaaa tagtaaccgc aataaaactg
     8521 atcgctgttg ccggaattgg ctcgaggatc atgccgacaa tcattgccac aaacacagcg
     8581 aagtaatgcc atgcctgtgg tggcataccg tcggggacag ggataagaaa catgacaccc
     8641 atcaccacca gtggggccaa tagtttccat atattatctt ttgctaaaga catacgggtt
     8701 ctccgaaaat taatatttcc aaatttatca agtgcttaaa taattaaatc tgtgctaaaa
     8761 accaggtaag gatcagcagg tcggcactgc cgcctggact gagatttcgt tcgatacact
     8821 ccctgtcgaa ctgccggaga taatcgagat cggcgggggt tcgaatgccc cctttttgca
     8881 ataatgtttg cgcctcgcgc tgtagccagc gcaggccccc ctcgccaccg cgcgatgcaa
     8941 cgttggtatc gccgttgatc gccatcagta ggagcaaggt atcgagcaat gccagttcag
     9001 gatctaaccc ctgatccagc agagtgaggt aatgcggcaa ggcgtgattg atcaccagtg
     9061 gataacccgc ttcggcttca ccgcgtgcgc cggtaaggcc aagctgctgg tacaaccgtt
     9121 gacctgccgt cagttgtgaa ttattggtac gcagttctcg atcggtcagg ccacggcaga
     9181 aacttgccgc cgtagaacaa acggttgttg gcgttaccgg ttggttgagt tgaagcaaac
     9241 ggccaattgc cgcacatagt agccctaaag aaaaaatgct gcctttatgc gtgtttacgc
     9301 ccgcagtggc gcggaacata tcaccttcgc aagccatacc aattgggcgt aatccgtgga
     9361 gtaccgcttc tggtgctatt tccgcactac aggcaccaaa ttcaatgaaa cggggtagcc
     9421 agccctgaat cgccagcgcg ctgcggtgga aatcttccag cgccatatct ttatgtgcac
     9481 cgcagttaat ccgatccacg aggcctggtt tcggtgacag attgacttca gtcagcatgg
     9541 cgcgccagcc cagcagggcg tactcatcga tcaatgacgt cgcaagcttt gtggttttag
     9601 ttgacgttgc aggcatcgac atcgttcagc agtgcctcca tgcggttgag taaatcggtc
     9661 agttgatggg tttttccacg cgcgcagacg gctgcgcttt gctcgcacaa caggcagcgg
     9721 cgaggcggca gtgaatagtc gcggcgggag agaatttcgc cttcgggcgt caggacatcg
     9781 atatcccata accgcccgag aggatgacta tgttcaagct caatggtggc gagcttgagg
     9841 tcgcgagccg gggcggcaat gctcaacatg ccctccggcc cgctggcgga aaccagtgca
     9901 gcctgctcct gaatttgcca gccctgtttt gcggctaagg cacgcaaggc tgtcacgcca
     9961 tgattaaaaa ttcggcgtgt gacctcgctg tctttaatcg gcccaggcgc aaccacggta
    10021 aaggagacca gtggaacagg atggcgcttg agccagacgt gttgccgtgc ttgcctttca
    10081 tcccggctga cgagcagctc gggaattgac accgcatggt ggctggcgag ttcaggaagc
    10141 aggtgcatgg cttattcctt cacctgatgc acaacatcga tcaccgagcc atcgcggtaa
    10201 cgcacaacgg caacgacgcg gtcggtgaat tcaatcggct gtggttcacc ggtcagcaga
    10261 cgcgcgcgtt cgcgcagcca ctcaatggaa accactttaa tgcccgcttc ctgcagacgt
    10321 tctgccagtt ccggacgtgc cgggttaact gcgataccgt ggtctgtgac cagaatatcg
    10381 acactggagc ctggggtgat gcaggtcagc acgttatcca ccagagtcgg aatacgaccg
    10441 cgtaccagcg gcgcgacgat gatggaaagc gcagaggcaa tcgcggtatc cagtgaccac
    10501 cggaagcacc acgcagtacg ccgtcagagc cggtcagcac gttaacgttg aactgggtgt
    10561 caatttccag cgcgctcagt accaccacgt cgagacgatc aaccgatgcg cctttcgaac
    10621 cccagttagc gtactggttg gcgctgattt cgatgtgatt ggggttacgg gccagcgatt
    10681 gcgcagcatg gctgtcaaag ctctgcacat ccagcagttt gcggatcaga cctttttcgt
    10741 gcagatcaac catcgtcgcg gtaataccgc cgagggcgaa gtcggcgcga atatcgcggc
    10801 tacgcatttt gtcttccagg aaacgggtta ccgccagcga tgcgccgccg gtgccggttt
    10861 gcatggagaa accttctttg aagtagccag agttgacaat cacatccgca gcgctacggg
    10921 caataagcag ttcgcgcggg ttagtggtca tacgggtcgc gccagcgccg atttttgcag
    10981 catcgccaac gcggtcaact ttgacgatca aatcaacctg atcttgctca atgcttgccg
    11041 gattatgcgg ataaggcagc agttcttcgg taagcatcac aacctgtttt gcgttgtcgg
    11101 catcaaccat tgcatagccg agggagccgc agcaggcttt accggtgtaa ccgttggcat
    11161 taccgaattc atcacaggac gggacgccga ggaaagccac gtcgatattc agttcgccgc
    11221 tctgtaccag atgcacacgt ccgccgtgag agtggatttg caccggttcc gcaagcagac
    11281 cacgggaaat ttcttctgcc agtggaccgc gcaggccgga ggtataaatg cgggtaacca
    11341 cgccctggcg aatgtgttct accagtggcg catggcaatc actcagggag ctggacgcca
    11401 gggtcaggtt tttaaagccc atcttagcga tgacgtccat caccatattg acggtcaggt
    11461 caccgccacg gaaagcgtga tggaaggaaa ccgtcatgcc gtcctgtaaa ccagagcgac
    11521 gaatcgcttc ttcctggttg gcgcacagtt tgcgatcgcg cgctttttca gcctggtagg
    11581 tttgctttgg tgagttctgg aaagcggcaa gatcgcattc agcgcgacga ttccaggccg
    11641 ctacccgttc ttgtcgttga gattgttcaa ttttctgcgt cattttgatt gccttattct
    11701 tcgcggatgc cggaaagttc tgcacgggag agcaccagac gggcacgatc gataaccgga
    11761 ccgtccacca tcttgccgtt cagggaaacc acgccgaggc cttcgcgagc ggcggcttca
    11821 gcggcttcta cgacgcggcg ggcgtgatcc acttctttct gggtcggtgc gtagaggttg
    11881 tgcaacagat caatctgacg cgggttgatc agcgatttgc cgtcaaagcc cagctgtttg
    11941 atgtgggcgg cttcttgcag gaatccggct tcgttgttag cgtcggaata gacggtatcg
    12001 aacgcctgaa tacccgcaga gcgcgcggcc tgcaaaatgg aacagcgtgc gaacagcagt
    12061 tcagttcctt ccggggagcg ttctgtacgc aggttgcgca catagtcttc tgcaccgagg
    12121 gcgataccga tcaaacgctc ggaagcgtga gcgatttcca ctgcgcgggt aatccccagc
    12181 ggagattcaa tcgccgccag caggccggtg ctgccgggtt cacgaccaca ggctttttcg
    12241 atacgcagga tctctttttc gatatccaga acatcctgag cggtatcggt tttcggcaga
    12301 cgcacaacgt ccgcaccacc gcgaacgacg gcttccaggt cgttgacacc ccattcggaa
    12361 tccagcgcgt tgacacgcac aatggtttca atatcgcgat acagcggatg ttgcagcgcg
    12421 tggtaaacca tgcggcgggc ggtgtctttt tcacgcaatg ctacggagtc ttcgaggtca
    12481 aacatcaggg catcagccgg gtagatgaag gagttgctga ccatcgcggc attggcacca
    12541 ggcacaaaca acatgctgcg gcgggtgcga gttttacgtt gttgcagcga agcggaaatc
    12601 attggcaatc ctcccatggc agagccggga taccgctggc gcgtgccagc agggcttcca
    12661 gtcgtgcacg taaaatgcag tccagtgcgc ctttgtcatc gacattcagc tgtacgccgc
    12721 gcacgttgta gcgggcgaga acgtccagaa tggtggtgcg aattgcatcg ccaaactgtt
    12781 tctcaacgct gctattgatt tgcaggtcga tatcctgcgt atcgagtggg gcgatgcgta
    12841 tcatcacatc cccagactca agggtgcctg caacggcggg ctggtttatt ttcatttttc
    12901 acctgtttct catgcggggg tcttttgacg agctgccgcg tcctggcggg agtgctcaag
    12961 caggttctgc aaataatgca gcgtgactgc agggaccagc ggcgcgatag ccgtgagatc
    13021 gtttttcgcc agcagttgac gtacccggga agcggatatc ggcatctcct ggtaacgcag
    13081 ccgctcaatt tcaaccagtt cgatgggcgg tgcggagata gtcggcgttt ccagccagta
    13141 gcgcatatcc tggttgtact gggcggtaac gcgacaaaag ggttcagtac cgacaaagcg
    13201 gtgagttaca cccagcgcgg gagcgaggta ctgacggaaa atcttcagat caatttcggt
    13261 gtaacaatgg ttaatgacgc tctgttcttt aatgaagtag caagggaacg tagcgcggga
    13321 gatgatgtat tcggagccgc gatgcacagt caggcgtgga atatcggcgg tgccttttaa
    13381 caccagatcc aaccggtctt cataggggaa gcgtgaagaa tcttctttga ctaaaaacag
    13441 atgcaaccag tcgcactgtg ccgcagcttg ttgaatcaga taacggtgac cattcgtgaa
    13501 gggattggcg ttcatcacaa tgcagccaat cttgttccct ggatgacgaa attttttcag
    13561 cgattcggca tagcgtttca gccgcgtggc gctgttttcc atcagcacca tcacgccggg
    13621 tacgctggtc agcgtggaaa aaccgcactg gcggaacagc gcctcgtatt cggttttggt
    13681 ataaataaac agatgcgtgc tgtgccgctc ataggcgagg tttatcaatt cagtggctaa
    13741 tgtcagcgcc agtccttcac cgcgaacgga ttcactgata gcaacgcatt taataatatt
    13801 tccggca
//
LOCUS       DAZSBF010000104        13794 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZSBF010000104 DAZSBF010000000
VERSION     DAZSBF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.105"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..147)
                     /locus_tag="ACHXWD_002414"
     CDS             complement(<1..147)
                     /locus_tag="ACHXWD_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFR7621311.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="ACHXWD_002415"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="ACHXWD_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="ACHXWD_002416"
                     /pseudo
     CDS             476..1021
                     /locus_tag="ACHXWD_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHXWD_002417"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="ACHXWD_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR7621312.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="ACHXWD_002418"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="ACHXWD_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR7621313.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="ACHXWD_002419"
     CDS             3028..3201
                     /locus_tag="ACHXWD_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621314.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            3227..3295
                     /gene="yncP"
                     /locus_tag="ACHXWD_002420"
     CDS             3227..3295
                     /gene="yncP"
                     /locus_tag="ACHXWD_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR7621315.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHXWD_002421"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="ACHXWD_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR7621316.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="ACHXWD_002422"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="ACHXWD_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR7621317.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="ACHXWD_002423"
     CDS             complement(5499..6422)
                     /locus_tag="ACHXWD_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7621318.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="ACHXWD_002424"
     CDS             6639..7982
                     /locus_tag="ACHXWD_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR7621319.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="ACHXWD_002425"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="ACHXWD_002425"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR7621320.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="ACHXWD_002426"
     CDS             10002..10226
                     /locus_tag="ACHXWD_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR7621321.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="ACHXWD_002427"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="ACHXWD_002427"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR7621322.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHXWD_002428"
     CDS             complement(10820..11800)
                     /gene="ydcK"
                     /locus_tag="ACHXWD_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR7621323.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="ACHXWD_002429"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="ACHXWD_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR7621324.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="ACHXWD_002430"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="ACHXWD_002430"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR7621325.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3483 g         3574 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gcgcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAZSBF010000105        13492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZSBF010000105 DAZSBF010000000
VERSION     DAZSBF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.106"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(36..119)
                     /locus_tag="ACHXWD_002431"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="ACHXWD_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHXWD_002432"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="ACHXWD_002432"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR7621326.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHXWD_002433"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="ACHXWD_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR7621327.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHXWD_002434"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="ACHXWD_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR7621328.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="ACHXWD_002435"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="ACHXWD_002435"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR7621329.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="ACHXWD_002436"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="ACHXWD_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR7621330.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="ACHXWD_002437"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="ACHXWD_002437"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR7621331.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="ACHXWD_002438"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="ACHXWD_002438"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR7621332.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="ACHXWD_002439"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="ACHXWD_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHXWD_002440"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="ACHXWD_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR7621333.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="ACHXWD_002441"
     CDS             complement(12425..12664)
                     /locus_tag="ACHXWD_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR7621334.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHXWD_002442"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="ACHXWD_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR7621335.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13041..13328
                     /locus_tag="ACHXWD_002443"
     CDS             13041..13328
                     /locus_tag="ACHXWD_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR7621336.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
BASE COUNT         3519 a         3319 c         3468 g         3186 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggcgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagattt tcttcctctc cagcagtggt agaatagcgc
    13021 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    13081 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    13141 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    13201 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    13261 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    13321 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    13381 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    13441 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tc
//
LOCUS       DAZSBF010000106        13265 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZSBF010000106 DAZSBF010000000
VERSION     DAZSBF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.107"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(501..1592)
                     /gene="ychF"
                     /locus_tag="ACHXWD_002444"
     CDS             complement(501..1592)
                     /gene="ychF"
                     /locus_tag="ACHXWD_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR7621337.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1709..2293)
                     /gene="pth"
                     /locus_tag="ACHXWD_002445"
     CDS             complement(1709..2293)
                     /gene="pth"
                     /locus_tag="ACHXWD_002445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR7621338.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2570..2848
                     /gene="ychH"
                     /locus_tag="ACHXWD_002446"
     CDS             2570..2848
                     /gene="ychH"
                     /locus_tag="ACHXWD_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR7621339.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2903..4582)
                     /gene="dauA"
                     /locus_tag="ACHXWD_002447"
     CDS             complement(2903..4582)
                     /gene="dauA"
                     /locus_tag="ACHXWD_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR7621340.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4706..5653)
                     /gene="prs"
                     /locus_tag="ACHXWD_002448"
     CDS             complement(4706..5653)
                     /gene="prs"
                     /locus_tag="ACHXWD_002448"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR7621341.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5804..6655)
                     /gene="ispE"
                     /locus_tag="ACHXWD_002449"
     CDS             complement(5804..6655)
                     /gene="ispE"
                     /locus_tag="ACHXWD_002449"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR7621342.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6655..7278)
                     /gene="lolB"
                     /locus_tag="ACHXWD_002450"
     CDS             complement(6655..7278)
                     /gene="lolB"
                     /locus_tag="ACHXWD_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR7621343.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7492..8748
                     /gene="hemA"
                     /locus_tag="ACHXWD_002451"
     CDS             7492..8748
                     /gene="hemA"
                     /locus_tag="ACHXWD_002451"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR7621344.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8790..9872
                     /gene="prfA"
                     /locus_tag="ACHXWD_002452"
     CDS             8790..9872
                     /gene="prfA"
                     /locus_tag="ACHXWD_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR7621345.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9872..10705
                     /gene="prmC"
                     /locus_tag="ACHXWD_002453"
     CDS             9872..10705
                     /gene="prmC"
                     /locus_tag="ACHXWD_002453"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR7621346.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10702..11094
                     /gene="sirB2"
                     /locus_tag="ACHXWD_002454"
     CDS             10702..11094
                     /gene="sirB2"
                     /locus_tag="ACHXWD_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR7621347.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11098..11906
                     /gene="sirB1"
                     /locus_tag="ACHXWD_002455"
                     /pseudo
     CDS             11098..11906
                     /gene="sirB1"
                     /locus_tag="ACHXWD_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
     gene            11942..12796
                     /gene="kdsA"
                     /locus_tag="ACHXWD_002456"
     CDS             11942..12796
                     /gene="kdsA"
                     /locus_tag="ACHXWD_002456"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR7621348.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12945..13052)
                     /locus_tag="ACHXWD_002457"
     CDS             complement(12945..13052)
                     /locus_tag="ACHXWD_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7621349.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3304 a         3339 c         3425 g         3197 t
ORIGIN      
        1 ttgatgtatc tgcataacaa aaacaatatg cgccattttt gcattactaa taagaaaaag
       61 caaaaaacat cgatattcat ttttggtcaa tgggttttct ggcctatcgt tgttattgtg
      121 ctactggtta tcattttcat cttgccagga tcgcacgtaa tcacgttatt cggggacttt
      181 atctgcggga tattaccctt caggtaagtc cttttctcgc cagcatattg gagcgtgatc
      241 aatattgatc agctgtgaag ggggtgatgt gtagttgaac aaaaacttac gttatatcac
      301 tactaatgag atatccgtca tctgacactg atgtctttaa ttattctcgt ggttcactgt
      361 aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg tgaaatgaaa
      421 aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat ttagcgaaac
      481 ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc gccatcttta
      541 acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc ttgttcacct
      601 ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc tttttcaaaa
      661 tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat ggtccatgca
      721 cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt ataaccgaca
      781 cggatcacac ggttcaggcc cggttcttcc agcccaagct cctgcataaa ctcgtcacgt
      841 tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg aaccacaaca
      901 gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt ttcaaaaccg
      961 tcttcgttga cgttggcgat gtacattgtt ggtttcagcg tcaggaagct caggtaacga
     1021 atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc gttttccagc
     1081 tggggcaggc atttttctag gaccgccagc tcagctttcg cgtctttatc gccacctttg
     1141 gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc cagcgccagt
     1201 tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac gtgaatgatg
     1261 ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg gatgttggtc
     1321 aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc ggcgatatcg
     1381 acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc cagttgatcc
     1441 aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca gaatggaaag
     1501 ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt cccgacgttg
     1561 ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat atcttaataa
     1621 tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat tatacacggc
     1681 actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc aatcggttcg
     1741 ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct tcgtcgatgg
     1801 cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa ccgacaactt
     1861 tatttttatc gccaggatga ccgattccga tgcgtaaacg gtgaaagtta gggttattac
     1921 ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg cccaatttaa
     1981 atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt tcgtccggat
     2041 taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc ataaatgtag
     2101 tcgggactaa caggcggaca tcttcgcctc caagagtgac tcgcgaagta taaccaaaga
     2161 atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca acgaaccagg
     2221 caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg ccgacaatca
     2281 atttaatcgt cacgtttttt gtcctgagtg tgtacataac tggcgcgtag tttactggtt
     2341 gcggccccgc ttgacaaaaa actgcgtatc aaatgcagat aacgtaataa ttgcctgagt
     2401 ggactattag aaagtcaagg tgttcaggcg tttatttgta aagttttgtt gaaataaggg
     2461 ttgtaattgt gatcacgccc gcacataacc cacagggtgt tgtctatact ttacacataa
     2521 ggaagagggg tattccctgt tacaacccag aaagttccgg aggtgacata tgaaacgcaa
     2581 aaacgcttcg ttactcggta acgtgctcat ggggttgggg ctggtggtaa tggtggtcgg
     2641 cgtggggtat tcaatcctca accagttacc acagtttaat atgccccagt atttcgcaca
     2701 tggtgcagtg ctaagtattt tcgtcggtgc cattctctgg ctggcgggtg cccgtgttgg
     2761 cgggcatgaa caggtgtgcg accgttactg gtgggttcgt cactatgaca aacgttgccg
     2821 ccgtagcgat aatcgccgtc atagctaaca aataatgcag tttgctgacc agtcagtttg
     2881 cgctgactgg tcaatctcgt acttataaat ccgccatcgc cgcgcgacga ttcgggaaga
     2941 acgcgaggcg tcccgggatc ggttgaatgc ctgcgcgagc catagtgcgc agtggctgga
     3001 attccacgtt gcacacgcgc agttcacatc cttcgggcag acgcttcaca aaacgctgga
     3061 acgcatcaag accaccagca tcaagtaccg gaacggcatc ccacttcaga atcacaatcc
     3121 gtttgccttc aagacgtgac tccaggtccg tgaacaagcc ttcagcagca gcaaaaaaca
     3181 gcgggccaat aacgcgcagt accagaacat cgtctggaac atctacgact accggtgcca
     3241 ggcgagtcat acgtgcgata cgacgcataa acagcagcga tgccagcacg atccccacgc
     3301 tgatggcaat aaccatatca aacagcacgg tcagcgacat gcacagcagc atgacaatga
     3361 tgtcatcttt cggtgcatga cgcagcaagt cgaccacttt atgcgcttca ctcatgttcc
     3421 acgccaccat caacagcagg gctgccatag cggaaagcgg cagccaggag agcagcggtg
     3481 ccagtaccag cagggcaaga ataaccagaa tagagtggat caccgccgag ataggggaag
     3541 ttgccccggc acggacgtta gcggcagaac gcgcgatggc agctgtagcg gtaataccac
     3601 caaagaacgg agcgataata ttccccagtc cctgtccaac cagttcgctg ttcgccttgt
     3661 gtttcgtccc ggtcatacca tccagtacca cggcgcagag cagagattcg attgcgccga
     3721 gcattgccat tgagaatgcc gcaggcagca gtgtgcgaat agaatcccag gttagcgtga
     3781 attctgaatt aggcagatcc cacggcagca ccagttgcgg cagcagttgc ggaataccgt
     3841 taccctgaga accatcggcc aggacgtagt ggaattgcga accgatggta gcaacatgtc
     3901 cgccgagcag gttaacaatc cccatcaccg cgcaaccagc cagcaaggcc ggaaggtgac
     3961 cgggtaaacg aatgcccaga cgcggccaaa aaacaagaat acctagcgtc acaatgccaa
     4021 tggcagcatc acccacatta atggtcggca gcgccataaa taatgcgccg actttttgta
     4081 gataatgttc cgggacatgg gccatttgca gaccgagaaa atctttaatc tgcatggtac
     4141 cgatggtgat cccgataccc gaggtgaaac ctaaggtgac ggaaaccgga atatactcaa
     4201 tcaggcgacc aaagcgtgcc agacccatca gaatcaaaaa gatccccgac agcaaggtcg
     4261 caaccagcag tcctgccagt ccaaactgtt gcgacacggg atagagaatt accacaaatg
     4321 ccgcagtcgg accggaaacg ctaaagcgtg acccacccgt cagagcaatg acaatccccg
     4381 caacagctgc ggtatataaa ccgtactggg gtgccacacc actaccaata gccaacgcca
     4441 tcgccagcgg gatagcaata atcccgacgg ttatcccggc aatcaggtca cgggtaaacc
     4501 gtgcggcagt atatttttct ttccagcaag cgtcgatcag agcgcggaaa ggcatcacat
     4561 gtgaggaaaa tattttgttc acaataatgt ttcatccgtg agcgcatcat ctgtcaacta
     4621 aatggcaggt gaaggaggca taggtcatac aaatggatat tacagacaaa aaaacccgcc
     4681 gcagcgggtc tttgagcggg ttcgattagt gttcgaacat ggcagagatc gattcttcgt
     4741 tgctgatacg acgaatcgct tcggccagca tacctgacag ggtcagagta cgcacgttcg
     4801 gcagtgattt gatttcatcg ctcagcggaa tggtatcgca gacaacgact tcatcaatta
     4861 cagagttacg caggttgttc gccgcgttgc cagagaagat cgggtgagtc gcgtacgcaa
     4921 atacacgttt agcaccacgt tctttcagag cttcagcagc tttacacagc gtaccgccag
     4981 tgtcgatcat atcatcgacc agtacgcagt cacgacctgc aacgtcaccg atgatatgca
     5041 tcacctggga aacgttcgca cgcggacgac gtttgtcgat gattgccata tcggtatcgt
     5101 tcagcagctt agcgatagcg cgggcacgca caacgccgcc gatgtccgga gaaaccacaa
     5161 ttgggttatc cagattcagc tgcagcatgt cttccagcag gatcgggcta ccaaatacgt
     5221 tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc gtgcagatcc actgtcagca
     5281 cacggtcaac accgacgctg gagaggaagt ctgcaaccac tttcgcagtg attggtacac
     5341 gagcggaacg gacgcgacgg tcctggcgcg catagccaaa gtaggggata acagcggtga
     5401 tacgacctgc ggaagcacga cgcagggcat caaccataac gactaattcc atcaggttgt
     5461 cgttagtagg ggcacaagtg gactggatga tgaaaatatc accaccgcgt acattttcgt
     5521 tgatttgtac actgacttcg ccgtcgctaa agcgacctac agcggcgtcg ccgagtgaag
     5581 tgtacaggcg gttggcaata cgttgtgcta gttccggggt ggcgttacca gcaaaaagct
     5641 tcatatcagg cacgagaaga acctcaggca tgcgtccatt ggtggaaaga atctgccgaa
     5701 aactgtgcgg gccaggcatg atcctttcca ggcggtgtat taaagagcgc gatgcaacgt
     5761 ctggaacaag gtgacgttgt caccgaaact cagcttgccc ggcttaaagc atggctctgt
     5821 gcaatgggga aagattaaca cctttcgcca caaagccatt gagccattcc ggggcttgct
     5881 ctagcacctg gcgggcttca gactctgtat caaattcagc aaagacacag gcccctgtcc
     5941 cagtcaggcg cgacggggcg tattctaaca gccaggaaag caccgcatca acctcgcgaa
     6001 aacgttttct tgcgataacc tcgcaatcat tgctgaattc acattttagc aacgtttcta
     6061 ttgacctttt tggcgtattg cgcgggagtt caggatcttt aaaaatcact ggtgtcggaa
     6121 tacttacacc agggtgcgcc accagatacc acttctctgg cggatccacc ggcgttagta
     6181 tttcaccaac gccttcggca aacgcggcat gcccccgaac aaagacagga acatctgcgc
     6241 ccagcgtcag ccccatttcc gccagctcat ccatgcttag cccgcattgc cagagatgat
     6301 ttaatgccac caggaccgtc gcggcattgg atgaaccacc gccgagaccg ccgcccatcg
     6361 gcaaacgctt gtcaatgctg atattcgcac cgcttcccgt cggaagacgc ccgctgtctg
     6421 ccgcagtttt catcaacaac cgcgctgcgc gaacgatcag gttatcttca tgttccacgc
     6481 cttcaacggg cgttaacaga cgaatatccc catcgtcacg aagctcaatg ctgatggtgt
     6541 cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg gtaaccatcc gcacgctgac
     6601 cggtaatgta taaaaacaga ttaagttttg ccggagaggg ccactgtgtc cgcattattt
     6661 cactatccag ttatccattt ttaacttgat gcgttgacca ccgtcggtga gttccatatt
     6721 ggctggcatc gcaggttgcg ttttggtgtc ataaccacca taaacaacct tccagttttt
     6781 gccattctgg ctgtaggtaa tttcgctcag gcggtactgg tcgtccagtt tgtagtcggt
     6841 tgcatcaccc ggtaaaccta aaatccattg gcgcaagctg ttgagcggaa ttggcattcc
     6901 ggtcaatttg ccaatcatct cttcggcgtc atcggcggta taacgctgac ctttattgtc
     6961 gactaactgc acgttacccg gttgagcatt cagctccagt tccgtgctgc ccaatgggtt
     7021 agtgagcagc agacggtagc gatcctggcc ggtttgctgc cagaaaaagc gggcgtacac
     7081 tttttgttgg tcagaaatat aagcgaacgc gccgcgagtc tgatactgat taagattgcg
     7141 cacgtcttgc tgatgctgac gccattgtgg cgaatccggg cttttgccag gacctttggg
     7201 cgtggtaacg gaacaggcag tgagcacaag agcagccagc ggtagcaggc ggataagacg
     7261 aaaatcgggc tggggcatag tgatgacaag tccttgagat acgttgcagt tataaccctt
     7321 aatgctagcg ttaccgtccg ctatcgtcta tgttcaagtt gtcttaattg ccagaatcta
     7381 acggctttcg gcaattactc caaaaggggg cgctctcttt tattgatctt acgcatcctg
     7441 tatgatgcaa gcagactaac catatcaacg ttggtattat ttcccgcaga catgaccctt
     7501 ttagcactcg gtatcaacca taaaacggca cctgtatcgc tgcgagaacg tgtatcgttt
     7561 tcgccggata agctcgatca ggcgcttgac agcctgcttg cgcagccgat ggtgcagggc
     7621 ggcgtggtgc tgtcgacgtg caaccgcacg gaactttatc ttagcgttga agagcaggat
     7681 aacctgcaag aggcgttaat ccgctggctt tgcgattatc acaatcttaa tgaagaagat
     7741 ctgcgtaaaa gcctctactg gcatcaggat aacgacgcgg ttagccattt aatgcgtgtt
     7801 gccagcggcc tggattcact ggttcttggg gagccgcaga tcctcggtca ggttaaaaaa
     7861 gcgtttgccg attcgcaaaa aggtcatatg aaggccagcg aactggaacg catgttccag
     7921 aaatctttct ctgtagcgaa acgcgttcgc actgaaacag atatcggtgc cagtgctgtg
     7981 tctgtcgctt ttgcggcttg tacgctggcg cggcagatct ttgaatcgct ctctacggtc
     8041 acagtgttgc tggtaggcgc gggcgaaacc atcgagctgg tagcgcgtca tctgcgcgaa
     8101 cataaagtac agaagatgat tatcgccaac cgcactcgcg aacgtgccca aattctggca
     8161 gatgaagttg gcgcggaagt gattgccctg agtgagatcg acgaacgtct gcgcgaagcc
     8221 gatatcatca tcagttccac cgccagcccg ttaccgatta tcgggaaagg catggtggag
     8281 cgcgcattaa aaagtcgtcg taaccaacca atgctgttgg tggatattgc cgttccgcgc
     8341 gatgttgagc cggaagttgg caaactggcg aatgcttatc tttatagtgt ggacgatctg
     8401 caaagcatca tttcgcacaa cctggcgcag cgtaaagccg cagcggttga ggcggaaact
     8461 attgtcgctc aggaaaccag cgaatttatg gcgtggctgc gagcacaaag cgccagcgaa
     8521 accattcgcg agtatcgcag ccaggcagag cacgttcgcg atgagttaac cgccaaagcg
     8581 ttagcggccc ttgagcaggg cggcgacgcg caagccatta tgcaggatct ggcatggaaa
     8641 ctgactaacc gcttgatcca tgcgccaacg aaatcacttc aacaggccgc ccgtgacggg
     8701 gataacgaac gcctgaatat tctgcgcgac agcctcgggc tggagtagca gtacatcatt
     8761 ttcttttttt acagggtgca tttacgccta tgaagccttc tatcgttgcc aaactggaag
     8821 ccctgcatga acgccatgaa gaagttcagg cgttgctggg tgacgcgcaa actatcgccg
     8881 accaggaacg ttttcgcgca ttatcacgcg aatatgcgca gttaagtgat gtttcgcgct
     8941 gttttaccga ctggcaacag gttcaggaag atatcgaaac cgcacagatg atgctcgatg
     9001 atcctgaaat gcgtgagatg gcgcaggatg aactgcgcga agctaaagaa aaaagcgagc
     9061 aactggaaca gcaattacag gttctgttac tgccaaaaga tcctgatgac gaacgtaacg
     9121 ccttcctcga agtccgagcc ggaaccggcg gcgacgaagc ggcgctgttc gcgggcgatc
     9181 tgttccgtat gtacagccgt tatgccgaag cccgccgctg gcgggtagaa atcatgagcg
     9241 ccagcgaggg tgaacatggt ggttataaag agatcatcgc caaaattagc ggtgatggtg
     9301 tgtatggtcg tctgaaattt gaatccggcg gtcatcgcgt gcagcgtgtt cctgctacgg
     9361 aatcgcaggg tcgtattcat acttctgctt gtaccgttgc ggtaatgcca gaactgcctg
     9421 acgcagaact gccggacatc aacccagcag atttacgcat tgatactttc cgctcgtcag
     9481 gggcgggtgg tcagcacgtt aacaccaccg attcggcaat tcgtattact cacttgccga
     9541 ccgggattgt tgttgaatgt caggacgaac gttcacaaca taaaaacaaa gctaaagcac
     9601 tttctgtact tggtgctcgt atccacgctg cagaaatggc aaaacggcaa caggccgaag
     9661 cgtctacccg tcgtaacctg ctggggagtg gcgatcgcag cgaccgtaac cgtacttaca
     9721 acttcccgca ggggcgcgtt accgatcacc gcatcaacct gacgctctac cgtctggatg
     9781 aagtgatgga aggtaagctg gatatgctga ttgaaccgat tatccaggaa catcaggccg
     9841 accaactggc ggcgttgtcc gagcaggaat aatggaatat caatactggt tacgtgaagc
     9901 aataagccaa cttcaggcga gcgaaagccc acgccgtgac gctgaaatct tattggagta
     9961 tgtcactggc agggggcgga cttttattct ggcctttggt gaaacgcagc tgactgacga
    10021 acaatgtcag caacttgatg cgctactgac acgtcgtcgc gatggtgaac ccattgctca
    10081 tttaaccggg gtgcgagaat tctggtcgtt gccgttattt gtttcgccag cgaccttaat
    10141 tccgcgcccg gatacggagt gtctggtgga gcaggcactg gcgcggttgc ctgaacaacc
    10201 ttgccgtatt ctcgatctcg ggacgggtac cggggcgatt gcgcttgcgc tggctagcga
    10261 gcgcccggac tgcgaaatta tcgctgtaga tcgtatgcct gatgctgtct ccctggcaca
    10321 acgtaatgcc cagcatctgg cgatcaaaaa tatccacatt ctgcaaagcg actggtttag
    10381 cgcgctagcc gggcagcagt ttgcgatgat tgtcagcaat ccgccgtata ttgacgagca
    10441 ggacccacat cttcaacaag gcgatgtccg ctttgagccg ctcactgcgc tggttgcggc
    10501 agacagtgga atggcagaca tcgtgcatat catcgaacag tcgcgtaacg cgctggtatc
    10561 cggcggcttt ctgcttctgg aacatggctg gcagcagggc gaagcggtgc gacaagcatt
    10621 tatcctcgcg gggtatcatg acgtcgaaac ctgccgtgac tatggtgata acgagcgcgt
    10681 aacgctcggc cgctattatc aatgacaagt ttttctacac tgcttagtgt tcatcttatt
    10741 agtatcgcgc tttctgttgg gctattaacc ttacgtttct ggctacgtta tcagaagcat
    10801 ccacaggcat ttgctcgctg gacgcgcatt gtgccgccgg ttgtcgatac ggtgttattg
    10861 ttaagcggca ttgcgttgat ggctaaagcg cacatcctgc cattttccgg gcaggcacag
    10921 tggctgactg aaaagctgtt tggagttatc atttatatcg ttttgggttt tattgcactc
    10981 gattatcgtc gtatgcacag tcagcaggcg cgcattattg ccttcccgct ggcgttggtg
    11041 gtgctgtaca tcatcattaa actcgccacc acaaaagtac cgttactggg gtaagtcatg
    11101 agatcgttag ctgatttcga atttaataaa gcgccattgt gcgaaggcat gatcctggct
    11161 tgcgaagcaa tccgccgcga ttttccctcg caagatgttt acgacgaact ggagcgtctc
    11221 gttagtctgg cgaaggaaga aatcagccag cttctgcctt tagaagagca gctggaaaaa
    11281 ctgatcgcgc tgttttacgg cgactgggga tttaaagcct cacgcggcgt ttatcgtctt
    11341 tccgatgcat tatggctgga ccaggtgtta aagaatcgac aggcagtgcg gtatcattag
    11401 gtgcggtttt attatgggtc gcgaatcgtc tcgatttgcc gctgctgccg gtgattttcc
    11461 ctacgcagct gatattgcgc attgaatgtc cggatggcga aatttggctg attaatcctt
    11521 ttaacggtga atcgttaagc gaacatatgc tggacgtatg gttaaaggga aatatcagcc
    11581 cgtcggcgga actgttttat gaagaccttg atgaagctga taacattgag gtaatccgca
    11641 aattgttgga tacactcaaa gcctcgttga tggaagaaaa tcagatggag ctggcgttac
    11701 gcaccagcga agctttatta caattcaacc cggaagatcc ctatgaaatt cgcgatcgcg
    11761 ggttgattta tgcgcaactg gattgcgaac acgttgcgtt gaacgattta agttatttcg
    11821 ttgaacagtg tccggaagac ccgatcagcg aaatgatccg tgcgcaaata aataacatcg
    11881 cgcataaaca tattgtgctg cattaattaa tcgacatttt actcaagatt aaggcgatcc
    11941 tatgaaacaa aaagtggtta gcattggcga catcaacgta gcaaatgacc tgccgttcgt
    12001 actgtttggt ggtatgaacg tgctggaatc tcgcgatctg gcgatgcgca tttgcgagca
    12061 ctacgtaacc gtgacccaga aactgggtat cccttacgtg ttcaaagcct cttttgacaa
    12121 agccaaccgc tcctccatcc actcttatcg tggaccgggc ctggaagaag ggatgaaaat
    12181 cttccaggag ttgaagcaga cttttggcgt gaaaattatc accgacgttc acgaaccaag
    12241 tcaggcacag cccgttgctg atgtcgtgga tgtgattcag ttgccggcgt ttcttgctcg
    12301 ccagactgac ctggttgaag ccatggcgaa aaccggtgcg gtaattaacg tcaagaaacc
    12361 acagtttgtc agcccgggac agatgggtaa tatcgttgat aaattcaaag aaggcggcaa
    12421 cgaaaaagtg attctttgcg atcgcggtgc taacttcggc tatgacaacc tggttgtcga
    12481 tatgctgggc ttcagcatta tgaagaaagt gtctggtaac tcgccggtga ttttcgacgt
    12541 gacccacgca ctgcaatgcc gcgatccgtt tggcgcagct tccggtggtc gtcgtgctca
    12601 ggtggctgag ctggcacgag ccggtatggc ggtaggtctg gcggggctgt ttattgaagc
    12661 gcatccggat ccggaacatg cgaaatgtga tggtccatcc gcgctgccgc tggctaaact
    12721 ggaaccgttc ctcaagcaga tgaaagcgat tgatgatctg gtgaaaggtt tcgaagaact
    12781 ggataccagc aagtaatctt ttttgcttga aaaataaagc cttagcgttc tgcgttaaga
    12841 cttttttcat gggtgccaga tacaaaaaag gccgcaggct gttacccctg cggccggttt
    12901 cgggcgcata ttgccatcac ggcagcctga cgcccgtttt caccttactt ccggttacgc
    12961 caccagccga caatcgctgc ggtaataatt cccgccagga tcggtgctgc caggtcgtgc
    13021 cagaaaatca tggcaaactg cgcgagcgtc atatagccgc cttgttgtaa tgacaacatt
    13081 ttgcggctat tcttgaattg ttctggttca agattagccc ccgttctgtt gtcaggttaa
    13141 acctctcaac gtgcgggggt tttctctttc cagcaaccaa tgccaccagg gataaagccc
    13201 ccgcaacatt gcgcctcacc ggataacgcc ggcttgatgt ggatactacg tctcaattca
    13261 tcttc
//
LOCUS       DAZSBF010000107        13262 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZSBF010000107 DAZSBF010000000
VERSION     DAZSBF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.108"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHXWD_002458"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHXWD_002458"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR7621350.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHXWD_002459"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHXWD_002459"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR7621351.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHXWD_002460"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHXWD_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR7621352.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHXWD_002461"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHXWD_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR7621353.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHXWD_002462"
     CDS             complement(4036..5568)
                     /locus_tag="ACHXWD_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR7621354.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACHGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHXWD_002463"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHXWD_002463"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR7621355.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHXWD_002464"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHXWD_002464"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR7621356.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHXWD_002465"
     CDS             complement(8663..10399)
                     /locus_tag="ACHXWD_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR7621357.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHXWD_002466"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHXWD_002466"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR7621358.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHXWD_002467"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHXWD_002467"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR7621359.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHXWD_002468"
     CDS             complement(12121..12855)
                     /locus_tag="ACHXWD_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR7621360.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13262
                     /locus_tag="ACHXWD_002469"
     CDS             13056..>13262
                     /locus_tag="ACHXWD_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR7621361.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3365 a         3370 c         3490 g         3037 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatggcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc tgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 tg
//
LOCUS       DAZSBF010000108        13090 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZSBF010000108 DAZSBF010000000
VERSION     DAZSBF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.109"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            119..418
                     /locus_tag="ACHXWD_002470"
     CDS             119..418
                     /locus_tag="ACHXWD_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621362.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     ILNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(465..1352)
                     /gene="rarD"
                     /locus_tag="ACHXWD_002471"
     CDS             complement(465..1352)
                     /gene="rarD"
                     /locus_tag="ACHXWD_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR7621363.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1404..1871)
                     /gene="yigI"
                     /locus_tag="ACHXWD_002472"
     CDS             complement(1404..1871)
                     /gene="yigI"
                     /locus_tag="ACHXWD_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR7621364.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2036..2905
                     /gene="pldA"
                     /locus_tag="ACHXWD_002473"
     CDS             2036..2905
                     /gene="pldA"
                     /locus_tag="ACHXWD_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR7621365.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3038..4867
                     /gene="recQ"
                     /locus_tag="ACHXWD_002474"
     CDS             3038..4867
                     /gene="recQ"
                     /locus_tag="ACHXWD_002474"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR7621366.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4931..5548
                     /gene="rhtC"
                     /locus_tag="ACHXWD_002475"
     CDS             4931..5548
                     /gene="rhtC"
                     /locus_tag="ACHXWD_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR7621367.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5610..6230)
                     /gene="rhtB"
                     /locus_tag="ACHXWD_002476"
     CDS             complement(5610..6230)
                     /gene="rhtB"
                     /locus_tag="ACHXWD_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR7621368.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6341..7363
                     /gene="pldB"
                     /locus_tag="ACHXWD_002477"
     CDS             6341..7363
                     /gene="pldB"
                     /locus_tag="ACHXWD_002477"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR7621369.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7371..8171
                     /gene="yigL"
                     /locus_tag="ACHXWD_002478"
     CDS             7371..8171
                     /gene="yigL"
                     /locus_tag="ACHXWD_002478"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR7621370.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8247..9146
                     /gene="bioP"
                     /locus_tag="ACHXWD_002479"
     CDS             8247..9146
                     /gene="bioP"
                     /locus_tag="ACHXWD_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR7621371.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9034..9987)
                     /gene="metR"
                     /locus_tag="ACHXWD_002480"
     CDS             complement(9034..9987)
                     /gene="metR"
                     /locus_tag="ACHXWD_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR7621372.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10223..12484
                     /gene="metE"
                     /locus_tag="ACHXWD_002481"
     CDS             10223..12484
                     /gene="metE"
                     /locus_tag="ACHXWD_002481"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR7621373.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12606..12728)
                     /locus_tag="ACHXWD_002482"
     CDS             complement(12606..12728)
                     /locus_tag="ACHXWD_002482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621374.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12769..13014)
                     /locus_tag="ACHXWD_002483"
     CDS             complement(12769..13014)
                     /locus_tag="ACHXWD_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR7621375.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3098 a         3250 c         3489 g         3253 t
ORIGIN      
        1 taactataca gtagtaattt agagtgtgta agtttttatt gatggggtat attttaatta
       61 aatataattg aaatgtattt ttattctata aacaatatct tgggtgaatg gattctatat
      121 gggaaatggt aacttttggc agttgttctt gggtaaagaa ccttacagca aaaaatctga
      181 ttttgttaag gcatggttat tgtggtgggc tattaccatc cttgttctat ttattttaag
      241 cctgactacg atattaaatt taagaagtat aggtataggg ttaagttttg ggttttgtca
      301 aatccttggt agatatacga tgtctctagg atggagtaag tactggggag ttttgggggt
      361 atttcctgta acaaatattg ttctttttct tatcttaatt tttttcaaaa aaagataaat
      421 aagtcagtgg gtaggctctt taaacggcct acccatgtta attcttaact ttttctacgc
      481 tgcgtataaa tcgcatccat cacaaaaatt gccagcgcca cccaaataaa ggcgaaagtc
      541 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
      601 gtcgggccaa tgtactggaa aaagcctaac gttgagagac gcaagcgcgt ggcagcggcg
      661 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
      721 gacatcgggt tttgccccat atggctggtt gagctgtcgg caatagcaaa caggtaaatt
      781 gccgccacgg gcagcagcca catggtttcg attaacatgc cggtttgcgc ttcaacggca
      841 atcttcttgc gtaccagacc gtagaaggca aaactaaatg ccagtcccag cgcgataata
      901 ggtagcgaac caaaagtcca cagctggact aacacgccac atatcgccag aatcaccgcc
      961 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     1021 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     1081 ataaacagta gccagttgcc accaatcagc acggcagaga ctgccagcat aaaaattttc
     1141 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     1201 accataaaga aaaacgacca gatcacgcga tgcgtcagga tttcatcggc gggcacgtag
     1261 taaatcaact tgaagtacgc tggcgctata ccccaaataa aataagcggc aagagcgagt
     1321 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     1381 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     1441 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     1501 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     1561 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     1621 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     1681 atgaccccgc cgtgcaaaat gctttgcgcc cagttgccca ccatcattgg ctgattttta
     1741 aaggccagct gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     1801 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     1861 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     1921 tgttgttggt ttctacttta ggaagggata actaacggct atggagttaa gtatgtaaaa
     1981 tagcccgcag aaaaatattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     2041 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     2101 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     2161 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcattta
     2221 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     2281 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     2341 aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     2401 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     2461 tgccaccgat tactgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     2521 cgactctaac gggcgttccg acccgacctc ccgcagctgg aaccgccttt atactcgcct
     2581 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     2641 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     2701 cctcggtgat gcggtgttga gtgcgaaagg acagtacaac tggaataccg gttacggcgg
     2761 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     2821 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     2881 agttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaacccag
     2941 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     3001 atagcgcctg tttttatttc aggcaatcgg ggggaatgtg gcgcaggcgg aagtgttgaa
     3061 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     3121 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg tcgtcatgcc
     3181 caccggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     3241 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tgcaagccaa
     3301 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gagcagcaac ttgaagtgat
     3361 gacaggctgc cgcaccgggc aaattcgtct gctttatatc gccccggaac gcctgatgct
     3421 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg ttgatgaagc
     3481 gcactgtatc tcccaatggg gccacgattt ccgcccggaa tatgccgcgc ttggtcagtt
     3541 acgccagcgt ttcccgaccc tgccgtttat ggcgctgact gccacagccg acgacaccac
     3601 gcgccaggat atcgtgcgct tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     3661 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     3721 gcgctacgtg caggaacagc gcggtaagtc cggcattatc tactgcaaca gccgggcgaa
     3781 agtagaagac accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc
     3841 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgacctgca
     3901 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     3961 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     4021 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     4081 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatattgagc gccacaaact
     4141 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     4201 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     4261 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     4321 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     4381 cgactatggt catgacaaac tgaaagtcta tggcatgggc cgtgataaaa gccatgaaca
     4441 ttgggtgagc gtgatccgcc agctgattca cctcggcctg gtgacgcaaa atattgccca
     4501 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     4561 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     4621 cggcaactat gatcgtaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     4681 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     4741 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     4801 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     4861 agagtagtca gcagcataaa aaagtgccag gatgaagact ccgtaaacgt ttcccccgcg
     4921 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     4981 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     5041 agcgatgatg ggcgtgctgg gcattacctg cggtgtaatg gtttgggctg ggattgcgtt
     5101 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctta tggttggcgg
     5161 tggcctgtat ctctgctgga tgggttacca gatgctgcgc ggcgcgctga aaaaagaggc
     5221 ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt tcctgaaagg
     5281 tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg tgttctcatt
     5341 gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc tgatcattgt
     5401 cgaaacgctg gcgtggttta tcgtcgttgc cagcctgttt gccctgccgc aaatgcgccg
     5461 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     5521 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     5581 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     5641 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     5701 ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     5761 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     5821 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     5881 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaaggtca
     5941 attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     6001 cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     6061 accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     6121 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     6181 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     6241 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgcta
     6301 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     6361 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     6421 gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     6481 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     6541 cgtaaaatat gcggaactgg cctatgacct gttccatttg gggtttgatg tcttaatcat
     6601 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     6661 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc
     6721 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     6781 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     6841 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     6901 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     6961 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga
     7021 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     7081 tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttgcaggc
     7141 tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc
     7201 cgcgggccat cctgtcgaag gaggacgacc gttggtaatt aaaggtgctt accatgagat
     7261 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccattgttg attttttcaa
     7321 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     7381 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     7441 ccaaagaaac cctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     7501 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gatcaagtcg tacatgatca
     7561 cctccaacgg cgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg
     7621 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatctggac atcattacta
     7681 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt
     7741 ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag ccggaaggcg
     7801 tcagcaaagt gttcttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     7861 ttaacgctcg ttggggcgat cgcgtcaacg ttagtttctc taccttaacc tgtctggaag
     7921 tgatggcggg cggcgtttca aaaggccatg cgctggaagc ggtggcgaag aaactgggct
     7981 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgatgccgaa atgctgtcga
     8041 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac cttcatcccg
     8101 agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
     8161 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
     8221 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
     8281 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
     8341 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
     8401 atagcctgaa aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt
     8461 atatgctgag tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg
     8521 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
     8581 ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc
     8641 aggttaccga ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg
     8701 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg
     8761 cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg
     8821 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
     8881 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
     8941 gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta
     9001 tctggcacca acagccgcac tggccaacgt tcattacagg cgcgctggtg atcctggcct
     9061 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
     9121 ggcgtgattg cgcgctgagc gaataaatgc ttctgtaatc ggctgacgct gctcgccatc
     9181 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
     9241 accttggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
     9301 ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
     9361 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaatcaa
     9421 cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc
     9481 aggtgctaac accagacgca cttcatagtc gaacatcggc gaataatgca ggccactgcg
     9541 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg caggctgcgg
     9601 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt
     9661 ttctaacgcg ggcgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
     9721 cgtctgctgc ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt
     9781 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
     9841 aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
     9901 cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca acgcttgtag
     9961 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
    10021 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc
    10081 cttcgtcttt taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca
    10141 gtaagctccc ggggatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt
    10201 acatataatt agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt
    10261 tggcctgcgt cgcgagctga aaaaagcgca agaaagttat tgggcgggga actccacgcg
    10321 tgaagaactg ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca
    10381 agcgggtatc gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac
    10441 cagtttgctg ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat
    10501 cgacaccctg ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc
    10561 ggaaatgacc aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg
    10621 ccaacagttc aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct
    10681 gggccacaac gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt
    10741 gaaaggtgaa cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca
    10801 agtgctggca gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct
    10861 ggtactggaa ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca
    10921 gggacaggtg aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac
    10981 gattactgcg ctgcctgttc agggtctgca tgttgatctc gtacatggta aagatgacgt
    11041 tgctgaactg cacaagcgcc tgccttctga ctggttgctg tctgcgggtc tgatcaatgg
    11101 tcgtaacgtc tggcgcgccg atcttaccga gaaatatgcg caaattaagg acattgtcgg
    11161 caaacgtgat ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag
    11221 cgtggaaacg cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg
    11281 ccatgaacta gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga
    11341 gtggagcgcc ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga
    11401 aaagcgtctg gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg
    11461 tgctgaagcc cagcgtgcac gttttaaact gccagcgtgg ccgaccacca cgattggttc
    11521 cttcccgcaa accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga
    11581 cgccaataac taccgcacgg gcattgcgga acatatcagg caggccattg ttgaacagga
    11641 acgtttggga ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata
    11701 ctttggcgag cacctcgacg ggtttgtctt tacgcaaaac ggttgggtac agagctacgg
    11761 ttcccgctgc gtgaagccac cgattgttat tggtgacgtt agtcgcccgg caccgattac
    11821 cgtggagtgg gcgaagtatg cgcaatctct gactgataaa ccggtgaaag ggatgctgac
    11881 tggcccggtg actattctct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat
    11941 cgccaaacag attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat
    12001 tggcatcatc cagattgacg aaccggctct gcgcgaaggt ttaccgctgc gtcgtagcga
    12061 ctgggatgcg tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa
    12121 agatgacaca caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc
    12181 gattgcggca ctggacgcag acgtcatcac catcgaaacc tcgcgttctg acatggagtt
    12241 gctggagtcg ttcgaagagt ttgattatcc gaatgaaatc ggtccaggcg tctatgacat
    12301 tcactcgcca aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa
    12361 acgcattccg gcagagcgcc tgtgggttaa cccggactgt ggcctgaaaa cgcgcggctg
    12421 gccagaaacc cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg
    12481 atgatgaata ttaacgagtc actgtaaatt gcagtacgat gaaagaggca aaactgcctg
    12541 atactaggcc tacataattt atgcaattta ttgaattttt gggttttgtt ggccggacaa
    12601 ggcgttcacg ccgcatccgg catgaacaac gcgcactttg ccaataatct gatggcttcc
    12661 atcaccggaa gccattttgc tatctgcgcc cgttcagata cagaaataac cgaatcattt
    12721 ttacgcaaaa tataatcctg agcgatttgt tcagtaatca acgctacgtt atttattctc
    12781 acttttcacg aaattgtatc ccttttcagt aacgtggtag tgaggcatag acttatgtcc
    12841 gctatcctca ataataacca aaccctcgtt agctaatttg ttaacaatgt taacaacatt
    12901 accgaatcct ggaatttgcc ttatggatag tgtgcgatcg agattcatcc agttccagtt
    12961 tatatctttc tcagccataa ttttcagtac gttaatttca gtttcagtta tcatagtcaa
    13021 gtgcatcctg gtggtataag tcattaagtt ctcccttaag tttataatct tctagagtag
    13081 cagtatgcgt
//
LOCUS       DAZSBF010000109        12998 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZSBF010000109 DAZSBF010000000
VERSION     DAZSBF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.110"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHXWD_002484"
     CDS             <1..191
                     /locus_tag="ACHXWD_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621376.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHXWD_002485"
     CDS             191..1393
                     /locus_tag="ACHXWD_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR7621377.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHXWD_002486"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHXWD_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR7621378.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHXWD_002487"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHXWD_002487"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR7621379.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHXWD_002488"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHXWD_002488"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR7621380.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHXWD_002489"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHXWD_002489"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR7621381.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHXWD_002490"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHXWD_002490"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR7621382.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHXWD_002491"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHXWD_002491"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR7621383.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHXWD_002492"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHXWD_002492"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR7621384.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3577 g         3153 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggcgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc atcaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gattgcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctagtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgcgggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZSBF010000110        12918 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZSBF010000110 DAZSBF010000000
VERSION     DAZSBF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.111"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            32..547
                     /locus_tag="ACHXWD_002493"
     CDS             32..547
                     /locus_tag="ACHXWD_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7621385.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            765..1493
                     /gene="artP"
                     /locus_tag="ACHXWD_002494"
     CDS             765..1493
                     /gene="artP"
                     /locus_tag="ACHXWD_002494"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR7621386.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1511..2242
                     /gene="artI"
                     /locus_tag="ACHXWD_002495"
     CDS             1511..2242
                     /gene="artI"
                     /locus_tag="ACHXWD_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR7621387.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2249..2965
                     /gene="artQ"
                     /locus_tag="ACHXWD_002496"
     CDS             2249..2965
                     /gene="artQ"
                     /locus_tag="ACHXWD_002496"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR7621388.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            2965..3633
                     /gene="artM"
                     /locus_tag="ACHXWD_002497"
     CDS             2965..3633
                     /gene="artM"
                     /locus_tag="ACHXWD_002497"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR7621389.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            3925..4656
                     /gene="artJ"
                     /locus_tag="ACHXWD_002498"
     CDS             3925..4656
                     /gene="artJ"
                     /locus_tag="ACHXWD_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR7621390.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(4831..5958)
                     /gene="rlmC"
                     /locus_tag="ACHXWD_002499"
     CDS             complement(4831..5958)
                     /gene="rlmC"
                     /locus_tag="ACHXWD_002499"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR7621391.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(5999..6487)
                     /locus_tag="ACHXWD_002500"
     CDS             complement(5999..6487)
                     /locus_tag="ACHXWD_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR7621392.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6547..7392)
                     /gene="potI"
                     /locus_tag="ACHXWD_002501"
     CDS             complement(6547..7392)
                     /gene="potI"
                     /locus_tag="ACHXWD_002501"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR7621393.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7389..8342)
                     /gene="potH"
                     /locus_tag="ACHXWD_002502"
     CDS             complement(7389..8342)
                     /gene="potH"
                     /locus_tag="ACHXWD_002502"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR7621394.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8352..9485)
                     /gene="potG"
                     /locus_tag="ACHXWD_002503"
     CDS             complement(8352..9485)
                     /gene="potG"
                     /locus_tag="ACHXWD_002503"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR7621395.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(9580..10692)
                     /gene="potF"
                     /locus_tag="ACHXWD_002504"
     CDS             complement(9580..10692)
                     /gene="potF"
                     /locus_tag="ACHXWD_002504"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR7621396.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11043..11519)
                     /locus_tag="ACHXWD_002505"
     CDS             complement(11043..11519)
                     /locus_tag="ACHXWD_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR7621397.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(11607..12509)
                     /gene="rimK"
                     /locus_tag="ACHXWD_002506"
     CDS             complement(11607..12509)
                     /gene="rimK"
                     /locus_tag="ACHXWD_002506"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR7621398.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(12570..>12918)
                     /gene="nfsA"
                     /locus_tag="ACHXWD_002507"
     CDS             complement(12570..>12918)
                     /gene="nfsA"
                     /locus_tag="ACHXWD_002507"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR7621399.1"
                     /translation="GVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPR
                     LPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPF
                     ILDYLHKQGWATR"
BASE COUNT         3246 a         3469 c         3203 g         3000 t
ORIGIN      
        1 acgcagacag caagctaata aggaaatgat tatgcgctac cgttcactat ctctacttat
       61 tccatgtgca ctgctgctga gtgcctgtac caccgtcacg ccagcttata aagataacgg
      121 tccccgcacc ggttcctgtg tccagggggg gcctgacagc gtggctcaac aattctatga
      181 ctaccgcatt caacatcgca gcaatgacat taccgccctg cgcccctatt tgagtgacaa
      241 actggcaacc cttcttagcg atgccagccg cgataatagt catcgtgaat tgttaagcag
      301 cgatccgttc tccagtcgaa ccaccttgcc agatagcgcc cacgttgcca gtgcatcgac
      361 tatccctaat cgtgatgccc gtaacattcc attgcgcgta gatctcaaac agggtgacca
      421 gggttggcag gatgaagtgt taatgattca ggaaggccag tgttgggtca tcgacgatgt
      481 gcgctactta ggcggcagcg ttcatgccac ggcgggtacg ctccgtcagt ccatcgagaa
      541 tcgctaacgc cttgatcaac caataaaccc cgtaaattgt ccggcatttc gtgaggaatg
      601 ccgacattta tgctcgccga ccaccgcccc cgttattttg tgctatgttt attgaataat
      661 gcgctttgct tttaactttt aaagcagaaa tattgcataa ttattctgtc aaaggtacta
      721 tcttcgacct caatagctat cagactgcca gtatacgagt gtcaatgagt attcaattaa
      781 acggcattaa ttgcttctac ggcgcgcatc aggcgctgtt cgatatcacg ctggattgcc
      841 cacagggcga aacgctggtg ttacttggcc ccagcggcgc gggtaaaagc tcgctgctgc
      901 gtgtactcaa tctgcttgag atgccacgct ccggtacgct caacattgca ggcaaccatt
      961 tcgatttcac caaaacaccc tctgacaaag cgattcgcga tttgcgtcgt aacgttggca
     1021 tggtgtttca gcaatacaac ctgtggccgc atctgaccgt gcagcaaaac ctgattgaag
     1081 cgccctgccg tgtactgggg ttgagtaaag atcaggcgct ggcccgtgca gaaaaactgc
     1141 tggaacgtct gcgtctcaaa ccttatagcg atcgttaccc gctgcatctt tctggtggtc
     1201 agcagcagcg tgttgctatt gcccgtgcgt tgatgatgga accgcaggta ctgctgttcg
     1261 atgaaccgac cgccgcactg gacccggaaa ttacggcaca aatcgtcagc atcattcgtg
     1321 agctggcaga aacgaatatt acccaggtga tcgtcaccca cgaagttgaa gtggcgcgta
     1381 aaaccgccag ccgagtggtg tatatggaaa atggtcatat cgtagaacaa ggcgacgcga
     1441 gctgctttac cgagccgcaa accgaagcat ttaaaaacta tctctctcac taagattcgg
     1501 gataacaaca atgaaaaaag ttctgattgc cgcgttaatt gcaggtttta gtctttccgc
     1561 cacagctgcc gaaaccattc gttttgctac cgaagcatcc tatcctccgt ttgaatcgat
     1621 tgatgcaaac aaccagatcg ttggttttga cgtcgacctg gcacaagcgc tgtgtaaaga
     1681 gattgatgca acctgcactt tctctaacca ggcgtttgac agcctgatcc caagcctgaa
     1741 attccgtcgc gtagaagccg tgatggcggg catggatatc actccggagc gtgaaaagca
     1801 ggtgctgttt accaccccgt actatgacaa ctctgccctg tttgtgggtc agcaaggcaa
     1861 atacaccagt gttgatcagc tgaaaggcaa aaaagtcggc gtacagaacg ggacgacaca
     1921 ccagaaattc attatggata agcacccgga aatcactacc gttccgtatg acagctacca
     1981 gaacgcaaaa ctggatctgc aaaacgggcg tatcgacggc gtcttcggtg acaccgcagt
     2041 ggtcactgag tggctgaaag ataacccgaa actggcggcg gtgggcgaca aagtgaccga
     2101 taaagattac ttcggcactg gcctcggcat cgcggtacgt cagggcaaca ctgagctgca
     2161 gcagaaactc aacactgcgc tggaaaaagt gaagaaagat ggcacttacg aaaccatcta
     2221 caacaaatgg ttccagaagt aattcctgat gaatgaattt tttcctttag caagcgccgc
     2281 cgggatgacc gtcggccttg ccgtttgtgc attgattgtc gggctggcgc tggcgatgtt
     2341 ctttgcggta tgggagtcgg caaaatggcg tcctgtcgcg tgggcaggtt cagcgctggt
     2401 aaccattctg cgtggcctgc cagaaattct ggtggtgctg tttatctatt ttggctcctc
     2461 gcagctgctg ctgacgcttt cggatggctt cactatcaat cttgggttcg tgcagatccc
     2521 agtgcagatg gacattgaga acttcgacgt tagcccgttc ctttgtggtg tcatcgctct
     2581 gtcactgctg tatgccgcct atgcctcgca aacgcttcgg ggcgcgttga aagcggtgcc
     2641 ggtgggtcag tgggagtctg gtcaggcgct ggggctgtcg aaatcggcta tctttttccg
     2701 tctggtgatg ccgcagatgt ggcgtcatgc gctgcctggc ctcggtaacc agtggctggt
     2761 gctgctgaaa gataccgcgc tggtcagttt gattagtgtg aatgatttaa tgctgcaaac
     2821 aaaaagcatc gctactcgta cccaggaacc atttacctgg tacattgtgg cggcggcgat
     2881 ttacctggtg atcaccctgc tcagtcagta cattctcaaa cgcattgacc tgcgcgcgac
     2941 acgttttgag cggaggccca gctaatgttt gagtatttac ccgaactgat gaaagggcta
     3001 cacaccagcc tgacgctaac cgttgcctcg ctgattgtgg cactgattct ggcattgatt
     3061 tttaccatca tcctgacgct gaaaacgccg gtgctggtgt ggctggtgcg gggttatatc
     3121 acgctgttta ccggtacgcc gctgctggtg cagatcttcc tgatttatta cgggccgggc
     3181 cagtttccga cgttgcagga gtatccggca ctgtggcatt tgttgtcaga accgtggtta
     3241 tgtgcgctga ttgcgttatc gctgaacagt gcggcgtata ccacgcagct gttttacggt
     3301 gcaattcgtg cgatcccaga aggtcagtgg cagtcctgta gcgccctggg aatgagcaaa
     3361 aaagatacgc tggcgctcct gctgccgtat gcctttaaac gctcgctctc ttcttattcc
     3421 aacgaagtgg tgctggtgtt caaaagtacc tctctggctt acaccattac gctgatggaa
     3481 gtgatgggat acagccagtt gttgtacgga cgcacctacg atgtaatggt gttcggtgcg
     3541 gcagggatta tttacctggt cgtcaacggc ctgctgacgc tgatgatgcg tctgatcgag
     3601 cgcaaagcac tggcattcga acggcggaat taatgtagct gcgtacattc gccggggggg
     3661 tttgtaggtc tgataagacg cgccagcgtc gcatcagaca tcagcacggt gcctgtgccg
     3721 gatgcggcgt ggacgcctta tccggcctac atagtgcata aattcttata atgaagacgg
     3781 acaactcact aagttatccg tcttttttta tttcatttaa attatttaat catatttatt
     3841 gcatataaat tcacttgatg gcattgttat cccatgccgc agacacggcc aaaaatcata
     3901 agattgacag acgggagttc catcatgaaa aagttagttc ttgccgcttt acttgcttcc
     3961 tttactttcg gtgcttctgc cgcagagaaa atcaattttg gcgtttcagc cacctatcca
     4021 ccctttgaat ctataggtgc taataatgag attgtcggct ttgatatcga tctggcaaaa
     4081 gccttgtgca aacaaatgca ggcagaatgt acttttacta atcacgcgtt cgacagcctg
     4141 atcccgtcac tgaaattcag aaaatatgac tccgtaatct ccggtatgga tatcacgccc
     4201 gagcgtagca agcaggtatc gtttaccacg ccctactatg aaaactcagc cgtcgtgatt
     4261 gccaaaaaag atacctacaa aacgtttgcc gatctgaaag gcaaacgtat tgggatggaa
     4321 aacggcacca cgcaccagaa atatattcag gatcagcacc ccgaagtgaa aactgtctcc
     4381 tatgacagtt atcagaatgc ctttatcgat ctgaaaaatg gtcgtattga tggggtattt
     4441 ggtgacacag cggtggtaaa cgaatggctg aaaaccaatc cgcaactggg cgttgctact
     4501 gaaaaagtga ccgatccgca atacttcggc accggtctgg gcatcgctgt acgtccggat
     4561 aacaaagctc tgctggaaaa actgaataac gcgctggcag caattaaagc tgacggtaca
     4621 tatcagaaaa tcaatgacca gtggttcccg cagtaattga gactgatgac aaaagtaaga
     4681 ttgcctgatg cgctgcgctt atctggccca catcaactct gcaatgtatt gatttagcat
     4741 gattttgtag gccggataag gcgtacacgc cgcatccggc atgaacaaag cgcacctgat
     4801 cagcaatctg aagccgcacc tgcggctttt ttattgcttc accagcagcg tcatcacttc
     4861 atagtgcgcg gtgtgcggga acatatcaaa aagctgtacc cgttcgatgc ggtaacctgg
     4921 cagttcgcgg atatctttcg ccatggtttg ggcgttacag ctggagtaga taataaaacg
     4981 cggtgccatc gttgagagat aatcacacag cggtttacca atgccgcggc gcggcgggtt
     5041 aaccagcacc agctccggca catccccctg agcggtggca aactgagtgg agtccagcgc
     5101 ctgaaattgc aaacgcgtta agcccagttc agcggctgac tgctttgcac aggcaatggc
     5161 ctctgatgca atttcgatcc cggttaactg catgtcaggc gtcgcgcagt gtaaaccaaa
     5221 gccacccaca ccgcagaaca gatcccacat atgtttaact ggcagctgcc gtacccagtc
     5281 gcgcgcggta gcgtacagct ggctggcgac cgccggatta gtctggaaga aactttgcgg
     5341 acggatccac agcggtacgt cattaaaacg ctctgccagc gcctgttgtt cagtcaggta
     5401 gatctccgtc tccccttcca taatcgccat atgtaccggc tgaatattga cggtaataac
     5461 tttcagttgc ggtagttgtt cctgtaacca cggcagcgcc ttacgcagtt gtgccagttt
     5521 ggtttcagaa cgcagtacaa agcgcagcat catgccgcca tcgctctggc tttcagtcag
     5581 cagaatgtat ttcagttcgc cacgcttacg cgccacgttg tagggcgtta accccgcacg
     5641 ggcgataaac ggttttagcg ccgcaaaaac gggcgcaaaa gaggcaggat aaagcgggca
     5701 gtcacaaagg tcttccggcg tgccatcgcg atgcagcata ccgagcagtg gtttttcaac
     5761 gctaccactc accaccattt tggctttatt acgaaaccct tgttccgggc ctgacaccgg
     5821 cgcgcaccat tcctcaaccg gaaaatcggc gagcagattt ttaagatcgg cggttttagc
     5881 ggagagttgc tctggaatcg gctgcgttat ccactgacag gaacgacagc gacccgcgtc
     5941 gtaaagtgcg cactgcatac attgaccttc acatcatcag gggcgacgat tatacacatt
     6001 attgcaactg gaagaaccgc cgactggtcg agggaacgaa cagcagcatc aggatgagca
     6061 tatccggcag cttctgcagc atcaggctat ggaagatttc acgttttgat tcaccgggaa
     6121 tgctgaacag ctccggataa ccgtacccca gcgaagccgc ccacaagtaa cttgcggcgg
     6181 tgatttgtgt cagcagatat agccagcgcg cccagcgacg ccctttcacc agtgaaaacg
     6241 cacaccagat ctcaatgaaa accagcacca gactgcttaa aaagaccagc gttaaactcc
     6301 aggtttgtac gctgcgatga atgaactcgc caataccgcg cacgcccagc gtattgaaaa
     6361 tcatcagcac gtcgaggcca cggatcataa taatggcgag cgccgccacc tgcaccagcg
     6421 caggcacatt caggcgagca tgagatgaag atgttttctt aaaaaatccc aacgtttcgt
     6481 cttccatgaa aacaatgccg cggcatgcgc ggcattatgt agccaggttg gcaaatttta
     6541 gtgtcttcag ccatgtcttg cacgctggat atcgcgtatc cgctgttttt ctgcgcgagc
     6601 catcagatac caggcgataa atccgacaat tccgaccgca ccgagaatta acgttgcaag
     6661 ggcgttgatt tccggattca ctcccatccg cacgctggaa aagaccagca tcggtaacgt
     6721 ggtggctccc ggcccggaaa caaagctggc gatcaccaga tcatcaagcg acaaagtaaa
     6781 agccagtaac cagccagaaa tgatcgcggg catgatcatc ggtagcgtaa tgacgaaaaa
     6841 cactttcagc ggcgtcgcac cgagatccat cgctgcttct tctatcgagc gatccagttc
     6901 ccgcaatctt gaagaaataa cgaccgccac ataagccgta caaaaagtga catgcgccag
     6961 ccagatggtg agcataccgc ggtccgcagg ccagccaatg gcatgagcaa gcgcgacgaa
     7021 taataacaac agcgacaagc ccgtgatgcc atctggcatc accagcggcg cggtgatcat
     7081 aaaggcaaaa ccatttgatc cgcgaaacct gccaaaacgc accagcacca ccgccgcaat
     7141 agtcccgagg atcgccgccg ccgttgccgc acaggcagca attgtcaggc ttaaaccaac
     7201 cgcactcatc atcgcgtcat cgcgcaataa ctcaccatac cagcgcgttg accagccggc
     7261 ccacaccgtc accagcttcg agctgttaaa cgaatagata accagcatca gcattggcgc
     7321 gtagagaaag gtaaagccca gcagcaaaat cacaatccgc cagggcgaac gaactaccgg
     7381 taaattattc atccgtgttc tcccacgctt ttttgctggt gtttgtggaa ccacattatc
     7441 ggcacaatta gcagcagcaa catgatgatc gctaccgccg aggccaccgg ccagtcgcgg
     7501 ttattgaaaa actcttgcca taacacgcgc ccgatcatga tgctgtccgg gccaccgagc
     7561 agttccggga tcacaaactc gcccacagcc gggataaaca ccagcatcga tccggcaata
     7621 atcccacctt tagtcagcgg cacgatcaca gtaaagaacg ttttcagcgg tcgtgcaccg
     7681 agatccagcg ctgcttccac cagcgaataa tcaatacgaa tcaacgcggt ataaatcggc
     7741 agtaccataa acggcacgta agcgtaaaca atgccgatat aaacggccag attggtatgc
     7801 agaatggtca gcggttgatc gataaccccc agccacagca gaaaattatt cagcacaccg
     7861 ttgtttttta atattcccat ccaggcataa acgcggatca gaaacgaggt ccacgacggc
     7921 aggatcacca gtagtaataa aatattacgg gtcgaaggct tactgtgcgc caccgcccac
     7981 gccagcggat agccgatcag taaacagcaa aatgtcgaaa tcgccgccac ctgtaacgac
     8041 tggagataag catcaaaata gagcggatcg tcggtcagtt gcagaaaatt accgagatta
     8101 agagtgatgg aaagttgccc gtcagcccac tccatcagtt cggtataagg tggaatagcg
     8161 cgcgccatct ctcccaggct tattttaaag acaatcagaa atggcagcag aaacagcaag
     8221 atcaaccaga tatatagcaa cgcaatgacc agtttgcgcc catgcttcat ttgcagctgc
     8281 gacaaccaca gcttaaatcc gcccggcggt ttcgactggg cagcaggttc aagtgtactc
     8341 atctttgctc cttaaaccgt cagcaccaca cagctgtcca cttcccagca caaacgcact
     8401 tcgtcgcccc aggtcggtaa ccctttacga tgacgatggg cgttttgtag ctgggcgctg
     8461 atcatctgcc cacttttcag tcgaacgtga tacaccgaaa gatcgccgag ataggcaatg
     8521 tgtatcacct cccccaccgc gaagttacaa ccattggcgg gcggctcttc gcaaagcatg
     8581 attttttccg ggcgcagcgc tacgtgtacc ggcacgttat cgaccactga agcatccgca
     8641 tcgactttca gtggatgcac cagccccggc gaatcaagca ccaggccatc ttcctgacgc
     8701 tctttgagta caccttcaaa gacattaacc gaaccgataa attcagcgct gtagcgggta
     8761 gttggatgct cgtagatctc ttccggctcg ccaatctgga caaatttccc gcgattcata
     8821 atggcgatac gccccgccat ggtcatcgcc tcttcctgat cgtgggtgac catcacacaa
     8881 gtcacaccga cgcgctccag aatatccacc acttcaagct gcatccggtc acgcagcttt
     8941 ttatccagcg cgcccatcgg ctcatcgagc agtaatagtt tcgggcgttt ggcaaggctt
     9001 cgggccaggg ccacacgctg tcgctgacca ccggaaagct gatgcggttt gcgtttggcg
     9061 aactcctgca tgtgcaccag gccgagcatc tcatggaccc gactggcaat ttccgctttc
     9121 ggtagtttgt cctgtttcag gccaaaagcg atgttctgtt ccacggtcat atgcggaaac
     9181 agcgcgtaag actgaaacat catattgatg gggcgcaggt aaggcggcac ctgtgacaaa
     9241 tcgacgccat cgagcattat ctgcccggca gaaggttgtt caaaacctgc cagcatacgc
     9301 agcagcgtgg acttgccaca gccggatgcg cccagcagcg cgaagatttc acctttgtag
     9361 atggtcaggc tgacatcatc gaccgcatgc tgaccatcgt aggatttggt gaggttgcgg
     9421 atttctaata gcggcgtcag cgccttacgg gttttcgcct gcgggcgagg gatagcgtca
     9481 ttcacggctg ctctccggca ttgaatgtgt gcaagcgcac catgatggtg cgaattgccg
     9541 gctggtgtgg tgcgcccgcc cggcatctaa ggctgcggat tattttccgc tcttcacttt
     9601 ggtccacgcg cgggtgcgca cacggtcgat tttcggatcc tgtactttca gagtgaatag
     9661 cttcgcacga acgtccgccg gaggataaat acccgggttc tcacggactt ccgcactcac
     9721 cagcggcgtg gctgctttat tggcgttggc atagaacaca tggtcggaaa tatgcgctac
     9781 tacatccggg cgcagcaggt aattcaggaa ctgataggct tcgtctttgt ttttggcatc
     9841 cgcaggcatg gcgaatacat caaagaacgc catcgcccct tcttttggaa tcgagaacga
     9901 gacattcact ccattcttcg cttccttcgc gcggtttgac gcctgccaga catcacctgc
     9961 ccagccgata gcgacgcaaa tatcgccgtt tgccaggtcg ttaatgtatt gagatgaatg
    10021 gaaataacga atgttcgggc gcagctttaa cagcagatcg gttgccggtc cggtgtaatc
    10081 atccgcttta gtgctgttgg gatctttgcc gagataattc aacacggtag caaaaacttc
    10141 ttctggcgca tccaggaaag agacaccgca gcttttcagt ttttccagat tttcaggttt
    10201 gaggatcaag tcccagctat cgacgggcgc gttttcgccc agcacagctt taactttatc
    10261 aacgttatag ccaatcccgg tcgtcgccca catatagggc atagcaaatt tattgtcggg
    10321 atcgtgtttg gcgaccagct tcagcagctc cgggtcgaga ttcttccact ccggcaattt
    10381 gcttttgtcc agcggctgga aaactcccgc agtcaactgg cgttcaagaa agctggcaga
    10441 tggaaccacc aagtcaaagc cggtgctacc cgccattaat ttgccttcca gtacttcgtt
    10501 agagtcgaaa acatcgtaga cgactttaat accggtttct ttttcaaaat tggccaccgt
    10561 gtccggggcg atataatcag accagttata aatgtggagt gttttttgtt cagccgcgag
    10621 cgtgccgaca gagacggcca tcagagcacc cgcagccaga cccgaaagcc attttttatt
    10681 taaggcggtc atatctcttt ccttctgaaa gttcgttaac aaacgaacaa aagttgtcac
    10741 actaaaagat atgcaataaa cgtgcatatt ttttatcggc ggggtgtaag aaaagagaac
    10801 tctctcccgg ccagcactgc tcgctatata aaagcatcgc aagaataact ttagccaggg
    10861 tttgagacta tagctcggat taaaaaacgg tatttaacaa atttttatgc gtatttgctc
    10921 tatgtgataa agccaaatcg acacaagaga agtgaaataa tctttaagaa aaggttaaaa
    10981 acagcagata attttgcagc gcgcagccac tgcaccagcg actgcgtgct cgaaaggagt
    11041 tatcagtgga ggaaatagtt ttcggtaacg ttgttttgcc cttcatcgtc agtcggtaac
    11101 agcagttgat gggcattagc ttcaagaatg accatcgaaa tctgctcttc gctctgacgc
    11161 acgaaccatg caaactgctc ataggttacg ccctgcataa cggataaaga ctggcaaacc
    11221 accagctttg gcagattatc atcctgcata tcaagaaatg ctttcacggt cagcgaactg
    11281 gcattgatgg cagataaatc cgccgccagc ggcaatacgg ctgaaggtcg gacttccgcc
    11341 atggcagaaa acaggatcgt gttatcgatc agatcgattt tggcatcaaa gacaccgtcg
    11401 aaattctgca tatggggcag atgcagagcc tgacagttat cacattcaaa aaaactcatc
    11461 cccaggtcat cgagccattg acgcagcgta tccagaccag gaacgaccag cgatgtcata
    11521 attttgtaca acctctttcg ataaaaagac cggcacagct tacgcaaaaa gcgcaggcaa
    11581 aaccatgatc agtaatgtga ttgcgactaa ccacccgttt tcaggcaata ttctgtcgta
    11641 gcgtggcgtt cgatccagcg gatcatttta cccgcgatgt cgataccagt ggttttttct
    11701 attccttcca gccccggcga cgcattcact tccatcacca acggcccgcg attagcacgc
    11761 agaatatcca caccagcaac gtccagcgcc atcgttcgcg cggcttttat cgcgatttca
    11821 cgctcctgtg gtgtgatact ggcgacgctt gccgcgccgc cacgatgcaa attggaacga
    11881 aaatcgccct ctttcgcccg ccgttcaatc gcagcgacca cttcatcgcc aacaaccaga
    11941 cagcggatat cgcacccttg cgcctctttg atatattcct gcaccagaat atgcgcgttc
    12001 agaccgcgga aagcgtcaat cacgctttcc gccgcctgac gcgtctccgc cagcacgacg
    12061 ccaattccct gcgtgccttc aaccaacttg accaccagcg gcgcaccacc gaccatgtcg
    12121 attaaatcgc tggtatcatc cggcgaatgc gcaatgcccg tgacaggcag gtcgatgccc
    12181 tgacgcgcca gcagttgcat ggaacgcaat ttgtcacgcg cccgggcaat ggcgaccgac
    12241 tcattgagcg gatagctccc cagcatctcg aactggcgca gtgccgccgt cccataaaag
    12301 gtaatggcgg tgccaatacg cgggatcact gcgtcaaaat ggggtaactt gcggcctttg
    12361 tagtgaatag aagacgccgc aggatttatg ttcatgtagc aagaaagcgg atcaagaatt
    12421 tcaaccaggt gaccgcgctg tatagcggct tcacgcagcc gcttacacga atagagcgtt
    12481 ccatcccggg acaatatggc aattttcacc ctgcacctct ctgtcagacc tggtcaaaag
    12541 cccgcgtatc atacatcgac gtggcggttt tagcgcgtcg cccaaccctg tttgtgcaaa
    12601 taatccagaa taaatgggcg gctttcttta atgattgttc ggcggatatg atcgctccag
    12661 gtatcccggc gattattgct gccacgggtg aggtaatatt ccgccagttg ctcgtcatac
    12721 tgcgccagtg cgtctttatc cagcggttga tagctgtttt catgcaccaa aatggaggcc
    12781 ggtaaacgcg gcttgagatc cggattatcc gcaggccagc caaggcacag cccaaacagc
    12841 ggcagaacat gctgcggtaa tttcagcagt ttcgtcaccg cttcaatatt attgcgcagg
    12901 ccgccgatat ataccccg
//
LOCUS       DAZSBF010000111        12871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZSBF010000111 DAZSBF010000000
VERSION     DAZSBF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.112"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..695)
                     /locus_tag="ACHXWD_002508"
     CDS             complement(<1..695)
                     /locus_tag="ACHXWD_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7621400.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            951..1106
                     /gene="rseD"
                     /locus_tag="ACHXWD_002509"
     CDS             951..1106
                     /gene="rseD"
                     /locus_tag="ACHXWD_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR7621401.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1103..1678
                     /gene="rpoE"
                     /locus_tag="ACHXWD_002510"
     CDS             1103..1678
                     /gene="rpoE"
                     /locus_tag="ACHXWD_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR7621402.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1711..2361
                     /gene="rseA"
                     /locus_tag="ACHXWD_002511"
     CDS             1711..2361
                     /gene="rseA"
                     /locus_tag="ACHXWD_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR7621403.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2361..3317
                     /gene="rseB"
                     /locus_tag="ACHXWD_002512"
     CDS             2361..3317
                     /gene="rseB"
                     /locus_tag="ACHXWD_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR7621404.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3314..3793
                     /gene="rseC"
                     /locus_tag="ACHXWD_002513"
     CDS             3314..3793
                     /gene="rseC"
                     /locus_tag="ACHXWD_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR7621405.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3991..5790
                     /gene="lepA"
                     /locus_tag="ACHXWD_002514"
     CDS             3991..5790
                     /gene="lepA"
                     /locus_tag="ACHXWD_002514"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR7621406.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5806..6780
                     /gene="lepB"
                     /locus_tag="ACHXWD_002515"
     CDS             5806..6780
                     /gene="lepB"
                     /locus_tag="ACHXWD_002515"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR7621407.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7053..7733
                     /gene="rnc"
                     /locus_tag="ACHXWD_002516"
     CDS             7053..7733
                     /gene="rnc"
                     /locus_tag="ACHXWD_002516"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR7621408.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7730..8635
                     /gene="era"
                     /locus_tag="ACHXWD_002517"
     CDS             7730..8635
                     /gene="era"
                     /locus_tag="ACHXWD_002517"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR7621409.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8647..9375
                     /gene="recO"
                     /locus_tag="ACHXWD_002518"
     CDS             8647..9375
                     /gene="recO"
                     /locus_tag="ACHXWD_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR7621410.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9387..10118
                     /gene="pdxJ"
                     /locus_tag="ACHXWD_002519"
     CDS             9387..10118
                     /gene="pdxJ"
                     /locus_tag="ACHXWD_002519"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR7621411.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10118..10498
                     /gene="acpS"
                     /locus_tag="ACHXWD_002520"
     CDS             10118..10498
                     /gene="acpS"
                     /locus_tag="ACHXWD_002520"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR7621412.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10720..10800
                     /gene="shoB"
                     /locus_tag="ACHXWD_002521"
     CDS             10720..10800
                     /gene="shoB"
                     /locus_tag="ACHXWD_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR7621413.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10994..11254)
                     /gene="yfhL"
                     /locus_tag="ACHXWD_002522"
     CDS             complement(10994..11254)
                     /gene="yfhL"
                     /locus_tag="ACHXWD_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR7621414.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11469..12659
                     /locus_tag="ACHXWD_002523"
                     /pseudo
     CDS             11469..12659
                     /locus_tag="ACHXWD_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         3278 a         3147 c         3353 g         3093 t
ORIGIN      
        1 cccgcgcgcc acgccatagc aatgccatcg ccagaagaaa tatccggatt ggtggtgtac
       61 tgatacacct tcgacgcacc gccggttgcc agtaccactg cttttgcgtg gcaggtttcc
      121 accgtttctt tatttcggtt ccatacccac gcgcctacaa cccgtcgcgt gcccggcagg
      181 ccaattttgt cagagacaat cagatcaact gcgttgctgc gctccagcac acgaatattc
      241 ggatggttct gcgccttgct caccagcgtg gattgtactt ctctaccggt ggcgtcggcg
      301 gcatgaagaa tacgacggtg actatgtcca ccttcacggg tcagatggta gctttcttcg
      361 ccattcggtt gaacgtgggt atcaaacaaa accccctggt caattaacca ttgcacacag
      421 gaacgagcat tgctggcgac aaattcaact gcatggcgat cgcaaatacc agccccggca
      481 atcaatgtgt cttccacatg cgagtcaatg ctgtcagttt catcaaacac ggcggcaata
      541 ccgccctggg cataaaatgt tgaaccttcc gttaccgggc ctttacttag aacgatgacc
      601 tgatgctggt cagccaggcg tagcgccagt gaaagtccgg ctgcgccgct accaataatc
      661 aacacgtcac atgaatgttc agggagagta ttcattttct ttgtttaatt tactaaacat
      721 tgtttggtca gcatagcatc atgttgtgcg aataaacacc tgctatttta atatttgtta
      781 cagtggctaa acacgttgac gcagggcggc gagaaaaaga gaagttactg gctggtggag
      841 gattaggtgg tgaaataaaa aggccgttgg gttactcttc aggcagttaa atgggcattt
      901 ctacacagat aatgcgatgt tcagattctg tagacttata atgatagata atgatccgtc
      961 tacagcatga caaacaaaaa cagatgcgtt acggaacttt acaaaaacga gacactctaa
     1021 ccctttgctt gctcaaattg cagctaatgg agtggcgttt cgatagcgcg tggaaatttg
     1081 gtttggggag actttacctc ggatgagcga gcagttaacg gaccaggtcc tggttgaacg
     1141 ggtccagaag ggagatcaga aagcctttaa cttactggta gtgcgctatc agcataaagt
     1201 ggcgagtctg gtttcccgct atgtgccgtc gggtgatgtt cccgatgtgg tacaagaagc
     1261 ttttattaaa gcctatcgtg cgctggattc gttccgggga gatagcgctt tttatacatg
     1321 gctgtatcgg attgctgtaa atacagcgaa aaattacctg gttgctcagg ggcgtcgtcc
     1381 accttccagt gatgtggatg ccattgaagc tgaaaacttc gaaagtggcg gcgcgttgaa
     1441 agaaatttcg aaccctgaga acttaatgtt gtcagaagaa ctgagacaga tagttttccg
     1501 aactattgag tccctcccgg aagatttgcg catggcaata accttgcggg agctggatgg
     1561 cctgagctat gaagagatag ccgctatcat ggattgtccg gtaggtacgg tgcgttcacg
     1621 tatcttccga gcgagggaag ctattgataa caaagttcaa ccgcttatca ggcgttgacg
     1681 atagcgggat actggataag ggtattaggc atgcagaaag aacaactttc cgctttaatg
     1741 gatggcgaaa cgctggatag tgagctgctt aacgaactgg ctcataaccc agaaatgcag
     1801 aaaacctggg aaagctatca cttaatccgt gattcaatgc ggggtgatac tcccgaggtg
     1861 ctccatttcg atatctcttc acgcgtgatg gccgccattg aagaagagcc agtacgtcaa
     1921 ccggcgacat tgatcccgga agcccagcct gcgccgcatc aatggcacaa aatgccattc
     1981 tggcagaaag tacgtccgtg ggcggcacag cttacccaaa tgggcgtagc agcatgcgta
     2041 tcgcttgcag ttatcgttgg cgtccagcac tataatggac aatctgaaac gtcccagcag
     2101 cccgaaacgc cggtatttaa tacactgccg atgatgggta aagccagccc ggtaagcctg
     2161 ggagtacctt ctgaagcgac cgcaaacaat ggtcaacagc agcaggtaca ggagcagcgt
     2221 cgtcgcatta atgcaatgtt gcaggattac gaactgcaac gccgactcca ctctgaacag
     2281 cttcagtttg agcaggcgca aacccagcaa gccgctgtac aggtgccagg acttcaaact
     2341 ttaggaacgc aatcgcagta atgaagcaac tttggtttgc catgtcatta gtgacaggta
     2401 gcctgttatt ctctgctaac gcctcggcca ctcccgcgtc cggggcgtta ttacagcaga
     2461 tgaacctggc cagtcagtca ctgaattacg agctgtcatt catcagcatc aataaacagg
     2521 gtgttgagtc tctgcgttat cgacatgcac gcctcgataa ccgtcctctt gcacaattgt
     2581 tgcaaatgga tggcccgcgc cgggaagtgg tacagcgcgg caatgaaatc agctattttg
     2641 aaccgggact tgaaccgttc acgcttaatg gcgattacat tgttgattct ctgccatcgc
     2701 ttatctatac cgatttcaaa cgcctttctc cttactacga ctttatctcc gtcgggcgca
     2761 cgcgtattgc tgatcgtctt tgcgaagtca ttcgcgtggt tgcccgagat ggtacacgct
     2821 acagctacat cgtgtggatg gacactgaat cgaaattacc gatgagggtt gatctccttg
     2881 atcgcgatgg tgaaacgctg gaacaatttc gcgtgatttc ttttaacgtc aatcaggata
     2941 tcagcagcag tatgcagacg ctggcgaagg caaatttgcc gccgttgctt tctgttcctg
     3001 taggtgaaaa agctaaattc agctggacgc caacctggtt gccacagggt tttagcgaag
     3061 tttccagtag tcgacgtccg ctaccgacga tggacaacat gcctatcgaa tcacgtctct
     3121 attccgacgg attattcagc ttctcggtaa acgttaaccg cgctacgcca tcgagcaccg
     3181 atcagatgtt gcgcaccgga cgcagaaccg tcagtacaag cgtacgtgat aacgccgaaa
     3241 tcaccattgt cggtgaactg ccgccgcaaa cggcgaaacg cattgccgag aatattaagt
     3301 tcggggcagc gcaatgatca aagagtgggc taccgtcgtc tcctggcaaa acgggcaggc
     3361 gctggtcagt tgtgatgtta aagcctcatg cagcagctgc gcttcacgcg ccggttgcgg
     3421 tagccgcgtg ttgaataaac ttggcccgca aaccacgcat accattgtcg taccctgtga
     3481 tgaaccgtta gtgccggggc aaaaagtgga attggggatc gccgaaggca gcctgcttag
     3541 ctccgcatta ctggtttata tgtcgccgct ggtgggatta ttccttatcg cttcgctatt
     3601 tcagctactc tttgcttctg acgttgcagc attatgtggt gcgatccttg gcggtattgg
     3661 cgggtttctg attgcgcgcg gttactcgcg taagtttgct gcccgggcag aatggcaacc
     3721 gatcatatta agcgtggcat tgccgcctga ccttgtgcga tttgaaacat cttcggaaga
     3781 cgcgagccag tgatttcact ggctttgttg cattttgcat aacaaatgcg aggacgtttc
     3841 cagataccac aatccatacg gtatgaacat ttcctcgcct caatgttgta gtgtagaatg
     3901 cggcgtttct attaatacag acgttaagct cagaacagcg acttctaaaa gcctggttaa
     3961 ccgggcatta aggcacaata atcatacttt atgaagaata tacgtaactt ttcgatcata
     4021 gctcacattg accacggtaa atcgacgctg tctgaccgta ttatccagat ctgcggtggc
     4081 ctgtctgacc gtgaaatgga ggcgcaggtt ctcgattcca tggatcttga gcgtgagcgt
     4141 ggcattacca tcaaagcgca aagcgtgacg ctggactaca aagcgtctga cggcgaaacc
     4201 tatcagctta actttatcga caccccgggc cacgtagact tctcctatga agtttcccgt
     4261 tcgctggctg cctgtgaagg tgcattgctg gtggtcgacg ccgggcaggg cgtggaagcg
     4321 caaaccctgg caaactgcta caccgccatg gaaatggatc tcgaagtggt gccagtactg
     4381 aacaagattg acctgccggc agccgatcct gaacgcgtgg cggaagaaat tgaagatatc
     4441 gtcggcatcg acgccactga cgcggtgcgc tgttcagcga aaaccggcgt tggcgtgcag
     4501 gacgttctcg aacgtctggt gcgcgacatt ccgccgccgg aaggcgatcc ggaaggctcg
     4561 ttgcaggcac taattatcga ctcatggttc gacaactacc tgggcgttgt ttcacttatc
     4621 cgtattaaaa acggcaccct gcgtaagggc gacaaagtga aagtcatgag taccgggcag
     4681 acctataacg ccgaccgtct gggcatcttc acgccgaaac aggttgaccg cactgaactg
     4741 aaatgtggcg aagtaggctg gctggtatgt gcgattaaag atatccatgg cgctccagtc
     4801 ggcgatacct taacgctggc gcgtaatccg gcagaaaagg cgctgcctgg ctttaagaaa
     4861 gtcaaaccgc aggtatacgc cggtctgttc ccggtaagtt ccgacgacta tgaagccttc
     4921 cgtgacgcgc tgggtaaact cagcctgaat gatgcctcac tgttctatga gccggaaagc
     4981 tccagcgcgc tgggctttgg tttccgctgc ggcttccttg gcctgctgca catggagatc
     5041 atccaggaac gtctggaacg tgaatacgat ctggatctga tcaccactgc gccgaccgta
     5101 gtgtatgaag ttgaaaccac gtcaagggaa gttatctacg tcgacagccc atccaagctg
     5161 cctgcggtaa ataacattta cgaactgcgc gagccgattg cagagtgtca catgctgctg
     5221 ccgcaggcat atctcggcaa cgttattacg ctgtgcgtag aaaaacgcgg cgtgcagacc
     5281 aatatggttt accacggtaa tcaggtggcg ctgacgtacg agatcccgat ggcggaagtg
     5341 gtgctcgact tcttcgatcg cctgaaatct acctcgcgtg gttatgcgtc tctggattac
     5401 aacttcaaac gcttccaggc gtccgacatg gtacgtgtag acgtattaat caacggtgaa
     5461 cgtgttgatg cgttggcgct gatcacccac cgtgataatt cgcaaaaccg cggtcgcgag
     5521 ttggtggaga agatgaaaga tctgatccca cgccagcagt ttgatatcgc cattcaggca
     5581 gcgattggta cgcacattat tgcgcgatcc accgtgaaac agctgcgtaa aaacgtactg
     5641 gctaaatgtt atggcggcga tatcagccgt aagaaaaagc tgctgcagaa gcagaaagaa
     5701 ggtaagaaac gcatgaagca gatcggtaac gtcgagctgc cgcaggaagc gttcctcgcc
     5761 attctgcacg tcggcaaaga caacaaataa cccttaggag ttggcatggc gaatatgttt
     5821 gccctgattc tggtgattgc cacactggtg acgggcattt tatggtgcgt ggataaattc
     5881 tttttcgcac ctaaacggcg ggaacgtcag gcagcggcgc aggcggctgc cggtgactca
     5941 ctggataaag caacgttgaa aaaggttgca ccgaagcttg gctggctgga aaccggagct
     6001 tctgtttttc cggtgctggc tatcgtattg attgtacgtt cgtttattta tgaaccgttc
     6061 cagatcccgt caggttcgat gatgccgact ctgttaatcg gtgattttat tctggtagag
     6121 aagtttgctt atggcattaa agatcctatc taccagaaaa cgctgatcga aaccggtcat
     6181 ccgaaacgcg gcgatatcgt ggtctttaaa tatccggaag atccaaagct tgattacatc
     6241 aagcgcgcgg tgggtttacc gggcgataaa gtcacttacg atccggtctc aaaagagctg
     6301 acgattcaac cgggatgcag ttccggccag gcgtgtgaaa acgcgctgcc ggtcacctac
     6361 tcaaacgtgg aaccgagcga tttcgttcag accttctcac gccgtaatgg tggggaagcg
     6421 accagcggat tctttgaagt gccgaaaaac gaaaccaaag aaaatggaat tcgtctttcc
     6481 gagcgtaaag agacactggg tgatgtgacg caccgaattc tgacagtgcc gattgcgcag
     6541 gaccaggtgg ggatgtatta ccagcagcca gggcaacaac tggcaacctg gattgttccg
     6601 ccgggacaat acttcatgat gggcgacaac cgcgacaaca gcgcggacag ccgttactgg
     6661 ggctttgtgc ctgaagcgaa tctggtcggt cgggcaacgg ctatctggat gagtttcgat
     6721 aagcaagaag gcgaatggcc gacaggtgtg cgcttaagtc gcattggcgg catccattaa
     6781 tagcccatct tcgttcacgt tgtcgccgtt atggcgacaa cgtgaattat ttatgagata
     6841 aatctcccgt ggctaacgac atcccccgtc gttgtgtata gaatattccc ccgaagttta
     6901 aggttggcac ctccaggttg ccacggcaca cgaaacagcg ttggtcccca tataccggta
     6961 aactgaaact gcagcgaagc agttagcaga gccatgtata tcaggtctgt ttcgtgtgct
     7021 gaattgttga cgcatttatt tattggtatc gcatgaaccc catcgtaatt aatcggcttc
     7081 aacggaagct gggctacact tttaatcatc aggaactgtt gcagcaggca ttaactcatc
     7141 gtagtgccag cagcaaacat aacgagcgtt tagaattttt aggcgactct attctgagct
     7201 acgttatcgc caatgcgctt tatcaccgtt tccctcgtgt ggatgaaggc gatatgagcc
     7261 ggatgcgcgc cacgctggtc cgtggcaata cgctggcgga actggcgcgc gaatttgagt
     7321 taggcgagtg cttacgttta gggccaggtg aacttaaaag cggtggattt cgtcgtgagt
     7381 caattctcgc cgataccgtc gaagcattaa ttggtggcgt attcctcgac agtgatattc
     7441 aaaccgtcga gaaattaatc ctcaactggt atcaaactcg tttggacgaa attagcccag
     7501 gcgataaaca aaaagatccg aaaacgcgct tgcaagaata tttgcagggt cgccatctgc
     7561 cgctgccgac ttatctggta gtccaggtac gtggcgaagc gcacgatcag gaatttacta
     7621 tccactgcca ggtcagcggc ctgagtgaac cggtggttgg cacaggttca agccgtcgta
     7681 aggctgagca ggctgccgcc gaacaggcgt tgaaaaaact ggagctggaa tgagcatcga
     7741 taaaagttac tgcggattta ttgccatcgt cggacgtccg aacgttggca aatccacatt
     7801 gttgaacaaa ctgctggggc agaagatctc catcacctcc cgcaaagcgc aaacaactcg
     7861 tcaccgcatt gtggggatcc atactgaagg cgcgtatcag gcgatctacg ttgatacccc
     7921 tggcctgcat atggaagaaa aacgcgctat taaccgcctg atgaacaaag cggcgagcag
     7981 ctctattggc gacgttgagc tggtgatttt tgtcgttgaa ggcacccgct ggacgccgga
     8041 cgacgaaatg gtgctcaaca aactgcgcga tggcaaagcg ccggtgatcc tcgcggtgaa
     8101 caaagtggat aacgtgcagg aaaaagccga tctgctgccg cacttacagt tcctggcaag
     8161 tcagatgaac ttcctcgata tcgtgccgat ctctgctgaa accgggctga atgtcgacac
     8221 tatcgcagca atcgtgcgta agcatctgcc tgaagcgacc catcacttcc cggaagatta
     8281 catcaccgat cgctcacagc gttttatggc gtctgaaatc atccgcgaaa aactgatgcg
     8341 tttcctcggc gctgaactgc cgtactccgt gaccgtggag atcgaacgtt tcgtctctaa
     8401 cgaacgcggt ggttatgaca tcaatggttt gattctcgtt gagcgtgaag ggcagaagaa
     8461 gatggttatt ggcaacaaag gggccaagat caaaaccatc gggattgaag cgcgtaaaga
     8521 catgcaggaa atgttcgaag cgcctgtcca ccttgaactg tgggtaaaag tgaaatccgg
     8581 ttgggccgac gacgaacgcg cactgcgcag tctcggttac gttgacgatc tttaagagtc
     8641 actccgatgg aaggctggca gcgcgcattt gtcctgcata gtcgcccgtg gagcgaaacc
     8701 agcctgatgc tggacgtctt cacggaggaa tcgggtcgcg tgcgtctggt tgccaaaggc
     8761 gcacgctcta aacgctctac tctgaaaggt gcattacagc ctttcacccc tctcttgcta
     8821 cgtttcggcg ggcgtggcga agtcaaaacg ctgcgcagtg ctgaagctgt ctcgctggcg
     8881 ctgccattaa gcggtatcac gctttacagc ggtctgtaca tcaacgaact tctctcccgc
     8941 gtactggaat acgagacgcg cttctctgaa ctctttttcg attacttgca ctgcattcag
     9001 tctcttgcag gtgccactgg tacgccagaa cccgcgctgc gccgctttga actggcactg
     9061 ctcgggcatc tgggctatgg cgtcaatttt acccattgcg cgggtagcgg cgagccggta
     9121 gatgacacca tgacgtatcg ttatcgcgaa gaaaaagggt ttatcgcaag cgtcgttatc
     9181 gacaataaaa cgttcaccgg aaggcagtta aaagcgttaa acgcgcggga atttcctgac
     9241 gcagacacac tacgcgccgc gaaacgcttt acccgcatgg cgcttaagcc gtatcttggc
     9301 ggtaaaccat taaagagcag ggaactgttc cggcagttta tgcctaagcg aacggtgaaa
     9361 acacattatg aatgatgagg attgtcatgg ctgaattact gttaggcgtc aacattgacc
     9421 atattgcaac gctgcgtaac gcacgcggta ctgcttaccc ggatccggtg caggcggcgt
     9481 ttattgccga gcaggcggga gcggacggca ttaccgtgca tttacgtgaa gatcgccgcc
     9541 acattactga ccgcgatgtg cgcatcctgc ggcagacgct ggatacccga atgaatctgg
     9601 agatggcagt gaccgaagag atgctggcga tcgccgttga gacgaagcca catttttgct
     9661 gcctggtacc ggaaaagcgt caggaagtaa caaccgaagg cggcctggac gttgcagggc
     9721 agcgtgaaaa aatgcgcgat gcctgcaaac gtctgacaga tgccgggatt cagatttctc
     9781 tctttatcga cgccgatgaa gagcagatca aagctgcggc agaggttggc gcaccgttta
     9841 tcgagatcca caccggttgc tatgctgatg ccaaaactga cgccgaacag gcgcaagagc
     9901 tggtgcgtat cgccaaagcc gcgacctttg ccgctagtct cggtctgaaa gttaacgccg
     9961 gacacggtct gacctatcac aacgtgaaag ccattgccgc catccctgag atgcatgaac
    10021 tgaatatcgg tcatgccatt attggccgtg cagtgatgac cggactgaaa gatgcggtgg
    10081 cagaaatgaa gcgtctgatg ctggaagcgc gtggctaatg gcaatattag gtttaggcac
    10141 ggatatcgta gagatcgctc gcatagaagc agtgatcgcc cgatccggtg agcgcctggc
    10201 acgccgcgta ttaagcgata acgaatgggc aatctggaaa acgcaccacc agccggtgcg
    10261 ttttctggcg aagcgttttg ctgtgaaaga agccgcagca aaagcgtttg gcaccgggat
    10321 ccgcaatggt ctggcgttta atcaatttga agtattcaat gatgagctcg gcaaaccacg
    10381 gctacggcta tggggtgagg cattaaaact ggcggaaaag ctcggcgttg taaatatgca
    10441 tgtaacgctg gcagatgaac gccactatgc ttgtgccacg gtaattattg aaagttaaaa
    10501 taataccgtt ctctttaaaa agtaaaaccc ccgaactggt aaggaacggg ggttttactt
    10561 tttaacttta attttgtgca gcatgcaccc tcaacaacag cgattatgga tcagtgtact
    10621 tatcttttac aagcaacttg caatctttag cataaaaact cgagccttta cgaagaaagc
    10681 aatactgaac ggctgcattc cttgtttaag gaaaaacgaa tgactgattg ccgatacctg
    10741 attaaacggg tcatcaaaat catcattgct gttttacagc tgatccttct gttcttataa
    10801 cacaaggaaa cgtacttaag gtgcgtccgg tgaaccaatc ggacgcgcct ttaataacta
    10861 taaataagtg tcttggcaga tactatataa attaacttag tgaatgatta tgctaatgtc
    10921 atcaattaaa taaatataat ggcgttaagg cttcccagta atataattaa tactctactt
    10981 ccagagtaga atattaaatt ttatccgcgt ggtgcatcag cacaaattta tcccacaact
    11041 gttcttctgt ctcgacatgc gccggatctt tcacaatagt attggggatc gggcacacct
    11101 tctggcaggt tggtgtctcg tagtgcccta cgcattcggt acacttatcg ctgttaatct
    11161 cgtagatatg atctcccatt gaaatcgcct cattcgggca ttcgggttca cacatatcac
    11221 aattaataca gcgtttagta attagtaaag acatttcaat ggattaccgt taaatcattt
    11281 taaaatcagt aagttgtatc gagtttgtat gctttactgt cattaactta ctgtatgttg
    11341 atccagtgta tttaaccttg ataaactcag tccagcaaca caaaaccgca acacattgca
    11401 ttttgtcccg tagaaaagac ttgtatgtgt gagcttgttt tctgcgccta cgcagataag
    11461 gattgagaat gccgcgcact gtaacacata atccggatag ccccaataat gacgatgttt
    11521 tagccgcatc tgaaaaatgg gacgcctgta aaccccccta taccagcgca cacatgaaaa
    11581 tctgtgttgc tgccgccaaa atcatcctcg ctgcttccgg cgtggctcgc cgttccaaat
    11641 acgaaaaaga gaactatctc cgtatcgatt tcagcaaagc cggtaaggtt acattttacg
    11701 ccgagtttcc aaaaaagatg ggcctcaaag gtaaaaagct cggcgagtgg ccggagctcg
    11761 ctatccagct ggcgcgcgaa aaagcactag gtatggctga cggtggactg cgggcagagt
    11821 ccgtacatgc agcgctggaa atgtaccggg atgacctcaa agccaaagta gcccggcaga
    11881 agctgagccc ggacagtttc acaacctacg gggtgcgtat cgaccggatt aaagcaacgt
    11941 tcggcgagcg tgaggtgttc agcgacgtaa catacaatcg gctggtggaa gtgctggacg
    12001 agtggatcgc cactcgctcg aacaataacg ccctggagtt gtttgccgag ctccgtcggt
    12061 tctggaagtt ctgcgcacct actctttgca acggccgcaa tgttgccgcc agtctgccag
    12121 atgattatgt ttcctcccgc gtacagaaac ctacccccac acggcttttt accgatattg
    12181 aatcaatcgc ccgactctgg ctcaatgttg ctgcctgcac ctctgtacac cagaagaatg
    12241 ctgttcgctt catgatcatc actggtgttc gtccgattaa tgtccataac ctgcgctggg
    12301 actacgttta cgaggaggct ggtgaaattg tttatccgga aggggttatc ggcatgcgag
    12361 gggctatgaa aacacaaaag gctttccgcc tgccgataac gcctgagatc cggcggatta
    12421 tcgacgagca gaaagcctgg cgtgattcag ttcctgagtg caacagggat tatgtatttt
    12481 tgcagccacg tgatccaatg cagccatttt caaaacgatc actggataag ctggtgaaaa
    12541 catacagccc ggacggggct gtaaaaggaa taaaacatga tgggactgtt aaagggaaag
    12601 acggtgcatt taatacgatg tgccgtaaat tccttaagag caatgttatt gccttgatcg
    12661 ataggaatta aaaccccaaa aagattaaaa aaacaccaca aaacggatgt ttcttcaaca
    12721 ccacttttgc tcatatgaac ggaaccgacg attaaactgg atggctctga ttgattcagg
    12781 gtatgaatgg cggttttttg ctccgtttcc ctcaaaatgg acgcaacttt ccctctgcgg
    12841 ctctcagccg caccaccgca tccgggccag c
//
LOCUS       DAZSBF010000112        12836 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZSBF010000112 DAZSBF010000000
VERSION     DAZSBF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12836
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.113"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHXWD_002524"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHXWD_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR7621415.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHXWD_002525"
     CDS             433..621
                     /locus_tag="ACHXWD_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR7621416.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHXWD_002526"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHXWD_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR7621417.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHXWD_002527"
     CDS             816..989
                     /locus_tag="ACHXWD_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR7621418.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHXWD_002528"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHXWD_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHXWD_002529"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHXWD_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR7621419.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHXWD_002530"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHXWD_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR7621420.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHXWD_002531"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHXWD_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR7621421.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHXWD_002532"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHXWD_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR7621422.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHXWD_002533"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHXWD_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHXWD_002534"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHXWD_002534"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR7621423.1"
                     /translation="MFSRELDDEQLTQIASAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHXWD_002535"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHXWD_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR7621424.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHXWD_002536"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHXWD_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR7621425.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..>12836
                     /locus_tag="ACHXWD_002537"
     CDS             12531..>12836
                     /locus_tag="ACHXWD_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621426.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     "
BASE COUNT         3145 a         3248 c         3335 g         3108 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaatgata cacaaattta ttcaggggtt tcagga
//
LOCUS       DAZSBF010000113        12359 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZSBF010000113 DAZSBF010000000
VERSION     DAZSBF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.114"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHXWD_002538"
     CDS             complement(<1..330)
                     /locus_tag="ACHXWD_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR7621427.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHXWD_002539"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHXWD_002539"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR7621428.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHXWD_002540"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHXWD_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR7621429.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHXWD_002541"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHXWD_002541"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR7621430.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHXWD_002542"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHXWD_002542"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR7621431.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLMDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHXWD_002543"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHXWD_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR7621432.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHXWD_002544"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHXWD_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR7621433.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHXWD_002545"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHXWD_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR7621434.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHXWD_002546"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHXWD_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR7621435.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHXWD_002547"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHXWD_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR7621436.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHXWD_002548"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHXWD_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR7621437.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHXWD_002549"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHXWD_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR7621438.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHXWD_002550"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHXWD_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR7621439.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3056 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccatcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaact
//
LOCUS       DAZSBF010000114        12334 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZSBF010000114 DAZSBF010000000
VERSION     DAZSBF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.115"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(97..249)
                     /locus_tag="ACHXWD_002551"
     CDS             complement(97..249)
                     /locus_tag="ACHXWD_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7621440.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(514..1248)
                     /gene="cysH"
                     /locus_tag="ACHXWD_002552"
     CDS             complement(514..1248)
                     /gene="cysH"
                     /locus_tag="ACHXWD_002552"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR7621441.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1323..3035)
                     /gene="cysI"
                     /locus_tag="ACHXWD_002553"
     CDS             complement(1323..3035)
                     /gene="cysI"
                     /locus_tag="ACHXWD_002553"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR7621442.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3035..4834)
                     /gene="cysJ"
                     /locus_tag="ACHXWD_002554"
     CDS             complement(3035..4834)
                     /gene="cysJ"
                     /locus_tag="ACHXWD_002554"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR7621443.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5153..5515
                     /gene="queD"
                     /locus_tag="ACHXWD_002555"
     CDS             5153..5515
                     /gene="queD"
                     /locus_tag="ACHXWD_002555"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR7621444.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5593..6864
                     /locus_tag="ACHXWD_002556"
     CDS             5593..6864
                     /locus_tag="ACHXWD_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7621445.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6855..7115
                     /locus_tag="ACHXWD_002557"
     CDS             6855..7115
                     /locus_tag="ACHXWD_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR7621446.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7132..7707
                     /locus_tag="ACHXWD_002558"
     CDS             7132..7707
                     /locus_tag="ACHXWD_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR7621447.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7855..8715)
                     /locus_tag="ACHXWD_002559"
     CDS             complement(7855..8715)
                     /locus_tag="ACHXWD_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR7621448.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8712..9491)
                     /locus_tag="ACHXWD_002560"
     CDS             complement(8712..9491)
                     /locus_tag="ACHXWD_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR7621449.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9469..10878)
                     /locus_tag="ACHXWD_002561"
     CDS             complement(9469..10878)
                     /locus_tag="ACHXWD_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7621450.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10900..12333)
                     /locus_tag="ACHXWD_002562"
     CDS             complement(10900..12333)
                     /locus_tag="ACHXWD_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR7621451.1"
                     /translation="MVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHGIYTLPIP
                     AAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDGSAMNQII
                     NIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRSIGQFSTL
                     YGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITEVTVKIFK
                     FTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHFADGKCVL
                     IFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQIL
                     KTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYF
                     VYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSA
                     WALLEGLKKQFDPNGIMNTGTIYPIEK"
BASE COUNT         2825 a         3405 c         3151 g         2953 t
ORIGIN      
        1 aggcataata ttcacataaa taatataaat taaaaaaggg tgcaggagac caaaccatca
       61 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
      121 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcgcctac
      181 catcagcgtg aatcccagta ctgtaaaaca cagcactatg attgccacaa gggcatattt
      241 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      301 acgctggcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      361 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      421 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      481 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      541 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      601 atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
      661 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
      721 tacgccacgc tgaattgcca gcaccggtaa attggcgcga ctgccggatt gttcacggcg
      781 caggccagca aaccaggttt gcgcgttaag ttctttcaga gcccggttca tcggttcgac
      841 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgctcccaca gttttccgta
      901 gcgtgcttcc tgccaggctg cgctttcggt agcacggtac actttcaggt tgagcttgag
      961 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacaagtaac ccgtatcggt
     1021 gaggatcacc ggaatatccg ggcgaatttg attcaccaga tgcaggctca ccgccgcctg
     1081 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggctac
     1141 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tccgccagcg ccagaatacg
     1201 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1261 gtttgccttg cctgatgcga cgctcacgcg ttcttatcag gcctacaaga ccgggctgat
     1321 ggttaatccc acaaatcgcg cgccggatcg agcaccgggc gaatgatgcc cgcacgcacc
     1381 gtaaagtcgc cgaagccttc acccgcttcg cgctctttcg cccagcgccc tatcagttca
     1441 tcaagcgacg ccaggatttc cggctcggtg atgttttctt tatacatccg tgggatacgt
     1501 gtcccaatgc ggttgccgcc aagatgcagg ttgtagcgac ccggcgcttt acccaccagg
     1561 cccacttccg ccagcatcgc gcgaccacaa ccgttcgggc agcctgttac acgcatcacg
     1621 atatgctcat cgctgacacc atgtttcgcc attaaattat cgatgttgtc gataaaagac
     1681 ggcaggaaac gctctgcttc cgccatcgcc agcgggcaag tcgggaatga cacgcaggcc
     1741 atcgagtttt cacgctgcgg cgtgacggca ttcattaacc cgctctcttt ggcgatcttc
     1801 tcgatcttcg ctttctcgct ttccggtaca ccggtgatga tcagattctg gttcgccgta
     1861 atgcggaaat cgcctttgtg gatcttcgcg atctccagca ggccggtttt cagcggacgc
     1921 cccggataat caaggatgcg accattttcg ataaacagcg tcaggtgcca gttatcatca
     1981 atgcccttaa cccagccaat acgatcgcct cgtccggtga actcatatgg acggatcggt
     2041 tcaaatttga tccccgcgcg acgctccact tccgctttaa acgtctcaac ccccacgcgc
     2101 tccagcgtgt atttggtttt ggcattttta cgatcggttc ggttacccca gtcacgctga
     2161 gttgtcacga cggcttcggc caccgccagc gtatgctcca gcggcagata gccaaactcg
     2221 ctcgccgtgc gggcgtaggt tttcttgttg ccgtgttcga tagaaagccc accgcccacc
     2281 agcaggttga agcctaccag cttgccgttt tcggcgattg ccacgaagtt catgtcgttg
     2341 gcatgcagat cgatatcgtt ctgcggcggg atcactaccg tggttttgaa cttacgcggc
     2401 aggtaggtct gaccaaggat cggttcttca tcagtagtgg cgactttttc ctggtcgagc
     2461 cagatctccg catacgcgcg ggtacgaggc aacagatgct cagaaatctt cttcgcccac
     2521 tcgtacgctt ccgcgtgcag ctgcgactcg taagggttcg aggtgcagag tacgttacgg
     2581 ttcatgtcgt tagctgtcgc cagcgcatca agaccgaccg agtgcagcat ctggtgcacc
     2641 ggtttgacgt tctttttcag aatgccgtgg aactgaaacg tctggcggtt ggtcaggcga
     2701 atgctgccat agatggtgtt ttcaccggca aatttgtcga tcgcctgcca ctgtttagtg
     2761 gtaatcaccc cacccggcag acgacagcga agcagcatcg cgtggcgcgg ctccagcttc
     2821 tgttcagcac gttcggcacg gatgtcgcgg tcatcctgct gatacatgcc gtggaagcgg
     2881 atcagcagga agttgtcgcc cttaaagccg ccggtcagac cgtcgtttaa atcttccgca
     2941 atggtgccgc gcaggtagtt gctttcaagc ttcatgcgct cggcgtctgt cagttttcct
     3001 tcgaccacta aaggccctgg atgtttttcg ctcattagta gacatctcgc tgataacggc
     3061 gctctacgcg cagctcactt aaaaattcat ccgccgcttc ggtgtccatg ccaccaaatt
     3121 cggcaatcac ttccagaagt gcctgctcaa cgtctttcgc catgcgatta gcgtcgccgc
     3181 agacataaat gtgggcaccg tcattgatcc agcgccacag ctccgcgccc tgttcgcgca
     3241 gtttgtcttg tacataaact ttttcttttt ggtcgcgcga ccaggcaaga tcgatacgcg
     3301 tcagcacgcc ctctttgacg tagcgctgcc actccacctg gtagaggaaa tcttccgtaa
     3361 agtgcgggtt gccaaagaac agccagtttt tacccggcgc ttcgtcggcg gcgcgttgct
     3421 gcataaaggc acggaacggc gcgatgccgg taccggggcc aatcataatc actggcgttt
     3481 gcgggttagc gggcaggcgg aaattgtcgt tatgttcgat aaatacgcgg acttcgccct
     3541 cttcttccac gcgatccgcg aggaagctgg aggcaccacc ggcacgggcg cggccttcca
     3601 cgtcgtaacg caccacacca acggtgacgt gtacttcgtt ctcgacttcc gcctgcgagg
     3661 aggcgatgga atacaggcgc ggggtcagcg ggcgcagcag gttaatcagc gcttcggcat
     3721 ccagttgcgc cggagagaaa cgcaccatgt cgacaatcgg cgtggtcgcg gcgtaatgct
     3781 gtaacttcgc tttatcgccc accagcggca gcagcgtttc gctgcgggta agcgtggcat
     3841 agttctcaac aatgttggcg gtgttgacgg tcagttcgaa gtgccactgt agcgcttcgt
     3901 tcagaggcaa cgttttgccc tcgacggtga caggttcatc gcctttcagc cacagcagtt
     3961 cgacaagttc tttcaccagt gccgggtcgt tctgatacca gacacccagc gcgtcacccg
     4021 gctggtaacg caggcctgaa tcgcctaagt caatttcgat atgacgaacg tctttttcag
     4081 agttacgccc ggtaattttc tggttaaccg aaaggctcgc caccagcggc gcgtctttgc
     4141 tgtacgggct ggtgtggatt tcatttaccg cgccagtagc gacggattgc gaaggtgccg
     4201 cgacaggcgc acgcgattta agcgcatcaa ccacgcgggc gcgccactcg ctggcagcag
     4261 cctggtattc aacatcggca tcgacacggt cgagcaggcg ttcaccaccc agttccgcca
     4321 gcttgctgtc gaaatctttc ccggactggc agaaaaattc ataagagcta tcgccgaggc
     4381 taaacacggc aaacgcggtg ttttccagct ttggcgcttt tttggagaac aggaacttat
     4441 gcagcgcgac ggcttcttcc ggcggttccc cttccccttg cgttgacgtc actacgatga
     4501 gcagtttttc gctggcgatt tgtttgaatt tatagtcgcc cgcgttcacc agcttaacgt
     4561 tcagttttgc tgctaataaa tcatcgcgta atgcttcagc aacccggcgc gcattgccgg
     4621 tttgcgaggc ggagataata gttatacccg gcatttctgc ggctggcgct ggcgtcgctg
     4681 caagcgcagc aggctgctga ttgagtacgc cccagaaata gccagaaacc caggcaagct
     4741 gggtaggcgt taaatcggtc gtggccgcct gaaggcgtgc cagttgctcc gggttcaacg
     4801 gaagcaacgc ggaaggtggg acctgtgtcg tcatgcgtcg ttatgttcca gtaagcaaag
     4861 ctgtttctgc gccctgtcag cgcccataaa acagaagaga agataaggtt aacggggtga
     4921 acggtgtgga ttaaagacgg gatagcgata actaataacc aaatcgacta acctgtttta
     4981 gcaatagtct ttaacaacaa aatagattaa ccaacccaat gaaaaacaaa tgaatttagc
     5041 caatcattaa gataaatcag cgattttgcg caacaaagtc gttttagata atgcgaaaaa
     5101 acagcctttc cggtactcta cggcggtttt atcgtcctgt agagaaatta tgatgtccac
     5161 cacgttattt aaagatttca ccttcgaagc cgctcaccgc ttaccacacg tcccggaagg
     5221 gcataaatgt ggtcgcctgc acgggcattc ctttatggtg cgactggaaa ttaccgggga
     5281 agtcgatccg catacgggct ggattatcga tttcgctgaa ctgaaagcgg cgtttaagcc
     5341 aacttatgag cgcctcgatc accattatct caatgatatt ccaggtctgg aaaacccaac
     5401 cagcgaagtt ttagcaaaat ggatttggga tcaggttaaa cccgttgtgc cgctgttaag
     5461 tgcggtgatg gtaaaagaaa cctgcaccgc agggtgtatc tatcgcggcg aatgataaga
     5521 gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta gggtgttgtg ttctggatat
     5581 ctggggcatg acatggaaga cgactgcgac attattatta ttggtgccgg tattgcaggc
     5641 accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg ttttgttact ggaacgcgct
     5701 gaaatccccg gcagcaaaaa tctttccggc gggcggttat atacccatgc actcgcggaa
     5761 ctcctcccgc aatttcatct gaccgcgcct cttgaacgac gcatcactca cgaaagcctt
     5821 tccctgttaa cgcccgatgg cgtaacgacg ttttccagct tacagcccgg cggtgaatcc
     5881 tggagtgtat tacgtgcacg gttcgatccg tggctggttg ccgaagccga aaaagaaggt
     5941 gtcgaatgca tccccggagc gacggtggat gcactgtatg aagaaaacgg cagagtctgt
     6001 ggcgttattt gtggtgacga tattctccgc gcccgttatg tggtgctggc agaaggtgcc
     6061 aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc ctgctggcga agcgatggcg
     6121 ttggggatca aagaagtgct gtcgctggaa acatccgcta ttgaagaacg ttttcatctg
     6181 gagaataacg aaggcgcagc gttgctgttc agcggcggaa tctgtgatga cttacccggc
     6241 ggcgcatttc tttatactaa tcaacaaacg ctctcgttag ggattgtttg cccgctctct
     6301 tcccttacgc aaagtcgtgt tccggcaagc gagctgctga ctcgctttaa agcgcatccg
     6361 gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt atggtgcgca tctggagcca
     6421 gaaggtggct tgcacagtat gccggtgcaa tacgccggta acggctggct gctggtgggc
     6481 gatgcgttgc gcagttgcgt caataccgga atttccgtgc gcggcatgga tatggcgctg
     6541 actggcgcac aggcggcggc acaaacgcta ataagcgcct gccagcaccg cgagccgcaa
     6601 aatctgtttg cgctttatca tcacaacgta gagcgcagcc tgctatggga tgttctacaa
     6661 cgttatcagc atgttccggc gcttttgcaa cgccctggct ggtatcgggc gtggcctgcg
     6721 ttaatgcagg atatttcccg cgatttatgg gatcagggtg ataaacctgt tccaccgctg
     6781 cgccagttat tctggcgtca tttacgtcgt catggcctgt ggcatctggc gggcgatgtt
     6841 atcaggagtc tgcgatgtct gtagcccata atctctggcg cgttgctgat gcgccgcaca
     6901 ttgttccggc tgactccgtt gagcgtcaga tggtacaacg gttgattaac gcctgtccgg
     6961 caggtctttt ttcgctcaca ccggaaggtg acttacgcgt tgattatcac ggctgcctgg
     7021 agtgtggcac ctgccgtttg ctgtgcgacg aatccacact acaacagtgg cgctatccgc
     7081 cttccggatt cggcatcacc taccgctttg gataaataat aaggataatt tatgcccctc
     7141 ttacacctac tccgccagaa tccggtgatt gctgccgtta aagataatgc cagcctgcaa
     7201 ctggctatcg attctgaatg ccagtttatt tccgtgttgt acggcaatat ctgcaccatc
     7261 agtaatatcg ttaaaaagat taagaacgcc gggaaatatg cttttattca tgttgatttg
     7321 ctggaaggcg cgtcaaataa agaagtcgtt attcagtttt tgaaactggt gaccgaggcg
     7381 gacggcatta tcagtaccaa agcctcaatg ttgaaagcgg caagagcgga aggttttttc
     7441 tgtattcatc gcctgtttat tgttgattca atttcgtttc acaacattga taagcaagtt
     7501 gcgcaatcga atccggattg tattgagatc ctgccaggcg gtatgcccaa agtgctgggc
     7561 tgggtgacag agaaaatccg ccaaccgctg attgccggtg ggctggtgtg cgatgaagaa
     7621 gatgcgcgta atgcgattaa cgcgggtgtc gtggcgcttt ccaccacgaa taccggggtc
     7681 tggacgttag cgaaaaaatt actttgacgg gataatcgta accaattgaa tttggtttga
     7741 tttgtaggcc gcacgccaca tccgacattc agcgcctgat gcgacgcttg acgcgtctta
     7801 tcaggcctac aagtccccag gccagaaccg taggcaggat catccggcaa cacgttattg
     7861 gcaatctgcg tgaatgtttg tcaccagtgc ctccagcacc actttccagt catcaaccac
     7921 gccaacatct gcctgtgaaa ataccgcagc gctggcgtca tgattaatcg ccaccacaaa
     7981 cttgctattg cgtacgcccg ccattaacgc tgctgcgcca gaagcgccaa caacaataca
     8041 cacctcaggt gccagcagat gtccggaaat accaatcact ttttcagcat caacgccacc
     8101 gttcatcact cgcgcccgac tgtagcccac ttcggctccc agcttttctg ccagcatagc
     8161 gatttcctgg ttatctgcct ctccgccctg ccccaccacc agcacccgtc tggcctcagc
     8221 taatgggtcg cgagtaacat ttttcagatc ctctgtgcta accagccagt ccggaagcgc
     8281 acccgggaca atgttcagtt gttgcattcc agagggcaac gtggcgtttt tcgctgctcc
     8341 cgcctgacgc gccagggaaa ggcatagcgg gcgtttttct gtttgcagcg tcgccgttag
     8401 cgcatttccc cagtgggatt tgcgcacgct tacggttgga atatctaacg aagtcacctg
     8461 gcagatgctt gcaccatgta aacgccaggc cagccgggtc gacagttcat cgccaaacgt
     8521 tcccggcgga aagagaacaa cgtccgcagg cgttcgctgc cactgttcca ccaacgcatc
     8581 cagcacctgt tccgccacta caggctgagg ttcaatctgc cagtgcgcca gggtacaacc
     8641 gctgaaatcc tgcgccgcca gccagcttgc tattgcggca ttctcctgat ttattgtcac
     8701 aattgcgata ttcatggctg catcctttgg cgtaaatagt cctgccatag cttttgagct
     8761 ttttctgcta ccgtttgccc atcaatcagc gtcgctccgc gtcgttgctc tgctcttgcc
     8821 agttgcagac actgcatcgc tggcatttcg gcagcaacag ttttacggat aatttctgct
     8881 ttccccgcag ccattcgctg gcgcattccc ggtactggca aggccacttc accgcactga
     8941 cgcacggcaa tcaccgcagg caagcgaacc cggcaacaac gcaacccatg ttcagtacgc
     9001 tgttcgaggg tgataaacag cgcgtcgaga gtgaaacgct ccacctgggt gaagcagggc
     9061 cagcccagca tttccgccag caaaaatggc gtttgcccat tctgcccttc gctgctttgg
     9121 cagccggtaa ttatcagatc cagcggattc tgatgctgcc attcggcaat atggcgagcg
     9181 acaaattccg gcgcaaagcg cagatctgcc gccgtctcca gcaatacagc ttcctcaaac
     9241 ccaagagcca tgagatagcg cagccagtgc aatgcccgtt catcccccat gctcaacgcg
     9301 gttaaagaca tcggcgtacc gttttttctc tgcgccaaca gcagcgcggc agcggcctgt
     9361 tcatcagcac cgagtaaact tcgcagtagc gaaatatccg gtccgctttt accctgagcc
     9421 gccgcctgcc actctttttc cgccagcatt ccggcatccg gttcggcttt aaacgctaac
     9481 agaatgttca ttcgcacctc ctacatttcc cgccgccacc agcgggaggg ctttagtttc
     9541 tggtgcccat agccaggtca caacaaaatc aaccaacaac actgtcgcca ggagcaataa
     9601 ggtgacttgc attccccact gcgccagcac ccacggcagc aggccagtac ttaccgccgc
     9661 gccaagtcga ctcatggcgg tggcaaaacc gacgcccagc gagcgaatgt cagtaggaaa
     9721 actttccgca ggcaaaatgc ccaccagatt actgactgcc gaaatggtgg tgctgaagag
     9781 aacaaaaagc agcagcgtta atgaactgcc ggaaggcaaa caggccatca ctaccagcgt
     9841 tgccgccagc agcaaaaaac ttcccagcaa aaatttgcga tgtgccagca ggtgcgtcag
     9901 aactaatccc agcagcgcgc ccacaattaa caacgcatta agcatcaggc tggcagtcag
     9961 cgcatcttcc agaccaatag tctgggcgat agttggcagc caggtataaa tcacaaacca
    10021 tgggattacg aggcagacaa agaagacgct gttaaacgcc gtgcgccgcc agtaacgcga
    10081 agagaacaag gttttgatgt gtttatgggt cgccgttacc acttcatcgc ccagtaaaac
    10141 atggggacca aaatagcgat gcacgatagc gtgagcttct gcaaaacgcc cctggcgcag
    10201 tagccagcgt ggtgattctg gcgttcccca gcgtaataac gtaatcaaca acgcgggcag
    10261 agctgccgat gccagtagcc agcgccaggc ctccgggttt tcggaaataa agtgatgtcc
    10321 ggcaatactt gccagcacat agcctacggt ccacaccacg ctgaatgcgc ccagcaaaat
    10381 accgcgatgg cggcgcgggg aaaattcagc cagcaaggtg tgacctactg aataatcgcc
    10441 tcccagacca atgccaatca aaatgcgcag tccaataaga tgctctggcg tggtggcaaa
    10501 aaattgtaaa aacgaagcaa gcgtaatcag caaaaagctg aaggtgaaga ttttttgccg
    10561 accaatatgg tcggagatcc acccaagaac caggctgcca aggaacaaac caaggagcgc
    10621 cgagccgccg atcattcccg ccataaacgg cgtcagttgc atggcgggcg taagctgaat
    10681 aatggcgtaa ccaatgacgc cgagaacata accgtcggtc aggtgtgcgc cgaaagtgag
    10741 cgcagcaatg cggcagtgaa aacggttaag cggtaaatca tccattcgca ccggtgaagt
    10801 gttcatcttt accccttcat tgtccggtaa taagaacggc ttctcgcctg agaagccgaa
    10861 aaggcaacgc cccgtcatcg cggaacgctg cctgacacat tatttttcaa tcggatagat
    10921 agtacccgta ttcataatgc cattaggatc gaactgcttt ttcagccctt ccagcaacgc
    10981 ccacgcgctg ccgtgttcca gtttgctcca gtgaacgcga tgtttaccga taccgtggtg
    11041 gtgcaccatc gaaccgccga ggcgaatggt ttcttcacag atgatcttgt tgagcggatt
    11101 gtggtacttg tcgatttcct cttccggctt acagtcaacg acgttgtaat cgtagacgaa
    11161 gtacatgttg gtgccgttct gatagctatg agaggaatga ccgcccagca tggtgatgtc
    11221 gtcggcgtgc gggaactcag tacgaatacg gttaataacg ctttcgtaga tttcgtggat
    11281 gcagctccag cagccggaca cttcagtggt aaagcccatg ttgccggttt tgaggatctg
    11341 cacacgttcg gcagccactt tatccggtcc ccagttcagg ttgttaaacc aggtttcgat
    11401 cagcttgctg tccacgcgct ggcattgcgg gtagcgggca acgatttccg caatcccttc
    11461 gcccgtcgcc ttcgcaatgc gagggttacc ttcagccata aagatcagca cgcattttcc
    11521 gtctgcaaaa tgggtgaagt gttgggtgcc atcttcagcg tcatacaaac gagcgatcga
    11581 cggacgatac ccttccacca tgacttcacg caggatgttg aagtcggttt tcatgtcttc
    11641 caggatatag ccgtagaaga ggttgttttc cggggtgaat ttaaagattt tcactgttac
    11701 ttcagtgata tagcaaagtg caccttcgtt accgatgatg atgtggcgaa tgtccgggcc
    11761 agccgcgcgg cgtggcacgt ttttaatgcg tgtgacggtg ccatcagcca atactgcttc
    11821 cagaccaacg accatatctt cgattgcgcc gtagagtgtg gagaactgcc cgatactgcg
    11881 ggttgctacc aagccgccca tctgcgccag cggctttgac tgcggagaat gccccgtggt
    11941 gtaacctttt tcacgcaacg cattttccag cacctccagc ggaaccccac attgcgccgt
    12001 cgcctgcata ttctcaatat caatattaat gatttgattc atggcggagc cgtcaagcac
    12061 caccgagttt tccacaacgg tttccagccc accttcagtg gcggaagcgc cggtacgcgg
    12121 cacaccgtta attttgtgcg cattcataaa attcagcaca cgggatactt gctctgtgga
    12181 accgagtttt acgaccgctg ccggaatcgg caaagtataa atgccatgaa tatccggaaa
    12241 tttacgaaaa cggtcaatac tgttcttctt taatactgtt tcatcggtaa ttacgcgatc
    12301 tgcaccaaca atttccttta gttggtcgac aatc
//
LOCUS       DAZSBF010000115        12048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZSBF010000115 DAZSBF010000000
VERSION     DAZSBF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.116"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            203..973
                     /locus_tag="ACHXWD_002563"
     CDS             203..973
                     /locus_tag="ACHXWD_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR7621452.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            964..1437
                     /locus_tag="ACHXWD_002564"
     CDS             964..1437
                     /locus_tag="ACHXWD_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR7621453.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1545..2084
                     /gene="dnaT"
                     /locus_tag="ACHXWD_002565"
     CDS             1545..2084
                     /gene="dnaT"
                     /locus_tag="ACHXWD_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR7621454.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2087..2824
                     /gene="dnaC"
                     /locus_tag="ACHXWD_002566"
     CDS             2087..2824
                     /gene="dnaC"
                     /locus_tag="ACHXWD_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR7621455.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2873..3367
                     /gene="yjjA"
                     /locus_tag="ACHXWD_002567"
     CDS             2873..3367
                     /gene="yjjA"
                     /locus_tag="ACHXWD_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR7621456.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3621..5912
                     /gene="opgB"
                     /locus_tag="ACHXWD_002568"
     CDS             3621..5912
                     /gene="opgB"
                     /locus_tag="ACHXWD_002568"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR7621457.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6051..7073)
                     /gene="lgoD"
                     /locus_tag="ACHXWD_002569"
     CDS             complement(6051..7073)
                     /gene="lgoD"
                     /locus_tag="ACHXWD_002569"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR7621458.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7212..8126
                     /locus_tag="ACHXWD_002570"
     CDS             7212..8126
                     /locus_tag="ACHXWD_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR7621459.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8341..9692
                     /locus_tag="ACHXWD_002571"
                     /pseudo
     CDS             8341..9692
                     /locus_tag="ACHXWD_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9741..11396)
                     /gene="tsr"
                     /locus_tag="ACHXWD_002572"
                     /pseudo
     CDS             complement(9741..11396)
                     /gene="tsr"
                     /locus_tag="ACHXWD_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11515..11961)
                     /gene="hpaR"
                     /locus_tag="ACHXWD_002573"
     CDS             complement(11515..11961)
                     /gene="hpaR"
                     /locus_tag="ACHXWD_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR7621460.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2918 a         3193 c         2947 g         2990 t
ORIGIN      
        1 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
       61 ttatcaccga gcttgtcccg ataaccagga ttaatcagcg caattttgat cttaacctac
      121 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      181 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cacggctttg
      241 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      301 ttcctcacga ctgggtcggg cgttggggat ggacagcgtc gaaagttcta tctcttcgaa
      361 cgccatagtg ctgacaacca ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      421 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttctggcgga
      481 gcaccatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc agccattacg
      541 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgcaaact
      601 caataaaggt ggctgggatg gtgccgtcat caccttcttt gccagtacgg ccgcgatgta
      661 tatccgccag ctgctggcac aacgtcatct tcatccgcag atcaactttt gcctcaccgc
      721 tttcgccgcc accaccattt ccggattgct tttgcaactt cccactttca gcaatacacc
      781 cactatcgcg atggccgcca gcgttctgct gctggtgccg ggatttccgt tgattaatgc
      841 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
      901 tctgctgaca ctggctacct gcgtcggcgt ggtgatggca ctgacgattt gggggctacg
      961 cggatgggtg tgatcgagtt tctgtttgca ttggcgcagg atatgatcct cgccgccatt
     1021 ccagcggtcg gctttgcgat ggtgttcaac gttcccgttc gggcattacg ctggtgtgcg
     1081 ctgcttggcg cgattggtca tggttcacga atgatcttaa tgaccagcgg gctgaatatt
     1141 gagtggtcaa cctttatggc ctccatgctg gtcggcacca ttggtattca atggtcgcgc
     1201 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1261 atatcggctt ataccgcaat gatttcggcg gtaaaaatca gccagttagg ttacagcgaa
     1321 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1381 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1441 tgcctcgctt aaagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1501 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1561 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1621 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     1681 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctggcgcgt ccgggaagcg
     1741 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     1801 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     1861 cgctatgggg tgtggcacta agagagccgg tgaccaccga agaactggcc tcattcattg
     1921 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     1981 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2041 tcagcgaacc tgacagtcaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2101 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2161 gggtgaagaa ctactggcct ggcagaaaga acaaggagcg atccgctccg ccgctctcga
     2221 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcggccact
     2281 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2341 aagcaaagcg cgccagtacg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2401 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2461 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2521 cttcaggaat agcggtacca gtgaagaaca actgcttaac gatctgagca acgttgatct
     2581 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     2641 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     2701 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     2761 cagtttgtgg gtgatcttca actgggatag ctaccgtagc cgggtaacag gtaaagagta
     2821 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     2881 aactgttaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     2941 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3001 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3061 gaaccaggcc ttaagcgcag ataacatgaa caacgccgca ggcattctgc aatactgcgc
     3121 gaagcaaaag ctggcgtcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3181 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggatg gcattcaggg
     3241 tttgctgaaa accaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3301 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3361 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3421 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3481 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3541 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3601 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     3661 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acattaacgg
     3721 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     3781 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     3841 gcaaatacat cctgccgggt atcggcattg tgctggggct gacagcggtg ttcggtgcgc
     3901 tgggctggat cctgcgccgt cgtcgccatc atccgcacca ttttggttac agcctgctgg
     3961 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4021 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4081 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4141 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4201 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4261 gcatggtggc ttctcagtgc ggcataccgc tgtttgcccc ctttgaaggc aacgcctccg
     4321 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4381 gttatcagaa ctatttcgtg cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc
     4441 tgaagtcgca tggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtggccg
     4501 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4561 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4621 atacccatca cccggatggt tttatctcgc gtacctgtaa ccgcaaaaaa tatgattttg
     4681 acggtaagcc gaaccagtca ttcagcgcgg taagttgcag ccaggagaat atcgcggcgt
     4741 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg
     4801 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     4861 tgttttttgt cattcgtggc gacaagccgc agcaagagac gctggcagtg aagcgtaaca
     4921 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     4981 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5041 cattggcgtg gaagccggat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5101 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5161 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5221 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt
     5281 gctataagat ggcacaactc tgggctccgg aactggcact ctccaccgac tggtgtgtct
     5341 cgcaagggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggcagg
     5401 gcaaaacggc atttaaagat acggtcatcg acatggcgcg ttataaaggc aatgtcgata
     5461 cgctgaaaat tgttgataac gatattcgct acaaagccga cagtttcatc ttcaacgtcg
     5521 ccggtgcgcc agaagaggtg aaacagttta gtgggatttc ccgtccagag tcgtggggcc
     5581 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccaa
     5641 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     5701 cggtacgcgt aggcaatgaa gaacaaaccc ttgtgctggg caatgaagtg accaccacca
     5761 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     5821 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     5881 tggaaattaa agtggtggaa cgtgaaggat aattcctgac tgatgtgtag gtcgcataag
     5941 gtgcgaccta cacaccttct gcggcctaca aaacatcatt gagaaacaga aaaaaacagc
     6001 gcccaacact tccccggacg ctgcatggca actttaataa tctcgcctta tcagaaagta
     6061 attacaccct taatcaactc acgattgtta atcacatcgc gctcgtaggt ttctgccagc
     6121 gtggcgaacg gatagcgatg ggttaacatc atgtcagcag tgatttttcc ttccgccatc
     6181 agtcgaccga ctttagcaaa atcttccggc gtggcgttgc ggctacccat catcgtcgtt
     6241 tcttttttat ggaattccgg atcggagaac tgcaactcac ctttaaacag gccgacaaat
     6301 accaccgtgc cgccgtgacg aatcaaattc acggtgttat tcatcgcatg ttgattacct
     6361 gtcgcgtcga tcactttctg cgccagcgaa ccaccaaact gcgcccgtag ctgcgcgtca
     6421 aaatcttcgg ctgacgggtc cagtaaaggt aattccagac gcgttgccac atgttcacgg
     6481 cgcgccggac tggtatccgc caccaccacc tgtgcgccat cggctttagc gattgccgcc
     6541 gcgcccagac cgattggccc cgccccgacc accagcacct gctcgccggg agcaatggct
     6601 gcgcgacgca ccgcatgagc gctaatagcg aaaggttcaa tcaatgctgc cgcctgcggg
     6661 tcaataccgt ctgcgggcaa aatgttcgcc accggcacgc tcaaatactc actaaaaccg
     6721 ccatcctgat gcacgccaat gactgaaatt ttttcacagc aattggtacg cccgctttta
     6781 cacgccgggc attgctgaca ggcaacataa gggatcacag caacttgctg accattttta
     6841 agatcagcaa tatttttacc cagcccaaca atctccccac atatttcatg gcctaaaaca
     6901 cgtggataac taaaaaatgg ttgatttcca ccccaggcat gaatatcggt accgcaaatc
     6961 ccgacagact taatttttat taatgcttca ttgtcacccg gaataggtat ctcgcgttgt
     7021 ttccagacta attctttcgg ctgctggcaa attaaaacat tcatcgtaga catgattcgg
     7081 ctctccattt tttgttgcct tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa
     7141 atctgttttg tgaaaacaca cgcataaaaa tgttttaaat cgggttttaa tggactcaca
     7201 aggaggaaaa tatgagtcgt tcacaaaatt tacgccacaa tgtgattaac caggtcattg
     7261 atgatatggc gcggggtcat atcccttccc cacttccatc gcaaagtgcg ctggcggaaa
     7321 tgtacaacat cagccgcacc acagtgcgtc atattctcag ccacttacgc gaatgcggcg
     7381 tcttgacgca ggttggaaac gactatgtta ttgcgcgtaa acctgaccat gatgacggtt
     7441 tcgcctgtac taccgcttca atgagtgaac aaaacaaagt gtttgagcag gcttttttca
     7501 cgatgataaa ccagcgccag ttacgcccag gggaaacgtt ttctgaactg caactggctc
     7561 gggccgcggg tgtcagcccg gttgtagtaa gagaatatct tttaaaattc ggccgttata
     7621 atcttattca cagcgaaaag cgcggccagt ggagcatgaa gcaattcgat caatcctacg
     7681 ccgagcagct gtttgagcta cgcgaaatgc tcgaaacaca ttcacttcag cactttctca
     7741 acctgcctga tcatgatccc cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc
     7801 tactgcgtga caacatcggc aatagctttc gcatgttctc gcaacttgac agagattttc
     7861 actccttact gctttcagct gccgacaata tcttttttga tcaatcgctt gagatcatct
     7921 cagtcatctt tcattttcac tatcaatggg acgaaagcga tcttaaacag cgcaatatca
     7981 ttgccgtcga tgagcatatg accattctca gcgctctgat ttgccgcagt gaccttgatg
     8041 ccacccttgc gctccgtaac catttgaatt cagctaaaca atcaatgatt cgatccatta
     8101 atgagaacac gcgttatgct cattaagtac ggattaaaaa cagcaaacta ttagcgggtg
     8161 cacggtttta tcaaaagata aataccggga aattcccgct tacctatgct cacaatcagt
     8221 cgactaaaaa tgtttttgtg agcggtagta aagtcctaaa actttaacct gccgttaatg
     8281 tcaaccttgc gtaattcaca attacgtaaa caattaaaat tataaataca ggagtccggc
     8341 gtggaaaaag aaaatatcac cctcgatccg cgttcttcat ttactccatc ttcgtcggca
     8401 gatattcccg tgccaccaga tggattagtt caacgcagta cccgaattaa acgcattcaa
     8461 accaccgcca tgttgttatt attttttgcg gcggtaatca attatctcga ccgcagttcg
     8521 ctgtcggtag caaatttaac gattcgtgaa gaattgggat taagtgccac cgaaatcggc
     8581 gctttgctct ccgtgttttc actcgcttac gggattgcgc aacttccttg cggcccacta
     8641 ttggatcgta aaggcccacg cctgatgctg ggactgggga tgttcttctg gtcactgttc
     8701 caggcaatgt ctggcatggt gcacaacttt acgcagttcg tgttggtgcg tatcggtatg
     8761 gggattggtg aagcgccgat gaacccatgc ggtgtaaaag tcattaacga ctggttcaac
     8821 atcaaagagc gcggacgccc gatgggcttc ttcaacgcag cttctaccat tggcgttgcc
     8881 gtaagcccac cgattctggc ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt
     8941 accattggtg tactggggat ttttctcgcc atcggctggt atatgctcta tcgcaaccgc
     9001 gagcacgtag aactgactgc cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc
     9061 cgccgagatc cgctcagttt tgccgaatgg cgcagcctgt tccgtaaccg tacaatgtgg
     9121 ggaatgatgc tcggattcag tggcatcaac tacactgcgt ggctgtatct ggcctggctt
     9181 cctggttacc tgcaaacagc ctataacctg gatttaaaaa gcacagggtt gatggcggct
     9241 atccctttcc tgtttggggc tgccgggatg ctggtcaacg gttacgttac tgactggctg
     9301 gtcaaagggg gaatggctcc gattaaaagc cgtaagatct gcattattgc cgggatgttc
     9361 tgttctgccg cctttacgct ggtcgtaccg caagcgacaa catccatgac agcggttctg
     9421 ctgattggca tggcactgtt ctgtattcaa catcctgctg gggcttgatc cacgtcgcag
     9481 ttgcttctcg catgactgcg tcggtgggca gtatccagaa ctttgccagc ttcatctgcg
     9541 cctcttttgc gccgatcatt actggtttta ttgttgatac cacccactca ttccgtctgg
     9601 cactaatcat ctgcggttgc gtcaccgcag cgggggcact ggcgtacatc ttcctggttc
     9661 gtcagccgat caacgaccca cgtaaagatt aatctcctta tgcccgataa cattttgctt
     9721 atcgggcatt ttcatggcga ttaaaatgtt tcccagttct cctcgctatc tgccacggcc
     9781 attttacgcg gcgcagctgg cgtcacggtt tttaccacag ccgatgtttc acgctgctgt
     9841 tgctgaatcc ggaacactgc cactgcttcg gtcaggcgac tggcctgctc ttccagcgcg
     9901 gcggcggcag cggcagactc ttccaccagc gcggcgttct gttgagttac ccggtccatc
     9961 tcagcaaccg ctaagccaac ctgatcgata ccacggctct gctcatcaga agcagaagca
    10021 atttcgccca taatgtccgt cacgcgggtc acggcgctga caatctccgc cattgtttcc
    10081 ccggcgcttt cgaccagcgt agagccaaca tccactttcc ccaccgagtc ttcaatcagg
    10141 cttttaattt cacgagccgc ctgggcgctg cgctgggcca gattacgcac ttctcccgcg
    10201 accaccgcaa aaccgcgccc ttgctcaccc gcacgcgcag cctcaaccgc cgcgttcaaa
    10261 gccagaatat tggtctggaa ggcaatgccg tcaattacgc tgataatatc ggcgattttc
    10321 tgcgaactgg tggagatatc gcgcatagtc tgcaccacgt tatctaccac tttgccgccg
    10381 cgttgcgccg tttcagaagc acttaacgcc agatggctgg cctggcgcgc attttcggcg
    10441 ttctgcttca ccgttgcggt cagttactcc atgctggctg ccgtctcttc cagcgaagcg
    10501 gcctgttgct cggtgcgcga agagagatcg ttattgccgg tggcgatttc gctggcaccg
    10561 ctatagatgg cattggcccc gttgcgcaca tcaccgacgg tacgcatcag ctctccctgc
    10621 atatggcgca aactctctgc cagttgcccc atctcattag agccatccac ctcaatcggt
    10681 ttcaccagat cgccgcctgc aatatgacga atgctgtcaa tcaggcgatt cattggcgct
    10741 accagcgagg ctttaatacc gaaccagacg gcgaagatga ccgccagtac gacgatcatc
    10801 acgcccacca gaatccacat cgcctggctg taggaggcat tgttatcgct gacggcgata
    10861 tcatagagcc gatcgttttg ctccatgtaa gccacatact gcttctcgaa accgtcctga
    10921 tatccctggg tcggctgatc aaagaactcg ttgattttgc ctgcacctaa cagttggatc
    10981 agctccgcca gcgcattgtg ataaatatcg taattacgtt tgatctctgc cgctgcggcg
    11041 gtgctctgac gcgggtcacg cggcaacgct tcgtaatccg cccagttttt ttccgcctgt
    11101 ttcagcgaaa tactggcact ctgcatcagc tcagcaacgg ttgaaccgct accaatattg
    11161 ttctgatcca tcatgtagcg gatacccgcg cggttgaggg tgttacgcgt ctgcaacaac
    11221 gcgacccagc tgccattcag cgtggattgc tgctggcgaa tggtttgtaa aacagtgaaa
    11281 ttttctttgt cattctttaa ggcattaaag aacagaccgc ctgatgtcag ttgtaaaagg
    11341 ccaaaaacgg ccaaaaccag cagtaagctg gtcacgatct tgatacgttt taacatggtt
    11401 tctctttcct gtggaccgat gcaagatttt cggcctggaa agcaaaaact ttatgaaaag
    11461 gtgtaaccca ccatccatgg aaaaaccagg cgctggttat cagatactaa aaagttattc
    11521 attatcgccc gggatggctt cttgtcggga attgcccaga gcaataaatt cctctaacag
    11581 atgggttaac tgttgcattt tctcggcagt aaactgagct tcaatctgcc gataggcttc
    11641 ttcaatctgt gtctgagcgc ggttatacag cgcctgcccc tctttggtta gcgagatata
    11701 gagcttccgc tgatcattga tcggctttaa tcgcaacact aaaccgtcgc gttccatccg
    11761 cgtgaggatc ccggtcaggc ttgggcgcaa aatgcaggcg cgatacgcca gatcgtgaaa
    11821 atccattgat gggctttccg ccagaatacg cacgatgcgc cactgttgct cagtcaaatt
    11881 atgccgttta acgattgggc gaaaataact catcgccgct tctcgcgcct ggagcaacgc
    11941 aatggttagt gagtcgtgca ttatctttcc cctgaaaaat tcaaaatcat taatatagaa
    12001 acagttaatc gtcacgttac cgggactgcg ctgcccctgc aattcctg
//
LOCUS       DAZSBF010000116        12037 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZSBF010000116 DAZSBF010000000
VERSION     DAZSBF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.117"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..323
                     /locus_tag="ACHXWD_002574"
     CDS             <1..323
                     /locus_tag="ACHXWD_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621461.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHXWD_002575"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="ACHXWD_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHXWD_002576"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="ACHXWD_002576"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR7621462.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="ACHXWD_002577"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="ACHXWD_002577"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR7621463.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHXWD_002578"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="ACHXWD_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR7621464.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHXWD_002579"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="ACHXWD_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR7621465.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHXWD_002580"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="ACHXWD_002580"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR7621466.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="ACHXWD_002581"
     CDS             8424..8927
                     /locus_tag="ACHXWD_002581"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR7621467.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="ACHXWD_002582"
     CDS             9004..9687
                     /locus_tag="ACHXWD_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR7621468.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="ACHXWD_002583"
     CDS             9766..10752
                     /locus_tag="ACHXWD_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR7621469.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="ACHXWD_002584"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="ACHXWD_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR7621470.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2862 a         3094 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtacccg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactcat
//
LOCUS       DAZSBF010000117        11890 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZSBF010000117 DAZSBF010000000
VERSION     DAZSBF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11890
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.118"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            34..663
                     /locus_tag="ACHXWD_002585"
     CDS             34..663
                     /locus_tag="ACHXWD_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR7621471.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHXWD_002586"
     CDS             complement(664..1824)
                     /gene="ybdL"
                     /locus_tag="ACHXWD_002586"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR7621472.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHXWD_002587"
     CDS             1933..3021
                     /gene="hcxA"
                     /locus_tag="ACHXWD_002587"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR7621473.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(3031..3228)
                     /locus_tag="ACHXWD_002588"
     CDS             complement(3031..3228)
                     /locus_tag="ACHXWD_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR7621474.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHXWD_002589"
     CDS             complement(3410..5515)
                     /gene="cstA"
                     /locus_tag="ACHXWD_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR7621475.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHXWD_002590"
     CDS             complement(5696..6109)
                     /gene="entH"
                     /locus_tag="ACHXWD_002590"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR7621476.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHXWD_002591"
     CDS             complement(6112..6858)
                     /gene="entA"
                     /locus_tag="ACHXWD_002591"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR7621477.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHXWD_002592"
     CDS             complement(6858..7715)
                     /gene="entB"
                     /locus_tag="ACHXWD_002592"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR7621478.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHXWD_002593"
     CDS             complement(7729..9339)
                     /gene="entE"
                     /locus_tag="ACHXWD_002593"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR7621479.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHXWD_002594"
     CDS             complement(9349..10524)
                     /gene="entC"
                     /locus_tag="ACHXWD_002594"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR7621480.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            10899..11864
                     /gene="fepB"
                     /locus_tag="ACHXWD_002595"
     CDS             10899..11864
                     /gene="fepB"
                     /locus_tag="ACHXWD_002595"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR7621481.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
BASE COUNT         2855 a         3436 c         3056 g         2543 t
ORIGIN      
        1 ttataacgaa cggataaaag gaaaacgtca ggaatggggg atactatgca acaacgatta
       61 acgcaggatt taacgcaatt tctcgccagc ttgccggaag atgatcgcat caaagcgatt
      121 aatgaaatcc gcatggcgat ccatcaggtt agcccctttc gtgaagagcc agtggattgc
      181 gttctgtggg ttaaaaacag ccaacttatg cctaatgatt acaacccaaa caacgtggca
      241 ccacccgaaa aaaaactgct gcaaaaatcc attgaaatag atggttttac ccaacccatt
      301 gtggtcacac atacggataa aaatgccatg gaaatcgtgg atggttttca ccgccatgaa
      361 attggtaaag gctcatcatc attaaaatta cgcttaaaag gttatttgcc ggtgacctgt
      421 ctggaaggta cgcgtaatca acgcatcgcc gcgactattc gtcataatcg cgctcgtgga
      481 cgccatcaaa tcaccgccat gtcggaaatc gtccgtgaac ttagccagtt gggatgggac
      541 gataataaaa ttggcaaaga gctgggaatg gacagcgacg aagtgctgcg cctgaagcaa
      601 attaacggcc tgcaagagct gtttgccgac cgtcaatatt cccgcgcctg gacggttaaa
      661 taactaaagc tggcgcaggc gttcagctgc tgccagcaac gtcgattcct tcttggcaaa
      721 acagagacga atcagtttat gtgggaaggg atcggcgcaa aacaccgaca gcggaatcgc
      781 cgctacgccg tgctcctgcg tcagccactg gcaaaactca acatcatcca gggtagaaac
      841 cgcgctgtaa tccaccagca aaaagtatgt accttcacac ggtaaaatct ccagccggct
      901 ttcatttaag gcatttacca gaatatcgcg cttctggcga taaaagtccg gtaacgcaag
      961 ataatgctca ggttctgcac gtagcatatc agcaagcgcc agctgtgccg gggtattcac
     1021 cgaaaaggtc agatactgat gtaccttgcg aatttcggcg ctgatgggcg ctggcgcaac
     1081 acaataaccc actttccagc cggtcatatg ataggtcttg ccaaatgaag aaaccgccac
     1141 tgcccgctca cgcagctgcg aatgcgccag cacactggca tggccctgtt gtgaaaagtt
     1201 gatgtgctcg tagacttcat cgctaatgac aaaaatctcg tgcccggcga tcgcctgcca
     1261 caaagcggcg aaatcagcct gctgccagac agttgcactg gagttatgcg gagtgttgag
     1321 gatcaccagt ctggtgcgct cgcttaataa tgcggcaaat tcctgccagt caacgcgaaa
     1381 atgcggtggt tgcagtgcca tacgcttcac tattccccca gaaagcgcga tggcgggggc
     1441 gtaactgtca tagctgggat caaaacaaat cacttcatcg ccattgcgca ccagtgcggt
     1501 aatcgccgcg tataacgctt ccgtcgcccc tgccgttacg gtgatatcgc tatcggcatc
     1561 tggttgatag ccatacaaac gttccgtttt ctgagcaatc gcctcgcgca aggcctgcac
     1621 gccggtcatg ggcgcgtatt ggtttgcccc ctgggcaacg tggtacgcca gccgctcctg
     1681 taaatagcgc ggaccatcaa aatcaggaaa gccttgcgac aggttaatcg cctggtgttg
     1741 ctgcgccagc gcgctcatct gggtgaaaat agtggtgcca agttgtggaa gtttgctttg
     1801 tggaatcaga gggttatttg tcatttttat tgtacctgac gaagttgcgt gttgaaggca
     1861 ctataacacg atgctagtat ttggcaatca agacgtttag atgtctaaat aaaacaataa
     1921 ggacaacaca acatgcctca caatcctatc cgcgtggtcg tcggcccggc taactacttt
     1981 tcacatccag gaagtttcaa tcacctgaac gattttttca ctgatgaaca actttctcgc
     2041 gcggtgtgga tctacggcaa acgcgccatt gctgcggcgc aaaccaaact tccgccagcg
     2101 tttggacttc caggggcaaa gcatattttg tttcgtggtc attgcagcga aagcgatgta
     2161 cagcaactgg cggctgagtc cggtgacgat cgcagcgtgg tgattggcgt cggcggcggc
     2221 gcactgctcg acaccgcgaa agccctcgcc cgccgtctcg gtctgccatt tgttgccgtt
     2281 ccgacgatcg ccgctacctg cgccgcctgg acaccgctct ccgtctggta taacgatgcc
     2341 ggacaggcgc tgcattatga gattttcgac gacgccaatt ttatggtgct ggtggaacca
     2401 gagattatcc tcaacgcgcc gcaacaatat ctgctggcgg ggatcggtga cacgctggcg
     2461 aaatggtatg aagcggtggt gctggctccg caaccagaaa cgttgccgct aaccgtgcga
     2521 ctggggatca ataatgcgca ggccattcgc gacgtattgt taaacagtag cgaacaggcg
     2581 ctgagcgatc agcaaaatca acagttaacg caatcatttt gcgatgtggt ggatgctatt
     2641 attgctggtg gtgggatggt cggtgatctg ggcgatcgtt ttacgcgtgt ggcggcggct
     2701 catgccgtgc ataacggtct gactgtcctg ccgcaaaccg agaaatttct ccacggcacc
     2761 aaagtcgctt atggcattct ggtacaaagc gccttgctag gtcaggatga tgtgctggcg
     2821 caattaaccg gggcgtatca gcgttttcat ctgccgacca cgctggcgga gctggaaatg
     2881 gatatcaata atcatgcgga gatcgacaaa gtgattaccc acaccctgcg tccggtggaa
     2941 tacattcatt acctgccagt cacgctgaca ccagatacgt tgcgtgcagc gttcgaaaaa
     3001 gtggaatcgt ttaaagcctg acgtgcaaat ttagcagcag cgcgcgccgc cttttccacc
     3061 gtagcgcgcg tcctgccgct cacggaaaaa ctcttcgtag gtcatcggcg tttgatcggg
     3121 atggttaacc cgcatatgtt cgacatagtt gtcgtaatca ggcataccaa tcatcagctt
     3181 cgccgcctga cctaaatatt ttccggcttt tgccagtgaa tcaaacatct tggcctccgc
     3241 taaccatgtt gctgaaagag tgccggatgc ggcgtgaacg ccttatccgg cctacaaatg
     3301 ctatcaacgc gctcggtcag tcccctcgcc ccatcgggga gagggttagg gtgaggagga
     3361 accctcggca ctatgccaac attcgccaac atcccccctc actctgactt tagtgtgcgc
     3421 cttttgcctg cgccacgatc tcctcgacat tttccggcat tggctcatac ggcgtttctt
     3481 tcgccgttgg cttcggatct ttcaatgccg ccagtgccga cttaatcgag aacagcgcca
     3541 gaaccaccac gaccaccata aagaagatgg ttaacccggc atccagacgg ttgttgaaca
     3601 ccagttgcgc cagctgtgac tcagtatact gcgacggaat attgccgctg tctatcattg
     3661 cctggaactt attagcaatg gccaggaagc cgactttcgc atccgggcta aacgctttct
     3721 gccagcctgc ggtcagggta caaatcagca gccaggccgt tggtaccagc gccacccagg
     3781 cgtaacgttg acgtttcatc ttgaacaaca ccacggcaca gagcatcagc gccatccctg
     3841 ccagcatctg gttggcaata ccaaacagcg gccacagagt gttaatgccg cccagcggat
     3901 cgaccacgcc ctgatggagg aagtagcccc acgccagtac gcacagcgct gttgccagca
     3961 ggttagcagg cagtgaatcg gtccgcttca ggccaggaga caccacgccc agcagatcct
     4021 gcaacataaa gcgcgcagca cgcgtacccg catctaccgc cgtcagaata aacagagctt
     4081 caaacagaat ggcgaagtga taccagaacg ccacatccat cataccaccc agcgcaccgt
     4141 gcaggatgta agccatcccc accgccagcg tcggcgcacc gcccgcacgg gaaatgatcg
     4201 actgttcacc cacttcgctg gcaatctggt tgagcgtatc cggagtaatc gcaaagcccc
     4261 agctactcac cacctgcgcg gcagaagcga ccacatccgc cgtccctgcc ggagccagca
     4321 ccgccatcgg gctgttcatg gcgaagtaca cgcccggatc gatgatacag gcggaaacca
     4381 gtgccataat cgccacgaag gattccatta acattccacc gtagccgata aagcacgcct
     4441 gcccttcgtt cgccagcatc ttcggcgtgg tgccggaaga gatcagcgca tggaagcctg
     4501 acaccgcgcc acaggcgatg gtgataaaca ggaacgggaa cagattaccg gtccataccg
     4561 ggccagtgcc atcgacaaat ttggtcagcg caggcatggt cagcgtcgga cgcataatca
     4621 aaatgcctac cgccagacca acgatagtcc cgattttcag gaaggtagag aggtagtcac
     4681 gcggggccag cagtaaccac accggcagca ccgccgccac aaaaccgtag cccaccagca
     4741 tccaggtcag ctgcacgccg gtaaagtcaa agtacggtgc ccaggtcggg ctttctgcca
     4801 cccagccgcc agagataatg gcaaaaataa ggaacaccag gccaatcact gacacttcgc
     4861 caatacgccc cggacgcaga tagcgcaggt agattcccat aaaaatcgcc agcggaatgg
     4921 tgaacgcaac ggtatacgtt ccccacgggc tatgagtcag ggctttcacc acgatcatcg
     4981 ccagtactgc aaggataatg accatgatca taaagcaggc taccagcgca atcaccccgg
     5041 cagttggccc catctcttct ttgaccagct cacccagcga gcgaccgtca cggcgcgtag
     5101 aaacaaacag caccatgaaa tcctgcaccg caccggcgag aaccaccccg gcgagcagcc
     5161 agatcatccc cggcaggtag cccatttgcg ctgccagcac cggccccacc agcggccctg
     5221 ctccggcaat ggccgcaaaa tggtgaccga acagcacttt cttgtccgtc ggcacatagt
     5281 ccagcccgtc gttatggcgc accgctggcg tcatacgcgt cgggtcaacc gccagcacat
     5341 ttttggcgat atacagccca taaaaacggt aagcgatcag atagatacag accgacgcca
     5401 ccacaatcca cagcgcgttg atctgttccc cacgatttaa agcaatgtat cccagagcaa
     5461 atgctcccat tacagagagc actgtccaga cgaggtattt ccctgatttg ttcatagttg
     5521 ttatccgtgt gcgtgtccat agagatgtta cattttgttt ctataacatc tttgtacgat
     5581 ttaacaacac ggtaaccatg taaatcggag ttgaaaccaa tatttaactt aacaatgtta
     5641 actcgatcac tccgttaacc gagttttcct gcaatctcat ttaattctgt cgggctcatc
     5701 ccaaaactgc cgtacccagc cgacaagtgc agcaacgccg cccctgttca tcgaaaacga
     5761 tgatttccca gctctgattt tgccgcccaa gatgcagcgg ctggcagacg ccgcgtacct
     5821 tgccttcaga catcgggcga tgatgggtgg cgttaagttc cgtacccacc acacactgcc
     5881 cgtcacgggt catcataaat ccggccatcg atcccagcgt ttcagccagc gccgccgacg
     5941 cgccgccatg cagcaggcca aacggctgat gagtacgggt atcaaccggc atttcggctt
     6001 ccagcacatc atctcccaga cgggtataca caattcccag atgcgccacc attgtgttat
     6061 cgctggtggc gttcagttcg tcgagcgtta aatggcgttt ccagatcatg cttatgcccc
     6121 cagcgttgag ccgccatcga ccacaatatc ctgtagggtg atatggctgg cgaggtcaga
     6181 ggcgaggaac aaaatcgtgt tggcgatctc ttgtggacgg gcgattttcc ccagcggaat
     6241 gccgagttta aactgctcgc caaagccgcg aatacgctgt tcttcggcgt catcgctcac
     6301 ccacagcgtg cgttgcatat cggtgtcggt ggagccaggc gaaaccacat tacagcgcac
     6361 gccgctaacc gccagttcca gcccgacgct caacgccagg cttttcagcg ccgctttcga
     6421 tgcgccataa gcactcatac caatacgcgg cgtgtgtgcg gcgtcggacg ccacagtgac
     6481 aatcgccccg ccccgctgac ggcgaaactg gttcatggtt tgctgaaaca ggttaaacgc
     6541 accgccgacg ttaaccgcaa aagtctgctg ccagtcctct ttactgagct gatcggtcgc
     6601 gcccatgcgc aaaattcccg ccgcattgac cagcacgtcc agtcgctccg tttcagctaa
     6661 cagtcgctga catacctgcg cgacctgcgc agcatcggca acatccatca cttcggtcgc
     6721 aaagggatat tgctcctgag cgaacgcttg atcaaaaccg gtaactttcg ctcccgcttc
     6781 aacaaacgcc agcgccgtgg cgtagccgat acctttacct gcgccggtta cccagacatt
     6841 tttaccgctg aaatccatta tttcacctcg cgggagagta gcttccacca ggcgtcgatg
     6901 gtcgggtttt tcgccagcat gacaaagtcg atatcgccat gcactttgcg ccagcgcgcc
     6961 gccagcgcca tcatgcgcac cgaatccaga ccgtagtcga tcaggttgtc gtcatcgaac
     7021 ggttcatcgg actcgtccag caacggcagg ataacctcac gcagcgccgc tttcctggcg
     7081 gggacaggtg ctggcagtaa tccttcagtc atcaccaccc ggccagaccg cccggccaca
     7141 tatttcagcg acatcaaatg ctcgtcacgg ctgaaatcgg ccagcgcatc cgccaccata
     7201 aacggtttaa tatcgcgcat aaatgcgtcg gttgcggtgg tcatacagcc aatgtgggca
     7261 tataccccgg taataatcag ctggttacgt ccactctctt tgagcatttg ctccagcgga
     7321 gaacgatgaa acgcgctgta gcgccacttc accagcaccg tgtcgtcggc atctggcgtc
     7381 aggcgatcca ccaccttttg ctgttccggc gaacgggtca ggcccggccc ccacatatca
     7441 ttcaacagcg cccgatcttc atcgctctgc tctttcggct gggcggtgta ataaaccggg
     7501 atattgtgct gtttgcagta gtcgcgcagc gcagcaatat tcgcgatcac ctgctccatc
     7561 atcgggcagt tctcgcccca gaagctgaca aaatagtcct gcatatcatg gattaacaac
     7621 gcggcacgtt gcggttcaaa ggcccagtca actttattct gcggaatatc gtgagactcc
     7681 ggcagtgcgt aagcctgtaa ttttggaata gccatcgtgt tctctccttc aggctgatgc
     7741 gcgtgacgcc agccactgac gtaattgttt tttatcgact ttcccgaccg ccgtcagcgg
     7801 aagtgaatcc acacactcca cgcgatccgg taatttaaat tcggcaatac cctgttcacg
     7861 caggaaacga cgcacctgca ccgcgcgcag cggctctttt accaccagat aagcacagct
     7921 tttttcgccc atcagctcat cttccatgct caccagtgcg gcatagatca ccgccggatg
     7981 gcgcagcagc aggttttcga tctcttcggc agcgatcttc tcgccgccac ggttgatctg
     8041 atctttctcg cgcccctgca cggtgatgta accctctgga tcaatagaga tcagatcgcc
     8101 ggaacagtaa aaaccgttgg catcaaaggc actggcattg tgctgcggac ttttgtaata
     8161 accgcggaag gtgtacggcc cgcgcgtcat caggcgtcca acttcccctt gcggcagtgg
     8221 atttccttca gcatcagcaa cccatacttc atcgtccgga cacattgggt aaccctgggt
     8281 atggataatt ttctccgcgc tatcatcaag tcgggtgtag ttcaccagcc cttccgccat
     8341 gccaaacacc tgctgcaact ggcagccaat ctcagcggaa atacgcgccg caagggtggc
     8401 agaaagacgt gcgccgccga cctgtaacag tttcagcgag gcaagctgcg cccggctttc
     8461 gccttcagcc agcgcctgca accacaggct aactgccggc ggcaccagcg cggtaacgtt
     8521 aacctgatgt tttgcaatca acgggaagca aagcgtggcg ctgggatcgg ccgccagaac
     8581 aaccgttcct ccggcaagaa agacgcccag cgatcctggc gaactcatag cgtagttatg
     8641 agccgccggg atcgcgcaca ggtagcgtgt ctgttgtgtg aactgacaaa tctcgacgct
     8701 acgacgcacg ctgtagtagt agtcgttatg agtgcgcggg atcagcttcg gtgtaccggt
     8761 ggtgccgccg gaaagctgga aataggccac ttcatcagca ggtgatggcg tagcagtaaa
     8821 accgtctgcc ggatggttaa tcgcatcctg caagttatgc tcaccgctgt cgttgagcag
     8881 ttgcaccacg cgaatggagg aatgttctgt gacgaaagta ttgaggaaat catccccgct
     8941 aaacagcgca tgttggcgat cggcaatcag taacgccggt tcaatctggc tggcataggc
     9001 gttcagttca ctacgctgat ggctgaacaa cgccagcacc ggcgcaacgc ccagtttcag
     9061 cagtgcgaaa aaggtaatgt aaagttcagc gacgttaccc agttgtacca gcgcggtttc
     9121 accgggttta atgccctgac ggcgtaaact acacgcgagg ttatccgccg cctgattcag
     9181 ctcccggtaa ctcaactgtc gctcgccgtc gataaccgcg atgctgtcac tcgcagcgtg
     9241 gcgagtcaga atgtcggtca gcggcaaatc ctgccagtag cctttttccc gatagcgacg
     9301 ggcaaactct tccggccagc gggtgaatgg aatgctcatc ctcgctcctt aatgcaatcc
     9361 aaaaacgttc aacatggtag aaagtttgac gcctgtttcg cgccactcac ccaacggtga
     9421 cgacgcaggc acaatccccg ctccggcaaa cagacgcacc tgattttccc gcagcttcgc
     9481 gcagcggatg gtcaccaccc attcgccgtt accttcgctg tcacaccaac ccacaatgcc
     9541 gccaaacagt tcgcggtcaa acggctccag ttcagcaata acctgggtcg cggcctgatg
     9601 cgggaaaccg ctcagcgcgg gggtcggatg cagcagacag gccagagtca gtgcgttttc
     9661 ttgcgaattc gctttacctt caaagggagt tgcgagatgc cacagcgtcg gcgtggtaat
     9721 caattgtgga gaggagggaa cgtgtaactc actactgcgt tcgcgcagta cctctttcat
     9781 cgcctgagtc accagttcat gttcatggcg atctttttct gacgccagca gacgattacc
     9841 cgcttcgcga tcgagcactt catccggctg acgacgcgcg gaaccggcta acggaatgga
     9901 gctaaaacgc tcgccgtctt tacgtagcag cagttccggg ctggccccca gcaggacgcc
     9961 accatcagcc agcgggacat ggaagttgta actaaccggg ttttgcgcaa tcaaccgttc
    10021 cagcaatacg ccactatcaa tggcggcgtc agtggtgata tcaatcaatc gtgacaacac
    10081 cactttgtcg acctgcggcg tggcggtaag tgcggcggcg cgggcaacca tctgttcaaa
    10141 cgtggtttgt tcaggaattg cctggcgttc caccacgttc agcgactggc tgcgggtaaa
    10201 acagtgggct gaggtctgtt tttcctgacg ggagaacgac tgccaggatt cgggaatata
    10261 cagcgacgaa ggctgacgtg gatcgaaggg aatcgccccg accatcaccg gatttttgat
    10321 gccctgcgct ttggcatcgg caaacagcgc ggcgagtttt tgctggaagg gactgtcggg
    10381 cgaatccccg ttcacagccg gttcatcgaa gcgggcgaaa catcctgacg tcgtaaaact
    10441 gcggtacggc gacataaaga aaaagcgatt gggcgcaagt gttgccatgg tctgctgtac
    10501 ttcctcagcc agtgacgtat ccatatcatc ctccacaaaa tgataaaggc tttaataatg
    10561 attatcattt atattttcgg tcgctaacct aaaagcaaac gcgcactatg tcaactcttg
    10621 aggtaacgca atctgcaact cggggttgca tctggttgtg gttacaatga aaatgagaag
    10681 cattattgat gaattcgcat aagcgcaatg tgatgtcctg cgccgttttg ccccctctcc
    10741 cttccagggt gagggctggg gtgagggtta atgttcgcac cagtgctggc tgttcccctc
    10801 accctaaccc tctccccaaa ggggcgaggg gacggattgt gcgctttgtc gaatttgtca
    10861 ttacgccctt aaccttatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc
    10921 gcaacgccct tctattaaca ggacttttgc tttcaggaat agccgtagtt caggccgccg
    10981 actggccgcg tcagattact gacagccggg gcactcatac cctggaaagc cagccgcagc
    11041 gtattgtttc caccagcgtc accctgaccg gatcactgct ggcgattgat gctccggtga
    11101 tcgccagcgg cgcgaccacg ccgaataacc gcgtcgcaga tgaccagggc tttttacgcc
    11161 agtggagcaa ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg
    11221 ccgaagccgt tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggggatt
    11281 cggcgctggc gctgtatgat cagctttcca ccatcgcccc gacattaatc atcaattacg
    11341 acgacaaaag ctggcagtcg ctgttaacgc agcttggcga aattaccggg catgagaaac
    11401 aagcggcaga gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca
    11461 aattaccgcc gcagccggtc actgccattg tctataccgc cgctgcacac agtgccaatc
    11521 tctggacgcc ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga
    11581 agttgcccgc agggttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc
    11641 ttggtgggga aaatctggct gctgggttaa acggtgaatc gctattcctg ttcgccggtg
    11701 atcagaaaga cgccgatgcg atttatgcca atccactgct cgcgcacctg cctgcagtac
    11761 aaaacaagca ggtttatacg ctgggaaccg agacgttccg cctggattac tacagcgcca
    11821 tgcaagtgct ggacagactt aattcactgt taaaaaaaga ttgataaaac atacaattag
    11881 agcactaata
//
LOCUS       DAZSBF010000118        11855 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZSBF010000118 DAZSBF010000000
VERSION     DAZSBF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.119"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            3..539
                     /locus_tag="ACHXWD_002596"
     CDS             3..539
                     /locus_tag="ACHXWD_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7621482.1"
                     /translation="MAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEIHQDDIDKEVE
                     LGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFDSSAKTTGADP
                     ILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFFAWMEQIDQAT
                     PVTPGAVTANATYVLDYK"
     gene            695..2727
                     /locus_tag="ACHXWD_002597"
                     /pseudo
     CDS             695..2727
                     /locus_tag="ACHXWD_002597"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2704..3048
                     /locus_tag="ACHXWD_002598"
     CDS             2704..3048
                     /locus_tag="ACHXWD_002598"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR7621483.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3063..3794
                     /locus_tag="ACHXWD_002599"
     CDS             3063..3794
                     /locus_tag="ACHXWD_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR7621484.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3791..4852
                     /locus_tag="ACHXWD_002600"
     CDS             3791..4852
                     /locus_tag="ACHXWD_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7621485.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5004..6050
                     /locus_tag="ACHXWD_002601"
     CDS             5004..6050
                     /locus_tag="ACHXWD_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR7621486.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6047..6838)
                     /gene="nei"
                     /locus_tag="ACHXWD_002602"
     CDS             complement(6047..6838)
                     /gene="nei"
                     /locus_tag="ACHXWD_002602"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR7621487.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6874..7608)
                     /gene="pxpA"
                     /locus_tag="ACHXWD_002603"
     CDS             complement(6874..7608)
                     /gene="pxpA"
                     /locus_tag="ACHXWD_002603"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR7621488.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7598..8530)
                     /gene="pxpC"
                     /locus_tag="ACHXWD_002604"
     CDS             complement(7598..8530)
                     /gene="pxpC"
                     /locus_tag="ACHXWD_002604"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR7621489.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8524..9180)
                     /gene="pxpB"
                     /locus_tag="ACHXWD_002605"
     CDS             complement(8524..9180)
                     /gene="pxpB"
                     /locus_tag="ACHXWD_002605"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR7621490.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9203..9946)
                     /gene="ybgI"
                     /locus_tag="ACHXWD_002606"
     CDS             complement(9203..9946)
                     /gene="ybgI"
                     /locus_tag="ACHXWD_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR7621491.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10217..11698
                     /gene="dtpD"
                     /locus_tag="ACHXWD_002607"
     CDS             10217..11698
                     /gene="dtpD"
                     /locus_tag="ACHXWD_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR7621492.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2818 a         3170 c         2919 g         2948 t
ORIGIN      
        1 cactggccgt atctctgctg ttcacagcac ctgtttatgc ggctgatgaa ggatccggtg
       61 aaattcactt taaaggtgaa gttattgaag caccgtgtga aatacatcag gatgatattg
      121 ataaagaggt tgaactcggt caggtgacca ccagccacat taatcagtca catcacagcg
      181 atgccgttgc tgtcgacctg cgcttagtca actgtgatct ggaaaactcc agcaacggtt
      241 ccggtggcaa gatttctaaa gttgcagtca cctttgatag ctcagcgaaa accaccggcg
      301 cagatccgat tctcaacaac accagcacgg gtgaagccac cggcgtcggc gtacgtttaa
      361 tgaataaaga tcaaagcaat atcgttttag gcaccgctac tccagatatc gacctggctc
      421 cgacctccag cgaacaaacg ctgaatttct ttgcctggat ggaacaaatt gatcaggcta
      481 cacctgtaac gccaggcgca gttacggcga atgcaaccta cgtgctggat tataaataag
      541 tattactttg gcggcgctat cgccgccttt cataaaatat ttatcaggta tggacaccgt
      601 gaatatttat cgactctctt ttgtatcctg cctggtcgtg gcgatgcctt gcgcattggc
      661 ggtcgaattc aaccttaatg ttctcgataa atcgatgcgc gaccgcattg atatttcatt
      721 attaaaggaa aaaggagtca ttgctcccgg tgagtatttt gttagcgttg cggtaaataa
      781 taaccaaatc agtaacgggc aaaagattaa ctggcacaaa aatgacgata aaaccattcc
      841 gtgcatcaat gatttactgg tcgataaatt tggcttaaaa cctgaagtcc gtcagtcgtt
      901 accattgata aatcagtgcg tcgattttag ctcccgacct gaaatgctct tcaatttcga
      961 tcaagccaat cagcaactga atattagtat tccgcaagcc tggctggcgt ggcactcaga
     1021 aaactggacc ccaccctcca catggaaaga aggtgtcgcc ggtatcctga tggattacaa
     1081 cttgtttgcc agcagctacc gcccacagga cggcagcagc agcactaacc tgaacgccta
     1141 cggtaccacc ggaattaacg ccggggcatg gcgcttacgt agtgattatc agttgaatca
     1201 gactgatagc gatgataacc atgaacagtc aggcggaata tcgcgcacct atctttttcg
     1261 tccattaccg caattaggct ctaaattaac cctcggcgaa acggatttta gttccaatat
     1321 tttcgacggt ttttcttata ccggcgcggc actggcaagt gacgagcgaa tgttgccatg
     1381 ggaactacgc ggctacgccc cacaaattag cggtattgca cagaccaatg ccacggtgac
     1441 gatcagtcaa tcaggccgcg tcatttacca gaaaaaagtc ccaccaggcc catttatcat
     1501 tgacgacctt aatcagtctg ttcagggcac actggatgtc aaagtgacgg aagaagatgg
     1561 tcgggtgaac aatttccagg tttcggcagc atcgacgccc ttcctgactc gtcagggaca
     1621 ggttcgctat aaactggccg cgggtcagcc acggccctcc atgtcacatc aaactgaaaa
     1681 tgaaaccttt tttagcaatg aagtttcctg ggggatgctg tcaaacacct cgctgtacgg
     1741 cggcctgctg ctttctggtg atgactacca ttctgccgca atgggtattg ggcaaaatat
     1801 gctgtggctt ggtgcgctgt cgtttgatgt cacgtgggcc agtagccatt ttgatactca
     1861 gcaggacgag cggggcttaa gctaccgttt taattacagc aaacaagtgg atgccactaa
     1921 cagcacgatt tccctcgccg cttatcgttt ctccgatcgt cattttcaca gctacgccaa
     1981 ctatctggat cacaaataca acgacagcga tgcgcaggac gaaaaacaga cgatcagctt
     2041 attgtgggcc aaccgattac cccactaaac ctcaatcttt acgccaacct gctacatcaa
     2101 acctggtgga atgcagacgc ctccacgacc gccaacatca cggcaggttt taatgttgat
     2161 attggtgact ggagagatat ctctatttcg acgtcattca atacaaccca ttacgaagat
     2221 aaagatcacg acaaccagat ttacctgtcg atttcgctcc ccttcggtaa cggtggtcgg
     2281 gttggctatg acatgcaaaa cagtagccac agcaccacac accgcatgtc gtggaacgat
     2341 acgctggatg aacgtaatag ctggggcatg tctgccggac tgcaatccga ccgtcctgac
     2401 aatggagccc aggtgagcgg taactatcag cacctgagtt cagcgggtga gtgggatatt
     2461 tctggtacct atgccgccaa tgattacagt tccgtcagca gcagctggag cggttctttc
     2521 accgcaaccc aatatggtgc agcgtttcat cgccgcagct ccaccaatga accacgcctg
     2581 atggtcagca ccgatggcgt ggcagatatt ccggttcagg gcaatctcga ctacaccaac
     2641 cattttggca ttgcggtggt gccgttgatt tccagttacc agccttccac cgtggcggtg
     2701 aacatgaatg acttacccga cggcgtaaca gttgcagaaa acgttatcaa agaaacgtgg
     2761 attgaaggcg cgataggtta caaatcactg gcttcccgtt ccggtaaaga cgttaacgtc
     2821 atcattcgca acgccagcgg tcagtttcct cccctcggag cggatatccg ccaggatgac
     2881 agcggcatta gcgtggggat ggttggcgag gaaggacatg cctggttaag cggagtcgct
     2941 gaaaatcaaa agtttaccgt ggtctggggt gatagccagc attgctcgct ccatcttcct
     3001 gaacatatgg aagacaccgc aaatcgcctg attttacctt gtcattaatt aaggaaatga
     3061 aaatgacatt tatgaaagga ctaccccttt tgttgttggt tgccagtctg tgcagccacg
     3121 ctgcactaca acccgatcgc actcgtattg tgtttaacgc taatgataaa gctaccagtc
     3181 tgcgggtgga taatcgtagc gataaactcc cctacctcgc ttattcctgg ctcgaaaatg
     3241 aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc gccgatacaa cgtctggaac
     3301 cgaaagcaac aacgcaggta cggatagtaa agcaagcctc gaccacaaaa ttaccgggcg
     3361 atcgcgaaac gctatttttc tacaatatgc gtgaaattcc tcccgccccg gaaaaaaaca
     3421 gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat caaggtgttc tggcgaccgg
     3481 ctgcattacg caagaaagca ggagaaaagg tcgaactgca gttacaggtc agccagcagg
     3541 gcaaccaact gacactgaaa aatcctaccg cgtattatct gaccattgcc tacctgggac
     3601 gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat ggttgcgcct tttagtacgg
     3661 tcaacacaaa caccggaaat tatagcggca gccagtttta tctcggttat atggatgatt
     3721 acggcgcgtt acgcatgacg acgctcaact gcagcggaca atgccattta caagccgtgg
     3781 aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc atctgtctgt tatttctgcc
     3841 gttgaagtct gcgcttgccc tgaactgcta ctttggttca tccggtggct cggtagaaaa
     3901 atcagaagcg attcaacctt ttgctgtacc aggcaatgcc aaactcggcg ataagatttg
     3961 ggaatctgac gatattaaaa ttcccgtcta ttgtgacaac aataccaacg gcaattttga
     4021 aagtgagcat gtttatgcct gggtaaaccc ctacccgggg gtgcaggatc gctattatca
     4081 actcggcgta acttacaatg gcgttgatta tgacgccaac cagggaaaaa gccgcatcga
     4141 cactaaccag tgtattgaca gtaagaacat tgacatttat acccctgagc agattatcgc
     4201 gatgggatgg cagaacaaaa tttgctcggg tgatcccgct aatattcaca tgtcgcgtac
     4261 ctttctcgct cgcatgcggt tatacgtcaa aatacgagaa atgccgccgc atgattatca
     4321 aagtacgctt agcgactata tcgtcgtgca atttgacggt gccggtagcg ttaacgaaga
     4381 ccctactgcc caaaacctga aatatcatat tactggtctg gaaaacattc gcgtgctgga
     4441 ttgcagcgtc aatttttcca tttcaccgga aacacaggtg attgattttg gtaaatttaa
     4501 tttgctggat attcgtcgcc acacaatgtc gaaaacattc agtattaaga cgacaaaatc
     4561 acaaaatgac caatgcaccg acggatttaa agtcagttcc tccttttata ccgaagaaac
     4621 gttggttgaa gaagataaag cattgttgat tggtaatggt ctgaagctac gtttgctgga
     4681 tgaaaacgcc tcgccttata ccttcaataa atatgctgaa tacgccgatt tcaccagcga
     4741 catgttggtc tacgaaaaaa cctatacagc tgaactttcg tccattgcag gtacccccat
     4801 cgaggctggg cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat
     4861 gatgcccttt gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc
     4921 cttgattgct gttatttttt cctggcccgg ctttctttat gctgtatcga tacaccaaag
     4981 agaaaataat gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat
     5041 cacttctcct ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc
     5101 cgatgatcgc cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg
     5161 cttttctcgc cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga
     5221 ttctcaccac gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc
     5281 tttccagcgc catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg
     5341 gtgcctgggg ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg
     5401 gcgcagatat tcgcctggtt gcatttatgc aatatctgcg agtgctgttc gtcgcggggg
     5461 ccgctgttct ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa
     5521 tcgtctggtt cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg
     5581 ccggtacggc gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg
     5641 cgggcgcagt gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg
     5701 cgatggcgta tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac
     5761 tgcgggcact acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt
     5821 gtgcgggtat ggcgtggggg ctgacccggt ttatgcacat tgattttatg actgcctacc
     5881 tcgccaccag tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg
     5941 atatggcgct tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacggggc
     6001 cagccattgc gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc
     6061 cagggcacca gtaaaacggg cgagatgaca gcgtggtttt ctcaatgatg ctgccacagc
     6121 gttcgcacgg ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct
     6181 tattctcatc caccagcccc ctcgtagcgt aggaaaatcg aggaatatcc agtaacgcgt
     6241 gtgcgagtgc atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc
     6301 caacctgcca gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat
     6361 cgagcagtaa tcctgcaaac tgacggttac gaaagcgcgg tgacaataac cgttccttta
     6421 ccacctccgg cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat
     6481 gcgtggtcag ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg
     6541 ttttgtcagc cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg
     6601 tatcaaccac gcgccagacg ccgtaaagct gattatggct gtagagcgtt aagtcgttgg
     6661 aaaaatgagt taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa
     6721 gttgtgattg ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc
     6781 ctttgatcgc cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg
     6841 tgatatcctt ttttcattct ttattaatcg gggttatgct gcgacaacaa tctccttttc
     6901 ggcaaatgta gagcgtagtc ggcgggcgaa cgccagtgcg tgctcgccgt cgccatgcag
     6961 gcagactgtt tgcgccgtga ccgttgccca ttcgccggtg atacttttga ctctgccgtg
     7021 ttgcaccatc tctagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctcg
     7081 ctgacttcgc ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc
     7141 gcgcgttgtc aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac
     7201 gagaatcaat gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc
     7261 ttctttcgcc gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc
     7321 ttgcgcacgg gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt
     7381 ttctggcggc agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg
     7441 agcgccaatc gcgacaccat tttttattgc ttcacgcacg caagcctgca tgatttgcgc
     7501 atcgcctgca tgaaatccac aggcaatatt ggcagaggaa accagcgtta atagctctgc
     7561 gtcgctggcg cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc
     7621 acactaattg ttcgaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg
     7681 aacactggac aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag
     7741 cctcaatgat acaggcaata cgcgggtaac catcggtggt ctgtgcgtcg ttcatcaaca
     7801 caatcggctg cccgttatgt ggcacctgca ccacgcccgg taacaaaccg tgagataaca
     7861 gttcgcgatc ggtggtgcgt tttaaaattt gcccctgtaa gcgatagccc atgcggttac
     7921 tttgcgagct aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact
     7981 catgatattc cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc
     8041 cctgcgcttc cataaaatca cgcttcgatt tgccaatcgg gagtcggtca ccatccttca
     8101 gtaaacggcc ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta
     8161 ccggcggaac atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc
     8221 gtttaagcgt taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcat
     8281 tatcatccag ccgcgcttcg caaccggcac ccgtcagagc aaaccaccca tcagtttcga
     8341 actcaaccgt taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca
     8401 ccagcaggtt agcaatgcgt aacgcaggca tatccagtgc gccgcagtgg ctgattcccg
     8461 actggcgaaa accgtgacga ccgccatcct gcacagtggt atacatgccc gcacgaataa
     8521 tcttcagcat actccctcct tctgcggtac aaagcgcacg ctgtctcccg gacgtaataa
     8581 gatgggttcg tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca
     8641 gccacccggc gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc
     8701 tgccggaacg agcaagcgcg gttcagcgcg ccgtggcgtg tgtagttgtt ccggcaaact
     8761 cccgagatac gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga
     8821 gtgcaattca acaacctgtt tttcgctaaa cccgcaatgc gccgcgacca ccgccaaatc
     8881 cggtccgcct gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag
     8941 ggcctcgctc tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc
     9001 aggattacgc aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg
     9061 catatccacc agacgctgcg ccagtcgcca gatccgtttc tggctagcca gcgtcaccgg
     9121 cggttccagt tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac
     9181 tttcgatccc tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa
     9241 gatcggtatt ttcattcagc cactcgctca atgcgcgaat accaccacgt tcagtggcat
     9301 ggtgacctgc agcataaaaa tgcaacccct gctcgcgggc tgaatgaatg gtctgttcag
     9361 aaacttcgcc agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt
     9421 gcccgccgcc cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc
     9481 ataacggctt acgtcccaga cgcgcttcaa tccaggaggc tagttccagt cccggcacag
     9541 gcatggtcag ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca
     9601 gtaacgccgc cagctgtgcg ttattgccca gctcaggatg tacgtcaagc ggcaaatgcc
     9661 agccatacag gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc
     9721 gaatgaccgg agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc
     9781 ccagacgcac tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt
     9841 gcaccgtctc tttgccttct acctgcaaac cattcggcgc gtaatcacta atcgccgcgc
     9901 tgttcagttt ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg
     9961 tattctgata gctacaccgt taacatagcg ttacctcgcc tgttcgtcga tagccaatct
    10021 tttgacatat taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc
    10081 ttcagctaat gtatatttat gcaatcagac agcatattgc ataaataatc agatctgtat
    10141 caaagtttta actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgcagt
    10201 caattgtgga attattatga ataaacacgc atcacaaccg cgcactattt actatgtcgt
    10261 tgcgctgcaa atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta
    10321 tctcaccaat caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg
    10381 ttcgctggtg tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa
    10441 tcgcatggcg gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc
    10501 cagtgagatc catccgtcat tcctctatct gtccctggcg attatcgttt gtggctatgg
    10561 tctgtttaaa tccaatgtca gttgcctgct cggcgagctg tatgagccaa ccgatccgcg
    10621 tcgtgatggc ggtttctcgc tgatgtatgc agcgggtaac gtggggtcta ttatcgcacc
    10681 tatcgcctgt ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc
    10741 ggtgggtatg atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcacacatac
    10801 ccgcggcgtt aataaaaaag tactgcgtgc aacaaacttt ctcctgccta actggggatg
    10861 gctgctggtt ctgctggtgg caacgcctgc gctgattacc gtactgttct ggaaagagtg
    10921 gtcggtatac gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta
    10981 tcgcaaagca gaaaaccaga aacagcgtaa ggagctgggg ctgattgtga cgctcacctt
    11041 cttcagtatg ttgttctggg ccttcgcaca acagggcggc agctcaatta gcctttatat
    11101 cgaccgcttc gttaaccgcg atatgtttgg ttataccgtt ccgactgcta tgttccagtc
    11161 gattaatgcc ttcgcagtta tgctatgcgg tgtgttcctg gcgtgggtgg ttaaagaaag
    11221 cgtcgcaggt aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat
    11281 gagcgccgga ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc
    11341 ttctctgcca ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga
    11401 cccggttgcc atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac
    11461 cggcatctac atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga
    11521 tcagacatcg caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata
    11581 tattgaagta tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat
    11641 gatttggctg tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata
    11701 ggccagcacc gatgcctgat gcgacgcttg acgcgtctta tcaggccggt gatgctgcca
    11761 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
    11821 agctgtccct cctgttcagc tactgacggg ggggt
//
LOCUS       DAZSBF010000119        11816 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZSBF010000119 DAZSBF010000000
VERSION     DAZSBF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.120"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHXWD_002608"
     CDS             <1..389
                     /locus_tag="ACHXWD_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR7621493.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
     gene            complement(588..938)
                     /locus_tag="ACHXWD_002609"
                     /pseudo
     CDS             complement(588..938)
                     /locus_tag="ACHXWD_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHXWD_002610"
     CDS             complement(1141..2154)
                     /gene="intI1"
                     /locus_tag="ACHXWD_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR7621494.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHXWD_002611"
     CDS             2312..2785
                     /gene="dfrA17"
                     /locus_tag="ACHXWD_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR7621495.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2916..3704
                     /locus_tag="ACHXWD_002612"
     CDS             2916..3704
                     /locus_tag="ACHXWD_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR7621496.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3910..4257
                     /locus_tag="ACHXWD_002613"
     CDS             3910..4257
                     /locus_tag="ACHXWD_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR7621497.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4251..5090
                     /gene="sul1"
                     /locus_tag="ACHXWD_002614"
     CDS             4251..5090
                     /gene="sul1"
                     /locus_tag="ACHXWD_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR7621498.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5218..5421
                     /locus_tag="ACHXWD_002615"
     CDS             5218..5421
                     /locus_tag="ACHXWD_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621499.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5577..6782
                     /gene="chrA"
                     /locus_tag="ACHXWD_002616"
     CDS             5577..6782
                     /gene="chrA"
                     /locus_tag="ACHXWD_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR7621500.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6793..7098
                     /locus_tag="ACHXWD_002617"
     CDS             6793..7098
                     /locus_tag="ACHXWD_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR7621501.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            7325..8089
                     /locus_tag="ACHXWD_002618"
     CDS             7325..8089
                     /locus_tag="ACHXWD_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR7621502.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHXWD_002619"
     CDS             complement(8582..9166)
                     /gene="mphR(A)"
                     /locus_tag="ACHXWD_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR7621503.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHXWD_002620"
                     /gene_synonym="mrx"
     CDS             complement(9166..10404)
                     /gene="mrx(A)"
                     /locus_tag="ACHXWD_002620"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR7621504.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10401..11306)
                     /locus_tag="ACHXWD_002621"
     CDS             complement(10401..11306)
                     /locus_tag="ACHXWD_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR7621505.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            complement(11428..>11816)
                     /locus_tag="ACHXWD_002622"
     CDS             complement(11428..>11816)
                     /locus_tag="ACHXWD_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR7621506.1"
                     /translation="SSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALL
                     KREGRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRAL
                     RKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT         2490 a         3391 c         3450 g         2485 t
ORIGIN      
        1 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
       61 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
      121 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
      181 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
      241 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
      301 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
      361 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
      421 cgctaacttt gcaacagtgc ccccaatgcg ctgtccagtc atggaattcc ctgcaaaatg
      481 tcagggaaga ctctatgacc ttcaacgaga tatgtcaata aattcaaaat tcaatcctat
      541 cctgacgcaa tttacacatg gcatctgaca tcaggttagg gtatgcctca acctgacggc
      601 ggcgaatcac aagcgtccgg tttgacgctg gtttcggtcg cagtgctggc ccgcgcctgg
      661 aagcgctgat agcactccag cccgcagaaa tgctccacgt actcggcccc ttccggcgtg
      721 aaggcggcat cgagcgggat ttccttgcag cacacgcagc aactggtgct cggttcattg
      781 gcgttcatgg tggtgttcct ccattggttg acgaagccga cgaaggccgc cgccggcatc
      841 ggcctggcga acaggaaacc ctgcaccgtg tcgcaacccg cctgccgcaa ccacgcaagg
      901 cagtcgggtg tttccacacc ctcggctacc acctccattg tcgttttcag aagacggctg
      961 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatcc atcaggcaac
     1021 gacgggctgc tgccggccat cagcggacgc agggaggact ttccgcaacc ggccgttcga
     1081 tgcggcaccg atggccttcg cgcaggggta gtgaatccgc caggattgac ttgcgctgcc
     1141 ctacctctca ctagtgaggg gcggcagcgc atcaagcggt gagcgcactc cggcaccgcc
     1201 aactttcagc acatgcgtgt aaatcatcgt cgtagagacg tcggaatggc cgagcagatc
     1261 ctgcacggtt cgaatgtcgt aaccgctgcg gagcaaggcc gtcgcgaacg agtggcggag
     1321 ggtgtgcggt gtggcgggct tcgtgatgcc tgcttgttct acggcacgtt tgaaggcgcg
     1381 ctgaaaggtc tggtcataca tgtgatggcg acgcacgaca ccgctccgtg gatcggtcga
     1441 atgcgtgtgc tgcgcaaaaa cccagaacca cggccaggaa tgcccggcgc gcggatactt
     1501 ccgctcaagg gcgtcgggaa gcgcaacgcc gctgcggccc tcggcctggt ccttcagcca
     1561 ccatgcccgt gcacgcgaca gctgctcgcg caggctgggt gccaagctct cgggtaacat
     1621 caaggcccga tccttggagc ccttgccctc ccgcacgatg atcgtgccgt gatcgaaatc
     1681 cagatccttg acccgcagtt gcaaaccctc actgatccgc atgcccgttc catacagaag
     1741 ctgggcgaac aaacgatgct cgccttccag aaaaccgagg atgcgaacca cttcatccgg
     1801 ggtcagcacc accggcaagc gccgcgacgg ccgaggtctt ccgatctcct gaagccaggg
     1861 cagatccgtg cacagcacct tgccgtagaa gaacagcaag gccgccaatg cctgacgatg
     1921 cgtggagacc gaaaccttgc gctcgttcgc cagccaggac agaaatgcct cgacttcgct
     1981 gctgcccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2041 gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct cacgcaactg
     2101 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2161 ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     2221 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     2281 taaaacaaag ttagccatta agggagttaa attgaaaata tcattgattt ctgcagtgtc
     2341 agaaaatggc gtaatcggta gtggtcctga tatcccgtgg tcagtaaaag gtgagcaact
     2401 actctttaaa gcgctcacat ataatcaatg gctccttgtc ggaagaaaaa catttgactc
     2461 tatgggtgtt cttccaaatc gcaaatatgc agtagtgtca aagaacggaa tttcaagctc
     2521 aaatgaaaac gtcctagttt ttccttcaat agaaaatgct ttgaaagagc tatcaaaagt
     2581 tacagatcat gtatatgtct ctggcggggg tcaaatctat aatagcctta ttgaaaaagc
     2641 agatataatt catttgtcta ctgttcacgt tgaagtcgaa ggtgatatca aattccctat
     2701 aatgcctgag aatttcaatt tggtttttga acagtttttt atgtctaata taaattatac
     2761 ataccagatt tggaaaaaag gctaacaatg cgttgcagca ccagtcgctt cgctccttgg
     2821 acagctttta agtcgcgtct ttgtggtttt gctgcgcaaa agtattccac aaagccgcaa
     2881 cttaaaagct gccgctgaac ttaacgttag gcatcatggg tgaatttttc cctgcacaag
     2941 ttttcaagca gctgtcccac gctcgcgcgg tgatcgagcg ccatctggct gcgacactgg
     3001 acacaatcca cctgttcgga tctgcgatcg atggagggct gaagccggac agcgacatag
     3061 acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggcg ctaatgctcg
     3121 atttgctgaa agtctcatca ccgccaggcg atggcggaac atggcgaccg ctggagctaa
     3181 ctgttgtcgc tcgaagcgaa gtagtgcctt ggcgctatcc ggcgcggcgt gagcttcagt
     3241 tcggtgagtg gctccgccac gacatccttt ccggaacgtt cgagcctgcc gttctggatc
     3301 acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgcgctt ctaggcccat
     3361 ccgcagccac gtttttcgag ccggtgccga aggagcattt ctccaaggcg cttttcgaca
     3421 ctattgccca gtggaatgca gagtcggatt ggaagggtga cgagcggaac gtcgttcttg
     3481 ctcttgctcg catttggtac agcgcttcaa ctggtctcat tgctcctaag gacgttgctg
     3541 ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc cctcatctgc aaggcacgcg
     3601 cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag acggccgcgt
     3661 tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcggca tgtgcgaaaa
     3721 gtgcatcgac ccgcgccgag ggcatctgat gcctaactcg gcgttcaagc ggacgggctg
     3781 cgcccgccgc tcaactatgc gttagatgca ctaagcacat aattgctcac agccaaacta
     3841 tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg
     3901 agatatatca tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca
     3961 acatccgcat taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata
     4021 atcggttatg gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt
     4081 gttgcttatg cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg
     4141 cttcatgggc aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc
     4201 tttttgctcg cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg
     4261 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
     4321 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
     4381 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
     4441 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
     4501 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
     4561 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
     4621 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     4681 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     4741 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     4801 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     4861 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     4921 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     4981 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     5041 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt
     5101 ccggccgccc gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg
     5161 ctcgtcagga tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg
     5221 gacagcgagg agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg
     5281 cggctgatgc acgacgctgc ggcgtggatg tccgccaagg gaacgcccgc ctgggacgtc
     5341 gcgcggatcg accggacatt cgcggagacc ttcgtcctga gatccgagct cctagggatc
     5401 gcctcagaaa acggaaaata aagcacgcta agccgtaagt aagcgtgctc ctgtgaaagc
     5461 cacagctaaa actgcgtagt acacatagag gtattttttt ctagatttat atcgaatttc
     5521 gttatcgaat ttcgcttatt attgatcaac tcgttgcaga tcatgaaggc gaaagtatga
     5581 cgaataaccc taccgatgac agcagaccat ggtcggtctt tcttattttt ctgcggcttg
     5641 gattgacatc ttttggcggc cccattgcgc acttgggcta cttccgcgcc gaatttgtca
     5701 cacggcggcg ctggctctcc gaacggagct atgctgactt ggtcgcgctt tgtcagttct
     5761 tgccagggcc tgcaagcagc caggtcggca tagcggtagg actgtctcgg gctggataca
     5821 gcggggcgct ggctgcttgg gctggcttca cgctgccgtc tgccatagcc ttgatccttt
     5881 ttgcgctcgg catctccagc tatggcgatt acgtctcgca gggcgcgttg catggcttaa
     5941 aagtggtggc tgtggccgtg gtcgctcaag cagtatgggg catggcgcgt aacctatgca
     6001 cggatgggct gcgagtcacc atcatggcaa ttgctacctg cgtcgtttta cttgtgccgt
     6061 ccgcgtgggg acaggttggc gtgattgcta tcgcaggcat cgcaggccgg ttattgttca
     6121 agccagcgaa agttgttgag catgaccccc tacctatcac ggtcagtcac cgggccggcg
     6181 tgctttggct ctcgctgttc tttgtcttgc tgattggcct gccggtgttg gccgaactga
     6241 tgccaagtca aaccatggca atggtggatt ccttctatcg tgtcggatca ctggtgttcg
     6301 gcggtggtca cgttgtgctg ccattactgc aagccgaagt ggtgccctcc ggctgggtca
     6361 acaatgaatc ctttctcgcg gggtacgggg cagctcaagc ggtgcccggc cctttgttca
     6421 cgttcgccgc gtttcttggt gcctcgatga acaccgcccc gtcgggctgg atcggcggca
     6481 ttgtgtgtct gctggctatc ttcgcgccct cgttcttgct ggtcgtcgga tcaatgccat
     6541 tttgggagcg tttgcgccgc aatacaggca tccaagctgc gctggccggg atcaatgccg
     6601 ctgtagtcgg cttgctgctg gccgcgctgt atcagcctgt atggactagc gccatctttc
     6661 agccgcaaga cttcggcttg gcattagttg cccttgtcgc acttatgttc tggaagctcc
     6721 cgccgtggct ggttgtcctc ggtagcggtg cagctgggtg gctgttgagc gtcgctctgt
     6781 gaggcacaaa aaatgactga caaagacctc tacggcggtt tgatccgcct gcacatcctt
     6841 caccatgcag ccgaggaacc tgtctttggg ctggggatca tcgaagagct acgccgacac
     6901 ggctacgaga tgagcgctgg caccgtgtac ccgatgctgc acggcctgga aaaaaaaggc
     6961 tatctgacct cacgccacga acgcaccggg cgacgtgaac ggcgtgttta tgacatcact
     7021 gaacaaggca gaactgcact tgctgatgcg aaaacaaagg tcaaggagct gttcggagag
     7081 ttggtagaag gtggttgagt ttttgcttct atgtcaggtt gagctatacc ctaactggat
     7141 tgtcattttc agaagacgac tgcaccagtt gattgggcgt aatggctgtt gtgcagccag
     7201 ctcctgacag ttcaatatca gaagtgatct gcaccaatct cgactatgct caatactcgt
     7261 gtgggctctg ttgcaaaaat cgtgaagctt gagcatgctt ggcggagatt ggacggacgg
     7321 aacgatgacg gatttcaagt ggcgccattt ccagggtgat gtgatcctgt gggcggtgcg
     7381 ctggtattgt cgctatccga tcagctatcg cgaccttgag gaaatgctgg cggaacgcgg
     7441 catttcggtc gaccatacga cgatctatcg ctgggtccag tgctacgccc cggagatgga
     7501 gaagcggctg cgctggttct ggcggcgtgg ctttgatccg agctggcgcc tggatgaaac
     7561 ctacgtcaag gtgcggggca agtggaccta cctgtaccgg gcagtcgaca agcggggcga
     7621 cacgatcgat ttctacctgt cgccgacccg cagcgccaag gcagcgaagc ggttcctggg
     7681 caaggccctg cgaggcctga agcactggga aaagcctgcc acgctcaata ccgacaaagc
     7741 gccgagctat ggtgcagcga tcaccgaatt gaagcgcgaa ggaaagctgg accgggagac
     7801 ggcccaccgg caggtgaagt atctcaataa cgtgatcgag gccgatcacg gaaagctcaa
     7861 gatactgatc aagccggtgc gcggtttcaa atcgatcccc acggcctatg ccacgatcaa
     7921 gggattcgaa gtcatgcgag ccctgcgcaa aggacaggct cgcccctggt gcctgcagcc
     7981 cggcatcagg ggcgaggtgc gccttgtgga gagagctttt ggcattgggc cctcggcgct
     8041 gacggaggcc atgggcatgc tcaaccacca tttcgcagca gccgcctgat cggcgcagag
     8101 cgacagccta cctctgactg ccgccaatct ttgcaacaga gcccgccgtg ctagtctgct
     8161 cggtgatggt ggagtgaagc caacccgcaa tcgggttatg aatctgcatc gcgattcgca
     8221 atcagctgtc tcttgagcat gtcgaactcc tgcgatgtta tctcgccgtg ctcgtgctgc
     8281 tacaccaact tgtccaactc atccgccaca ccgctcccca ccacctgcga tggccaggtt
     8341 gtttgctggg cgcattggcg gagtatcgtc tcgacccggg acacccacac cggcacaacc
     8401 ttacgggagt gattcactgt caaagaatcg gcccggtgct ctgacgcaag tatcgggatg
     8461 gtcaccattt gtaagccgta gacctgagtg gtgatcaaga cttcgatacc accgaccgta
     8521 ccggtactaa tcgacgacgg tcgtgttcgt cgcctgccgc agggactctg cacacctccg
     8581 tttacgcatg tgcctggagg agttggaaat cgtcgtgttc gggaaacatt aaacacagga
     8641 tggcagcgat ctgagccagc acatgatcag ctagctcacc atccggatcg acggcccact
     8701 gcatcgtcgc gccagcgatg accgagtgca ggagcaactc agctgccgca ggagcacctg
     8761 ggggcagtcg cttgcggatc ccctccacca ccgcgcggtt ccgctggatc gcaagcgtgc
     8821 gtagctccgg cacctggagc tcgtaccagg agatgagata gttcaccgag aagtcgttgc
     8881 gagtgttcat gctccgaacg agcacctgca aaaattccca gagcccttgc ggccctgcgc
     8941 ctatcggtat cgcattcagg taatgccgca cctgctcgac gccgcgctcc atcatcctca
     9001 ccagcagcgt atcgcggttg gtgaagcgct ggattaacgc tgcgcgggag agccccacct
     9061 cctttgctac tccgctgagc gtgaactcta tgggaccgca acgcttcagc actacggtgg
     9121 cggcctcgag tacctcgtca tcggacttga gcttggggcg gggcatcagt gttcaccttc
     9181 tgtatgggtt ggggcggagg ctgtggctgc cgccgccatt gtagcaaatt gaagacggag
     9241 cgagagtaga gccacgagcc ccgcaaacac ggccgataca acgaggccag ggagcggacc
     9301 agcaaggtcg acaaacgcgc cggccgcaag cataaccatg ggcgaggctg acagcatcac
     9361 cgccgagacc gtgccgagta cccggccgag aagttctggc ggcgtgcggt tgtagatggc
     9421 agcgttgaga atgggagaga ctgagccggt cagcagtccc acgagcgcgc ccaacaacat
     9481 cagcaccggc acgcctggca actgtgaaag cagaagcgag cccaccgcag agccacaaaa
     9541 tgccaccgcc agccagttct gcgctgatat ccgggcgccg accgacgcat gaatggcaat
     9601 gccaaggaga ccaccagccc ccatcattga ggagaacagc ccgagctctg ctacttggcg
     9661 tcctgcatct acaaacagcg caggcatgat gacgctgccg ttggcgccaa cgatgcccac
     9721 gaagatcatc actataccaa agagagggcg cagcaggggt tcgctccaga gaaaagcgac
     9781 gccggcgcgc atggagagag tcgccgtcgt ggtcatcgtc cgagcggcac gcgcgggaag
     9841 cacccacgcg ccgagcagac ctgcaaggac ggagcagaac gccgtcagcc cgagcgttgg
     9901 cgcagcgcca agcaggccga ttgcggcccc cccaagggcc gggccaccta gaatcgcgac
     9961 gttcccgatc accgctttca gtgacgagac gcgctcaacg gagagcccgg cgacgtggcc
    10021 gagtttgggc agctcactgt cctgcgcggc cataccgggt gcgtcgaacg cggcaccgag
    10081 caccacgcaa gcgatcagcc cagtgttcga gagggcgcca acggcatcga gcagtgggat
    10141 gctcgccatg gccacgccgc ccaccacacc cgagatcaat gcgacgggcg cgcgcccgaa
    10201 ccgatcgacg aggccaccac caacccacgc gccgatgatg gtcgcgatga cgctgctagc
    10261 ggccgtggcg cccgcccagg ccgcgctctt tgtatgagac aggacgaacc atggaagcgc
    10321 gagggccgcc accgcgttgc cgatccggaa gagaaaggtc gccgcgaaca gcgtcgcgag
    10381 cgggctatat cgacgttcgc tcattccgct gcggcgagct gcgccttcgc cgcagcgagg
    10441 tactcttcgt tacccgagtc gagggcgaag agtgcgtagg tgaccgcccc gaacgcaagg
    10501 cgctccgcga tgtggtgggc gagccgcggc cacacccggc caccggccgc ttcatacgtg
    10561 aggaggagct tcgcgagccc ctcttcacca aagaccataa ggtgcgcggc catgtcgatg
    10621 gcagggtcat caacgcgggc ctcgctccag tcgatcatcc cgctgacgcg ctccgtgttg
    10681 tcgatgagca catggcccac gtagagatcg ccatgcacca ccacggagaa atctggccac
    10741 gacgaatcgt cgtcgagcca gcgctgccac cggtggaggc gcttgtcgtt caccacgaac
    10801 tcgcgtcgga cgcggtcaac gtcgtcggcc accttctgac gggcctgcgt cggtgtacgg
    10861 atgagcatcc ccgcatccac ggcggcggaa atggggacgg catgcagggc ggcgagcgcg
    10921 gtcgcgaagc tctccgcgaa gacctccgag tcctgcggca cgacccagtc gggcgtggac
    10981 gaaccaggct ggatgaccat cgcagtcgag tcttcgagca tgggataggc aacgagctcg
    11041 gcgttggcca cgcgccagtc cggcaccgcg aacggcaggc gattcttgag cattgccagc
    11101 acccgcgcct ctggttcgac cttcgcgctt acctcggctc ggcgcgggat gcgcagcacc
    11161 caccgacgtc catcgtcgac ggtggcgatc acgatcctat agtcgagccc aagctcattg
    11221 acagtcagcg ggccatggag cttgagccca tgtcgggctg caagtgcgta cagttgggag
    11281 gtatcggcgg tcgtgactac ggtcatgatt cactcctgag ggcttgacgg gtttagccac
    11341 ctaaatgtaa cagtcacgtc ggttatattc aatccggcac tgttgcaaag ttagcgatga
    11401 ggcagccttt tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat
    11461 ttcgcccagg ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat
    11521 cacctcaata cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc
    11581 gccgattatc cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat
    11641 ctgtcggtgt tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg
    11701 accataggcg ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt
    11761 gttgaggatt ttacccagaa accggtatgc agctttgctg ttacgacggg aggaga
//
LOCUS       DAZSBF010000120        11777 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZSBF010000120 DAZSBF010000000
VERSION     DAZSBF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.121"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..609)
                     /locus_tag="ACHXWD_002623"
     CDS             complement(<1..609)
                     /locus_tag="ACHXWD_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR7621507.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(732..2378)
                     /gene="fumB"
                     /locus_tag="ACHXWD_002624"
     CDS             complement(732..2378)
                     /gene="fumB"
                     /locus_tag="ACHXWD_002624"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR7621508.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="ACHXWD_002625"
     CDS             complement(2456..3796)
                     /gene="dcuB"
                     /locus_tag="ACHXWD_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR7621509.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="ACHXWD_002626"
     CDS             complement(4367..5086)
                     /gene="dcuR"
                     /locus_tag="ACHXWD_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR7621510.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5083..6714)
                     /locus_tag="ACHXWD_002627"
     CDS             complement(5083..6714)
                     /locus_tag="ACHXWD_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR7621511.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6895..7125
                     /gene="yjdI"
                     /locus_tag="ACHXWD_002628"
     CDS             6895..7125
                     /gene="yjdI"
                     /locus_tag="ACHXWD_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR7621512.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7137..7409
                     /locus_tag="ACHXWD_002629"
     CDS             7137..7409
                     /locus_tag="ACHXWD_002629"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7621513.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7636..7932
                     /gene="ghoS"
                     /locus_tag="ACHXWD_002630"
     CDS             7636..7932
                     /gene="ghoS"
                     /locus_tag="ACHXWD_002630"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR7621514.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7960..8133
                     /gene="ghoT"
                     /locus_tag="ACHXWD_002631"
     CDS             7960..8133
                     /gene="ghoT"
                     /locus_tag="ACHXWD_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR7621515.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="ACHXWD_002632"
     CDS             complement(8252..9769)
                     /gene="lysS"
                     /locus_tag="ACHXWD_002632"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR7621516.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="ACHXWD_002633"
     CDS             complement(10006..11463)
                     /gene="dtpC"
                     /locus_tag="ACHXWD_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR7621517.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11522..>11777)
                     /locus_tag="ACHXWD_002634"
     CDS             complement(11522..>11777)
                     /locus_tag="ACHXWD_002634"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR7621518.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3042 a         2856 c         2892 g         2987 t
ORIGIN      
        1 gcgccgtagc tggcggcaat gaaagcgagc gaggaatatc accgtcgtta atgcgccaag
       61 cagcgcgcag aacatatcgg attgcgtgtc ccactggtcg ccctgggtgc cgagaaaatc
      121 atccgctccc tggcccattg ccagcgccgc ccaccactcg attaattcat acatcgcgct
      181 tatcgccagc gccacgcagc agactaggaa cgccaccatt ttacgtccgc gcacgtacat
      241 cccgcgcacg agaatttctc gtgccaccag tgcaggcacc agcccctgga aaaaatgccc
      301 cagcttgtca tacggattac ggctcaaccc cagccattcc tgcacctcaa aaccgatggg
      361 gactttcgcg taggtgtatt gcccgccgac catcaggatg atggcgtgaa agaaaatgag
      421 cgtatagagc agcggcgtta acggataacg tttagcggtg gcaagcagta gcggcacgac
      481 aataatcacc ggtgtcactt ccatcagcca ggtgagtttg tcatgggccg aaatgccggt
      541 ataaatcagg attaacgtta gcgccagtgc gccggtgtta agaattaacg gtttgagtgt
      601 gcgggtcatg gtgagttcgc aagtcaggga aaatactgac tattcaccag caagcatgaa
      661 attacaatcg caaaagaggt ggcccagggg gatcacctgg cagcatgctg ccaggcgctg
      721 ggccgaagag gttacttagt gcagttcgcg cactgtttgt tgacgatttg ctggaagaag
      781 tcgttacctt tgtcatcgac caggataaac gccgggaaat cttctacttc gattttccag
      841 atagcttcca tacccagctc cggataagcg acgcattcca gatgcttgat gctctgctgc
      901 gccagtaccg ccgccggacc gccgatgcta ccgaggtaga agccgccgtg tttatgacac
      961 gcgtcggtaa cctgctggct gcggttgcct ttcgccagca tgatcatgct gccgccgtgg
     1021 gattgcagca gatccacata ggagtccata cgacctgcgg tggttgggcc aagtgaacct
     1081 gatggataac cggcaggagt tttcgccgga cccgcgtagt agatcgggtg atctttgatg
     1141 tactgcggaa gttctttacc ggcgtcaatc agctctttca gcttggcgtg tgcaatatct
     1201 cgacccacaa taatggtgcc agtgagcgac aaacgagtgg aaaccggata ttgcgaaagc
     1261 tgggcgagga tctctttcat cgggcggtta aggtcgacct tcaccgcttc gccttcaccg
     1321 gcctgacgca gttcttgtgg aatgtactga cctggattgt gttccagttt ttcgatccag
     1381 ataccttcgc ggttgatttt cgctttaatg ttacggtcag cggagcagga gacgcccatg
     1441 cctaccgggc aggatgcgcc gtgacgtggc agacggataa cgcgaatgtc gtgcgcgaag
     1501 tatttaccgc caaactgcgc gccaagaccg agtttctggg cctcttccag cagttcctgt
     1561 tccagctgga catcgcggaa cgcctgacca tgttcgttcc cttccgtcgg cagttcatcg
     1621 taatagtgag cgcttgctaa tttgacggtt ttcaggttgg tttccgcaga cgtaccgcca
     1681 atcacaaacg cgatatggta cggcgggcag gctgcagtac cgagggtacg cattttctcg
     1741 acgaggaagt ttttcagttt gccgggagtc agcagggctt tggtttcctg gtagagatac
     1801 gttttgttgg cagagccgcc gcctttcgca acgcaaagga atttgtactc atcgccatct
     1861 accgcgtaca ggtcgatttg cgcaggcagg ttagtgccgg tgttgacctc tttgtacatg
     1921 tccagcgccg cgttttgcga gtagcgcagg ttatcttcga tataggtgtt atagacgcct
     1981 ttcgacagcg cttcttcatc accgccaccg gtccacacgc gctggccttt tttaccgacg
     2041 atgatcgcgg tgccggtatc ctggcaggtc ggcagcacgc ctttggcggc gatttcggag
     2101 tttcttaaga attgcagtgc cacgtacttg tcgttttcgc tggcttctgg atcgtgaaga
     2161 atagccgcaa cctgtttctg gtgtgccgga cggagcatga aagaagcgtc gtgaaaggct
     2221 tgctgcgcca gcagggtcag ggcttctggt tccactttca ggatggtttc gccgtcgaag
     2281 tcggcaacgc taacgtaatc ggaagtgagt agatagtatt cggtattgtc tttccccatc
     2341 gggaaaggtg cctggtagat aaagggtttg tttgacatag cttccagcct gtaactctgt
     2401 atttgttatt cgaaaatggc gtgccgcgta gtcacgggcg gcacgcaaag tgcatttata
     2461 agaacccgta catcgcggcg aagatccagc cgaagacgca cgatacgctc acaccaatca
     2521 accccggcag aataaagctg tggttgatga cgaagcgacc gatgtgggtg gtgccggaac
     2581 ggtcaaactg aatcgctgcc agatcgctcg gataagtcgg caggatgtaa taaccgtagc
     2641 aagccggtgc tgaagccacg atgtatgccg gatcaacgcc gatcgccagc gcgaccggaa
     2701 caatcgccgc cagcgccgca gcctgagagt ttacaaactt ggaaaccagc agcagaacaa
     2761 tggcataggc ccacggatac tctttcacca tttcacccag tacgccctga atttcagaca
     2821 tatgcgcacc gaacatggtt tctgccatcc atgcgatacc gtacaccgcc acgatggcga
     2881 tcataccgga acggaagact tcgttttttg agatagacgc gggattggtt ttggtcagga
     2941 taataatcag cgccccggtc agcagcataa acatctgaat aaccagtacc atcgacagcg
     3001 gtttgccgcc gaaggatgga cgcaggtccg aatcagcacc aagaagggcg actacagcga
     3061 ttgccccgag gaaaatccac attgccagcc agttgctttt cggcagtttt ttgtccagca
     3121 gcgtcgcggt atcaccgtaa acatactcac ggttttccgg tacggagatg aatttctgga
     3181 actcttcgtc tttatccaga tctttaccac ggaaccagct gaagataccg atcgccagga
     3241 taccgattaa cgtcgatgga atggtgattg ccagcaggtc gaggaactca agatggcgac
     3301 catcaaaggt gacattaccc agcattgcaa ccagagacac gaccgcaacc gacaccggac
     3361 tggcgatgat ccccatctgt gcaccgatag aacttgccgc catcggacgt tccggacgga
     3421 tgttgttctt aatggcgacg tcgtagatga tcggcagaat ggtgtaaacc acatgacccg
     3481 taccgcaaag aatggtcagc gtacaggtca caaacggcgc gacaattgag acatatttcg
     3541 ggttgcggcg cagcagcttc tcggcaattt gcagcatgac atcaagaccg cccgaagctt
     3601 gcaaggtcgc cgatgccgcc accaccgcaa tgataaccag catgacatca accggtggtt
     3661 tacctggctg aaggtggaag acgaagacca gaatgaccag accgataccg cctaataaac
     3721 ccagcgcgat accacccttt ctggcaccat aaaacagaca tatcagtatt atgataagtt
     3781 ggatagtaaa taacatgtgt gaaccctcgc gataatccta tttaaatttt tgctgaatag
     3841 atcacagtca cgttctgttt tgtatgaact gttttcagag cctgactaaa tatccgtttg
     3901 gtcgttaccg gctttagcaa atacctcaca gtgaatattg gctgattaaa gcagcggcaa
     3961 atttatgcac tgtgataagc ggctttttca gagagaaaga ctctctggcg atgggtttat
     4021 agtttgtact gcgcagatgt taatgttttg tttgctgtta tcttattgat atttatcggt
     4081 ctattttttg ttctcagggc agatgtcatt aggtttatag attaatctga tctacccatt
     4141 tgtgggtaaa aatacacata atgcgggtga cataatagtt aattaacttt tgttagcgtt
     4201 ttgaaattaa aaacaccgtt cacctgaaga gatattaatt tttagcgatg atgcagggat
     4261 aattatattt gatctggcac aagttttact gatgaaggat gtaacttgtg ccaggggtat
     4321 tttgcattac ggtaataatt attacttaca gataaccagc gatccgttat tggcaatatt
     4381 gtttcagtag tgagtagtgt tctgcctgaa tacggtaacg gtaaactgga cgccccgtga
     4441 cgccataatg gatactggtg aacaagatgt ggcagttgac cagccagatg aggtatttac
     4501 ggcaggaaac acgcgaaatg ttaacctcgt tggctagctc gtcggttgaa aattcatagt
     4561 cctgatgcgc gtcaatccac tggcacagtg tgcgtaacgt ctgcggcgtt aagccttttg
     4621 gcaagcgacg aggatcctgt tcgttggagc tgctgccgtg gattagctga tcaagctcgg
     4681 cctggtcata atactgatgt ttttccagcg ccattttctt ttgccgccag tcggtgagcg
     4741 cctcttcaaa gcgggaagcc tggaagggtt tgatcaggta atccacgaca ccgtaatgca
     4801 gcgaatcttt aatggttgcc gcatcggctg cggaggagat gacaatcaca tcacttttgc
     4861 aacgcgcgtt atgcaggaca ggcagtaaat cgagcccgtt ctctttttgc atatagatat
     4921 cgagcaatat caggtcgata ggcgtatcgc tattgaagat aatctctttg gctttctcca
     4981 gcgtcgaggc tgttccacag cattgaaagc ctgggatttg tgctacgtat cggcgattca
     5041 gctccgcgac cattgcgtcg tcatcgataa ttaatacatt gatcatctgt tcgacctctc
     5101 cccgtcccag ggtatctgga caaaaaattg tgtgaaaatc ccgggttccg attccacggc
     5161 gatgctgccg ccgagatttt ctacctgttg tttgacaagt gctaaaccga cgcctcgctc
     5221 gcttcctttt gtcgagacac ctttgtcaaa aatgtgatcg attttgtcgg gtgcgatccc
     5281 cggtccatca tcgttaactt cgcagtgcag ccagccgtga cggtagtgca atgttacgct
     5341 aatttcgcca ccgggttccg gccctaatgc ttccagcgcg ttttctatca gatttcccaa
     5401 tgtggtaatc agcgtcgcga cctggtcctc actgccgctg tctggcagct ggctttcact
     5461 gtttaaaatc agcgtatggc ctaaatcggt cgcgcgatta atcttgctga ttaaaaaacc
     5521 agcgataacc ggagatttga tcttacccag cagagagcca atctcttcct gatagttatt
     5581 ggctgttttt agaatgtaat cttccaactg cttataactc ttcagatgca ataatccgag
     5641 aatcacgtgc aatttattca taaattcgtg ggatcgttca cgaagtgcgt cggcatagtt
     5701 gaccagaccg tcgagtcgct gcatcagttt acgtacttcg gttttgtccc tgaaggttga
     5761 aatggcaccg atgataacgc cattactgcg caccggaacg gtgttgatca gtaatagccg
     5821 gtctttaatc gtaatctctt cgtcgcggcg cggcgtaccg tcgcgtaaca cttccgagac
     5881 atctaccacc tgtgaccatg agtggctgag cgtcgacagt ttctcatcgt cctgcgactt
     5941 acggtaattc agcaattctt gtgcggcatc gttgatcagc gtgacctcgc cgcgatcgtc
     6001 cacggcaacg acgccttctt tgatagactg caacatggcc tggcgttgct caaacagcgt
     6061 ggagatttcg tagggttcca ggccgaaaag gatttttttc agtaccttaa ccagaatgca
     6121 ggtgccaatc agtccgacca gcatgccaaa taataccgac cagataatgc tccagcgact
     6181 gtcattgatt tgttgggtca cgcggcttaa ctcaaggccg atcgccacca cgccaatttg
     6241 tttatgattt tcatcgtaga tgggggtaaa tacgcgtaaa gcctgcgcca gaaaaccgcg
     6301 attgatagcg acattttctt tgccattcag cgctttaagg atgtcatcac ctttaaatgg
     6361 ctgaccaata cgctgggctt caggatgcga gtagcgaaga ctttgcatat cggtaacgac
     6421 aataaacagc agatcgttgc gtttgcgcac ggcttccgcg atggcctgga tgccgctctc
     6481 ctgcggtttt ttctgcaagc cctgacggat ttccggcgag ttggcgaggg tacgcgccac
     6541 tgccagtgcc ttgttggcta gcccgtctcg cgtcatatca ctgatttgcg agaagtaaat
     6601 cagatgcacc accaatagca ccgagaacag taccgcactg accattaaga tcactgtggt
     6661 actcaatttc atcggacgtt tgcgtaacat gcggtagggc aatgaatgtc tcatcagctt
     6721 ccttgtgtga caaatttctt aaacattatc tctgatgagg tgggtaattc aaagggagta
     6781 agaatgattg gctatatagg ggaagagatt ctggcaacgg aaactgccag tgctgtatga
     6841 agattccagg gctatgctta tagcgataat catactgatg agagagggaa agtcatggat
     6901 caggcgctac tggacgaggg ttatcgctgt tataccggcg aaaagatcga tgtctatttc
     6961 aacactgcga tatgtcagca ttctggcaat tgcgtacgtg gcaacggcaa gttatttaat
     7021 ctcaaacgaa agccgtggat catgccggat gaagtcgacg tcgccactgt ggttaaagtg
     7081 attgatacgt gcccgagcgg cgcgctgaaa taccgtcata aataagcgag ggtaaaatgg
     7141 aaatacgcga aggccacaat aaattttaca ttaatgacga acaaggcaag caaatcgctg
     7201 aaattgtctt tgtgccgact ggagagaatt tagcgattat cgaacatacc gatgtcgatg
     7261 aaagcctgaa agggcagggg attggtaaac agctggttgc aaaagtcgtg gaaaaaatgc
     7321 gtcgggaaaa acgaaaaatt atcccactat gcccatttgc gaaacacgaa tttgataaaa
     7381 cgcgggagta tgatgatatt cgcagttgat gggagagtac agagtcacga tatttttcat
     7441 gctctccgcg gagtgatgca ggagagcatc tgaagggtag ggggatgcac aaagaatggg
     7501 cagagagcgc gtttttttgt cccaagtcat cccctttact gagcaaaaaa aagaatatct
     7561 cctatatgag aatcatcaat cggggttatc aagttttgcg tccccagagc attcaatatt
     7621 gataggagtc atattatgga aggtaaaaac aagttcaata cttatgttgt ttcttttgat
     7681 tatccatcat cttattcctc agtgttctta aggttaagat cattgatgta tgatatgaat
     7741 ttctcttcta tcgtggctga tgaatatggg ataccacgac aattgaatga aaattccttc
     7801 gcaataacga catcgttagc cgcaagtgaa atcgaagatt taatcaggct taaatgctta
     7861 gacttaccgg atattgattt tgacctcaac attatgacag ttgatgacta tttccgtcag
     7921 ttttacaagt agccaggaag gataagaaga acataaaaaa tggcactatt ctctaaaata
     7981 ttaatttttt atgtgattgg tgtgaacata tcctttgtca ttatctggtt tatctcacat
     8041 gagaaaacac atattcgttt acttagtgca ttcctggtcg gaataacctg gccaatgagt
     8101 ctgcctgtgg cattactttt ttctctcttt taggagctgt agttgagtgg acccgctggt
     8161 tatcgtgaaa cgaagcgtct ctcctcaaaa ctggatttgc ggaacgacga acgaagcagt
     8221 cgcctgactg cttcgttcat taaagtgaaa tttatttctg tgggcgcatc gccgggaaga
     8281 gaataacgtc gcgaatagta tgactgtttg taaacagcat aatcattcgg tcgataccaa
     8341 tacccagacc agcggttggc ggcagaccat attccagcgc agttacgtaa tcttcgtcat
     8401 agaacatggc ttcgtcgtca cctgcagctt tagcgttaac ctgttcctgg aaacgttcag
     8461 cctgatcttc tgcgtcgttt aattcgctaa aaccattacc gatttcacga ccaccgatga
     8521 agaattcaaa acggtcggtg atttccgggt taacatcatt acggcgcgcc agcggtgaca
     8581 cttctgccgg atattccgta ataaaggttg gctgaatcag atgtgcttct gccacttcat
     8641 caaagatctc cgtgataata cgtcccagcc cccagctttt ctctaccgta attccgatag
     8701 attcagctaa tgctttagca gcatcaaaat tatccaggtc ggccatatcg gtttctggac
     8761 gatatttttt gattgcttcg cgcatggtga gtttttcaaa cggtttgccg aaatcaaaca
     8821 catgctcgcc ataagtgact ttagtggtac ccagaacctc ttgtgccagg gtgcggaaca
     8881 gtgactctgt cagttcaatc aaatcgtggt aatccgcata cgccatgtag agttccatca
     8941 ttgtgaactc aggattatgg cgaacagaaa taccttcatt acggaagtta cggttgattt
     9001 cgaatacccg ttcaaaaccg cctacaacca gacgtttcag atacagctcc ggcgcgatac
     9061 gcagatacat atctaaatcc agagcattat gatgggtaat aaacgggcga gcagatgccc
     9121 cacctggaat tacctgcatc atcggggttt ctacttccat aaagccgcgc gcgaccatga
     9181 actgacggat agcggccaga atttttgaac ggacaacaaa cgtttgacgg gatttatcgt
     9241 tagcgatgag gtccagataa cgttgacgat aacggacttc ctgatcctgc agaccatgga
     9301 atttatctgg taaaggacgt agtgctttag tcagcaggcg caactcagta cagtgaatgg
     9361 aaagctcacc cgtttgcgtc ttaaacagcg taccgcgggc accgataatg tcacccagat
     9421 cccatttttt aaactgatcg ttataaacac cttctggcag gctatctctt gcaacgtaca
     9481 gttgaatacg gccaccgaca tcctgcaacg ttacaaagga ggctttcccc atgatacgac
     9541 gggtcatcat tcggccagca accgagactt caatgtttaa ggattccagt tcctggttat
     9601 tcttcgcatc aaactcttcg tgcaactggt cagaggtatg gtcgcggcga aaatcattgg
     9661 gaaacgccac accttgctga cgtagtgccg ccagtttttc gcggcgattt ctcagttcat
     9721 cgttaaaatc aatagcctca ttggctcccc gtgtttcttg ttcagacatt ttggttcctc
     9781 taaatccagc tttcaattta gcgtagataa agagacagat cggtacactg tattatctgc
     9841 ctcgactatt aataactcag tttatcttat cgaaactaac gcttacctca agttgatatc
     9901 actaataaat aaccatcaaa atggtttaaa tgacttatta atctcgattt actagtgtga
     9961 ttaactgaaa gttaatttat tcataaccaa ttgtttttac gacagttaat cgttgctctc
    10021 ctgtatcata ttcgtcggcg tgctaaacag aaaacgggta gcaaaggcca atacgacgat
    10081 gatcgcgaca caagccaacg tccactctcc catctgagaa aagaatcgct ggtaagctgc
    10141 aattaccata ccgctaattt gcgactctgt cgtctgctgt gccacgacgc ctgccagcca
    10201 gttggcgacc gcgcctgttg ccagcatata aataccggtt aatacgccag acattttcag
    10261 acgcgtgatt tgcgcaatcg ccaccgggtc aataaagagt tctgcaaagc ccattagcgc
    10321 caacccggat atcatcacgc ccattgacgc ttgaccgtca gccgctgcat gtcgggcatc
    10381 aaatgccaac aacataaagc cacaagccat cagcagtaag ccaaaggcaa acttcagcca
    10441 gacgcgcaat gttgagttgc cgcggctttc tggcgacgcc agccaggcca gtacaacccc
    10501 agcgagcatc actgcaattg cattcaccga ctggaatagt gctgtaggta cttcaatatt
    10561 gaatgcctga cgattcacaa agcgatcgat aaacaagctg atggtactgc cgccctgttg
    10621 tgccagtacc cagaacaacg tcccgacaaa catcaacaat acaatttgcc aaagagcacg
    10681 gcggtgttcg gggaatttga tcatcatgcg ggcaatgatt tgtgcggcaa tgaggcaaac
    10741 gatcgccagc aaatatccgg accagtcgtt ctccagcagc agggtaaaaa atactggggc
    10801 taaacagagc atcaccacta accagctcca tactggtaag gcaaatttga cgctagtgag
    10861 cgctttttta tccatactac gcgtggactg gaaatgacga tgaccgctta agaaaatcaa
    10921 caaaccgata aacatgccgc cacccgcaag ggcaaagcca acatgccatc cataccactg
    10981 agcagccagg ccgcaggcga ttggggcggc gatagaaccg atattgcccg cagcatacag
    11041 cagcgaaaaa ccgccatcac gtcgatgatc gttctcgtcg tagagctcgc caagcaaaca
    11101 gctgatgttt gatttgaata aaccgtagcc acaaataatg attgccagcg ccagatacag
    11161 gctaaaggtt gaatttgtat caatgcccag caccacatgg ccaagggtca ttaacagcgc
    11221 gccggcaatc actgcagtgc ggttgccgag caggcggtcg gcaagccagc cgccgagaat
    11281 aggggtaacg taaaccagag aagcatatgc gctgaacagg ctgatggcat ggttatcatc
    11341 aaaaccaagc tgatgggtga gatagagaat gagtaaggca cgcatgccgt aaaaactgaa
    11401 gtactcccag atttggatcg ccacgatata gtatatcgcg cgcggctgtg agggtgtttt
    11461 catgtgttct ccttatgagc aaaaaaggga agtggcaagc cacttccctt gtacgagcta
    11521 attatttttt gctttcttct ttcaatacct taacggtata gcggccatca gcctgacggt
    11581 atgcaccgtg aatatcggtt tcaaatcccg gatagtgagt gccgatttca cacagcatct
    11641 gcaggaactc cagaaccgga cggctttctt cggtgatcat ttcacccggc attaccagag
    11701 gaactcccgg cggatacgga aggatcatat tggcgttaat acgacctacc atttcgtcga
    11761 ggtaaacttc ttcggtc
//
LOCUS       DAZSBF010000121        11770 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZSBF010000121 DAZSBF010000000
VERSION     DAZSBF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.122"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            89..229
                     /locus_tag="ACHXWD_002635"
     CDS             89..229
                     /locus_tag="ACHXWD_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR7621519.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            232..399
                     /gene="yciX"
                     /locus_tag="ACHXWD_002636"
     CDS             232..399
                     /gene="yciX"
                     /locus_tag="ACHXWD_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR7621520.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            513..608
                     /gene="ymiC"
                     /locus_tag="ACHXWD_002637"
     CDS             513..608
                     /gene="ymiC"
                     /locus_tag="ACHXWD_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR7621521.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            772..3447
                     /gene="acnA"
                     /locus_tag="ACHXWD_002638"
     CDS             772..3447
                     /gene="acnA"
                     /locus_tag="ACHXWD_002638"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR7621522.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(3511..4101)
                     /gene="ribA"
                     /locus_tag="ACHXWD_002639"
     CDS             complement(3511..4101)
                     /gene="ribA"
                     /locus_tag="ACHXWD_002639"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR7621523.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            4271..5035
                     /gene="pgpB"
                     /locus_tag="ACHXWD_002640"
     CDS             4271..5035
                     /gene="pgpB"
                     /locus_tag="ACHXWD_002640"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR7621524.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            5184..5492
                     /gene="lapA"
                     /locus_tag="ACHXWD_002641"
     CDS             5184..5492
                     /gene="lapA"
                     /locus_tag="ACHXWD_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR7621525.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            5499..6668
                     /gene="lapB"
                     /locus_tag="ACHXWD_002642"
     CDS             5499..6668
                     /gene="lapB"
                     /locus_tag="ACHXWD_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR7621526.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            6861..7598
                     /gene="pyrF"
                     /locus_tag="ACHXWD_002643"
     CDS             6861..7598
                     /gene="pyrF"
                     /locus_tag="ACHXWD_002643"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR7621527.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            7598..7924
                     /gene="yciH"
                     /locus_tag="ACHXWD_002644"
     CDS             7598..7924
                     /gene="yciH"
                     /locus_tag="ACHXWD_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR7621528.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(8050..8268)
                     /gene="osmB"
                     /locus_tag="ACHXWD_002645"
     CDS             complement(8050..8268)
                     /gene="osmB"
                     /locus_tag="ACHXWD_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR7621529.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(8537..9286)
                     /locus_tag="ACHXWD_002646"
     CDS             complement(8537..9286)
                     /locus_tag="ACHXWD_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR7621530.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(9376..9549)
                     /gene="yciZ"
                     /locus_tag="ACHXWD_002647"
     CDS             complement(9376..9549)
                     /gene="yciZ"
                     /locus_tag="ACHXWD_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR7621531.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            9586..9714
                     /gene="ymiD"
                     /locus_tag="ACHXWD_002648"
     CDS             9586..9714
                     /gene="ymiD"
                     /locus_tag="ACHXWD_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR7621532.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(9696..11686)
                     /gene="pdeR"
                     /locus_tag="ACHXWD_002649"
                     /pseudo
     CDS             complement(9696..11686)
                     /gene="pdeR"
                     /locus_tag="ACHXWD_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         2991 a         2779 c         2996 g         3004 t
ORIGIN      
        1 tttgactaaa gtttctttag gatttgtctc atcgtatgat taattacacc aatcgttcaa
       61 cgtcttgctg ataagtgatg gcgattatat gaggttagca atgccttctg gaaatcagga
      121 accccgccgc gatcctgaat taaaacgtaa agcctggctg gcggtttttc ttggttctgc
      181 acttttctgg gtggttgtcg cactgctgat ttggaaagtg tggggataac tatggtcggt
      241 caggagcaac tggagtcgtc accattatgc cagcatagtg acaatgaaac agaaacgaaa
      301 cgggaatgtt ccgtcgttat tccagacgac tggcaactaa catcgcagca gcaagccttt
      361 atagaactgt ttgctgaaga tgatcagccg aaacaataat tatcatcatt cttattaccc
      421 atttttaatg aattaaaggg cttttaatac accgcagcaa taacagcttg agttatctca
      481 acacaaaata ataaccgtta agggtgtagc ctatgatcaa cacaaatatg aaatattggt
      541 cctggatggg cgcgttttct ctgtcgatgc tcttctgggc cgaactcctc tggatcatta
      601 ctcactgatc cttgaccccg ctgcggcggg gttgtcattt gctttgccac aaggtttctc
      661 ctcttttatc aatttgggtt gttatcaaat cgttacgcga tgtttgtgtt atctttaata
      721 ttcaccctga agagaatcag ggcttcgcaa ccctgtcatt aaggaggagc tatgtcgtca
      781 accctacgag aagccagtaa ggacacgttg caggccaaag ataaaactta ccactactac
      841 agcctgccgc ttgctgctaa atcactgggc gatatcaccc gtctacccaa gtcactcaaa
      901 gttttgctcg aaaacctgct gcgctggcag gatggtaact cggttaccga agaggatatc
      961 cacgcgctgg caggatggct gaaaaatgcc catgctgacc gtgaaattgc ctaccgcccg
     1021 gcaagggtgt tgatgcagga ctttaccggc gtacctgccg ttgttgatct ggcggcaatg
     1081 cgcgaagcgg ttaaacgcct cggcggcgat actgcaaagg ttaacccgct ctcaccggtc
     1141 gacctggtca ttgaccactc ggtgaccgtc gatcgttttg gtgatgatga ggcatttgaa
     1201 gaaaacgtac gcctggaaat ggagcgcaac cacgaacgtt atgtgttcct gaaatgggga
     1261 aagcaagcgt tcagtcggtt tagcgtcgtg ccgccaggca caggcatttg ccatcaggtt
     1321 aacctcgaat atctcggcaa agcagtgtgg agtgaattgc aggacggtga atggattgct
     1381 tatccggata cactcgttgg tactgactcg cacaccacta tgatcaacgg ccttggcgtg
     1441 ctggggtggg gcgtgggtgg gatcgaagca gaagccgcaa tgttaggcca gccggtttcc
     1501 atgcttatcc cggacgtagt gggctttaaa cttaccggta aattacgtga aggtattacc
     1561 gccacagacc tggttctcac tgttacccaa atgctgcgca aacatggcgt ggtggagaaa
     1621 ttcgtcgaat tttatggtga tggtctggat tcactaccgc tggcggatcg cgccaccatt
     1681 gccaatatgt cgccagaata tggtgccacc tgtggcttct tcccaatcga tgctgtaacc
     1741 ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg ttgagttggt cgaaaaatat
     1801 gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac cgatttttac cagtacgtta
     1861 gaactggata tgaatgacgt tgaagcgagc ctggcagggc caaaacgccc acaggatcgc
     1921 gttgcactgc ccgatgtacc aaaagtattt gccgccagta acgaactgga agtgaatgcc
     1981 acgcataaag atcgccagcc ggtcgattat gttatgaacg gacatcagta tcagttacct
     2041 gatggcgctg tggtcattgc tgcgataacc tcgtgcacca acacctctaa cccaagtgtg
     2101 ctgatggctg caggcttgct ggcgaaaaaa gccgtaattc ttggcctcaa gcggcaaccg
     2161 tgggtcaaag cgtcgctggc accaggctcg aaagttgttt ctgactatct ggcaaaagcg
     2221 aaactgacac cgtttctcga cgaactgggg tttaaccttg tgggatacgg ttgtactacc
     2281 tgtattggta actctgggcc gctgcccgat cctatcgaaa cggcaatcaa aaaaggcgat
     2341 ttaaccgtcg gtgcagtgct gtccggcaac cgtaactttg aaggccgtat ccatccgctg
     2401 gttaaaacca actggctggc ctcgccgccg ctggtggttg cctatgcgct ggcgggaaat
     2461 atgaatatca acctggcttc tgagcctatt ggtcatgatc gtaaaggcga tccggtttat
     2521 ctgaaagata tctggccatc ggcacaagaa attgcccgtg cggtagaaca agtctccaca
     2581 gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca cagcagagtg gaaggaaatt
     2641 aacgtcacac gatccgatac ctacggttgg caggaggact caacctatat tcgcttatcg
     2701 cctttctttg atgaaatgca ggcaacacca gcaccagtgg aagatattca cggtgcgcgg
     2761 atcctcgcaa tgctggggga ttcagtcacc actgaccata tctctccggc gggcagtatt
     2821 aagcccgaca gcccagcggg tcgatatcta caaggtcggg gtgttgagcg aaaagacttt
     2881 aactcctacg gttcgcggcg tggtaaccat gaagtgatga tgcgcggcac cttcgccaat
     2941 attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg ggatgacgcg gcatttacct
     3001 gacagcgacg tagtctctat ttatgatgct gcgatgcgct ataagcagga acaaacgccg
     3061 ctggcggtga ttgccgggaa agagtatggt tcaggctcaa gccgtgactg ggcggcaaaa
     3121 ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat cgtttgaacg aattcaccgt
     3181 tcgaatttaa ttggcatggg catcctgccg ctggaatttc cgcaaggcgt aacgcgtaaa
     3241 acgttaggac taaccgggga agagaagatt gatattggcg atctgcaaaa tctacaaccc
     3301 ggtgcgacgg ttccggtgac gcttacgcgc gcggatggta gccaggaggt cgtaccctgc
     3361 cgttgtcgta tcgacaccgc gacggagttg acctactacc agaacgacgg cattttgcat
     3421 tatgtcattc gtaatatgct gaagtaacaa atatttgctt gccggttatt ttgcttccgg
     3481 caagcaaatt aattccacaa tgcgagaggg ttatttgttc agcaaatgac ccattttctc
     3541 ggctttggta tcgagataat gttcgttatt ggggttacga cctacaatca atggtacgcg
     3601 ttcaacaata ttaatccctg cttcggtcag aatttcgact tttttcgggt tattggttaa
     3661 caagcggact tcattgacgc caaggagttt gaacatatca gcgcaaagag tgaagtcgcg
     3721 ctcatcagcg gcgaagccta actgatggtt agcctctacg gtatcgtaac cttgatcctg
     3781 cagtgcgtaa gcgcggattt tattcagcag accaatgtta cgaccttcct gacggtgata
     3841 cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat gccgcttcga gctggaagcc
     3901 acaatcgcag cgcaagctga acagggcgtc accggtcaga cattcggaat ggacgcgcgc
     3961 aagtaccggg gtatgcccgg aaatatcgcc atagactagc gcgacatgat cgtgtccggt
     4021 tgccagttct tcaaatccca ccatcaggaa atcgccccat ggggttggca gtttggcttc
     4081 tgccacacgt ttaagctgca tgaaattctc cagataatgc tggttctgta ttggcttatt
     4141 ttgccataac gagaaggggt tcacctaatc acgacgcgtc gatcgttcac ggaatggcac
     4201 aaatctgtca atttttcctg gaactggcgt tttcagttat gattgtggga cttatcaaaa
     4261 aggagaggcc atgcgttcga ttgccagacg taccgcagtg ggagctgcac tattgcttgt
     4321 catgccagta gccgtatgga tttctggctg gcgttggcaa cctggagaac aaagttggct
     4381 actaaaagcg gctttttggg ttactgaaac tgtcacccag ccctggggcg tcattacaca
     4441 tttgatttta ttcggctggt ttctctggtg tctgcgtttt tgcattaagg ctgcctttgt
     4501 attatttgcc attctggcgg ccgtaatcat tgtgggacaa ggcgttaaat cctggatcaa
     4561 agacaaagtc caggaaccac gaccttttgt tatctggctg gaaaaaacac atcatattcc
     4621 ggttgatgag ttctacactt taaagcgagc agaacgcgga aatctagtga aagaacagtt
     4681 ggctgaagag aaaaatatcc cacaatattt gcgttcacac tggcagaaag agacggggtt
     4741 tgcctttcct tccggtcaca cgatgtttgc tgccagttgg gcactgctgg ccgttggttt
     4801 gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg ctggtctggg caacgggagt
     4861 catgggaagc cgcctgctgc tcgggatgca ttggccacgc gatctggtag tagctacgtt
     4921 gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg caacgaattt gtgggccatt
     4981 aacaccacct gcggaagaaa atcgcgaaat agcgcaacga gaacaagaaa gttaacgctg
     5041 gttgattttc cgaatttagc ccttaaatca tcaacaatgc gcgtggatgc catttcgcag
     5101 acggcgcgaa aatggtactt taaagggcta ttgcggtaag ttgaccataa tttattcgct
     5161 ttaaccacat aacgggaagt aatgtgaaat atttactcat tttcttactg gtgttagcga
     5221 tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca acaggtgacg tttaattatc
     5281 tgttagcgca aggggagtac cgtatttcca cattgctggc ggtattgttt gctgcggggg
     5341 ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg agttcgtgtt tccctggcgc
     5401 gcgctgaacg taaaataaag cgactggaaa accagctttc acccgcgact gacgtggctg
     5461 tagtgccgca ctcgtcagcg gcgaaggaat aactttctat gctggagttg ttgtttctgc
     5521 ttttgcctgt agccgctgcc tatggctggt atatgggccg cagaagtgcg caacaaaaca
     5581 agcaagatga agccaaccgc ttgtcgcgtg attacgtagc gggggttaac ttcctgctta
     5641 gtaatcaaca ggataaagcg gtagacctgt ttctcgatat gcttaaagag gatacaggca
     5701 ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc gcgtggcgaa gttgatcgcg
     5761 ctattcgcat ccatcagacc ctaatggaaa gcgcctcact gacctatgaa cagcgtctgt
     5821 tggcgattca acaactgggg cgtgattaca tggccgccgg gttatacgac cgcgcggaag
     5881 acatgttcaa tcagctgacc gatgaaactg acttccgcat tggcgcgctg caacagttgc
     5941 tacaaatcta ccaggctacc agcgagtggc agaaagcaat tgatgttgcc gaacgcctgg
     6001 tgaagctggg taaagataaa cagcgcgtcg aaattgccca tttctactgt gagttagccc
     6061 tgcagcatat ggccagcgac gatctcgatc gtgccatgac cttgctaaaa aaaggggcgg
     6121 cggcagataa aaacagcgcc cgcgtatcca tcatgatggg acgcgtgttt atggcgaaag
     6181 gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat atcccaggac agagaactgg
     6241 tcagcgaaac gctggaaatg ttgcaaacct gctatcagca gttgggtaaa actgccgaat
     6301 gggcagaatt cctgcaacgc gcggtggaag agaacaccgg tgccgatgct gaattgatgc
     6361 ttgctgatat catcgaagcg cgcgacggta gtgaggccgc acaggtctat attacgcgcc
     6421 agcttcagcg tcatccgacc atgcgtgtgt tccataagtt aatggattac cacttaaatg
     6481 aagcggaaga agggcgtgcc aaagagagcc tgatggtgct gcgtgacatg gttggcgaaa
     6541 aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg ttttaccgca tacaccctct
     6601 actggcattg tccgtcttgt cgggcctggt caaccattaa accgattcgc ggtcttgatg
     6661 gcctgtaatt tttaaaaaaa tccgacttta gttacaacat actagttatt gatgttccat
     6721 tgtgctccgg caacgacgga gcagaaaaag cctgccaggg gaaaaatcgc aactgttaat
     6781 tttttatttc cacgggtaga atgctcgccg tttacctgtt tcgcgccact tccggtgccc
     6841 atcatcaaga aggtctggtc atgacgttaa ctgcttcatc ttcttcccgc gctgttacga
     6901 attctcctgt ggttgttgcc cttgattatc ataatcgtga tgacgcgctg tcctttgtcg
     6961 acaagatcga cccacgcgat tgtcgtctga aggtcggcaa agagatgttt acattgtttg
     7021 ggccacagtt tgtgcgcgaa cttcaacagc gtggttttga tatctttctt gacctgaaat
     7081 tccacgatat tcctaacact gcagcgcacg ctgtcgctgc tgcagctgac ttaggcgtgt
     7141 ggatggtgaa tgttcatgcc tctggtgggg cgcgtatgat gaccgcagcg cgtgaggcac
     7201 tggttccgtt tggcaaagat gcaccgcttt tgattgctgt gacagtgttg accagcatgg
     7261 aagccagcga cctggtcgat cttggcatga cactgtcacc tgcagattat gcagaacgtc
     7321 tggcggcact gacgcaaaaa tgtggccttg atggtgtggt gtgttctgct caggaagctg
     7381 tgcgctttaa acaggtattc ggtcaggagt tcaaactggt tacgccgggc attcgtccgc
     7441 aggggagtga agctggtgac cagcgccgca ttatgacgcc agaacaggcg ttgtcggctg
     7501 gtgttgatta tatggtgatt ggtcgcccgg taacgcaatc ggtagatcca gcgcagacgc
     7561 tgaaagcgat caacgcctct ttacagcgga gtgcatgatg agtgattcca acagccgtct
     7621 ggtctactca acggaaaccg gacgtattga tgagcccaaa gcggcccctg tacgccctaa
     7681 aggcgacggt gtggtgcgta ttcagcgtca gaccagtgga cgtaaaggta agggcgtttg
     7741 cctgattacc ggtgtcgatc tcgatgatgc cgaactgaca aaacttgcag cggaactgaa
     7801 gaaaaaatgc ggctgcggcg gagcagttaa agatggagtt attgaaatcc agggcgataa
     7861 gcgtgattta ttaaaatcac tacttgaagc gaaagggatg aaggtaaaac tcgcaggcgg
     7921 ttaacataaa aagccacgga tatatccgtg gctttcgaat attttactgt gcgtattatt
     7981 cagttatttc gtactgaaca gaatcaagcg tattataatt gcgagtcgat aatcgacatt
     8041 tatttacgat tatttaccga cctggtgacc aataacacca ccgacagctg caccacctaa
     8101 tgtacccaac gtactgccat cggtcagtac tgcaccgcct aatgcccctg cacccgcgcc
     8161 gattgcggtg ttgcggtccc gtttagacca gttagaacag gcactcagag acattgccaa
     8221 agtaattgcc agaacagccg cggtcatttt tttgctcgtt acaaacataa tactctctcc
     8281 tgaatttatg attcacggaa gtaagctctc tataactata atagctaaga ataagcctgg
     8341 tgaaattatt ccgtgaaatc tgctcgcgcg cagggttata tcacgcaggt gataatgact
     8401 tcctgttata tcgctgataa taattttata tcttaagcgt gttaataaca ggtaaatagt
     8461 cttaattatc aaccaggaat catcttagag cggatgattt gccaaactgc aaatcatccg
     8521 tagagaaggg aaatggttaa atgtcaacga cgtgaacggt gagtttactg tgctcaagat
     8581 gcatcagatc gctggcgcgt attttcgaat cggtaatcac gcgattgaat cgctcaacgg
     8641 gaccgatgga gtatgaatgt acagcaccaa atttcgagct gtcagtcagg actatcgctt
     8701 cgcactcttt ttccagcacg gcattgacca catcggtacg catcatatcg cgaccggtga
     8761 atccagtttc aggttgccag ccatcaatac caataaatgc tttgctgaaa tgcacctgtt
     8821 ggatgcactg gcgtgtcaac gggccaacca tactttcgct ttttttctgg tacacgccac
     8881 cgagcaaaat gacttcacaa ggcgcgtctt tcagcaaatg cgcgatgtag ctgctgaccg
     8941 tgatgatagt gacatttttc ttctgctccc ccagagtccg ggcgagcagg gcattgctgc
     9001 tgccattttc gataaagatg gtttcgcccg gttgaaccag tgacgcggca aactcggcga
     9061 gttcacgctt cagcgtatag ttgctcatca tacgggtttc gacgtcatca ctatcaagcg
     9121 aaactgcaaa gccatgtgca cggcggaggt aactcagttt ttcgagggtg ttgagatcct
     9181 ggcgaatggt aacttcagaa actccagtgg cttttgccag atcggttacg ctaacctgac
     9241 cctggtcaat gaccatctgt agaatagttt gttgtcggga attcatcgct gtaattctta
     9301 taacgttata atactaaata aaaaaatact gacgcgccgg gcatcctcaa ataggaaaac
     9361 ctgaaggcac gattgttaaa tctcccacgg tgccttcggg atgtctggcg tagattcagc
     9421 aacctggcgc aagagttctg ctttaagtat ctcgagatta tggatcgcag agtggtaatc
     9481 gcctgccaca aggatatcta acacaatatc aatacgttcg gcaacgcgct gggcatcaaa
     9541 ttcagacatc aaggacatcc ttatagcaaa agtggaaaat gaataatgca aaaattgccg
     9601 ctaaaagaga agtgtttaac agcaacggct aattatcatc caggaatacg atatataatg
     9661 acgggatata gcgctaagta tatatattca tctacttatg cgcgcttcag atagcgttta
     9721 taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa acaaaaatcc ttgccgctca
     9781 ttgatcccgt tcttggttaa aaaagcatct tcctttgcac tctctacacc ttcggcgatc
     9841 acctgaagat tcaatgcctg ggccacagcg acgatcgccc ggaccagtga ctgcgagaca
     9901 ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt tgatggcatc gatcggaaag
     9961 cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat catccagatg cacttgcgca
    10021 cctagttggc taaattgttg aataacagac agtgccagtt catcattctc aatcagacaa
    10081 ctctctgtca gttcaacatc tatagggcag tattcaaaat tgagttcctg gagaacctgt
    10141 ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg cagaaatatt taccgccact
    10201 cgcaggttta tgcctttatc ccgccacttt gccacctggc gtacgacatc gagaatcacc
    10261 caacggccta aaggcacaat tagccctgac tcttcggcgt aggaaatgaa gtccaacggt
    10321 ggaatcaacc cacgttcagg tgactgccaa cgtactagtg cttccagact gcgcacttcg
    10381 ccacgccagg tgattttcgg ttgatagtga ataaccaact gatcgttttc cagtgcttta
    10441 cgcaagttgg tatccagcca gagatattca aatacccgct gattcatttc tggagtaaaa
    10501 acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca ttgctgtgtc ggcgtgacga
    10561 ataatagtcg cgctgtccga accatgttcg ggagagagcg aaatacctac tgaacagctg
    10621 gtataaactt caattaaacc aatacgaaag gggaggcgta agcgggtcaa aattcgtgat
    10681 gccattgctt ccagcgcgct ttgtgaggta ttggatgcca gtaccagaaa ctcatcgcca
    10741 cctggacgcg ccaacacctg gtcatgttcg agacagctta aaatagccaa tgacacgtcg
    10801 cgtaataact ggtcaccaaa caaatgccca taggcgtcgt tgaccttttt aaaattatcc
    10861 aaatcaagat acacaacccc aactttattg ttatcagcct gattaatagc gtgatcgatt
    10921 aactcctgca ttgcgttacg attcggcagt ccggtgatac tgtcggtatt tgccagaata
    10981 cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg taccggaaca gattaaaaaa
    11041 atctcgtttt tgccactgcc gctgtggaca aatttatttt attgcgaaac agaaacagcc
    11101 gctggccttt acgtgttggt atccacagtt cgacttcata tgcattgccg cttcgaaaaa
    11161 atacacggtt attgcgcctg gatgccgcag cttcacgacg gctcataaac agtttaaaca
    11221 cgctttggcc aatgacgtcg tgttctttca acccagtgta atcttcacat aaccgattga
    11281 agcgttggat attcccccgg ctgtcgagaa tcactattgc ggaatgtgct tcagaaacta
    11341 cctgctcagc aaaggaaagc ccgtgtgata aatcacgagc aactgccggt gtgtcatgcc
    11401 atgcagacgc gttgccagcc cattcccgtt tattgatttt tcgtcctacc agatgcacag
    11461 aaagatcgct ctcatcgacg gtcagactca tcatcaggct ggaggtgatg accgtcattt
    11521 ccctgatgcg agcagcctgt tcggcagata actgcaaagt acgatcaggt tctgtggttt
    11581 cggtggtaaa aaagcgcaaa acatcgctgc tttccgtcaa ccgccagtat ggattgtgcg
    11641 atccgagaaa gttgtacaac gttgtggact ccctaacggt tttcatgatg catatcctcc
    11701 ggttaactgc ggagtccgcg gttaaataaa aggaacaaca tgacttacat gaaattaacg
    11761 gcggctaaac
//
LOCUS       DAZSBF010000122        11680 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZSBF010000122 DAZSBF010000000
VERSION     DAZSBF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.123"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..415
                     /locus_tag="ACHXWD_002650"
     CDS             <1..415
                     /locus_tag="ACHXWD_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7621533.1"
                     /translation="SMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSA
                     GNNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSC
                     FAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            626..949
                     /locus_tag="ACHXWD_002651"
     CDS             626..949
                     /locus_tag="ACHXWD_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR7621534.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            946..1776
                     /gene="amiD"
                     /locus_tag="ACHXWD_002652"
     CDS             946..1776
                     /gene="amiD"
                     /locus_tag="ACHXWD_002652"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR7621535.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1773..2786)
                     /locus_tag="ACHXWD_002653"
     CDS             complement(1773..2786)
                     /locus_tag="ACHXWD_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR7621536.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2885..4315)
                     /locus_tag="ACHXWD_002654"
     CDS             complement(2885..4315)
                     /locus_tag="ACHXWD_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR7621537.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4326..5327)
                     /gene="ltaE"
                     /locus_tag="ACHXWD_002655"
     CDS             complement(4326..5327)
                     /gene="ltaE"
                     /locus_tag="ACHXWD_002655"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR7621538.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5364..7082)
                     /gene="poxB"
                     /locus_tag="ACHXWD_002656"
     CDS             complement(5364..7082)
                     /gene="poxB"
                     /locus_tag="ACHXWD_002656"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR7621539.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7215..8183)
                     /gene="hcr"
                     /locus_tag="ACHXWD_002657"
     CDS             complement(7215..8183)
                     /gene="hcr"
                     /locus_tag="ACHXWD_002657"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR7621540.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8195..9847)
                     /gene="hcp"
                     /locus_tag="ACHXWD_002658"
                     /gene_synonym="priS"
     CDS             complement(8195..9847)
                     /gene="hcp"
                     /locus_tag="ACHXWD_002658"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR7621541.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9991..10890)
                     /gene="lysO"
                     /locus_tag="ACHXWD_002659"
     CDS             complement(9991..10890)
                     /gene="lysO"
                     /locus_tag="ACHXWD_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR7621542.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11385..>11680)
                     /locus_tag="ACHXWD_002660"
     CDS             complement(11385..>11680)
                     /locus_tag="ACHXWD_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR7621543.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         2852 a         3080 c         3094 g         2654 t
ORIGIN      
        1 tagtatgcat ggtgtcaaga taaaaccatg gttgccagta aaatggacaa aagagttaat
       61 aaatgattat ttgtatggct gtttactatc tctgtactct atctgtgcaa gggatatata
      121 taatatgagc gccgggaata acgtgaaggt tgctgcggat gctttccttg aaattatttt
      181 ctctcttaag aataaatatt gcattaaact attaagcgct caggaacggg cgtttattta
      241 cgaatttgca agaatgatct tcgcttatat taatgacaaa agcattgaga tcttattact
      301 ttcttgtttt gcggcgtctg accaaaaagc aatccagagg tatatacctc agtcacaaga
      361 tggggaagac tttaggagtc atttacaata taagttgcct ctttccgctc attaatgttg
      421 ttggtgcaac ataataagac aggtaagcgg taccctataa tgaaaccaat atcgggttat
      481 gcaacggata atcgtaaaga cttaagtggg tgtttttgac acaatgagat gatttcctaa
      541 actgatcaaa accttcttcc caacatggca tatcagttcg ataatgcttt agagatcgaa
      601 ggcttaacaa acaaggagat cgatcatgca attttctaca accccaactc tggaaggcca
      661 gaccatcgtt gaatattgcg gtgtggtgac cggcgaagcg attttaggtg ccaatatttt
      721 ccgtgatttc tttgccggta tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa
      781 agaactgcgt aaagcacggg agatcgcctt tgaggaatta ggctcccagg cgcgggcgct
      841 gggggccgat gccgtcgtcg gtattgatat cgactacgaa acggtcgggc aaaacggcag
      901 tatgctgatg gttagcgtca gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc
      961 tggctggtcg ctgccgctct gttattggca gggtgtgcag gcgaaaaagg cattgtcgag
     1021 aaagagggat atcagcttga tacccgacgc caggcgcagg cggcgtatcc gcgcattaaa
     1081 gtgctggtga tccactacac cgcagatgat tttgatagct cgctggcgac actgaccgat
     1141 aagcaggtca gctcgcatta tctggtccct gcggtaccac cgcgatacaa cggtaaaccg
     1201 cgcatctggc aactggtgcc ggaacaagaa ctggcctggc atgcggggat tagcgcctgg
     1261 cgcggggcaa cgcgccttaa cgacacctct attggcattg agctggaaaa ccgtggctgg
     1321 caaaaatcgg ccggagtgaa atattttgcc ccgtttgaac cgacacagat tcaggcgctt
     1381 attccactgg cgaaagatat tattgcccgt tatcacatca agccggaaaa cgtagtggca
     1441 catgcggata tcgcaccgca gcgcaaagac gatccggggc cattatttcc ctggcagcaa
     1501 ctggcgcagc aggggattgg tgcctggccg gatgcgcagc gggttaactt ttaccttgcc
     1561 gggcgcgcgc cgcacacacc ggtagatacc gcgtcattgc tggagctttt ggcgcgctac
     1621 ggttatgacg ttaaacctga tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc
     1681 cagatgcatt tccgcccgac gttatataac ggcgaagcgg atgcagaaac tcaggcgatt
     1741 gccgaagcat tgctggagaa atacgggcag gattagcgcg gcagttttcc gtggtcgcgt
     1801 agccaggcgg cagttttctc gataccttca tctagggtga tgatcggctg ataacctaac
     1861 tcttcctgcg cacgcgtaat atccagcgta aagtcaaaat tcaacttgga gacgccgtag
     1921 tgggtcagcg gcggctcttt tgctgacttg cggcctaaac gctccatgct gcgggcgatc
     1981 atatccagca tcgggtaggg gacggaacga atacgacagt caatattcaa ctcgtcgatc
     2041 agcttctgca cgatgctgcg cagtgtgcga tgctcgccgt tggtgatatt gtacacacgc
     2101 ccggaaggta gcttatcgca ggcttcctgg cttgccagcc acattgcgtg cacggtattt
     2161 tcatagtagg tcatatcgac cagcgcactg ccgccatgcg gtaacagaat actgccgtag
     2221 tggtgcatca tatgcgccag acggggaata aagactttat cgtgcggtcc gaacagactt
     2281 tgtgggcgca gaatagtaaa gcgcgtttgt ggattcgcct gcgaaagcat attgatcact
     2341 tcttcgctgg ctgctttgct gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct
     2401 tctttaatat cgcgatggtg gtgataatcg aagtacaggg agggggaaga gatatgaata
     2461 aagttacgca caccccaggc gacagcccat tcacccaggc ggcgagtggc gcgaacgtta
     2521 gccagatcga aagcctgttg tgtcccccag ggtgaggtaa agctggagca gtgccacagc
     2581 gtatcaatgc ccgcgagcat cactttagct tgtgatgaaa ccagctcggt cagatccgcc
     2641 ggaacaaact ctgcgcccat tttttccagc aatttgccca ttgcctcgtt gcgaccggtc
     2701 gctcgcacgc tgatgccttt ctggcataaa aactctaccg cgtttcgacc taagccgctg
     2761 gtggcgccgg taaccagtac cttcatatca atccactgtt gttgagaaaa tgacgtgcgc
     2821 attcttccgt gatttccccc atgatgcaat gggaaacatg aaagaataac gcaggttttg
     2881 tcgattaatc tgtgctttgt tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa
     2941 taaacagatg cgcggggatc atcaataacc agtaaaacag ccccggcata ccgtgcggat
     3001 gccagaaagc gcggacatcg atagtacgat agtcgccttt atcttccagg ctaaaacaca
     3061 gtcgtcccag ccccgacgct ttcatgccaa ataacaacgt aagttgtttt tccggttcaa
     3121 cgacaatcac tttccagcta tccaccgcat cgccagtctg taaatattcg cgctccgggc
     3181 ggcctttcgc cagcttatga ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa
     3241 tattgccaaa gaaataacgc tctttaccgc cgatttggtt cactacctgc cataaagcag
     3301 caaggctggc ggacgtttta acggtaaacc ccgcctgttt ggcaaaataa ccgtactccg
     3361 gtcgccagcg ggcaaaggcc tgagcgtcgt agccccagtc gctggagttg accagttttt
     3421 cctcctcttt caacgtgcta cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga
     3481 gtgcacgtag cgcggtatca tccgccagca gatcgtgttt cagcccctga atcaacgccc
     3541 tggcggtggt gggcggtacg gaagtaatca cattgagaaa ccacaccgaa atccagcggg
     3601 tggggagggg gatggggatc aaccagcggc gcttaccgct caccgccata aaatgttcaa
     3661 actgttgctg ataactgagc acctctggtc cggcggcttc gaagatgcgg tgttcgctgg
     3721 ccggatgatc taacaacgcc accagatagt gcagcaagtt ttccagcgcg atgggcgtgg
     3781 tgcgtgaacg tacccagcgt ggcggcgtta acactggcag gttgtagacc atatcgcgca
     3841 tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc ggcccgaagt tcggtcacag
     3901 gtacattcgc ttcacgaaga atgtccgccg tagcctgacg agcacgcaga tgatccgact
     3961 gctcatgtgg cggggcctgc aacgaactga gaaagattaa ttgcttaact ggtacttcac
     4021 gtagcgcatc gcggacgttg agagccacct ggcgctcctg agcgataaaa tcgccgcctt
     4081 cgcccatgct gtgcaccaga aaatagacgg tatcgatatc ctgcaacagg gccggaaggt
     4141 tatccggcca gctgagatcg actttatggc aactgacgtt tgccagttgc agctttgcaa
     4201 gcctgtcgac atgacgtgcc gccgccagga tctgatgccc ttgctggctg agtgtgcgca
     4261 ccagatgctg accaatgtag ccactggcac cgagaactaa aatgcgttgc ggcacgtctc
     4321 tctccttaac gcgccaggaa tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc
     4381 gagacgtcaa gatgcgtcac caggcggaca atcggcgagg cgttaatcag cacgtttctc
     4441 gctttcatgt attcgcctaa cgcggcagca ttttcttccc cgacgcgaac aaacagcata
     4501 ttagtgtcct gacgcatcac atccgcgcct gcttcacgca gttgctccgc catccaggcg
     4561 gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt tcagggcata catcccggcg
     4621 gcagccagaa tcccggactg acgcatcccg ccaccggcca ttttccgcca gcggatggca
     4681 cgtttaatgt aatcacgatt accgacgagt aatgaaccga ctggcgtccc aagacctttc
     4741 gacaggcaaa tggtgaacga atcacaatat tgcgtgatct ctttcagttc gcagccgtaa
     4801 gccaccacgg cattaaagat gcgcgcaccg tcaacatgca gcgccagatt gcgcttgcgg
     4861 gtaaattccc atgcttcttt caggtattca cgcggcagca ctttgccgtt gtgggtgttt
     4921 tctagactga gtaatttggt gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc
     4981 actttatcca gcggtagcgt gccgtcggca gccgcgtcga tgggttgcgg ttgaatactg
     5041 cccagcaccg ctgcgccacc ggcttcaaac agataattat gcgcggcctg accgacaata
     5101 tactcttcgc cgcgttcgca gtgactgagc agagcgacca ggttagcctg ggtgccggtc
     5161 ggcagaaaaa tggcggcttc tttaccggaa agctctgcgg cgtagtcctg cagagcatta
     5221 acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg cggccatcat tgcttcgagc
     5281 atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat caatcatggc atgtccttat
     5341 tatgacggga aatgccaccc tttttacctt agccagttcg ttttcgccag ttcgatcact
     5401 tcatcgccgc gtccgctgat gattgcgcgc aacatataca agctaaatcc tttggcctgt
     5461 tcgagtttga tctgcggtgg aatggctaac tcttctttgg cgaccaccac atccaccagc
     5521 accggaccgt cgatggagaa ggcgcgttgc agggcttcat caacttcaga cgctttttct
     5581 acacggatac ccgtaatgcc gcacgcttcg gcaatgcggg caaagtttgt gtcgtgtagt
     5641 tcggtgccgt cagtcaaata gccaccagct ttcatctcca tcgccacaaa gcccagcacg
     5701 ctgttgttaa agacgacaat tttcactggc agtttcatct gcactactga gaggaaatcg
     5761 cccatcaaca tgctaaaacc gccatcgccg cacatggcga ccacctgacg ttctggctct
     5821 gtcgcctgcg cacccagcgc ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa
     5881 cctaacaggc gacgcttgcc gttcattttt agataacgtg ccgcccacac cgttggcgta
     5941 ccaacgtcac aggtgaaaat agcgtcatcg gcggcaaaat gactaatttg ctgcgccaga
     6001 tattgcgggt gaatggcttt ctcgctcggt ttagctaaat cgtccagccc tttgcgggcg
     6061 tcgcggtaat cttccagcgc tttatccaga aacttgcgat cggctttttc ttccaccaat
     6121 ggaagcaatg cacgcagagt cgacttgata tcgccgacca gtgccatatc caccttgctg
     6181 tgagcgccga tgctggctgg gttgatatca atctgaatga ttttggcatc ggtcgggtag
     6241 aaggcgcggt agggaaattg cgtgccgagt agcactaacg tgtcggcgtt catcatggta
     6301 tggaaacctg acgagaagcc gattaacccg gtcattccaa catcatacgg attatcgtat
     6361 tcgacatgtt ctttaccgcg cagggcatga acaataggcg ctttaatttt cccggcaaac
     6421 tcaactaact ctttatgcgc ccccgcgcag ccgctgccac acatcagggc gatattgctg
     6481 gaataacgca gcagttgcgc cagtttgcgt aactcttctt cttccggcgt cacgactggt
     6541 tgtggcgcat gataccagtg cgtggttgcc ccttctggcg caggttttaa cgccacgtcg
     6601 cctggtaaca cgacaaccga aacgccacgg ttaagcaccg ctttgcgcat ggcaatcgcc
     6661 agtacttgtg ggatctgctc cgggctggaa accagctcgc aatagtgact acattcgcgg
     6721 aatagctctt gtgggtgggt ttcctggaaa tagccgctgc caatttcgct ggagggaata
     6781 tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc aatcgaacag gccgttgatt
     6841 aagtgcaggt tgccggggcc gcacgatccg gcgcagaccg ccagttctcc gctaagttgt
     6901 gcttcagcgc cagcggcaaa ggccgccact tcttcgtggc gggtggacat ccactcgatg
     6961 gtgcccatgc gattaagact gtcactaaga ccgttcagag agtcgcctgt gactccccag
     7021 atgcgtttca cccctgccga ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc
     7081 atggttctcc atctcctgaa tgtgataacg gtaacaagtt tagttcatct gacggagggg
     7141 gaagggatgg gagagaaagg aggcactaac ggttaaatag cccgatgaaa ggaatatcat
     7201 cgggcataag gcgattatgc gagaaccaaa tccccctgcg gatggcagga gcaggccagt
     7261 acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg tactgctcac cgtatattca
     7321 ccggaaacca cttttgtctt acagcagccg caaacacccg cacggcaggc agcgacaacc
     7381 ggaacgttat tgctttccag cgcctccagt agcgtggtgc caaccggggc gtaaaattct
     7441 cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg ccgcttctgc tactggagtg
     7501 aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa
     7561 tccatatacg gagccgggcc gcaggtcatc acggtacgtg aagctaagtc aggtacacct
     7621 gccagcagtt cgcgagtgag acgaccagcg ataaagcctt cggtaacgtt attttctgcc
     7681 accagcgtta ccggatagtt acgccactca tcggcgaaaa taacatcctg cggcgtacgc
     7741 acgttgtaga tcacccgcac atcggcctgt ggacggttct tcgcaagcca gcgacgcatc
     7801 gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca ggaatttatc ttctgcttta
     7861 tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga gataatcacc gcgttttaca
     7921 tcgcgcgtca gccactggga gccgacaccg tcatcaatcc gccgcacggt cagggtgata
     7981 tattcactca cgcctggcgt ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt
     8041 cgcacgctga ccagtgcata ttgcccggcg cgatatgggt agtaatcgtg gcaaatcagg
     8101 gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg
     8161 cattgattcg ttggcatcgt catcgacaaa ctccttacgc gctcaacagt tgcttcatgt
     8221 cttcttcaac agtggtgata gaacgcaggc cgaatttctc gttcagcacc gccagcaggt
     8281 ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat atttttcacg cccagagaaa
     8341 gcagcgtcag cagaatgacg atcgctttct gttcaaacca ggagagcacc agcgacagcg
     8401 gcagatcgtt cacaccacag cccagtttct ctgccagagt gacagccaga ataatcgctg
     8461 agtaagcatc gttacattga cctgcatcta ccagacgcgg cagaccttcg atatcgccaa
     8521 actcaagttt gttaaagcga tatttaccac aggcgagggt caggatcagg cagtcatccg
     8581 gcacgctggt ggcgaaatcg gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac
     8641 caagcaggaa gatatgacgc agtttttcac ggctcaccag atcaatcagc gtatcagcag
     8701 cgccaagcag cgtctggcga ccaaaaccca cggtgataag gtgcggaatt tcgctgtacg
     8761 ggaagcctgc catctgttgc gcctgggtga taaccgcaga gaaatcatca ccgtccagat
     8821 gacgcacgcc aggccagcca acaatgctgc gggtccagat acgatcgtca taagcgccta
     8881 cggttgggtc gatgatgcag ttcgaggtca tcacgatggg gccagggaaa cgagcgaact
     8941 ccacttgctg attctgccag ccgctgccgt agttaccgac cagatgcttg aatttacgca
     9001 gctccgggta gccatgcgca ggcagcattt cgccgtgggt gtagacatta acgcccgtgc
     9061 cttcggtctg ttccagcagg ttgtagagat ctttgagatc gtgaccggaa atcagaatgc
     9121 atttacccgc cgtcgctttg acgttgacct gggttggcgt cgggtgaccg tatttaccgg
     9181 tttcgcctgc atccagaatg ctcatcactt tgaagttcat ctggccgatt tccattgaac
     9241 actcaagaag cgcgttcata tcggcaggcc aggtccccag ccacgccatg attttatggt
     9301 actgggcata aatatcgttg tcgtattgac cgagaacatg cgcgtgttcc atataggccg
     9361 ccgcaccttt caggccatac aggcacagca gacgcaggcc gagaatgttt tcgccaatcg
     9421 ccgctttatc tttgttaggg gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat
     9481 cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg cgcgttggca tctacagcca
     9541 ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac
     9601 gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt
     9661 caacatcgtg gttgatgatg ccgtattcac acgcttttac cgcccaggca gaaagccctt
     9721 gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc cgccgtttta ccacacatcc
     9781 cctgcgcgta tgagcagccg tttcctgccg gagtacggat agtttgttca cattgcacac
     9841 aaaacatgat cacacctttt aaagttatat ttaatataca tgtttaaggt taagacgctt
     9901 aacgcgggga taaaagggat ttttcatgca actttaaggg agattgattt agcgcaattt
     9961 tggcggcagg gatctaccgc cagagaggta ttacgcagag aaaaaggcga tgaggatcgg
    10021 cactaacagg ctaagaataa aaccgtgaac aattgccgcc gggaccatat ccagcccgcc
    10081 agtacgttga agaacgggca gggtgaaatc cattgatgtg gcaccgcata agcccagtgc
    10141 agtagagcgg ctgcggcgaa tcagcccagg gatcaacata atagcaatca gttcacgggc
    10201 cagatcatta aaaaacgccg cgctcccgat taccggacca aaagattcgg tcaataaaat
    10261 accggaaaga gaataccagc cgaaaccgga ggccattgcc agcgcggtat tgatggggag
    10321 atcaagaata aaggcgttaa ttaaaccacc aattaatgaa ctgacaacca ccaccacggc
    10381 gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat tattgcgcaa
    10441 ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg
    10501 ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca atcactacta cgccgcacag
    10561 ttttagcgac tccagcgcca tcgcaatacg cgacgggagt ttttcttgct gatggtggtt
    10621 gcgccacggc aggcctcgct ccagccacat cagggcggca atattacaca gtaaaataac
    10681 ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga ggttatcgag
    10741 aaacgccaga ctgataccca taaaaaagag aataaggtaa accatccagc ttaatagctg
    10801 attaataact tttaacgcag cttgttggcg aagcggaatg aggtaaccta caatcagggg
    10861 aaccagaatg attaacagcc cagaaaacat gaaaacccag tccttgcaaa gatgaagtcg
    10921 aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa
    10981 atgttatgca taaggagcag tagagtattc gttttcattt aaagatattc ttgcgcttta
    11041 attacaaact gcaccgatgt tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg
    11101 ggcagcccac atggatgtgg gctgagggcg cgttttacag ggatgttacc tcgcgcccga
    11161 cccggtagcc gtaagggata agtcgagggc accgcgcagc ggcgattttg ttcgccagag
    11221 cccggggggg cagggggcgg cggcgattgg ccgccccctg cgcgctcctt gcgccagtgg
    11281 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
    11341 atcttaaaaa aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc
    11401 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
    11461 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
    11521 gggttaacag aagtgttagt caccggaata ctaattaagt gaatcagggt taaggccaga
    11581 ccaatagcga tcggcgcaaa acctgccggc gcgaatttgt cggttgcgcc gtggatcacc
    11641 aacaggaaac ctgcactcaa taccagttca actaccagcg
//
LOCUS       DAZSBF010000123        11620 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZSBF010000123 DAZSBF010000000
VERSION     DAZSBF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.124"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            228..899
                     /locus_tag="ACHXWD_002661"
     CDS             228..899
                     /locus_tag="ACHXWD_002661"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR7621544.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCNQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            909..2105
                     /locus_tag="ACHXWD_002662"
                     /pseudo
     CDS             909..2105
                     /locus_tag="ACHXWD_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2105..2665
                     /locus_tag="ACHXWD_002663"
     CDS             2105..2665
                     /locus_tag="ACHXWD_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR7621545.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2667..3440
                     /locus_tag="ACHXWD_002664"
     CDS             2667..3440
                     /locus_tag="ACHXWD_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR7621546.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3628..3903
                     /gene="frmR"
                     /locus_tag="ACHXWD_002665"
     CDS             3628..3903
                     /gene="frmR"
                     /locus_tag="ACHXWD_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR7621547.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3938..5047
                     /gene="frmA"
                     /locus_tag="ACHXWD_002666"
     CDS             3938..5047
                     /gene="frmA"
                     /locus_tag="ACHXWD_002666"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR7621548.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5140..5973
                     /gene="fghA"
                     /locus_tag="ACHXWD_002667"
     CDS             5140..5973
                     /gene="fghA"
                     /locus_tag="ACHXWD_002667"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR7621549.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6199..6738)
                     /locus_tag="ACHXWD_002668"
     CDS             complement(6199..6738)
                     /locus_tag="ACHXWD_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR7621550.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6840..8051)
                     /gene="mhpT"
                     /locus_tag="ACHXWD_002669"
     CDS             complement(6840..8051)
                     /gene="mhpT"
                     /locus_tag="ACHXWD_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR7621551.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8338..9351)
                     /gene="mhpE"
                     /locus_tag="ACHXWD_002670"
     CDS             complement(8338..9351)
                     /gene="mhpE"
                     /locus_tag="ACHXWD_002670"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR7621552.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9348..10298)
                     /gene="mhpF"
                     /locus_tag="ACHXWD_002671"
     CDS             complement(9348..10298)
                     /gene="mhpF"
                     /locus_tag="ACHXWD_002671"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR7621553.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10295..11104)
                     /gene="mhpD"
                     /locus_tag="ACHXWD_002672"
     CDS             complement(10295..11104)
                     /gene="mhpD"
                     /locus_tag="ACHXWD_002672"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR7621554.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11114..>11620)
                     /gene="mhpC"
                     /locus_tag="ACHXWD_002673"
     CDS             complement(11114..>11620)
                     /gene="mhpC"
                     /locus_tag="ACHXWD_002673"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR7621555.1"
                     /translation="VAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQ
                     PTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGP
                     RLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQL
                     VLNFLARP"
BASE COUNT         2814 a         3131 c         2888 g         2787 t
ORIGIN      
        1 aatacaatgt ataaaacaaa ataactatac ataccaaaca tacaaacact taacatatga
       61 attgcataaa aacattaccc agtcgaacct ccgggtctga aagcccttgc ttttcagtta
      121 ttagcaacat ttaacattta taaattaaca tctggctgcg ccagagatta cgtctctgag
      181 tgcatcgttg tgcttgtagt ataataaata aaatggagtg tcattaagtg gataaggttt
      241 tagattcagc cctcctttct tcggcaaata aaagaaaggg tatccttgct attggcgcac
      301 atcctgacga tatagaatta ggctgtggcg catcgcttgc tcgtcttgcg caaaaaggaa
      361 tttatatcgc agcagtggtg atgactaccg gcaactctgg tacagatgga ataatcgatc
      421 gccatgaaga atcgcgcaac gccctaaaga tattagggtg caaccaaact attcatctta
      481 attttgctga cacccgcgct catttacagc tcaatgatat gatttccgcc ctggaagaca
      541 tcattaaaaa tcaaattcct tctgatgttg aaatcatgcg ggtatatacc atgcatgatg
      601 ccgaccgcca tcaggatcat ctcgctgttt atcaagcttc aatggttgcc tgccgcacta
      661 ttccacaaat tctcggctac gaaaccccaa gtacctggct ttcatttatg cctcaggttt
      721 ttgaatccgt taaagaagaa tatttcacag tcaaacttgc tgcattaaaa aaacataaaa
      781 gccaggaacg acgtgattat atgcgtcatg atcgcctgcg tgcagttgca caatttcgcg
      841 ggcaacaggt caatagcgat ctgggtgaag gctttgtaat tcataaaatg attctttgag
      901 caaattgcat gaaaacctgg atatttatct gtatgtccat agcaatgttg ctatggtttt
      961 taagtacgct aagacgtaaa cccagtcaaa agaaaggctg tattgacgcc attatacctg
     1021 cgtataacga aggcccgtgt ctggcgcagt cactggataa tctactgcgt aacccttatt
     1081 tttgccgggt aatttgcgtt aacgacggct ccacggacaa taccgaagcg gtcatggcgg
     1141 aagtcaaacg caaatggggc gaccgctttg ttgccgtcac gtaaaaaaat accggtaaag
     1201 gtggtgcgct gatgaatggc ctcaattatg ccacctgcga ccaggttttt ttaagtgatg
     1261 ccgacaccta tgttccgccc gatcaagacg gaatgggcta tatgctggca gaaattaagc
     1321 gcggtgctga tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg gggctgttac
     1381 cgcacatccg cgcgaccgta aagttgccga tgattgttat gaagcgcacg ctacagcagc
     1441 tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg aatgttccgt actgatgtat
     1501 tgcgtaagtt cggtttctcg gatcgtacta aagtcgaaga ccttgatctc acctggacat
     1561 tagtggcaaa cggctaccgt attcggcagg cgaatcgctg catcgtatac ccacaggaat
     1621 gcaacagtcc gcgtgaggag tggcgtcgct ggcggcgttg gattgtgggc tacgcggtct
     1681 gtatgcgcct gcataaaagg cttttattta gccgcttcgg tatcttcagt atatttccta
     1741 tgctgttggt tgtgatttat ggcgttggga tttatctcac tacctggttt aatgaattca
     1801 tcaccaccgg gccgcatggt gtggtgttgg caatgtttcc gcttatctgg atcggcgtag
     1861 tttgtgttat tggtgctttt agcgcctggt ttcatcgttg ctggttgttg gtgcctttag
     1921 cgccgctttc cgttgtgtat gtattattag cttatgccat ctggattatc tatggactta
     1981 ttgccttttt tactggacgc gaacctcagc gcgacaaacc cacccgctat tccgcactgg
     2041 tggaagcgtc aaccgcttat tcccaacctt ctgtcacagg aactgaaaaa ctatctgaag
     2101 cttaatgtta aagagaaaaa ttattcagat tgcagaccag gtaattattg atgaaactgc
     2161 aggtgaagtt gttatcggcg cgaatacccg tatttgtcat ggtgccgtta ttcagggtcc
     2221 ggtagtgatt ggcgcaaact gcctgatagg taattatgcg tttattcgtc ctggcacaat
     2281 aatcagcaat ggtgtaaaaa ttggttttgc caccgaaatt aaaaatgcgg ttattgaagc
     2341 ggaagcaacg attggtccgc aatgttatat tgccgactcg gtagttgcaa accaggcata
     2401 tttgggcgca caagtacgta ccagtaatca tcgtctggat gaacagcctg tgtctgttcg
     2461 aactccagag ggaattatcg ctaccggatg cgataaatta ggttgttata tcgggcagcg
     2521 ttcacgcctt ggcgtacaag ttattatttt gcctgggcga attatttccc cgaacacgca
     2581 acttggcccg cgcgtgattg tagaacgtaa tttacctacc ggaacttatt cactccgaca
     2641 agaacttatc cgtacaggag attaaaatga taaaacggac gttattagcg gcggccattt
     2701 ttagcgcatt gcccgcttat gccgggttaa cttccattac cgcgggctac gattttaccg
     2761 attattctgg cgatcatggc aaccgtaatt tagcgtatgc tgaactggtg gcgaaagttg
     2821 aaaacgcaac gctgcttttt aatctttccc aggggcgtcg tgattatgaa actgaacatt
     2881 tcaatgccac tcgcggtcag ggtgcggtct ggtataaatg gaataactgg ctgacaaccc
     2941 gaacgggtat tgcctttgcg gataatacgc cggtctttgc ccgccaggat tttcgtcagg
     3001 atattaacct ggccctgttg ccaaaaacgc ttttcacgac cggttatcgc tacactaaat
     3061 attacgatga tgtcgaagtc gatgcctggc aaggcggcgt atcactctat actggcccgg
     3121 tgatcaccag ctaccgctat acccattatg actccagcga tgcaggtggt agttatagca
     3181 atatgatttc cgtgcgtctg aatgacccgc gcggcactgg ttatacgcaa ctatggctaa
     3241 gccgcggaac aggcgcttac acctatgact ggacgccaga aacacgctac ggcagcatga
     3301 agagcgtcag tttgcaacgt attcaaccgc tgactgagca acttaatctc gggctgacgg
     3361 caggtaaagt gtggtacgac accccaaccg atgattataa cggtctgcaa cttgcagccc
     3421 atctgacctg gaaattctga ttgcttctgc cgcccgctat ccggggcggc cttccctgcc
     3481 gattagcccc actccctttc ctcgttgttt tccgaccaca ttcaccggat aaattttatt
     3541 ctccagcgtt atatactata gggaggtatg cattgacata tagcatacct ccctatagta
     3601 tattgcgtgc agatgatgag gtgcgaaatg cccagtactc cggaagagaa gaaaaaggtc
     3661 cttactcgag ttcgtcgtat tcgggggcag attgatgctc tggaacggtc gctggagggt
     3721 gatgccgaat gccgtgccat actccaacag atcgctgccg ttcggggcgc ggctaacggg
     3781 ctgatggcag aagtgcttga aagccatatc cgggaaacgt ttgaccgaaa tgactgctac
     3841 agccgcgaag tcagccaatc cgttgacgac actattgaac tggttcgagc ctatcttaaa
     3901 tagctgaatc tattaccata ttgaggaaga gcgagagatg aaatcacgtg ctgccgttgc
     3961 atttgctccc ggtaaaccgc tggaaatcgt tgaaattgac gttgcaccac cgaaaaaagg
     4021 tgaagtgctg attaaagtca cccataccgg cgtttgccat accgacgcat ttaccctctc
     4081 cggggatgac ccggaaggtg tattcccggt ggttctcggt cacgaagggg ccggcgttgt
     4141 ggttgaagtc ggtgaaggcg taaccagcgt caaacctggc gaccatgtga tcccgcttta
     4201 caccgcggag tgcggcgagt gtgagttctg tcgttctggc aaaactaacc tctgtgttgc
     4261 ggttcgcgaa acccagggta aaggcttgat gccagacggc accacccgtt tttcttacaa
     4321 cgggcagccg ctttatcact acatggggtg ctctacattc agtgaataca ccgtggtcgc
     4381 ggaagtgtct ctggccaaaa ttaatccaga agcaaaccat gaacacgtct gcctgctggg
     4441 ctgtggcgtg accaccggta ttggcgcggt gcacaacaca gctaaagtcc agccaggtga
     4501 ttctgttgcc gtgtttggtc ttggcgcgat tggtctggca gtggttcagg gcgcgcgtca
     4561 ggcgaaagcg ggacggatta tcgctatcga taccaacccg aagaaattcg atctggcgcg
     4621 tcgcttcggt gctaccgact gcattaaccc gaatgactac gacaaaccga ttaaagatgt
     4681 cctgctggat atcaacaaat ggggtatcga ccataccttt gaatgcatcg gtaacgtcaa
     4741 cgtgatgcgt gcggcgctgg aaagtgcgca ccgcggctgg ggtcagtcgg tgatcatcgg
     4801 ggtggcaggt tctggtcagg aaatctccac ccgtccattc cagttggtca ctggtcgcgt
     4861 atggaaaggt tccgcgtttg gcggcgtgaa aggtcgttcc cagttaccgg gtatggttga
     4921 agatgcgatg aaaggtgata tcgatctgga accgtttgtc acgcatacca tgagccttga
     4981 tgaaattaat gacgccttcg acctgatgca tgaaggcaaa tccattcgaa ccgtaattcg
     5041 ttactgattt cccgcaggta taccccgtcc acttcagacg gggttcttaa tactctcccc
     5101 gggcagccgt ccgggggatt aaccgtgaga taatgactga tggaactcat tgaaaaacat
     5161 gccagctttg gcggctggca aaatgtgtat cggcattatt cccaatcact gaaatgtgaa
     5221 atgaatgtcg gcgtctatct cccaccaaaa gccgcgaatg aaaaattgcc ggtgctgtac
     5281 tggctttcag gcctgacctg caacgagcag aatttcatta ctaaatcggg gatgcagcgt
     5341 tacgcggctg agcacaacat tattgttgtt gcgccggaca ccagtccgcg aggcagtcat
     5401 gtcgcagatg ccgaccgtta cgatctcggg caaggtgccg ggttttacct gaacgcaacg
     5461 caagcgccgt ggaatgaaca ttacaaaatg tatgactata tccgcaacga gctgccggat
     5521 ttagtgatgc aacattttcc ggcaacgacc agaaagtcta tctctggtca ttctatgggc
     5581 gggctgggcg cgctggtgct ggcgttacgt aacccagatg aatatgtcag cgtctcggcg
     5641 ttttcgccca ttgtctcccc atcgcaagta ccgtggggac agcaagcctt tgccgcatat
     5701 cttggtgaaa ataaagatgc ctggttggat tacgatccgg tgagtcttat ttcacaaggt
     5761 caacgcgttg cggaaatcat ggttgatcag gggttgagtg atgattttta cgcggaacag
     5821 ctgcggactc caaatcttga aaagatctgc caggagatga atatcaagac gttaatccgt
     5881 tatcacgagg gttacgacca cagctattat tttgtctcca gttttattgg cgagcatatt
     5941 gcctaccacg ccaataaact gaatatgcgt tgataatagt gcacgactgc cggatgcggc
     6001 gtgaacgcct tatccggcct acacttcgcc cgtaaaccgt aggcctgata agacgcgcaa
     6061 agcgtcgcat caggcatggc gcacgactgc cggatgcggc gtgaacgcct tatccggtct
     6121 acacttcgcc cataaaccgt aggcctgata agacgcgcat agcgtcgcat caggcatctg
     6181 cgcacgactt atctatgttt accacatcaa atcatcaggc actttgaagt cggcatacgg
     6241 atcgtcttca tcctgctctt cggcactgag cgcgctgtgc aacacaatac tgctggcatc
     6301 acgctgagca attttatcgg caacactggc gggaataatg gtgtattcac cttcgctatt
     6361 gttatcgacc aacaagcggg caatcgccag acgaccatta atcaactgcg cctgtgtgag
     6421 cttatcgaca aaaatcttct taatcagatt gccgtcagtg aagttaaaac caatatcgcc
     6481 attggcaatg gtgattcggt tcatttcaat gagctgcttc acctgggctt tatattcttt
     6541 cgccaacgcc gcttgttttt gctgttcgct aagctgttta tcacgctcaa gctgtgcctt
     6601 tttattttct tctaccgcag cccgagcttc acgcgcctga acgcgcgatt ttttcgccgt
     6661 tctctcgacc ttcgccgctt ttttgctggt gactaatcct gctttgagca actgctcttg
     6721 taaggtaagt tttgccatct ttgtttccaa acccgctgaa taatttttag cgagtatacc
     6781 tgtaaacgcc gtgggtgtat tgggggcaag tccctttcgc ccctgcttaa taaggcgcgt
     6841 caggcatcgg cacacggctg cattcttgat ctccgactca tcaaaataaa caccgccaac
     6901 ccagcaacaa gaatacccgg tgcagaagcg gccattacgc cgaccgtgcc agtgcctaat
     6961 gccagcattt tcccggccag taacggaccg ctcatagccc ccagacgccc taccgccacg
     7021 gctgttccca cacctgttgc gcggatctgc gaactgtaaa acaacagtgc cagggcatac
     7081 aaaacgcttt gcccacctgt cgcaaacaac cccgcgacaa atcccgccag caacataccg
     7141 ttaaatgacg acaccgttcc aagcgccagc agcgaagcta acatgccgct ataaatcagt
     7201 agcgacatgg ttactggacg cagcttatcc atcaatgcgc ccaacattaa cgtcccgctt
     7261 gccgccccca tttgcagggc aaacatcacc cctgccgcct gcgatggctg gaatccttgc
     7321 tccaccaaaa gtagcggtag ccagttgatc aacatataaa ccaccagcag agtgaagaaa
     7381 taacacaacc acagcagcag cgtcgcggtt gccgtttctg gcgcaaataa ggcacgcagt
     7441 ggtggcgcag cctgtttttc gccagcgaaa accgctgact ccggcagcca gcgcattaat
     7501 agcggcacca gaatcaacgg caccacacca cctacccaaa acaccgtttg ccatgctaag
     7561 tttgcccccg cgaaacccag tgtcgccgcc agcgccgcgc caatgggaac accgcaatac
     7621 atcaggctca ctgccgtccc acgaaaacgt ggacccgcgg cttcagatgt cagggcgata
     7681 agattcggca acgccgcccc cagcccgaca ccggtcatca gccgcgcaaa gaccagtgag
     7741 gggaaatccc aggcaatcgc cgttgccagt gagaacaaac caaacagcgc aactgagcca
     7801 atcaaaatac gcttacgacc ataacggtct gccagcatcc cgccaaccaa cgcgccgggt
     7861 agcaaaccga gtattccggc gctaaatatc cagcccattt gcattttatc gagtgcgaaa
     7921 gcctgggcga tgccacccgc cgcaatgcca gccgcctgaa gatccagccc ttccatcaga
     7981 gcgaccaaaa aacaaagccc gatggtcagc atcaggcggg atgaagatga tgaaggggta
     8041 cgagtcgaca taagatgaac ctcattgcga gtgcaggtga atcgcgactg atacattgcc
     8101 agccgcgtaa tcagaactat ttaatgactg cctacgggcg aaatgtagcg aaatgtaggc
     8161 cggataaggc gttcacgccg catccggcag tcgtgcgcag atgcctgatg cgacgctaac
     8221 gcgtcttatc agacctacaa attctcacgc catccgtagg ccggacaagg cgttcacgcc
     8281 gcatccggct gtcgtgctgg gatgggcata caaaatgcac gttatcggca gtcatcatta
     8341 tttgttgttg cgcagatcca gcgccacgtc gacgatcata tcctcctggc cgccaaccat
     8401 ccggcgtttg cccagctcaa cgagaatatc caccgcactt aagccataac gcgccgccgc
     8461 cgtttcacag tgacgcagga agctcgagta aacaccagcg tatcccagcg ccagcgtttc
     8521 gcgatcgact cgtaccggac gatcctgcaa cggacgcacc aggtcgtcgg cggcatccat
     8581 taacgcatag agatcggtcc catgctgcca gcccagttta tccgcggcgg caataaacac
     8641 ttccagcggt gcgttacctg cgcccgcgcc cattcccgcg aggctggcgt cgattcggtc
     8701 gcagccctct tccaccgccg cgatagagtt cgccacgcca agactcaggt tatggtgagc
     8761 gtgcatgccg gtttgcgttt ctggtttcaa cactgctttc agggcgcgga aacggtcacg
     8821 gatatcgctc atgttcatcg caccgccaga atccaccaca taaatacagg tcgcaccgta
     8881 gccttccatc agctttgcct gcttggcgag attctccggc gtggtcatat ggctcatcat
     8941 cagaaaacca acggtgtcca ttccgagctc gcgggcatac tgaatatgct gggcggaaac
     9001 atcagcttcg gtacagtgcg ttgccacacg aaccacccgc gcgccagcct gccaggcatt
     9061 tttcagatcg tgaatagtgc cgattcctgg cagcaacaac gtcgcgattt tggcgtgcct
     9121 caccacatcc gccgccgctt caatccattc aaggtcgcta tgtgcgccga aaccatagtt
     9181 aaagctggaa ccttgcaaac cgtcgccgtg ggccacttca atcgaatcca cgcgggcatc
     9241 gtccagtgct ttggcaatct ggcgaacgtt ttccagcgaa tactgatgac gaatggcgtg
     9301 cataccgtca cgcaatgtga cgtccgagat ataaagtttt ttaccgttca tgccgcttct
     9361 cctgccttgc gcgccagtga ctgggccatt ttttccgctg tcgccagcgc actggaagtc
     9421 ataatatcga ggttgcccgc ataggcaggc agataatgcg ctgcgccttc gacttccagc
     9481 cagaccgctg ttttcagtcc ggagaattgc cccacgcccg gtaaattgac cggtttatcc
     9541 tgcgggataa cttcaaactg cacgcgctgt ttcaggcgat aacccggtac gtaagcctgc
     9601 accgcctcag ccatttcatt gattgaggct tcgatatcat cttgtgaagc ttcgtcgctc
     9661 aatacataca ccgtgtcacg catcatcaac ggtggctctg ctgggttaag cacaataatc
     9721 gccttccctt ttgccgcgcc gcccaccact tcaatggctc gggaagtggt ttccgtaaat
     9781 tcatcgatat tggcacgcgt gccaggtccg gcagatttac tggcgataga agcgataatt
     9841 tcggcgtaat gaacacgcgc cacgcgtgaa actgccgcca ccattggaat ggtggcctgg
     9901 ccgccgcagg tgaccatgtt gacgttcagt tgatcgacgt tcgcctcgag gttaaccacc
     9961 ggcacgcagt aagggccgat ggcagcaggc gtcaggtcaa ttaagcgaat atccggtttc
    10021 gcttcgcgta aagcggcatc gtttttcaca tgcgcgcccg cgctggtcgc atcaaataca
    10081 atgtcgatat cagcaaattc aggcatgttc atcagcccga tcaccccttc atgggtggtg
    10141 gcgacgccca tacgtctggc acgcgccaga ccgtcggact gaggatcaat gccgaccatc
    10201 accgccatct ccagatgctg accgtgacgc aaaattttaa tcatcagatc ggtgccaatg
    10261 ttgccagaac cgataatggc gactttacgc ttactcatga cagacttcct tttggggctg
    10321 cgcttgaaaa tgtcgcagca actgaaccta tgccttcaat atgggcttca aaacgatcgc
    10381 ccgcattcac cgccaccatc gggcctaatg ccccggtaag aatgatatct ccggcgcgca
    10441 gcggttcacc cagactggcc attttgcgtg ccagccagac ggcggcatta agcggatgtc
    10501 ccaggcattc gctgccgcgc ccgctggaaa cctcttcgtt attacgcgtc atcttcatgg
    10561 cgcagttttt caggtctaac cccgccggac gttgcgccgg accgccgatg acatacaccc
    10621 cgcaggaagc gttatctgcc acggtatcga caaactgaat cgaccagtcg cgaatgcggc
    10681 tccccaccac ttccagcgcc ggaagtaccc attcaatggc gttatacagt tcgtcgaagg
    10741 tgatatcagt tgcgggtaaa tcgcggttca acaccagtgc gatctccgct tcaatgcggg
    10801 gttgcagaac acgggaaaaa ggaatgattt cgttatcgcc ataacacatg tcggcaaata
    10861 acgtcccaaa atccggttga tcaacgccca gttgttgttg cactttcgga tgcgtcaggc
    10921 ccactttacg ccctaccacg cgacgcccct gcacaacgtc atattgcaca tttatgtgct
    10981 gaatggcgta ggccgcttca gcgtgatcga taccaatcag atcgcgcagc ggcgcaatcg
    11041 cttcgccctg ctctgcggcg cggcgtaaat ccgccgccag ttgctcaaga gtatgcttcg
    11101 tcatgaccat tccttaaggg cgtgcgagga aattcagcac caattgattg aaagcgtcgg
    11161 catgttccca ctgcgcccag tgaccacagt cgcggaagat atgcagttca gaaccggcaa
    11221 tgccggacag cagacgcaga cccgcatcca tcggcacaaa gcggtcgttg cgcccccaga
    11281 caatcagggt ttgcgctttg atttccgcca gacgtgggcc aaaatccggg aactgtttcg
    11341 gattagcttc caggctctta acgaagtttt ccaggtgatc gcggcgcgac agcatattat
    11401 tcaggcgcgc ttcaaacagg gcgtcggtca aatcgctggt atcgaaaacg aagatatcca
    11461 tcatcagctt caggttttcg atagtcggct gacgataaag ctgattcagt cgcttaatac
    11521 cttcggttgg catcggcgta aacaaactca tgccgcccgt accgccgccc atcagcacca
    11581 gtttgccgac gcgctccggc cagttaaggg tgaacgccac
//
LOCUS       DAZSBF010000124        11559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZSBF010000124 DAZSBF010000000
VERSION     DAZSBF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.125"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHXWD_002674"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHXWD_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR7621556.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHXWD_002675"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHXWD_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR7621557.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHXWD_002676"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHXWD_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR7621558.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHXWD_002677"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHXWD_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR7621559.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHXWD_002678"
     CDS             7014..7292
                     /locus_tag="ACHXWD_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621560.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHXWD_002679"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHXWD_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR7621561.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHXWD_002680"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHXWD_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR7621562.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHXWD_002681"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHXWD_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR7621563.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHXWD_002682"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHXWD_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR7621564.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="ACHXWD_002683"
     CDS             10284..10931
                     /locus_tag="ACHXWD_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR7621565.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="ACHXWD_002684"
     CDS             complement(10973..11503)
                     /locus_tag="ACHXWD_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR7621566.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT         3198 a         2573 c         2631 g         3157 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggtttt ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaac
//
LOCUS       DAZSBF010000125        11512 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZSBF010000125 DAZSBF010000000
VERSION     DAZSBF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.126"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(212..1483)
                     /gene="efeB"
                     /locus_tag="ACHXWD_002685"
     CDS             complement(212..1483)
                     /gene="efeB"
                     /locus_tag="ACHXWD_002685"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR7621567.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(1489..2616)
                     /gene="efeO"
                     /locus_tag="ACHXWD_002686"
     CDS             complement(1489..2616)
                     /gene="efeO"
                     /locus_tag="ACHXWD_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR7621568.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(2674..3504)
                     /gene="efeU"
                     /locus_tag="ACHXWD_002687"
     CDS             complement(2674..3504)
                     /gene="efeU"
                     /locus_tag="ACHXWD_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR7621569.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(4170..5678)
                     /gene="putP"
                     /locus_tag="ACHXWD_002688"
     CDS             complement(4170..5678)
                     /gene="putP"
                     /locus_tag="ACHXWD_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR7621570.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            6100..10062
                     /gene="putA"
                     /locus_tag="ACHXWD_002689"
     CDS             6100..10062
                     /gene="putA"
                     /locus_tag="ACHXWD_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR7621571.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(10102..10740)
                     /gene="rutR"
                     /locus_tag="ACHXWD_002690"
     CDS             complement(10102..10740)
                     /gene="rutR"
                     /locus_tag="ACHXWD_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR7621572.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            11028..>11512
                     /locus_tag="ACHXWD_002691"
     CDS             11028..>11512
                     /locus_tag="ACHXWD_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR7621573.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         2766 a         3202 c         2939 g         2605 t
ORIGIN      
        1 agtttctact ggaaagatga cagagtgatg acagtgatga aaaaagctgt gtgctttcag
       61 caggatttgc atcaggcatc agtgcacgat cgccggatgc ggcgtgaacg ccttatccag
      121 cctaccaaac ccgcactgat tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc
      181 tgcgcacaaa tgccttatcc gcctaaaaac attaaacccg caataacgcg cttccgagat
      241 aatcgttcgc gtccttcact cccggcagcg aaaaaaaata accgccgccg ataggtttaa
      301 cgtattcctc cagcgcttcg ccattgagcc ttttttgtac tgtcaggaag cctttttcca
      361 gatcgtgttg gtagcagaca aacagcaacc ccatatccag ttgtccggag ttggtgaccc
      421 ccaacgaata actgtagccg cgacgcagca tcaggctgga ctcactctcc gccgtgcggg
      481 gattcgccag ccggatatgg ctgtccagcg caatcacctt cccttccggg tcgctggcgt
      541 aatcaggcac atcatgctca tgctgcattc ccagcggcgc accggtttgc ttatcacggc
      601 caaaaatcgt ctgctgttct ttcagcggcg ttctgtccca aaattccact cgaaactgaa
      661 tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc aggctcctgc tgatctgccg
      721 ttacccacac cactttttgc atcaacttat cattctggct atcgggattg gcagtgccgt
      781 ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgac acgtgatcgg
      841 aaataaaccc ttcccgcttc cagcgcacac tgagcaaatc cggcgtgtgt ttgatgatat
      901 cgcgcagcgc atggataacc gtgtcctggg tgttggcgca aatctgtagc aacacatcac
      961 catgacataa cgccgcatcc agcgagtcgt tggggaaacg cgtcatcttc tgcagctttt
     1021 ttggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc accgataacg
     1081 tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg agtggtggca
     1141 ggcgcggatt tggcgtttct ggtgctgctc cgccctgagt cagaaaagca aaacgctgag
     1201 tcaacaagcg aaacaaccgc tcaagatcgg ctttatcgct ggcaagcaca tcaaacgcca
     1261 ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca ccataaaacg
     1321 gctgtttctc attgcgtgca tccggtgaaa gcgtgcccgg tgcactttgc gttttttgtg
     1381 catgagcgac cggacaactt cccgccagcg ccagcgcacc aatccctttc agtaaacgtc
     1441 ggcgtgacgg ttcattcacg ccgttttcat ctttatactg cataacgctt aatccagtcc
     1501 cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc ctttcagtgc
     1561 attccggtcg gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag tacggtattt
     1621 cgccagaatg gtatcgacct ttttaaagtt ggcatcgact tttgccagca gttccgggtt
     1681 ggctttttgc agttgtggac gcagcaaatc gacaattttc tgcgagcctt caacgttagc
     1741 ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctaa ttttgctggc
     1801 tgccacttcc tcaatcagtc cggctgcgcc gccgaccact tttgaaggtg ggaaagccag
     1861 ttcactgatg cgtttttgca aatcgaccac atctgtataa agctgctcag cgtactgatc
     1921 catccctttg gtggtgttgt cgccaaacaa tgctttttcc agacggtgga aaccagtgaa
     1981 ttttgggtcg gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc tgccatccag
     2041 atcggagaac agttcagcaa tcggttcaat gcgctcatag tgctggcgcg tcggcgcata
     2101 cagtgctttc gctttttcga tatcgcctgc tttaatcgcg tcggtaaagg ctttggtgtc
     2161 ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa ttgcaccacc
     2221 aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt tgacgatcaa
     2281 cttccctttc gggttagtca gcagaccgca ggtcatatcg tattcgccag gctgtaaatt
     2341 cgccgtcatt ttctggctaa aaccaggggc gatattttcc cgctcttcca ccaccatcac
     2401 gcctttgagg atctcccact ccagcgcctt ctggctatgg ttctgaataa tgaactgtgt
     2461 tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatccgtca cggtcacttt
     2521 gacctgcggc acatcagcgg cgttagccat aaaagcagaa gaaaacagcg cagccacgct
     2581 caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc tttaaagtat
     2641 gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg ctgtcgcccc
     2701 tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg agataaataa accagacggc
     2761 gacttcgctg acgctcggtg cttcctgata gccaaaaatc ccttccatca gcgtgccaaa
     2821 cagcgagtga gttgagagca ctgcgctcat atcgaaggcg atttcctgaa agtggttcca
     2881 caatccggct tcatgaaatg cgcgaatggc accagcagcc agccctgcgg cgacgaagag
     2941 aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac cgccccagta
     3001 gagcaggaag cctagcacca cggcagtagc aagaccgagc attgcaccca gcggcggcca
     3061 gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca gcccttccct
     3121 tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc cacgctgcaa
     3181 tgcgctatcg actgcctgct ccagttgtac tttgacgttg cgcgacactt tgcgcatcca
     3241 gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt caaacagttc
     3301 ctgttctttt tgcggaaatt cgccggtggt ttcgttaatg aagataccca agcccaggca
     3361 caacgcagcg gcaagcaaca cgccaatcca catcacgcca atccatcggc ctcgctgggt
     3421 acgcttaaga tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc cttcgcgcaa
     3481 cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg agtcaatcca
     3541 tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggcaaagaa aaatacaagc
     3601 ggaatgttta taaaatgatg ttggaatgta aacaaagaga ggtaatacca ggggatataa
     3661 acgattattc caggtacaaa gatgggattg tttacaacga gccatgtgtt cttgccagat
     3721 gccgcgtaaa cgccttatcc agccgacaaa actgtccaga ctcaatgcat cacagcttcc
     3781 atgtaaaact gataagcggg tgtatcaagc aatttcgtcc atccgcgatt atcaaatcta
     3841 actttttgcc cggaacataa taacagcgcg ggtgttggtg gcgtcaaaat cgctgaaacg
     3901 ttccccgaag gccggggcag cccacatgga tgtgggctga gggcgcgtct tacaggacgt
     3961 tacctcgcgc ccgacccggt agccgaaggg gataagctga aggtaccgcg cagcggcgat
     4021 tttgttcgcc agagcccggg ggtgcagggg gcggcggcga ctggccgccc cctgcgcgct
     4081 ccctgcacca ttcaatttat atggcgaggc acattaaggg agcgcaaccc gctgctgaag
     4141 ccaaacaaaa ccgccgcagg ctaagtccct tagttttcct gcaaccgtga cggcggagcc
     4201 gaatgatagt gcgcatcggc ctcggcaaag cgtttttgca tcgccgctga cggcgcttta
     4261 cccagcaaac taaacactac aatcccgata ctgccgaaga tgaagcccgg aataatttcg
     4321 tacagtccca gccagccgaa ctgcttccag acgataactg tcagcgcacc gatgatcatc
     4381 cccgccagcg caccgttgcg cgtcatgcgt gaccacatca ccgagaacag caccaccgga
     4441 ccaaacgccg cgccaaagcc tgcccacgcg tagctcacta agcccagcac gcggttttcc
     4501 gggtttgccg ccagcgcaat cgccaccagc gccaccacca gcaccatcac acgtcctacc
     4561 cacaccagct ctttctggct ggcctgttta cgcagaaacg ctttgtataa atcttcggta
     4621 atcgcactgg agcacaccag cagctggcaa cttaaggttg acattaccgc cgccagaatc
     4681 gccgacagca gaatcccggc aatccacggg ttaaacagaa tttgcgccag ttcgataaac
     4741 acgcgttcag cgttctggtt taccgcacct gctaccgccg gatgctcgtt aaagtaagcg
     4801 ataccaaaga agccaacagc caccgcccct gccaggcaaa ggatcatcca ggtcatactg
     4861 atacgacgcg catggacaat gctgtggtga gaatctgccg ccataaaacg cgccaggatg
     4921 tgcggctgcc cgaagtaacc cagcccccaa cccatcaatg agataatggc aacaaagttc
     4981 agccctttga gcatatccac gttttcgatg ctcttttgtt tgatcacttc cagcgagtca
     5041 ccaaagccac cgacactgat aatgacgata accggcgtca ggatcagggc aaaaatcatc
     5101 aggctggcct gtacagtgtc agtccagctc accgcgagga aaccgccaat aaaggtgtaa
     5161 aggatcgtcg acgcagctcc cgccaacaga gccgtttcgt aactcatgcc aaaggtactt
     5221 tcaaacagac gcgcgcctgc cacaatgccc gaagcgcaat aaatggtgaa gaacagcaaa
     5281 ataaccagcg cggagataat gcgcaaaatg cggcttttat cttcaaaacg cccggtgaaa
     5341 taatccggca atgttaaggc gttattattg tattcggtat gcacacgcaa ccgcccggcc
     5401 accagcttcc agttgatcca cgcgcctaat gtcaggccaa tggcgatcca gctttcggaa
     5461 atcccggaaa gaaaaaccgc gccaggcaac cccattaaca gccagccgct catatccgac
     5521 gcacccgccg ataatgccgt cacgaatggc ccaagactac gaccgcccag aatatagtcg
     5581 tcaaagtttt tcgttgatcg ccaggcgata aacccaatca atatcatgcc aaagatatag
     5641 acacaaaatg tcaccaacat cggtgtgcta atagccatct aaagtctcca aaaaattatt
     5701 atcggcaatg tcgaaacttg ccgttatatc tgccaccgga acggggtaac agagtttatg
     5761 ttttaccagg gcgaccgtat cctgccggaa gcgctggtta ttcacaatcg atttaacaca
     5821 ccatttacat taaattttag tgctcagcga cactattttt catcaggttg cactctctca
     5881 cattttttgc ggttgcacct ttcaaaaagg ttaactgccg cagagaaaaa gtctgagtta
     5941 ttttttaatc actgtcatat cgatttcttt tattaacatt tcattcattt ttaagcttgc
     6001 tacgcaagtc acatttaaca tggttgcaca aagttgcaac atcatggata tttcacgata
     6061 acgttaagtt gcacctttca gaacaacagg agtaatggca tgggaaccac caccatgggg
     6121 gttaagctgg acgacgcgac gcgtgagcgt attaagtctg ccgcgacacg tatcgatcgc
     6181 acaccacact ggttaattaa gcaggcgatt ttttcttatc tcgaacaact ggaaaacagc
     6241 gatactctgc cggagctacc tgcgctgctt tctggcgcgg ccaatgagag cgatgaagca
     6301 ccgactccgg cagaggaacc acaccagcca ttcctcgact ttgccgagca aatattgccc
     6361 cagtcggttt cccgcgccgc gatcaccgcg gcctatcgcc gcccggaaac cgaagcggtt
     6421 tctatgctgc tggaacaagc ccgcctgccg cagccagttg ctgaacaggc gcacaaactg
     6481 gcgtatcaac tggccgataa actgcgtaat caaaaaaatg ccagtggtcg tgcaggaatg
     6541 gtccaggggt tattgcagga gttttcgctg tcatcgcagg aaggcgtggc gctgatgtgt
     6601 ctggcagaag cgttgttgcg tattcccgac aaagccaccc gcgacgcgtt aattcgcgac
     6661 aaaatcagca acggtaactg gcagtcacac attggtcgta gcccgtcact gtttgttaat
     6721 gccgctacct gggggctgct gtttactggc aaactggttt ccacccataa cgaagccagc
     6781 ctctcccgct cgctgaaccg cattatcggt aaaagcggtg aaccgctgat ccgcaaaggt
     6841 gtggatatgg cgatgcgcct gatgggcgag cagttcgtca cgggtgaaac catcgcggaa
     6901 gcgttagcca atgcccgcaa gctggaagag aaaggtttcc gttactctta cgatatgctg
     6961 ggcgaagctg cactgactgc cgcagacgca caggcgtata tggtttccta tcagcaggcg
     7021 attcacgcca ttggtaaagc gtctaacggt cgtggcatct atgaaggtcc gggcatttca
     7081 atcaaactgt cggcgctgca tccgcgctac agccgcgccc agtatgaccg ggtaatggaa
     7141 gagctttacc cgcgtctgaa atcactcacc ctgctggcgc gtcagtatga tattggtatt
     7201 aacatcgatg ccgaagaggc cgatcgtctg gagatctccc tcgatctgct ggaaaatctc
     7261 tgtttcgagc cggaactggc aggctggaac ggcatcggtt ttgttattca ggcttatcaa
     7321 aaacgctgcc cgttggtgat cgattacctg gttgatctcg ccacccgcag ccgtcgccgt
     7381 ctgatgattc gcctggtgaa aggcgcgtac tgggatagtg aaatcaagcg tgcgcagatg
     7441 gacggccttg aaggctatcc ggtttatacc cgcaaggtgt ataccgacgt ttcttatctc
     7501 gcctgtgcga aaaagctgct ggcggtgccg aatctaatct acccgcagtt cgcgacgcac
     7561 aacgcccata cgctggcagc gatttatcaa ctggcaggtc agaactacta tccgggtcag
     7621 tacgagttcc agtgcctgca tggtatgggc gagccactgt acgagcaggt caccgggaaa
     7681 gttgccgacg gtaaacttaa ccgaccgtgt cgtatttatg ccccggttgg cacacatgaa
     7741 acgctgctgg cgtatctggt gcgtcgcctg ctggaaaacg gtgctaacac ctcgtttgtt
     7801 aaccgtattg ccgacacctc tttgccactg gatgaactgg tcgccgatcc ggtcactgct
     7861 gtagaaaaac tggcgcaaca ggaagggcaa actggattac cgcatccgaa aattcccctg
     7921 ccgcgcgatc tttacggtca cgggcgcgac aactcggcag ggctggatct cgctaacgaa
     7981 caccgtctgg cctcgctctc ctctgccctg ctcaatagtg cactgcaaaa atggcaggcc
     8041 ttgccaatgc tggaacaacc ggtagcggca ggtgagatgt cgcccgttat taaccctgcg
     8101 gaaccgaaag atattgtggg ctttgtgcgt gaagccacgc cacgtgaagt agaacaggcg
     8161 ctggaaagtg cggttaataa cgcgccaatc tggtttgcca cgcctccggc tgaacgcgcg
     8221 gcgattttgc atcgcgctgc cgtgctaatg gaaagccaga tgcagcaact gattggtatt
     8281 ctggtgcgtg aagccggaaa aaccttcagt aacgccattg ccgaagtgcg cgaagcggtc
     8341 gattttctcc actactacgc cggacaggtg cgggatgatt tcgctaacga aacccaccgt
     8401 ccattagggc ctgtggtgtg tatcagtccg tggaacttcc cgctggctat tttcaccggg
     8461 cagatcgctg ccgcactggc ggcaggtaac agcgtgctgg caaaaccggc agaacaaacg
     8521 ccgctgattg ccgcgcaagg gatcgccatt ttgctggaag cgggtgtacc ccctggcgtg
     8581 gtgcaattgc tgccaggtca gggggaaacc gtgggcgcgc aactgacggg tgatgatcgc
     8641 gtgcgcgggg tgatgtttac cggttcaacc gaagtcgcta cgttactgca gcgcaatatc
     8701 gccagccgcc tggacgctca gggtcgccct attccgctca tcgctgaaac cggcggcatg
     8761 aacgcgatga ttgtcgattc ttcagcactg accgaacaag tcgtcgtgga tgtgctggcc
     8821 tcggcgttcg acagtgcggg tcagcgttgt tcggcactgc gcgtgctgtg cctgcaagat
     8881 gagattgccg accacacgtt gaaaatgctg cgcggtgcaa tggccgaatg ccggatgggt
     8941 aatccgggtc gcctgaccac cgatatcggt ccggtgattg atagcgaagc gaaagccaat
     9001 attgagcgcc atattcagac catgcgtagc aaaggccgtc cggtgttcca ggcggtgcgg
     9061 gaaaacagcg aagatgcccg tgaatggcaa agcggcacct ttgtcgcccc gacgctgatc
     9121 gaactggatg actttgccga attgcacaaa gaggtctttg gtccggtgct gcatgtggtg
     9181 cgttacaacc gtaaccagct accagagctg atcgagcaga ttaacgcttc cggctatggt
     9241 ctgacgcttg gtgtccatac gcgtattgat gaaaccatcg cccaggtcac tggctcggcc
     9301 catgttggta acctgtatgt taaccgtaat atggtgggcg cagtggttgg tgtgcagccg
     9361 ttcggcggcg aagggttgtc cggtaccggg ccgaaagcag gcggtccgct ctatctctac
     9421 cgtctgctgg cgaatcgccc ggaaagtgcg ctggcagtga cgctcgcgcg tcaggatgca
     9481 gagtatccgg tcgatgcaca gttgaaagcc gcattgactc agccgctaaa tgcactgcgg
     9541 gaatgggcag caaatcgtcc agaattgcag gcgttatgta cgcaatatgg cgagctggcg
     9601 caggcaggaa cacaacgatt gctgccgggg ccgacgggtg aacgcaacac ctggacgctg
     9661 ctgccgcgtg agcgcgtgtt gtgtattgcc gatgatgagc aggatgcgct gactcagctc
     9721 gccgccgtgc tggcggtggg cagccaggta ctgtggccgg atgacgcgct gcatcgtcag
     9781 ctagtaaagg cattgccatc ggcagtcagc gaacgtattc aactggcgaa agcggaaaat
     9841 ataaccgctc aaccctttga tgcggtgatc ttccacggtg attcggatca gcttcgcgca
     9901 ttgtgtgaag ccgttgctgc gcgggatggc gcaattgttt cggtgcaggg ttttgcccgt
     9961 ggcgaaagca atatccttct ggaacggctg tatatcgaac gttcgctgag tgtgaatacc
    10021 gctgccgctg gcggtaacgc cagtttaatg acgatcggtt aaacaggtag ccggaggatg
    10081 ttacaacctc ctccggcatc tttaacgtgg tcgaatcccc tcaataataa tccgctgcac
    10141 gttttcaacc gtttgattga aaaatatctc atcgcgcaac gtcgcgcctg tcaccgcctc
    10201 cacctgaggg gcgaaatcgg cgtaatgttg agtggaagcc caaatcatga aaatcaaatg
    10261 ctgcggatca atcggcgcga gtttgccgct tttgacccaa ccggcaatca gcgcagattt
    10321 ctcatcaatt aatgccttca aatcgcccgt cagttcatcc attaacagcg gcgcgcctgc
    10381 cagcatctcc atacagaaca ggcgcgaagc ctgcggataa tcgcgggaga cttccagctt
    10441 cagacggatg tactctttta tcgccgccag cggagcgaaa tcttcacgaa acgcttttaa
    10501 cggtgccagc cagatatcga gaatctgccg cagcacggca atatacagcg cctcttttga
    10561 cggaaagtaa tacagcagat tggtttttga aacacccgcc aactctgcga tctgctccag
    10621 ccttgtgccg tgaaaaccga attgtgaaaa agtgtccagt gctgcgctaa gaatcgcttt
    10681 tttcttcgcg cttactgcgc gcgaacgttt acccgttgtt ttcactgcgc cttgcgtcat
    10741 gcgctctccc ctcttgtaga ttggcaacag gatagcaaaa cccccgcccg gcctcgacct
    10801 tctgcactct catcgcgcac agatgcatgt tttatgtgca actgttttga ccgtttagtc
    10861 cactttttat cagatatttt agataacgta atattaacat attaataaca ttaacttttt
    10921 aaaaactggc atccgctttg caaacaagcc aatacgcggc tgaaaaggat atgcaggatg
    10981 cagcgccccg tctgactttc actttacgag acgaagagag gttgatgatg aaaattggcg
    11041 tattcgtacc tattggcaac aacggctggc tcatttcgac ccacgcgccg cagtacatgc
    11101 cgacctttga actgaataaa gccattgtgc agaaagcgga gcattaccat ttcgatttcg
    11161 ccctgtcgat gatcaaactg cgtggctttg gcggcaaaac tgagttctgg gatcacaacc
    11221 ttgagtcgtt caccttgatg gcgggtctgg cagccgtgac ctcgcgcatt cagatttacg
    11281 ccactgctgc caccttaacg ttacctccgg caatcgtcgc ccgtatggcc gcaaccatcg
    11341 actccatctc tggcgggcgt tttggcgtca acctcgtgac tggctggcaa aagcccgtgt
    11401 atgagcagat gggtatctgg cctggcgatg actatttctc ccgtcgttac gactatctca
    11461 ccgaatatgt tcaggtgctg cgcgacctgt ggggcacggg gaaaagcgat tt
//
LOCUS       DAZSBF010000126        11426 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZSBF010000126 DAZSBF010000000
VERSION     DAZSBF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.127"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHXWD_002692"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHXWD_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR7621574.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHXWD_002693"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHXWD_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR7621575.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHXWD_002694"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHXWD_002694"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR7621576.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHXWD_002695"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHXWD_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR7621577.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHXWD_002696"
     CDS             complement(3928..5214)
                     /locus_tag="ACHXWD_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR7621578.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHXWD_002697"
     CDS             complement(5235..6026)
                     /locus_tag="ACHXWD_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR7621579.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHXWD_002698"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHXWD_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR7621580.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHXWD_002699"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHXWD_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR7621581.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHXWD_002700"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHXWD_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR7621582.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHXWD_002701"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHXWD_002701"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR7621583.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHXWD_002702"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHXWD_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR7621584.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHXWD_002703"
     CDS             complement(9882..10364)
                     /locus_tag="ACHXWD_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR7621585.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11426)
                     /gene="dgcN"
                     /locus_tag="ACHXWD_002704"
     CDS             complement(10380..>11426)
                     /gene="dgcN"
                     /locus_tag="ACHXWD_002704"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR7621586.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2802 a         2746 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagt
//
LOCUS       DAZSBF010000127        11417 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZSBF010000127 DAZSBF010000000
VERSION     DAZSBF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.128"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..456
                     /locus_tag="ACHXWD_002705"
     CDS             <1..456
                     /locus_tag="ACHXWD_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR7621587.1"
                     /translation="GLCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFA
                     DWYQQPVFASLNDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFY
                     YLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            471..1328
                     /gene="menB"
                     /locus_tag="ACHXWD_002706"
     CDS             471..1328
                     /gene="menB"
                     /locus_tag="ACHXWD_002706"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR7621588.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1328..2290
                     /gene="menC"
                     /locus_tag="ACHXWD_002707"
     CDS             1328..2290
                     /gene="menC"
                     /locus_tag="ACHXWD_002707"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR7621589.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAEMADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            2287..3708
                     /gene="menE"
                     /locus_tag="ACHXWD_002708"
     CDS             2287..3708
                     /gene="menE"
                     /locus_tag="ACHXWD_002708"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR7621590.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            3752..4018
                     /gene="pmrD"
                     /locus_tag="ACHXWD_002709"
     CDS             3752..4018
                     /gene="pmrD"
                     /locus_tag="ACHXWD_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR7621591.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(4012..4398)
                     /gene="arnF"
                     /locus_tag="ACHXWD_002710"
     CDS             complement(4012..4398)
                     /gene="arnF"
                     /locus_tag="ACHXWD_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR7621592.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4398..4733)
                     /gene="arnE"
                     /locus_tag="ACHXWD_002711"
     CDS             complement(4398..4733)
                     /gene="arnE"
                     /locus_tag="ACHXWD_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR7621593.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4730..6382)
                     /gene="arnT"
                     /locus_tag="ACHXWD_002712"
     CDS             complement(4730..6382)
                     /gene="arnT"
                     /locus_tag="ACHXWD_002712"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR7621594.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6382..7272)
                     /gene="arnD"
                     /locus_tag="ACHXWD_002713"
     CDS             complement(6382..7272)
                     /gene="arnD"
                     /locus_tag="ACHXWD_002713"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR7621595.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7269..9251)
                     /gene="arnA"
                     /locus_tag="ACHXWD_002714"
     CDS             complement(7269..9251)
                     /gene="arnA"
                     /locus_tag="ACHXWD_002714"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR7621596.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9251..10219)
                     /gene="arnC"
                     /locus_tag="ACHXWD_002715"
     CDS             complement(9251..10219)
                     /gene="arnC"
                     /locus_tag="ACHXWD_002715"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR7621597.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10223..11362)
                     /gene="arnB"
                     /locus_tag="ACHXWD_002716"
     CDS             complement(10223..11362)
                     /gene="arnB"
                     /locus_tag="ACHXWD_002716"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR7621598.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2778 a         2952 c         3067 g         2620 t
ORIGIN      
        1 gggctttgtg gggttgttgt cgaaggcggg catcctgggt tgcaaaatgc tgaacaacgc
       61 gcggaacgtc agcgttccga tcgccaatgg gcgcagcgtt ttcgcacaga accgttaacg
      121 gcggtatttg ccgactggta tcaacagcct gtttttgcct cgctcaatga cgatcaacgc
      181 cgggagctgg tggcgctgcg cagtaacaat aatggcgcaa cccttgccgc catgctggag
      241 gcgacttctc tcgccgtcca gcctgattta cgtgctaacc ttagcgcccg cacatttgcg
      301 ttttattatt tatgtggtga acgtgacagc aaattccgcg ccttggcggc ggaactggct
      361 gccgactgcc atgtcattcc tcgcgccgga cataacgcgc atcgggaaaa tcccgctggc
      421 gttatcgcaa gtctggcgca gatcttgcgt ttctgactaa aggacacaat atgatttatc
      481 ctgatgaagc aatgctttac gcaccggttg aatggcacga ctgctccgaa ggttttgagg
      541 acattcgtta tgaaaaatcc accgacggta tcgcaaaaat caccattaat cgtccgcagg
      601 tgcgcaatgc cttccgtcct ctgacggtaa aagagatgat ccaggcactg gcagatgcgc
      661 gttatgacga caacgtcggc gtgatcattc tgactggcgc aggcgataaa gcgttctgct
      721 ccggtggtga ccagaaagtg cgtggtgatt acggcggcta taaagatgat tccggcgtac
      781 atcacctgaa cgtgctggac ttccagcgtc agatccgtac ctgtccgaaa ccggttgtcg
      841 cgatggtggc tggctactcc atcggcggtg gtcacgttct gcacatgatg tgcgacctga
      901 ctatcgcggc agataatgcc atcttcggtc agactggccc gaaagtcggt tccttcgacg
      961 gcggctgggg cgcttcctac atggctcgca tcgtcggaca gaaaaaagcg cgtgaaatct
     1021 ggttcctgtg ccgtcagtac gatgcaaaac aggcgctgga tatgggcctt gtcaacaccg
     1081 tggtaccgct ggcggatctg gaaaaagaaa ccgtccgttg gtgccgcgaa atgctgcaaa
     1141 acagcccgat ggcgctgcgc tgcctgaaag ctgcgctgaa cgccgactgt gacggtcagg
     1201 cgggtctgca ggagctggcg ggcaacgcca ccatgctgtt ctacatgacg gaagaaggtc
     1261 aggaaggtcg caacgctttc aaccagaaac gtcagcctga tttcagcaaa tttaaacgga
     1321 atccgtaatg cgtagcgcgc aggtataccg ctggcagatc cccatggacg cggggatggt
     1381 tctgcgcgac aggcggttaa aaacccgcga cgggctgtat gtttgcctgc gtgaaggcga
     1441 gcgcgaaggg tggggggaga tctccccact gccgggcttc agtcaggaaa cctgggaaga
     1501 ggcgcaaatt gtgctgcttg cttgggtaaa taactggctg gcaggcgatg gcgagctacc
     1561 gcagatgcct tccgttgcct ttggcgtaag ctgtgcattg gcggagatgg cagatacgct
     1621 cccgcaggcg gccaattacc gtgcggcacc actgtgtaat ggcgatcctg acgatctgat
     1681 cctcaaactt gctgatatgc caggcgagaa agtggcgaag gtcaaagtgg gattgtacga
     1741 agccgtgcgc gacggcatgg tggttaatct gttgctggag gcaattccgg atctgcattt
     1801 gcgtcttgac gcaaatcgcg cctggacacc gctgaaaggt cagcagtttg ccaaatatgt
     1861 taacccggat tatcgccacc gcatcgcgtt tctcgaagag ccgtgcaaaa cccgcgatga
     1921 ttcgcgagcg tttgcccgtg aaaccggcat tgccattgcc tgggatgaaa gcctgcgcga
     1981 gccggatttt gcctttgtgg ctgaagaggg cgtgcgcgcg gtagttatca aacccacgct
     2041 cacgggcagt ctggaaaaag tacgcgagca ggtacaggcg gcgcatgcgc tggggctgac
     2101 ggcggtgatc agttcttcca ttgagtcgag cttaggtcta acgcaactgg cgcggattgc
     2161 tgcctggtta acgccagaca ccattccggg actggacacg ctggatctga tgcaggcgca
     2221 gcaggttcgt cgctggccgg ggagcccact gccgttagtg gacgctgatg tactggagca
     2281 gttgttatga tcttctctga ctggccgtgg cgtcactggc ggcaagtgcg gggagaagcc
     2341 atcgccttac gtcttaatga cgagcaactc aactggcgcg agctttgtgc tcgcgtcgat
     2401 gagttagcta cgggctttgc ggcgcagggt gtggttgagg gcagcggcgt gatgttgcgg
     2461 gcgtggaata cgccgcaaac gctgctcgcc tggctggcgt tactgcaatg cggggcgcgg
     2521 gtgttgcccg tgaaccctca gctgccgcaa ccgttgcttg aagaattgct gcccaatctg
     2581 acgctgcaat ttgcgctggt gccggatggg gaaaacacgt ttccggcatt aacgtcgctg
     2641 cacattcagc gggttgaagg cgcacatgcc gctacgtggc agccgacgcg tctgtgctca
     2701 atgacgttga cctcgggttc taccggtttg ccgaaagccg ctgtccatac ttatcaggcc
     2761 catcttgcca gtgcgcaagg tgtgttatcg ctgattccgt ttggcgatca cgatgactgg
     2821 ttgctctctt taccgctgtt tcacgtctcc ggtcagggaa ttatgtggcg ctggttatac
     2881 gctggtgcgc ggatgacggt gcgtgataaa cagccattgg agcaaatgct ggcaggctgt
     2941 actcacgctt cactggtgcc aacacaactc tggcgtttgc tggttaaccg cagttccgtt
     3001 tccctgaaag cggtgttact tggcggcgcg gctatcccgg tcgagttgac ggaacaggcg
     3061 cgcgagcagg ggattcgttg cttttgcggc tatggtctga ccgagtttgc ctccacggtg
     3121 tgtgcgaaag aagccgacgg cctggcagac gttggttcgc cgctgccggg tcgggaagtg
     3181 aaaatcgtta ataatgaagt gtggctgcgg gctgccagta tggcagaagg ttactggcgt
     3241 aacgggcaac tggtttcact ggttaatgac gaaggctggt acgctacgcg cgatcgcggt
     3301 gagatgcata atggcaagct gaccattgtc ggacgtttag acaatctgtt cttcagtggc
     3361 ggagagggta ttcagccgga agaagtcgag cgcgtaattg ctgcacatcc tgcggttttg
     3421 caggtgttta tcgtccccgt tgccgacaag gagtttggtc atcgaccggt agcggtggtg
     3481 gagtatgacc agcaaaccgt tgatcttggc gaatgggtga aagataagct ggctcgtttt
     3541 caacaaccgg tgcgctggct aactttaccg ccggagctga aaaacggcgg tattaaaatt
     3601 tcacgtcagg cgttaaaaga gtgggtgcaa cgccaggatt acgaaatcat tagtttcact
     3661 tcgcaatttt gccattccat tgccgttgat aataggttta tctgttaatc tgtaaattga
     3721 tgtgaaaact cttagcaaac aggataatgc aatggaatgg ctggtcaaaa aatcgtgctg
     3781 caataaacaa gataacagac atgtacttat gctctgcgat gctggcggcg cgataaaaat
     3841 gatcgccgaa gtgaagagtg acttcgccgt gaaagtgggg gatttactct cgcctttgca
     3901 gaatgcgctt tattgtatta atcgtgaaaa gctgcatacg gtgaaagtcc tctcggcaag
     3961 cagttatagc cccgatgagt gggaacggca gtgtaaagtg gcagggaaaa ctcagtaacg
     4021 ttgttttgtc gtgggcagga aaatcagcat caacccgctc ataatacagg ctactcccag
     4081 tagtgctttc agcgaaaaag tcccttccca gccgggaagt accattgaag caatccatac
     4141 cagcacataa ctcatactga gcaaggcata ggctttgctt agcgcaagtt tatgtagcgt
     4201 tttataccaa caaaaaacgg aaagcagata acccagcagc ccgagtaaca gtatccgggc
     4261 atccaggcca aaagctaaca gagccgcgat aaaatcccat aagtgcgtca tcggcggcag
     4321 atgactcgcc gcaaaaccca ggcttaattg cgcaactgag gcaataatga cgctgaataa
     4381 tccccacatc aggcccatta caccgtactc ccgaggatca caatgccgcc aataatgaac
     4441 gccaccccac accagtgacg cggcgatacc ggttcgtgcc acagttttac tgcagccagc
     4501 gtcacccaga caaaattcag acttaacatc gggtaggcaa tgcctaccgg tacgttctgc
     4561 aagaccagca gccagagcac catggcaaga ccaagacaag ccagcgccag tcccagccac
     4621 agcacgatat gtttgcgccg tttgtttatc gccacaaagc aggttgcctg tttctgacac
     4681 aactgcccgg caacgctaag caagctggca aagactaatg ttagccagat catttgggac
     4741 gatactgaat cagcaccaga cgctcctgac gatcgatggc atctgcgggc ggaatggcga
     4801 gactgttgat atcttcatcg cggtcaaccg aaagcacgag tgtaataatc ccctcctgac
     4861 gatgttgatt aagccagttt gcgaactcat caccgctgac aaatctccct ttcgcatccg
     4921 gataattaag gccgtatttc aactcacctg tctggcgata catgatgatg tcatcgcgtt
     4981 gcaggctcca tgccagacct gcggcaacac cgacgctatc agtaagaata tagcggcttg
     5041 gctgaagtga ttcttgtgtc atctcgacaa aaaattgcgg atgttttcct tccatgactc
     5101 tgtcaggaat tgaaaatcct accagcaacg ccagccccag cgggcaaagc gcggcaaaag
     5161 accaggtctt ttcgacgttt gttaaggtgt accagccgaa aaatgcccat agcgaaaaaa
     5221 tcgaccaggc acaaaagact ttatagctct cgaaggtttg ccacaccggc gtgttcattg
     5281 gtccccacgg ggagaccaca aatgtggcaa taatgccagt gaagccaaaa gcgatgttaa
     5341 tccagccatt aatccgcagc gccagaggat tattttttgc tgccagcaaa gcgtaatgcg
     5401 ccatcagcat tgccagagat gcaaagcagg aaagaatata ggtgggcaat ttacctttag
     5461 cgacggagaa aaacagcagc ggcattatcg tccagctcaa caaatagacg gttgcggaat
     5521 gcttgcggtt tttccagcct gtgtacagtg caccggggag taatcccagc cacggcaggc
     5581 taccggcaat gatgaccggc acgtagtacc agaacggagc tctatgttgg gcatcatcca
     5641 gtgcaaagcg ttgaatatgc tcaacccaga aaaaatagtg ccagaagtca ggctcccgct
     5701 gcgctatcgc cagtccccag gggagaaccg tcagtacgca actgataacc gccagccagc
     5761 cgtaaataaa gagatctttc cagcgttttt gcgtcgctac ccatggcagc acgcttaata
     5821 ccggcacggc aagggcgaga aaacctttgg tcatcacccc catgccgcag gtgattccca
     5881 gcagtaaaaa tcctgcgctt ttgcctttcc acgtctgtgc ctgcattgcc agccagaagc
     5941 tgcacattcc cgccaccagc cagaatgcaa taaacggatc gagcacggca taagtgccga
     6001 tggcatagac aataaacaat gagagataaa ttactgtggc gagtagagcc agacgtttat
     6061 cgcgccataa gcgcagagta aaccaggtca ccagcgcggc agttaacagg gtcgcaaaga
     6121 taacgcctgc ccgcacacca aagttattcg cgccaaatag ccattgccca atgctgttaa
     6181 tccagtatcc ggcaatgggt ttttcgaaat aacgtagccc taacagatgg ggcacaatcc
     6241 agtcgccgga tgccagcatt tctcgactga tttccgcata acgcgtttca tcgggttgcc
     6301 agagcagacg cgtgctgatc ggtaacaggt aatagcaggc aataaacgcg aagaggccga
     6361 taaggtaacg taccgatttc atcagcgact acccgcaatt tgttggcaac ccagccagcc
     6421 ttcacgtccg gcaatatttc cgcgaacaac ttgtccgagc ggcaacgtct ctgataacag
     6481 ttcgcttaaa gggcaaaatg tcacgccttc ctgagctgcg cgtttgagga gatccacaaa
     6541 attatgctga taagcgcagc cttcaacttc tgcatgaatg gtataaaccg gcgtgccttt
     6601 atctcgcagg atgcggttga gtaaccaacc gttaaaatct tctgctttca catcccgacc
     6661 aatcacttca tcccaggtgg gtaaggtcac cggaatttgc gcagtgccag ggtttcctga
     6721 ttcgagcagt ggacggaacg gcatggcccc acgacaatcg ctgttgtagc gcaaatggaa
     6781 cgcttctttt gcttcgatca ccttctggtc ggcacgccag cccgcagcgg cagaacaggt
     6841 taccggttga ccgataatct cttccagagt gcgaagaccg cgtgcaatat cgtcgatcat
     6901 tgtttgccga tcccagttac cgctacgggc ttgccaggca tggtgatccc aggcgtgcag
     6961 gccgacttcg tgatgtttag ccgcttcacg aatgatatcg gcattggcat gaccaatctc
     7021 tttacctggc caggccgtac ctgccagtaa aatatcccag ccataaagcg atgccgcgtt
     7081 tgagcgcagc atcttccaca aaaactgtgg cttcaccagt cgccagagat ggcggcccat
     7141 attgtccggg ccgacgctga aaaaaatgct ggcctgaata ttatgcttac tcaagatttc
     7201 cagcagacgt ggcacgcctt cacgggtgcc acgaaaggta tcgacatcaa tgcgtaagcc
     7261 gactttggtc atgatggttt atccgtaaga tcaacggtgc gcaggaagaa atccagcgtt
     7321 tcgtcgatgg tttcctgcat atcaattttc ggttcccagt ccaggcagcg gcgggcattg
     7381 cggatgctcg gtttacgatg ctctacgtcc tgatatcctt tgccgtagta gctgctactt
     7441 tcgacaacac gaaagcccgc aaacggcggg aagtgatggc gcagcggatg tttttcgaag
     7501 ctcgccagca gcatctcgcc cagttcctca atgctcgctt cgttctcagg attgccaatg
     7561 ttgataattt cgccgtcgca gcgatttccc gcattttcga taatgcggta caacgcctcg
     7621 ataccgtccc ggatatcagt aaagcagcgt ttttgttttc cgccatcaat tagcttaatt
     7681 ggtgaacctt ctaccagatt gagaatgagt tgcgtaatag cgcgggagct gccaattcgc
     7741 gctgcattaa ggttatccag tcgtggtccc atccagttaa acgggcggaa gagggtgaac
     7801 tgtaaaccct ctttttcgcc ataggcccag atcacccgat caagtaattg ttttgacacc
     7861 gaataaatcc agcgtggttt attcaccggg ccgacgatta aattagaatg gtcctcatcg
     7921 aagtatttat cgctacacat cccataaact tctgaagttg acgggaagat gattcgctta
     7981 cggtacttaa cgcagtagcg gataatgcgc agattctctt caaaatcgag ttcaaatacg
     8041 cgcagcgggt tgcgggtata ttcaatcggc gtggctatcg ccaccagcgg caagacgaca
     8101 tcacattttt tgacgtgata ctcaatccat tcggaatgaa tactgatatc gccttcgaca
     8161 aagtgaaaat gcggatgatt cagaaaacgg cttatcgcat cgctgccaat atccagaccg
     8221 taaacttcat aatgatcttc gcgcagcagg cgttctgtca gatggttgcc aataaagcca
     8281 ttcaccccga ggatgagtac ccgggtacgg cgtcgggcgg tgcaggcagg ctggctattc
     8341 aagcgtgaac cttgcaccag gcccagcatc tgcgctaatt gcgagccctg catagtaatg
     8401 ccgtcgcccg cctgtccggt gacgatttcc agcgcgccat cgccacaggc aatcagcagt
     8461 ggcgcaacag aaatcacgct ccccggctgt gctttgctgg catgaggatg aacacgcgac
     8521 gaccagacgg tgaatttctg attgccaaca tagctgaagg cacccggcca cggatcggca
     8581 acggcacgta ccatgttgtg cagtacggat gccggtttat gccattcaag gaaactgtca
     8641 tctggcgttc tgtgaccaaa acaggtggct tcgttttcgc gctgggcaat ttccagaata
     8701 ttaccgtgtt taatggcggg taatgtctgt tccagtagct ggcgcgcggc atggcacaat
     8761 ttatgatgca gcgtaatagc gatatcgtct ggcgcaatgg caacgcgcag ttgcgccaca
     8821 atggccccag catcggcacg ttttaccatt cggtgcaatg taacgcccgt ttccgtttca
     8881 ccgttcacca gcacccagtt cagcggcgcg cgaccacgat attttggtaa cagcgaaccg
     8941 tgcagattaa atgcacctgc gggagcgagc tgcaaaatct cgtcgcaaat aagatggcga
     9001 taataaaaag agaaaatcac ctctggtgag agttgggcaa tgcgttccac ccacagcgga
     9061 tgattaacgt tatccggcgc ataaactgga atgcctcttt ccgccgccag acgagccacc
     9121 gaaccataaa aggctttttc agcgggatta tcggtatggg taaaaatggc gctaatttcg
     9181 taaccggcag ccagcagagc ttcaataccg aggcatccca tatcgtggta ggcaaaaacg
     9241 acggttttca ttattcattt tccttgctgg atggacggat aacttgctga acaaaatagc
     9301 gggggcgggc gcggacatcg gtgtagatcc tgccgatata ttcaccgagc aatcccatgc
     9361 cgataaactg agcgccaata aaagtaaaca gcacggcaaa tagcataaag acgccttctg
     9421 ccgcccattg tggtccgaag gtcaggcgta aaatcaccag cagcaccgca atgctaaaac
     9481 ctccaatcgc aataatgctg ccgagcagac tcagcatacg tagcggcgtt gtggtaaggc
     9541 aggtcaccag gtcgtacatc aaattaatca ggcgcataaa actgtatttg gattcaccaa
     9601 actcacgctc ggcatgatgt actggaattt caatggcacg gcgggcgaag atattcgcca
     9661 gaatcgggat aaaggtgcta cgttcatggc agtgcaacat cgcatcgaca atatgacggc
     9721 gataggcgcg cagcatacaa ccgtagtcac ccatcgcttt gccggtggtg cgctgaataa
     9781 gcctgttaat catcttcgaa gctgttttac gaaaccagct gtcctggcgg ttctggcgta
     9841 cagtccctac cacgtcgtaa ccttcatcgg cttttgccac caggcggggg atttcttctg
     9901 gcggattctg gagatcggca tcaagggtaa taattaagtc gccagtaacg tgactgaatc
     9961 ccgccataat cgctgaatgt tgcccgtaat tgcggttaag caaaatagac acaatatggc
    10021 tgttctccgc ttgtgaggct tcgaccagca tatgcgcgga attatcgcta ctgccgtcat
    10081 caatcagcag gatctcatac tctttcccca acgattcaca ggctgtggtg gtgcgcctga
    10141 ttaattccgg taagctttcc tgctcgttat aaacgggaat aaccaccgag actttcttaa
    10201 cagggtggat ttcaaacatg gcttattgtc ctgcgagttg ctgaagggct gtgatgacac
    10261 ggtcggcatc ggcggtagtc atatccggga acagcggcaa agaacagatg cgttcgctat
    10321 tccattcggt attcggtaac gacagcgtgg gaaaacgctc gcgataatat ttttgtgtgt
    10381 gagcggcgcg gaaatgtaac ccggtaccaa tgcctctttc ttttaacgct tccatcaacg
    10441 catcgcgact gataccacaa cgttgttcat cgacacgaat aataaacaga tgccaggcgt
    10501 gaacgtgcgg ccaggcggga aggcttaatg gctgaaaggg gagagctgcc agtgcttgct
    10561 gatattgctg ggcaatttcg cgccgatggg tgttgaggtg ctctaatttt gctaactgtg
    10621 tcagggcaat cgcggcgtta atatcggtca gattgtactt atagcccggt gttaagactt
    10681 cagcctgcgg tgcacggccc caggtttgtc tgtcataggc atcgacaccc agaccgtgaa
    10741 atttcagcat ccgtagctgg cgggcaaggt tttcattatc agttacaatc aggccacctt
    10801 ccgcacaggt aatattttta atggcatgaa atgaaaaaat agcggtacct tttgcgccaa
    10861 tatgtcgccc tttgtaatac gtaccgacgg catgggcagc atcttcgata actgcgatgc
    10921 cgtaacgttc gccaatggcg cgaatggcgt caatatctgc tggcgcaccg gcataatgca
    10981 ccggaatgat ggctttagtg cgtggcgtaa tggctgactc gatcgcttca ggcgtgacca
    11041 tcagcgtatc gcggtcgaca tccaccatta ccggcgttgc acccaacaag gaaatcatat
    11101 tgagggttga aacccaggtc agggaaggcg taatcacttc atcgcccttg ccaatttcca
    11161 acgccattag cgtgatatgc attccggcgg tggctgaact gaccgcgatg gcatgctgat
    11221 ttcccgtcaa ctggcaaaag gcttgctcaa gcgcctgatt cttcggaccg gttgtgatcc
    11281 aaccggattc gagaacctct ttaactgcag cgagttcctc cacgcccatt gctggtcgcg
    11341 aaaaaggcaa aaattctgac attgcttttc cttccgccat tgaatacctg tccacttata
    11401 tttgctataa agagtgt
//
LOCUS       DAZSBF010000128        11355 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZSBF010000128 DAZSBF010000000
VERSION     DAZSBF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.129"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHXWD_002717"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="ACHXWD_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR7621599.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="ACHXWD_002718"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="ACHXWD_002718"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR7621600.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="ACHXWD_002719"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="ACHXWD_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR7621601.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHXWD_002720"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="ACHXWD_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR7621602.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="ACHXWD_002721"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="ACHXWD_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR7621603.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="ACHXWD_002722"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="ACHXWD_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR7621604.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="ACHXWD_002723"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="ACHXWD_002723"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR7621605.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="ACHXWD_002724"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="ACHXWD_002724"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR7621606.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHXWD_002725"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="ACHXWD_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR7621607.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="ACHXWD_002726"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="ACHXWD_002726"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR7621608.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="ACHXWD_002727"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="ACHXWD_002727"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR7621609.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="ACHXWD_002728"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="ACHXWD_002728"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR7621610.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="ACHXWD_002729"
     CDS             10898..>11355
                     /locus_tag="ACHXWD_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR7621611.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2873 a         2631 c         3055 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtact gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAZSBF010000129        11285 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZSBF010000129 DAZSBF010000000
VERSION     DAZSBF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.130"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            329..1897
                     /gene="cydA"
                     /locus_tag="ACHXWD_002730"
     CDS             329..1897
                     /gene="cydA"
                     /locus_tag="ACHXWD_002730"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR7621612.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1913..3052
                     /gene="cydB"
                     /locus_tag="ACHXWD_002731"
     CDS             1913..3052
                     /gene="cydB"
                     /locus_tag="ACHXWD_002731"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR7621613.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3067..3180
                     /gene="cydX"
                     /locus_tag="ACHXWD_002732"
     CDS             3067..3180
                     /gene="cydX"
                     /locus_tag="ACHXWD_002732"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR7621614.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3180..3473
                     /gene="ybgE"
                     /locus_tag="ACHXWD_002733"
     CDS             3180..3473
                     /gene="ybgE"
                     /locus_tag="ACHXWD_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR7621615.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3623..4027
                     /gene="ybgC"
                     /locus_tag="ACHXWD_002734"
     CDS             3623..4027
                     /gene="ybgC"
                     /locus_tag="ACHXWD_002734"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR7621616.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            4024..4716
                     /gene="tolQ"
                     /locus_tag="ACHXWD_002735"
     CDS             4024..4716
                     /gene="tolQ"
                     /locus_tag="ACHXWD_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR7621617.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4720..5148
                     /gene="tolR"
                     /locus_tag="ACHXWD_002736"
     CDS             4720..5148
                     /gene="tolR"
                     /locus_tag="ACHXWD_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR7621618.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5213..6445
                     /gene="tolA"
                     /locus_tag="ACHXWD_002737"
     CDS             5213..6445
                     /gene="tolA"
                     /locus_tag="ACHXWD_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR7621619.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            6578..7870
                     /gene="tolB"
                     /locus_tag="ACHXWD_002738"
     CDS             6578..7870
                     /gene="tolB"
                     /locus_tag="ACHXWD_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR7621620.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7905..8426
                     /gene="pal"
                     /locus_tag="ACHXWD_002739"
     CDS             7905..8426
                     /gene="pal"
                     /locus_tag="ACHXWD_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR7621621.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8436..9227
                     /gene="cpoB"
                     /locus_tag="ACHXWD_002740"
     CDS             8436..9227
                     /gene="cpoB"
                     /locus_tag="ACHXWD_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR7621622.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9392..9467
                     /locus_tag="ACHXWD_002741"
     tRNA            9392..9467
                     /locus_tag="ACHXWD_002741"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9425..9427,aa:Lys,seq:ttt)
     gene            9617..9692
                     /locus_tag="ACHXWD_002742"
     tRNA            9617..9692
                     /locus_tag="ACHXWD_002742"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9650..9652,aa:Val,seq:tac)
     gene            9696..9771
                     /locus_tag="ACHXWD_002743"
     tRNA            9696..9771
                     /locus_tag="ACHXWD_002743"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9729..9731,aa:Lys,seq:ttt)
     gene            9820..9895
                     /locus_tag="ACHXWD_002744"
     tRNA            9820..9895
                     /locus_tag="ACHXWD_002744"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9853..9855,aa:Lys,seq:ttt)
     gene            9929..10004
                     /locus_tag="ACHXWD_002745"
     tRNA            9929..10004
                     /locus_tag="ACHXWD_002745"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9962..9964,aa:Lys,seq:ttt)
     gene            10035..10160
                     /locus_tag="ACHXWD_002746"
     ncRNA           10035..10160
                     /ncRNA_class="other"
                     /locus_tag="ACHXWD_002746"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10409..>11285
                     /gene="nadA"
                     /locus_tag="ACHXWD_002747"
     CDS             10409..>11285
                     /gene="nadA"
                     /locus_tag="ACHXWD_002747"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR7621623.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2783 a         2707 c         3027 g         2768 t
ORIGIN      
        1 agtcttggaa attggcatca aaaagagata aattgttctc gatcaaattg gctgaaaggc
       61 ggtaatttag ctataaattg atcaccgtcg aaaaatgcaa atttgcttca acaaaaacct
      121 gtttattgta aggattttgc agcgtaatat atacgtggga tcaatttgag tttttattaa
      181 catgtttgca acctttcttt acgccgtttt tgtgtgcatt cacatggtat gatgaaagtg
      241 ttcaaacaaa tttctattgg ggcatgcgtg tgaccctttc taacggggtt cactctcgga
      301 gtcttcatgc gatgagcaag gagtcatgat gttagatata gtcgaactgt cgcgcttaca
      361 gtttgccttg accgcgatgt accacttcct ttttgtgcca ctgacgctcg gtatggcgtt
      421 cctgctggcc attatggaaa cggtctacgt cctctccggc aaacagattt ataaagatat
      481 gaccaagttc tggggcaagt tgtttggtat caacttcgct ctgggtgtgg ctaccggtct
      541 gaccatggag ttccagttcg ggactaactg gtcttactat tcccactatg taggggatat
      601 cttcggtgcg ccgctggcaa tcgaaggtct gatggccttc ttcctcgaat ccacctttgt
      661 aggtctgttc ttcttcggtt gggatcgtct gggtaaagtt cagcatatgt gtgtcacctg
      721 gctggtggcg ctcggttcaa acctgtccgc actgtggatt ctggttgcga acggctggat
      781 gcaaaaccca atcgcgtccg atttcaactt tgaaactatg cgtatggaga tggtgagctt
      841 ctccgagctg gtgcttaacc cggttgctca ggtgaaattc gttcacactg tagcgtctgg
      901 ttatgtgact ggcgcgatgt tcatcctcgg tatcagcgca tggtatatgc tgaaaggtcg
      961 tgacttcgcc ttcgctaaac gctcctttgc tatcgctgcc agcttcggta tggctgctgt
     1021 tctgtctgtt attgttctgg gtgatgaatc cggctacgaa atgggcgacg tgcagaaaac
     1081 caaactggct gctattgaag ccgagtggga aacgcaacct gcgcctgctg cctttactct
     1141 gttcggcatt cctgatcagg aagaggagac gaacaaattt gcgattcaga tcccttacgc
     1201 actgggcatc attgcaacgc gttccgtgga taccccggtt atcggcctga aagagctgat
     1261 ggtgcagcat gaagaacgca ttcgtaacgg gatgaaggcg tactctctgc tcgaacaact
     1321 gcgttctggt tctaccgacc aggcggttcg tgaccagttc aatagcatga agaaagacct
     1381 cggttacggt ctgctgctga aacgctatac gccaaacgtg gctgatgcga ctgaagcgca
     1441 gattcaacag gcaaccaaag actccatccc gcgtgtagcg ccgctgtact ttgcgttccg
     1501 tatcatggtg gcgtgtggct tcctgcttct ggcaatcatc gcgctctctt tctggagtgt
     1561 catccgcaac cgcattggcg agaaaaaatg gcttctgcgc gccgcgctgt acggtattcc
     1621 gctgccgtgg attgctgtag aagcgggctg gttcgtggct gaatatggcc gccaaccgtg
     1681 ggctatcggt gaagtgctgc cgacagctgt ggcgaactcg tcactgaccg caggcgatct
     1741 catcttctca atggtgctga tttgcggcct gtataccctg ttcctggtgg cagaattgtt
     1801 cttaatgttc aagtttgcac gcctcggccc aagcagcctg aaaaccggtc gctatcactt
     1861 tgagcagtct tccacgacta ctcagccggc acgctaagac aggagtcgtc aaatgatcga
     1921 ttatgaagta ttgcgtttta tctggtggct gctggttggc gttctgctga ttggttttgc
     1981 agtcactgac ggtttcgaca tgggggtggg catgctcacc cgtttcctcg gtcgtaacga
     2041 caccgagcgt cgaattatga ttaactccat tgcaccacac tgggacggta accaggtttg
     2101 gctgatcacc gcgggcggcg cactctttgc tgcctggccg atggtctatg ccgctgcgtt
     2161 ctccggcttc tatgtggcga tgatcctcgt gctggcgtct ttgttcttcc gtccggtcgg
     2221 ttttgactac cgctccaaga ttgaagaaac ccgctggcgt aacatgtggg actggggcat
     2281 cttcattggt agtttcgttc cgccgctggt aattggtgta gcgttcggta acctgttgca
     2341 gggcgtaccg ttcaacgttg atgaatatct gcgtctgtac tacaccggta acttcttcca
     2401 gttgcttaac ccgttcggcc tgctggcagg cgtggtgagc gtagggatga tcattactca
     2461 gggcgcaacc tatctgcaaa tgcgtaccgt gggcgaactg cacctgcgta cccgtgcaac
     2521 ggctcaggtg gctgcgctgg tgacactggt ctgtttcgca ctggctggcg tatgggtgat
     2581 gtacggtatc gatggttatg tcgtgaaatc gacaatggac cattacgcag cctctaaccc
     2641 actgaataaa gaagtggttc gtgaagctgg cgcatggctg gttaacttca acaacacgcc
     2701 aattctgtgg gctattccgg cactgggtgt ggttcttccg ctgctgacca tcctgactgc
     2761 acgtatggat aaagccgcgt gggcgtttgt gttctcctcc ctgacgctgg cctgcatcat
     2821 cctgacagcc ggtatcgcaa tgttcccgtt tgtgatgccg tccagcacca tgatgaacgc
     2881 aagtctgaca atgtgggatg caacttccag ccagctgacg cttaacgtca tgacctgggt
     2941 tgcggtggtt ctggtaccga tcattctgct ctacaccgct tggtgttact ggaaaatgtt
     3001 cggtcgtatc accaaagaag atattgaacg taacacccac tctctgtact aagtaaggag
     3061 ctaaaaatgt ggtatttcgc atggattctg ggaacgcttc ttgcctgttc gtttggggta
     3121 atcaccgcgc tggcgcttga acacgtcgaa tcaggcaaag ccggtcaaga agacatctga
     3181 tgagtaagat tatcgcgacc ttgtatgcgg taatggacaa gcgccccctg cgggcgcttt
     3241 ccttcgtgat ggcgcttctg ttagcaggat gtatgttttg ggacccatct cgttttgccg
     3301 cgaagaccag tgaactggaa atctggcatg gtttattgct gatgtgggcc gtctgtgctg
     3361 gtgtgattca cggcgtgggc tttcgtccgc agaaggttct ttggcaaggg attttttgcc
     3421 cattgcttgc cgatattgtt ctcattgtcg ggctgatttt cttcttcttt taaatcagaa
     3481 ttttctttaa aaaattatgg gccgctccag gcccataaat ttttacgctc ccttaacttg
     3541 ccctcattcc caaacctcaa tcgcgcgcgt atagtagcag cgtttaaaag ctctaacttt
     3601 tgttgcatta ccgggatgta aagtgaatac aacgctgttt cgatggccgg ttcgcgtcta
     3661 ctatgaagat accgatgccg gtggtgtggt gtaccacgcc agttacgtcg ctttttatga
     3721 aagagcacgc acagagatgc tgcgtcatca tcacttcagt cagcaggcgc tgatggctga
     3781 acgcgttgcc tttgtggtac gtaaaatgac ggtggaatat tacgcacctg cgcggctcga
     3841 cgatatgctc gaaatacaga ctgaaataac atcaatgcgt ggcacctctt tggttttcac
     3901 gcaacgtatt gtcaacgccg agaatacttt gctgaatgaa gcagaggttc tggttgtttg
     3961 cgttgaccca ctcaaaatga agccttgtgc gcttcccaag tctattgtcg cggagtttaa
     4021 gcagtgactg acatgaatat ccttgatttg ttcctgaagg ctagccttct ggttaaactt
     4081 atcatgttga ttttgattgg tttttcaatc gcatcctggg ccattattat ccagcggacc
     4141 cgtattctta acgcagcggc gcgcgaagcc gaagcgtttg aagataaatt ctggtctgga
     4201 atcgaactct ctcgcctcta tcaagagagt caggggaaac gggataatct gactggttcg
     4261 gaacaaatat tttacagcgg gttcaaagag tttgtgcgcc tgcatcgtgc caatagccat
     4321 gcgccggaag ccgtagtgga aggggcgtcg cgtgctatgc gtatctccat gaaccgtgaa
     4381 cttgaaaatc tggaaacgca cattccgttc ctcggtacgg ttggctccat cagcccgtat
     4441 attggtctgt ttggtacggt ctgggggatc atgcacgcct ttatcgccct cggggcggta
     4501 aaacaagcaa cactgcaaat ggttgcgccc ggtatcgcag aagcgttgat tgcgactgca
     4561 attggtctgt tcgccgcaat tccggcggta atggcttata accgcctcaa ccagcgcgta
     4621 aacaaactgg aactgaatta cgacaacttt atggaagagt ttaccgcgat tctgcaccgc
     4681 caggcgttta ccgttagcga gagcaacaag gggtaagcca tggccagagc gcgtggacga
     4741 ggtcgtcgcg atctcaagtc cgaaatcaac attgtaccgt tgctggacgt actgctggtg
     4801 ctgttgctga tctttatggc gacagcgccc atcatcaccc agagcgtgga ggtcgatctg
     4861 ccagacgcta ctgaatcaca ggcggtgagc agtaacgata atccgccagt gattgttgaa
     4921 gtgtctggta ttggtcagta caccgtggtg gttgagaaag atcgcctgga gcgtttacca
     4981 ccagagcagg tggtggcgga agtgtccagc cgtttcaagg ccaacccgaa aacggtcttt
     5041 ctgatcggtg gcgcaaaaga tgtgccttac gatgaaataa ttaaagcact gaacttgtta
     5101 catagtgcgg gtgtgaaatc ggttggttta atgacgcagc ctatctaaac atctgcgttt
     5161 cccttgcttg aaagagagcg ggtaacaggc gaacagtttt tggaaaccga gagtgtcaaa
     5221 ggcaaccgaa caaaacgaca agctcaagcg ggcgataatt atttcagcag tgctgcatgt
     5281 catcttattt gcggcgctgc tctggagttc gttcgatgag aatatagaag cttcagccgg
     5341 aggcggcggt ggttcgtcca tcgacgctgt catggttgat tcaggtgcgg tagttgagca
     5401 gtacaaacgc atgcaaagcc aggaatcaag cgcgaagcgt tctgatgagc agcgcaagat
     5461 aaaggaacag caggctgctg aagaactgcg tgagaaacaa gcggctgaac aggaacgcct
     5521 gaagcaactt gagaaagagc ggttagctgc tcaggaacag aaaaagcagg ctgaagaagc
     5581 cgcaaaacag gccgagttaa agcagaagca agcggaagag gcggcagcga aagcggcggc
     5641 agatgctaaa gcgaaggccg aagcggatgc taaagctgcg gaagaagcag cgaagaaagc
     5701 ggcggcagac gcgaagaaaa aagcagaagc agaagccgcc aaagccgcag ccgaagcgca
     5761 gaaaaaagcc gaggcagccg ctgcggcact gaagaagaaa gcggaagcgg cagaagctgc
     5821 tgcagctgaa gcaagaaaga aagcggcagc agagaaagct gcagccgaca aaaaagcagc
     5881 agaaaaagcg gctgctgata aggcagcagc tgataagaaa gcagcggcag aaaaagccgc
     5941 cgcagacaaa aaagcggcag cggcaaaagc agcggcagaa aaagccgccg cagacaaaaa
     6001 agcggcagcg gcaaaagcag cagctgaaaa agccgctgca gcaaaagctg ccgcagaggc
     6061 agatgatatt ttcggtgagc taagctctgg taagaatgca ccgaaaacgg ggggaggggc
     6121 gaaagggaac aatgcttcgt ctgccgggag tggtaatact aaaaacaatg gcgcatcagg
     6181 ggccgatatc aataactatg ccgggcagat taaatctgct atcgaaagta agttctatga
     6241 cgcatcgtcc tatgcaggca aaacctgtac gctgcgcata aaactggcac ccgatggtat
     6301 gttactggat atcaaacctg aaggtggcga tcccgcactt tgtcaggctg cgttggcagc
     6361 agctaaactt gcgaagatcc cgaaaccacc aagccaggca gtatatgaag tgttcaaaaa
     6421 cgcgccattg gacttcaaac cgtaatcgcg atgttgactg ttccgacggt caacatcagg
     6481 caccggttgc cacggggttc tggtagtttt gtgtatttta gtttgttaac attctgctaa
     6541 attatcgtgg gccatcggtc cagataaggg agatatgatg aagcaggcat tacgagtagc
     6601 atttggtttt ctcatactgt gggcatcagt tctgcatgct gaagtccgca ttgtgatcga
     6661 cagcggtgta gattccggtc gtcctattgg tgttgttcct ttccagtggg cgggccctgg
     6721 tgcggcacct gaagatattg gcggcatcgt tgctgccgac ttgcgtaaca gcggtaaatt
     6781 taatccgtta gatcgcgctc gtctgccaca gcagccgggt agtgcgcagg aagtacaacc
     6841 agctgcatgg tccgcgctgg gtattgacgc tgtagttgtc ggtcaggtca ctccgaatcc
     6901 ggatggttct tacaatgttg cttatcaact tgttgacact ggcggcgcac cgggtactgt
     6961 acttgctcag aactcgtaca aagtgaacaa gcagtggctg cgctatgctg gtcataccgc
     7021 cagtgatgaa gtgtttgaaa aactgaccgg cattaaaggt gcgttccgta cccgtattgc
     7081 ctacgttgtt cagaccaacg gcggtcagtt cccgtatgaa ctgcgcgtat ctgactatga
     7141 cggttacaac cagtttgtcg ttcaccgttc accacagccg ctgatgtctc cggcgtggtc
     7201 accagacggt tctaaactgg cttatgtgac cttcgaaagc ggtcgttccg cgctagttat
     7261 ccaaacgctg gcaaacggcg ctgtacgtca ggtggcttca ttcccgcgtc acaacggtgc
     7321 gcctgcattc tcgccagacg gcagcaaact ggcattcgcc ttgtcgaaaa ccggtagcct
     7381 gaacctgtac gtaatggatt tggcttctgg tcagatccgc caggtgactg atggtcgcag
     7441 taacaatacc gaaccgacct ggttcccgga cagccagaac ctggcattta cttctgacca
     7501 ggccggtcgt ccacaggttt ataaagtgaa tatcaacggc ggtgcgccac aacgtattac
     7561 ctgggaaggt tcgcagaacc aggatgcgga tgtcagcagc gacggtaaat ttatggtaat
     7621 ggtcagctcc aacggtgggc agcagcacat tgccaaacaa gatctggcaa cgggaggcgt
     7681 acaagttctg tcgtccacgt tcctggatga aacgccaagt ctggcaccta acggcactat
     7741 ggtaatctac agctcttctc aggggatggg atccgtgctg aatttggttt ctacagatgg
     7801 gcgtttcaaa gcgcgtcttc cggcaactga tggacaggtc aaattccctg cctggtcgcc
     7861 gtatctgtga taataattaa ttgaatagta aaggaatcat tgaaatgcaa ctgaacaaag
     7921 tgctgaaagg gctgatgatt gctctgcctg ttatggcaat tgcggcatgt tcttccaaca
     7981 agaacgccag caatgacggc agcgaaggca tgctgggtgc cggcactggt atggatgcga
     8041 acggcggcaa cggcaacatg tcttccgaag agcaggctcg tctgcaaatg caacagctgc
     8101 agcagaacaa catcgtttac ttcgatctgg acaagtacga tatccgttct gacttcgctc
     8161 aaatgctgga tgcacatgca aacttcctgc gtagcaaccc gtcttacaaa gtcaccgtag
     8221 aaggtcacgc ggacgaacgt ggtactccgg aatacaacat ctccctgggt gaacgtcgtg
     8281 cgaacgccgt taagatgtac ctgcagggta aaggcgtttc tgcagaccag atctccatcg
     8341 tttcttacgg taaagaaaaa cctgcagtac tgggtcatga cgaagcggca tactccaaaa
     8401 accgtcgtgc ggtactggtt tactaagaga attgcatgag cagtaacttc agacatcaac
     8461 tattgagtct gtcgttactg gttggtatag cggccccctg ggccgctttt gctcaggcac
     8521 caatcagtag tgtcggctca ggctcggtcg aagaccgcgt cactcaactt gagcgtattt
     8581 ctaatgctca cagccagctt ttaacccaac tccagcaaca actttctgat aatcaatccg
     8641 atattgattc cctgcgtggt cagattcagg aaaatcagta tcaactgaat caggtcgtgg
     8701 agcggcaaaa gcagatcctg ttgcagatcg acagcctcag cagcggtggt gcagcggcgc
     8761 aatcaaccag cagcgatcaa agcggtgcgg cggcatcaac gacgccgaca gctgatgctg
     8821 gtactgcgaa tgctggcgcg ccggtgaaaa gcggcgatgc aaacacggat tacaatgcag
     8881 ctattgcgct ggtgcaggat aaatcccgcc aggatgacgc aatggtggca tttcagaatt
     8941 tcatcaaaaa ttaccctgat tcaacttacc tgccaaacgc caattattgg ctgggtcagt
     9001 taaactacaa caagggtaaa aaagatgatg cggcgtacta ttttgcttcg gtagtgaaaa
     9061 actatccgaa gtcaccaaag gctgctgatg cgatgtttaa agtcggcgtc atcatgcagg
     9121 acaaaggtga caccgcaaaa gcgaaagccg tataccagca ggttatcagt aaataccctg
     9181 gtactgatgg cgctaaacag gcacaaaaac gtctgaacgc gatgtaatgc ataacacacg
     9241 accagaagtc gcattatttc tggtcgtgtc gtgcgaatca taagcagttg agtgatctgc
     9301 atcgaaattt ttgttgcgct caagtctgaa atcagtaata tatgccgccg ttgccacggg
     9361 atatcaaaca aaccgaaagc aacgaaaaag tgggtcgtta gctcagttgg tagagcagtt
     9421 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccagtt ttagcatcga
     9481 agacagatgt taagcgtgta ggataacgtt gcgtcagcaa cggcccgtag ggcgagcgaa
     9541 gcgagtcatc ctgcacgacc caccactaat tacggtgggt tcggtggaag tagtttgtag
     9601 tatccagcgc agtatcgggt gattagctca gctgggagag cacctccctt acaaggaggg
     9661 ggtcggcggt tcgatcccgt catcacccac cactcgggtc gttagctcag ttggtagagc
     9721 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatcttaaag
     9781 attggccccg agtagaaatc tttcaggtaa cacccgtatg ggtcgttagc tcagttggta
     9841 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccatcctg
     9901 aatgattaaa gcagcataat cccgcaaggg gtcgttagct cagttggtag agcagttgac
     9961 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa aaaagcgccc
    10021 taaaggcgct tttttgctat ctgcgatact caaagattcg aacctgcagc aggtttgagt
    10081 tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga gcgaagcgag
    10141 tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt tgctattcag
    10201 gcatcctcaa tttcactttg taaacctgat gacatcgtca gagcttactg tgcaagcaac
    10261 tctacgtcgg tggaattagg cgtaattcga gtgacttttc cccaccattc gactatcttg
    10321 tttagcatat aaaacaaatt acgccgatag tagcgaatat tacgctaatg tcggttttaa
    10381 cgttaagcct gtaaaacgag atggtaagat gagcgtgatg tttgatccag acacggcgat
    10441 ttatcctttc cccccgaagc cgacgccgtt aagcattgat gaaaaagcgt attaccgcga
    10501 gaagataaaa cgtctgctaa aagaacgtaa tgcggtgatg gttgcccact actataccga
    10561 ccccgaaatt caacaactgg cagaagaaac cggtggctgt atttctgatt ctctggaaat
    10621 ggcgcgcttc ggtgcaaagc atcccgcttc tacgttgtta gtcgctgggg tgagatttat
    10681 gggagaaacc gccaaaattc tcagtccgga aaaaacaatt ctgatgctga cacttcaggc
    10741 tgaatgttca ctggatctcg gctgccctgt tgaagaattt aacgcatttt gcgatgccca
    10801 tcccgatcat actgtcgtcg tctacgccaa cacttctgct gcggtaaaag cgcgcgcaga
    10861 ttgggtggta acttcaagca ttgccgtcga acttattgat catctggata gttttggtga
    10921 aaaaatcatc tgggcacccg acaaacatct ggggcgttac gtgcaaaaac agacgggcgc
    10981 agacatttta tgctggcagg gtgcctgtat tgtgcatgat gaatttaaga ctcaggcgtt
    11041 aacccgcttg caagaagaat acccggatgc tgccatactg gtgcatccag aatcaccaca
    11101 agctattgtc gatatggcgg atgcggtcgg ttccaccagt caactgatcg ctgctgcgaa
    11161 aacattgcca catcagcggc ttatcgtggc aaccgatcgg ggcattttct acaaaatgca
    11221 gcaggcggtg ccagataaag agttactgga agcaccaacc gcaggtgagg gtgcaacctg
    11281 ccgca
//
LOCUS       DAZSBF010000130        11243 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZSBF010000130 DAZSBF010000000
VERSION     DAZSBF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.131"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHXWD_002748"
     CDS             3..308
                     /locus_tag="ACHXWD_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR7621624.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHXWD_002749"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHXWD_002749"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR7621625.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHXWD_002750"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHXWD_002750"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR7621626.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHXWD_002751"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHXWD_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR7621627.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHXWD_002752"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHXWD_002752"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR7621628.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHXWD_002753"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHXWD_002753"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR7621629.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHXWD_002754"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHXWD_002754"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR7621630.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHXWD_002755"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHXWD_002755"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR7621631.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHXWD_002756"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHXWD_002756"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR7621632.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHXWD_002757"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHXWD_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR7621633.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHXWD_002758"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHXWD_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR7621634.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11243
                     /locus_tag="ACHXWD_002759"
                     /pseudo
     CDS             10353..11243
                     /locus_tag="ACHXWD_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2949 g         2650 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtgtcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cgt
//
LOCUS       DAZSBF010000131        11211 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZSBF010000131 DAZSBF010000000
VERSION     DAZSBF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.132"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1285
                     /gene="narU"
                     /locus_tag="ACHXWD_002760"
     CDS             <1..1285
                     /gene="narU"
                     /locus_tag="ACHXWD_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR7621635.1"
                     /translation="KTITYLSDTGCLEIQGASLAVTVNLNKIGFNFTTDQLFLLTALP
                     SVSGALLRVPYSFMVPIFGGRRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALL
                     CGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFL
                     GVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLH
                     LWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISD
                     KFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQM
                     IAVIFRQITIYRVKMKGGSDEQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLN
                     MTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1367..5107
                     /gene="narZ"
                     /locus_tag="ACHXWD_002761"
     CDS             1367..5107
                     /gene="narZ"
                     /locus_tag="ACHXWD_002761"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR7621636.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5104..6648
                     /gene="narH"
                     /locus_tag="ACHXWD_002762"
     CDS             5104..6648
                     /gene="narH"
                     /locus_tag="ACHXWD_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR7621637.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6648..7343
                     /gene="narW"
                     /locus_tag="ACHXWD_002763"
     CDS             6648..7343
                     /gene="narW"
                     /locus_tag="ACHXWD_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR7621638.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7340..8020
                     /gene="narI"
                     /locus_tag="ACHXWD_002764"
     CDS             7340..8020
                     /gene="narI"
                     /locus_tag="ACHXWD_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR7621639.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8099..8992
                     /locus_tag="ACHXWD_002765"
     CDS             8099..8992
                     /locus_tag="ACHXWD_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR7621640.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9088..9933)
                     /gene="nhoA"
                     /locus_tag="ACHXWD_002766"
     CDS             complement(9088..9933)
                     /gene="nhoA"
                     /locus_tag="ACHXWD_002766"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR7621641.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10106..10675
                     /locus_tag="ACHXWD_002767"
     CDS             10106..10675
                     /locus_tag="ACHXWD_002767"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR7621642.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10679..10906)
                     /gene="pptA"
                     /locus_tag="ACHXWD_002768"
     CDS             complement(10679..10906)
                     /gene="pptA"
                     /locus_tag="ACHXWD_002768"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR7621643.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11006..>11211)
                     /locus_tag="ACHXWD_002769"
     CDS             complement(11006..>11211)
                     /locus_tag="ACHXWD_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7621644.1"
                     /translation="RLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLR
                     RKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2607 a         2922 c         2993 g         2689 t
ORIGIN      
        1 taagactatc acttatttaa gtgatactgg ttgtctggag attcaggggg ccagtctagc
       61 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
      121 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      181 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      241 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      301 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      361 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      421 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      481 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      541 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      601 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      661 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      721 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      781 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      841 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      901 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      961 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
     1021 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1081 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1141 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1201 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1261 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1321 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1381 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1441 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1501 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1561 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1621 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1681 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1741 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1801 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1861 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1921 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1981 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     2041 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2101 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2161 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2221 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2281 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2341 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2401 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2461 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2521 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2581 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2641 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     2701 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2761 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2821 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2881 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2941 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     3001 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3061 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3121 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3181 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3241 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3301 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3361 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3421 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3481 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3541 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3601 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3661 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3721 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3781 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3841 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3901 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3961 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     4021 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4081 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4141 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4201 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4261 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4321 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4381 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4441 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4501 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4561 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4621 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4681 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4741 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4801 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4861 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4921 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4981 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     5041 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5101 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5161 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5221 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5281 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5341 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5401 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5461 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5521 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5581 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5641 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5701 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5761 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5821 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5881 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5941 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     6001 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6061 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6121 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6181 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6241 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6301 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6361 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6421 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6481 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6541 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6601 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6661 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6721 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6781 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6841 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6901 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6961 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     7021 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7081 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7141 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7201 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7261 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7321 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7381 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7441 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7501 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7561 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7621 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7681 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7741 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7801 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7861 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7921 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7981 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     8041 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8101 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8161 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8221 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8281 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8341 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8401 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8461 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8521 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8581 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8641 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8701 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8761 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8821 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8881 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8941 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     9001 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9061 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9121 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9181 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9241 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9301 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9361 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9421 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9481 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9541 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9601 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9661 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9721 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9781 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9841 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9901 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9961 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
    10021 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10081 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10141 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10201 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10261 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10321 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10381 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10441 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10501 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10561 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10621 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10681 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10741 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10801 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10861 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10921 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10981 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    11041 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11101 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11161 ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc c
//
LOCUS       DAZSBF010000132        11187 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZSBF010000132 DAZSBF010000000
VERSION     DAZSBF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.133"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..90
                     /locus_tag="ACHXWD_002770"
     CDS             <1..90
                     /locus_tag="ACHXWD_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR7621645.1"
                     /translation="TDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            69..173
                     /gene="speFL"
                     /locus_tag="ACHXWD_002771"
     CDS             69..173
                     /gene="speFL"
                     /locus_tag="ACHXWD_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR7621646.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            484..2682
                     /gene="speF"
                     /locus_tag="ACHXWD_002772"
     CDS             484..2682
                     /gene="speF"
                     /locus_tag="ACHXWD_002772"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR7621647.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2679..3998
                     /gene="potE"
                     /locus_tag="ACHXWD_002773"
     CDS             2679..3998
                     /gene="potE"
                     /locus_tag="ACHXWD_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR7621648.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4054..5694)
                     /gene="pgm"
                     /locus_tag="ACHXWD_002774"
     CDS             complement(4054..5694)
                     /gene="pgm"
                     /locus_tag="ACHXWD_002774"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR7621649.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5720..6265)
                     /gene="seqA"
                     /locus_tag="ACHXWD_002775"
     CDS             complement(5720..6265)
                     /gene="seqA"
                     /locus_tag="ACHXWD_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR7621650.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6450..7214
                     /gene="ybfF"
                     /locus_tag="ACHXWD_002776"
     CDS             6450..7214
                     /gene="ybfF"
                     /locus_tag="ACHXWD_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR7621651.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7354..7647
                     /gene="ybfE"
                     /locus_tag="ACHXWD_002777"
     CDS             7354..7647
                     /gene="ybfE"
                     /locus_tag="ACHXWD_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR7621652.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7787..8317
                     /gene="fldA"
                     /locus_tag="ACHXWD_002778"
     CDS             7787..8317
                     /gene="fldA"
                     /locus_tag="ACHXWD_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR7621653.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8527..8613
                     /gene="uof"
                     /locus_tag="ACHXWD_002779"
     CDS             8527..8613
                     /gene="uof"
                     /locus_tag="ACHXWD_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR7621654.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8606..9052
                     /gene="fur"
                     /locus_tag="ACHXWD_002780"
     CDS             8606..9052
                     /gene="fur"
                     /locus_tag="ACHXWD_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR7621655.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9137..9463)
                     /gene="chiQ"
                     /locus_tag="ACHXWD_002781"
     CDS             complement(9137..9463)
                     /gene="chiQ"
                     /locus_tag="ACHXWD_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR7621656.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9513..10919)
                     /gene="chiP"
                     /locus_tag="ACHXWD_002782"
                     /pseudo
     CDS             complement(9513..10919)
                     /gene="chiP"
                     /locus_tag="ACHXWD_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2724 a         2763 c         2799 g         2901 t
ORIGIN      
        1 acagattata gccatttcat ggatagaata actctacctt caactgacac agcaagaggt
       61 aaaggtaaat ggaaaataat aaccgcttaa tgcctcatat aaggcggaca acccatatca
      121 tgaagtttgc ccatcgtaat agttttgact ttcatttctt taatgcccgc tagtcttctg
      181 actaaagggc accccaacgt acaggtctcc ctgactttaa gcattacagg ttaatacctg
      241 tattcctcgg tgctcatata ctgctaaccc ttttaaactc taaataattc gagtcgcagc
      301 acttgcaact tgaggtatga cgagtatagc cagttaccgg gctggtctgg gttattgcat
      361 ctgcaaaaag caaactactg atttatttat cagcggtgga gctttgcttt ttttccggcg
      421 tgatcgattt ctcctttgag aaattgagga cctgctatta cctgaaataa agagatgaat
      481 aaaatgtcag aattaaaaat tgccgttagc cgttcttgcc cggattgttt ttccactcat
      541 cgtgcatgcg tgaatattga cgaaagtaat tatattgacg ttgccgccat tattttatca
      601 gtcaatgatg ttgaacgtgg aaaactcgat gaaatagacg ctactggcta tggcattcct
      661 gtttttattg caacggaaaa tgaagaacgt gtaccagcag aatatctgcc acgtatttct
      721 ggtgtcttcg agcattgcga atcacggaaa gagttttatg gacgtcagtt agaaaccgct
      781 gctagccatt atgaaacgca actgcgcccg cccttcttcc gcgcactggt cgattatgtc
      841 aatcagggca atagcgcttt tgactgcccg ggtcatcagg gaggtgagtt tttccgtcgt
      901 catccggcag ggaatcagtt cgtggagtac ttcggagaaa cgctgttccg ttccgactta
      961 tgcaacgcag acgtagcaat gggcgatctg ctgattcacg aaggcgcgcc atgcattgca
     1021 cagcaacatg cggcaaaagt gtttaatgcc gataaaacat acttcgttct aaatggcact
     1081 tcatcttcta acaaagtggt tttaaacgcc ctgctaacac cgggtgatct ggtgctgttt
     1141 gatcgcaata accacaaatc taaccaccac ggagcgttgc tacaggctgg tgcaacaccg
     1201 gtttatctgg aaacggcacg taacccgtat ggctttatcg gtggcattga tgcgcactgt
     1261 tttgaagaaa gttacctgcg tgagctgatc gcggaagtcg caccgcagcg ggcaaaagag
     1321 gctcgtcctt tccgcctcgc tgtgattcag ttaggcacct acgacggtac gatttataac
     1381 gcccgccagg tggtggataa aattggtcat ctgtgtgact acattctgtt tgactccgca
     1441 tgggttggct atgagcaatt tatcccgatg atggctgact gttcgccgct gttgctggat
     1501 cttaatgaga acgatccggg tattctggtt acgcaatccg tacataaaca gcaggctggt
     1561 ttttctcaaa cttcacaaat ccataaaaaa gacagccaca tcaaagggca acagcgttat
     1621 gtaccgcaca aacgcatgaa caacgccttt atgatgcacg cctccaccag cccgttctat
     1681 ccgctgtttg ccgcactgga tatcaacgcc aaaatgcatg aaggtgtcag cggtcgtaat
     1741 atgtggatgg attgtgtggt aaatggcatt aatgcccgca aactgatcct cgataactgt
     1801 cagcatattc gtccgttcgt acctgaactg gtggatggta aaccctggca gtcgtatgaa
     1861 acagcgcaaa ttgcggttga tctgcgcttc ttccagtttg taccagggga acactggcat
     1921 tcttttgaag gctatgcaga gaatcaatac tttgtcgatc cttgcaaact gttgctgaca
     1981 accccaggta ttgatgcacg taacggcgaa tatgaagcgt tcggtgtacc cgcgacgatt
     2041 cttgctaact tcctgcgcga aaatggcgta gtgccggaaa aatgcgatct taactccatc
     2101 ctcttcctgc tgactccggc agaagatatg gccaaacttc agcaacttgt tgccctgctg
     2161 gtacgcttcg aaaaactgct tgagtccgac gcgccattag cagaagtgct gccttccatc
     2221 tacaaacagc atgaagagcg ctacgccggt tataccctgc gtcagttgtg ccaggaaatg
     2281 catgatttgt atgcccgcca caacgtgaaa caactgcaaa aagagatgtt ccgcaaagcg
     2341 cacttcccac acgtcagcat gaatccgcaa gaagccaact acgcctattt acgcggtgaa
     2401 gtggagctgg ttcgtctgcc ggatgccgaa ggtcgtatcg ctgccgaagg ggcgcttcct
     2461 tatcctccgg gtgtgctgtg tgttgttccg ggtgaaatct ggggtggtgc tgttttgcgt
     2521 tacttcagcg ctctggaaga agggatcaac ctgctgccag gttttgcacc ggagctgcag
     2581 ggtgtctata tcgaagaaca tgatggtcgt aagcaagttt ggtgctatgt catcaagcct
     2641 cgtgatgcgc aaagcaccct gttgaaaggg gaaaaattat gagtcaggct aaatcgaaca
     2701 aaatgggcgt cgttcagtta accatactga cgatggtcaa catgatgggc tccggtatca
     2761 tcatgctgcc gacaaagctt gccgaagttg ggacaatttc aattatctcc tggctggtga
     2821 cagccgtagg ctcaatggca ctggcatggg cattcgcaaa atgcggtatg ttcagccgta
     2881 aatcaggtgg tatgggcggt tatgccgagt atgctttcgg taaatccggt aactttatgg
     2941 cgaactatac ctacggcgtc tcactgctga ttgctaacgt cgcgattgct atttcggcgg
     3001 ttggttacgg caccgaactg ctcggcgcga gtttgtcgcc agtgcagatt ggtcttgcga
     3061 ccatcggggt gctgtggatt tgtaccgtgg ctaactttgg tggtgcgcgc attaccgggc
     3121 aaatcagtag cattaccgtg tggggggtca ttattccggt ggtaggcctg tgcattatcg
     3181 gctggttctg gtttagcccg acgctttacg ttgattcctg gaatccgcat catgcaccgt
     3241 tcttcagtgc agtaggttct tccatcgcta tgacgctgtg ggcttttctc ggtctggagt
     3301 ctgcgtgtgc gaatactgat gtagtggaaa acccggaacg taatgtgcca atcgcggtac
     3361 tcggcggtac gttaggtgcg gcggtgattt atatcgtctc caccaacgtg attgcaggga
     3421 ttgtgcctaa tatggagctg gcaaattcaa cggcaccatt tggcctggcc ttcgcacaga
     3481 tgttcacacc agaagtgggt aaagtcatta tggcgctgat ggtgatgtcc tgctgcggtt
     3541 cgctgcttgg ctggcagttc accattgccc aggtgtttaa gtcttcatct gatgaaggct
     3601 acttccctaa aattttctcc cgtgtaacca aagtggatgc accggtgcag ggaatgttga
     3661 ccattgtgat tatccagagc gggttggcgc tgatgacgat tagcccgtcg ctaaacagtc
     3721 agttcaacgt gctggttaac ctggccgtgg tgaccaatat cattccgtat attctgtcaa
     3781 tggcggcatt agtcattatt cagaaggtcg ctaatgtgcc cccctcaaaa gcgaaagttg
     3841 caaactttgt tgcttttgtt ggcgcgatgt atagctttta tgcgctgtac tcatccgggg
     3901 aagaagccat gctgtacggt tccatcgtga ccttccttgg ttggacactg tatggtctgg
     3961 tatcaccacg ctttgaactg aaaaataaac acggttaaaa aagcaaagta aaaaagggcg
     4021 atcttgcgac cgcccttttt ttattaaatg tgtttacgcg tttttcagaa cttcgctaac
     4081 aatctcaacc gcttctttct caatctgctt gcgatgttct tcaccgagga agctttcgca
     4141 gtagatctta tatgcgtctt ccgtgcctga cggacgcgcg gcgaaccagc cgttgtcagt
     4201 catcactttc agaccgccga tagaagcacc attgcccggt gccgccgtca ggcgcgcggt
     4261 gatcgggtcg cctgccaggg tgctggcgct caccatttcc ggagacagct tagacagcgc
     4321 cgctttttgt gcggaagtcg cagctgcctg caaacggttg tagctcggcg caccaaagcg
     4381 ttttgccagt tcgttgtagt gttcctgcgg gttcttaccg gtgacagcgg tgatttccgc
     4441 cgccagcaga cacatgatga tgccgtcttt gtcggtggac cacggcgtgc cgtcgaaacg
     4501 caggaaggaa gcccctgcgc tctcttcgcc gccaaagccg aagctgccgt cgaacagacc
     4561 atcaacaaac catttgaaac ctaccgggac ttctaccagc ttgcggccca agtcattgac
     4621 cacacggtcg atcatcgcag aagaaaccag cgttttaccg acagcaacat ctttgcccca
     4681 ctgcggacga tgctggaaca ggtagttgat cgccaccgcc aggtagtggt tcggattcat
     4741 caaacctgcc ggagtgacga taccgtgacg gtcataatcc gggtcgttag caaacgccag
     4801 atcgaactta tcacgcaatg ccagcaggcc cgccatcgca cactcggagg agcagtccat
     4861 acggatcgcg ccgtctttat cgaggtgcat aaagcggaag gtttgatcga cctgatcgtt
     4921 aacgatggtc aggttgaggt tgtaatactc gccaattcgc ttccagtatt cgataccgga
     4981 accacccagc ggatcaacgc ccagcgtcag gcccgttttc tgaatcgccg ccatatcaac
     5041 gatatcggcc agaccttcca caaactgctg caccagatcc tgctctttca catgaccgga
     5101 tgccatcgct tcgtcgaggg agatacgctt cacgcctttc aggccatcgg ccatcagtgc
     5161 gttggcacga tcttccacca ctttagtgac gttggtatca gccgggccac catttggcgg
     5221 attgtacttg ataccaccat cttccggcgg gttatgggag ggtgtaatca cgataccgtc
     5281 tgccagcggg ccaccttttt tattatgaac caggatggca ttggaaacgg caggcgtcgg
     5341 agtgaagcca ttgttttcct gcacaatgac atcaacgccg ttcgctgcca gcacttccag
     5401 tacggaaatg aacgcaggct cggacagggc gtgagtatct ttacccacat agcaagggcc
     5461 agtgatgccg tttttcgcac gttcttcagc aattgcctga gcgatcgcca gaatgtgcgg
     5521 ctcgttaaag ctgtggcgcg ctgcactgcc acggtgaccg gaagtaccga atttcaccgc
     5581 gtgctccgca ttccctgctt ctggtttcag tacataatat tgcgccgtca gttgggcgac
     5641 gttaatcaaa tcactctgtt gtgcaggttg gcctgcacga ttgtggattg ccattgcttt
     5701 gtcctttgtc tgcaacgttt tagatagttc cgcaaacctt ctcaatcaat tccgccggga
     5761 attgcatcga ctgcatgatg tgttcgatca tgctgcattt acggccggtg ttggtgttgg
     5821 tgatcaccca atacggcgtg cctggcacat gtttcggctt ggtctgatta ccatttttca
     5881 gcagcgtttg ttcatctgcc gcaaagtaaa cgcgtgtacg accgtgcaac gattccgttg
     5941 cttcggcaaa cgcctgggcg tcaagagaat atagtgtaga caacagcagc ataaagcgat
     6001 tgaccgctcg cttttgctct gcgtattcgt cagaaagcag aagttcacgc attgcgcgaa
     6061 ccttgtcttt aatcgttttg accggcttcg cttcgacgat agcaggtgac gcaacgcgaa
     6121 cctctttcgt caccggagca gcaggctgtg atgcggcgga aaatttcaac atacgccgta
     6181 aaatgtcgga tgcgctctcg ccgatatgct tagtgtggct ggcaatatag ctgtagagtt
     6241 catcatcaac ttcaatcgtt ttcatcttaa tccagtacag tgtcttagct gaatacaagt
     6301 cgccaagagt atatgaataa atcccggcag cggatagcgt caagcctggc ttgcagaaaa
     6361 actcggaata aaccttattt gttgcagccc tgtggcacaa tggtcaacat aataccctaa
     6421 cccgacaacg cgaaaaaaag aactttgcca tgaaattgaa tatccgcgcg caaactgcac
     6481 aaaaccagca caataattct cccatcgttc ttgtccatgg tctgtttggt agcctcgaca
     6541 accttggcgt actggctcgc gatctggtaa acgatcacaa tatcatccag gttgatatgc
     6601 gtaaccacgg tctttcaccg agagatccgg taatgaatta cccggcgatg gcgcaggatc
     6661 ttgttgatac tctggatgca cagcagatcg acaaagcaac atttatcggt cactccatgg
     6721 gcggtaaagc ggtaatggca cttacggcac tggcccgcga tcgcatcgat aaactggtgg
     6781 cgatcgatat cgcaccggtc gactatcacg tacgccgtca tgatgagatt ttcgcggcta
     6841 tcaacgcggt cagtgaatcg gacgcacaaa ctcgccagca agcagcagca ataatgcacc
     6901 agcatcttaa tgaagaaggg gtgattcagt ttctgttgaa atcttttgtt gacggggagt
     6961 ggcgctttaa cgtgccggta ttgtgggatc agtatccgca tattgtgggt tgggagaaaa
     7021 tcccggcatg ggatcaccct gccctgttta tccctggcgg caattctccg tatgttagcg
     7081 agcagtaccg tgatgattta ctggcacaat ttccacaggc acgggcgcat gtgattgcag
     7141 gcgcgggtca ctgggtccat gctgaaaaac cggatgcggt attacgcgct atccgtcgct
     7201 atctcaatga ttaacgcaac tgattaaaaa cccagcgtcc gcgtcgcttt ccgcgcgtgg
     7261 gcgttggcgt gactcatcgc gctgatgtat tatggcgcgc tatccatccg ggcagaggcc
     7321 tggctgattg tttcccccga agtcaccaag atcatggcca aagaacaaac ggaccgtacg
     7381 acattagatc tgttcgcgca cgagcgtcga ccgggacgac cgaaaactaa tccgctttcg
     7441 cgcgatgaac agctgcgtat taataaacgc aaccagctaa aacgcgacaa agtacgtggc
     7501 cttaagcgtg tcgaactgaa gctgaacgcg gaagctgtcg aggcgctgaa cgagctggcg
     7561 gagtcgcgca atatgagccg tagcgaactg atcgaagaga tgttaatgca gcaactggcg
     7621 gcgctgcgta gtcagggcat cgtttaaatt tccactttca tgtagcacag tgtgcagtcc
     7681 tgctcgtttg ctgattccgc aagactgcct gttctgctat gattgccttt atccgtgggc
     7741 aattttccac ccccatttca ataagtttca agaggttatt tcactcatgg ctatcactgg
     7801 catctttttc ggcagcgaca ccggtaatac cgaaaatatc gcaaaaatga ttcaaaaaca
     7861 gcttggtaaa gacgttgccg atgtccatga cattgcaaaa agcagcaaag aagatctgga
     7921 agcttatgac attctgctgc tgggcatccc aacctggtat tatggcgaag cgcagtgtga
     7981 ctgggatgac ttcttcccga ctctcgaaga gattgatttc aacggcaaac tggttgcgct
     8041 gtttggttgt ggtgaccagg aagattacgc cgaatatttc tgcgacgcat tgggcaccat
     8101 ccgcgacatc attgaaccgc gcggtgcaac gatcgttggt cactggccaa ctgcgggcta
     8161 tcatttcgaa gcatcaaaag gtctggctga tgacgaccac tttgtcggtc tggctatcga
     8221 cgaagaccgt cagccggaac tgaccgctga acgtgtagaa aaatgggtta aacagatttc
     8281 tgaagagttg catctcgacg aaattctcaa tgcctgatgt gatgcggcgt agactcatgt
     8341 ctacgccgta ttaatagata atgccaatca aaataattgc tacaaatttg taacttttgc
     8401 tgttgtacct gtacaatgtc ccggtgttca agtggccttg ccgttgtaaa tgtaagctgt
     8461 gccacgtttt tattaacaat atttgccagg gacttgtggt tttcatttag gcgtggcaat
     8521 tctataatga tacgcattat ctcaagagca aattctgtca cttcttctaa tgaagtgaac
     8581 cgcttagtaa caggacagat tccgcatgac tgataacaat accgccctaa agaaagctgg
     8641 cctgaaagta acgcttcctc gtttaaaaat cctagaagtt cttcaggagc cggacaacca
     8701 tcacgtcagt gcggaagatt tatacaaacg tctgatcgat atgggtgaag aaattggtct
     8761 ggctacggta tatcgcgtac tgaaccagtt tgacgacgct ggtatcgtca cccgccacaa
     8821 ttttgaaggc ggtaaatccg tatttgaact gacacagcaa catcaccacg atcacctgat
     8881 ctgcctcgac tgcggcaagg ttatcgaatt tagtgatgat tccatcgaag cgcgtcagcg
     8941 tgaaattgcc gcaaaacatg gcattcgcct gactaaccac agtctctatc tttacggtca
     9001 ctgtgccgaa ggcgattgcc gcgaagatga acatgcgcac gaaggcaaat aagccagcct
     9061 gaatgagaaa aagccaacct gcgggttggc ttttttatgc aagggaaata agaaaagcga
     9121 gagttacagc tctcacttat ttgttattac tgcgaatttc ctgccaaact ttatcacaat
     9181 cggctttcac cgcctgatca tttccggttt gcgttgcacg cagatactgc tgataatcca
     9241 gtacgcggac actttcctgg tcggcaaaat gctgatgttg tttctctttc ttcagcacgt
     9301 ttaacacgct ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg
     9361 cgctgtatgc ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta
     9421 ccagccccga tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg
     9481 tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc
     9541 ataaatttca cgtcacgctc atcctggaag atgttgccgt aaccaccgcc ccagcttggg
     9601 atgtcggagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga
     9661 ccgtcctgaa tggtgtagac cgcatccagg ctgtatgcag actcttcaat agtccggttt
     9721 ttgtcgtagt acgcatccgg attgctctgc taggtcgcag gtttagcatc ccatgcgtaa
     9781 acgtaggaag cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca
     9841 ccgaagaaga ccgctttttc gccgttagcg ttgaagtcgg aacggttatc ccaccagata
     9901 tccaggcggc cgttggagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc
     9961 tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg
    10021 taaccaaagg tcaacgcttg cagccaggcg gtgccgtcat aaaggtcgtt gacgctgcga
    10081 tcgtcaactt tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg
    10141 atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca
    10201 aatgccgctt ccagtacgaa gttatttttg aagtcgtatt tcgccccaag ggagtgcaga
    10261 taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc
    10321 gctttgtatt cgttggtcca catgtaggag aaactcaacg caccagcatc gccgtaatca
    10381 aaattcgccc cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct
    10441 aacagcgttt gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta
    10501 aatttggccg cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca
    10561 taggctttat tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc
    10621 gccatttcaa tcgccgtaaa cgcagcaata tcaaggccga acatatcagc agcataaccg
    10681 gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg
    10741 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag
    10801 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
    10861 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
    10921 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa
    10981 ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac
    11041 gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt
    11101 tgatgatttt tgttacaagg cttaagtttg cggttgaaag gcttgcagaa gaataaaagc
    11161 gtgaaattcc agcaagatga aagcggt
//
LOCUS       DAZSBF010000133        11151 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZSBF010000133 DAZSBF010000000
VERSION     DAZSBF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.134"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHXWD_002783"
     CDS             53..352
                     /locus_tag="ACHXWD_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR7621657.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHXWD_002784"
     CDS             complement(472..651)
                     /locus_tag="ACHXWD_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR7621658.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHXWD_002785"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHXWD_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR7621659.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHXWD_002786"
     CDS             2090..2668
                     /locus_tag="ACHXWD_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR7621660.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHXWD_002787"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHXWD_002787"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR7621661.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHXWD_002788"
     CDS             4347..5945
                     /locus_tag="ACHXWD_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR7621662.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHXWD_002789"
     CDS             complement(5949..7499)
                     /gene="yoaE"
                     /locus_tag="ACHXWD_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR7621663.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHXWD_002790"
     CDS             complement(7487..7645)
                     /gene="yoaL"
                     /locus_tag="ACHXWD_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR7621664.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7962..8933
                     /gene="manX"
                     /locus_tag="ACHXWD_002791"
     CDS             7962..8933
                     /gene="manX"
                     /locus_tag="ACHXWD_002791"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR7621665.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8996..9796
                     /gene="manY"
                     /locus_tag="ACHXWD_002792"
     CDS             8996..9796
                     /gene="manY"
                     /locus_tag="ACHXWD_002792"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR7621666.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9809..10660
                     /gene="manZ"
                     /locus_tag="ACHXWD_002793"
     CDS             9809..10660
                     /gene="manZ"
                     /locus_tag="ACHXWD_002793"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR7621667.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10715..>11151
                     /locus_tag="ACHXWD_002794"
     CDS             10715..>11151
                     /locus_tag="ACHXWD_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR7621668.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIY"
BASE COUNT         2737 a         2813 c         2820 g         2781 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggg ttatcggcat cgtgctgaac attggcctga
     6721 cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag cggcaaagtc
     6781 gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat gatgatcgag
     6841 aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc ttctgccacc
     6901 agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt ggggtgctgg
     6961 ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc aatcactacc
     7021 gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac cgcatccaac
     7081 gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact ggcgtagcct
     7141 ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc ggttgttgct
     7201 ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga gaaggagaaa
     7261 tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag cgacagcagc
     7321 cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc gcgttgtttt
     7381 ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc cagcacaatt
     7441 tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa gaattccatg
     7501 acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc gtaacgacaa
     7561 aatgcaaacg aaaggtgcgg catagagtgc cagaaaggca ggcgttaaaa ggcctgatgc
     7621 tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc atggttgtta
     7681 aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc ttcctttgca
     7741 aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa gttaaattac
     7801 ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga aacgcagggg
     7861 tttttggttg tagcccttat ctgaatcgat tcgattgtgg acgacgattc aaaaatacat
     7921 ctggcacgtt gaggtgttaa cgataataaa ggaggtagca agtgaccatt gctattgtta
     7981 taggcacaca tggttgggct gcagagcagt tgcttaaaac ggcagaaatg ctgttaggcg
     8041 agcaggaaaa cgtcggctgg atcgatttcg ttccaggtga aaatgccgaa acgctgattg
     8101 aaaagtacaa cgctcagttg gcaaaactcg acaccactaa aggcgtgctg tttctcgttg
     8161 atacatgggg aggcagcccg ttcaatgctg ccagccgcat tgtcgtcgac aaagagcatt
     8221 atgaagtcat tgcaggcgtt aacattccaa tgctcgtgga aacgttaatg gctcgtgatg
     8281 atgacccaag ctttgatgaa ctggttgcgc tggcagtaga aacaggccgt gaaggcgtga
     8341 aagcactgaa agccaaaccg gttgaaaaag ccgcgccagc acccgctgcc gcagcaccaa
     8401 aagcggctcc aactccggca aaaccaatgg ggccaaacga ctacatggtt attggccttg
     8461 cgcgtatcga cgaccgtctg attcacggtc aggtcgccac ccgctggacc aaagaaacca
     8521 atgtctcccg tattattgtt gttagtgatg aagtggctgc ggataccgtt cgtaagacac
     8581 tgctcaccca ggttgcacct ccgggcgtaa cagcacacgt agttgatgtt gccaaaatga
     8641 ttcgcgtcta caacaacccg aaatatgctg gcgaacgcgt aatgctgtta tttaccaacc
     8701 caacagatgt agagcgtctc gttgaaggcg gcgtgaaaat cacctctgtt aacgtcggtg
     8761 gtatggcatt ccgtcagggt aaaacccagg tgaataacgc ggtttcggtt gatgaaaaag
     8821 atatcgaggc gttcaagaaa ctgaatgcgc gcggtattga gctggaagtc cgtaaggttt
     8881 ccaccgatcc gaaactgaaa atgatggatc tgatcagcaa aatcgataag taacgtattg
     8941 tgttgattat cactcagttt tcacacttaa gtcttacgta aacaggagaa gtacaatgga
     9001 gattaccact cttcaaattg tgctggtatt tatcgtagcc tgtatcgcag gtatgggatc
     9061 aatcctcgat gaatttcagt ttcaccgtcc gctaatcgcg tgtaccctgg tgggtatcgt
     9121 tcttggggat atgaaaaccg gtattattat cggtggtacg ctggaaatga tcgcgctggg
     9181 ctggatgaac atcggtgctg cagttgcgcc tgacgccgct ctggcttcta tcatttctac
     9241 cattctggtt atcgcaggtc atcagagcat tggtgcaggt atcgcactgg caatccctct
     9301 ggccgctgcg ggccaggtac tgaccatcat cgttcgtact attaccgttg ctttccagca
     9361 cgctgcggat aaggctgctg ataacggcaa cctgacagcg atttcctgga tccacgtttc
     9421 ttctctgttc ctgcaagcaa tgcgtgtggc tattccggcc gtcatcgttg cgctgtctgt
     9481 tggtaccagc gaagtacaga acatgctgaa tgcgattccg gaagtggtga ccaatggtct
     9541 gaatatcgcc ggtggcatga tcgtggtggt tggttatgcg atggttatca acatgatgcg
     9601 tgctggctac ctgatgccgt tcttctacct cggcttcgta accgcagcat tcaccaactt
     9661 taacctggtt gctctgggtg tgattggtac tgttatggca gtgctctaca tccaacttag
     9721 cccgaaatac aaccgcgtag ccggtgcgcc tgctcaggca gctggtaaca acgatctcga
     9781 taacgaactg gactaacagg tgagcgaaat ggttgataca actcaaacta ccaccgagaa
     9841 aaaactcact caaagtgata ttcgtggcgt cttcctgcgt tctaacctct tccagggttc
     9901 atggaacttc gaacgtatgc aggcactggg tttctgcttc tctatggtac cggcaattcg
     9961 tcgcctctac cctgagaaca acgaagctcg taaacaagct attcgccgtc acctggagtt
    10021 ctttaacacc cagccgttcg tggctgcgcc gattctcggc gtaaccctgg cgctggaaga
    10081 acagcgtgct aatggcgcag agatcgacga cggtgctatc aacggtatca aagtcggttt
    10141 gatggggccg ctggctggtg taggcgaccc gatcttctgg ggaaccgtac gtccggtatt
    10201 tgcagcactg ggtgccggta tcgcgatgag cggcagcctg ttaggtccgc tgctgttctt
    10261 catcctgttt aacctggtgc gtctggcaac ccgttactac ggcgtagcgt atggttactc
    10321 caaaggtatc gatatcgtta aagatatggg tggtggcttc ctgcaaaaac tgacggaagg
    10381 ggcgtctatc ctcggcctgt ttgtcatggg ggcattggtt aacaagtgga cacatgtcaa
    10441 catcccgctg gttgtctctc gcattactga ccagacgggc aaagaacacg ttactactgt
    10501 ccagactatt ctggaccagt taatgccagg cctggtacca ctgctgctga cctttgcttg
    10561 tatgtggcta ctgcgcaaaa aagttaaccc gctgtggatc atcgttggct tcttcgtcat
    10621 cggtatcgct ggttacgctt gcggcctgct gggactgtaa gactgttgta cactaccggg
    10681 gccttttggc cccgtttttt tatctggagg attaatgaca atcacggacc tggtactgat
    10741 tcttttcatc gccgcactcc tggccttcgc gatctacgat cagttcatca tgccccgccg
    10801 taacggcccc accctgctgg caattccttt gctccggcgt ggtcgcatcg atagcgttat
    10861 cttcgtcgga ttgattgtca ttcttatcta taacaacgtc acgaatcatg gtgcgttaat
    10921 aacgacatgg ttattaagcg cactggctct gatgggtttt tatatattct ggatccgcgt
    10981 tccgaagatc atctttaaac aaaaaggttt tttcttcgcc aatgtctgga ttgaatatag
    11041 ccgaatcaaa gcgatgaact tgtcggaaga tggcgtgttg gtgatgcaat tagaacagcg
    11101 tcggctgtta atccgcgttc gaaatatcga cgatctggaa aaaatttata a
//
LOCUS       DAZSBF010000134        11067 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZSBF010000134 DAZSBF010000000
VERSION     DAZSBF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.135"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            182..1564
                     /gene="dosC"
                     /locus_tag="ACHXWD_002795"
     CDS             182..1564
                     /gene="dosC"
                     /locus_tag="ACHXWD_002795"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR7621669.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1589..3988
                     /gene="dosP"
                     /locus_tag="ACHXWD_002796"
                     /gene_synonym="pdeO"
     CDS             1589..3988
                     /gene="dosP"
                     /locus_tag="ACHXWD_002796"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR7621670.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4247..4828
                     /gene="ddpX"
                     /locus_tag="ACHXWD_002797"
     CDS             4247..4828
                     /gene="ddpX"
                     /locus_tag="ACHXWD_002797"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR7621671.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4842..6392
                     /locus_tag="ACHXWD_002798"
     CDS             4842..6392
                     /locus_tag="ACHXWD_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR7621672.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6394..7416
                     /locus_tag="ACHXWD_002799"
     CDS             6394..7416
                     /locus_tag="ACHXWD_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7621673.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7413..8309
                     /gene="ddpC"
                     /locus_tag="ACHXWD_002800"
     CDS             7413..8309
                     /gene="ddpC"
                     /locus_tag="ACHXWD_002800"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR7621674.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8306..9292
                     /locus_tag="ACHXWD_002801"
     CDS             8306..9292
                     /locus_tag="ACHXWD_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7621675.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            9321..10211
                     /locus_tag="ACHXWD_002802"
     CDS             9321..10211
                     /locus_tag="ACHXWD_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR7621676.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(10267..10698)
                     /gene="osmC"
                     /locus_tag="ACHXWD_002803"
     CDS             complement(10267..10698)
                     /gene="osmC"
                     /locus_tag="ACHXWD_002803"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR7621677.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2750 a         2733 c         2825 g         2759 t
ORIGIN      
        1 gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat tgaaactgtc
       61 ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg gtgcagttag
      121 tcaatctcgc acgttgcgtt ttgattccgt ctgatttccc agccttataa gggtgtgaat
      181 tatggagatg tattttaaaa gaatgaaaga tgagtggacc ggacttgtcg aacaggcaga
      241 tccactcatt cgtgctaaag ccgcggaaat tgccgttgcg catgctcatt atctgagtat
      301 cgagttttat cgaattgtcc gcatcgaccc gcatgccgaa gaattcttga gtaatgaaca
      361 agttgagcgg cagttgaaaa gtgcgatgga acgctggatt attaacgtgc tttctgccca
      421 ggttgacgat gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag tgcatgcccg
      481 cataggaatt ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa aaatcctcta
      541 tccggtaatc ttctcttcgg attattccgc cgcagaaaaa cttcaggtct atcatttctc
      601 gattaacagt attgatatcg cgatggaagt gatgacccgc gcgtttacct ttagtgacag
      661 tagtgcctca aaggaagatg aaaattatcg tatcttctcg ttactggaaa acgccgaaga
      721 agaaaaagaa cggcaaatag cctcaatact ttcatgggaa atagatatta tctataaaat
      781 cctgctggat tctgatttag gcagtagttt gccattaagc caggctgatt ttggcctgtg
      841 gtttaaccat aaaggtcgac attattttag tggcattgcc gaagtaggcc atatctcccg
      901 tctcattcag gatttcgacg gtattttcaa tcaaaccatg cgtaacacca ggaatttgaa
      961 taacagaagt ctgcgggtga aatttttatt acagataaga aataccgtat cgcagattat
     1021 taccttgctg cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta tggatgtact
     1081 aacgaaatta cttaaccgcc gtttcctgcc gactatcttc aaacgcgaaa ttgcccatgc
     1141 caaccggacc ggtacaccgc tgtcagtgct gattattgac gttgataaat tcaaagagat
     1201 caacgatacg tggggccata acactggcga tgaaattctg cgtaaagtct ctcaggcctt
     1261 ttatgacaac gtccacagta gtgattatgt ttttcgctac gggggcgatg aatttatcat
     1321 tgttttgact gaagcttctg aaaacgaaac gttacgtacc gcagaacgta ttcgcagtcg
     1381 ggtggagaaa accaaactga aagccgcaaa cggcgaggat attgccctct cactttccat
     1441 cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa tagccgatga
     1501 agctctgtat atcgccaaaa gacgaggtag aaaccgtgtt gaactctgga aagccagtct
     1561 ttagatgcgc caggatgcag aggtaatcat gaagctaacc gatgcggaaa atgccgccga
     1621 tggcattttt ttccccgccc ttgaacaaaa tatgatgggc gcggtgttaa ttaacgaaaa
     1681 tgatgaagtg atgtttttca accccgccgc agagaagctc tgggggtaca aacgtgaaga
     1741 agtcattggc aataacattg atatgctgat tccacgggat ttgcgtcctg cgcatcctga
     1801 atacattcgt cataaccgtg aaggtggtaa agcgcgcgtc gaggggatga gtcgggagct
     1861 gcagctggag aaaaaagacg gcagtaaaat ctggacccgt tttgcgctat cgaaagtgag
     1921 cgccgagggg aaagtttatt acctggcgct ggtacgggat gccagcgtag aaatggcgca
     1981 aaaagaacag acccgacaat tgattattgc cgttgaccat ctcgaccgac cggtgattgt
     2041 cctcgatccg gaacgccata ttgtgcagtg caatcgcgca tttaccgaaa tgtttggtta
     2101 ctgcattagc gaagccagcg gtatgcagcc cgatacactc ctgaacattc ctgaattccc
     2161 tgccgataac cgcattcgtt tacaacagtt gctatggaaa accgcccgcg atcaggacga
     2221 atttctgctg ttgacacgca ccggtgaaaa aatctggatt aaagcctcta tcagcccggt
     2281 ttatgacgtg ctcgcgcatc tgcagaacct ggtaatgacc ttctcggata tcaccgaaga
     2341 acggcagatc cgccagcttg aaggcaatat tctcgccgcc atgtgcagca gcccgccatt
     2401 tcatgaaatg ggggaaatca tttgtcgtaa catcgaatct gttctcaacg aatcgcatgt
     2461 ttcgctgttc gcactgcgca acgggatgcc gatacactgg gcgtcatctt cgcacggtgc
     2521 agaagttcaa aatgcgcaaa gctggtcagc gaccattcgt cagcgtgatg gtgcgcctgc
     2581 ggggatcctg caaattaaaa cctcgtcagg agcagaaacc agcgccttta tcgaacgcgt
     2641 ggcagatatc agccagcata tggccgcgct ggcgctggaa caggaaaaaa gccgtcagca
     2701 tattgaacaa ctcatccaat ttgatccgat gaccggtctg ccaaatcgca ataacctgca
     2761 caattacctc gatgaccttg tcgacaaagc cgtctctccg gtggtgtatc tcatcggtgt
     2821 tgaccatatt caggatgtga ttgatagcct tggctatgcg tgggccgatc aggcattgct
     2881 ggaagtggtc aatcgctttc gtgaaaaact caaaccggat cagtatctct gtcgtatcga
     2941 aggtacgcag tttgtcctcg tgagcctgga aaacgacgtc agtaacatta cccaaatcgc
     3001 cgatgagcta cggaatgtgg tcagcaagcc gataatgatt gacgataaac cattcccgct
     3061 aaccttgagt attggcatca gctacgacgt gggtaaaaac cgcgattacc tgctctccac
     3121 tgctcacaat gcaatggatt atattcgcaa gaatggcggt aacggctggc agttcttcag
     3181 cccggcgatg aacgaaatgg taaaagagcg tttggtttta ggcgcagcgc tgaaagaagc
     3241 gattagcaat aaccaactga aactggttta ccagccgcaa atcttcgcag aaacgggtga
     3301 actgtacggc atcgaagccc ttgctcgctg gcacgatccc cagcatggtc atgtaccccc
     3361 ttcacggttt attcctctcg cagaagagat tggtgaaatc gaaaatattg ggcgctgggt
     3421 catcgcggaa gcttgccgtc agttagcaga atggcgtagc cagaatattc atatcccggc
     3481 gttatccgtg aacttgtcgg cgctgcactt tcgcagtaat cagctgccta atcaggtgtc
     3541 tgatgcaatg caagcctggg gtattgacgg ccaccagctg acggtagaaa tcacggaaag
     3601 catgatgatg gaacacgata ccgaaatctt taagcgcatt cagatcctgc gcgatatggg
     3661 cgtgggctta tcggtagatg attttggcac gggcttttcc ggattatccc gcttagtcag
     3721 tcttccggta acggaaatca aaattgacaa aagttttgtc gatcgttgtc tgacggaaaa
     3781 acgcatcctt gccttacttg aagccattac cagcattggg caaagcctca atttaaccgt
     3841 cgtggcggaa ggcgtcgaaa ccaaagagca atttgagatg ctacgcaaga tccactgtcg
     3901 cgttattcag ggatatttct tttcccgccc cctacccgcc gaggaaattc caggctggat
     3961 gagcagcgtg ttaccgctga aaatctgaca aattcctctc gcccgcactc gcgggttttc
     4021 atttaacgtg acgctgtcac ttaaatactg tgatttcagc tacgattccg ggagattcct
     4081 tccttaactt cccctcttgc accaaaactg cactacgctg cacaaatgtt gatcaaaagt
     4141 taaaaaatat aactttaagc cagcattctg gcgcttatcc cggcatggca tgagatctgc
     4201 ataagcggaa agcgcagcaa tttttgtctt aatacaggaa ccgtttatgt cggataccac
     4261 cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga aatacgcctg
     4321 cgctgataac atcacaggta aagctattta ccagcaagcg cgttgtctgt tacacaagga
     4381 tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct tacaactggt
     4441 gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag cctgcccaga
     4501 cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca cggcgatcga
     4561 cctgacgctt cgtgatgagc acgggaacat cctggatatg ggcgcagggt tcgatgaaat
     4621 gcacgagcgc tcccatgcct atcacccttc cgtcccgccc gccgctcagc gcaatcgcct
     4681 gttgctgaat gcgataatga ctggtggcgg ttttgtcggt atctccagcg aatggtggca
     4741 cttcgaatta ccccaggcag cgagttaccc tctgctcgct gatcaattct cttgttttat
     4801 atcccctggc acacagcacg tcagttaatc aggaaactgt catgaagaga tcgatatcgt
     4861 ttcgtcccac attgctcgcg ctcgtccttg ccacaacttt cccggttgcg cacgccgccg
     4921 taccgaaaga tatgctggtg attggtaagg ccgccgatcc acaaaccctc gacccggcgg
     4981 taacaataga taataacgac tggacagtga cctacccgtc ttatcagcgg ctggttcagt
     5041 acaaaacgga cggtgataaa ggctcaaccg acgttgaagg cgatctggca agtagctgga
     5101 aagcgtctga cgatcaaaaa gagtggacgt tcaccctgaa ggacaatgct aaatttgccg
     5161 atggcacacc tgtcactgcc gaagcagtaa aactctcttt tgagcggtta ctaaaaatcg
     5221 gccaggggcc agcagaagca tttcccaaag atttaaagat tgatactccc gacgaacata
     5281 cggtgaagtt tacccttagc caaccattcg caccgttcct ctacacgcta gcgaatgacg
     5341 gtgcatccat tatcaatccg gcggtcttaa aggaacatgc agcggatgat gctcgcggct
     5401 tcctcgcgca aaataccgcc ggttccggac catttatgct gaaaagcggg caaaaaggtc
     5461 agcaattagt tctggtgcca aatccgcatt accccggcaa taaaccgaac tttaagcgag
     5521 tatcggtaaa aattatcggt gaaagtgcct cccgtcgcct gcagctctcc cgtggcgaca
     5581 ttgacattgc cgatgcgctg ccggtggatc aactcaacgc cctgaagcag gaaaataaag
     5641 tcaatgtggc agagtatccg tcactgcgcg ttacctatct gtatctgaat aacagcaaag
     5701 cgcctcttaa tcaggcggat ctgcgtcggg ccatttcctg gtctaccgat tatcagggca
     5761 tggttaacgg cattctgagt ggtaacggaa aacagatgcg cggcccgatt ccggaaggca
     5821 tgtggggcta cgatgcgacg gcaatgcaat acaaccatga cgaaacgaaa gccaaagccg
     5881 aatgggataa agtgacgagc aaacccacca gcctgacgtt tctctactcc gataacgatc
     5941 cgaactggga gcctattgct ctggcgacac aatccagtct caacaagctg ggcatcaatg
     6001 tgaagctgga aaagctggcg aacgccacca tgcgcgacag agtgggtaaa ggtgattacg
     6061 acattgcgat tggcaactgg agtccggatt ttgccgaccc gtatatgttt atgaattact
     6121 ggtttgagtc agacaaaaaa ggtctgccgg gtaaccgctc gttctatgaa aacagtgagg
     6181 tcgataagtt actgcgcaat gcgcttgcga ccaccgacca gacgcagcgt acccgggact
     6241 accagcaggc acagaaaatc gtcattgatg acgctgctta tgtgtacctg ttccagaaaa
     6301 actaccaact ggcgatgaac aaagaggtga aaggctttgt gttcaatccc atgctggaac
     6361 aggtcttcaa tatcaatacc atgagtaaat aacgtgacct tctggagtat tttacgccaa
     6421 cgctgctggg ggctggtgct cgtggtggcg ggcgtctgcg tgattacgtt tattatctcg
     6481 catctgatcc ctggcgatcc ggcgcggtta ctggcgggtg accgcgccag cgatgctatc
     6541 gtggaaaata ttcgccagca actgggactg gaccagccac tgtatgtaca gttttaccgc
     6601 tacttcagcg acctgtttca tggtgacctg ggaacatcca ttcgtaccgg gcgtccggtg
     6661 ctggaagagt tgcgtatatt tttcccggcg acgctggaac tggctttttg tgccctgctg
     6721 ctggcactcc tgattggcat cccgctgggc atactctctg cagtctggcg aaatcgctgg
     6781 ctggatcatc tggtgcgaat aatggccatt accggaatct ccacacctgc gttctggctt
     6841 ggactgggcg tcattgtgct gttttatggt catctgcaaa ttcttcccgg cggcggaagg
     6901 cttgatgact ggctggatcc accaacgcac gttaccggct tttatctgct cgatgcgctg
     6961 cttgaaggca acggtgaagt cttcttcaat gcgttgcaac atctcatctt accggcatta
     7021 acgctggcgt tcgttcacct gggaattgtc gctcgccaga tccgctcagc gatgctggaa
     7081 caattgagtg aagactacat tcgtaccgcc cgggccagcg gcttgcccgg ctggtatatc
     7141 gttttatgtt atgcgctacc caatgcgttg atcccatcga ttaccgtatt gggtctggcg
     7201 ctgggcgatt tgttgtatgg cgcagtgctc accgaaaccg tttttgcctg gcccggaatg
     7261 ggtgcatggg tagtaacatc aatacaggcg ctcgacttcc cggcagtgat gggctttgcc
     7321 gtcgtggttt catttgctta tgtgctggtc aacctggtgg tggatttgct ctatttgtgg
     7381 attgatccgc gtatcggacg tggaggtggt gaatgatgct aagcgaggaa acgtccgccg
     7441 tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg ctgaaaggca
     7501 gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gctattgatg atgatttttt
     7561 caccgtggct tgcgacgcat gatcccaacg ccattgattt aaccgcccgc cttttgccgc
     7621 cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttc agccgcgtac
     7681 tggtcggcag tcagcaatca attctcgccg gattagtcgt ggttgccatt gcgggtatga
     7741 ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac gccatcatca
     7801 tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg gcactggctg
     7861 ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg cgaattccct
     7921 tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc tatgttcagg
     7981 cggcgaaaac ctttggtgcg tcgcgttggc atctgatcag ctggcatatt ttacgtaact
     8041 ccctgccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt ttaatggccg
     8101 ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg ggcgcgatgg
     8161 tagctaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt ccgggggcag
     8221 cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc gatctgcttg
     8281 acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc aacagctgca
     8341 tttgagtttc cccggtttta acggttatgt tcacgtgctc aacaatgtgt ccttgcagat
     8401 taaccgcggt gaaattgtcg gtctggtggg agaatccggc tcaggtaaat cagtcaccgc
     8461 aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg ggcagatttc
     8521 actgctggga gacgatgttc ttaacgctcg ggagaaacag cttcgtcagt ggcgcggcgc
     8581 acgagtggcg atgatctttc aggaaccgat gaccgccctc aatccgacac gtcgaatagg
     8641 tcttcagatg atggacgtga tccgccatca tcaaccaata agtcgtcggg aagccagagc
     8701 taaagcgatt accctgctgg aagagatgca aatcccggat gccgtggaag ttatgtcgcg
     8761 ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc tggcattctc
     8821 ctgcgagccg caattgatta ttgccgacga accgactacg gcgctggacg tcacggtaca
     8881 gttgcaggta ctgcgtttgc ttaaacataa agctcgcgcc agtggaactg cggtactgtt
     8941 catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg tgatgtatgc
     9001 cggaagcgta attgaaagcg gcgtgacggc agacgttatc catcatcccc ggcatccgta
     9061 taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc cattacccgc
     9121 cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt ttcgcgatcg
     9181 ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt gtggtgacaa
     9241 caaccagcgc tgcgcctgtt ggtatccgca gcaggaggtc ataagtatct gacacgttat
     9301 taacgttacg cgacgtccat atcaatttcc cggcccgtaa aagctggctt ggtaaaacta
     9361 cggaacatgt tcatgcgatt aatggtattg atttacagat ccgccgtggt gaaaccttag
     9421 ggatcgtcgg tgagtcaggc tgcggcaaaa gcaccctcgc acagctttta atgggtatgc
     9481 tgcaaccgag ccacgggcaa tacatccgtt caggatcaca acgcattatg cagatggtgt
     9541 ttcaggaccc gctctcttcg cttaatccgc gcttatcggt gtggcgcatc atcacagaac
     9601 cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca aaggagttgg
     9661 ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg ttctccggcg
     9721 ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac gtgattgtgc
     9781 ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc aatttactgg
     9841 taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat gtctcggtga
     9901 tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta gaactgggcg
     9961 acgcgcagca ggtgctgacg gcacctgcac atccatacac ccgattattg ctggattccc
    10021 tccccgccat tgataaaccg ctggaggaag aatgggcatt atgtaaaacg gatctgccgg
    10081 gaaaccgcac gttgccacaa ggctgttttt tctacgaacg ttgccctctg gcaacccacg
    10141 gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc cggtgctggc
    10201 gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc gtcatccggc
    10261 aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagaaac
    10321 cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat caatacccgg
    10381 caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaatcggcat ccactttatc
    10441 cagcgacaca tcggcggtgg tatcaatcga tgttggcgtg aatcccgctt cccccagcat
    10501 taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt cttcagggtt
    10561 ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt tcagcacgcc
    10621 actctgggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt gtgcctgacc
    10681 tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt atagactacg
    10741 tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa aatattactt
    10801 ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca agagatattg
    10861 catcaatatc aatcaactca ccacaataat aatacatatc aatatcatgg agttaaaata
    10921 taaaaatcat caaccaggac taatcttaac aacgaagcgg caaatatttg ccatgctgaa
    10981 tgtgctttat aaatctgcga tccgtagcag acaccataaa tacacagaca cggagaatca
    11041 ctatgtttac ttattatcag gcagaaa
//
LOCUS       DAZSBF010000135        11023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZSBF010000135 DAZSBF010000000
VERSION     DAZSBF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.136"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..208
                     /locus_tag="ACHXWD_002804"
     CDS             <1..208
                     /locus_tag="ACHXWD_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR7621678.1"
                     /translation="IVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFA
                     QTTKGVLETGIYRGVVSIYLSQDI"
     gene            300..2984
                     /locus_tag="ACHXWD_002805"
     CDS             300..2984
                     /locus_tag="ACHXWD_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR7621679.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2981..4069
                     /locus_tag="ACHXWD_002806"
     CDS             2981..4069
                     /locus_tag="ACHXWD_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR7621680.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHXWD_002807"
                     /pseudo
     CDS             complement(4152..5449)
                     /gene="exuT"
                     /locus_tag="ACHXWD_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHXWD_002808"
                     /pseudo
     CDS             5931..7343
                     /gene="uxaC"
                     /locus_tag="ACHXWD_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
     gene            7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHXWD_002809"
     CDS             7358..8845
                     /gene="uxaA"
                     /locus_tag="ACHXWD_002809"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR7621681.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8928..9479
                     /locus_tag="ACHXWD_002810"
     CDS             8928..9479
                     /locus_tag="ACHXWD_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR7621682.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHXWD_002811"
     CDS             complement(9484..10728)
                     /gene="sstT"
                     /locus_tag="ACHXWD_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR7621683.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2915 a         2672 c         2856 g         2580 t
ORIGIN      
        1 gattgttccg ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac
       61 ttttaccgca accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa
      121 cctgatgttt gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt
      181 aagcatttat ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg
      241 aggggtagcc ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca
      301 tggataaaaa attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat
      361 taaccgaaat tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg
      421 tttatttttc agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa
      481 catttcttga tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa
      541 tcgcgcatat agtgaaagaa aaattatcgc agccgctagc tcaccacggt gaattggctt
      601 gcggttatgt acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa
      661 tctataatga tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt
      721 tcgatgcgaa gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc
      781 agcaagacat caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg
      841 gtgcgctggg aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg
      901 atgcagatga tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt
      961 ttttacgtcg ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac
     1021 aaggcgggaa ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg
     1081 ggtcgacact gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc
     1141 tgctttcgcg caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt
     1201 atctcaatag tggttcgcaa tttattgata ccagttcctt tccgccaggc agctacagcg
     1261 tggcattaaa agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca
     1321 aaaccggcgg tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat
     1381 cacaggtttc tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc
     1441 cgcaatatga gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag
     1501 gtaataactg gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt
     1561 tccgtaacga tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat
     1621 ggccgacgtt gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca
     1681 gagagagcta taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt
     1741 ttgtcggctg gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt
     1801 tgcgttggga taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa
     1861 gtatctctga aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt
     1921 tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg
     1981 gcttgtactt atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc
     2041 attcaacaaa tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat
     2101 ggcagttatc gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg
     2161 tcggaggcct gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat
     2221 acggaaatgt ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt
     2281 cggcctttac gggcacttat agctctacgc tggctgtcag ttgctatggc gttaatttgg
     2341 gagccagtgg tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg
     2401 aacaggatga agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt
     2461 tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg
     2521 ttgaggttaa cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg
     2581 cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca
     2641 gctttaacta caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga
     2701 ttgtggggct gaacagcgcc atgttactgg tagctgaaga tggaggattt acacttgaaa
     2761 ttaacggcag cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac
     2821 tgagtgttgt aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg
     2881 tgacttattc acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat
     2941 tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc
     3001 ggcgatattg ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag
     3061 tgaaattacg tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc
     3121 gtcggcagga aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac
     3181 ctgtacagct aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg
     3241 gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc
     3301 gccgccagga tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc
     3361 ttcatgtaca gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta
     3421 ccttgagcag acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa
     3481 attacattcg aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga
     3541 ccccaaccat attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt
     3601 agacttgcat ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt
     3661 ggacatgtgc ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa
     3721 agatgaaggg cgaccagctg cagggcgcag agacggtgac ttctctatct atcgtcaagg
     3781 agggaccacc accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga
     3841 aaccggtggg caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct
     3901 gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac
     3961 acccttgact ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat
     4021 gggcaaattg tcagtaatct ttacgccttc cttgccaacc atcaattgac cccgcaggcc
     4081 agaaaaaact aaacaacgaa gccgcctgat aaggcggctt cgtcattaca acttacgtta
     4141 aacgttaaac gttaatgttg cggtgcggga tcgttatgtg tttcctgcgc cacctcaatc
     4201 gccggtttgt tctgcaatac ggtccagata accagcgcac ccagcaggtc aaatactgcc
     4261 agaactgcga acagcgggct gaagccgatg gtgtcagcca gtgcaccgac aaccagcgca
     4321 aacagcgtac ttgccagcca tgcggacatc ccggttaaac cgttcgccgt cgccacttcg
     4381 ttacgaccga acacgtcaga agagagcgta atcagcgcgc cagacagtgc ctggtgggca
     4441 aaaccaccga tacacagcag cataattgcg acatacgggt tggtgaacag accgatcata
     4501 cccgggccaa tcatcagcac agcacccagc gttacgacca tcttacggga aacgatcagg
     4561 ttcacaccaa accaacgctg gaacagcggc ggcaggtaac caccgaggat acaaccgagg
     4621 tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct ctttcaggtt aaagccgtaa
     4681 actttaaaca tgaacagcgg gatccacgcg ttaaaagtac cccaggccgg ttctgccagg
     4741 aaacgcggca gtgcgatacc ccagaactga cggttacgca ggatctgacc aacggacatt
     4801 ttcttcgccg tgctcacctg gtgctgggct tcctgaccat taataatata gtcgcgttct
     4861 tcatcggtca gatgcttctg gtcgcgcgga tgtttataga aaatcagcca tgccatcgcc
     4921 cagataaagc tcaatgcacc ggagatgata aatgccatct gccagctgtg catcacgatt
     4981 gccataccac cagcggcggc gcaatcatcg caccaatcga agaacctacg ttaaagtagc
     5041 ctactgcgat ggaacgctct ttcgccggga accattcgga gctggctttc agacccgccg
     5101 ggatcatcgc tgcttccgcg gcaccgaccg caccacgagc aacagccagg ccaccccagc
     5161 tacctgccag cgcggttgca ccacagaaca cggcccacag cacagcaaac attgcataac
     5221 cgattttcgt acccagcaca tccagtacat aacctgctac cggttgcatg accgtataag
     5281 cagcagaata ggctgcgatg atataggaat actgttgggt ggagatgttt aactcttcca
     5341 tcagagttgg cgcagctgcc gccacagtgt tacgcgtcag gtaaccaagc acggtgccga
     5401 gcgtcaccag tgcgatcata taccaacgta accctttaat tttacgcatg taaaacctca
     5461 tcgttttgtt atatccgcag gaagcgcggc catcatcccg ccagaggctg aagatgttag
     5521 ttacgaccgg gtaaccgaaa aaaaaccggc acgccccgaa cgttgccatg aaagtttcgt
     5581 gctaattcgg cttccgtacc ggttaacccg attcatctaa caaatgaatg aatcggcaat
     5641 ttgcattccg tcgacttatg atttgcgacg gcagaaagat aacttgtcat acaactttaa
     5701 aaggtgagag ccatcacaaa tgtgcgaata tttgtaggga cattacctga cgacagcgag
     5761 gccagtactg gcgcggcctg cagcgagatt tatcactttg agagtaattt tttaactacg
     5821 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     5881 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     5941 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     6001 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     6061 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     6121 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcatct gaccgtgaaa
     6181 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     6241 cgcaccttga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     6301 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     6361 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     6421 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     6481 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     6541 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     6601 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     6661 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     6721 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     6781 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     6841 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     6901 gtccgatggc ggaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     6961 cgaaaaccat tctctactgc ctgaacccgc gcgataacga agtgctgggc accatgatcg
     7021 gtaacttcta gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     7081 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtctgctga
     7141 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     7201 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     7261 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     7321 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     7381 gctggataac gtcgcggtcg ctttagcaga tttggctgaa ggcacagaag tcagtgtcga
     7441 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     7501 tatcgcaaaa ggggccaacg tcatcaaata tggcctgccg attggttatg cattggcgga
     7561 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     7621 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcgtgaagt
     7681 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     7741 aaccgtgggc tgtgtcaacg gcatcgcgcg gcaaatccag aaccgtttcc tgaaagagac
     7801 caacaacgcc gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     7861 actgggcgac gatcacatca acatccgcac catgctgcaa aacatggtgc gccacccgaa
     7921 cgcgggcgca gtgctggtga ttggtctggg ctgtgaaaac aaccaggttg ccgcattccg
     7981 tgaaacgttg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     8041 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     8101 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     8161 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     8221 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     8281 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     8341 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     8401 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     8461 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgcctg ggctgaactt
     8521 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     8581 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     8641 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggatcgact ttgacgcggg
     8701 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     8761 tgagtttgcc aacggtaagc aaacttgcaa cgagcgtaac gacttccgcg aactggcgat
     8821 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     8881 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     8941 ggctggccca gggcgtgggt gtcatcgcct ttctgattgg tatcacaaca tttttcaatc
     9001 gtgacgaacg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     9061 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     9121 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     9181 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     9241 tcagtacctg ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     9301 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     9361 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     9421 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     9481 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     9541 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     9601 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     9661 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     9721 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     9781 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     9841 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     9901 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     9961 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
    10021 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
    10081 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
    10141 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacat
    10201 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
    10261 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
    10321 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
    10381 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
    10441 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
    10501 cccatgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
    10561 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
    10621 tgatatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
    10681 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
    10741 tcattgtgtt atcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
    10801 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
    10861 tttaaagaga agtcatattt tgaaacttaa tggttacatt tgtttgacat aaacagaggg
    10921 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
    10981 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctg
//
LOCUS       DAZSBF010000136        11003 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZSBF010000136 DAZSBF010000000
VERSION     DAZSBF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.138"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..280)
                     /locus_tag="ACHXWD_002812"
     CDS             complement(<1..280)
                     /locus_tag="ACHXWD_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7621684.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(447..1187)
                     /locus_tag="ACHXWD_002813"
     CDS             complement(447..1187)
                     /locus_tag="ACHXWD_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR7621685.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHXWD_002814"
     CDS             complement(1280..1915)
                     /gene="argO"
                     /locus_tag="ACHXWD_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR7621686.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHXWD_002815"
     CDS             complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="ACHXWD_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR7621687.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            3110..3208
                     /locus_tag="ACHXWD_002816"
                     /pseudo
     CDS             3110..3208
                     /locus_tag="ACHXWD_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHXWD_002817"
     CDS             complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="ACHXWD_002817"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR7621688.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHXWD_002818"
     CDS             complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="ACHXWD_002818"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR7621689.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHXWD_002819"
     CDS             complement(5779..6798)
                     /gene="epd"
                     /locus_tag="ACHXWD_002819"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR7621690.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7170..7601
                     /locus_tag="ACHXWD_002820"
     CDS             7170..7601
                     /locus_tag="ACHXWD_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR7621691.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7624..8202
                     /locus_tag="ACHXWD_002821"
     CDS             7624..8202
                     /locus_tag="ACHXWD_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR7621692.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8203..8910
                     /locus_tag="ACHXWD_002822"
     CDS             8203..8910
                     /locus_tag="ACHXWD_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR7621693.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RQAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8898..9575
                     /locus_tag="ACHXWD_002823"
     CDS             8898..9575
                     /locus_tag="ACHXWD_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7621694.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9569..10246
                     /locus_tag="ACHXWD_002824"
     CDS             9569..10246
                     /locus_tag="ACHXWD_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7621695.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10218..10931)
                     /locus_tag="ACHXWD_002825"
     CDS             complement(10218..10931)
                     /locus_tag="ACHXWD_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR7621696.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10928..>11003)
                     /locus_tag="ACHXWD_002826"
     CDS             complement(10928..>11003)
                     /locus_tag="ACHXWD_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7621697.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2627 a         2920 c         2740 g         2716 t
ORIGIN      
        1 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
       61 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
      121 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
      181 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
      241 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
      301 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
      361 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
      421 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
      481 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
      541 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
      601 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
      661 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
      721 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
      781 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
      841 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
      901 tctggctggg tgcgtaagtt cgctgagctg atatcttttt tcgcgatctg attgagttca
      961 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
     1021 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
     1081 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
     1141 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
     1201 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
     1261 tttttccatc agatagtgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
     1321 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
     1381 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
     1441 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
     1501 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
     1561 tcactgccag catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
     1621 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
     1681 aggctacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
     1741 tgccaccaaa aatcccggcg caaatcagga ccaaatcgct gatagcacaa agtaaggcaa
     1801 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtggaccga
     1861 gcggcagaat catagctgcc ccaagagcaa taccttgaaa gtaataagaa aacacgttgg
     1921 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
     1981 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
     2041 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
     2101 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
     2161 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
     2221 acgatgcacc aagttcgttc agacgcacag tcatttcgcg atctttcaaa atgcgatctt
     2281 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
     2341 cgccaataat aaattcgtta cggcgaactg gctcgcgaga gaagttaata atatttccgg
     2401 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
     2461 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gagatcaaca tattctccgg
     2521 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
     2581 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
     2641 gtacacccac gcgtcccagg gcagcgatta gcgtaaaggc gataataccg taacggacta
     2701 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
     2761 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
     2821 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
     2881 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
     2941 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
     3001 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
     3061 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
     3121 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
     3181 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
     3241 gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg ttcagttcct
     3301 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
     3361 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac tgcccctgca
     3421 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
     3481 cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt tcctgagcag
     3541 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
     3601 tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
     3661 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
     3721 tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
     3781 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
     3841 tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca
     3901 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
     3961 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
     4021 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
     4081 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
     4141 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
     4201 cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
     4261 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
     4321 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
     4381 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     4441 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
     4501 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
     4561 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
     4621 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
     4681 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
     4741 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
     4801 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
     4861 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
     4921 gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
     4981 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
     5041 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
     5101 tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac tttagaacca
     5161 ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag cgcgtccagt
     5221 tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat accgtgagta
     5281 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
     5341 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
     5401 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccaga
     5461 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
     5521 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac tttcgcgcct
     5581 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
     5641 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
     5701 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
     5761 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
     5821 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
     5881 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
     5941 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
     6001 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
     6061 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
     6121 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
     6181 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
     6241 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
     6301 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
     6361 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
     6421 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
     6481 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
     6541 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
     6601 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
     6661 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
     6721 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
     6781 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
     6841 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
     6901 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
     6961 aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt ttcagccccg
     7021 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
     7081 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
     7141 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttccc
     7201 ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
     7261 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
     7321 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac ggtacattcg
     7381 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
     7441 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
     7501 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
     7561 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
     7621 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
     7681 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
     7741 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
     7801 ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt tgcacccgtc
     7861 gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg
     7921 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
     7981 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
     8041 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacagtgat aggcgcgaac
     8101 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
     8161 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
     8221 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
     8281 ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac gcggcggcag
     8341 gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
     8401 ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg gacgcatgct
     8461 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
     8521 gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
     8581 tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
     8641 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
     8701 gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct ggctgtccgt
     8761 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
     8821 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctaaca
     8881 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
     8941 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
     9001 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
     9061 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
     9121 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
     9181 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
     9241 ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
     9301 tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg gtcatcgcct
     9361 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt cgcctgacgc
     9421 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc
     9481 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
     9541 aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat cttatcgctg
     9601 gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
     9661 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
     9721 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
     9781 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
     9841 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
     9901 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
     9961 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    10021 agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg acgaaaattg
    10081 gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga gcgtggtagc
    10141 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    10201 tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    10261 gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc gtcggtacgg
    10321 tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg ccccaccaga
    10381 cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    10441 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    10501 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta ctgcggccac
    10561 gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac gtcaaatgtc
    10621 tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt gtaatggtga
    10681 aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg cacgagatat
    10741 tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    10801 gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    10861 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    10921 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    10981 tgaccattcc gctaaaacgc gcc
//
LOCUS       DAZSBF010000137        10863 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZSBF010000137 DAZSBF010000000
VERSION     DAZSBF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.139"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            32..529
                     /gene="ftnA"
                     /locus_tag="ACHXWD_002827"
     CDS             32..529
                     /gene="ftnA"
                     /locus_tag="ACHXWD_002827"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR7621698.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(567..806)
                     /locus_tag="ACHXWD_002828"
     CDS             complement(567..806)
                     /locus_tag="ACHXWD_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR7621699.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            997..2208
                     /gene="tyrP"
                     /locus_tag="ACHXWD_002829"
     CDS             997..2208
                     /gene="tyrP"
                     /locus_tag="ACHXWD_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR7621700.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2270..2935)
                     /gene="yecA"
                     /locus_tag="ACHXWD_002830"
     CDS             complement(2270..2935)
                     /gene="yecA"
                     /locus_tag="ACHXWD_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR7621701.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3131..3217)
                     /locus_tag="ACHXWD_002831"
     tRNA            complement(3131..3217)
                     /locus_tag="ACHXWD_002831"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3181..3183),aa:Leu,seq:taa)
     gene            complement(3230..3303)
                     /locus_tag="ACHXWD_002832"
     tRNA            complement(3230..3303)
                     /locus_tag="ACHXWD_002832"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3269..3271),aa:Cys,seq:gca)
     gene            complement(3358..3433)
                     /locus_tag="ACHXWD_002833"
     tRNA            complement(3358..3433)
                     /locus_tag="ACHXWD_002833"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3398..3400),aa:Gly,seq:gcc)
     gene            complement(3585..4133)
                     /gene="pgsA"
                     /locus_tag="ACHXWD_002834"
     CDS             complement(3585..4133)
                     /gene="pgsA"
                     /locus_tag="ACHXWD_002834"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR7621702.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4190..6022)
                     /gene="uvrC"
                     /locus_tag="ACHXWD_002835"
     CDS             complement(4190..6022)
                     /gene="uvrC"
                     /locus_tag="ACHXWD_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR7621703.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6019..6675)
                     /gene="uvrY"
                     /locus_tag="ACHXWD_002836"
     CDS             complement(6019..6675)
                     /gene="uvrY"
                     /locus_tag="ACHXWD_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR7621704.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6971..7147
                     /gene="yecU"
                     /locus_tag="ACHXWD_002837"
     CDS             6971..7147
                     /gene="yecU"
                     /locus_tag="ACHXWD_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR7621705.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7134..7358
                     /gene="yecF"
                     /locus_tag="ACHXWD_002838"
     CDS             7134..7358
                     /gene="yecF"
                     /locus_tag="ACHXWD_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR7621706.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7425..8147)
                     /gene="sdiA"
                     /locus_tag="ACHXWD_002839"
     CDS             complement(7425..8147)
                     /gene="sdiA"
                     /locus_tag="ACHXWD_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR7621707.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8377..9129)
                     /gene="tcyN"
                     /locus_tag="ACHXWD_002840"
     CDS             complement(8377..9129)
                     /gene="tcyN"
                     /locus_tag="ACHXWD_002840"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR7621708.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9126..9794)
                     /gene="tcyL"
                     /locus_tag="ACHXWD_002841"
     CDS             complement(9126..9794)
                     /gene="tcyL"
                     /locus_tag="ACHXWD_002841"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR7621709.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9809..10795)
                     /gene="dcyD"
                     /locus_tag="ACHXWD_002842"
     CDS             complement(9809..10795)
                     /gene="dcyD"
                     /locus_tag="ACHXWD_002842"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR7621710.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2684 a         2829 c         2571 g         2779 t
ORIGIN      
        1 gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga ttgaaaaact
       61 taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa tgagcgcctg
      121 gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg cccaggaaga
      181 gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt taccgcgtat
      241 taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat tccaggaaac
      301 ctataaacac gaacaattaa tcacccagaa aattaacgaa ctggctcatg ctgcaatgac
      361 caatcaggac tacccaacat ttaatttcct gcagtggtat gtttctgagc agcatgaaga
      421 agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa gcggcgaagg
      481 tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat gctctccggc
      541 ggcaggttta ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa
      601 aaaccgtctt tcgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc
      661 gctgtcatcc cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa acgcgcttta
      721 atcgcagctt ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac
      781 acttcatgac cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt
      841 gccgcttatg aagactttgc gccagcgcag gactgaatgc tttttattgt acatttatat
      901 ttacactata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc tgcgccgcag
      961 atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac cctgggaagt
     1021 gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc gctggctgcg
     1081 gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc gttgatgtgc
     1141 tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac cggtctgggc
     1201 acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt cagtatgatg
     1261 ttcttaatgt atgctctgac tgcggcatac atcagcggtg tcggtgaatt gttggcctcc
     1321 agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct gttgttcact
     1381 tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt taaccgtttt
     1441 ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct accgcatatt
     1501 cacaaagtga atcttttaac cctgccgttg caacaggggc tggctctgtc agcaatcccg
     1561 gtgattttta cctcatttgg ttttcacggt agcgtgccga gtattgtcag ctatatggat
     1621 ggcaacgttc gtaagctacg ctgggtgttt ataaccggta gtgcgatccc cctggtggca
     1681 tatattttct ggcaggtggc aacgcttggc agcattgatt caacaacctt tatgggattg
     1741 ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat ggtggcctct
     1801 ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac gtcatttctc
     1861 ggcgttgcgt taggcttatt tgattatctg gctgatttat ttcagcgttc aaataccgtt
     1921 ggtggacggt tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt tgcactgttt
     1981 tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc ggtactggca
     2041 ttgattatcc cttcgctgtt gacctggcaa agcagaaagc acaatcctca ggcgggttac
     2101 cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat tgctgtgatt
     2161 ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc agatagcctc
     2221 aaattcctta ttgggtgcca gaattaacgc tgacacccaa tttggcctct taatgcaggc
     2281 agcactgctt aaatttctta ccactaccgc acgggcaagg atcgttacgc cccggtttct
     2341 cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc cagtacgcat
     2401 gtagatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct tctggcgaca
     2461 tcttttctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc tccagcgctg
     2521 gttttaacga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc atatagccaa
     2581 agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag ccgaataacg
     2641 gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata tgttgaaaag
     2701 ccaggttcat aaagcgcgtc atctctttct ctgacgccca gcgcggcaca tagtcagccc
     2761 caccccacac ggcaaccagc cactgttccg gttcaatctc ttgcggagaa ctcaacaccg
     2821 ccgtcaataa accgtccagc tccgccacat caaggatggc gtggttagtg ttgtatttgg
     2881 tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt ttcatacgct
     2941 tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt atcagagata
     3001 ctttttgagt ggctttgccg gtgattaaaa attaaggagg gtgtaacgac aagttgcagg
     3061 cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attgctttga ttttattctt
     3121 atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg ccgagggatt
     3181 ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt ggaggcgcgt
     3241 tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct ttgttaacgc
     3301 gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct tttaaactgg
     3361 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
     3421 gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta tcgtctggca
     3481 atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag tcaacgatat
     3541 ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg atcaagcaaa
     3601 tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac cgcagccaca
     3661 aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag cagccatgcc
     3721 aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc cacgctactg
     3781 cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc acgggcgatc
     3841 atcgttgctg ccggtaaggt cacccaccag ctgtggtaat gctcggttac cagcaccatg
     3901 gcgatagcca cgagaacttt atctgctaca gggtcaagga aagcaccaaa ccgggtactc
     3961 tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc gacgcagaaa
     4021 atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa tacaaagaat
     4081 gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg cataatgacg
     4141 ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat caatgtttca
     4201 acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt gcaatttcct
     4261 cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac atttgccgac
     4321 gttttggccc gacgccttca atggtttcca gggaactggt atttttgacc ttcgcccgtt
     4381 ttttacggtg cccgccaatc gcgtgatcat gtgattcatc gcgaatatgc tggataacat
     4441 gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca aagaacagcg
     4501 tttccagccc agccttacga tctgctcctt tggcaacgcc aagtagcagc ggatgatttt
     4561 tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc tggcctttgc
     4621 cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta ccataacgcc
     4681 gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct gtaatgttat
     4741 agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag gaagcgacgg
     4801 tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact tccggcaatt
     4861 tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc gaaagtttgc
     4921 tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc ctatcgccgc
     4981 gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa tcggcgagca
     5041 gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg cgcatctggc
     5101 tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt tccgtaccgc
     5161 caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga atgaacaata
     5221 cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg tcgtcgccgg
     5281 tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg tcgcgaatac
     5341 gtgcagcttc ttcaaactcc agattctggc tggcagtttc catacgacta atgagttgcg
     5401 taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcaacc tgctgagcgt
     5461 attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct atctgatatt
     5521 gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga atggggaaaa
     5581 tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac gggccgaaat
     5641 attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg gtatcgccac
     5701 tcaggaagat aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa cgcggctgat
     5761 agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtatgc gtaaccgtta
     5821 catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg aggttgctgc
     5881 ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag ataaccgtac
     5941 caccagcatc gtacatgcga taaacgcctg gctggcaggt tacggttttt aaaaacgctt
     6001 ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca gaccatggcg
     6061 aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga acatacgata
     6121 gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct cattgacctt
     6181 ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac tggcaaatgg
     6241 gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga tatcagaagc
     6301 aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct gcggagccgc
     6361 gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta aagggttttc
     6421 tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga ttttacgcgt
     6481 cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt caacggcatt
     6541 tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga cttttatacc
     6601 ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt catcaacaag
     6661 tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga tagttagtca
     6721 ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg gtattcgatt
     6781 tttctctcgt ttttgtaaat tcgattgtcc acagttatgg tcacgcccgt caccaacggc
     6841 ttccgggatc gtttcacagt aagcccggcg atgaaatgtg acaaaaattg actttattca
     6901 gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac aatacgggca
     6961 aaaaacattc atgataaaaa tatttattgg tcattatatt aacgcattta atagcattgt
     7021 tgacgtcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg tatactccgc
     7081 cgccttaaca tttttcaccg caaattttca ttgcaacatg acgaggaaaa taaatgagta
     7141 cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc tcctgcctga
     7201 aagcgatgct gacgctgatg ctacaggcga tgggacaagc tgacgcgggc cgcgtgatgt
     7261 taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca gtattttcca
     7321 aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc tgagagcgtg
     7381 cctttcagcc gatttttgca tctggcacgc agaaagaaaa gcgatcaaat taagccagta
     7441 gcggccgcgt aacaggccac ctgggtctta tttggtgcat tgattttttt ctgcatgttt
     7501 ttctgatgga aattgaccgt attctcagag attgacaaaa tcatcgctat ctctgctgat
     7561 gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa attcatctct
     7621 ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc gcgcaccagt
     7681 aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt gctgcaacgg
     7741 gaaaaggaca aaaagcccag cgcccgattt ggcagcatta aatactgagt gacaccgcgg
     7801 cgtaaaccat gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa taagtcatca
     7861 ttccacatta aatggccctg actaaagttt tcagggttca gcacaggatc aattgcgaga
     7921 aaattttttg cctgataata actaacccac gactcagggt aattggtgta gaaagccact
     7981 ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc gtactccagc
     8041 tgctgagcct gaagctctat ttcatggtag acctcttctg cggcctccat cctctgaaaa
     8101 cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt aaaccgcaac
     8161 gcccctgaga gtgagtatca tttataatga taattcaaac tatcacataa ataagattta
     8221 tatataattt atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg
     8281 ttattaatga gttatgctga tttgttaagc agttttatca cgcttgaaat ggcgtcccgc
     8341 cccgacaggt gaatcgtcgg ggctgatttt ttcttattat tgcagtagaa acttctcgag
     8401 gaactggcgg gtgcgaggct gctggggatc agcaaataac gcttttgctg ccccctgctc
     8461 gactatccgc ccctggtcca taaaaatcgc ccggtccgca acatcccggg caaagctcat
     8521 ttcgtgcgtc acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga ttgtgttcag
     8581 gacttcgccc accagctctg gatccagcgc tgacgttggc tcgtcaaaca aaatcacctc
     8641 aggacgcatt gccagcgcac gcgcaatcgc tacacgctgc tgttgaccgc cagacaaacg
     8701 acgtggatag ctggtttctt taccggccag cccaactttc gccagcagct cgcgagcgcg
     8761 cgccgtggcc tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc
     8821 cagcaccgta cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg
     8881 caactggcgg atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc
     8941 gccaacggtg atcgttcccg cttccggttg ttccagcaga tttatgctgc gtagcaacgt
     9001 ggttttgccg gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag
     9061 gtcgataccg tgcagcaccg tctgaccgtg aaattttttc accaggttct taacttcaat
     9121 ggcactcatt ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac
     9181 gtcgataata ctgtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac
     9241 acctccagcg tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt
     9301 gtcgcggcca gcgacgtatc ctttaccagg ctgataaagc tgttcgacag cggcggcaac
     9361 gccacccgcg ccgcctgtgg caaaatcgcg cgacgcatag tctgccatgg cgtcatacca
     9421 atactggcag ctgcttccca ctgaccttta tcaatagaag aaatggcggc gcgcagtgtt
     9481 tcggcggcat aggcggcagt gttcagcgac agaccaatca ttgctgacgg aatgggatct
     9541 aattcgatac caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggt
     9601 gtaccacgaa aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac
     9661 aggcgcatca gcgcgagaat aaacccgagc agtaagccaa aaaacatgcc gccgatactg
     9721 agttgcagcg tatacccagc ccctttcagc aggaacggca aggaatcaat aaccagttgt
     9781 atactttctt gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag
     9841 gcgcaccgcc ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga
     9901 tacagccaat cagccccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt
     9961 ccagccgcgc cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag
    10021 gtgcaaaata gtcatcccag agtataattt ccgctgatgc ggtcagctcc agttctttcg
    10081 caatcgcctg ttgtaggtta accactttcg gcaactgatc ggcaacggaa cgcgacacgg
    10141 tcacgccaat cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt
    10201 gagttccggc actgcccgat gcgactacca ccgacgaaat attaaccgcc ccttcacact
    10261 gttgcgcgat ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc
    10321 caaccggaat gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agctcttcca
    10381 gttgggcatt gggatcggtc agtgcttcgc acatttcaat ctgggtattg aacagatcca
    10441 gcaacaagcg attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca
    10501 gcagcgccac acagtgcaga ccgagtttcg ccgcaactgc ggcagtctgc cgcacatggt
    10561 tagactggat caccccggca gtaatcagcg tatcggcacc ttcgcgcaga gcatctgccg
    10621 cgagaaattc tagcttacgt aatttattgc cgcccattgc catgggggtg acgtcatccc
    10681 gtttgatgaa aatttcccgt cctagataat cagaaaagcg cggcagatat tcgagcggcg
    10741 ttggcgcgcc gataaactcc aaccgtggaa aacgggttaa attatgcagt ggcataacag
    10801 cctccgatgt atgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa
    10861 agg
//
LOCUS       DAZSBF010000138        10757 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZSBF010000138 DAZSBF010000000
VERSION     DAZSBF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10757
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.140"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHXWD_002843"
     CDS             92..1132
                     /locus_tag="ACHXWD_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR7621711.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHXWD_002844"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHXWD_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR7621712.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHXWD_002845"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHXWD_002845"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR7621713.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHXWD_002846"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHXWD_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR7621714.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHXWD_002847"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHXWD_002847"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR7621715.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHXWD_002848"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHXWD_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR7621716.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="ACHXWD_002849"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="ACHXWD_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHXWD_002850"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="ACHXWD_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR7621717.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         2742 a         2991 c         2618 g         2406 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggat
//
LOCUS       DAZSBF010000139        10716 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZSBF010000139 DAZSBF010000000
VERSION     DAZSBF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.141"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="ACHXWD_002851"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="ACHXWD_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="ACHXWD_002852"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="ACHXWD_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR7621718.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="ACHXWD_002853"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="ACHXWD_002853"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR7621719.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="ACHXWD_002854"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="ACHXWD_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /gene="ybjH"
                     /locus_tag="ACHXWD_002855"
     CDS             complement(4104..4388)
                     /gene="ybjH"
                     /locus_tag="ACHXWD_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR7621720.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="ACHXWD_002856"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="ACHXWD_002856"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR7621721.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="ACHXWD_002857"
     CDS             complement(5289..6497)
                     /locus_tag="ACHXWD_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7621722.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="ACHXWD_002858"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="ACHXWD_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR7621723.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="ACHXWD_002859"
     CDS             complement(7292..8977)
                     /locus_tag="ACHXWD_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR7621724.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="ACHXWD_002860"
     CDS             9247..9624
                     /locus_tag="ACHXWD_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR7621725.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="ACHXWD_002861"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="ACHXWD_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR7621726.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="ACHXWD_002862"
     CDS             10071..10358
                     /locus_tag="ACHXWD_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR7621727.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..>10716
                     /gene="nfsA"
                     /locus_tag="ACHXWD_002863"
     CDS             10342..>10716
                     /gene="nfsA"
                     /locus_tag="ACHXWD_002863"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR7621728.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLG"
BASE COUNT         2765 a         2650 c         2713 g         2588 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggc
//
LOCUS       DAZSBF010000140        10713 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZSBF010000140 DAZSBF010000000
VERSION     DAZSBF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.142"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..454
                     /locus_tag="ACHXWD_002864"
     CDS             <1..454
                     /locus_tag="ACHXWD_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7621729.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            670..759
                     /locus_tag="ACHXWD_002865"
                     /pseudo
     CDS             670..759
                     /locus_tag="ACHXWD_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHXWD_002866"
     CDS             complement(760..1818)
                     /gene="modC"
                     /locus_tag="ACHXWD_002866"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR7621730.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHXWD_002867"
     CDS             complement(1821..2510)
                     /gene="modB"
                     /locus_tag="ACHXWD_002867"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR7621731.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHXWD_002868"
     CDS             complement(2510..3283)
                     /gene="modA"
                     /locus_tag="ACHXWD_002868"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7621732.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHXWD_002869"
     CDS             complement(3449..3598)
                     /gene="acrZ"
                     /locus_tag="ACHXWD_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR7621733.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3727..4515
                     /gene="modE"
                     /locus_tag="ACHXWD_002870"
     CDS             3727..4515
                     /gene="modE"
                     /locus_tag="ACHXWD_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR7621734.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4583..6055
                     /gene="modF"
                     /locus_tag="ACHXWD_002871"
     CDS             4583..6055
                     /gene="modF"
                     /locus_tag="ACHXWD_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR7621735.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6316..7332
                     /gene="galE"
                     /locus_tag="ACHXWD_002872"
     CDS             6316..7332
                     /gene="galE"
                     /locus_tag="ACHXWD_002872"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR7621736.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7342..8388
                     /gene="galT"
                     /locus_tag="ACHXWD_002873"
     CDS             7342..8388
                     /gene="galT"
                     /locus_tag="ACHXWD_002873"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR7621737.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8392..9540
                     /gene="galK"
                     /locus_tag="ACHXWD_002874"
     CDS             8392..9540
                     /gene="galK"
                     /locus_tag="ACHXWD_002874"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR7621738.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9534..10574
                     /gene="galM"
                     /locus_tag="ACHXWD_002875"
     CDS             9534..10574
                     /gene="galM"
                     /locus_tag="ACHXWD_002875"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR7621739.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
BASE COUNT         2696 a         2850 c         2715 g         2452 t
ORIGIN      
        1 cggcctttgc atggatcttg cactggtaac aggacagcgc gaaggagatc tggcggctat
       61 gagatgggag gatataagag atggcaggct atacgtggaa caacaaaaaa caggggcaaa
      121 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      181 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      241 gacaatcgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      301 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      361 cggtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      421 tgacggacga gggagggaat ggaaagacat ttaaaattaa tttcgaatga tttcgaaaca
      481 ccacttgtaa ttaattgatt ataaatgatt aattcctaat gggcatttgc ttcatgaatg
      541 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      601 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      661 aaattcttaa atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
      721 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
      781 tttaatttgc gcgtacagcc acaggccagg tttgatcgcc agttcatccc tggcccacgg
      841 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctggccgtt
      901 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
      961 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1021 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1081 gtaatgcgga tgatgctcaa gtaccgtcac tttcagaatg ctactttgtt gctctttcgg
     1141 cagccacgga ttcatcacgc tactgcccca cacttcctcc agcgcgccaa aggctttcac
     1201 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     1261 gctgacatac aacatcggaa tgttgatttc ccgtgtcagc cgttgcagat aaggcaacag
     1321 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattccgg
     1381 tgctgtcagc aaagcccgac caatcgccac gcgctgtttt tcgcctccgg acaggctgcc
     1441 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     1501 gaccatactt ttcgacatgc cgtagcgcag attgccacgc actttgtaat gcgggaacag
     1561 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttcc ggcgtcaggc agataccttt
     1621 ttcggcatca tttagtaccc gcccattgag gacaatccgc cctttttgcg ggcgcgtcag
     1681 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     1741 gatgccattg gcgggcagtg tttcattaat ggtcaggcaa tggttgccca acgtctggga
     1801 aaaattcagc tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     1861 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     1921 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     1981 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2041 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     2101 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     2161 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     2221 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     2281 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     2341 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     2401 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     2461 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     2521 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     2581 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     2641 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     2701 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     2761 cttccgccgg agccagtttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     2821 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     2881 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     2941 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3001 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3061 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     3121 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     3181 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     3241 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     3301 aacgttatat aggtgacaat gtaacggcta gctcagaatt ttcccagcag ttattgctaa
     3361 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     3421 atgcccgact aagcgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     3481 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     3541 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     3601 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     3661 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     3721 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     3781 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     3841 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     3901 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     3961 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4021 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4081 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     4141 gatcatgatg acattcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     4201 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     4261 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     4321 gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     4381 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     4441 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     4501 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     4561 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     4621 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     4681 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     4741 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     4801 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     4861 tggcgaagat gacaccggac gcactacggc tgaaatcatt caggatgaag taaaggatgc
     4921 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     4981 taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc tgatgtcgga
     5041 gcctgacttg ttgattcttg atgagtcgtt cgatggcctg gatgttgcct cacgtcagca
     5101 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     5161 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     5221 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcgca
     5281 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     5341 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     5401 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     5461 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     5521 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     5581 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     5641 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     5701 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     5761 tacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg cgctgatcgt
     5821 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     5881 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     5941 gcaattgtta tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6001 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6061 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     6121 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     6181 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     6241 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     6301 ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat tggaagtcat
     6361 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     6421 agtaagcgca gcgtactgcc tgttatcgag cgcttaggcg gcaaacatcc aacgtttgtt
     6481 gaaggcgata ttcgtaacga agcgttgatg accgagatac tgcacgatca cgctatcgac
     6541 accgtgatcc acttcgccgg gctgaaagcc gtcggcgaat cggtacaaaa accgctggaa
     6601 tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg cgccgctaac
     6661 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     6721 tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag caaattgatg
     6781 gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat tgccctgctg
     6841 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     6901 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     6961 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7021 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7081 gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt ggttaatgcc
     7141 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgaaggcgac
     7201 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     7261 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     7321 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     7381 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     7441 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     7501 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac cgggacttac
     7561 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     7621 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     7681 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     7741 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     7801 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     7861 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     7921 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     7981 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8041 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     8101 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     8161 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     8221 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     8281 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     8341 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     8401 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     8461 caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga cggtttcgtt
     8521 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     8581 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     8641 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     8701 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     8761 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     8821 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     8881 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     8941 gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc catgccgaaa
     9001 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9061 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agctctgcgc
     9121 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
     9181 cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
     9241 caaggcgatc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
     9301 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agcagtgatt
     9361 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgctg
     9421 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
     9481 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
     9541 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
     9601 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gctcgtattc
     9661 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
     9721 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
     9781 gccgttatac ctttgacggt gaaacggtga cgctttcgcc aagtcagggc gttaaccagc
     9841 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
     9901 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
     9961 gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10021 cagttgataa atcttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10081 agcagtctga cgtgcgcaat cacaagttgc agattctggc gggcgaatat ctgccggttg
    10141 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacctct tttgatttcc
    10201 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    10261 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    10321 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccc gctcttcaat
    10381 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct tacgccgact
    10441 ggcaaggact ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    10501 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    10561 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat tgttcaaatc
    10621 accagcaaac accgacatat ttgcaactca atattcacaa caaccttaca ctgcgccact
    10681 attttcgcta tggttatgcg taagcattgc tgt
//
LOCUS       DAZSBF010000141        10686 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZSBF010000141 DAZSBF010000000
VERSION     DAZSBF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.143"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHXWD_002876"
     CDS             complement(<1..784)
                     /locus_tag="ACHXWD_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR7621740.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHXWD_002877"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHXWD_002877"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR7621741.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHXWD_002878"
     CDS             1860..2747
                     /locus_tag="ACHXWD_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR7621742.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHXWD_002879"
     CDS             2780..3790
                     /locus_tag="ACHXWD_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR7621743.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHXWD_002880"
     CDS             3860..5293
                     /locus_tag="ACHXWD_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7621744.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHXWD_002881"
     CDS             5392..6741
                     /locus_tag="ACHXWD_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7621745.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHXWD_002882"
     CDS             6731..7618
                     /locus_tag="ACHXWD_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR7621746.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHXWD_002883"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHXWD_002883"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR7621747.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHXWD_002884"
     CDS             8352..9224
                     /locus_tag="ACHXWD_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR7621748.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHXWD_002885"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHXWD_002885"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR7621749.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHXWD_002886"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHXWD_002886"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR7621750.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2461 a         2532 c         2855 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccggg taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZSBF010000142        10430 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZSBF010000142 DAZSBF010000000
VERSION     DAZSBF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.144"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHXWD_002887"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHXWD_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR7621751.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHXWD_002888"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHXWD_002888"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR7621752.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHXWD_002889"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHXWD_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR7621753.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHXWD_002890"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHXWD_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR7621754.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHXWD_002891"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHXWD_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHXWD_002892"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHXWD_002892"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR7621755.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVTAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHXWD_002893"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHXWD_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR7621756.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHXWD_002894"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHXWD_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR7621757.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHXWD_002895"
     CDS             complement(9457..10149)
                     /locus_tag="ACHXWD_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR7621758.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2550 a         2516 c         2754 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tgactgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt
//
LOCUS       DAZSBF010000143        10310 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZSBF010000143 DAZSBF010000000
VERSION     DAZSBF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.145"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            446..1423
                     /gene="glaH"
                     /locus_tag="ACHXWD_002896"
     CDS             446..1423
                     /gene="glaH"
                     /locus_tag="ACHXWD_002896"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR7621759.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1443..2711
                     /gene="lhgO"
                     /locus_tag="ACHXWD_002897"
     CDS             1443..2711
                     /gene="lhgO"
                     /locus_tag="ACHXWD_002897"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR7621760.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2734..4182
                     /gene="gabD"
                     /locus_tag="ACHXWD_002898"
     CDS             2734..4182
                     /gene="gabD"
                     /locus_tag="ACHXWD_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR7621761.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4196..5476
                     /gene="gabT"
                     /locus_tag="ACHXWD_002899"
     CDS             4196..5476
                     /gene="gabT"
                     /locus_tag="ACHXWD_002899"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR7621762.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5787..7187
                     /gene="gabP"
                     /locus_tag="ACHXWD_002900"
     CDS             5787..7187
                     /gene="gabP"
                     /locus_tag="ACHXWD_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR7621763.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7208..7870
                     /gene="csiR"
                     /locus_tag="ACHXWD_002901"
     CDS             7208..7870
                     /gene="csiR"
                     /locus_tag="ACHXWD_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR7621764.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7871..8320)
                     /gene="kbp"
                     /locus_tag="ACHXWD_002902"
     CDS             complement(7871..8320)
                     /gene="kbp"
                     /locus_tag="ACHXWD_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR7621765.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8404..8562)
                     /locus_tag="ACHXWD_002903"
     CDS             complement(8404..8562)
                     /locus_tag="ACHXWD_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR7621766.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8745..9044
                     /locus_tag="ACHXWD_002904"
     CDS             8745..9044
                     /locus_tag="ACHXWD_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR7621767.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9054..9578
                     /gene="ygaP"
                     /locus_tag="ACHXWD_002905"
     CDS             9054..9578
                     /gene="ygaP"
                     /locus_tag="ACHXWD_002905"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR7621768.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9625..10029)
                     /gene="stpA"
                     /locus_tag="ACHXWD_002906"
     CDS             complement(9625..10029)
                     /gene="stpA"
                     /locus_tag="ACHXWD_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR7621769.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2409 a         2623 c         2773 g         2505 t
ORIGIN      
        1 ctggtaaagt atttacgctt agaggcggaa ctctaaatat caatattcct gggcgtagcg
       61 ctcttctttt agggaaaaca ggagaaccgc cgaactatct ctatttataa tttaatgtta
      121 tatctgcccc gataaaacgg ggcagataat atgtttagtt tactaacggt cattttgcag
      181 tgaagccatt tactgttttt tatcgaccag ataatctgtt ctctaatgtt aactccccct
      241 aacctgttgc tttagttatt catttcctgt ctcactttgc cttaatactc tacgttaaat
      301 gttactaatt tgttgctttt gatcacaata agaaaacaat atgtcgcttt tgtgcgcatt
      361 tttcagaaat gtagatattt ttagattatg gctacgaaat gagcatcgcc atgtcaccct
      421 acatctcata agaggatcgc ttctgatgaa tgcactgacc gccgtacaaa ataacgctgt
      481 cgattcaggc caggactata gcggattcac tctcatcccg tcggcgcaat ccccgcgtct
      541 gctggaactc accttcaccg aacagacgac caaacagttt ctcgagcagg ttgccgaatg
      601 gcccgtacag gcgctggagt acaaatcgtt tctgcgtttt cgggtaggca aaattcttga
      661 cgatctgtgt gcgaatcagc tgcaaccatt gctgttgaag accctgctaa accgtgctga
      721 aggtgcgctg ttgatcaatg cggtgggtat cgatgatgtg gcgcaggcag atgagatggt
      781 gaagctggca acggcggtag cgcatctgat tggtcgctca aatttcgatg cgatgagcgg
      841 tcagtattac gcgcgtttcg tggtgaaaaa tgtcgataac tcagacagct atctgcgtca
      901 gccgcaccgc gtgatggaac tgcacaacga cggcacgtat gtcgaagaga tcaccgatta
      961 cgtgctgatg atgaaaatcg atgagcaaaa catgcagggt ggaaattcgt tgctgctgca
     1021 tctcgatgac tgggaacatc tggaccacta tttccgccat ccactggcgc gtcgcccgat
     1081 gcgctttgcc gcgccgccga gcaaaaacgt cagcaaagat gttttccatc cagtgttcga
     1141 cgttgatcaa cagggtcgcc cggtgatgcg ctatatcgac cagttcgtcc agccaaaaga
     1201 ttttgaagaa ggcgtgtggt tgagcgagct ttccgatgcc attgaaacca gcaaaggcat
     1261 tctttctgta cccgttcccg ttggcaaatt cctgttgata aacaacctgt tctggctgca
     1321 cggtcgcgac cgctttactc cgcacccgga tctgcgccgt gaactgatgc gtcagcgtgg
     1381 ctatttcgct tacgccactc accactacca gacgcatcag taagcgcaaa ggaattgagc
     1441 ggatgtatga ttttgtgatt attggcggcg gcatcatcgg catgtcgacc gccatgcaac
     1501 tgattgatgt ttatccggat gcccgcattg cgttgctgga aaaagagtcc ggcccggcct
     1561 gtcaccagac gggccacaac agcggcgtga tccatgccgg ggtctattac acgcccggca
     1621 gcctgaaggc gcagttttgc ctggcgggga accgcgccac caaagccttt tgcgatcaaa
     1681 acggcattcg ctatgacaac tgcggcaaga tgctggtcgc cacgtctcag ctcgaaatgg
     1741 aacggatgcg cgccttatgg gaacgcacag cggcgaacgg tatcgagcgc gagtggataa
     1801 acgccgtgga actgcgcgag cgcgaaccga atatcaccgg gcttggtggc atttttgtgc
     1861 cgtccagcgg cattgtcagc taccgcgaag tcacagcggc aatggcaaaa atcttccagg
     1921 ccagaggcgg cgagattatt tataacgccg aagtcagcgc cctcagtaag cataaaaacg
     1981 gcgtggtgat acgcacccgt cagggcggtg aatatgaagc atcgacgctg attagctgtt
     2041 ccgggctgat ggctgaccgg ctggtgaaaa tgctcggcct cgaaccgggc tttatcatct
     2101 gcccgttccg tggtgagtat ttccgccttg cgccggagca taaccagatt gttaaccacc
     2161 tgatttaccc cattcccgac cccgcgatgc catttttggg cgtgcatctc acccgcatga
     2221 tcgacggcag cgtgaccgtc gggccaaacg cggtgctggc tttcaaacgc gaaggctatc
     2281 gcaagcgcga cttctcattt agtgacacgc tggagatttt gagctcgtcg gggattcgcc
     2341 gggtgctgca aaaccatcta cgctcaggac tgggcgagat gaaaaactcg ctgtgcaaaa
     2401 gcggctacct gcggctggtg caaaagtatt gtccccggct ttcgttaagc gatctccagc
     2461 cctggcccgc cggtgtgcgg gcgcaggcag tatcgccgga cggcaagctg attgacgatt
     2521 ttctgtttgt caccacccca cgcacgatcc acacctgcaa tgcgccctcc ccggcagcga
     2581 catcagcaat tcctattggt gcgcatattg tcagcaaggt acaaacgctg ttggcaagcc
     2641 agagtaaccc cggacgcacg ctgcgagcgg cacgtagtgt ggatgcctta cacgccgcat
     2701 ttaatcaata acctttgaaa acaggacgta acaatgaaac ttaacgacag taacttattc
     2761 cgccagcagg cgttgattaa cggggaatgg ctggacgcca acaatggcgg gatcatcgac
     2821 gtcaccaatc cggcgaacgg cgacaagctg ggtagcgtgc cgaaaatggg cgctgatgaa
     2881 acccgcgccg ctatcgacgc cgccaaccgc gccctgcccg cctggcgtgc gctcaccgcc
     2941 aaagaacgcg ccaacattct gcgcaactgg ttcaatttga tgatggagca tcaggacgat
     3001 ttagcgcgcc tgatgaccct cgaacagggc aaaccactgg ccgaagcgaa aggcgaaatc
     3061 agctacgctg cctcttttat tgagtggttt gccgaagaag gcaaacgtat ttatggcgat
     3121 accattcccg gtcatcaggc cgataagcgc ctgattgtta tcaagcagcc gattggcgtt
     3181 accgcagcta tcacgccgtg gaacttcccg gcggcgatga ttacccgtaa agccggtccg
     3241 gcgctggcgg caggctgcac catggtgctg aagcccgcca gtcagacacc gttctctgcg
     3301 ctggcgctgg cggagctggc gattcgcgcg ggcattccgg ctggcgtgtt taacgtagtc
     3361 accggttcgg caggcgcggt cggtaatgaa ctgaccagca acccgctggt acgcaaactg
     3421 tcgtttaccg gttcgaccga aattggccgc cagttaatgg aacagtgcgc gaaagacatc
     3481 aaaaaagtgt cgctggagct gggcggcaac gcgccgttta tcgtctttga cgatgccgat
     3541 ctcgataaag ccgtggaagg cgcgctggcc tcgaaattcc gcaacgccgg gcaaacctgc
     3601 gtctgcgcca accgtttata cgtgcaggac ggcgtgtatg accgctttgc cgaaaaattg
     3661 cagcaggcgg tgagcaaact gcacatcggc gacgggctgg ataaaggcgt caccatcggg
     3721 ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc atattgccga tgcgctggag
     3781 aaaggtgcgc gtgtggtttg cggcggtaaa gcggatgaac gcggcggcaa cttcttccag
     3841 ccgaccattc tggtggacgt gccggccaac gccaaagtgt cgaaagaaga gacgttcggc
     3901 cccctcgccc cgctgttccg ctttaaagat gaagccgatg tgattgcgca agccaatgac
     3961 accgagtttg gccttgccgc ctatttctac gcccgtgatt taagccgcgt cttccgcgtg
     4021 ggcgaagcgc tggagtacgg catcgtcggc atcaataccg gcattatttc caatgaagtg
     4081 gccccgttcg gcggcatcaa agcctcgggt ctgggtcgtg aaggttcgaa gtatggcatc
     4141 gaagattact tagaaatcaa atatatgtgc atcggtcttt aactggagaa tgcgaatgaa
     4201 cagcaataaa gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg ttgggcaaat
     4261 tcacccgatt ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg aaggccgtga
     4321 gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc atccgaaggt
     4381 ggtggccgca gtggaagcgc agttgaaaaa actgtcgcac acctgcttcc aggtgctggc
     4441 ttacgagccg tatctggagc tgtgcgagat tatgaatcag aaggtgccgg gcgatttcgc
     4501 caagaaaacg ctgctggtta cgaccggttc cgaagcggtg gaaaacgcgg taaaaatcgc
     4561 ccgcgccgcc accaaacgta gcggcaccat cgcttttagc ggcgcgtatc acgggcgcac
     4621 gcattacacg ctggcgctga ccggcaaggt gaatccgtac tctgcgggca tggggctgat
     4681 gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg cacggcataa gcgaggatga
     4741 cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc gcgccggaag atatcgccgc
     4801 catcgtgatt gagccggttc agggcgaagg cggtttctac gccgcgtcgc cagcctttat
     4861 gcagcgttta cgcgctctgt gtgacgagca cgggatcatg ctgattgccg atgaagtgca
     4921 gagcggcgcg gggcgtaccg gcacgctgtt tgcgatggag cagatgggcg tggcgccgga
     4981 tcttaccacc tttgcgaaat cgatcgcagg cggtttcccg ctggcgggcg tcaccgggcg
     5041 cgcggaagtg atggacgctg ttgcaccggg cggtctgggc ggcacctatg ccggtaaccc
     5101 gattgcctgc gtggctgcgc tggaagtgtt gaaggtgttc gagcatgaaa atctgctgca
     5161 aaaagccaac gatctggggc agaagctaaa agacggattg ctggcgatag ccgaaaaaca
     5221 cccggagatc ggcgacgtac gcgggctggg agcgatgatt gccatcgagc tgtttgaaga
     5281 tggcgatcac agcaaaccgg acgcaaaact caccgccgag atcgtggcac gcgcccgcga
     5341 taagggcctg attcttctct cctgcggccc gtattacaac gtgctgcgca tccttgtacc
     5401 gctcaccatt gaagacgctc agatccgtca gggtctggag atcatcagcc agtgttttgc
     5461 tgaggcgaag cagtagctat gtccgtttgt aagagttaac cgttgctaag ggaatgataa
     5521 cgatgcagta aaaaataata acgatgaggc gaacgacaaa gttaaaaacg actgcggaca
     5581 gtcccctcgc ccctctggag agagggttag ggtgaggggt aaaacacgcg gctaaaccct
     5641 cccctggccc tctccctaag ggagagggag aaaaccctcc cgctttcgtt tttaagattt
     5701 tgttgaccgt ctcaattaaa cgcgtgaccc ggcgcgggaa atgtcggggc gctctcccaa
     5761 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacat gagttaggcg
     5821 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     5881 tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc ggcggtatta ctggcctatc
     5941 tgttcgccgg gctactggtg gttatgatta tgcggatgtt ggcggaaatg gcggttgcca
     6001 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggtcgc tgggcgggct
     6061 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccgctg gaagccaata
     6121 tcgccgctat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     6181 tcattaccct tgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     6241 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     6301 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     6361 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     6421 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac acgccggaaa
     6481 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     6541 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     6601 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     6661 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     6721 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     6781 aaaccccgta cgtggcggtg ttactctcca ccggcgcggc attcttaacg gtggtggtga
     6841 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     6901 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcgg
     6961 aaggaagcga aattcgcttg cgaatgtggc tttatccctg gctcacctgg ctggtcatcg
     7021 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
     7081 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
     7141 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
     7201 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
     7261 cggtaatttt caaccggatg aaaaattacg gatgagtttg ctgacatcgc gttatgcact
     7321 tggcgttggg ccgttgcggg aagcactttc tcaactggtg gcggaacggc tggtcacggt
     7381 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
     7441 cgatgcccgc gccaatatgg aagcgatgtt agtgagtctg gcgattgccc gcggtggcga
     7501 tgagtgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
     7561 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
     7621 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
     7681 ggcgcgctat cgatttatct ggctgcggcg aacggtgctt tcggtagaaa tgctggagga
     7741 taaacacgat cagcaccaga ccctgactgc ggcggtactg gcgcgagata ccgcgcgcgc
     7801 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
     7861 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
     7921 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
     7981 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
     8041 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
     8101 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
     8161 tgccgtcggc aatttgaatg ttcactttat cagcatcagg tataccggtt ttgctcagat
     8221 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcgtcccaga
     8281 gtttttctcc ggcatctttc acaaaattga acagacccat agctacctca ttcgttaacg
     8341 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
     8401 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
     8461 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgag
     8521 cggcggtaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
     8581 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
     8641 gccagtttca gcttacgctt ttactctcat ttacgctgct gaggctggta tatgttgcat
     8701 ttttagttta gagcttactt aatttagaaa atacttaaat aaatatgact gaactcgcgc
     8761 aattacaggc cagtgccgaa caggcagcgg ccttattgaa agcaatgagc catcctaaac
     8821 gattgctgat tctgtgcatg cttagcggtt cccccggcac cagcgcggga gagctgacgc
     8881 gcattaccgg actgagtgcc tctgcgacat cacagcatct cgctcgtatg cgggacgaag
     8941 ggcttatcga cagccaacgg gatgcccaac gcattctata ttccattaaa aatgaggcgg
     9001 taaatgccat tatcgccacc ctgaaaaatg tctattgtcc gtaaggagtc actatggctt
     9061 tgacaaccat ttcgccgcat gatgcacaag aattaatcgc acgcggcgca aagttaatcg
     9121 atattcgtga cgctgatgaa tatcttcgtg aacatattcc tgaagcagat ctggctccat
     9181 tatccgtgct ggaacagtca ggtcttccac ctaaattacg tcgcgagcaa attatattcc
     9241 actgccaggc aggtaaacgc accagtaata acgccgataa attagcagcg attgccgccc
     9301 ccgcagaaat atttttactc gaagatggga ttgatggctg gaaaagagct ggattgccag
     9361 tagcggtaaa taaatctcaa cccttgccgt taatgcgcca ggtgcagatc gctgcgggtg
     9421 gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa tagcggtttc ttcttattaa
     9481 gtggctttgt tggtgccggg ttactgtttg caggaatcag cggtttttgt ggaatggcaa
     9541 ggttgttaga taagatgccg tggaaccaac gagcttgaga agcgacgccg gacgcgccct
     9601 agcagcgaca tccggcctca gtaattagat caggaaatcg tcgagagatt taccttctgc
     9661 cagcgcctga gcaattggct tcggtgtacg gccctgaccg gtccaggttt tagtttcacc
     9721 gttaacatcg gtgaatttat atttcgccgg acgcggctgg cgttttttac cagcgcgtgg
     9781 tgcagcagca gagctattac ccaataactc ttccgggtta attccgtcag ccttcatcag
     9841 ctccagccag gtgctaattt tttcctgacg ctcagccagt tcacgctgct gctgttcttc
     9901 ttcttcacgt ctttctttag tgacaaccct gaatttttcg agcatttctt caagaacgtc
     9961 aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg ttatttaaac tttgtaacat
    10021 tacggacata ataaagaaaa ccttttaacg ccaaaacaaa aaagtatttc gtcgcgtagt
    10081 gtaagcgatc acttattata tttccacaaa atttaatcta tttacagtct tgcgcgagat
    10141 tatttcagct gttattttct gaaactctca gaataatttc aagattatat attagccatt
    10201 ttgatgagta atttagatta ttggtgaaca gaacggcgct ctctgctcgc taattccgca
    10261 agcctgacaa aaccgaagtg aataaaaatg caatataaca gaataaaaga
//
LOCUS       DAZSBF010000144        10308 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZSBF010000144 DAZSBF010000000
VERSION     DAZSBF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.146"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            74..1468
                     /gene="galP"
                     /locus_tag="ACHXWD_002907"
     CDS             74..1468
                     /gene="galP"
                     /locus_tag="ACHXWD_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR7621770.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1545..2042
                     /locus_tag="ACHXWD_002908"
     CDS             1545..2042
                     /locus_tag="ACHXWD_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR7621771.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2137..2844
                     /gene="endA"
                     /locus_tag="ACHXWD_002909"
     CDS             2137..2844
                     /gene="endA"
                     /locus_tag="ACHXWD_002909"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR7621772.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2924..3655
                     /gene="rsmE"
                     /locus_tag="ACHXWD_002910"
     CDS             2924..3655
                     /gene="rsmE"
                     /locus_tag="ACHXWD_002910"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR7621773.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3668..4618
                     /gene="gshB"
                     /locus_tag="ACHXWD_002911"
     CDS             3668..4618
                     /gene="gshB"
                     /locus_tag="ACHXWD_002911"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR7621774.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4727..5290
                     /locus_tag="ACHXWD_002912"
     CDS             4727..5290
                     /locus_tag="ACHXWD_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR7621775.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5290..5706
                     /gene="ruvX"
                     /locus_tag="ACHXWD_002913"
     CDS             5290..5706
                     /gene="ruvX"
                     /locus_tag="ACHXWD_002913"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR7621776.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5881..6861)
                     /locus_tag="ACHXWD_002914"
     CDS             complement(5881..6861)
                     /locus_tag="ACHXWD_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR7621777.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            6879..7583
                     /gene="yggS"
                     /locus_tag="ACHXWD_002915"
     CDS             6879..7583
                     /gene="yggS"
                     /locus_tag="ACHXWD_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR7621778.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7601..8167
                     /gene="yggT"
                     /locus_tag="ACHXWD_002916"
     CDS             7601..8167
                     /gene="yggT"
                     /locus_tag="ACHXWD_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR7621779.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8164..8454
                     /gene="yggU"
                     /locus_tag="ACHXWD_002917"
     CDS             8164..8454
                     /gene="yggU"
                     /locus_tag="ACHXWD_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR7621780.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIVALIN"
     gene            8462..9055
                     /locus_tag="ACHXWD_002918"
     CDS             8462..9055
                     /locus_tag="ACHXWD_002918"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR7621781.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9048..10184
                     /gene="hemW"
                     /locus_tag="ACHXWD_002919"
     CDS             9048..10184
                     /gene="hemW"
                     /locus_tag="ACHXWD_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR7621782.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2412 a         2715 c         2732 g         2449 t
ORIGIN      
        1 ttgcttcaca tctttttacg tcgtactcac ctatcttaat tcacaataaa aaataaccat
       61 attggagggc atcatgcctg acgctaaaaa acaggggcgg tcaaacaagg caatgacgtt
      121 tttcgtctgc ttccttgccg ctctggcggg attactcttt ggcctggata tcggtgtaat
      181 tgctggcgca ctgccgttta ttgcagatga attccagatt acttcgcaca cgcaagaatg
      241 ggtcgtaagc tccatgatgt tcggtgcggc agtcggtgcg gtgggcagcg gctggctctc
      301 ctttaaactc gggcgcaaaa agagcctgat gatcggcgca attttgtttg ttgccggttc
      361 gctgttctct gcggctgcgc caaacgttga agtactgatt ctttcccgcg ttctactggg
      421 gctggcggtg ggtgtggcct cttataccgc accgctgtac ctctctgaaa ttgcgccgga
      481 aaaaattcgt ggcagtatga tctcgatgta tcagttgatg atcactatcg ggatcctcgg
      541 tgcttatctt tctgataccg ccttcagcta caccggtgca tggcgctgga tgctgggtgt
      601 gattatcatc ccggcaattt tgctgctgat tggtgtcttc ttcctgccag acagcccacg
      661 ttggtttgcc gccaaacgcc gttttgttga tgccgaacgc gtgctgctac gcctgcgtga
      721 caccagcgcg gaagcgaaac gcgaactgga tgaaatccgt gaaagtttgc aggttaaaca
      781 gagtggctgg gcgctgttta aagagaacag caacttccgc cgcgcggtgt tccttggcgt
      841 actgttgcag gtaatgcagc aattcaccgg gatgaacgtc atcatgtatt acgcgccgaa
      901 aatcttcgaa ctggcgggtt ataccaacac cacagagcaa atgtggggga cagtgattgt
      961 cggcctgacc aacgtacttg ccacctttat cgcaatcggc cttgttgacc gctggggacg
     1021 aaaaccaacg ctaacgctgg gcttcctggt gatggctgct ggcatgggcg tactcggtac
     1081 aatgatgcat atcggtatcc actctccgtc ggcgcagtat ttcgccatcg ccatgctgct
     1141 gatgtttatt gtcggttttg ccatgagtgc cggtccgctg atttgggtac tgtgctccga
     1201 aattcagccg ctgaaaggcc gcgattttgg catcacctgc tccactgcca ccaactggat
     1261 tgccaacatg atcgttggcg caacgttcct gaccatgctc aacacgctgg gtaacgccaa
     1321 caccttctgg gtgtatgcgg ctctgaacgt actgtttatc ctgctgacat tgtggctggt
     1381 accggaaacc aaacacgttt cgctggaaca tattgaacgt aacctgatga aaggtcgtaa
     1441 actgcgcgaa atcggcgctc acgattaatc tcctcaggct tcctcccgtc gcggaggaag
     1501 ccgcctcttg cagtcatctt ttcttcgctc tatcctctgc cgctatgaaa acatcccgtc
     1561 tccctatcgc catccaacag gccgttatgc gcggcctgcg ggaaaaactc gcccaggcca
     1621 acctgaagct agggcgtaac tacccggagc caaaactctc ttacacccag cgcggaacct
     1681 ccgccggaac agcctggctg gaaagctatg aaatccgcct caatcccgtt ttgctgttgg
     1741 aaaacagtga agcttttatt gaagaagtgg taccgcacga actggcacat ttgctggtat
     1801 ggaaacattt cggccgcgta gcgccacatg gcaaagagtg gaagtggatg atggaaagcg
     1861 tgctgggtgt tcccgcccgt cgtacgcatc agttcgaact gcaatccgtg cgtcgcaaca
     1921 ccttccccta ccgctgcaag tgccaggagc atcagcttac cgtacgccgc cataatcgcg
     1981 tagttcgtgg cgaggccgtc tatcgctgtg ttcactgcgg tgaacagctg gttgcgaaat
     2041 aaccatctga actatcagga actttcctga tctggctgat tgcataccaa aacaactttc
     2101 gctacgttgc tggctcgttt taacacggag taagtgatgt accgttattt gtctattgct
     2161 gcggtggtac tgagcgcagc attttccggc ccggcgttgg ccgaaggtat caatagtttt
     2221 tctcaggcga aagccgcggc ggtaaaagtc cacgctgacg cgcccggtac gttttattgc
     2281 ggatgtaaaa ttaactggca gggcaaaaaa ggcgttgttg atctgcaatc gtgcggctat
     2341 caggtgcgca aaaatgaaaa ccgagccagc cgcgtagagt gggaacacgt cgttcccgcc
     2401 tggcagttcg gtcaccagcg ccagtgctgg caggacggtg gacgtaaaaa ctgcgctaaa
     2461 gatccggtct atcgcaagat ggaaagcgat atgtataacc tgcagccgtc agtcggtgag
     2521 gtgaatggcg atcgcggcaa ctttatgtac agccagtgga atggcggcga aggccagtac
     2581 ggtcaatgcg ccatgaaggt cgatttcaaa gaaaaagctg ccgaaccacc agcgcgtgca
     2641 cgcggtgcca ttgcgcgcac ctacttctat atgcgcgacc aatacaacct gacactctct
     2701 cgccagcaaa cgcagctgtt caacgcatgg aacaagatgt atccggttac cgactgggag
     2761 tgcgagcgcg atgaacgcat cgcgaaggtg caaggcaatc ataacccgta tgtgcaacgc
     2821 gcttgccagg cgcgaaagag ctaacctaca ctagcgggat tctttttgtt aacccctacc
     2881 ccacgcgtac aaccgcgtgg ggagacgacg cggattttta actatgcgta tcccccgcat
     2941 ttatcatcct gaaccactga ccagccattc tcacatcgcg ctttgcgaag atgccgccaa
     3001 ccatatcggg cgcgtactgc gcatggggcc ggggcaggcg ttgcaattgt ttgacggtag
     3061 caaccaggtc tttgacgccg aaattaccag cgccagcaaa aaaagcgtgg aagtgaaggt
     3121 gctggaaggc aagatcgacg atcgcgaatc tccgctgcat attcacctcg gtcaggtgat
     3181 gtcgcgtggt gaaaaaatgg aatttacgat ccagaaatcg atcgaactcg gtgtaagcct
     3241 cattacgcca cttttttctg agcgctgcgg cgttaaactg gatagtgaac gtctgaacaa
     3301 gaagctccag cagtggcaga agattgcaat tgctgcctgt gagcagtgtg gtcgtaaccg
     3361 ggtgccggaa atccgtccag cgatggatct ggaagcctgg tgtgcagagc aggatgaagg
     3421 actgaaactg aatcttcacc cgcgcgccag taacagcatc aatacgttgc cgttaccggt
     3481 tgaacgcgtc cgcctgctga ttggcccgga aggcggttta tcggcagatg aaattgccat
     3541 gactgcccgc tatcaattta ctgatatcct gttgggacct cgcgttttgc gtacagagac
     3601 aactgcgctc accgccatta ccgcgctaca agtacgattt ggcgatttgg gctaacggag
     3661 aagaataatg atcaagctcg gcatcgtgat ggaccccatc gcaaacatca acatcaagaa
     3721 agattccagt ttcgctatgt tgctggaagc acagcgtcgt ggttacgaac ttcactatat
     3781 ggagatgggc gatctgtatc tgatcaatgg cgaagcccgc gcccataccc gcacgctgaa
     3841 cgtgaagcag aactacgaag agtggttttc gttcgtcggt gaacaggatc tgccgctggc
     3901 cgatctcgat gtgatcctga tgcgtaaaga cccgccgttt gataccgagt ttatctacgc
     3961 gacctatatt ctggaacgtg ccgaagagaa agggacgctg atcgttaaca agccgcagag
     4021 cctgcgcgac tgtaacgaga aactgtttac cgcctggttc tctgacttaa cgccagaaac
     4081 gctggttacg cgcaataaag cgcagctaaa agcgttctgg gagaaacaca gcgacatcat
     4141 tcttaagccg ctggacggta tgggcggcgc gtcgattttc cgcgtgaaag aaggcgatcc
     4201 aaacctcggc gtgattgccg aaaccctgac tgagaatggc actcgctact gcatggcaca
     4261 aaattacctg ccagccatta aagatggcga caaacgcgtg ctggtggtgg atggcgagcc
     4321 ggtaccgtac tgcctggcgc gtattccgca ggggggcgaa acccgtggca atctggctgc
     4381 cggtggtcgc ggtgaacctc gtccgctgac ggaaagtgac tggaaaatcg cccgtcagat
     4441 cgggccgacg ctgaaagaaa aagggctgat ttttgttggt ctggatatca tcggcgaccg
     4501 tctgactgaa attaacgtca ccagcccaac ctgtattcgt gagattgaag cagagtttcc
     4561 ggtgtcgatc accggaatgt taatggatgc catcgaagca cgtttacagc agcagtaacc
     4621 caccttagcg agaaggatct cgttgagact ctgagtgaca gcgcccttct ttccacgcat
     4681 actgggcgct gttgcttttt tgaaccagga aacagaacct ctgacaatga atttacagca
     4741 tcactttctt attgccatgc ctgctctcca ggatccgatt ttccgtcgtt ccgtggtcta
     4801 catttgcgaa cataatacca atggtgcaat ggggatcatc gtcaacaagc cgctggaaaa
     4861 tctcaaaatt gaagggattc tggaaaagct gaagatcacg ccggagccgc gtgatgaatc
     4921 aatccgtctg gataaaccgg ttatgctcgg cggtccgctg gctgaagatc gcgggtttat
     4981 tttgcatact ccgccctcca attttgcttc cagcattcgc atttcagaca acacagtgat
     5041 gaccacttcc cgcgatgtgc tggaaacgct cggcaccgat aaacaaccgt ctgacgtatt
     5101 ggtggctcta ggttatgcct cctgggaaaa aggtcaactg gaacaagaaa ttctcgataa
     5161 cgcgtggcta acggccccgg cggatctgaa tattctgttc aaaacgccga ttgccgaccg
     5221 ctggcgcgag gcggcaaaac tgattggtgt ggatattctc accatgcctg gtgtggcagg
     5281 gcacgcctga tgagtggaac cttactcgcc ttcgacttcg gcaccaaaag cattggcgta
     5341 gcggtcggcc aacgcattac cggcactgct cgccctttgc ctgcaattaa agcacaggac
     5401 ggtacgccgg actggaacct catcgaacgt ttactgaaag agtggcagcc ggacgaaatc
     5461 atcgtcggtt tgccgctgaa tatggacggc accgagcaac cattgactgc cagagcgcgt
     5521 aaatttgcca accgtattca tggtcgtttc ggtgttgaag taaagctcca tgacgaacgt
     5581 cttagcaccg tggaagcccg ttccggtctg tttgaacagg gcggctatcg ggcgctcaac
     5641 aaaggcaaag tcgactctgc ctctgcggtt attattctcg aaagctattt cgagcaggga
     5701 tattaaggcg atttaaaagc ctggcggata gtcgttaccg ccatcttgcc ggatgcggcg
     5761 tgaacgccta tccggcctac atgtggcagc gattgtaggt ctgataagac gcgcaaacgt
     5821 ctcatcagac gtttattgcc tttgtaggcc cgataagctt gcacatcggg catggcaacg
     5881 tcacaaacgc ccttccccca ctcgctgctg aaaactctgc tgaaacgtta tcatccccac
     5941 ctgctgcccg gtttgaataa catgcggtaa ctggtgggtt ttcccttcgc gaatcaaatt
     6001 ccccaccgcg ggagtgttaa tcaataattc aaacagcgcc acgcgtcctt cctgtttatc
     6061 cacttccagc ttttgtgaca acacggcccg taaactacct gccagttgat tacgtacggg
     6121 atctttttcc tgcgccggaa atgaatccac cagtcgctca actgcctgcg cggcaccacg
     6181 cgtatgtaat gttgccagca ccaaatgccc ggtttctgcc gccgtcagtg ccagacggat
     6241 tgtctcgctg tcacgcagct ctccgagcaa aatcacatca ggatcttccc gcaatgcggc
     6301 ccgcaatccc gatgcgaacg tcatacagtg caaaccaatt tcccgcttct ggatcaaaca
     6361 tcgctggctg gcatagatat attcaacagg atcttccagc gtcagaatat gcgcatcggc
     6421 atgttgattg agatagccaa ccatcgccgc cagcgtggta gatttgctgc tccccgtcgc
     6481 ccccgtcacc agaatcaggc cattctcact cttgagtaat tccggcaata ccgttggtgc
     6541 gccaagctgt tcgagctgcg ggcagtgcga aggtaacagc cgtaacgcca gcgaaatgcc
     6601 atgccgttgc gcaaatgcgc tgccacgcaa tcgctggttt tccgccagcg acacggcaaa
     6661 atccagctgg ccattttcca gcaatattgc ccgctgatcg tcatccagcc actcccgcag
     6721 tagctcttcg acgtccggcg cctcaaacgg cgcagcttcc attagcccgc gaatgcgcca
     6781 tcgtgcgggc caggcgctgc acaggtgtag atccgagacg ttatgcttta cactaagggc
     6841 cacaatttct tccatattca tactaagatc ctcggaaaat gaacgatatt gcgcataacc
     6901 tggcacaggt ccgggacaaa atctcagcgg ctgcaacgcg ttgcggccgt tctccagaag
     6961 aaattacgct gcttgcagtc agtaaaacaa aacctgcgag cgccatcgca gaagccattg
     7021 atgccgggca gcgtcaattt ggtgaaaact acgttcagga aggggtagat aaaattcgcc
     7081 actttcagga actgggcgta acaggattag aatggcattt tattggcccg ttgcagtcta
     7141 ataaaagccg cctggtggca gagcatttcg actggtgtca taccatcgac cgtttgcgca
     7201 ttgctacccg cctcaacgat cagcgcccgg cagaacttcc ccctcttaac gtactgattc
     7261 aaattaatat tagtgatgaa aacagtaagt ccgggattca actggcagaa ctggacgagc
     7321 tggcagcggc ggtcgctgaa ctaccgcgtt tacgtctgcg cgggctgatg gcaatccctg
     7381 cgcctgagtc agaatatgta aggcagtttg aagttgcacg ccaaatggct gtagcatttg
     7441 ccggactgaa aacgcgctac ccgcatatcg acacgctctc tctgggaatg tcggacgata
     7501 tggaagccgc cattgcggca ggtagcacga tggttcgtat cggcactgca atttttggtg
     7561 cgcgtgatta ctctaaaaaa taaggaatta aaggaacgcc atgaatacgt tgactttcct
     7621 gctttcaacg gtcattgagc tgtataccat ggtgctgtta ttacgcatct ggatgcagtg
     7681 ggctcattgt gatttttaca accccttctc acagtttgta gtgaaggtaa cgcagccaat
     7741 tatcgggcca ctgcgccgcg ttattccggc aatgggcccc attgacagcg cctcgctgct
     7801 ggttgcctat attctcagtt ttatcaaagc catcgtgctg tttaaagtgg tgaccttcct
     7861 gccaatcatc tggattgctg gtttactgat tctgctgaaa accatcggcc tgctgatttt
     7921 ctgggtcctg ctggtgatgg cgattatgag ctgggtaagc cagggtcgta gtccgattga
     7981 atacgtgctg attcagctgg ccgatccgct gctgcgcccg attcgccgcc tgctaccggc
     8041 aatgggtggg attgatttct cgccgatgat cctcgttctg ctgctgtatg tcatcaatat
     8101 gggtgtcgca gaagtattac aggcaaccgg aaacatgctg ctgccggggc tgtggatggc
     8161 gttatgagtg ccgtaacagt taatgatgac ggtctggttt tacggctcta tattcagccg
     8221 aaagccagcc gtgattctat tgtcggttta catggcgacg aagttaaagt cgccattacc
     8281 gcgccgccgg ttgacggcca gggcaacagt catctggtga agtttctcgg taagcaattc
     8341 cgggttgcca aaagccaggt ggtgattgaa aaaggcgaac ttggccgcca caaacaaatt
     8401 aaaatcatta atccgcaaca aatcccgcca gaaatcgtgg cgttaattaa ttaggtatcc
     8461 tatgcaaaaa gttgtcctcg caaccggcaa tgccggtaaa gtgcgtgagc tggcgtcgct
     8521 gcttagcgac ttcggtcttg atatcgtggc ccaaacagac ctcggcgtag attccgctga
     8581 agaaaccggc ctgaccttta tcgaaaacgc gattctgaaa gcgcgctatg cggcaaaagt
     8641 gaccggttta ccggcaattg ccgacgactc tggtctggcg gtagatgcgc tgggtggcgc
     8701 gccgggtatt tactccgcgc gttattccgg tgaagacgcg accgatcaaa agaatctgca
     8761 aaaactgttg gaaacactga aagacgtacc ggacgaccaa cgtcaggcgc gtttccactg
     8821 cgtgctggta tatctgcgtc acgcggaaga tccgactccg ctggtatgcc acggcagctg
     8881 gccgggcgtg attactcgcg aaccagcggg cactggtggc tttggttatg atccaatctt
     8941 cttcgtacct tccgaaggga aaaccgccgc cgaactgacc cgcgaagaaa agagcgccat
     9001 ttcccaccgt ggtcaggcgt tgaaactgct gctggacgct ttacgtaatg gttaaattac
     9061 cgccgctgag tctctacatt cacatcccgt ggtgcgtgca gaaatgcccg tactgcgatt
     9121 tcaactctca cgcgttgaaa ggagaagtgc cgcacgacga ttatgttcag catctgcttt
     9181 gcgatctgga caatgatgtg gcttacgctc agggccgtga agtaaagaca atctttattg
     9241 gcggtggtac gccgagcctg ctttccggcc cggcgatgca aacgctgctg gacggcgtgc
     9301 gtgcgcgttt gccgctggca gcggatgcag aaattactat ggaagcgaac cctggaacgg
     9361 tagaagccga tcgctttgtc gattatcagc gtgctggtgt gaaccgcatc tctattggtg
     9421 tgcagagttt tagcgaagaa aagctgaaac gacttgggcg tattcatggc ccgcaagaag
     9481 cgaaacgcgc ggcaaagctg gcgagcgggc tggggctgcg tagctttaac ctcgatttga
     9541 tgcatggact accggatcaa tcactggaag aggcgcttgg cgatctacgc caggccattg
     9601 aactgaatcc gccgcatctt tcctggtatc aactgaccat cgaacccaat acgctgtttg
     9661 gttcgcgacc accggtgctg ccggacgatg acgcgctgtg ggatattttc gaacaggggc
     9721 atcagttatt aaccgcagcg ggttatcagc aatatgaaac ttccgcttac gccaaacccg
     9781 gttatcagtg ccagcacaat ctcaactact ggcgatttgg tgactacatc ggtattggct
     9841 gcggcgcgca cggcaaagtc accttcccgg atgggcgcat tctgcgtacc actaaaacgc
     9901 gtcatcctcg tggttttatg caaggaaggt atctggaaag ccagcgtgat gtcgaagccg
     9961 cagataagcc gtttgagttc tttatgaatc gcttccgtct gctggaggcc gcgccgcgcg
    10021 tggagtttat tgcgtatacc gggctttgcg aagatgtgat tcgcccacag ttagacgagg
    10081 cgattgccca gggttatctc accgaatgtg cggattactg gcagataacc gagcacggta
    10141 aattattctt aaactcactt cttgaattat tcctcgctga gtaatcatta atgcgcctgc
    10201 gggcgcatta atcacttctt tcccccccgt taattaactt cggcaaaata tatttcttac
    10261 tgaataaaca tcgatgatat ttattagata tatctagtga atatttac
//
LOCUS       DAZSBF010000145        10284 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZSBF010000145 DAZSBF010000000
VERSION     DAZSBF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.147"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..357
                     /locus_tag="ACHXWD_002920"
                     /pseudo
     CDS             1..357
                     /locus_tag="ACHXWD_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(443..1006)
                     /locus_tag="ACHXWD_002921"
     CDS             complement(443..1006)
                     /locus_tag="ACHXWD_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR7621783.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREVLPCGTFAYTRVPTMIIRLRA"
     gene            complement(1053..2414)
                     /locus_tag="ACHXWD_002922"
     CDS             complement(1053..2414)
                     /locus_tag="ACHXWD_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFR7621784.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2466..2696)
                     /locus_tag="ACHXWD_002923"
     CDS             complement(2466..2696)
                     /locus_tag="ACHXWD_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621785.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPTPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(2920..3039)
                     /locus_tag="ACHXWD_002924"
                     /pseudo
     CDS             complement(2920..3039)
                     /locus_tag="ACHXWD_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332787.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(3734..3925)
                     /locus_tag="ACHXWD_002925"
     CDS             complement(3734..3925)
                     /locus_tag="ACHXWD_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621786.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3922..4344)
                     /locus_tag="ACHXWD_002926"
     CDS             complement(3922..4344)
                     /locus_tag="ACHXWD_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR7621787.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4391..4816)
                     /locus_tag="ACHXWD_002927"
     CDS             complement(4391..4816)
                     /locus_tag="ACHXWD_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR7621788.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5234..6010)
                     /locus_tag="ACHXWD_002928"
     CDS             complement(5234..6010)
                     /locus_tag="ACHXWD_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621789.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(6061..6495)
                     /locus_tag="ACHXWD_002929"
     CDS             complement(6061..6495)
                     /locus_tag="ACHXWD_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFR7621790.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6509..6730)
                     /locus_tag="ACHXWD_002930"
     CDS             complement(6509..6730)
                     /locus_tag="ACHXWD_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621791.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6731..7414)
                     /locus_tag="ACHXWD_002931"
     CDS             complement(6731..7414)
                     /locus_tag="ACHXWD_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR7621792.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7491..7784)
                     /locus_tag="ACHXWD_002932"
     CDS             complement(7491..7784)
                     /locus_tag="ACHXWD_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621793.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7931..8893
                     /gene="parM"
                     /locus_tag="ACHXWD_002933"
                     /gene_synonym="stbA"
     CDS             7931..8893
                     /gene="parM"
                     /locus_tag="ACHXWD_002933"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR7621794.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8896..9246
                     /locus_tag="ACHXWD_002934"
     CDS             8896..9246
                     /locus_tag="ACHXWD_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR7621795.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9369..9650)
                     /locus_tag="ACHXWD_002935"
     CDS             complement(9369..9650)
                     /locus_tag="ACHXWD_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621796.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9778..10012)
                     /locus_tag="ACHXWD_002936"
                     /pseudo
     CDS             complement(9778..10012)
                     /locus_tag="ACHXWD_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10023..>10284)
                     /locus_tag="ACHXWD_002937"
     CDS             complement(10023..>10284)
                     /locus_tag="ACHXWD_002937"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621797.1"
                     /translation="NGNGCANLYLEVLLQGTSTPSLHQYRIAPDTRHPDINLIKAHLD
                     EGFQQAKSEGLKVEISDYKERLYLYIRTPGNNLMQYSGCREK"
BASE COUNT         2422 a         2866 c         2668 g         2328 t
ORIGIN      
        1 gtcttttgcc gggatgccgg gagcgcaggc cacggcgcga caagggtggc gcgaccgttc
       61 cggtcgctgc aaacccgggt ttttgcgtca gggatggaag cccgccaggg cggagacagc
      121 tccgctggct ccgttcacga cagcccgccc cgccaggggg acgcccggaa aaggcataag
      181 caggagaaca acaaaacaga cagtaatcag agcagcagaa aaaagaagaa cagtagcagt
      241 agcagtagca gtagcagtag caggaacaac aacatcatca gtaacaggca aatgacaggt
      301 ctgaaaagac agaagcccac agcagaacgt ccgcgtaatg aaaggggcgg cgcgtaagcg
      361 cggccccttt cagcgccacc ggcgcgcggt cttttcccgg cgcgggcaga cgggcattta
      421 tcctcccgcc tgcccggatt catcaggcac gcagacgaat aatcatcgtc ggaacacggg
      481 tataagcaaa tgtgccgcac ggcagcacct cgcggacatc agaaaacggt aacagcttct
      541 cctgctggcg cggcccgttc agacagacgg caaccagaac gccccccgga cgcaacaggg
      601 aaaaagcccg caggatatgc cggatatcct gcccgtgact gaacggcgga ttcataatga
      661 tccggctgta atactgcacc ggctgccatt ccatgaagtc gccacactgt accctgacac
      721 cgttaaagtt ttcccgcaga taccggacca gcccgctgtt aatctccacc gcatcacaca
      781 tggcctccgg cgcggtatcc cgaatcgccc gcagtatggc cccggtgccg gcagagggtt
      841 ccagaatgtg atcacggttg ctgatttccg ccagcgtcac caggcgatca cagaccgggg
      901 caggagtcac aaaaagctga gattcagccc ggatttcagc aaccggcgcg gcgttatcca
      961 gccttgcgcg catcagccgg atacgttctc ttaactgact gtgcatgtca ttatctccgt
     1021 catcccccgg ggatttcccg gggggatata ccttatttct gcgggatttc cgtgattttc
     1081 atgtcggtga tatacacatt caccagacgg aaattattgt ccattgtgcg gcggtagcgg
     1141 tatgccccgt ggtcttctgc ttcttccaca ctgcgcacgg ccttacagtc ccggggcagg
     1201 gcggcccact gcgcctttgt catttcccgg aaaccttccc ccggatagtt gactaccggc
     1261 ggacgcttcg cggcctgact ggcgacggca gcctcttctg ccgatggtgc tttgtagtcc
     1321 gtgatgcgat cgggcgtcac tttcatcgtg ccgctgtacc cgagaaaact gtaatttggc
     1381 gttgtgactg aactcaccgc cccgttgctt ttgttcacgc ggatgatagt cagccactcg
     1441 ccccggctga aaacctgtcc gcccggctca aaatcctgtg tccgggtaac cacgccgccg
     1501 ctttcgtcca gcatggcacg ctcatagatc aggcggttct gatagtggtt aacccagcgt
     1561 tgctgatgct gaatctgccg ttcatgacag cggatcgcaa tttcgcgggc ctgctccgtg
     1621 gtgatgatgt catcatccag ggccgaccat aacgaccggc tcccttcata ctggcttttt
     1681 tctgccgggc gcggatacgt atccagcggg aaacaggcgc tgatatggtc atgactgctg
     1741 atgagttttg ccatattcag atccagcgat tcagcccgcc acatcgtcag atatttctgc
     1801 gactgtgcga tggtcttttc agccttgcgc aaatccgctt cgattttttt gatacgacgc
     1861 cagcgaacgt ccgggcgttc tttatacttc gcgtgaagca gtgccgaccg cgcccgctcc
     1921 tcccagtatt ccgcacgttc aaagagcatc acggcacgtt tcatgccgtt ttcaatacgc
     1981 tgcgcatcac gacgggcgcg gcgttcgctg tgatgcccca caagaatggg ctgaccgggc
     2041 gggatcatcg cggccagtct ttccacttca tcaagtgcct gtgcggattc actggcccgc
     2101 tttccgctgt atccggtaaa ccgctccgcc cgtgcttcct gacgttcagc gagcgtgctg
     2161 tcttcatcct caatctctcc ggcaagtgag aggagcacgt cttcccggcc cggtgtccag
     2221 gccggcgcga caaacagcgc ctgtttgggt gcccagcgaa aaccggcatc atgcactttt
     2281 ttatacgtct ccgggtcaag acgtgacgcg gcatacaggc gcagtttgtt gtcatccggt
     2341 gagtaggtcg cgcggtacat ctgcacaggg ctggcggctt ccggggcggt catggtgatt
     2401 gctgctaatg tcataaaatg ttctccttcc gtctgttgcc gcccctcgcg gggcggctca
     2461 cctcatcagt taaaagcgcg tttcagttcg agtgtgcgga gcacctgccc gaaagccgtg
     2521 tttttcatgg cggggatgtg tacaccacgc ccccggtgaa aactccgggc tatgtgtcgc
     2581 ccgaaggctt tcaacacggg gtcaatttcc ggcgtgggga tgtcttcgcg tctgaaacgg
     2641 ggatgacggc ggggctttac agccgctgcg gaaggtgaaa caatgcttac agtcataaat
     2701 ggattcctta tgacaaattg gttaacaatg ccgtcaaaat tggtcgcaca attcttacgg
     2761 cgtggattaa gcaggtgcct gacctgcttt tttcatatct gcatctgacg ctttccggtg
     2821 ttttcctcct tctgttcctg cgttctcatt acctgtcagc cggatgactg acagaacaaa
     2881 taaatgcgta ttgccggagc ctgaaataaa tcctgatttt taaagaacat cacccgttga
     2941 gtttcgggag ccgcggtttt gacgtttcgc gggaacctgc tgctgtattc actgtggtat
     3001 gaacttcacc cgtttgagaa ccttcgccgc aaggggcatc actgccacgg cctgacgcac
     3061 ctgtcctggg cagaacgcaa aattttttac cgggttttgg gtaaaaaatt ttgtgggctg
     3121 ttcatttacc ctggacagtc agtgcggcag gttgtggctg tatcagcccc cggcgacagg
     3181 gactcatacg ggtgtgtggc acccggcagg caatgagcag gggcccgccg caacgacgga
     3241 gagcgcgtgc gcgaaacggc gttgcccctt acatcccggc ggccttttgc cgggatgccg
     3301 ggagcgcagg ccacggcgcg acagaggcgg cgcgaccgga acggtcgctg caagccaggg
     3361 gttttcacgc atcggaccgt agcggcagcc cgaaaccgcg ccagcgggtt cggcggagac
     3421 tggcaggtgc gccagcagct gcctgtcgga gtcgggcctg acgaccggat gccgccaggc
     3481 agccgggagc cgaaggtgcg cccggcagac agaacaacac tgcagtagtc agaatcagag
     3541 cagtagataa aggaacaaca acatcaggaa cagttccata acataagaaa gaaacactcc
     3601 tgagtcagac acatcacaga cgggaagggt aacgggcgac gcgccagcgt ggcccgcttc
     3661 ccttccggca ggcgtcagcc tgccaggggt tttcgcgcgg gctgacaggc ggcctccacc
     3721 gcctgcccct gtatcacctg tttatgctgc cctgaaggat gtagtgaccg ccgaggaata
     3781 cagcctgctg attcgccaca aaatcgcaga tttcatcaac aatctgcgca gtggtgagcg
     3841 gtttctgccc catttcacga cgccgcgcat taatcacggc tttgtaattg cgcagaattt
     3901 cgcgggtttc tgtgctgata ttcatgacat gtgctccccg tccagcgcct gtaacacaac
     3961 gtccattgct tcgcgttctt ccctgacgaa tgcgtcaccg tcgcccccgt tctcgctccc
     4021 gacggcatac aggcgcttca tttcactgcc tgccagcgcc atcacttttt ccagcatcac
     4081 cgccggaact gtcacctgac ggctggcgcg cttttcttcc atcagctcac gaacaacgtc
     4141 gtgaacaatg ctgacatccg caccatgcgc aaaggcatcg tccagtcgct gcatgacggt
     4201 ttccagctca tcgtctgtca gactccagtc ctgggctatg tgacgcacat ccccacgggt
     4261 cagcaccacg gcaaaaacgc attcactgtc cggcacgtct gtgttcagca cttcgcggat
     4321 aatttcttta acagtacagt acatgttctc gtctccttta caggggcggc attgccgccc
     4381 cgtccgttaa tcagtccagt gccgcgtaaa tcgcgttgca ttcggggtga aattcgatgt
     4441 gacgccacaa ctgcgcatcg cgcatccggt aaagctgggt cagtcctgca tccccgctgt
     4501 cgtggtacag ccacaactgg cggttaatca caagggaagt cagaatgatg cctgcggcat
     4561 cagcactcac ggtacggtca aaccagtttg caccgttcac catgtggaaa cggtcgccat
     4621 ccggcatcat gtaaccgcca ccgtccggca ggcggatgaa gtgccagaag cctccctgat
     4681 attccggcat cattttttgc gccagcgcgt acacatacgc ctcggcatac agaaaatcgt
     4741 tgccaaacag atacggtaga aaggagagac ggtcgcactc ttcaacgttc agagtgacag
     4801 gtttcgcata ttgcatgatg ttttcctccg ttgttcacat attccgcgac accgggcggt
     4861 gccgtgcctc cataaaagcc gggcgataag gcggggatgc acagggcgca agggaagccg
     4921 cagcctgacg cacacggctg gcgggagttg gagggtgaga ccgcgaaatc tgagcgggtg
     4981 aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgaccgcag cggcgaagcg
     5041 tacccttgca tccctgccag cccggctaag atggaggcac ggcacagccc gcacacgacg
     5101 ctgaggccac ataactgaaa aaccacaagg ccatgccctg catggccggg taaagcggag
     5161 ccggcggcga cttcctttgt gctctgtccg gggaccggca ggaaaaaggc ggggatttac
     5221 cccgccaacg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     5281 cccgggcttc ccggaccacc tcccgcagaa caccacggtc aaaccagcgg cggggcggtt
     5341 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     5401 cgctgaccag gtacatttcc accgacgaaa aacgtgctcc ccggttcacg atggcatcga
     5461 gcaccacggg caggttttcg tcatcttcat cgagccagca ggggtgatcg ctttccttca
     5521 caaccagacg aaagcggcgc gtctggcgag tcagcaacgt ctgaccggtg gtggtcagcg
     5581 ccgcccggat acgccgcgca tcttctgcca cccggcaact gacactgcgt accgcatcgc
     5641 gcaccgccgc aagtacagcc ctgatttcgt cttcggtcag ccattctccg gcatacggga
     5701 aagacacctg cgttccgtct gccggaagta ccggcagcga ctgccactga ctgccggaca
     5761 gtaccagttc cgccccggtt ccggtcagtt tctgaatggc ggaggccagg agcggcggca
     5821 gtacgccgcc gtgacggttg ataagtaacg tcgccagccg gggcgcatcc agcagaacat
     5881 aaatgctgat ccagcccgga tacttcgggt tacgctggtc aacatccgtc tcttccggcc
     5941 agccctgcgc cagcagcagg ttgcgggcgt gacgacaaat cagtgcgttc agcgtgctgt
     6001 tttcgttcat gattaagcgt tctccctgta tcgccgcccg cagggcaggc ggcgataaca
     6061 tcagtcgttt tcgtgtgtgt tgtttttcag cagggtgcgg gcctgattaa ccacctcctg
     6121 acggcgggtg acgcattccg ggacggcgag gccatgatcc cgggcggccc attcacgttt
     6181 ccacaatgcc tgttcagcgg tctgaatcag ggacgccagc agttcagcag ggacggtcac
     6241 caggcggttt tcgctctcgt tgcggataat ctccatcacc gcagcggaag aaataccgga
     6301 ctcaatccgc tggtcttccg ggatgtcctc caggcgtgcc aggacggtcc tgatttcatg
     6361 accggtcaga gaaatttcca taccgtcagc cagtgcctga atctcttccg gggaccagat
     6421 aaccagcatc agcggcatgt ctcccggata ctttactgcc agctcccggc atagcgtttc
     6481 gcatgttccg tacattctcg tctccttctc aatctttaaa gcacgcttcc agttccagaa
     6541 tttcggtata tgcctccatc aggaggcggt ttgcgcgaaa cagcgtctga cgcaccagac
     6601 gttccggcag ggtgtcggca ttaaagctgt accgctgctc catctcattc atggttgtac
     6661 gcaggtcgca catggcattg gccacctccg ccacttcggc gcgcagtttt tcgtgtcctg
     6721 catagttcat ttacgcagcc tccggcataa accagtcatc attcgcggcc ccctgctgca
     6781 tggcccgttg cacggcggca aggcgttgct gcccggcacg gtgatactgc tcaagcagct
     6841 cgataccgat ataccggcgt ccggactgga gggcggcgac gcaggttgag ccgctgcctg
     6901 caaacgggtc cagcacaatt gcgttcgggt gtgtgaagct ctcaatcagc ggttgcaggc
     6961 tggtaacggg cttttccgtc gggtgatggc gattgcccga atatttccag cccagcacgt
     7021 ccggcagcgg gttttgtggc agacgtggac ggccttttgc caggatgtag gcacattcgt
     7081 ggcgatagcc cacatatgcg gccttcgatg tgtagttttt ggtgaagacc aggtgaccaa
     7141 caacgctgaa tcccgcattt ttccaggcgg ccataaagcg atcgacgcgg ttccagccat
     7201 agaagctcac cattaacgcg tcttttttga gtacgcggta catttcatta caggctggtt
     7261 gcagccattc atcggtttta tcgccggcga tggtgcgccc ggaacggtca cggaaaccga
     7321 cgagatacgg cgggtcggtg aggatgaaat cgacagcatt gcccggaaac gtggccataa
     7381 cacgcacgca gtcacccagg ataaaacggg acataacagc actccttctc ttacagaggg
     7441 caccgggagg gcacccctgt ggtgcccgtt ccccgctgcc tgtgggctgt tcaccgcgtc
     7501 cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctttttga cggcgaggat
     7561 atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacgggct ggcgtacact
     7621 gtcaccaacg ccagccggga gcggtaaaag cgcccgtccc gtgcccggcc ggacgcggga
     7681 cactgaccac aggcagcgtt cctgccgcgc cgcgtcagcc ggcgcagtaa aaagaggagc
     7741 ccggcgacat cttttgttac ccgccaaaca aaacccaaaa acaacccata cccaacccaa
     7801 taaaacacca aaacaagaca aataatcatt gattgatggt tgaaatgggg taaacttgac
     7861 aaacaaaccc acttaaaacc caaaacatac ccaaacacac accaaaaaaa caccataagg
     7921 agttttataa atgttggtat tcattgatga cggttcaaca aacatcaaac tacagtggca
     7981 ggaaagcgac ggaacaatta aacagcacat tagcccgaac agcttcaaac gcgagtgggc
     8041 agtctctttt ggtgataaaa aggtctttaa ctacacactg aacggcgaac agtattcatt
     8101 tgatccaatc agcccggatg ctgtagtcac aaccaatatc gcatggcaat acagcgacgt
     8161 taatgtcgtt gcagtgcatc acgccttact gaccagtggt ctgccggtaa gcgaagtgga
     8221 tattgtttgc acacttcctc tgacagagta ttacgacaga aataaccaac ccaatacgga
     8281 aaatattgag cgtaagaaag caaacttccg gaaaaaaatt acattaaatg gcggggatac
     8341 attcacaata aaagatgtaa aagtcatgcc tgaatctata ccggcaggtt atgaagttct
     8401 acaagaactg gatgagttag attctttatt aattatagat ctcgggggca ccacattaga
     8461 tatttctcag gtaatgggga aattatcggg gatcagtaaa atatacggag actcatctct
     8521 tggtgtctct ctggttacat ctgcagtaaa agatgccctt tctcttgcga gaacaaaagg
     8581 aagtagctat cttgctgacg atataatcat tcacagaaaa gataataact atctgaagca
     8641 acgaattaat gatgagaaca aaatatcaat agtcaccgaa gcaatgaatg aagcacttcg
     8701 taaacttgag caacgtgtat taaatacgct caatgaattt tctggttata ctcatgttat
     8761 ggttataggc ggtggcgcag aattaatatg cgatgcagta aaaaaacaca cacagattcg
     8821 tgatgaacgt tttttcaaaa ccaataactc tcaatatgat ttagttaacg gtatgtatct
     8881 cataggtaat taatgatgga caagcgcaga accattgcct tcaaactaaa tccagatgta
     8941 aatcaaacag ataaaattgt ttgtgataca ctggacagta tcccgcaagg ggaacgaagc
     9001 cgccttaacc gggccgcact gacggcaggt ctggccttat acagacaaga tccccggacc
     9061 cctttccttt tatgtgagct gctgacgaaa gaaaccacat tttcagatat cgtgaatata
     9121 ttgagatcgc tatttccaaa agagatggcc gattttaatt cttcaatagt cactcaatcc
     9181 tcttcacaac aagagcaaaa aagtgatgaa gagaccaaaa aaaatgcgat gaagctaata
     9241 aattaattca attattattg agttcccttt atccactatc aggctggata aagggaactc
     9301 aatcaagtta ttttcttacc agtcattaca taatcgttat tatgaaataa tcgtttgcac
     9361 tgtctctgtt attcaggcaa tttcaataaa ggcacttgct cacgctctgt cattttctga
     9421 aactcttcat gctgcatttc gatttttctt ctaaaatcat aacttgcata cctgttcttt
     9481 ttatgtatat ccagatctat ttttgtgacg agttcaaacc acaatgatag cactccccat
     9541 gcagcgttat gaaacatttc cgcaacagat ttagagtctg tattaaccgc ttcagtcatg
     9601 tagtcgctaa tacgttctgc agtgattttc gttatttcat cgtaattcat tttatcccct
     9661 acaagactat ttaatggaat gaaaaatatt tggtctgata acaatgctcc tgaattaaca
     9721 cctgcaggct atgttaaagt tgccctcaca cacaatctac ctgttttctc gtgtgtgtca
     9781 caggcgcaat acataaatag tctggccatt aagttcgtaa cggatttcgt actcgccagc
     9841 aaaaatccgt ctgacatccc tggtttcaaa ctgaaacaac tgttcatccc atacgtggat
     9901 gagttaacag aatgaccggc gcctgtgtca gggactacac cgttttggct gcagctggtt
     9961 tactgaccaa cgccagaaaa tcatataact gcgtcaatgt cagcgtatcc agcgcgactg
    10021 tattattttt ctctgcaacc agaatattgc attaggttat taccaggtgt tctaatgtac
    10081 aggtaaagac gttctttata atctgaaatc tctactttta atccctcaga ctttgcctgc
    10141 tgaaagccct cgtcaaggtg cgcttttatt aggttaatgt ctggatgtct tgtatcgggg
    10201 gcaattctgt attggtgtaa cgatggggtg gatgttccct gcaataagac ctccagatag
    10261 agattggcac aaccattccc atta
//
LOCUS       DAZSBF010000146        10016 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZSBF010000146 DAZSBF010000000
VERSION     DAZSBF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.148"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..120)
                     /locus_tag="ACHXWD_002938"
     CDS             complement(<1..120)
                     /locus_tag="ACHXWD_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR7621798.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQ"
     gene            176..526
                     /locus_tag="ACHXWD_002939"
                     /pseudo
     CDS             176..526
                     /locus_tag="ACHXWD_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(510..1148)
                     /gene="ytfB"
                     /locus_tag="ACHXWD_002940"
     CDS             complement(510..1148)
                     /gene="ytfB"
                     /locus_tag="ACHXWD_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR7621799.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1367..1987
                     /gene="fklB"
                     /locus_tag="ACHXWD_002941"
     CDS             1367..1987
                     /gene="fklB"
                     /locus_tag="ACHXWD_002941"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR7621800.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2296..3708
                     /gene="cycA"
                     /locus_tag="ACHXWD_002942"
                     /gene_synonym="dagA"
     CDS             2296..3708
                     /gene="cycA"
                     /locus_tag="ACHXWD_002942"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR7621801.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3753..4415)
                     /gene="ytfE"
                     /locus_tag="ACHXWD_002943"
     CDS             complement(3753..4415)
                     /gene="ytfE"
                     /locus_tag="ACHXWD_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR7621802.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4523..5488)
                     /locus_tag="ACHXWD_002944"
     CDS             complement(4523..5488)
                     /locus_tag="ACHXWD_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7621803.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5596..6456)
                     /gene="qorB"
                     /locus_tag="ACHXWD_002945"
     CDS             complement(5596..6456)
                     /gene="qorB"
                     /locus_tag="ACHXWD_002945"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR7621804.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6545..6925
                     /locus_tag="ACHXWD_002946"
     CDS             6545..6925
                     /locus_tag="ACHXWD_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR7621805.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(7043..8986)
                     /gene="cpdB"
                     /locus_tag="ACHXWD_002947"
     CDS             complement(7043..8986)
                     /gene="cpdB"
                     /locus_tag="ACHXWD_002947"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR7621806.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            9176..9916
                     /gene="cysQ"
                     /locus_tag="ACHXWD_002948"
     CDS             9176..9916
                     /gene="cysQ"
                     /locus_tag="ACHXWD_002948"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR7621807.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2333 a         2533 c         2600 g         2550 t
ORIGIN      
        1 ttgccatgtt ttacggcagt gagagcagag atagcgctga tgtccggcag tgcttttgcc
       61 gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaaa cagaagccac
      121 tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca tcaccgctaa
      181 cccttggttt ttaaaaattg tgcatagtga aaatcaaagt aaaggtgtgc attatgcaaa
      241 atcactgcgt ctcttagaaa ttaaccatgc gcacttgcag ttaatggaat ccttattgga
      301 tgaagggaag aaacacaata tttttaagcc agatatcgac ccattgcagg tgaatattaa
      361 tattgctgcg cttgggggat attatttgat caaccagcat acgcttggcc tggtttatca
      421 catcagtatg gtttcacccc aggcactgga agccagacgt aaggtcatca aagagacaat
      481 ccttagctgg cttttggttg acccttcatc taccgcgcac gaataaaact gccatccggc
      541 tggcgggtaa acaggacctg ttgattattc cccgtatcaa tggttaagcc cgtcaccacg
      601 ccgctggcgt tctggcggat tttcaccatc tgcccatttt gcaaattact cagcggctta
      661 cccgcgcctt ctacctgcgc cattgcatag acatcggtag cgggcagtcc gtggtcgcgg
      721 aacagctgcg ccatcgtttt accgggttcg acacggtaag agcgccattg attatcaatt
      781 ccgctatctg gctggaatgg ttgcgtttgg gtagtttgcg gctgttcttc tggctggcct
      841 tcctgtatag gttctggagc gaccggagcc acctgatcag gatcattttg tggtgtgact
      901 aattgcgccc gcagctgttc ttctgtcggc ggttgtgatt gcgactgaat atctaactgc
      961 gcttcacgcg tcaccaccgg cgcgttgggc gtatcatcag atggaagcag aaatccgacc
     1021 accaatacaa tggcggcaat aatgatcccc cgacgatgca tcggcggcag cgggtccatg
     1081 aagcgaaaat tgtccggcgc gtgccagact ttctccaggg ttggttttag ttcaaagcgc
     1141 ccgggcatgg tttcctcctg ctccgcgtct tgttcctcaa tcatagccta tgaataagct
     1201 aacgctatga tgtccgtggt aaacccgcct ttattatatt catacgggat attgctattg
     1261 tttctttttc cccgggattt gtcatcattc ctgcgacaaa gttttaccca aagaagtgtg
     1321 gctgatatgc tgcccgctac ttttataccc taagaaagga aatacgatga ccaccccaac
     1381 ttttgacacc atcgaagcgc aagcaagcta cggcattggt ttgcaggtag ggcaacaact
     1441 gagtgaatct ggcctggaag ggctgctgcc agaagcactg gttgcaggta ttgccgatgc
     1501 gctggaaggc aaacatccgg ctgtgccggt tgatgtggtg catcgcgcgc tgcgtgaaat
     1561 ccacgaacgc gccgatgccg ttcgtcgtca gcgtttccag gcgatggctg ctgaaggtgt
     1621 gaaatacctg gaagaaaacg ccaaaaaaga aggtgtgaat agcaccgaat ctggcctgca
     1681 attccgcgtg atcaaccagg gtgaaggcgc aattccggca cgtaccgacc gcgttcgtgt
     1741 tcattacacc ggtaaactga tcgacggcac cgtgtttgac agctccgttg ctcgtggtga
     1801 acccgctgaa ttcccggtta atggcgtgat ccctggctgg attgaagcac tgactctgat
     1861 gccggtaggt tctaaatggg aactgactat cccgcaggaa ctggcatatg gcgagcgcgg
     1921 cgcaggcgca tccatccctc cgttcagcac cctggtgttt gaagtcgaac tgctggaaat
     1981 cctctaagca gcgcattctg ttcccctcga accagagggg agcaggcatt cagcaataaa
     2041 cccttcagtt tgccaaacgg cgctattttg tgttgcaaag accccgtaag cgtgtatttt
     2101 tgtgagctgt ttcgcgttat caccgtgata tgacactcac tttaaacata aaattaacat
     2161 tagatctaaa tcttagtatt catcccgcgt attgttacct aatatcgatg agtcccgata
     2221 cagattcgtc gtatcataga ctgactaaag gccgtagagc ctgaacaaca cagacaggta
     2281 caggaagaaa aaaacatggt agatcaggta aaagtcgttg ccgatgatca ggctccggct
     2341 gaacagtcgc tacggcgcaa tctcacaaac cgacatattc agcttattgc cattggcggt
     2401 gccattggta cggggttgtt tatggggtcc ggcaaaacga ttagccttgc cgggccgtcg
     2461 atcattttcg tttatatgat catcggtttt atgctctttt tcgtgatgcg ggcaatgggg
     2521 gaattgctgc tttcgaatct ggaatacaaa tcttttagtg acttcgcttc cgatttactc
     2581 gggccgtggg caggatattt caccggctgg acttactggt tctgctgggt tgtaaccggt
     2641 atggcagacg tggtggcgat cactgcttat gctcagttct ggttccccga tctctccgac
     2701 tgggtcgcct cgctggcggt gatagtgctg ctgctgacgc tcaatctcgc caccgtgaaa
     2761 atgttcggtg agatggagtt ctggtttgcg atgatcaaaa tcgtcgccat tgtgtcgctg
     2821 attgtcgtcg gcctggttat ggtggcgatg cactttcagt caccgactgg tgtggaagcg
     2881 tcattcgcgc atttgtggaa tgatggcggc tggttcccga aaggtttaag tggcttcttt
     2941 gccggattcc agatagcggt tttcgctttc gtagggattg agctggtagg tacaaccgct
     3001 gcggaaacca aagatccgga gaaatcactg ccacgcgcga ttaactccat tccgatccgt
     3061 atcattatgt tctacgtctt cgcgctgatt gtgattatgt ccgtgacgcc gtggagttcg
     3121 gtagtcccgg agaagagccc gtttgttgaa ctgtttgtgt tggtaggttt gcctgcggct
     3181 gccagcgtga tcaactttgt ggtgctgacc tctgcggcgt cttccgctaa cagcggcgtc
     3241 ttctctacca gccgtatgct gtttggtctg gcgcaggaag gtgtggcacc gaaagcgttc
     3301 gctaaacttt ctaagcgcgc agtacccgcg aaagggttga ccttctcgtg tatctgtctg
     3361 ctcggcggcg tggtgatgtt gtatgtgaat cctagcgtga ttggcgcgtt cacgatgatt
     3421 acaaccgttt ccgcgattct gtttatgttc gtctggacaa ttatcctttg ctcgtacctg
     3481 gtttaccgca aacagcgtcc tcatctgcat gagaagtcga tctacaagat gccgctcggc
     3541 aagctgatgt gctgggtatg tatggcgttc tttgtgttcg tggtcgtgtt gctgacactg
     3601 gaagatgaca ctcgccaggc gctgttggtc accccgctgt ggtttatcgc actggggctg
     3661 ggctggttgt ttattggtaa gaagcgggct gctgaactgc ggaaataacc gcattatcat
     3721 gctggatggc gcaatgccat ccagctttta gattactcac ccgccagcgc gcgtgggaac
     3781 agtacattgt tttccaggct gatgtgatcc atcaggtcat caatcagttc attaataccg
     3841 ttatacatcg ctttccaggt ggtgcaggct tccggcggcg gtgtgacgtt attggtggtg
     3901 tgtttaatca cttccagcag ttcgcccgct tcatcgtgct cgctttccat cacgctgatt
     3961 ggtcccattg cctggctgcc catgccttgt ttgatcatcg ggaagaggat ctgctcttct
     4021 ttcatcatgt ggctggatag ctcttcatgc agcatagtca ggtattttgt cagccctttt
     4081 ggcacgctcg gtttgtcggc gtgaacgcgc tcgactttag tcgcttgcag gatcagctcc
     4141 ggcagttgct cgcggtgacg atcgtggtag cgcacgatga tatggtcaat gatttctgcc
     4201 agcggggcgc tacgccagtc tttctcaatc ggttgttcag caagctttgc cagttcagct
     4261 tcaatgacct caacatccag ttctttacgt gccgccgcgc gcgccagcgt ttgcttaccg
     4321 ccacagcagt aatccatatc atatttacga aacagagctg aagcgcgagg aatagagagc
     4381 gccagttcac ctaaaggttg gtcgcgataa gccatagctg atacctcatt cttaataata
     4441 taagatgtat tttaaatgca tctttaaggc aaaagctata acccttacgt agcagaggct
     4501 tatttaactc actgccagcg tgtcaacttt ctgagacaga ttcagttaaa ggtttttctg
     4561 gttttgctct gaccgcaatg accacgccaa tcactaacag cgcgataccg ctcagcgtca
     4621 ttagcggcgg catttgctgg cggagtaaaa aggtgtacag caaacctgcc agcgtttcga
     4681 aaacaatcag cggcccgaga atcactgtcg gtaatcgctg gctggcgacg ttccagcaga
     4741 gtgcgccaac ccatgagcaa agcacggcta tcgcaaccat cagactaata aacaccagcg
     4801 gacgggggcc aaaaggtaag gagaagtccg gcgtttgtat attcagccag taacaggcga
     4861 cgagatagcc gatgagagaa accggcagtg tgaccagcgc ctgcgccgtt gcccacatca
     4921 tcggatgttt gtcgggattt tcccgcagcc agcgggcgtt gcgcagggca taccacgccc
     4981 agcagaccac ggaaactaac gccagcacga tgccagaggt ataacgtgcc cagtcaaaat
     5041 cggggagtcc gtggtttaac tcagcaatat tcacacacgc caggccgatg ccaatacaaa
     5101 ccagtgccgg ggcgagtttt ccccatgcga gtttgccgtc gcgctggcta taaagcagat
     5161 tggcaaaaac gggaatcacc accggcaggg tgccgataat catcgtggaa acaggcgcgc
     5221 cagtacgttg aatggcactg gcaaggcaga agtaatagat aaggttgccc atcatggtga
     5281 gcatcaaggc ggtaagccag tcgcgacgcg ccaactgacg cagacgcacg cgtcccagcc
     5341 aggcaatggg cagcgcaatt aaccctaacg ccagataacg ccccatcgac tgcaacatcg
     5401 ccgggtattc cggtacgatc aacggcccga caaaaataag cccccacatc aaccctgcta
     5461 acagggcgta cagcacgccg ctaatcatta ctggcatcca ttggtctgtc agaagaaagt
     5521 tcaggaagca gacatgattg ctcccggcat cccgtcatta tcagagagga tgcgggagga
     5581 tgccacttta attaactagt tattaacatt aaaaagatgg cttacgcttt cggctaacgt
     5641 tgtcgttggg cggccaatca atttgctaag cgttttgctg ttatcaaaca gaccaccttt
     5701 cgatgcgcca acgtcagaat ccgccagcat atccgccagt ccgtcgggca gtccgacgct
     5761 tttcagtgcc gcggcgaaat cggcttcgct cagattttga taggtaacct gtttgccgct
     5821 ctgtttggtc agctctgccg ctaactgtgt caacgtccag gcactatcgc ccgccagttc
     5881 gtaaaccttg ccttcgtgac cggcttcgct aatcacgcgt gccgcagctg ccgcataatc
     5941 tgcccgcgtt gctgaggcga ttttgccatc gcccgccgca ccgataaata cgccgtgttc
     6001 cagtgctgcc ggggcgctgg cgaggtagtt ttcgctgtac cagccgttgc gcagcagggt
     6061 gtaaacgatg ccagaatcag ccagcatttt ctccgtctca atgtgctcat cggcgaggcc
     6121 gagcggggag gtatctgcat gtagcaggct ggtataagcg ataaatttca cgccagccgc
     6181 ctttgcggca ttaataacat tacgatgctg cggggcacgt tgacccactt cgctggaaga
     6241 gatcagcagt agtttttcca ctccctgaag tgcagatgtc agtgcggctt catcgccgta
     6301 gtcagcctga cgcacggtaa tgccttgtgc tgctagagcc tgggcttttg ccggattacg
     6361 aacgatagcc actatttggc tggcaggaac cgttttcatc aaggattcaa taatatagtg
     6421 accaagttgg ccagtggcac cagtaatagc gatcatggga agtctccatc gttttgtctt
     6481 gtgtttaaat acgctaacac ctaaacttac ttttagtaag tacgtacaaa aaggtaagta
     6541 tgaaatgagt caggttagcc tgtcgcaaca actgaaagag ggtaatctct ttgcggaaca
     6601 gtgcccgtcg cgcgaggtgt tgaaacacgt caccagccgt tggggggtgt tgattctggt
     6661 ggcgctacgc gaaggtactc atcgctttag cgacctgcgg cgcaaaatcg gcggggtgag
     6721 cgaaaagatg cttgcgcagt cgttacaggc gttagaacag gatggttttc ttaaccgtat
     6781 cgcgtatccg gtggtgccac cgcatgttga atacagcctc acgccgctgg gcgagcaggt
     6841 gagcgaaaag gttgctgcac tggcggactg gattgagttg aatttgcccg aggtgttggc
     6901 ggtgcgggat gaacgtgcgg cataactgcc ggatgcgcgg cctacggtgt atgacttgta
     6961 gggcggataa gatgcgccag catcgcatcc gccaataaat gccggatgcg attgatgatt
     7021 tataggccga aaaagaagtg ttttacttac tcaaatccac ctgataaatc gcaaacccga
     7081 tatcatcggt cgcgacttta ttcatcggat actgcccttt ctctttaata aacgctgcgg
     7141 ctttatctga cggggaagtt tcgaaacgga tatccagttt cttatcggca gctatcggtg
     7201 ctaaacgcca gttgttatct gccgccgggt gaatttcccc cgcacgtttc gactcatcag
     7261 caatccacgc tgccagcacc gagcggttct catccggtga agcaaaagcg atatggctgt
     7321 cgcccgtacc ggcaaatttg ccgccgtaag cgcgatagtt attggtggca acgaggaaca
     7381 tggcgttcgg atcaatcggc ttgccattaa aggtcaggtt cttaatcctt tccgcattgg
     7441 tattaatcat ctggcactcg ccgtcataac gggcgggctg ggtaacatca atctgataat
     7501 tcacaccatc aataacatca aagttataag tgcggaaacc atcccagttg atgagtgact
     7561 gtggtttcgt gctgttagga tcaatctggt taaactgtcc ggcggagcac tccagccact
     7621 ctttcacctc tttaccgctg gctttcacta caatcagcgt attggggtag agataaagat
     7681 cggcggcatt acggaaggtc aactggcctt tttccacctc cacatagctt gccgggtcat
     7741 ttttgcgacc accaacttta aacggtgcgg cagctgaaag caccggcagt tttgccagat
     7801 ccggatcgcc ctgaatgtaa tgttcgacat acgctttttg cgcgttgttc acgacttgca
     7861 cggtcggatc gtcctgcacc agcgccagat agctatacat attgtcggca gatttaccga
     7921 ttggcttgct gacgaactgg cgtgtggcat cgtgatcggc tttgagtgtt tctaccagct
     7981 tgctgtcttc cgccgcgagg gattttttat tggcgatgtc gtaaatcggc cgagcttccg
     8041 ctttcgcctg cgtcacctgc catttaccgc tgtcattact gagttgtaag tcgaccaccc
     8101 caagatgatc gccccacatg cccggcatta ccgccggaac accattcagc gtgcctttgg
     8161 tgatatcagc cccttcgata tcagcaaaat ctttacccgg gaaaacggcg tgagcatgac
     8221 caaacataat ggcgttaacg cccggaattt cactgaggta ataaactgag ttttccgcca
     8281 tcactttata cggatcggca gacagcccgg aatgtgccag aacgacaacg acatcggcac
     8341 ctttctcgcg catttcaggc acgtatttgc gcacggtttc ggtaatatca ttcaccgtca
     8401 ctttcccgga taaattagct ttatcccagc ccatgatttg cggcggcacg acgccaatat
     8461 agccaatctt cagcgtctgt ttttttccgt ctttatcgac cacttcggta tctttaatta
     8521 aatacggtgt aaacattggc tgtttggttc tggcgtcaat gacgttggca tttacataag
     8581 ggaatttcgc tcccgccagc gcatttttca ggtaatccag accgtagtta aattcatgat
     8641 tgccgagtgt accgaccgta taatccagcg tattcagcgc cttataaacc ggatgaacat
     8701 cacctgtttt taatcctttc gccgacatgt aatctgccag cggactcccc tgaatcaaat
     8761 cgccgttatc gaccagtacg ctgtttttca cttcattgcg ggcatcgtta atcaggcttg
     8821 ccgtacgtac cagtccgaat ttttccgtgg cggtgtcttt gtaataatcg aaatccatca
     8881 tgttgctatg cagatcagtg gtttccatga tacgcagatc gaccgtcgct gcattcacac
     8941 tggcggcaat cagcgtggcc aggagcgttg cgctaaactt aatcatcagg gacatccttt
     9001 tatcatcggg aatacgaaag aaaagggaga ataaacgtct tacttataga acagtgaaga
     9061 atgccacaat tttacgcttt gaaaatgatg acactatcac agttggcgca ttcattaacg
     9121 atagggtata agtaaaacaa taagttaaca ccgctcacag agacgaggtg gagaaatgtt
     9181 agatcaagta tgccagcttg cacggaatgc aggcgatgcc attatgcagg tctacgacgg
     9241 gacgaaaccg atggacgtcg tcagcaaagc ggacaattct ccggtaacgg cagcggatat
     9301 tgccgcacac accgttatca tggacggttt acgtacgctg acaccggata ttccggtcct
     9361 ttctgaagaa gatcctcccg gttgggaagt ccgtcagcac tggcagcgtt actggctggt
     9421 agacccgctg gatggtacta aagagtttat taaacgtaat ggcgaattca ccgttaacat
     9481 tgcgctcatt gaccatggca aaccgatttt aggtgtggtg tatgcgccgg taatgaacgt
     9541 aatgtacagc gcggcagaag gcaaggcctg gaaagaagag tgcggtgtgc gcaagcagat
     9601 tcaggtccgc gatgcgcgcc cgccgctggt ggttatcagc cgttcccatg cagatgcgga
     9661 gctgaaagag tatctgcaac agcttggcga acatcagacc acgtccatcg gctcttcgct
     9721 gaaattctgc ctggtggcgg aaggacaggc gcagctgtac ccgcgcttcg gaccaacgaa
     9781 tatttgggac accgccgctg gacatgctgt ggctgcagct gccggagcgc acgttcacga
     9841 ctggcagggt aaaccgctgg attacactcc gcgcgagtcg ttcctgaatc cggggttcag
     9901 agtgtctatt tactaaattc agatggcaga aacagtgtat ttcctgattc tgccatcctg
     9961 atttctccca acctaaaaag ttataaataa aaagagattg tatttaaagt gcaaaa
//
LOCUS       DAZSBF010000147         9948 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZSBF010000147 DAZSBF010000000
VERSION     DAZSBF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.149"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..190
                     /gene="bdm"
                     /locus_tag="ACHXWD_002949"
     CDS             <1..190
                     /gene="bdm"
                     /locus_tag="ACHXWD_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR7621808.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            292..429
                     /gene="sra"
                     /locus_tag="ACHXWD_002950"
     CDS             292..429
                     /gene="sra"
                     /locus_tag="ACHXWD_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR7621809.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            586..2283
                     /gene="maeA"
                     /locus_tag="ACHXWD_002951"
     CDS             586..2283
                     /gene="maeA"
                     /locus_tag="ACHXWD_002951"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR7621810.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDIGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2417..3419
                     /gene="adhP"
                     /locus_tag="ACHXWD_002952"
                     /pseudo
     CDS             2417..3419
                     /gene="adhP"
                     /locus_tag="ACHXWD_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3565..3849
                     /locus_tag="ACHXWD_002953"
     CDS             3565..3849
                     /locus_tag="ACHXWD_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR7621811.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHXWD_002954"
     CDS             complement(4077..4730)
                     /gene="fdnI"
                     /locus_tag="ACHXWD_002954"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR7621812.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHXWD_002955"
     CDS             complement(4723..5607)
                     /gene="fdnH"
                     /locus_tag="ACHXWD_002955"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR7621813.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHXWD_002956"
     CDS             complement(5620..8667)
                     /gene="fdnG"
                     /locus_tag="ACHXWD_002956"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8080..8082),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR7621814.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8899..9780
                     /gene="yddG"
                     /locus_tag="ACHXWD_002957"
     CDS             8899..9780
                     /gene="yddG"
                     /locus_tag="ACHXWD_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR7621815.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2410 a         2607 c         2507 g         2424 t
ORIGIN      
        1 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcaagg
       61 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      121 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      181 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      241 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      301 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      361 gttaccgaag gtgacaaatc cagcgtggta aataacccaa ccggcagaaa acgccccgct
      421 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      481 gtgtttttgt ttttatctgc tttatacttg gggccgacgc cctggcggta aagcaaagac
      541 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      601 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      661 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      721 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      781 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa
      841 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
      901 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
      961 tttatctctt accagaaccg ccacaatatg gacgatattc tgcaaaacgt accgaaccat
     1021 aatatcaaag tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc
     1081 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1141 agcccggcgt atacccttcc ggtggtgctg gatatcggaa cgaacaacca acaactgctt
     1201 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1261 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1321 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1381 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1441 agccgagcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1501 gcgggatgcg gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag
     1561 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg
     1621 ccgaacctgc tgcctttcca gaccaaactg gtgcagaaac gcgaaaacct cagtgactgg
     1681 gataccgaca gcgatgtgct gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1741 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcatccg tgagatgcat
     1801 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     1861 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac gggcagcccg
     1921 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     1981 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2041 atgctgatgt cggcaagtga aacgcttgca cagtattcgc cgctggtgct gaacggcgaa
     2101 ggtctggtac taccggaact aaaagatatt cagaaagtct cccgtgcaat tgcgtttgcg
     2161 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cctctgccga agctttgcaa
     2221 caagccattg acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2281 taagcctgcg cccggtagtg aaggctaccg ggcaattttc ctctcccttt ttcagatctc
     2341 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2401 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac
     2461 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2521 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ccgacgtaat
     2581 tctgggccat gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa
     2641 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2701 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2761 cggaagagtg catcgtggtc gccgattacg cggtaaaagt gccagatggt ctggactcgg
     2821 cggcggccag cagcattacc tgtgcggggg tcaccaccta caaagccgtt aagctgtcaa
     2881 aaattcgtcc cgggcagtgg attgctatct acggtcttgg cggtctgggt aacctcgccc
     2941 tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc cattgatgtc aatgatgagc
     3001 agttaaaact ggcaaccgaa atgggtgcag atttagcgat taactcacgc accgaagacg
     3061 ccgccaaaat tgtgcaggag aaaaccggtg gcgctcactc tgcggtggta acagcagtag
     3121 ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg
     3181 tcggtctgcc gccggagtct atgagcctgg atatcccacg tcttgtgctg gatggcattg
     3241 aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac cgaagccttc cagtttgccg
     3301 ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt agcggacatc aacaccatct
     3361 ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt gattgatttc cgtcactaag
     3421 aggtctttgc tgcgactccc atgttcgggt cgcagcatcg cacactctcc aacatgaaat
     3481 ggctaaatgg attattgttg cgtaggaagg aacaataatg atagtgttcc cagagaagga
     3541 acgctactga gagtaaagac actaatgaaa atggccaatc atccccgccc gggggacatt
     3601 attcaggaat cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa
     3661 attgcgccct caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg
     3721 gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac
     3781 gcctggagtc tggcagaggc agaaaaaacg gtggatgtgt cgcgattacg ccgtctggtt
     3841 acgcaataaa aaatggcacc ttataaagcg ccatttgaaa ctgatgacaa acgcaacatt
     3901 gcctgatgcg ctacgcttat caggcctaca cgttcacttc aatctattgt atttgaaggg
     3961 ttttcatgcc ggataaggca ttcacgacgc atccgggatg aacaaagccc acgttgtcta
     4021 caatatgaaa atggcgcctt ttacagcgcc attttcagct taaagttcaa aagagattat
     4081 atcccttctt cactctcttt tttcgcttct gccttctcga tttcacgata ccagcgcgga
     4141 tggtgtttct tcgcccagcg acggctcacc ttcccttcga tcatcccttt aatcgaccct
     4201 ttcacccaga atgccatata catatggatc aggatggcgt ggatcaggat gatacccgca
     4261 gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg ggaagtactg cgcaaagtac
     4321 ggacgccaga taatcacccc ggtcaccagc agcacgaaaa tcatgctcat gatcgaccag
     4381 aacatcatct tttgcccggc gttgtactta ccaacatccg ccactttgtg ctcattgcct
     4441 tttaatactt cgacaatgtt caacagccac ggaatatctt tcttatccgg gatgttgtga
     4501 tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa tcgcaatgcc gaagaacggg
     4561 tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg tcagccattg cagcgtcggg
     4621 aagaagaacg aaatcccgga cagcgccacc aggaagaagc aaatcaccac ggtccagtga
     4681 caggcgcgat caataaattt ggtgcgcaca atcattttcg acttactcat gatgatcctc
     4741 ctcgtcatcg tccacttcct tattcgggcc aataccgatg tagtggaaaa tcaacccggc
     4801 aaaagtggca ataaagccag ccgctgccag cggtttcaac gcgcctttcc acaggcttac
     4861 cgaggtgtcg atcttcggat ctttcggcag accgtaatac agctccggct gatcggcgtg
     4921 atgcagcacg tacataacgt gcgtaccacc gacccctttc gggttgtaga cgccagcatg
     4981 ttcgtaacca cgcgctttca gtttcgccac gcgctcttcc gccagctcca gcatctcctt
     5041 cttggtgccg aagtggatag ccccggtcgg acaggttttc acacaagccg gttcctggcc
     5101 gacgctgacg cgatcgacgc agagcgtgca tttatatacc cggttatcct ctttgttgag
     5161 gcgcggaata ttaaacggac acccggcaat gcagtaacca cagccgatgc agttttccga
     5221 ctggaaatcg acaatcccgt tagcgtactg aatgattgca ccagcagacg ggcacgcctt
     5281 caggcagccg ggatcttcac agtgcataca gccgtcttta cggatcagcc actccagctt
     5341 gccgttctgt tcggtttcgc taaagcgcat caccgtccag gacttggcgc tcagatcggc
     5401 ggggttatcg taaaccccga cgcagtgccc cacttcatca cggatgtcgt tccactccga
     5461 acacgccacc tgacaggctt tacagccgat acaggtggaa acgtcgataa gttttgcgac
     5521 ttctgctttg taatcacgca cctgagaagg cggcgtgatg gagttagttg cggacctttt
     5581 gataatatcc tgcgtttcca tagccatctg ttcgccccct tacgccttct cgatgttgac
     5641 taagaacgct ttatattccg gcgtttgcga gtttgcatca ccgacattcg gcgtcagagt
     5701 gttagcgata taacctttac gcgcgacacc ctcaaagccc cagtggattg gaatacccac
     5761 cgtttcaacc tgctgaccgt ttacattcag cggtttcaga cggcgcgtta ccaccgccac
     5821 cgcgcggata aagccacgct tgcttgagac agtgacacga tcgccattat taatgccttt
     5881 cgccgccgcc agcgtttcgc tgatttccac aaactgttcc ggctgagcaa ttgcgttgag
     5941 caatgcgtgc ttggtccagg tgtggaagtg ctcggtcaga cgataggtcg tacccacata
     6001 cgggaactgc tcttttttac ccatccgcag cgcgtcttgt tcatacagac gaacaaccgg
     6061 gttagacacc acgttcggat gcagcgggtt agtgcccagc ggcgtttcaa tcggctcgta
     6121 gtgttccggg aacggacctt ccgccatttt gttgatggca aacaggcgtc ccatcccttc
     6181 cggctgcatg ataaacggcc cggttggcgt acccggtgcg gcattgccga agtccggaat
     6241 atcgttaccc gtccacttgc tgccgttcca ctggatcagc atccgtttcg gatcccacgg
     6301 tttaccattg atatccgccg aagcacggtt gtagagcacg cgacggttga gcggccacgc
     6361 ccaggcccat cccagcgtat tccccagacc ggatgggtcg gagttatcgc ggttagccat
     6421 ctggttgccc tgctctgtcc agctaccggt gtagatccag caagaagatg cggttgtacc
     6481 gtcatcacgc agatgcgcaa agctactcag caactgacct ttcttcgcaa tcagcacgcc
     6541 attagcgtca tagagatctt ccagcgcata gccgttattc tctttcgcca cttcgtcaga
     6601 ttgcggttcg tgcggctgct tgtagttcca gctcatcttc atcagcggtt ctacgccttt
     6661 accaccttcg gcctggtaca gctcgcgcag atgatggtag atacccgcca gaatttcgcc
     6721 gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag tgccactgca accagcgacc
     6781 ggagttagcg atagaaccat cttcttcggc aaagcaggtc gaaggcagac ggaatacttc
     6841 agtctgaata gacgccggat cgacatcgtt cgactcgccg tggttctgcc agaaggtaga
     6901 ggtttcagtc accagcggat cgataaccac catgtacttc agcttgctca ggcagctcac
     6961 cactttgttt ttgtccggga aggacgcaac cgggttaaag ccctggcaga agtaaccggt
     7021 gactttgcct tcgtccatca tgttgaaata cttgatgacg tcgtaggtct ggtcccactt
     7081 cggcagccag tcatagcccc agttgttctc tttctgcgcg gcatcgccat agaaagattt
     7141 catcaggcta acgaagaact tcggatagtt gctccagtag ttcacctgat cagccagcgt
     7201 cgctttcggc gtgttcgctt ccagatacga ctgcaaatca acctgttttt ctgacggaag
     7261 cgtcagataa cctggcaggc tggtagagag cagacctaag tcagtcagcc cctgaatgtt
     7321 ggagtgacca cgcaatgcgt tcacgccgcc accggccata cccatgttac cgagcagcaa
     7381 ctggatcatc gccatagtac ggatgttctg cgcacccacg gtgtgctgcg tccagcccag
     7441 cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg gaggccagca cttcacacac
     7501 tttcaggaag tcggcttttg gcgtaccgca gatgttttca acgacgtccg gcgtgtagcg
     7561 ggaaacgtgc gctttcagca ggttccacac acagcgcgga tgagtcagtg tttcatcgcg
     7621 ttttgcatag ccgttttcat cgaactgata gttccaggac gacttatcat actggcgttt
     7681 ttcagcgtcg tagccgctga acaggccgtc ttcgaaagca aaatcatcac gcaccagcag
     7741 gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt ttgttgtttt cgatcaggta
     7801 gcgcaaaacg ccagacagga acgtaatgtc cgtaccggaa cgaataggtg cgtaaatatc
     7861 cgccacagaa gcggtacgcg taaaacgagg atcgacaacg atcaaggttg catcgttgtt
     7921 gtttttcgct tccatcgccc agcggaaacc gacgggatgc gcttcagcag cgttaccgcc
     7981 cattaccatt acgacgttag cgtttttgat atccacccag tggttggtca tcgcaccgcg
     8041 accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt cagacgcgcg cctggttgtc
     8101 taccgccagc atcccgaggg agcgggcaaa tttctgggtc agcatcccgg tttcgttgct
     8161 ggcaccggag gcacacagca taccggtaga aagccaacgg tttaccgtta cgccctgctc
     8221 gttcttttca ataaagttag cgtcacggtc agctttcatc agcttcgcaa tacgggagaa
     8281 tgcttcttcc cagctaatgc gctgccattt gtcagaacct ggcgcacgat attccgggta
     8341 gcgcagacgg ttttcgctgt tgacgtaatc cagcaaaccg gccccttttg ggcacagcgc
     8401 accacggctt accggatgat ccgggtcacc ttcaatgtga taaatcgctt ctctggcgtt
     8461 tttcgcgcca tcacccaggc tatacatcaa tagcccgcaa cctacggaac agtatgtgca
     8521 ggtgttacgg atctctttag cgcgtaataa tttgtagttt cgcgcctgag ccagtgcttg
     8581 cttcggggca aagcccaatg ccgctaccgt tgttccagcc ataccgcccg cgcagatttt
     8641 aaaaaattgt ctgcgactga cgtccattgc tttcctcttt tttcaggggt attactgcgc
     8701 gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa atcaaaaaga acacgtgtat
     8761 gccacaaaat aatccgcctg cgcaaattaa cctacctcaa tagcggtaga aaaacgcacc
     8821 actgcctgac aggccagtta aaaaaatgct ataaaattca gcttaatttt taacggcaag
     8881 agagacaaaa cagcgagcat gacacgacaa aaagcaacgc tcatagggct aatagcgatc
     8941 gtcctgtgga gcacgatggt aggattgatt cgcggtgtca gcgaggggct cggtccggtc
     9001 ggcggcgcag ctgctatcta ttcattaagc gggctgctgt taatcttcac ggttggattt
     9061 ccgcgtattc ggcagattcc gaaaggctat ttactcgccg ggagtctgtt attcgtcagc
     9121 tatgaaatct gtctggcgct ttccttaggg tatgcggcga cccgtcatca ggcgattgaa
     9181 gtgggtatgg tgaactatct gtggcccagc ctgacaattc tctttgccat tctgtttaat
     9241 ggtcagaaaa ccaactggct gattgtacca ggattattat tagccctggt cggcgtctgc
     9301 tgggtgttag gcggtgagaa tgggttacat tatgatgaaa tcatcaataa tatcaccacc
     9361 agcccattga gttatttcct ggcgtttatt ggtgcgttta tctgggcagc ctattgcaca
     9421 gtaacgaata aatacgcacg cggatttaac ggaattaccg tttttgtcct gctaacggga
     9481 gcatgtctgt ggatttacta ttttctcacg ccacaaccag aaatggtatt tagcacgccc
     9541 gtcatgatta aactcatctc tgcggcattt accttaggat ttgcttatgc tgcatggaat
     9601 gtcggtatat tgcatggcaa tgtcaccata atggcggtag gctcgtattt tacgcctgta
     9661 ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc tgtcgttctc gttctggcaa
     9721 ggcgcgctaa tggtctgcgg cggttccctg ctctgctggc tggcgacacg tcgtggttaa
     9781 aataattgcc gggtctacgg cccggcagtt aatcagataa attccgacaa tattaaaaaa
     9841 taaatttcaa ataccagtaa ttcaccttat atctcatttc gacttcgatc acatatcgat
     9901 aaataatatt atttcatcta tttttctcat tgtttttaat caccattc
//
LOCUS       DAZSBF010000148         9902 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZSBF010000148 DAZSBF010000000
VERSION     DAZSBF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9902
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.150"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..160)
                     /locus_tag="ACHXWD_002958"
     CDS             complement(<1..160)
                     /locus_tag="ACHXWD_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621816.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFSLNYKL"
     gene            238..1332
                     /gene="ddlA"
                     /locus_tag="ACHXWD_002959"
     CDS             238..1332
                     /gene="ddlA"
                     /locus_tag="ACHXWD_002959"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR7621817.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1356..1568)
                     /locus_tag="ACHXWD_002960"
     CDS             complement(1356..1568)
                     /locus_tag="ACHXWD_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR7621818.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1828..2136
                     /locus_tag="ACHXWD_002961"
     CDS             1828..2136
                     /locus_tag="ACHXWD_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR7621819.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2195..3289)
                     /gene="yaiW"
                     /locus_tag="ACHXWD_002962"
     CDS             complement(2195..3289)
                     /gene="yaiW"
                     /locus_tag="ACHXWD_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR7621820.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3302..4522)
                     /gene="sbmA"
                     /locus_tag="ACHXWD_002963"
     CDS             complement(3302..4522)
                     /gene="sbmA"
                     /locus_tag="ACHXWD_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR7621821.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4874..6031
                     /gene="ampH"
                     /locus_tag="ACHXWD_002964"
     CDS             4874..6031
                     /gene="ampH"
                     /locus_tag="ACHXWD_002964"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR7621822.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6032..6655)
                     /gene="iprA"
                     /locus_tag="ACHXWD_002965"
     CDS             complement(6032..6655)
                     /gene="iprA"
                     /locus_tag="ACHXWD_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR7621823.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6743..9625)
                     /locus_tag="ACHXWD_002966"
     CDS             complement(6743..9625)
                     /locus_tag="ACHXWD_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR7621824.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITNGGVLDVNTNMNDISSVFAT
                     DTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMNSD
                     GHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYIDD
                     TKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQ
                     DTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGNNA
                     KWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSA
                     TMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMK
                     VDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTE
                     GSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2571 a         2433 c         2365 g         2533 t
ORIGIN      
        1 ttaacttata attaagagaa aaaaactcac cgcattgcca tttaagccag gaataaatta
       61 gcttcccgtt catcattttt gtgatctcgc gcacatcttt gagataacgc actttcttca
      121 ccagtacgtt gcacgctttg tcagcagaag attgcaacaa tctttcagaa ggccgcgaat
      181 cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt ttttaagatg
      241 gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag cggaacatga agtgtctctg
      301 caatcggcaa aaaacattgt cgatgccatt gataaaagtc gcttcgacgt tgtgctgctg
      361 ggcattgata aacaagggca atggcacgtc agcgatgcca gcaattatct gctaaatgca
      421 gacgatcctg cccatattgc gttgcgccct tcggcgacca gccttgcgca ggtgccaggt
      481 aaacatgagc atcagcttat cgacgcgcaa aacggtcagc cgttgccgac ggtggatgtc
      541 attttcccga ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg
      601 gtcgccaatt taccgtttgt aggttctgat gttctggctt cagcagcctg tatggataaa
      661 gatgtcacca aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt tattaccctg
      721 acgcgcgcta atcgtcacaa catcagtttt gccgaagtgg agtctaaact ggggttacca
      781 ctgtttgtaa aaccggctaa tcagggctct tctgttggtg tcagcaaagt aaccagtgaa
      841 gaacagtacg caattgccgt cgatctggcg ttcgagttcg atcataaagt gatcgttgag
      901 caagggatca aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa tccgcaagcc
      961 agcacctgtg gcgagatcgt actcaccagc gatttctatg cctacgacac caagtacatt
     1021 gacgaagatg gcgcgaaagt ggtagttccg gcagccattg caccagagat caacgataag
     1081 atccgggcga ttgccgttca ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta
     1141 gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg agatcaacac actgcctggc
     1201 ttcaccaaca tcagtatgta tccgaagctg tggcaagcca gcggtctggg ttacaccgat
     1261 ctgatcacac gtttgattga gctggcgctg aagcgtcacg ctgcggataa cgcattgaaa
     1321 gccacaatgt aatattttaa cgcccgataa tattcttatt gggcgttttc agtttcttca
     1381 tcgcgacgac gaataatcaa ccccgccagc cagaaactaa tcacccacgt caccagaccg
     1441 acggcatagg tctgccaacc ttttgcttca aatcccagta accccaccac gccattaaga
     1501 atgaatataa ggccaatggc gaacgcatag tagtgccagt cacggcggat ttttacaggc
     1561 agattcatgg cagctccgtt aacgaaaaac aacatccttg catattctgt aacaaccgtc
     1621 tgtggtgtag acaacatttt cgtaattgtt aataaattta acttaaggtt gccattttgt
     1681 aactcacaac gaagattcac gcttcgggaa tattcccaac atcaatttac catgattctg
     1741 atattgacaa tctaaatgac ctgtcagact tgccataagc cgctggtatc acaccagaag
     1801 agaagcagat gacttccgga ggtttttatg gctgatttca ccctgtcaaa atcgctgttt
     1861 agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc gttgtttgtg
     1921 ctgttttgct tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc gcccggcgtc
     1981 tatgagcgtt taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt
     2041 gtcggcacca ttttcgggct gatcccgttt ttagtaggct gcctcatttt tgcagtggtg
     2101 gcgctatggc tgcactggcg acatcgccgc cagtaagatt tacgttgcca gatacggcct
     2161 aaaacgcctt atctggcatg acattcctgt ttgatcactg tttcatacaa cgcgcccgcc
     2221 gttcgtctac gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt ttcgggcttt
     2281 ccagttgaat gccaggcaac atttcgcggg gtagtgattt acccgttttc gcttcggcaa
     2341 gttgataaac tttcttgtac agcgccgtct cctcaaaaga gaagctgtca cctttctcta
     2401 actgacggcg aatttcgctg tcgctcatcc ccagttttcc cgccagtttg cgcgttgcca
     2461 gttcggtttt cccgggttct ttactgtcgt aacgaattaa atcaccgtcc agtgccagct
     2521 tcacgccgct ggccttactg actgcgttct gaaacgcggc attacggctg gcgtaccagc
     2581 cagcgttaaa atcagcaaag cggtatatcg gtgcgctata gctggcggga taattcagta
     2641 aatggtaagt accaaaccac aacccgccgc ggcgactgaa gacttcctga cgaactgtac
     2701 cgtccatttt ccacggatac cctttggtat gctgttcggc aaaagcaatg ctgacctgca
     2761 tcggcccacc ggtgcgcacc ggattaagcg aaccaaacag cgtctgcccc attggtacca
     2821 tgctgatcag atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc gaatctaacc
     2881 gttcgctata acttttgccg tttggcgatt tgatttttag cgcggtatga accagaaaag
     2941 caggaatgtg catccgttca gcacgacggt caatctcttg ccaggcgatc ttgcttaagc
     3001 ccggaacggc cggatctgcc tgataatttg actcctgctg cgccaccgcc agcaccgagc
     3061 agacgttttc cagcgtcggt gccagcccct gactttcaaa ggtgatggcg agatctttcg
     3121 cccaggcatc gcggtctttc acgctcgcag gcatcttctg ccgcacgacg ctcgctacat
     3181 ctatcgcttt ttcgcccttt ttcaacggtt gcggagcctg actactacac cccgctaaaa
     3241 ccagaaccgc caacaatgac agcgaagata gatgattgac gcgcgacatt acttctcctt
     3301 tttagctcaa ggtatgggtt acttcctgaa ttttgtcacc atccagctca tgttcaaagc
     3361 tgcgcagacg tttgtagata gacatcaact caaccagtgt ggtccatgag ttaatcaggt
     3421 actggaaagc accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc aggccgagcg
     3481 taatcgtacc ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg ttatcgacct
     3541 gcaaatagag aatgcgggcg atgttgaaat acatatagtg aaaatagagg cgaaaatagt
     3601 ttttccgtac ggcgctaaac agctcgcgta ccgtaggcgg cgtcgcgcgc gtggcatcgt
     3661 cttcaccata aaccagctct ttacggtagg cggcctctac tcgctggttt ttaaactcca
     3721 gccccggcag tttgatccct accactgcca gccatccggt ccccatcagc gaccagacga
     3781 tagcggcaat caccagacca tacggaatgt gcccgataat cggcagctcc ggcacatgcg
     3841 cggagagcgt taccagcacc ggcaggaagg cgatcaacgt catgatggcg ttgataaaac
     3901 tgacgcccat attctccagc gttgaagcaa aacgcatggt gtcttcctgc acacgctgtg
     3961 cggcaccttc gatatgacgc agttgttgcc agttcgccat gtaatattcg ttcatcgccg
     4021 tacgccagcg gaacacatag tgactgacaa agaagttgtt cagtacactg atcaccaccg
     4081 cgatcagcgc aatccccaga aagacgccca cttcgcggta aaattgttcg atagtgactt
     4141 tatgcggcga acttagcgcg gtttgaatca gatcatagaa cggtgcatac caggcgttga
     4201 cggcgacccc gacttccacc aaaaaccagg tgacgaagat gatcagtgca gtaccgagta
     4261 ttgaccagta ttgccaacgg tgcgggctgt agataaacca gaaaaatgca aaaagtccta
     4321 cgcaaacaat gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa cgcgcggcgc
     4381 taatcgggat ctgcccggaa gcgccggtga tacgcgccac ccagtctccc ccaccggctt
     4441 gccagaagat aacggcaatc aatgcccaaa caaaggccga gagaaaaaac gctcccggct
     4501 ttgggaaaaa agacttaaac atatgcactc ctgctaactt cttatcgttt taacaactgc
     4561 tgtgtattaa ccgcatgacc actacaaagc gtcagccaaa tgaaaccgcc cattgtgacc
     4621 catagcaagg cgcttagttc gccagcctga ccataagaat tgtttcgacg gatttcacaa
     4681 accaaagatt gtttacataa gcggattaac acgcgcagga tcacaaatgc aggcattggt
     4741 gagcaggaaa aagatcgtgt ttacgcgccg ttacgttttt aacactatta ttgcactaat
     4801 tcgccctttg caatccatca atgagtagta taaatacgct cagttacctt cattcaatct
     4861 atggacacca ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg cggcgtcatt
     4921 gacctccgtc catgcggcgc agccgatcac tgaaccggag tttgcctctg atattgtcga
     4981 tcgttatgcc gatcatattt tttacggcag cggtgcaacg gggatggcgc tggttgttat
     5041 cgacggtaac cagcgcgtct ttcgtagtta tggcgaaacg cgacctggta ataacgttcg
     5101 cccgcagctg gattcggtcg tgcgtattgc ttccctcacc aaactaatga ccagtgaaat
     5161 gctggtgaaa ttgctcgacc aggggaccgt aaagttaaac gatccgctaa gtaaatatgc
     5221 tccgccaggc gcacgcgtgc cgacctacaa tgggacgccc attaccctag tgaatctggc
     5281 aacccatacc agcgccctgc cccgtgaaca gcccggtggc gcggcacatc gtccggtgtt
     5341 tgtctggcca acgcgcgagc aacgctggaa atacctttct acggcgaagc tgaaagccgc
     5401 gccaggcagt caggcggcgt actctaacct tgcgttcgac ctgctggcag atgcactggc
     5461 gaatgcgtcc ggcaagcctt atacccagtt gtttgaagag cagattaccc gtccattggg
     5521 gatgaaagac accacctaca ccccctcacc ggatcagtgc cgccgtttaa tggttgctga
     5581 gcgcggtgcc agtccgtgca ataacacgct ggcggcaatt ggcagcggcg gcgtctattc
     5641 cacgcctggc gatatgatgc gctggatgca gcagtatttg tcatctgatt tttatcagcg
     5701 cagtaaccag gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt ttacgaaagt
     5761 gattggcatg gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg tatatatggc
     5821 cccgaaagag ggtcgtccgg ggattattca gaagacaggc ggtggtggcg gtttcattac
     5881 ctatatggcg atgatcccgc agaagaatat tggcgcgttt gtggtggtca ctcgttcgcc
     5941 gttgacgcgc tttaaaaata tgagcgatgg catcaatgat ctggtgaccg agctaagcgg
     6001 gaataaaccg ttggttatcc ccgcatcctg attaatactc tgaaggcaaa cggttgatag
     6061 cgaccagttt gcctttattc atttcgatat agccgccttt acgcaaagca gcgagaactt
     6121 ctgcgacgac agaacgcgat atgcgtgtac gttgatggat atagttcatc acaccaatac
     6181 gtgatcgcaa ttcttcattc cagtcaatca ttgataataa tgttgcgcgg atttgttcgt
     6241 aggaattatg cccaatgagc tgcacgtcgc gtaattccag aattctattt tgccaagcta
     6301 accagtaaaa agcgtctcgc cagagttgat tttgttcaat aagcgtaatg gtttgtttgg
     6361 ctggtagatg atatcccgta caatttcctt ctgatattaa tttgtatggt atatcgtttt
     6421 tcattaaacc atcagccagc cccataatat aaggggcctg ggtaataccg ataagtacgt
     6481 tttcttctct acgcagagag ataactccct ctaaaataac gaaagtatct tcgttattga
     6541 catcactgga aaatataact tgcttttcat tattaaactc gaagcgcgta ccgtatctgg
     6601 acaaacattt atcgagctta ccaaattcct gaagaggttt aactacagat aacatttgcg
     6661 cgtcctttgc agtaatgccc gtcaaatcct tgacgggcat tatttagatt aaattaccag
     6721 tatttcttcg gagtgaagaa tattaccagg tatatttaac acccacgttc gcggaccagt
     6781 cttgatctac gtcaccacca ccgaggtagt tagcatcggt ataggcgctg aagttcttgg
     6841 tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga cccttcagta ccgttatcga
     6901 tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc gtagacgtaa gccagtttga
     6961 agtacggagt cagagcctga tcttcgctgt aggtgaaggt ataacctgca tctacaccca
     7021 gttcataacg catgctgtcg taagactgac cgtcaacttt catgtcgttg ctcagctggt
     7081 agtcatcacc agactggaac agaccagaaa cgctgccgta aggagtcacg taaccagcat
     7141 cacccagttt gaagtcgtaa ccggctttca agccgaagcc ccaagcgtcg gagttggtgc
     7201 taccgtcaac gtaagtaccg ttgctcatgg ttgcagacag gtcgttgttg aagtgagagt
     7261 agctcaagct accatcaaca aagacgttgt tcgcgaagtg agcagaagag tagatgtagg
     7321 cagtctggct gtcttgatct acctgaccag aacggtcatt catgtcacct ttagcgaagc
     7381 ctgcagccgc accgacgatc cacttagcgt tgttaccgtc aactttggta tcaacaccga
     7441 ccatgatgcc gttaacatcc tgatcatagt tgatggtgcc gttgtcgccg ttgaagttac
     7501 caccgaagta gcttacccat gcgccgccgt tatcagccag gccatgacga gagttggtca
     7561 gacgagtacc aacggtgtct tgttccaggt tccagatatt ggtgttcgca gacgggatgc
     7621 tcagcgccat gttagcgtag tcggtcagct ccatctgttg cagaacaacg gtgttaccgc
     7681 gctgttcagc ctgataggtg tatgcaccca ggtcagcttt gttagcagca gagaaggtcg
     7741 cgttggtttt ggtatcatcg atatagatga gctgcttacc tgcgtaatca gcaatcgcac
     7801 cagcgccagt ggagttgtcg atacgaactt tgtagttacc ggttgcagct acaacgttgt
     7861 ctgcagagct gttgtcaact tcgtcgccat tgttaatgtc gttgttacca ttaacggtca
     7921 agtgaccatc agagttcatc gcaataacac cataaccgta gttagctttg ctggtgtcgt
     7981 tggtacgatc gttgacgagg tctgcattca ggatgtaatc attactatga atgtcaaaca
     8041 cacctgcgta aacattaccg ttgttatctt taacgttgcc actggtcagt tccagagtat
     8101 cagttgcaaa tacgctggag atgtcattca tgttggtgtt tacatccagc acaccaccgt
     8161 tagtaatggt attggcatac agcggtgcat atgcccaagc tgaagcagta tcttcaccga
     8221 agttaacggt tgagaaggta tcaatggtca ggtgatcggt gttaacttca ccgttaactt
     8281 ccattgaaga accaccgttc agagtgatgg tgtcagaagt cagagaagaa tctgcactca
     8341 cgttaacctg agaaccgctg ttgatcgcca gggtatcgat cagggaagtt ttcgtggttt
     8401 cccaatcaga accgttgttc agaacaacat cgaatacgcc gctctggaat gcgttagcgt
     8461 tatcaacacg attccagcca tcttcagagt aagaagcgta cgggttcagg ctgtttggtg
     8521 caacgtcgta gaaggcttcg tcagcaacaa caacgtcata atcaatattc gctgaaccaa
     8581 cccatttggt gttgttcaga gtcaggttca ggtgatcgac gttctgatcg tcagttgccc
     8641 aaccgccatg agtgtaagca gtggtagagt tgtcaatcag gtgacccgtc gcgtcaaagt
     8701 tagcgttcca ggtactgtca aatacaacgt caccaagcaa ctgagagttg ttgaacgtaa
     8761 cgttgttgac catacgataa tcagaagtgt catcatcgat gaaggctaac gcaacatcat
     8821 cagcaggaga tgcgccattg ccgtagtcac ttggttcaac agaattgccg aaatgaccac
     8881 gatctgattg ttcgtcttca gtccatgaac cagaagttac agtagagtca ttaacaacga
     8941 tgttgttgtc ccactgatga ccttcggtat tgtaggtcgc attaccgagg tcgtgttgaa
     9001 ccaaagtaat acctgcaaca tgggagttgt tgctgatgtt gatgttactt tcaacatcca
     9061 gagtcacagc ggtaccaaga ccagcggtta ccaaacgatc ataatctgtc ttagatgttt
     9121 taccgtctgc atccagataa gtatcggtga agtagaaggc gtcaaaatca tcgtcaatag
     9181 tagaattagc gatgttcaga gtgaagatgt cgccatcaac ccagtcgtgg ccagtataat
     9241 cgctaccgtt ggtccagacg tagtcgccat aaccaacctg gtaagatgta accataccat
     9301 gaacaattga gttggagatg ttcagtgtat taccggtatc tgtgccataa tcgtggttca
     9361 gataataagt agaaattaca ccatttacag tgtcgccgct gaaagtggta tcaaagtaac
     9421 catcagctgc gtggtcatat acataaccac cgtaagtcag ataatcgtga tcaattttag
     9481 tgctgtcacc ccaattgtag atggtggctg cgtcatcgtg atacccgaag gtgttataag
     9541 tagctgcatt agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta
     9601 caagtttctt tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc
     9661 catcaattat caatgcatag aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta
     9721 cagacgaatg gttcaattat tctttcccct aagtccgaat gtggacatat ataatttatg
     9781 gagaaaaaca cgcatcaaat tgataaaagg acgatatttc aacatgttgc agataatgtt
     9841 atttttgata catacgcgtt ataaatatca aaaacacaaa ccagataaat atcttatcaa
     9901 ta
//
LOCUS       DAZSBF010000149         9896 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZSBF010000149 DAZSBF010000000
VERSION     DAZSBF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.151"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHXWD_002967"
     CDS             complement(113..1108)
                     /gene="gapA"
                     /locus_tag="ACHXWD_002967"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR7621825.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1450..1863
                     /gene="msrB"
                     /locus_tag="ACHXWD_002968"
     CDS             1450..1863
                     /gene="msrB"
                     /locus_tag="ACHXWD_002968"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR7621826.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1905..2177
                     /locus_tag="ACHXWD_002969"
     CDS             1905..2177
                     /locus_tag="ACHXWD_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR7621827.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHXWD_002970"
     CDS             complement(2338..2979)
                     /gene="pncA"
                     /locus_tag="ACHXWD_002970"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR7621828.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHXWD_002971"
     CDS             complement(2990..4006)
                     /gene="ansA"
                     /locus_tag="ACHXWD_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR7621829.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHXWD_002972"
     CDS             complement(4173..6029)
                     /gene="sppA"
                     /locus_tag="ACHXWD_002972"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR7621830.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6190..6741
                     /locus_tag="ACHXWD_002973"
     CDS             6190..6741
                     /locus_tag="ACHXWD_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR7621831.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6858..7901
                     /gene="selD"
                     /locus_tag="ACHXWD_002974"
     CDS             6858..7901
                     /gene="selD"
                     /locus_tag="ACHXWD_002974"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR7621832.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7906..9867
                     /gene="topB"
                     /locus_tag="ACHXWD_002975"
     CDS             7906..9867
                     /gene="topB"
                     /locus_tag="ACHXWD_002975"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR7621833.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2454 a         2472 c         2633 g         2337 t
ORIGIN      
        1 aacaagtgag cgtttccgga ttcttacaca gccactggat cggtcaactg atccttaact
       61 gatcggcatt acgaccgaag tcgctctttt tagatcacag tttcatctca acttatttgg
      121 agatgtgagc gatcaggtcc agaactttgt tggagtaacc ggtttcgttg tcgtaccagg
      181 ataccagttt cacgaagttg tcgttcagag cgataccagc tttagcatcg aacacggaag
      241 tgcaaacttc gccgttgaaa tcggtagata ctacgtcatc ttcggtgtag cccagaacgc
      301 ctttcatttc gccttcagca gcggctttaa cggcagcttt gatctgctcg taagttgcag
      361 ctttttccag acgaacggtc aggtcaacta cggatacgtt cggggtcgga acgcggaacg
      421 ccataccagt cagtttgcca ttcagttctg gcagtacttt acctacagct ttagcagcac
      481 cggtagagga cgggatgatg ttctgggaag cgccgcggcc gccgcgccag tctttgtgag
      541 acgggccatc aacggttttc tgagtagcgg tagtagcgtg aacggtggtc atcagacctt
      601 cgatgatgcc gaagttatcg ttgataactt tagccagcgg agccaggcag ttggtggtgc
      661 aggaagcgtt ggaaacgatg tcctggccag catatttgtc gaagttagcg cctttaacga
      721 acatcggagt gttgtcttta gacggaccag tcataaccac tttcttcgca ccagcggtga
      781 tgtgtttacg agcagtttcg tcagtcagga acagaccagt tgcttcagcg acaacgtcaa
      841 caccaacttc gtcccatttc aggttagccg gatcacgttc agcggtaaca cggatttttt
      901 taccgttaac gatcagatga ccgtctttca cttcaacggt accgtcgaaa cggccgtgag
      961 tggagtcata tttcagcatg tatgccatgt aatcagcgtc taacaggtcg ttgattgcaa
     1021 cgatctcgat gtcagaacgt ttctgagcag cacggaaaac aatgcgaccg atacggccaa
     1081 aaccgttgat acctactttg atagtcatat attccaccag ctatttgtta gtgaataaaa
     1141 ggttgcctgt aaaattacaa aaaccttacg cagcgtcaag cggaatcgtg tcaatcattg
     1201 cgacaaatca atcctgtgcc taagcattac gcgactgact cgcctcactc ttcctttggg
     1261 cttgaaacca catggggtcg gcgccccgaa ttttaaaggg caattacgat aaaaatgtga
     1321 ttttcatcac gatttaggtg cagccatttc gtcgagatca agtttaggac aaaattttgc
     1381 cagttggtgt taatgttttg ttagaatcag tcacgtaatg tgagcacgat taaagtgaga
     1441 tgtgagcaaa tggctaataa accttcggca gaagaactga aaaaaaattt gtccgagatg
     1501 cagttttacg tgacgcagaa tcatgggaca gagccgccat ttacgggtcg tttactgcat
     1561 aacaagcgtg acggcgtata tcactgtttg atctgcgatg ccccgctgtt tcattcccaa
     1621 accaagtatg attccggctg tggttggccc agtttctacg aaccggtgag tgaagaatcc
     1681 attcgttata tcaaagactt gtcacatgga atgcagcgca tagaaattcg ttgcggtaac
     1741 tgtgatgccc atctggggca tgtcttccct gacgggccgc agccaacggg cgaacgttat
     1801 tgtgttaact ctgcttcttt acgcttcact gatggcgaaa acggcgaaga aatcaacggt
     1861 tgaaaaaacg attcagcaac ttattccaca ggagcggaaa aattatgaat cttgatgaca
     1921 ttatcaacag catgacgcct gaggtatacc agcgtttgtc gaccgccgtt gaactgggga
     1981 aatggcccga tggcgttgcg ttgaccgagg aacaaaaagc gaactgcctg caactggtga
     2041 tgctgtggca agctcgccat aataccgaag cacagcatat gacaattgac acaaatggtc
     2101 agatggtcat gaagagcaaa cagcagttaa aagaagattt tggtatctca gcaaagccta
     2161 ttgctatgtt taagtgaatg agttatctag tatttaagcc tgttattaag ggagggcaaa
     2221 tatacttgtt aggacgtcgt gttttagctg caagtaggtt aaatgcaaca ctgacaagta
     2281 ttatctggcc tacaatgtca ttgaggaaat gtaggccaga gatgaattca actaatatta
     2341 cccctgtgtc tcttcccagt ctgccagcgt atatagcgtt gccccagctg ctgacatctc
     2401 cataaacgcg tgcgcactgt cctggggctg gatattcacg ccacgacaac catcggtaat
     2461 cacatttacc ttataaccta actgtaacgc gtccagcacg gtaaacttca cgcaatagtc
     2521 agtagccagg cccataacga tcaattcatc gatttcatga tcgcgtaacc agtcatcgag
     2581 cgaggttttc tgccgacggc cgttatcaaa aaaggcactg taactgtcaa ctaaaggatt
     2641 ttcgccttta tggaacaccg ctgcgatcgc tttttggttc agtaacggat gtaattgtgc
     2701 gccttcactg ttctgcacac agtgatctgg ccagaaggtt tgtggcaaac cgtcgagttg
     2761 gcctggcgta taaggctcta caccgtgctg actggcaaaa ctgccgtgat tcgccgggtg
     2821 ccagtcctga ctggcgataa ccgcttcacc gcgcgactgg caccagtcaa tcaggcggtt
     2881 agcgacatcc accgtactgt caccttccgg cacggcgagc gcgccaccag cacagaaatc
     2941 attttgtaaa tcgaccagta acagggcgcg agggggcatt acagtctcct taatcatccg
     3001 gcgtcagttc gccgcgcagg ttttggctca tggccttgcg aatggtttca gtatccagtt
     3061 cctggctcag caggtaatgc agtttggtta gcgtggcttc tacagtcata tctgcaccgc
     3121 caataacgcc ggcatgggcg agggcgttac cggtggcata accacccata ttcactttac
     3181 cggacataca ttgtgtcagg ttgaccacca caataccgcg atcgctggct tcttgtaatt
     3241 cctgcaggaa ggctttgttt tgtggcgcat tacccacgcc ataggagcgc agaatcaatg
     3301 ctttcaccgg ttggcgcaga aaattgcgca cgacgtcagc agaaatccct ggataaatcg
     3361 tcactacgcc aattggttgt ggcgtgattg gatgaacgat taattcccct tcaccgtgcg
     3421 gggcgggtgg cgtattcaaa cgacgaatat ggataccagc ttccagtaac ggaggaaggt
     3481 ttggagaggc aaacgcatca aaaccatcgg catgggcttt ggtagtgcgg ttgccgcgat
     3541 acaatcggtt attgaaaaag agcgttactt cgttgatcgg ataattcgcc gcaacgtaca
     3601 acgcattcag cagattaatt tgtccgtcag agcgtaactc agccagcggg atttgtgacc
     3661 ctgtcacaat gaccggttta ccgagattct cgagcatgaa cgacagcgca gaggcagtat
     3721 acgccatcgt gtcggtgccg tgcaggatga caaaaccatc atagtcgtca tagtgcgctt
     3781 taatatcttc agcaatatgc tgccagtctt ctggcgtcat atcagaagaa tccatcagcg
     3841 gcgtatattc atgaatggtg aaatctggca tctccgggcg atggaattcc ggcatcagcg
     3901 ccagttggcg ttgtagatga cctgacaccg gaatataacc ctgctcggaa cgctgcatcc
     3961 cgatggtccc gcccgtgtag gcgacgtaaa ttgatttctt ttgcatgata ttgatatgaa
     4021 tgatatcgaa agagcaaaag tatatacgtg aggcaggagg agcgcacctg atactatgcg
     4081 gataaggaaa tatggggcga gacagcggga agaggattcg tcgacgtaaa aaataatgtg
     4141 gccgacgatc tgtcggccac tcagtacaag acttaacgca cgttggcgca ggtcaggcaa
     4201 aacgcataac ggttttgtgg gtcgttaaac gcggccagct tgtcgctttc acttttaacg
     4261 gtagaggcta ccgaggcaag cggtgcaggt aacatggcct ggaacgcatc tggcaacatt
     4321 gcccggacag aaccagacat gttgtccatc actttgtcga agaaggtcgg ttcatcaacg
     4381 tagtattcca gatgccactg tttcactttt gccagctctg ctgctttggc gaccgcatca
     4441 tcgaaatccc cgagactatc gaccagcccg ttagcttttg catcctgacc ggtccagacg
     4501 tggccctggg cgattttatc aatctgctcc ggcgtcgaat gacgcgcatc agcaaccagc
     4561 gtgataaagc gtttatagcc attctcaatg cttaattgca tcatctgctg cgcttccggc
     4621 ggcagtgccc tggtgataga aacatccgcc agcggtgaag ttgagacacc atcagtatga
     4681 acaccaatcg aatccagact attttctacg gtggtgatca cgccgaagat accgatagaa
     4741 ccggtcaggg tgctggggtt agccacaatg taattagctg gcgtggaaat ccagtaacca
     4801 ccagatgccg ccatgccgcc catcgataca accacaggct tacccgctgc ccgggctgct
     4861 gccagttcag cgcgaatcac ttcagacgcg gtaacgctgc cgcctgggct attaacacgc
     4921 aggacaatcg ctttcacttt cgggtcaagg cgagcgtcgc ggatttgtgc cgcagtggta
     4981 tcaccgccaa cattcccctg agtttcctcg ccatccataa ttgcgccatt agcgaagacg
     5041 acaccgatgc tgtcaccggt atctgccggc gttttcaatg cgtaatcgta ataactgatg
     5101 gcgcgataat ttttatcagt cttactccag ccgaactctt tggttagagc tttttcgatt
     5161 tcggcactcg atgccagtgc atcgaccagc ttgttttcca gtgcatattt cgcggtatcg
     5221 ccaccggttt tggttaaacc ctcaagcaac ccttgcgcgc cagggaatac ctgctgagca
     5281 gggatctgcc ggttagcggc aacagtattc agatagtttt gccacagctc accaatccag
     5341 cggctgtcag cttcgcgggc tgccggtgac atgtcatcac gaataaacgg ttcaacggca
     5401 gatttatacg tacccacgcg gaacacatgg gtggaaactt tcagcttatc cagcaacgat
     5461 ttgtagtaca gaccgttggt agcaaaaccg tgcaaatcca ccacgccttg cggtgacagc
     5521 caaattttat tggcgaaact ggcgagataa tattgcccct ggctgtagtt ctcgccaacg
     5581 gcataaaccg gtttcccgct gtcacgaaac tctttcaggg ctttgccgat gtactgcata
     5641 gacggctggt cgccgcctgc gaagtttttc agatccatca cgataccggt gatattgcgg
     5701 tcgtccttcg cctggcgaat ggtgttgacg atatcaaaca gtgagttttc ctgcagacga
     5761 tcggaactgg caccaagcag ctggcggctt aatttactaa accgctgaga actgtcgggt
     5821 ttatcgacga tcacaccaga aatgtccagc agcagtgcgc cacgactggc cgtttctttc
     5881 gaatcaccac cactgacctg catccagatc cccacaccaa ccagtacgag gaaaataaag
     5941 aacaggttaa gtaccatttc acggacgaaa ttcagcagac gccacgtcca tttaaaaaat
     6001 ccggcaataa atcgccaaag ggttcgcatg tattctccca acttaaggtc acacctgtca
     6061 caggcgcaat agatgtggtt atcgtaatga cacaaccggc acttgtcagc aggaattgcg
     6121 gactgtgctg taacaaaatc cactctcgtg ttaattttgt gaataaatat cacgacagga
     6181 gttaatcaaa tggatgcact cgaactattg atcaatcgcc gtagcgcctc ccgcctggct
     6241 gaacccgcgc caacgggtga acaactgcaa aacatcctgc gtgcgggtat gcgtgcgccg
     6301 gaccataagt ccatgcaacc gtggcatttt tttgtgattg aaggggaagg gcgcgagcgt
     6361 ttcagcgccg tactggaaca gggggcgatt gctgccggta gtgatgacaa agctatcgac
     6421 aaagcccgta atgcgccgtt ccgcgcaccg ctcatcatca cggtggtggc gaaatgcgaa
     6481 gaaaatcata aagtcccgcg atgggaacag gaaatgtctg ccggatgcgc ggtcatggcg
     6541 atgcaaatgg cagccgttgc ccagggattt ggcggcatct ggcgcagtgg cgcattaact
     6601 gaaagtccgg tagtgcgtga agcattcggt tgccgtgagc aggataaaat tgtcggtttt
     6661 ctctacctcg gcacgccgca gttgaaagca tctacgtcga ttaacgtccc ggacccgacg
     6721 ccgtttgtaa cttatttctg ataattgtcg cgaaactgtc tggattgtga gcaaaggcgc
     6781 aggaattcag acactctcac ttatcacttc acggaatgag ggttaccata cccggattgc
     6841 attgacagga gatgtccatg agcgagaact cgattcgttt gacccaatac agccacggag
     6901 ctggttgcgg ctgtaaaatt tccccaaaag tgttggaaac catcctgcac agtgagcagg
     6961 cgaagtttgt tgatccgaat ttgcttgtgg gtaatgagac ccgcgacgat gcggcggtgt
     7021 acgatctggg caatggcacc agcgttatca gtaccaccga cttctttatg ccgatcgttg
     7081 ataatccttt cgattttggc cgcattgcgg cgactaacgc catcagcgat atcttcgcga
     7141 tggggggcaa accgattatg gcgattgcga tcctcggctg gccgattaac aaactttccc
     7201 cggaaattgc ccgcgaagta accgaaggtg gacgctatgc atgccgtcag gcgggtattg
     7261 cgctggctgg cggtcactcc atcgatgcga cggagccgat ttttggtctg gcggtaacgg
     7321 ggatcgtacc gaccgagcgg gtgaagaaaa acagtaccgc acaagccgga tgcaaactgt
     7381 tcctgacgaa accgctgggg atcggcgttc ttaccacggc tgagaaaaaa tcactgctga
     7441 aaccagaaca tcagggacta gcgacggaag tgatgtgccg gatgaacatc gcaggcgcgt
     7501 cctttgccaa catcgaaggc gtaaaagcga tgaccgacgt tacgggtttt ggtctgctgg
     7561 gccatttgag cgaaatgtgt cagggcgcag gtgtgcaggc gcgcgttgac tatgacgcga
     7621 tcccaaaatt gccgggggtt gaagagtaca ttaagttggg cgcagtgcct ggcggcaccg
     7681 aacgtaactt tgccagctac ggtcatctga tgggtgaaat gccgcgtgaa gtgcgcgatt
     7741 tgctgtgcga tccgcaaact tctggcggtt tgctgctggc ggtcatgccg gaagcagaaa
     7801 atgaggtcaa agctacagcc gccgagtttg gcattgaact gacggcaatt ggcgaactgg
     7861 tgccagcgcg cggcggtcgt gccatggttg agattcgtta attcaatgcg gttgtttatt
     7921 gccgaaaaac cgagtctggc gcgcgccatt gctgatgtcc tgcccaaacc gcaccggaaa
     7981 ggcgatggct ttatcgagtg cggtaatggt caggtggtga cctggtgtat cggtcacctg
     8041 cttgagcagg cgcagccaga cgcctacgac agccgctatg cgcgctggaa tcttgcggat
     8101 ttgccgatta tcccggaaaa gtggcaatta cagccccgac cctccgtgac caaacaactt
     8161 aacgtcatca aacgcttcct gcatgaagcc agcgaaatcg ttcacgccgg ggacccggat
     8221 cgtgaagggc aactgctggt ggatgaagtg ctggactatc tgcaactggc accggaaaag
     8281 cgccagcagg tgcagcgttg cttgataaac gacctgaacc cgcaggcggt tgagcgggcg
     8341 ctcgaccgtc ttcggtccaa cagtgagttt gtgccattgt gcgtttctgc gctggcgcga
     8401 gcgcgtgccg actggctgta cggcatcaat atgacccgtg cgtataccat tctcggtcgc
     8461 aatgccggtt atcagggcgt actttccgtg ggacgcgtgc agacacccgt gctcgggctg
     8521 gtggtgcgcc gcgatgaaga gattgaaaac ttcgtggcga aagacttctt tgaagtcaaa
     8581 gcacatatcg tgacacctgc cgatgagcgg tttaccgcta tctggcaacc gagcgaagcg
     8641 tgtgaaccgt accaggatga agaagggcgc ttgttacatc gtccactggc ggagcatgtg
     8701 gttaaccgca ttagtggtca accagcaatt gtcaccagct ataacgataa acgggaatca
     8761 gaatccgcgc cgctgccttt ttcgctttcg gcgttgcaga ttgaagcggc aaaacgcttt
     8821 ggtctgagcg cgcagaacgt gcttgatatc tgccagaagc tgtacgaaac gcacaagcta
     8881 atcacttatc cgcgttccga ttgtcgctat ttgccagaag aacattttgc cggacgccac
     8941 gcggtgatga atgccatcag cgttcatgca ccagatctgt tgctgcagcc agtggtagat
     9001 ccagatatac gcaaccgctg ttgggatgac aaaaaggtcg atgcacacca cgccatcatc
     9061 ccgaccgcac ggagttcagc catcaacctg acggagaacg aagcgaaggt ctataacctg
     9121 attgcccgtc agtacttgat gcagttctgc ccggatgtgg tgttccgcaa gtgtgttatc
     9181 gaactggaca ttgccaaagg caaatttgtc gctaaagcgc gttttcttgc tgaagcaggc
     9241 tggcgcacgc tgttaggcag caaagagcgc gatgaagaaa acgacggtac gccattgcct
     9301 gtggtggcga aaggcgatga gttgctgtgt gaaaaaggtg aagtggtaga gcggcaaacc
     9361 cagccgccgc gccattttac cgatgcaaca ctgctttcgg cgatgaccgg gatcgcgcgc
     9421 tttgtgcagg acaaagatct gaaaaagatc cttcgtgcga ccgatggtct ggggacagaa
     9481 gcaacccgtg ccgggattat tgaactgctg ttcaagcgtg gttttctgac caaaaaaggg
     9541 cgctatatcc actctaccga cgccggaaaa gcgttattcc attcgctgcc agaaatggcg
     9601 acgcgaccgg acatgaccgc gcactgggaa tcggtgctga cgcaaatcag cgaaaagcag
     9661 tgtcgctatc aggactttat gcagccgctg gtggggacgc tatatcagct tattgatcaa
     9721 gccaaacgta cgccggtgcg gcagtttcgc ggcattgtgg ctccgggcag tggtggcagt
     9781 gctgataaga aaaaggctgc accgcgtaaa cgtagtgcga aaaaaagtcc gccagcagat
     9841 gaagtcggaa gcggggcgat agcgtaagcg agtgaatctt tcgtgctatt cgagtc
//
LOCUS       DAZSBF010000150         9851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZSBF010000150 DAZSBF010000000
VERSION     DAZSBF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.152"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHXWD_002976"
     CDS             complement(<1..163)
                     /locus_tag="ACHXWD_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR7621834.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHXWD_002977"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHXWD_002977"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR7621835.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHXWD_002978"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHXWD_002978"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR7621836.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHXWD_002979"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHXWD_002979"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR7621837.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHXWD_002980"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHXWD_002980"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR7621838.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHXWD_002981"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHXWD_002981"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR7621839.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHXWD_002982"
     CDS             5058..6191
                     /locus_tag="ACHXWD_002982"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR7621840.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHXWD_002983"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHXWD_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR7621841.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHXWD_002984"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHXWD_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR7621842.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHXWD_002985"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHXWD_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR7621843.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHXWD_002986"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHXWD_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR7621844.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHXWD_002987"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHXWD_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR7621845.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHXWD_002988"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHXWD_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR7621846.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9851
                     /gene="yjeJ"
                     /locus_tag="ACHXWD_002989"
     CDS             9762..>9851
                     /gene="yjeJ"
                     /locus_tag="ACHXWD_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR7621847.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2505 c         2502 g         2327 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg c
//
LOCUS       DAZSBF010000151         9808 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZSBF010000151 DAZSBF010000000
VERSION     DAZSBF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.153"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(57..1114)
                     /locus_tag="ACHXWD_002990"
                     /pseudo
     CDS             complement(57..1114)
                     /locus_tag="ACHXWD_002990"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(1205..1900)
                     /gene="rsuA"
                     /locus_tag="ACHXWD_002991"
     CDS             complement(1205..1900)
                     /gene="rsuA"
                     /locus_tag="ACHXWD_002991"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR7621848.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            2050..3810
                     /locus_tag="ACHXWD_002992"
     CDS             2050..3810
                     /locus_tag="ACHXWD_002992"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR7621849.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            3935..4219
                     /gene="rplY"
                     /locus_tag="ACHXWD_002993"
     CDS             3935..4219
                     /gene="rplY"
                     /locus_tag="ACHXWD_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR7621850.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(4358..5365)
                     /gene="yejK"
                     /locus_tag="ACHXWD_002994"
     CDS             complement(4358..5365)
                     /gene="yejK"
                     /locus_tag="ACHXWD_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR7621851.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            5547..5774
                     /locus_tag="ACHXWD_002995"
     CDS             5547..5774
                     /locus_tag="ACHXWD_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR7621852.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            5794..7554
                     /gene="yejM"
                     /locus_tag="ACHXWD_002996"
     CDS             5794..7554
                     /gene="yejM"
                     /locus_tag="ACHXWD_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR7621853.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            7629..7705
                     /locus_tag="ACHXWD_002997"
     tRNA            7629..7705
                     /locus_tag="ACHXWD_002997"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7663..7665,aa:Pro,seq:ggg)
     gene            complement(7808..9808)
                     /gene="yejO"
                     /locus_tag="ACHXWD_002998"
                     /pseudo
     CDS             complement(7808..9808)
                     /gene="yejO"
                     /locus_tag="ACHXWD_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         2440 a         2645 c         2376 g         2347 t
ORIGIN      
        1 tgtggttgac atgcatcaac aaaggaagcc ttttagcttc ctcgttgtgc aatagatcac
       61 cgttttttcg gtcgactggc gtacagacag aagagaatgg agctggttgc gcagaatgca
      121 attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa gagaaagcaa tgcgccaaca
      181 attgccccta tgccaaaacg gaaggttcct gccagcgaag atgccgttcc cgccatatgg
      241 ggaaactcat caagaatgac cgccatcgca ttggatgaca ccatagacac gcagcccaca
      301 aacgccgcaa cgccaaccac cagcgaccag aatcccagcc ccagcagcgc actgatgacc
      361 atccacgctg ccataataaa ttgtatccac aaccccgagc ggaacatatt taacgcgcca
      421 atgcggcgga cgaagcggct gttaaagatg gtcatcacga acagaaaaac aatgtttagc
      481 gcaaagtaat aaccaaagtt ttccggtgca acgtggttga tttcaatgta gacaaacggt
      541 ccggcgctca ggaacgagaa cattccggca aagctgaaac cgctggccag catataactc
      601 aggacacgct tatgacggaa cagcgccgca aagttaccaa tagtggtacg aatgtgaaaa
      661 ggctggcgag gatccagaag atgtaatgcc agctcagcca caccagcacc cagccgccaa
      721 ctatcggtgc catcagcggt gcaatggtcg tcaccagcat gacaaacgac atcatccgcg
      781 agaactcttc tttcgggtaa atatcacgca tcaaagcgtt gataaccacg ctggccgcag
      841 ccgcagccag cccgtggaag aaacgcatca caatcagctg atcgatggtt tgtgccaacg
      901 cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc aagaaccacc ggcttacgcc
      961 cgaagctgtc tgccatcggc ccgtagatta actgccccaa cgcaaagccc agaatataag
     1021 tactcagggt catctgcgta ctgcccgccg gtacgccaaa ctgcgctgaa attaccggta
     1081 gcgcgggcag atacatatca atcgacagcg gcatcaacat ggccagcagg ccaaggataa
     1141 aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac gggctcctga aagtcattga
     1201 aaagttagac gacgctggca atttcttctt cagttaacgg acgatattca ccgggggcta
     1261 aatcagcatc cagcgtaatg ccgccaatac gttcacgatg cagctcaacc acatggttac
     1321 ccacggcggc gaacatgcgt ttcacctgat gataacgccc ttcgctgatg gtcagacgaa
     1381 cctgcgttgg ggtaatcact tccagcaccg ccggcttagt gagatctttt tcgttatgca
     1441 gctgcacgcc tttagcaaat tgttctgccg tatcgtcagc tacgggtgat accagcgtca
     1501 ccagataggt cttctcgcaa tgatggcgcg gagaagtaat gcggtgcgac cactgaccat
     1561 catctgtcat tagtaccaga ccggtggtat cgatatccag ccgccctgcg gcgtgcagtt
     1621 tccacgctac cggttcatct agaaaataga gcaccgttgg gtgatcaggg tcgtccgtgg
     1681 agcaaacata accctgaggc ttattgagca tgaagtaacg tggaccgtgt tgctgcgcca
     1741 gcgggttgcc atcgtaagcg acatcatgtt caggaagcag tttgaacgct gcattacgga
     1801 cgatttcgcc atcgacggtg acacgattgc cgcggatttc acgcccggca atagcacggc
     1861 taacgccgag ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt ttgcctgtaa
     1921 aaaacggagg tcgggtaatg acccgaatat ctgaactgtt gtctgcccag tatagcggtc
     1981 taataacatc cctcaaggga aaaagatccg tggcatacta ttagcagaat aatctaccta
     2041 cgcgagacca tgatttttac acttcgccca tatcagcaag aagccgtgga tgccacgctc
     2101 aaccattttc gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg tgcaggtaaa
     2161 agcctggtga tagcggaact ggcacggctg gctcgtggtc gcgtactggt gctggcacac
     2221 gttaaagaac tggtggcgca aaaccatgca aagtatcagg cgctggggct ggaagccgat
     2281 atttttgccg ccgggctgaa gcgtaaagag agccacggta aagtggtatt tggcagtgtg
     2341 cagtcggtcg cccgtaatat tgatgccttt cagggtgaat tttcgctgtt gattgtcgat
     2401 gaatgtcacc gtattggtga cgatgaagag agccagtatc agcaaatcct cactcacctg
     2461 acaaaagtga atccccactt acgcctgctg gggctgactg ccacgccttt tcgattgggc
     2521 aaaggttgga tctaccagtt ccattatcac ggcatggtac gcagcgatga gaaagccctt
     2581 ttccgtgact gcatttatga gctgcccctg cgttatatga ttaaacacag ctatctgacg
     2641 ccgccagaac gactggatat gccagtagtg caatatgatt tcagccgctt gcaggcacag
     2701 agcaacgggc tgttcagcga agccgatctc aaccgtgagc tgaaaaaaca gcaacgtatt
     2761 accccgcaca tcatcagcca gattatggag ttcgccgaaa agcgtaaagg agtgatgatt
     2821 ttcgccgcga cggtcgaaca cgcaaaagag attgtgggat tactgcctgc cgaagatgca
     2881 gcactgatta ctggcgacac ccccggcgct gagcgcgatg tgttaattga aaattttaaa
     2941 gcccagcgtt ttcgctatct ggtcaacgtc gcggtactga ccaccggatt tgacgccccg
     3001 cacgtcgatc ttatcgccat tctgcgccct accgaatcag tgagtcttta ccaacaaatt
     3061 gtcgggcgcg gtctgcgtct cgctccgggc aagactgatt gtttaattct tgattatgcg
     3121 ggtaatcctc acgatctcta cgcgccggaa gttggtacac cgaaaggcaa aagtgacaac
     3181 gttccggtac aggttttctg ccctgcctgc ggttttgcca acaccttttg ggggaaaacg
     3241 accgccgacg ggacattgat tgaacacttt ggtcgccgct gtcagggatg gtttgaagat
     3301 gacgacggtc atcgcgaaca gtgtgacttc cgtttccgtt ttaaaaattg cccgcaatgt
     3361 aacgcagaaa atgatattgc cgctcgccgc tgccgcgaat gtgacacggt actggttgat
     3421 ccggatgata tgttaaaagc ggcgctacga ctgaaagatg cgctggtatt acgctgtagc
     3481 ggcatgtctt tgcagcatgg gcacgacgag aaaggcgaat ggttgaaaat cacctattac
     3541 gatgaagacg gtgcggatgt gagtgagcgt ttccgtctgc aaacacctgc ccagcgtacc
     3601 gccttcgagc agctttttat ccgcccgcat acgcgcacac cgggcatccc gctgcgctgg
     3661 atcaccgccg ccgatatcct cgcccagcaa gccttattgc gacccccgga ttttgtcgtc
     3721 gcccgcatga aaggccagta ctggcaggtg cgtgaaaaag tgttcgatta cgaaggtcgt
     3781 tttcgtcggg cgcacgaatt acgcggttaa tggtagtttt gattgatgta taaggcgatt
     3841 gagtatagaa tctcacccgc ttttgcatac gcaaagcaga tcacttacct gttgctgggt
     3901 cgcctgtagc aggattaatt taagagagaa agaaatgttt actatcaacg cagaagtacg
     3961 taaagagcag ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt tcccggcaat
     4021 catctacggt ggcaaagaag cgccgctggc tatcgagctg gatcacgaca aagtcatgaa
     4081 catgcaagct aaagctgaat tctacagcga agttctgacc atcgttgttg acggtaaaga
     4141 aatcaaagtt aaagctcagg acgtacagcg tcacccgtac aaaccgaagc tgcagcacat
     4201 cgacttcgtt cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc cgcggggttt
     4261 tttgtatctg cagattatgc ctgattacgg tattgctatt ttttgcaggc cagataaggc
     4321 gttcacgccg catctggcat tcagaacaca aaaccgatta attgccgcca gacgtccggc
     4381 gctgcaattg gtcgcgcaaa ttcggcggcg tgcctttaat ggtcagggta tcggtcgccg
     4441 gctcccagaa gatgcgttca cctaacagca tcgcatcaaa gttaatagtc aagccaccgc
     4501 cgctaccggc aaacttcgtc aactgacgca gtgtgctccg gtctgccggg aagctctctt
     4561 ccagttcgta gcctttttca gccgcaaact ccgtgaagct cacttcgctt acgcctgcca
     4621 gctctttcga cagcgattcc agttcgatct cttcgcctgc ctgcagctgc tcattacagt
     4681 agctataaac ctgctggcgc acgttctgtc gctctgcttt atccagttgt gcttcggcag
     4741 tgaaatcatc aaccgcttgc aacagcccac ggttttgtgc tttcgtgttc aatccttcgc
     4801 tggccccgag gaaatccata aagaaatccg ccactttgcg ccctactcgc cctttcagga
     4861 aagtgagata gcgggtggac tctggattgg tttcccattc ggttaaatcg atacgcgcaa
     4921 caatatccgc atgattgata tcaagataat gggttgggtt gatgtcgaga ttttcgttaa
     4981 cacgcatact gctcaggttg ctcagaactg ccaccagcag atactccacc gccagataac
     5041 ggtagtggca aaacagcacg aatccaccgt cagcaaaagg atatttcgct aattcgtcac
     5101 gcaggcgacc agttgccgcg cggctaaatg ccagaaagtc ctcttcgccc tgacgctgta
     5161 agcgcagcgt ctgcgccagt tcactctctt cgctaaacaa gccgtaagct ttatttttgg
     5221 cgctatagac ccgatgcagt tctgccacca tctcaacgac ggtttctgtc ggttccagca
     5281 atgaatcgcg caacaccagc tcaaggttct gctcatcacg cttgataagc tggtgcagag
     5341 caatctggtt gatatccaga ctcatgataa actctccttt aagaccgggc ggtattcaac
     5401 caccgccggt taagcgacgc aataaaagat ggcggtcatt ttacagaagg cagcgtaacg
     5461 agaatgattt aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa tatgttgccc
     5521 tttcatcaac taactgattt cgatttatgc cacaaatttc ccgctacagt gatgaacagg
     5581 ttgaacaatt gctcgcagag ctgctcaacg tactggaaaa acataaggct ccgaccgacc
     5641 tttccctgat ggtgttaggc aacatggtca ctaaccttat caacaccagc attgccccgg
     5701 cccaacgcca ggcaattgcc aactcttttg cccgcgcctt acagtcctct atcaacgaag
     5761 acaaagcgca ctaagggaaa cagataacag gttatggtaa ctcatcgtca gcgctaccgt
     5821 gaaaaagtct cccagatggt cagttggggg cactggtttg cactgttcaa tattctgctt
     5881 tcgctcgtca ttggcagccg ttacctgttt atcgccgact ggccgacaac gcttgctggt
     5941 cgcatttatt cctacgtaag cattatcggc catttcagct tcctggtgtt cgccacctac
     6001 ttgctgatcc tcttcccgct gacctttatc gtcggctccc agaggctgat gaggtttttg
     6061 tccgtcattc tggcaacggc gggaatgacg ctattactga tcgatagcga agtctttact
     6121 cgtttccatc tccatcttaa tcccatcgtc tggcagttgg ttatcaaccc agacgaaaat
     6181 gagatggcgc gcgactggca gctgatgttc atcagcgtgc cggttatttt attgcttgaa
     6241 ctggtgtttg cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg tcgacgcttc
     6301 gcgcgcccgc tggccgcatt cttatttatc gcctttatcg cctcgcatgt ggtgtatatc
     6361 tgggccgatg ccaacttcta tcgcccgatc accatgcagc gcgctaacct gccgctttcg
     6421 tacccgatga cggcgcgacg ttttcttgag aagcatggtc tgcttgatgc gcaggagtat
     6481 caacgccgtc ttattgagca aggtaatcca gacgccgttt ccgttcagta tccgttaagc
     6541 gaactgcgct atcgcgatat gggcaccggg cagaatgtgc tgttgattac tgtcgatggc
     6601 ctgaactact cacgcttcga gaagcagatg cctgcgctgg caggttttgc tgagcaaaat
     6661 atttcgttca cgcgccatat gagctccggc aacactacag acaacggcat ctttggcctg
     6721 ttctatggta tctcgccgag ctatatggac ggcattctgt cgacccgtac gcctgcggca
     6781 ttaattactg cgcttaatca gcaaggctat cagctggggt tattctcatc agatggcttt
     6841 accagcccgc tgtatcgcca ggcattgttg tcagatttct cgatgccgag cgtacgcacc
     6901 caatccgacg agcagaccgc cacgcagtgg atcaactggc tgggccacta cgcacaagaa
     6961 gataaccgct ggttctcgtg ggtttctttc aatggcacta acattgacga cagcaatcag
     7021 caggcatttg cacggaaata tagccgggcg gcaggcaatg tcgatgacca gatcaaccgc
     7081 gtgctcaatg cactgcgtga ttctggcaaa ctggacaata cggttgtgat tatcactgcc
     7141 ggtcggggta ttccgctgag cgaagacgaa gaaacctttg actggtccca cggtcatctg
     7201 caggtgccat tagtgattca ctggccaggc acgccggcgc agcgtattaa tgcgctgact
     7261 gatcataccg atctgatgac gacgctgatg caacgcctgc tacatgtcag cacacctgcc
     7321 agcgaatatt cgcaaggtca ggatttgttc aaccctcaac gccgtcatta ctgggttact
     7381 gcagcggata acgatacgct ggcaattacc accccgaaaa agacgctggt gctgaacaat
     7441 aacggtaaat accgcactta caacttacgt ggtgaaagag tgaaagatga aaaaccacag
     7501 ttaagtttgt tattgcaagt gctgacagac gagaagcgtt ttatcgctaa ctgattaatt
     7561 ataaatcagt tagcgaaata tcttacttgc aatcggtgtg gaaaacggta gtattagcag
     7621 ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc gggggtcgga
     7681 ggttcaaatc ctctcgtgcc gaccaaaaat cccaagaaaa aaccaacctt tacggttggt
     7741 ttttttatat ctgcaattaa tttgataaac agaccgtgac acatcacagc ctgtttattt
     7801 tctgttatca gaacgtccag accactcccg cctgagtatt ccacggcgat tctacccctg
     7861 cgccattgcc atagctgact gacaaatgac cgcttaacgt cggggtaaac gatgacctta
     7921 tcccagcctg ataaacgcca cgggttccag ccacatcatt attaaatttg ccatcgtcat
     7981 taactttcac atggttagaa tcggcgtatt cctgacgcac ggcggctttc agccagggtt
     8041 ccaacgtcgt accgttttgc aggtccatgt gatagcttac cgccgttccc gcttcagcgc
     8101 gtaatatccg ggtatttccc acatccgcgc gcatgccgtt tgataacgtg tagtcctgac
     8161 catctgtggt aaagccggta aaggcgagat agggtctaac actccacaat ccgtcaaccc
     8221 aacggaaccc gctctcaaca tgagcacccg cgccgttact attgtaatcg ccaaacgctg
     8281 ttgccccatt actcatcttg ccatggatgg tgttggcaaa acggtcaact ttcaccaccc
     8341 catcaacata ggcaccgttc tgatgctccc aaccggcata agcccccagg gtgtagctat
     8401 caatattgcc tttgccgccg cgatcaaaac caatatcaga atgagagtaa tcaaagaaca
     8461 agccgcgaat tgtgctgctt tcttcacggg agaaacggct atcgataccg agcgtcaggc
     8521 ccgtcaatgt ttgctcaaag ccagctcccg catcagtggt cacgttgttg cgggtgttaa
     8581 ttgccgaact ccacatcgcc gtatcgtaat taacgccttt tacgctacca agacgctcac
     8641 gcacggtgtc cagttctgca tcaaatacca gcggttgtgc tgctgccatg ttcagcacat
     8701 cagtggttga aggggtaatt tgcgcgcgat tctctgcgag gctccagcta tggttgccat
     8761 tatccagcaa ggtatattca tacgtaccga tatcaacaac gcctccggca ttgcccaacg
     8821 tgaatgcagc atcaccgccg cccgtcgtta ccagtgtaag gctatctcct gctgccgggc
     8881 tggcccccgt gtccgtcacg aatattttga aatcacctgt tgcctgaccg atgacgttga
     8941 gctgatcgct ctgatgatta gccatatcgg tacgcatata aaattgccgt ttcccgacag
     9001 ggtattggtg gtcaacgtaa tgaaattttc cgcacttgcc gtcacagatg aacgagtcac
     9061 tggctcagcc atcataatca catttgcgcc ggacatcgca aggttggcta agttgaggcg
     9121 agtgttttgg ttcgtcgtag tgatatcggc aatgatggtc catgcgctat tttcgagcga
     9181 aacgtccgct tggctggtat ccacggcacc atgcgttcta aaagaagcgc cctcagaaac
     9241 cactacctta cccaccaaca tagcgggtgc ataatttatt ttcggcttca tgacctcaag
     9301 aatgccatca ttccctctga ctgacacgtt aaccattgtg ccagcccaga cattagcgcg
     9361 gccattgttg ataaccatat tttcagagat ggctttagag gaatacgtga tacttccatt
     9421 ctgatctgat cgaccgagat catatacagc atttgcctgc actttcgtac cagtatcctt
     9481 tcccagcgat tgcatagtgg catgctcatc aaggatagtg tcaatggcct gagtgtcctc
     9541 cataacaatg agcccggagc catcatccag ttcataattt tttgacacac catcctttat
     9601 actaaattgg cctttactgt ttgtaccact cacttccagc gcattcgttg agacaattaa
     9661 tttcccgcct gcttttttat caacgccagt tgcagtcccg ccatccataa cctccagtaa
     9721 tcctcccttt tcgatagcgg caaagacggc aatcccatct ttttcaacaa ttaagcgccc
     9781 gcctttaatc gtgggatcat tcgccgcg
//
LOCUS       DAZSBF010000152         9644 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZSBF010000152 DAZSBF010000000
VERSION     DAZSBF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.155"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHXWD_002999"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHXWD_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR7621854.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHXWD_003000"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHXWD_003000"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR7621855.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHXWD_003001"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHXWD_003001"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR7621856.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHXWD_003002"
     CDS             complement(5698..7014)
                     /locus_tag="ACHXWD_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR7621857.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHXWD_003003"
     CDS             complement(7004..8761)
                     /locus_tag="ACHXWD_003003"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR7621858.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9644)
                     /locus_tag="ACHXWD_003004"
                     /pseudo
     CDS             complement(8777..9644)
                     /locus_tag="ACHXWD_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAZSBF010000153         9623 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZSBF010000153 DAZSBF010000000
VERSION     DAZSBF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9623
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.156"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..2200
                     /locus_tag="ACHXWD_003005"
                     /pseudo
     CDS             1..2200
                     /locus_tag="ACHXWD_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2193..3329
                     /locus_tag="ACHXWD_003006"
     CDS             2193..3329
                     /locus_tag="ACHXWD_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR7621859.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3326..5326
                     /locus_tag="ACHXWD_003007"
     CDS             3326..5326
                     /locus_tag="ACHXWD_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR7621860.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5448..5909
                     /locus_tag="ACHXWD_003008"
     CDS             5448..5909
                     /locus_tag="ACHXWD_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR7621861.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5950..6420)
                     /locus_tag="ACHXWD_003009"
     CDS             complement(5950..6420)
                     /locus_tag="ACHXWD_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR7621862.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6467..7186)
                     /gene="btsR"
                     /locus_tag="ACHXWD_003010"
                     /gene_synonym="yehT"
     CDS             complement(6467..7186)
                     /gene="btsR"
                     /locus_tag="ACHXWD_003010"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR7621863.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7183..8868)
                     /gene="btsS"
                     /locus_tag="ACHXWD_003011"
     CDS             complement(7183..8868)
                     /gene="btsS"
                     /locus_tag="ACHXWD_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR7621864.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9090..9155
                     /locus_tag="ACHXWD_003012"
                     /pseudo
     CDS             9090..9155
                     /locus_tag="ACHXWD_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9383..>9623
                     /locus_tag="ACHXWD_003013"
     CDS             9383..>9623
                     /locus_tag="ACHXWD_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR7621865.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWF"
BASE COUNT         2354 a         2531 c         2470 g         2268 t
ORIGIN      
        1 gcggccacga tacgtattga ttgaaggccc ggctgatttt aatgaccggg tagacgaact
       61 gtttttagcc caccagcttc cggtagctat ttacagttat tgccagtatc aggacggtgc
      121 agcccccggg cgtggtgcct ggacgccatt tgctgaattt tcgccggagt ggcaggcgct
      181 acaagccgca cgtcgtattc aggcacaaac ttacttcatc gatttgcctt gctgggcgca
      241 gagtgaagaa gtggacgatt cgcctgatac gcaagaggaa agccaggcct tgctgctgcg
      301 tgccacccgc atggataaca gcgacaccct gtgggatcac ttgttcgaag atgaaagcca
      361 gcaaactgca ttaccctctg cactggcgca ctattttgcc caactgcggg gcgattcccc
      421 cggcgatgca ctcaatcgtc agcgcgaagc ctttatggcc cgctggattg gatgggcgat
      481 gcagcaaaac aatggcgacg tgttagtcgt ctgcggtggc tggcacgctc ctgcactggc
      541 aaaaatgtga cgcgaatgcc cgcaggacat taacacgccg gaattgccct cgctggcaga
      601 tgccgttaca ggttgttatc tcacccccta cagtgaaaag cgccttgatg tgctggcagg
      661 atacctttcc ggtatgcctg ccccggtctg gcaaagctgg tactggcagt ggggcttaca
      721 gcaggcggga gaacaactgc taaaaacggt tcttacccgt ttgcgccagc acaaattgcc
      781 tgcttcgacc gcggatatgg ctgccgctca tctgcatgcg atggcgctgg cacagttgcg
      841 cggacataaa ctaccgttac gcactgactg gctggatgcc atagcaggct cgctgattaa
      901 agaagccctg aacgcgccgt tgccgtggag ctatcgcgga gttattcatc ccgacaccga
      961 tccgattctg ctaacgttga tagacacatt agcgggtgac ggattcggta aacttgcccc
     1021 ttctacgcca caaccgcctc tgccaaaaga tgtcacctgc gaactggaac gtaccgcaat
     1081 ctctcttccg gcggagctta ccttaaatcg ctttaacccc aatgggctag cgcaaagtca
     1141 ggtgttacat cggctggcaa tactggagat cccagggatt gtacgccagc agggaagcac
     1201 actgacactt gcaggcaacg gtgaagaacg ctggaaatta acccgcccgc ttagccagca
     1261 tgcggcattg attgaggccg cctgctttgg tgccacactc caggaagccg cacgccataa
     1321 attagaagcc gatatgctgg acgcgggtgg aatcggcagt atcaccacat gtcttagcca
     1381 ggcggcgtta gcgggtctgg cgtccttcag tcaacaatta ctggagcaac tcacattatt
     1441 aatcgcccag gaaaatcaat ttgccgaaat gggccaggcg ctggaagtgc tatatgcctt
     1501 atggcggctg gatgaaatta gcggtatgca aggcgcgcag atattacaaa cgacgttatg
     1561 cgcggctatc gatcgcacgc tgtggctgtg tgaatctaac ggcagaccag atgaaaagga
     1621 gtttcacgct cacctgcata gctggcaggc gctttgccat atcctgcgcg atctacatag
     1681 cggcgttaat ttacccggcg tttttctttc tgcggcggta gccttactgg agcgccgcag
     1741 tcaggcgatt catgtcccgg cgctggatcg cggcgcggct cttggcgcac tgatgcgtct
     1801 ggaacatccc aacgccagtg ccgaagcggc gctgacgatg ctggcgcagt tatccccggc
     1861 acaatccggc gaggcgctgc acggtttgct ggcgctcgcc cgtcatcaac tggcctgcca
     1921 accagcattt atcgccggtt tcagtagtca tttaaatcaa ctgagtgacg acgattttat
     1981 taacgccctg cccgatttgc gcgcggcgat ggcctggcta ccgccacgag aacgcgggac
     2041 actggcgcat caggtgcttg agcattacca gcttgcgcag cttcctgttt cagcgctgca
     2101 aatgccgttg cattgtcctc cgcaagccat tgcacatcat caacaactcg aacagcaggc
     2161 actggcatcg ctgcaaaact ggggagtttt ccatgtctga actgaacgat cttctgacca
     2221 cccgtgagct acaacgctgg cgattaattc ttggcgaagc ggcagaaacg acgctttgtg
     2281 ggctggatga caacgcccgg cagatagacc acgcgctgga atggctgtat gggcgcgatc
     2341 ctgaacggct ccagcgtggt gaacgctccg gtggattagg tggctcaaat ctcaccactc
     2401 ctgagtggat caacagtatt cacacgctgt ttccgcaaca ggtgattgag cggctggaaa
     2461 gcgatgccgt actgcgctac ggcattgaag atgtggtgac gaatctcgac atgctggaac
     2521 gtatgcagcc ttctgaaagc ctgctacgcg ccgttttgca caccaaacat ctgatgaatc
     2581 ccgaagtact ggctgccgcc cgccggatag tgcgccaggt tgttgaagaa attatggctc
     2641 gactggcaaa ggaagttcgt caggcttttt ctggtgtccg cgatcgccgt cgccgctcat
     2701 ttattccact ggcgcgaaac tttgatttca aaagtactct gcgcgctaac ctgcaacact
     2761 ggcacccgca acacggcaag ttgtatatcg aatccccccg ctttaacagc cgcattaaac
     2821 gccaaagcga acaatggcaa ctggtcttac tggttgatca aagcggatcg atggtggact
     2881 cggtgatcca ctctgcggtg atggcggcct gtttgtggca gttacccggc attcgtaccc
     2941 atctggtggc gtttgacaca agcgtggttg atctcacggc agacgttgcc gatccggtag
     3001 agttattaat gaaagtacag ttgggcggcg ggaccaatat cgccagtgcc gtggagtatg
     3061 gtcggcaact tattgaacaa ccagcaaaaa gcgtcattat cctcgtgagc gatttttacg
     3121 aagggggttc atcatcattg ctgacgcatc aggtgaaaaa gtgtgtccag agcggcatca
     3181 aagtgctggg actggctgcg ctcgatagca ccgcaacacc ttgctatgac cgcgatacgg
     3241 cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat gacgccgggc gagctggcat
     3301 catggcttgc ggagaatctt cagtcatgaa ttcactacgt ccggaattat tagaactgac
     3361 accgcaggcc ctgacggcgt taagcaatgc cggttttgtt aagcgcagtc ttaaggaact
     3421 ggaaaatggc aacgtcccgg agatcagcca tgagaacgac gctttaatcg ccaccttcag
     3481 tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg aaagaggctc agtgcagttg
     3541 cggggccaac ggtatgtgcc gtcatcgcgt gatgctggtg ttaagttatc aacgactttg
     3601 tgccaccact cagtctacgg aaaaagaaga agagtgggat ccggcaatct ggctggaaga
     3661 actggctacc cttcccgatg ctacccgcaa acgcgcacag gcgctggtcg ctaaaggcat
     3721 caccattgag ttgttctgtg cgccgggtga aattccctct gcccgcttac cgatgagcga
     3781 tatgcgtttt tattcccgca gcagtattcg tttcgcccgt tgtgattgta ttgaaagcac
     3841 actttgcgaa catgtcgtac tggcagtaca ggcctttgtc caggcaaaag cgcagcaagc
     3901 ggaattgact catttaatct ggcagatgcg cagcgaacac gtcacatcat ctgacgatcc
     3961 gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt caacaactaa gccaggcatt
     4021 atggctgagc ggcatcagcc agccgcttat tcactacgag gccgctttca gccgcgcgca
     4081 gcaagcagcg gaacgctgca gctggcgatg ggtgagtgaa tcgctacggc aactgcgcgc
     4141 cagcgttgat gccttccacg cccgcgccag ccactatcat gccggagaat gcttacgtca
     4201 gcttgcggca ttaaacagtc gattaaattg cgcacaagag atggcccggc gcgacagtat
     4261 tggtgaagtt cctcctgtgc cgtggcgcac ggtcgttggc tctggcattg ccggagaagc
     4321 aaagcttgat catctgcggc tggtgtcttt aggtatgcgt tgctggcagg atattgagca
     4381 ttatggttta cgcatctggt ttaccgatcc cgacaccggc agtattttgc acctttcgcg
     4441 cagttggccg cgaagtgaac aggaaaactc accggcagct acgcgtcggc tgtttagttt
     4501 tcaggctggc gcactagcgg gcgggcaaat tgtttcacaa gcagcaaaac gcagtgccga
     4561 tggcgagctg ctgttagcta cccgcaaccg cttaagcagc gttgtgccgc tgtcgcctga
     4621 tgcctggcaa atgttgagcg cgccgttacg ccagccgggc attgtggctt tgcgggaata
     4681 tttacgccag cgtccccccg cctgcatacg gcctcttaat caggtcgata acttatttat
     4741 tctgccggtc gctgagtgta tttcgctcgg ttgggatagc agccgccaga cgctggatgc
     4801 gcaggtaatc agcggcgaag gtgaagataa tctcctgacg ttatcccttc cggcatctgc
     4861 ctgttctcct tttgccgttg aacgcatggc ggcgcttttg caacaaacag acgaccccgt
     4921 gagtctggtt tctggctttg tcagctttgt agaggggcaa ttgacactgg aaccacgggt
     4981 gatgatgaca aaaacccgcg cctgggcgct ggacgcagaa actgcgcctg tggcaccgct
     5041 accttctgcc agcgttttgc ctgtgccgtc taccgctcat cagttgctga tgcgctgcca
     5101 ggcgttactt attcaactgc tccataacgg ctggcgctat caggaacaga gcgctattag
     5161 tcaggcagag ttgctggcga atgacctctc cgcggtcggt ttttatcggc tggcacatgt
     5221 gttgggacaa tttcgtaata cagaaagcga ggcacgggta gaagcaatga ataacggtgt
     5281 tttactttgc gaacaattat tccccatgct tcagcaacaa ggatgaaata gtgcttacta
     5341 agagttctac tccagttccg gactgctcac gccacggtat taggcatatc ctatatagcc
     5401 cctggtgaga gtcaccagtt ccttgattaa ataaaatgga gttttacatg aaggctttca
     5461 ataagctgtt ttccctcgtt gttgcatctg ttctggtttt ctctcttgct ggctgcggtg
     5521 acaaagaaga atcgaagaaa ttcagcgcca atctgaacgg cactgaaatt gccattacct
     5581 atgtctacaa aggtgacaag gtgcttaagc aatcttctga aaccaaaatt caatttgcct
     5641 ccattggtgc aaccaccaaa gaagatgctg ccaagacact tgagccgtta agcgccaaat
     5701 acaaaaacat cgcgggtgtt gaagaaaaat taacctatac cgatacctac gcgcaggaaa
     5761 acgtgactat cgatatggaa aaagtggatt ttaaagccct gcagggtatt tcaggaatca
     5821 acgtttctgc tgaagatgcc aaaaaaggta tcactatggc gcaaatggaa ctggtgatga
     5881 aagccgctgg ttttaaagaa gtgaaataat ctgtcggcgg ccatgtttcg catggccgcc
     5941 atacccgtct tagcttttct tcacatgctg gcgcgctgcc agtccacgca gaaaataacg
     6001 taaaaattga tcgccgcatt cgcggaagtt tttatgatcc ggtgcgcgca tcatcgctgt
     6061 aatttccggc atcgaaacgc ggaactgctg ttcggtgagg atcgccagaa tgtcatcggt
     6121 tttcagcgaa aacgcgatgc gtaatttttt cagcacgatg ttgttattaa tgcgacgttc
     6181 cggctccagt gccggagcag actcatcctt gccgcgtttt tcataaatca ggccattgag
     6241 gaatgacgac aaaacaatgt ccggacaacg ctgaaaaccc tcttcgtctt ctttacgtag
     6301 ccagacggcg atctgttccg cggtggcttc gacattaccc agcgccagaa tacgcaccag
     6361 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttagcat
     6421 gaggccttca ggtgttaatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
     6481 cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
     6541 tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca
     6601 gatactgacg atggcagcgc agtagtggtg tacgactttc cagggtacgt aatgtcaatt
     6661 cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga
     6721 caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa
     6781 atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac
     6841 gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
     6901 aggcatgttc ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat
     6961 acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct
     7021 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
     7081 cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat acacgcaggt
     7141 tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
     7201 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata
     7261 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
     7321 attggttacc ggttgataca aaccggcatt gtcttcgatc tccagcatca aatgttgccc
     7381 ctcacgtcgg gcgctgattg ccactcgccc tgtatccagc agttgtgatg tcccatgttt
     7441 aatggcgttt tccactatcg gttgcagggt aaacgcgggc aattgctgct gggataattc
     7501 ttgcggaata gcaatgttga cctgcaaccg cgactggaag cgcgcctttt caatttgcag
     7561 ataagcgttc acatgttcaa tttcgtcggc gagagtaaca aactccgaag gccgctttaa
     7621 gtttttgcgg aaaaaagtgg aaagatactg caccagctgg ctggcctgtt cgctgtcgcg
     7681 gcggatcacc gctttaatgg tgttaagcgc attaaacaaa aaatgaggat tcacctgggc
     7741 gtgaagcagt ttgatttctg actgggtgag catcgctttt tgccgctcat attgcccggc
     7801 aagaatctgc gccgaaagca gttgcgcaat cccctcgccc agcgtgcggt ttattgaact
     7861 gaataaacgg tttttggctt catacaattt gatggtgccc atcacgcgct gattttcgcc
     7921 acgcaacgga attaccagcg tcgaccccag tttgcattgc ggatgcaaag agcaacgata
     7981 aggtacttcg ttgccatcag cgtagaccac ttcaccggtt tcaatcgctt ttaaggtgta
     8041 aggcgaagaa atcggtttgc cgggtaaatg gtggtcgtca ccaattccgg taaaggccag
     8101 caatttctct cgatcggtaa tcgcgactgc accaatatcc agctcctgat acagcacctg
     8161 agccactttc atgctgttca cttcgttaaa cccctgtcgc aaaatgcctt ccgtcgaggc
     8221 tgccactttc agcgcagtgg cagaaaaagc cgaagtgtat ttttcaaaca tcgcgcgttt
     8281 atcgagcaat atacgcataa acagcgccgc gccgacggta ttggtgacca tcattggcgc
     8341 agcaatatta ctcaccagac gcaccgcatc ttcataaggt cgggcgatcg ccaggatgat
     8401 cagcatttgc accatttcag cgacgaacgt gacggcaccg gcggtaatgg ggttaaagac
     8461 tttatcagtg cgcccgcgac ggatcaggat gctgtgtacc aggccgccga gcaatccttc
     8521 aacgatggtt gagatcatgc aacttagcgc ggtcatgccc cccatcgaat atcgatgtaa
     8581 gccgccggtc agaccaacca gcccaccgac gaccggaccg ccgagtaagc cgcccattac
     8641 cgcgcctatc gcacgggtat tggcaataga atcgtcaatg tgcaacccaa accaggtgcc
     8701 catgatgcag aagatggaaa agacgatgta gcagagaaat ttatgcggca gacgaaccgt
     8761 gacctgcatt aacggtatga ataatggcgt tttactcatt aaccacgcaa tgactaaaaa
     8821 aacgcacatc tgctgaagca gcagcaacac cagattaaaa tcgtacatac ccgcaaacca
     8881 cacttccctt taaaacgcgt aacatacatt gcctgcgttt aactttcttt gaactcttgc
     8941 agaaaaatga gaattcgtga gtacgatcac tcaaaatcgc ctggcaaaaa taaaatcacc
     9001 ctatagatgc acaaaaaacg ggcaaaacta cctggttcgc aaaactgcgt ctaaagttaa
     9061 accgggacct cgcgagcaag ggtgagacga tggcgcttta cacaattggt gaagtggcgt
     9121 tgctttgtga tattaaccct gtcacgttac gcgcgtgacg caacattttt aaaaatcgac
     9181 gaagatcgtc caggagcgcc gtttttcaag ggttggatag atgattcgtt taccatgtcg
     9241 acgaacttcg acgaatttcg ccgataaaaa gtgtcccata catgccccat tgatgcccca
     9301 taaacaatat caggaattga cactggttat ccatacagat aaaaataaca cctgtacata
     9361 accacagtta caggagggtg ttatgcgtgt tgagatctgt atcgctaaag aaaaaatcac
     9421 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
     9481 acgttatgac gatgtagagg tgatcgtaaa agccaccagc aacgatggcc tttctgttac
     9541 acgcaccgca gataaggatt ctgcaaaaac atttgttcag gagactctga aagatacctg
     9601 ggagtctgct gacgagtggt ttg
//
LOCUS       DAZSBF010000154         9619 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZSBF010000154 DAZSBF010000000
VERSION     DAZSBF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.157"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..203)
                     /locus_tag="ACHXWD_003014"
     CDS             complement(<1..203)
                     /locus_tag="ACHXWD_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR7621866.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPAGNDSNLLIVFYVQI"
     gene            complement(203..1129)
                     /gene="glsB"
                     /locus_tag="ACHXWD_003015"
     CDS             complement(203..1129)
                     /gene="glsB"
                     /locus_tag="ACHXWD_003015"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR7621867.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1193..2581)
                     /gene="sad"
                     /locus_tag="ACHXWD_003016"
     CDS             complement(1193..2581)
                     /gene="sad"
                     /locus_tag="ACHXWD_003016"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR7621868.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2682..3563
                     /locus_tag="ACHXWD_003017"
     CDS             2682..3563
                     /locus_tag="ACHXWD_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR7621869.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3640..4755
                     /gene="yneK"
                     /locus_tag="ACHXWD_003018"
     CDS             3640..4755
                     /gene="yneK"
                     /locus_tag="ACHXWD_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR7621870.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4905..6095
                     /gene="ydeA"
                     /locus_tag="ACHXWD_003019"
     CDS             4905..6095
                     /gene="ydeA"
                     /locus_tag="ACHXWD_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR7621871.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6120..6785)
                     /gene="marC"
                     /locus_tag="ACHXWD_003020"
     CDS             complement(6120..6785)
                     /gene="marC"
                     /locus_tag="ACHXWD_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR7621872.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6997..7431
                     /gene="marR"
                     /locus_tag="ACHXWD_003021"
     CDS             6997..7431
                     /gene="marR"
                     /locus_tag="ACHXWD_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR7621873.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7451..7834
                     /gene="marA"
                     /locus_tag="ACHXWD_003022"
     CDS             7451..7834
                     /gene="marA"
                     /locus_tag="ACHXWD_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR7621874.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7866..8084
                     /gene="marB"
                     /locus_tag="ACHXWD_003023"
     CDS             7866..8084
                     /gene="marB"
                     /locus_tag="ACHXWD_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR7621875.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8141..9580)
                     /locus_tag="ACHXWD_003024"
     CDS             complement(8141..9580)
                     /locus_tag="ACHXWD_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR7621876.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2485 a         2465 c         2167 g         2502 t
ORIGIN      
        1 attatctgaa cataaaacac tatcaataag ttggagtcat tacctgccgg agcaagacgt
       61 ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac
      121 tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg
      181 aagagtggat cgagagactg cattaataaa ccgaacgtcc taactgtttc gtcaattgtt
      241 caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg
      301 cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg
      361 gtagtcccac ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca
      421 gcgcgttaat ttgccgcgcc tgcattggcg tcactacagg ttcatcaata tgaatagctt
      481 tcccctgatt agccagaaag acaaacgtcc gagccagctc tacacagctc attttcagag
      541 cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg
      601 acttcatcag ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta
      661 ccaccgtatc gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac
      721 gttgccgtgg tgcgcttaat cgcccttgca acatatcgca gaccaccagc gcaccagcat
      781 taataaacgg attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg
      841 gtgatccaga cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca
      901 tggcgacaac gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt
      961 ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc aatgcccaat cgggaaccgt
     1021 ctactgtagc cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca
     1081 cttgccgcaa gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct
     1141 ggaccgcagg tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc
     1201 ggtctttcca caccgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac
     1261 gaccaaagcc actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac
     1321 cattgataaa caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt
     1381 cggtggtaaa aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat
     1441 gttctgcatc tttcgcaacg gtgattgccg caacggggcc aaacatttct tcccgaaacg
     1501 cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgccccag
     1561 ccatcttttc cccgcccagt aacaaacgcg caccctgcgc cagggttttc tctacctgat
     1621 gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc gagagtgttc tcttcgtcac
     1681 ggggatcgcc cattttcaag gctgccgcag ctgccacaaa acgttcggta aatgcagaag
     1741 caattccctc ttcgataata aagcgttttg ccgctgcaca tacctgtccg gtattctgat
     1801 aacgtccggc taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa
     1861 acggatccga accgcccagt tccagtacgc attttttcag tgccgctcca gcctgtgcgc
     1921 caatagccgc tccagcacga acacttccgg tcaccgtgac agcagcaatg cgcgagtctt
     1981 taatcatctg actgacaccg tcgttgtcgg cattcagcca gccatatacg ccttgcggga
     2041 ttcccgcatc tttaaacacc tgggcaatga gctgagcaca tcccatcaca ttcggcgcat
     2101 gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac agcgccacgc atcacctgcc
     2161 ataacggaaa attccacggc ataatcgcca gaatcgtccc caacggtcga tactcaataa
     2221 ccgcctgctg attttccacc agtgtaggtt ccgccttcag cattgccgga ccatgttctg
     2281 cataccagtc acacaaattc gccgatttcg ccacttcagc gcgcgcctgg ttgattggtt
     2341 tgcccatttc gcgggtgatc atttgcgcca tttcttcgct acgggcgcgc agagccttac
     2401 cgatatcacg cattttttca gcacgataat ctatatttgt ctcgcgccag tcgcgaaagc
     2461 ctgctgccgc cagctcaagt gcgttttcga tatcgttagc gccagcccac ggcagcacag
     2521 aaagttgttc acccgtggca ggatttattg aaattgcatg agttgccgga gtaatggtca
     2581 tcggggtatc tcctttatga gtcatggtat gaagatacgc agatttactc ttgctttaaa
     2641 atgaataata ttaagccact tattcacgaa tcgagaatgt tatggatctg acccaactgg
     2701 agatgttcaa cgcggttgcc gaagctggca gcattaccca ggctgcagca aaagtgcatc
     2761 gcgtaccgtc gaatctcact acccgtctac gccagctgga aacagaactg ggggtagatc
     2821 tgtttattcg cgagaatcag cgtttacgtc tctctcctgc cgggcataac tttttacgct
     2881 atagccaaca aattctcgcg ttagtggatg aagcgcggag cgttgtcgct ggcgatgagc
     2941 cgcaaggttt attttctctt ggttcgctgg aaagcaccgc agcagtgcgc attccggcca
     3001 cgctggcgga attcaaccat cgttatccca aaattcagtt ttcactttcc accggccctt
     3061 ccggcactat gctggaagga gtgctggagg gaaaactgaa tgcggcattt attgatgggc
     3121 ccattaacca tactgccatt gacgggatac cggtataccg cgaggagctg atgatcgtca
     3181 cgccacaagg tcatgcgcca gtaatccgcg ccagtcaggt taacggcagt aacatttatg
     3241 ccttccgcac caattgttcg tatcgtcgcc acttcgagag ctggtttcat gctgacggtg
     3301 ccgctccggg aactattcac gagatggagt cgtatcacgg gatgttggcc tgtgtggtcg
     3361 caggagcagg cattgcgctt attccacgct ctatgctgga aagtatgccg gggcatcacc
     3421 aggttgaagc gtggccgtta gctgagcaat ggcgttggtt aacaacctgg ctggtctggc
     3481 gtcgcggtgc gaaaacgcgt ccgcttgagg catttattca actactggat gtgtctgact
     3541 cggcaaaaca gagttaccaa tgaactattt tgataattct tgcggctaat atgctctata
     3601 tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc agtaagcatc
     3661 agcaattaca tatctcttcc tgatgatttt cccgttcgta atattgcacc acaggtaaaa
     3721 gaagttttaa aggactttat tgatgcgcta agtacaataa tatgtgatga agagtggcgc
     3781 acgtctttaa acatcaactc agccacaaaa aagatattta ataaccttga taacttatca
     3841 tatattcaga ggacttcttt ccggggtaac gacacgctat acaatgaaaa ggttcagttt
     3901 aaactcactt atcccgtcaa gaatggaaga cacaaagaaa atattgaatt tcaggtagta
     3961 ataaatttaa gccctattta tttagataat tttcgccatg atggagaaat taatattttt
     4021 tgcgctccca acccaaagcc tgtcactatg gggcgcgtat ttcagaccgg cgtcgagcgt
     4081 gtgctctttc tgtttctgaa tgattttatt gaacaatttc caatgttcaa ccctggcgtt
     4141 ccgatcaaaa tagcgcatac accacatatt gaacccctgc ctccggatca ccataccgct
     4201 gcagattact tacgccagtt tgatttgctt gtcctgaatt ttatctctcg cggtaatttt
     4261 gtcatactcc cccgattatg gaataactct gaggttcaca gatggttcgt caataaagat
     4321 cctaacttga tcaccgccat tctcgacata acggacagcg aattaaaaga ggatttattg
     4381 caaagcctga tggattcatt aggttctaac aaacatgtac tacccgaagt ctgcatctgc
     4441 tttttattcc ttttagcaga acaagaatct cctcattttc aggacttgtt tttatttttc
     4501 gccaatatgt tattgcacta tcaccaattt atgaatccca atgaaagtga tttgaatgac
     4561 gtgttgatgc cagcatcatt aagtgatgat aaaattatca agcatatggc gcgcaggacc
     4621 ctcgaactgt ttgtaaaaaa tgaaacaccg ccaaaagtta ctcacgaaga cctggtgaaa
     4681 aacaggcctc gctcccctgt cagaccacct atacccgcaa cctccaaaac gccagacctc
     4741 cctgaacgtc attaaaccgt gatgttaccg actctctgac gcggaacaga atcagcgtca
     4801 gagaaacgga aaacgcgatc cagatcacaa atacattgta ttcacatcat taaccgtttt
     4861 aagatcattt catcactttt tcgcaactca cccgataatc tgttatgaca acaaacactg
     4921 tttcccgcaa agtggcgtgg ctacgggtcg ttacgctggc agtcgccgcc ttcatcttca
     4981 acaccaccga atttgtccct gttggcctgc tctctgacat tgcgcaaagt tttcacatgc
     5041 aaaccgctca ggtcggcatc atgttgacca tttacgcatg ggtagtagcg ctaatgtcat
     5101 tgccttttat gttaatgacc agtcaggttg aacggcgcaa attactgatc tgcctgtttg
     5161 tggtgtttat tgccagccac gtactgtcgt ttttatcgtg gagctttacc gttctggtga
     5221 tcagtcgcat tggtgtggct tttgcacatg cgattttctg gtcgattacg gcgtctctgg
     5281 cgatccgtat ggctccggcc gggaagcggg cacaggcctt gagtttaatt gccaccggta
     5341 cagcactggc gatggtctta ggtttacctc tcgggcgcat tgtggggcag tatttcggtt
     5401 ggcgaatgac cttcttcgcg attggtattg gggcgcttat cacccttttg tgcctgatta
     5461 agttacttcc cttactgccc agtgagcatt ccggttcatt gaaaagcctc ccgctattgt
     5521 tccgccgccc ggcattgatg agcatttatt tgttaactgt ggtggttgtc accgcccatt
     5581 acacggcata cagctatatc gagccttttg tgcaaaacat tgcgggattc agcgccaact
     5641 ttgccacggc attactgtta ttactcggtg gtgcgggcat tattggcagc gtgattttcg
     5701 gtaaactggg taatcagtat gcgtctgcgt tggtgagtac ggcgattgcg ctgttgctgg
     5761 tgtgtctggc actgctgcta cctgcggcga acagtgaaat acacctcggg gtgctgagta
     5821 ttttctgggg gatcgcgatg atgatcatcg ggcttggtat gcaggttaaa gtgctggcgc
     5881 tggcaccaga tgctaccgac gtcgcgatgg cgctattctc cggcatattt aatattggaa
     5941 tcggggcggg tgcgttggta ggtaatcagg tgagtttgca ctggtcaatg tcgatgattg
     6001 gttatgtggg cgcggtgcct gcttttgccg cgttaatatg gtcaatcatt atatttcgcc
     6061 gctggccagt gacactcgaa gaacagacgc aatagttgaa aggcccattc gggccttttt
     6121 taatggtacg ttttaatgat ttccaggatg ccgttaataa taaactgcac acccatacat
     6181 accagcagga atcccatcag acgggagatc gcttcaatgc cacccttgcc caccagccgc
     6241 ataattgcgc cggagctgcg taggcttccc cacaaaataa ccgccaccag gaaaaagatc
     6301 agcggcggcg caaccatcag tacccaatca gcgaaggttg aactctgacg cactgtggac
     6361 gccgagctaa taatcatcgc tatggttccc ggaccagcgg tacttggcat tgctagcggc
     6421 acaaaggcaa tattggcact gggttcatct tccagctctt ccgacttgct tttcgcctcc
     6481 ggtgaatcaa tcgctttctg ttgcggaaag agcatccgaa aaccgataaa cgcgacgatt
     6541 aagccgcctg caattcgcag accgggaatc gaaatgccaa atgtatccat caccagttgc
     6601 ccggcgtaat acgccaccat catgatggca aatacgtaca ccgaggccat caacgactga
     6661 cgattacgtt cggcactgtt catgttgcct gccaggccaa gaaatagcgc gacagttgtt
     6721 aatgggttag ctaacggcag caacaccacc agccccaggc caattgcttt aaacaaatct
     6781 aacattggtg gttgttatcc tgtgtatctg ggttatcagc gaaaagtata aggggtaaac
     6841 aaggataaag tgtcactctt tagctaacct tgcatcgcat tgaccaaaac ttgaaccgat
     6901 ttagcaaaac gtggcatcgg tcaattcatt catttgactt atacttgcct gggcaatatt
     6961 atcctctgca actaattact tgccagggca actaatgtga aaagtaccag cgatctgttc
     7021 aatgaaatta ttccattggg tcgcttaatc catatggtta atcagaagaa agatcgtctg
     7081 cttaacgagt atctgtctcc gctggatatt accgcggcac agtttaaggt gctctgctct
     7141 atccgctgcg cggcgtgtat tactccggtt gaactgaaaa aagtgttgtc ggtcgacctg
     7201 ggagcactga cccgtatgct ggatcgcctg gtctgtaaag gctgggttga aaggttgccg
     7261 aacccgaatg ataagcgcgg cgtactggta aaacttacca ccagcggcgc ggcaatatgc
     7321 gaacaatgcc atcaattagt tggccaggac ctgcatcaag aattaacaaa aaacctgacg
     7381 gcggacgaag ttgcaacact tgagcatttg cttaagaaag tcctgccgta aacaaaaaag
     7441 aggtatgacg atgtccagac gcaatactga cgctattacc attcatagca ttttggactg
     7501 gatcgaggac aacctggaat cgccactgtc actggagaaa gtgtcagagc gttcgggtta
     7561 ctccaaatgg cacctgcaac ggatgtttaa aaaagaaacc ggtcattcat taggccaata
     7621 catccgcagc cgtaagatga cggaaatcgc gcaaaagctg aaggaaagta acgagccgat
     7681 actctatctg gcagaacgat atggcttcga gtcgcaacaa actctgaccc gaaccttcaa
     7741 aaattacttt gatgttccgc cgcataaata ccggatgacc aatatgcagg gcgaatcgcg
     7801 ctttttacat ccattaaatc attacaatag ctagttgata acgtgacaac gtcactgagg
     7861 caatcatgaa accactttca tccgcaatag cagctgcgct tattctcttt tccgcgcagg
     7921 gcgttgcgga acaaaccacg cagccagttg ttacttcttg tgccaatgtc gtggttgttc
     7981 ccccatcgca ggaacaacca ccgtttgatt taaatcacat gggtactggc agtgataagt
     8041 cggatgcgct cggcgtgccc tattataatc aacacgctat gtagttattg tcaggctctg
     8101 atcacagagc ctgacatctc cgtaatatct tcctcaagtc tcaaatattt tcgccattgt
     8161 tggcgatgag atcccgatac cagtaaaacg acgctttacg tatccgttcc agcgttccct
     8221 tcccttcgtt gtctttatcg acataaatca ttccgtagcg ctttttcatt tcccctgttc
     8281 cggcagaaac caggtcaatg cagccccacg gggtgtagcc aattaagtca acaccatctt
     8341 caactacggc ttttttcatt tcccgaatat gggaagcaaa gtaatcaatg cgatagtgat
     8401 cgttcaccgt gccgtcagct tgtctctggt caaccgcacc aaatccattt tcgacaataa
     8461 acagcggcaa ctggaaatga tcccagaacc agttgagtga ataacgtagc cctgccggat
     8521 caatttgcca tccccattcg gatgtatcga tataagggtt gctgaccaaa tctcgtggtt
     8581 caacataatc cagttgcggg ttatcgggag aaaattgtgt cgtaaaagac atgtagtagc
     8641 taaaaccgat aaagtcgaca catccactgg caagaatcgc gttatcttct ggtgtgatat
     8701 cgaggttgaa tcctttcctg gcaaagtaat tcagcatatg ttgcggataa tatccacgag
     8761 catgaacatc agtaaaccag taacgacgat gcatcgcttt cgtggccatc atcatatcgt
     8821 tgggtgcaca cgtcagagga tagatggggc acatggcgat catacagcca atgttaaatt
     8881 caggattgat cgattttcca gtctgtaccg ccagggcact ggcaactaac tcgtaatgta
     8941 ccgcctggta cattatttgt tcgcgctcat tgatatcttc ctctggcgaa tacaagatac
     9001 cggaattagt aaatggacac aggctttcgc tgaaattcac ctgattattg atttcgttaa
     9061 acgtcatcca gtactttact ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga
     9121 taaaaaagtc gatcagctta cggtttcgcc agccaccata ttttgtcacc agatgataag
     9181 gcatctcaaa atgcgaaagc gtcaccacag gttccattcc ttgcttcagg cattcatcga
     9241 acagatcatc ataaaattgt aaaccctctt cattcggctc ctgttcgtca ccttgcggaa
     9301 agattcgtgt ccaggcaatg gaagttcgaa agcatttgaa tcccatctcg gcaaataact
     9361 gaatatctgt tttatagcga tgataaaaat caattgcttc atgattggga taattaagcc
     9421 cgtcgataac gccttctgtc acttcacgcg gcaccccgtg agcgccagca gtcatcacat
     9481 cagcgatact gatgcctttt cctccttcat tccagccacc ttccaactga tgcgcggcta
     9541 ccgcgccacc ccataaaaaa ccttttttaa atcctgacat acacaactcc ttaactaaaa
     9601 tagattacca ccagacttc
//
LOCUS       DAZSBF010000155         9542 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZSBF010000155 DAZSBF010000000
VERSION     DAZSBF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.158"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1321
                     /locus_tag="ACHXWD_003025"
     CDS             <1..1321
                     /locus_tag="ACHXWD_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR7621877.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHXWD_003026"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="ACHXWD_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR7621878.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHXWD_003027"
     CDS             2703..3482
                     /gene="ygfM"
                     /locus_tag="ACHXWD_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR7621879.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="ACHXWD_003028"
     CDS             3479..6418
                     /locus_tag="ACHXWD_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR7621880.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="ACHXWD_003029"
     CDS             6351..6665
                     /locus_tag="ACHXWD_003029"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR7621881.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHXWD_003030"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="ACHXWD_003030"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="ACHXWD_003031"
     CDS             complement(7120..7608)
                     /locus_tag="ACHXWD_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR7621882.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHXWD_003032"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="ACHXWD_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR7621883.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAZSBF010000156         9451 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZSBF010000156 DAZSBF010000000
VERSION     DAZSBF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.159"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(37..684)
                     /locus_tag="ACHXWD_003033"
     CDS             complement(37..684)
                     /locus_tag="ACHXWD_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TraJ family protein"
                     /protein_id="HFR7621884.1"
                     /translation="MCAMDTRERALIRQSGLPEIILSAKYPVCVRTESGKFIDSNSVF
                     LHLIKCQNSDSEIWFSGIDIDTQILFHTAEVDAFSSSNGVSIINGVILNDVSWDVVVE
                     STEVNNLNLFVWRFFERYVVSGFFIKGKEFGCEPDKYRFNKIIPYLLGYSHEYSSQRM
                     DISVDQSKKIFMNFKKHYGFSDRDEWLRYAIISDVLFYLYQYFASNINSLHCKEM"
     gene            complement(876..1259)
                     /gene="traM"
                     /locus_tag="ACHXWD_003034"
     CDS             complement(876..1259)
                     /gene="traM"
                     /locus_tag="ACHXWD_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFR7621885.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSAEMERFFPKNDDE"
     gene            1592..2182
                     /locus_tag="ACHXWD_003035"
     CDS             1592..2182
                     /locus_tag="ACHXWD_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFR7621886.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(2479..3300)
                     /locus_tag="ACHXWD_003036"
     CDS             complement(2479..3300)
                     /locus_tag="ACHXWD_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFR7621887.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(3411..3707)
                     /locus_tag="ACHXWD_003037"
     CDS             complement(3411..3707)
                     /locus_tag="ACHXWD_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621888.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            4007..4303
                     /locus_tag="ACHXWD_003038"
                     /pseudo
     CDS             4007..4303
                     /locus_tag="ACHXWD_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4622..4747)
                     /locus_tag="ACHXWD_003039"
     CDS             complement(4622..4747)
                     /locus_tag="ACHXWD_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFR7621889.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(4689..4838)
                     /gene="mok"
                     /locus_tag="ACHXWD_003040"
                     /pseudo
     CDS             complement(4689..4838)
                     /gene="mok"
                     /locus_tag="ACHXWD_003040"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(5017..5779)
                     /locus_tag="ACHXWD_003041"
                     /pseudo
     CDS             complement(5017..5779)
                     /locus_tag="ACHXWD_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(5776..6210)
                     /gene="psiB"
                     /locus_tag="ACHXWD_003042"
     CDS             complement(5776..6210)
                     /gene="psiB"
                     /locus_tag="ACHXWD_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFR7621890.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(6265..8223)
                     /locus_tag="ACHXWD_003043"
     CDS             complement(6265..8223)
                     /locus_tag="ACHXWD_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFR7621891.1"
                     /translation="MSVTESKTKTERKSSRKPAKTQETVLSALLEQTEEVSVSLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGILPADWPVRVKVIPQELATAASMTENGQRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGGGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLTLLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDTLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            complement(8287..8520)
                     /locus_tag="ACHXWD_003044"
     CDS             complement(8287..8520)
                     /locus_tag="ACHXWD_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFR7621892.1"
                     /translation="MSEYFRIFQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNSGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            complement(8576..9097)
                     /locus_tag="ACHXWD_003045"
     CDS             complement(8576..9097)
                     /locus_tag="ACHXWD_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFR7621893.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDKGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPQPQQNGQPQSADTTKKGSAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
BASE COUNT         2274 a         2592 c         2235 g         2350 t
ORIGIN      
        1 gttaacaaaa accggcactt ttagtgccgg gaatgcttac atttctttac agtgaaggct
       61 gttaatatta ctagcgaagt attgatatag ataaaataac acatcagaga ttatagcata
      121 tcgcaaccac tcatccctgt ctgagaaacc atagtgtttt ttaaaattca taaatatttt
      181 tttagattgg tcaacagaga tatccattct ctgagaagaa tactcatgac tataacccaa
      241 aagatacggt attatcttat taaagcgata tttgtcaggt tcacaaccaa actccttgcc
      301 ctttataaaa aagccactaa ctacgtatct ttcaaagaat ctccagacaa acaaattcaa
      361 gttattcact tccgtacttt caaccaccac atcccatgat acatcattca atattacacc
      421 attaataata ctcactccat tcgaggacga gaaagcatca acttcagcag tatgaaataa
      481 aatttgcgta tcaatatcaa taccagaaaa ccatatttca gaatcactat tctgacactt
      541 tattaagtgt aaaaaaacag agtttgagtc tatgaatttt cctgactctg tgcggacaca
      601 gactggatat tttgcggata aaataatttc cggcaggcct gattgacgta tgagtgctct
      661 ttctctagta tccatcgcac acataggaac ctcctcaaag gattctatgg acagtcgatg
      721 cagggaggga caagctccct gcatcggcga ttttcaagtt caaattttaa cgtggcatta
      781 accacgtaat taatatacta attattctga ttattgtcat cagtttgaac cattaaaaag
      841 tctgatattt gaacaaagtc aaaatttatt tccctttatt catcatcatt ttttggaaag
      901 aacctttcca tctcagccga caccttctct ctgatatctt caaccatatt ggcatattca
      961 aactttggat ttcctgagac atgaggactg agagactcaa tacccaaaat tttcgctact
     1021 gatgactgtg tttttacaac gcattcaaga agtagtttat taaactcggc ctgattaaat
     1081 gcagactctt tgcgctccat ctgagcctca tatacacgaa gacccagttc aagaagcatt
     1141 gaagccgttg ctgaaaaact gacatctttt tctctcgccc cctcctgtcg acgtttttca
     1201 ataatcgcgt ttattttttc ataagcatca ttgctgatat acagattcac cttagccatt
     1261 agatattacc ttttttaaac ataaaacaaa gttaatttcg aaacactaga attcatagtg
     1321 caaacaggga cgcaccgcta gcagcgcccc tgacggtatc ctataaaaaa acacaccgcg
     1381 ccgctagcag cacccctaat ataaaataat gttttttata aaaatagtca gtaccacccc
     1441 tacaaagcgg tgtcggcgcg ttgctgtagc tgcgttaacg acgctgcttt aaaaaaatca
     1501 gatttaaaca atataaatcc acaaatacaa ctcaatgata ttaaagataa atcagcaaaa
     1561 acttgttttt gcgtagtgtg tggtgctttt ggtggtgaga accaccaacc tgttgagcct
     1621 ttttgtggag tgggttaaat tatttacgga taaagtcacc agaggtggaa aaatgaaaaa
     1681 atggatgtta gcaatctgcc tgatgtttat aaatgggatc tgcgaagccg ccgattgctt
     1741 tgatcttgca ggtcgggatt acaaaataga cccggattta ctgagagcga tatcatggaa
     1801 agaatcccgt taccgggtta atgccatcgg tattaatccg gtaacgggat atggcagcgg
     1861 actgatgcag gtagattccc agcattttaa cgaactggcc cgctatggaa ttaagccgga
     1921 acatctgaca acagatccct gtatgaacat ttataccggt gcttattatc tggcaatagc
     1981 ctttaaaaaa tggggcgtct cctgggaggc cgttggtgca tacaatgccg gattcaggaa
     2041 gaccgaacgc cagaaccaga gacgtcttgc ctacgcatca gaggtttacc ggatttatac
     2101 ctggataaag agcagtaaag gcatccgggt tccgaccacg aagaaatcac tttcccaaat
     2161 taacagtgtg cagaaaaatt aatattgtat acattgtcaa ataccggtta tgccgcgaca
     2221 gcggcatgga aggccacaca caaaaatatc atatgagtga taatgctgac tggccgaagc
     2281 cccgcaaagc gggatcccat cttatatata tccggtaact gcgcagcctc ctgccagacc
     2341 atttttcgcg ggcacaatat ttacagacta ccgaaaagcc agttgatgaa attgaggcat
     2401 atcagacgac acagcaatgc cgcaggatga taatataagt aaagccccga aaaatttttc
     2461 ggggctttac ttatattatc atgacttcca ctccctgaac tgttcggcaa tcatccataa
     2521 tgctttgttg attcggatat caccgtcaat gcctgtaatg gctcttgttc tggtattttt
     2581 accggaggca cttcgccccg ataatccacc ttttatcata ttctcctgaa cccgctgcca
     2641 ggttgtccag agatcgttct gtttatcctc ccaacggcgg ggagtaatta tttgttctgg
     2701 cgtcactggc gttttatttt catcttcgta cctggccatc aatgcagctc tgccaaataa
     2761 atgttgttcg tcactgttaa gatgtatctc tttcattgtc tccatatttt cagtgacttt
     2821 atcaaagaca ccgagcactt catacgctcc ctcaataacc tgcccgacaa tatcaccttt
     2881 atgtggaacg cggatttcgc caaaattatt cccacacacc aggccatttg tgcagacaaa
     2941 acgaaaaatt cctgggatca tctgataact ggatgaacca tcatgtgaat taagcaggat
     3001 aatttcagga acttctttcc cgttaatatg cccttccctg cgaagacgta acatatgttt
     3061 actgtattcg cggcgtccca aatcacgaac ccgactctga caggcaaaga atggctggaa
     3121 accttcatca cgtaacttat tgatgatatt gatggttggg atatatgtat aacgctcact
     3181 ccgggattca tgtttatcac cagaaaagac agatggaaca aactgcatta attcgtcatc
     3241 cgttaacggg cgctcacggc gaatagaatt atatcgacca aaacgggaag ctaatcgcat
     3301 aacagtaact cctttatgaa ggcataaaat taataaaagt tttttcgctt aatatcatcc
     3361 cccctcggtt atcgttaaca aaaagacaac aattccgagg ggaaggacca tcaaatcagg
     3421 tcagttcggg actgaccaac ctgccgagtg aaaagcagta acagcgcgat caactcatca
     3481 gcgttttttc tgacaagagg agtatgattc tccaggctat ccagtaagtc atgcagctca
     3541 ttattgacct ggtggagcac cttaaagagt gcaccagaat cccggcagct ctgatatgca
     3601 agatttgcct gaacagcacg attctgcatg atcccgattt gtgcgattgc gtctttctgc
     3661 tgatcgcctg ttggctgata agtggtgtta aaagaaaatg cgctcataat tcctcctgta
     3721 tctgatgttt tcagccccca tcatctgttg agtttcggga gccgcggttt tgacgttttg
     3781 cggggacctg ccttttgttc cctccggtat tcaccatcac ctgtttgaga accttcgccg
     3841 caaggggcat cactgccacg gcctgacgca cctgtcctgg gcagaacgca aaatttttta
     3901 ccgggttttg ggtaaaaaat tttgtgggct gttcatttac cctggacagt cagtgcggca
     3961 ggttgtggct gtctatgccc ccggcgacag gggcttaaac aggtgaatgg caccggacag
     4021 aacaacgggc agggacccgc cgcaacgacg tgaagcgcgg ttcgcgaaac ggcgttgctc
     4081 cttacatccc ggcgtctttt gccgggatgc cgggagcgca ggccacggcg cgacaagggt
     4141 ggcgcgaccg ttccggtcgc tgcaaacccg ggtttttgcg tcagggatgg aagcccgcca
     4201 gggcggagac agcttcgctg gctccgttca cgacagcccg ccccgccagg gggacgcccg
     4261 gaaaaggcat aagcaggaga acaacaaaac agacaataat cagggcagca gtaatcagag
     4321 tcagagcagt aatcagagtc agagcaggag gaaaaagaga ataatcaaag ggaaggtgat
     4381 gtgatttctg gtaagtcata aacagagaag gcgggggaaa agaacgggcg ccgcaggcgc
     4441 acgttcatcc ccgttcccgg agggaaacgg ccttcctggc agtctggttg ttcatgcccg
     4501 tacaatgcca gcaaggagaa aggggccacc ggcgaaccag cagccccttt ataaaggcgc
     4561 ttcagtgcag tcagaccagc atcagccctg aaaaggcggg cctgcgcccg cctccaggtt
     4621 gctacttacc ggattcgtaa gccatgaaag ccgccacctc cctgtgtccg tctctgtaac
     4681 gaatctcgca cagcgatttt cgtgtcagat aagtgaatat caacagtgtg agacacacga
     4741 tcaacacaca ccagacaagg gaacttcgtg gtagtttcat ggccttcttc tccttgcgca
     4801 aagcgcggta agaggctatc ctgatgtgga ctagacatag ggatgcctcg tggtggttaa
     4861 tgaaaattaa cttactacgg ggctatcttc tttctgccac acaacacggt aacaaaccac
     4921 cttcacgtca tgaggcagaa agcctcaagc gccgggcaca tcatagccca tatccctgca
     4981 cgctgaccac actcactttt cctgaaaata acccgctcat tcaggctgtt tacgggtaat
     5041 ctgtgtgatt gttgccgcat cacgccgcct cccggagttt gttcggcacc atccaccgct
     5101 ccatctcacg gacagcgtga ccggcctctc ttgctgcaag actggcttcc gcaataacct
     5161 gccagaacgg catatcctgc cactgccagc gttccattcc ggccagtgac ggaaaacgct
     5221 caccccactg ccagaacagg gtttccaggt catgctctgc cacccggtca ctccagcgtg
     5281 cataccagct tccgactgtt tccggcgaat ggaatgccag ttcgatttca gcctgagcga
     5341 ccagcgcctg gtacgcacgg gctttctgct ctgcctccct cgcctgctgg cggctgtact
     5401 tctgcatgac cagcgcggat ttatggcgac tgcgttccct gacacgaaac cgcaccgacg
     5461 ggaacaagcg ttctgctgca tcgcctggaa gaggaaggag gcagacctcg ccctgtgttt
     5521 caatcagcac atcgatggca tacagccact gaagacggct gccgtgcagt tcttctgcgg
     5581 tcagggacgg catgaaaaat cgcaggtcag acagcgatat cctgcggctt ccgttcagac
     5641 agcggaaaaa cgcccccgca tacggatatt ccgccagcgt gtttccctgg tggcggcgtt
     5701 tttctgtctc cgccaccgcc cgccatgcgg cctgttgctc tgcgctgagg ggaaccagtg
     5761 cctgtgaacg tgcgctcatg cctgacctcc ttccgccatc aggatatgga tgatgtcact
     5821 gacgctgtac ccctgcgcgt ccagtgacgc cgccagtgcc agtgaatggc tgacggcttc
     5881 cggtgcaaac cgccgctgta cctgaaccac cgcaaacggc tcaccaccgg cattcaccag
     5941 aaccagcacc cagacctgag agacatcgcc cggtgaacat aacgccaggt gaaaacgttc
     6001 gtgggcaggc gtgaaacgga cctggacggg gaaaaatccg ccaaactcgc tgccgtactc
     6061 gccgttcatc gtccagttgt ccggcgcatc cagttcccgc atcacggcgt gtgtcagctc
     6121 acgacgttca tcacttccgg cagcccggat atcttcatat tcctgtgcgc tcatggtctg
     6181 taatacattc agggtcagtt cagttttcat cacggtatct cccttgctgc cgccccgtct
     6241 ccggcggggc ggtgtggttg ttcatcaggc ggcatcagcc aggttatcgg tgcgttcagt
     6301 ggtatctgtc tgtggatgtg gtgcacacat ccagccaggc acccagcggt tgtctttcat
     6361 atggtgctct gcatgttcag ccgcatcacc tttcttcatc ttctccgcgt cccgtgcggc
     6421 accggacagc cctgcctcat tcagggctgc gataatctgc ggctttttca ggtgtccgaa
     6481 gaagtttgct tttgtcggct gccaccagtc gcgcatgtga aagccgatgg cgctttccag
     6541 cgtgtcaagc ggactgcgtg acgtgtgacc acactcacgg gtctggacac cgttaaggct
     6601 gcatgcggtg cagaaactga gcagggataa cagcacctcc tgactgagtg acaggaatgt
     6661 cgtcatgtcc cgtgaccatc cctccggtaa cagggcagca agacgggctt tttctgccat
     6721 cagcgccatg aatgcggcac cttccttccc cgatggcgca tcgctggtca gcgaataatg
     6781 tttacattcc aggctgattt gtgctggttt actgtacgct ccgctgccaa acacattcag
     6841 gcagagcgtc catgccagca gtgtcaggga tttgtccggt tgctgcatca gtgctgcctg
     6901 gactgccagc gtgcgttcgg aagacatttt cgtcagcagt ggcagactga tttcctccac
     6961 tgacgctttc tcctgcacct gttccgtacg gtcagcgtcg tcagccacgt catcctcact
     7021 gcgcaactgc acaccacgct ggacacacac gttgccataa cgccaggaca ccaccactcc
     7081 acctccggca cgcatctccg gcgtccacgc cctgaccttc gccatgcagc gcatcagctt
     7141 catttctgct tccagcaggt cagattcctc acactggttt tccagcgcgt cgtaacgcgt
     7201 catcagttcg ttcagacgtt cttcttccgc ctccgtcagc accgcttccg gctccggcag
     7261 acagcggtat gttccggcat cctcacggca ctcaccgaca ggctccatgc gtccggcgca
     7321 ccattcccag ccttcggctt cccgaaggaa ctctgcgaca gcctggagtt tttccagcag
     7381 ggcggcatcg agcgccacgc agtccacata gccaccttcg tcgtcgctga acaaatcggt
     7441 gcgcagttcg tctggcgaga aggcatcagc ccccacgaag cggaatttac tgttccccgc
     7501 caccgccact tcactttcgg tcaccagacg acgaatggtc tgtacttccg gtttaccgcc
     7561 ccatcccgac tgacaggcgg cttcaaacac ctgcacctga cgcgcggtgt cgttctccag
     7621 cgccagcgcc tgacagtgtt cggtggtgat gcggtcttct gccagcgcat cgaggatgac
     7681 aggcgcaagg tcagccagtt tcagcattcg ctgaacgtgg cggggtgaat agcccagcaa
     7741 atcaccgatt tgtgcaggtg ttttgccttc ctgcgccatt gcgcggaatc cggcaatctg
     7801 ttcggcaggg tgcatatccc gacgctgacc gttttcagtc attgatgcag ccgtcgccag
     7861 ctcctgcgga ataaccttca cgcgtacagg ccagtcagcc ggaaggatgc cacgctctgc
     7921 cagcatgttg agtgctgcca gtcggcgacc acctgcggcg acaccgtggc ggtcaccagg
     7981 cagggcatga acaaccagat tctgcaacag gccaacgccc ttaatggact ccgccagttc
     8041 gctgacggac tctgcggaat acggcaccgt gcgcacattc agcggtgatt taatcagcga
     8101 ggccagcgac acgctcactt cctccgtctg ctccagcagg gcagacagga ctgtttcctg
     8161 cgtttttgca ggtttacggc tggatttacg ctccgttttt gtcttagact ctgtaactga
     8221 catatatttc tcctttgacc gggatggttg tatgtctgcc ggacgcgttc ccgtccggca
     8281 cctctcttac ttaagaatgc cgaaattccg gatgacatgg ttcatccagt acccggcacc
     8341 gtcctcaaaa ttccaggtac gccagaccag gccactgtta ttgcggacaa ccaggcgaaa
     8401 atgcgttccc tgatcatcct cgataaagac attccggtac tgtgcggcaa cggcttcagc
     8461 ctgttcgcgg gtaaaggagc cgtcaggcag cccctgaaat attctgaaat attcgctcat
     8521 cctcatatct ccggtgatgc cccgcactgg acggggcggt tgtcactgtc tttcgttaga
     8581 acggaatgtc gtcatcgaag tcatacgcgg gttcagacgg ctgctgaggc tccggctgcg
     8641 cggccttacg tccacggcct tttgttttcg cgctcccttt tttcgtcgtg tcagcactct
     8701 gtggctgacc attctgctga ggctgcggtt gcgcctgagc gttctgctgt ggtgcactcc
     8761 ccagcatctg catggtgccc gtggttttca caaggatttc agtgacgtaa cgggtaatac
     8821 ctttatcttc ccagctacgg gttcggagtt gcccctcgat gtagacctgc gcgcccttgc
     8881 gcagatattc acctgccact tccgcgagct tgccgaacag caccacgcga tgccattctg
     8941 tctgctcccg catctccccc gtctgtttgt cacgccagct ttctgacgtg gccacctgca
     9001 ggtttgccac tgcgcccccg ttggggatgt aacggacttc cggatctttt cccagacgac
     9061 cgacaagaat gaccttgtta atgccacgaa ctgccatgat gtgtctcctt ctgttgatgt
     9121 tttcagcccc catcatctgt tgagtttcgg gagccgcggt tttgacgttt tgcggggacc
     9181 tgctctttgt tccctccggt attcaccatc acctgtttga gaaccttcgc cgcatggggc
     9241 atcactgcca cggcctgacg cacctgtcct gggcagaacg caaaattttt taccgggttt
     9301 tgggtaaaaa attttgtggg ctgttcattt accctggaca ggtgcggcag gttgtggctg
     9361 tctatgcccc cggcgacagg ggcttaaaca ggtgaatggc accggacaga acaacgggca
     9421 gggacccgcc gcaacgacgt gaagcgcggt t
//
LOCUS       DAZSBF010000157         9432 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZSBF010000157 DAZSBF010000000
VERSION     DAZSBF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.160"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHXWD_003046"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHXWD_003046"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR7621894.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHXWD_003047"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHXWD_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR7621895.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHXWD_003048"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="ACHXWD_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHXWD_003049"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHXWD_003049"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR7621896.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHXWD_003050"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHXWD_003050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR7621897.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHXWD_003051"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHXWD_003051"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR7621898.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHXWD_003052"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHXWD_003052"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR7621899.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHXWD_003053"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHXWD_003053"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR7621900.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHXWD_003054"
     CDS             complement(6556..7449)
                     /locus_tag="ACHXWD_003054"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR7621901.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHXWD_003055"
     CDS             complement(7446..8099)
                     /locus_tag="ACHXWD_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR7621902.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHXWD_003056"
     CDS             complement(8110..9369)
                     /locus_tag="ACHXWD_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR7621903.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAZSBF010000158         9397 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZSBF010000158 DAZSBF010000000
VERSION     DAZSBF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.161"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHXWD_003057"
     CDS             197..301
                     /locus_tag="ACHXWD_003057"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR7621904.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHXWD_003058"
     CDS             complement(350..1603)
                     /locus_tag="ACHXWD_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7621905.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHXWD_003059"
     CDS             1710..2603
                     /locus_tag="ACHXWD_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7621906.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHXWD_003060"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHXWD_003060"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR7621907.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHXWD_003061"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHXWD_003061"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR7621908.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHXWD_003062"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHXWD_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR7621909.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHXWD_003063"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHXWD_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR7621910.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHXWD_003064"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHXWD_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR7621911.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHXWD_003065"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHXWD_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR7621912.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="ACHXWD_003066"
     CDS             complement(8323..>9397)
                     /locus_tag="ACHXWD_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR7621913.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2438 g         2131 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAZSBF010000159         9217 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZSBF010000159 DAZSBF010000000
VERSION     DAZSBF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.162"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            73..732
                     /locus_tag="ACHXWD_003067"
     CDS             73..732
                     /locus_tag="ACHXWD_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR7621914.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="ACHXWD_003068"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="ACHXWD_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR7621915.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="ACHXWD_003069"
     CDS             complement(1860..2570)
                     /locus_tag="ACHXWD_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR7621916.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="ACHXWD_003070"
     CDS             3004..3294
                     /locus_tag="ACHXWD_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR7621917.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="ACHXWD_003071"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="ACHXWD_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR7621918.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            3927..3998
                     /locus_tag="ACHXWD_003072"
     CDS             3927..3998
                     /locus_tag="ACHXWD_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621919.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHXWD_003073"
     CDS             complement(3975..4127)
                     /gene="hokA"
                     /locus_tag="ACHXWD_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR7621920.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHXWD_003074"
     CDS             complement(4453..6522)
                     /gene="glyS"
                     /locus_tag="ACHXWD_003074"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR7621921.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHXWD_003075"
     CDS             complement(6532..7443)
                     /gene="glyQ"
                     /locus_tag="ACHXWD_003075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR7621922.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(7538..7837)
                     /locus_tag="ACHXWD_003076"
     CDS             complement(7538..7837)
                     /locus_tag="ACHXWD_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR7621923.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            8012..9007
                     /gene="wecH"
                     /locus_tag="ACHXWD_003077"
     CDS             8012..9007
                     /gene="wecH"
                     /locus_tag="ACHXWD_003077"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR7621924.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(9049..>9217)
                     /locus_tag="ACHXWD_003078"
     CDS             complement(9049..>9217)
                     /locus_tag="ACHXWD_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR7621925.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         2287 a         2331 c         2268 g         2331 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagctgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gcacagaatc taagatccct gccatttggc ggggattttt
     3841 ttatttgttt ttcaggaaat aaataatcga tcgcgtaata aaatttagta ttatttttgt
     3901 gaagaataaa tttgggtgca atgagaatgc gcaacgccgt aagaaaggcg ggaataactt
     3961 cccgccaaag actcttactc tttcaaattg caggctaaaa atgccgccag ctcataactc
     4021 tcctgtttaa tatgcaattc acacagtgaa tctcttatca tccaggtgaa aaataaaagc
     4081 gtgaaacaaa tcactattaa agaaagtaat ctatatttct gcggcattcc cagcctcctg
     4141 tgttgatttc caacgagtaa gaggctaacc ttatggtgtt caggcatagg gtagcctcac
     4201 tttgatttat agtcaggtgg ggcttttctc tgtctgcctt tcggtgaata cctgagacaa
     4261 acagtctcaa gcacccgtgg ctattctagc ttaataagtt tgtttcttct ccttgatata
     4321 atcctaaaaa aacctcataa aattaatata tgagataatc tttattcaac agaagattat
     4381 taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc aggcttttta
     4441 ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca gttcgcgcag
     4501 tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca tgaccatcac
     4561 tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca gcgcatcctg
     4621 gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa cctgcatcgc
     4681 cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc tcagcacttc
     4741 gtcagatttc gccagaatgt tagatacacg tttgttcgcc gccgccagtg cagcagctgc
     4801 atccagggta cggaaatgcg ataccgcttt catacgggca tcgaaatcag ccggacgagt
     4861 cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt cctgatacca
     4921 ggcgcggaag cgaccgagca taaagtcgat aacatcatca actacgttgg cgttagtcag
     4981 cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt
     5041 cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt
     5101 gtcgcctttc ggatgctgac cgataccgaa gatacccgcc agagtatcca tcttatcagc
     5161 aatcgccagc gcacaagcta ccgggttgga cggcaggtca tcaccggcaa agcgcggctg
     5221 atactgctca ttcagcgcga cggctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg
     5281 catccccata acgccttggg tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca
     5341 cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct gttcagcaat
     5401 ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca gctgttgctg
     5461 gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac ggtcggtatt
     5521 gaagaagaac tcggcatccg ccagacgtgg acgaacgact ttctcgttac cagagataat
     5581 ttgctgcgga tctttcgatt cgatgttggc aacgaagatg aagttcggca gcagtttgcc
     5641 atcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa ccagggcttc
     5701 agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg gccactccac
     5761 cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc caatcttacg
     5821 cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat cggcgatgac
     5881 tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg tgaattccgg
     5941 ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca gaatggttgc
     6001 cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca cgaagtgcac
     6061 gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagcg aagtcgcaac
     6121 catattcggc agtagtgctt cagtgctttc gcccttcaca tgggcgcgat acagcagcca
     6181 ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc
     6241 ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg caatcgccgg
     6301 gccgcgtttt tcgatttcac gatctggttg cgcttccgcc aggttagcta ctttcaacgc
     6361 cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag cgttatccag
     6421 ctccgcagta aagttcgcag caaaggactc agccaggctg cgcagtgctt ttggtggcag
     6481 ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct cttacttatc
     6541 tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt ctgccactgc
     6601 tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcagtgacgg agatggcttt
     6661 acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt aggctggcag
     6721 cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact gctcgaagca
     6781 ggtgaacagg aaatccacat ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt
     6841 ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc agaccaggtc
     6901 gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt aggtgatctc
     6961 gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga actgcgtcac
     7021 ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag tcgggttttc
     7081 ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca gctctttcag
     7141 agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca cctggaactg
     7201 atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg ggcgacgaga
     7261 aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac aggtcattgg
     7321 gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc agccctggcg
     7381 agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat caaacttttg
     7441 catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat acccgctgga
     7501 tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa agatgcaaaa
     7561 aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta taaccgcgca
     7621 tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt
     7681 tataacgttt tgccgcctga tccttgcata acgcttccat attcaacgaa cgcagaggat
     7741 caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaccaaccc actagcacca
     7801 taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct ggtttgcagg
     7861 tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc ttaagcctct
     7921 ataagctgta gtgataaaac tcagattatt ccagtgcgcc aaccaaatga tttatcggtc
     7981 agactttttt taaccagata cagagacaca gatgcagccc aaaatttact ggattgataa
     8041 cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct ggtatgtgac
     8101 caatgctcat agtgttagcc ccgtcacatg ggatatcgcc aatgttctga attctgcctc
     8161 tcgtgtcagc gtgccgctat ttttcatgat ttccggctat ctcttttttg gcgaacgcag
     8221 cgcccagccg cgccatttct tgcgtatcgg cttatgtctg attttttata gcgcaatcgc
     8281 actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga aaaacctgct
     8341 gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga tttatctggt
     8401 ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc taatggcggt
     8461 gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt ttgaatggct
     8521 gccaattaac ttatatatca atggcgatac tttttactac attctgtatg gcatgttggg
     8581 ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga gcgccgcgct
     8641 gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc agtggcgcgg
     8701 aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct gcgcaatcgc
     8761 gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac ttggcttaat
     8821 ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg cgctgcgcac
     8881 ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct tttgcgcgac
     8941 gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa acagattagt
     9001 gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt attcgctgta
     9061 ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac gaatattctt
     9121 ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa aacctttttc
     9181 actgagtaat aatgtcgcat tccacagacc taccatc
//
LOCUS       DAZSBF010000160         9206 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZSBF010000160 DAZSBF010000000
VERSION     DAZSBF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.163"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..84)
                     /locus_tag="ACHXWD_003079"
     CDS             complement(<1..84)
                     /locus_tag="ACHXWD_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7621926.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
     gene            complement(487..1587)
                     /gene="chaA"
                     /locus_tag="ACHXWD_003080"
     CDS             complement(487..1587)
                     /gene="chaA"
                     /locus_tag="ACHXWD_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR7621927.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1857..2087
                     /gene="chaB"
                     /locus_tag="ACHXWD_003081"
     CDS             1857..2087
                     /gene="chaB"
                     /locus_tag="ACHXWD_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR7621928.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2245..2940
                     /gene="chaC"
                     /locus_tag="ACHXWD_003082"
     CDS             2245..2940
                     /gene="chaC"
                     /locus_tag="ACHXWD_003082"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR7621929.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2984..3337)
                     /gene="ychN"
                     /locus_tag="ACHXWD_003083"
     CDS             complement(2984..3337)
                     /gene="ychN"
                     /locus_tag="ACHXWD_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR7621930.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3522..4916
                     /gene="ychO"
                     /locus_tag="ACHXWD_003084"
                     /gene_synonym="iatA"
     CDS             3522..4916
                     /gene="ychO"
                     /locus_tag="ACHXWD_003084"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR7621931.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4917..5567)
                     /gene="narL"
                     /locus_tag="ACHXWD_003085"
     CDS             complement(4917..5567)
                     /gene="narL"
                     /locus_tag="ACHXWD_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR7621932.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5560..7356)
                     /gene="narX"
                     /locus_tag="ACHXWD_003086"
     CDS             complement(5560..7356)
                     /gene="narX"
                     /locus_tag="ACHXWD_003086"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR7621933.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7695..9086
                     /gene="narK"
                     /locus_tag="ACHXWD_003087"
     CDS             7695..9086
                     /gene="narK"
                     /locus_tag="ACHXWD_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR7621934.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2244 a         2240 c         2375 g         2347 t
ORIGIN      
        1 gacaatcgct gcggtaataa tccccgccag gatcggtgct gccaggtcgt gccagaaagt
       61 catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca aggcggctat
      121 tcttgaagtg tctggtttca agattagccc ccgttttgtt gtcaggtttt acctctcaac
      181 gtgcgggggt tttctctttc cagcaaccaa tgccaccagg gataaagccc ccgcaacatt
      241 gcgcctcacc ggataattcc ggcttggtgt ggatactacg tctcaattca tcttcacttc
      301 atccctgaaa tgtttgcaat aaagagtaca ttccggcttt tcaacagctg ttgcagtggt
      361 ttcgtgcagg ttgtggtaca ggctcgcaat tctgattaac gagctgtcca ggtagtatga
      421 agaaatacaa taagcaggaa tttatcttat ctcccccgat ttccgtcatc ggaggagata
      481 taactgtcag gcaaatatcg tcatcaaata ggcggcaaac agtgccagat gcgctgcgcc
      541 attgagcacg ttagtacgtc cggtggagaa ggagatatgg cacagcacta aagaggccac
      601 catcaccacc atttctggcg caccaagtgc aaactgtaat tcgttacccg tcataaaggc
      661 aattagcgtg acgacaggta cggtaagcga aatggttgct aacactgaac caaagaacag
      721 attcattgcg cgctggacct ggttgttcaa cactgctttt aacgcaccta aaccttccgg
      781 cgacagaatc aacagagcca ccaggaagcc agtaaaggcg acaggggcat tcatgctgtc
      841 gagcaatgtc tccagcgggc tggcattcat tttggtgact gcaataacgg caatcagatg
      901 gataatcaac cagatagcat gccacacgct gctatgggca gacggtttac cgtggtgcgg
      961 gtcgtcgtca tcactgtcat cttcgtgctc gtagacaaac aaactttgat gcgttttggt
     1021 ctggatcagc aaaaatacgc catacattgc cgcagaaatt aatgctacca gtaacgcctg
     1081 accggttgaa aaattcgccg caggcagagc cattggaaat accagtacga ttatcgccag
     1141 ggggaacagc gcaattaaat actgcttgat accaaacaga ttcatatatt gggtggcaaa
     1201 cttacgaccg cccaacaata atgaaaagcc aaccagccca ccggtaacaa tcataatgat
     1261 tgaatagagc gtatcacgca ttagcgttgg cgcggcgtcg ccggttgcca ttaaagctga
     1321 aatcaaactg acttcaagaa taaccactga aaggctaaga ataagcgaac cgtaaggttc
     1381 tcccaggcga tgggctaata cgtccgcatg acggacaaca ctaaaggcgc tacttaaaat
     1441 accaataagc gcaagaagat tgatggcaat gaccactggt agtgtctggc tgcttcccca
     1501 caggaacagc actaccagcg ccagaaccgg gaaaataagc gaagtctcct tgtggcgggt
     1561 ttttaccgcc tcttgagcat ttgacattat ggttatccct ttgcagatga atttatcgaa
     1621 aatgtaaaaa ataggtagga aaaataacag aaagtgtctg gatatcggta acattttacg
     1681 aatttttacc ccgctgtcga ttttttactc attggggcat aaaaataagt attacgttta
     1741 gacaatgttt gttttagcgt ttcttaagaa cagtctgaca tgaaaattct tatgttttgg
     1801 caagagtaga tattgttgac cacacttaat gttcaacttt gtaaaaggag tcaacgatgc
     1861 cgtataaaac gaaaagcgat ctgccggaaa gcgtaaagca cgttctaccg tctcatgccc
     1921 aggatatcta taaagaagcg ttcaacagcg catgggatca atataaagat aaagaagatc
     1981 ggcgtgatga cgccagtcgc gaagaaaccg cgcataaagt ggcctgggct gctgtgaagc
     2041 atgaatatgc caaaggggat gatgataaat ggcataaaaa atcgtaaaac cggtagctta
     2101 gttaaagcta ttcgtgcggt gttgccttgc aagtggtccg tggattgcat attgtcccgt
     2161 tagtggtttc aaaatgagcg gtaaaaatgt ccggaagaca ccaaaaagtt gtcgcaggga
     2221 agtatgcagt ggcggaggtg taaggtgata acgcgtgatt tcttgatgaa tgccgattgt
     2281 aaaacggcat ttggtgccat tgaagaatca ctcttatggt cagcagaaca acgggcggct
     2341 tcgctggcgg cgacgctggc ttgtcgacct aatgagggac cggtgtggat cttcggttat
     2401 ggatcgttga tgtggaatcc ggcactggag tttaccgaat tatgcaccgg tacactggtt
     2461 ggatggcatc gcgcgttttg cctgcgcctg accgccgggc gcgggactgc gcaccagccg
     2521 ggacggatgc ttgcactgaa agagggcgga cgcaccacag gcgtcgccta tcgactacca
     2581 gaagagacgc tggagcagga actaaccctg ttgtggaagc gcgagatgat taccggctgt
     2641 tatctgccaa cctggtgtca gcttgatctt gatgatggac gcacagtaaa cgccattgtg
     2701 tttattatgg acccgcgaca tccagaatat gaatctgaca ctcgcgctca ggtcatcgcg
     2761 ccgttgattg cggcggcgag cggtccgctg ggaaccaacg cacaatacct gttttcactg
     2821 gaacaggagc ttatcaaact gggaatgcag gacgatgggc tgaatgattt gctggtatcg
     2881 gtaaaaaaac tgctggcgga gaattatccg gatggtgtgt tacggccggg attcgcctga
     2941 gtaaacttcc ctcatagtgg ggcgtcagac gccccctcaa acattaaaat gtgagcactt
     3001 tatcggctga cagcgtccat tgcgccagtt ccacaagagt accgatttcc accccatcaa
     3061 taagaggaag tgtgctaatc ccgcgcccgt cggtacaggt tttgcacaat ttcaccggta
     3121 cattctgagc ggtaaggatc tccagcattt gctgaatgtt gtagccttcc cctggttttt
     3181 gcccgcgcaa cccggctgtg accgcatcag acatcaggaa cagacgcaga tccagattgc
     3241 tctcctgctc tcgtaacgca atggccagcc gcaagctgtt aaacaaggat tcgctcccgt
     3301 aaggtgcgcc attggcaacg atcacgattt tttgcattat ttactcctgt attcagggaa
     3361 ttagacactc atcttctatc ttactgcttc tgcagcgtct gaccaatcgg tcaccttttt
     3421 aaggattttc ctgaaagcgc gagaaaatac gacaaaagtt gccagtaatc gttattcttt
     3481 aaggctatgg tttttcatta ttaccggaag ttaccgacgt tttgagccgt ttcgttcctc
     3541 gcattatccc gttttattta ctcttgctgg cggcaggcgg tacagctaac gcacaatcta
     3601 ccttcgagca aaaagcggca aatccctttg ataataacaa tgatggtctg ccggatttag
     3661 gcatggctcc cgaaaaccat gatggggaaa aacactttgc tgaaattgtg aaagatttcg
     3721 gcgaaaccag tatgaatgat aacgggctgg atactggcga gcaggcaaaa gctttcgcat
     3781 tgggaaaagt ccgcgacgcg cttagtcaac aggttaatca gcacgtagag tcctggctat
     3841 caccgtgggg aaatgccagt gttgacgtca aagtggataa cgaaggacat ttcaccggca
     3901 gtcgtggaag ctggtttgtg ccgttacaag ataatgatcg ttatctcacc tggagccagc
     3961 ttggtcttac tcagcaggat gatgggttgg tgagcaatgt gggcgttggg caacgctggg
     4021 cgcgcggcaa ctggctggtg ggttataaca ctttttatga caacttgctg gacgaaaatc
     4081 ttcagcgagc gggctttggt gccgaagcgt ggggcgaata tttgcgacta tcggcaaact
     4141 tttatcagcc gtttgctgca tggcatgaac agacagccac gcaggaacaa cggatggcgc
     4201 gcgggtatga cctgacagcc cggatgcgca tgccgttcta tcaacacctc aataccagtg
     4261 tcagggtaga acagtatttt ggtgagcgtg ttgatttgtt taactctggt acgggttatc
     4321 acaatcccgt cgcgttgagt ctgggattaa attacacccc tgtgccatta gtcactgtga
     4381 cggcccagca taaacagggt gaaagtggcg aaaatcaaaa taacctcgga ctgaatctta
     4441 attaccgctt tggtgtgccg ctcaaaaaac aactttctgc gggcgaggtt gccgaaagtc
     4501 agtcgttacg tggtagtcgc tatgataatc cgcagcgaaa taatctaccg actcttgagt
     4561 accgacagcg aaaaacgtta acggtgtttc tggcgacacc gccgtgggat ctaaaacctg
     4621 gcgaaacagt gccgctgaaa ttacaaatcc gcagtcgtta cggtattcgg caactgattt
     4681 ggcagggcga tacgcagata ttaagtttga cgccgggcgc acaagccaac agcgcggagg
     4741 gctggacgct gatcatgcct gactggcaga acggggaagg ggcgagcaat cactggcgat
     4801 tgtcggtggt ggtggaagat aaccaggggc agcgtgtctc ctccaatgag atcacgctaa
     4861 cgcttgtcga accgttcgat gcattgtcaa acgacgaact gcgctgggaa ccgtaatcag
     4921 aaaatgcgct cctgatgcac ccataccgct gcttccacgc gagacttgag cttcattttc
     4981 ttcagcatgt gcttgacgtg cacttttact gtgctttcgg tgatatccag gcggcgggca
     5041 atcatcttgt tcggcaaacc ctgggcaatc agcttgagaa tatcgcgctc gcgtggggtt
     5101 aactggttaa catcgcgctc agtagtggca cggttagcgc gcaagctggc ggccagaaca
     5161 ggcgttaatg cttcgcttaa taccatttcg ccagcagctg cctgatgcaa tgctttcagc
     5221 agatcttccg gttccatatc ttttaacaga taaccatccg cgccgcgttt cagtgcggtg
     5281 accacatctt cttcatggtt agagacgctg aataccacaa tgcgccctga gagggacttt
     5341 tcgcgcagtt tatccagcgt ttccagaccg ttcatgccgg gcatattgag atctaacagg
     5401 atcagatcgg gatcaagaga ttccgccagt tcaataccct gttcgccatt actcgcttcg
     5461 ccaaccacgg tgatatctgg tgccatactg ataagctgtt ttacgccagt tcgcagcatc
     5521 gggtgatcgt caatcagcag gatagtagcc ggttcctgat tactcatggg tatctccttg
     5581 gacgtctgtg aaagtttttt cgggaataaa ggtgaccacc acttcggtgc cacctgattc
     5641 acgacggcgg acgcggcaat cgcctcgtaa actttgcgca cggtcgcgca ttattatcat
     5701 gccgtagtga ttgctgcgga tggcattttc aggcacgccg cagccgttat cctggacggt
     5761 cagtttgacc tgattatcgt tttgcgccac cgtcaccacg acttcactcg cttgcgaatg
     5821 tttgagggcg ttacttaatg cctcacgggc aatttgcaac aagtggattg cctgatgcga
     5881 aggcaccagg cgaggcggca attgataatc cagcttcacc gggaagccaa atttggcgct
     5941 gtactcttcg caactcgcct ccagcgccgg acgtaatcca ggctcggtga gctgcaagcg
     6001 gaatgtggtg agcaattcac gcaactgcgc ccaggatgca ttcagttcgt tacggatctg
     6061 acttaacagt tcgcggctgc tttctggcag cgcatcgccc tgcatctgta aacaactcac
     6121 ctgcatcttc atgcaagaga gagattgggc aatagaatca tgcagttcgc gcgcaatggt
     6181 ggcacgctct tccatcacga tcaactgttg ctgacgttcc tgatggcgat ccagcgccag
     6241 cgtggcggtg agttgttcaa ccagggtatc cagcagttgt tgttgatcat ggctaagatg
     6301 acgcccctgc ggcagggtcg ccagcaaaat accgtactgc gtatgagagt cagccagccg
     6361 ccacttcagg gtcgtgccgc gatcaccaac gggtaatacg ccgcgcgggc agagctggca
     6421 gcctttatca tcacaagtca tatctggctg gcaggtaaac tcctgatgat tctcttcatc
     6481 atcagtgtca tacacccgca attcgatatc acgtagcagg gttaaattct gtaagccgtt
     6541 gagtacaggt gacaggcgtt cacacagcgg ggcgcgggaa tgcaaacggc ggttagcctg
     6601 ccataaaaaa gagaggatct gatttttatg ctccagcccg gcggttttct cctgaacccg
     6661 ctgctcaagt acggcataac tttcggccag ttctgcagac atattgttca atgcagttcc
     6721 aagcatcgcc atttcgttgc gcccgctgat gttcgcgcgt tgggtaaaat cgcgatgact
     6781 gacggcactc gccattgcca gcagttgccg ccacggttgt agcagtcgcg cccgcaacca
     6841 gataatagtg aacaccagta aaagtgccat aaataccgcc attacccgat ggaccagtac
     6901 cactgtctca atgcgcattt ccgtggtgcg gtcaaaacca gataccagct gatcaagccc
     6961 ggcaacaaac tggctgacat ccgctgacac cgtttcgcgg ttttgtgcac gcatcagtgc
     7021 agggatcagt tcattacgcc agtaatcttg taaaccctgt aattgcgcca gttgttcgtc
     7081 tcgttctgct gctcgagtta actcggcgct aaatgccgtt tgttccatct ctttaattaa
     7141 gggcttgtct ttctcactta atggcactgc cgccaacaga cggtaacttt gcatgcgcag
     7201 cgatcccgct ttgttgatcg catgggcgct gccctgaacg ccttgcacca gccagccaga
     7261 aaccgccatc cctgccagtc caatagcagt agaaagcaac acaataagcg caacctgatt
     7321 aaccagggtg agcggagaga gacaacgttt aagcatgcaa acctcttcct tcaggcttta
     7381 aatgagcaat aaccttaatg aatgtgacga tacattctgg aatggcagta ttctcggcta
     7441 ttggctgaag tatacccata cccggaaaga gttactcctt atttgccgtg tggttagtcg
     7501 ctttacatcg gtaagggtag ggattttaca gcaccgtgaa aaatctcata atttttatga
     7561 aatcactgta ctcactatgg gtaatgataa atatcaatga tagataaagt tatcttatcg
     7621 tttgatttac atcaaattgc ctttagctac agacactaag gtggcagaca tcgaaacgag
     7681 tatcagaggt gtctatgagt cactcatccg cccccgaaag ggctactgga gctgtcatta
     7741 cagattggcg accggaagat cctgcgttct ggcaacaacg cggtcaacgt attgccagcc
     7801 gtaacctgtg gatttccgtt ccctgtctgc tgctggcgtt ttgcgtatgg atgttgttca
     7861 gcgctgttgc ggtgaactta ccgaaagtcg gttttaattt tacgaccgat cagctattta
     7921 tgttgactgc gctgccttcg gtttctggcg cgttattacg tgttccatac tcctttatgg
     7981 ttcctatctt cggtggtcgt cgctggacgg cgttcagcac cggtattctg attattcctt
     8041 gcgtctggct gggttttgcc gtgcaggata cctccacgcc ttatagcgtc ttcatcatca
     8101 tctctctgct atgcggcttt gctggcgcga acttcgcatc cagtatggca aacatcagct
     8161 tcttctttcc gaaacagaag cagggtggcg cgctgggtct gaatggtggt ctgggaaaca
     8221 tgggcgtcag cgtcatgcag ttggttgctc cgctggtggt atcactgtcg attttcgcag
     8281 tatttggtag ccagggcgtc aaacagccgg atgggactga gctgtatctg gcgaatgcgt
     8341 cctggatatg ggtgccgttc cttgccatct tcaccattgc ggcgtggttt ggcatgaacg
     8401 atcttgctac ctcgaaagcc tccatcaagg agcagttgcc ggtactcaaa cggggtcatc
     8461 tgtggattat gagcctgctg tatctggcaa ccttcggctc ctttatcggc ttctccgcgg
     8521 gctttgcgat gctgtcaaaa acgcagttcc cggatgttca gattctgcaa tacgctttct
     8581 tcgggccgtt tattggtgcg ctggcgcgtt ctgcaggtgg tgcattatct gaccgtctgg
     8641 gcggaactcg tgtcacgctg gtgaacttta ttctgatggc gattttcagc ggcctgctgt
     8701 tcctgacctt accgactgac gggcagggcg gaagcttcat ggcgttcttc gcggtcttcc
     8761 tggcgctgtt cctgacagct gggctgggta gtggttccac tttccagatg atttcagtga
     8821 tcttccgtaa actgacaatg gatcgcgtga aagcagaagg gggttctgac gaacgtgcga
     8881 tgcgtgaagc ggcaaccgac acggcagcgg cgctgggttt catctctgcg attggcgcta
     8941 ttggtggctt ctttatcccg aaagcgtttg gtagctcgct ggcattaacg ggttcgccag
     9001 tcggcgcaat gaaggtattt ttgattttct atatcgcctg cgtagtgatt acctgggcgg
     9061 tatatggtcg gcattctaaa aaataaatct taatatttga ttatcctttg cgcggcatga
     9121 tgtcgcgctt tttttatgcg tcatttagtt acaacatact catattatat ggcttatttc
     9181 gccggatttc attaagagcc attaat
//
LOCUS       DAZSBF010000161         9185 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZSBF010000161 DAZSBF010000000
VERSION     DAZSBF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.164"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHXWD_003088"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHXWD_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHXWD_003089"
     CDS             complement(1751..2509)
                     /locus_tag="ACHXWD_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR7621935.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHXWD_003090"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHXWD_003090"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR7621936.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHXWD_003091"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHXWD_003091"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR7621937.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHXWD_003092"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHXWD_003092"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR7621938.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHXWD_003093"
     CDS             complement(5474..6079)
                     /locus_tag="ACHXWD_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621939.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="ACHXWD_003094"
     CDS             complement(6082..6849)
                     /locus_tag="ACHXWD_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621940.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="ACHXWD_003095"
     CDS             complement(6852..7562)
                     /locus_tag="ACHXWD_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621941.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="ACHXWD_003096"
     CDS             complement(7578..9083)
                     /locus_tag="ACHXWD_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR7621942.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2358 c         2260 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagtacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctt
//
LOCUS       DAZSBF010000162         9095 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZSBF010000162 DAZSBF010000000
VERSION     DAZSBF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.165"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            133..669
                     /gene="hpt"
                     /locus_tag="ACHXWD_003097"
     CDS             133..669
                     /gene="hpt"
                     /locus_tag="ACHXWD_003097"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR7621943.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            complement(710..1372)
                     /gene="can"
                     /locus_tag="ACHXWD_003098"
     CDS             complement(710..1372)
                     /gene="can"
                     /locus_tag="ACHXWD_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR7621944.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1481..2407
                     /locus_tag="ACHXWD_003099"
     CDS             1481..2407
                     /locus_tag="ACHXWD_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7621945.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            2404..3174
                     /locus_tag="ACHXWD_003100"
     CDS             2404..3174
                     /locus_tag="ACHXWD_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7621946.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3279..3719
                     /locus_tag="ACHXWD_003101"
     CDS             3279..3719
                     /locus_tag="ACHXWD_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR7621947.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3783..5012
                     /locus_tag="ACHXWD_003102"
     CDS             3783..5012
                     /locus_tag="ACHXWD_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR7621948.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5016..5396)
                     /gene="panD"
                     /locus_tag="ACHXWD_003103"
     CDS             complement(5016..5396)
                     /gene="panD"
                     /locus_tag="ACHXWD_003103"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR7621949.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5670..6566
                     /gene="rpnC"
                     /locus_tag="ACHXWD_003104"
     CDS             5670..6566
                     /gene="rpnC"
                     /locus_tag="ACHXWD_003104"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR7621950.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            complement(6858..7709)
                     /gene="panC"
                     /locus_tag="ACHXWD_003105"
     CDS             complement(6858..7709)
                     /gene="panC"
                     /locus_tag="ACHXWD_003105"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR7621951.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(7721..8515)
                     /gene="panB"
                     /locus_tag="ACHXWD_003106"
     CDS             complement(7721..8515)
                     /gene="panB"
                     /locus_tag="ACHXWD_003106"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR7621952.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8628..>9095)
                     /locus_tag="ACHXWD_003107"
     CDS             complement(8628..>9095)
                     /locus_tag="ACHXWD_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7621953.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
BASE COUNT         2183 a         2144 c         2384 g         2384 t
ORIGIN      
        1 cacaattcga gctttattaa cagattccgc gaatgaatag ttttactggt atactgcgtg
       61 tcttgcgctt tgttgcggtg ccaaaacctg cccgtgcgaa gtgatttgtt tttaaatcat
      121 atggttagag atatgaaaca tactgtagaa gtaatgatcc ccgaagcgga gattaaagcg
      181 cgtatcgccg aactgggtcg tcagattact gagcgttaca aagacagcgg cagcgatatg
      241 gtgctggtgg gtctgctgcg tggctcattt atgtttatgg cggacctgtg ccgtgaagtt
      301 caggtatctc atgaagtcga ctttatgacc gcctccagct acggtagcgg catgtccacc
      361 acccgtgatg tgaaaatcct caaagatctg gatgaagata tccgtggcaa ggacgtgctg
      421 attgttgaag atatcatcga ctcggggaat acactgtcga aagtgcgtga gatcttaagc
      481 ctgcgcgaac cgaagtcgct ggcgatttgt acgctgctgg ataaaccgtc ccgtcgtgaa
      541 gtgaacgtcc cggtagaatt tatcggtttc tcgatcccgg atgagtttgt ggtgggttac
      601 ggcattgatt acgcacagcg ttaccgtcat ctgccgtata tcggcaaagt gattctgcgg
      661 gacgagtaag tgtgaagttg ccggatgtgt tgcatccggc atggcatttt tatttgtggt
      721 tggcgtgttt cagcttgagg ttggaaatcc cgtgacggta acgttgctca agggtttcgc
      781 ggttggtcgc tgtgacatcc agatcacgca gcaagccgtc gtgaatgccg taggcccagc
      841 cgtgaatggt aactttctgc ccgcgtttcc acgctgattg cataatggtg gagtggccta
      901 ggttataaac ctgttccatg acgttcagtt cacacaaggt atccagacgg cgctcttgcg
      961 gcatttcgcc gagcaatgag ctatgtttga accagatatc gcggatatgc agcagccagt
     1021 tgttgataag ccccagttcc gggttttcaa cggcggcttg tacgccgccg caaccgtagt
     1081 ggccacagat aataatgtgt tcaacttcga gtacatccac tgcatactga accacggaaa
     1141 ggcagttcag gtcggtgtga atgaccaggt tagcaacatt acggtgaaca aagagttcgc
     1201 ccggctcaag accggttaaa cgttctgcag gaacgcgact gtcggaacat ccaatccata
     1261 gaaagcgcgg tttttgcgct tgtgccagtt tctcaaaaaa cccgggatcc tcttccacca
     1321 gcatttttga ccatagtgca ttgttgctga tgagtgtatc tatgtctttc atggaggtta
     1381 acgacctgta accaaataat tacgtttggc taatataggg caactccggg acgatttaaa
     1441 ccacagataa agtgtaagaa cgtaaggtaa gtaaaaattt atgaccattg cactggaact
     1501 tcaacagctt aaaaaaacct atccaggcgg cgttcaggcg cttcgtggaa tagatttgca
     1561 ggtcgaagcg ggtgattttt atgcgcttct cgggccgaac ggggccggga aatcgaccac
     1621 tatcggtatt atcagctctc tggtaaataa aacctccggg cgggtcagcg tatttggtta
     1681 cgatctcgag aaagatgtcg tgaacgctaa acgtcagttg ggactggtgc cgcaggaatt
     1741 taacttcaac ccgtttgaaa ccgtgcagca aattgtggtg aatcaggcag ggtactacgg
     1801 tgtggagcgc aaagaggcgt acatccgcag cgaaaaatat cttaaacaac tcgatctatg
     1861 gggaaaacgc aacgaacgtg cgcgtatgtt atctggcggg atgaagcgcc gtttaatgat
     1921 tgcccgtgcg ttaatgcatg aacctaaact actgattctc gacgaaccga ccgcaggcgt
     1981 ggatattgaa cttcgccgct caatgtgggg ctttttgaag gatttaaacg acaaaggcac
     2041 caccatcatt ctcaccacac actacctgga agaagcagaa atgctgtgcc gcaatatcgg
     2101 cattattcaa cacggtgagc tggtggaaaa tacctcgatg aaggcgctgc tggcgaagct
     2161 gaaatcggaa acctttattc tcgatctcgc accgaaaagt ccgttaccga agctcgatgg
     2221 ctatcagtat cgactggtcg ataccgcgac gctggaagtt gaagtgctgc gtgagcaggg
     2281 gatcaacagc gtatttacgc agttaagtga gcagggcatt caggtattaa gtatgcgtaa
     2341 caaagctaac cgtctggaag agctgtttgt ttcactggtt aatgaaaaac aaggagatcg
     2401 cgcatgatgc atctttactg ggtggcgcta aaaagcatct gggcgaaaga gatccatcgc
     2461 tttatgcgta tctgggtgca gacgctggtg ccgccagtca tcaccatgac cctttacttt
     2521 attatcttcg gtaacctgat tggttcgcgt attggcgata tgcatggctt cagctatatg
     2581 cagttcatcg taccggggct gatcatgatg tcggtgatca ccaatgccta cgccaacgtt
     2641 gcgtcatcat tttttggtgc caagttccag cgtaatattg aagagctgct ggtagcgccg
     2701 gttccgactc acgtcattat tgccggatat gtcggcggtg gcgtggcgcg tggtctgttt
     2761 gttggcattc tggtgacggc aatttcactg ttttttgtgc cgtttcaggt gcattcgtgg
     2821 gtattcgttg ccttaaccct ggtgctcacg gcggtgttgt tctcccttgc gggtttgctg
     2881 aacggtgtgt ttgccaaaac gttcgatgac atcagcctgg tgccaacctt tgtgttaacg
     2941 ccactcacgt atttgggtgg ggtcttttac tcactgactt tgttgccgcc gttctggcaa
     3001 gggctgtcgc acctgaaccc aatcgtttat atgatcagtg gtttccgcta cggcttcctc
     3061 ggtatcaatg atgttccgct ggtcactacc tttggcgtac tggtggtctt tattgtggcg
     3121 ttttatttga tctgttggtc gctgatccaa cgtggacgtg gtttgcgtag ctaaggctat
     3181 ttcctctcct ctggatttgg gggagaggag ttttgacggc tatcaccctt tatcaacaat
     3241 ggtcagggta gactgatttt cggctaagga ggaaggcgat gttaggttgg gtaattacct
     3301 gtcacgatga ccgggcgcaa gagatactgg atgcgctgga gaaaaaacat ggggcgcttc
     3361 ttcagtgccg ggccgtgaat ttctggcgtg ggttaagctc taatatgctc agccgcatga
     3421 tgtgcgatgc tctgcatgaa gcggactctg gtgagggtgt catcttctta accgatatag
     3481 ccggagcgcc accgtaccgc gtggcttcat tattaagcca caaacactcc cgttgcgaag
     3541 tgatttctgg tgtcacgtta ccgttaattg aacagatgat ggcttgccgt gaaaccatga
     3601 ccagttcaga gtttcgcgag cgtattgtcg aactgggtgg gccggaggtg agtagtcttt
     3661 ggcaccaaca acaaaaaaat ccgcctttcg tcctcaaaca taatttgtat gagtattaac
     3721 ccacgattct tatagcgctt ttgctacaat aaaagcgttg tttcaccctc ggttattttt
     3781 tcatgtacaa acaagctgtt attctcctgc tgatgctgtt taccgcaagt gtcagtgccg
     3841 cgttacctgc ccgttatatg caaaccatcg aaaatgctgc ggtctgggcg caaattggcg
     3901 acaagatggt gaccgtgggg aatattcggg ccggacaaat cattgccgtg gagcccactg
     3961 ccgcaagtta ttacgcattt aattttggct ttggtaaagg gtttatcgat aaagggcatc
     4021 tcgagccggt tcaggggcga caaaaagttg aagacggttt gggtgacctc aacaagccgc
     4081 tgagtaatca gaacttagtt acctggaaag atacgccggt ctataacgcg ccgagtgtgg
     4141 gcagtgcgcc atttggggta ttggcggaca atttgcgcta cccgattttg cataaactga
     4201 aagacaggtt aaatcaaacc tggtatcagg tccgtattgg cgatcgactg gcctatatca
     4261 gcgcgctgga tgcccaaccc gataatggcc ttccggtgct aacttatcac catattctgc
     4321 gcgacgaaga aaacacccgt tttcgccata cttcgacgac cacctcggta cgcgctttca
     4381 ataaccaaat ggcctggctg cgcgacaggg gatacgcgac actgagcatg gcgcagctgg
     4441 aaggttacgt gaagaataag atcaatctcc ctgcgcgagc ggtggtgatt acctttgatg
     4501 atggcctcaa gtcggtgagc cgctatgcgt atcctgtatt gaaacaatat ggcatgaagg
     4561 cgacggcgtt tgttgttacc tcgcgcatca aacgtcaccc gcagaagtgg aacccaaaat
     4621 cgctgcaatt tatgagcgtt tctgagctta acgaaattcg cgatgttttt gattttcagt
     4681 cacataccca ttttttgcat cgggtggatg gctatcgccg acccatatta ctgagccgta
     4741 gtgagcacaa tattctgttt gattttgcac gttcacgccg cgctctggcg caatttaatc
     4801 cgcatgtctg gtatctttcg tatccgtttg gcggatttaa tgacaaagcc gtgaaggcag
     4861 caaacgatgc cggatttcac ctggcggtga caaccatgaa aggcaaagta aaaccggggg
     4921 ataatccgtt gttactaaaa cgactttata tcttaagaac ggattcgctg gagacgatgt
     4981 cgcggctggt gagtaaccag ccgcagggat aacaatcaag caacctgtac cggaatcgct
     5041 ttcgcggtac gtttcatttc attgtcgcct tcaaaatagg cgacgttggg tcgccaggtg
     5101 cgagcttctt catctggcat ggtaacgaag ctggcgatga tgacaatatc gccgacactg
     5161 gcgcagtggg ccgccgcacc gttaacagaa ataattctcg aaccgcgttc tgccgcgatg
     5221 gcataagtgg agaaacgctt gccgttggtg acattccaga tatcaatggc ttcgttttcg
     5281 agaataccgg ctgcgtcaag aaaatcctgg tcaatggcgc aagaaccttc atagtgcagg
     5341 tccgcatgag tcactttcac gcggtggagt ttgccctgca gcatcgtgcg aatcataact
     5401 tctacctttc taccctgtcg ttaacgaagc aggcgatgcc tgctttgagg aaattctcac
     5461 gcagtattgc ccgatttttg tttagtgtct actcatctga cggcatttac atcagcagtt
     5521 tgcgtaccgt gccgaagcgt gccatgtaac ggctgtaacc aattgaaatt tctctgaacg
     5581 ctgctcggta aacactaaag aggtggctga cgatggcacg ccttgactgg atactgcctt
     5641 cacgcaagac aacacggaag gcagggaaga tggatgcacc gagtaccaca ccgcatgatg
     5701 cagtgtttaa acaattttta atgcacgcgg agacggctcg cgactttctg gagatacatt
     5761 tgccagtgga attacgagaa ctttgtgacc tcaacacgct tcatttagag tcggggagtt
     5821 tcattgaaga gagcctgaaa ggacacagca cggacgtgct ctattccgtg caaatgcagg
     5881 gcaatcccgg ttatctgcat gttgtgattg aacaccaaag caagccggat aagaaaatgg
     5941 tctttcgcat gatgcgttat tctattgctg ccatgcaccg gcatctggag gcggatcacg
     6001 ataagctgcc gctggtggtg ccgatactgt tttatcaggg cgaggccaca ccttatccgc
     6061 tctcaatgtg ctggtttgat atattttact cgccggagct ggcacgacgc gtctataaca
     6121 gtcctttccc gctggtggat atcaccatca caccggatga cgaaatcatg caacatcggc
     6181 ggattgcgat tctcgaacta ctgcaaaaac atattcgcca gcgcgactta atgttattgc
     6241 tggagcaact ggtcacgctg attgacgaag ggtacactag cggaagtcag ttagttgcca
     6301 tgcaaaacta tatgctgcaa cgcggtcata ctgaacaagc ggatttgttt tatggtgtgc
     6361 tgagagacag ggaaacggga ggggagtcta tgatgacgct ggcgcagtgg tttgaagaga
     6421 aagggattga gaaggggatt cagcagggaa gacaggagga aaggcaagaa ttcgcccagc
     6481 gttttctgag taaagggatg tctcgggaag acgttgcaga gatgacaaat ttatctcttg
     6541 ctgagattga taggctgatt aactaagttg tctgcgttat gaataattgc ttacagacgc
     6601 catcgccatg acgtggggca aattaataat tggcgccatc agccgtagcg ccagttatac
     6661 tcgtcatact ttaaattgca tgtgctgcgt ctgcgttcgc taaccccagt cacttactta
     6721 tgtaagctcc tggggattaa tgagagacat ccatgtctct caccgaaggc cagcctgcgg
     6781 ctgggcaaat tcgttcctga cgaatttgtc actcgcttgc cgccttcctg caacttgaat
     6841 tatttagagt aaaagcatta cgccagttcg acgattttgt tgtcgatcag gcgggcatcg
     6901 ccaagccagg cggctaccag aattacagct cgtttgctgg tttcagagac ttccagcaac
     6961 gtgtcggcat cgcgaatctg aatatcatcg gcgcggaagc ctttttcatt cagttcttgc
     7021 cctgcaatgg caataatttc atcaagatcc cgctcgccag cctgcaattt gtcggcaatt
     7081 gaacttaaaa ctttgtacag accgggcgca attttgcgtt gttccgccgt cagataacca
     7141 ttacgggaac tgagcgcaag gccatctttg gcgcgcataa tcgggacgcc gacaatttca
     7201 atatcgaagc ccatatcggc aaccattttg cggattagcg ccagttgctg aaagtccttt
     7261 tcaccgaagc aggcgatgtc cggctggacc aggttaaaca gcttgctgac gatagtcgaa
     7321 acgccgcgaa aatgccccgg acggctcgcg ccttccagca tggtcgaaag cccgggaacg
     7381 tcaacgtaag tatgggtttc agtaccgttc gggtagatct cttttaccga aggggcgaaa
     7441 actaaatcca ctttacgttt gtttagcttc tcgcagtcct cctgcaaggt ccgtggataa
     7501 cgtgccagat cttccgggcg gtcgaactgc atcgggttaa cgaaaatact gacgacgacc
     7561 acatcggcgc gggctttggc ttcgtcgacc agcttcatat ggccatcgtg cagattaccc
     7621 atagtaggca ccagcgccac gcgcttgcct tccatacgca ggcggcgaat ttgctgacgc
     7681 agcagcggca gggtttcgat aattaacaca acgtgactcc ttaatggaaa ctgtgttctt
     7741 cgcccggata aacgccggac tccacttcag ccatatactg ccgcacagcc gcgcggatgt
     7801 cgcccgtttc ggcgaggaaa tttttagcga atttaggaat gtgaccgccg gtaatgccga
     7861 aggcgtcgtg catcacgagg atctgcccgt cagtgacgtt gcctgcgcca atgccaataa
     7921 ccgggatcgc cagtgcttcg gtaatacgtt ttgccagttc aatcggcacg cattccagca
     7981 ccagcagctg tgccccagcg gcttctaagg ctaatgcatc gctgagcagt tgatcgcccg
     8041 cttcatcgcc gcgcccctga actttgtagc caccgaaaat attcactgac tgtggggtta
     8101 aacctaagtg accacataca ggaacggcac gttcggtcag catttttacg gtttcgacca
     8161 gccactcacc gccctcaatt ttgaccatat tggcaccggc acgcataacc gttgcggcgt
     8221 tttcaaaggc ttgttccggc gtggcatacg ccataaacgg caggtcagcc aacagcaggc
     8281 agtttggcgc accacgacgt acggaggcag tgtggtaggc gatatcggca acggtcaccg
     8341 gcagggtgga gtcgtgaccc tgaaccgtca tgcccagcga gtcgcctacc agcatgacgt
     8401 taagtccttc atcagcaaat agtttcgcga agctgtagtc gtaagcggtg atggtcgcga
     8461 aacgcttttt atcctgctta catttctgaa gtgaagcaat ggtggtcggt ttcataacgt
     8521 gtcctgataa atgatgtcgt gttgcctggc attttatcag tcacattggt gggggcaatg
     8581 atttatcaat taccgcaccg ccagatgatg gcggtgcggt ggacttatta agggtaggtt
     8641 acctggaaag tactggtggc tttaaactca ccagcttcta ttggtatagt tccatcctgt
     8701 tgtagcgtgg cctggaaatg gagagggtat gttatacctg tatcttgatc cgggtaaact
     8761 cctcctgttg tatcgtcttt gcctctcggg tcgtaatctt tataaacaga atttgaatca
     8821 ttaggtttca atgtcattag aggattttta ctagttgcta aaccttctat gagtacaccg
     8881 actccttttg ctgcgtcatt acctgtgaga gtattaccaa ggagttgccc gttttcagta
     8941 ccaacctttg ttgaaactaa ttttgtctca atattagtca cacgaacgca gttttgaagt
     9001 gaaatatcaa aaggaaccgg tgtggcaccg tttctgattt gttctgcact atattcaccc
     9061 atttttaccg tagaaccagt cacagaagtt ccact
//
LOCUS       DAZSBF010000163         9072 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZSBF010000163 DAZSBF010000000
VERSION     DAZSBF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.166"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHXWD_003108"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="ACHXWD_003108"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR7621954.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHXWD_003109"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="ACHXWD_003109"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR7621955.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHXWD_003110"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="ACHXWD_003110"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR7621956.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHXWD_003111"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="ACHXWD_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR7621957.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHXWD_003112"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="ACHXWD_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR7621958.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHXWD_003113"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="ACHXWD_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR7621959.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHXWD_003114"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="ACHXWD_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR7621960.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHXWD_003115"
     CDS             8188..8256
                     /gene="ykiE"
                     /locus_tag="ACHXWD_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR7621961.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8343..9040
                     /locus_tag="ACHXWD_003116"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="ACHXWD_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAZSBF010000164         8986 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZSBF010000164 DAZSBF010000000
VERSION     DAZSBF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.167"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            124..1522
                     /gene="ulaA"
                     /locus_tag="ACHXWD_003117"
                     /pseudo
     CDS             124..1522
                     /gene="ulaA"
                     /locus_tag="ACHXWD_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1538..1843
                     /gene="ulaB"
                     /locus_tag="ACHXWD_003118"
     CDS             1538..1843
                     /gene="ulaB"
                     /locus_tag="ACHXWD_003118"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR7621962.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1853..2317
                     /gene="ulaC"
                     /locus_tag="ACHXWD_003119"
     CDS             1853..2317
                     /gene="ulaC"
                     /locus_tag="ACHXWD_003119"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR7621963.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2331..2981
                     /gene="ulaD"
                     /locus_tag="ACHXWD_003120"
     CDS             2331..2981
                     /gene="ulaD"
                     /locus_tag="ACHXWD_003120"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR7621964.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2991..3845
                     /gene="ulaE"
                     /locus_tag="ACHXWD_003121"
     CDS             2991..3845
                     /gene="ulaE"
                     /locus_tag="ACHXWD_003121"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR7621965.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3845..4531
                     /gene="ulaF"
                     /locus_tag="ACHXWD_003122"
     CDS             3845..4531
                     /gene="ulaF"
                     /locus_tag="ACHXWD_003122"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR7621966.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4628..5179
                     /locus_tag="ACHXWD_003123"
     CDS             4628..5179
                     /locus_tag="ACHXWD_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR7621967.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5254..5569)
                     /locus_tag="ACHXWD_003124"
                     /pseudo
     CDS             complement(5254..5569)
                     /locus_tag="ACHXWD_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            5896..6291
                     /gene="rpsF"
                     /locus_tag="ACHXWD_003125"
     CDS             5896..6291
                     /gene="rpsF"
                     /locus_tag="ACHXWD_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR7621968.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6298..6612
                     /gene="priB"
                     /locus_tag="ACHXWD_003126"
     CDS             6298..6612
                     /gene="priB"
                     /locus_tag="ACHXWD_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR7621969.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6617..6844
                     /gene="rpsR"
                     /locus_tag="ACHXWD_003127"
     CDS             6617..6844
                     /gene="rpsR"
                     /locus_tag="ACHXWD_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR7621970.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6886..7335
                     /gene="rplI"
                     /locus_tag="ACHXWD_003128"
     CDS             6886..7335
                     /gene="rplI"
                     /locus_tag="ACHXWD_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR7621971.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7447..8418)
                     /locus_tag="ACHXWD_003129"
     CDS             complement(7447..8418)
                     /locus_tag="ACHXWD_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR7621972.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8471..8887)
                     /locus_tag="ACHXWD_003130"
                     /pseudo
     CDS             complement(8471..8887)
                     /locus_tag="ACHXWD_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
     gene            complement(8904..>8986)
                     /locus_tag="ACHXWD_003131"
     CDS             complement(8904..>8986)
                     /locus_tag="ACHXWD_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR7621973.1"
                     /translation="KSLSFSKSVELHDKVIGYYLNIKHYQ"
BASE COUNT         2165 a         2197 c         2382 g         2242 t
ORIGIN      
        1 tttgcgggtc gcgtcacatt taatcataaa taatcttgtt gtgattactt ttgaaaatta
       61 gagtgagtgc acaacattcc gggtgtgcgg aatacccggt tacctcttct tcaggagatc
      121 gttatggaga tcctctacaa catctttacc gtgtttttta accaggtcat gaccaatgcc
      181 ccgttgttgc tgggtattgt gacctgtctg ggctacatcc tactgcgcaa aagtgtcagc
      241 gttattatta aaggcacgat taaaaccata attggtttca tgttgttgca ggcagggtcc
      301 ggcatcctca ccagcacctt caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt
      361 aaacggcgca atttccgata cttacgcgtc gatgatggca accatcgacc gcatgggcga
      421 tgcctatagc tgggtaggtt acgcggtgtt gttagcgctg gcgctgaaca tctgttacgt
      481 gctgctgcgt cgcattaccg gtattcgtac catcatgctg actggtcata tcatgttcca
      541 gcaggcgggg ctgattgccg ttacgttgtt tattttcggc tactccatgt ggacaaccat
      601 tatctgcacg gcgattctgg tttcgctcta ctggggcatt acctccaaca tgatgtacaa
      661 gccgactcag gaagtgacgg acggctgcgg tttctccatc ggtcaccagc aacagtttgc
      721 atcatggact gcctataaag ttgcgccgtt cctcggcaaa aaagaggaaa gcgttgaaga
      781 cctcaaactg ccaggctggc tgaatatttt ccacgacaac atcgtctcca cggcgattgt
      841 catgaccatc ttctttggtg ccattctgct ctccttcggt atcgacaccg tgcaggcgat
      901 ggcaggcaaa gtgcactgga cggtctatat cctgcaaacc ggtttttcct tcgcggtggc
      961 gatcttcatc atcactcagg gtgtgcgcat gtttgtggcg gaactctctg aagcgtttaa
     1021 cggtatctcc cagcgcctga tccctggtgc ggttctggcg attgactgtg cggctattta
     1081 tagcttcgcg ccgaacgccg tggtctgggg ctttatgtgg ggcaccatcg gtcagctgat
     1141 tgcggttggc atcctggtcg cctgcggttc ctcgagcctg attatcccag gctttatccc
     1201 gatgttcttc tccaacgcca ccatcggcgt gttcgctaac cacttcggcg gctggcgtgc
     1261 ggcgctgaag atttgtctgg tgatggggat gatcgaaatc tttggttgcg tctgggcggt
     1321 gaaacttacc ggtatgagtg cctggatggg gatggcggac tggtcgattc tggcaccgcc
     1381 gatgatgcag ggcttcttct ccatcggtat cgcctttatg gccgtcatca ttgtaattgc
     1441 actggcttat atgttcttcg ctggccgcgc actgcgcgca gaagaagatg cagaaaaaca
     1501 actggcagaa cagtctgctt aataaggagt tttgattatg accgtacgta ttctggctgt
     1561 gtgtggcaac ggacaaggca gttccatgat catgaggatg aaagtggacc agtttttaac
     1621 ccaatcaaac attgaccaca cggtaaatag ctgcgcggtt ggcgaataca aaagcgagtt
     1681 gagcggcgcg gatatcatca tcgcttctac gcacattgcg ggcgaaatca ccgtgaccgg
     1741 caacaaatac gtggttggcg tgcgcaacat gctctctcct gccgactttg gcccgaaact
     1801 gctggaagtg atcaaagcgc atttcccgca ggatgtgaag taaggacgcg ccatgaaatt
     1861 acgtgattcg ctggcggaga ataaatccat ccgcctgcag gctgaagctg agacatggca
     1921 ggacgcagta aaaatcggcg ttgacctgct ggtggcggcg gatgttgtcg agccgcgtta
     1981 ctaccaggcg attctggatg cggttgaaca gcacggtccg tactttgtgc tcgcaccagg
     2041 cctggcaatg ccgcacggtc gcccggaaga gggcgttaag aaaaccggtt ttgcgctggt
     2101 gacgctgaaa aagccgctgg agttcaacca cgaagataac gacccggtgg atatcctcat
     2161 caccatggcg gcggtcgatg ccaacacgca ccaggaagtg ggcatcatgc agatcgtcaa
     2221 cctgtttgaa gatgaagaaa atttcgaccg tttacgtgcc tgccgtaccg agcaggaagt
     2281 actggatctc attgaccgca ccaacgcggc agcttaagaa ggaattgaag atgtcattac
     2341 cgatgttgca agtcgcgctg gacaaccaga ctatggatag cgcctacgaa accactcgcc
     2401 tgattgccga agaagtcgac attatcgaag tgggtaccat tctgtgcgtg ggcgaaggcg
     2461 tgcgtgcggt tcgcgatctg aaagcgctct acccgcacaa aatcgtgctg gcagacgcca
     2521 aaattgccga tgcgggcaaa attctttctc gtatgtgctt cgaagccaac gctgactggg
     2581 tgaccgtgat ttgttgtgcg gatatcaaca ccgccaaagg tgcgctggac gtagcaaaag
     2641 agtttaacgg cgacgtgcag atcgaactga ccggttactg gacctgggaa caggcgcaac
     2701 agtggcgcga cgcaggtatt cagcaggtgg tttatcaccg cagccgtgac gcgcaggccg
     2761 ctggcgtggc gtggggtgaa gccgatatca ctgcgatcaa acgtctttcc gatatgggct
     2821 tcaaagtcac cgtcaccggt ggcctggcgc tggaagatct gccgctgttc aaaggcattc
     2881 caattcatgt gtttatcgcc ggtcgcagca tccgtgatgc cgcttctccg gttgaagccg
     2941 cgcgtcagtt caaacgttcc atcgccgaac tgtggggcta aggagcggat atgttgtcca
     3001 aacaaatccc gcttggcatc tatgaaaaag cgctccccgc cggggagtgc tggctggaac
     3061 gcctgcgact ggcaaaaacg ttgggcttcg attttgtcga gatgtcggta gatgaaactg
     3121 acgagcgcct gtcgcgcctc gactggagcc gcgagcagcg tctggcgctg gtgaatgcga
     3181 ttgttgaaac cggcgtgcgt gtgccgtcca tgtgcctttc tgcccatcgt cgtttcccgt
     3241 tgggcagcga agatgacgcg gtgcgggcgc aggggctgga gattatgcgt aaagccatcc
     3301 agttcgccca ggacgttggc attcgcgtga tccagctggc gggctatgac gtttactatc
     3361 aggaagccaa taacgaaacg cgtcgtcgtt tccgtgacgg cctgaaagag agcgttgaga
     3421 tggcgagccg cgcgcaggtg acgctggcga tggagatcat ggattatccg ttgatgaact
     3481 ccatcagcaa ggcgctgggt tacgcgcact atctcaacaa cccgtggttc cagctctacc
     3541 cggatatcgg caacctgtcg gcgtgggaca acgatgtgca gatggagttg caggccggaa
     3601 tcgggcatat cgtcgcggtg catgtgaaag acaccaaacc gggcgtcttc aaaaacgtgc
     3661 catttggcga aggtgtggtg gatttcgaac gttgcttcga aacgctcaaa cagagtggct
     3721 attgcgggcc gtacctgatt gagatgtgga gcgaaacggc ggaagacccg gcggcagaag
     3781 tggtgaaagc gcgtgattgg gtgaaagcgc gcatggccaa agcaggcacg gtggaggcgg
     3841 cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc cgcgctatgg
     3901 gctggtgacc tttacctggg gcaacgtcag cgctatcgac cgcgaacgcg ggctggtggt
     3961 gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg tggtggtcga
     4021 tatgagcggc aaggtggtgg aaggggagta tcgcccgtct tccgacaccg cgacgtatct
     4081 cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact ccactcatgc
     4141 tactgcatgg gcgcaggcgg ggctggcgat cccggcgtta ggcactacgc acgccgacta
     4201 cttctttggc gacatcccgt gtacgcgcgg attaagcgaa gaagaggtgc agggcgagta
     4261 tgaactgaac accggcaaag tgattatcga aacgctgggt gacgccgagc cgctgcatac
     4321 gccggggatt gtggtgtatc agcacggtcc gttcgcctgg gggaaagatg cccacgacgc
     4381 ggtgcataac gcggtggtga tggaagaagt ggcgaaaatg gcgtggattg cgcgcagtat
     4441 taacccacaa ctgaatcaca tcgacagctt cctcatgaat aaacacttca tgcgtaagca
     4501 cggtcctaac gcctattacg ggcagaagta agcagtgcgc cccggaatta ttacctcctg
     4561 cgggatgtaa ttggtctgat tccggggccg cttcagttaa aaaatcgtat aaacaacagg
     4621 tgtttttatg gctatcaatg gattgtttgt cagaacggat ccgcaacgtc gccgctatgg
     4681 cgttgcgtta cttgtgggtg tactttcagg aattatttca gcatttgtta aatggggagc
     4741 ggaagtcccc ttgccaccgc gcacattttc cggtggtcgc gatgaattta acccgccgta
     4801 tatctttcta cgggattatt taggtatcga cccaacccaa actgtgtata cattctcgga
     4861 acatattatc aacccggtga tggtgacgca tatcatcttc tcactggttt ttgcgatagg
     4921 gtattgtatt gtcgcggaaa ttttcccgaa agtgaaatta tggcagggga tattagcagg
     4981 gcttattgtg acagtggcag tccacggtat attttgcccg gcgctgaatt taacgccgcc
     5041 gctcactcaa ttgccttttg acgaatatgc ctctgaaatc ctggggcata ttttctggtt
     5101 ctggataata gaaattatgc gccgcgactt acgtaatcgc attactcatg agccagatcc
     5161 ggaagtcgca atccggtgaa taaccgcata aaaaagcccc ctgcgaaggg ggcaaagcaa
     5221 actatggcaa tgtttcgttg gttatacctg gtgctagcga taaatatccg cgctggcgtg
     5281 catattgccg ttactccccg gttcccgcat cagaattacg tggtagtacg ttactccccg
     5341 gttcccgcat cagaattacg tggtagtacg ttgcgccctg cgcatctgtc tcttcattta
     5401 atgcctgacg cgcttcgctt tcagtggcga aattatgatt gatataaatc acgcctaagc
     5461 tttgcacatc atccatgttt ctggcctggt ggttattaat ttcaatggct gcccatgtat
     5521 ttgcacttag caaaagcaca gccagaaggg ctaaaactcg actgaacata gatacctcct
     5581 cgacggctga ctttgtgtgc tctcctctgt gatgatcttc tgatttaatt ttaatcaatg
     5641 ataaagaagt tgatggtgac catttctgat gcagttgttc aaaaaaacac catgatgaag
     5701 tgtgatgaac tccaaatcag cggtttagag gttaattgcg aaaggggaga tttatttcgg
     5761 ctctgccctt gagtttagcg gggcatacaa gtactataac gcgtcatttt tcagccgacc
     5821 tttaacacgt tccttgcctc cccgggattc ggctgaccca gacaggaggc tgaataatcc
     5881 gtaaggagca attcgatgcg tcattacgaa atcgttttta tggtccatcc tgaccagagc
     5941 gaacaggttc cgggcatgat cgagcgctac actgctgcca tcactggtgc agaaggcaag
     6001 atccaccgtc tggaagactg gggccgccgt cagctggctt acccgatcaa caaactgcac
     6061 aaagcacact acgttctgat gaacgttgaa gctccgcagg aagtgatcga tgagctggaa
     6121 actaccttcc gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac caagcacgct
     6181 gttaccgaag catctccgat ggttaaagcg aaagacgagc gccgtgagcg tcgcgatgat
     6241 ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt ctgaagagta atttctgatg
     6301 accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc cccttcgaaa ggtcagtcca
     6361 tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga ggaagccggt
     6421 tttcaccggc aggcgtggtg ccaaatgccc gttattgtta gcggacacga aaaccaggcc
     6481 attactcaca gtataacggt cggcagtcgc ataaccgttc aggggttcat ttcatgccac
     6541 aaggcaaaga acggactgag caaaatggtt ttgcatgccg agcagattga attgatagat
     6601 tctggagact agccatatgg cacgttattt ccgtcgtcgc aagttctgcc gtttcaccgc
     6661 ggaaggcgtt caagagatcg actataaaga tatcgctacg ctgaaaaact acatcaccga
     6721 aagcggtaag attgtcccaa gccgtatcac cggtacccgt gcaaaatacc agcgtcagct
     6781 ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg ccgtacactg atcgccatca
     6841 gtaatcggtc acggtccatt aatacgactt tgagaggata aggtaatgca agttattctg
     6901 cttgataaag tagcaaacct gggtagcctg ggtgatcagg taaacgttaa agcgggctat
     6961 gctcgtaact tcctggtacc gcagggtaaa gctgttccag ctaccaagaa aaacattgaa
     7021 ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct ggcagctgct
     7081 aatgctcgcg ctgagaaaat caatgcactg gaaactgtta ccatcgcgtc taaagctggc
     7141 gacgaaggta aactgttcgg ttccatcggt actcgcgaca tcgctgacgc tgtaactgca
     7201 gctggcgttg aagtggctaa gagcgaagtt cgtctgccga acggcgttct gcgtaccact
     7261 ggcgagcacg aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt gatcgtaaac
     7321 gtagtagctg aataattcgt tattcaacga gacgtaaaaa cgccgaccag tggtcggcgt
     7381 tttgcttttg tagcgagcac ttgcagatat cgataagggc cacaataagc agccctacga
     7441 agataattac ttagctgcct gaatatcagc cagcattggg gcaagggctg ggttgtcctt
     7501 agcgaagttt gcgtacattg gctgtacttt ctcgataaac ggagctttat ctacctggta
     7561 gactttcacg cccattttat ccagttttgc aaattcttgt ttctcttcct cgctccacaa
     7621 gtctttcatg tagctcattg attctttagc tgcttttaat ataactgcct gattttccgg
     7681 gctaagttta tcaaacgcag cattgctcat cagaaccaca tcgggaacca tagtgtgttc
     7741 gtcaaggctg aaggattttg ccacttcacc gtgacgcatg gtggtcagcg ccatcacgct
     7801 gttttctgcc atatcgacca caccctgctg taaggctgta tacaactcgc cgtaatccag
     7861 tggcgtcggg ttaccgccca tgactttgat catttcaact gcgccagggc ttggctggac
     7921 acggactttc atgcctttca gatcgtctgg tttcagtact ggtttattgc catagaaact
     7981 gcgggcgcct ccatcataaa aagtcagccc gaaataacct ttgcttttcg ttgagtcgag
     8041 gatttttctc ccgatatcgc cctgcatgac ctgataataa tgatcacgat cgcggaacaa
     8101 ataaggcagg ctaaataatt tataggaatt ttcaaatgct tccatttctg ccgcattggt
     8161 ttttaccagt ggaatagcgc cagaacgaat cagctccatt gtttctcgtt gagtacccaa
     8221 tgttccattt ggataaatac gaatagtaat atcaccgtta gagtactctt tgttcttttt
     8281 cgcaaagaac tccatcgctt tatgaacagg atgagactta tcctgattat gactcagttt
     8341 taatgtcact gcccaggact ggcttgcgga taacataaga acagcaccga taatgccagc
     8401 ggatactttt attttcatag atgttccttt tctaataaat atggcggcag gtgccgccag
     8461 tgaatgcgca tcagtagccc agtgcttttg gcaatgccat actgatttgc ggaatatagg
     8521 tcaccattgc cagcaccaat accatcacgg catagaatgg cagcataggt ttgataattt
     8581 tattaatcgg taatttagat acactacaac cgacaaataa cacactgcct acaggcggag
     8641 tacaaatacc gatacacatg ttcagtacca tgacaatacc aaagtgaatc gggtccatgc
     8701 ccagttcggt gacaacaggt aaaaatatcg gcgtaaaaat cagtaccgcc ggtgtcatat
     8761 ccataaaagt gccgataatc aacaggataa tattgataat aagcaggatt attattgggt
     8821 tgtcagaaat agtgttaagc aaatcgagaa tcaggaaggg aacgtcagcg tttgacattg
     8881 cccatgacgg taatgactcc aacttattga tagtgtttta tgttcagata atacccgatg
     8941 actttgtcat gcagctccac cgattttgag aacgacagcg acttcc
//
LOCUS       DAZSBF010000165         8963 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZSBF010000165 DAZSBF010000000
VERSION     DAZSBF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.168"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            241..1128
                     /gene="rhtA"
                     /locus_tag="ACHXWD_003132"
     CDS             241..1128
                     /gene="rhtA"
                     /locus_tag="ACHXWD_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR7621974.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1427..1930
                     /gene="dps"
                     /locus_tag="ACHXWD_003133"
                     /gene_synonym="pexB"
     CDS             1427..1930
                     /gene="dps"
                     /locus_tag="ACHXWD_003133"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR7621975.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2334..3080
                     /gene="glnH"
                     /locus_tag="ACHXWD_003134"
     CDS             2334..3080
                     /gene="glnH"
                     /locus_tag="ACHXWD_003134"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR7621976.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3219..3878
                     /gene="glnP"
                     /locus_tag="ACHXWD_003135"
     CDS             3219..3878
                     /gene="glnP"
                     /locus_tag="ACHXWD_003135"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR7621977.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            3875..4597
                     /gene="glnQ"
                     /locus_tag="ACHXWD_003136"
     CDS             3875..4597
                     /gene="glnQ"
                     /locus_tag="ACHXWD_003136"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR7621978.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            4714..6939
                     /gene="ybiO"
                     /locus_tag="ACHXWD_003137"
     CDS             4714..6939
                     /gene="ybiO"
                     /locus_tag="ACHXWD_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR7621979.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(6936..7862)
                     /gene="rlmF"
                     /locus_tag="ACHXWD_003138"
     CDS             complement(6936..7862)
                     /gene="rlmF"
                     /locus_tag="ACHXWD_003138"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR7621980.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8138..8398
                     /gene="mcbA"
                     /locus_tag="ACHXWD_003139"
     CDS             8138..8398
                     /gene="mcbA"
                     /locus_tag="ACHXWD_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR7621981.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            8663..>8963
                     /locus_tag="ACHXWD_003140"
     CDS             8663..>8963
                     /locus_tag="ACHXWD_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR7621982.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         2134 a         2270 c         2247 g         2312 t
ORIGIN      
        1 ttccatacac cgctatccat ctaaatttaa atcacttttt cagagaactg cgtaagtatt
       61 acgcatgttt tccctgtcat tcatccagat tattcctaat caccagacta atgattccat
      121 caatcctggc gcattttagt caaaacgggg gaaaattttt tcaacaaatg ctcaaccagc
      181 attgggtata tccagtacac tccacgcttt acttaagtct agatatttgt gggagaaagg
      241 atgcctggtt cattacgtaa aatgccggtc tggttaccaa tagtcatatt gctcgttgcc
      301 atggcgtcta ttcagggtgg agcctcgtta gctaagtcac tttttcctct ggtgggcgca
      361 ccgggtgtca ctgcgctgcg tctggcatta ggaacgctga tcctcatcgc gttctttaag
      421 ccatggcgac tgcgctttgc caaagagcaa cggttaccgc tgttgtttta cggcgtttcg
      481 ctgggtggga tgaattatct tttttatctt tctattcaga cagtaccgct gggtattgcg
      541 gtggcgctgg agttcaccgg accactggcg gtggcgctgt tctcttctcg tcgcccggta
      601 gatttcgtct gggttgtgct ggcggttctt ggtctgtggt tcctgctacc gctggggcaa
      661 gacgtttccc atgtcgattt aaccggctgt gcgctggcac tgggggccgg ggcttgttgg
      721 gctatttaca ttttaagtgg gcaacgcgca ggagcggaac atggccctgc gacggtggca
      781 attggttcgt tgattgcagc gttaattttc gtgccaattg gagcgcttca ggctggtgaa
      841 gcactctggc actggtcggt tattccattg ggtctggctg tcgctattct ctcgaccgct
      901 ctgccttatt cgctggaaat gattgccctc acccgtttgc caacacggac atttggtacg
      961 ctgatgagca tggaaccggc gctggctgcc gtttccggga tgattttcct cggagaaaca
     1021 ctgacaccca tacagctgct ggcgctcggc gctatcatcg ccgcttcaat ggggtctacg
     1081 ctgacagtac gcaaagagag caaaataaaa gaattagaca ttaattaaat ttacatttct
     1141 gcatggttat gcataaccat gcagaatttc tcgctacttt tcctctacac cgtctttata
     1201 tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga taaataagaa
     1261 ttgttcttat caatatatct aactcattga atctttatta gttttgtttt tcacgcttgt
     1321 taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg gtgctatact
     1381 tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga gtaccgctaa
     1441 attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct ccgacagcga
     1501 gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg atctttcttt
     1561 gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg tacatgaaat
     1621 gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag aacgtgcagt
     1681 gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa ccccgctgaa
     1741 aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg ctgaccgtta
     1801 cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg acgacaccgc
     1861 agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta tcgagtctaa
     1921 catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa gcggcttcct
     1981 gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg catccatctg
     2041 ctatctccag ctgattaagt aaattttttg tatccacatc atcacacaat cgttacataa
     2101 agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc acaattttag
     2161 cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat aaatcgtgca
     2221 tcacgttttt gccgcatctc gaaaaatcaa ggagttgcaa aactggcacg attttttcat
     2281 atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat gctatgaagt
     2341 ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct tctcatgccg
     2401 cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa tttaaacagg
     2461 gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa gagctgaagc
     2521 tggattacga actgaagccg atggatttca gtgggatcat tccggcactg caaaccaaaa
     2581 acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa gcgatcgatt
     2641 tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac aataacgatg
     2701 tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt taagagcggt actggctccg
     2761 ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg aacatcgata
     2821 acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat acgccaaaca
     2881 ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt gactctctgg
     2941 aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt gacaaagtca
     3001 acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac aaaaaatggt
     3061 tcggtactga accgaaataa taacgctaca cctgtaaagc gcactggcag ttccctctcc
     3121 cctctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat taattaccct
     3181 gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac tggagtgcca
     3241 tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg atttcggtcc
     3301 tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc accttcggtg
     3361 gttggatagc caaccacgtc gcgctggtct ttattgaggt gatccgcggc acacctatcg
     3421 tcgtccaggt gatgtttatt tatttcgccc tgccgatggc gtttaacgac ttacgcatcg
     3481 acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac attgcggaaa
     3541 tcacgcgtgg tgcggtgctg tctattcaca aaggttttcg tgaagcagga ctggcgctcg
     3601 gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg cgtcgtatgc
     3661 tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg tttattgtga
     3721 tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac ttccgcgccc
     3781 ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg ctaagcttta
     3841 ttttgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa acgtctccaa
     3901 gcactttggc ccaacccagg tgcttcacaa tatcgatttg aacattgccc agggcgaagt
     3961 cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct gcatcaacaa
     4021 actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtta acgatccgaa
     4081 agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt tttacctctt
     4141 cccgcatctg acggcgctgg aaaacgtcat gtttggtccg ctacgcgtgc gtggcgcgaa
     4201 caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc tggcagaacg
     4261 tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg cgattgcccg
     4321 cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg ctcttgaccc
     4381 ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag ggatgacgat
     4441 ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc tgatctttat
     4501 cgacaaaggc cggattgcgg aagatggcga tccgcaggta ttgatcaaga acccgccgag
     4561 ccagcgcttg caggaatttt tgcagcacgt ctcttaactt ctctgcctcc ctttcacccg
     4621 aaagggaggc acaccagatt cctctcattt aaaatcgccc ctcccccagc atctatactt
     4681 atctttttgc tctgttttct cactggagga gtcatgcggt ggatcctgtt catcctcttc
     4741 tgcctgctgg gcgcacctgc ccacgcggta tccatacccg gcgttacaac cacaacgaca
     4801 acggactcaa cgactgaacc ggccccggaa ccggatatcg aacaaaaaaa agcggcctat
     4861 ggcgcactgg cggatgtgct ggataacgac acctcgcgta aagaattgat cgaccagttg
     4921 cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga agatcgtgcc gccgacgctg
     4981 gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca gccgccatta tggtgaagcc
     5041 ctttccgccc gcttcgggca actttatcgc aatatcaccg gctccccgca taagccattt
     5101 aatccacaaa ccttcagcaa tgcgctgagc catttttcaa tgttagcggt attagtgttt
     5161 ggtttttact ggctgattcg cctgtgcgca ctgccgctgt atcgcaaaat gggtcagtgg
     5221 gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc ttccggcgat gattatcggg
     5281 gcgtttatta tcgacctgct gttactggcc ctgacattat ttgtcggcca ggtattaagc
     5341 gacaacctga atgcaggcag tcgcaccatc gctttccaac aaagtttgtt tctcaacgcc
     5401 tttgccctga ttgaattttt caaagccgta ctacgcctga ttttttgccc aaacgtggcg
     5461 gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt actggagtcg tcgcctgagc
     5521 tggttaagca gtttgatagg ttatggcctg attgtggccg tgccgattat ctctaatcag
     5581 gtgaatgtac agataggtgc gctggcgaac gtcatcatta tgctgtgcat gaccgtctgg
     5641 gcgttgtacc tgatctttcg taataaaaaa gagattaccc agcatttgct caactttgcg
     5701 gagcattcgc tggccttttt cagcctgttt atccgcgcct ttgcgctggt gtggcactgg
     5761 ctggcaagcg cctattttat cgtgctgttt ttcttttcgt tgttcgatcc gggcaacagc
     5821 ctgaaattta tgatgggtgc aacggtgcgc agcctggcga ttattggtat cgcagcgttt
     5881 gtttccggta tgttttcccg ctggctggca aaaaccatca ctctctcgcc acatactcag
     5941 cgtaactatc cagagttgca aaaacggctg aatggctggc tttcggcggc gctgaaaacg
     6001 gcgcgtattc tgaccgtctg cgtggcggta atgttgctgt tgagcgcatg gggattgttc
     6061 gatttctgga actggctgca aaacggcgcg gggcagaaaa ccgtagatat cctgatccgt
     6121 atcgcactta ttcttttttt ctcggcggtt ggctggacgg tgctcgccag tttgatcgaa
     6181 aaccggctgg cttcggatat tcatggccgc ccgctaccca gcgcccgtac gcgtaccctg
     6241 ctgacgctgt tccgtaacgc gctggcggtg attatcagta ccatcaccat catgattgtg
     6301 ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag gtgccggggc attaggtctg
     6361 gctatctcgt ttggttcgca aacgctggtg aaagatatca ttaccggggt gtttattcag
     6421 tttgaaaacg gcatgaacac tggagatttg gtgactatcg ggccgttgac cggcacagtg
     6481 gaacggatgt cgattcgctc cgtgggcgtg cgccaggata ccggggcgta tcacatcatt
     6541 ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg gtattggttc ggtagtggca
     6601 aactacgatg ttgatcgcca tgaagatgct gataaagcca atcaggcact gaaagatgcg
     6661 gtagcggaat taatggaaaa cgaagaaatt cgcgggctga ttattggtga accgaatttt
     6721 gccgggattg tcggcttaag caataccgcg tttacactgc gtgtttcgtt caccacgctg
     6781 ccactcaaac agtggacggt acgctttgcc ctcgacagcc aggtgaaaaa acatttcgac
     6841 ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc tgcctactcc gggcgcgacc
     6901 ccggctgaac cgttaccgcc gggggagcca acgctttaac gctggcgatt gacaaaacgg
     6961 cggcgctgct cgtcgttcat aaaggtccag gcaataaagc gactctgctt ttgcccctgg
     7021 gccatctctt tttttaccac cttcaccgcg cccacgtcgg tcagagcacg atacaacggc
     7081 ggtaagtttt caccacgaga taccagcgag gtaaaccaca tcacctgctt cgcgaagcct
     7141 ttgctctctt caatcatctt tttgataaag gcgacttcac caccttcaca ccacaactcc
     7201 tgttgctgac caccaaagtt cagtgcatcg tctttgttca gccccaggtt acggcgttta
     7261 cgctcactac ctgcccgcgc tgcagcggcg gaatcgtgga atggcgggtt acacaaggtc
     7321 gcgtcgtatt gctcgttttt atggatgatg ccgttaaaaa ttgccccact ctctttttgc
     7381 cgacgcagac gaatggcgcg gttaagcccc ggattagcac tgataatcgc ctgcgcactg
     7441 cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc agccatattc atgtacgcca
     7501 atcagcggat aaatacagtt cgcaccaacg ccgatatcca gaatgctggc attcgctgga
     7561 attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat gaatataatc cgcccggccc
     7621 ggtactggtg ggcagagaaa accgtcgggg atatcccagt tcgctacggc gtaaaaatgg
     7681 gccagcaacg ccttattgag cgccttcacc gccagcggat tggcaaagtc tacgctttgc
     7741 tccccggcgg gtgtaagcgt gaggaattgc ctgagttcag gattgacctg acaaagcgtg
     7801 gcgagatcgt agcggctgtg atgacggttg cgcggatgca accccggttt ctgggcggac
     7861 atagcattct cctttaagca gcggcgtaag atacccgcat aacgcgacag ggtaaatatc
     7921 tggctgcgtt tatcattccc caaaacctta acatggctat ggtttatggc aattcatcat
     7981 aaaaatcagc attatgcatg tcattaattt gtcttatttt gtaaaaatat atcgttcaaa
     8041 aaataagcac atctctgtca attttttgcg tcatttgcat cgcgcaggcg acctacactt
     8101 atcaggcact acccgttgta tttatgagga tgtcattatg aaaaagtgcc tcacactact
     8161 gattgccacc gtcctgagcg gaatctctct cacggcttat gccgcgcaac cgatgagtaa
     8221 cctggacagc ggtcagctac ggcccgccgg taccgtttcg gcaactggcg catcaaacct
     8281 aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa caaggcgcga aaggttatgt
     8341 cattaattcc gcaggcggaa atgaccagat gttcggtact gcaaccatct acaaataacc
     8401 ccaccaacgt aactgcattc gcctctggat gcagttttcg cctgttttgt aatcaaaaaa
     8461 catcaaatat gaactcaatg taaataaatg tatttctttt tcgcgcaatg ggtgatagaa
     8521 aatcgcgcca agtgataatg cttatcaaaa ttattatcac ttgcatgaac actatcacgg
     8581 gattaacagt ggcatcgcat ccgcagagat gctttctcgt ggcagtgaaa atttcaacat
     8641 ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat ttccctgcca gacaatttca
     8701 ttcgctcacg ttctttgccg gtctttgtat tggcatcacg cctgtggctc aggcactcgc
     8761 cgccgaaggg caaactaacg cggatgacac gctggttgtc gaagcatcaa cgccttcgct
     8821 ttatgcgcca caacaatctg ccgatccgaa attctcgcgt ccggtagcgg atactacccg
     8881 cacgatgacg gtgatttctg aacaagtgat taaagatcag ggcgcaacca accttaccga
     8941 cgcgctcaaa aacgcccccg gcg
//
LOCUS       DAZSBF010000166         8837 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZSBF010000166 DAZSBF010000000
VERSION     DAZSBF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.169"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            86..1810
                     /gene="iucA"
                     /locus_tag="ACHXWD_003141"
     CDS             86..1810
                     /gene="iucA"
                     /locus_tag="ACHXWD_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR7621983.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1811..2758
                     /gene="iucB"
                     /locus_tag="ACHXWD_003142"
     CDS             1811..2758
                     /gene="iucB"
                     /locus_tag="ACHXWD_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR7621984.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2758..4500
                     /gene="iucC"
                     /locus_tag="ACHXWD_003143"
     CDS             2758..4500
                     /gene="iucC"
                     /locus_tag="ACHXWD_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR7621985.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4497..5834
                     /gene="iucD"
                     /locus_tag="ACHXWD_003144"
     CDS             4497..5834
                     /gene="iucD"
                     /locus_tag="ACHXWD_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR7621986.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            5840..8035
                     /gene="iutA"
                     /locus_tag="ACHXWD_003145"
     CDS             5840..8035
                     /gene="iutA"
                     /locus_tag="ACHXWD_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR7621987.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDFLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(8586..8729)
                     /locus_tag="ACHXWD_003146"
     CDS             complement(8586..8729)
                     /locus_tag="ACHXWD_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7621988.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2118 a         2244 c         2343 g         2132 t
ORIGIN      
        1 tatctgacaa taacatttat tgttgataat gagaatcatt atcaacataa ttgttattat
       61 tttactgtgt gggagctgtt tgactatgac cctgccctct gaaaaaccag ccacagatgt
      121 ggctgcgcag tgcttcctga atgcactgat tcgtgaaacc acagactgga aactgacaga
      181 atacccgcca gacgaattgc tcatcccgct ggatgagcag aaatcgctcc atttcagagt
      241 ggcttatttc tccccaaccc agcatcaccg ctttgcattt cctgcacgtc tggtcacggc
      301 atcaggcagt tatcctgtcg actttaccac tctctcccgg cttattattg ataagctacg
      361 ccatcaactg tttctgcccg ttcccctctg cgaaactttc caccagcgcg tgctggaaag
      421 ccatgtccat acgcaacagg caattgatgc ccgtcatgac tgggccgctc tgcgtgaaaa
      481 agcgttgaat tttggcgagg ctgagcaggc gctgctgaca ggacacgctt tccaccctgc
      541 gcctaagtct catgaaccgt ttaaccgacg ggaggctgaa cgctacctgc ctgatatggc
      601 accccacttc ccactgcggt ggttttcggt ggataaaacg caaatcgccg gtgaaagttt
      661 gcatctcaac cttcaacagc ggctgacgcg atttgccgca gagaatgcgc ctcagttact
      721 caacgaatta agtgacaacc aatggctgtt cccgttgcac ccgtggcagg gggaatatct
      781 tttgcagcag gggtggtgcc aggcacttgt tgctaaaggg ctgattaaag acttaggtga
      841 ggccggcacg tcgtggctgc cgaccacctc ttcccgttcc ctctactgtg ccaccagccg
      901 cgatatgatc aagttctccc tgagcgtacg gctgaccaac tccatccgta ctctgtccgt
      961 gaaagaagtg aagcgtggga tgcgcctggc acgcctggct caaaccgacg gctggcagat
     1021 gctacaggtc cgcttcccga ctttccgggt aatgcaggag gatggctggg ccgggctgct
     1081 cgatcttaac ggcaacatca tgcaggaaag tctgtttgcc ctgcgtgaaa atctgctggt
     1141 ggatcagccg aaaagccaga ctaacgtact ggtctccctg actcaggccg cacctgatgg
     1201 cggtgattcg ctgctggttt cggcggtaaa acgcctgagc gatcgcctcg gtatcactgt
     1261 gcaacaggcc gcccatgcat gggtcgatgc atactgtcag caagtgctaa agccgctgtt
     1321 tacggctgaa gcggattacg gcctggtgct gctggcgcat cagcaaaata ttcttgtcca
     1381 gatgcttggg gatctgccgg tcggatttat ttaccgtgac tgtcagggca gcgcttttat
     1441 gcctcacgcg acagactggc tcgattctat tggcgaggcg caggcggaaa atattttcac
     1501 ccatgagcag ttgctgcgct atttccctta ttacctgctg gttaactcca cttttgctgt
     1561 gaccgctgcg ctgggggctg ccgggctgga cagcgaatcg aatctgatgg ctcgtgtacg
     1621 agcatcgctg gctgaagtgc gtgatcaggt gactcataaa acctgtctca actacgtgct
     1681 ggaaagtccg tactggaacg taaaaggtaa ctttttctgt tatctgaacg atcataacga
     1741 gaacaccatt gttgaccctt cggtgatcta tttcgatttc gctaacccgc tgcaggctca
     1801 ggaggtctga atgtctgagg caaacattat tcacagcaga tatggactgc gctgtgaaaa
     1861 actcgacaag cccctgaatc ttggctgggg actggacaat agcgcggtgt tgcactgtcc
     1921 cggggagctg ccgacagggt ggctgtgcga tgcgctggat cagatattta tcgccgcacc
     1981 acaactgtca gcagtggctc tcccctgggc cgaatggcgt gaggagccac aggcgctgac
     2041 gctttttggg caggtaaaaa gcgacattat ccatcgtact gccttctggc agttaccgtt
     2101 atggttgagt tcaccggcaa accgggcctc cggtgaaatg gtttttgatg cagagcgtga
     2161 gatttatttc ccgcagcgcc caccccgtcc acagggtgag gtttaccgcc gttacgatcc
     2221 acgtattcgc aggatgctga gtttccgcat tgccgatccc gtttctgatg cagaacgttt
     2281 cacccgctgg atgaatgatc cgcgcgttga gtatttctgg gagcaaagtg gctcactgga
     2341 ggtacagacc gcctatctgg agcgccagct caccggtaaa catgcattcc cgctgattgg
     2401 gtgcttcgac gatcggccat tcagctattt cgaaatctac tgggcggcgg aagaccgcat
     2461 tggtcgtcac tattcgtggc agccctttga ccgtggcctg catctgctgg ttggtgaaca
     2521 gcaatggcgc ggggcccact atgtgcaaag ctggctgcgc gggctgacac attacctgct
     2581 gctggatgag ccccgtacgc agcgcacagt actggagcca cgcaccgata accagcgcct
     2641 gttccgccat cttgagcctg cgggataccg gacaattaaa gagttcgact tcccacataa
     2701 gcgctcgcgc atggtgatgg cggatcgtca tcacttcttc acggaggtcg gtctgtaatg
     2761 aatcacaagg actgggattt tgttaaccgt cggctggtgg caaaaatgtt gtctgagatg
     2821 gagtatgagc aggttttcca cgcggaatct cagggcgatg accactactg cattaacctg
     2881 ccgggagcac aatggcgctt catcgctgaa cgtggtatct ggggctggct gtggattgat
     2941 gcccaaacgt tgcgctgcac ggacgaacca gtactggcgc agacgctgct gatgcagcta
     3001 aagccggtgc tgtcaatgag tgatgccact gttgcagagc atatgcagga tttgtatgcc
     3061 acgctactgg gcgacctgca actgctgaag gcccgtcgcg gactgagtgc cagtgacctg
     3121 attaatcttg atgccgatcg tctgcaatgc ctgctgagcg gtcatcctaa attcgttttt
     3181 aataaaggtc gccgtggctg gggcaaagag gcgctggaac gatatgcgcc agagtatacc
     3241 aacaccttca gactgcactg gctggcggta aaacgtgaac atatgatctg gcgctgcgat
     3301 aacgatctgg atattcagca gttgttgact gccgcaatgg atccgcagga gtttacacgc
     3361 ttcagtcagg tctggcagga aaacgggctg gatcacaact ggctaccgct gccggtacat
     3421 ccgtggcagt ggcagcaaaa aatcgccaca gacttcatcg ctgattttgt cgaaggcagg
     3481 atggtgtctc tcggcgagtt tggcgaccag tggctcgccc agcagtcgct gcgtaccctg
     3541 accaacgcca gccgccgggg agggctggat atcaagctgc cgctgaccat ctacaacacc
     3601 tcatgctacc ggggaatccc tggcagatat atcgctgccg gaccactggc ttcacgctgg
     3661 ctgcaacagg tttttgcgac cgacgccacc ctggtgcaaa gcggcgcagt gatccttggt
     3721 gaaccggctg caggctatgt gtcccatgaa ggctatgccg cgcttgcccg ggctccctat
     3781 cgctaccagg aaatgcttgg tgttatctgg cgggagaatc cgtgccgctg gctgaaaccg
     3841 gatgaaagtc ctgttctgat ggcaacactg atggagtgcg acgaaaacaa tcagccgctg
     3901 gcaggcgcat atatcgaccg ctccggactg gacgctgaaa cctggcttac gcaattgttc
     3961 cgggtggttg tggttccgct gtatcacctg ctttgccgct acggtgtcgc gcttattgca
     4021 cacggacaaa atataacgct cgccatgaaa gagggggtac cacagcgtgt tctgctgaaa
     4081 gacttccagg gcgatatgcg gctggtgaaa gaagcgtttc ccgaaatgga ctctttgcct
     4141 caggaggtgc gtgatgccac atcccgtctg agtgcggact acttaatcca tgatttgcag
     4201 acgggtcact tcgtgacagt actgcgtttt atttcgccac tgatggttcg tcttggcgta
     4261 cctgaaaggc gattttatca actgctggca gcagtgttga gtgattacat gaacaaacat
     4321 ccacaaatgg cagagcgttt tgcgcttttc tcactcttca ggccacaaat cattcgcgtg
     4381 gtgctgaacc cggtaaaact gacctggccg gacctggatg gcggcagtcg catgctgccg
     4441 aattaccttg agaatctgca aaatccgctg tggctggtaa ctcaggaata tgaatcatga
     4501 aaaaaagtgt cgattttatt ggtgtcggga cagggccatt taatctcagc attgctgcat
     4561 tgtcacatca gatcgaagaa ctgaactgtc tcttctttga cgaacatcct catttttcct
     4621 ggcatccggg tatgctggta ccggattgtc atatgcagac cgtctttctg aaagatctgg
     4681 tcagtgcagt tgcacctaca aatccctaca gctttgttaa ctatctggtg aagcacaaaa
     4741 agttctatcg cttccttaca agcaggctac gtacagtatc ccgtgaagag ttttctgact
     4801 atctccgctg ggctgctgaa gatatgaata acctgtattt cagtcatacc gttgaaaata
     4861 ttgatttcga caaaaaaagt cgattgtttc tggttcagac cagtcgggga gaatattttg
     4921 cccgcaatat ctgccttggt acaggaaaac aaccttattt accaccctgt gtgaagcatg
     4981 tgacacaatc ctgtttccat gccagtgaaa tgaatcttcg tcggccggac ctgagtggaa
     5041 aacggataac cgtggttggt ggaggacaga gtggtgcaga cctgttcctt aatgcattac
     5101 gcggggaatg gggagaagcg gcggaaataa actgggtctc acggcgtaat aattttaacg
     5161 cactggatga ggctgctttt gctgatgagt attttacacc tgaatatatt tcaggcttct
     5221 ccggactgaa ggaagatatt cgccatcagt tactggatga gcagaaaatg acatcggatg
     5281 gcatcactgc cgattcttta ctgaccattt atcgtgagtt gtaccaccgt tttgaagttc
     5341 tgagaaaacc aagaaatatc cgtctgctac ccagtcgctc ggtaacaacc ctggaaagta
     5401 gtggtccggg ctggaggtta ttgatggaac atcatctgga tcagggcaga gagagcctgg
     5461 aaagtgatgt ggtgattttc gccacaggtt accgttctgc attgccacaa atacttccct
     5521 cactgatgcc cttgatcacc atgcacgata agaacacctt taaagtgcgt gatgacttca
     5581 ctctggaatg gagtggcccg aaagaaaata acatctttgc ggtcaacgcc agcatgcaaa
     5641 ctcatggcat cgccgaaccc cagctcagcc tgatggcctg gcgatctgca cgtattctta
     5701 atcgcgtact ggggcgtgat ttattcgatc tcagtatgcc gcccgcactg attcagtggc
     5761 gcagcggcag ccggaaaaaa ccgcagccgg aggctgcttc tttaactcac tatacagcaa
     5821 atattcagga ataacgatga tgcgcaaaaa gtatatgccc cgggctcttg gtccgctgct
     5881 tcttgtcgtg ctgtcaccag ctgtcgccca gcaaaacgat gataatgaga tcatagtgtc
     5941 tgccagccgc agcaatcgaa ctgtagcgga gatggcgcaa accacctggg ttatcgaaaa
     6001 tgccgaactg gagcagcaga ttcagggcgg taaagagctg aaagacgcac tggctcagtt
     6061 aatccccggc cttgatgtca gcagccagag tcgaaccaac tacggtatga acatgcgtgg
     6121 ccgcccgctg gttgtcctga ttgacggtgt gcgcctcaac tcttcacgtt ccgacagccg
     6181 acaactggac tctgtcgatc cttttaatat cgaccatatt gaagtgatct ccggcgcgac
     6241 ggccctgtac ggtggcggga gtaccggagg gttgatcaac atcgtgacca aaaaaggtca
     6301 gccggaaacc atgatggagt ttgaggctgg cacaaaaagt ggctttaaca gcagtaaaga
     6361 tcacgatgag cgcattgccg gtgctgtctc cggcggaaat gaccatatct ccggacgtct
     6421 ttccgtggca tatcagaaat ttggcggctg gtttgacggt aacggcgatg ccaccctgct
     6481 tgataacacc cagaccggcc tgcagcactc caatcggctg gacatcatgg gaaccggtac
     6541 gctgaacatc gatgaatccc ggcagcttca actgataacg cagtactata aaagtcaggg
     6601 agacgacaat tacgggctta atctcgggaa aggcttttcc gccatcagcg ggagcagcac
     6661 accatacgtc agtaaggggc tgaattctga ccgcattccc ggcactgagc ggcatttgat
     6721 cagcctgcag tactctgaca gtgatttcct gggacaggaa ctggtcggtc aggtttacta
     6781 ccgcgatgag tcgttgcggt actacccgtt cccgacggta aatgcgaata aacaggcgac
     6841 agctttctcc tcgtcacagc aggataccga ccagtacggc atgaaactga ctctgaacag
     6901 ccaactgatg gacggctggc aaatcacctg ggggctggat gctgagcatg agcgctttac
     6961 ctccaaccag atgttcttcg atctggctca ggcaagtgct tccggagggc tgaacaacca
     7021 taagatttac accaccgggc gctatccgtc atatgacatc accaatctgg cggccttcct
     7081 gcaatccagc tatgacatta atgatatttt taccgttagc ggtggcgtac gctatcagta
     7141 tactgagaac agggtagatg atttcatcga ctacacgcag caacagaaga ttgctgccgg
     7201 gaaggcgata tctgccgacg ccattcctgg tggttcggta gattacgata actttctgtt
     7261 caatgctggt ctgctgatgc acatcaccga acgtcagcag gcatggttca atttttccca
     7321 gggggtggca ttgccggatc cggggaaata ttatggtcgc ggcatctatg gtgcagcagt
     7381 gaacggccat ctttccctga caaagagcgt gaacgtcagc gacagtaagc tggaaggcgt
     7441 gaaagtcgat tcttatgaac tgggctggcg ctttaccggt gacaacctgc ggactcaaat
     7501 cgcggcatat tactcgcttt ccaataagag cgtggaaagg aataaagatc tgaccatcag
     7561 tgtgaaggac gacaggcgcc gtatttacgg cgtggaaggt gcggtggact tcctgatccc
     7621 ggatactgac tggagtaccg gtgtgaactt caatgtgctg aaaaccgagt cgaaagtgaa
     7681 cggtcaatgg caaaaatatg acgtgaagga atcaagtcca tcgaaagcga cagcttacat
     7741 taactgggcg ccggaaccgt ggagtctgcg tgtacagagc accacttctt tcgacgtaag
     7801 cgatgcagag ggtaacgata ttaatggtta cactaccgtc gattttatca gtagttggca
     7861 gcttccggtg ggaacactca gcttcagcgt tgagaacctc ttcgaccgtg actataccac
     7921 tgtctgggga cagcgtgcac ctctgtacta ctgcccgggt tacggccctg cttcactgta
     7981 cgactacaaa ggccggggcc gaacctttgg tctgaactac tcagtgctgt tctgacgggg
     8041 atgcttttgc aacaagggta tgctgtaaac aacatggtcc ttggtggcca tgttgtttac
     8101 agccagtata atatacagcc ttaacaagat attccgtctc cggaatggac tgttcaccac
     8161 tgaataacat tatttctgtt ttatggatct ttgactgaaa tttatcgcat atcctcattt
     8221 cattgtccgt ctgaaaacta accttacaag taagcgtcgt ctcagcaccg tctggcagat
     8281 cctgaaattc ctgagaggat gatctgcgcc caatatcctg agccggaact ttgctgcaat
     8341 gaaggccaac gaaaaatggg ggagtatgtt atagagtact tgctacaggg taaaaagagg
     8401 tacctgtcgc tggttctccg ttcagtttaa ccgggagatt ttctcctgcg cctgtcataa
     8461 agtccggtaa tggagattgt taatacgatg gtgcacgatg cattgtcggt gcccggcccg
     8521 gaggatgccc cgtaactaca tacggatcag ggttggcaat atcgaatggc aggctatagg
     8581 caaagttaaa gccccatgga gtagcacaaa atatgccaca caaaggaaac ggtctgaata
     8641 acgcagtgat ggagaacttc ttcggcacac tgcaaaatcg catcgtggtc gatcgctaat
     8701 tcgccgtgaa tcactgaacg atgttccatg cgaacagata ttcgatcata tcgaaatatt
     8761 aaacattacc cctccccgca ttgggcaaaa aaaaaacaaa aaacgatcaa ttagtattga
     8821 ttcacataat ctctcat
//
LOCUS       DAZSBF010000167         8795 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZSBF010000167 DAZSBF010000000
VERSION     DAZSBF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.170"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            328..894
                     /gene="mepS"
                     /locus_tag="ACHXWD_003147"
     CDS             328..894
                     /gene="mepS"
                     /locus_tag="ACHXWD_003147"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR7621989.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            1075..2631
                     /locus_tag="ACHXWD_003148"
     CDS             1075..2631
                     /locus_tag="ACHXWD_003148"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR7621990.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            2713..4527
                     /locus_tag="ACHXWD_003149"
     CDS             2713..4527
                     /locus_tag="ACHXWD_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR7621991.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            4528..5622
                     /locus_tag="ACHXWD_003150"
     CDS             4528..5622
                     /locus_tag="ACHXWD_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR7621992.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            5622..6647
                     /locus_tag="ACHXWD_003151"
     CDS             5622..6647
                     /locus_tag="ACHXWD_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7621993.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            6649..8238
                     /gene="yejF"
                     /locus_tag="ACHXWD_003152"
     CDS             6649..8238
                     /gene="yejF"
                     /locus_tag="ACHXWD_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR7621994.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(8242..8586)
                     /locus_tag="ACHXWD_003153"
     CDS             complement(8242..8586)
                     /locus_tag="ACHXWD_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR7621995.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT         2086 a         2196 c         2186 g         2327 t
ORIGIN      
        1 aaaaatcatt ctctaagtaa atgaatggat tgcatgcgtt tcactcaatc gtactttaat
       61 tgaccaatcc cgcttattaa ctttctgtat cactttttct tataaaaaat catgtaaaac
      121 cgctcgccaa gaccgcacca atcgggtaat ctagaactcg ttttgcctcg gcggtagatt
      181 atcctcacag catataattt tgtgcgttag tccacagatt tggccttaag gaattgtttc
      241 aacatgccca ggtaattagt ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt
      301 taaggacttc aagggaaaac aaacaacatg gtcaaatctc aaccgatttt gagatatatc
      361 ttgcgcggga tccccgcgat tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac
      421 accgcaaaga atatgcatcc tgagacacgt gcagtgggta gtgaaacatc atcactgcaa
      481 gcttctcagg atgaatttga aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg
      541 gatcagtatg ctgactggaa aggcgtacgt tatcgtctgg gcggcagcac taaaaaaggt
      601 atcgattgtt ctggttttgt acaacgtaca ttccgtgagc aatttggctt agaacttccg
      661 cgttcgactt acgaacagca ggaaatgggt aaatctgttt cccgcagtaa tttgcgtacg
      721 ggtgatttag ttctgttccg tgccggttca acgggacgcc atgtcggtat ttatatcggc
      781 aacaatcagt ttgtccatgc ttccaccagc agtggtgtta ttatttccag catgaatgaa
      841 ccgtactgga agaagcgtta caacgaagca cgccgggttc tcagccgcag ctaataaacc
      901 gtttggatgc aatcccttgg ctatcctgac gagttaactg aaagcactgc ttaggcagtg
      961 cttttttgtt ttcattcatc agagaaaatg atgtttccgc gtcttgatcc aggctatagt
     1021 ccggtcattg ttatctttta aatgttgtcg taatttcagg aaattaacgg aatcatgttc
     1081 atacgcgctc ccaattctgg acgtaagctc ctgcttacct gcattgtagc aggcgtgatg
     1141 attgcgatac tggttagctg ccttcagttt ttagtggcct ggcataagca cgaagtcaaa
     1201 tacgacacac tgattatcga cgtacaaaag tatctcgata cctattttgc cgacctgaaa
     1261 tccactactg accggcttca gccgctgacc ttagatacct gccagcaggc taaccccgaa
     1321 ctgaccgctc gcgcggcgtt tagcatgaat gtccgtacgt ttgtgctggt gaaagataaa
     1381 aaaacattct gttcatctgc gactggtgag atggacattc cactcaatga attgattccg
     1441 gcgctcaaca ttaataaaaa cgtcgatatg gcgatcttac ccggcacgcc gatggtgccg
     1501 aacaaacccg caatcgtcat ctggtatcgc aaccctttgc tgaaaaatag cggtgtgttt
     1561 gccgctctga atctcaacct gacgccttct ctcttttata gttcacggca ggaagattac
     1621 gatggcgtcg ccctcattat tggcaatact gcgctatcta ccttttcttc acgtttgatg
     1681 aacgttaatg aattaaccga catgccggtc cgtgaaacta aaattgcggg cattcctctg
     1741 accgttcggc tttatgcgga tgactggaca tggaacgatg tgtggtacgc atttttactg
     1801 ggcggcatga gtggaactgt cgttggcctg ctctgctatt acctgatgag cgtacgtatg
     1861 cgccccggca gagaaatcat gaccgccatc aagcgcgaac aattttacgt ggcgtatcaa
     1921 ccggtggtgg atacacaagc tttgcgagta acgggcctgg aagtactgct acgctggcgg
     1981 catcctgtcg cgggagaaat tcccccggat gccttcatta actttgccga atcgcaaaag
     2041 atgattgtgc cgctgactca gcacctgttt gagttaattg cccgcgatgc cgcagaatta
     2101 gaaaaagtgc tgccagtagg cgtcaaattt ggtattaaca ttgcgccgga ccatctgcac
     2161 agcgaaagct ttaaagcaga tatccagaaa ctgctcactt ccctgcccgc acaccatttc
     2221 cagattgtgc tggaaattac cgagcgcgat atgctgaaag agcaagaagc cacacaactc
     2281 ttcgcctggc tgcactcggt cggcgtagaa attgctattg atgacttcgg caccgggcac
     2341 agcgcgctta tctatcttga gcgttttacg ctcgattatc tgaaaattga ccgtggattt
     2401 atcaacgcca tcggtacgga aacgatcact tcacccgtac ttgacgcggt gctgacgctg
     2461 gcgaaacgcc tcaatatgct gacggttgct gaaggggtcg aaacgccgga acaggcgcga
     2521 tggctaagcg aacgcggcgt taatttcatg caaggctact ggattagtcg ccctttaccg
     2581 ctggacgatt ttgtccgctg gctgaagaaa ccgtatacgc cgcagtggta aggtgtgctt
     2641 acgtccctta ttattcatag tgaaagcatg ccggattgcg gctaatgatg agtaaaagga
     2701 aatccattgc agatgattgt gcgcatactg ctgctgttta tcgctctgtt cacctttggt
     2761 gtgcaggcgc aggctatcaa ggaaagctat gcctttgccg tgctgggcga accccggtac
     2821 gcgtttaatt tcaaccattt tgattatgtg aaccccgccg cgccaaaagg tgggcagata
     2881 acgttgtcag ccctcggcac cttcgataat ttcaaccgct atgcactgcg cggcaacccg
     2941 ggcgcacgca ccgagcagct gtacgacacg ctatttacga cttccgatga cgaaccaggc
     3001 agttattacc cgctgattgc tgaaagcgca cgctatgctg acgattattc ctgggtggag
     3061 gtcgctatta atccgcgcgc ccgttttcat gatggttcgc ccattactgc ccgcgatgta
     3121 gagtttactt ttcaaaaatt tatgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa
     3181 ggcaccaccg tcaaagccat tgcaccgtta accgtgcgca ttgagttagc taaacccagc
     3241 aaagaagata tgctgagtct gttttcgctg ccgatatttc cagaaaagta ctggaaggat
     3301 cacaaactta gcgacccgct cgccacgcct ccgcttgcca gtggtccgta ccgcgttacg
     3361 tcctggaaaa tggggcaaaa tattgtctat tcccgcgtga aagattactg ggcagcaaac
     3421 ttaccggtaa accgtggacg ctggaatttc gacaccattc gctacgatta ttacctcgat
     3481 gataatgtcg cctttgaagc gtttaaagca ggtgcctttg atttgcgtat ggaaaacgac
     3541 gctaaaaact gggccacgcg ttataccggt aaaaatttcg ataaaaaata catcatcaaa
     3601 gatgagcaaa agaacgaatc agcccaggat acgcgctggc tggcgtttaa tatccaacgt
     3661 ccggtattca gcgatcgccg ggtccgggaa gcaatcactc tcgcctttga ctttgaatgg
     3721 atgaacaagg cgttgtttta caacgcctgg agtcgcacga acagttattt tcagaatacc
     3781 gaatacgcgg ccagaaatta ccccgacgcc gcggagctgg tgcttctggc accaatgaaa
     3841 aaagatctac cgccagaagt cttcacacaa atctaccagc cgccggtatc taaaggcgat
     3901 ggctacgatc gtgacaacct gttaaaagcc gacaaacttc tcaacgaaac gggctgggtg
     3961 ctgaagggtc agcaacgcgt taatgccaca acgggtcagc cgctcagctt tgaattattg
     4021 cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc agcacagcct gcaacggctg
     4081 ggtatcaata tggacattcg caaggtggat aactcgcaaa tcactaaccg catgcgcagt
     4141 cgcgactatg acatgatgcc gcgcctatgg cgggcgatgc cgtggcccag ttccgattta
     4201 cagatttcct ggtcatcgga atatatcaat tccacttata atgcccccgg cgtgcaaagc
     4261 ccggttatcg actcgctgat caaccaaatt attgccgcgc agggaaataa agaaaaatta
     4321 ctgccgttgg ggcgagcact ggatcgcgta ttaacgtgga attattacat gctgccaatg
     4381 tggtacatgg cggaagaccg tctcgcctgg tgggataaat tctcccaacc cgctgtacgc
     4441 cctgtttaca gcctgggtat cgatacctgg tggtatgacg ttaataaagc ggccaaactg
     4501 ccgtcagcca gacaacaggg agagtagatg ggcgcttacc tgattcgccg tctgttgctg
     4561 gtgatcccaa cgttatgggc tattatcact atcaactttt tcatcgtgca aattgcgcct
     4621 ggcggtccgg tcgatcaggc catcgccgcc attgagtttg gtaatgccgg agtattaccc
     4681 ggcgcaggcg gtgaaggtgt tcgtgccagc catgcgcaaa cgggtgtcgg caatatcagc
     4741 gacagtaatt accgtggcgg acgcggatta gacccagaag tgatcgctga gatcactcat
     4801 cgctacggtt tcgataagcc gatccacgaa cgttacttca aaatgctctg ggactacatc
     4861 cgctttgatt ttggtgatag cctgtttcgc agcgcctcgg tgctgacgct gattaaagac
     4921 agtctgccgg tttccatcac cctcggattg tggagcacgc tgattatcta tctggtgtcg
     4981 attccgttag gcattcgcaa agctgtttat aatgggagtc gctttgacgt ctggagtagc
     5041 gcatttatca tcatcggcta cgccattccg gcctttttgt ttgccatcct gctgattgtc
     5101 ttcttcgcgg gcggcagcta tttcgacctg ttccctctac gcggcctggt ttccgctaac
     5161 tttgattcgc tgccgtggta tcagaaaatc accgattatc tgtggcatat cacgctaccg
     5221 gtgctagcga cagtgattgg tggctttgcg gcgctgacca tgctgaccaa aaactcattc
     5281 cttgatgaag tgcgcaagca atacgtggtg accgcgcgtg cgaaaggggt aagtgagaaa
     5341 aacattctct ggaaacatgt gttccgcaac gccatgctgc tggtgattgc cggttttccg
     5401 gcgacgttta tcagcatgtt ttttaccggc tcgctgctga ttgaggtgat gttttcactc
     5461 aatggtcttg gcttactggg ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt
     5521 ggtaccttgt atattttcac cctgattggc ctgctgctga atattgtcag tgatatcagc
     5581 tatacgctgg ttgatccgcg tatagatttt gagggacgtt aatgtcgcga ctcagccccg
     5641 tcaatcaggc ccgttgggcg cgttttcgtc ataaccgtcg cggctactgg tcgttatgga
     5701 ttttcctcgt tttgtttggt ttgagtttgt gttctgaact tatcgctaac gataaaccgt
     5761 tgctggtgcg ttatgacggc agttggtatt tcccgttgtt gaaaaactac agcgaaagcg
     5821 attttggcgg cccgctggca agtcaggctg attatcagga cccgtggctg aaacaacggc
     5881 tggaaaataa cggctgggta ctgtgggcac cgattcgctt tggtgctacc agtatcaact
     5941 ttgctaccga taagcccttc ccttctccac cctcccggca aaactggctg ggaacggatg
     6001 ccaacggcgg cgatgtgctg gcacgcattc tctatggcac gcggatctcg gttctgtttg
     6061 gcctgatgct gactctctgt tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct
     6121 attacggcgg taaagtcgat ctctggggac aacgctttat tgaagtatgg tcggggatgc
     6181 cgacgctgtt tttgattatt ttactttcca gcgtcgtaca gcctaacttc tggtggctgc
     6241 tggcaattac tgtcttgttt ggctggatga gtctggtcgg cgtggtgcgg gcggagtttt
     6301 tacgtactcg taatttcgac tacattcgtg cggcacaggc gcttggcgtc agcgatcgca
     6361 gtatcatcct gcgtcatatg ttgcctaatg ccatggtcgc gaccctcacc tttttaccgt
     6421 ttattttatg tagttcgata accaccctga cctcgctcga tttcctcggc ttcggtctac
     6481 cgctcggttc accgtcactc ggtgaactgc tgttacaagg gaaaaataac cttcaggccc
     6541 cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt attgtctttg ctgatcttta
     6601 ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc ggtgtagcat gacgcaaact
     6661 ctgttagcga ttgaaaattt gtcggtgggt tttcgccatc agcaaaccgt acgtacagta
     6721 gtcaatgatg tttcactaca gattgaggct ggcgaaacgc tggcgctggt gggtgagtca
     6781 ggttcaggca aaagcgttac cgcgctgtca attttacgcc tgctcccttc cccgccggtt
     6841 gaatatctct ccggcgatat tcgttttcat ggcgaatcgc tgcttcatgc cagcgatcaa
     6901 acgttgcgcg gtgtacgcgg taataagatc gccatgattt ttcaggaacc gatggtgtcg
     6961 ttaaatccat tgcataccct ggaaaaacag ctttatgaag tgctttcact ccaccgcggg
     7021 atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc ttgatcgcgt cggtatccgc
     7081 caggcggcaa aacggctgac agattatccg catcagctct ccggcggcga acgccagcgg
     7141 gtgatgattg cgatggcgct gttaacgcga ccggaattat taattgccga tgaaccgacc
     7201 accgcactgg acgtctctgt ccaggcgcag attttacagc tgttgcgcga actgcaaggc
     7261 gagctgaata tgggcatgct gtttattact cataacctca gcattgtcag aaaactggcc
     7321 caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc aaaataacgc cgctacgcta
     7381 tttgcctcac ccactcatcc ttacacacaa aagctactca acagtgaacc gtcaggcgat
     7441 ccggtaccgt tgccagaacc tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc
     7501 ttccccattc gcaaagggat tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac
     7561 atcagtttta cgctacgggc gggtgaaaca ctgggtttag tgggcgagtc tggttccggg
     7621 aaaagcacga cgggactggc gctgctgcga ctgattacct ctcagggcag catcgtcttt
     7681 gacggtcagc cactgcaaga tttaaatcgc cgccagctgt tacctactcg tcatcgtatt
     7741 caggtggtat ttcaggatcc aaactcctcg ctcaacccac gactcaacgt tttgcagatt
     7801 attgaggaag gcttacgggt tcaccagccg acgctttctg ccgcacaacg cgaacaacaa
     7861 gtgatagccg tgatgcatga agtgggatta gatcctgaaa cacgccaccg ttatccggcg
     7921 gagttctctg gtggtcagcg acaacgtatt gcgattgcca gggcattaat tcttaagccc
     7981 tcactgatca tacttgatga accgacatca tcactcgaca aaacggtaca ggcgcaaata
     8041 ttgacgctat tgaaatcatt gcaacaaaag catcaactgg cctatttgtt tatcagccac
     8101 gatttgcacg ttgtccgcgc gttatgtcat caggttatcg tactgcgaca aggggaagta
     8161 gtggaacaag gaccgtgcgc gcgcgtgttt gccgcaccgc agcaggagta tacgcgtcag
     8221 ctactggcgt tgagctgacg cttaaaaagg attgtagttt gaaaagggtt cggcaattgc
     8281 cacaccaaaa tttttcaatc ggcatgttgc agcaaactcg tcctggcgat ttacaaacag
     8341 gcacggctca ccttcacact ccagcaccga acatggaact tcgatgtcgc ttaacgcctg
     8401 gcttagttta tacatcaccg accatgcatt atcaccatcc gggctaagca atttaagcgc
     8461 caccaggctg ttgtcaccgc acggtccatt ttgcggaatc ggttcaacct tcgaacctgc
     8521 gaaaccagca gaccagcgaa agttctgcgg tagacgatgg acgattgaga gttgtaatga
     8581 agtcacttat tttccccgga agcacattta cttcacaatt tatttacatc aattttaaca
     8641 catcatcaac aatccgtctt tatacagatc gtaaagattg atgcctcgcc tgcatggcct
     8701 ctatggctct gatttaagta atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac
     8761 ccgtttctgc gatctaactc aaccttttta actac
//
LOCUS       DAZSBF010000168         8722 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZSBF010000168 DAZSBF010000000
VERSION     DAZSBF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.171"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(278..541)
                     /gene="rpsT"
                     /locus_tag="ACHXWD_003154"
     CDS             complement(278..541)
                     /gene="rpsT"
                     /locus_tag="ACHXWD_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR7621996.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            644..862
                     /locus_tag="ACHXWD_003155"
     CDS             644..862
                     /locus_tag="ACHXWD_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR7621997.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            870..1811
                     /gene="ribF"
                     /locus_tag="ACHXWD_003156"
     CDS             870..1811
                     /gene="ribF"
                     /locus_tag="ACHXWD_003156"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR7621998.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQKEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1854..4670
                     /gene="ileS"
                     /locus_tag="ACHXWD_003157"
     CDS             1854..4670
                     /gene="ileS"
                     /locus_tag="ACHXWD_003157"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR7621999.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4670..5164
                     /gene="lspA"
                     /locus_tag="ACHXWD_003158"
     CDS             4670..5164
                     /gene="lspA"
                     /locus_tag="ACHXWD_003158"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR7622000.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5289..5738
                     /gene="fkpB"
                     /locus_tag="ACHXWD_003159"
     CDS             5289..5738
                     /gene="fkpB"
                     /locus_tag="ACHXWD_003159"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR7622001.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5740..6690
                     /gene="ispH"
                     /locus_tag="ACHXWD_003160"
                     /gene_synonym="lytB"
     CDS             5740..6690
                     /gene="ispH"
                     /locus_tag="ACHXWD_003160"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR7622002.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6756..7670
                     /gene="rihC"
                     /locus_tag="ACHXWD_003161"
     CDS             6756..7670
                     /gene="rihC"
                     /locus_tag="ACHXWD_003161"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR7622003.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7837..8658
                     /gene="dapB"
                     /locus_tag="ACHXWD_003162"
     CDS             7837..8658
                     /gene="dapB"
                     /locus_tag="ACHXWD_003162"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR7622004.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         2000 a         2169 c         2467 g         2086 t
ORIGIN      
        1 cttaagttta atacctcaat gtgtatcaca atatccacat tctttgtagg ggagtctgga
       61 gattgagtag atattcttgt tcagaatgta tcagccgatg gttctacgat tcttaagcca
      121 cgaagagttc agaaagtgta acggcatgtc tgcaaaagcc agcatctttt gactatctgg
      181 caaccggtag tgtgttctct cacgcatcat aaaagcagca ggcataaaaa aaacccgctt
      241 gcgcgggctt tttcacaaag cttcagcaaa ttggcgatta agccagtttg ttgatctgtg
      301 cagtcaggtt agccttatga cgtgcagctt tgtttttgtg gatcagacct ttagcagcct
      361 gacggtccac gatcggttgc atttcgttaa atgctttctg tgcagcagct ttgtcgccag
      421 cttcgatagc tgcgtatact ttcttgatga aagtacgcat catagagcga cggcttgcgt
      481 tgtgcttacg agccttttca gactgaatgg cgcgcttctt agctgatttg atattagcca
      541 aggtccaact cccaaatgtg ttctatatgg acaattcaaa ggccgaggaa tatgcccttt
      601 tagccttctt ttgtcaatgg atttgtgcaa ataagcgccg ttaatgtgcc ggcactcgtt
      661 acgtagtgat ggcgcaggat tctaccagct tgcggggtgt gaatacagct tttccgcgat
      721 aaaaattgca gcaggcggtc actttcttcc cgtgatttgc gccatggcaa tgaaaagcca
      781 cttctttctg atttcggtac tcaatcgccg gttaaccttg accgctgtac aaggtatact
      841 cggacgattt tcactgtttt gagccagaca tgaagctgat acgcggcata cataatctca
      901 gccaggcccc gcaagaaggg tgtgtgctga ctattggtaa tttcgacggc gtgcatcgcg
      961 gtcatcgcgc gctgttacag ggcttgcaga aagaagggcg caagcgcaac ttaccggtga
     1021 tggtgatgct ttttgaacct caaccactgg aactgtttgc taccgataaa gccccggcaa
     1081 gactgacccg gctgcgggaa aaactgcgtt accttgcaga gtgtggcgtt gattacgtgc
     1141 tgtgcgtgcg tttcgacagg cgtttcgcgg cgttaaccgc gcaaaatttc atcagcgatc
     1201 ttctggtgaa gcatttgcgc gtaaaatttc ttgccgtagg ggatgatttc cgctttggcg
     1261 ctggtcgtga aggcgatttc ttgttattac agaaagctag catggaatac ggcttcgata
     1321 tcactagtac gcaaactttt tgcgaaggtg gcgtgcgtat tagcagcacc gctgtgcgtc
     1381 aggcgctggc ggatgacaat ctggctctgg cagaaagttt actggggcac ccgtttgcca
     1441 tctccgggcg tgtagtccac ggtgatgaat tagggcgcac tataggtttc ccgacggcga
     1501 atgtaccgct acgccgtcag gtttccccgg tgaaaggggt ttatgcggta gaagtgctgg
     1561 gcctcggtga aaagccgtta cccggcgtgg caaacatcgg aacacgccca acggttgccg
     1621 gtattcgcca gcagctggaa gtgcatttgt tagatgttgc aatggacctt tacggtcgcc
     1681 atatacaagt agtgctgcgt aaaaaaatac gcaatgagca gcgatttgcg tcgctggacg
     1741 aactgaaagc gcagattgcg cgtgatgaat taaccgcccg cgaatttttt gggctaacaa
     1801 aaccggctta agcctgttat gtaatcaaac cgaaacacgg aaccgagaat ctgatgagtg
     1861 actataaatc aaccctgaat ttgccggaaa cagggttccc gatgcgtggc gatctcgcca
     1921 agcgcgaacc gggaatgctg gcgcgttgga ctgatgatga tctgtacggc atcatccgtg
     1981 cggctaaaaa aggcaaaaaa accttcattc tgcatgatgg ccctccttat gcgaatggca
     2041 gcattcatat tggtcactcg gttaacaaga ttctgaaaga cattatcgtg aagtccaaag
     2101 ggctttccgg ttatgactcg ccgtatgtgc ctggctggga ctgccacggt ctgccgatcg
     2161 agctgaaagt agagcaagaa tacggtaagc cgggtgagaa attcaccgcc gccgagttcc
     2221 gcgccaagtg ccgcgaatac gcggcgaccc aggttgacgg tcaacgcaaa gactttatcc
     2281 gtctgggcgt gctgggcgac tggtcgcacc cgtacctgac catggacttc aaaactgaag
     2341 ccaacatcat ccgcgcgctg ggcaaaatca tcggcaacgg tcacctgcac aaaggcgcga
     2401 agccagttca ctggtgcgtt gactgccgtt ctgcgctggc ggaagcggaa gttgagtatt
     2461 acgacaaaac ttctccgtcc atcgacgttg cttttcaggc ggtcgatcag gatgcactga
     2521 aagcaaaatt tgccgtaagc aacgttaacg gcccaatctc gctggtgatc tggaccacta
     2581 cgccgtggac tctgcctgcg aaccgcgcaa tctctattgc acctgatttc gactatgcgc
     2641 tggtgcagat cgacggtcag gccgtgattc tggcgaaaga tctggttgaa agcgtaatgc
     2701 agcgtatcgg cgtgaccgat tacaccattc tcggcacggt aaaaggtgcg gagcttgagt
     2761 tgctgcgctt tacccatccg tttatgggct tcgacgttcc ggcaatcctc ggcgatcacg
     2821 ttaccctaga tgccggtacc ggtgccgttc acaccgcgcc tggccacggc ccggacgact
     2881 atgtgatcgg tcagaaatac ggcctggaaa ccgctaaccc ggttggcccg gacggcactt
     2941 atctgccggg cacttatccg acgctggatg gcgtgaacgt cttcaaagcg aacgacatcg
     3001 tcgttgcgct gctgcaggaa aaaggcgcgc tgctgcacgt tgagaaaatg cagcacagct
     3061 atccgtgctg ctggcgtcac aaaacgccga tcatcttccg cgcgacgccg cagtggttcg
     3121 tcagcatgga tcagaaaggt ctgcgtgcgc agtcactgaa agagatcaaa ggcgtgcagt
     3181 ggatcccgga ctggggccag gcgcgtatcg agtcgatggt tgctaaccgt cctgactggt
     3241 gtatctcccg tcagcgcacc tggggcgtac cgatgtcact gttcgtgcac aaagacacgg
     3301 aagagctgca tccgcgtact ctcgaactaa tggaagaagt ggcaaaacgc gttgaagttg
     3361 acggcatcca ggcgtggtgg gatcttgatg cgaaagagat cctcggcgat gaagctgatc
     3421 agtacgtgaa agtgccggac acattggatg tatggtttga ctccggatct acccactctt
     3481 ctgttgttga cgtgcgtccg gaatttgccg gtcacgcagc ggacatgtat ctggaaggtt
     3541 ctgaccagca ccgtggttgg ttcatgtctt ccctaatgat ctccaccgcg atgaaaggca
     3601 aagcgccgta tcgtcaggta ctgacccacg gctttaccgt ggatggtcag ggtcgcaaga
     3661 tgtctaaatc catcggcaat accgtttcgc cgcaggatgt gatgaacaaa ctgggcgcgg
     3721 atattctgcg tctgtgggtg gcatcaaccg actacaccgg tgaaatggcc gtttctgacg
     3781 agatcctgaa acgtgctgcc gatagctatc gtcgtatccg taacaccgcg cgcttcctgc
     3841 tggcaaacct gaacggtttt gatccagcaa aagatatggt gaaaccggaa gagatggtgg
     3901 tactggatcg ctgggccgta ggttgtgcga aagcggcaca ggaagacatc ctcaaggcgt
     3961 acgaagcata cgatttccac gaagtggtac agcgtctgat gcgcttctgc tccgttgaga
     4021 tgggttcctt ctacctcgac atcatcaaag accgtcagta caccgccaaa gcggacagtg
     4081 tggcgcgtcg tagctgccag actgcgctgt atcacatcgc agaagcgctg gtgcgctgga
     4141 tggcaccaat cctctccttc accgctgatg aagtgtgggg ctacctgccg ggcgaacgtg
     4201 aaaaatacgt cttcaccggt gagtggtacg aaggcctgtt tggcctggca gacagtgaag
     4261 cgatgaacga tgcgttctgg gacgagctgt tgaaagtgcg tggcgaagtg aacaaagtca
     4321 ttgagcaagc gcgtgccgac aagaaagtgg gtggctcgct ggaagcggca gtaaccttgt
     4381 atgcagaacc ggaactgtcg gcgaaactga ccgcgctggg cgatgaatta cgatttgtcc
     4441 tgttgacctc cggcgctacc gttgcagact ataacgacgc acctgctgat gctcagcaga
     4501 gcgaagtact caaagggctg aaagtcgcgt tgagtaaagc cgaaggtgag aagtgcccac
     4561 gctgctggca ctacacccag gatgtcggca aggtggcgga acacgcagaa atctgcggcc
     4621 gctgtgtcag caacgtcgcc ggtgacggtg aaaaacgtaa gtttgcctga tgagtcaatc
     4681 gatctgttca acagggctac gctggctgtg gctggtggta gtcgtgctga ttatcgatct
     4741 gggcagcaaa tacctgatcc tccagaactt tgctctgggg gatacggtcc cgctgttccc
     4801 gtcgcttaat ctgcattatg cgcgtaacta tggcgcggcg tttagtttcc ttgccgatag
     4861 cggcggctgg cagcgttggt tctttgccgg tattgcgatt ggtattagcg tgatcctggc
     4921 agtgatgatg tatcgctcga aggccacgca gaagctaaac aatatcgctt acgcgctgat
     4981 tattggcggc gcgctgggca acctgttcga ccgcctgtgg cacggcttcg ttgtcgatat
     5041 gatcgacttc tacgtcggcg actggcactt cgccaccttc aaccttgccg atactgccat
     5101 ctgtgtcggt gcggcactga ttgtgctgga aggttttttg ccttctagag cgaaaaaaca
     5161 ataataaacc ctgccggatg cgatgctgac gcatcttatc cggcctacag attgctgcga
     5221 aatcgtaggc cggataaggc gtttacgccg catccggcaa aaatcctcaa atataagagc
     5281 aaacctgcat gtctgaatct gtacagagca atagcgccgt cctggtgcac ttcacgctaa
     5341 aactcgacga tggcaccacc gccgagtcta cccgcaacaa cggtaaaccg gcgctgttcc
     5401 gcctgggtga tgcttctctt tctgaagggc tggagcaaca cctgttgggg ctgaaagtgg
     5461 gcgataaaac caccttctcg ttggagccag atgcggcgtt tggcgtgccg tcaccggacc
     5521 tgattcagta cttttcccgc cgtgaattta tggatgcagg cgagccagaa attggcgcaa
     5581 tcatgctttt taccgcaatg gatggcagtg agatgcctgg cgtgatccgc gaaattaacg
     5641 gcgactccat taccgttgat ttcaaccatc cgctggccgg gcagaccgtt cattttgata
     5701 ttgaagtgct ggaaatcgat ccggcattgg aggcgtaaca tgcagatcct gttggccaac
     5761 ccgcgtggtt tttgtgccgg ggtagaccgc gctatcagca ttgttgaaaa cgcgctggcc
     5821 atttacggcg caccgatata tgtccgtcac gaagtggtac ataaccgcta tgtggtcgat
     5881 agcttgcgtg agcgtggggc tatctttatt gagcagatta gcgaagtacc ggacggcgcg
     5941 atcctgattt tctccgcaca cggtgtttct caggcggtac gtaacgaagc aaaaagccgc
     6001 gatttgacgg tgtttgatgc cacctgtccg ctggtgacca aagtgcatat ggaagtcgcc
     6061 cgcgccagcc gtcgtggcga agagtcgatt ctcatcggcc acgccgggca tccggaagtg
     6121 gaaggtacaa tgggccagta cagtaacccg gaagggggaa tgtatctggt cgaatcgccg
     6181 gacgatgtgt ggaaactgac ggtcaaaaac gaagagaagc tctcctttat gacccagacc
     6241 acgctgtcgg tggatgacac gtctgatgtg atcgacgcgc tgcgtaaacg cttcccgaaa
     6301 attgtcggtc cgcgcaaaga tgacatctgc tacgccacga ctaaccgtca ggaagcggta
     6361 cgcgccctgg cagaacaggc ggaagttgtg ttggtggtcg gttcgaaaaa ctcctccaac
     6421 tccaaccgtc tggcggagct ggcccagcgt atgggcaaac gcgcgttttt gattgacgat
     6481 gcgaaagata tccaggaaga gtgggtgaaa gaggttaaat gcgtcggcgt gactgcgggc
     6541 gcatcggctc cggatattct ggtgcaaaat gtggtggcac gtttgcagca gctgggcggt
     6601 ggtgaagcca ttccgctgga aggccgtgaa gaaaacattg ttttcgaagt gccgaaagag
     6661 ctgcgtgtcg atattcgtga agtcgattaa gtcattagca gcctaagtta tgcgaaaatg
     6721 ccggtcttgt taccggcatt ttttatggag aaaacatgcg tttacctatc ttcctcgata
     6781 ctgaccccgg cattgacgat gccgtcgcca ttgccgccgc gatttttgcc cccgaactcg
     6841 acctgcaact gatgaccacc gtcgcgggta atgtctcggt tgagaaaacc acccgcaacg
     6901 ccctgcaact gctgcatttc tggaatgcgg agattccgct cgcccaggga gccgctgtgc
     6961 cgctggtacg cgcaccgcgt gatgcagcat ctgtgcacgg cgaatcggga atggctggct
     7021 acgactttgt tgagcacaac cgaaagccaa tcgggatacc agcgtttctg gcgattcgtg
     7081 atgccctgat gcgtgcacca gagcctgtta ccctggtggc cattggcccg ttaaccaata
     7141 ttgcgctgtt actttcacaa tgcccggaat gtaaaccgta tattcgtcgt ctggtgatca
     7201 tgggcggttc ttctggtcgc ggcaactgta caccaaatgc cgagtttaat atcgctgccg
     7261 atccagaagc ggctgcctgt gtcttccgta gcggaatcga aatcgtcatg tgcggtctgg
     7321 atgtcaccaa tcaggcaata ttaacccctg actatctcgc tactttgccg gagttgaatc
     7381 gcaccggaaa aatgcttcat gcactgttta gccactaccg tagcggcagt atgcaaagcg
     7441 gtctgcgaat gcacgatctc tgcgccatcg cctggctggt gcgcccggac ttattcaccc
     7501 tcaagccgtg ttttgtcgcg gtagaaactc aaggcgaatt tacctcaggc acgacggtgg
     7561 ttgatatcga cggttgcctg ggcaaaccag ccaatgtaaa ggtggcattg gatctggatg
     7621 tgaaaggctt ccagcagtgg gtggctgagg tgctggctct ggcgtcgtaa cctgtcacat
     7681 gttatcggca tgcagtcatt catcgactca tgcctttcac tgatatccct ccctgtttat
     7741 cattaatttc taattatcag cgtttttggc tggcggcgta gcgatgcgct ggttactctg
     7801 aaaacggtct atgcaaatta acaaaagaga atagctatgc atgatgcaaa catccgcgtt
     7861 gccatcgcgg gagccggggg gcgtatgggc cgccagttga ttcaggcggc gctggcatta
     7921 gaaggcgtgc agctgggcgc ggcgctggag cgcgaaggat cttctttact gggcagcgac
     7981 gccggtgagc tggctggtgc agggaaaaca ggcgttaccg tgcaaagcag cctcgatgcg
     8041 gtaaaagatg attttgatgt gtttatcgat tttacccgtc cggaaggtac gctgaaccat
     8101 ctcgcttttt gtcgccagca tggcaaaggg atggtgatcg gcactacggg gtttgacgaa
     8161 gccggtaaac aggcgattcg tgacgccgcc gccgatattg cgattgtctt tgccgccaat
     8221 tttagcgttg gcgttaacgt catgcttaag ctgctggaga aagcagccaa agtgatgggt
     8281 gactacaccg atatcgaaat tattgaagca catcatagac ataaagttga tgcgccgtca
     8341 ggcaccgcac tggcaatggg agaggcgatc gcccacgccc ttgataaaga tctgaaagat
     8401 tgcgcggtct acagtcgtga aggccacacc ggtgaacgtg tgcctggcac cattggtttt
     8461 gccaccgtgc gtgcaggtga catcgttggt gaacataccg cgatgtttgc cgatattggc
     8521 gagcgtctgg agatcaccca taaggcgtcc agccgtatga catttgctaa cggcgcggta
     8581 agatcggctt tgtggttgag tggtaaggaa aatggtcttt ttgatatgcg agatgtactt
     8641 aatctcaata atttgtaacc acaaaatatt tgttatggtg caaaaataac acatttaatt
     8701 tattgattat aaagggcttt aa
//
LOCUS       DAZSBF010000169         8634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZSBF010000169 DAZSBF010000000
VERSION     DAZSBF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.172"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHXWD_003163"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHXWD_003163"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR7622005.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHXWD_003164"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHXWD_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR7622006.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHXWD_003165"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="ACHXWD_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR7622007.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHXWD_003166"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="ACHXWD_003166"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR7622008.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHXWD_003167"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="ACHXWD_003167"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR7622009.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHXWD_003168"
     CDS             complement(7826..8137)
                     /gene="ymdA"
                     /locus_tag="ACHXWD_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR7622010.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHXWD_003169"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="ACHXWD_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR7622011.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2179 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaa
//
LOCUS       DAZSBF010000170         8634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZSBF010000170 DAZSBF010000000
VERSION     DAZSBF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.173"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(213..1877)
                     /gene="glnS"
                     /locus_tag="ACHXWD_003170"
     CDS             complement(213..1877)
                     /gene="glnS"
                     /locus_tag="ACHXWD_003170"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR7622012.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2137..4083)
                     /gene="nagE"
                     /locus_tag="ACHXWD_003171"
     CDS             complement(2137..4083)
                     /gene="nagE"
                     /locus_tag="ACHXWD_003171"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR7622013.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4416..5216
                     /gene="nagB"
                     /locus_tag="ACHXWD_003172"
     CDS             4416..5216
                     /gene="nagB"
                     /locus_tag="ACHXWD_003172"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR7622014.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5276..6424
                     /gene="nagA"
                     /locus_tag="ACHXWD_003173"
     CDS             5276..6424
                     /gene="nagA"
                     /locus_tag="ACHXWD_003173"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR7622015.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6433..7653
                     /gene="nagC"
                     /locus_tag="ACHXWD_003174"
                     /gene_synonym="nagR"
     CDS             6433..7653
                     /gene="nagC"
                     /locus_tag="ACHXWD_003174"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR7622016.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7701..8453
                     /gene="nagD"
                     /locus_tag="ACHXWD_003175"
     CDS             7701..8453
                     /gene="nagD"
                     /locus_tag="ACHXWD_003175"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR7622017.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8504..8634)
                     /locus_tag="ACHXWD_003176"
                     /pseudo
     CDS             complement(8504..8634)
                     /locus_tag="ACHXWD_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2108 a         2274 c         2201 g         2051 t
ORIGIN      
        1 tgaacgcggc gttttggatt gtttgcatat ttcttgccgg atgcggcgtg aacgccttaa
       61 ccggcctaca aaagcacgca aactcaatat attgcagaga tcatgtaggc ctgataagcg
      121 tagtgcatca ggcaatttag cgtttgaaac tgtttgtctg ttccttgccg gatgcgacgt
      181 aaacgcccca tccggcatag cgaaacttaa aattactcgc ctactttcgc ccaggtatca
      241 cgcagcccaa cggtgcggtt aaataccggt ttttccgccg tagaatggcg gctatcgagg
      301 cagaagtaac cttcacgctc aaactggaat gctttacccg caaccgcatc tttcagcgac
      361 ggttcagcaa agccctgttt gatcaccagc gattccgggt taatcaccga caggaaatca
      421 tccgcagcac cagggttcgg cacgctgaac agacggtcgt acagacggat ttcaaccggc
      481 agcgcatgtg ccgcgctcac ccagtgaata acacctttga ctttacgacc atctgccgga
      541 tctttgctta aggtgtcggc gtcataagta cagaagatgg tggtgatatt gccttcggca
      601 tctttctcca cgcgttctgc cttaatgacg taagcattac gcagacgcac ttctttaccc
      661 agcaccagac gtttgtactg cttgttagct tcttcgcgga aatcggcgcg atcgatccaa
      721 atctcaccgc taaacggcac ctggcggcta cccatttccg gtttgttcgg atggttcggc
      781 atggtaacca tttcgccttc gccctgatag ttttcgataa ccagtttcac cggatcgata
      841 accgccattg cgcgcggggc gttttcgttg agatcttcac ggatgcagga ttccagcgac
      901 gccatctcaa tggtgttgtc ctgcttggtc acgccgatgc gtttgcagaa ctcacgaata
      961 gaagcagcag tgtaaccacg acgacgcaga ccggaaatgg tcggcatacg cgggtcatcc
     1021 cagccttcaa cgtgcttgtc ggtcaccagc aggttcagct tacgcttgga catcacagtg
     1081 tattccagat tcaggcgcga gaattcgtac tggcgcgggt gaacaggaat ggtgatgttg
     1141 tccagcaccc agtcgtacag gcgacggttg tcctggaact caagcgtaca cagagagtgc
     1201 gtaatacctt ccagggcatc gctgatgcag tgggtgaagt cgtacatcgg gtagatgcac
     1261 cacttgttgc cagtctggtg gtgttcagca aatttaatgc ggtacagcac cggatcgcgc
     1321 atcacgataa acggtgaagc catgtcgatt ttcgcacgca ggcaggcttt accttcttca
     1381 aaaccaccgg cacgcatttt ttcgaacagc gccaggttct cttcaacgct gcggtcgcgg
     1441 tacgggctgt ttttacccgg ttgcgtcagg gtgccgcggt attcgcggat ctgttccggc
     1501 gtcagttcat caacgtacgc caggccttta ttgatcagtt cgatcgcata ggcgtggagc
     1561 tgatcaaaat aatcggagga gtaacggacg ttaccagacc agtgaaaacc taaccactct
     1621 acgtcgtttt tgatcgactc aacatactcg atatcttctt ttaccgggtt agtgtcgtcg
     1681 aaacgcaggt tgcactggcc tttatagtcc tgggcgatcc cgaagttcag gcagatagat
     1741 ttcgcatggc caatatgcag atagccattc ggctccggcg ggaaacgggt gtgtactgtg
     1801 gtgtgcttac cactggccag atcttcatcg atgatctgac ggataaagtt agtcgggcgg
     1861 gcttctgcct cactcatcgt ggattcctca aagcgtaaac aacgtataac ggcgtatgat
     1921 cttataagcg ggacaggctg acaactgtta gttagcgaaa atcgaaaatc tcactgagat
     1981 tatggggatg attgatgaaa aaaaaaagcg gcagagaaga tctccaccgc ttttgccagt
     2041 cccgcgtcat tgttggatgc gcgtcttatc caaccaggac tgagcttttg taggcctgat
     2101 aagacgcgtc aagcgtcgca tcaggcataa agcagattac tttttgattt catacagcgg
     2161 tgtttgaccc gccacaacat tgccctgagc tttaatgatc aagccactga aatcgtcgat
     2221 attgctgcaa accaccgggc taatcatcga acgggcgtta gcgttcaggt aatccagatc
     2281 catttccaga atcggttgcc ctgcgctcac ctgcgcgccc tcttccacca gacgtttaaa
     2341 gcctttacct tccagcgcta cggtgtcgat acccatgtgg acgacaatct ccgcgccttt
     2401 ttcggtttct aggcagaacg cgtggttggt gttgaagatt ttcacgattg tccctgcggc
     2461 tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca ccgctttgct
     2521 ggcgaatgct tcgtcagaaa cctgatccag tgccacgaca tcaccggtaa tcggcgatac
     2581 cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag gagcggcagt
     2641 tgccggagtt gcttcagctg acgcagctgc taccgggcca cgggcaacga ctttcttcat
     2701 cgcatcgcca atggattctg ctttcgcgcc aacaatcacc tgaatggtct gtttgttcag
     2761 ttttactaca ccagaagcac ccagacgttt acacatcgca tcgttgacgc gagctgagtc
     2821 agccacggta aggcgcagac gggtaataca ggcgtcaatc gctttcaggt tgtcagtacc
     2881 gccaaccgca gcaatatagt tggttgccag ttgagtcaga ccttcttcag tattgctgtt
     2941 agcctcttca gttacgatct cgtcttcttt atcttcacga cccggcgttt tcaggttgaa
     3001 catgcggata accaaactga acaccacgaa gtagatagcg aagaagacaa cacccatcac
     3061 cagcagcatc cagacgttct ggctggcggc cggcaggtta tacatcaacg cgtagtcgat
     3121 agcccccgca gagaaagaga agccagcatg gatacccagc agcgttgcca caaacaggct
     3181 gataccggtc agcagtgcgt gcaggaggta cagcagcgga gcaaggaaca tgaacaggaa
     3241 ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa gcagcatccc
     3301 gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg ccgcacccgg
     3361 cagaccgaac atcatgatcg ggaagaagcc ggacatgaac atccccgcgg tgccgtcacc
     3421 agcgtagaaa cggttgatgt cgccgtggaa aaccgtaccc gccgcgttgg tgaattcacc
     3481 aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga tcagcagacg
     3541 gttgatgaaa ccaaagatac cggaacccag cgcgcctgca gaaacgatcc actcgccgcc
     3601 tgcatggata gcgtgctgta ctggcggcca gacgtaacca aaaatggccg ccagcaccag
     3661 gcagaaaaag ccggtggcaa tcggcacaaa gcgtttgccg ccgaagaagc tcaggaagtc
     3721 cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac cggtaatgat
     3781 acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt tggttaacac
     3841 aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg accagctgga
     3901 tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa atcgcaccgc ccgcctgggc
     3961 aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagtg ccgccaccgg
     4021 cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac ctaaaatatt
     4081 catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt tttattttgc
     4141 tgcaaattgt actgccgatg ttctgtaatc agattgttag atcatctgct acagagtgtg
     4201 tgaaaattta attcgtatcg caaattaaac gcgtgccttt tgtgagtttt gtcaccaaat
     4261 atcgttatca tcactccctt ttactggcta aagcagaaaa cttattttat cattcaaaaa
     4321 atcaggtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga tgcctaatcc
     4381 gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc ctaactaccg
     4441 ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat gcgttcaaac
     4501 cgactgccga tcgtccgttt gttctgggcc tgccgactgg cggcacgccg atgaccacct
     4561 ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac gttgtcacct
     4621 tcaacatgga cgaatatgtc ggtctgccga aagagcatcc ggaaagctac tacagcttta
     4681 tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac cttctcaacg
     4741 gcaacgcccc ggatatcgac gccgaatgcc gccagtatga agaaaaaatc cgttcttacg
     4801 gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca tttaacgaac
     4861 cggcgtcttc tctggcttct cgtactcgta tcaaaaccct gactcatgac actcgcgtcg
     4921 cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc ctgactgtcg
     4981 gtgttggtac attgctggat gccgaagaag tgatgattct ggtgctgggt agccagaaag
     5041 cactggcact gcaggctgca gttgaaggtt gcgtgaacca catgtggacc atcagctgtc
     5101 tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc ttccaccatg gagctgaaag
     5161 ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt ctgtaattgt
     5221 tatccctgcc ctggctcctt gctcagggca atatttttta aaatcggggg gcagaatgta
     5281 tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc acgcggttgt
     5341 tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc cagagatcga
     5401 acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc agttaaacgg
     5461 ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa cgctggaaat
     5521 catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc ttatcaccac
     5581 cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc gagtacctgg caagacatcc
     5641 gaatcaggcg ttaggtctgc atctggaagg tccgtggctg aatctggtaa aaaaaggcac
     5701 ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc tgtgtgaaaa
     5761 cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg aagtcatcag
     5821 caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga cgttgaaaga
     5881 agcgaaagcc ggtttccgcg cggggattac ctttgccacc catctgtaca acgcgatgcc
     5941 gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgacgaag ctgacattta
     6001 ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc aacattcgta acgctaaacg
     6061 ccttaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag gtgccaacat
     6121 tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt gtgtggatga
     6181 gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta atctggtcga
     6241 acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc cggcgcgtgc
     6301 gattggcgtt gagaaacgtc tcggcacact cgccgcaggt aaagtagcca acctgacctc
     6361 attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg tcgtaactca
     6421 ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga tctcgtaaaa
     6481 cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat ctcgcggatt
     6541 cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac gcgtcagctt
     6601 atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg ccgccgcgct
     6661 atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct tggtcgtcac
     6721 gacgccacca tcaccctgtt tgatctcagc agcaaagtgc tggcagaaga acattacccg
     6781 ctgccggaac gtacccagca aacgctggaa catgccctgt tgaatgccat tgctcagttt
     6841 attgatagct accagcgcaa actgcgcgag ctgatcgcga tttcggtgat cctgccaggg
     6901 cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt agaaaactgg
     6961 gggctggtag aagctctgga agaacgtttt aaagtgacct gtttcgttgg tcacgatatc
     7021 cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga agactccatt
     7081 ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg catttttatt
     7141 ggccgcaacg gtaacgtcgg tgaaattggc catattcagg tcgaaccgct gggtgaacgc
     7201 tgccactgcg gcaactttgg ctgcctggaa actatcgccg ccaacgctgc cattgaacaa
     7261 cgggtgttga atctgttaaa gcagggctac cagagccgcg tgccgctgga cgactgcacc
     7321 atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagtc tggcgtcgga agtaattgag
     7381 tatgtcggtc gtcatctggg taaaaccatc gccattgcta tcaacttatt taatccgcaa
     7441 aaaattgtta ttgccggtga aatcaccgaa gccgataaag tgctgctccc tgctattgaa
     7501 agctgcatta atacccaggc gctgaaggcg tttcgtacta atctgccggt ggtacgttct
     7561 gagctggatc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc catgctcaac
     7621 ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc gcttattgtt
     7681 gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc gacggcgtgc
     7741 tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg attatggata
     7801 aaggcctgcc gctggtgttg ctgaccaact atccttcgca gaccgggcaa gatctggcga
     7861 accgctttgc cactgccggt gtcgatgtgc cggatagcgt gttttatacc tctgcgatgg
     7921 cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc atatgtggtg ggcgaaggcg
     7981 cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac cctgatttcg
     8041 tgattgttgg cgaaacgcgt tcctacaact gggacatgat gcataaagcg gcctatttcg
     8101 tcgctaacgg tgcacgcttt atcgccacca acccggacac ccacgggcgc ggtttttatc
     8161 ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa ccgttctatg
     8221 ttggtaagcc cagcccgtgg atcatccgcg cagcattaaa caaaatgcag gcgcattcgg
     8281 aagaaacggt gattgtcggc gataacttgc gcaccgacat tctggcgggc ttccaggcag
     8341 gtctggaaac gattctggtg ctttctggtg tttcatcgct cgacgatatc gacagtatgc
     8401 cgttccgccc aagctggatt tacccgtcag tcgctgaaat cgacgttatc tgaataccac
     8461 ccacgtctgc ggacgtggtt ggctctgatg acaaacgcca aactgcctga tgcgctacgc
     8521 ttatcaggcc tacgcagctc ctgcaatata ttgaattttc atgcttttgt aggccggata
     8581 aggcgttcac gccgcatccg gcatgaacaa atcgcacttt atcagcaatt tggc
//
LOCUS       DAZSBF010000171         8533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZSBF010000171 DAZSBF010000000
VERSION     DAZSBF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.174"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHXWD_003177"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="ACHXWD_003177"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR7622018.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHXWD_003178"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="ACHXWD_003178"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR7622019.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="ACHXWD_003179"
     CDS             complement(1322..2986)
                     /locus_tag="ACHXWD_003179"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR7622020.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /locus_tag="ACHXWD_003180"
                     /pseudo
     CDS             3028..3861
                     /locus_tag="ACHXWD_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="ACHXWD_003181"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="ACHXWD_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR7622021.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHXWD_003182"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="ACHXWD_003182"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR7622022.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPHWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="ACHXWD_003183"
     CDS             8269..>8533
                     /locus_tag="ACHXWD_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7622023.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1941 a         2390 c         2334 g         1868 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcactg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAZSBF010000172         8488 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZSBF010000172 DAZSBF010000000
VERSION     DAZSBF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.175"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            157..1293
                     /locus_tag="ACHXWD_003184"
     CDS             157..1293
                     /locus_tag="ACHXWD_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR7622024.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(1335..2105)
                     /gene="yafV"
                     /locus_tag="ACHXWD_003185"
     CDS             complement(1335..2105)
                     /gene="yafV"
                     /locus_tag="ACHXWD_003185"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR7622025.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVAWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            2286..2732
                     /gene="ivy"
                     /locus_tag="ACHXWD_003186"
     CDS             2286..2732
                     /gene="ivy"
                     /locus_tag="ACHXWD_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR7622026.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2775..5219)
                     /gene="fadE"
                     /locus_tag="ACHXWD_003187"
     CDS             complement(2775..5219)
                     /gene="fadE"
                     /locus_tag="ACHXWD_003187"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR7622027.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5459..6037
                     /gene="lpcA"
                     /locus_tag="ACHXWD_003188"
     CDS             5459..6037
                     /gene="lpcA"
                     /locus_tag="ACHXWD_003188"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR7622028.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            6243..7010
                     /locus_tag="ACHXWD_003189"
     CDS             6243..7010
                     /locus_tag="ACHXWD_003189"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR7622029.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6981..7721)
                     /gene="dpaA"
                     /locus_tag="ACHXWD_003190"
     CDS             complement(6981..7721)
                     /gene="dpaA"
                     /locus_tag="ACHXWD_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR7622030.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7877..8155)
                     /gene="yafQ"
                     /locus_tag="ACHXWD_003191"
     CDS             complement(7877..8155)
                     /gene="yafQ"
                     /locus_tag="ACHXWD_003191"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR7622031.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(8158..8418)
                     /gene="dinJ"
                     /locus_tag="ACHXWD_003192"
     CDS             complement(8158..8418)
                     /gene="dinJ"
                     /locus_tag="ACHXWD_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR7622032.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
BASE COUNT         2172 a         2163 c         2105 g         2048 t
ORIGIN      
        1 aagactttgg agcagggaaa gatcatcgga agtacacttt ttgtactaaa taattcgcgc
       61 taaatgttca ataattgaga tattccttat tacctaaaac tatttttcat agcttatata
      121 tgatcaaata ctccttgcat aaataaggag aacaaaatgg aacttaaaaa actgatggaa
      181 catatttcta ttatccccga ttacagacaa gcctggaaag tagaacataa attgtcagat
      241 attctactgt tgactatttg tgccgtcatt tctggtgcag agggttggga agatatagag
      301 gattttggtg aaacacatct cgattttttg aagcaatatg gtgattttga aaatggtatt
      361 cctgttcacg atactattgc cagagttgta tcctgtatca gtcctgcaaa atttcacgag
      421 tgctttatta actggatgcg tgactgccat tcttcagatg ataaagacgt cattgcaatt
      481 gatggaaaaa cgctccggca ctcttatgac aagagtcgcc gcaagggagc gattcatgtc
      541 attagtgcgt tctcaacaat gcacagtctg gttctcgggc agattaagac ggatgagaaa
      601 tccaatgaga ttacagccat tcctgaactt cttaacatgc tggatattaa aggaaaaatc
      661 atcacaactg atgcgatggg atgccagaaa gatattgcag agaagataca aaaacagggc
      721 ggtgattatt tattcgcggt aaaaggaaac caggggcggc ttaataaagc ctttgaggaa
      781 aaatttccgc tgaaagaatt aaataatcca gagcatgaca gttacgcaat cagtgaaaag
      841 agtcacggca gagaagaaat ccgtcttcat attgtttgcg atgtccctga tgaacttatt
      901 gatttcacgt ttgaatggaa agggctgaag aaattatgcg tggcagtctc ctttcggtcc
      961 ataatagcag aacaaaagaa agagccagaa atgacggtca gatattatat cagttctgct
     1021 gatttaaccg cagagaagtt cgccacagca atccgaaacc actggcacgt ggagaataag
     1081 ctgcactggc gtctggacgt ggtaatgaat gaagacgact gcaaaataag aagaggaaat
     1141 gcagcagaat tattttcagg gatacggcac atcgctatta atattttgac gaatgataag
     1201 gtattcaagg cagggttaag acgtaagatg cgaaaagcag ctatggacag aaactatctg
     1261 gcgtcagtcc ttgcggagag cgggctttcg tagtcttacc ccgactctcc cccagcctta
     1321 aacacaaccc ccactcaccg caacctaaac tcatccgcat cctgccatgc cggaaacttt
     1381 tctctatatt cccgcaatgc catcatcgac agctccgcat caatgcgcgt tgcctgatgc
     1441 gcgtcggcag tagcgataat ctcgccttgt ggattaatca cccggctgtc accgcgataa
     1501 tggcagccgt tgccatcgct gccgacgcga ttgcatcccg ccacatacgc ctgattctca
     1561 atcgcgcggg ccgtcagcaa tgcctgccag tgcagagagc gcggagcagg ccagttggca
     1621 acgtagatgg cgaggtcata atcgttgaga ttgcgcgacc acaccggaaa acgtaagtcg
     1681 tagcacacca acggcaaaat acgccagccg cgccatgcca caatcactcg cgcattgccc
     1741 gctttataat gtagatgctc atctgccatg cggaacagat gacgcttatc ataaaaatgt
     1801 accgtgccgc ccggctcaac cagcaaaaag cggttaaccg aaccagactc cgtttgtaat
     1861 gcaacactgc ctgcaatcag cgcattgcac tgctgcgcct tagctgtcat ccagtccact
     1921 acgtcatctt gtgctagcga cgaagctgcc gcttccatgg caaagccgct ggtaaacatc
     1981 ttcggtagaa cgatcacatc gcgcccggta ataccttcca gttgacgatc aaaatgacgc
     2041 aggttggcag ggccatccat ccacaccagt ggttgctgca aaagcgtaat cttcaaacca
     2101 ggcacggtgt acaactcctt tatgcgaagg gttttataac tttaacacct tatcaggcag
     2161 ttgccttagc gcagaataaa ttgataacaa atgctgatat tggaaatatc tgatttgcaa
     2221 attatcgtgt tatcgccagg ctttagaagg ttaataacat gggcaggata agctcgggag
     2281 gaatgatgtt taagacaata acgacagtcg ccgctctggt catcgccacc agtgcaatgg
     2341 cgcaggatga tttaaccatt agcagccttg caaagggcga aaccaccaaa gctgcattta
     2401 atcagatggt acaagggcat aagctgcctg cctgggtgat gaaaggcggt acttatactc
     2461 ccgcacaaac cgtaacgttg ggagatgata cgtatcaggt gatgagcgcg tgcaaaccgc
     2521 atgactgtgg ctcgcaacgt atcgctgtga tgtggtccga gaaatctaat cagatgacgg
     2581 ggctgttctc gactattgat gagaaaacgt cgcaagagaa actcacctgg ctgaatgtga
     2641 acgatgcgct ttcgattgat ggtaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg
     2701 aaaaccatcc ggatggcttt aattttaaat aattagcgga taaagaaacg gagcctttcg
     2761 gctccgttat tcaattacgc ggcttcaact ttccgcactt tctccggcaa ctttaccggc
     2821 ttcgtcgcca gctcttccgg atcaaagtca tcaacgttaa tactgcgcag acggctttct
     2881 tcagctttca ccagaatagc ggcttcatct ttatcaatca gccccttcgc cagcgcgttg
     2941 tgcgccagtt catccagacg ggtaaacggc aggtttttac ccagctcttt acagatccgc
     3001 tgatgaattg ggtcggcggc aatcacatcc accagcgcct cttccagcaa gccaaccgga
     3061 ttatgctcgc tcggcgtcag gtactgaccg cgaccaatgc gggaacgggt ggcgttcggc
     3121 acttgtaaaa tcttcgccac tttatgatcc agcttgtcag aaggtgccag ataatgacgt
     3181 ccggtcggga agatcaccac attcagcagc ccggcaacca cgcggttcgg gaagttttgc
     3241 agtaaatcat ccatcgcctg ttcagcctga tacagcgcat cttgtacgcc ccagtgcacc
     3301 agcggcaggt cggcttcatt acggccttcg tcgtcataac gcttcagcac ggcagaggcg
     3361 aggtagagct ggcttaaaat atcccccaga cgggccgaga tgcgctcgcg acgtttcagg
     3421 ctgccgtcca gcactgccat cgagacatca gaaagcaggg cgaggttggc gctcaggcgg
     3481 ttcaggtgct gatagtagcg tttagtggca tcgccggttg gcgtgctgct ggttaaaccg
     3541 cgcgtcaggc ccagccagaa gctgcgaact ttgttgctac cgacgtgacc gatatgtttg
     3601 aacaacagtt tatcgaacgc gttgacgtca ttgttcttcg ccgcttccat ctcttccagc
     3661 acgtacggat ggcaacgaat cgctccttgt ccgaagatca tcatgctgcg ggtcagaatg
     3721 ttagcccctt caacggtgat ggcaatcggt gcgccctggt aagcacgcgc caggaagttg
     3781 ctttgcccga gcataatgcc tttgccacca gtgatatcca tcgcatcaat aatcgactgc
     3841 tgcccgcggt gggtacagtg atacttaacg atagccgaca gcacggcagg tttttcgccg
     3901 agcataatgc cgtaggtaat cagcgatgcc gcagcatcca tcacgtaggc attaccggca
     3961 atacgcgcca gcggctcttc aatcccttcc atcttaccga tagagatttt gaactgacgg
     4021 cgaatgtgag catacgcgcc cgttgccagc gctaccgatt tcacgccgcc ggttgagttg
     4081 gaaggcaggg tgatgccgcg gcctaccgag aggcactcca ccagcatccg ccagccttgc
     4141 ccggccattt tcggcccacc gatgatgtaa tcgatcggta cgaagacatc tttaccgcgc
     4201 gtcggtccgt tctggaacgg tacgttcagc gggaagtggc gacgaccaat ttccacgccc
     4261 ggcgtggtgg tcgggatcag cgcacaggta atacctaaat cttctgcgcc acccagcagc
     4321 ttttctgggt cggagagttt aaacgccagc ccaagcacag tcgcaatcgg tgccagcgta
     4381 atatagcgtt tgttccaggt cagacgcatc cccagcacct gctggccctg ccattcgccc
     4441 atgcagacaa tcccggtgtc cggaatcgcg cccgcatcgg aacccgcttc cgggcttgtc
     4501 agtgcaaagc aagggatctc ctgaccacgc gccagacgcg gcagatagtg atctttctgc
     4561 tcgtcagtgc cgtaatgttg cagcagctcg cccgggccta atgagttcgg cacgccgacg
     4621 gtaatcgcca ggatcccgct cacgccggag agtttttgca gcacgcgaga ctgggcataa
     4681 gctgagaact ccagtccgcc gtactctttt ttgataatca tcgcgaagaa gcgatgctct
     4741 ttaaggtacg cccacaactc cggcggcaga tccgccagct catgagtaat ctggaaatca
     4801 ttcgccatcc ggcaggcttc ttctaccggg ccgtcgagaa acgcttgctc ttcggcggtc
     4861 aggcgcggct gcggatagtt atgcagcttt ttccagtccg gcttgccctg gaacaagtcg
     4921 ccctcccacc aggtggtgcc cgcatcaatc gcttctttct cagtgcgcga catcggcggc
     4981 atcaccttac ggaaaccgcg aaataccggc gcggaaatca tcgacttacg cataggcgca
     5041 aagttaaatg gcacgaggat aatggccaga ggcaccagta cccacgctga ccacagacca
     5101 gcaacgccga gggcggctgt ccaggcgagc aaaatcagac tgctgataaa taagctcacg
     5161 cggtgataga acaacgcgcc gagcaggaca accgtagcga gaatactcaa aatcatcata
     5221 acgaaaagcc ccttacttgt aggaggtctg accacttgtg atgatatggt tgtagtggat
     5281 gtaaaaacat ttagcaatgt gtttacaata taattacaac aaagctcaca ttgttgctgt
     5341 ttttatccgc acttcaggtc aaaaagtcct ggtcatagca cctgcccgta cttctcgctt
     5401 ttggcggtat ccggtacact gcattttgtc tattacattt atgctgaagg atatcctcat
     5461 gtaccaggat cttattcgta acgaactgaa cgaagcggcg gaaacgctgg ctaacttttt
     5521 aaaagatgac gccaatattc acgccattca gcgcgcggcg gtcctgttag cagacagctt
     5581 taaagccggt ggcaaagtgc tttcctgcgg caacggcggt tcccattgcg acgctatgca
     5641 ctttgccgaa gagttgaccg gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc
     5701 tatttctgac gttagtcata tttcctgcgt cggtaatgat ttcggtttca atgatatttt
     5761 ctcccgctac gttgaagcgg taggtcgcga aggcgatgta ctgctgggga tctccacctc
     5821 cggtaactct gcaaacgtga tcaaagcgat cgcagcggcg cgtgagaagg ggatgaaagt
     5881 gatcaccctg accggtaaag acggcggtaa aatggctggc acggcggata tcgaaattcg
     5941 cgttccgcac tttggttatg ccgaccgcat tcaggagatt cacattaaag tgatccatat
     6001 cctgatccag ttaattgaaa aagagatggt taagtaagtc tggcgtaggc cggataaggc
     6061 gtttacgccg catccggcac ttgtgcgctg atgcctgatg cgacgctgat gcgtcttatc
     6121 atgcctacaa atctgtacgc gaaccgtagg ccgaataaga cgtttacgcc gcatccggcc
     6181 tgaaaattct taaatcaatc ttcgccgggg gccatgcgct cccgctgttg tggaggttac
     6241 ccatgtgcga attgctcggg atgagcgcca acgtccctac cgatatctgc tttagtttca
     6301 ccggtctggt gcagcgtggt ggtggaaccg ggccacataa agatggctgg ggcattacct
     6361 tttacgaagg taaaggctgt cgcacattta aagatccaca acccagcttt aattccccca
     6421 tcgccaaact tgtccaggac tacccgataa aatcctgttc ggtggtggct catattcgcc
     6481 aggccaatcg gggcgaggtg gcgctggaaa atactcaccc gtttacccgt gagttatggg
     6541 ggcgtaactg gacttatgcc cataacggac aactgacggg ctacaaatca ctggaaaccg
     6601 gcaacttccg cccggtcggt gaaaccgata gcgaaaaagc cttctgctgg ctcctgcata
     6661 aattaacgca gcgttacccg cgcacaccgg gcaacatggc ggcagtgttt aaatatatcg
     6721 cctcactggc ggatgaactg cggcagaagg gcgttttcaa catgctgctt tctgacgggc
     6781 gctatgtgat ggcgtattgc tcgactaatt tacactggat cacccgccgc gcgccgtttg
     6841 gcgtggcaac gctgctggat caggatgtgg aaatcgactt cagctcgcag accacaccga
     6901 atgatgtggt cacggtgatt gcaacacagc cgctgacggg caatgaaacc tggcaaaaga
     6961 ttatgccagg cgaatggcgc ttattttgcc tcggggagcg tgtagtttga tgccagttgt
     7021 ggctgcacaa cttcgtggct taacggcttg ctgaccacgt aacgaccatt gaccacagaa
     7081 acggtcggtg gcttacgggt ttgctcaaag tagtcgtaac ccggcttcag ttgctcccag
     7141 aagtccttaa agttggaata tttatggcgc ttcatattgg cgtcggtcat gcggaacggg
     7201 taaatactca cttgcacgct cggctgacca aacaccagcg caccagtaac gaactggaag
     7261 atctcatcaa taccctgatt ggtcattgcg tagcagccga tggaaacaca atcgccgtgg
     7321 atcatcaggt atttcccttc ataaccatgc gcacggtcat aggcattggg gaaaccaata
     7381 ttaatcgctt tgtagtaacg gctgtctggt tttaactgat tacgctggac gctataaaac
     7441 ccttccgggc ttttgaaatc gccctgacgc tgttttggcc ctaagccgcc ggaatattta
     7501 cagattttat aactgtcgag cagctgatat tgctcgccca ttttgacgta gagatcgagc
     7561 gtacgttctt ccttgaagat ctggatgtag acaggggatc ccatcaactg ctgcttatac
     7621 tctttgctca ctggcgtggt cgagctactg ctgcccagca aaccggcaaa cgaaacgcac
     7681 gggatcaaca acatcgcaag aattaatgcg attttacgca tactgcttat tccttgataa
     7741 aacggttaca cacgccagga cggcaaaata gatcccaaat cggaatagtc tggatttgga
     7801 aggctcacat tatcaccaaa agagttttac gcaagcctgt cggcgcaggg tttacaaatt
     7861 tcatcggcag cgtgctttac ccaaagagcg ccgcgtgagt tccagttctc tcaaatcgta
     7921 aaagtttatc ggtaagtttg taaatcagga tccagtccgg ttcgacatga gcatcgcgat
     7981 aacctttcca tgaaccttgc agcgggtggt ctttataaac agctggaagc ggtaaagcat
     8041 tattgataag aagcgtcata agatatttca atttattcat atccttatga cgcttttgtg
     8101 caagttttac atcctttgaa aattgtcccg agtattcaat atccctttga atcatattta
     8161 aattcctaat ttatcaaata aatcatcagc gtctttggcc ttatgaacat caacgccagc
     8221 ttcgctgttt ttgattgatt gaatggttaa ttgattaggc tcgcgtaaat caaacggcaa
     8281 tgccttttca cgcgcgacct ttgtgagggt tatgcgaacc aggtcagaga tggtcagccc
     8341 catcccggcc agtacgtccg ccgcctgatt cttcagatct tcatcgattc gggcgcgaac
     8401 aaacgcgtta gcagccattg ggggatctcc tgtgctgtat atttattcag cttgaattgt
     8461 agctcaaatg agcaacagcg acaatcca
//
LOCUS       DAZSBF010000173         8403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZSBF010000173 DAZSBF010000000
VERSION     DAZSBF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.176"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(82..2073)
                     /gene="tkt"
                     /locus_tag="ACHXWD_003193"
     CDS             complement(82..2073)
                     /gene="tkt"
                     /locus_tag="ACHXWD_003193"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR7622033.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2351..3109
                     /gene="loiP"
                     /locus_tag="ACHXWD_003194"
                     /gene_synonym="yggG"
     CDS             2351..3109
                     /gene="loiP"
                     /locus_tag="ACHXWD_003194"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR7622034.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3252..4172)
                     /gene="speB"
                     /locus_tag="ACHXWD_003195"
     CDS             complement(3252..4172)
                     /gene="speB"
                     /locus_tag="ACHXWD_003195"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR7622035.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4310..6286)
                     /gene="speA"
                     /locus_tag="ACHXWD_003196"
     CDS             complement(4310..6286)
                     /gene="speA"
                     /locus_tag="ACHXWD_003196"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR7622036.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6295..6426)
                     /gene="yqgB"
                     /locus_tag="ACHXWD_003197"
     CDS             complement(6295..6426)
                     /gene="yqgB"
                     /locus_tag="ACHXWD_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR7622037.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6520..6663)
                     /gene="yqgG"
                     /locus_tag="ACHXWD_003198"
     CDS             complement(6520..6663)
                     /gene="yqgG"
                     /locus_tag="ACHXWD_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR7622038.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6774..7025)
                     /gene="yqgD"
                     /locus_tag="ACHXWD_003199"
     CDS             complement(6774..7025)
                     /gene="yqgD"
                     /locus_tag="ACHXWD_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR7622039.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7081..8235
                     /gene="metK"
                     /locus_tag="ACHXWD_003200"
     CDS             7081..8235
                     /gene="metK"
                     /locus_tag="ACHXWD_003200"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR7622040.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2031 a         2279 c         2175 g         1918 t
ORIGIN      
        1 ttatctattg cttgtccgca aacggacata tcaaggtaat aaaaaaggtc gccgaagcga
       61 cctttttacc cgaaatgcta attacagcag ttcttttgct ttcgcaacga cgttgtcgac
      121 ggtgaagccg aactcttcga acagcaactc tgccggagct gactcaccga aggtggtcat
      181 accgatgata gcgccattca ggccaacata cttgtaccag tagtcagcaa tacccgcttc
      241 taccgcaacg cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag ccgcatcctg
      301 cttgtcgaat gcatcggtag acggcatgga aaccacgcgc gccttcacgc cttcggcagt
      361 cagtttttcg taggcggcaa cagccagttc aacttccgaa ccggtcgcga tgaagatcag
      421 ttccggctga ccagcacagt ctttcagcac ataaccaccg cgcgcgatgt ttgccagttg
      481 ctcttcagta cgttcctgct gcgccagatt ctgacgggag aggataagcg cggtcggacc
      541 gtcctgacgc tcaacaccgt atttccacgc gaccgcggat tcaacctggt cacacggacg
      601 ccatgtagac atgttcgggg tcacgcgcag agaagcgacc tgctcaaccg gctggtgagt
      661 cgggccgtct tcgcccagac cgatggagtc gtgggtgtaa accatcacct gacgctgttt
      721 catcagcgca gccatacgta cagcgttacg tgcgtattcg acaaacatca ggaaggtgga
      781 ggtgtacggc aggaagccac cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa
      841 ctcgcgaaca ccgtagtgga tgtagttacc cgcagcatct tcgttgattg ctttagaacc
      901 agaccacagg gtcaggttag acggtgccag gtcagcggag ccgccgagga attccggcaa
      961 cagcgggccg aacgcttcaa tagcattctg agacgcttta cggctggcga ttttcgccgg
     1021 attagcctgc agtttagcga taaactcttt cgctttggcg tcgaagtcag acggcatttc
     1081 gcctttcata cggcgggtaa attcagccgc ttcctgcgga taagctttcg cgtaagccgc
     1141 aaacttctca ttccatgcag attctttcgc ctggcctgct tctttcgcat cccactgcgc
     1201 atagatttca gacgggattt cgaacggtgc gtatttccag cccagctgtt cgcgggtcag
     1261 agcgatttct gcatcaccca gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg
     1321 ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag tcactgcgcg
     1381 cgcttcttct actgcgcgtt tgatggatgc tgcgtcatga ccgtcgatgt cgcgaataac
     1441 gtgccagccg taagcttcga aacgcattgc ggtgtcgtcg gtgaaccagc cttcaacgtg
     1501 accatcaata gagataccgt tgtcatcgta gaacgcaatc agtttaccca gcttcagcgt
     1561 acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt cgcccatgaa
     1621 ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt
     1681 tttttctgca atcgccatac cgactgcgtt ggcaataccc tgacccagcg gaccggtggt
     1741 ggtttccaca ccagcggtat aacctacttc cgggtggccc ggagttttag agtgcagctg
     1801 acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta
     1861 gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag cccaggacgg
     1921 attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt cagccatacc
     1981 cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc tcagcgcacg
     2041 aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga tgatgaaggg
     2101 cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat ttgccaacaa
     2161 tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca gaaatctctg
     2221 gatcatgctc gcatgttgcg caatctactc gcccgtccgc tgcgcttttc cttatactga
     2281 gactgagcgt cgattcacct gcaaacggcg catttttaga ataatcctga ccttgtgcgg
     2341 aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa cggtactgac
     2401 tggttgccag aatatggact ccaacggact gctctcatca ggagcggaag cttttcaggc
     2461 ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc aggagatgga
     2521 cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga caactattgc
     2581 caatgcgcta ggcaacaata tcaacggtca gccggtgaat tacaaagtgt atatggcgaa
     2641 ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg ggctgatgga
     2701 tatgatgaca gataacgaag tcgaagcggt gatcggtcac gagatggggc acgtggcgtt
     2761 aggccatgtg aagaaaggga tgcaggtggc gcttggtaca aatgccgtgc gagtagctgc
     2821 ggcctctgcg ggcgggattg tcggcagttt atctcaatca caacttggtg atctgggcga
     2881 gaaattagtc aattcgcaat tctcccagcg ccaggaagca gaagccgatg attattctta
     2941 cgatcttctg cgccaacgcg gcatcagccc ggcaggtctt gccaccagct ttgaaaaact
     3001 ggcgaaactg gaagaaggcc gccaaagctc aatgtttgac gaccatccgg catccgccga
     3061 acgcgcccag catattcgcg atcgcatgag cgcggatggg attaagtaaa gcctggtggt
     3121 gtcgaacgta ggtcagataa ggcgttcacg ccgcatctga catcaatggc acatttgtta
     3181 ccctgtgcgc attgccggat gcgatgctga ctgcatctta tccggcctac gggtggcatc
     3241 gcatctggtg cttactcgcc ctttttcgcc gcttggatat acagcatctc cagcgccagc
     3301 gtcgccgcag ccagagcggt gatttccgac tgatcgtatg ccggagccac ttccactacg
     3361 tccatcccaa caatgttgag atctttcagg ccgcgtacca gtttaatagc gcgatcggag
     3421 gtcaggccgc caatcactgg cgtaccggtg cctggtgcaa aagcaggatc caggcagtcg
     3481 atatcaaaag tcaggtaaac cggcatatca cccacaatct gtttcacttg ggcgataacg
     3541 tcatccacgc tgcgatcgtt cacctggcag gcgtccagca cggtaaagcc gttgtcttta
     3601 tcaaactcgg tacgaatacc aatctgcacg gaatgattcg ggtcgatcag accttctttc
     3661 ggcgcggtat agaacatagt gccgtggtca aattcacaac cgttcgcata ggtatcggtg
     3721 tgggcgtcaa agtgtaccag cgccattttg ccgaaatgct tcgcatgagc acgcagcagc
     3781 ggcagcgtaa caaagtggtc accaccgaag gtgagcatac gcttaccggc agccagcagc
     3841 ttctcggcgt gcgcctgcag cttttcgctc atctcacggg catcgccaaa ggcatatacc
     3901 agatcgccgc agtccacgac gttcagacgc tcacgcatgt cgaaattcca cgggaagcgg
     3961 ttgtgttccc aggccagatt cgtcgaaacc tgacggatcg ctgccggacc gtggcgacca
     4021 cccgcacgac cagaagtggc catatcgaac ggcacgccag taatcaccca gtctgcatcg
     4081 ctgtcatacg gctggaagtt catcggcagg cgtaaaaaac caaaggcatt ggaaaccagt
     4141 gagttatcgt attgatgacc taaggtgctc atggatggac tcctattaca aagtcgatat
     4201 aaaaaaaccc ttccgcgtcg ttaggcccga cgaggaaggg ttggatttgt cacaataaat
     4261 tgtggcggat tatcaccgct aaattaagcg gattcaagta acacaggact tactcatctt
     4321 caagataagt ataaccgtac aaacctgcct cgaactcttc aaggaactgt tgttgcagtt
     4381 cagcatcaag atcggttttc ttcacttgat cgcggaactg ggttaacagc gttttcggat
     4441 cgagctgtac atattgcagc atgtccgcca cggtatcgcc ttcgtcagac agttctactt
     4501 ctacgctacc gtcagggaac acgaacacgt caaccgcttc ggtatcaccg aacaggttgt
     4561 gcatattgcc aaggatctcc tgatatgcgc cgaccataaa gaaaccgagc atcggcggat
     4621 tctctggatc gtactccggc attggcattg tcgtggcaat accatcacca tcaatatagt
     4681 ggtcgatagc accgtcagag tcacaggtaa tatccagcag cacagcgcga cgttccggca
     4741 cttgatccag cccttccagc ggcagaaccg ggaacaactg gtcgatcccc catgcgtccg
     4801 gcattgactg gaacagcgag aaattgacgt acattttgtc cgccatacgt tcctgcagtt
     4861 cgtcgattat cggacgatgc gcacggtttt gcggatccag ttgcttctgc acttcatggc
     4921 acatgctcag atagagttgt tctgcccatg cacgttcttg caggctaaag gtgccggaag
     4981 agtagccgat atgaatgtcg tgcagatcca tctggctgtc gtgtaaccat tcacgcaaag
     5041 aacggcgagt tcccggttcg tgcatttcct gccaggtttc ccacatgctt tgcagcgcgc
     5101 gcggcgcatc ttctgcgggc gcggtcggca ccgtatattc gttacgttcc acgccgatga
     5161 tattcgacac cagcacggta tggtgcgcag tcaccgcacg gcccgattcg gtgattaccg
     5221 tcggatgcgg cagaccattt tcttcacacg catcgccaat cgcccagatg atgttgttgg
     5281 cgtattcatt gaggccatag tttacggagc agtcagactg cgaacgggtg ccttcatagt
     5341 ccacgcccag accgccgccg acgtcgaagc actgaatatt gacgcccagc ttgtgcagtt
     5401 ccacatagaa acgcgcggat tcacgaacgc ccgtggcgat atcgcgaata ttcgccatct
     5461 gcgaaccgag gtggaagtgc agtagttgca ggctgtcgag acgcccggct tcacgcaggg
     5521 tttcaaccag ttgcagtacc tgagtcgcag ccaggccgaa cttcgatttt tccccgccgg
     5581 aggactgcca tttacccgag ccctgcgaag ccagacgtgc acgcacgccc agacgaggaa
     5641 cgacattcag acgttctgct tcatccagca caatggcgat ttctgacatc ttctcaatga
     5701 ccagatagac cttgtgcccc atcttctcgc caattaatgc caggcggata tattcgcggt
     5761 ctttataacc gttgcagacg atgacgctac gggtcatgcc agcatgtgcc agtactgcca
     5821 tcaactcggc tttggaaccg gcttccagac ccagcggttc gcccgaatga atcagggact
     5881 caatcacgcg gcggtgctgg ttaactttga tcggataaac aaggaagtaa tcgccgttat
     5941 agccgtagga ttccctcgca cgtttgaacg cggcgttaat ggaacgcaaa cggtgctgca
     6001 ggatctgtgg gaaacagaac agtgcaggca gacgctgacc ctgtgcttca cgagttttca
     6061 ctaactgcgc gagatcgacg cgagcttccg ggacgtccgg gtccgggcac acgctgatgt
     6121 ggcccagctc gttaacgtca tagtagttat tgccccacca ggcaatattg taagtacgca
     6181 gcatcttgct ggcttcctgg gagctcattg caacctcctg catggagcgt agtacaccgt
     6241 gttcgcccgc tgacgaaggc aaacccatag acatgtcgtc agacatagcg aacctcaaat
     6301 tattttatta agtgtaaaac agttaacgac tatcgcagcc tggaactgcg ataacaaccc
     6361 ataagcacat ggattttcca gcagtgaatg ctgacgctcc aactgcgcaa ccggtttctt
     6421 tttcataaca ttattaagca cataaccgaa cgtaagtgtg acagttcggc gaaaccacga
     6481 gaaaactctt gtttttacaa gagcgccctt gttcagtcct cagtaactgt aaccagctct
     6541 tgaatcctga gaagcgccga gatgggtata acatcggcag gtatgcaaag cagagatgca
     6601 gagtgcgggg aacgaatctt caccagaacg gtgagacagg ttaagcagca gacaacggtt
     6661 cattatttcg tatcacctcc acggccgcct gttaagacga acccacaagc caaaactctg
     6721 atttcaaccc ggctggaagt ggcaacacga aagaaacgtc gtgtgctttt tatttaagcc
     6781 gcgcgccgcg ttttataccc cacaatggca gaaaattgca aaagataaat acgcagaatg
     6841 ccggcattgt caggaaaaat ttccagccac gtttttaaca gaatgagaca cgattcaaaa
     6901 aaaagtggaa atcgggtgaa gaattgacct aaaatagcca tccagatgtt aatccatcca
     6961 taccgattaa cactcagact gccagtgttt ttaacctgca gagtcgtggt aggatccgct
     7021 accacagaaa atccacacaa cagtttgagc taaccaaatt ctctttaggt gatattaaat
     7081 atggcaaaac acctttttac gtccgagtcc gtctctgaag ggcatcctga caaaattgct
     7141 gaccaaatct ctgatgccgt tttagacgcg atcctcgaac aggatccgaa agcacgcgtt
     7201 gcttgcgaaa cctacgtaaa aaccggcatg gttttagttg gcggcgaaat caccaccagc
     7261 gcctgggtag acatcgaaga gatcacccgt aacaccgttc gcgaaattgg ctatgtgcat
     7321 tccgacatgg gctttgacgc taactcctgt gcggttctga gcgctatcgg caaacagtct
     7381 cctgacatca accagggcgt tgaccgtgcc gatccgctgg aacagggcgc gggtgaccag
     7441 ggtctgatgt ttggctacgc aactaatgaa accgacgtgc tgatgccagc acctatcacc
     7501 tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta aaaacggcac tctgccgtgg
     7561 ctgcgcccgg acgcgaaaag ccaggtgact ttccagtatg acgacggcaa aatcgttggt
     7621 atcgatgctg tcgtgctttc cactcagcac tctgaagaga tcgaccagaa atcgctgcaa
     7681 gaagcggtaa tggaagagat catcaagccg attctgcccg ctgaatggct gacttctgcc
     7741 accaaattct tcatcaaccc gaccggtcgt tttgttatcg gtggcccgat gggtgactgc
     7801 ggtctgactg gtcgtaaaat tatcgttgat acctacggcg gcatggcacg tcacggtggc
     7861 ggagcattct ctggtaaaga tccatcaaaa gtggaccgtt ccgcagccta cgcagcacgt
     7921 tatgtcgcga aaaacatcgt tgctgctggc ctggccgatc gttgtgaaat tcaggtttcc
     7981 tacgcaatcg gcgtggctga accgacctcc attatggtag aaactttcgg tactgagaaa
     8041 gtgccttctg aacaactgac tctgctggta cgtgagttct tcgacctgcg cccatacggt
     8101 ctgattcaga tgctggatct gctgcacccg atctacaaag aaaccgcagc atacggtcac
     8161 tttggtcgtg aacatttccc gtgggaaaaa accgacaaag cgcagctgct gcgcgatgct
     8221 gccggtctga agtaatcttt cttcacctgc tttcaaaggc cagcctcgcg ctggcctttt
     8281 tcttttacct caccgcggtg gataggcatt cacgccgcat ccggcaaaaa gctgcccgca
     8341 caataacatc attcttcctc aacacgtttt atcgcaaatt atcatcacta ctgaaaccga
     8401 tta
//
LOCUS       DAZSBF010000174         8401 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZSBF010000174 DAZSBF010000000
VERSION     DAZSBF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.177"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHXWD_003201"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="ACHXWD_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR7622041.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(1360..1518)
                     /locus_tag="ACHXWD_003202"
     CDS             complement(1360..1518)
                     /locus_tag="ACHXWD_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622042.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            2089..2238
                     /locus_tag="ACHXWD_003203"
     CDS             2089..2238
                     /locus_tag="ACHXWD_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622043.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="ACHXWD_003204"
     CDS             complement(3005..3445)
                     /locus_tag="ACHXWD_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR7622044.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="ACHXWD_003205"
     CDS             3663..4052
                     /locus_tag="ACHXWD_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622045.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4284
                     /locus_tag="ACHXWD_003206"
                     /pseudo
     CDS             4096..4284
                     /locus_tag="ACHXWD_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHXWD_003207"
     CDS             complement(4251..5450)
                     /gene="tet(A)"
                     /locus_tag="ACHXWD_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR7622046.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHXWD_003208"
     CDS             5556..6206
                     /gene="tetR(A)"
                     /locus_tag="ACHXWD_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR7622047.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(6238..6480)
                     /locus_tag="ACHXWD_003209"
     CDS             complement(6238..6480)
                     /locus_tag="ACHXWD_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR7622048.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6786..7622)
                     /locus_tag="ACHXWD_003210"
     CDS             complement(6786..7622)
                     /locus_tag="ACHXWD_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR7622049.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7622..>8401)
                     /locus_tag="ACHXWD_003211"
                     /note="aph(3'')-Ib"
     CDS             complement(7622..>8401)
                     /locus_tag="ACHXWD_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR7622050.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1889 a         2322 c         2332 g         1858 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaaagcccg cgacgcagcg ccggcaggca gagcaagtag
     4321 agggcagcgc ctgcaatcca tgcccacccg ttccacgttg ttatagaagc cgcatagatc
     4381 gccgtgaaga ggaggggtcc gacgatcgag gtcaggctgg tgagcgccgc cagtgagcct
     4441 tgcagctgcc cctgacgttc ctcatccacc tgcctggaca acattgcttg cagcgccggc
     4501 attccgatgc cacccgaagc aagcaggacc atgatcggga acgccatcca tccccgtgtc
     4561 gcgaaggcaa gcaggatgta gcctgtgccg tcggcaatca ttccgagcat gagtgcccgc
     4621 ctttcgccga gccgggcggc tacagggccg gtgatcattg cctgggcgag tgaatgcaga
     4681 atgccaaatg cggcaagcga aatgccgatc gtggtcgcgt cccagtgaaa gcgatcctcg
     4741 ccgaaaatga cccaaagcgc ggccggcacc tgtccgacaa gttgcatgat gaagaagacc
     4801 gccatcaggg cggcgacgac ggtcatgccc cgggcccacc ggaacgaagc gagcgggttg
     4861 agagcctccc ggcgtaacgg ccggcgttcg cctttgtgcg actccggcaa aaggaaacag
     4921 cccgtcagga aattgaggcc gttcaaggct gccgcggcga agaacggagc gtggggggag
     4981 aaaccgccca tcagcccacc gagcacaggt cccgcgacca tcccgaaccc gaaacaggcg
     5041 ctcatgaagc cgaagtgccg cgcgcgctca tcgccatcag tgatatcggc aatataagcg
     5101 ccggctaccg ccccagtcgc cccggtgatg ccggccacga tccgcccgat atagagaacc
     5161 caaaggaaag gcgccgtcgc catgatggcg tagtcgacag cagcgccggc cagcgagacg
     5221 agcaagaccg gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca
     5281 aattgcatca acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag
     5341 tgaaccagat cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg
     5401 tcgagcgcga cagtgctcag aattacgatc aggggtctgt tgggtttcac gtctggcctc
     5461 cggaccagcc tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg
     5521 gacatattat gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag
     5581 tgatccgtgc cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca
     5641 aactggcgga acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc
     5701 gggcgctgct cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc
     5761 cgagagccga cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg
     5821 cgctgctcgc ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc
     5881 agatggaaac ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg
     5941 acgccgtcaa tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc
     6001 aggccggcga cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc
     6061 cgctgttgcg ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc
     6121 agggactcgc ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg
     6181 aaggaccgag aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta
     6241 cagcagagcc atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca
     6301 gcccgctacg ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc
     6361 ctctctggcg gccttctggc gctcctgctg cggcgtccgc tcgtgggccg tggcgcgggt
     6421 ccgcgcgccg gcctcgtgcg cctggcgctc gcgggcgagg tccagggcgg ccgtcttcac
     6481 gttctgcctt gcgcagatga gatagatcta gcgtggactc aaggctctcg cgaatggctc
     6541 gcgttggaaa ctttcattga cacttgaggg gcaccgcagg gaaattctcg tccttgcgag
     6601 aaccggctat gtcgtgctgc gcatcgagcc tgcgcccttg gcttgtctcg cccctctccg
     6661 cgtcgctacg gggcttccag gggtcgtttg cgggaagggg cggaatccta cgctaaggct
     6721 ttggccagcg atattctccg gtgagattga tgtgttccca ggggatagga gaagtcgctt
     6781 gatatctagt atgacgtctg tcgcacctgc ttgatcgcgg ccgcgatagc tagatcgcgt
     6841 tgctcctctt ctccatccgc gttccaagct gcggaaaggc acccataagc gtacgcctgg
     6901 tcgagcaggc gacgcggatc gacgtccagc gcacgagaga atgcgtccgc catctgtgca
     6961 atgcgtctag gatcgagaca aaggtcgtct ctgtcagccg gatcgtagaa catattggcg
     7021 gcgccaaagc ccacttcacc gaccagaccg acgggatcta tcaccagcca gccgcgactg
     7081 gagaacatga tgttttcatg atgcagatcg ccatgtagcc cacgcagttc cgaggcattg
     7141 ctcatcattt gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt
     7201 tgatcatcgc gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca
     7261 gaaggcaggg gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg
     7321 gtcgcctggt agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat
     7381 tcgagcaaca tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc
     7441 cataccagat agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc
     7501 ttgacgattg caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg
     7561 tccgcaatga gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac
     7621 atcaacccca agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg
     7681 cttcgatccc caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg
     7741 cccagttctc ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc
     7801 cgagccgccc aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt
     7861 tcggcatgca gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca
     7921 ccggtagctc tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt
     7981 ccggtaagaa gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca
     8041 ttcgcgacag cctgcgctca aactgacatt gatcaaccga taggctgtga acagcgccaa
     8101 gttgctgccc cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg
     8161 gtactcccgg aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga
     8221 tgacctcggg gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag
     8281 cgagctcacc gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc
     8341 gaaaaacaaa atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac
     8401 c
//
LOCUS       DAZSBF010000175         8399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZSBF010000175 DAZSBF010000000
VERSION     DAZSBF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.178"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            145..756
                     /gene="ruvA"
                     /locus_tag="ACHXWD_003212"
     CDS             145..756
                     /gene="ruvA"
                     /locus_tag="ACHXWD_003212"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR7622051.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            765..1775
                     /gene="ruvB"
                     /locus_tag="ACHXWD_003213"
     CDS             765..1775
                     /gene="ruvB"
                     /locus_tag="ACHXWD_003213"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR7622052.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1922..2707)
                     /gene="znuB"
                     /locus_tag="ACHXWD_003214"
     CDS             complement(1922..2707)
                     /gene="znuB"
                     /locus_tag="ACHXWD_003214"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR7622053.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2704..3459)
                     /gene="znuC"
                     /locus_tag="ACHXWD_003215"
     CDS             complement(2704..3459)
                     /gene="znuC"
                     /locus_tag="ACHXWD_003215"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR7622054.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3538..4470
                     /gene="znuA"
                     /locus_tag="ACHXWD_003216"
     CDS             3538..4470
                     /gene="znuA"
                     /locus_tag="ACHXWD_003216"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR7622055.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4486..5808
                     /gene="mepM"
                     /locus_tag="ACHXWD_003217"
     CDS             4486..5808
                     /gene="mepM"
                     /locus_tag="ACHXWD_003217"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR7622056.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5928..6899
                     /gene="lpxM"
                     /locus_tag="ACHXWD_003218"
                     /gene_synonym="msbB"
     CDS             5928..6899
                     /gene="lpxM"
                     /locus_tag="ACHXWD_003218"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR7622057.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6987..8399)
                     /locus_tag="ACHXWD_003219"
                     /pseudo
     CDS             complement(6987..8399)
                     /locus_tag="ACHXWD_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2255 a         1985 c         2084 g         2075 t
ORIGIN      
        1 atataacctg tagaataaat tatactgtgc catttttcag ttcatcgaga cacctcgcaa
       61 gttttcttca tccttcgctg gatatctatc cagcattttt ttatcataca gcattatctt
      121 tgattcatta cgcaggagcg tcatgtgata ggcagactca gaggcatcat cattgaaaaa
      181 caacccccgc tggtgttaat tgaagtgggc ggcgtaggct atgaagtgca tatgccgatg
      241 acctgttttt atgaactccc tgaagcgggt caggaagcga tcgttttcac ccactttgtg
      301 gtgcgtgaag acgcgcaact gctgtacggt tttaacaata aacaagagcg cacgttgttt
      361 aaagagttaa tcaaaaccaa cggcgtcggc ccgaagttgg cgctggcgat cctctccgga
      421 atgtcagcgc agcagttcgt taatgccgtt gaacgtgaag aagtgggagc actggtgaaa
      481 ctgccgggca ttggtaaaaa aaccgccgag cgtttaattg tcgaaatgaa agaccgattt
      541 aaaggtttgc acggcgatct ctttacgcca gccgccgatc tggtgctcac gtcgcctgcc
      601 agcccggcaa ccgacgatgc tgaacaggaa gcggttgccg cgctggtggc gctgggctat
      661 aaaccacaag aagcaagccg catggtgagc aaaatcgctc gcactgacgc cagcagtgaa
      721 acattaattc gcgaagccct acgcgccgcg ttatgaggta aaggatgatt gaagcagacc
      781 gtctgatttc tgccggtacc actttgccgg aagatggggc agatcgcgcc attcgcccta
      841 aattactgga agagtatgtt ggtcagccgc aggttcgttc acagatggag attttcatca
      901 aagcagcgaa actgcgcggc gatgccctcg atcatctgtt gatttttggt cctccggggt
      961 tgggtaaaac tacgcttgcc aacattgtcg ccaatgaaat gggcgttaat ttacgcacga
     1021 cttctggtcc ggtgctggaa aaggcgggcg atctggcagc gatgctcact aaccttgaac
     1081 cgcatgacgt gctgtttatt gatgagatcc accgtctatc gccagttgtt gaagaagtgc
     1141 tgtacccggc aatggaagac taccaactgg atatcatgat tggtgaaggt ccggcggcac
     1201 gctccattaa aattgatttg ccgccgttta ccctgattgg tgcaaccacg cgcgcaggtt
     1261 cgctgacatc accgttgcgc gaccgttttg gtattgtgca acgtctggag ttttatcagg
     1321 tgccggatct gcaatatatc gtcagtcgca gcgcacgctt tatggggctt gagatgagtg
     1381 atgacggcgc gctggaagtt gctcgtcgcg ctcgcggtac gccacgtatt gccaaccgtc
     1441 tgctgcgtcg agtgcgtgat ttcgccgaag tgaagcacga tggcaccatc tcggcagata
     1501 tcgctgctca ggcgctggat atgttgaatg tcgatgctga aggtttcgat tatatggacc
     1561 gcaaattgtt gctggcggta atcgataagt tctttggtgg gccggtaggt ctggataacc
     1621 tggcggcagc cattggcgaa gaacgtgaaa ccattgagga tgtgctggag ccttatctga
     1681 ttcagcaagg ctttttgcag cgtacaccgc gtgggcgtat ggcgacgacg cgggcgtgga
     1741 atcactttgg cataacgccg ccagaaatgc cgtaagtcgg attggtcgct cgcaccgcat
     1801 ccgacattgg cagtgtgcct gatgcgacgc tattcgcgtc ttatccggcc taccagagat
     1861 agcgaaccgt aggtcggata aggcgctcgc gccgcatccg acaaatgtgt tcagcgacag
     1921 attagctggc ctgctttttc atcatactga taataaataa cagtgcagca catagcacta
     1981 ccgacggacc cgccggcgta tcgtaaaacg cggaaaaggt taaaccgcca gttactgcca
     2041 ccatccccac caaaacagcg acaccagcca tctgttccgg cgtacgggca aagcgacgcg
     2101 cagtagctgc aggaataatc agcaacgaag taataatcaa cgcaccgaca aatttcatcg
     2161 ctacaccaat cgtcaatgcc gtcaccagca tcaacaacaa tttcacgcgc tgtaatttca
     2221 caccatcaac aaacgccaga tccgggctaa tcgtcatcga cagcaaattg cgccattgcc
     2281 agaacaaaat agccaccacg atgaccacgc caatcgcaat agagatgaga tcttctggcg
     2341 tcactgccag caaatcaccg aacaggtaag ccatcaaatc aacacgaata ttagacatca
     2401 gactaacgac caccaggccc agcgacaggg cactgtgcgc cataatccct aataacgtgt
     2461 cgatcgccag ctgtggacgc ttctccagcc ataccagacc gcccgccagc agcagcgtaa
     2521 cggcaatcac cgcatagaat ggattcacgt ccagcaacaa accaaacgcg acaccaagta
     2581 atgaggcatg agccagcgta tcaccgaaat aagacatacg acgccagact acaaacgaac
     2641 ccagcggacc cgcggcacag gcgagcatga tcccggctaa ccaaccggga aataataatt
     2701 caatcatgag cgatcatttc cccgacgcaa aacaattcgt ccctgtaaat cgtgacgatg
     2761 gttatgatga tggcgataga tacccagttg ttcagcacca cgaggaccaa acattgaaat
     2821 aaactccgga tgcagggaaa caacttccgg tgtgccggaa caacaaatgt ggtgattcag
     2881 gcacagcact tcatcggttt ttgccatcac cagatgcaga tcgtgagaaa ccattaaaac
     2941 gccacaatcc agttcacgac gcagttggtc aataaggtca tataacgcca cctggccatt
     3001 tacatccacg ccttgagtgg gttcatccag cactaataat tgcgggcgat ttaacaatgc
     3061 tcgcgctaac agtacacgct gcgtttcgcc accagagagc ttttgcatcg gtgcgttaat
     3121 caggtgcccg gcctggacac gtttcagtgc aggcaaaata tcttctttat gtgtaccagg
     3181 gcgtaagcgt aaaaaacggt ttacggtcag tggcaacgtg gtgtcgagat acagcttctg
     3241 cggtacatag ccgatgcgca gttttccgtt gcgcttgata accccttcat cgggtgttac
     3301 cagcccgagc actacccgta ccagtgtcga cttacctgcg ccatttggcc caagtaaagt
     3361 caaaattttt ccaggtttaa gttccagcga cacatcagag aggacgcggc gttggccaaa
     3421 agaaaccgag acattttcca aggaaaccag acttgtcatg ttaattttag tcttgcagta
     3481 gtcatgaaat gttataatat cacacttctc atattcatta cgatgattgg tcgcattatg
     3541 ttacataaaa aaacgcttct tttcgcagca ttatccgccg ctctctgggg aggtgcaaca
     3601 caggccgcag atgccgccgt tgtcgcttcg cttaaacccg ttgggttcat cgcttctgcc
     3661 attgctgatg gggtaacaga aacagaggtt ttacttcctg acggcgcttc agaacatgat
     3721 tattcactgc gcccatcgga tgtaaaacgc ttacagaacg cggacttagt cgtttgggtt
     3781 ggcccggaga tggaagcgtt tatgcaaaaa ccggtaagca aattaccagg agcgaagcag
     3841 gtaacgattg cgcagcttga agatgtgaaa ccgctactga tgaaaagtat tcacggcgat
     3901 gatgatgatc acgaccacgc ggaaaaaagt gacgaagatc accatcacgg cgatttcaac
     3961 atgcatcttt ggctttcccc agagatagcg cgggctacag cggttgcaat ccatggaaaa
     4021 ttagtggaac ttatgccgca aagtcgagcc aaacttgacg ccaacctgaa ggattttgag
     4081 gcacaattag cctcaaccga aacgcaggtt ggtaacgagc tcgcgccgct caaggggaaa
     4141 ggttatttcg tttttcacga tgcttacggc tatttcgaaa aacagttcgg actgacaccg
     4201 cttggtcatt ttaccgttaa ccctgagatt caacctggcg cgcagcgttt acatgaaata
     4261 agaacacagt tggttgagca aaaagcaacc tgcgtttttg ctgagccaca gttcaggcca
     4321 gcggtcgttg agagcgtcgc acgagggaca tccgttcgta tgggaacgtt ggatcccctg
     4381 gggacgaata tcaaactggg taaaacaagc tattcagaat tcctgagtca attagccaac
     4441 cagtatgcga gctgcctgaa aggagattaa tgaggaagtg attacgtgca acagatagcc
     4501 cgctctgtcg ccctggcgtt taataattta ccgcgaccac accgcgttat gttggggtcg
     4561 ctcaccgttc ttactctggc cgtcgctgtc tggcggccct atgtttatca ccgcgatgcc
     4621 acgccaattg tcaaaaccat tgagctggaa cagaacgaaa ttcgttcgct cttacctgaa
     4681 gccagtgagc cgattgatca agctgcacaa gaagatgaag ccattcccca ggacgaactg
     4741 gatgacaaaa tcgccggtga agcgggcgtg catgaatatg ttgtttccac tggcgatacg
     4801 ctaagcagca ttctcaatca gtatggtatt gatatgggtg atatcaccca actggctgcg
     4861 gccgacaaag aattgcgtaa cctgaaaatc ggtcaacaac tctcctggac attaaccgcg
     4921 gacggcgaac tgcagcgcct cacctgggaa gtgtctcgtc gtgaaacccg aacctatgac
     4981 cgtactgccg ctaacggttt taaaatgacc agcgaaatgc agcaaggaga gtgggttaac
     5041 aatctgctga aaggtaccgt cggggggagc tttgttgcca gcgccagaaa cgccggttta
     5101 accagcgccg aagtgagcgc agtgattaaa gccatgcagt ggcaaatgga tttccgcaaa
     5161 ctgaaaaaag gcgatgaatt tgcggtgtta atgtctcgag aaatgcttga tggtaaacgt
     5221 gagcaaagcc agctgctggg cgtacgtttg cgttcagaag gtaaagatta ttacgctatc
     5281 cgcgctgaag atggcaaatt ctacgatcgt aacggtactg gtctggcgaa aggattcttg
     5341 cgattcccga cggcgaaaca gttccgtatc tcgtctaact ttaacccgcg tcgtactaat
     5401 ccggtgaccg gtcgcgttgc agcacacaga ggtgttgatt tcgccatgcc gcagggtacg
     5461 ccagtgcttt cagtgggtga cggtgaagtg gtggttgcca aacgcagtgg cgcagcaggt
     5521 tattatgtgg ctattcgtca tggtcgcagc tacaccacgc gttatatgca cttgcgcaag
     5581 attctggtga aaccgggaca gaaggtgaaa cgtggcgacc gtatcgcgct ttccggtaat
     5641 accggacgtt caaccgggcc gcatctgcac tatgaagtat ggataaacca gcaggccgta
     5701 aacccgctga cggcaaaact gccgcgtacc gaagggctga ccggctccga tcgtcgcgaa
     5761 ttcctggcgc aggccaaaga gattgtgccg cagctacggt ttgattaatt aacatccatt
     5821 cgcagccggt acgcagtcag tatcggcttt ttttatttgg tgcggggcaa gttgcgccgc
     5881 tatactatca ccagattgat ttttgcctta tccgaaactg gaaaagcatg gaaacgaaaa
     5941 aaaataatag cgaatacatt cctgagtttg ataaatcctt tcgccacccg cgctactggg
     6001 gagcatggct gggtgtagca gcgatggcgg gtatcgcttt aacgccgcca aagttccgtg
     6061 atcccattct ggcacggctg ggacgttttg ccggacgact gggaaaaagc tcacgccgtc
     6121 gtgcgttaat caatctgtcg ctctgctttc cagaacgtag tgaagctgaa cgcgaagcga
     6181 ttgttgatga gatgtttgcc accgcgccgc aagcgatggc aatgatggct gagttggcaa
     6241 tacgcggacc ggagaaaatt cagccgcgcg ttgactggca agggctggag atcatcgaag
     6301 agatgaggcg taataacgag aaagttatct ttctggtgcc gcacggttgg gccgtcgata
     6361 ttcctgccat gctgatggcc tcgcaagggc agaaaatggc agcgatgttc cataatcagg
     6421 gcaacccggt ttttgattat gtctggaaca cggtgcgtcg tcgctttggc ggtcgtctgc
     6481 atgcgagaaa tgacggtatt aaaccattca tccagtcggt acgtcagggg tactggggat
     6541 attatttacc cgatcaggat catggcccag agcacagcga atttgtggat ttctttgcca
     6601 cctataaagc gacgttgccc gcgattggtc gtttgatgaa agtgtgccgt gcgcgcgttg
     6661 ttccgctgtt tccgatttat gatggcaaga cgcaccgtct gacgattcag gtgcgcccac
     6721 cgatggatga tctgttagag gcggatgatc atacgattgc gcggcggatg aatgaagaag
     6781 tcgagatttt tgttggtccg cgaccagaac aatacacctg gatattaaaa ttgctgaaaa
     6841 ctcgcaaacc gggcgaaatc caaccgtata agcgtaaaga tctttatcct atcaaataaa
     6901 aaaagcctct caaattattg agaggctgta ctcgataaaa aatctcaatg agtgttccca
     6961 ctattttgag tttttaggtt agtacgctat tgagttaatc gtagcgtgat aatttcaaaa
     7021 ggtttgaact caagattgca atattgattg caataaaaac tgtcatctaa cttattttct
     7081 tgaagatcga catattgaac gtagtgataa ttacatactc acgaaagcat ggctgaaatt
     7141 tctaatccat gactctcata taatcttacg attataccgt cgccgtcttc agctaatttt
     7201 attgtttcga tgataacgct gttagtatcg atagcaaaaa atgatagtga agggggtaat
     7261 tcacccgggt gcggtttgac agggacgacc tgtaagggat gattcattat ataggcttga
     7321 ttaataacac cacccacacg gtaatctcca tgatgaggaa ataaggagaa tatgaatttt
     7381 tgtttaccaa tatctgcaaa attattaatc aggaaattat cataattgtc tgtcttaaca
     7441 acttccccag gtttatgctg cgaacgcagc aaagttaatt caagcgtctg gtctttgaca
     7501 cgatagccat atttgttttt tgctattaaa gctatgccag tttctcgatc agaaaggtcg
     7561 acccagtgat gtgctggtat ttcatctttg gcaaaagcga acgaatcatc gctatgcgtt
     7621 ggtctgtcaa ttacaccgaa ctgaatttga cattgtgcaa gagatgtttg tactgacacc
     7681 gggaatttta ctttgagaga aacattaggt tcgtgccaat caataaacgt ttggacatca
     7741 acgcgtggac tatcctgtgt cagggtaaag gctaaacgaa gataagagga attgtagtga
     7801 taaatcagcg tcatcgtagt tttaggcccg ctgatcacag ttgtcatttt ttcagcgagt
     7861 aaaacatatg attccccatc acgataattt gaagcaaaat cccaggccgc gaaaaaacct
     7921 gcatcttcgt ggtatgcacg gatctcactg tgcatatttt cagtaatgta ttctttacca
     7981 tatcttttat catatagcga aattaaatca ccgctttctg aaaaaagaag tttaatgtaa
     8041 ttattttcaa tactttgaga ttctgccttc agaccattta cgatcttatt ctttggaaca
     8101 atcacagcat aaccataaga gtttactcgt gctttgagcc agttgttgtt tatattgatc
     8161 cattcgttac gagcaaaaca ggtggtatta aacaaaatgt gcggatcttt ataagaagaa
     8221 gtgtcaattt tacttaataa agtagactgc gcatcagaaa taattttctc ggtttttgct
     8281 tctagtttaa gatattcctt ttcagtttca tgataaactc ttgaaataca tgagccggga
     8341 aggatgtcat ggaattgcaa ggtcagcatc gctttccata tttcatcaaa ctcactgcg
//
LOCUS       DAZSBF010000176         8361 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZSBF010000176 DAZSBF010000000
VERSION     DAZSBF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.179"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(112..188)
                     /locus_tag="ACHXWD_003220"
     tRNA            complement(112..188)
                     /locus_tag="ACHXWD_003220"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(152..154),aa:Asp,seq:gtc)
     gene            complement(321..1052)
                     /gene="dnaQ"
                     /locus_tag="ACHXWD_003221"
     CDS             complement(321..1052)
                     /gene="dnaQ"
                     /locus_tag="ACHXWD_003221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR7622058.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1117..1584
                     /gene="rnhA"
                     /locus_tag="ACHXWD_003222"
     CDS             1117..1584
                     /gene="rnhA"
                     /locus_tag="ACHXWD_003222"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR7622059.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1581..2303)
                     /locus_tag="ACHXWD_003223"
     CDS             complement(1581..2303)
                     /locus_tag="ACHXWD_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR7622060.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2337..3092
                     /gene="gloB"
                     /locus_tag="ACHXWD_003224"
     CDS             2337..3092
                     /gene="gloB"
                     /locus_tag="ACHXWD_003224"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR7622061.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3164..4522
                     /gene="mltD"
                     /locus_tag="ACHXWD_003225"
     CDS             3164..4522
                     /gene="mltD"
                     /locus_tag="ACHXWD_003225"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR7622062.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4571..5194)
                     /locus_tag="ACHXWD_003226"
     CDS             complement(4571..5194)
                     /locus_tag="ACHXWD_003226"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR7622063.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5198..5998)
                     /locus_tag="ACHXWD_003227"
     CDS             complement(5198..5998)
                     /locus_tag="ACHXWD_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR7622064.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6239..7153
                     /gene="yafC"
                     /locus_tag="ACHXWD_003228"
     CDS             6239..7153
                     /gene="yafC"
                     /locus_tag="ACHXWD_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR7622065.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7150..7953)
                     /gene="dkgB"
                     /locus_tag="ACHXWD_003229"
     CDS             complement(7150..7953)
                     /gene="dkgB"
                     /locus_tag="ACHXWD_003229"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR7622066.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8116..8192)
                     /locus_tag="ACHXWD_003230"
     tRNA            complement(8116..8192)
                     /locus_tag="ACHXWD_003230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8156..8158),aa:Asp,seq:gtc)
BASE COUNT         2231 a         2015 c         2023 g         2092 t
ORIGIN      
        1 catttacagc ataacaaaac catagaccga agaaatagcg ggatttaaat gcaaaaaaac
       61 cgcaaaatta aaaatcttgc ggttctctga actcattttc atgagtgaat ttggcggaac
      121 ggacgggact cgaacccgcg cccccctgcg tgacaggcag gtattctaac cgactgaact
      181 accgctccgc gttgtgttcc gttgggaacg aggcgaatat tacggattgc ctcacccttc
      241 gtcaacggtt tttctcatct tttgaatcgt ttgctgcaaa aatcgcccaa gccgctattt
      301 ttagcgcctt ttacaggtat ttatgcccgc cagaggcagc ttccgccctt cttctccacc
      361 agatcaagac gggcttcatg agctgcaagc tcttcatctg tcgcaaaaac aacgcgtaac
      421 ttacttgcct gacgtacaat gcgctgaatt gttgcttcac cttgttgctg ctgtgtctct
      481 ccttccatcg caaaagccat cgacgtttga ccaccggtca tcgccagata aacttccgca
      541 aggatctgtg catcgagtaa tgccccgtgt agcgttcgtt tactgttatc tatttcgtag
      601 cgggcacata acgcatcgag gctgttgcgc ttaccaggaa acattttcct cgccaccgca
      661 aggctatcgg tgaccttaca gaaggtattg gtcttcggaa tatcgcgctt aagcaacgaa
      721 aactcgtagt ccataaagcc gatatcgaac gctgcgttat ggatcaccaa ctccgcgccg
      781 cggatatagt ccatgaactc atcggctact tcggcaaacg tgggcttatc gagcaaaaat
      841 tcatcggcaa taccatgtac gccaaaggct tccggatcca ccagccgatc gggtttgaga
      901 taaacatgga agttattgcc cgtcaggcga cggttcacca cttcaacggc accaatctca
      961 atgatcttgt ggccttcata gtgcgcacca atctggttca taccggtggt ttcggtatcg
     1021 agaacgatct ggcgtgtaat tgcagtgctc atagcggtca tttatgtcag acttatcgtt
     1081 ttacagttcg attcaattac aggaagtcta ccagagatgc ttaaacaggt agaaattttc
     1141 accgatggtt cgtgtctggg caatccagga cctgggggtt acggcgctat tttacgctat
     1201 cgcggacgcg agaaaacctt tagcgcaggc tacacccgca ccaccaacaa ccgtatggag
     1261 ttgatggccg ctattgtcgc gctggaggcg ttaaaagaac attgcgaagt cattttgagt
     1321 accgacagcc agtatgtccg ccagggtatc acccagtgga tccataactg gaaaaaacgt
     1381 ggctggaaaa ccgcagacaa aaaaccagta aaaaatgtcg atctctggca acgtcttgat
     1441 gctgccctag ggcaacatca aatcaaatgg gaatgggtta aaggccatgc cggacacccg
     1501 gaaaacgaac gctgtgatga actggctcgt gctgcggcaa tgaatcccac actggaagat
     1561 acgggctacc aagtcgaagt ttaagcttgt ggtttacgac attgccgggt ggctccaacc
     1621 gcctggcgaa ttcgtggctt gtttttactc tgtttcatcg ggtttagcgt taaaggaata
     1681 gtccgtttcc gggcaacaat aagttgtaag caaccaagcg caggaatatg cgcattcaat
     1741 agttttcctc cgtgtttgtt ccacgggaga acgtggaaac ggctggcgtg cagcacttca
     1801 aaattcaaca aagagagcca gtccagttgc cgcatcagag taaacatccg gctgttatag
     1861 ggcgaggttt ttcgcaatac cggcacaagt ttgcgtaatc ccattaaact aatgggattg
     1921 aagccactaa tcaccagcca gccatcatca atcaataccc gatcggtttc acgcaataaa
     1981 cgatgcggat cggtgcacca cggcaatgta tgtgccagta gacaaacatc aacggattta
     2041 tcggcaaaag gaagatgaag tggatccgcc tgcacctgga cgggcattcc ttgcgcagaa
     2101 acattcactt gatgagaaac cgcgcacgct tcgcaattga tttctgcgct taaattgcca
     2161 atcttaagca gatgaaaacc atacatttta gtgaaccacg ggttgagctg gcgctccagc
     2221 gccttgcgat aaagctctcc ccagggcaaa tcgccccagc gatcaggagc cacgacagtt
     2281 tgagggactc ttgccggttt catcacaacc ttccgtttca cactaagagg taatctatga
     2341 atcttaacag tattcccgcc tttgatgaca attacatctg ggttttgaat gatgaagcag
     2401 gtcgctgcct gattgtcgat cccggagacg cagagccagt attaaacgcc attgccgcca
     2461 ataactggca accggaggcc atatttctca cccaccatca tcacgatcac gttggcggcg
     2521 taaaagaact ggtggaaaag tttccgcaaa ttgtggtgta tggtccacaa gagacacaag
     2581 ataagggaac aacacaggta gtcaaagatg gcgaaactgc cttcgttttg gggcatgaat
     2641 ttagtgtaat tgccacaccg ggtcacactt taggacatat ctgttacttc agtaaacctt
     2701 atctattttg cggcgacacg ctgttttctg gtgggtgtgg taggttgttt gaagggacag
     2761 catcacaaat gtatcaatca cttaataagt taagtgcgtt acctgacgat acattggtat
     2821 gttgtgctca tgaatatacg ttatcaaata tgaagtttgc tttgagtatt cttccgcacg
     2881 atttgtccat aaatgattat tatcgtaaag ttaaggagtt acgggcaaaa aatcaaataa
     2941 cactacccgt aattctgaaa aatgagcggc aaattaatgt ttttttaaga acggaagata
     3001 ttgatttaat taatgtaatt aatgaagaaa cattattgca acaacctgaa gagcgttttg
     3061 catggttaag gtcaaagaaa gataggttct gataaaactt tcttgtcatc ggctccgttc
     3121 gccgttatga tcggtcgtct tttaagcaac tattgacaca cacatgaagg caaaagcgat
     3181 attactcgcc tctgtcctgc tcgtgggttg ccagagtacc ggcaacgttc aacagcacgc
     3241 acagagcctt tctgcagctg gtcaagggga agcagcaaag tttacaagtc aggcacgatg
     3301 gatggacgat gggacgtcta tcgcgccaga tggtgacttg tgggctttca ttggcgacga
     3361 gctaaagatg ggaattccgg aaaatgaccg gattcgcgaa cagaaacaga aatatttacg
     3421 caataagagc tatctccacg atgtaacttt acgggcagag ccgtatatgt actggatagc
     3481 agggcaagtt aaaaaacgta acatgcctat ggaactggta ctactaccca tagtggagag
     3541 cgcttttgat cctcacgcaa cgtctggcgc caatgccgca ggcatctggc agatcattcc
     3601 gagcacgggg cgcaattatg gtttgaaaca gacccgcaat tatgacgcgc gtcgcgatgt
     3661 tgttgcttca acaactgccg cgctgaatat gatgcagcgt ctgaacaaaa tgtttgacgg
     3721 cgactggctt ctgaccgtag cggcttataa cagcggcgaa ggtcgggtca tgaaggcaat
     3781 taaaacgaac aaagcgcgtg ggaaatccac ggacttctgg tcgttaccgt tgccgcagga
     3841 aacgaagcag tacgtgccta aaatgctggc attgagtgat attctcaaaa acagcaagcg
     3901 ttatggcgta cgtctgccaa cgaccgatga aagccgtgct ctggcgcgtg tgcacctgag
     3961 tagcccggtt gaaatggcga aggttgcaga tatggcgggg atttccgtca gcaagctgaa
     4021 gacattcaac gctggcgtga aaggctccac gctgggcgca agtgggccgc agtacgtgat
     4081 ggtgccaaag aagcatgcag atcaactgcg tgaatctctc gcttcaggcg aaattgctgc
     4141 tgtacagtcg acgctggttg ccgacaatac gccgcttaac agccgtgttt acaccgtacg
     4201 ctctggcgac acgctttcaa gcatcgcttc acgtctcggc gtaagcacca aagatttgca
     4261 gcagtggaac aaactgcgcg gatctaagct gaagccaggc caaagtttga cgattggtgc
     4321 aggcagtagc gcacagcggt tggcaaacaa cagcgatagc attacgtatc gtgtgcgcaa
     4381 aggcgattcg ctttcaagca ttgctaaacg ccacggcgtg aacatcaaag atgtgatgcg
     4441 ctggaacagc gacactgcga atctgcaacc aggcgataag ctgacgttgt ttgtgaaaaa
     4501 caacaacatg ccagattcct gacaaaccag ataataaaaa aggcaccgat tcccccggtg
     4561 ccttttttat ttatgccgct ttatgtgctt ctaccatgat gatatcactg gtgaaagagc
     4621 catcagtctg caaggaaaaa tacgttctca cctctgtcga tgcgctctgt tggtaaatgc
     4681 gaatagcgtc tactaacgct tctggcgtac gcattctcac gacccatgaa gaaaattcca
     4741 gcggtaactt atctgtaatt aaattatcaa ctatcagatt ggcttcattg attaacgtca
     4801 accacgcacc gctggcgtag tttcgtacgt gagaggtatc gcgtaatgct tctaccgcct
     4861 gtaaccagat gtcgcgcact gggtgacccg gagacattac atccatcaca atcagcctac
     4921 cgccaggttt caatatccta ttcacttctc gcagtgctgc accaacatca tgccagtgat
     4981 gggcagaata acggctgata acaatatcaa atgcgttatc ggcaaatggc agacttgcgg
     5041 catatccctg gcgggtggtg atatttttca gttgccgggc ttcggcagct tgtgccacga
     5101 catccagcat gtgggcagat aagtcatacg ccaccaccgc gctcacgttt tgcgcagcgc
     5161 caaacgttgg tctgccctgc ggcagacctg acatacctta tttatcaggc ttgccgggac
     5221 tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccagtaca gaagcttcac
     5281 tgacgttcag accacggtag aaaacaaaat cgagcgggcg accaaacgcc cgacggcgct
     5341 gatcatcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga tataacgcgt
     5401 tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg ccgctgtggt
     5461 gagctatctg atcaccaata ggaagtaact gcttactata gacatccacg cccagactga
     5521 agttgacggc gtgtatatta accaccatca acaggcgggt gtcaggtaat ggatagaccg
     5581 tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac gggcagcaat
     5641 acactggatg tgccgccgaa agggtcatta cgccagaagg atgttgtggc agcacgaaag
     5701 cgggtacctg atcggcggca agatagttag cggtcgcaaa ctgtaccaac tctggcgttg
     5761 tttgcgcttc ctgcaataac accagatgtg cgtctttgcc gtaattcttt aataccgaca
     5821 accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata cgctcttcgg
     5881 tacttaacgg ttctccgggt ggtaatgcct ggccgatgct cgcaaaagac cccggcggta
     5941 agatcctctc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt tttcgcactt
     6001 ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc tactgttata
     6061 gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc aagtgcttac
     6121 accggtcaga aatttgctca ttattgagaa cattaaatcg gatgacatat cacgcatcct
     6181 gatggtttaa tgacaaataa attcaaaata tctcatctat tttgcaacag gagcaaaaat
     6241 gaaagccacg tcggaagaac tcgccatttt tgtttcggtc gtcgaaagcg gcagctttag
     6301 ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcgg tgaaaaagct
     6361 ggaaatgaaa cttggcgtta gcctgcttaa tcggaccacg cggcaactta gcctgacgga
     6421 agaaggcgag cgttattttc gtcgcgtaca gtcaattttg caggagatgg cagcggcaga
     6481 atcagaaatt atggagacgc gtaatacacc gcgtggactg ttacggatcg atgccgcaac
     6541 tccagtggtg ctgcactttc tgatgccgtt aattaagcct ttccgtgaac gctatccgga
     6601 agtcacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa gaaaagtgga
     6661 tgtcgcgata cgcgctggta cgttaacgga ctccagctta cgtgccaggc cgttatttaa
     6721 cagttatcga aaaattatcg cctcccccga ttatatttcc cgctacggga agccagaaac
     6781 gatcgacgat ttaaagcaac atgtttgcct gggattcact gaacccgctt ccctcaatac
     6841 ctggccgata gcctgtagtg atggacaatt acatgaggtg aagtacggtt tgtcatccaa
     6901 tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt gtttgtccga
     6961 ctacatgatc gacaaagaaa tcgctcgcgg agaattggtg gagttaatgg cagataaagt
     7021 gttgccagtg gaaatgccat tcagtgccgt ctattacagc gaccgtgcgg taagtacgcg
     7081 catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg gaggagctgt
     7141 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
     7201 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
     7261 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
     7321 agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcaa tacgagcaat
     7381 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
     7441 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
     7501 gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca acgggatcgt
     7561 gaagttggaa ataccgatct cacgtgtcag accttgtttt ttggcctcca gcagcgcctg
     7621 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
     7681 aacataatcg gtacgcaatt tttgcaggct ctcttttaga ctcgggatca atttgtcttt
     7741 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
     7801 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcag tatcaattgc
     7861 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttgagacg
     7921 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
     7981 ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcataaa atgcagagga
     8041 tttttgcgat tctggcaata atagatatac agacgaaaaa aaacctcgag cgttaactcg
     8101 aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
     8161 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
     8221 atgaccggct tactgcttaa tttgatgcct ggcagtttat gaatcacttc gggctgttgc
     8281 ttcgcaacgt tcaaaagctt tcgcttttgt cctactctct tatcgagtag tgaactgcct
     8341 caaaggtcag tgccattctt c
//
LOCUS       DAZSBF010000177         8296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZSBF010000177 DAZSBF010000000
VERSION     DAZSBF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.180"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            500..1771
                     /locus_tag="ACHXWD_003231"
     CDS             500..1771
                     /locus_tag="ACHXWD_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR7622067.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="ACHXWD_003232"
     CDS             complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="ACHXWD_003232"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR7622068.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="ACHXWD_003233"
     CDS             complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="ACHXWD_003233"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR7622069.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="ACHXWD_003234"
     CDS             complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="ACHXWD_003234"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR7622070.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="ACHXWD_003235"
     CDS             complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="ACHXWD_003235"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR7622071.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="ACHXWD_003236"
     CDS             complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="ACHXWD_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR7622072.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7994..>8296)
                     /locus_tag="ACHXWD_003237"
     CDS             complement(7994..>8296)
                     /locus_tag="ACHXWD_003237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622073.1"
                     /translation="VGLIRRASVASGNGTAFIVGCGATALFDQHTTHFPANFAPHISL
                     SINGFVTETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1911 a         2250 c         2097 g         2038 t
ORIGIN      
        1 catgcccagc tctgcgagcg tcatatagcc gcctgtgttg taatgacaac gtttcgcggc
       61 tattcttgag tggtctaggt tcaagattag cccccgttat gttgtcaggt acatacctgc
      121 aacgtgcggg ggttttctct ctccagcaac caatgccacc agggataaag cccccgcaac
      181 attgcgcctc accggaaccc tttcggcttg ccgctgattt tacgattgct ttttactgtc
      241 agcacccggc aatcttcctc attgcgcgat gccttccagc ttaatttctt tatttgcaat
      301 agttgcagtt catatccggg ctgactccag actggtagtg aaatgcgcaa ttttgtagaa
      361 ttgcgcctcc tcgcgacctc cccagtgaat ctgttgcctc attttttaca tactgcgtat
      421 tcgacttctc cacctgttgc gcaagagaaa ctgggtttat tcatttttgc gaggccgact
      481 tctttctgga caggacttta tgcagcacaa cacactatcg aaacacaatc agaaattgcc
      541 gtttacacgc tacgacttcg gctgggtttt attatgcata ggcatggcga ttggtgccgg
      601 aaccgtgctg atgccagtac aaattggctt gaagggaatt tgggtattta ttaccgcagc
      661 gatcattgct tatcctgcca cctgggtagt gcaggacatt tatttaaaaa ccctttctga
      721 aagcgattcc tgtaatgact acaccgatat tatcagtcat tacctgggga agaactgggg
      781 aattttcctc ggggttatct actttttgat gattatccac gggattttta tctactctct
      841 ctccgtggtt ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg atgccgatct
      901 ttcacaatct ctactttata aagtcgctat tttcgccgta ctggtagcga ttgcgtctgg
      961 tggtgaacga ttactgttta agatttccgg gccaatggtg gtggtcaaag tagggattat
     1021 tgtcgtgttc ggttttgcga tgatcccgca ctggaatttc gccaatataa ccgccttccc
     1081 gcaagcctcc gtctttttcc gcgatgtctt gcttaccatt ccattttgct tcttttctgc
     1141 agtatttatt caggtactta acccaatgaa tattgcctat cgtaaacggg aagcggataa
     1201 agtactggca acccggctcg cgctgcgtac ccaccgaatt agttatatca cgctcatcgc
     1261 ggtgatcctg ttttttgcct tttcgtttac cttctcaatt agccacgaag aagccgtttc
     1321 tgcctttgaa caaaatatct cagcactggc gctggccgcg caggtgatcc ctgggcatat
     1381 cattcatatc acctctacgg tgcttaatat ctttgccgta ctgaccgcat tctttggcat
     1441 ttatctcggt ttccacgagg ccattaaagg cattattctc aatctgttaa gccgaattat
     1501 tgataccaag aaaattaact cacgcgtgct gactctggcg atctgcgctt ttatcgtcat
     1561 tacgttgacg atttgggttt cgtttcgtgt atcggtgctg gtgttctttc agttgggaag
     1621 cccgttatat ggtattgtgt cgtgcctcat tccgtttttc ctgatctata aagtcgcaca
     1681 actggaaaaa cttcgcggat ttaaagcctg gctgattctg ctgtacggca ttttgctatg
     1741 cttgtcgcca ctgttgaagc tgattgagta aaccggagcg catggccccg gttttgtgag
     1801 ttaacgctgc ggattttcat cctgatcaac agcaaaacaa gctaccagtt gaccgccgta
     1861 gtcttttagc tgcggctgca actgggtgca ggggccgaag cgccgacgac agcgggcgtt
     1921 gaaggcgcaa cccggcggtg gattcagtgg gcttggtagt tcaccgctga gcttgatgcg
     1981 ctcgcggcga tcgtccgggt tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg
     2041 atggcgcggg ttattgaaga tttggtcttt cgttcccttc tccacgcagc ggcccaggta
     2101 catcaccatc acttcatcag caatgtgctc caccaccgac aggtcgtggg agataaagac
     2161 ataagacagc cccaactcct gctgcaaatc catcatcaga ttcagcacct gcgcgcgcac
     2221 tgaaacatcc agcgcggaaa ccggttcatc ggcaatcacc acatccgggt cgagcatcag
     2281 accacgggcg atggcgatac gctgacgctg accgccggag aacatatgcg gatagcggtc
     2341 atagtgctcg gttttcaggc cgactttcgc catcatcgac agggcttttt cccgacgctg
     2401 ttctttgctt aagctggtgt tgatcagcag cggctcttca agaatttgcc cgactttttt
     2461 acgcggattc agcgaaccgt aagggttctg gaagacgatc tggattttct gccgacgcag
     2521 cttctgcgcc tgcggatcgt gcttaagcag atcctgcccc tggtaataca gctcgccacc
     2581 ggtgggcgtt tcaatcatcg tcagcaaccg accgagggtc gatttaccgc agccagattc
     2641 gcccactact gccagcgttt tgccacgttc aaggttaaac gaaacgccat ccagcgcttt
     2701 aaccagacgt tccggcgcga acatgccttt cttcaccgga taatgttttt tcaggtcgat
     2761 agcctgcaac agcggttgtt gcagggtggc ctcttgcgta ctcatagtgt cggcctcccg
     2821 gcatcatcaa gtgggtaatg gcatttggac tggcgcccgt cagcgagcat attcagcgcc
     2881 ggttcttcag cgcgacatct gtcagtggca taggggcagc gcgggttaag caggcagccg
     2941 ttcgggcggt cgtacttgcc gggaacgaca cctggcaacg acgccagacg ttctttgtcc
     3001 tgagcaaatt ctggcagcgc acgcagcaat gcctgagtat acgggtgacg cggcgcatgg
     3061 aagatggcgt gcgcatcgcc cgtttccacc acctggcctg catacatcac aatgatttta
     3121 tgcgcagctt ccgccaccag cgccagatca tgggtaatta acaccagcgc catgttctct
     3181 ttctgctgta gctccagcaa cagttcgatg atttgcgcct gaatggtcac gtccagcgca
     3241 gtggtcggct catcggcaat cagcagtttt ggccgacagg caatcgccat ggcgatcatc
     3301 acgcgctggc tcatgccgcc ggaaagctga tgcgggtaaa catccagacg cgatgccgga
     3361 tcgggaatac cgacctgatt cagcaggtca atcgcccgct gacggcgagt gctcttatta
     3421 ccgccctgat gcaccttaat cgcttccatg atctggaaac ccacggtgta gcacgggtta
     3481 aggctggtca tcgggtcctg gaagatcatc gccacttcgg cacccaccag gttgcggcgc
     3541 tctttttctg atatacgctg caaatcctga ccgttaaaca ccagcttttc cgccattacg
     3601 cggcccggat aatcaatcag ccccataatc gccagtgaac tgaccgactt accggagcca
     3661 gactcaccca caatcccgac cacttcgccc tgttttacgc tgtagctgat gcggtctacg
     3721 gcgcggaacg gtgcgctttc gtcgccgaaa tgcaccgata atttatctac atttaataac
     3781 gccatctcga acctcttact gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc
     3841 catcaggtta aatgccagca ccgtcagcag gatcgccaga cccgggaagg tcacgaccca
     3901 ccaggcgctt tgcgcgaact gcaacacgtc ggagagcatg gtgccccact ctggtgttgg
     3961 cggctgcgct cccatgccga ggaagccaag ggcggccata tcgagaatgg cgttagagaa
     4021 accgagcgac gcctgaacaa tcagcggcgc caggcagttc gggaagatgt taataaacat
     4081 ctgacgcatc gccccggcac ctgccacgcg agacgcggta acgtaatcgc gattcacctc
     4141 caccagcacg gcggcgcggg ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc
     4201 cagcgcggcg ttaccaatcg acgggccgaa aatcgccacc agcaccagtg ccaacagcag
     4261 acttggcagc gccagcatga tatcgaccac gcgcatgatg atgttatcga ccaggccgcc
     4321 gaagtaaccg gcgatcaggc cgagaataac gcccataatc agcgataaca ccaccaccag
     4381 acagccaacc agcagcgaca agcgcgcgcc gtacatcagc cgcgacagca catcacggcc
     4441 tacgtcatcg gtgcccagca agtgcgccat gctgccgcct tcctgccagg ctggcggggc
     4501 gagcagcgca tcgcggaact gatccgccgg gttataaggc gcaatccagt tggcaaagat
     4561 cgtgataaac agcacgatga cgatgtaaac cagcccgaca accgcgcctt tgttgcgttt
     4621 aaaatagtgc cagaactcct gtaacggggt catcggcacc ggtgcgctaa tcactttatt
     4681 ttcagtaacc tgtgacatga tgtcccctta cttcttatga cgaatacgcg ggttcaccac
     4741 gccgtacagc agatcgacca gcaggttgac gaggataatc atcgtcgcca ccagcaatac
     4801 gccgccctgc actaccggat agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag
     4861 accgggccac gagaagatgg tttcggtcag aatcgccccc gccagcaatg ttcccacctg
     4921 caggccgata acggtcacta ccggcagcat cgcgttacgc agcgcatgga cgataatcac
     4981 ccgcatgcgg gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc
     5041 cagcatcgaa gagcgcgtca tacgaacgat aaccgccagc gggatagtac ccagcacaat
     5101 agccggtaag atcatatggg caacggcgtc gataaagttg ccatcttcac cccagatggc
     5161 ggtgtcgatc agcataaaac cggttaacgg attggagtca tcgaggaaca ccatatcgct
     5221 cacgcgaccg gagacgggcg tcaggttcca gtgcaccgaa accagcatga tcagcatcat
     5281 gccccaccag aagatcggca tcgagtagcc ggtcagcgcc aggccaactg cagtgtgatc
     5341 gaaaatggaa ccgcgtttaa ccgcagccag cacaccaacc ggaataccga ctgccgtagc
     5401 aaaaatcatc gcgcagacgc caagttccag cgtggcctga aagcgcggca cgaactcttc
     5461 ccatactgga attcggcttt tcattgaaat gcctagatcg ccgtgcatta cgccccaaat
     5521 gtaatggaga tactgctgcc acatcggttt atctaagccg agttcagcca gcagctgcgc
     5581 gtgacgctca ggggagatcc cgcgttcgcc tgccatgatc atcaccggat cgcccgggat
     5641 catgtggaca aaggcaaatg tgagaagggt aataccgata aacgtgggga tgacgagtcc
     5701 caaacgtcgg agaataaact gcaacataac ccggattctc tatagtgacc cgcagcctgg
     5761 aactgcgtgt ctgtattgct cacaaatcaa ttcccgccct tatcggacag ggaatgaagt
     5821 attgctgccg gatggcgctg cacgcagagg cacatcgcca ccgggtatac agattgttga
     5881 caaagtgcgc tttgtttatg ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg
     5941 tgcaaattca atacattgca gaattttcgt aggcctgata agcggagcac atcaggcagt
     6001 tttgcctttg ccatcagtct tgtatggctt ttaattattc gatagagacg ttttcgaagt
     6061 gatgtttgcc taatggatca accacatagc ctttgacttc tttgcgtacc ggttcaaaca
     6121 cggtggagtg agcgatgatc agtgccggag tctgatcgtg catcaccacc tgcgcttgct
     6181 tgtacagttc aacgcgtttg ttatggtcgt cggtagcacg cgccggttga atcagatctt
     6241 caaacggttt gtagcaccat tttgagtagt tagaaccttg ttcagaggcg gcgcagctga
     6301 acagggtggc gaagaagtta tccgggtccc cgttatcgcc agtccagccc atcattaccg
     6361 tctgatgttc gccatctttc gcacgcttga ggtactcacc ccattcgtag gtgacgattt
     6421 tggcctgcac gcccactttc gcccagtctg cctgaatcat ctccgccatg cggcgagcgt
     6481 tcgggttata cggacgttgt accggcatcg cccacaggtc gatggagaaa cctttttcca
     6541 gacccgcttc tttcagcaag gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt
     6601 cgtcgttata gccccacatg gttggcggga tcaggttttt cgctgataca cccgcgccct
     6661 gataaaccgc tttgatgatc gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt
     6721 tcacgtcatc cagcggtttt ttctgcacgt tataggagag gtagccgacg ttcagccccg
     6781 gcatttccat caggttgatg gatttatcct gcttcatgcg agcgatgtct gccgggttcg
     6841 ggtacggcat cacctggcat tcattcttct gcaatttcgc gtaacgcacg gaagcatcag
     6901 gggtaataga gaaaaccagc gtatcgatct gcggtttggt gccccagtag ccatcaaacg
     6961 ctttgtagcg gatacgggaa tctttttggt actgctgtaa ctggaacgga ccggttccga
     7021 ttgggttgag gtccagtttt tccggtgtac cggctttcat catcgcatca gcatattctt
     7081 ttgacagaat agaggcgaag tccattgcca gatcagcgag gaacggcgct tccgggcgag
     7141 tcagtacgaa ctgaactgtg ttgtcgtcca cctttttcac ttcactgatc agctccggca
     7201 agcccatgcc ttcgaagtat tcgtagctgc cgccagagac tttatggtac gggttttgcg
     7261 cgtttttctg acggtcgaac gagaacacca cgtcatcggc gttcagttca cgcgtcggtt
     7321 tgaattcttt attgtcgtgc cacttcacac ctttacgcag atggaaggta taggttttac
     7381 cgtcttcgct gacttcccac ttttcagcga ggcccgggat cacttcagtg gtgccgattt
     7441 taaattcaac caggcggtta taaagcggta cggaagaggc gtcataggtg gtgccggagg
     7501 taaacagctg cgggttaaac ccttccggag atccttctga gcaataaacc agagttttag
     7561 cctgaacact tgctgcgacg gtcatagcca ccaggctgag accaagcttc agcatccctg
     7621 actttttcaa ggaaatacgc attattctgc tccaattgtg atgtttgttg ttttaaccct
     7681 ttgcagtggg ttgtcgctgc ctgacctttt ttgttttttg cccggtcggg tcaacgttat
     7741 gaggtgggga tgccgtacta attaacatca gtgtaacagt gcggatcctc cataaaatgc
     7801 ccctcgtgac ctacaatctg tcaacagaat gtgaaaacgt caatacagcc aaccgggatt
     7861 tacaccaacg gtgagaatcc acaaacaaag attaaaaaaa cttcaaacag ctatttgcag
     7921 cagagaaatt tgtgctactc caacataacc agaacaatca gcttaatatt tagcaacatt
     7981 tggtgattaa gttttatgca tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc
     8041 gattaatacc atgaacgcta aaacgcacag cggaaaatgc cagcgccagc cataagtaac
     8101 gcaaaaaaag atttaagcaa atataaaaaa agacaatggt ttctgtcaca aatccgttaa
     8161 tggatagtga gatatggggc gcaaagttgg caggaaaatg tgtggtgtgt tggtcgaata
     8221 aggcggtcgc gccgcatccg acaataaatg cggtgccgtt gcctgatgcg acgctggcgc
     8281 gtcttatcag gcctac
//
LOCUS       DAZSBF010000178         8273 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZSBF010000178 DAZSBF010000000
VERSION     DAZSBF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.181"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            52..936
                     /locus_tag="ACHXWD_003238"
     CDS             52..936
                     /locus_tag="ACHXWD_003238"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR7622074.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHXWD_003239"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="ACHXWD_003239"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR7622075.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHXWD_003240"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="ACHXWD_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR7622076.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHXWD_003241"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="ACHXWD_003241"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR7622077.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="ACHXWD_003242"
     CDS             5160..6443
                     /locus_tag="ACHXWD_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR7622078.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="ACHXWD_003243"
     CDS             6590..8065
                     /locus_tag="ACHXWD_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR7622079.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1915 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actgaaccga aggagtgcca tttatcatga aattgcacca tagaatgctc cgg
//
LOCUS       DAZSBF010000179         8262 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZSBF010000179 DAZSBF010000000
VERSION     DAZSBF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.182"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(436..1632)
                     /gene="hemY"
                     /locus_tag="ACHXWD_003244"
     CDS             complement(436..1632)
                     /gene="hemY"
                     /locus_tag="ACHXWD_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR7622080.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1635..2846)
                     /gene="hemX"
                     /locus_tag="ACHXWD_003245"
     CDS             complement(1635..2846)
                     /gene="hemX"
                     /locus_tag="ACHXWD_003245"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR7622081.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(2868..3608)
                     /gene="hemD"
                     /locus_tag="ACHXWD_003246"
     CDS             complement(2868..3608)
                     /gene="hemD"
                     /locus_tag="ACHXWD_003246"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR7622082.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3605..4546)
                     /gene="hemC"
                     /locus_tag="ACHXWD_003247"
     CDS             complement(3605..4546)
                     /gene="hemC"
                     /locus_tag="ACHXWD_003247"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR7622083.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4933..7479
                     /gene="cyaA"
                     /locus_tag="ACHXWD_003248"
     CDS             4933..7479
                     /gene="cyaA"
                     /locus_tag="ACHXWD_003248"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR7622084.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7519..7839)
                     /gene="cyaY"
                     /locus_tag="ACHXWD_003249"
     CDS             complement(7519..7839)
                     /gene="cyaY"
                     /locus_tag="ACHXWD_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR7622085.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8001..8143)
                     /locus_tag="ACHXWD_003250"
                     /pseudo
     CDS             complement(8001..8143)
                     /locus_tag="ACHXWD_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1846 a         2173 c         2144 g         2099 t
ORIGIN      
        1 ttagttttaa cggcaattag tatatgtgtt attaattgaa tgaattttat cattcataat
       61 aagtatgtgt aggatcaagc tcaggttaaa tattcactca ggaagcaaag aggattacag
      121 aattatctca taacaagtgt taagggatgt tatttcccga ttctctgtgg cataataaac
      181 gggtagatgc tcattccatc tcttatgttc gccttagtgc ctcataaact ccggaatgac
      241 gcagagccgt ttacggtgct tatcgtccac tgacagatgt cgcttatgcc tcatcagaca
      301 ccatggacac aacgttgagt gaagcaccca ctgttgtcat acagacctgt tttaacgcct
      361 gctccgtaat aagagcaggc gtttttttat gcatcagaaa ggtcccggag gtgcttgcct
      421 ccgggtgaga aggaactact gtggtgggtt gttctgcaac gttaacatca aaccgtcgcg
      481 acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc agcgcgtcgg caagccatgc
      541 gtaatcgtag gcgtccggac gttgtttcag cgctgcgcgg aaggcgagcg atgcttcctg
      601 ccattctccg tgcttcatca gtgactggcc cagtgtgctc cacaacagcg ggcgatcgcc
      661 gacgtttttg atctgctggc gcagcacttt ttccagctgt tccggattgt tggttttcag
      721 tcgaggaatc ggcagcagta ggcgatcgtc gtactggcgt ttcaggccat cgatgataat
      781 ttgctgggca gtatcatgat cgtcacattc aataagatgt tccgccattg ccacctgcaa
      841 cgctacctga tgacgcgttt tccggctttg gtttttccac cagttacgca aaccttcact
      901 accgttatcg gcacgcgcct gatccatcag gccaatccat gcctgttgtt ccagcattgc
      961 acgatgttct tcatcaccaa catgggcttt cgccattgat gggatgatat ccagcagaga
     1021 actccatgca cctgtgcgga tatacgcctg ttccgccaga cgtaatactt ccggatggcg
     1081 tggcgtaact tccagcagct tatccacgcc gtggcgtgca gcatggtttt cattacgggc
     1141 cagttgcaga cgtacacggg tgatttctac cggaatggtg tcattgccgg ccagctctgc
     1201 tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca cgttgttgcg cggcttcggc
     1261 agccagtaga tagttcacca ccggttgttc cgcgtgatcg gcatttttcg ccatcagctt
     1321 ttcaacttgc tgataatcgc cttccgccag tttcagcagc gcctgttcgg tctgcttacg
     1381 ggcacggcga cgcttacgtc cgacaaacca cccacgggtg tgtgcgcctg tgcggaagat
     1441 ccgccgcagt agccactcaa tggcaaacag cactaccatc gccagaatca aaataatcgc
     1501 caggcccgtg acgctggttt cgatattgta gttgtcagtc tggatcagca cgtaaccctg
     1561 atgaccggca atcatcgggc caaccactat ccccgcaatc agcaaaacaa agagtaataa
     1621 cacttttagc atggttattc tccttgcggc gcggctgccg gagtatcacc ttgcggtgca
     1681 ggtgcaggtg caggtgcagg tgcaggtgca ggtgcaggtt tagcttccgt tgtccccgct
     1741 gccggttgtg ccagcaggtt acgcacgcgg gtctgcatca gtttttccag catcgcctgg
     1801 ctttgcaggg tttccggaag atccatcgag atattttgct ggcttaactg gtccacctcg
     1861 tcgaggaacg ctttggtggt ggcatcatca gtatcgtagt aagcacgtac ccaggtggag
     1921 acgttctcca gcgcctggcg ataagtctct tcctgatgac gcggtacagc ttgtgctgcg
     1981 accagcaggc gagagcgaat attttcgcgc agatagatat cctgatttgg cgctaacagc
     2041 ggtacggcgg tgtcatcacg acggcgaatc gtaatgaagt tgtccataaa gttctgccag
     2101 cttttttgca gattgatacg ccattcgctg atggaactgg aaagctcttc accgtctgaa
     2161 tccatcggcg aaccatcgct gtcattatcg gccagacgca ggttatctac ctgatttgaa
     2221 agctgattaa gcttaaggat gatgccgtca taatccacct gcgatactgc agaaaggctg
     2281 gcgatatcat cggtaattgc ccgacgaacg gtaatcagac tcgggtcatt catatccgcc
     2341 aggctggcgt ctgcactttt cagcaacgct gcagcggtcg tgacgtcctg atcgctccac
     2401 agcttccgtc cggcgagttt caccagaaaa tcggcctggg ccagcagcca ggttttagca
     2461 tcgctgccgg aaatggtggc gaccttttgc tggacttcat ccagctgttt tgccagcgtt
     2521 tcttgctgac gattcgcctg ttcaagctgc gtagcctgtt gcttaataat gccttccagt
     2581 tcggcttttt ggctctcctg ggctttttgc aatgccgtca gttggttagc cagggcatcg
     2641 ctggtggcgg tctgattgac ggcctgttgt ttaccccagc catacaaacc gacgcccgcc
     2701 gccagagcaa tagcgatagc cgccgcgctg agaatcaatg cggtattgtt cttactcttt
     2761 ttttctgttg cgacaggttg tgacgtggtg tccacggcct ccctagtctc ttcaaccacg
     2821 gcggaggttt tttcttgttc cgtcattatg gcttcctgtt atgagagtta ttgtaatgcc
     2881 cgtaaaagcg catcgttggc agcgttatcg gcgaccttaa tgtcttgcca gcccagttcc
     2941 cgggcgagtt tcgccaaacg ctcactgacg accaatagtc gacagtgtag taaccagtgc
     3001 tcacgatacc attgtgggat cagcgaccag agttgctgca acatttcacc gctggtaaca
     3061 acgaccgtcg tcacctcgcg ggattgccag cgcatcgctt cttctgcacc atcgtaatgg
     3121 attgcgcatc gttgataaca ttcacaaaaa gtgacctcag caccgcgcgc cgtcagggta
     3181 tccccaatta gctcacgacc gccattgcca cgtaatatca gcgcacgttt gcccgcaata
     3241 ttttgtaatt caggtaattg tagcaagact tcgctgattt cccgatcctg cgggtagaga
     3301 atcttctgtc cgcttacggt atgtagtgcc agtgcggtgg tgcgtccaat ggcgaaataa
     3361 ttaggtagtc ggggccattt acgatcttgc tgatgtagct gtgattgggc aaaagcaacc
     3421 gcgtgttgcg agagggcaaa caacagatcg ctctccccca gcgctgccag ttgatcagca
     3481 agttgcggta attgttgacc cggagaaaac tcaatcagcg gaaaatgcca ggccacctgc
     3541 cccagtgtgc gcagacggct cactaactct tctccagcgg gagacgggcg ggtgacaagg
     3601 atactcatgc cggggcgtct ccgttataga cttcagcgag gatctcgcgc gcgccgttat
     3661 tcagtagctc ttctgccagc gaaatcccca tttgtttggc attttgcggc gcaccgcggc
     3721 gttcaccgcg aataatctgc gaaccgtccg gcgcgccgac cagcgcacgc agccagattt
     3781 cgccatcaat aagctcggcg tagctaccaa ttggcacctg acatccgcct tcgagacggg
     3841 tattcatggc gcgttctgcg gtaacgcgca gtgcagtttc gtggtgattc agcgcggcaa
     3901 gcagctcgcg agtgcgcgta tcatcaaggc ggcattcaat accgactgca ccttgtccta
     3961 ccgccggaag agaaatctcg ggtggtaacg cggcgcgaat gcgggactcc agacctaaac
     4021 gttttagtcc ggctacggca agaatgatgg catcgtattc gccgttatct agtttgctca
     4081 ggcgagtgcc gacgttgccg cgcagggagc ggataatcag atccgggcgg cgttcagcca
     4141 gttggcactg gcgacgtaaa ctggacgtcc cgacgatact gcctgctggt aacgcatcca
     4201 gactgtcata gttattggac acaaaggcat cgcgaggatc ttcacgctca caaatagtga
     4261 ccagtcccag accttgcggg aattcaaccg gcacatcttt cattgaatgt acggcgatat
     4321 cggcgcgatt ttcgaggagc gcgacttcca gctcttttac aaataagcct tttccgccta
     4381 ctttcgccag cggcgtatca agaatcacat cgccgcgcgt caccatcggt accagttcaa
     4441 cgaccaggcc cggatggctc gccatcaact tgtctttgac atagtgtgcc tgccagagtg
     4501 caagtgggct ttggcgtgtg gcaattctta aaacattgtc taacatgctt gttaccgtca
     4561 ttatcatccg tggtccatcc taacatcctt gccagagtga tgtcagtgtt gtggtgaaac
     4621 gtagacgcct gcgcaaaccg taaaatgagg tctggcagtg gatcctgaca gacgtttcac
     4681 gctgtcgtaa taaggaattt acagagaata aacggtgcta cacttgtatg tagcgcatct
     4741 ttctttacgg tcaatcagca aggtgttaaa ttgatcacgt tttagaccat tttttcgtcg
     4801 tgaaactaaa aaaaccaggc gcgaaaagtg gtaacggtta cctttgacat acgaaatatc
     4861 ccgaatgccg cgtgttaccg ttgatgttgg cggaatcaca gtcatgacgg gtagcaaatc
     4921 aggcgatacg tcttgtacct ctatattgag actctgaaac agagactgga tgccataaat
     4981 caattgcgtg tggatcgcgc gcttgctgct atggggcctg cattccaaca ggtctacagt
     5041 ctactgccga cattgttgca ctatcaccat ccgctaatgc cgggttacct tgatggtaac
     5101 gttcccaaag gcatttgcct ttacacgcct gatgaaactc aacgccacta cctgaacgag
     5161 cttgaactgt atcgtggaat gtcagtacag gatccaccga aaggtgagct tccaattact
     5221 ggtgtataca ccatgggcag cacctcgtcc gtagggcaaa gttgttcctc tgacctggat
     5281 atctgggtct gtcatcaatc ctggctcgat agcgaagagc gccagttact acaacgtaaa
     5341 tgcagcctgc tggaaagctg ggccgcctcg ctgggtgtgg aagtcagctt cttcctgatt
     5401 gatgaaaacc gctttcgtca taatgaaagc ggcagcctgg ggggcgaaga ttgtggctcc
     5461 acccagcata tactgctgct tgacgaattt tatcgtaccg ccgtgcgtct cgccggtaag
     5521 cgtattctgt ggaatatggt gccgtgcgac gaagaagagc attacgacga ctatgtgatg
     5581 acgctttacg cgcagggcgt gctgacgcca aatgaatggc tggatctcgg tggcttaagc
     5641 tcgctttctg ctgaagagta ctttggtgcc agcctttggc agctctacaa gagtatcgat
     5701 tccccataca aagcggtgct gaaaacactg ctgctggaag cctattcctg ggaatacccg
     5761 aacccacgtc tgctggcgaa agatatcaaa cagcgtttgc acgacggcga gattgtatcg
     5821 tttggcctcg atccatactg catgatgctg gagcgtgtta ctgaatacct gacggcgatt
     5881 gaagatttca cccgtctgga tttagtacgt cgctgcttct atttaaaagt gtgcgaaaaa
     5941 ctcagccgtg aacgcgcctg tgtaggctgg cgtcgcgcag tgttgagcca gttagtgagc
     6001 gagtggggtt gggacgaagc tcgtctggca atgctcgata accgtgctaa ctggaagatt
     6061 gatcaggtgc gtgaggcgca caacgagttg ctcgacgcga tgatgcagag ctaccgtaat
     6121 ctgatccgct ttgcgcgtcg caataacctt agcgtctccg ccagtccgca ggatatcggc
     6181 gtgctgacgc gtaagctgta tgccgcgttt gaagcattac caggtaaagt gacgctggta
     6241 aacccgcaga tttcacccga tctctcggaa ccgaatctga cctttattta tgtgccgccg
     6301 ggccgggcta accgttcagg ttggtatctg tataaccgcg cgccaaatat tgagtcgatc
     6361 atcagccatc agccgctgga atataaccgt tacctgaata aactggtggc gtgggcatgg
     6421 tttaacggtc tgctgacctc gcgcacccgt ttgtatatta aaggtaacgg cattgtcgat
     6481 ttgcctaagt tgcaggagat ggtcgccgac gtgtcgcacc atttcccgct gcgcttacct
     6541 gcaccgacac cgaaggcgct ctacagcccg tgtgagatcc gccatctggc gattatcgtt
     6601 aacctggaat atgacccgac agcggcgttc cgcaatcagg tggtgcattt tgatttccgt
     6661 aagctggatg tcttcagctt tggcgagaat caaaattgcc tggtaggtag cgttgacctg
     6721 ctgtaccgca actcgtggaa cgaagtgcgt acgctgcact tcaacggcga gcaatcgatg
     6781 atcgaagccc tgaaaactat tctcggcaaa atgcatcagg acgccgcacc gccagatagc
     6841 gtggaagtct tctgttatag ccagcatctg cgcgggttaa tccgtactcg cgtgcagcaa
     6901 ctggtttctg agtgtattga actgcgcctt tccagcaccc gccaggaaac cgggcgtttc
     6961 aaggcgctgc gcgtttctgg tcaaacctgg ggcttgttct tcgaacgcct gaatgtatcg
     7021 gtacagaagc tggagaacgc catcgagttt tatggcgcta tttcgcataa caaactgcac
     7081 ggcctgtcag tgcaggttga aaccaatcac gtcaaattac cggcggtggt ggacggcttt
     7141 gctagtgaag ggatcatcca gttcttcttc gaagaaacgc aagacgagaa tggctttaat
     7201 atctacattc ttgacgaaag caaccgggtt gaggtgtatc accactgcga aggcagcaaa
     7261 gaggaactgg tgcgtgacgt cagtcgcttc tactcgtcat cgcacgaccg cttcacctac
     7321 ggctcaagct tcatcaactt caacctgccg cagttctatc agattgtgaa ggttgatggt
     7381 cgtgaacagg tgattccgtt ccgcacaaaa tctatcggta acatgccgcc tgccaatcag
     7441 gatcacgata cgccgctatt acagcaatat ttttcgtgat gaacgtgccg gaaagcgagg
     7501 cttatccggc atgcaatctt agcggaaact gactgtttca cccgcctgct gcgtcgccgc
     7561 ctgttccagc aaatcccaga aggtttcgcc gctgcgatcg caaatccact catcgccttt
     7621 cagatcaaaa tggtagccgc cttgtttggt tgccagccat acctggtgca gcggttcctg
     7681 gcggttgata atgattttgc tgccgttttc aaaggtaatg gtcagcacgc cgccgttgat
     7741 ttcgcagtcg atatcgctgt cgccatccca gtcgtccagg cgttcttcaa tggtcagcca
     7801 gagttgatca gccaggcgat gaaattcact gtcgttcatt gttgtatcct gtttttaagt
     7861 gatggcggca gtatagcggc atggggtcag ggcttcaaag tttgcacctc tgcggctgcg
     7921 ttccggcacg attcatccgt caccggaata atgatgtctc tgtgtagcga aagatttgtc
     7981 tcttcattag ggcgcagtta caccacgcct ttccctgttt ctggtaaaca ttatgatcag
     8041 gtttaccgag cgagcatcct cacgctgacg gaactaaaaa agacaacaaa caaaccacat
     8101 tgcgatagtg catagagcca tcctggcgcg aggtgccgat cacgaaacta ccagcaaaac
     8161 ataaatcccc acgagtaagc gttatactcg cagcatttcc tcacttttca gacttcataa
     8221 agagtcgcta aacgcttgct tttacgtctt ctcctgcgat ga
//
LOCUS       DAZSBF010000180         8216 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZSBF010000180 DAZSBF010000000
VERSION     DAZSBF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.183"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHXWD_003251"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHXWD_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR7622086.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHXWD_003252"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHXWD_003252"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR7622087.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHXWD_003253"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHXWD_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHXWD_003254"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHXWD_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR7622088.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHXWD_003255"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHXWD_003255"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR7622089.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHXWD_003256"
     CDS             complement(6490..6846)
                     /locus_tag="ACHXWD_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR7622090.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHXWD_003257"
     CDS             complement(6850..7266)
                     /locus_tag="ACHXWD_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR7622091.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHXWD_003258"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHXWD_003258"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR7622092.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2040 a         2089 c         2048 g         2039 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcag
//
LOCUS       DAZSBF010000181         8203 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZSBF010000181 DAZSBF010000000
VERSION     DAZSBF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.184"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            44..568
                     /gene="radC"
                     /locus_tag="ACHXWD_003259"
     CDS             44..568
                     /gene="radC"
                     /locus_tag="ACHXWD_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR7622093.1"
                     /translation="MNKLESLPGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYN
                     VRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEV
                     HPREIIREAIKINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVI
                     GRGEYVSFAERGWI"
     gene            785..1021
                     /gene="rpmB"
                     /locus_tag="ACHXWD_003260"
     CDS             785..1021
                     /gene="rpmB"
                     /locus_tag="ACHXWD_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR7622094.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHXWD_003261"
     CDS             1042..1209
                     /gene="rpmG"
                     /locus_tag="ACHXWD_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR7622095.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1307..2116
                     /gene="mutM"
                     /locus_tag="ACHXWD_003262"
     CDS             1307..2116
                     /gene="mutM"
                     /locus_tag="ACHXWD_003262"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR7622096.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHXWD_003263"
     CDS             complement(2155..2634)
                     /gene="coaD"
                     /locus_tag="ACHXWD_003263"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR7622097.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHXWD_003264"
     CDS             complement(2642..3919)
                     /gene="waaA"
                     /locus_tag="ACHXWD_003264"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR7622098.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHXWD_003265"
     CDS             4332..5390
                     /gene="rfaQ"
                     /locus_tag="ACHXWD_003265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR7622099.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5387..6511
                     /locus_tag="ACHXWD_003266"
     CDS             5387..6511
                     /locus_tag="ACHXWD_003266"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR7622100.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHXWD_003267"
     CDS             6504..7301
                     /gene="rfaP"
                     /locus_tag="ACHXWD_003267"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR7622101.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7317..>8203
                     /gene="waaO"
                     /locus_tag="ACHXWD_003268"
                     /gene_synonym="rfaI"
     CDS             7317..>8203
                     /gene="waaO"
                     /locus_tag="ACHXWD_003268"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR7622102.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWK"
BASE COUNT         2111 a         1889 c         2073 g         2130 t
ORIGIN      
        1 ctgcatgaca aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc
       61 attacctggc ttgttaacct ctgaatatga acaatttagt ggcgttcatg gaattggcgt
      121 agcgaaattt gcccagttaa aggggattgc tgaactggcg cggcgttact acaacgtgcg
      181 gatgcgtgaa gaaagccctt tactcagccc ggagatgacg cgggaatttt tacaaagcca
      241 gctcacgggt gaggagcggg agatctttat ggtgatcttt ctcgactcac aacaccgggt
      301 tataacgcat agccgtcttt tttccggcac gctaaaccat gttgaagtcc atcctcggga
      361 aattatccgg gaagcgataa aaataaacgc ctcggcgctg atccttgcac ataatcaccc
      421 ttcgggttgt gctgaaccca gtaaagcgga taaactcatt actgaacgga taataaagag
      481 ttgtcagttc atggatttac gcgtgctcga ccatatcgtg attgggcgtg gagagtatgt
      541 ttcttttgcc gaacgcggct ggatttaacc cgctatgcgc gatccttcgg gatctttgtc
      601 tgttcgggac ttgagcacat cgctgagtca gcgtatacta cgccaccttt gagaatctcg
      661 ggtttggcat ttgggcctgg caatcgagag ttcacataga actgcgatga ccgggctgta
      721 aagcctgacg aggcgccaat accccatacg aagctcgagc taatttgatt tttggagaat
      781 agacatgtcc cgagtctgcc aagttactgg caagcgtccg gtgaccggta acaaccgttc
      841 ccacgcactg aacgcgacta aacgccgttt cctgccgaac ctgcactctc accgtttctg
      901 ggttgagagc gaaaagcgtt ttgtcaccct gcgcgtatct gctaaaggta tgcgtgtaat
      961 cgataaaaaa ggcatcgata cagttctggc tgaactgcgt gcccgtggcg aaaagtacta
     1021 agtacttaga ggaaataaat catggctaaa ggtattcgtg agaaaatcaa gctggtttct
     1081 tctgctggta ctggtcactt ctataccact acgaagaaca aacgtactaa gccggaaaaa
     1141 ctggaactga aaaaattcga tccagttgtt cgccagcacg tgatctacaa agaagcgaaa
     1201 atcaaataat tctcgctttg atgtaacaaa aaacctcgct ccggcggggt tttttgttat
     1261 ctgcttgccc ccatattgat tgcatctgtt cattcctgga gatgctatgc ctgaattacc
     1321 cgaagttgaa accagccgtc gcggcataga accgcatctc gttggtgcaa ccattcttca
     1381 tgcggtggtg cgcaacggac gcttgcgctg gccggtttca gaagagatct accgtttaag
     1441 cgaccaacca gtgcttagcg tgcagcggcg ggctaaatat ctgctgctgg agctgcctga
     1501 gggctggatt atcattcatt tggggatgtc tggcagcctg cgcatccttc cagaagaact
     1561 tccccctgaa aagcatgacc atgtggattt ggtgatgagc aacggcaaag tgctgcgcta
     1621 caccgatccg cgccgctttg gtgcctggct gtggaccaaa gagctggaag ggcacaacgt
     1681 gctggctcat cttggaccgg agccgcttag cgacgatttc aatggtgagt atctgcatca
     1741 gaagtgcgcg aagaaaaaaa cggcgattaa accgtggctg atggataaca agctggtggt
     1801 aggggtaggg aatatctatg ccagcgaatc actgtttgcg gcggggatcc atccggatcg
     1861 gctggcgtca tcactgtcgc tggcagagtg tgaattgtta gctcgggtga ttaaagcggt
     1921 gttgctgcgt tcgattgagc agggtggtac aacgctgaaa gattttctgc aaagtgatgg
     1981 taaaccgggc tatttcgcgc aggaattgca ggtttacgga cgaaaaggtg agccgtgtcg
     2041 ggtgtgcggt acgccgattg tggcgactaa acatgcgcag cgggcaacgt tttattgtcg
     2101 gcagtgccag aagtaattca tacgcgccgg atggcatacc atccggcata aacgctacgc
     2161 taacttcgcc accagcgcct gatggacatt ctccggcagg aaatgggtga catcgccctg
     2221 atggcgcgcc acctctttca ccaacgatga agagataaac gaccactctt tcgacggcat
     2281 cagaaacaca ctttccagtt ccggcattaa gtggcgattc atatgcgcca gctgcatttc
     2341 atattcaaaa tccgccaccg cacgcaggcc acgaatcagc accgtagcgt gttgattacg
     2401 ggcgaagttc gccatcaaat cactaaaccc aaccacttcc acgttcccca gatgcgcggt
     2461 tgcctgctgt gtcagcgcca cacgctcttc cagggtaaac atcggttttt tactggggct
     2521 ggcggcaatc gccagaataa cgtgatcgaa catttgcgtg gcgcgcgtca cgatatcgat
     2581 atgaccattg gtaatgggat cgaaagtacc cggataaatc gcccgttttt gcataacaac
     2641 ctcaatgcgt tttcggtggc aggtaaggtt ccagcagttg aagcagacgc tgtagcgcgc
     2701 cctggttttg atacagtact tcaacggcat gacggccata gaaactacgg taatcggcgt
     2761 cggtgagtaa agaggaaacc tcttttgcaa gcgtagtggc atcggtaacg gtaatcagcc
     2821 cgcttgcctg ctccagccgc gcgcaaatgt ctttgaaatt aaaagtatgc ggccccatca
     2881 ataccggaat agcgtgtgcg gcagcttcca gaggattatg ccctccacgt tcaaccagtg
     2941 aaccgccaac aaaggcgaga tcggcaatgc cgtacagtaa catcaactcg cccatcgtat
     3001 cgccaaccac aacctgcgtg ctggtggagg ggacttcccc tgaagagcgt gtgatatagc
     3061 ttagtccagc ctggcggaca aggttaatcg catccgggaa gcgttctgga tgacggggta
     3121 ccaggatgag caataaattc gggaattgct gtaataatgc ctgatgtgcg gcgatcacca
     3181 cactctcttc accttcgtga gtgctggtgg caatccatac cgggcggtgt ggtgcccact
     3241 ggcggcgcag cgtcacggct ttagcagcca actgcggcgt tacagaaata tcgaatttca
     3301 ggctaccggt aacggttacc tggttatttt ttgcgcccag cgccacaaaa cgtgcaccat
     3361 cttcttcatt ttgcgcagca atcagcgtaa tacgacgcag taagcggcgg acgaatttac
     3421 ccagtttggc ataacctgcg gccgagcggg cagagagtcg cgcgttagcg atcaccagcg
     3481 gaattttacg tttatgtagc gccgcaatca ggttaggcca tagttcggtt tccataatca
     3541 acaccagttt ggggtcgact ttattcagga aacggttgag cgcatcgggc aggtcatacg
     3601 gcagataaac gtgctgaaca tccttcccga aagccgattg tacgcgctcc gaaccggttg
     3661 gcgtcatggt tgtaacggta atcggtaaat caggataacg atggcgcagc gcgcgcacca
     3721 acgggatcgc cgccagagtt tcaccgacgg agacggagtg cagcataatg ccgcctggtt
     3781 ttagcggatg gcggtaaaaa ccgtaacgtt caccccagcg ttttcgatag gccggagcct
     3841 tacgtccgcg cacccagagc cgtatccaga tcagcggctg aataaggtag agaagggcgg
     3901 tgtaaagcaa ttcgagcata gtaaatagct gacttatgga tgtgctgggg attctatgta
     3961 tttagctgtg gctttaccat tacttttccc gtttttgact taaatagcaa tttacccgcc
     4021 aattcttgct gaacatctat actttttttg tatttttatg cgattcattg atttctgctg
     4081 tacgtgcagg aaggaatcgc agagcaacag tgaaagaata tggttgctaa tgattttatt
     4141 tgattgattt ctttattgaa aatcaacaat gtgcttaaaa agcattcggg tcggattgcc
     4201 gcgaaagaag tgatacacta gcgcgtcaat atcagtgcgg tatcagtatt caggtagctg
     4261 ttgagcctgg ggcggtagcg tgctttttct gcttcacctt aatcggataa tctcaacaaa
     4321 aagagttact tgtggataag ccatttcgaa gaattttgct cattaagatg cgttttcatg
     4381 gggatatgtt attaactact cccgtcatta gttcgctgaa aaaaaattac cctgacgcaa
     4441 aaatcgatgt gctgctttat caggacacca ccccgatcct gtctgaaaat ccagagatta
     4501 acgcgctcta cggcataaaa aataaaaaag caaaagcctc agaaaaaatt gccaactttt
     4561 ttcatctcat caaggtatta cgtgccaata agtatgacct tatcgtcaat cttaccgatc
     4621 aatggatggt tgctatactg gttcgcttat taaatgcccg tgtgaaaatt tcccaggatt
     4681 atcatcatcg gcagtctgct ttttggcgta aaagtttcac ccatttagtg ccgttgcagg
     4741 gtggaaatgt ggtggaaagt aacttatccg tgctgacacc attgggactt gattcgttgg
     4801 tgaagcagac aaccatgagt tacccgcctg caagctggaa acgtatgcgt cgcgaacttg
     4861 atcacgctgg tgttggacaa aattatgtgg ttatccaacc tacggcgcgg caaatcttca
     4921 aatgctggga caacgccaag ttttccgctg tgattgatgc cttacatgct cgtggttatg
     4981 aagtcgttct gacgtccggc ccagataaag acgatctggc ctgcgtcaat gaaattgcgc
     5041 agggatgcca gacgccacca gtaacggcgc tggctggaaa ggtgaccttc ccggaacttg
     5101 gtgcgttaat cgatcatgcg cagctgttta ttggcgttga ttccgcaccg gcgcatattg
     5161 ccgctgcagt taatacgccg ctgatatcgc tgtttggtgc gacagaccat attttctggc
     5221 gtccttggtc aaataacatg attcaattct gggcgggaga ttaccgggaa atgccaacgc
     5281 gcgatcagcg tgaccgaaat gagatgtatc tttcggttat tccggcggca gatgtcattg
     5341 ctgctgtcga taaattactg ccctcctcca cgacaggtac gtcgttatga tcgttgcttt
     5401 ttgtttatat aaatattttc cctttggcgg tttgcagcgc gattttatgc gtattgctca
     5461 gacagtcgcc gcccgaggtc atcatgttcg ggtttatacc cagtcgtggg aaggcgaatg
     5521 ccctgatgta tttgaactga tcaaagtgcc ggttaaatcg cataccaatc acgggcgcaa
     5581 tgcggagtat tttgcctggg tgcaaaaaca tttacgcgaa catcccgtcg ataaagtcgt
     5641 tggattcaac aaaatgccgg ggctggacgt ttattatgcc gctgatgttt gttatgccga
     5701 gaaagtcgcg caggaaaaag gctttttcta tcacctgacg tcacgttatc gccattatgc
     5761 cgcctttgag cgggcaacct tcgaacaggg caagccgaca cagctgctga tgctgacaga
     5821 taagcaaatc gccgatttcc agaaacatta tcagactgaa gcggagcgtt ttcatattct
     5881 gccaccgggg atttatcctg atcgtaaata tagccagcag ccagctaata gccgtgaaat
     5941 cttccgtaag aagaatggaa taaccgaaca acaatattta ttgttgcagg tcggttcaga
     6001 cttcacgcgt aaaggtgtcg atcgttccat tgaagcactt gcttcgttac cggattcgct
     6061 gcgccacaac acattgctat atgttgttgg acaggataag ccgcgaaaat ttgaggcgct
     6121 ggcagaaaaa cgcggcgtgc gcagtaatgt tcacttcttc tcggggcgca acgatgtctc
     6181 ggaattaatg gcggcggcgg atttattact gcatcctgcc taccaggaag cagcgggaat
     6241 tgtgctactg gaagcgatta ctgcaggatt accggtacta acaacagccg tttgtggcta
     6301 tgcgcattat attgtcgacg ccaattgcgg cgaggctatt gctgagccat tccgccagga
     6361 aacattgaat gagattttac gcaaagcgtt aacgcaatct tcattgcgcc aggcctgggc
     6421 ggaaaatgcg cgacattatg ctgatacaca agatttatac agtctgccag agaaagcggc
     6481 ggatatcata acgggtggtc tggatggttg aacttaaaga gccgtttgcc acgttatggc
     6541 gcggtaaaga tccttttgag gaagttaaaa ccttgcaggg tgaggttttt cgtgaactgg
     6601 aaactcgccg cactctgcga tttgaaatgg cgggcaaaag ctattttctc aaatggcatc
     6661 gcggcacgac cctgaaagag ataatcaaaa atttactctc attgcggatg ccagtattag
     6721 gcgcagaccg cgaatggaat gcgattcatc gactgcggga tgtcggcgtt gatactatgt
     6781 atggggtggc atttggcgaa aaaggtatta atccgctgag cagaacctcg tttattatta
     6841 ccgaagatct gaccccaacc atcagtctgg aagattactg tgctgactgg gcgaccaacc
     6901 caccagatgt tcgcgtaaag cgtatgctta ttaagcgtgt cgcgacgatg gtgcgcgata
     6961 tgcatgctgt gggcattaac caccgtgact gttatatctg tcatttcctg ctgcacttgc
     7021 ctttttccgg taaggaagag gagttaaaaa tttcggtaat tgacctgcac cgggcgcagc
     7081 ttcgcacgcg cgttccacgt cgttggcgcg ataaagatct tattgggctt tatttttctt
     7141 cgatgaatat cggcctgact cagcgggata tctggcggtt tatgaaagtg tattttgtcg
     7201 ccccgcttaa agacattctc aagcaggaac aaggactgct gtcgcaagca gaagcaaaag
     7261 ccacaaaaat cagggaaaga acgattcgaa aatcgttgta atttccggag agaaatatga
     7321 gtgcccacta ttttaatcca caagagatga tcaataagac aatcatcttc gatgaaaggc
     7381 cagcggcgtc agtggcatca tcattccatg ttgcttatgg cattgataaa aactttcttt
     7441 ttggttgtgg tgtttcaatc acgtcagttt tgttacataa caacgacgtg agttttgttt
     7501 tccacgtttt tattgatgat atccctgaag ccgatatcca gcgtttagcc caattggcga
     7561 aaagctatcg tacctgtatc cagatccatc tggtaaattg tgaacggctt aaggcattac
     7621 cgacgaccaa aaattggtct attgccatgt atttccgttt tgtaattgca gattacttta
     7681 ttgatcaaca agataagatc ctttacctgg atgctgatat cgcctgtcag ggaaacttaa
     7741 agccgctgat aacaatggat cttgccaata acgttgctgc tgttgttact gaacgcgatg
     7801 ctaactggtg gtcgttacgg ggtcaaagtc tgcagtgtaa tgaacttgaa aagggttact
     7861 ttaattcagg tgtcctgtta attaatacac tagcgtgggc gcaggagtcc gtttctgcta
     7921 aagcgatgtc gatgcttgct gataaagccg tcgtttcccg tttaacctat atggatcaag
     7981 atatacttaa tcttatcctg tcagggaaag ttaaattcat tgatgctaaa tacaatactc
     8041 aatttagttt aaattatgaa ttaaaaaaat catttgtttg tccaattaat gatgaaaccg
     8101 tattaattca ttatgtcggc ccgacaaaac cctggcatta ctgggccggt tatccaagtg
     8161 cgcaaccttt tatcaacgcc aaagaagcat cgccctggaa aaa
//
LOCUS       DAZSBF010000182         8182 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZSBF010000182 DAZSBF010000000
VERSION     DAZSBF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.185"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHXWD_003269"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHXWD_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR7622103.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHXWD_003270"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHXWD_003270"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR7622104.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHXWD_003271"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHXWD_003271"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR7622105.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYGTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHXWD_003272"
     CDS             6679..>8182
                     /locus_tag="ACHXWD_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR7622106.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1978 a         2029 c         2322 g         1853 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact tggcggttta tggcacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 tcacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZSBF010000183         8168 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZSBF010000183 DAZSBF010000000
VERSION     DAZSBF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.186"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            350..1138
                     /gene="phoH"
                     /locus_tag="ACHXWD_003273"
     CDS             350..1138
                     /gene="phoH"
                     /locus_tag="ACHXWD_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR7622107.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            1719..2405
                     /locus_tag="ACHXWD_003274"
     CDS             1719..2405
                     /locus_tag="ACHXWD_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR7622108.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHEWADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(2496..2632)
                     /locus_tag="ACHXWD_003275"
     ncRNA           complement(2496..2632)
                     /ncRNA_class="other"
                     /locus_tag="ACHXWD_003275"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(2677..2764)
                     /locus_tag="ACHXWD_003276"
     tRNA            complement(2677..2764)
                     /locus_tag="ACHXWD_003276"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2728..2730),aa:Ser,seq:gga)
     gene            2998..3936
                     /gene="ghrA"
                     /locus_tag="ACHXWD_003277"
     CDS             2998..3936
                     /gene="ghrA"
                     /locus_tag="ACHXWD_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR7622109.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3991..4728
                     /gene="ycdX"
                     /locus_tag="ACHXWD_003278"
     CDS             3991..4728
                     /gene="ycdX"
                     /locus_tag="ACHXWD_003278"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR7622110.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            4752..5306
                     /gene="ycdY"
                     /locus_tag="ACHXWD_003279"
     CDS             4752..5306
                     /gene="ycdY"
                     /locus_tag="ACHXWD_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR7622111.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            5408..5899
                     /locus_tag="ACHXWD_003280"
     CDS             5408..5899
                     /locus_tag="ACHXWD_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR7622112.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(5964..6797)
                     /gene="csgG"
                     /locus_tag="ACHXWD_003281"
     CDS             complement(5964..6797)
                     /gene="csgG"
                     /locus_tag="ACHXWD_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR7622113.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(6824..7240)
                     /gene="csgF"
                     /locus_tag="ACHXWD_003282"
     CDS             complement(6824..7240)
                     /gene="csgF"
                     /locus_tag="ACHXWD_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR7622114.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(7265..7654)
                     /gene="csgE"
                     /locus_tag="ACHXWD_003283"
     CDS             complement(7265..7654)
                     /gene="csgE"
                     /locus_tag="ACHXWD_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR7622115.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(7659..>8168)
                     /gene="csgD"
                     /locus_tag="ACHXWD_003284"
     CDS             complement(7659..>8168)
                     /gene="csgD"
                     /locus_tag="ACHXWD_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR7622116.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         2035 a         1864 c         2045 g         2224 t
ORIGIN      
        1 cgagatctgt tgtacttatt aagtagcagc ggaagttccc ggcagtgata gtcagtcact
       61 atggagatcg cggatggtaa cgtcctgtac tggacatgtt ttagataatc aacgcgccac
      121 cactcgcgga gttttctcct ccggtagtca tctcgtcact ttgcattttc aacctcatcc
      181 tttcttttca tgtgttaccg acgccgtaaa cggtgcgcgt agccgttttt cggcatttta
      241 tccaaaagca aactatggct tacaaggaag ccaaccctct gatgttcgtg cgcataatcg
      301 cgctgccaac ggcgcgtgtg atgaatacaa acaactcaag gtgctctcta tgggaagaca
      361 aaaagcagtg atcaaagctc gtcgcgaggc aaaacgtgtg ctgagacggg attcgcgcag
      421 ccataagcag cgtgaagaag aatcggtcac ctcgcttgtg cagatgggcg gcgtagaagc
      481 cattggtatg gcccgcgaca gtcgcgatac ttcgcccatc ctcgcgcgaa atgaagcgca
      541 attgcactat ctgaaggcta ttgagagtaa gcagctgata ttcgccacgg gcgaagccgg
      601 gggcggaaaa acttggatca gcgcagcaaa agcggcagag gccctgatac ataaggatgt
      661 cgacaggatt atcgtcaccc gtccagttct gcaagccgat gaagatcttg gcttcttacc
      721 tggagatatc gcagaaaagt ttgctcccta ttttcgcccg gtctacgacg tgctggtccg
      781 gcgcttaggg gcttccttta tgcagtactg cctgcgaccg gaaataggga aggtggaaat
      841 tgcgccgttc gcctatatgc gtggacgtac ctttgaaaat gcagtcgtca ttcttgacga
      901 ggcgcagaat gtgactgccg cgcaaatgaa aatgttttta acccgcctcg gggagaacgt
      961 gacggttatc gtcaacggtg atatcacgca atgcgatttg cctcgcggcg tgtgctccgg
     1021 attaagtgac gcgctggaac gtttcgaaga agatgaaatg gttgggatcg tccggttcgg
     1081 taaagaggac tgcgtacgtt cggcactttg ccaacgtacg ctgcatgcct acagttaagt
     1141 gtgttatcgg tgcagagccc gggcgaaccg ggctttgttt tatttcaaca gattttcact
     1201 tcaggttagc gttcaggggg gcgactttga gcgtgttgat aagctgaact ggagtctggt
     1261 ttagatactc ttaccaggca tttttcgtca tagcatcaga ccagtgtaat actgccagcg
     1321 cggagagttt ttcttccaga agccaaaaaa atgagcatct gcctatcttt tttcggccat
     1381 tcaacctgta caggagatct ggtgccaatt ttgcttatga ggtttaatca acaatgggct
     1441 atactttgac tgactgttta acaccgaata tattcctctg atttattctt tagggataat
     1501 attataaatg taaggttaat cttaattaat tctttgctaa tattgtgtgt tgttattttt
     1561 ttgtggaagg caagccgcaa tggctaaata gggatggttg taataatcaa aacatgatac
     1621 taactatata ttgatcgttg cattgttggc atgtaacacg ctgcagcgag ttacattggt
     1681 aatgaaatat aatcaacatg gataggtgaa aattaattat gagctttatg gcttttgtaa
     1741 cttgtttgac aattgcattt atcgccggtt ttgctgttgc cagaaaattg acactccgaa
     1801 aagttgcctc atcggtaatc atcgctaaat cgcaacccgt cccttcatct gcaacttccc
     1861 ctgccgctaa ggcgtcgaca aggagctata taccgcagaa aacctatcgt gactctcgtt
     1921 ggcaagagcg agatgagtta tttcagggaa tggttgccga aggtatttta ttggaaagaa
     1981 aagagtggtt aatcacgcgt tgtgaaaaaa tgtatttcga taatctggaa aaatggtttg
     2041 gacattattg tcatatccat tgccaggtct ctctggggcg cttgatcaac tttccagaac
     2101 aacctgagtt tagcgaagaa gaaagaaagc gtttctttgc aatttataat ggcatggcaa
     2161 tggattatgt tctggtttca aagaagaaca ataaaatagt ctgtgttatt gagctggatg
     2221 acccgagtca tgaatgggcg gatcgcatta ttcgtgaccg ttggttggat acaattatga
     2281 ctcttgcacg ggtgcctttt gtacgagctt ctgtcactaa attggaggaa attcctccgg
     2341 tctgggagac tcgtgacaaa attcgcccat tagtcgcggc gacaaagagt gaaccggtgc
     2401 attaactaag tatcccggtc ggggcttctc atccccccgg tgtgtgcaat atacgaaaaa
     2461 aagcccgtac cttcgtacga gctcttcttt aaagatggcg gtgagggggg gattgactcg
     2521 cttcgctcgc cctgcgggca gcccgctcac tgcgttcacg gtctgtccaa ctggctgtcg
     2581 ccagttgtcg aaccccggtc ggggcttctc atccccccgg tgtgtgcaat atacgaaaaa
     2641 aaagcccgta ctttcgtacg agctcttctt taaatatggc ggtgaggggg ggattcgaac
     2701 ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac tcggacacct
     2761 caccaaattg tcgttcctgt cttgctggaa cgggcgctaa tttagggaaa tcatgacctg
     2821 aggtcaacaa actttttgaa aaaatcgcgc gtttattcaa acttcaatca atgtgtggtt
     2881 ttaataagcg aaatctgctt tttttgccac cgaccacgga tttgttatgc tggtggcctt
     2941 tgtagatcat aacgataagt gcgaataaat ttcgcacaac gcttttcggg agtcagtatg
     3001 gatatcatct tttatcaccc aacgttcgat acccaatggt ggattgaggc actgcgcaaa
     3061 gctattcctc aggcaagagt cagagcatgg aaaagcggag ataatgactc tgctgattat
     3121 gctttagtct ggcatcctcc tgttgaaatg ctggcagggc gcgatcttaa agcggtgttc
     3181 gcactcgggg ccggtgttga ttctattttg agcaagctac aggcacaccc tgaaatgctg
     3241 aacccttctg ttccactttt tcgcctggaa gataccggta tgggcgagca aatgcaggaa
     3301 tatgctgtca gtcaggtgct gcattggttt cgacgttttg acgattatcg catccagcaa
     3361 aatagttcgc attggcaacc gctgcctgaa tatcatcggg aagattttac catcggcatt
     3421 ttgggcgcag gcgtactggg cagtaaagtt gctcagagtc tgcaaacctg gcgctttccg
     3481 ctgcgttgct ggagtcgaac ccgtaaatcg tggcctggcg tgcaaagctt tgccggacgg
     3541 gaagaactgt ctgcatttct gagccaatgt cgggtattga ttaatttgtt accgaatacc
     3601 cctgaaaccg tcggcattat taatcaacaa ttactcgaaa aattaccgga tggcgcgtat
     3661 ctcctcaacc tggcgcgtgg tgttcatgtt gtggaagatg acctgctcgc ggcgctggat
     3721 agcggcaaag ttaaaggcgc aatgttggat gtttttaatc gtgaaccctt accgcctgaa
     3781 agtccgctct ggcaacatcc acgcgtgacg ataacaccac atgtcgccgc gattacccgt
     3841 cccgctgaag ctgtggagta catttctcgc accattgccc agctcgaaaa aggggagagg
     3901 gtctgcgggc aagtcgaccg cgcacgcggc tactaataaa gcatcaggat tcctgctatc
     3961 cttggcggga attgaataca ggagagagtt atgtatcccg tcgaccttca tatgcatacc
     4021 gttgccagca cacatgcata tagcacattg agtgattaca ttgcccaggc caaacaaaag
     4081 ggcattaaac tttttgcgat caccgatcat ggcccggata tggaagatgc tccgcatcac
     4141 tggcacttca ttaacatgcg tatctggccg cgagtggttg atggggtagg gatcctgcgc
     4201 ggcatcgaag ctaacattaa aaatgttgat ggtgaaattg attgcagcgg taaaatgttt
     4261 gactcgctgg atctgattat tgccggtttt catgagccgg tttttgcacc acatgacaaa
     4321 gcgaccaata cacaagcgat gatcgccact atcgccagcg gcaatgtgca tataataagc
     4381 catcccggaa atcccaaata tgaaatagat gtgaaagccg ttgctgaagc agccgcgaaa
     4441 catcaggtgg cgctggaaat caataattct tcatttttac actcacgtaa gggcagtgaa
     4501 gacaactgtc gtgcggtagc cgcagcggta cgtgatgctg gaggttgggt ggcattaggc
     4561 tcggattctc acaccgcgtt taccatgggg gaatttgaag agtgtcttaa aatcctcgac
     4621 gcggtagatt ttccgccaga gcgcattttg aatgtttctc cgcgccgctt actgaacttc
     4681 cttgaatctc gcggtatggc accgattgcg gaatttgcag acctttaatt actagtaatg
     4741 gaaatatatt aatgaacgag ttttctatcc tctgtcgtgt gctgggttcg ctctattacc
     4801 gccaaccgca agatccttta ctggtgccgc tgtttaccct gattcgtgag gggaaactgg
     4861 ctgcgaactg gccactggag caggatgagt tactgacgcg tttacagaaa agctgtgata
     4921 tggcgcaagt ctctgccgat tacaatgcgt tgtttatcgg cgatgaatgt gcagtgccgc
     4981 catatcgtag cgcatgggtt gaggacgtaa cggaagcgga agtgcgcgct tttctttccg
     5041 aacgagggat gccactggcg gatacgccag ccgatcacat cggcacattg ctgcttgcgg
     5101 cttcctggct ggaagatcag tcaacggaag atgagagcga agcactggaa acactgttca
     5161 gtgagtatct gttaccctgg tgtggtgcgt tcctcggcaa agtggaggcc catgcaacca
     5221 cgcctttctg gcgcaccatg gcaccgctaa cccgtgatgc cattagtgca atgtgggacg
     5281 agctggaaga agattctgaa gagtaaatgt gatcatcgtc actcataact gcaacagata
     5341 atttgttgtt gcataaaatg tgtgctcgat ctcattcatg gccgcgtttt ctgctatcat
     5401 gcgcggcatg aacatacttc tctctattgc aatcacaaca ggcattctct ccggtatctg
     5461 gggatgggtg gctgtttccc tgggcttact aagctgggcg ggcttcttgg gctgtacggc
     5521 ctactttgcc tgcccgcaag gtgggctgaa agggctggcg atctccgctg caaccctgct
     5581 tagtggcgtg gtgtgggcga tggtcattat ttacggtagt gcactggcac cacatctgga
     5641 aattctcggt tatgtcataa ccggtattgt ggcgtttctg atgtgtattc aggccaaaca
     5701 gctgctgctt tcatttgttc cggggacgtt tataggcgca tgcgcgacgt ttgccgggca
     5761 gggtgactgg aaactggtgt taccttcgct ggcgcttggg ctggtatttg gttacgcaat
     5821 gaaaaacagc ggcctgtggc tggcggcgcg tagtgcaaag acggctcacc gtgagcagga
     5881 aatcaaaaat aaagcgtgag gggcactcac gctttcgctt aaacagtaaa atgccggatt
     5941 attaattccg gcatttttat ctgtcaggat tctggtggaa ccgacatatg gcggtattta
     6001 accagaatgt cattctgccg ttctgctttg ttctgcaaat cccacagacc acggtcgata
     6061 ccatcattaa tcaggaaaat gacccctgtt tcgatagccg acatcagaca cagcataacg
     6121 ggttcgtttg aggtgtaacc cacttcccct tccagcaagc gctggtagtc aataaagcgg
     6181 aaaaccccgg cctgaacttc ataggaaagt atcgtcttac tggtgttcac cgaggagagg
     6241 atctcaccgg tactcacatt gacgacacgc aggttcacgg caatctgatc gagctggtat
     6301 tgcgtgtctg caccgatccc aaaatatctt gccccaacac cgcccgattt aacgttgctt
     6361 tcataaccga taatcgaacc ttcaaccatg atatttgccg ccgttaaaga ttgcagcggg
     6421 attcggttat taatggcaac tgtgccgttt tcttgtgccg cacgaataat cttgcgctcg
     6481 ttaagcaggt tttgtaaact ctgacgctcc agcggtataa accagcgaga atctttcaat
     6541 gccgtgacca gcattgccgt ggcgctttgc ggaacagcag tggagaagtt acttgccggg
     6601 tagggtttaa attgcccggt ttcgtcctga atgttgtata ccgaaacaaa gatcttaccc
     6661 gtcggcgctg gcagatgggt caaatctttg tagctctgag ctcgaggcat taatgtcggt
     6721 ctggcggctt ctttaggcgg ggcggttaag catccgctca gtaacatgac ggcaaccaaa
     6781 agaaataagc gctgcatgat tattttcctt atgaagctgg ggcttaaaaa tcggttgagt
     6841 tattttgtaa acccgaaacc tggatggtcg aggtttgtcc ggttttacga tctgtcacgt
     6901 tcaactgcaa ttgaccatcg cggttagcaa tatcaacaat ataatcgttg gtcaccatgc
     6961 ggcccggttt accggtatta atattcgaca gtagcccacc taaaatttgt gactggatgg
     7021 cctgagtaaa gttatctaac gctgagggtg tttcaatacc aaagtcatcg ttatagctcg
     7081 gatctttata agagttttgg gcctgagcgc tatttaataa aaaagcgcca ttatttgggt
     7141 taccaccaaa gtttggatta cggaactgga aagtcatggt tccagcccaa cttaatggcg
     7201 aaataagcat gagtagaact actgcatgtt tgacacgcat tgcagcctcc gaacaatttt
     7261 ttatttagaa ttcatcatgc gccaaatcgc ccgtacttaa taacgcctga tttatctggc
     7321 gacgatttag tgcttcttca gtttgaatca gtgcaaagac gacagttttc tcgaagtctc
     7381 ttttcaacgg aaataaaaaa gtctggaaaa taacgtcctg attgaccgtt atagtgatcc
     7441 agcttcccca tcgtgcactg ggcctttcat taatcgttaa gttacccgta tagtcacttt
     7501 cccatttatc actaaaggct cggtaaaaat catggccaat agatgaaaca gtatggtcag
     7561 ttagcaatcc cgggacttct acctcaacgg cgtgaagatt ccctgcggcg aacagaaatt
     7621 ctgccgccac aatccagcgt aaataacgtt tcatggcttt atcgcctgag gttatcgttt
     7681 gcccaggaaa ccgcttgtgt ccggtttttt acggctatct tcttgaaaag attataaaga
     7741 tgcgttttaa ccgtattttc gctgatgaac aacgaacgag cgatctcgtt attagacgcg
     7801 ccgatacgca gtttattcag gatctctttt tcccgatgag taaggagggc tgattccgtg
     7861 ctgttataac gatagttacc tgaatgcgta atcaagtagc tggcaagctt ttgcgtaaag
     7921 tagcattcgc cgcgcaggac gccttgcaac ccattgacaa cacgttcttg atcctccatg
     7981 gcataaaaaa cgccgttgat atgaggccag ttttcaatgt cgcggtacgg gtaatcttca
     8041 ggcgtattta gcaacaatat tttgatattg ttgtttttcc tgctcaaagt atcctgccaa
     8101 taatggataa gctttttatc cgcttccatc atatccagaa gaataataga gcctgaagat
     8161 atatcgtc
//
LOCUS       DAZSBF010000184         8155 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZSBF010000184 DAZSBF010000000
VERSION     DAZSBF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.187"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..449)
                     /locus_tag="ACHXWD_003285"
     CDS             complement(<1..449)
                     /locus_tag="ACHXWD_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR7622117.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHXWD_003286"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="ACHXWD_003286"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR7622118.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="ACHXWD_003287"
     CDS             complement(1788..2507)
                     /locus_tag="ACHXWD_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR7622119.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="ACHXWD_003288"
     CDS             complement(2691..3017)
                     /locus_tag="ACHXWD_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR7622120.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHXWD_003289"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="ACHXWD_003289"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR7622121.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="ACHXWD_003290"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="ACHXWD_003290"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR7622122.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="ACHXWD_003291"
     CDS             4977..5252
                     /locus_tag="ACHXWD_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR7622123.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="ACHXWD_003292"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="ACHXWD_003292"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR7622124.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="ACHXWD_003293"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="ACHXWD_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR7622125.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8155)
                     /locus_tag="ACHXWD_003294"
     CDS             complement(7903..>8155)
                     /locus_tag="ACHXWD_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR7622126.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2159 c         1987 g         2087 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaatta
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggtcaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcga
//
LOCUS       DAZSBF010000185         8104 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZSBF010000185 DAZSBF010000000
VERSION     DAZSBF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.188"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(101..796)
                     /locus_tag="ACHXWD_003295"
     CDS             complement(101..796)
                     /locus_tag="ACHXWD_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR7622127.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="ACHXWD_003296"
     CDS             complement(789..2216)
                     /locus_tag="ACHXWD_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR7622128.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="ACHXWD_003297"
     CDS             complement(2227..2946)
                     /locus_tag="ACHXWD_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR7622129.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="ACHXWD_003298"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="ACHXWD_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR7622130.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /gene="rclA"
                     /locus_tag="ACHXWD_003299"
     CDS             4554..5879
                     /gene="rclA"
                     /locus_tag="ACHXWD_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR7622131.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            5988..6224
                     /gene="rclB"
                     /locus_tag="ACHXWD_003300"
     CDS             5988..6224
                     /gene="rclB"
                     /locus_tag="ACHXWD_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR7622132.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6236..6829
                     /gene="rclC"
                     /locus_tag="ACHXWD_003301"
     CDS             6236..6829
                     /gene="rclC"
                     /locus_tag="ACHXWD_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR7622133.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(6989..7858)
                     /locus_tag="ACHXWD_003302"
     CDS             complement(6989..7858)
                     /locus_tag="ACHXWD_003302"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR7622134.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2193 a         1982 c         1732 g         2197 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     5941 ctctgttact ctataacttc ttaatcactt cattgatggt attttatatg tttaaaaaat
     6001 ctgttttatt tgcaacacta ttatctggcg ttatggaatt ttccaccaat gcagatgata
     6061 aaataattct gaaacatatc agcgtctcgt cagtatcagc atcaccgaca gttctggagg
     6121 ataccattgc tgatatagcc agaaaatata atgcttcatc ctggaaagtc acatcgatgc
     6181 gaattgataa taattcaacc gcaacagcag tattgtataa ataaggatgt tcacaatgga
     6241 aaaatacctg cacctgttaa gtcggggaga taaaattggc ctgtcattga ttcgtctgag
     6301 tattgcgatt gtttttatgt ggattgggtt attaaagttt gtcccttacg aggcagacag
     6361 cattacacca ttcgtcgcaa acagtccact aatgtcgttc ttttatgaac acccggaaga
     6421 ctataaacag tatctgactc acgaaggcga atacaaacca gaagcaaggg catggcaatc
     6481 ggccaataat acctatggtt tttccaacgg tcttggcgtc gtggaggtga ttattgctct
     6541 gctggttttg gctaatcctg tcaatcgctg gttaggttta ttgggaggac tgatggcata
     6601 taccacgccg ctggtaacac tctcattttt aatcaccacc ccggaggcat gggtacccgc
     6661 attgggtgac gctcatcatg gtttccctta tttatccggt gctggtcgct tggtattgaa
     6721 agatactctg atgctggcag gtgctgtcat gataatggca gattcggcgc gggaaattct
     6781 taaacaacgc agtaatgaat ccagttcaac gttaaaaact gaatattgat aatccgaaca
     6841 ctgtctgttg ttcacttttt caggcgtcag gccccctttc tgatgatgaa gcctgacgcc
     6901 atattaccgg agatatgaca gagtgaagat caatatcact cccctgttaa aagattcttg
     6961 tcgtctcagg taaaatatat gattaatatc aatcatgtat ttttacagca agacaaccac
     7021 gaacaaattc aggattaaaa tgttttaccg actccccaac ataacccaaa tcaagcgaac
     7081 taatttgtgc catttctttc tctgtcagtg agaaatccca gatagcaaaa ttttcttcga
     7141 tacgctgttg atgtgtcgat ttcggaataa cgaccactcc ccgttgaata ttccaacgca
     7201 gaatgacttg agaaatcgat ttttgatggg catctgcaat actctgaagc agattatttt
     7261 caaagggttt atgtcgtcca ccacctaacg gagcccatgc ttcaggctgc acgttataat
     7321 acttcatggt tgccagcgcc tctggttggg caaaataggg atgcaactcg acctggttga
     7381 ccatcggttt aactcttacc gtttcacaaa agttcgccag aacatgagga tagaaattgg
     7441 aaaccccaat tgcttttaat ttgccctctt cataggcatc ttcgagtgca cgccacacac
     7501 tgaaataatc gcgcatagcc tggtgcaata aatagaggtc gaagtactct agtcccgatt
     7561 tttttaatga tgcttcaata cctgctgctg ccgtatcctg attcaacata tcctgccccc
     7621 acaatttaga cgttataaat aactcatctc gggtacataa accttcagat atagcttcac
     7681 gaacggcttc accgacggca tcttcattac cgtataccgc tgcagtgtcg atgagtcgat
     7741 aacccgtgcg gatagcgttc aacactgact gtttgcacac gtttttatcg gtaacctgaa
     7801 aaacaccaaa tcccatcatc ggcattttca gattgttact taatacggaa aattccactg
     7861 tcagtccctc cattaggcat gaacaatgag tctacgttaa aacataacct caaattagta
     7921 tgtggatttt gatatcactt atgcaaaaaa ttcattaata atgtaggact gaaacctctc
     7981 tattttcggg gacaacgaag cagacgctac cagtgctttt gccttcgacc ttgctgtttt
     8041 tgatacactt agggccttgg gcataacgaa aatgtgcgat atcacgaaga attaatggag
     8101 aatg
//
LOCUS       DAZSBF010000186         8087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZSBF010000186 DAZSBF010000000
VERSION     DAZSBF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.189"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            85..1581
                     /gene="ravA"
                     /locus_tag="ACHXWD_003303"
     CDS             85..1581
                     /gene="ravA"
                     /locus_tag="ACHXWD_003303"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR7622135.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1575..3026
                     /gene="viaA"
                     /locus_tag="ACHXWD_003304"
     CDS             1575..3026
                     /gene="viaA"
                     /locus_tag="ACHXWD_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR7622136.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHXWD_003305"
     CDS             complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="ACHXWD_003305"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR7622137.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4175..4633
                     /gene="asnC"
                     /locus_tag="ACHXWD_003306"
     CDS             4175..4633
                     /gene="asnC"
                     /locus_tag="ACHXWD_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR7622138.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4723..5166
                     /gene="mioC"
                     /locus_tag="ACHXWD_003307"
     CDS             4723..5166
                     /gene="mioC"
                     /locus_tag="ACHXWD_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR7622139.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHXWD_003308"
     CDS             5545..7434
                     /gene="mnmG"
                     /locus_tag="ACHXWD_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR7622140.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7498..>8087
                     /gene="rsmG"
                     /locus_tag="ACHXWD_003309"
     CDS             7498..>8087
                     /gene="rsmG"
                     /locus_tag="ACHXWD_003309"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR7622141.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGER"
BASE COUNT         2018 a         2076 c         2077 g         1916 t
ORIGIN      
        1 ctttgagact attttagcca ccagcaagaa acgtctatac tcgcaattta cgcagaactt
       61 ttgacgaaag gacgccactt cattatggct caccctcatt tattagcgga aagaatttcc
      121 cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat ccgcttgtgt
      181 ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa
      241 agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg
      301 atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca ggcgctaaaa
      361 gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga aattgtcttt
      421 ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac cgccattaac
      481 gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct gctggtggcg
      541 gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga ccgcatgctg
      601 attcgtctgt ggttagataa agtgcaggat aaagcgaatt tccgctccat gctgaccagt
      661 caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga tgaagaatat
      721 gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt tgagctgatt
      781 tttatgctgc gcctgcaact ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc
      841 tggaaaaaag cgatccgatt attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt
      901 gccccggtag atctcatttt gctgaaagat tgcctgtggt atgacgcgca aagcctgaat
      961 ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca gcaagggatg
     1021 ttgactcgcc ttggcgcgat tgtgcaacgt cacctgcaac tccagcagca acaaagcgat
     1081 aaaacagcct taacggtaat tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa
     1141 cttcctgtta acgttactgc ttccactctg actctactgc tgcaaaaacc gttaaaactg
     1201 catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca gtggctgagc
     1261 aaaggtggtg aaattcgcgg taaactgaac ggtatcggct ttgcccagaa actgaatctg
     1321 gaagttgata gcgcccaaca tcttgtcgtt cgcgatgtaa gtttacaagg cagtacgctg
     1381 gcacttcccg gttcatcggc tgaaggtctg cctggtgaaa taaaacaaca actggaagag
     1441 cttgaaagcg actggcgcaa gcaacacgct ttattcagcg aacagcaaaa atgtctgttt
     1501 atccctggcg actggttagg tcgcattgaa gccagcctac aggatgtcgg tgcacagatt
     1561 caccaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc gtcagcgaag
     1621 agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg gcggtcttct
     1681 ttgaaaaatt cccacgcctg aaggcggcaa tcactgatga tgttccccgc tggcgtgagg
     1741 cgctgcgcag tcggctgaaa gatgcccgag tcccgccgga actcaccgaa gaggtgatgt
     1801 gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta ccacagatcc
     1861 tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag ttggttgatg
     1921 ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg cgtttaagtc
     1981 tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc gaacaactgt
     2041 tgagtgaagt tcaagaacgc atgacgctga gcggacaact tgaaccgatt ctcgcagata
     2101 acaatactgc agctggtcgt ctgtgggata tgagcgccgg tcagcttaaa cgtggcgact
     2161 atcagttgat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg
     2221 cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg
     2281 aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc
     2341 aacaaagcga tgatatttta cgtcttctgc cgccagaact ggcgacacta gggataacgg
     2401 aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc
     2461 acggtgagtc gtggcgtgaa aaagtgatcg aacgtccggt ggtacataaa gattacgatg
     2521 aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg ggcggcttta
     2581 atgaacagtg tgcgaaagcg ttctgcctgg ccttgatgcg cattgctctc gctgaaaacc
     2641 ggcgctgcta tattatgcta ttttccaccg agatcgtccg ttatgagctt tcaggccccc
     2701 aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc accgatcttg
     2761 ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt gatgccgatg
     2821 cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg agtaaagtga
     2881 aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca
     2941 aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg atgcgaagcc
     3001 gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgt tctcgcgaac
     3061 agcagctggc catacaccac actgaacctg gccgatatgc ggcagttgca gcagcagcat
     3121 ggtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac cgcgcagcag
     3181 cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca actgatgctt
     3241 caacgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat cttccagtac
     3301 agggttccac accagaatat cgccgttcag acccgcatga cccagctctg acggggtgct
     3361 ccagtcatca taatccggtg cgcgcacgtc gtggcgatga ccatcgctca gcttgccgcc
     3421 aatcccgaca aggaataccg cgccaagatc tttcgctatc gcccgctcac gccctttggc
     3481 atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgga tctgatccgg
     3541 caggaacggt gccaggccaa actcttcgct aaccgcagct tcggttgctt taattcccgc
     3601 ccagatcgcc tctaccgtgc ttttcagagt cgagaattga cgctcaccgt cgcccattac
     3661 gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac ggtcttcatc
     3721 ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc
     3781 taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg catcaggcag
     3841 agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct gcgtgccatc
     3901 ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt cttccagttg
     3961 gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt
     4021 cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc gacaaacatg
     4081 caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag aaggctaaaa
     4141 tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga tcgacaatct
     4201 ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg ccgaactggc
     4261 gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa tgaagcaggc
     4321 ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt atgacgtagg
     4381 ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc tggcaaagct
     4441 ggaaagcctt gctgaagtca ctgaagccta ttacacaaca ggccactaca gcatctttat
     4501 aaaagtaatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca agatccaaac
     4561 aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga tcatgcgtac
     4621 catcaagcca taatcggctt ttttaatccc atacttttcc acaggtagat cccaacgcgt
     4681 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
     4741 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
     4801 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
     4861 tcggggatct ggctgattat cagctccacc cacggtgccg gagatattcc ggacaacctt
     4921 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
     4981 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctatcga taaactcgag
     5041 gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat caacattctt
     5101 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
     5161 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
     5221 ccggtagtta tccaaggaac aacggttgtt cagtttttga gttgtgtata acccctcatt
     5281 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
     5341 gttgttgatc ttaaaagccg gatccttgtt atccacaggg tagtgcgatc ctaataagag
     5401 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
     5461 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
     5521 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
     5581 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
     5641 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
     5701 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
     5761 gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg accggcggtt
     5821 cgcgctaccc gagctcaggc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
     5881 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
     5941 gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg
     6001 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
     6061 ggtcgtgctg gtgatccgcc gtccattccg ctttctcgcc gtttgcgtga actgccgctg
     6121 cgcgttggtc gtctgaaaac cgggacacca ccgcgtattg atgctcgaac tatcgacttt
     6181 agtgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggcaat
     6241 gcgtcccagc atccccagca ggtgccgtgt tatatcactc ataccaacga gaaaacccat
     6301 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtacg caggggtgat cgaaggtgtc
     6361 ggcccacgct actgcccatc gatcgaagac aaagtcatgc gcttcgccga cagaaatcag
     6421 catcagatct tccttgaacc ggaagggctg acctctaacg aaatttatcc gaacggtatc
     6481 tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctccatgca ggggatggaa
     6541 aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga tccacgcgac
     6601 ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg tcagattaac
     6661 ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggtcggtct taacgctgcc
     6721 cgtctgtctg ctgacaaaga aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta
     6781 ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc
     6841 gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt
     6901 cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat
     6961 atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca
     7021 gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg tgaagatctg
     7081 ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg
     7141 ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga aggttatatc
     7201 gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg
     7261 acactggatt accgccaggt atccggtctt tctaacgaag tgatcgccaa acttaacgat
     7321 cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc tgcggctatc
     7381 tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt
     7441 aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg
     7501 ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttaccga tcaccagaaa
     7561 aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta caacctgact
     7621 tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat tgtggtggca
     7681 ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact gccaggcatt
     7741 ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct tggtaaacgc
     7801 gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga gccagtacag
     7861 agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag ccgcgctttt
     7921 gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca aggccgtttc
     7981 tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga agaatatcag
     8041 gtcgaatcag tggttaaact tcaggttcca gctctggatg gcgaacg
//
LOCUS       DAZSBF010000187         8023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZSBF010000187 DAZSBF010000000
VERSION     DAZSBF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.190"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHXWD_003310"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHXWD_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHXWD_003311"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHXWD_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR7622142.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHXWD_003312"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHXWD_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR7622143.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHXWD_003313"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHXWD_003313"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR7622144.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHXWD_003314"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHXWD_003314"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR7622145.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHXWD_003315"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHXWD_003315"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR7622146.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHXWD_003316"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHXWD_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR7622147.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1864 a         2063 c         2084 g         2012 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tag
//
LOCUS       DAZSBF010000188         8021 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZSBF010000188 DAZSBF010000000
VERSION     DAZSBF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.191"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            133..222
                     /gene="kdpF"
                     /locus_tag="ACHXWD_003317"
     CDS             133..222
                     /gene="kdpF"
                     /locus_tag="ACHXWD_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR7622148.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            222..1895
                     /gene="kdpA"
                     /locus_tag="ACHXWD_003318"
     CDS             222..1895
                     /gene="kdpA"
                     /locus_tag="ACHXWD_003318"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR7622149.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1918..3966
                     /gene="kdpB"
                     /locus_tag="ACHXWD_003319"
     CDS             1918..3966
                     /gene="kdpB"
                     /locus_tag="ACHXWD_003319"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR7622150.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3975..4547
                     /gene="kdpC"
                     /locus_tag="ACHXWD_003320"
     CDS             3975..4547
                     /gene="kdpC"
                     /locus_tag="ACHXWD_003320"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR7622151.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4540..7224
                     /gene="kdpD"
                     /locus_tag="ACHXWD_003321"
     CDS             4540..7224
                     /gene="kdpD"
                     /locus_tag="ACHXWD_003321"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR7622152.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7221..7898
                     /gene="kdpE"
                     /locus_tag="ACHXWD_003322"
     CDS             7221..7898
                     /gene="kdpE"
                     /locus_tag="ACHXWD_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR7622153.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1651 a         2099 c         2363 g         1908 t
ORIGIN      
        1 ctaaacatta gttaaatcat ggcttttgcc atttttatac tttttttaca ccccgcccgc
       61 agatttttgc gaaatctttg cagccagaat tctactcttc cggtatcact tttaggccac
      121 tggaggtgca ctgtgagtgc aggcgtgata gccggcgtgt tgctggtgtt tttattactg
      181 ggttatttgg tttatgccct gatcaatgcg gaggcgttct gatggctgcg caagggttct
      241 tactgattgc cacgttttta ctggtattaa tggtgctggc gcgtccttta ggcagcgggc
      301 tggcgcggct gattaatgac attcctcttc caggtacagc gggcgttgaa cgtatactct
      361 ttcgtttacc tggcgtctct gaccatgaga tgaactggaa gcaatatctt tgtgccattc
      421 tcggcctgaa catgctgggg ctggcggtgc tgttttttat gttgctcggt cagcactatc
      481 tgccgcttaa tccacagcag ttgccggggc tgtcgtggga tctggcgctg aataccgccg
      541 tcagctttgt cacgaatacc aactggcaat cttatagcgg tgaaaccacg ttgagctatt
      601 tcagccagat ggcaggctta acggtacaaa actttctttc tgccgccagc gggattgcgg
      661 tgatttttgc cttcatccgt gcgtttactc gccagagcat gagcacactc gggaatgcct
      721 gggtcgatct gctacgcatc acgttatggg tgctagtccc tgtggcgttg ttgatcgcac
      781 tgttttttat tcaacaaggt gcgctgcaaa actttctgcc ctatcaggca gtgaataccg
      841 ttgaaggagc gcaacagctg ttacccatgg gaccagtagc ttctcaggaa gcgatcaaga
      901 tgctcggtac taacggcggt ggcttcttta atgccaactc gtcgcatccg tttgaaaacc
      961 caaccgcact gaccaacttc gtgcagatgc tggcgatctt cttgatccca acagcgctgt
     1021 gctttgcctt tggtgaagtg acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga
     1081 tgtcagtgat ttttgtcatc tgcgtaggcg tggttatgtg ggcagaagtt cagggtaatc
     1141 ctcatctgct ggcgctcggc gcagacagca gcatcaatat ggaaggtaaa gagagccgtt
     1201 tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac ggcggcgtcc tgtggcgcgg
     1261 tgattgcgat gcatgattcg tttaccgctc tcggcggcat ggtgccgatg tggctaatgc
     1321 aaattggtga agtggtgttc ggcggtgtcg gttctggtct ttacggcatg atgctgtttg
     1381 tcctgctggc ggtgtttatt gccgggctga tgattggtcg tacaccggaa tatctgggta
     1441 aaaaaatcga cgtacgcgag atgaaactga ctgcactggc gattctggtt actccaacac
     1501 tggtactgat gggcgcggcg ttggcgatga tgaccgacgc cggacgtagc gccatgctta
     1561 accctggccc gcatggtttt agcgaagtgc tgtacgccgt gtcgtccgcc gctaacaaca
     1621 acggcagcgc ctttgccgga ttaagcgcca actctccgtt ctggaactgt ttactggcgt
     1681 tctgcatgtt tgtcggtcgc ttcggggtga ttatcccggt gatggcaatt gccggttcgc
     1741 tggtgagtaa aaagagccaa cccgccagct ccggcacgct gccaacgcac ggcccgctgt
     1801 ttgttggcct gttaatcggc accgtgttgc tggttggcgc actgaccttt atccctgccc
     1861 tggcgcttgg tccggtggcg gaatatctct cctgatgata ttgagcgagc actgaatatg
     1921 agtcgtaaac aactggcgct attcgaacca acacttgtcg ttcaggcgct gaaagaagcg
     1981 gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc
     2041 ggcagtctgc tgaccacctg tattagcatc gcgatggcaa gcggtgcgat gcctggcaat
     2101 gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc
     2161 gccgaggcgc tggcagaagg ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa
     2221 aaaactgcct ttgcccgcaa gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt
     2281 cctgccgatc aactgcgtaa aggtgacatc gtgctggtag aagctggcga tattatcccc
     2341 tgcgatggtg aagtcattga aggcggcgca tcggtcgatg aaagcgccat caccggggaa
     2401 tcggcaccgg tgatccgtga atccggcggc gattttgcct ccgtcaccgg cggcacgcgt
     2461 attctttctg actggctggt gattgagtgt agcgttaacc ccggtgagac atttctggat
     2521 cggatgatcg cgatggtgga aggcgcacag cgacgcaaaa caccgaacga gattgccctg
     2581 actatcctgc tgattgctct gaccatcgtc tttttactgg caaccgccac gctgtggccg
     2641 ttttccgcgt ggggcggtaa tgccgtgagt gtaacggtac tggtggcgct gctggtctgt
     2701 ctgatcccaa ccactattgg cggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg
     2761 atgctgggcg cgaatgtgat tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt
     2821 gacgttctgc tactggataa aaccggcacc atcacactcg gtaaccgtca ggcgtcggag
     2881 tttatccccg cgcagggcgt ggatgaaaaa acgctggctg acgccgcaca actggcttcg
     2941 ctggctgatg aaacgccgga aggtcgcagt attgtgatcc tcgccaagca acgttttaac
     3001 ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg taccgtttac tgcgcaaagc
     3061 cggatgagcg ggatcaacat cgacaaccgc atgatccgta aaggttctgt cgatgccatt
     3121 cgccgccata ttgaggctaa cggcggtcac ttccctgccg atgttgatca aaaagtcgat
     3181 caggttgcgc gtcagggagc cacgccgctg gtggtggtgg aaggttctcg tgtgctgggc
     3241 gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc
     3301 aaaatgggca ttaaaacggt gatgattacc ggcgataacc gtctgactgc cgccgcgata
     3361 gctgcggaag cgggtgtcga tgattttctc gccgaagcaa caccggaggc caagctggca
     3421 ttgatccgtc agtatcaggc ggaaggtcgt ttggtagcga tgaccggcga cggcaccaac
     3481 gatgctccgg cgctggcgca ggcagatgtc gcggtggcga tgaactccgg cacccaggcg
     3541 gcgaaagagg cgggcaatat ggtggatctc gactctaacc cgaccaagtt gatcgaggtg
     3601 gtgcacattg gcaaacagat gctgatgacc cgtggctcgc tgaccacctt cagcattgcc
     3661 aacgatgtgg cgaaatactt cgccattatt ccggcggcgt tcgcggcaac atatccgcag
     3721 ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact ccgccattct cagtgcggtc
     3781 attttcaacg ccttgattat cgtctttttg attcctctgg cgttaaaagg cgtgagttat
     3841 aaaccgctta ccgcttctgc catgttgcgc cgtaacttat ggatttacgg tctgggtggg
     3901 ctgctggtgc cgtttattgg tatcaaagtc attgatttac tgctgaccgt ttgcggtctg
     3961 gtgtgaggtt taccatgagt ggattacgtc cggcattatc gacatttctc tttctgttat
     4021 tgattactgg cggcgtttac ccgctgctga ccaccgcact ggggcaatgg tggtttccct
     4081 ggcaggccaa cggttcgttg atccgtgaag gtgatacggt gcgtggctcg gcattaatcg
     4141 ggcagaattt taccggcaac ggctattttc atggtcgccc gtcagcaacg gctgaaatgc
     4201 cctataatcc acaggcttct ggcgggagca atctggcggt cagtaaccct gagctggata
     4261 aactaatagc cgcacgcgtt gctgcattac gggccgctaa cccggatgcc agcgcgagca
     4321 ttccggttga actggtgacg gcatcggcaa gcgggctgga caataatatc accccgcaag
     4381 cggcggcctg gcagatccca cgcatcgcga aagcgcgtaa tctcagcgtt gaacagctca
     4441 cgcagctgat cgcaaaatac agccaacaac cgctggtgaa atatatcggc cagccggtcg
     4501 tcaacattgt cgaactcaat ctggcgctgg ataagcttga tgaataacga acccttacgt
     4561 cccgaccccg atcgtctgct ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt
     4621 ttcttcggtg cctgtgcagg cgtcgggaag acctgggcga tgctggcaga agcccagcga
     4681 ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag aaacccacgg gcgaaaagat
     4741 accgccgcaa tgctggaagg gctggctgtt ctgccgccaa aacgcctggc gtaccgtggg
     4801 cggcacatct gcgagtttga cctcgatgcc gccctcgccc gccgtccggc gctgatctta
     4861 atggacgaac tggcgcacag taatgcgcca ggttctcgtc accctaaacg ctggcaggac
     4921 atcgaagagc tgctggaagc aggcattgat gttttcacta ccgtcaacgt tcagcatctg
     4981 gaaagtctga atgatgtggt cagcggcgtc accggaattc aggtacggga aaccgtgccc
     5041 gatccttttt tcgatgccgc cgacgacgtg gttctggtgg acttgccccc ggacgatctg
     5101 cgccagcggc tgaaagaagg caaagtctat attgccgggc aggcggagcg cgccattgaa
     5161 cattttttcc gcaaaggcaa tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc
     5221 gatcgcgttg atgagcaaat gcgcgcctgg cgggggcatc ctagcgaaga gaaagtctgg
     5281 cacacgcgcg acgcgatcct tttatgcatc ggccataaca ccggtagtga gaagctggtc
     5341 cgcgcggcgg cgcggctggc gtcacggctg ggtagcgtct ggcacgcggt gtatgttgaa
     5401 acccctgccc tgcatcgctt accggagaaa aaacgtcgtg caattctcag cgccttacgt
     5461 ctggcgcagg aactgggcgc ggagacggca acactttctg atccagcgga agagaaagcg
     5521 gtagtgcgtt atgcccgtga acataatctc ggcaagatta ttctcggtcg cccggcctcg
     5581 cgccgctggt ggcgtcggga aacgtttgct gaccgactgg cgcgcatcgc ccccgatctc
     5641 gatcaggtgc tggtcgcgct tgatgaacca cccgcccgca cgattaacaa cgcgccggat
     5701 agccgctctt ttaaagacaa gtggcgggta cagattcagg gatgcgtggt tgccgccgcg
     5761 ttatgcgccg ttatcacctt aattgccatg cagtggctga tggcgtttga tgccgccaac
     5821 ctggtgatgc tgtatctgct tggcgtggtg gtggtggcgc tattttatgg acgctggcct
     5881 tcggtggttg ccaccgtcat taatgtggtg agtttcgatc tcttttttat cgccccacgc
     5941 ggcacgctcg ccgtctctga tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc
     6001 gggctggtga tcgggaacct tactgctggc gtgcgttatc aggcgcgggt agcccgttac
     6061 cgcgagcaac gcacacggca cttatatgaa atgtcgaaag ctctggcggt gggccgcagt
     6121 ccgcaggaca tcgccgccac cagcgaacaa tttattgcct ccacgtttca tgcccgcagt
     6181 caggtgttgt tgcccgatga caacggtaaa ttgcagccgt taacacatcc gcaaggaatg
     6241 acgccatggg acgatgccat cgcgcagtgg agttttgata aaggcctgcc tgcgggcgcg
     6301 ggcaccgaca cgttacccgg tgtaccgtac cagattttgc cgctaaaaag cggcgagaaa
     6361 acctacgggc tggtggtggt ggagccgggg aatctgcgcc agttgatgat cccggaacag
     6421 cagcgcctgc tggagacgtt tacgctgtta gtcgccaatg cccttgagcg gctgacgcta
     6481 accgccagcg aagaacaggc gcggatggca agcgaacgtg aacagatccg caacgccctg
     6541 ctggcagcgc tttcgcatga tttacgcacg ccgcttacgg tgctgtttgg tcaggcagaa
     6601 atcttaacgc tcgatctggc aagcgaagga tcaccccacg cccgccaggc cagcgagatt
     6661 cgtcagcatg tgctgaacac tacccgactg gtgaataatc tactggatat ggcgcgaatt
     6721 cagtccggcg gctttaattt gaagaaagag tggttaacgc tggaagaagt agtcggcagc
     6781 gcgctgcaaa tgctggaacc gggtttatcg tcgcccatca atctttctct gccagaaccg
     6841 ctgaccttaa tccacgttga cgggccactc tttgaacggg tgctgattaa tctgctggag
     6901 aacgcggtga aatatgcggg tgcgcaggcc gaaattggta tcgatgccca ctttgagggc
     6961 gaaaatctac aactggatgt ctgggataac ggccccggtc ttccgccagg ccaggagcag
     7021 acgatatttg ataagtttgc tcgcgggaat aaagagtcgg cggtgccggg tgtcgggctt
     7081 gggttggcaa tttgtcgggc gattgtggat gtacacggcg gcaccattac cgcgttcaac
     7141 cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc agcaaaccgc tcctgaactt
     7201 gaagactttc atgaggatat gtgacaaacg ttctgattgt tgaagatgaa caggctattc
     7261 gtcgctttct gcgcacggcg ctggagggcg acgggatgcg cgtctttgag gccgaaactc
     7321 tgcaacgcgg cttgctggaa gcggcaaccc gtaagccaga tttgattatt ctcgatctcg
     7381 gcctgcccga tggtgatggg attgagttta tccgcgacct gcgccagtgg agcccggtgc
     7441 cggtgattgt gctttccgca cgcagcgaag agagcgacaa gatcgccgcg ctggatgccg
     7501 gagcggatga ttatctgagt aagccgtttg gcattggcga attgcaggcc cgtctgcgcg
     7561 tcgctttacg ccgccactcc gccaccgccg cgcccgatcc gctggtgaaa ttttccgatg
     7621 ttaccgtcga tttagccgcc cgcgtgattc accggggtga ggaagaggtg catctcacac
     7681 caattgagtt ccgcctgctg gcggtgctgc tcaacaatgc cggaaaagta ctcacccagc
     7741 gccagctcct taaccaggtg tgggggccaa acgcggtcga acacagtcac tatttgcgta
     7801 tttatatggg acatctgcga caaaaactgg aacaggatcc ggcccggcct cgccacttca
     7861 ttactgaaac cggtattgga tatcggttta tgctttgaat tgttattcaa taaattcgct
     7921 taaatagtga ttaatttatt attaaacctg cgccgaatat tattatttgg caggtttaat
     7981 ttcttaacgc aaatataaaa cacgaaatta caacatttaa g
//
LOCUS       DAZSBF010000189         7968 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZSBF010000189 DAZSBF010000000
VERSION     DAZSBF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.192"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHXWD_003323"
     CDS             <1..239
                     /locus_tag="ACHXWD_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR7622154.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="ACHXWD_003324"
     CDS             279..1142
                     /locus_tag="ACHXWD_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR7622155.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHXWD_003325"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="ACHXWD_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR7622156.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="ACHXWD_003326"
     CDS             2679..4037
                     /locus_tag="ACHXWD_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR7622157.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="ACHXWD_003327"
     CDS             4180..5130
                     /locus_tag="ACHXWD_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR7622158.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="ACHXWD_003328"
     CDS             5140..6522
                     /locus_tag="ACHXWD_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR7622159.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6738..6812
                     /locus_tag="ACHXWD_003329"
                     /pseudo
     CDS             6738..6812
                     /locus_tag="ACHXWD_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="ACHXWD_003330"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="ACHXWD_003330"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR7622160.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2019 c         2308 g         1722 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggcgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAZSBF010000190         7959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZSBF010000190 DAZSBF010000000
VERSION     DAZSBF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.193"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(156..419)
                     /gene="cedA"
                     /locus_tag="ACHXWD_003331"
     CDS             complement(156..419)
                     /gene="cedA"
                     /locus_tag="ACHXWD_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR7622161.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            602..2863
                     /gene="katE"
                     /locus_tag="ACHXWD_003332"
     CDS             602..2863
                     /gene="katE"
                     /locus_tag="ACHXWD_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR7622162.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3121..3879)
                     /gene="chbG"
                     /locus_tag="ACHXWD_003333"
     CDS             complement(3121..3879)
                     /gene="chbG"
                     /locus_tag="ACHXWD_003333"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR7622163.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3892..5244)
                     /gene="celF"
                     /locus_tag="ACHXWD_003334"
                     /gene_synonym="chbF"
     CDS             complement(3892..5244)
                     /gene="celF"
                     /locus_tag="ACHXWD_003334"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR7622164.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5349..6191)
                     /gene="chbR"
                     /locus_tag="ACHXWD_003335"
     CDS             complement(5349..6191)
                     /gene="chbR"
                     /locus_tag="ACHXWD_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR7622165.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6199..6549)
                     /gene="chbA"
                     /locus_tag="ACHXWD_003336"
     CDS             complement(6199..6549)
                     /gene="chbA"
                     /locus_tag="ACHXWD_003336"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR7622166.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6600..7958)
                     /gene="chbC"
                     /locus_tag="ACHXWD_003337"
     CDS             complement(6600..7958)
                     /gene="chbC"
                     /locus_tag="ACHXWD_003337"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR7622167.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
BASE COUNT         2143 a         2079 c         1780 g         1957 t
ORIGIN      
        1 aaaataaata aacaattaaa gaaaaaagat cacttattta tagcaatagc tcgtcaaagg
       61 cagctttttg ttacaggtgg tttgaatgaa tgtagcaacg aaatgcagaa tttcaggtca
      121 tgtaactccc ggcaaaaccg ggaagtatgt aatccttact cagtcacttc cccttcctgg
      181 cggatctgat ttgcccaacg ttgggcagat tcaggcacag taaacgccgg tgagcgcaga
      241 aatgactctc ccatcagtac aaacgcaaca tatttgccac gcagcatcca gacatcacga
      301 aacgaatcca tctttatcgc atgttctggc ggcgcgggtt ccgtgcgtgg gacatagcta
      361 ataatctggc ggttttgctg gcggagcggt ttcttcatta ctggcttcac taaacgcata
      421 ttaaaaatta gaaaaactgt agtttagccg atttagcccc tgtacgtccc gctttgcgtg
      481 tatttcataa caccgtttcc agaatagtct ccgaagcggg atctggctgg tggtctatag
      541 ttagagagtt ttttgaccaa aacagcggcc ctttcagtaa taaattaagg agacgagttc
      601 aatgtcgcaa cataacgaaa agaacctaca tcagcaccag tcaccactac acgattccag
      661 cgaagcgaaa ccggggatgg actcactggc acctgaggac ggatctcatc gtccagcggc
      721 tgaaccaaca ccgccaggtg cacaacctac cgcccctggg agcctgaaag cccctgatac
      781 acgtaacgaa aaacttaatt ctctggaaga cgtacgcaaa ggcagtgaaa attatgcgct
      841 gaccactaat cagggcgtgc gcatcgccga tgatcaaaac tcactgcgtg ccggtaaccg
      901 tggtccaacg ctgctggaag attttattct gcgcgagaaa atcacccact ttgaccatga
      961 gcgcatcccg gaacgtattg ttcatgcacg cggatcagca gctcacggtt atttccagcc
     1021 atataaaagc ttaagcgata tcaccaaagc ggatttcctc tcagatccga acaaaatcac
     1081 cccagtattt gttaggttct ctaccgttca gggtggcgct ggctctgcag ataccgtgcg
     1141 tgatatccgt ggttttgcca ccaagttcta tactgaagag ggtatttttg acctcgttgg
     1201 caataacacg ccaatcttct ttatccagga tgcgcataaa ttccccgatt ttgttcatgc
     1261 ggtaaaacca gaaccgcact gggcaattcc acaagggcaa agtgcccacg atactttctg
     1321 ggattatgtt tctctgcaac ctgaaactct gcacaacgtg atgtgggcga tgtcggatcg
     1381 cggcatcccc cgcagttacc gcaccatgga aggcttcggt attcacacct tccgcctgat
     1441 taatgccgaa gggaaggcaa cgtttgtacg tttccactgg aaaccactgg caggtaaagc
     1501 ctcactcgtt tgggatgaag cacaaaaact caccggacgt gacccggact tccaccgccg
     1561 cgagttgtgg gaagccattg aagcaggcga ttttccggaa tacgaactgg gcttccagtt
     1621 gattcctgaa gaagatgaat tcaagttcga cttcgatctt ctcgatccaa ccaaactgat
     1681 cccggaagaa ctggtgcccg ttcagcgtgt cggcaaaatg gtgctcaatc gcaatccgga
     1741 taacttcttt gccgaaaacg aacaagcagc attccatcca ggtcatattg ttcctggtct
     1801 ggacttcacc aacgatccgc tgttgcaggg acgtttgttc tcctataccg atacacaaat
     1861 cagtcgtctt ggtgggccga atttccatga gattccgatt aaccgtccga cctgccctta
     1921 ccacaacttc cagcgtgatg gcatgcatcg tatggggatc gacactaacc cggcgaatta
     1981 cgaaccgaac tcgatcaacg ataactggcc gcgcgaaaca ccgccgggac cgaaacgcgg
     2041 cggttttgaa tcatatcagg aacgcgtgga aggaaataaa gttcgtgagc gcagtccatc
     2101 gtttggcgaa tattattccc atccgcgtct gttctggcta agtcagacgc cattcgagca
     2161 gcgccatatt gtcgatggtt tcagttttga gttaagcaaa gtcgttcgtc cgtatattcg
     2221 tgagcgcgtt gttgaccagc tggcgcatat tgatctcact ctggcccagg cggtggcgaa
     2281 aaatctcggt atcgaactga ctgacgacca gctgaatatc accccacctc cggacgtcaa
     2341 cggtctgaaa aaggatccat ccttaagttt gtacgccatt cctgacggtg atgtgaaagg
     2401 tcgcgtggta gcgattttac ttaatgatga agtgagatcg gcagaccttc tggccattct
     2461 caaggccctg aaggccaaag gcgttcatgc caaactgctc tactcccgaa tgggtgaagt
     2521 gactgcggat gacggtacgg tgttgcctat agccgctacc tttgccggtg caccttcgct
     2581 gacggtcgat gcggtcattg tcccttgcgg caatatcgcg gatatcgctg acaacggcga
     2641 tgccaactac tacctgatgg aagcctacaa acaccttaaa ccgattgcgc tggcgggaga
     2701 cgcgcgcaag tttaaagcaa caatcaaggt cgctgaccag ggtgaagaag ggattgtgga
     2761 agctgacagc gctgacggta gttttatgga tgaactgcta acgctgatgg cagcacaccg
     2821 cgtgtggtca cgcattccta agattgacaa aattcctgcc tgatgggagg cggcgcaatt
     2881 gcgccgcctc aaatgattta catagtgcgc tttgtttatg ccggatgcgg cgtgaacgcc
     2941 ttatccggcc tacaaaactg tgcaaattca atatattgca ggaaacacgt aggcctgata
     3001 agcgaagcgc atcaggcagt tttgcgtttg tcagcagtct caagcggcgc agttacgccg
     3061 cctttgtagg aattaatcgc cggatgcaag gttcacgccg catctggcaa acatcctcac
     3121 ttacacatcc cgataactcc ccaaccgata accacgctga gcgatagcac ctttcaacga
     3181 cgctgatgtc aacacatcca gctccgttaa gcgtgggaaa cagtaagcac tctgacggat
     3241 agtattatcg ataaacgccg gatgacacat cacctccagc gaacgatcac cccgatggct
     3301 tgcgtcatcc agcacttgca ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct
     3361 actgaatccc tgagaactgc gcagattagc cggtaaatca ccagagttag atagcggctg
     3421 acggtcgatg cgcaacgcaa tcccctcttc agccgcaaac ctggcaacaa tcgggaaaat
     3481 ctgcgggaac atatgcacat gatgatggct atcaagatgc gtaggtttgc gtccaaatag
     3541 ctcaatgaaa cgcaaatact gactggcaag ctcctgagta atttcttcca gcggtaaagc
     3601 atcttcttct gccaactgcc agatccattt tcccagcaca ccatcgcggg ttaaccccgg
     3661 catagctgtc agtggcttgc ccatagtaag gacaaagtac atccctatgg ccagactcgg
     3721 ttcatcacga ctcaactgca ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt
     3781 cgatgtgaca atcccattgc gacaggcctc gataatgccg tagttctgac ctttgcttaa
     3841 gccaaaatca tcggcattaa caatcagtaa gcgttccata atcagcctcg gttaatgtgc
     3901 ttttttaagc tctgcgatgc agtcggcaaa atttggcagc catttctcgt gcgccagaat
     3961 catctcgcgt gccagcagct cagcatcgcg atcggaatgc accaacggac taaggtttag
     4021 cgccagtaac acatcgttaa attctccgct aagtgcggcg ttgctggcag caatctcgaa
     4081 gcctttaatg gtgtgaatca gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg
     4141 tggcgtcgcg ccatcgcgcc ccagcttaca ggtcatttct accgcccagt ctgccggaat
     4201 attatcaata tgcccatgat gcgggatatt aacgtaatgt tcagcttgct tgtcgttgta
     4261 gatagcgttg atcacttcgc acgctgcatc agagtaataa gccccaccgc gctgttccag
     4321 ttctttcggc ttaactttca actccgggtt tttatacagc tcaaaaagtt gtttctcgac
     4381 tttctgtact acctgcgctc gtgcgccgcc tttgtagtat tcgcccattt caatagccag
     4441 catctctttt tgcttgaagt aataaagcaa ataagaacac ggcaacagat tcagagaacg
     4501 aattaagcct tcgctaaatg gcagatcgaa aatattttta acgccagatg cttttaactg
     4561 cccggatgcc acaccatcaa gcaattcggc aaagcgcgac ttgccattta ccagcacatc
     4621 cttaatgaac accatatggt tgaggccgaa caaatcgata gataaatcat cgctgtcttt
     4681 cagcatcaga acatcgtgaa taaacatctt catgccgatc ggaatattac acacgccgat
     4741 aaagcgttta aatccggtat gacgataaac ggcttcagta accattcccg ccgggttagt
     4801 gaagttaatc acccatgcat tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat
     4861 caccggaatg gtacgcagac ctttaaacag accgcccgcg ccgttggttt cctggccaag
     4921 ataaccatga cttaatggaa tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg
     4981 cagttgggta gtaacgaaat cagcatcttt caatgcttcg cggcgatcca gcgttttata
     5041 aagcttcatc gggacgccag cgttatcaat catccgttgg cagagttcaa aaatgatatc
     5101 cagtttctct ttaccatctt cgacatccac cagccataat tcgctgaccg gcaattcgtg
     5161 ataacgctta ataaatcctt ccagtaactc cggggtatag ctgctcccgc caccaatagt
     5221 gacgactttt aatttctggc tcataatttc tcccttcagt acagaatact gatatctggc
     5281 atatctgccc ccccggacat aaataatcca gcaacaggac agatatgtga attgtcaggt
     5341 ataacgactt actgattaaa ttcagtcaat ttcttacgat agctcttagg cgtaaaggac
     5401 gttaatttct taaacgtttt aataaacaaa ctcgggctac tataaccggc ctcaaacgca
     5461 atatccgtca cggaatagtt ggtcatttcc agttgttttt tggcaaaatt aatacggatc
     5521 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
     5581 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
     5641 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
     5701 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
     5761 tccagccctg tttcgcggaa attcaatgaa gaaatcactg tttccacata ggtgagaaaa
     5821 gcgttattgg tccggtatac ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa
     5881 tgctgctcaa aaaagcgttt actgatccca acgttcaata tacgcgtggc accaaactca
     5941 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
     6001 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
     6061 tagtcgtgct gatgcagccc gctgatactc tcagttttgt tatagataaa cacatggaag
     6121 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
     6181 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
     6241 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
     6301 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
     6361 ttcattcaat gccatccgtg actgatccat catggctttt gctgcagcaa aatcgccctg
     6421 cttcgcctgt ttcagtgccg catacgccag gctgcgcgct tgtccggagt tgatgataag
     6481 ccccatcacc acttcttcca gctcttcagc ttccgtttgc gtatcgggaa tgttatcgag
     6541 atccatcata catcgttcct ctctattctt accggcacga ttacccgtac cggcatcgat
     6601 taaaatttca gggcgttagc gatatcttct tcgcgctctt ctttatcaat cgcgttttgt
     6661 gctttgttag ccaccacaac aaagggcaga taaattaacg ttgcgatacc aaggttgaag
     6721 agtgcaacca gcaatgcggc gacgctaccg ttggtgttaa agaaggctcc cagaccggtt
     6781 ggcatggtcc acggtgcaat attggtcacc ggaggaatga tgcccatgta gtacgctgcg
     6841 agggtgattg ccgccagaat cggttgtacc agtacaaacg ggataaacat caccgggttc
     6901 atgataattg gcagaccaaa cagaatcggt tcgttaatct ggaagatgcc ggacggcagc
     6961 gccagttttg ccacctgacg ataatcagca cggcgagagg cgataaagat agccaggatc
     7021 aggcctaaag tcgcaccact gcccccaagg aaaataaagg agtccagcat cggcttggcc
     7081 cagatatgga aggtcttacc ggctgccagc gccgcttcga cggaaccata ttgctgatag
     7141 gtcgcgatat tttccagcgc ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc
     7201 agcgcgccat gaataccgaa gaaccagagc agtggaacaa agatcacata ggcccagccc
     7261 accacgctac ccaacgatgc cagtggggtt gagatggtat ccataatgat ctgatggaag
     7321 ttggtgcccc aggtattcaa cgcccaggca ataatcccca tcacggaaag aataataaag
     7381 ccgggaatta atgccgagaa ggaacgcgat actgaagcag gtacgctatc gggcaattta
     7441 atgacccaat tgcggcggac aataaaggta aacatttctg ccaccaccag gccaataata
     7501 atcccggaga tgatattcgc tccacctaac cagtttgcgc caaccacata ggcctcaccg
     7561 acactatatg gggtgacggt cataaatgct gcaacggata acaacccagc cgccagcgca
     7621 tcgactttac gctcttctgc cagcgccatg ccaataaaga acggtgccat taaagacatt
     7681 atccctaatg ttccgttata tacgttgccg ccaatacctt tcaggccatt aagtgtttca
     7741 atggttgagg cgtcgaggcg aatacctaag gaataaaaaa acgccccctc cccaaagctt
     7801 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     7861 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     7921 aggagtacct tttcaagcga tgcaataaca ttactcata
//
LOCUS       DAZSBF010000191         7955 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZSBF010000191 DAZSBF010000000
VERSION     DAZSBF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.194"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            163..317
                     /locus_tag="ACHXWD_003338"
                     /pseudo
     CDS             163..317
                     /locus_tag="ACHXWD_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            363..779
                     /gene="hicB"
                     /locus_tag="ACHXWD_003339"
     CDS             363..779
                     /gene="hicB"
                     /locus_tag="ACHXWD_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR7622168.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            858..2264
                     /locus_tag="ACHXWD_003340"
     CDS             858..2264
                     /locus_tag="ACHXWD_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFR7622169.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2509..3654
                     /gene="ydcS"
                     /locus_tag="ACHXWD_003341"
     CDS             2509..3654
                     /gene="ydcS"
                     /locus_tag="ACHXWD_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR7622170.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3672..4685
                     /locus_tag="ACHXWD_003342"
     CDS             3672..4685
                     /locus_tag="ACHXWD_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR7622171.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4686..5586
                     /locus_tag="ACHXWD_003343"
                     /pseudo
     CDS             4686..5586
                     /locus_tag="ACHXWD_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5576..6370
                     /locus_tag="ACHXWD_003344"
     CDS             5576..6370
                     /locus_tag="ACHXWD_003344"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR7622172.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6392..7816
                     /gene="patD"
                     /locus_tag="ACHXWD_003345"
     CDS             6392..7816
                     /gene="patD"
                     /locus_tag="ACHXWD_003345"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR7622173.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         1848 a         1888 c         2196 g         2023 t
ORIGIN      
        1 ttcagttttg caaattagcg caaagaaatt ctggaatctt ccttcctgat tttgcattgc
       61 attctgccgt tgcggcgatt tagtgctatt ttcgagcaaa ttacacatag gggtaaacac
      121 taatgtttac tttattgata tactcagcgg cagggaggcg atgtgaaaca aagcgagttc
      181 agacgttggc tcgaatctca gggcgtcgat gtagcgaatg gccgcaacca tttgaaactc
      241 aggtttcatg ggaggcgcag tgtcatgccg cgtcactcct gcgtaaagca atcctgaaac
      301 aactcggttt gagttaatcg ccaattaaaa aggttaatga catgcgagag acagtcgaaa
      361 ttatgcgtta tcccgtcact cttacacccg cgccggaagg cggttatatg gtttcttttg
      421 tggatatccc tgaagcgttg acgcagggcg aaactgtcgc tgaagcgatg gaagcggcaa
      481 aagatgcttt actgaccgca tttgattttt attttgaaga taacgagctt atcccgttac
      541 cttcgccatt aaatagtcat gatcacttta ttgaagtacc tttgagcgtc gcctctaaag
      601 tattgctgtt aaatgctttt ttacagtcag aaatcactca gcaagagtta gccaggcgaa
      661 ttggcaaacc taaacaggag attactcgcc tatttaactt gcaccatgcg acaaaaatcg
      721 acgccgtcca gcttgcggca aaggcgcttg gcaaagagtt atcgctggtg atggtttaat
      781 tacagttaac gaaaagttgt catttttaac aactgatata gactgccgaa tcatctgcac
      841 ataattacga ttcgataatg aaaaaatacc agcagcttgc agaacaatta cgcgagcaga
      901 ttgcgtcggg tatctggcaa cccggcgatc gtttgccttc gttgcgtgac caggtggcgc
      961 tttcaggcat gagctttatg actgtcagcc atgcctatca gttgctcgaa agtcagggat
     1021 atattatcgc acgaccgcag tcgggttatt acgttgcgcc acaggcaata aaaatgccga
     1081 aagcgccagt cattccagtc actcgagatg aagcagtcga tatcaacact tatatttttg
     1141 atatgttgca ggccagtcgc gaaccgtcgg tcgttccgtt tgcctcggcc tttcccgacc
     1201 cgcgactttt ccccctccaa caactaaacc gctcgctggc gcaggtaagc aaaaccgcca
     1261 cagcgatgag cgtgattgaa aacttaccgc caggaaacgc agaactgcgt caggctattg
     1321 ctcgtcgcta tgccttacag ggcatcacca tttctcctga tgaaattgtc attactgccg
     1381 gggcgttaga ggcattaaac ctcagtttgc aagcggtaac tgaaccgggc gattgggtga
     1441 tagtagagaa tccttgtttc tacggtgcgt tgcaggcgct ggagcggcta cggctgaagg
     1501 cgttatcggt ggcgacggat gttaaagaag ggatcgatct tcaggcgctg gaactggcgt
     1561 tgcaggatta tccggtgaaa gcgtgctggc tgatgactaa tagccagaac ccactcggat
     1621 ttaccttaac gccgcaaaaa aaagcacaac tggtggcgtt actcaatcag tacaacgtaa
     1681 cgctgattga agatgacgtt tacagcgaac tttattttgg acgggaaaaa ccgctgcctg
     1741 cgaaagcgtg ggatcgccac gatggcgttt tgcattgctc ttcgttttcg aaatgtctgg
     1801 tgcctggttt tcgtattggt tgggtcgccg ccggaaaaca tgcacgtaaa attcaacgct
     1861 tgcagttgat gagtacgctt tccaccagct caccgatgca acttgcgctg gtggattacc
     1921 tttccacgcg ccgatacgac gcccatcttc gtcgcctgcg tcgccagctt gcggaacgta
     1981 aacaacgtgc ctggcaggca ctgctgcgtt atctgcctgc ggaagttaaa attcatcata
     2041 gtgacagcgg ttattttctc tggctggagc tccccgagcc gttagatgcc ggcgaattaa
     2101 gcctggcggc actgacgcat catatcagta ttgcgccggg taaaatgttt tctaccggtg
     2161 aaaactggtc acgttttttt cgttttaata ccgcgtggca gtggggagaa cgtgaagaac
     2221 aggcggtaaa acaattaggc aaacttattc aagaacggct gtaatagcgt ttaatttaat
     2281 tcctcttaga ttgggtaata tgaatttcga atagcagtca tatttcctaa ccccttgtct
     2341 atactccaga agataacctt acagacggca taatgcgcgg tagctcacaa cctgattaaa
     2401 ttttctcagg gacgaaggtg tgcctgcaag ccgccgtcta tggttaaaca aggagatatt
     2461 tttacggcac ggcggctgaa caattaatta cgacaggagt aagaccttat gagcaagaca
     2521 tttgcccgca gcagcctgtg tgcgctcacc atgacaataa tgaccgctca cgccgccgaa
     2581 ccacctacca atttagataa accggaaggg cgactggata ttatcgcctg gccgggatac
     2641 atcgaacgcg gacaaactga taaacaatac gactgggtaa cgcaatttga aaaagagaca
     2701 ggctgcgcgg tgaatgtgaa aaccgccgcg acttccgatg aaatggtcag tctgatgacc
     2761 aaagggggtt acgatctggt tacggcatcc ggcgatgcct cgctgcgttt gattatgggc
     2821 aaacgcgtgc agccgattaa taccgccttg attcccaact ggaaaacgct cgatccgcgc
     2881 gtggttaaag gcgactggtt taatgttggc ggcaaagttt acggcacacc ttaccaatgg
     2941 gggccgaacc tgctgatgta caacactaaa accttcccga cgccgccgga tagctggcaa
     3001 gtggtttttg ttgagcaaaa tctgccggac ggcaagagca ataaaggccg cgttcaggct
     3061 tatgatggcc ctatctacat tgcggacgct gcgttgttcg ttaaagccac tcagccgcag
     3121 ttgggcatca gcgatccgta tcaactaacc gaagaacagt accaggcggt gctgaaagtg
     3181 ctgcgcgctc aacacagttt gatccatcgc tactggcatg acactaccgt gcaaatgagc
     3241 gatttcaaaa acgagggtgt ggttgcttcc agtgcctggc cctatcaggc caacgccctg
     3301 aaagccgaag gccagcctgt tgctaccgtt ttcccgaagg agggtgttac cggttgggct
     3361 gataccacca tgctgcatag cgaagcgaaa catccggttt gcgcctacaa atggatgaac
     3421 tggtcattaa cgccaaaagt gcagggcgat gtggcggcct ggtttggctc gttaccggta
     3481 gtgccggaag ggtgtaaagc cagtccgtta ttaggcgaaa aaggttgtga aaccaacggt
     3541 tttaactatt tcgacaaaat cgccttctgg aaaacgccta tagcagaagg gggcaagttt
     3601 gttccctaca gtcgctggac gcaggattac attgccatta tgggcggtcg ctaacttcgc
     3661 tggggtgctt tatgacgtac gcagtggagt ttgacaacgt ctcgcggttg tacggtgacg
     3721 tgcgcgcagt agatggcgtc agtattgcga taaaagatgg tgagttcttc tctatgctgg
     3781 ggccgtccgg ctccggcaaa accacctgcc tgcgcctgat tgctggcttc gaacagcttt
     3841 ccggcggggc tatctctatc tttggtaaac ccgccagcaa tctgccaccg tgggagcggg
     3901 acgtgaatac tgtctttcag gactacgcgc tatttccgca tatgtcgatt cttgacaatg
     3961 tcgcctatgg gctgatggtc aaaggcgtga ataaaaagca gcggcacgca atggcgcaag
     4021 aggcgctgga gaaagtggcg ttggggtttg tacagcaacg taaaccgtca caactttctg
     4081 gtggtcagcg ccagcgggtt gctatcgcca gagcattggt gaatgaaccg cgcgtattgc
     4141 tgttggatga accgctcggc gcactggatc tcaaattgcg tgagcagatg cagctggaac
     4201 tgaaaaaact gcaacagtct ctcggtatca cttttatctt cgttacccac gatcagggcg
     4261 aagcgttatc gatgtccgat cgtgtggcgg ttttcaataa tggacgcatt gagcaggtcg
     4321 attccccgcg cgatctctat atgcgcccgc gcacgccgtt tgttgccggg ttcgttggta
     4381 catcgaatgt ttttgatgga ctgatggcag agaaactttg tggcatgacg ggaagcttcg
     4441 ccctgcgacc ggaacatatc cgcctcaaca cccctggtga actgcaggcc aatggcacga
     4501 tccaggcggt gcaatatcag ggcgcggcaa ctcgttttga actgaaattg aacggcggtg
     4561 aaaaactgct tgtgagtcag gccaatatga caggcgaaga actgcctgcc acgctcacgc
     4621 ccggacaaca ggtgatggtt tcctggtcgc gtgatgtgat ggtgccgctg gttgaggaga
     4681 ggtgaatggc gatgaatgta ttgcaatcac cttcacgtcc aggtctgggg ctgtttttac
     4741 tgctgcttgg cccgctaatg tggtttggca ttgtctattt cggctcgctg ctgacactgt
     4801 tatggcaggg attttatact tttgacgatt tcaccatgtc ggtaacgccg gaactgacgc
     4861 tggcgaatat ccgtgcgctg tttaatccgg cgaattacga catcattctc cgcacgctga
     4921 ctatggctgt ggcagtcact atcgccagcg ccattctggc ttttccaatg gcgtggtata
     4981 tggcgcgcta taccagcggg aaaatgaaag cgtttttata ttgcggtaat gttgccgatg
     5041 tgggcgagct acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg
     5101 gctcagtggt ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta
     5161 cctgcggtgg gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc
     5221 tatatctggt tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg
     5281 tcattgttgc aggcgtcggc tgatctcggc gcacgtccac gacaaacctt tcgctatgtg
     5341 gtgctgccgc tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcact
     5401 ctgggcgatt ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg
     5461 gtttattccc agcagggggc gattggcaat atgccgatgg cggcggcatt caccttggtg
     5521 ccgattattc tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca
     5581 ctctgaacgc gcaccgtttt tcctcaaact ggcggcctgg ggcggcgttg ttttcctaca
     5641 ttttcccatc ctgataatcg ccgcctatgc gtttaacact gaagatgcgg cgtttagttt
     5701 tccaccgcag ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct
     5761 tgatgccgtg acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt
     5821 agggacgctg gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc
     5881 gctgttactg ctgctgccca ttgcgctgcc gggcagcgtc actggtctgg cgttattaac
     5941 cgcctttaaa accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac
     6001 tttttgtgta gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag
     6061 tctggttgag gcgtcaatgg atcttggggc caatggctgg caaaccttcc gctacgtagt
     6121 gttgccgaat ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt
     6181 cgatgaaatc atcgttacga cctttacggc aggtcatgaa cgaacgttac tgttgtggtt
     6241 gctcaatcag cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt
     6301 tatgttggta acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa
     6361 tggtcaataa ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga
     6421 actggttagc ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt
     6481 actggaaatt gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc
     6541 agcatttgcc gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact
     6601 ggctgatgtt atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg
     6661 caaaccgctg catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt
     6721 tttcgcgggt gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca
     6781 tacttcgatg atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta
     6841 tccgctgatg atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt
     6901 gcttaaacca tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga
     6961 tatcttcccg gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc
     7021 gctgaccggt catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga
     7081 gcacatcatc agccatgccg cgtcgtccat taagcgtact catatggaac ttggtggcaa
     7141 agcgccagtg attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac
     7201 atttggctat tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa
     7261 aggcatttac gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg
     7321 tgcgccggat gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg
     7381 cgtcagcaag gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg
     7441 tgaaaagcgc aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca
     7501 ggacgatgcc atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga
     7561 caacgaagaa caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt
     7621 atggacgaaa gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac
     7681 ctgggtcaat acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc
     7741 tggttacggc aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt
     7801 catggttaaa cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag
     7861 gttctgagtt taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat
     7921 taggctaaat gtttgatggc gatttttcat cctgt
//
LOCUS       DAZSBF010000192         7944 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZSBF010000192 DAZSBF010000000
VERSION     DAZSBF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.195"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            111..443
                     /locus_tag="ACHXWD_003346"
     CDS             111..443
                     /locus_tag="ACHXWD_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR7622174.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="ACHXWD_003347"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="ACHXWD_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR7622175.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHXWD_003348"
     CDS             complement(2281..2598)
                     /gene="yegR"
                     /locus_tag="ACHXWD_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR7622176.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            3004..3903
                     /gene="yegS"
                     /locus_tag="ACHXWD_003349"
     CDS             3004..3903
                     /gene="yegS"
                     /locus_tag="ACHXWD_003349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR7622177.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(3985..4758)
                     /locus_tag="ACHXWD_003350"
     CDS             complement(3985..4758)
                     /locus_tag="ACHXWD_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR7622178.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="ACHXWD_003351"
     CDS             complement(4864..5904)
                     /gene="gatD"
                     /locus_tag="ACHXWD_003351"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR7622179.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(5952..7307)
                     /locus_tag="ACHXWD_003352"
     CDS             complement(5952..7307)
                     /locus_tag="ACHXWD_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR7622180.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="ACHXWD_003353"
     CDS             complement(7311..7595)
                     /gene="gatB"
                     /locus_tag="ACHXWD_003353"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR7622181.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="ACHXWD_003354"
     CDS             complement(7626..>7944)
                     /gene="gatA"
                     /locus_tag="ACHXWD_003354"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR7622182.1"
                     /translation="EFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADD
                     DNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLD
                     SDE"
BASE COUNT         2148 a         2036 c         1783 g         1977 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctagtgtt
     2641 taaatataat aatggcatga ttattataat tgaataggat tataataaat gttctgtaca
     2701 acatttccta cataagtagg aattacggac attgaggccc ttcagggtaa ctccatgggg
     2761 gctttaatat attatattga agatgccact gtttagttga atattaggta tatgctcttt
     2821 tttgaaattt atcggtggca gccgttagta ttcgctgtcc ccattgcaag ctcctggtgg
     2881 taaccactga atcctccata cttgaactga ctttttatcc tccgactttc atcctgttct
     2941 gactccacct tttgttttct gctctacact atctacagac caatcataaa ggcacatacg
     3001 atcatggcag aatttcccgc cagcttactg attcttaatg gcaaaagtac tgacaatcta
     3061 cccttgcgcg aagcaattat gctgttgcgt gaggaaggaa tgacgatcca tgtgcgggtc
     3121 acctgggaga aaggcgatgc cgcacgatat gtagaggagg cccggaagtt gggcgtcgca
     3181 acggtgattg ccggtggtgg cgatggcacc attaatgaag tttctacggc gttgattcag
     3241 tgtgaggggg atggcatacc cgcgctggga attttgccat taggaaccgc caatgatttt
     3301 gccaccagtg tagggattcc tgaggcactg gataaggcgc tgaaactggc aattgccggt
     3361 aacgccattg cgatagatat ggcgcaggtc aacaaacaaa cctgttttat taatatggcg
     3421 acaggcggat ttgggacgcg tattaccaca gaaacgccgg aaaaattaaa agccgcgctg
     3481 ggtggcgtct cttacatcat tcatggctta atgcgcatgg atactctgca accggaccgt
     3541 tgtgaaatcc gcggtgaaaa ctttcactgg caaggtgacg ccctggtcat tggtattggt
     3601 aacgggcgtc aggccggtgg cggtcagcaa ttgtgtccga acgcgttaat taacgatggc
     3661 ttgctgcaac tgcgcatttt taccggcgat gaaattcttc cggctctcgt atcaacctta
     3721 aaatctgacg aagataaccc gaatattatc gaaggcgctt cgtcgtggtt tgatatacaa
     3781 gccccacacg aaatcacttt taatcttgat ggcgaaccgt tgagcggaca aaactttcat
     3841 attgaaatac ttccggcggc gttgcgttgt cgattaccac cagattgtct attattgcgt
     3901 taatcaaact attctctctt gaaccaggga gaatccgaaa tgccggatgc ggtgtaaata
     3961 ccttatccgg catacagaac aatactacac cgtaattaat tttacccgtg tcttctgcaa
     4021 tgccagttta tccacctctg atacagcatc atcggtaata ataatgtcaa ttttttcgat
     4081 tggcagtacc tgattaaaac cgcgacggtt aaatttcgat gaatcgagaa cggcaatcac
     4141 tttattagcg gcggtgacca tcgcaccgct aatcgaataa ccttcattaa aggtcgtaat
     4201 accattaacc gcatcaatac catcagcacc gacaaacatt aaatcggcat taatatcctg
     4261 caatgaacgc tcggcaatgg aaccatgcat cgagcgcgtt ttatggcgga ccgtgccacc
     4321 acagacgacc agagtaatgt ctttattttc ggaaagggca aacgctgccg ggagactgtt
     4381 ggtaatcacc gtgatatttt tagcggtcat taatccttcg gcgataagca ttgtcgtact
     4441 tccgctgtcg agaataacgg tcatcccttc atggatcatt ttgactgccg cctgcgctat
     4501 tctgtttttt ggcgcgctgg cgagctgaaa tcgctcttta aacccgactt cctgggtctc
     4561 ggtttcacta ttaccggaca ttattttcgc cgcaccgcca tgaaagcgcg taaccacgcc
     4621 tttttgttcg agaaagcgca aatcggcacg gattgtcgct tccgaggcag caaatactcc
     4681 cgccagatcc tgaacaagca cggttccctg ttcattcact aattggatga tcttattcct
     4741 tcgctcgaat gaattcatcg tcatttaccc atattcaatt gtggctagtg taaacgaagg
     4801 gggaccattg gtgaataatc tgttttgcac aataccgggc cagcatcacg ctggcccgcg
     4861 gtttcatgga atgagcaaca ctttgcccgg catggcatta cgagcgatgt cacgcaccgc
     4921 ctgggtgaag ctttcaaagc ttccacggtg agcgattaat ggctccaggc ttaacttacg
     4981 ttctgtcagc aaccggctcg ccgtttccca ctcctgcccc ggccaagggc tggaatagtt
     5041 catccaactg ccgataaccg tcagctcttt acgcaatatt ttgccaaacg ttgccgatgt
     5101 taaatgcaga tcctgatgca acgtgcccac cagcgccagt tgggcatgag gaccggcaat
     5161 ctctaccgcc agttcgacag tttgcggtac gccagccgtc tcgaggataa gctgattaaa
     5221 gcgcagttcg cgtaaaacgc tctgcatttg cggcgcgctc atttcgctac tgttaaatgt
     5281 ttgcatcgca ccgaaagatt ttgccaatgc cagtttttct gagctaatgt cgatcgccgt
     5341 cacactcttt gctcccagcg cgacagcgca ctgaatagcc agcaggccaa tggttccggc
     5401 accaataata ataacgtttt tattctcaca accttgcgct aaatgaaaag catgcaggcc
     5461 aacggtaatc ggctcaataa aagccccatc ctcaataggc atatccgtgg gtagagcaaa
     5521 gacatttttt cgcttaacga caatatattc agcaaatcca ccatcacgcc gcgagccaat
     5581 aaaatcatat tttgcgcact gggaataaaa ccctttcaaa cactctggat aagtaaaaca
     5641 gggtaataac ggcacacagg caaccgcatc gccagggtgt aaatcatcaa caccggatcc
     5701 caccgcatca atatagccgc taaattcatg gcctaacgtt attggataat aatgtgcacc
     5761 atttttaaat atcctgggta aatcggaacc acataagccc gagctggcaa tttttacccg
     5821 cacctcatcc tgatgtttaa tttcaggaat gacgctttct gcaacgcgca cgataccatc
     5881 agtatcattc accattgatt tcataaaaac tcctgattgt taagggggat aacctccccc
     5941 tcaggtaaaa attattctgc gagaacggtt ttctcttgtt taataaaacc acgcgctcta
     6001 cgccaggtca tgaaaatacc ggtaagataa attgcgccga taataatgaa accgggaata
     6061 ttttgcgtgg agaaaacctg aatcagtaac caggtaatgg gagaaccgcc ctgatccatt
     6121 gaagccacca tacccccggc ttttaacgcc ccagcattag ccgccagttg ggtgtgcaaa
     6181 ccaatagttt gcgtcgcgat ccacagggtg atgctcataa tgatgacacc cgagattaag
     6241 gtgcggaaca gatttccacg atgcaccgcg accgccatcg ccacgaagaa gccgatggtg
     6301 gcaagatcgc caaacggcag tacctgatta cccggaacac aaacagcaat taaaatggtg
     6361 agcgggataa aaatcaggct tgccgatacc accgccgtat gccccagcag taatgcggga
     6421 tccaggccaa tcaggaactc ctgaccgccg aacttcgcct gtaaacgact acgggcctgc
     6481 ttagcgatgg gcgttaaacc atccatgatg ggtttaatca cccgtggcat cagcagcata
     6541 actgccgccg tttttaccgc cagctgcaat acacctttga catcgtaacc ggcgaggatg
     6601 ccgataatca gccccatcac aaaacccacg gtgacaggct cgccaaatgg accaaatttg
     6661 cgctgaatat cgtcggcgct aaatttaatt cggttaacgc ctgggatttt ctcgatgata
     6721 gcatcgacca gcaccgcaat cggccccata tacgccgacg taccgtgcgg aatagcaata
     6781 ccttccagct caaagaaatt tcgggtatcg cgggcaaacc agtcgccgag cttataaaca
     6841 aacgccgcgt gaattaccac acctgccatc cctatcatcc atgaaccggt tgccagatgc
     6901 agcaacgcgc cggtgaaggt catatgccag atattccaga tatcaacatt taccacccgc
     6961 gtcatacggg tcagtaacat cgccacgtta accagaatcg caatcggaat cgccaccagc
     7021 gcaatttgcg acgcccaggt cattggtgaa gagcccggcc agccgacatc gaccacatgc
     7081 agattcaggt cgaaattttc cgccatcgct ttcgccgccg gaccaatgga atccagcatt
     7141 aagccaatca caaggccaat gccaacaaag ccaatcccga tatgcagacc cgctttaaag
     7201 caatcgcctg ccttcatgcc taatatttta gaaaaaataa tgatgacaat cggaagcatc
     7261 accgtagggc cgaggtcgag aatataacgc atgacttctg aaaacatagg tcacccctgt
     7321 aagatagtca gaattttatt ttgtaatgct tcgataccga caccagaaat aaaaggcatg
     7381 ccgtgaacta acggaatatc gccaaaacta cgatccactt tggcagtggt gcatatcaaa
     7441 tgcacaccat ccatataggt ttctatttca ttaacccgac actggattaa ttcaacagga
     7501 atactatgat tctgacacaa ctctttaatt tcttccgccg ccatcgtaga ggtcgcaacc
     7561 gcgcctccgc aagcgacaat aatcttgcgt ttcataaata gtccttttta ttgttacaga
     7621 gggatttatt cgtctgaatc taaaacatac tttgtgaagt attcctttaa ctgggtttca
     7681 ggaagagtga ttagtgtctc gacgatatcg ggctgttgta acttgccaaa taaacagcgt
     7741 aaaagtttca attgctgctg cggattctcc acaatcaacg caataaccaa cgatacctcc
     7801 acgtcgttat catcatccgc ttgctgaaaa tgaactttat ttgttggcct taacagataa
     7861 atggctgacg acttagcatg aatcgcctca caatgcggta ttgcaatggc gtgctgctca
     7921 agcattatcc cggtagggaa ttct
//
LOCUS       DAZSBF010000193         7818 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZSBF010000193 DAZSBF010000000
VERSION     DAZSBF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.196"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="ACHXWD_003355"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="ACHXWD_003355"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR7622183.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="ACHXWD_003356"
     CDS             complement(2362..2802)
                     /locus_tag="ACHXWD_003356"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR7622184.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHXWD_003357"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="ACHXWD_003357"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR7622185.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="ACHXWD_003358"
     CDS             3520..4218
                     /locus_tag="ACHXWD_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR7622186.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="ACHXWD_003359"
     CDS             4447..5655
                     /locus_tag="ACHXWD_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7622187.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="ACHXWD_003360"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="ACHXWD_003360"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR7622188.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
BASE COUNT         1790 a         1918 c         2076 g         2034 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttc
//
LOCUS       DAZSBF010000194         7782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZSBF010000194 DAZSBF010000000
VERSION     DAZSBF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.197"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(362..451)
                     /locus_tag="ACHXWD_003361"
     tRNA            complement(362..451)
                     /locus_tag="ACHXWD_003361"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(415..417),aa:Ser,seq:cga)
     gene            545..1342
                     /gene="mtfA"
                     /locus_tag="ACHXWD_003362"
     CDS             545..1342
                     /gene="mtfA"
                     /locus_tag="ACHXWD_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR7622189.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            1443..1518
                     /locus_tag="ACHXWD_003363"
     tRNA            1443..1518
                     /locus_tag="ACHXWD_003363"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1476..1478,aa:Asn,seq:gtt)
     gene            1832..7782
                     /gene="yeeJ"
                     /locus_tag="ACHXWD_003364"
                     /pseudo
     CDS             1832..7782
                     /gene="yeeJ"
                     /locus_tag="ACHXWD_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         2175 a         1918 c         1886 g         1803 t
ORIGIN      
        1 ctgggaaatt caacaactct tggaaattat ggactttgtc ccgcggcttt aatctgacgg
       61 ccgcgttcct tttttgactc aattattgac cccttctcta cgcaacttca gttcccacca
      121 ccaactttgc ggcagtttca taggatgaat gtctaaaaga ataatggtga gcgataagaa
      181 aacgactgaa taactgcaca ttttcgctcg aaagcttcct gtcagatcca tagcgaatca
      241 agtgctgaat gtcacagtat cgaacagaaa acagtgacga tctaaccctt caagaatatt
      301 ctacaattgt tctgtttagg aaaagcaagg cgggaagtca ggagataagt cattgataaa
      361 gtggcggaga gagggggatt tgaacccccg gtagagttgc ccctactccg gttttcgaga
      421 ccggtccgtt cagccgctcc ggcatctctc cgttcagatg gttgccatga tgccaggaaa
      481 tttggcattt taacagtccc tgttcgtgca attttgttca agtgacgagt ttgcgagcaa
      541 aacgatgatt aagtggccct ggaaagtaca agaatcagca catcaaactg cccttccctg
      601 gcaggaagca ctatcgatcc cccttttaac gggtctgacg gaacaggaac aaagcaaatt
      661 agtcactctt gccgaacgtt ttttacagca aaagcggctt gttcctttac agggctttga
      721 actggattca ttaagaagct gccggatagc acttctattt tgcctacccg ttctggagtt
      781 aggactggaa tggctggatg gttttcatga agtcttaatt tatcctgcgc catttgtggt
      841 cgatgatgaa tgggaagacg atatcggtct ggtgcataac caacgtattg ttcagtcagg
      901 tcagagctgg cagcaagggc ctatcgtttt gaactggttg gatatacaag attcttttga
      961 tgcatctggt tttaacctga ttattcatga agtcgctcat aagctggaca cccgtaacgg
     1021 cgatcgcgcc agcggagttc cctttattcc gttgcgtgag gttgctggct gggaacacga
     1081 tcttcatgct gcaatgaaca acattcagga agaaatcgaa ttggttggcg agaatgcggc
     1141 gagcattgat gcttatgcag ccagcgatcc tgctgaatgt tttgccgtac tttctgaata
     1201 tttctttagc gccccagaac tttttgctcc tcgtttccct tcattgtggc aacgtttctg
     1261 ccaattttat caacaagatc ctttgcagag actgcatcac gctaatgata cagactcgtt
     1321 ttcggcgacg aatgttcatt aattaacaac tttgcagatt aattaaccaa ttgaaatgac
     1381 ttatgaaatt tagtgttgac agacaaggta ccgctaagta atatgtgccc cgttcacacg
     1441 attcctctgt agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga
     1501 gtccagtcag aggagccaaa ttctaaaaat tcgctttttt agcgcaatgt cactgacctt
     1561 agttgaacat tgttttttaa cggatagcgg gtttttaaca ccttaagcgc cctcgacctt
     1621 tatggttgag ggcgttttgc tatgaacgcc atcaccattt tcccctcgat tataaaactt
     1681 gagttattca gtagtctccc ctcttgcaac tcacacccaa aactgcctaa cgaaaagtta
     1741 ttaattttca atcgtattgc tatcagtatt tacatttttt cgctgtgcta gaaagggcgc
     1801 atttatgtta gctcgttcag ggaaggtaag catggctacg aagaagagaa gtggagaaga
     1861 aataaatgac cgacaaatat tatgcgggat gggaattaaa ctacgccgct taactgcggg
     1921 tatctgtctg ataactcaac ttgcgttccc tatggctgcg gcagcacaag gtgtggtaaa
     1981 cgccgcaacc caacaaccag ttcctgcaca aattgccatt gcaaatgcca atacggtgcc
     2041 ctacaccctt ggagcgctgg aatcggccca aagcgttgcc gaacgtttcg gtatttcggt
     2101 ggctgagtta cgcaaactca accagtttcg tacgtttgct cgaggttttg ataatgtccg
     2161 ccagggtgat gaactggatg tcccggcaca agttagtgaa aaaaaattaa ccccgccgcc
     2221 gggtaatagc agtgacaacc tcgagcaaca gatagccagt acttcacagc aaatcgggtc
     2281 tctgctcgcc gaagatatga acagcgagca agcggcaaat atggcgcgtg gatgggcctc
     2341 ttctcaggct tcaggcgcaa tgacagactg gttaagccgc ttcggtaccg caagaatcac
     2401 gctgggcgtg gatgaagatt ttagcctgaa gaactcccag ttcgattttc tccatccgtg
     2461 gtatgaaacg cctgataatc tctttttcag tcagcatact ctccatcgta ctgacgagcg
     2521 tacgcagatt aacaacggct taggttggcg tcatttcact cccacatgga tgtcgggcat
     2581 caacttcttt ttcgaccacg atcttagccg ttaccactcc cgcgtcggca ttggcgcgga
     2641 gtactggcgc gactatctaa aattaagcag taacggctat ttgcgactga ccaactggcg
     2701 cagcgcacct gaactggaca acgattataa agcacgcccg gccaatggct gggatgtacg
     2761 cgcagaaagc tggctacccg cctggccgca ccttggcggt aaactggtct atgaacagta
     2821 ttatggcgat taagtggccc tgttcgataa agacgatcgg caaagtaatc ctcatgccat
     2881 aaccgctgga cttaactata cccccttccc gctgatgacc ttcagcgcgg agcaacgcca
     2941 gggtaaacag ggcgaaaatg acacccgttt tgccgtcgat tttacctggc aacctggcag
     3001 cgcaatgcag aaacagcttg acccgaatga agtcgctgca cggcgtagcc ttgcaggcag
     3061 ccgttatgat ctggtggatc gcaacaacaa tatcgttctg gaatatcgca aaaaagaact
     3121 ggttcgccta accctgacag accccgtgac agggaagtca ggagaagtga aatcactggt
     3181 ttcgtcgcta caaaccaaat atgccctgaa aggctataac gtcgaagcca ccgcactgga
     3241 agctgccggt ggcaaagtgg tcacaacggg taaagatatt ctagttaccc tgccggctta
     3301 ccggttcacc agtacgccag aaaccgataa cacctggccg attgaagtca ccgccgaaga
     3361 tgtcaaaggc aatttgtcga atcgtgaaca gagcatggtg gtcgttcagg cacctacgct
     3421 aagccagaaa gattcctcgg tatcgttaag tacccaaaca ttgaacgcgg attcccattc
     3481 aaccgccaca ctgactttta ttgcgcatga tgcagcaggt aatcctgttg tcgggctggt
     3541 gctctcgacg cgtcacgaag gtgttcagga catcaccctt tctgactgga aagataatgg
     3601 tgacggaagc tatacccaga tcctgaccac aggagcgatg tctggcacgc tgacgctgat
     3661 gccacagctg aatggtgtgg atgcggctaa agcccccgcc gtggtgaata tcatttctgt
     3721 ttcgtcatcc cgaactcact cgtcaattaa gattgataag gaccgttatc tctccggcaa
     3781 tcctatcgag gtgacggtag aactgagaga tgaaaatgac aaacctgtta aggaacaaaa
     3841 acagcaactg aataacgcag tcagcatcga caacgtgaaa ccaggagtca ctacagactg
     3901 gaaagaaacc gcagatggcg tctataaggc gacctatacc gcctatacca aaggcagtgg
     3961 acttactgcg aagctattgg aatgaagatt tgcataccgc tggatttatc atcgacgcca
     4021 acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgta ctcgccaatg
     4081 agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac ccaatcaatg
     4141 atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac aatcaaaaca
     4201 ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt aagcaggaag
     4261 acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc gtcagttttg
     4321 tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa gtggttgctg
     4381 acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc aacctgctca
     4441 atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc caaaccgaag
     4501 tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat ggtgattata
     4561 gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat tttatcggtg
     4621 atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc gtcaccaaca
     4681 cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca ctaaaagata
     4741 aagaaatcac cttctctgtg ccaaacgacg tcgcaagtaa gttctcgatt agcaacggag
     4801 gaaaaggcat gacggatagt aacggggttg caatcgcctc cctgaccggc acgttagcgg
     4861 gcacgcatat gatcatggct cgtctggcta acagcaatgt cagcgatgca cagccaatga
     4921 cgtttgtggc ggataaagac agagcggttg tcgttttgca aacatcgaaa gcggaaatca
     4981 ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatccg tcgaatcatc
     5041 cggtggcggg gataacggta aacttcacca tgccacagga cgttgcggca aactttaccc
     5101 ttgaaaataa cggtattgcc atcactcagg ccaatgggga agcgcatgtc acgctgaaag
     5161 gtaaaaaagc gggcacgcat acggttaccg caacgctggg taataacaat accagtgatt
     5221 cgcagccggt aacatttgtg gcggacaaag cctcggctca ggttgtcctg cagatatcaa
     5281 aagatgagat cacaggtaat ggcgtcgata gcgcaacgct aactgcaacg gttaaagatc
     5341 agttcgacaa tgaggtgaat aatcttccgg taacattcag ctcagcctct tcaggactca
     5401 ccctgacccc gggagtaagt aataccaatg agtctggcat cgcgcaggcc actctcgcag
     5461 gcgttgcctt tggtgagcag acggtcactg catcactggc taataatggt gccagcgaca
     5521 acaaaactgt gcattttatt ggcgacacag cggcggcaaa aattatcgag ttgacgcctg
     5581 tcccagacag cataatcgcc ggtaccccgc agaacagctc cggcagcgcc atcaccgcca
     5641 cagtcgttga taataatggc tttccggtga aaggtgtgac tgtgaacttc accagcagaa
     5701 caaactctgc cgaaatgacg aatggcggcc aagccgtaac gaacgaacag ggtaaggcta
     5761 ccgtcactta taccaatacc cgctcctcga tagaatcagg agcgagaccg gataccgttg
     5821 aggccagtct ggaaaatggt agctccacgc ttagcacatc aattaatgtc aacgctgatg
     5881 cgtctacggc acatctcacc tggctacagg cactttttga tacagtctcc gcaggcgaga
     5941 caaccagtct gtatattgag gtgaaggata attacggcaa cggtgtcccc cagcatcagg
     6001 tgaccctcag cgtttcacca agtgaaggcg tgacccccag taataacgct atatatacta
     6061 ccaaccacga cggcaatttt tacgcaagct ttaccgctac aaaagccggg gtttatcaag
     6121 tgacggcaac cctcgaaaat ggcgattcga tgcaacaaac agtgacctat gtgccgaacg
     6181 tcacgaatgc cgaaatcacg ctggcaacct cgaaggatcc ggtgattgcc gacaataacg
     6241 atctcacgac actaacagca acagtcgctg atacagaggg caatgcgata gccaacactg
     6301 aagtaacatt tactctgccg gaagatgtta aggcgaactt cacgctgagc gatggcggta
     6361 aagcgattac tgatgctgaa ggcaaagcga aagtcacgct gaaaggtaca aaagcaggcg
     6421 ctcatactgt tacagcatcg ataactggcg gtaagagtga gcagttggtg gtgaacttta
     6481 ctgcggatac gctcactgcg caggttaatc ttaacgttac cgaggacaat tttatcgcta
     6541 ataacgtcgg gatgaccagg ctgcaggcaa cagtgactga tggaaacggc aacccgttag
     6601 ccaatgaggc ggtgacattc acgctaccgg cagatgtgag cgcaagcttt actctcggac
     6661 aaggcggttc cgccattact gacatcaacg gcaaggctga agttacactg agcggtacaa
     6721 aatccggcac ctaccccgtg acagttagcg tgaacaatta tggtgtcagt gatacgaaac
     6781 aggtgacttt gattgccgat gctggtaccg caacactagc ctccttaacc tctgtatact
     6841 cattcgtcgt cagcacgacc gaaggcgcga ccatgactgc aagcgtcact gacgctaacg
     6901 gcaacccggt agaaggcata aaagttaatt tccgcggaac ctccgtcacg ctaagcagca
     6961 ccagcgttga aacggatgat cggggtttca ctgaaattct tgtgacaagc accgaagtcg
     7021 gactgaaaac agtttcagcc tctctggcag ataaacctac tgaagtcata tcgcgattac
     7081 tgaatgcaaa agcagatatt aattctgcaa cgattaccag tctggagata cctgaaggtc
     7141 aggtcatggt cgcacaagac gtagcagtta aagctcacgt caacgaccag tttggcaacc
     7201 cgattcttaa tgaatctgta acattcagtg cagagccacc agagcacatg accatcagcc
     7261 aaaatattgt ctctactgat acgcatggta tagccgaggt ctccatgacg cccgaaagaa
     7321 acggttcgta tatggtgaaa gcatccctgg cgaatggagc ctcacttgag aaacaactgg
     7381 aggctattga tgaaaaactg acactctcgg cgtccagccc gcttatcggt gtcaattccc
     7441 caacaggtgc aactctgacg gcaacgctaa cttctgcaaa tggcactcca gtggagggtc
     7501 aggtcatcaa ctttagcgta acgccagaag gtgcgacgtt aagtggcgga aaagtgagaa
     7561 ctaactcttc tggtcaggct ccagtcgttc tgaccagcaa taaagtcggt acatatacgg
     7621 tgactgcatc gttccataac ggcgtaacaa tacagacaca gacaaccgtg aaagtcactg
     7681 gcaactcaag caccgcccat gttactagct ttatcgctga tccatcgact atcgccgcca
     7741 ccaacagtga tttaagtacc ttaaaggcaa cggttgagga tg
//
LOCUS       DAZSBF010000195         7776 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZSBF010000195 DAZSBF010000000
VERSION     DAZSBF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.198"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHXWD_003365"
     CDS             complement(<1..1780)
                     /locus_tag="ACHXWD_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR7622190.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPEWLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHXWD_003366"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHXWD_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR7622191.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHXWD_003367"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHXWD_003367"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR7622192.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHXWD_003368"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHXWD_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR7622193.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHXWD_003369"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHXWD_003369"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR7622194.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHXWD_003370"
     CDS             complement(5968..7005)
                     /locus_tag="ACHXWD_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR7622195.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHXWD_003371"
     CDS             7338..>7776
                     /gene="yhhY"
                     /locus_tag="ACHXWD_003371"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR7622196.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1977 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc attcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaag ccagcacccg
     7141 gctggctaag taatactgga agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag
     7201 ggtcttcctg atcgcgagac aataataatc attctcattc gcacttgtcc aacacttttt
     7261 gcaaaaaaaa tgcatttgac tcgcatttga aagtcaatga tgttgaaagg gacatttacc
     7321 ccaaagagga caaaggaatg agtgagatag taatacgcca cgcagaaaca cgggattacg
     7381 aggccatcag gcagattcac gcccagccgg aggtgtattg caacacacta caggtgcctc
     7441 atccttccga tcatatgtgg caggagcgac tcgccgatcg tcccggcatc aagcaactcg
     7501 tcgcctgtat tgatggagac gtcgtgggcc atctcaccat tgacgtgcaa cagcacccac
     7561 gccgcagtca tgttgccgat tttggtatct gtgtcgactc tcgttggaag aaccgcggcg
     7621 tcgccagcgc cctgatgcga gagatgattg aaatgtgcga caactggttg cgggtagatc
     7681 gcattgaact aaccgtgttt gtcgataacg ccccggcaat taaggtctat aaaaaatacg
     7741 gctttgaaat tgaagggact ggtaagaagt acgcat
//
LOCUS       DAZSBF010000196         7732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZSBF010000196 DAZSBF010000000
VERSION     DAZSBF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.199"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            8..505
                     /locus_tag="ACHXWD_003372"
     CDS             8..505
                     /locus_tag="ACHXWD_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR7622197.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYILYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            502..972
                     /locus_tag="ACHXWD_003373"
     CDS             502..972
                     /locus_tag="ACHXWD_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR7622198.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1061..1573)
                     /locus_tag="ACHXWD_003374"
     CDS             complement(1061..1573)
                     /locus_tag="ACHXWD_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR7622199.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSHSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1768..2532
                     /locus_tag="ACHXWD_003375"
     CDS             1768..2532
                     /locus_tag="ACHXWD_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622200.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2483..3886
                     /locus_tag="ACHXWD_003376"
     CDS             2483..3886
                     /locus_tag="ACHXWD_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR7622201.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3886..5352
                     /locus_tag="ACHXWD_003377"
                     /pseudo
     CDS             3886..5352
                     /locus_tag="ACHXWD_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5243..5986
                     /locus_tag="ACHXWD_003378"
     CDS             5243..5986
                     /locus_tag="ACHXWD_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR7622202.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5990..7210
                     /locus_tag="ACHXWD_003379"
     CDS             5990..7210
                     /locus_tag="ACHXWD_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR7622203.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7214..7720
                     /locus_tag="ACHXWD_003380"
     CDS             7214..7720
                     /locus_tag="ACHXWD_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622204.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2059 a         1842 c         2105 g         1726 t
ORIGIN      
        1 gtgaataatg tcgccatcat tacgcaaggc tgttgcagtt gctattggtg gcggggctgt
       61 tgctatagca tctgtgttaa tcactggccc aggtggtaac gatggtctgg aaggtgtcag
      121 ctacatacta tacaaagata tcattggtgt atggactgta tgtcacggac acaccggaaa
      181 agacatcatg cctggtaaaa cgtataccga agcagaatgc aaagctctcc tgaataaaga
      241 ccttgccact gtcgccagac aaattaaccc gtacatcaaa gtcgatatac cggaaacaac
      301 gcgcggcgct ctttactcgt tcgtttacaa cgtgggcgct ggcaatttca gaacatcgac
      361 gcttcttcgc aaaataaacc agggctatat caaaggcgca tgtgaccagc tatgtcgctg
      421 gacatatgct ggcggtaagc aatggaaagg gctgatgacc cgtcgtgaga ttgagcgtga
      481 agtctgtttg tgggggcagc aatgagcaga gtcaccgcga ttatctccgc tctggttatc
      541 tgcatcatcg tccgcctgtc atgggctgtt aatcattacc gtgataacgc cattacctac
      601 aaagagcagc gcgacaaaaa cgcaagagaa ctgaagctgg cgaacgcaac cattactgac
      661 atgcagcagc gccagcgtga tgctgatgca ctcgatgcta aatacacgaa ggagttagct
      721 gatgcgaaag ctgaaaatga tgctcttcgg cgcaagcttg ataatggtgg tcgggtgctc
      781 gtcaaaggaa aatgccctgt gccatcctca gccgaaacct ccagcgcctc cggcatgggc
      841 aatgatgcca ccgtcgaact ctctccagtt gctggacgaa acgttctcgg tatccgggac
      901 ggaatcatca gcgaccagac agcattgaga atgcttcagg agtacatcag gatccaatgc
      961 cttggggggt agcggtaatt ttactcatta tccttcaaat cagattctgt tgtcagagga
     1021 atgggggaag ctggattcgc aagagctttt tatgggcagt ttagctaaaa aaaggcaaca
     1081 cgaagctaaa actaccaaca taaagtgctg accagctagc agctgaaatc agactcactg
     1141 tatacacttt cactggatga agttgcaaca ttcctgccac caaaggagca atataacgca
     1201 gcacggcgat aaagcgcgaa gtgaaaagta ttgacacaga gttattttgc aactgaaggc
     1261 gaacccgttc tattgtattg gaatggcttg atattataca tgcaagttga gggatatgcc
     1321 caattattat accaagatgg taattaacga tagtaccgca ccatgcgcca ctcatgaccg
     1381 tgattccagc ttcccatggt gaaagagtcg tcttgctgac tgtaattaca gtggccatca
     1441 taattgatgc tggtggcaat atcgccgaaa tgagtaaagt ggattttgtg aacgctatta
     1501 taaaaagaag cccccatagg cgatatggat gaagtacaaa atagttcatc aaagcattta
     1561 tccactccat caatgtcttt ccttctgtga atttttcttt gctatttaac cgtgatacga
     1621 cttaatttag gcttaaacac cttacattta caaggtactc ctgtgggggg ccctgccacg
     1681 gagcgtcgca ggagtgagtg atgaggaaga attaaagcaa cctattgtga caggcagcga
     1741 taacagtaaa tcacaggaag cataatcatg gcaaaaccgg actgggaggc catcgaatcg
     1801 gcataccggg ccggagtcct tagcctccgt gatataggcg agaaatacgg cgttacagaa
     1861 ggggctatca ggaagagggc caaaaagctt ggttgggcac gcagtggcgg tacgcaggtt
     1921 tgcaaaaatg gtacgcaaaa aaggaaagtg cgtaccagca gaaagcctgc cattactggc
     1981 cttacacaaa aaagtacgca actaaaaaca gaatctacac cggatacgaa accgatacgc
     2041 ggaatgcgta ccgatccccc gactaaccca ttccaacccg gtaaccagca ggcattaaaa
     2101 cacggtggtt atgcccgtcg ccttctgctc aaagatgagg tgatagagga cgctaaagcg
     2161 ttgacgctcg aggacgaatt atttcgcctt cgtgctaaca accttgtcgc tgcagagaat
     2221 attggtcggt ggttggtgtc gctggaagat gctaatgggg accaggaaag gaagatgctg
     2281 attgaaaata tcagcgccgc cgagaaagca atgatgcgca atacagttcg tattgagtcc
     2341 atcgttggca cgcttgcgac ggtaggaaaa atatttgctg atacagccta ccgcaaggcc
     2401 gccactgata aggtgtctct ggaggctgat cgtcttcgcc gtgatgcagg tattgatgat
     2461 ggcaatggag agcgtgacct caatgacttc tactctgaca tccaaaccga cgctgaatcc
     2521 ggctttacgt agtttctgga ctatgcgggc acgtaacaaa gtgctttatg gtggtcggtc
     2581 atcgtcaaaa tcatgggatg ccgctggcat tgccatattt ctgtcgaata aatacaccct
     2641 gcgtttttgt tgtgcccgtc agatccagaa taaaatcgaa gagtcggtgt ataccctgct
     2701 caaaattcag atagacaggt ttggtctgcg gcaccgtttc cgtattctga acaacaaaat
     2761 cattaaccgg gttactggct cggaatttgt tttttatgga ttatggcgca acatcgaaga
     2821 aattaagtca ctggagggga tcgatgtgtt gtggctggaa gaagcccacg cactgacgga
     2881 ataccagtgg aaaattctgg agccaacgat ccgtaaagag ggttcggaat gctggttcat
     2941 attcaacccc ggacttgtta ctgatttcgt ctggcgcaac ttcgttgttg atccgcccga
     3001 aggcactctc atccgcaaaa ttaactatga cgaaaatccg tttctgtctg acaccatgct
     3061 taaggttatc gacgcggcgc gacgccgtga tccggatggt tttaaacatg tgtatgaggg
     3121 cgttccggag tctgatgatg atgcggcaat catcaaactg tcttggatag aagccgcagt
     3181 ggatgcgcac aaaacgttaa atttcgaacc cagtggaaga aagcgtattg gctttgacgt
     3241 ggctgacagt ggtacagata agtgcgctaa cgtttaccgt cacggatccg ttgttttctg
     3301 ggccgacgaa tggaaggcca aagaagatga attactgaag agctgccagc gtacttatca
     3361 ggcggcgctg gagcgtgaag cagatattgt ttacgactct atcggtgttg gtgcgtctgc
     3421 cggtgctaaa ttctctgaaa ttaacgctga ccggaagagc gagaacgcat acgcgcgacg
     3481 tgtgaattac cagaggttta acgccggtgc tggtgtgcat gagccagatg acgagtacaa
     3541 cggcatcccc aacaaagact ttttcgcaaa tcttaaggct caggcatggt ggctggtggc
     3601 tgaccgtttc agaaatacgt ttaacgccat taacaacgga gaacagtatc ctgtggatga
     3661 gctgatcagc atagattctc gttgtccgtt gcttgaaaag ctgaaactgg aactgacaac
     3721 acctcatcgt gatttcgacc gtaacggacg tgtgatggtc gaaagtaaaa aagacctcgc
     3781 aaaacgcgag ataccgtcac caaacgttgc tgatgcattc attatggcct tcgcgccaat
     3841 cgatacatcg ctggatatct gggaacagct ggggagacag gcctgatggc acgaaacaaa
     3901 caagccctgc ggcgaactgt gcaggccaca gctgatggtt atgagaattt tattgcccgc
     3961 gtagggatgc agacacctaa ccagcactca gcatccacct accgggctaa tttcaccagt
     4021 cgtaaccgca tgctggtgga atggtcctat cgttcatcct agatcatcgg cgaagcagtc
     4081 gatgctatcc cggatgatat gacccgcaaa ggcattcgca tcacttcgga aattgatgca
     4141 aaagatcgtg gcattctcga atcacaactg gatgagttgc aaatctggga tgcgctgaat
     4201 gacgtgctga aatggtcgcg cctctacggc ggcgcggtgg gtttcatcat gattgagggg
     4261 taggcaccaa tgaccccgct gcgacccgaa accatcggta agggcaagtt taaggggatt
     4321 ctcccgctcg accgctggat ggtcgacccg gcactgaccc gccgcattaa agatatgggg
     4381 ccggacctgg gtaaacctga gttttacgat gtggtgacca cagcaacggg aattcctgcc
     4441 tggcgcattc atcacagtcg cctgattcgc tttgatggcg tcacgttgcc atttcagcag
     4501 aagatgaccg agaacgaatg gggaatgtcg gttgtagagc gtatctggga tcgtcttacc
     4561 gcgttcgaca gcgctactgt cggcgcggcg cagctggtct acaaggcgca tttgcgtacc
     4621 tacagcgtgg agaagctacg cgagcttatc gcacttggtg gtcctgcgta tgaagcgttg
     4681 ctgaagaata tcgacctgat tcgacagttc cagagtaatg aaggcatgac gctcatggac
     4741 tcgcgggata agtttgaaac ccatcagtac agcttcagtg gtctggatga catcctttcg
     4801 cagtttgtag aacagattag tggcgctgtt ggtatcccac tggtgcggtt gttcggacag
     4861 tccccgaaag gattttctac cggtgatgca gaccttgcca actattacga tcgcatcagt
     4921 tcgttgcagg agaggcgttt acgtcttccg gtgcggcgga tactggacat catgcatcgt
     4981 tcggaacttg gcaagccgct gccggacgat ttcacgtttg agtttaaccc gctctggcaa
     5041 atgtctgatg tcgatcgctc aacggtggcg ttaaacacca ccaacgcaat cagtacggcg
     5101 ctgggtgatg gtctgatgac actgaaagcc gctatgactg atttgcgaga aaattctgac
     5161 gtaaccggca tcggggcatc cattaccgac gaggacatcg agaatgccga agatgaagcg
     5221 ccgcccggca tcggcgaacc tgatgacgaa ccgcaggagc cgtcaggcgg aaatccggta
     5281 tcgaaccagc ctacgcagga tagcgagggc ggtcggagac atcgtaaatg gtcgctacga
     5341 tggttcaaat gacagtatca cggaaattat tgcggcgctg gagcgttaca gtgaaatcat
     5401 caccccctgg gcgacaaagg tcgcggaaaa ctttactgcg gacctaaccc ggcagaacga
     5461 gaaagtttgg cggcaacaca gcaagaacat cagtcgcgag ctccgcaatc ttgtggaaag
     5521 cgctcctgtg ggccaggtga tgcaatccat catcgccgaa caggtcaagt acatcaaatc
     5581 tctgcctctc gaggccgcag acagggtgta cgacatccag aacaaagcga tagaggctgt
     5641 tgtcaccggt ggtcgggcgg agcagtttgc taaagagatt gcatctaccg gcgatgttgc
     5701 taagtctagg gccgatctga ttgcccgaac ggaactggga agagcaacgg gcgcgctgga
     5761 tatggcccga gcgatagcta ttggctcgga tggttatatt tggcgtacag ccgatgatgg
     5821 cgatgtccga gattcccacg atcacatgaa aggtaaattc gtccgctggg attcacctcc
     5881 aactttggac ggcatgaccg gccacgcggg cgagctgcca aactgccgct gctataaaga
     5941 gatcgtgttt gttcgcgttc cattcgcaat gaaaagggca gcataaccca tgaaatactt
     6001 ttttgagacc aggctcgggg aaacccgata ccgcctggct gacggctcgt tgctgtgcaa
     6061 agacgtgccg ataggacgaa caggtaagca gctctatggt gctgatgacc tgccaaaact
     6121 gaaacccgat aagttcggtg aaatagtcgt cacgcgttct cctgagcagg tattccatcc
     6181 ggccacgctt gcctcattcg aagggatgag catcacgatc ctgcatcctg aagatgaaaa
     6241 cgggaatgtg cggctggtaa atcccgagaa ctggaaagag cttgctgtcg ggcacctcca
     6301 gaatgtccgg cgcgggacgg gtgagcagtc tgatttgatg ctggctgacc ttatcgtcaa
     6361 agacgaaagc gccattcagc ttatcgaaga tggcctgcgc gaagtgtcgt gcggctatga
     6421 cgcggagtac gagcagaccg agccaggtaa agccgagcag gtcgatatta ccggaaacca
     6481 tgtggctctt gtccctaaag gcagagccgg aaatcgttgt gcaattggag acagagacac
     6541 aatggcaaat caaaagaaaa actggtggaa ccgcatgcgt gcggccatca agacaggaga
     6601 tgccgacacc atgaacgaac tggtggagtc ggctcccgca tcggttacag gagatgaggg
     6661 ggatttgccg cagggcgtta atctcaacat caacctgtcc ccgcagcaac cactaccgga
     6721 caaagcacca gagatgggcg gagatccaac cggcgacagt gatgatgacc tcaaaacatt
     6781 actgaaagcc ctgctggcta agctggaaag aaatgccacg ggcgataacg ataataagcc
     6841 tgacgataat ccgaccggtg acggcgagga cgatgaagag gaaaccacga ttactggtga
     6901 ctcagcctgg cgtgccgaag ttatcgttcc gggtatcgat ctgagccgta agatgaaacc
     6961 gaccgcgttc aaacgcgagg ttctggcttc tgctgacaaa acgctggttc gccagatagt
     7021 cggtgatgcg gatatccgca aattaccgaa acaatcggtc gacatggcgt ttaatgccgt
     7081 gtctgaggtt gccaaagggc gaaacacccg cgccaccacc ggcgatgcac agcgcctaaa
     7141 catgggcatg accagtatcg cttccctgaa caaacaaaac gctgaattct gggcaaaccg
     7201 taaagggtaa aaaatgaata atgtatttct gtaccggatg cctgttggca ttgccggggc
     7261 tgtctctcgc ccgcaggact taaccgtcga accggtggtc cttaaatccg ataacgcctt
     7321 cgctgcctat ggcctggctg gtaaatacga tgatgacggt tttttcgtgc cgctggcaga
     7381 tggtgatacc gcagacaagg tgaaggggat ctacgtgcgc ccttatccga ccacgtcgca
     7441 gccggacatg gttcgccagg tgggaacagg caagaacttc ccgggcgacg ccatgaagcg
     7501 tggctacgtg accgttaatc tcggttctga ttttgatgcc agcactatca aaaaaggcga
     7561 tccggtatac gttgtcgtct ccactgatga atccatcaaa gtgccgctgg gtggattcat
     7621 gtccacgtca gtcagtggca aaaacgtggt gctgaccaac gctgaattca caggtgccgg
     7681 tgatgctgac ggcaatgcag aaatttcctg gaagatttaa ggaacagacg aa
//
LOCUS       DAZSBF010000197         7715 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZSBF010000197 DAZSBF010000000
VERSION     DAZSBF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.200"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHXWD_003381"
     CDS             <1..643
                     /locus_tag="ACHXWD_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7622205.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHXWD_003382"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHXWD_003382"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR7622206.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHXWD_003383"
     CDS             2474..2986
                     /locus_tag="ACHXWD_003383"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR7622207.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHXWD_003384"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHXWD_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR7622208.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHXWD_003385"
     CDS             5579..5782
                     /locus_tag="ACHXWD_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR7622209.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHXWD_003386"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHXWD_003386"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR7622210.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="ACHXWD_003387"
     CDS             complement(6827..>7715)
                     /locus_tag="ACHXWD_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR7622211.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1785 a         2101 c         2020 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg gacgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAZSBF010000198         7691 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZSBF010000198 DAZSBF010000000
VERSION     DAZSBF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.201"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(229..933)
                     /locus_tag="ACHXWD_003388"
     CDS             complement(229..933)
                     /locus_tag="ACHXWD_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR7622212.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1070..1522)
                     /gene="moaE"
                     /locus_tag="ACHXWD_003389"
     CDS             complement(1070..1522)
                     /gene="moaE"
                     /locus_tag="ACHXWD_003389"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR7622213.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1524..1769)
                     /gene="moaD"
                     /locus_tag="ACHXWD_003390"
     CDS             complement(1524..1769)
                     /gene="moaD"
                     /locus_tag="ACHXWD_003390"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR7622214.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1762..2247)
                     /gene="moaC"
                     /locus_tag="ACHXWD_003391"
     CDS             complement(1762..2247)
                     /gene="moaC"
                     /locus_tag="ACHXWD_003391"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR7622215.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2250..2762)
                     /gene="moaB"
                     /locus_tag="ACHXWD_003392"
     CDS             complement(2250..2762)
                     /gene="moaB"
                     /locus_tag="ACHXWD_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR7622216.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2784..3773)
                     /gene="moaA"
                     /locus_tag="ACHXWD_003393"
     CDS             complement(2784..3773)
                     /gene="moaA"
                     /locus_tag="ACHXWD_003393"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR7622217.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3761..3905)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4170..5078
                     /gene="yvcK"
                     /locus_tag="ACHXWD_003394"
     CDS             4170..5078
                     /gene="yvcK"
                     /locus_tag="ACHXWD_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR7622218.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5270..7291)
                     /gene="uvrB"
                     /locus_tag="ACHXWD_003395"
     CDS             complement(5270..7291)
                     /gene="uvrB"
                     /locus_tag="ACHXWD_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR7622219.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1857 a         2035 c         1907 g         1892 t
ORIGIN      
        1 gctgttttgc gagtatgatt ccattctttt agttgtcctt agaaaaaata ttaatattca
       61 ccatcaataa atttaaaacc acgcatacaa aatacttttg tatttatgga tacggaaaac
      121 gattccgctc gctcataagg catattttat acctggtact tgttaagtgt cgtttttagt
      181 aagaaaaaaa tcccgacaca tggccgggat agtgatggtg aaaaagaatt aacggcggtt
      241 gccgaagatc cgtaacaaca tcaggaacag gttgatgaag tccagataca aggttaacgc
      301 gccaagaatg gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct gctcacccat
      361 atttttcaat ttctgagtgt catacgccgt caatccgaca aagacaatca cgccgatgta
      421 ggtaactgcc cacatcaatg cttcgctttt cagccagaag ttgaccagcg atgccagcac
      481 aatgccgatt aacgccataa acagcatatt gccaaagcca cttaaatcgc gcttcgtggt
      541 gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag tactggcgat
      601 agaagcagcg gtatagacaa tgaatatact ggaaagcgta agacccgtca gcgccgaata
      661 aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg ctgataacac
      721 aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt tagtgaacaa
      781 cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgcgg tcagcaacaa
      841 gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag cccggggttg
      901 tacgattgaa tcagaacgtg ggaatctgtc catgacgatc tcctgaagat ataaggaata
      961 tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac gcatgtactg
     1021 agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaaaaaac taccagcgtt
     1081 ttgccgcctg ctgatcgctc tcccgggctt caacccagcg gtcgccttcc ggcgtggctt
     1141 cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa
     1201 acgcactgct gcgatgcgca ctggtgacac cgacaaaaac gatttcatcg cccggccata
     1261 attccccgat gcggtgaatc accgtgacgc gccccagcgg ccagcggtta cgcgcttcat
     1321 caacgatttc tgccagtgct ttttcagtca tccccggata gtgttcgagg gttaatgctt
     1381 tgacgctgtc acccaggtta tggttgcgca ccttaccggt aaacgtgacc actgcaccgt
     1441 cttcgtcacg ctcagccagc cacgggtact cttctcctac gctgaacggc tgcgggccga
     1501 caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agctacttcg
     1561 tcgccgtcag tcagcggatg gtcaaaactc accagcgtct ggttgacggc agccagcaat
     1621 ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccaggtg ctggcgtaac
     1681 gcttcaacgg ttgggtaatc cgcagccact tcggttgcat ctgttcccac caactcgcgc
     1741 acctgggcga aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac ccgacttgcc
     1801 gccgctcttc gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca
     1861 catgtcataa atggtcagcg ccgccacgga ggccgcggtt aacgcttcca tttcgacacc
     1921 ggttttcccg gtcaggcggc ataaggtttc tatacgcacc cgattgtgct ccggctcggc
     1981 ctgtaaattg acttcaactt tgctgagcat cagcgggtga cacagcggga tcagatccca
     2041 ggtgcgtttt gccgcctgaa taccgacaat acgcgcagtg gcaaatacgt cgcctttgtg
     2101 gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttccgc
     2161 ccgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc
     2221 ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac
     2281 ggacgcgtac gggcatccag ctgcggcgcg atgatatttt cccatgcggt acggcacgct
     2341 ttggtcgaac ccggcatggc gaaaatcagc gttttgttgg cgacgcccgc taccgcacga
     2401 gattgcaacg tggaagtgcc aatctcttca aacgacaaca tccggaacac ttcaccaaaa
     2461 ccttcaactt cacggtcgaa caacggcagc aatgcttcgg gagcctgatc accttccgtc
     2521 aggccagtcc cccccgtaat caacaccact tgtacatcgt cgctggcgat ccacgcagat
     2581 acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg
     2641 cccgcttctt gcgccgaatc gcgcaaatag tgaccggagg tatcgtcttc ttcaccgcga
     2701 cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc
     2761 atctgatctc tccttttgac gttttagccg ccaatgtacg ataagttttg cgtaataccg
     2821 gtgttgtttt gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc tgaaatacgc
     2881 gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct
     2941 tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg gttgcaagtg
     3001 gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta atctggatgg
     3061 caaaagactt gcgcaggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc
     3121 agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcactgccc
     3181 tcgcccgttt ccatcagttc gatgaaacgc agctggatag ggcgatgctg gatccagttc
     3241 agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt gactttgacc
     3301 ttctcaaaac cgacctcaaa tgcagcatcg atccctgcca tgacctggtt gaatttatcc
     3361 tgcccggtaa tagcgtgaaa ctggcgggcg tccagactgt cgacactgac gttaatgcca
     3421 gtaagtcccg catcgcgcca gttcgccaca tcgcgttcca gacggtaacc attggtggtg
     3481 accgcaatct ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc ggtaaagtcg
     3541 cggcgtaaag acggctctcc tcctgtcagg cgcacttttt cggtgcccag actggcgaag
     3601 gcgcgcgtaa cccggcgaat ttcatcgacg gtaagaaagc ctttattggt gacgccgctc
     3661 ggtttgtagc catccggcag gcagtaggtg caacgaaagt tacacacatc ggtaatcgac
     3721 aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc
     3781 tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacgagc
     3841 attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga gtgtttagtg
     3901 gttaagccga tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc
     3961 atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat ggcagtttta
     4021 ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagaggt agaagaaaat
     4081 agtttgtatc ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg cgctactgta
     4141 gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct gatcttgatc
     4201 gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt
     4261 tgggttctcg tttaacgggt atcgtcacca ccacggataa tggtggctcg acggggcgta
     4321 ttcgccgttc agaaggcggc attgcctggg gcgatatgcg caactgcctc aaccaactga
     4381 taacggaacc gagcgtcgcc tccgcgatgt ttgaataccg ttttggcggc aatggcgaac
     4441 tttccggtca taatctcgga aacttgatgt taaaggcgct ggatcacctt agcgtgcggc
     4501 ctctggaagc catcaattta attcgtaatc tgctgaaagt ggatacgcat ttgattccaa
     4561 tgtcagagca tcccgtcgat ctgatggcga ttgacgatca ggggcatgaa gtttacggcg
     4621 aggtcaatat cgaccagtta actacgccga ttcaagagtt attattaacg ccgaatgtac
     4681 ccgcaacgcg tgaggcggtt cacgctatca atgaagcgga tctcatcatt attgggcctg
     4741 gcagttttta taccagcctg atgccaattc tgctgctgaa ggaaatcgcc caggcattac
     4801 gccgcacgcc agcgccgatg gtctatatcg gcaatctggg gcgtgagttg agtttacctg
     4861 cggctaattt gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg
     4921 tcattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa gagcggattg
     4981 tgattcagga ggtactggag gccagcgata ttccgtatcg tcatgaccgc cagttgttac
     5041 ataacgcgct ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa gaaaaattcc
     5101 ggcttcctat tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg aacgccttat
     5161 ccggcctaca aaccgcgcta attcaatgta ttgcagagat catgtaggcc tgataagcgt
     5221 agcacatcag gcaattttgc ttttgtcatc tgtcttcttc gttatcctgt cacgaagccg
     5281 cgataaacag ctcacgcagc tgatgcaact ggtcacgaat ttgtgccgct tcttcgaact
     5341 ccagattctg cgcgtgttgc atcattaacc cttccagctc atggattttc tgttgcaacg
     5401 ctttaggcga catatccatc ggcacattat ccggctcaac aatcgggcgc gattttcctc
     5461 tgcccttcgc tttggttttg gcaatgttct gccccagcgc caggatatcg accactttct
     5521 tgttcaagcc ttgcggcgta attccgtgtt cctcgttgta cttctgctgt ttctcacggc
     5581 gacgttcggt ttcgccaatc gctttcgcca ttgatggggt gatcttatcg ccgtagagaa
     5641 tcgctttacc gttaacgtta cgtgccgcac gaccaatagt ctggatcaac gaacgttcgg
     5701 aacgcaggaa gccttctttg tcagcgtcga ggatcgccac cagcgacact tccggcatat
     5761 ccagaccttc gcgcagtaag ttgatcccta ccagcacgtc gaactcaccc agacgcaagt
     5821 cgcggataat ctccatacgt tcgacggtgt cgatatctga atgaagataa cgcacgcgct
     5881 cgccatgttc ttcgagatat tcggtaagat cttccgccat ccgcttggtc agtgtggtga
     5941 ccagtacgcg ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga tcatcaacct
     6001 gtgtcgccac cggccgcact tcgataatcg ggtcaagcaa tccggttgga cgcaccacct
     6061 gatccaccac atcgccgccg gatttttcca gctcgtaatt acccggcgtc gccgaaacat
     6121 agatggtttg cggcgctaat gcttcgaact cttcaaactt aagcggacgg ttatccagcg
     6181 ctgatggcag gcggaagccg tactccacca gtgtctcttt acgcgcccgg tcaccgcgat
     6241 acatgccgcc aatttgtgga atggtgacgt gagattcatc gaccaccagc agcccatcgg
     6301 caggcaggta atcaaacagc gtcggcggtg gctcacccgg tccacgaccg gagaggaagc
     6361 gcgagtagtt ttcaatcccc gaacagtagc ccagctcgtt catcatctcc agatcaaact
     6421 gggtacgctg ggtcagccgc tgctcttcca gcagtttgtt gttttccaac agcactttgc
     6481 gtctggcggc cagttcttct ttgatctcct ccatcgcctg tacgatgcgc tcgcgcggtg
     6541 tgacgtagtg cgttttcggg tagatggtaa aacgtggaat agtggaaaca atctgcccgg
     6601 tcagcgggtc aaataacgac aatcgttcca cttcctcgtc aaacagttcc acgcgaagtg
     6661 caatgtcatc cgattctgcc gggaagatat ctatcacctc gccacgaacg cggaaagtac
     6721 cacgctggaa tgcttgatca ttacgagcgt attgcagctc cgccagtcgg cgcagaatcg
     6781 cgcgctgatc gataatcata ccgaccgtga gatggagcat catcttgaga tataaatcag
     6841 gatcgcccag accataaatc gcggaaacag aggccactac aaccacatca cgccgctcca
     6901 gcatcgcttt ggtggcggat aaacgcatct gctcaatatg ttcgttaacc gaggcatctt
     6961 tctcaatgaa agtgtcggaa ctcggtacat aggcttccgg ctgatagtag tcgtagtagg
     7021 agacgaaata ctccaccgcg ttttccggga agaactcttt catttcgcca tacagctggg
     7081 ccgccagcgt tttgttgggc gcaagtacca tggttgggcg ctgaaggtca gcaatgacat
     7141 tggcaatggt gaaggttttc cctgagccag tcacgccaag taacgtctgg tgcgccaggc
     7201 catcttccag cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca gaaggtttaa
     7261 aagcggaatt cagtttgaac ggtttactca tgagtcgcta cctgaaggag ttgggcgggc
     7321 aggtatgtaa ttttactcgt cgtacttaat tatgccaaca aattatactg gataaaaaaa
     7381 cagttcatca ccataatatt tctgatacag cgtaaactcc gctgtcaatc atgagcaaaa
     7441 tttactctgt ggcgagataa aactccggcc ttaccgggtt atccccaaag caacggcttt
     7501 tttaacattt gtcaagatga gtgatgacag ttttatggca agagatgcct gttcagtgac
     7561 tgccattgca tttatctaac cagttaaaaa ataaaagata tttctttgag ccgtctttaa
     7621 cgccaattcg cgtgcaagcc gcgtattctc tcgcttgcct cgtgttttct aactctaata
     7681 cacatggtta t
//
LOCUS       DAZSBF010000199         7627 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZSBF010000199 DAZSBF010000000
VERSION     DAZSBF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.202"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHXWD_003396"
     CDS             complement(152..487)
                     /locus_tag="ACHXWD_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR7622220.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHXWD_003397"
     CDS             complement(491..835)
                     /locus_tag="ACHXWD_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR7622221.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHXWD_003398"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHXWD_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR7622222.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHXWD_003399"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHXWD_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR7622223.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHXWD_003400"
     CDS             1257..1376
                     /locus_tag="ACHXWD_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR7622224.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHXWD_003401"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHXWD_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR7622225.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHXWD_003402"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHXWD_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR7622226.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHXWD_003403"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHXWD_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR7622227.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHXWD_003404"
     CDS             4176..4544
                     /locus_tag="ACHXWD_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR7622228.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHXWD_003405"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHXWD_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR7622229.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHXWD_003406"
     CDS             5295..5588
                     /locus_tag="ACHXWD_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR7622230.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHXWD_003407"
     CDS             5660..6319
                     /locus_tag="ACHXWD_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR7622231.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHXWD_003408"
     CDS             6411..6857
                     /locus_tag="ACHXWD_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR7622232.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7627)
                     /gene="hlyE"
                     /locus_tag="ACHXWD_003409"
     CDS             complement(7064..>7627)
                     /gene="hlyE"
                     /locus_tag="ACHXWD_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR7622233.1"
                     /translation="IKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1959 a         1714 c         1772 g         2182 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcaccct ggttagaggc
     2281 gcgtaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ctccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaat
//
LOCUS       DAZSBF010000200         7602 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZSBF010000200 DAZSBF010000000
VERSION     DAZSBF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.203"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            91..1380
                     /gene="hpaG"
                     /locus_tag="ACHXWD_003410"
     CDS             91..1380
                     /gene="hpaG"
                     /locus_tag="ACHXWD_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR7622234.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1377..2843
                     /gene="hpaE"
                     /locus_tag="ACHXWD_003411"
     CDS             1377..2843
                     /gene="hpaE"
                     /locus_tag="ACHXWD_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR7622235.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2845..3696
                     /gene="hpaD"
                     /locus_tag="ACHXWD_003412"
     CDS             2845..3696
                     /gene="hpaD"
                     /locus_tag="ACHXWD_003412"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR7622236.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3706..4086
                     /locus_tag="ACHXWD_003413"
     CDS             3706..4086
                     /locus_tag="ACHXWD_003413"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR7622237.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4197..5000
                     /gene="hpaH"
                     /locus_tag="ACHXWD_003414"
     CDS             4197..5000
                     /gene="hpaH"
                     /locus_tag="ACHXWD_003414"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR7622238.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            5011..5799
                     /gene="hpaI"
                     /locus_tag="ACHXWD_003415"
     CDS             5011..5799
                     /gene="hpaI"
                     /locus_tag="ACHXWD_003415"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR7622239.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5974..7350
                     /gene="hpaX"
                     /locus_tag="ACHXWD_003416"
     CDS             5974..7350
                     /gene="hpaX"
                     /locus_tag="ACHXWD_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR7622240.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            7360..>7602
                     /locus_tag="ACHXWD_003417"
     CDS             7360..>7602
                     /locus_tag="ACHXWD_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR7622241.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         1747 a         2133 c         2152 g         1570 t
ORIGIN      
        1 catcacttac ttttgccaaa ctacgcaatc gggaataaaa gcaatcatta acatattaat
       61 gattaagatg atcgaatccg aggagtggta atgaaaggca ctatcttcgc cgtagcgttg
      121 aaccatcgca gccaacttga tgcatggcag gaagcgttcc agcaatcccc ctacaaagcc
      181 ccgcctaaaa ctgcggtctg gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa
      241 ccgattccct ttccacaggg tgaaaaggta ctgagcggtg cgactgttgc gctgattgtg
      301 ggaaaaacgg cgacgaaagt acgtgaagaa gatgcggcag agtacatcgc cggatatgcg
      361 ctggctaacg acgtcagcct gccggaagag agcttttacc gcccggcaat caaagcaaaa
      421 tgtcgtgatg gattctgccc cattggcgaa accgtggctc tcagcaatgt cgataatctg
      481 accatctata ccgagatcaa cgggcgtcct gccgatcact ggaataccgc cgatttacaa
      541 cgtaacgccg cgcagttgct gagcgccctg agcgaatttg ccacgctgaa tccaggcgac
      601 gccattctgc tcggcacgcc acaggcgcgc gtggaaatac agccaggcga tcgcgttcgt
      661 gttctcgcag aaggtttccc gccgctggaa aatccggtag tggacgaacg tgaagtgacc
      721 acgcacaaga gcttcccaac gcagccacac ccgcacggca cgctgtttgc cctcggcctg
      781 aactacgccg accacgccag cgaactggaa tttaagccac cggaagaacc gctggtgttc
      841 ctgaaagcgc caaataccct cactggcgat aaccagacct ccgtgcgtcc aaacaatatt
      901 gaatacatgc actatgaagc ggagctggtg gtagttattg gcaaacaggc gcgtaacgtc
      961 agcgaagccg atgccatgga ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt
     1021 cgtgactatc tggaaaacta ctaccgccct aacctgcggg taaaaagccg cgacggactg
     1081 acgccgatgc tttcaaccat cgtgccgaaa gaggcgatcc cggacccgca taatctgacc
     1141 cttcgcacct tcgtcaacgg cgagttacgc cagcaaggca ccaccgccga tctgatcttc
     1201 agcgtgccct tcctgatcgc ctacttaagt gaatttatga ccctgaatcc gggcgacatg
     1261 atcgccaccg gcacacccaa aggcttatct gacgtggtgc ctggcgatga agtagtggtg
     1321 gaagtagaag gcgtgggccg cctggtgaac cgaattgtga gtgaggatac agcgaaatga
     1381 aaaaagtaaa tcattggatc aacggcaaaa atgttgcagg taacgactac ttccagacca
     1441 ccaatccggc aacgggtgaa gtgctggcgg atgtggcctc tggcggtgaa gcggagatca
     1501 atcaggcggt agcggcagcg aaagaggcgt tcccgaaatg ggccaatctg ccgatgaaag
     1561 agcgtgcgcg cctgatgcgc cgtctgggcg atctgatcga ccagaacgtg ccagagatcg
     1621 ccgcgatgga aaccgcggac accggcctgc cgatccatca gaccaaaaat gtgttgatcc
     1681 cacgcgcttc ccacaacttt gaatttttcg cggaagtctg ccagcagatg aacggcaaga
     1741 cctatccggt tgacgacaag atgctcaact acacgctggt gcagccggtg ggcgtttgtg
     1801 cgctggtatc gccgtggaac gtaccgttta tgaccgccac atggaaggtc gcgccgtgtc
     1861 tggcgctggg caataccgcg gtactgaaaa tgtcggaact ctccccgctg accgctgaca
     1921 gcctgggtga gctggcgctg gaagccgata ttccggcagg cgtgctgaac gtggtacagg
     1981 gctacggcgc aaccgcaggg gatgcgctgg ttcgtcatca tgacgtacgt gccgtgtcgt
     2041 tcaccggcgg tacggccacc gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact
     2101 ccatggaact gggcggtaaa tcgccggtgc tgatttttga agatgccgat attgaacgcg
     2161 cgctggacgc cgccctgttc accatcttct cgatcaacgg cgaacgctgc accgccggtt
     2221 cgcgcatctt tattcagcaa agcatctacc cggaattcgt taaacgcttt gccgaacgcg
     2281 ccaaccgtct gcgcgtgggc gatccgaacg atccgaatac ccaggttggc gcgcttatta
     2341 gccagcagca ctgggaaaaa gtctccggct atatccgtct cggcattgaa gaaggcgcaa
     2401 ccctgctggc gggcggcccg gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca
     2461 acttcctgcg cccaaccgtg ctggcagacg ttgataaccg tatgcgagtc gcccaggaag
     2521 agattttcgg gccggtcgcc tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgtc
     2581 tggcaaacga cgtggagtac ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag
     2641 tgttacgcct ggcgcgtggc attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc
     2701 gtgacctgcg ccagccattt ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg
     2761 agtacagctt cgaagtgttc gcggaaatga agaacgtctg catttccatg ggcgaccatc
     2821 caattccgaa atggggagtc tgatatgggt aagttagcgt tagccgccaa gatcacgcac
     2881 gtaccgtcga tgtatcactc tgaactgcca gggaaaaacc acggttgccg ccagggcgcg
     2941 atcgacgggc ataaagagat cagcaagcgt tgccgggaaa tgggcgtcga taccattatc
     3001 gttttcgata cccactggct ggtcaacagt gcttatcaca tcaactgtgc agaccatttt
     3061 gaaggcgtct acaccagcaa cgagctgccg cattttatcc gcgatatgac ctacaactac
     3121 gagggcaacc cggagttggg gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg
     3181 gcaaaagcgc acaacattcc cagcctgaaa ctggaatacg gcacgctggt gccgatgcgc
     3241 tacatgaatg aagacaagca cttcaaagtg gtctccattt cggctttctg cacggttcac
     3301 gattttgccg acagccgcaa gctgggcgaa gcgattctga aagcgatcga acagtacgac
     3361 ggcaccgtgg cggtccttgc cagcggttcg ttatcgcacc gctttattga cgatcagcgt
     3421 gcggaagaag ggatgaacag ctacacccgc gagttcgacc gccagatgga cgagcgcgtg
     3481 gtgaagttgt ggcgcgaagg ccagttcaaa gagttctgca atatgctgcc ggagtacgcc
     3541 gactactgct acggcgaagg caatatgcac gacacggtga tgctgctggg gatgctcggc
     3601 tgggataaat acgacggcaa ggtggagttt atcaccgagc tattcccaag ctctggcacc
     3661 ggtcaggtta acgctgtttt cccgcttccc gcgtaaggac gttttatgcc gcactttatc
     3721 gttaaatgca gtgacaacat ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg
     3781 aatccgacgc tggcagccac gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat
     3841 tgggtcgata cctggcagat ggccgacggg cagcgtgatt atgccttcgt gcatatgacg
     3901 ttgaaaatcg gcgcaggtcg cagcctggaa agccgccagc aggcgggtga aatgctgttt
     3961 gaactgatta aaacgcactt cgccgccctg atggagagcc gcctgctggc gttgtcgttt
     4021 gagattgaag agctgcatcc gacgctgaat tttaaacaaa acaacgtgca cgcattgttt
     4081 aagtagcgcg cagattgccc ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg
     4141 tacaccgtag gccggataag gcgcagccgc atccggcaat gccacaggat atcgctatgt
     4201 tcgataaaca cacccacacc ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg
     4261 aacagatccg cgcgatctcg ctggattacc cggagatcac catcgaagac gcttacgcgg
     4321 tgcagcgtga atgggttcgc ctgaaaatcg ccgaaggtcg cacgctgaaa ggccacaaaa
     4381 tcggcctgac ctcgaaagcg atgcaggcaa gctcgcagat cagcgaaccg gattacggcg
     4441 cgctgctgga tgacatgttc ttccacgatg gcagcgatat cccgaccgat cgctttatcg
     4501 tgccgcgcat tgaagtggag ctggctttcg tgctggcaaa accgctgcgt gggccaaact
     4561 gcacgctgtt cgacgtctac aacgccacgg actacgtgat cccggcgctg gagctgatcg
     4621 acgctcgctg ccacaacatc gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca
     4681 tttctgataa cgccgccaac gccggggtga tcctcggcgg tcgtccgatc aaaccagacg
     4741 aactggatct gcgttggatc tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg
     4801 gcgtcgccgc tggcgtgctg aatcatccgg caaacggcgt ggcctggctg gcgaacaaac
     4861 tcgcaccgta tgacgtacaa ctggaagccg ggcaaatcat tctcggtggt tcgttcaccc
     4921 gcccggttcc ggcgcgtaag ggcgacacct tccacgtcga ttacggcaac atgggttcca
     4981 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gctttaaagc ggcgctgaaa
     5041 gcaggccgtc cgcagatcgg attatggctg gggctgagta gcagctacag cgcagagtta
     5101 ctggccggag caggattcga ctggttgttg atcgacggtg agcacacgcc gaataacgtg
     5161 caaaccgtgc tcacccagct acaggcgatt gcgccctacc ccagccagcc ggtagtacgt
     5221 ccgtcgtgga acgatccggt gcaaatcaaa caactgctgg acgtcggcac acaaaccttg
     5281 ctggtgccga tggtacaaaa cgctgacgaa gcccgtgaag cggtacgcgc cacccgttat
     5341 ccccccgccg gtattcgcgg tgtgggcagt gcgctggccc gcgcctcgcg ctggaatcgt
     5401 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
     5461 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggcgtcga cggcgtgttt
     5521 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
     5581 gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg
     5641 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
     5701 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctggc agcacggttt
     5761 ggtgcgcagg ccaccgccgt gaagcccggc gtgtattaat gccggatgcg ctgcgcttat
     5821 ccggcctaca ctcgcaccaa accgtaggcc ggataaggcg tttacgccgc atccggcaaa
     5881 aagccgtacc aaatcgcgga ccggataagg cgttacgccg catccgccat aaaccttgtc
     5941 gtaccctaca aaaatcccat tagaggaaga aaaatgagcg acacctcacc tgccataccg
     6001 gagagtatcg atccggcgaa tcagcataaa gcgctgactg ccggacaaca ggcggttatt
     6061 aagaagctat ttcgccgcct gagcgtcttt ctgttcgtgc tgtttatctt ctcgttcctt
     6121 gatcgcatca acatcggctt tgccggactc acgatgggac gcgacctcgg tctgagcgcc
     6181 accatgtttg gcctcgctac caccctgttc tatgccgctt atgtcatctt cggcattccc
     6241 agcaacatta tgctgagtat tgtcggtgcg cggcgctgga tcgccaccat catggtgctc
     6301 tggggcatcg cctctactgc caccatgttt gccactggcc ccaccagctt gtacgtactg
     6361 cgtatactgg ttggcattac cgaagccggc tttctgcctg gtattctgct gtatttaacc
     6421 ttctggtttc cggcctattt ccgcgcccgt gccaacgcct tgtttatggt agcaatgccg
     6481 gtaacgacag cgttgggctc gatcgtttcc ggctacattt tgtcgctgga tggcgtcatg
     6541 gcattaaaag gctggcagtg gctgtttttg ctggaaggct tcccgtcggt attactcggc
     6601 gtcatggtgt ggttctggct tgatgactca ccggacaaag ctaagtggct gacgaaagaa
     6661 gacaaaaaat gcctgcaaga gatgatggat aacgatcgtc tgacgctggt tcagccagag
     6721 ggagccatca gccaccacgc catgcaacaa cgcagtatgt ggcgggagat cttcactccg
     6781 gtggtgatga tgtataccct ggcgtatttc tgcctgacca acacacttag tgcgatcagc
     6841 atctggacac cgcagatcct gcaaagtttt aatcagggca gcagtaatat caccatcggc
     6901 atgctggccg ccgtaccgca gatttgtacc attctcggga tggtctactg gagccgtcac
     6961 tcagatcgcc gccaggaacg aaggcatcac accgcccttc cttatttgtt cgctgccgca
     7021 ggttggttac tggcttcggc aactgatcac aacatgatcc agatgctggg gatcattatg
     7081 gcttcgaccg gatcattcag cgcaatggcg attttctgga caacaccgga tcagtccatc
     7141 agcctgcggg cacgagcgat cggtattgcg gtgatcaacg ccactggcaa cattggttca
     7201 gcgttaagtc cgtttatgat cggctggttg aaagatttga ccggcagctt taacagtgga
     7261 ttgtggtttg ttgccgcgct gctggtgatt ggtgcgggga ttatctgggc gattccaatg
     7321 cagtcctccc gtccgcgagc gaccctgtaa ggaacgacga tgtgtgaccg tcagattgcc
     7381 aatattgata tcagcaaaga gtacgatgaa agcctgggca cggacgatgt gcattatcag
     7441 tcgttcgccc gcatggcggc attttttggc cgccatatgc tgccacatcg ccacgaacag
     7501 tactttcaga tgcatttcct caatagcgga cagattgagc tacagcttga cgatcatcgc
     7561 tactcggtgg aagcgcccct gtttgtcctg acgccgccgt ca
//
LOCUS       DAZSBF010000201         7579 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZSBF010000201 DAZSBF010000000
VERSION     DAZSBF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.204"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1217
                     /locus_tag="ACHXWD_003418"
     CDS             <1..1217
                     /locus_tag="ACHXWD_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR7622242.1"
                     /translation="LFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFIIIMAIYII
                     FVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQAVNTHS
                     EKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFLFQVFL
                     PLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNKSFNH
                     KLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNVLYV
                     PQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQKQK
                     LGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNIP
                     KAQIIDITKHEV"
     gene            1220..2113
                     /locus_tag="ACHXWD_003419"
     CDS             1220..2113
                     /locus_tag="ACHXWD_003419"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFR7622243.1"
                     /translation="MNNLIKKEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLE
                     KDILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVD
                     RCQSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKW
                     KVYNPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHI
                     SFTVIRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFA
                     ENMLKAQSRTYRYKKIKLEHI"
     gene            complement(2138..2500)
                     /locus_tag="ACHXWD_003420"
     CDS             complement(2138..2500)
                     /locus_tag="ACHXWD_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HFR7622244.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVI
                     YKKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNILMAFIEN
                     WIKVNGGWKMQFISYEKI"
     gene            complement(2497..2736)
                     /locus_tag="ACHXWD_003421"
     CDS             complement(2497..2736)
                     /locus_tag="ACHXWD_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="HFR7622245.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILK
                     RVIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
     gene            complement(3447..3680)
                     /locus_tag="ACHXWD_003422"
     CDS             complement(3447..3680)
                     /locus_tag="ACHXWD_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR7622246.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(3687..4205)
                     /locus_tag="ACHXWD_003423"
     CDS             complement(3687..4205)
                     /locus_tag="ACHXWD_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR7622247.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTV
                     SGGTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVI
                     PYQGKDWVQLTE"
     gene            complement(4243..4893)
                     /locus_tag="ACHXWD_003424"
                     /pseudo
     CDS             complement(4243..4893)
                     /locus_tag="ACHXWD_003424"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4883..5206)
                     /locus_tag="ACHXWD_003425"
     CDS             complement(4883..5206)
                     /locus_tag="ACHXWD_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR7622248.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQG
                     ARDDC"
     gene            5271..5462
                     /locus_tag="ACHXWD_003426"
     CDS             5271..5462
                     /locus_tag="ACHXWD_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR7622249.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            6121..6279
                     /locus_tag="ACHXWD_003427"
     CDS             6121..6279
                     /locus_tag="ACHXWD_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622250.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEI
                     LRVLEVVA"
     gene            6827..7525
                     /locus_tag="ACHXWD_003428"
     CDS             6827..7525
                     /locus_tag="ACHXWD_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622251.1"
                     /translation="MLQHKMNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPE
                     EADDIFNETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLG
                     AYLHHYPNTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDY
                     AVRYSIKNINTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGC
                     SGKGCGAGCSCNSR"
BASE COUNT         2268 a         1799 c         1416 g         2096 t
ORIGIN      
        1 cattatttta tacaatatgt tctgatgtag taccattatt aatacaaatg attggaataa
       61 tcataacaat ttgtataagt gttaatacat taatagccat tgagtttata ataataatgg
      121 caatctatat aatatttgtc ataaaaatga cacaacgtag atttccaatg atgaaatcag
      181 ttgcacttag ctccaaattc gcatctggaa gaatgtttga tatgatgcac atgtatccta
      241 tggataaagc tttccatacc acggataaaa gtcgtaaacg agtaattcaa gctgtaaata
      301 cacattcaga aaaacaaaga aaagtaaata atgaattttt cctgtttggt atcagctccg
      361 cttttctttc agtcattttt agtagtctga ttatcctatc agcatactgg atgtttcttc
      421 atggtagagc aagccgtggt agtattatta tgcttgccac ttttttattc caagtttttc
      481 tcccattaaa tcgcattggt tatctattta gacaaataaa aatggcaaga acagaaattg
      541 atttatactg tgccgaaata aacgacataa aaaaaacaaa ttacacagac aagcaatatc
      601 ttcatgtaaa agacaatatt tgcaatattg aaatatacaa caaatcattc aaccataaat
      661 taatattaac aaaaggcgtt gttaccttta taaccggtga gaatggttcg ggaaaaacaa
      721 ctattgcaaa aattctctct ggcaatataa caacagaaga aaacacaata aaaatcaatg
      781 ggatacaaca agaaaaagca aattctcctt ttgttaatgt cttatacgtt cctcaagatc
      841 tagatctcat gcctggaact atagaggaaa atatattaca ttattccggc attaataatt
      901 taaatacaat taaaaagctg ctacacagat ttaagttcga caaaccacta gattatgaga
      961 tcaaaggtta cggacataat ttatctggtg gacaaaaaca aaaattagga gtatctctta
     1021 catctggaaa aaacgtagac ttgattattt tcgatgaacc gacaaaaggg tttgattcac
     1081 ttggtataaa aataatcagt gattatataa aagaggaaaa caaggaaaag catatcattg
     1141 ttatatctca tgatgaacaa cttataaata atatacctaa agcacagatt atagatatca
     1201 caaaacatga ggtttaaaaa tgaataatct tataaaaaag gaaatcatag aaaaatttaa
     1261 gaaatataat ttccagaaat atccgtttgt attcacggat gttaattata aaaacttaat
     1321 caactggaat gatcttaata aattgcttga aaaagatata ttgcactatc ctagagttag
     1381 aatggcaaat gacaactttc ccgaaattag ggggtataaa ggatttataa gatacacata
     1441 tagccaaaca ggcgatagaa caccacatat aaatcgccat cagctatata aatgcttacg
     1501 tgatggcgca acccttatag tagatcgatg ccagtcattc tttgaatctg tagatgaaag
     1561 cagactatgg ttatctaaag agttagaatg tacatgtagt gctaatctat atgctgcatt
     1621 tacagcaaca ccaagctttg ggcttcattt tgacaatcat gatgtaatag ctgttcaaat
     1681 tgagggaata aaaaaatgga aagtttacaa ccctacctat tcataccctc tcgaagatga
     1741 aagaagcttc gattatctac cacctaatac ctccccagat tatgagtttg atataacccc
     1801 aggtcaggcc atatatcttc ctgctggata ctggcacaat gttacgactc agagcaaaca
     1861 ctctcttcat atatctttta cagttataag acctcgtcga ttagaactat ttaaaacatt
     1921 atttgatgaa ttaaaaaaca atccatatat gcgggaacct atagagcatg gtgattcatt
     1981 atctgataaa gaaaaaataa aaactataat aactaatgct ataaatagtt ttgatattca
     2041 gtttgcagaa aacatgctta aagctcaaag cagaacttat cgctataaaa aaataaaact
     2101 tgaacatata taaccattaa tctaaggggg cttcccctta gattttttca tacgatataa
     2161 actgcatttt ccatccacca ttaaccttta tccaattctc aataaaagcc attaatatat
     2221 ttccatcatt ttgctttatt tcagctttcc ctgaaacaca agcaatgcct ttaaactcac
     2281 gcattacgac atcatattca tatctttcta tgctaggtaa aatcctcgct ggacgcatat
     2341 caatctttcg taactgatgt tttttataga taactttttg accatttgtt gaaataaacc
     2401 aatcagcttc caagtaatct agcatttcag tattacctga ataaaatgca ttataccatt
     2461 cttttcttat tagatttaag tctgatttaa aattagtcac actaccttcc tcatatctaa
     2521 ttcctcctct aatctatccc caacattcct tactgatttt atagcatcat caatagccac
     2581 attatatatg actgggatta ctcttttaag aatctcatca agtaattcct ctgattcaaa
     2641 ctgtccaagg catatatcat gttcatctct gattttttgt gaaataaatt cagctagtcc
     2701 tttatatatg tttttttcaa tatcagaaat tttcattctt tatgaagcct cacaaataaa
     2761 ttaataatat aacatttttc ctatcatagg aaaattacaa ctcaaccata ctgcaacctg
     2821 gaatttccca agcaagcata tttaaataag tgcaggatac cacctcaaat cattctctaa
     2881 ggaacaagat aatttaccat aatcccttat tgtacgcacc gctgaaacgc gttcggcgcg
     2941 atcacggcag cagacaggta aaaatggcaa caaaccaccc gaaaaactgc cgcgatcgca
     3001 cccggtaaat tttaaccaca tgcatagcta tgcagccatg tgaatcacgc aggaattgcc
     3061 agccagagac agctgaaacg gatttttttc atgctttcgg aagcgaagaa gacggggacc
     3121 ggagcgggaa aacagaagtt caggggaatg agcgcgatct ggcaatagag gcaaaacaca
     3181 gcaacaaaag acacaccaga atcgcgcccg tatgcgtttt aacgcgttcc tgtgttttca
     3241 gcaaggtctg actgaagcgc gttcccggtg cccccgaacc aaccattatc ccaaaaaatg
     3301 caccggaaac gcgctgacaa cattttagcc tgtctctgat taccatccca gcgaagggcc
     3361 atcctgtgtc cgttcctgta tttccagctg gcgctcacgc tccagggata actcatgttc
     3421 gcgttcctgt atcagccgtt catcccttat ctcctgttct cgctgtataa ctggctcaag
     3481 cgttctgtct gctcgctcaa gtgctgcacc tgctgactca actgcatgac ccgctcgttc
     3541 agcatcgcgt tgtcccgttg cgtaagcgaa aacattttct gcaattccac gaaggcgctc
     3601 tcccattcgt tcagccgctg catatagtcc tgttgcagtt gctctaaggc gttcagcaaa
     3661 tgtctttcca gctccgtcac tctgtgtcac tccgtcagtt gtacccagtc cttcccctga
     3721 tagggaatca ccgttgccga tttcccctga gggataacca gaaatttcct tctcccgtcc
     3781 tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct gcaatgtttc ttcctgtatc
     3841 ctgatttctt ccaggttcgc ctgtatcatc ccgccaagat accagagcgt cccgccactc
     3901 acggtaaaca ggaaaaggac catccccagt aacatcatgc ccgtattccc tgccagcttt
     3961 aacacgtcct tcctgtgctg catcatcgcc tctttcaccc cttcccggtg ttttttcagt
     4021 gattcctctg tcgaagctgt gaacagggct atagcgtctt tgattttcgt ctcgtttgat
     4081 gtcacagcct tgcttacaga tttttcgagc ttgctgaact cgtcgttcag cattgtctct
     4141 gtagattcgg ctctctcttt cagctttttc tcgaactccg cgcccgtctg taaaagattg
     4201 ctcataaaat gctcctttca gcctgatatt cttcccaccg ttcgggtctg caatgctgat
     4261 actgcttcgc atcaccctga ccacttcaag ccctgcctct gtgagcgcct gaatcacatc
     4321 ctgacgacct tttatctccc cgacatgata aagagcgtct atcccgcgtg tgacgctctc
     4381 tgcaagcgcc tgtttcgttt ccggcaggtt atcagggaga gtcagtatcc ggcggttctc
     4441 cggggcgttg gggtcatgca gcccgtaatg gtgattcacc agcgtctgcc aggcatcaat
     4501 tctcggcctg tctgcccggt cgtagtacgg ctggaggcgt tttccggtct gtagctccat
     4561 gttcggaatg acaaaattca gctcaagccg tcccttgtcc tggtgctcca cccacaggat
     4621 gctgtactga ttcttttcga gaccgggcat cagtacacgc tcaaagctcg ccatcacctt
     4681 ttcacgtcct cccggcggca gctccttctc cgcgaacgac agaacacctg acgtgtattt
     4741 cttcgcaaat ggcgtggcat cgatgagttc ccggacttct tccggattac cctgaagcac
     4801 cgttgcccct tcccggtttc gctcccttcc cagcaggtaa tcaaccggac cactgccacc
     4861 gccttttccc ctggcatgaa atttaacaat catcccgcgc gccctgttcc ctgacggcca
     4921 gacgcagccg acgcagctcc tccccgatgg ccatcagcgc agccaacacc tgaacccgat
     4981 caccggaaga ccactgcccg ctgttcacct tcctcgctgt ctgattcagg ttattcccga
     5041 tggcggccag ctgacgcagt aacggcggtg ccagtgtcgg cagcttcccg gagcgtgcaa
     5101 ccggctcacc caggcagacc cgccgcatcc ataccgccag ctgtttaccc tcacagcgtt
     5161 ccagtaaccg ggcatgttca tcatcagtaa cccgtatggt gagcatcctc tcgcgtttca
     5221 tcggtatcat taccccataa aacagaaatc ccccttacac ggaggcatca gtgactaaac
     5281 aggaaaaaac cgcccttaac atggcccgtt ttatcagaag ccagacatta acgctgctgg
     5341 agaaactcaa cgaactggac gcagatgaac aggccgatat ttgtgaatca cttcacgacc
     5401 acgccgacga gctttaccgc agctgcctcg cacgcttcgg ggatgacggt gaaaacctct
     5461 gacacataca gctcccggag acggtaacag cttgtctgtg agcggatgcc gggagcagac
     5521 aagcccctca gggcgcgtca gggggtttcg gggcgaagcc ctgaaccagt cacgtagcga
     5581 tagcggagtg tatactggct taactatgcg gcatcagtac gaattgtatg gaaagcgcac
     5641 catgtccgat gtgaaatgcc gcacagatgc gtaaggagaa aatgcacgtt ccggcgctct
     5701 tccgcttcct cgctcactga ctcgctacac tcggtcgttc ggctgcggcg agcggtgtct
     5761 gctcactcaa aagcggagat gcggttatcc acagaatcag gggataacgc caaaagaaac
     5821 atgtgagcaa aaaacgagaa ccaggaaaag gctgcgccgt tggcgttttt ccataggctc
     5881 cgcccccctg acgagcatca taaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5941 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6001 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6061 catagctcac gctgtaggta tctcggctcg gtgtaggtcg ttcgctccaa gctgggctgt
     6121 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6181 tccaacccgg taagacacga caaagcgcca ctggcagcag ccactggtaa ctggacaagg
     6241 tggattgaga tattaagagt tcttgaagtg gtggcctaac tgcggctaca ctagaaggac
     6301 agtatttggt atctgtgctc caccaagcca gttacccggt taagcagtcc ccaactgact
     6361 taaccttcga ctaaaccgcc tccccaggcg gttttttcgt ttactggcag cagattacgc
     6421 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttt gagatccgcc cactcctgga
     6481 acctacaaca catgtgtaaa acgtcaatgg agggggctat tatcggactg caacacattt
     6541 cgtacagcct ttacactcgg tgaattagcg gccctagatg catccactca ttcaaccaac
     6601 tcaattctct tctcttagag tgggaaaatt ttgttttcct tgcagcccct acccgtaaaa
     6661 actggctgca aaaaccttgt attactaatg gatcctataa ccattaaaaa acttgattac
     6721 tatctcattt atagaattag gcatattgac acaagcacaa atttaacaat accataacaa
     6781 atgataacta ttctcatcta cattcaaata tataattggg ggtgttatgc tacaacataa
     6841 gatgaacagc agttcttatg caaaagttca taatgttagc tcattagaag atatcatgag
     6901 ttatcacaat gatgatgttc ttctaaaatt tcgtaaagaa tggaatgtaa caccagaaga
     6961 agctgatgat atctttaatg aaacaaagaa attcatctgg ctagcctcaa catgcctaac
     7021 ggaatgctac aatataaaag ttcacgagca attacaaatt atagatgaaa tgtggcatac
     7081 gtttattcaa tttacagatg cttataccag cttttgtgaa aaatatcttg gtgcttacct
     7141 ccaccattat ccaaatacaa atgacatgct aaaaaatgag ataaggcatg ttaatgagca
     7201 tggtataaca ttccaagaat atcgttttaa cgaatataaa aatcaaattg aaaaaatcgc
     7261 tttttaccta ggtcatgaaa ctgtcgcaaa gtggtatggt gattatgctg taagatacag
     7321 cataaagaac attaatacta taagaattcc aaaagaatcc atatccagtg attcttacat
     7381 cgaaaaagta aagagtatca ctcaccttcc agccgcagaa tttgtaaaaa taataatgcg
     7441 aaaagatgtt tggaatgata atggttctgt ttgtggttgc agcggtaaag gatgtggcgc
     7501 tgggtgctca tgtaattcta gataacataa agcccgtaat atacgggctt taaggattat
     7561 aaattgaatt ttattgaaa
//
LOCUS       DAZSBF010000202         7491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZSBF010000202 DAZSBF010000000
VERSION     DAZSBF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.205"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHXWD_003429"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHXWD_003429"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR7622252.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVTYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHXWD_003430"
     tRNA            647..722
                     /locus_tag="ACHXWD_003430"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHXWD_003431"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHXWD_003431"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR7622253.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHXWD_003432"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHXWD_003432"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR7622254.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHXWD_003433"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHXWD_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR7622255.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHXWD_003434"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHXWD_003434"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR7622256.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHXWD_003435"
     CDS             complement(5917..6249)
                     /locus_tag="ACHXWD_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR7622257.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHXWD_003436"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHXWD_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR7622258.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1824 a         2029 c         1869 g         1769 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat gtcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaatacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacag tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgca
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZSBF010000203         7483 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZSBF010000203 DAZSBF010000000
VERSION     DAZSBF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.206"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="ACHXWD_003437"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="ACHXWD_003437"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR7622259.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2666)
                     /locus_tag="ACHXWD_003438"
     CDS             complement(1671..2666)
                     /locus_tag="ACHXWD_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7622260.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="ACHXWD_003439"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="ACHXWD_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHXWD_003440"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="ACHXWD_003440"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR7622261.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHXWD_003441"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="ACHXWD_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR7622262.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="ACHXWD_003442"
     CDS             complement(6107..7270)
                     /gene="emrK"
                     /locus_tag="ACHXWD_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR7622263.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2076 a         1763 c         1715 g         1929 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa aagtattttt ttctgttaga ctgtttttta tttgaattaa
     7261 tctgttccac tattatctct catttcgcat agatgaaatt tatgaattga atgaaaagag
     7321 tattcattct tcatatcaaa cacagtgcat gtgttgcaca ttatcccagg aacgttggag
     7381 gcctaattta aatgaggtgc aaaaaaaaca acagaggact aaaccgtggc ttttgcaata
     7441 caattcttac gcctgtagga ttagtgagaa gacttatagt gct
//
LOCUS       DAZSBF010000204         7470 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZSBF010000204 DAZSBF010000000
VERSION     DAZSBF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7470
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.207"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHXWD_003443"
     CDS             <1..108
                     /locus_tag="ACHXWD_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR7622264.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHXWD_003444"
     CDS             complement(149..1231)
                     /locus_tag="ACHXWD_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR7622265.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHXWD_003445"
     CDS             complement(1228..3060)
                     /locus_tag="ACHXWD_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR7622266.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHXWD_003446"
     CDS             complement(3275..3631)
                     /locus_tag="ACHXWD_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622267.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHXWD_003447"
     CDS             complement(3689..4111)
                     /locus_tag="ACHXWD_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7622268.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHXWD_003448"
     CDS             complement(4127..4852)
                     /locus_tag="ACHXWD_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR7622269.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHXWD_003449"
     CDS             complement(4874..5620)
                     /locus_tag="ACHXWD_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR7622270.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHXWD_003450"
     CDS             complement(5627..6691)
                     /locus_tag="ACHXWD_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR7622271.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGGPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHXWD_003451"
     CDS             complement(6763..7188)
                     /locus_tag="ACHXWD_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR7622272.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..>7470)
                     /locus_tag="ACHXWD_003452"
     CDS             complement(7185..>7470)
                     /locus_tag="ACHXWD_003452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622273.1"
                     /translation="THHIGRIMNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAK
                     LTGEKILRWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
BASE COUNT         1820 a         2046 c         1551 g         2053 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggcc ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt
//
LOCUS       DAZSBF010000205         7392 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZSBF010000205 DAZSBF010000000
VERSION     DAZSBF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.209"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(34..110)
                     /locus_tag="ACHXWD_003453"
     tRNA            complement(34..110)
                     /locus_tag="ACHXWD_003453"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(74..76),aa:Arg,seq:acg)
     gene            complement(114..206)
                     /locus_tag="ACHXWD_003454"
     tRNA            complement(114..206)
                     /locus_tag="ACHXWD_003454"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(170..172),aa:Ser,seq:gct)
     gene            complement(522..707)
                     /gene="csrA"
                     /locus_tag="ACHXWD_003455"
     CDS             complement(522..707)
                     /gene="csrA"
                     /locus_tag="ACHXWD_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR7622274.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(942..3572)
                     /gene="alaS"
                     /locus_tag="ACHXWD_003456"
     CDS             complement(942..3572)
                     /gene="alaS"
                     /locus_tag="ACHXWD_003456"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR7622275.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3700..4200)
                     /gene="recX"
                     /locus_tag="ACHXWD_003457"
     CDS             complement(3700..4200)
                     /gene="recX"
                     /locus_tag="ACHXWD_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR7622276.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            complement(4428..5489)
                     /gene="recA"
                     /locus_tag="ACHXWD_003458"
     CDS             complement(4428..5489)
                     /gene="recA"
                     /locus_tag="ACHXWD_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR7622277.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5569..6066)
                     /gene="pncC"
                     /locus_tag="ACHXWD_003459"
     CDS             complement(5569..6066)
                     /gene="pncC"
                     /locus_tag="ACHXWD_003459"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR7622278.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6211..7296)
                     /gene="mltB"
                     /locus_tag="ACHXWD_003460"
     CDS             complement(6211..7296)
                     /gene="mltB"
                     /locus_tag="ACHXWD_003460"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR7622279.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1729 a         2076 c         1788 g         1799 t
ORIGIN      
        1 gataccgcta ctaaagccat atcaagtaag agatggtgca tccgggagga ttcgaacctc
       61 cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc aaatggcggt
      121 gaggcgggga ttcgaacccc ggatgcagct tttgaccgca tactccctta gcaggggagc
      181 gccttcagcc tctcggccac ctcaccacac gcctcttacg agtgcttcga agaacttgtt
      241 tatcgctcat cgtcgctgcg tggcgcacat attactttct gagacttata agtcaaacaa
      301 tttttcccac acttttatcg tttgcacact tcacgctcaa ttagtctgta aaaacgtcaa
      361 aaagagtgtt ttatctacag aaaaacggag gtctggcaga tagcagtaat gcaaaaaaat
      421 ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac tatagagtga aggagaaatt
      481 ttgagggtgc gtctcaccga taaagatgag acgcggaaag attagtaact ggactgctgg
      541 gatttttcag cctggatacg ctggtagatc tcttcacggt gaacagaaac ttccttcggg
      601 gcatttacgc caatacgtac ctggttgccc tttaccccta aaactgtcac ggtgacctca
      661 tccccaatca tgagggtctc accaactcga cgagtcagaa tcagcattct ttgctccttg
      721 aaagattaaa agagtcgggt ctctctgtat cccggcatta tccatcatat aacgccaaaa
      781 agtaagcaat gacaaacaca ttacatgtaa gcagtcatgg cattacattc tgttaaacct
      841 aagtttagcc gatatacaca acttcaacct gactttatcg ttgtcgatag cgttaatgcg
      901 aatgccgtga agcgagtcca cggcattgcc tgacgcttat attattgcaa tttcgcgctg
      961 acccagcctt tcacactggc taacgctgca ggtaaggccg cagcatccgt accaccggct
     1021 tgcgccatgt caggacgtcc accacccttg ccgcccacct gctgagcgac cataccaatc
     1081 agttcccctg ctttcacacg atctgtgacg tccttagata cgcctgcaat cagagaaacc
     1141 ttaccttcgg ctaccgttgc cagcacgata attgtcgacc ccagctgatt ttttaaatcg
     1201 tcaaccatgg tacgcaacat tttcggctca acaccgctaa gctcgctaac caacagctta
     1261 acaccattaa catcaattgc cttactggaa agatttgcgc tctcctgtgc ggcagcttgt
     1321 tctttaagct gttgtagttc tttttctaac tgacgcgtac gttccagtac tgagcgcact
     1381 ttatccgcca gattattgct atcgcctttc agcagatgcg cgacttcgct taagcgatcg
     1441 ctgtctgcat gaacggtggt gatagcgcct tctccggtta ccgcttcgat acgacgaacg
     1501 cctgcagcag tacccgattc agagatgatg cggaacagac caatatcacc agtgcggctg
     1561 gcgtgagtac cgccacacag ctcggtggag aaatcgccca tgctcagcac gcgtacacgc
     1621 tcatcatact tctcgccgaa cagcgccatc gcacctttcg ctttcgccgc ttcgagatcc
     1681 atgatgttgg tttcgatcgg caaattgcga cgaatctgtg cgttcaccag gtcttcgacc
     1741 gcacgaatct cttccggttt catcgcttcg ttgtgtgaga agtcgaagcg cagcaccttg
     1801 tcgttaacca gtgaaccttt ctgcgataca tgagtcccca gaacctggcg cagcgcagcg
     1861 tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc ctcatcaaca
     1921 tcagcctgca cagcgtcgcc cactttcaga gaacccgcag caagtttacc gatgtgacca
     1981 atcgcctggc cgtatttctg cgtatcttcc accgcgaagg agaagttagc gcctttcagt
     2041 tcgcctttat caccaacctg accgccggat tccgcataga atggcgtttg atccagcacg
     2101 accacagctt cctggcctgc attgatggca tcaaccgctt taccatcaac aaacagcgcg
     2161 gtcactttgc cgttcagttc cagatggtca tagcctttaa attcagatgc actatcaaca
     2221 cggatcattg cgttgtaatc ggcaccaaag ccgctggctt cgcgcgcgcg acgacgctgc
     2281 tcttccattg ctgcttcaaa accagcttcg tcaactttga tgttgcgctc acgacaaaca
     2341 tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca gacggaaagc cgtttcacca
     2401 tccagcgtat caccagaaag ttttgccagc tcttcatcca gcaacgccag accgcgctcc
     2461 agagtacggg caaactgctc ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg
     2521 cgtttcaggt cttcacccgc agagcccata acgtcgatca gcggaccaac cagtttgtag
     2581 aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca ctgcgcgacg aatgatacga
     2641 cgcagtacat aaccacggtt ttcattggac ggcattacgc catccgcgat caggaacgca
     2701 caagaacgaa tgtggtcagc gattacgcgc agcgatttat tgctcagatc ggtcgcgcca
     2761 gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt cgatgtcata gttagagtta
     2821 acgtgttgca gcaccgcagc aatacgctcc agacccatac cggtatctac agacggcttc
     2881 ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact gcatgaagac gatgttccag
     2941 atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg gagggccacc ccaaatgtgg
     3001 tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc acccatctgc
     3061 cagaagttgt cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg
     3121 cgcgggatcc ctacttcttt ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg
     3181 gtaacccaca gacgctcttt cggcagggca aaccattttt cgctggtcag cagttcccat
     3241 gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg
     3301 aagaaggtat ggtgacgcgc ggtgtaaccg acattttcca ggtcgttgtg tttaccaccc
     3361 gcacgcacgc agcgttggga agtggtagcg cgggaataat tacgcttgtc gagtccaagg
     3421 aacacatcct tgaactggtt catcccggcg ttggtaaaca acaaagttgg gtcgttatgg
     3481 ggtaccaggg agctgctggc aactacctga tgtcccttac tatggaaaaa gtcgagaaac
     3541 gcctgacgga tctcagcggt gctcttgctc ataattatcc tgaaatcaag ctaacgaaat
     3601 atcgccacca gctccagcgt gtcttaaccg ccgggctggt aactgaaaag tgggaataag
     3661 ataagttttc ttgagtggga agtaaaatac cgtatgcgtt cagtcggcaa aatttagcca
     3721 aatatcctgg atatcttcca tcagatagcc acgatagagc agaaaacgct ggatcttaac
     3781 tttttctgaa aagacagttg gcaaaggttc accatatttt cgcgtcgcct gatcgcgcac
     3841 cagtgcgcac cagtcgatgt cacattcacg catcgctttt tctgtcgctt cgcgggaaat
     3901 acctttctga ttcagttcct gacgaatacg tgcaggtcca taacctttgc ggctacggct
     3961 ggcgataaag cgcgcaacaa atcggctgtc atcgagatag ccatgttcat ggcaccaggc
     4021 aataacgcgc tcgtaatctt ctgccgtagc atcaatctct tctgggccat ttttgcccat
     4081 aatcggtgcc gcgagtttac gtcgcagttc ttgctcactg tgatcgcgca ccgccagaat
     4141 gcgtaccgca cgatccaaca ggcgagcata tgccgggcga cgggatgttg attctgtcat
     4201 ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga tgcgacccag gttttgctga
     4261 taaacagatt taatagtcaa tgagtgtgca gccaattcgt tagaggatcg aggtgagagt
     4321 aatttttcac gccagtgttg gcctgttccc ctcaccctaa ccctctcccc aaaggggcga
     4381 ggggaccgtc cgtgcacaca ttatctatta tcatcaataa cgaacgatta aaaatcttcg
     4441 ttagtttctg ctacgccttc gctgtcatct acagagaaat ccggcgttga gttcgggttg
     4501 ctcagcagca actcacgtac tttcttctcg atctctttcg cggtttccgg gttatctttc
     4561 agccaggcag ttgcattcgc tttaccctga ccgatcttct cacctttgta gctgtaccac
     4621 gcgcctgctt tctcgatcag cttctctttt acgcccaggt caaccagttc gccgtagaag
     4681 ttgatacctt cgccgtagag gatctgaaat tcagcctgtt taaacggtgc agcgattttg
     4741 ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt tttcgccctc tttcaccgcg
     4801 ccgatacgac ggatgtcgag acgaacagag gcgtagaatt tcagcgcgtt accaccggta
     4861 gtggtttccg ggttaccgaa catcacacca attttcatac ggatctggtt gatgaagatc
     4921 agcagcgtgt tggactgctt caggttaccc gccagcttac gcatcgcctg gctcatcata
     4981 cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt cgatttccgc tttcggcgtc
     5041 agtgccgcca cggagtcaac gacgataacg tctactgcgc cagaacgcgc cagggcgtca
     5101 cagatttcca gtgcctgctc gccggtgtcc ggctgggagc acagcaggtt atcgatatcg
     5161 acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt cagcatcgat aaacgcacag
     5221 gttttacctt cacgctgcgc tgcggcgatc acctgcagcg tcagcgtggt tttaccggaa
     5281 gattccggtc cgtagatttc gacgatacgg cccatcggca gaccacctgc cccaagcgcg
     5341 atatccagtg aaagcgaacc ggtagagatg gtttccacat ccatggaacg gtcttcaccc
     5401 aggcgcatga tggagccttt accaaattgt ttctcaatct ggcccagtgc tgccgccaac
     5461 gctttctgtt tgttttcgtc gatagccatt tttactcctg tcatgccggg taataccgga
     5521 tagtcaatat gttctgttga agcaattata ctgtatactc atacagtatc aagtgttttg
     5581 tagaaattgt tgccacaagg tctgcaatgc atacgcagta gcctgacgac gcaccgcatc
     5641 acggtcgccg ctgaagcatt cccgccgggt aatgccttca ccgcgggcag tggcaaaagc
     5701 aaaccagacg gtgccgacag gcttctcttc actgccgcca tccggcccgg cgataccact
     5761 aatagacacg gcataatcag cacgagccgc tttcagtgca cctatcgcca tttccaccac
     5821 gacgggttca ctcaccgcgc catgctgcgc cagcgtctct tcgcgtacgc cgatcatctg
     5881 cgctttggct tcgttactgt aggtgacaaa tccgcgttca aaccaggcgg agctaccggc
     5941 aatatcggta atcactttcg ctacccaacc accggtacaa gactcggcag ttgttacggt
     6001 tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt aactgcatca gttcactgtc
     6061 agtcatcaca acctcagtca gtcaaaaatt tatgccggac aagatagcac tttcgtcagg
     6121 aagatggtgt tggcgttgca attttacgag gaggattcag aaaaaagctg attagccaga
     6181 gggaagctca cgcccccctc ttgcaaatag ttactgcacg cgcgccagcg ccacggcttg
     6241 tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg ttataacggg taatggtgta
     6301 gaagttcggc agaccgtacc agtactggta accggtaccg acatccagac gcagcaggct
     6361 ggcttgttga tggttgccca gcggctgctg tggcgttaaa cctgccgtgg caagctgcga
     6421 aatgctgtac ctggttttga agccatttgg caagcctgga gcctgaccgt ttgccattac
     6481 cgcgacctga tcgcctttca cccagccgtg cgctttgaaa tagttcgcca cactaccgat
     6541 cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg ctgaaatcta ccgcatattg
     6601 tttgtaagac gacggcataa actgtccgta gcccatcgcc ccggcaaagg aacctttcag
     6661 attgagcgga tcgtcctgct cgtcgcgcgc catcaacagg aaggtttcca gctcgccaga
     6721 gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt gccagcgcat cgaggatgcg
     6781 agttttcccc atcacgcgcc cccagcgggt ttcaacgccg ataatcccga cgataatttc
     6841 cggcggtacg ccatacacct gccacgcgcg attcaacgca tcttcatact gattccagaa
     6901 aaccacaccg ttctgcacgt tgtccggcgt aataaatttt ttgcgataac gcagccatgc
     6961 gccgttcgga cctgatggcg gtttcaccga tgtggttggt gcctggttat ccatcagccg
     7021 cagtaccgaa tccagacgct tcgcctggga gagaatttcc tgcaactgct gacgatcgaa
     7081 accgtgtttg ttcaccattc tgtcgatgaa ctgctgggca ttcgggttat tagcgaaatc
     7141 gccgcccatc tgcatcacat tgtgctgagg ctcaagcagg aagccgccag acggcgttcc
     7201 ggtggtcgtt tcagtctcag taggttttgg cttgctgcta caggcggcaa gcaacacaaa
     7261 aaggggaagc aatgttacat aacgacgctt gaacatgagg ggtccattta acagattcaa
     7321 ccaggggcaa gtatggtaaa gcatcacgcc ccgcacaagg aagcggtagt cactgcccga
     7381 tacggacttt ac
//
LOCUS       DAZSBF010000206         7366 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZSBF010000206 DAZSBF010000000
VERSION     DAZSBF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.210"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            189..1253
                     /gene="ulaG"
                     /locus_tag="ACHXWD_003461"
     CDS             189..1253
                     /gene="ulaG"
                     /locus_tag="ACHXWD_003461"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR7622280.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1361..2116
                     /gene="ulaR"
                     /locus_tag="ACHXWD_003462"
     CDS             1361..2116
                     /gene="ulaR"
                     /locus_tag="ACHXWD_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR7622281.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2113..2862)
                     /gene="yjfP"
                     /locus_tag="ACHXWD_003463"
     CDS             complement(2113..2862)
                     /gene="yjfP"
                     /locus_tag="ACHXWD_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR7622282.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3044..3373
                     /gene="bsmA"
                     /locus_tag="ACHXWD_003464"
     CDS             3044..3373
                     /gene="bsmA"
                     /locus_tag="ACHXWD_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR7622283.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3522..3797
                     /gene="yjfN"
                     /locus_tag="ACHXWD_003465"
     CDS             3522..3797
                     /gene="yjfN"
                     /locus_tag="ACHXWD_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR7622284.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3914..5539)
                     /locus_tag="ACHXWD_003466"
     CDS             complement(3914..5539)
                     /locus_tag="ACHXWD_003466"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR7622285.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5623..6786)
                     /locus_tag="ACHXWD_003467"
     CDS             complement(5623..6786)
                     /locus_tag="ACHXWD_003467"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR7622286.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6789..>7366)
                     /locus_tag="ACHXWD_003468"
     CDS             complement(6789..>7366)
                     /locus_tag="ACHXWD_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR7622287.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1927 a         1962 c         1799 g         1678 t
ORIGIN      
        1 atttatttaa attcatttag ttaatttaac tacacattcc attaaatctt tccacactca
       61 aaatcaacaa caacttccct ggcaaaaagt caaaaaatgg tcattgcctt cctgaaaaaa
      121 tagtgtcagg attaatcaaa accaatcaca tattgattcg aattgaacta tcatgaaggt
      181 aaatggcgat gagtaaagtg aaaagtatca cccgagaatc ctggatcctg agcactttcc
      241 cggagtgggg tagctggctg aatgaagaaa ttgaacaaga gcaggtcgcc cctggcacat
      301 ttgcgatgtg gtggctaggc tgcaccggga tctggttgaa atcagaaggt ggcaccaacg
      361 tttgcgttga tttctggtgc ggcactggca aacaaagtca cggtaacccg ttaatgaaac
      421 agggtcacca gatgcagcgc atggctggcg tgaaaaaact gcagccaaac ctacgcacca
      481 ccccgtttgt tcttgatccg tttgcgattc gccagatcga cgcggtactg gcgactcacg
      541 atcacaacga tcatatcgac gttaacgtcg ctgctgccgt gatgcagaac tgtgctgatg
      601 acgtaccatt cattggaccg aaaacctgcg ttgatttgtg gattggctgg ggcgtaccga
      661 aagagcgttg catcgtggtc aaaccgggcg atgttgtaaa agtgaaagac attgaaattc
      721 atgcgcttga cgctttcgac cgtactgcac tgatcaccct gcctgccgat caaaaagcgg
      781 ctggcgtact gccagatggc atggacgatc gcgcagtaaa ctacctgttc aaaacccctg
      841 gcggttccct atatcacagt ggcgattccc actactctaa ctattatgcg aagcacggta
      901 acgaacatca gatcgatgtg gcgttaggat cgtatggtga gaacccgcgc ggtattaccg
      961 acaaaatgac cagcgccgat atgctgcgta tgggtgaagc cctgaatgcg aaagtggtta
     1021 tcccgttcca ccacgacatc tggtcaaact tccaggccga tccgcaagag atccgcgtgc
     1081 tgtgggagat gaaaaaagat cgtctgaagt atggcttcaa gccgtttatc tggcaggttg
     1141 gcggcaaatt tacctggccg ctggataaag acaacttcga gtaccactat ccgcgcggtt
     1201 tcgatgattg cttcactatt gaaccggatc tgccgttcaa gtcattcctg taatctgtcg
     1261 tttataccgg atagcgtgga gaagttcgcg ctatccggcg tagtatttcc tagctatttc
     1321 aaatatcatc taaaaaaatc aaattttatc ggaatagctc atgactgaag cacaaagaca
     1381 tcaaatcctc ctggaaatgc tcgcacaatt gggctttgtg accgttgaga aagtcgttga
     1441 gcgtctggga atttcgcctg ccactgcgcg acgcgatatc aataaacttg acgaacgcgg
     1501 caaactgaaa aaggtgcgca atggcgcaga agctattacc caacagcgcc cgcgctggac
     1561 gccgatgaat ctgcatcagg cgcagaatca cgatgaaaaa gtacgtattg ctaaagcggc
     1621 ctcgcagctg gttaatccgg gcgaaagcgt agtcatcaac tgcggctcca ccgcgtttct
     1681 gcttgggcgg gaaatgtgtg gcaagccagt gcaaatcatc actaattatc taccgctggc
     1741 aaattacctg atcgatcaag aacatgacag cgtgatcatt atgggtggac agtacaacaa
     1801 aagtcagtcc atcactttaa gcccgcaggg cagcgaaaac agtctctatg ccgggcactg
     1861 gatgtttacc agcggaaaag ggctgaccgc agaagggttg tataaaaccg atatgctgac
     1921 agcaatggca gagcagaaga tgctgagcgt ggtagggaaa ctggtggtac tggttgatag
     1981 cagtaagatt ggcgaacgcg cgggaatgct ttttagccgt gccgatcaaa tcgatatgct
     2041 tatcaccggc aaaaatgcta acccggaaat cctgcaacaa ctggaagcgc aaggggtcag
     2101 cattctgcgt gtttaaagat gctggcggaa aaatgtcacg gcagcatcta acgcctcagg
     2161 cgtaatgcgg tgacgcacgc ctggctgcca tgaacaggtt agcagtttat cccgtcccgt
     2221 ctcgcttaag gcctgctgca aacgtagtga ttcgtcggca ggcacaacgt catcgtcgag
     2281 gccatgccac agcagtagag gtctgtcccc aagttgttcc aggtggtttg tcgcttccca
     2341 ctctgccagc ggcgcgataa tgttattgaa ttcattctgc tgtgctgccg tttcaggtat
     2401 cagcggtgga aacagtgaac gggcgagcga tgtaaaatag cccgatccca tcatgctggc
     2461 ggtacatttc accgtggggt ggcgagcggt aattcccagt gccgtcatcg cgcccatcga
     2521 cgcgccaccg actgccagac ggtcatcaag cagccagttt tcttcggcta ttgccgcacg
     2581 taaagtagtg aattcctgca tactttgtag caagatttgc cagaattgat ttaaccgccg
     2641 cgcttcgtcg ccactaaaac ggctaccgtg atcgggcgca tccggcatga tcacccgcaa
     2701 accagcctgc gccagcgcaa cggcaaaata gctatacacc aggctggatg aagtaaagcc
     2761 gtgataaaaa attacgcacg gtaacggggt atctttttgc ccgacaggat aagcatgaag
     2821 aacgggaata tctgccagct cgcgtgattc tatttcaatc atctgtctct cctttttgtc
     2881 tggtatgaca tgaagaaatc cttagcacaa gtttctgctg gcggtattag ttcataatgt
     2941 tgagatgtgg gttacgcttt cgtcacgttt tcattcgaaa atcgcgtacc aggtaacaat
     3001 tcgggaacat tcccccaaag ttaaattaat aaggcactac actatggtta gcaggaaacg
     3061 taatagcgtt atttaccggt ttgccagttt attattggtg ttgatgttaa gtgcctgtag
     3121 cgcactgcaa ggtacgccac agccagcacc gccagtaacg gaccatccgc aagagattcg
     3181 ccgcgaccag acgcaagggt tacagcgaat aggtagcgta agcacgatgg ttcggggttc
     3241 tccggatgac gcattagcag aaatcagagc aaaagctgtc gctgcaaaag ctgattatta
     3301 cgttgtcgtc atggtggacg aaaccatcgt gacaggacag tggtattcac aagccatttt
     3361 gtaccgtaaa taaatcagac aacttacata gtcagcttta cattgctttg cgtccctatg
     3421 cgtttacctg tgcacaataa attaccggct ggaagcctgg gcgtttatta ccgcgatgga
     3481 atgccctgca acatgtgggg aaacgaaaaa tggagctgac gatgaaacaa ttacttgcct
     3541 caccctcgct gcaattagtg acttatcctg cgagcgccac ggcacagtct gcagaattcg
     3601 ccagtgctga ctgcgtgacg ggcctgaatg aaataggtca gatctcagtt agcaatattt
     3661 caggggatcc gcaggacgtg gaacgtatcg tggcgttaaa ggccgatgaa caaggtgcgt
     3721 catggtaccg cattattacc atgtacgaag accagcagcc tgataactgg cgcgtacagg
     3781 cgatactgta tgcatgagtg tcatctacag caaatgaaca atgccggatg cggcgcgagc
     3841 gccttgtccg gcctacaatt ttcacatatt tcattgagtt aatcgaaacc agcgtcgcat
     3901 cagtcgtata cgtttacaaa cacactcccc ccgtcgcccg cagcaataaa tcattctgga
     3961 tctgctctga caaccgtacg ccgccgcgcg tatctaacat cacctgacac cacgcctgcg
     4021 ccattggcgg agaagcatat ttcaacattt gcgcaccgca gcccagcagg aatagctgat
     4081 gagtaatctc tcgccccagt tcttcagctg gcttacgcag ctgctgctgt aaacgacgaa
     4141 ccgcgcgatc aaaatagcga tcctgccctt tcacttccac aaatgcttcc gacaataagt
     4201 cgtatacgcc cgcttgctta ttgagaacgc gcaacacatc caggcacata atattgccgg
     4261 aaccttccca aatactgttt accggcatct cccggtaaag ccgcggcagc tcgctctcct
     4321 cgcaataacc aatgccgccc agcacctcca tcgcttcggc cacaaacggc atacctcgtt
     4381 tgcagatcac aaattttgcc gcaggcgtaa ataaacgcgc ccacagggct tctttggcat
     4441 cggcgcgccg gtcccacgct cgcgcaagac gaaacagcaa cgccgtttgc ccttcaagct
     4501 gaagtgccat gcgacttaag acatgacgca taaggggctg ttggatcaat ggattaccaa
     4561 aaacatggcg ttgatgtgca tgataaatcg ccagcgaaaa cgcacggcgc atcatggcat
     4621 ggctacccag ggcgcaatca aaacgcgtca tcccccccat tttcaggatc agacgaattc
     4681 cttccccttc ctgccccaat aaccagccaa tggcatcctg aaactccact tcacaactgg
     4741 cgttagagcg attacccagc ttatctttca gccgttcgag gcgaatcgcg ttgcgttgcc
     4801 cgtcaggcaa aaagcgcggc acaaaaaaac aggacagacc gcccgtggtc tgcgccagca
     4861 ccagatgcgc atcgctttgc ggaaccgaga aaaaccattt atgccccacc agccgataag
     4921 agccatcttc caaacgctct gcacgggtgg tgttgctcat gacatcggaa ccaccctgct
     4981 tttccgtcat tcccatgcca atcaacaaac cgcgtttttg cccacctggc aataagtgag
     5041 aatcgtagcg atcgctcagc agcggcgtgg tccagtcctg aaacggcgtg ggtaacatct
     5101 gtaacaacaa tggcgtggcg gcaaaggtca tcgttattgg acataacgac ccagcctcaa
     5161 cctgcgcatg taacataaaa cgcgccgcgc gcgccacaaa tgcgccggag cgagcgtctt
     5221 cttcccaggc aagattgtgc acccgattgg tacataacac ctgcatcagc aggtgccatg
     5281 cgggatggaa acgaacatcg tccagacgcc gcccctgggc atcgtagcgc aacagttcag
     5341 gcgggtttac attcgccagc cgccccagtt caagggattc agccgttcct aactgctgac
     5401 caatactggc tagaaaatcg ctatcccagc cagcaccttc acgcgttacc gcttcgcaaa
     5461 gcgcgccatc agacaggtat aagttgctgt tatttaatgg tataggttga ttaaaaacgg
     5521 tgtgagtttg ccagtgcact gtgtctccct ccatcaatgg cagtcaccat tagtatggcc
     5581 actgccatgg attcatgaca gaaagagatt gctaaaacat tcttatccag caatgtaatg
     5641 cggaacgaaa cgtgaggtgt ctttggtgat cagtgtgtta tcttcacgga tccccattcc
     5701 gcacgcttca tcatcgacaa tccagctatc gatgagtgtg tagctatcgc caaatcgcgg
     5761 cagaggttga aacgcctggt agatcatcgg ttcatcggcg taatcaccat cagcgtggtc
     5821 aacgacgtta ttctgaccgt cgaaaatggt gacgtttccg ccttcgcgcg agtagatcgg
     5881 tttacgcaca tagctttcgc cagcggcaat ctgcgatttt tcaccctcaa accaggatgc
     5941 aagaaggtta ggatgaccag ggaagaagcg ccaaagcagc ggcattagcc ctttattact
     6001 caagatactt ttccataacg gctccaccca ttgctcgcga cgcttgcaca gcagcggacc
     6061 gttatcgtca cgcatcatcc actccagcgg atacagctta aatgcacgct ggatgacatt
     6121 atcatcaaga tcggtcagta caccgccgac gcccaaaccg agatcttcaa tgtagataaa
     6181 ccgcgactcc tgccctgcct gctgggcgca gtcctgcaag tacagcacgg tagtcctgtc
     6241 ttcatcggtg tcctgacagc agcagaaata aaacggctcc cggctataaa gctcgctaaa
     6301 gcgtgaaatc aggcgttcct gaatggcatt gtactgatcg gcatcacgcg gaataatgcc
     6361 gctgcgccgg gcatcctcca gccacaacca ctggaaataa gccgactcat acaacgaagt
     6421 cggcgtatcg gcgttgtact ccagcagctt caccggcgca ttgccacacc aggcaaaatc
     6481 catccggcca tacagcgaag gatcgctggc gtgccagctt tcggcgatca catcccagta
     6541 caacggcggg atcgccagtt gcgtcaggat ctcttcgtct ttaaccgcgc gatccaccac
     6601 ctcaaggcac atctgatgca gttccgcagt tggtttttcg atctgctctt caatctggcg
     6661 cagagtaaaa cggtaagccc gactctcgtc ccaatagatt tcattgtcga tgatatgaaa
     6721 gtcgaaaccg ttgtcagcgg caatttggtc cagatcccgc cgcacaggaa cgttgtgtct
     6781 cagcatgatt agcctcccca atgcccacgg gcgctggaag aacgaccata gccgccgcgc
     6841 gaaacggttg acgctttttt ggtggtaaag ccgcctgaag agtaagactc ttttttgcca
     6901 gaaccggagc gccaggagta atcaccggaa gtgttgcgcc agacggggcg ggaagcaaat
     6961 gaggatccgc cgctgttata gacgaacggc tcatcgcgat ctttacgaat aacccggctt
     7021 aacaaaaatc cagaaacgac cgggatccaa ctctgttcaa cattgtcgta gtaggaattt
     7081 tcgtacttag actgacagtt ctgctgagtc atattcttcg gaacatcggc ataaaatgcc
     7141 gttttggcgt tgttccagcc acgcgcgcaa atgtcggcat tattaccgtc atcaatacaa
     7201 tcctgcactg tcgcgtaaaa cgttccgtcg ccgtcgttat cgacatcgct actatcgcca
     7261 caacctttca atacgaaaaa agcggcaccg cccattagcg ccaacgttaa gtatttttgt
     7321 gcgtagcgat tgcgacgcgg ttcaaaagga ttattcggtc taccaa
//
LOCUS       DAZSBF010000207         7265 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZSBF010000207 DAZSBF010000000
VERSION     DAZSBF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.211"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..4519)
                     /locus_tag="ACHXWD_003469"
                     /pseudo
     CDS             complement(1..4519)
                     /locus_tag="ACHXWD_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4539..5000)
                     /locus_tag="ACHXWD_003470"
     CDS             complement(4539..5000)
                     /locus_tag="ACHXWD_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR7622288.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6929)
                     /locus_tag="ACHXWD_003471"
     CDS             complement(5028..6929)
                     /locus_tag="ACHXWD_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR7622289.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1379 a         2206 c         1958 g         1722 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccttggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcgggcc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca gattgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg ttattcagga
     7141 caggtacgca tatcacaacg agtaacaaaa acgcaaagtt aaaccaatat tcataatata
     7201 tttttattca cagcaataaa taatccaatt aacattatta aaatgaatga ttgcataaga
     7261 aatat
//
LOCUS       DAZSBF010000208         7246 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZSBF010000208 DAZSBF010000000
VERSION     DAZSBF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.212"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(124..870)
                     /gene="cutC"
                     /locus_tag="ACHXWD_003472"
     CDS             complement(124..870)
                     /gene="cutC"
                     /locus_tag="ACHXWD_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR7622290.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(884..1450)
                     /locus_tag="ACHXWD_003473"
     CDS             complement(884..1450)
                     /locus_tag="ACHXWD_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR7622291.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1666..3402
                     /gene="argS"
                     /locus_tag="ACHXWD_003474"
     CDS             1666..3402
                     /gene="argS"
                     /locus_tag="ACHXWD_003474"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR7622292.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3444..3488)
                     /gene="yecV"
                     /locus_tag="ACHXWD_003475"
     CDS             complement(3444..3488)
                     /gene="yecV"
                     /locus_tag="ACHXWD_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR7622293.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3579..3941
                     /locus_tag="ACHXWD_003476"
                     /pseudo
     CDS             3579..3941
                     /locus_tag="ACHXWD_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(3945..5015)
                     /locus_tag="ACHXWD_003477"
                     /pseudo
     CDS             complement(3945..5015)
                     /locus_tag="ACHXWD_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(5008..5943)
                     /gene="flhB"
                     /locus_tag="ACHXWD_003478"
                     /pseudo
     CDS             complement(5008..5943)
                     /gene="flhB"
                     /locus_tag="ACHXWD_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
     gene            6096..7115
                     /locus_tag="ACHXWD_003479"
     CDS             6096..7115
                     /locus_tag="ACHXWD_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR7622294.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT         1912 a         1869 c         1835 g         1630 t
ORIGIN      
        1 caacagatca cggttatcgt tcgtttttta tactgctcag ggataaaaaa aggcccctgt
       61 tgaaattgca ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa
      121 aaatcatttg gcctgatggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc
      181 tacgatataa cgcgaatact cgtccgcgtg ttcatctgat gacatggaca atccttgatt
      241 acgataacgc atcggtgagg cttgccacgc tcccgcggag ctatggactt ccagcactcc
      301 ggcatcgagg aagtggtgca ggttttctgc acggactcct gctccggcca taatgattgg
      361 agcatcacga tgggcaataa gttccataat ttttgataaa ccttgcagtg cgtctgattt
      421 ttgccctgat gtcagtaccc gggcaatgcc caattccgca agattattga gtgtatataa
      481 agggttagcg cacatatcga aggcgcgatg aaaagtcact gccagcggac cggcagcagc
      541 cattattttt tccattcgtg gcatatcgac attcccgtca acatcgagaa cgcccgtcac
      601 cagtccagga aaacctaatt cgcggaccgt gcgcacatcc tcaagaatgg cggcaaactc
      661 accgtcgctg tagcaaaaat caccaccgcg tgggcgaatt atcggatgca cagggatcgt
      721 cacccgctgg cgcacggatt tcagtacccc caacgacggc gttaagcccc cctcttttgg
      781 ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc gccgttagtg cacattccat
      841 gctgtaacag caaatttcca gtaatgccat ttttgctcct taattacgct gactgctcgc
      901 tggcaacgat ctcttcaatg gaccacggat gaaacttaat agttgttttg ccatcggtga
      961 ccgccagcgt cggattcggt aatcgctcat gctcaccttt tggtgaactg gttttaactg
     1021 agatccccgg taggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg
     1081 tttctggatc acccagcaga acgatttcaa tatgttccca accttcgtgt gggtaacgtt
     1141 tttccccggg ccacggtagc tccacaatag aaaactgcca gtgcgcaacc tgtaccggtt
     1201 catgcaattt aaacagacaa atcggtctgc cattgatcat attttctgac aaaagctcgc
     1261 cacactgttc aaacccgcga cgccagcgtt cagcagtggc gttttgatgg caacgcaaag
     1321 aaatgtgatc ggcagtcagc ggagtgatat tcaaccccag acggagggaa agttcatcta
     1381 atgcgtgggt aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc
     1441 agttcgccat aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgc
     1501 gccaaccaac cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca
     1561 cgcgcacgtt gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac
     1621 tggtgcgggc aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct
     1681 cttctctcag aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa
     1741 ccgcaggttc gtcattcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg
     1801 gcagttgcta aaaaactggg tatgacaccg cgacaattag cagagcaggt gctgactcat
     1861 ctggatctta acggtatcgc cagcaaagtt gagatcgccg gtccaggctt tatcaacatt
     1921 ttccttgatc cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc
     1981 ggtgtttcta cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg
     2041 aaagagatgc atgtcggtca cctgcgctct accattattg gtgacgcagc agtgcgtact
     2101 ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggcactcag
     2161 ttcggtatgc tgattgcatg gctggaaaag cagcagcagg aaaacgccgg tgatgaaatg
     2221 gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaagatgaa
     2281 gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc
     2341 cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat
     2401 cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta caacccaatg
     2461 ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg
     2521 accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt
     2581 cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc gaaatatcgt
     2641 tatgaaacac tgcatgccga tcgcgtgctg tattacatcg actcccgtca gcatcaacac
     2701 ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg
     2761 gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa aacccgcgcg
     2821 ggtggtacag tgaaactggc cgatctgctg gatgaagccc tggaacgtgc acgccgtctg
     2881 gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc taacgcggtt
     2941 ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga ctacatcttc
     3001 gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac
     3061 acgcgtgtat tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact ggctgcagct
     3121 ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa
     3181 gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac
     3241 gatctggctg gtctgttctc tggcttctac gagcactgcc cgatcctcag cgcagaaaac
     3301 gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac gctaaagctg
     3361 ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgatttt tcgtgagagt
     3421 aaagcctgat cagggttacc tgatcaggct tttttattgc catctaaatg tattctgaaa
     3481 atggacatgc cattgttttc tgactgttgg ataagaggcc agaagcgtaa tatccggccc
     3541 cagggaaacg ataacggttg aatttaagga ataccgcagt gtttaaattt cttgtattaa
     3601 cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagaa aacgaccatg
     3661 acatttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact gacattaacg
     3721 tgatcaaatc atgcctgcga caagaataca atctcgtcga tgcgcaatta aataaagcct
     3781 atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgtaaaa aaacctgata
     3841 ccgaacaact taacttgctt aaaaaatcac agcgagcctg gctggatttt agggacaaag
     3901 aatgtgaatt aatcctttca aatgaggacg ttcaggattt aatgtacatc gttccacgtc
     3961 gcttcgacaa cggtattatt ctctgccatt tttaccggtt tgggttcggc aggcgctttt
     4021 tgttcgcgtc cgcgtatcca ccaggccagc ccgagcaatc cggcagtgaa cagcaaaaat
     4081 accaggttcg gcattccagg caccaggccg agtaaaccga gcacggcggc gcttaacaac
     4141 ataacgcttg ggttactgaa aagctgattc accatctgct cgccaacatc ctgatcggtg
     4201 ctgacacgcg taacgatgac ccccgcggcg gtaaaaatca ccagcgccgg aatttgtgcc
     4261 accagaccat cgccaatggt caatagcgta taactttccg ccgcgtgtcc catgctcatg
     4321 ccatgttgca gcacgccgac cagcaacccg ccgacaacgt taatgaccat gatgaggatc
     4381 ccggcgatgg catcgccgcg aacaaactta cttgccccgt ccattgagcc gtaaaaatcg
     4441 gcttcctgag tcacttcgga gcggcgtttt ttcgcctcat cttcaccaat caatccagcg
     4501 ttaaggtcgg cgtcaatcgc catctgctta cccggcatac catcgagaac aaagcgcgca
     4561 cccacttctg cgatacgccc ggcacctttg gtaatgacca taaagttgat gatcacgaga
     4621 atgacaaaca ccacgatacc gatagcgaaa ttgccaccaa cgaggaagtg accgaacgct
     4681 tcgaccacct tccctgccgc cgccgcgccg gtatgccctt ccatcaaaat gatacgggtt
     4741 gaagccacgt taagtgccag acgcaacaac gtagtaaaca acagaatggt cggaaacgca
     4801 gcaaactcaa gcgtgcgctg ggtaaacatc gccaccagca acaccatgat cgacaaggca
     4861 atattgaagg taaacaacag gtcgagtatg aatgcgggca gtggcagcac catcatcgac
     4921 aagatcaaca ggatcaaaat cggtccggca aggatctgcc attgtgtcga tttcaggttt
     4981 gcgggcaggc gcagcatcgc ggccagatta ctcatgggtc ggtttctcgt taataaaatc
     5041 cagggcttcc ggcaccggaa gatgagtagg ttgtacaggg cgctgtccac cagccagacg
     5101 ccagcgtttc agttgccaga cccaggccag cacttccgcc accgcggcgt acagttgacc
     5161 cgggatttgt tgaccaatct ccgcatgtcg atacagcgct cgcgccagcg gcggcgcttc
     5221 aagcgtcggg acgttatttt cagcgccaat ttcacgaatg cgcagcgcga ccagccctgc
     5281 acctttagcg accactttcg gtgcgctcat tttgttttcg tcatactgca acgctaccga
     5341 atagtgggtc gggttattga caatgacatc agctttcggc acatcggcca tcatccgacg
     5401 ccgtgcagca gctcgctgca tctgacggat ccgcccttta acatgagggt caccttcgct
     5461 ttgtttgaac tcatcacgaa tatcctgccg cgacatacgc agctttttca ggtggctgaa
     5521 gatttggaaa aagacgtcaa atcccaccat tggaatgaca ccaagcacca ccagcagtgc
     5581 gcatagccca accagatcca tcgcattacc catggcggta gtcggagact cggccattaa
     5641 gcgcatcatc tgcggccagt gatgccagag ataaaacccc gtcacgctgc caaccaggat
     5701 ggttttcaaa attgccttaa gcaactccgc gccagtctgt gccgagaaca tccgtttaat
     5761 gcccggtagc gggttgagtt tggaaaactt cggctgcaag gatttgccgc taaataccag
     5821 cccccccagc atgaccggag aaatgagcgc caccagcacc acgccgctaa tcagcggcag
     5881 cagcgccagc atggcttctc tgatcagcag aataatctgc ccgaggatca gattcgggtc
     5941 attgaaaatg taatgaacgc atcccacaca attaaacacg gagcagtatg atggttaaaa
     6001 gggtgaaact ggcaggtgtg gagcctcgca gatttccggc atcatagtgc ccaatacggg
     6061 atgcataaag ctccctcaga ctggagactc cacaaatgaa atatacaccg gttggcgttg
     6121 atatcgcaaa acatgtcatt cagattcact tcatcaatga gcacacaggt gaagtggttg
     6181 ataaacagtt gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga
     6241 ttggtatgga ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc
     6301 ataaagtccg gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg
     6361 atgtgatgga tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg
     6421 taaaaacaga agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga
     6481 agttccggac cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca
     6541 tccacaaagg ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag
     6601 agagactgcc accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc
     6661 tggactcact gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa
     6721 cctgtaagcg gttgctggat attcctggcg ttggaccact tattgcgacg gcagcggtgg
     6781 ccaccatggg ggaagcatca gcgtttaaat cggggcgaga gttcgccgca tatgttggtc
     6841 tggttccaaa acaaactggc tccggaggga aagtacgtct gctggggata agcaaacgtg
     6901 gtgacactta tctcaggaca ttatttatcc acggtgcaag agcggtggca ttagtagcta
     6961 aagagcctgg cccgtggata accgaactga aaaaacgtcg tccagccagt gtggcaatcg
     7021 tcgccatggc aaacaagctg gcacgaacag tatgggcgat aaccgcccat gaccgtaagt
     7081 atgacaggaa ccacgtcagt atcagaccat attaatcgct gataccatta aacaatgaac
     7141 tcttaacaaa agggtgaatg ctgaaaggtt gctatggcgg ccagagtgat gacaaagaca
     7201 ggtaagaccg tgactcacta aacctgaaca gtattttggg cttgaa
//
LOCUS       DAZSBF010000209         7245 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZSBF010000209 DAZSBF010000000
VERSION     DAZSBF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.213"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..571)
                     /locus_tag="ACHXWD_003480"
     CDS             complement(<1..571)
                     /locus_tag="ACHXWD_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR7622295.1"
                     /translation="MANENLDIDSKTAEELYEWYLDDKLIINRRYQRKLVWSLEEKTA
                     LISSMTQQYPIPLLLFVSIDNKREILDGMQRLEALMSFIEQRFSLNGKYFNLDAIALT
                     KQLKDSGKLQQKEPVLSRESSTLIARYRFAISEYSSTEDHIDEVFRRINSNGKILSKQ
                     ELRSAGCVSNFSELVRKISTIIRGDTTHSD"
     gene            complement(856..1188)
                     /gene="pemK-mt"
                     /locus_tag="ACHXWD_003481"
     CDS             complement(856..1188)
                     /gene="pemK-mt"
                     /locus_tag="ACHXWD_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFR7622296.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(1190..1447)
                     /gene="pemI"
                     /locus_tag="ACHXWD_003482"
     CDS             complement(1190..1447)
                     /gene="pemI"
                     /locus_tag="ACHXWD_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFR7622297.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(1540..2193)
                     /locus_tag="ACHXWD_003483"
     CDS             complement(1540..2193)
                     /locus_tag="ACHXWD_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFR7622298.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(2291..2431)
                     /locus_tag="ACHXWD_003484"
     CDS             complement(2291..2431)
                     /locus_tag="ACHXWD_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622299.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSYETTECRLP
                     CR"
     gene            complement(3132..3989)
                     /gene="repA"
                     /locus_tag="ACHXWD_003485"
     CDS             complement(3132..3989)
                     /gene="repA"
                     /locus_tag="ACHXWD_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR7622300.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAAAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3982..4056)
                     /gene="tap"
                     /locus_tag="ACHXWD_003486"
     CDS             complement(3982..4056)
                     /gene="tap"
                     /locus_tag="ACHXWD_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR7622301.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(4301..4549)
                     /locus_tag="ACHXWD_003487"
     CDS             complement(4301..4549)
                     /locus_tag="ACHXWD_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR7622302.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQVMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(4834..4983)
                     /locus_tag="ACHXWD_003488"
     CDS             complement(4834..4983)
                     /locus_tag="ACHXWD_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7622303.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            complement(5230..5703)
                     /locus_tag="ACHXWD_003489"
     CDS             complement(5230..5703)
                     /locus_tag="ACHXWD_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622304.1"
                     /translation="MKYDDVSELMIKREHNILIVGNSASHIDKFFKGYSIPGSKYYDF
                     TQINSDSDVERNEDAVSFIRDAMNSEEQTIIFNCAGWPDLGGGSAVSQFAMMVRKFGK
                     QLIVAVSEKDAIKLKDNFDIIGMLSYGKENFIAMSHTKNELTGGKRRYRIKNGCK"
     gene            complement(5858..6448)
                     /locus_tag="ACHXWD_003490"
     CDS             complement(5858..6448)
                     /locus_tag="ACHXWD_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR7622305.1"
                     /translation="MKLIIFILIVLIIAALLIRIILKSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(6486..6695)
                     /gene="hha"
                     /locus_tag="ACHXWD_003491"
     CDS             complement(6486..6695)
                     /gene="hha"
                     /locus_tag="ACHXWD_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFR7622306.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(6741..7202)
                     /locus_tag="ACHXWD_003492"
     CDS             complement(6741..7202)
                     /locus_tag="ACHXWD_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR7622307.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
BASE COUNT         1857 a         1844 c         1600 g         1944 t
ORIGIN      
        1 tatcgctatg tgtagtatct cctcttataa ttgtcgatat tttccttact aactctgaaa
       61 aattactaac gcaccccgca cttcgtagtt cttgttttga taaaatttta ccattggaat
      121 ttatgcgtcg aaacacttcg tcaatgtggt cctctgttga agagtattca gatatagcaa
      181 atctatagcg agcaattaat gtagaacttt cgcgggatag cactggctct ttttgttgaa
      241 gtttaccact gtcttttagt tgttttgtta atgcaatggc atctaaatta aagtatttac
      301 catttaatga aaatcgctgc tcaataaaac tcattaaagc ttctagtctt tgcattccat
      361 caaggatttc tcttttgttg tcaatggaaa caaacaatag caaagggata gggtattgct
      421 gagtcattga tgaaatcagt gctgttttct cttcgaggga ccatactaat tttctttggt
      481 atcttcgatt tataattaat ttatcatcca gataccactc gtataattct tctgctgtct
      541 tggagtcaat atctaagttt tcattagcca ttagatgtat ctcgagtatt tagttaagaa
      601 actgataggt cagtatttgc atcaatgttc attgaatttg tatccgaagt caatctgatg
      661 taggaacttc aaaactgcgc aaaacacgca aatacgcaca agaaagtagg gctgcgcttg
      721 gaattcggaa aataaaccac atcaaaacta ctttttagcc aaataacgtt tgggaatcac
      781 cagaatggtg ggacaacagc ggttttagtg ccctaaatcg tacgttttca tccagttgcc
      841 cctcaaaccc catgttcaag tcagaatagt ggacaggcgg ccaagaactt cgttcatgat
      901 agtctccgga acccgttcga gtcgttttcc gccccgtgct ttcatatcaa ttgtccgggg
      961 ttgatcgcaa cgtacaacac ctgtggtacg tatgccaaca ccatccaacg acaccgcaaa
     1021 gccggcagtg cgggcaaaat tgcctccgct ggttacgggc acaacaacag gcaggcgggt
     1081 cacgcgatta aaggccgccg gtgtgacaat cagcaccggc cgcgttccct gctgctcatg
     1141 acctgcggta ggatcaagcg agacaagcca gatttcccct ctttccatgt cagatttcct
     1201 cctgaccagt cgccggtgca tccagccatt ctcgttcttc agctgatatt tcagcattcg
     1261 gatcacactg tgccagtagc tcagccagtg aatattgcgg gcgtctgtac ggctcaacaa
     1321 tcagccggcc attatcaatg accatgccaa cttcattatc tgtgcccaga gacagcgcat
     1381 tcagcagtgc cggtgggacg gtcagcataa ctgagccgcc aaccctcttc agtcgggtgg
     1441 tatgcattct tcacctccat aaaagttata tttaaatata acatccacta aaaaaacaca
     1501 ccaggcttta acgcacaatg tttaataaaa atataacttt caaccaaaca gtaaacccag
     1561 cgtggttgct tccatatgca gcaataccgg cagcagcagg ccaccacttc tgatcctggc
     1621 cactgatgta atcaacccca ccaggaacag ttctgccagt gtcagcaggt tctgatactg
     1681 gctgtgcgcg gcgacgaaca acaacgacgt tatcagcgcc cccagccaca tcgtccagca
     1741 gtaccgtgaa cggaagacgt tcagcataat cccccggaac agcgtttcct cattcaacgg
     1801 ggcaaggata aagatggtca gcaacgtcag gatcacgtca ggtatggact tatcggcaaa
     1861 aagtttcgtc ataaatggct cagcaggcag agccagcgcc ttaccgagca gaaatacacc
     1921 gacatacacc acggccatcg caccgaccag ccacggtacg ccaacgttgc gcagctgacc
     1981 aacgaccggt agcggagcta tccaacggcg gtataccagg aaaacacaca gcaggtacat
     2041 cagaacagta ccatgactga agaacaaata gttttttcct gatccataaa gcagaacggc
     2101 ctgctccatg acaaatctgg ctccccaact aatgccccag gcagccagca taaccagcat
     2161 aaactgcaga tattgattac gtgtttgaat cattgcatcg cctgtaaatt tttaacttgt
     2221 cctatttttg tcattaccac gtatatacac atgtataaca attcagatat cgttaccagg
     2281 atatgccgca tcagcggcat ggaaggcggc actctgttgt ttcatatgat acaggagtaa
     2341 aaccgccgaa gcccggcgta agccggtact gattgataga tttcacctta cccatcccca
     2401 gccctgccag accatacccg ctttcagcca tgagagagct tctgtgcgcg gtcggagtgg
     2461 tcccgacgag ggtttacccg aagtcggggc gtatctccgc gttagcgggc cgtgagggcc
     2521 gcttacgagc gtgtactgag aacttccagc gagaagactg acagcgatga agatgtagtt
     2581 acaacattca taattaaaag cgactctgtt ccggcccttt gggccggggc ggggccgctt
     2641 ttcagttatg agggaggggc tttgtggttt cggttctgcg ctggaccggg gtttttctgg
     2701 aggttgtttt tgtgtgttgt aactaaagtg gctccggtcg gggcccgccg cttgcggtgg
     2761 gaggtgcata tctgtctgtc cacaggacag gcagtgaata ggttttcttt ttaaatgaat
     2821 gtaattaagt agtttaaagg agatataaac aggtgtttaa aagatacatt gcaccctgta
     2881 agactggcgg ctggcgcttt atgacatgaa cggttgtaac cttatgggga agtcccttgc
     2941 agttaaatgt ggataagcaa aattccccgt cgctgaggcg tattttgtat taaaaacagg
     3001 gggaatcgga tgctccagaa ggtggatgat gagattgttt tttgcatgcg acgctgtttt
     3061 tttgtgcacc ggcgggcttc aggcgtgcga atgcctccgg cgcgggccgg attattcaga
     3121 ggaggtcact ttcaggggga agctgtggcc agccggctgt aattacggtt acgtgacaga
     3181 atcatgcgct ccttcacacg acgctcaact tcgcgtttta ccgcctcacg attggcagtg
     3241 aagcgccctt ccgcgatttc gcgcgtcagc tgccgtttca ccagggtgac aatatcctga
     3301 cgttccctgt ccgcatcacg acgcgcacgg gcacgcttta ttccccggga cttaagctct
     3361 gtctgataac tgcgaaaacg ctcacgaaca aaacgccagg ctttcgctat cagttcatcc
     3421 atgcccaggg tatccagccc ctgctttttg cgttgtttgt tttcccatac cacacggctg
     3481 cggcgcgcgg cggccgctgc ctcctctgat acatcgaggg cagcaaacag tgcagatgtg
     3541 aacgtgatat cggtcggaat gtagcacccg ataagcgggt catattccgt ctggtaggta
     3601 atcagtccca gctctgacag gaacgtcagg gcccgggtgg cacgggtgat ggagagtttt
     3661 ccggcagcag actccgtcgc cagtccgcac tcaatggcca gcgtggtgat ggagcactgg
     3721 acgcggttgg ccagcgggtc atagtggaaa cacagcccct gcaggagcgc atcaatagcc
     3781 cgtcgacgca gcaccggtgg catgcgtcga cgcagaccac gcgaacgggc atgcgccaca
     3841 tgaatggcga aatcaaaacg ggaagtgaag cccaccgcct tttccatcag tttttcgcag
     3901 aacttcagcg ttccggcacc tttacggggt gtaaacaccg gattcgggtt ctttacctgg
     3961 cggtaatacg tttgttgaag atcagtcaca ccatcctgca cttacaatgc gcagaaggag
     4021 tgagcacaga aagaagtctt gaacttttcc gggcatataa ctatactccc cgcatagctg
     4081 aattgttggc tatacggttt ttagatggcc ccggtaatct tctcagtcgc caaacttgtc
     4141 gaagattatc ggggttttca cttttctggc tcctgtaaat ccacatcaga accagttccc
     4201 tgccacctta cggcgtggcc agccacaaaa ttccttaaac gatcagcaat ctatcacgca
     4261 caccagaaac aggcaagaat gtgatgtcgc tacattttag ttagctaaaa gcactcttct
     4321 gctttatcag ttcggcaatc atctcagcct gggtaacgcc ctcagcttca cacatcacct
     4381 gcaattgcgc ttttaacgca gcaggaatga acactctgag ttctttatgt gtagctcgtt
     4441 ttcttgccag ggatgcctgc tgacgttctg caagcgtcag aggattacct tttctgtatg
     4501 ctcgctttgt acctgatgag gaagtcactg cattttctgt ctgcgacatc tcgcctcctc
     4561 aatactcaaa cagggatcgt ttcgcagagg atactacagt tttttgaaat cagcgacttg
     4621 agaattgtga cgaagatctg tgttggcgca caaatcaacg gggattactg ttgtttaatg
     4681 tgatttttgt tgcgaaatgg ctgaaaaaac aggaaatgcc ttaacccggg agccggcccc
     4741 gggcgggacc ggctgtttct ggcatgtcgg gtgtctgccc ctgaaatacc tcacacttcc
     4801 ggaaagcggg gacttccgtc cccgcccggc agtttacttc cgtgcttcgt aggccagagt
     4861 tacctgcacc actgtatttc ccctgtgaat attcagctca cataaccgtt ccctgaatat
     4921 cagtgaaaaa cacaacaccg tggcgcacac ggcgagcaac ccgataaggg catacttcgt
     4981 cataaacttt gacctctctg caaggaagag gctacaatca gtagtgcaat gtacttcatg
     5041 cagacctcac gagttaatgg attaacaagt ggggtctttg catttctggt acctgcgtct
     5101 tgcctgaacc agaaaggctc agacacccgc cgcgataata ccacattccc tgttgctgac
     5161 tattacctgc attgcccgct ttttactaca gaagcacgaa ggcgtcactc cagccaataa
     5221 gtatgctttt tatttacatc catttttaat ccggtaccga cgttttcctc cagttaactc
     5281 gtttttcgta tgactcatgg caataaaatt ttctttacca tatgacaaca tcccaataat
     5341 atcaaagttg tcttttaatt tgatggcatc cttttcactt acagcgacaa taagttgttt
     5401 accaaatttt ctgaccatca ttgcaaactg tgatactgct gatccaccac ctaaatcagg
     5461 ccacccagca caattaaaaa tgattgtctg ctcttcacta ttcattgcat ccctgataaa
     5521 ggagacagca tcctcatttc tctctacatc tgaatcactg ttaatctgag tgaaatcata
     5581 atatttagaa cctggtatag aatacccctt aaaaaactta tcaatgtggg aggcagagtt
     5641 cccaacaata agaatattgt gttcacgctt aatcatcaat tctgatacat catcatattt
     5701 catatattca ccattattat ataaagtttg ggtacaggtg gttccggaat ttactctgtg
     5761 accaccattt cccgcttttt attacagaaa cgccgggagc ggggaaaact tgctttttcc
     5821 cgtttccggg gttggacaac tgagcaacgc gaaggcgtca gctacgatgt tccggggact
     5881 gctgatcagc ccctgttgta ttcagccatt ctccggtcat ctgcagcagt tttctggcat
     5941 cgtggcttct gagcaacgga ataccagcct gcctcagaac ctcttccaga agaatatccc
     6001 ggcgccggcg ttccggtcgt aaatggctgg catcatccag ctctactgct gcaacaatac
     6061 tgaacgaacg ccgttcaacg ataaccacat caacatgcca ctgagacacc atcctgaata
     6121 actgccacca ctggcgtgat cgtggccgga tattcccgtt cagctggacg atatccgcga
     6181 cccgtacctg cggacacaga taccaccgct tcatatccac aattctgaac agtcctttca
     6241 taaaactgac ttccctttct gtcagcagcg tcttctgacg ttgccagtat tcaccgccgg
     6301 acagtattcg ctctgcatca ccggtccgta tacctgccgc atgcaactgc atcagcaaag
     6361 gagaatgctg atttacactt ttgaggataa tccggataag taacgctgca ataatcagaa
     6421 caatgagaat aaaaataatc agcttcattg tttatctgct cctggtaatt aattattctg
     6481 aattattacc ttacatattt ccatatctct tttggtatcc tgtcatataa ctttccggtg
     6541 attaattccg ctaaccggtg gtctgctgct gaattaaatg attcacgttc actgtttaat
     6601 aaactgtctc tgtttttatg aataattttt tccagcgtat tgactgatga acatcgccgt
     6661 aactgataca gccattcctg ttttgttttc gccattttta ttcccgctga gaacagatga
     6721 atgataagat tgccatcgct ttatttacgg tgtctccata tccagggggg aaccggatca
     6781 gcatctgacc agagcccacg tttttgttgt ctggcttcgt tctgaaggac aggcagcacg
     6841 ggatctgtgt tgtactgctc atatacccag gcagccccgg cccggaccat aaactgatta
     6901 acattcattc cgtctggtgc ataaacctgc ccgagaatac gaccataacg atctcgctga
     6961 aaatatgtga cggtaaccgt ttttcctgca accagtgatt tcatcatgtc cgttgaccac
     7021 cggccataat cctgtttctt ttccggtgca tcaatattta ccagcctgat ccgcactgcc
     7081 ttccgtgagt ccatgacctc gatggtatca ccgtccagta cacgaacaac ccgtccatga
     7141 atatctgcac acaataccgg ccatgaaaag ataaataata ccagtggaat atatttcctc
     7201 atgttattct ccatatcaga taaatggcac ctgtatttca ggtaa
//
LOCUS       DAZSBF010000210         7241 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZSBF010000210 DAZSBF010000000
VERSION     DAZSBF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.214"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..197
                     /locus_tag="ACHXWD_003493"
     CDS             <1..197
                     /locus_tag="ACHXWD_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622308.1"
                     /translation="PEEEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQHMQRRE
                     EVNINVHRERGEDVEPGDDF"
     gene            197..5467
                     /gene="traI"
                     /locus_tag="ACHXWD_003494"
     CDS             197..5467
                     /gene="traI"
                     /locus_tag="ACHXWD_003494"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR7622309.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEIRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDI
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWEKNPDALALVDSVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRTATSEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            5487..6233
                     /gene="traX"
                     /locus_tag="ACHXWD_003495"
     CDS             5487..6233
                     /gene="traX"
                     /locus_tag="ACHXWD_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFR7622310.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            6288..6848
                     /locus_tag="ACHXWD_003496"
     CDS             6288..6848
                     /locus_tag="ACHXWD_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR7622311.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRRRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGETPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            6980..7192
                     /locus_tag="ACHXWD_003497"
     CDS             6980..7192
                     /locus_tag="ACHXWD_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR7622312.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
BASE COUNT         1643 a         1838 c         2431 g         1329 t
ORIGIN      
        1 aaccggaaga agagatggaa cagcaactgc cacccgggat cagtgaatcc ggtgaagtgg
       61 tggatatggc cgcttatgag gcatggcaac aggaaaatca tccggacatc cagcagcata
      121 tgcagcgtcg tgaagaggtg aacattaatg tgcaccggga gcgcggggag gatgttgagc
      181 cgggagatga tttctgatgt tgagtttcag cgttgttaaa tcggcaggca gtgccggaaa
      241 ctattacacc gacaaggata attactatgt gctgggcagc atgggagaac gctgggccgg
      301 tcagggggct gaacagcttg ggctgcaggg cagcgtcgat aaggatgttt ttacccgtct
      361 tctggagggc aggctgccgg acggggcgga tttaagccgc atgcaggatg gcagtaataa
      421 gcatcgtccc ggctacgacc tgaccttctc cgcccccaaa agtgtctcca tgatggccat
      481 gctcgggggg gataaacgtc ttattgatgc acataaccag gccgtggatt ttgctgtccg
      541 tcaggtggag gcgctggctt ccacacgggt gatgacggac ggacagtcag aaacggtgct
      601 gaccggtaat ctggtgatgg cactgtttaa ccatgacacc agtcgcgatc aggacccaca
      661 gttacacacg catgtggtgg tggctaatgt cacgcagcat aacggcgagt ggaagacact
      721 gagcagtgac aaagtgggga aaaccggatt ttccgagaac gtgctcgcga accggattgc
      781 gttcgggaaa atatatcaga gtgagttacg tcagcgcgtt gaggcgctgg gttatgaaac
      841 cgaagtggtc ggtaagcacg gtatgtggga gatgccgggc gtaccggtgg aggccttttc
      901 cagtcgttca caggctatca gggaggccgt cggtgaagac gcctcgctga aatcccggga
      961 tgtggcggca ctggatacgc gaaaatccaa acagcacgtc gatccggaaa tcagaatggc
     1021 tgaatggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc gtgacgcggc
     1081 ggatcagcgt gcggagatcc gcacgcaggc gcccgggcct gcttcacagg acgggccgga
     1141 tgtgcagcag gcggtgacac aggcgattgc cggattaagt gaacgaaaag tgcagttcac
     1201 gtatacggac gtgctggcca ggacggtcgg catactgccg ccggaaaatg gtgtgattga
     1261 acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc ccctcgaccg
     1321 tgagaagggg ctgttcacat caggaattca tgtgctcgat gagctgtccg tccgggcact
     1381 cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa gtgtcccccg
     1441 gacggctggt tacagcgatg cggtcagtgt tctggcacag gaccgtccgt cgctggccat
     1501 tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac tggtcatgat
     1561 ggcccgggaa caggggcggg aggtgcagat tatcgctgct gaccgtcgct cgcagatgaa
     1621 cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc agctgctgga
     1681 aggcatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggtgaaa aactctccct
     1741 gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg tcctgataac
     1801 cgacagcggg cagcgaaccg gtacaggcag tgcgctgatg gccatgaagg atgccggggt
     1861 gaacacatat cgctggcagg gcggagaaca gcgaccggcc accatcatca gtgaaccgga
     1921 ccgtaatgtc cgctatgccc ggctggccgg agattttgcg gccagcgtga aagccggaga
     1981 agagagcgtg gcacaggtca gcggggtacg ggaacaggcc atactgacac aggccattcg
     2041 cagtgagctg aaaacgcagg gcgtgctcgg acaccctgag gtgaccatga ctgcactgtc
     2101 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgac cggggatggt
     2161 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgttatcg accgggtgac
     2221 ggcgcagagt cacagcctga ccctgcggga tgcgcagggc gaaacgcagg tggtgcgtat
     2281 ttcctccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     2341 cgagcgactg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     2401 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccggggc gggctgaacc
     2461 ggccaccctg cctgtggctg attcaccgtt cacggcactg aagctggaga acggctgggt
     2521 ggaaacgccc gggcattccg tcagtgacag tgcgacggtg tttgcctccg tcacacagat
     2581 ggcaatggac aacgccaccc tgaacggtct ggcccgcagc ggtcgtgatg tccggctgta
     2641 ttcctcactg gatgaaaccc gtactgcgga aaaactcgcc cgccatccgt cctttacggt
     2701 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     2761 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     2821 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     2881 tgcagaagga accggtttta ctgaactggg aggggaaatc aatgcgcaga taaaacgcgg
     2941 tgacttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3001 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggcaagg aggcggtcac
     3061 gccgctgatg gagagagtac ccggcgaact catggagacg ttaacgtcgg gacagcgtgc
     3121 cgccacccgt atgatactgg aaacgtccga ccgtttcacg gtggtacagg ggtatgccgg
     3181 tgtgggtaag accactcagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3241 tgagcgtccc cgggttgtgg ggctggggcc cacgcaccgt gcggtcggtg agatgcgcag
     3301 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     3361 cagcggagaa acgccggatt tcagcaacac gctgtttctg ctcgatgaga gctcaatggt
     3421 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgtgctgt
     3481 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     3541 gcagacgcgc agtgctgccg atgtggccat catgaaggag attgtgcgtc agacgccgga
     3601 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcac tgtccgggct
     3661 tgagagtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     3721 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     3781 gctgaaaggc gaggctttcc cggatatccc catgacactg tatgaggcca ttgtccgcga
     3841 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     3901 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gtgagctggg
     3961 caaagagcag gtcatggtgc ctgtcctgaa cacagcgaat atacgtgacg gagaactgcg
     4021 tcgtctctcc acctgggaga aaaacccgga tgcccttgcc ctggtggata gtgtgtatca
     4081 ccggattgcc ggtatcagca aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4141 cacgcggctg atttcgcccc gggaggcggt ggctgaaggt gtcacactgt acaccccgga
     4201 caaaatccgg gtggggaccg gcgaccggat gcgcttcacg aagagtgacc gggagcgcgg
     4261 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacgctgtc
     4321 ggacggacag cagacccggg tgattcgccc cggtcaggag cgggcagagc aacatattga
     4381 cctggcctat gccatcactg cccacggtgc gcagggggcg agtgaaacct ttgccatcgc
     4441 gcttgaaggc acggaaggta accggaaaca gatggccggc tttgagtcag cctacgtggc
     4501 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     4561 tgccattaac aatgccgtac agaaaggaac tgcccacgat gtgctggagc cgaaaccgga
     4621 ccgggaggtc atgaatgcgc agcggctgtt cagtacggca cgggaactgc gggacgtggc
     4681 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc cggcacggtt
     4741 tattgcaccg gggcgcaaat atccgcagcc gtatgtggca ctgcctgctt ttgaccgtaa
     4801 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     4861 gggattcagc ggtgaaggac gggtgaaagg cagcggggat gcgcagtttg tggccctgca
     4921 gggcagccgt aacggcgaga gcctgctggc tgataatatg caggatggtg tccggattgc
     4981 ccgtgataac cccgacagcg gtgtggtggt gagaatcgcc ggtgaaggtc gtccgtggaa
     5041 tcccggtgcc atcaccggtg gtcgcgtgtg gggggatatc ccggacaaca gcgttcagcc
     5101 gggagccgga aatggcgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5161 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5221 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggagattg ccggacagga
     5281 gcgtgaccgg actgccactt ctgaacggga agccgcgctg ccggagagtg tgctgcgtga
     5341 atcacaacgg gagcgggaag cggtccggga ggttgcccgg gaaaatctgc tgcaggagcg
     5401 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccctgggcgg
     5461 agactgatac aggaagataa acgctgatga caacggataa cacgaacacg acacgtaacg
     5521 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     5581 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     5641 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctgtttg
     5701 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     5761 ggctgtgggg atgggggatt attgcccagt tcgcatatta tctggcaggc tttccctggt
     5821 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     5881 cgcgcagtgg gtggcgtaca gcagcggcca ttcttctgat ggccctgtgg gggcctttgt
     5941 ccggcaccag ttacggcatt gccgggctgc tgatgctggc agtcagccac cggctgtacc
     6001 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg tctgctggct gttattccgg
     6061 cgcttaacct tgccaccagt gatgcagcgg cggtggctgg tcttgtgatg acggtgctga
     6121 ccgttggtct ggtgtcgtgt gcaggaaaat cattaccccg tttctggccc ggggattttt
     6181 tcccggtatt ctacgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6241 ggtatctttg accgtaacag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6301 agcgaccggt actgacactg aagcgaaaaa cggaagggga gacgcctgtc cgcaggcgga
     6361 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     6421 agaaagcagc ccgggaagcg gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     6481 ccatttatct gaacctgccc tcgctggatg aggccgtgaa caccctgaaa ccctggtggc
     6541 cgggattatt tgacggtgaa acgccccggc ttctggcctg cggtatccgg gacgtgttac
     6601 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     6661 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     6721 atacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcggcagcgc
     6781 gtctggataa aatccgccgc cagaacagga taaaggcaga gcttcaggcc gtgcttgatg
     6841 agaaataaaa aaaagcctcc atcagagagg aggcagggaa ataaggctta aaaggaaata
     6901 acttccgtac ccgaaaggaa gccaattcag tagattattt aacaccataa tactgacggg
     6961 ttaaagagga gtgataaaga tgaatggatt cagaaacagt tcacggaacg gtcaggtctg
     7021 gcgctggcag cgtgccggaa gccgggccgt tattctggag gtcagtggac gctggatgga
     7081 agcggcagaa gcatggcgac gggctgccgg tgttgcgccc cggacagact ggcagcagtt
     7141 tgcccgaaaa agggctgaac actgccaccg gcgcagccgg ggaagggtgt aacgaaaaaa
     7201 gcagccttca ttactccagg gattcagaag gctgaataat g
//
LOCUS       DAZSBF010000211         7239 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZSBF010000211 DAZSBF010000000
VERSION     DAZSBF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.215"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..337
                     /locus_tag="ACHXWD_003498"
     CDS             <1..337
                     /locus_tag="ACHXWD_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7622313.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="ACHXWD_003499"
     CDS             337..666
                     /locus_tag="ACHXWD_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7622314.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="ACHXWD_003500"
     CDS             676..1374
                     /locus_tag="ACHXWD_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR7622315.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="ACHXWD_003501"
     CDS             1379..2122
                     /locus_tag="ACHXWD_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR7622316.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="ACHXWD_003502"
     CDS             2059..2661
                     /locus_tag="ACHXWD_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR7622317.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="ACHXWD_003503"
     CDS             2722..6201
                     /locus_tag="ACHXWD_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7622318.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="ACHXWD_003504"
     CDS             6269..6868
                     /locus_tag="ACHXWD_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR7622319.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="ACHXWD_003505"
     CDS             6932..>7239
                     /locus_tag="ACHXWD_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7622320.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cttttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgc
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAZSBF010000212         7163 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZSBF010000212 DAZSBF010000000
VERSION     DAZSBF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.216"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..4273)
                     /gene="traI"
                     /locus_tag="ACHXWD_003506"
     CDS             complement(<1..4273)
                     /gene="traI"
                     /locus_tag="ACHXWD_003506"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFR7622321.1"
                     /translation="MMSIAQVRSAGSADNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVTNVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKRKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     MYTDLLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLT
                     LRNAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDHFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     VGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLERVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRK"
     gene            4355..4582
                     /gene="vapB"
                     /locus_tag="ACHXWD_003507"
     CDS             4355..4582
                     /gene="vapB"
                     /locus_tag="ACHXWD_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFR7622322.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            4582..4980
                     /locus_tag="ACHXWD_003508"
     CDS             4582..4980
                     /locus_tag="ACHXWD_003508"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFR7622323.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(4989..>7163)
                     /gene="traD"
                     /locus_tag="ACHXWD_003509"
     CDS             complement(4989..>7163)
                     /gene="traD"
                     /locus_tag="ACHXWD_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFR7622324.1"
                     /translation="RIRMFSQIANIMLYCLFIFFWILVGLVLWVKISWQTFVNGCIYW
                     WCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMIWCGEQLWSAFVLATVVA
                     LVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVARMLKKDGRDSDIRIGDL
                     PIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVIYDRSGEFVKSYYDPSID
                     KILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDPFWQGSGRTIFAEAAYLM
                     RNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIEKTAISIRAVLTNYVKAI
                     RYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHASLKPVISMWLSIAIRGL
                     LAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCYVFGIQSYAQLEDIYGEK
                     AAATLFDVMNTRAFFRSPSHKIAEFAVGEIGEKEHLKASEQYSYGADPVRDGVSTGKD
                     MERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQERPKVAPEFIPRDINPEMEN
                     RLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQPQQPQQPQQPQQPQQPQ
                     QPQQPQQPVSPVINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDM
                     AAYEAWQQENHPDTWQQMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         1482 a         2207 c         1839 g         1635 t
ORIGIN      
        1 gtttccggtt accttccgtg ccttcaagcg cgatggcaaa ggtttcactc gccccctgtg
       61 caccgtgggc ggtgatggca taggccaggt caatatgttg ctctgcccgc tcctgaccgg
      121 ggcgaatcac acgggtctgc tgaccgtcag aaagtgtgac actgtcaccg gaaaccgctg
      181 tcaccgtcca gacgctgttg gccacataac cgcgctcccg gtcactcttc gtgaagcgta
      241 tcctgtcacc ggtccccacc cggatggtgt ccggggtgta cagcgtgacg ccttcagcca
      301 ccgcctcccg gggcgaaatc agccgcgtgt taccttccgc atcctgcagg gttatcagcc
      361 cgtcatcctt gctgataccg gcaatccggt gatacacatt atccaccagg gcaagggcat
      421 ccgggttatt ctcccaggtg gagagacgac gcagctcccc gtcgcgtata ttcgctgtgt
      481 tcaggacagg caccatgacc tgcacctgcc ccagttctcc ggccttttcc cgtgcatcat
      541 gaatcatgct gttcagtacg cgccgatcct cattcaggtg cgtgacaatc agcgtctgct
      601 cccgtgcttc cggtgtcctg ccggtatagt cgcggacaat ggcttcatac agtgtcatgg
      661 ggacatccgg aaaagcctcg cctttcagca tcgccttctg ctgcgcttct gccagtttcg
      721 cttcctggct gtgactgaac tccgtcacgg agtgctccgg tgcccatgcg ccctccagac
      781 gtggcacctg agacggtttc acacgctcaa gcccggacag cgccctttcc acatcccggt
      841 taatcaggct gtataccgcc tcccgcagtt ccggcgtctg acgcacaatc tccttcatga
      901 tgaccacatc ggcagcactg cgcgtctgct ggagacggaa aggctgacca ggcgcgatgg
      961 cctgcagctg gtccgtgtca ccgctggcca cagcccgacc gccaccgacc gcaatcaggg
     1021 cgtatgcccg tgccatgtcg gtattgccca ccattgagct ctcatcgagc aggaacagcg
     1081 tgttgctgaa atccggcgtt tctccgctgc gctgctgcag ctgcgtgtca tgcagaaagg
     1141 acgccagtgt ctgcgcatcc acgccggcgc tgcgcatctc cccgaccgcg cggtgcgtgg
     1201 gacccagccc aacgacacgg ggacgttcac tctccggcag catgttcacg gctgacatca
     1261 ccgcccggaa ctgtgtggtc ttacccacac cggcatagcc ctgcaccacc gtgaaatggt
     1321 cggacgtttc cagtatcata cgggtggcgg cgcgctgtcc tgatgtcagt ttctccatga
     1381 gttcgccagg tactctctcc atcagcggcg tgaccgcctc cttaccttcg agaatatggc
     1441 gaagaatgct cttttctgcc tcatacgacg cacgggaaac cagcaggcct gtgccatagc
     1501 cttttgccac atccacatac agtaaatcac cccgttttat ctgcgcattg atttcccctc
     1561 ccaggtcagc aaaaccggtt ccttctgcag caaacgactt cgcctctgtc agcaggtcca
     1621 ccatgctgaa ggccaggttt ttactttcca gcacaggaag ggccagatga atggcctgct
     1681 gtgccggcgt gtgaagcccg gctttctgca gactgatagc ggtttccagc aatgtctcac
     1741 cggcacgcgc ctttatctgc tcagaaacca ccgtaaagga gggatggcgg gcaagttttt
     1801 ccgcagtacg ggtttcatcc agtgaggaat acagccggac atcacgacca ctgcgggcca
     1861 gaccgttcag ggtggcattg tccattgcca tctgtgtgac ggaggcaaaa accgtcgcac
     1921 tgtcgctgac ggaatgcccg ggcgtttcca cccagccgtt ctccagcttc agtgccgtga
     1981 acggtgaatc gctcacaggc agggtggccg gttcagcccg tcccggcaca acaaccgtca
     2041 tcgcatcttc actgacggat gccacctgca ggcggtcacc gccggagacg cggagtccgg
     2101 gaattttccc tgtcaccctc agacgctcac cgtctgccac cggcattttt tccggccgga
     2161 acagcgacca gctgctgtcc agggaagaaa tacgcaccac ctgcgtttcg ccctgcgcat
     2221 tccgcagggt caggctgtga ctctgcgccg tcacacgttc tgtcacatag cggtcatgac
     2281 tgcgtgtctc cgggttccac tgctccatca ccattcccgg gcggtacata tcccgcagat
     2341 aacggctccg gctgtccagc cagaccggtg acagggcggt catggttacc tccgggtgtc
     2401 cgagcacgcc ctgtgttttc agctcactgc gaatggcctg tgtcagtatg gcctgttccc
     2461 gtactccgct gacctgtgcc acgctctctt ctccggcttt cacgctggcc gcaaaatctc
     2521 cggccagccg ggcatagcgg acattacggt ccggttcact gatgatggtg gccggtcgct
     2581 gttctccccc ctgccagcga tatgtgttca ccccggcatc cttcatggcc atcagtgcac
     2641 tgcctgtacc ggttcgctgc ccgctgtcgg ttatcaggac ctgtacgtta tgacgtgcgg
     2701 caccatccag cagggttaac gtctctttca gggagagttt ttcgccctgg tcgacgataa
     2761 cggtactgcc cggcgtgaag gccatgcctt ccagcaactg acgacgtccg gttatcagtt
     2821 caccggacag ccgttcatcc tgcttcaggt tcatctgcga gcgacggtca gcagcgataa
     2881 tctgcacctc ccgcccctgc tcccgggcca tcatgaccag ttcagccacc cgctcacgct
     2941 gcccggctgc accgccctgc ccggacacaa tggccagcga cggacggtcc tgtgccagaa
     3001 cactgacggc atcgctgtaa ccggccgtcc gggggacact tttctccgga tgtacggtca
     3061 cccggttctg tttcatgatg tcacgactga gtgcccggac tgacagctca tcgagcatat
     3121 gaatcccgga cgtgaacagc cccttctcac gatcgagggg gataagctgc tcacggctga
     3181 tggcctcatc gataccggcg cgtgcccgtt caatcacacc attttccggc ggcagtatgc
     3241 caaccgtcct ggccagcagg tccgtataca taaactgcac tttgcgttca cttaatccgg
     3301 caatggcctg tgtcaccgcc tgctgcacat ccggcccgtc ctgtgaagcg ggtccgggtg
     3361 tctgcgtgcg ggtatatgcc cgttgttccg ctgcgtcacg atatgcccgg atgtcgaacc
     3421 cggtttcctt cagcgtctgc atccactcgg ccattctgac ttcaggatcg acgtgctgtt
     3481 tggatttacg cgtatccagc gccgccacat cccgggattt cagcgaggcg tcttccccga
     3541 cggcctcccg gatagtctgt gagcgtccgg aaaaggcctc caccggtaca cccggcattt
     3601 cccacatacc gtgcttaccg accacttcag tttcatagcc cagcgcctca acctgctctt
     3661 tccgtttttc ccggtagagc ctgccaaagg caatctgatt agcgtacaca ttctcaatga
     3721 accccgtttt ccccactttg tcactgctca gtgtcttcca ttcgccgtta tgctgcgtaa
     3781 cattagtcac caccgcatgc gtgtgtaact gtggttcctg atcgcgactg gtgtcgtggt
     3841 taaacagtgc catcaccaga ttaccggtca gcaccgtttc tgactgtccg tccgtcatca
     3901 cccgtgtgga ggccagtgcc tccacctgac ggacagcaaa atccacggcc tggttatgtg
     3961 cttcaataag acgtttgtcc cccccgagca tggccatcat ggagacactt ttgggagcgg
     4021 agaaggtcag gtcgtagccg ggacgatgcc tgttactgcc atcctgcatg cggcttaaat
     4081 ccgccccgtc cggcagcctg ccctccagaa ggcgggtaaa gacatcctta tcgacactgc
     4141 cctgcagccc cagttgttca gccccctggc cggcccagcg ttctcccatg ctgcccagca
     4201 catagtaatt atccttgtcg gtataataat tatcggcact tccggccgat ctgacctgcg
     4261 cgatactcat cataaacgta tatccctttg acatatcccg gcatcaatcc cacaatagat
     4321 atacacaaga catatccaca taaggaggca aataatggaa accaccgtat ttctcagcaa
     4381 ccgcagccag gcggtcagac tgccaaaagc ggttgcattg ccggaaaacg taaaacgcgt
     4441 tgaagtgatt gctgtcggac gaaccagaat cattacgcca gccggagaga catgggacga
     4501 atggttcgac ggacacagcg tcagcgccga ttttatggat aacagggaac agcccggcat
     4561 gcaggaaagg gagtcattct gatgctgaag tttatgctcg ataccaacat ctgcattttt
     4621 acgataaaga acaaacccgc cagcgtcagg gagcgtttta acctgaacca ggggagaatg
     4681 tgtatcagtt cggtcaccct gatggagctg atatatggtg cagaaaaaag ccagatgcct
     4741 gaacgtaatc tcgctgtgat cgagggattt gtttcccgca ttgatgttct ggattacgac
     4801 gctgctgcag ccacacacac cggccagata agagcagaac ttgcccgtca gggacgccct
     4861 gtcgggccat ttgatcaaat gatcgcaggt catgcccgca gtcgggggct gattattgtg
     4921 actaataaca cccgggaatt tgaacgtgtg ggcggcctga gaactgaaga ctggagctga
     4981 cctgttcatc agaaatcatc tcccggctca acatcctccc cgcgttcccg gtgcacattg
     5041 atgttcacct cttcacgacg ctgcatctgc tgccaggtgt ccggatgatt ttcctgctgc
     5101 catgcctcat aagcggccat atccaccact tcaccggatt cactgatccc gggtggcagt
     5161 tgctgttcca tctcttcttc cggtttcatt ttcagctcct gctcaatccc ccctgccgga
     5221 acattcacac ctgaatctga cttcttatcg ttgatgacag gagacaccgg ctgttgcggc
     5281 tgttgcggct gttgcggctg ttgcggctgt tgcggctgtt gcggctgttg cggctgttgc
     5341 ggctgttgcg gctgttcagc ctgagtcacg tcttctccgg aaacaacctc cgggacatcc
     5401 ggttcgaaga ggctggccat ctgacgacct tctgcttccc ttgcggcaag tacggcactc
     5461 agacggttct ccatttccgg gttgatgtca cgcggaataa actccggagc gaccttcgga
     5521 cgttcctgat atttcagtga gagtttgact gccggatacg gtccgggcag ggtgacatag
     5581 caggtcagat ccggcaggga ctgaatgtcg gaataactga ccagcgtctg gcgctccata
     5641 tccttaccgg tcgatacccc gtcacgtacc ggatcagcac cgtaggaata ctgttcgctg
     5701 gctttcaggt gctctttctc accaatttca ccgacagcga actccgcaat cttatggctg
     5761 ggagaacgga aaaaggcacg ggtgttcatg acgtcaaaca gcgtggcagc cgctttctca
     5821 ccgtagatat cttccagctg ggcataggac tggataccaa acacataaca gccaccgaac
     5881 ttacgggctt ccggcaggat ctccaccagg tccggcagtt tgtgtaatgt gggtaactca
     5941 tcacagaaga accacacacg acgattacgg ttttctccca ttgccagcag accacgaatg
     6001 gcaatggaca gccacatgga gatcaccggc ttcagggagg catgagtgtc ggcattcgac
     6061 gagataaaca gccagccgtt tttctgatcc tcccggacac cacgcatcca gtcacggatg
     6121 gtgaagggct caccgttatg ctcaatcccc tgcaggtaac ggatggcttt cacatagttg
     6181 gtcagcacag cacggatgga aatcgccgtt ttctcaattt tctcttccac caggttggcc
     6241 gccggtgaat tacgcaggta ggtacgcagt ttttcaattt tgatggaaag cagtgtgtcc
     6301 accagtttgc tgtagctgcg gttggggtca ttacgcatca gatacgccgc ttccgcaaaa
     6361 atggtacgtc ctgaaccctg ccagaacggg tcctcttttg tgcccatcgg gatcagagta
     6421 tttgcggtat tatcaaaatc cggctgtgtc aggcactcct tccacaaatc ccaggcggca
     6481 cagcgtgcat ccagcggatt caggatctta tcgatggagg gatcatagta acttttaaca
     6541 aattcccctg aacggtcata aatcaccacc atatcaccac gctgacgggc gtagttggcc
     6601 agacgacgga taacctccga cttaccggcc ccgaccgtgc cgtgcagaca gaagttctgg
     6661 atttcggaat cccggataat cggcagatcg ccaatccgga tatcggagtc cctgccgtct
     6721 tttttcagca tccgggcaac gtctttcgga ttgtctgtca gttgacgacc acctgtgact
     6781 tcattttcgc tctgttgttt accctgacgc cccagaatcc aggagacaac aaagaaggta
     6841 atcaggcaaa taaccagtgc cacaactgtg gccagaacga atgcagacca taactgctca
     6901 ccgcaccaga tcatatattt atcatgcagt acctgagcag cgttcatccg gaaggttttg
     6961 ccgtaatact gaatctcata taccggctga gacttgatta aatcccgcat gccttccagc
     7021 gtggtacacc accagtaaat acagccattc acaaacgtct gccagcttat ttttacccat
     7081 aaaaccagac caacgagtat ccagaaaaaa ataaacaggc agtaaagcat aatattggcg
     7141 atctggctga acatgcggat acg
//
LOCUS       DAZSBF010000213         7121 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZSBF010000213 DAZSBF010000000
VERSION     DAZSBF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.217"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHXWD_003510"
     CDS             <1..435
                     /locus_tag="ACHXWD_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR7622325.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHXWD_003511"
     CDS             518..1420
                     /locus_tag="ACHXWD_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR7622326.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHXWD_003512"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHXWD_003512"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR7622327.1"
                     /translation="MDVSYLLDSLNDKQRETVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHXWD_003513"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHXWD_003513"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR7622328.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHXWD_003514"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHXWD_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR7622329.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHXWD_003515"
     CDS             complement(5330..6037)
                     /locus_tag="ACHXWD_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR7622330.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHXWD_003516"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHXWD_003516"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR7622331.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHXWD_003517"
     CDS             complement(6895..7098)
                     /locus_tag="ACHXWD_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR7622332.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1853 g         1638 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgtt tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZSBF010000214         7100 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZSBF010000214 DAZSBF010000000
VERSION     DAZSBF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.218"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHXWD_003518"
     CDS             <1..488
                     /locus_tag="ACHXWD_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR7622333.1"
                     /translation="TLLMRMNNLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
                     SLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAFSAILI
                     YALRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLD
                     K"
     gene            complement(558..1529)
                     /gene="rfaC"
                     /locus_tag="ACHXWD_003519"
     CDS             complement(558..1529)
                     /gene="rfaC"
                     /locus_tag="ACHXWD_003519"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR7622334.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(1533..2579)
                     /gene="rfaF"
                     /locus_tag="ACHXWD_003520"
     CDS             complement(1533..2579)
                     /gene="rfaF"
                     /locus_tag="ACHXWD_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR7622335.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2589..3521)
                     /gene="rfaD"
                     /locus_tag="ACHXWD_003521"
     CDS             complement(2589..3521)
                     /gene="rfaD"
                     /locus_tag="ACHXWD_003521"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR7622336.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3735..4931
                     /gene="kbl"
                     /locus_tag="ACHXWD_003522"
     CDS             3735..4931
                     /gene="kbl"
                     /locus_tag="ACHXWD_003522"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR7622337.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4941..5966
                     /gene="tdh"
                     /locus_tag="ACHXWD_003523"
     CDS             4941..5966
                     /gene="tdh"
                     /locus_tag="ACHXWD_003523"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR7622338.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6205..>7100
                     /gene="waaH"
                     /locus_tag="ACHXWD_003524"
     CDS             6205..>7100
                     /gene="waaH"
                     /locus_tag="ACHXWD_003524"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR7622339.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1742 a         1728 c         1789 g         1841 t
ORIGIN      
        1 atacattgct tatgagaatg aataacttaa ataacgattt agttaattat tcgcatgata
       61 acaccagaac ttcagtcggt gcccgtctgg caatgtatga agttggctta aaaacatatt
      121 ctccaatagg acaatcactg gaaaaacgag cggaaaaaat acatgaacta gaagaaaaag
      181 agcctagatt gagtggcgct ttaccctata tagattctca tttgcataac gatctcatag
      241 atacgttatc aacgcgtggt attcctggag ttgtattaac aattttagcc ttttcagcaa
      301 tactcatata tgccttaaga actgctaaag aaccttatat tttaatcttg cttttttcac
      361 tactggtagt aggcctaagt gatgtaatac tcttttctaa accggttccg actgctgtgt
      421 ttgtcaccat aatattgctt tgtgcttatt ttaaagcaca atcagaccaa tgtttattag
      481 ataaataatg aatatgtgaa ataaaatcag caaggaggct gattttattt caaaattatc
      541 tattaagggg ttgtaattta aatcatagca acgttttctt caatgaatcg cttaacagca
      601 tttgctgtca attgagacaa ctcattcccc ggagccctac aaaccatctg attcttccca
      661 tatccaccaa ttaatcccgg atcggttggt ccataaaccg tgatattggg tctatccagt
      721 gccgccgtta aatggcttaa ccccgtatcc accgacacta caaatttagc cccggccagc
      781 acgcgggcaa cgccttccag actcatcttc ggcaatactt caacataagc aaatccttcc
      841 gccagtcgtt tcgcccgctc ttcctcatgc ggcgcgcccc acggaagttt aatccgtatt
      901 cctgaatcag ccagtaaacc aatcaattct cgccagtgtt cttccggcca gtgtttatca
      961 tcacgggtcg tcgcatgaag aaatacggca tattcgccag catctgtagg caggttcgtc
     1021 aggaaatgct gtgcaatagc ataatcgccc tgggtttgcg gtttgctata gcccagactt
     1081 ttggcaaaca gttcgcgggt gcgttctacg gcgtgctgct gttttgcaat atgatgctta
     1141 cgattgtaaa acagactggc taaaggttcg cgagcggttt gccagtccat gccatgcttt
     1201 acgccatgcg ccagacgcgt caccagcgcc gcgcttttta ccagcccctg agcgtcgata
     1261 actgcgtcat agttctctgc ttgtagcgct tcacgaaacg ctttgcgttc cgcttttatg
     1321 ggggccgaga accaggcttt acgccagcga cgtattgcca caggaataac tcgctcaacg
     1381 gcggcgtgcc aggaaggaat ctgtgcgaac ccttcttcca ccacccagtc aaacttaatc
     1441 cctgggattg cctgctgggc atcagtgagt gcgggcaacg tatggagaac atcgcccatc
     1501 gacgatgttt taacgatcaa aacccgcatc cgtcaggctt cctcttgtaa caatagcgcg
     1561 ttgagttctt ccagtacgcg ctggggagta atgtcgatta agctctggtg ataaccctcc
     1621 gcagcgtcac ctttacgcac tttgtgatag ccggtaatca ggcggatcac gcgcgcttta
     1681 tgggatagcg gcggtgtgaa gtccgggcta ctcggaccat acagggcaac cagcggacga
     1741 ttgagcgccg ccgcaacgtg catcaggcca gaatcgttag tgacaatggc tttacaggct
     1801 gcaatcagga taaccgcttg atcaagctgt gtttcccccg ccaggttccg acaccatgcc
     1861 tgctgctcgg tattcaaagc ggcaagaatc tcattgcccg cttcatgatc tttcgccgag
     1921 ccaaacagaa ccacctgata accttcatca atcagctgct ttgccagttc cgcatagtgg
     1981 tagtgtggcc agcgttttgc cggaccaaac tccgcacccg ggcaaaaacc aatcatcgga
     2041 cgttctgatg aaagcgaaaa ttgattacag gtatatgatt tttcaccttc gctcacctgc
     2101 aattgcggcc ataacaacgg ttttggtaga tcttgtgcgg tacgcatcac gcctttgtca
     2161 taggccagcg cgacatagcg ttccaccatt agcggccagg cttctttatc gagcacgcgt
     2221 aaatcgttga gtaaaccgta gcgcatctcg ccgcgccagc cggtgcgatg aggaataccc
     2281 gcgaagaaag gcactaatgc agatttgaag gagttgggta agacgtaggc gcggtcgtag
     2341 cgcttttcac gcaggctatg acccagtttg cggcgttcgc cgatttccag cgctccgtga
     2401 ccgagaggca taggaatagc ttcgttaact tccggcatcc gcgataataa tggacggcac
     2461 catgccggtg ccatcacatc gattatcgcc tggggatagc gcgcctggag cgtgcgatag
     2521 agactttgcg acatcatcat gtcgccaacc caagacgggc cgatcaccag tattttcatg
     2581 cagagctctt atgcgtcgcg attcagccag gccatgtatt ccgttacacc ttcagcaacg
     2641 gttttgaacg gtttgtcgta acccgccgcg cgcagatttg tcagatctgc ctgagtgaac
     2701 gcctggtagc ggcctttcag cttatccggg aacggaatgt attcgatctg gcctttcttg
     2761 tgataagcca gcgtagcatc agctacagcc tggaaggatt ccgcacgacc agtaccgagg
     2821 ttgaagatgc cggaaacgcc attttccagg aaccacagat tcacatcagc cacgtcgccc
     2881 acatagacga agtcgcgttt gaagttctcg ctaccttcaa acagcttcgg tgattcaccg
     2941 ttgttaagct gggtgttgag atggaaagcg acgctcgcca tgctgccttt atggccttca
     3001 cgcggtccat aaacgttgaa atagcggaag ccaacaatct gcgagttagc ttcaggcagg
     3061 atttggcgaa catattcatc aaacaggaat tttgagtaac cgtagacgtt caacggtttt
     3121 tcgtactcgc gggattcaat aaagtcggag gtgcgtccgc cgtaggtggc tgcggaagag
     3181 gcatacagga acgggatttc gcgctccaga cagtagtgca gcagctcttt ggagtattga
     3241 tagttgttat ccatcatata cttgccatcc cactcggtgg tggaagagca cgcgccttcg
     3301 tggaaaatcg cttcgacatc gccgaactct tcgccagcca taatctggat caggaagtct
     3361 tccttatcca tatagtccgc gatatccaga tccaccaggt tcacaaactt ggtgccgtct
     3421 ttcaggttgt ccaccaccag aatatcggtg atgcctttat cattcagggc cttaacgatg
     3481 ttgctgccga taaagcccgc gccgccggta acgatgatca taactgtaac cttcgaatta
     3541 tggagtcaga gacaatctca gacacgaata cttctatcat atcactacac ggcgttggct
     3601 tcagccattc gccaaaagtg agattgatgg acgttattta tcctgaattt tgcagaagtg
     3661 ttaacgcgtc atctcgtcgc gacctataag tttgggtaat atgtgctgga atttgccctg
     3721 tctggagaat cgcaatgcgt ggagaatttt atcagcagtt aaccaacgat ctggaaaccg
     3781 cacgggcgga agggttgttt aaagaagagc gcattattac gtctgcgcag caagcagata
     3841 tcactgtggc tgatggaagc cacgtcatta acttttgtgc caataactat ctcgggctgg
     3901 cgaattatcc ggatctgatt gcggcggcaa aggcgggaat ggattctcac ggtttcggca
     3961 tggcttcggt gcgttttatt tgcggcactc aggacagcca taaagagctt gaacaaaaac
     4021 tggcggcatt cctggggatg gaagatgcga ttctctactc ttcctgcttt gatgctaacg
     4081 gtggcctgtt tgaaacgctg ctgggcgcgg aagacgccat tatctccgac gcactgaacc
     4141 acgcgtctat tattgatggt gtgcgtctgt gtaaagctaa acgctatcgc tatgccaaca
     4201 acgatatgca ggagctggaa gcacgtctga aagaagcgcg tgaagccggt gcgcgtcatg
     4261 tgctgattgc caccgatggt gtgttctcga tggacggcgt gattgccaac ctgaagggcg
     4321 tttgcgatct cgcagataaa tatgatgccc tggtgatggt agacgactcc cacgcggtcg
     4381 gttttgtcgg tgaaaatggt cgtggttccc atgaatactg cgatgtgatg ggccgggtcg
     4441 atattatcac cggcacgctc ggtaaagcgc tgggcggggc ttctggtggt tataccgcgg
     4501 cgcgcaaaga agtggttgag tggctgcgcc agcgttctcg tccgtacctg ttttccaact
     4561 cgctggcacc ggccattgtt gccgcatcca tcaaagtact ggagatggtc gaagcgggta
     4621 gcgagctgcg tgaccgtctg tgggcgaacg cgcgtcagtt ccgtgagcaa atgtcggcgg
     4681 cgggctttac cctggcggga gccgatcacg ccattattcc ggtcatgctt ggtgatgcgg
     4741 tagtggcgca gaaatttgcc cgtgagctgc aaaaagaggg catttacgtc accggtttct
     4801 tctatccggt cgttccgaaa ggtcaggcgc gtattcgtac ccagatgtct gcggcgcata
     4861 cccctgagca aattacgcgt gcagtagaag cattcacgcg tattggtaaa caactgggcg
     4921 ttattgcctg aggatgtgag atgaaagcgt tatccaaact gaaagcggaa gagggcatct
     4981 ggatgaccga cgttcctgta ccggaactcg ggcataacga tctgctgatt aaaatccgta
     5041 aaacagccat ctgcgggact gacgttcaca tctataactg ggatgagtgg tcgcaaaaaa
     5101 ccatcccggt gccgatggtc gtgggccatg aatatgtcgg tgaagtggta ggtattggtc
     5161 aggaagtgaa gggcttcaag attggcgatc gcgtttctgg cgaaggccat atcacctgcg
     5221 gtcactgtcg caactgtcgt ggcggtcgta cccatctgtg ccgcaacacg atcggcgtcg
     5281 gcgttaaccg cccgggctgc ttcgccgaat atctggtgat tccggcgttc aacgccttca
     5341 aaattcccga caatatctcc gacgacctgg cttccatttt tgatcccttc ggtaacgccg
     5401 tgcataccgc gctgtcgttc gatctggttg gcgaggatgt gctggtctct ggtgcaggcc
     5461 cgattggtat tatggcagcg gcggtggcga aacacgttgg tgcacgcaat gtggtgatca
     5521 ctgatgttaa cgaatatcgt ctcgaactgg cgcgcaaaat gggtatcacc cgtgcggtca
     5581 acgtcgctaa agaaaacctt aatgatgtga tggcagaact gggcatggcc gaaggctttg
     5641 atatcggtct ggaaatgtcc ggtgcgccgc cagcgtttcg taccatgctc gacaccatga
     5701 accacggcgg tcgcattgcg atgctgggta ttccaccgtc cgatatgtct atcgactgga
     5761 ccaaagtaat ctttaaaggc ttgttcatta aaggtattta cggtcgtgag atgtttgaaa
     5821 cctggtacaa gatggcggcg ctgattcagt caggtctgga tctctcgccg atcattaccc
     5881 atcgtttctc tatcgatgat ttccagaagg gctttgacgc tatgcgttcg ggccagtccg
     5941 ggaaagtaat tctgagctgg gattaacacg aacaagggct ggtattccag cccttttgcc
     6001 tgaggataat ccgttaaata tgtaaaatcc tgtcagtgta ataaagatat cgtaattgcg
     6061 ctgatctctt atatagctgc tctcattatc tctctaccct gaagtgactc tctcacctgt
     6121 aaaaataata tctcacaggc ttaatagttt cttaatacaa agcctgtaaa acgtcaggat
     6181 aacttcagag gtcgtcggta atttatgatg aacagcacca ataaacttag tgttattatt
     6241 ccgttatata atgcgggcga tgatttccgc acttgtatgg aatctttaat tacgcaaacc
     6301 tggactgctc tggaaatcat tattattaac gatggttcaa cggataattc tgttgaaata
     6361 gcaaagcatt acgcagaaaa ctatccgcac gttcgtttgt tgcatcaggc gaatgctggc
     6421 gcatcggtgg cgcgtaatcg tgggattgaa gtggcaacgg gcaaatatgt cgcttttgtc
     6481 gatgctgacg atgaagtcta tcccaccatg tacgaaacgc tgatgaccat ggcgttagag
     6541 gacgacctcg acgtggcgca gtgcaacgct gactggtgtt ttcgtgaaac gggagaaacc
     6601 tggaaatcca tccccagcga tcgccttcgc tcaaccggcg tattaaccgg cccggactgg
     6661 ctgcggatgg ggctttcttc gcgccgttgg actcacgttg tctggatggg ggtttatcgc
     6721 cgcgatgtta ttgttaaaaa taacattaaa tttattgccg gattacatca tcaggatatt
     6781 gtctggacaa cagaatttat gtttaatgcg ctgcgtgcgc gatataccga gcaatcgtta
     6841 tataaatatt atctgcataa tacgtcagtg agtcggttgc atagacaagg gaacaaaaac
     6901 cttaattatc aacgtcacta tattaagatt actcgtctgc tagagaaatt aaatcgaaat
     6961 tatgccgaca aaattacgat ttatccggaa tttcatcagc aaataactta cgaagcattg
     7021 cgtgtttgcc atgcggtgcg caaagagccg gatattatta cccgccaacg gatgattgcc
     7081 gagatattta cttccggtat
//
LOCUS       DAZSBF010000215         6987 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZSBF010000215 DAZSBF010000000
VERSION     DAZSBF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.219"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(203..625)
                     /locus_tag="ACHXWD_003525"
     CDS             complement(203..625)
                     /locus_tag="ACHXWD_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFR7622340.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TAFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            747..1025
                     /locus_tag="ACHXWD_003526"
     CDS             747..1025
                     /locus_tag="ACHXWD_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622341.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKIFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(1017..1307)
                     /locus_tag="ACHXWD_003527"
     CDS             complement(1017..1307)
                     /locus_tag="ACHXWD_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622342.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            1547..3193
                     /gene="cka"
                     /locus_tag="ACHXWD_003528"
     CDS             1547..3193
                     /gene="cka"
                     /locus_tag="ACHXWD_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFR7622343.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKL
                     NSYKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEVKAELA
                     KAESDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAV
                     ENELKNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEK
                     YAKIAQDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDEL
                     SKNLTKISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVAL
                     MFSFIVGVPLGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
     gene            complement(3190..3480)
                     /gene="cki"
                     /locus_tag="ACHXWD_003529"
     CDS             complement(3190..3480)
                     /gene="cki"
                     /locus_tag="ACHXWD_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFR7622344.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            3510..3641
                     /locus_tag="ACHXWD_003530"
     CDS             3510..3641
                     /locus_tag="ACHXWD_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR7622345.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(3836..3979)
                     /locus_tag="ACHXWD_003531"
     CDS             complement(3836..3979)
                     /locus_tag="ACHXWD_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622346.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDT
                     QNS"
     gene            complement(4646..4837)
                     /locus_tag="ACHXWD_003532"
     CDS             complement(4646..4837)
                     /locus_tag="ACHXWD_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR7622347.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            4905..5228
                     /locus_tag="ACHXWD_003533"
     CDS             4905..5228
                     /locus_tag="ACHXWD_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR7622348.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            5437..5871
                     /locus_tag="ACHXWD_003534"
                     /pseudo
     CDS             5437..5871
                     /locus_tag="ACHXWD_003534"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5906..6466
                     /locus_tag="ACHXWD_003535"
     CDS             5906..6466
                     /locus_tag="ACHXWD_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR7622349.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYLEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            6473..6706
                     /locus_tag="ACHXWD_003536"
     CDS             6473..6706
                     /locus_tag="ACHXWD_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR7622350.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
BASE COUNT         1877 a         1385 c         1941 g         1784 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgccattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagggatt atggtaaatc ccttactgtc tgccctcgta tccatcgaga taaaccgcag
      241 aaatggtgtc tgccattagt ttcatttttt tcggcgaagg catatcgctg tttgtgtatt
      301 ttctgccagc aaaggctgtt gtgctccgga gtgagtgaaa aaatttttct gagtggaatt
      361 cttttacacg ttgctgtgcc gtttctgctg atactccggc tgctttgtct ttcatcccca
      421 tatcccggaa ctgctgaaag aacgggagca tcatacgtat ggtgtctgct ttctgggtgt
      481 agaagttagg atcaaatcca tcatctgaag cataaacata atgccgggta tgtcgttcca
      541 ggctggtctt tggtgtgcat cctgtaagag ctattgttgc tgttatcaga ggtatcagaa
      601 gcagcctggc agcggcagat ctcataaaca tctccttgcg tttttaatct tgatactcag
      661 ggagttatat ctccgaagag tatctcttat catacgtcgg tttactccca atgtgatttg
      721 atggtcaatg agtcaggaat aatcccgtga aaaaatgtgt gccagtcctg ctttttagtg
      781 ttctttctgc ttcggttgcc agtcatgcgg acagcaatgt ttttcaacaa aaggccgaga
      841 agacaaaaaa cggcattgtt gcggatgtta atgccagaca gaaaatattc agtaacagac
      901 tgaagataca acttcagatc gatgagttga gagcagagct ggctggcacc agagatgccc
      961 aaaaaagaga ggatttgcgt aaaaaactgg agtctctgga gcggcaaaag aaatccttac
     1021 gctgatgggg gcgtattttc cccggtttct tccataacct ttttaccgtt cagatattta
     1081 cgggcgaggc gaatgcgtct tgcctggtat accgccgagt aagcctgggt gggattcaca
     1141 ccattactga tttcactgac cacatgacgc tgatcagcca gtgtcagcgt gtaaacctgt
     1201 ttctggatga ggtcacgggc tttgtcatgc tcatatccgg cagcaacaag ggtgtcgaga
     1261 agtcgtttgt ctgttttgtt cagtttatgg cggggtgatt tgctcacatc gttctccgtt
     1321 ttttcatgat ccaacagttt tatatctcat cctgatttgc tccggtgttc tgtttcgacg
     1381 ttctcaatgc ctgcctcccc atagtgtgat ccttattgca aaaatgaaaa tccatgctct
     1441 tgacatggac aatactgagt agtaggtttt actgtacata aaaccagtgg ttatatgtac
     1501 agtatattgt ttttaattta ttgttttaat tatcaaagag gaatttatgg ctaaagaact
     1561 aagtggatat ggaccaactg ctggtgagtc tatgggggga acaggggcga atctgaatca
     1621 gcaaggtgga aataataata gtaatagtgg ggttcactgg ggtggtggct ctgggcatgg
     1681 taataatggt ggtcagggaa attcaaattc atctggttct acatcaactg tgatgaaaac
     1741 cggtgagtca tatctgactc cgtggggtga tgttgtcatt aataatgatg gccttccggt
     1801 gatgaatggt attgttatga cagaagagaa ttcaactctg gttgataatc catttggagg
     1861 ggtctccaga gttcttaatt ctttaattag tgatatgcca tcactttttg cagaaagctc
     1921 aggaaataac aacaataaca ctgcttcagt taatactgca ccaacgaatg ctcaggtcag
     1981 tgatatggat aagagttcga aggtggtggg taatgtaatt aatgaaaaac aaaagcagaa
     2041 gaataaaatt gctacacaga ttagtgaaaa acagaagaaa atcgaggaga tgaaaaaagt
     2101 attcaagcat cattcttatc atggtatcac tgatcttgag agggatgttg atgagttgca
     2161 aaaaaaatca aatcagcttg atgcagatat tagcaaactt aattcttata aaaatacatt
     2221 gcaaagtaaa attggtgatg tgaataaaca gaaggaagct gaagaaaagg ctcgtgaaaa
     2281 tgcagaggtc gctgaacatg aaactcttaa tgaagagaaa caggcggtgg cggaagcaga
     2341 gaagcgttta gctgaggtga aagctgaact ggcgaaggct gaaagtgatg tacagagtaa
     2401 gcaagctact gtttccagag ttgcaggaga gcttgaaaac gctcagaaga gtgttgacgt
     2461 gaaggtcacc ggatttccgg gatggcgtga cgttcagaaa aaactacagc gtcagcttga
     2521 agctaagcag gctgagtact ccgcagttga aaatgaactt aagaatgctg ttagtttcag
     2581 ggatggtaag gccgctgagg ttaaagaggc agagcagaaa ttaaaagaag ctcaggatgc
     2641 tcttgaaaag agccagataa aagatgctgt tgatactatg gttggatttt atcaatatat
     2701 aaccgaacaa tatggagaaa aatacgcaaa aattgctcag gatttagcgg aaaagtctaa
     2761 aggaaagaaa attcaaggtg ttgatgaagc tctggcagca tttgaaaagt ataagaatgt
     2821 actggataag aaattcagta aggttgatcg ggatgctatt tttaatgcgt tagagtctgt
     2881 taattatgat gagttatcta agaatttaac gaagatatca aagtcgctta aaatcaccag
     2941 tcgagtatct tttctatatg atgttggttc tgattttaaa aatgcaatag aaactggtaa
     3001 ctggcgacct ttgtttgtca cgctggagaa aagtgcggtc gatgtaggtg tggcaaaaat
     3061 tgtggcatta atgtttagtt ttattgttgg tgtgcctctt ggtttctggg ggattgcaat
     3121 tgtcacaggt attgtttctt cttacatagg ggatgatgag ttgagcaaac ttaatgagtt
     3181 gttagggatt taatttctct ttagagtgta atatatgaaa cttggcaatc ctactgggat
     3241 tgccattaat aaataaaaca atgcagataa cttgcataag taccagggtt ctttttttaa
     3301 ctttgtattt tctttgatat atctagatat ggttaattta gagtatggat atagcaatac
     3361 atgtattgat gatataaata taaataacaa aggagtgtta tcattgtcgt taaatattaa
     3421 aataagaatc catggtataa gtgattcagg taaattatgt aggtagtatt ttaaatgcat
     3481 aagtattttt acttttgttg gaaagaaata tgaaaataat aatttttcta attacaatat
     3541 tattcttgtc aggatgtcaa gcaaactata ttcgtgatgt acaaggagga accgttgcac
     3601 catcttcgtc ttctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc
     3661 ttgagatcct ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac cgcctgggga
     3721 ggcggtttag tcgaaggtta agtcagttgg ggactgctta accgggtaac tggctttagt
     3781 ggagcacaga taccaaatgc tgtccttcca gtgtagccgc tataaggctt caacttcaag
     3841 agttctgcgt atctaaatcc actatccagt taccaatggc tgctgccagt ggcgttaagt
     3901 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3961 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4021 accaacagcg tgagctatga gaaagcgcca cgcttcccaa agggagaaag gcggacaggt
     4081 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagccacca gggggaaacg
     4141 cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg
     4201 atgctcgtca ggggggcgga gcctatggaa aaacgccagc tgcgtggcct tttccgggtt
     4261 cttgtttttt gctcacatgt ttcttttggt gttatcccct gattctgtgg ataacagcat
     4321 ttcgtttttg agtgagtaga taccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca
     4381 gtgagcgagg aagcggaaaa gcggctggac gtgcattttc tccttacgca tctgtgcggc
     4441 atttcacacc cggtatggcg cacttttcat accatccgca ctgatgccgc atggttaagc
     4501 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa
     4561 aacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggccagctg
     4621 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga
     4681 ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt
     4741 cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt ctgatgaagc
     4801 gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc catgtgtggg
     4861 gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc acgatacggg
     4921 tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat
     4981 ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg
     5041 caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag atagcccgga
     5101 agataaacag cggacagtgg tcggggcatg accgggttca tgtggtggct gcactgatgg
     5161 ccatcggacg tgaactgagt gagctgaggg atgaggtccg taaacaggga gaacgggatg
     5221 atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga ttacctgctg
     5281 ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga agaggtgcgg
     5341 gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct gtcttttgca
     5401 gagcagaccc tgccaccggg agagcgtgaa agggtgatgg agagctttga gcgggtactg
     5461 atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaagggg
     5521 cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa acggctccag
     5581 ccctattacg accgagcgga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac
     5641 cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt caccccgaat
     5701 aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt
     5761 taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt
     5821 tttgaggttg tgaggacaac caggagcagt atcagtatcg ccgacccgga gggtgggcgt
     5881 aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga cagacttcga
     5941 gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg agttcaggaa
     6001 gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca gagacgctat
     6061 cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc
     6121 gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc tctggaagct
     6181 ggtcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg gcgggatttt
     6241 tctggcgtca tgggggctga ttcagtggca ggaaagccgg attgcggcga acatcctggc
     6301 tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg gtgtgacgta
     6361 tctcgagggc aggaacggga aatttctggt gttgccgtcc ggggtgaagg gggaaaacaa
     6421 ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga gtatgacaga
     6481 gctggaaatg cacttactga acgccttaga gcaattgcag caggactata cgcagcggct
     6541 gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga cacgacagga
     6601 gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga gattagccgg
     6661 gcaggtggac cacttgagtc ggttatttat cacgaacagc agataaggga tgaacaggaa
     6721 aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa
     6781 gcacgggtat ggcgtggccc gtcaataggg atgtgacggt gagttgttcc gggaggttgc
     6841 cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt gtcgtttttg
     6901 ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc ccgcgatgtc
     6961 tgcgtatgtc atcaactgaa aagatgg
//
LOCUS       DAZSBF010000216         6905 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZSBF010000216 DAZSBF010000000
VERSION     DAZSBF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.220"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(308..877)
                     /locus_tag="ACHXWD_003537"
     CDS             complement(308..877)
                     /locus_tag="ACHXWD_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622351.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIVSHMLVTDTITE
                     ATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLFQGKM
                     STDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(936..4793)
                     /gene="sigA"
                     /locus_tag="ACHXWD_003538"
     CDS             complement(936..4793)
                     /gene="sigA"
                     /locus_tag="ACHXWD_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR7622352.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5154..6158)
                     /locus_tag="ACHXWD_003539"
                     /pseudo
     CDS             complement(5154..6158)
                     /locus_tag="ACHXWD_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6143..6520
                     /locus_tag="ACHXWD_003540"
                     /pseudo
     CDS             6143..6520
                     /locus_tag="ACHXWD_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6559..>6905)
                     /locus_tag="ACHXWD_003541"
     CDS             complement(6559..>6905)
                     /locus_tag="ACHXWD_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7622353.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1724 a         1531 c         1557 g         2093 t
ORIGIN      
        1 taccaatact ggcttaatgg ctacatacgg cagtcggttt acgcttacct ccgttaacgc
       61 ttacctccgt taacgcttac ctttaatctg ctgaatttcg aattgttcag gagtaatagt
      121 ccaaagtatc gggaattttc cagacaatcc tgataatacg cttatgaaca ttacaacacg
      181 ctcatgcgga acctggatgg gggaaggaac ggtgaagctg ttagcgaatg accggcgttt
      241 gtataaacgc cggtcatgat gaatgatatt tcagaatata cctcgggcta aaataccggg
      301 acagagatca ggcgtgatta atctgcagca tttttaataa cagaagtact gagcttactt
      361 tacctttgat gatgtaatag ttatttccgt cggtactcat ttttccctga aataatgtca
      421 cggatgtttc ttcctgctga tcctgcaggc gcagtcgtaa ggtcatattt ttaatgagct
      481 gagatccggg tggcaagggg tatttgctgt cagatactgg gcgactgagt ctgatgtggc
      541 gcgtatacag cccgttttcg tacagggtgg cctctgttat ggtgtcagtg accagcatgt
      601 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
      661 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      721 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      781 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      841 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      901 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      961 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
     1021 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
     1081 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
     1141 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
     1201 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
     1261 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
     1321 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
     1381 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
     1441 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
     1501 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
     1561 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1621 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1681 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1741 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1801 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1861 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1921 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1981 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     2041 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     2101 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     2161 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     2221 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     2281 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     2341 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     2401 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     2461 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     2521 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     2581 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2641 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2701 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2761 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2821 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2881 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2941 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     3001 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     3061 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     3121 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     3181 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     3241 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     3301 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     3361 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     3421 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     3481 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     3541 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3601 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3661 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3721 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3781 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3841 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3901 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3961 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     4021 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     4081 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     4141 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     4201 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     4261 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     4321 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     4381 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     4441 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     4501 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     4561 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4621 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4681 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4741 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4801 ctccgatatt tctgagttgt ggttatggga taacatttaa ttacaacggg gaactatcag
     4861 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4921 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4981 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     5041 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     5101 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     5161 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     5221 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     5281 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     5341 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     5401 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     5461 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     5521 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     5581 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5641 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5701 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5761 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5821 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5881 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5941 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     6001 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     6061 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     6121 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     6181 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     6241 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     6301 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     6361 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     6421 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     6481 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     6541 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6601 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6661 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6721 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6781 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6841 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6901 cacca
//
LOCUS       DAZSBF010000217         6877 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZSBF010000217 DAZSBF010000000
VERSION     DAZSBF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.221"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..366)
                     /gene="rnpB"
                     /locus_tag="ACHXWD_003542"
     ncRNA           complement(<1..366)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHXWD_003542"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(399..1544)
                     /gene="garK"
                     /locus_tag="ACHXWD_003543"
     CDS             complement(399..1544)
                     /gene="garK"
                     /locus_tag="ACHXWD_003543"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR7622354.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1641..2531)
                     /gene="garR"
                     /locus_tag="ACHXWD_003544"
     CDS             complement(1641..2531)
                     /gene="garR"
                     /locus_tag="ACHXWD_003544"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR7622355.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2561..3331)
                     /gene="garL"
                     /locus_tag="ACHXWD_003545"
     CDS             complement(2561..3331)
                     /gene="garL"
                     /locus_tag="ACHXWD_003545"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR7622356.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3347..4681)
                     /gene="garP"
                     /locus_tag="ACHXWD_003546"
     CDS             complement(3347..4681)
                     /gene="garP"
                     /locus_tag="ACHXWD_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR7622357.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5056..6627
                     /gene="garD"
                     /locus_tag="ACHXWD_003547"
     CDS             5056..6627
                     /gene="garD"
                     /locus_tag="ACHXWD_003547"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR7622358.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6776..>6877
                     /gene="prlF"
                     /locus_tag="ACHXWD_003548"
     CDS             6776..>6877
                     /gene="prlF"
                     /locus_tag="ACHXWD_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR7622359.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
BASE COUNT         1687 a         1887 c         1693 g         1610 t
ORIGIN      
        1 accgataagc cgggttctgt cgtggacagt cattcatcta ggccagcaat cgctcactgg
       61 ctcaagcagc ctacccgggt tcagtacggg ccgtacctta tgaaccccta tttggccttg
      121 ctccgggtgg agtttaccgt gccacggact gttaccagcc gcgcggtgcg ctcttaccgc
      181 accctttcac ccttacctga tcccgcttgc gcgggccatc ggcggtttgc tctctgttgc
      241 actggtcgtg ggtttccccc ccaggcgtta cctggcaccc tgccctatgg agcccggact
      301 ttcctcccct ccgcccgtct cccccgaaga ggacgacgac gaagcggcga ctgtctggtc
      361 agcttcggcg cgcagtatag agggtttgcg cgcccttgtc accccgcgtt gcgcattcca
      421 atcgccagtg tcgcggcgat attacgtgaa gcacggcaga tattgtcata agccccgcgg
      481 aatgcttcgt ccaacgtacc tatgctggtc aatacgctga agaccgcatc gatgccatgc
      541 tgatgtacaa cgccaacatc atcggtcagg ctacccgcaa tgccaatcac cggtttatgg
      601 tacttcttcg ccacgtttgc aacgccaatc ggtaccttgc cgtgaatact ctggctgtca
      661 atacgccctt caccggtgat caccagcgta caatcgtgaa tatgttcttc cagattcagc
      721 gccgtagtga cgatttcaat accacttttc agttccgcac caagaaacgc catcagcgcc
      781 gcgcccatac cgcccgcagc tcctgcgccg gggacatctt tcacatcaac atgcagcgct
      841 tttttaatga catcggcata gtgagagagg ttattgtcca gctcaacaat catcgcttca
      901 ctggctccct tttgtgggcc aaagatgcgc gatgcgccgt tatcgcccac cagcggattg
      961 gtgacatcac aagcgacgcg aatgacgcaa tcttttaagc gcggatcgag gccggaaata
     1021 tcaatatcat tcagagtatt aagactaccg ccgccaaaac caatttcatt gccgttggcg
     1081 tcgcataatt tcgcccccag cgcctgtacc atgcctgcgc cgccatcatt tgtagcgctg
     1141 ccgccaatgc cgataataat gtttgttgca ctgctctcca gcgcctgcag gattaactcg
     1201 cctgtgccgc gtgaagtggt tacgagtgga tcgcgttttt ccgcaggtac cagctccagc
     1261 ccactggccg ccgccatttc aataaacgcg gttttgccat cgccggagat cccccaactg
     1321 gcattcactt tctcgcccag cggccctgta acccaggcgt gacgttcagc cccctgggtg
     1381 gctgcaatca tcgcttccac cgttccttca ccaccgtcgg caaccggaac agaaacatac
     1441 tgtgcatcag gaaaaatttc ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca
     1501 gataaacttt ctttataaga gtctggggcg attacgattt tcatacctat gcctgttacc
     1561 acatgacgcc ggagggcgtt tctcttattc ggcctagatt ccaggcccgg attgcaatac
     1621 accatccggg cacgacgtca ttaacgagta acttcgactt tcgccagttt ttcgtagtag
     1681 cacgccaggg cgctatgatc cgccgttcct aaaccatctg cacgcagtgc ctgcatcatc
     1741 tccataaccg cagctgtgag cagcagttgt gcgccgacgc cgtgagaagt atccagcgca
     1801 ttcgccagat ccttaatatg cagatcaatg cggaagcccg gcttgaagtt gcggtccatc
     1861 accatcggcg ctttggcatc cagcacggta ctgcccgcca gcccaccgcg aattgcctga
     1921 taaaccaggt ccgggttaac gcccgctttg gttgccagcg ttaacgcttc tgacatcgca
     1981 gcaatattca gcgccacaat gacctgattt gccagtttgg tgacgttacc tgcaccgatt
     2041 tccccggtat gcaccacgga acccgccatc gctttcatca aatcatagta tttgtcgaaa
     2101 atagccttgt cgccgcccac catcactgac agcgtaccgt cgatggcttt cggttcaccg
     2161 ccgctcaccg gagcatccag catatcaatg cctttcgctt tcagcgcttc gctgatttca
     2221 cggcttgcca gcggtgcgat agaactcata tcgatcaata ccgtacctgg cttcgcgcct
     2281 tcaataatgc cattctcacc cagcgccacc tctttcacat gaggggagtt tggcagcatg
     2341 gttatgatga cgtcgcactg ttcagcgatc gctttagccg tagacgctgt ttctgcacct
     2401 gcagcaatca cgtcagcaat agcttctggg ttacggtcag caaccaccag cgagtaacct
     2461 gctttcagaa ggtttttact cattggttta cccataatcc ccaggccaat aaaaccaact
     2521 ttcatagtca tatcaaccat ctctcttgtt gcggtggtgg ttatttttta aaggtatcag
     2581 ccagtttctg agtggcagag cggaagacgc caagatcgct gccgacagcc acaaacgtcg
     2641 cgccccattc cagataacga cgcgcatcgg cttcgaccgg cgcgagaata ccgctgggtt
     2701 tgccgtgcgc gctggcacgg ttaaaaatat gctgaattgc tttttgtaca tccggatgcg
     2761 aggcattgcc gagatggccc aatgccgcag ccagatcgct ggggccgacg aagataccgt
     2821 ctacaccttc ggtagcggca atagcatcga cgttatctac gccctgctga ctttctatct
     2881 ggaccagaat agtaatgttc ttgttcgact gagcgaaata atccgccacg gtgccaaaca
     2941 tattggtgcg gtgagaaacg gagacgccgc gaatgccttc cggcgggtaa cgagttgatg
     3001 ccaccgcctg ctctgcttcc tcttttgttt ctacaaaagg aatcaggaag ttatagaaac
     3061 cgatatccag aagacgctta ataattaccg gctcgttggt cggcactcgc actactggcg
     3121 cgctggcgct gcctttcagg gccattaact gcggaataaa cgtggaaata tcgtttggcg
     3181 catgttcgcc atccagcacc agccagtcaa acccagccaa accaagaact tcagtgctaa
     3241 tcgggttaga gagtgctgac cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt
     3301 tgaatttatt cgggaaaaca tcgttattca tcgcttatac ctttgcttat ttctgcaatt
     3361 ccatacgttt aatgtcgcca actacgaaga ggtagcagac catcgccatc agcgctgaac
     3421 atcccacgaa aaccagtgct gcattgaagg agtgcagttc acttaccagg tagccaatca
     3481 ccagtggagt gacaatggag gcaacattgc caaagacgtt aaagacgccg ccacagaggc
     3541 caacaatctc tttcggcgcg gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt
     3601 tgccaaaaaa agccagcgcc atcagcatga ccaccagcgt ggtgttgttg gtgtagttac
     3661 ataagatgat ggtggaaacc agcaacattc ccagcacaat cggtagctta cgtgccaggg
     3721 tcagggataa accgcgtttg atcagataat ccgagaagac acctcccagc acgccgcccg
     3781 caaaaccaca cagtgccgga atcgaggcga ccagacccac tttcagaatc gacatgcctt
     3841 tttcctgcac cagataaatc gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa
     3901 aatattgtcc gaaaaatacg cccaacatca tgcggttaga gagcaattgc ttgatgtaat
     3961 gcagtttggg accgcttgct gccgcgctgc ccggcttttt gtggtccata tcgaccaccg
     4021 cgccattttc agagataaac ttcagctctt ccgcagacat acgtgggtga tctgtcgggt
     4081 tatgaatcaa cttgatccac agcgccgtca gcacaaaacc aatccccccc ataacggtga
     4141 agacgtgctc ccagccccaa gcgaaagtca gccagccaag cagcggcgaa aagagcgcca
     4201 gcgagaaata ttgcgccgag ttaaagatgg cggaggcagt accacgttct ttcgtcggga
     4261 accaggcggc gacaatacgg gcgttcgccg ggaatgatgg cgcttccgag aaccccagca
     4321 taaagcgcat aaagaacatg gagatccctg cccaggccag cgggaacata tcaacaaagc
     4381 cttgcaggaa ggtgaacagc gaccagaaaa agaggctgta ggtgtaaact tttttcgagc
     4441 caaacttatc aagcaaccag ccgccgggga tttgcatcag caagtaggcc cagccaaaag
     4501 cagagaagat gtaacccatc gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac
     4561 cagcaataga cagcgttgca cgatcggcgt agttaacggc ggtaacaata aaaataatca
     4621 gtaatattaa atagctggta tgcacgcctt tcttttttac gtcaacggtg tccagaatca
     4681 ttttatttac ctcgggtact tatgctgatt tttattatta tggggaaggt gttatttatg
     4741 agttttattt atgccgttac gacaatgaac tcgggaatta gtataagcag cgggagaata
     4801 ataatcattg tgcaaatgct aatttaatca gtactattta aatattattt tgagcatatg
     4861 cacataaggt tgcgcgctaa agcacagatt tgcgctttac cttaccgggc ggcactgcaa
     4921 tccctgaaat gattgacatt gatcacattt ctgcgtttaa actcctgaca ttcttatttc
     4981 acccaatgaa gtcatttatt tttaaatgag accagatcct cattttaata acccctggct
     5041 ggagaatatt gcacaatggc caacatcgaa atcagacaag aaacgccaac tgcgttttat
     5101 ataaaagttc acgacacaga taatgtggca attattgtta atgataatgg cctgaaagca
     5161 ggaacgcgtt ttccggatgg gctggaatta attgaacata ctccccaggg gcataaagtc
     5221 gcattgctgg acattccagc taatggtgaa attattcgtt atggcgaagt gattggttac
     5281 gccgtgcgcg caatcccacg cggaagctgg atcgacgaat caatggttgt actaccggaa
     5341 gcgccgccgt tacacacgct gccactggca accaaagtcc cggccccctt accgccgctg
     5401 gaaggataca cctttgaggg ctatcgcaat gccgatggca gcgtgggcac caaaaacctg
     5461 ctcggcatca ccaccagcgt ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc
     5521 attgaacgcg atctgctacc gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat
     5581 ttgtacggct gtggcgtggc gattaacgca ccggcggcag ttgtgcctat tcgtaccatc
     5641 cacaatattt cgctgaaccc taactttggc ggcgaagtaa tggtgattgg tctgggttgt
     5701 gaaaagttgc agcctgagcg cctgctggtc ggcacggatg atgtgcaagc tattccagta
     5761 gaaagcgcca gcattgtcag tttgcaggat gaaaagcatg tcggctttca gtccatggtc
     5821 gaggatattt tgcaggtcgc cgaacgccat ctacaaaaac tgaatcaacg gcagcgagaa
     5881 acctgcccgg cttcagaact ggtcgttggt gtgcagtgcg gtggcagcga tgcgttttct
     5941 ggtgtaacgg caaacccggc ggttggctat gcgtctgatc tactggtgcg ctgcggcgca
     6001 acggtgatgt tttcagaagt aacggaagtg cgtgacgcga tccatctgct gacaccacgc
     6061 gcagtgaacg aagaggtcgg caaacggctg ctggaggaga tggagtggta cgataactat
     6121 ctcaatatgg gaaaaaccga ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt
     6181 ctggcaaatg tggtggagaa ggcactcggc tccattgcta aatcgggtaa aagtgcaatt
     6241 gttgaagtgc tgtcgcccgg tcaacgcccg actaaacgcg gattaattta cgccgcgacg
     6301 ccagccagcg attttgtctg tggcacgcaa caggtggctt cgggtatcac cgtacaagtg
     6361 tttacgaccg gccgtggtac gccgtacggc ctgatggcgg tacccgtaat taaaatggca
     6421 acccgcaccg agctggcgaa ccgctggttt gatttaatgg atattaacgc gggcactatc
     6481 gctaccggcg aagagagcat tgaagaggtg ggctggaagt tgttccactt tattctcgac
     6541 gtcgccagcg ggaagaagaa aaccttctcg gatcaatggg ggctgcataa ccagctggcg
     6601 gtgtttaacc cggcaccggt gacctgattt cttttggaac gtccctcgca aaacatggcc
     6661 ttagtgccat gtttttattg tttaaagccc ccacgtccat taataatgca tttgcattac
     6721 cttagttcaa gcttataatt tgagcagaaa acaggctgta aaaggacaat gaatcatgcc
     6781 cgctaatgct cgctctcacg ctgtactgac cactgaatca aaggtcacga tacgcggaca
     6841 aacaactatc cccgcgccag tgcgtgaggc cttaaaa
//
LOCUS       DAZSBF010000218         6850 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZSBF010000218 DAZSBF010000000
VERSION     DAZSBF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.222"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..213)
                     /gene="cas6e"
                     /locus_tag="ACHXWD_003549"
     CDS             complement(<1..213)
                     /gene="cas6e"
                     /locus_tag="ACHXWD_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR7622360.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(138..884)
                     /gene="cas5e"
                     /locus_tag="ACHXWD_003550"
     CDS             complement(138..884)
                     /gene="cas5e"
                     /locus_tag="ACHXWD_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR7622361.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="ACHXWD_003551"
     CDS             complement(895..1950)
                     /gene="cas7e"
                     /locus_tag="ACHXWD_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR7622362.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1962..2498)
                     /gene="casB"
                     /locus_tag="ACHXWD_003552"
                     /gene_synonym="cse2"
     CDS             complement(1962..2498)
                     /gene="casB"
                     /locus_tag="ACHXWD_003552"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR7622363.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2495..4056)
                     /gene="casA"
                     /locus_tag="ACHXWD_003553"
                     /gene_synonym="cse1"
                     /pseudo
     CDS             complement(2495..4056)
                     /gene="casA"
                     /locus_tag="ACHXWD_003553"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            complement(4154..>6850)
                     /gene="cas3"
                     /locus_tag="ACHXWD_003554"
     CDS             complement(4154..>6850)
                     /gene="cas3"
                     /locus_tag="ACHXWD_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR7622364.1"
                     /translation="RKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDV
                     AAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPE
                     LKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTG
                     HHGWPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWY
                     ISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGIN
                     TLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQG
                     LFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVG
                     SEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKI
                     LLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQ
                     EAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQG
                     KCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKED
                     SSQRAGKVLICTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDG
                     KDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMP
                     HAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYL
                     PEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLE
                     GEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1661 a         1862 c         1621 g         1706 t
ORIGIN      
        1 gagttcttcc cgacgataaa gaaattgcct ttctttgccg ccagggaaaa gatcccacag
       61 ccactgatgc atcacatatt caccgcgatc caccagatgc agcaactgcg caggcgaaag
      121 ctggccggta tgtaaggtta ttcttgagag gtacatggtt cctccttgct gagccacggc
      181 ccctgattga cggtgcgttc cccaaacagc cactgctgac gatttaaagg aacatcccgg
      241 cggcgtaata ttttgctcgc gaccaggccg tcgtgtttcc cttcccacca gcattcatcc
      301 tgaagtttcg ggagtgagac tttcagttta tggaaatgat cctgatactg ttggtatgca
      361 ttacataagg catcacacgc gttgccttcg agcagtaacg gcgcaagagg taatgccagc
      421 ggatgacttt ttcgccccag ataaagcgga aaaactggat ggcgtaaacc gtcctgcaac
      481 tgttcgaggc tgtaaggcgc atcggctgtt gttgccaccg ccaccatcca ccaggcatcg
      541 gtgtagtagt cgcgccggga gataatcgcg ctcaaaagct cagggtcgct caactcttca
      601 cgacggctga aataacgtgc tttacgcacc tcttttggca tctggaccgt gtgataatcc
      661 cgtgcccagc gcgggttacg gctggcgcaa accaccagtg aatagtggcg attaaacgcg
      721 tttaagcgtt cggtatcatc acgccgaatc cctaccccgg cagccagtag ccccagcaat
      781 gctgagcgcg aaggaagttc atgggtatga cgaacttcac ctggagcatc gacaccccag
      841 gacgccatcg gcccatgaag ctgaaaaatc agatattggc tcataagccg ccccttacgc
      901 gcagataaag tccagcacgt ccttcatgct tccctgtttg ttcatcacgt caaaacttgc
      961 gcattcggtc ttctgttcgt agaccgtatt catgttttcg cgaagcgttg taatacgctg
     1021 caccgccaca tctaactgac gtgtgccatt aatgggttca tagaaagccg ccgccagaga
     1081 gcgtggttga tcggtgcctt tttctgccag cgcccaggaa gcataggcac ggctggcaaa
     1141 gctattttgt ttaccggttg gggaaacttt aagcgcggct tccgtaaaag cgcgtatggt
     1201 ctgattagct aacgcttcgt caccgccgag gttttctacc agcagatctt tatcaatgca
     1261 gatataggtg tagaaaagtg ctgaaccgaa tccggtttct ccgagatgcc cggcaccggc
     1321 atcttcagag gcctggcgca aatcatcaac ggcggtgaaa aaatcatctt cgacaatcgt
     1381 ttcactgaca ccaaacgcat gcgcgacctg gcaggcggct tcaacattaa actcaggttt
     1441 attcgccagc atacgaccaa acatagcgat atctaccgct atacggtctt tacgtaacaa
     1501 cgcgagatct tcctcttttg gcgcgcgctt ttcttctgcc agttgatgag caagcgcttt
     1561 tacggcgtca aattctgccg ggctgatatg aactaattgt tcagtttcgg cgttagtgag
     1621 cggatctttt ggttttttgt catttttagc tttcccaaga taatcagcaa tttttgccgc
     1681 ccattcgatg gcttttttat cttcaatccc tttctcaatc aggatagttg ccgcctcacg
     1741 cgcaatacgc ccactgcgaa taccaatatg gcccgccagt gcctgttcaa aaagtgcaga
     1801 agtgcgccac gcacgtttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag
     1861 caccacagtt tttggcgcac cggtgtcatc acggttaagg ttggcagcag ggtaagcggt
     1921 taacacatga agctgaataa atgttgtcat gagatagtcc tttattgatt ttgttcgtta
     1981 tcggcgtcgc cagcctggta atattccagc gcccagcgga tacggataaa ttcggttggc
     2041 cgctgctggc ggcggtgatg attgagcagg tcgtcgctct cctggcacca gcggaaaatg
     2101 ccatctgcca gggagtcaag attgacggaa ccgtttaata atctgacggc gcggcgtaac
     2161 tggcgcagta attcatccgg tgtttttact gctgacaggc gagtaaaacg cccctttgac
     2221 attaccgccg ccagttgcgc ggcaaaaggc tgccgttcat caatggcttt aacattggcg
     2281 ctaactgcgg ctatcagcgc cagtgcggtg atacgccact caggttcatc ctgccacttt
     2341 atttgtctgt tcttcagaaa caggcgaaat ccatccgtca gacaaacatc attaaccgtc
     2401 gtactacgcc gcagactggc acgttcgccg cgtttctcct gcaattcctc gtgccatttg
     2461 cgcagcgtgg ctttgtgctc ctcttttaca atactcattc agcagcctcc tgctttttct
     2521 ccctggcggc tttagcactt tgcttctccg ccgatgttgt aaaatatttc ttgcgcgcgg
     2581 tcatgatgcg ttccaaatca acgggctcat aaggattggt gaatacacgc tcgtcaaaat
     2641 cctgacgcgc gaataaccaa atttccttat tccatttgct gagtaattca tccgcatcct
     2701 gaccttcttc aatttggcgc actaacctca ggaagcgatg ctgagttttg ttccagaagt
     2761 cgatatccac aaaactaaaa tcaccccttg caccttttgg atcggagaac catgcttctt
     2821 tcaatgcact ccgtaacaga cttagaatcc gtgaagccgt ttgcgcagcc agccgcagtt
     2881 tcggtatctg gccttctttt tattgagcag cagcgggaaa tgatgttcgt accaacagcg
     2941 cgctttcatg ttgtcgaaat catagccaaa tccccacagg cccacttttg cctgcttgag
     3001 actgctggca ttaaagagtt tcaccaccag cgcgggaagt tccgtattgt tttctgactt
     3061 acccgtttcg ataaggccta accagtcgcg ccagattaaa ccgcccggtt gtggtttgac
     3121 cgagtaaaac tcgccgccct ctttaagtag tacacggtaa ggcgttaagg gatgctgcca
     3181 catggcatag ttagcgccgt aatttttggt cgtcatcagg ctcagaagcg cgtcgctctg
     3241 ctcaccgcaa atatcgcagt tgccgactgt ggtggtatta aaatcaatac gaatacggcg
     3301 aggcattccc cagtacgcct ggagtttatt gacctgctca tcggttacca ccgcaccggc
     3361 cagttcgctg atgcgcgtcg ggccaagcca ggggaaaacc agatcgtcaa attttttggg
     3421 tagcggtaaa tcggcttcat cctgcggcat gacattgatc cacagtttgc gccacaaggg
     3481 agtttgttga ttaccctgat attcctgtaa ttcaatcaga gtggtcatcg gcccaccgcc
     3541 gcgtaaaccg gtgcggtagc ctttgccacc tgacggcgca ttcaactgta gggagaacag
     3601 agctaacgca gagcaatgtg ggcacaggta ttccgttaca ccacgcttga taaagtggtc
     3661 tttattaaac ttcgttgttt gagcgccggg aatctcaggc agtagcgaag cgaccggaac
     3721 tttatctccg gtgagcgcct cgaaatcctg tataaatgaa ggggaatctg gcccaaactg
     3781 gaaagcatgt tctaatgaca gcaatgcttc ccgtagcttt tcagcttcca gtccgtcttc
     3841 ccagatatca tcccaacgac gatgatcttt tggcgcgaag ctggtttgta gtagccccag
     3901 caaaaactgc cataccgcac cctggagatc tgcccgcggc gcagaaatat cgacaacatt
     3961 ttcatccgcc agatcgactg gcgccagttt gcctgtcgtt ccgtctttaa aacgaacggg
     4021 caaccacggg gttgtcagaa gtgaaaacga gttcatacgc atcctctcgt aaaaaagacc
     4081 atcccaggtc ggttgtccat ctttccatcc ttttcggtgt atcaactatt cagagagtcc
     4141 tgtcgcagac atctcaacca accttgccaa tcaatccttc cgtagccgaa tacccacaac
     4201 tttcgtcatc gttgactaaa ataacgtttg ccatctccgg atcttgtcgc tgttcaatgc
     4261 accacagcct gaacgcctct ccctccagta aagaaaactc atcccgatgt tttttccacc
     4321 agcttcggcg cactctgaca acgctcattt cccatgcgtg agcaccggtg gcataaggct
     4381 tcaccacacc gtcaatacag gtagccagcc acagggaaac agactcctca gccagacgtg
     4441 tcgacagttt ttctgggagg taatcgttga tattggcggc atagccgggc ctgaagttca
     4501 gcacaaactt tttagccatt gcgcgatcac agtaatattt gcccacttgc tcctgctcgc
     4561 tgcgggcaaa tccttccggc attaccacgt cctcaccgta gacggattca ataagaagcc
     4621 gggctgcgtg tggcatctga atagcacctt gctcacgcag cacacgctgc gtcagccaga
     4681 ttcgcccatg atcgggatag acaaatgcac tgttacgcat ggcactgcca aaccattcgt
     4741 cgccaggaga gtcgtcccag acgggagcca gaatcagcaa ttcagggggg gaacgctcgt
     4801 cctttccgtc acgctttaac tgaccattaa tatcgcggat atgccgctgt aatcgtcctg
     4861 ctcgctgaat cagcaaatca acaggggcca ggtcggagat catttcgtcc aggtcacaat
     4921 cgacactctg ctctaatacc tgagtacaaa taaggacttt tccggcacgc tgtgagctat
     4981 cttctttacc aaagcgtgcc agtgtctccg tttcaattcg ctggcgatcg ctaaaagcaa
     5041 agcggctatg aaaaagtgaa aggctggaag caggaatgac gccgcgggca agcagctgac
     5101 gatgaacctt aatagcgtca tcgacagaat tccggatcca ggcgatgcat ttcccctgac
     5161 ttaccgccga ttcgatacgc gcaatacact cttgttcact atgaagccaa cccacgctga
     5221 cgctacgctc aacgtctttg cgcgtcgcta cccggtgtga gttcacatcg gatttcgtga
     5281 catgcgtcag ccaggggtaa tcatcctttt ccaggaacgg agcttcttgc aggccctctg
     5341 taccccgcgc aaaggcagcg acgagtttgt cgcgctgctg ttgagataac gtggcagaaa
     5401 gcaaaataac gctgtttccg ccacgcgcct gccgctcgat caacccttca agaatgcacg
     5461 acatgtaagc gtcacaggcg tggatctcat cagccagcag gattttgtta ctcagcccca
     5521 gaagccgcag attattatgt ttgaacggca tcaccgccat catcgcctga tccagcgtgc
     5581 cgacgccaat ttcagccagc aacgccttct tgttactgtc ggcaaaccag gccgcacatc
     5641 cctgactgaa cgtttgttca tccggttctt ctgacccgac taaatcacca gaccagagtg
     5701 attcattaaa gcggtccatt aatgtgcggg cactatgtgc cagcaccagg ctggggcggg
     5761 attctggcga atagaaagca agccaggttt tgaccagccg atcgtacatg gcattggccg
     5821 ttgccattgt tggcagacca aaaaacagcc cctgagcctt ccccgcagct atcaacctgt
     5881 gcgccaggat aagcgccgct tctgttttac ctgcgccagt cacatcttcc agaataaata
     5941 actgcggtcc ttgctggctg atatccagat ccagcacctt ttgctgtaac ggtgtcggat
     6001 tctcaataaa aggaaacagc gtattaattc cgttgaaagg cgcgactttt gcttttagag
     6061 gaaagacggt taaggcgttt tgagcctgaa ctaaagtttt ctgccagtaa cctttaatat
     6121 ccattgggtg tgcgacgcgt ggaaaaaatc gcgttgacga acccgtccag tctgcgagca
     6181 cgacggttgc agagatatac caggaaaggt gttttataag ttcaatgccc tcatcatcat
     6241 cccagaatgc ggggatctct atgagcggaa acagtggttt gatttcaagg agaaaatctc
     6301 gtgctgcagc tttgtcttca ggcagaaaat tatccaattc atccatacga tcagggggcc
     6361 aaccatgatg ccctgtcgtc acgggcatcc acatttctat cactcgccta agtttgcgcg
     6421 gagagagcga agatgaagga agtaactcct gacattcact taaataatga ttccacaacc
     6481 agtagcccag cgttgaatga gagatttttt cgtaattttt tctggcacct tcctgaatct
     6541 tgagttcagg gggcaggtaa agttgttgaa aagagcgggc aaattttcca atatcgtgcc
     6601 agcacagcaa ccaggcgaaa aattgagccg cctgttcctt gtcagaaatc cctaattgac
     6661 gaaaataatc agccagcccg aagcaatttc ttttaaccat taaatagccc atcgcggcta
     6721 catccagcga atgccagcaa agaaggtgat agtcgtcgcc cccgtctttc tcgccgcgtc
     6781 gggtttttcc ccagaaatca aaaaaagtca caatattttt atccttcagt aaacttaaag
     6841 gatatttacg
//
LOCUS       DAZSBF010000219         6779 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZSBF010000219 DAZSBF010000000
VERSION     DAZSBF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.223"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(228..713)
                     /gene="sixA"
                     /locus_tag="ACHXWD_003555"
     CDS             complement(228..713)
                     /gene="sixA"
                     /locus_tag="ACHXWD_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR7622365.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(916..3060)
                     /gene="fadJ"
                     /locus_tag="ACHXWD_003556"
     CDS             complement(916..3060)
                     /gene="fadJ"
                     /locus_tag="ACHXWD_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR7622366.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3060..4370)
                     /gene="fadI"
                     /locus_tag="ACHXWD_003557"
     CDS             complement(3060..4370)
                     /gene="fadI"
                     /locus_tag="ACHXWD_003557"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR7622367.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4550..4834)
                     /locus_tag="ACHXWD_003558"
     CDS             complement(4550..4834)
                     /locus_tag="ACHXWD_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR7622368.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5206..6546
                     /gene="fadL"
                     /locus_tag="ACHXWD_003559"
     CDS             5206..6546
                     /gene="fadL"
                     /locus_tag="ACHXWD_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR7622369.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1688 a         1817 c         1620 g         1654 t
ORIGIN      
        1 ctgtattaat aaatgacaat gagtgtcaaa cagacattat tcatttcaac atgtaacttt
       61 gggcaaaatc aagatacgcg cagaagaata atttccgaat gaaagtagaa agtgttttat
      121 ttatagatgg ggagagtctt cgatagtagt gagattgaca gatatccgct gcctgatgag
      181 caattatgcc caatgaatct acctcattgg gcataatttg gaactcatca gatagctttt
      241 gccatcttca gattacacgg gctcatctgc cagttgaatg tgccgtttcc gctttcatca
      301 agcgttacgc tggcaatggc cgaggtggta aacataggcg gcgtttcgcc cggacataac
      361 tcggcaacca gatacccgac taacggtaag tgagagatca ccagtaccga agcaacacct
      421 tcattggtca acgcctgcaa ataggcactg accagaccaa catcgccgca gggcgttaac
      481 tccggcagaa cttctgcact ggaaggcagg ttcagacaat cccctacttc ttccagtgtt
      541 tgctcggctc gcaggaacgg gctcaccaga acgcgttcga tttccacttt ttgacctttc
      601 agccagttcg ccatcaggcg agattcgtca caaccattag tggtcagagg acgaaccgaa
      661 tcactggcgg catcaagggc tgcgtcgccg tgacgcatga taaaaacttg catattgcac
      721 cgcttttgtt aaccagattc gtccgttttc tttcatctga aaccagagaa agaaaacggt
      781 ggccggcatt gtgccttatc cattcaccga atgaaacgct gttttttacc tcaatggcgt
      841 aagtatagtc aatatgcgtt tacattttga ccaacactcc gccattcagc gcggattcat
      901 atagctttga ccttcttatt gaaggtcagt tgcagttgtt ttccaaaaac tttccccacg
      961 cgcgcccatc tcgaccaaac gctcgcaagg ggtaaaacgg gaaccatact gcgtggccag
     1021 tcgttgcatt attgcaacca cttcgcccgc gccgagagaa tcgatatagc ggaacggtcc
     1081 accgagaaat ggcggaaaac caatgccaaa taccgcgcca atatccccgt cacgcacgct
     1141 acggataacc tgctcatcaa cacaacgtac tgcttcattc agcatcaaca tcacacaccg
     1201 ttcagcaacc tgcggtgcgg agagtcgccc ctgcccttgt gcgccaatga gcgggtaaat
     1261 ggcgggatcg acctgttttt tgcttttacg ccctttctga ccataaagat agaaaccccg
     1321 accatttttt ctgcctttgc gatcgtcgtt caaaattgaa gaaacaacat ttgcaggcgc
     1381 gctaaaacgt tctccataag cggcttccag tacaggaata attttagtcc cggtgtcgat
     1441 tcctacctca tccaaaagtt ggattgggcc taccggaaaa ccaaatttca ctagcgcggc
     1501 atcaatgtgc tctacccgtt caccttcggt cagcatgcgg atagcttcat taatgtaagg
     1561 cgctaagatg cgattgacgt aaaacccggc tttgtcacgc acgacaatgg gcgttttgcc
     1621 ctgttttttc gccagtttta ccgtagtggc gatggtttgc gccgatgtac ccgcatgagg
     1681 aataatctct accagcggca ttttttccac aggactgaag aaatgcaggc caataacttg
     1741 ctcaggtcgc gcggcgtgag cggcgatatc accaatcggt aaagatgacg tgttcgaagc
     1801 aaaaatggta tgagcggcgc aattttgctc cacttccgcc accatctgtt gttttaattc
     1861 gagattttca aacaccgctt caataatcag atcgcgatgg gcaaagccgc gatagtccgt
     1921 cgttccggag attaatgcca gctgtttgtc acgttcgctg gctttgagat gacgacggcg
     1981 aactttgccc tccagctgat cccaactgta cttcagcgca tgatttatgc cctgcgggtt
     2041 gatatcttta attctgaccg gaagccccgc tttacaagca gtgacataag caataccgcc
     2101 gcccatcaag ccaccaccta aaattcccac gctgtttaat ggcgcaggcg gtgcatcact
     2161 accgggatct ttcttcacgt ccgtactggc aaaaaagata ctacgcaaag cttgcgattg
     2221 cggcgtcatc gccagttcgc caaaggcgcg agcttcagcg tcatagccgc tgctggtgcc
     2281 ctgcgctaat cccgtttcaa caacctccag gatgcgttct gtcgccggat aattgccttg
     2341 agttttgtgt tctgttttct tgccgaccat tttgaacagc agcgcacgac ctaacggccc
     2401 cgccagaata cgctcgcgta caggtagagg gcgggaagat gggcgatcct tcttcgccag
     2461 ctcaacagcg gcttccagca aaatggagtg cggaacaacg tcatccacca gccccagctt
     2521 taatgcctgt ttcgcccgaa gttgttttcc ggtgagaatc atctctaatg ctgtactgac
     2581 gcctatcaga cgcggtaaac gctgggtgcc gcctgaaccg ggtaacaatc caagttgtac
     2641 ttcaggcaaa ccgagcaccg ttttaggatc gtcagtacaa acgcgaccgt ggcacgccag
     2701 cgccagctcc agcccaccgc ccaggcaagc gccatgaata gccgcgataa ccggaatggg
     2761 caaagcatga atctccgcca tcaactgttg gccctgccgc gccagtgctt ccgcttcttg
     2821 cgccgttttg cagttgccaa tcatgttgat gtctgctccg gcgataaaat tgtccggttt
     2881 cgcggagata aacaccacgc cgcgcaactc tttgttttca cggagttgct taataatggc
     2941 gcgcacctgc gaggcaaatt ccgccttcag ggtattcatt ttctcatccg gtacatcgat
     3001 ggtgatgata gcaatgttgt ccagacgaac attaagagta aacgctgatg ccatttccat
     3061 tattccgcct ccagaaccat tgccgcgcca agcccaccgg cagcacaggc ggtaactaaa
     3121 ccaaatccac cgccgcgacg gcgaagttca tgcaatgtct gggtaatcat ccgcgcgccg
     3181 gtcgccgcga agggatgccc gtaagcaatc gaaccgccaa gcacgttaaa tttgctatcg
     3241 tccacttcgc cagtggcatg tgcacgcccc agtacttcac gagcaaaacg ttcactaccc
     3301 agcaactgaa tattcgccag tgtctgagct gcaaaggctt cgtgcatatc gatcaatgtc
     3361 agttcgctca tcgtcaaacc ggcacgttcc agcgccagcg gtgttgacca ggctggaccg
     3421 agcaacatgt cctgccagac atcaatcgca gtaaatgcgt agctgcgcag ataccccagc
     3481 ggcaccagcc ctaattcttt cgcccgggat tcagtcatca ggatcaccgc tgccgcgcca
     3541 tcggtcagcg gcgtactgtt tgccgccgtt accgttccgt gtttgcgatc aaacgccggg
     3601 cgcagctttg cgtaatcggc aagcgaggaa ttaccgcgaa tattgttgtc ttcgacaagc
     3661 ggttgtttat aaggagggat aaaggcagtc atcacctctt ctttgagttt tccgtctgac
     3721 catgcctgag cggcacgctg atgcgaacgg tgcgctaatg catcttgctg ttctcgggtg
     3781 atgccgtagg ttttcgccat ttgttcggcg gtgtcgccca tccgcaagcc agtagaatat
     3841 tctgctaccg caggcggtac gggcattaag tcgcacaaac gcaggcgaga gaagagtttc
     3901 aggcgctggc tcatggttcg agctttgttg acatcaacca gcacgcgcgc cagtttttta
     3961 ctgacgccaa ttggcaatac ggaagaggaa tctgccccac cggcaatccc cgctcggata
     4021 gttcccgcca tcaggctttc tgcgacgttt gcaaccgcct ggaaactggt agcgcaagcg
     4081 cggctgacgc tgtaagcatc ggtatgcaca ttcattccgg taccgagaac aatttcacgc
     4141 gcaatgttgg gggcttcagg catttgtacg acctgaccaa agaccagctg ttcaatcact
     4201 tcagcgggga tctcgctgcg tgccagcagt tcgcctacca ccatcttccc taaatcaacc
     4261 gcgggaatgc catgaaaagc cgtcgcctgg cgggcaaaag gcgtacgtaa accgctaaca
     4321 atggcgatac gatcgccctg gcgggtaacc agcggtaaaa cctgacccat aacactcccc
     4381 tgtaaaaaaa taaataaagt ggtctgacct gatcatagtc ttaactattt ttttacattt
     4441 agccaagcgg agaaaaagga aagtgggagc tatgacacag tgaagacagg agaaagaaaa
     4501 gaaaacgccc ctgccagcaa ctgacagggg cgaacgtacc cgagaggaat taacgcagac
     4561 ccagctggaa gatcagcatt tcagcttcgc aggcaaaagt gaaatcgata tccaggcgca
     4621 caccgtcaga ctcttcagtg aaagtcgggg agattttgca cggttcggat tccacgctac
     4681 gggctttttc agtcagcgcc gccagcgttt gttctgcttc tgcgcggttt gcaaacacgc
     4741 ggctgtaaga cgcggtgcag tcggagttgt ccataatggt gccaacatcc atacagcagc
     4801 aaaccggggt ttcatcagca ctacatttac tcatcgttga tttcctctgt atgtgcaccc
     4861 aaggtgccag ataaacgttg tggatatttt acgcttccgg aaagtgctgc tccagttgtt
     4921 aattctgcaa aatcggataa gtgaccgaaa tcacacttaa aaatgatcta aaacaaaatt
     4981 cacccgaatc cgcgagtgcg caacctccaa attttgccag ctggatcgcg tttcttagat
     5041 catatttgaa aaaagatgga aacatacttg cagcattcca gctggtccga cctatactct
     5101 cgccactggt ctgatttcta agatgtacct cagaccctac acttcgcgct cctgttacag
     5161 cacgtaacat agtttgtata aaaataaatc attgaggtta tggtcatgag ccagaaaacc
     5221 ctgtttacaa agtctgctct cgcagtcgca gtggcactta tctccaccca ggcctggtcg
     5281 gcaggctttc agttaaacga attttcttcc tctggcctgg gccgggctta ttcaggggaa
     5341 ggcgcaattg ccgatgatgc aggtaacgtt agccgtaacc ccgcattgat taccatgttt
     5401 gaccgcccga cattttctgc gggtgcggtt tatattgacc cggatgtgaa tatcagcgga
     5461 acgtctccgt ctggtcgtag cctgaaagcc gataacatcg cgcctacggc atgggttccg
     5521 aacatgcact ttgttgcacc gattaacgac caatttggtt ggggcgcttc tattacctct
     5581 aactatggtc tggcaacaga gtttaatgat acttatgcag gcggctctgt cgggggtaca
     5641 actgaccttg aaaccatgaa cctgaactta agcggtgcgt atcgcttaaa taatgcatgg
     5701 agctttggtc ttggtttcaa cgccgtctac gctcgcgcga aaattgaacg tttcgcaggc
     5761 gatctggggc agttggttgc tggtcagatt atgcaatctc ctgccgggca aactcctcaa
     5821 gggcaagcat tggcagctac cgccaacggt atcgacagta ataccaaaat cgctcatctg
     5881 aacggtaacc agtggggctt tggctggaac gccggaatcc tgtatgaact ggataaaaat
     5941 aaccgctatg cactgaccta ccgttctgaa gtgaaaattg acttcaaagg taactacagc
     6001 agcgatctta atcgtgcgtt taataactac ggtttgccaa ttcctaccgc gacaggtggc
     6061 gcaacgcaat cgggttatct gacgctgaac ctgcctgaaa tgtgggaagt gtcaggttat
     6121 aaccgtgttg atccacagtg ggcgattcac tatagcctgg cttacaccag ctggagtcag
     6181 ttccagcagc tgaaagcgac ctcaaccagt ggcgacacgc tgttccagaa acatgaaggc
     6241 tttaaagatg cttaccgcat cgcgttgggt accacttatt actacgatga taactggacc
     6301 ttccgtaccg gtatcgcctt tgatgacagc ccagttccgg ctcagaatcg ttctatctcc
     6361 attccggacc aggaccgttt ctggctgagt gcaggtacga cttacgcatt taataaagat
     6421 gcttcagtcg acgttggtgt ttcttatatg cacggtcaga gcgtgaaaat taacgaaggc
     6481 ccataccagt tcgagtctga aggtaaagcc tggctgttcg gtactaactt taactacgcg
     6541 ttctgataac gcgttcgcct ggataaagtc acctgcatag caggtgactt taactccccc
     6601 acttcaccga aagtagtgtc ctcattgctt acctcctgag ttttgcaaac agcctgttgg
     6661 cagcttgccc tttcaaatca ataagcggta tccacagaca aacctgaagg gaaaggcata
     6721 ttttcaggcg ttctgctcgt ccttctcaaa gagtttactt ttctgcattt ccaggatac
//
LOCUS       DAZSBF010000220         6689 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZSBF010000220 DAZSBF010000000
VERSION     DAZSBF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.224"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..447
                     /locus_tag="ACHXWD_003560"
     CDS             <1..447
                     /locus_tag="ACHXWD_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7622370.1"
                     /translation="FKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVK
                     KEDKISDVIASSDHKNTQCPATDDELPDVDLDDEEDPEEELKREQRKAEIKERFRNAK
                     LKNKVSDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            447..743
                     /locus_tag="ACHXWD_003561"
     CDS             447..743
                     /locus_tag="ACHXWD_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622371.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            1067..1357
                     /locus_tag="ACHXWD_003562"
     CDS             1067..1357
                     /locus_tag="ACHXWD_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622372.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            1578..1793
                     /locus_tag="ACHXWD_003563"
     CDS             1578..1793
                     /locus_tag="ACHXWD_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622373.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            1940..2245
                     /locus_tag="ACHXWD_003564"
     CDS             1940..2245
                     /locus_tag="ACHXWD_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622374.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            2721..3431
                     /locus_tag="ACHXWD_003565"
     CDS             2721..3431
                     /locus_tag="ACHXWD_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7622375.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            3456..3662
                     /locus_tag="ACHXWD_003566"
     CDS             3456..3662
                     /locus_tag="ACHXWD_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622376.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            3761..5941
                     /locus_tag="ACHXWD_003567"
     CDS             3761..5941
                     /locus_tag="ACHXWD_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR7622377.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            6098..6689
                     /locus_tag="ACHXWD_003568"
                     /pseudo
     CDS             6098..6689
                     /locus_tag="ACHXWD_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640578.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
BASE COUNT         2201 a         1181 c         1463 g         1844 t
ORIGIN      
        1 tttaagaagg gagcttcgct ctgtgattca catgaatgtg attctcttgg tattgaagag
       61 gctattaaag agactgttaa aacatctgac ttagatgacc ttgatagtga agctgctaaa
      121 gaggtcgtta aaaaggaaga taaaatctct gatgtgattg ctagtagtga ccataaaaac
      181 acacagtgcc ccgccactga tgatgaatta ccggatgttg accttgatga tgaagaagac
      241 cctgaagagg aattgaaacg tgaacagcgt aaagcagaaa ttaaagaaag attcagaaat
      301 gcgaagctaa agaacaaggt tagtgacctc gatgactttg atgatagtga cgatgatttt
      361 acttttacac caaaaaagaa aactcacacc gagccaaaga aagaaaaagt taaagtcaga
      421 cgtgtttatg gtggaggaat gttttaatga aaacttataa tggcgagaga aaataccgtg
      481 actatttact cagacgttac agcatttctc gtgaaggtta cttgatgaaa gatactcacg
      541 gggaagttta ccggattcga ccgaagaaag aagggaggga ttattatttc gtcgatgatg
      601 tgacagattt aaagattgac gctttgaagt ttgcggtgct gtatcattac gatttatggg
      661 attcagtcca ccagctacga ttaaaagacg gtgaccccgg taatctgagg gccaccaata
      721 ttatcaaggg aaagtgccgg taaggccacc agctatttat ttactgaaat ctctatctca
      781 acacgaagaa gtttaagtcg aattgtaagc tttgtctttt ttgtaatgac tcgattcata
      841 acgcaaaaca ccaatagcac aataaatacc gcaaagccca acgagctaaa acgatgagat
      901 tcaataattg ctgtgaaaaa atcatgcatt aattttcctt ttgtgaaagg aaacagcacc
      961 tgtatcagcc cctacaacca tagtatcatg taaatgaggt actgacaata tgttaaatag
     1021 atgttaagat tgtagtatga gtaagagatt taaggagatt tttacaatga ctcacacagt
     1081 tacgcgcctt ttgccagagg catttgaacg actggcagca atcaaagcca aatatcccga
     1141 tatgaagcac acagcgcttg ttggggagat tttaaaagag tcggtaaacc ttagcccttt
     1201 cgataacgtt aagggcgtcg cagagggttc taaggccatc agaatcacaa cgggccaact
     1261 ggaactactc aaagcacagt ccgcagtagc aggttgctca atgattcagg tgttaagcgc
     1321 taaggtccat caactggcgg atagattgga ggtttaatgt gttataggtt ccaatattcc
     1381 acacttggcc gccacctaat cagacagaat ttagcagatg ctgaatttga agatgtgacc
     1441 ctcaaagcaa acaaacgatt aaaaccctct caaatccacc agcttaaaca cgaattaaag
     1501 ccactggtaa tactcgacta taacgaccct gaacagcttg agcagggaat taagcttatt
     1561 tctgaatggg gtgcttgatg aaaactatca cagtacaact tcagactaat aaagcatttc
     1621 gttattttga aaacttgctt gagttatacg aaggatgggg aagtatccac ggcaaagatg
     1681 atatctatct tcacttgtca gcccctaact attctctgaa gactccggtt aagcaaagct
     1741 ggttaaaaga ctacggtcac cagatggggc tattagtctc agacttgagc taaaaacagc
     1801 aaaacatatc tctcaggctt taccagacgc accagaaacg tttttaagcc cctaatcagg
     1861 gtaacttagg gaatcgcctt aagtggtcta aaaaaggggt taaaacgtct tagaatgcga
     1921 tacaggaagg attttaaaaa tgaattaccc aatgcaaatt atggttgacc aagaaaccat
     1981 gaattggttg attgacctga acaggtcggg ctttaatatc tatttcatgc attgttttga
     2041 agcgtcaacg tatattgaca agcatataat caacgaaatt aaacttggtg actatgttca
     2101 caagctggaa gtaacagacc catacaataa gcgctttatc attgaagagt gcaatcgact
     2161 tgatttaagc cctgagcaat tattgaagct gaatgtattc ttgacctatc agaatgaatt
     2221 aaacccttac agcgaggtga tgtaatgaca ctcgttattc tctggatgac tttagggtta
     2281 tttgttggtt gctgggtagg taatacggta aataagaatt gatgttacca atctgttaaa
     2341 aagctgttga caaataacct taagcatgag atgatggtaa tagggaagag caactcttcc
     2401 tgaattaaac tattaatcat tctacatcaa tgatagttat tctgaaaggt gcaatgcttt
     2461 tcgctacatg acctttcagg ttagtctgag aggtcatctc gaaaagtact cttgttcatt
     2521 cgtttgaatc ctttttgaat cctttagatg ttttaatttc ttcaccttat gaatggccct
     2581 ttataagaaa ttatctaggg ctattcagct tttaaaaagc atacttggca aaccgccttt
     2641 taagtctctt ctaagtttag ataggctgtc aggtgtgctt tttaaaaact gaaacacttc
     2701 cgcttagaag aggaaaagca atgacaaatg atatttacat taatggcaaa gtgattgata
     2761 gcttcagtga ctggtcgcca cagactgaac atccagctat cactattcag cgtaaagagc
     2821 atgacaagcg tattgagctt gagcaatcgc taaagatgtc accaaaaaca accatctttg
     2881 tgtctcctga gcctcaagag atgcctgata tttgcaagag tgaagcatta cttgagcttg
     2941 aaaagaaata ttaccaactg ctgaaagaac agcgccttag attagatgac gcttacagca
     3001 aagttacctt gatgcaatca gctattgagc cgtcagaatt tgagattcag gatgaattgt
     3061 cacagaaacc atttgtctat taccaatacg aagataatga tggttttggt acttttcaag
     3121 aaaacgtcgc agccgtgatt agtagtcttc ctgctggaca tcgaattgtg aagatggtta
     3181 aggcatcaag aggtgctggt cagtttattt acatgactaa taagagcgat gaagaactcg
     3241 ctgaaattgc tagacagaac atcttggcat cacgtaacaa gcaacttgat aatgttaagt
     3301 tgtatctctc aagagagctt caggctatga aagagcttgt gaaagaatat gaagatgcta
     3361 agaagattgc tatgcaggct gatattgagc agcttacgaa gatttctagc aaatatgcaa
     3421 aggctattta attaataatt aaaaggtggt ggtgaatgat tgatttaaca gaacgctatg
     3481 agcttattaa agcaattagt gaacgattga aagaacaacc caacaaacca aaacgaatca
     3541 aatcattcac ctcctctaag caaaagaata gaatcaggaa aatagctaag tggtgtcttg
     3601 ataaattacc aagtgattgt cagattgttg gtgaagatgg taattattat ctggttaggt
     3661 aacaagattg gttgggtgaa aatctacggg agagattatc gagtgatatc ggtagtcttt
     3721 ccctttttcg tatatgaaca cttaaagagg tgaaacatga atgagcaaat tcacagttga
     3781 tagttttatc gttgaactta gtttcagtga aaacgttatt aagggcttgc aaagagttga
     3841 aaaggcagct ttgcagtcag cacaaagaat tgaacgcaat ttgaatagag cgtttaaggt
     3901 cgataccaag caacttgaca gcaatctgac aagttcttta gggcagcttg aaaggaagtt
     3961 taataagacc tttgacaaga ttgagcagag agcaagaagc acaaaagcat ttcagtttgc
     4021 caccagattt aatgacactg ttaatcctcc aaaacaatca aggcaaccac gaattagcgg
     4081 aaacagagca atcacagcag cgcatagcgt gaatatgtct aagcttggtg atattaatcc
     4141 gctgatggct aaaatgttta aagcacagta ttacagctta agcggtaagg ctggtaatat
     4201 tggaagtgaa aagtttaacg ctgaacttgc aaaattaaat caatctcttc gtgaaacgtt
     4261 gaataaactc aggtcacaga caaagaccgc cagcattaac aatacaagcg acgcatttaa
     4321 taatcttgca agcaatgcga ttaaattatc tggtgcattt tactctgtga tgggtgctct
     4381 taacgcttac aaggccatta tgaatgcagg tcttaaacga gattccgcac agagagccgc
     4441 taagtttgta cttggtgata aagcatctga agctgaggta tttattcgaa atttagcaga
     4501 caagacaggt cttaatatct ctgaagggct atcaagctac gctaagtttg cagcaggtgc
     4561 tcagggttct atgtcacagg aacagacaca ggagctattt ggtaatgcaa cggctatgag
     4621 tcgcttaatg gggctttcta acgatgagct aaacggcatt ttgagagcat ttgagcagat
     4681 ggcgagcaag ggcaagattc aggctgagga attacgtggg cagttaggtg accgtatggc
     4741 tggtgcattt aagctattcg ctgaagcatt gggtatgact gctacagagc ttgatgcagc
     4801 catgaaaaac gggaaggtac tttccgctga tactctccct aaagtcgcta aacagatggg
     4861 cttgatgatt gataaagcgg gtggctgggc tgaagtcact aagagcactc agacagcttt
     4921 aggtaagtta gcaaacaatt gggatgatac gatggtgaaa atcttctcag gttcacagga
     4981 tgagctaaac ggcttcttat ccagcctgag taacctgctt agtgaaatgg ggatgagttc
     5041 ttctatcgct ggtgatgcta tcggtggact tatcgacatg cttaaagctg gtgtagatga
     5101 tattcgcgta ttcaataaca ccattgaagg ctggatttta cagactaaga agttctatta
     5161 cagccttgat gacactaaac gaaaactact tgatgaagtc ggtgatggtt ttatcaactt
     5221 cgtcaaaggt cttgctatag cgttatctgc aaagacgcta ttcagtgcta ctacaggggt
     5281 tatgaacctg acaagagcaa tcacaacgtt aggtacaaga gctaatcagg tggctggtca
     5341 ggtagctact ggtaaaggtg gtggaaaact gaaaggtgta gctggtggtg taggtagtgc
     5401 tcttgcaatt ggttatgctg atgacggtta tgaaacagcg ttagcattag cttcaatgat
     5461 tccacaaatc agaggcgtca ctcttggttt atatgcattg aagaaagcac tggattttat
     5521 gaatcaggaa gtagtcaaaa acgctaatat gcatccgtct ggggtaggtg tgggtagtga
     5581 ctttaatccc gtgtattctg gtgaccctaa taaaccgaat atgattaacg aaggatggag
     5641 tagaattttt agttcaatgg gtgaattgtt taacaatgcc acaatgaata ttgcaagatt
     5701 caatcatcca gagttaaaca acattaagca agattcagca ttaacgagtg cagatattca
     5761 gggtctgagg gatgaaatta gtgctttgag taagcgtatt caggagccag tgaaagtttc
     5821 acttggtggt caagttgtcg ttcgaccaga tgaaccatct ttctttactt tcaatcaatc
     5881 agctaatgac cagttcacgc aggatatgct cttaagttca agctatccag aggaagaatg
     5941 atttaacggt gttctacgtg aataaagcaa agttaatacc cacaccaaaa attatttttc
     6001 acgtagctca cgcttaacag agaaaataca tatatatcaa aggcttggtt aaaaatgatt
     6061 aaaatacact aagcctttga aatcaaagga gaatttaatg atttacacta atcagcttcg
     6121 ttacagtggc aaagatggca tctatttcca cttaagagac aatgtagatg ccttcttaac
     6181 cttatcagca actgaaaaca tggaatttga tagccctatg caggtgacta cacagaacat
     6241 gcaattatga aggaagctta ttattaactc gtcagggtca attagtagaa aacttcattg
     6301 acactctgga aaactggcgt gaccagaagc agattatttc tgttatctgt aaagatggaa
     6361 ttaaaattga cgattccatt attaccaact ttaaagcctc taaagatgtt actatctcta
     6421 acggtcttcg tatccagcta acttttcagg aaattaactt taaagcaatc gtaggtcaaa
     6481 ctgatatttc agcagccact ggcaaaacgg ctaccacaaa tgacggtggt gtgaccagta
     6541 agaagaatgc aggtaacgtg accactgaac gcaataacgc gcctatgcta tcgtgcaaag
     6601 agttaatgaa tattagcgat gtatccggtt acgctccagc tatcagaaat gatattctcg
     6661 atgctcagat taaatgtaat aaggcttgg
//
LOCUS       DAZSBF010000221         6627 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZSBF010000221 DAZSBF010000000
VERSION     DAZSBF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.225"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..487
                     /locus_tag="ACHXWD_003569"
     CDS             <1..487
                     /locus_tag="ACHXWD_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7622378.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            539..1474
                     /gene="ttcA"
                     /locus_tag="ACHXWD_003570"
     CDS             539..1474
                     /gene="ttcA"
                     /locus_tag="ACHXWD_003570"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR7622379.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHXWD_003571"
     CDS             complement(1603..2976)
                     /gene="dbpA"
                     /locus_tag="ACHXWD_003571"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR7622380.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHXWD_003572"
     CDS             complement(3006..3179)
                     /gene="ynaL"
                     /locus_tag="ACHXWD_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR7622381.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHXWD_003573"
     CDS             complement(3454..4437)
                     /gene="zntB"
                     /locus_tag="ACHXWD_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR7622382.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHXWD_003574"
     CDS             complement(4451..4498)
                     /gene="ydaN"
                     /locus_tag="ACHXWD_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR7622383.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHXWD_003575"
     CDS             4692..5924
                     /gene="dgcM"
                     /locus_tag="ACHXWD_003575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR7622384.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHXWD_003576"
     CDS             complement(5945..6508)
                     /gene="smrA"
                     /locus_tag="ACHXWD_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR7622385.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
BASE COUNT         1761 a         1695 c         1591 g         1580 t
ORIGIN      
        1 gaatgtcaga agaaacctga cgatacttga tatgttcggt cccccaaaaa caaatgccgg
       61 gatccggacg gtaacattac tgcagccggc tcttgaagca ctgaaggagc aatacaaact
      121 gaccgggcat catcgcaaaa gcgaaatcac tttttatcat cgggagtacg gcagaactga
      181 aaagcaaaaa ctgcattttg ttttcatgcc cagggtgtgt aacgaaaaac agaaacctta
      241 ttactcggta agcagtttgg gtgcaagatg gaatgcagca gtaaaacgtg ctggtattcg
      301 ccgccgtaat ccgtaccata cgcgacatac ttttgcctgc tggctgttga cggcaggagc
      361 gaacccggca tttatagcca gccagatggg gcatgaaact gcgcaaatgg tgtatgaaat
      421 ttacggtatg tggattgatg acatgaacga cgaacagata gccatgttga atgcgcggtt
      481 atcgtagttg caaagtttgc ccccaatttg ccccatttag taccagagaa ctgaaataat
      541 gcaagaaaat caacaaatta caaagaaaga acaatacaac ctgaacaaat tacaaaaacg
      601 tctgcgtcgt aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg
      661 catcatggtt tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa
      721 tttgcagcaa agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca
      781 accgggcttc ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa
      841 gattgtcgaa gagaacactt acggtatcgt gaaagagaag attccagagg gcaaaaccac
      901 ttgctcactg tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg
      961 ggcgacgaag atcgcgctgg gtcaccatcg tgacgatatc ctgcagacgc tgttcttaaa
     1021 tatgttctac ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa
     1081 acatatcgtt attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga
     1141 tgcaaaagcg ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg
     1201 tcaggtgatt gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac
     1261 gatgttcagc gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga
     1321 tttcaaaggc atcacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg
     1381 cgaagagatc ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga
     1441 tgagttacgg ctgaatgtgg ttgaagtgaa ataaccagga tggcgcccga tgcgcaagcg
     1501 tatcgggcta ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc
     1561 gaataagatg tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac
     1621 ccggctcgtt tttcctttaa tcttcccgcc ctgtaactgt ttccatgctt tatgagcaac
     1681 agcctgacgg accgcgacat agacatgcgc cggatgcacg gcgattttgc caatatctgc
     1741 gccatcaagc ccgatatctc ctgtcagtgc acctaataca tcacccgggc gcattttggc
     1801 ttttttccca ccatcgatac acaacgttgc catttctgct tccagcggca caatggaact
     1861 attagctggc ggcgtttgcc agttaagttt tatctgcaac atgtcagaaa tgatattggc
     1921 ccgctgtgct tcttccggag cacagaaact gatcgccaga ccgctatttc ctgcacgagc
     1981 tgtacggccg atgcgatgta catgaacttc agggtcccac gccagctcaa agttcaccac
     2041 cagctcaagc gatttaatat ccagaccacg cgcagcaaca tcagtcgcaa ccagtacacg
     2101 ggcgctaccg ttagcaaaac gtaccagggt ctgatcgcga tcgcgttgct ccaaatcgcc
     2161 gtgtaatgac aatgcacttt gccctacttc attcagcgcg tcgcagacag cctggcaatc
     2221 ttttttggta ttgcaaaaca ccacgcaaga ggatggctga tgcaagctta ataaccgttg
     2281 caacagagga attttgcctt tgctggatgt ctcataaaat tgttgttcaa tgggtggcaa
     2341 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattac
     2401 ggcaatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     2461 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     2521 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     2581 acgacccggc gttgccacga taatatgcgg cgcatgttgc aacgaatcac gctgcatacc
     2641 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     2701 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     2761 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     2821 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     2881 ggcggcctgc accggcgtca tagttaaata acccaactca ttaaggttcg tgagttgggc
     2941 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     3001 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     3061 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     3121 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     3181 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     3241 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     3301 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     3361 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     3421 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtgcaacca
     3481 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     3541 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     3601 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     3661 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     3721 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     3781 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     3841 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     3901 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     3961 gctggaatga tcggtcaacg catcgcacac atccaccagc caccctccgc aatcggtcgg
     4021 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     4081 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
     4141 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgagacggct
     4201 gactcggggc cgcgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
     4261 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
     4321 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
     4381 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccacagt
     4441 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
     4501 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
     4561 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
     4621 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
     4681 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
     4741 ggatcgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
     4801 tgcaagacct cacgtttagt cagctacgaa ccggtcccta ttccgtctcc tcacaaaaag
     4861 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
     4921 ttcagcgtaa agaagaggaa acagcattaa actgtcggct tgttttgcga aagctgacag
     4981 aagcagaacc ggtgattatt ttcgaaggta tcgaagcgtc ggcaacgctg ggtttaaaag
     5041 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
     5101 ctaactctgc accaatgttg ttgattgacc cctcacgaga tggccaaatc gtcgatgcta
     5161 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
     5221 gggaaataaa tatgctcggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
     5281 ccggtggtca taaacctctg aattttattc ataagctggc ggatggttcg actcgtcatg
     5341 tgcagaccta cgccgggccg attgaaattt atggcgacaa gctcatgtta tgtattgtac
     5401 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgcc caccatgacg
     5461 cgatgaccgg attactgaat cggcgacagt tttatcacat taccgagcca ggccaaatgc
     5521 agcatctcgc catcgcccag gattacagct tgttgctcat cgacaccgat cgttttaaac
     5581 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgca
     5641 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
     5701 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
     5761 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
     5821 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
     5881 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
     5941 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
     6001 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgca gtgcaatatg
     6061 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
     6121 gcgatttatc atcccgtcct ttgccatgaa taatcagcac gttacgcaaa ccatcagcca
     6181 tggcttgttg aatgaaactg aacatcattt tgcggcactc ttccaccggc tggcgcaaaa
     6241 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgtt
     6301 gcaacccttc ccgccgaaac tccagcggct gacttagtgg gatgatgtcg agaaatccgg
     6361 tggtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
     6421 ttggatgcca gtggacatcg gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
     6481 gaaacagcga tttgtcgtca aggttcatgt tacatcctcc cgcaattaag agcgcgatat
     6541 gataaccaga ccgggtcggt ccaacaacgt attacccaaa attccagtaa taagttccaa
     6601 atattgccga tattttaagc aaaatgc
//
LOCUS       DAZSBF010000222         6537 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZSBF010000222 DAZSBF010000000
VERSION     DAZSBF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.226"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="ACHXWD_003577"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="ACHXWD_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR7622386.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHXWD_003578"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="ACHXWD_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR7622387.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="ACHXWD_003579"
     CDS             2408..3157
                     /locus_tag="ACHXWD_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR7622388.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="ACHXWD_003580"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="ACHXWD_003580"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHXWD_003581"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="ACHXWD_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR7622389.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="ACHXWD_003582"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="ACHXWD_003582"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR7622390.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="ACHXWD_003583"
     CDS             6018..6491
                     /locus_tag="ACHXWD_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR7622391.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1650 c         1652 g         1628 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgtta
//
LOCUS       DAZSBF010000223         6514 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZSBF010000223 DAZSBF010000000
VERSION     DAZSBF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.227"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..184)
                     /locus_tag="ACHXWD_003584"
     CDS             complement(<1..184)
                     /locus_tag="ACHXWD_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7622392.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQE"
     gene            complement(785..1033)
                     /locus_tag="ACHXWD_003585"
     CDS             complement(785..1033)
                     /locus_tag="ACHXWD_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR7622393.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1296..1391)
                     /locus_tag="ACHXWD_003586"
                     /pseudo
     CDS             complement(1296..1391)
                     /locus_tag="ACHXWD_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1954)
                     /locus_tag="ACHXWD_003587"
     CDS             complement(1388..1954)
                     /locus_tag="ACHXWD_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR7622394.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2264..4036
                     /gene="aspS"
                     /locus_tag="ACHXWD_003588"
     CDS             2264..4036
                     /gene="aspS"
                     /locus_tag="ACHXWD_003588"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR7622395.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4154..4606
                     /gene="nudB"
                     /locus_tag="ACHXWD_003589"
     CDS             4154..4606
                     /gene="nudB"
                     /locus_tag="ACHXWD_003589"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR7622396.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4635..5375
                     /locus_tag="ACHXWD_003590"
     CDS             4635..5375
                     /locus_tag="ACHXWD_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7622397.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5410..5931
                     /gene="ruvC"
                     /locus_tag="ACHXWD_003591"
     CDS             5410..5931
                     /gene="ruvC"
                     /locus_tag="ACHXWD_003591"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR7622398.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(5933..>6514)
                     /locus_tag="ACHXWD_003592"
     CDS             complement(5933..>6514)
                     /locus_tag="ACHXWD_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR7622399.1"
                     /translation="EYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGHNGEDFLV
                     AESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLRKLYHTGF
                     KYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFLGSIPWER
                     KTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1627 a         1512 c         1724 g         1651 t
ORIGIN      
        1 tctcttggtt ttgtagacat gatactagtc gctgaaaagc gatgtctcgt tgttcttctt
       61 gtatgcggtt atttttccat tcagtccaat gggttagata attttcatat gcacggctag
      121 tgtcgattga ataagtggag aacgaatttg aaattaatct gtgattgtta tttgtcggga
      181 gcataaatat cagggcgtat tctttatgtt gagaggtgtt ggattctttt ttatctgctt
      241 tgcaatgtta ttggttccct tcagcagtag acaggaactt ctggaggagg cgaatatata
      301 gtttgtgtaa gcatattaac tccatatgtt atatattgaa gaacttctgc tttacactat
      361 cctaaactgg atcggtttaa tgtaaaaaaa cgatactttt tgggggaggg ggcaattttt
      421 ccttaaggta tgaaaaaagg agctgaagct atataatagc tattaatgcc actggtatca
      481 gtgaatcatg tatataaaaa actgtgttaa ggaggctgtc cattagcggt tttttattct
      541 tctggaaccg cggcatcttt gcgtatcttt ttgtcttctc accgtctggt cggtgttttg
      601 ctgagactgc taatttcctg tctttgctag tgctgtactg gtactgccca atcatgattg
      661 gcgggggcgg gcggagttga gaccgcagac acgtcgtatg caagaacgtg ctgcggctgg
      721 ctggtgaact tccgatagtg cgagtattga atgatttcca gccgttactg attttacgtg
      781 ctaattagtg aacaaaccac tcgtcagcag actcccaggt atctttcaga gtttcctgaa
      841 caaaagtttt agctgaatct ttatcggcgg tgcgcgtaac agaaaggcca tcgttgctgg
      901 tggcttttac gatcacctct acatcgtcat aacgtttact gatgcgtcgg gttaattctt
      961 cctttaacgc atccacagca ccggttggca ttttagtcat tttctctttg gctatgcaga
     1021 tttcaatacg cataaaaatc cctctacact gtgtttttat acagtattat ttttaactgt
     1081 atggataaac agtgtcaaga gtgagggaaa gtgatttttt ggggcatgca tggggcaaaa
     1141 atatttgttg tggggcattt tggggcatga ttgggacact ttgttacata tgaactttgc
     1201 cgattttcat atggcatggc aatccaacgc tttgattatt ctaaagaaaa cacatgctct
     1261 tgggcgttct ttagtgattt ttaaaaatcc cgtatcacgc agttaaagtg gcgggcatac
     1321 tcttcaaggc tggtgatccc caaccgcacc catttaggat gcgaccattg cggtagaccg
     1381 atgtaaatca taacgcgtga aggatctctt ccacgctacg cacacgggcg atgcgcgggt
     1441 agatatgatt aatgctgtta ttgtgttgct cggcgctggc ggcactacag gcgtcttcgg
     1501 caatcaccag attaaagcca agttcccagg cattgcgggc ggtggattca acaccgatat
     1561 tggtcgagat cccacataac actattgtat cgatacctcg gcggcgcagt tgcaactcca
     1621 gatccgtacc gtaaaacgca ccccactgac gtttgatgat ttcgatatca ctgtcagttg
     1681 cacctaatgc agctggatgt tgccaccagt tttcgggcaa cactttagcg ggggaggggg
     1741 catcaaccgg ctgttttaat gcttcggcgt aatcggcaga ccagccaact cgcaccagaa
     1801 acacgggctg accgctggcg cgaaatttcg ccgccagctt cccggcgcga ttaactacct
     1861 catcggcagt atgtggacca ccggcaaaag gtaagatgcc ttcttgtaaa tcgatcacca
     1921 ccaacgcagt ggttttagca ttaagttcaa gcatgctgac tcctgaaaaa acaaaatcgt
     1981 gcctcacacc ttaatgcgat tagccgatgg cggaacgaca aattttgtta atttttatga
     2041 gttcgggcaa cacctttata gcaaactggc gtagagtctg atttcaggcc cgatataaag
     2101 tggtaacgag gcgttacagc gcgggccagg ctcttacggt gcggcagaat ttccagtata
     2161 atagccgcct tttttcattc agttgtgaca tacagctaac gctgcgactt ggtcacctgc
     2221 gcagcagggg gcatccgccg gcagctaagt taagggatat ctcatgcgta cagaatattg
     2281 tggacagctc cgtttgtccc acgtggggca gcaggtgact ctgtgtggtt gggtcaaccg
     2341 tcgtcgtgat cttggtagcc tgatcttcat cgatatgcgc gaccgcgaag gtatcgtgca
     2401 ggtatttttc gatccggatc gtgcggacgc gttaaagctg gcctctgaac tgcgtaatga
     2461 gttctgcatt caggttacgg gcaccgtacg tgcgcgtgac gaaaaaaata ttaaccgcga
     2521 tatggcgacc ggcgaaattg aagtgctggc gtcctcgctg actatcatca accgcgcaga
     2581 tgttctgccg cttgactcta accacgtcaa caccgaagaa gcgcgtctga aataccgcta
     2641 cctcgacctg cgtcgcccgg aaatggcgca gcgcctgaaa acccgcgcta aaatcaccag
     2701 cctggtgcgc cgttttatgg atgaccacgg cttcctcgac atcgaaactc cgatgctgac
     2761 caaagccacg ccggaaggcg cgcgtgacta cttggtgcct tctcgtgtgc acaaaggtaa
     2821 attctacgca ctgccgcaat ccccacagtt gttcaaacag ctgctgatga tgtccggttt
     2881 tgaccgttac tatcagatcg ttaaatgctt ccgtgacgaa gacctgcgcg ctgatcgtca
     2941 gcctgaattt actcagatcg atgtggaaac ttctttcatg accgcgccgc aagtgcgtga
     3001 agtgatggaa gcgctggtgc gtcatttgtg gctggaagtg aagggcgtgg atctgggcga
     3061 tttcccggta atgacctttg cggaagcaga acgccgttat ggttctgata aaccggatct
     3121 gcgtaacccg atggaactga ccgacgttgc cgatctgctg aaatctgttg agtttgcagt
     3181 atttgcgggg ccggcgaacg atccgaaagg ccgtgttgcc gctctacgtg ttccgggtgg
     3241 cgcatcgctg acccgtaagc agatcgacga atacggtaac ttcgttaaaa tctacggcgc
     3301 gaaaggtctg gcttacatca aagttaacga acgcgcgaaa ggtctggaag gtatcaacag
     3361 cccggtagcg aagttcctta atgcagaaat catcgaagcc atcctggatc gtactgccgc
     3421 gcaagatggc gatatgattt tcttcggtgc cgacaacaag aaaattgttg ccgacgcgat
     3481 gggcgcactg cgcctgaaag tgggtaaaga ccttggtctg accgacgaaa gcaaatgggc
     3541 accgctgtgg gttatcgact tcccgatgtt tgaagacgac ggtgaaggcg gcctgacggc
     3601 aatgcaccat ccgttcacct caccgaaaga catgactgct gcagaactga aagctgcacc
     3661 ggaaaatgcg gtggcgaacg cttacgatat ggtcatcaat ggttacgaag tgggcggtgg
     3721 ttcagtacgt atccataatg gtgatatgca gcagacggtg tttggtattc tgggtatcaa
     3781 cgaagaggaa cagcgcgaga aattcggctt cctgctcgac gccctgaaat acggtactcc
     3841 gccgcacgca ggtctggcat tcggtcttga ccgtctgacc atgctgctga ctggcaccga
     3901 caatatccgt gacgttatcg ccttcccgaa aaccacggcg gcagcgtgtc tgatgactga
     3961 agcaccgagc tttgctaacc cgactgtact ggctgagctg agcattcagg ttgtgaagaa
     4021 ggctgagaat aactgatatg actcaaatac acgaaatcat tcgcgttgca tcaaggcggc
     4081 aactgagtga actcccatga gcatagataa ctatgtgaat gggatgagcg aaggcagtca
     4141 acgaagaggc agcgtgaagg ataaagtgta taagcgtccc gtttcgatct tagtggtcat
     4201 ctatgcacaa gatacgaaac gggtgctgat gttgcagcgg cgtgacgatc ccgatttctg
     4261 gcagtcggta accggcagcg tggaagaggg tgaaaccgcg ccgcaagctg ccatgcgcga
     4321 agtaaaggaa gaggtcacca ttgatgttgt cgctgaacaa ctgaccttaa ttgactgtca
     4381 gcgcacggta gagtttgaaa ttttttcaca tttacgtcat cgctatgcgc cgggcgtgac
     4441 gcgtaatacg gaatcatggt tctgtcttgc gctcccgcac gaacgccagg tagtcttcac
     4501 tgaacatctg gcttacaagt ggcttgatgc gcctgctgcc gcggcgctca ctaagtcctg
     4561 gagcaaccgg caggcgattg aacagtttgt aattaacgct gcctgaacag gcagggctat
     4621 ctggagatat ttttatggca ggtcatagta aatgggccaa caccagacat cgtaaagctg
     4681 cgcaggatgc taagcgcggt aaaatcttca ctaaaatcat tcgtgagctg gtaaccgcgg
     4741 ctaagctggg cggtggcgat ccggacgcta acccgcgtct gcgtgcggcg gtggataaag
     4801 cactgtctaa caacatgacc cgtgacacac tgaaccgcgc aattgcacgt ggtgtgggcg
     4861 gtgatgatga tgcaaacatg gaaaccatca tctacgaagg ttacggtcct ggcggcacgg
     4921 caatcatgat tgaatgtctg tctgacaacc gcaaccgtac cgttgctgaa gtgcgtcatg
     4981 catttagcaa atgtggcggt aacctcggta ctgatggttc cgttgcctat ctgttcagca
     5041 aaaaaggcgt gatctccttc gagaaaggcg atgaagacac cattatggaa gcagcactgg
     5101 aagcaggtgc tgaagacgtt gtgacctatg atgacggcgc gattgacgtc tacactgcat
     5161 gggaagaaat gggtaaagtg cgcgacgctc tggaagcggc aggtctgaaa gcagacagcg
     5221 cggaagtttc catgatcccg tccaccaaag ctgatatgga tgcagaaacc gcaccgaaac
     5281 tgatgcgtct gatcgatatg ctggaagatt gcgacgacgt gcaggaagtc taccataacg
     5341 gtgaaatctc tgatgaggtt gcagcaactc tctgatgggg cctgctaaac agcaaaacgg
     5401 agacgcgtga tggctattat tctcggcatt gacccgggtt cgcgcgtgat cggctatggt
     5461 gtcatccgcc aggtaggcag gcaactgtcc tacctgggta gcggatgcat acgcaccaaa
     5521 gtggatgatt taccgtctcg tctgaagctc atctatgcgg gcgtgacgga aatcatcacc
     5581 cagttccagc cagattattt cgccattgaa caagtcttta tggccaaaaa tgccgactcg
     5641 gctctgaaac tggggcaggc gcgcggcgtg gcgattgtgg cggcggtgaa tcaggaattg
     5701 ccggtatttg aatacgcggc acgtcaggta aagcaaacgg tggtaggtat tggtagtgcc
     5761 gaaaaaagcc aggtgcagca tatggtccgc accttgctga aactgcccgc taatccacag
     5821 gcggatgccg ccgatgcgtt agctatcgcg attacccatt gtcacgtcag ccagaatgca
     5881 atgcagatga gcgaatcgcg gctgaacctg gcgagagggc gactgcgtta agttataccg
     5941 cctcggtcag ttcaggctga ggcgtttcaa ctccctccgc gtgaatcaac accagacccg
     6001 gatgatgcca caaactggct ggcgtgacgg ttttacgctc ccacggaata gaacctaaga
     6061 accagaattt ccagaaagtg agttttgcat tcggattgct atttaacagt tctccaaacg
     6121 tttctatttc acccaccgga atgcatagcg cttctttata aatatcaaaa acaaattttg
     6181 aacagaactg gcgcgaagat tcgtatttaa aaccggtgtg gtaaagtttg cgtagccggg
     6241 agggaacctg ttcaacaatt cgttgttttt gttgttctgt cagcccagcg tctaatcgct
     6301 ttatagcata gcgttgatta gacgaacgtt taataaaacg agatagcgtg gtgatggttg
     6361 aaaggggaac ccggctttct gcaaccagga agtcttcacc gttatgaccg ataatgatcc
     6421 cgacgtgatt actccagcaa tttgatgcag ctgatatttg accaaataag gcagcaccta
     6481 tacatgtaaa gacgatatct ccaatttcat attc
//
LOCUS       DAZSBF010000224         6479 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZSBF010000224 DAZSBF010000000
VERSION     DAZSBF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.228"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHXWD_003593"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHXWD_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR7622400.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHXWD_003594"
     tRNA            1230..1305
                     /locus_tag="ACHXWD_003594"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHXWD_003595"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="ACHXWD_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHXWD_003596"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="ACHXWD_003596"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR7622401.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHXWD_003597"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="ACHXWD_003597"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR7622402.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHXWD_003598"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="ACHXWD_003598"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR7622403.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="ACHXWD_003599"
     CDS             complement(4869..5306)
                     /locus_tag="ACHXWD_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR7622404.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="ACHXWD_003600"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="ACHXWD_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
BASE COUNT         1731 a         1755 c         1420 g         1573 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccag
//
LOCUS       DAZSBF010000225         6459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZSBF010000225 DAZSBF010000000
VERSION     DAZSBF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.229"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            3..194
                     /locus_tag="ACHXWD_003601"
     CDS             3..194
                     /locus_tag="ACHXWD_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622405.1"
                     /translation="MNTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPEGNAPNYC
                     WWEVDPVDAANEAMSYHND"
     gene            227..445
                     /locus_tag="ACHXWD_003602"
     CDS             227..445
                     /locus_tag="ACHXWD_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR7622406.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            516..833
                     /locus_tag="ACHXWD_003603"
                     /pseudo
     CDS             516..833
                     /locus_tag="ACHXWD_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(866..1504)
                     /gene="ompW"
                     /locus_tag="ACHXWD_003604"
     CDS             complement(866..1504)
                     /gene="ompW"
                     /locus_tag="ACHXWD_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR7622407.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1744..1833
                     /locus_tag="ACHXWD_003605"
                     /pseudo
     CDS             1744..1833
                     /locus_tag="ACHXWD_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1861..2604
                     /locus_tag="ACHXWD_003606"
     CDS             1861..2604
                     /locus_tag="ACHXWD_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR7622408.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2634..3173
                     /locus_tag="ACHXWD_003607"
     CDS             2634..3173
                     /locus_tag="ACHXWD_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR7622409.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3278..3676
                     /gene="yciA"
                     /locus_tag="ACHXWD_003608"
     CDS             3278..3676
                     /gene="yciA"
                     /locus_tag="ACHXWD_003608"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR7622410.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3716..4435)
                     /gene="tonB"
                     /locus_tag="ACHXWD_003609"
     CDS             complement(3716..4435)
                     /gene="tonB"
                     /locus_tag="ACHXWD_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR7622411.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4526..6046
                     /locus_tag="ACHXWD_003610"
     CDS             4526..6046
                     /locus_tag="ACHXWD_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7622412.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6079..>6459)
                     /locus_tag="ACHXWD_003611"
     CDS             complement(6079..>6459)
                     /locus_tag="ACHXWD_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622413.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1689 a         1482 c         1543 g         1745 t
ORIGIN      
        1 ttattaacac tgaaaaatat aacgagtggg tgaaaaaatt ctctgagcag atttttaaaa
       61 ttactggcga cgagaatgtg gcaaaaaatg aattagaacc gtggacacct gaaggaaacg
      121 caccaaatta ttgctggtgg gaggttgatc cggttgatgc tgcaaatgaa gccatgagtt
      181 accacaacga ttaatgtcgg gaggccgccc gaaagggcgg taagaaatga ctacattatt
      241 cagaaaagaa tatccgcaaa aaagtagggc gacagaattt ttgtttctca ttctgtttat
      301 cgtattgatg ataccgatat cccctctaat ttttgtctgg gcaatcggga aaataattga
      361 gccagttact gaattgtata ccgacgttgt atgggcgtcg ttcaacacac tgcacaataa
      421 aattaatccg tataaggaaa actgatatgg caactttgac aaaaaaagaa cgggcatggt
      481 tgaacgaatt acaggaagtt cttgatcgct gtcctgtggt gtcatcctgc cgcgattgcg
      541 ttcttagtcc gtggctattg caccaccatc acgcgaaagg gacagctaag cgcggcggga
      601 tggttaagcc agcaacatta accgttgcat ttaaaaaagc ccgggattct gtggattaca
      661 actggcgtgc taatggcacc ccaccctctt tccatgagca gagatcttta tcagagcgat
      721 tgttcagaga gcagggggtt gataccaaaa ttttgctagg ccattcgaat caaaaaatga
      781 ccgatattta caacgatgta cgcggtaagg agtggaaaaa actggtcatt tgataaccag
      841 ttttgcagag gttttgcaga gaaaattaaa agcggtaccc ggcagagaac ataaacaccc
      901 acggatccag tcgtaccgag tcttttacgg tagtaacacc agatttgtac ttagccgtgg
      961 tatcgatatc catgtaccac actgacatgt taaccaacca gtcacggtta atcagataat
     1021 caacccccac ctgcccggca gctccccagt aatctttcag actgagatcg gaaagccccg
     1081 cctctttgcc atgatcgtta aatccattat caaagaaggt ggtgtagtta atacctgccc
     1141 caacataagg acggaatttg ctgccggcat caccaaaata ccactgcgcc atcagtgttg
     1201 gcggcagatg atgaacggtt gcaatatcgc cggtcgcccg ggtgccgatt ttatggcgga
     1261 acggcgtcgc tgccagtaat tccacaccaa tgttgtcggt cgccatataa gtaaacgtca
     1321 ggcccagttg cgtgttattg gtcacgctga atccacccag acttcctaac gtaccaccag
     1381 caccttctgt tggacgtacg gttgcagaac ctgcacgcat aaaaaattcg cctgcttcat
     1441 gcgcaaaggc actgccagag agaagagttg ttactgccaa agccgccact gttaactttt
     1501 tcatatccgc tccgtcgtta tggttataaa aacgtgacga atatacctac attggagtaa
     1561 caaatgatcc tacatagatc acattacata tgataattca ccctttattg atctggatta
     1621 atttagaagt tgcattgaga atcctggtta acgtgattta aatcaatttt tagatatttt
     1681 cgctacgcaa atattaactc gttcgccact tgccgcggtt tgcccacgcc tgaaggccag
     1741 caattacctc cgctttacaa agatatgctt ttccatacaa gacctgatgc tgccacccga
     1801 ggcttaacca gggtacaatt gcccgctaat taacacctgc ataaaactca aggagagtgc
     1861 atgtctatca cggcgcagtc cgtataccgt gacaccggaa atttcttccg taatcaattt
     1921 atgaccattc tgttggtatc gttgctatgt gcgtttatca cagtggtgtt agggcatgtt
     1981 ttctcaccca gtgatgcaca gcttgcgcag ctcaatgacg gcgtgcccgt tagcggcagt
     2041 agtgggttgt tcgacctggt tcagaatatg tcaccggaac aacaacaaat tttgctgcag
     2101 gcttcagcgg cgtccacttt ttcaggatta atcggtaacg ccattctcgc cggaggcgta
     2161 atattaatta tccagctggt gtctgcgggt cagagagtca gtgcgctgcg agctattggt
     2221 gccagtgcgc cgatattgcc caagttattt attctgattt ttctgactac ccttttagta
     2281 cagattggca tcatgctggt ggtcgttccg ggaattatca tggccatttt actggccctg
     2341 gcaccggtga tgctggtaca ggacaaaatg ggcatttttg cctcgatgcg tagcagtatg
     2401 cggctgactt gggcgaatat gcgtctggtg gcacccgcag tactgagctg gttgctggca
     2461 aaaacactgt tgctgctttt tgcctcttct tttgccgcat taaccccgga aattggtgcc
     2521 gtactggcga acaccttgag caacctgatt tcagccatat tgctcatcta tctgttccgc
     2581 ctgtatatgt tgattcgcca ataaccttaa gcatttgatt acggaatcgt aaaatgaagc
     2641 agtttcttga ttttttaccg ctggttgtct ttttcgcgtt ttacaagatt tatgacatct
     2701 atgcggctac tgcggcgctg atcgtcgcca cggcgattgt gcttatctat agctgggttc
     2761 gctttcgtaa ggttgagaag atggccctga tcacttttgt tctggtggtc gtcttcggtg
     2821 gcttgacgct gttcttccac aatgatgagt ttattaaatg gaaggttaca gtcatttatg
     2881 ctctgtttgc gggagccctg ttagtcagcc aatgggtgat gaaaaagccg ctaattcagc
     2941 ggatgctggg taaagaactc acgctgccgc aaccggtatg gtcgaagcta aatctggcct
     3001 gggctgtttt ctttatcctt tgcggtctgg caaacatcta catcgcattc tggctgccgc
     3061 aaaatatttg ggtcaacttt aaagtctttg gcctgaccgc ccttacctta atcttcacat
     3121 tgttaagcgg tatctatatc taccgccaca tgccgcagga agataaatcc taaccagact
     3181 gccagtcctt cggggctggc gctgtatctc ctctgttctc ctcaggaaaa tcatagtagc
     3241 atcgcgcctg tgattttcct tttaagtcgg ttttaccatg tctacaacac ataacgtccc
     3301 tcagggcgat cttgttttac gtactttagc catgcccgcc gataccaatg ccaatggtga
     3361 catctttggt ggttggttaa tgtcacaaat ggatattggc ggcgctattc tggcaaaaga
     3421 aattgcccac ggtcgcgtag tgactgtgcg ggttgaagga atgactttct tacggccggt
     3481 tgcggtcggc gatgtggtgt gctgctatgc acgctgtgtc cagaaaggga cgacatcggt
     3541 cagcattaat attgaagtgt gggtgaaaaa agtagcgtct gaaccaatcg ggcaacgcta
     3601 taaagcgaca gaagcattat ttaagtatgt cgcggttgat cctgaaggaa aacctcgcgc
     3661 cttacctgtt gagtaatagt caaaagcctc cggtcggagg cttttgactt tctacttact
     3721 gaatttcggt ggtgccgtta attttaaaca ggatattcac cacaatccca ctgcctggct
     3781 tacccggctc ataacgccat ctgcgcatcg catttttcac ctcacgctca aacatgttcg
     3841 caggcttggc tgagaggatt tgtacgttat ccacgcgacc atccggcgtg acgtcaaatt
     3901 taactttaac ctgcccttca atgcgcaatg cctgtgctcg tgccggatac tgcggctgat
     3961 tacggcttaa tgcgcgtggt cctgaagcca cactggtaac cggcttgctg gttgcagccg
     4021 ttgccgtact tgatgtgggg cgcgcgggtg ccgtattttc aaacggtgat gccggacgcg
     4081 actctacggg tttgacatcg cgttttggct gctcctgtac ctttttcacc ggcttcggtt
     4141 ttggcttagg tttcggcttc ggcttttcaa tgaccaccgg tgcttctttt ggcggttcgg
     4201 ggatcggctc aggttccggt tctggctcta ccaccggctc cggtggcggc tgaacggctt
     4261 gtggcggttc gagatcagca ggcgcaacca tcgtgacaga aattggctgc gcaggcgcag
     4321 gtagttcaat aacctgatgt accgaggtat agagcagacc cgccacaaca gcaccatgaa
     4381 tgcagaccga aagtaacgtc ggccagggga agcggcgagg taaatcaagg gtcattgtat
     4441 tgtagctatt cggcattgta gcattagcca taacgtttat aagttcatct ttaaatgata
     4501 atcaattgca tgggagttta tcaccgtgag aaccgtaata acatacttga gattctcatc
     4561 agccatacaa ggcgctgaag gtgcagattc aaccagacga caaaacgacc tgttcaagca
     4621 gtggctgaag aagaatggcg atgctcaaat agtagcgtct ttcagtgatg aagggttgag
     4681 tggttacaaa ggtaaacacc ttactggtca gtttggtgac atgttagccc gtattgaggc
     4741 tggggagttt ccagaaggta caattctgct agttgagagt atcgaccgta taggccgatt
     4801 agagcatctt gaaacagaag ccttgatgaa ccgcatctta ggcaacggga tagagattca
     4861 cacattgcaa gatggcctta tctacacaaa ggatgcttta gccgatgacc ttggcataag
     4921 catcatccag cgtgtcaaag cctacatagc gcatcagaaa tctaagcaga agtctttccg
     4981 tgtttctcag aaatgggggc aacgtgcaaa acttgccctt gctggtgaac aacgtcttac
     5041 aaagatggtt cctggatgga ttgaccctga gacttttaaa ctcaatgaac acgctgagac
     5101 tgtgagattg atattcaaac tgctgctaga tggtgaaagt ctgcataaca ttgcacgtca
     5161 ccttcaaagc aacggtataa agtcgtttag tcgccgtaaa gatgctaatg ggttctctgt
     5221 tcactctgta cgcaccattc taaggtcaga gacaacaata ggcacgttac cagcatcaca
     5281 acgtaatgac cgccccgcta taccgaacta ctacgaaggt gttgtagata taccaacgtt
     5341 caataaagct caagagattc tcgacaagaa tcgtaaaggc cgtacacctg caagtgacaa
     5401 cccactaacg attaacatct tcaaaggtct gtttaggtgt cagtgtgggg ctagtgtcca
     5461 tcctaccgga acaaagaata agtatgctgg ggtctacagg tgcaataacc acttagacgg
     5521 tcgctgtgat gttccaccgt tgaagcgtaa accgtttgac cgatggatga ttgataattt
     5581 tctggggatg attgacgtgg ggaatgatgg agaatcagag agaaagattg cagcgttaca
     5641 gcatgaggtt gaaattgtca cagccagaat caagaaagct accgccctac ttcttgagat
     5701 ggatgatatt gatgaactaa aaattcagct taaggaactg aaccagaagc gcacagaact
     5761 acagaccacg attgataaca tgaggcgtaa ggcttcactc actgataagg aactacccca
     5821 gcttaaagat attgacctta tgactaaagc gggtcgtgtc gagtgtcagt tgattttgtc
     5881 caagcatcta aaagggctta cattgggtaa ggattcggta actgtaacgc tacagaacga
     5941 cactgaaatc accataccta caaacccgtt accgctaaat gatgggtcac caatctttga
     6001 aattgctgat aaagagctac tggatattga cgcttatcaa ctgtaatttt caggggctat
     6061 gaagcccctt tttatgtctc aataagtgcc cttcttgtta gggttcacag agtactttcg
     6121 cagagcatta cccggcttaa gcacctttga agcttgctca ttagcggcat ctgagaaggt
     6181 gctctcacca tttttaacgc taacgctctt agagcacgtt acacgggctt ttagcgcttc
     6241 atctgataac tcacttgccg aatagccaaa aagctcttta caggtcagca tagggcttcc
     6301 aaggctcgtt gtagtgtttc cggtgttctt tttactggta gcaccgccat cattcgtggt
     6361 agcggtcttt ccagtagcag ccgaaacatc agtctgacct acgattgaac gaaactctac
     6421 accttggaag gtcatctgaa tacgaagacc gttagcaat
//
LOCUS       DAZSBF010000226         6410 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZSBF010000226 DAZSBF010000000
VERSION     DAZSBF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.231"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            67..1122
                     /gene="phoE"
                     /locus_tag="ACHXWD_003612"
     CDS             67..1122
                     /gene="phoE"
                     /locus_tag="ACHXWD_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR7622414.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1161..1562)
                     /gene="crl"
                     /locus_tag="ACHXWD_003613"
     CDS             complement(1161..1562)
                     /gene="crl"
                     /locus_tag="ACHXWD_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR7622415.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="ACHXWD_003614"
     CDS             complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="ACHXWD_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR7622416.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="ACHXWD_003615"
     CDS             complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="ACHXWD_003615"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR7622417.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3675..5132
                     /gene="pepD"
                     /locus_tag="ACHXWD_003616"
     CDS             3675..5132
                     /gene="pepD"
                     /locus_tag="ACHXWD_003616"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR7622418.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5189..5803)
                     /gene="prfH"
                     /locus_tag="ACHXWD_003617"
     CDS             complement(5189..5803)
                     /gene="prfH"
                     /locus_tag="ACHXWD_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR7622419.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5800..6410)
                     /locus_tag="ACHXWD_003618"
                     /pseudo
     CDS             complement(5800..6410)
                     /locus_tag="ACHXWD_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1630 a         1643 c         1578 g         1559 t
ORIGIN      
        1 actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt aaaatcagga
       61 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgcatct
      121 gtacaggctg cagaaatata taataaagac ggtaataaac tggatgtcta tggcaaagtt
      181 aaagctatgc attatatgag tgataacgcc agtaaagatg gcgaccagag ttatatccgt
      241 tttggtttta aaggtgaaac acaaattaac gatcaactga ctggttatgg tcggtgggaa
      301 gcggagtttg ccggaaataa agcggagagt gatactgcac agcagaaaac gcgtctcgct
      361 tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
      421 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ttcctcggcg
      481 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
      541 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
      601 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggc
      661 ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa cgagcagaac
      721 ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct gaaatacgat
      781 gccaataata tttatctggc aactttctat tctgaaacac gcaaaatgac gccaataact
      841 ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca gtttgacttt
      901 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga aggtatcggt
      961 gatgaagatc tggtcaatta tatcgacgtc ggtgctacgt attatttcaa caaaaatatg
     1021 tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt gaatattaat
     1081 aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatggatatt gccggatgtg
     1141 atacatccgg cacatttcac tcacgccgtt aacttcaccg gttcatcacg aaaatcatcc
     1201 gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc gtgaaactca
     1261 cgtagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac actcttccag
     1321 tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct
     1381 tcaagctcca tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg
     1441 catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac
     1501 gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact cggtaacgtc
     1561 attgcaatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaatttt agcaaatttt
     1621 taacacaagc gtttttcgat ccagccggtg atttcctgaa gacctttgtc aaaattccga
     1681 tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga ggtgattaag
     1741 cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa cattggcgtt
     1801 gggcagcgac gccccagcaa tccttgcact tttaatgaat agcgattcag ctccacgcgt
     1861 aacgcttcgt cggaagcatc atgcatcccc aaacgactcg ccagaacgtc aagatacatt
     1921 tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga
     1981 caggcaaccg ctttcagacg cggcgattca agatatgcca gacgcacggc aacgttagcg
     2041 ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcaga
     2101 gcctttaaaa cgtgctgatg caacaggctg gagtcctggg tgagcttcca ttttgatgaa
     2161 aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc aaaataacgt
     2221 tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat taatactgtc
     2281 gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt
     2341 acggtaaact ccatctgccg catcgtgccc ggcagacgct gggcggcttc ttcataggca
     2401 cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggca
     2461 gcaatgttgt acaacgtagc cgcatgcagc cagtgacgac cactgagttg cggatcttct
     2521 tccgcacagg ctttttgttg ccacaccatc gcctggatgg cccactcata aatccagttg
     2581 ccaccacggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc
     2641 atcacaatac gcgcctggac gtcgaggatt tcacgtgggt caatgccgcg ccagatccac
     2701 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc
     2761 acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta
     2821 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga
     2881 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc
     2941 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca
     3001 cggctgttca atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg
     3061 accagccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc
     3121 aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc
     3181 ttctgcgcgt tttagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca
     3241 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc
     3301 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     3361 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     3421 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     3481 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     3541 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     3601 tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga
     3661 caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt
     3721 ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca
     3781 ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc
     3841 tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa atcggtcgtc ttacaggccc
     3901 acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg aaagatccta
     3961 tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcagata
     4021 acggcattgg tatggcgtct gcgctggcgg ttctggctga cgaaaacgtg gttcacggcc
     4081 cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg ttcggcttac
     4141 agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa
     4201 tctacatggg ttgtgcgggg ggtatcgact tcacctccaa cctgcattta gatcgtgaag
     4261 cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa gggggtcact
     4321 ccggcgggga aatccacgtt ggcctgggta atgccaacaa actgctggtg cgcttcctag
     4381 cgggtcatgc agaagaactg gatctgcgcc ttatcgattt caacggcggc acactgcgta
     4441 acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgtcc
     4501 tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca gagaaagaga
     4561 aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg attgcgaaat
     4621 ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc cgcaactcag
     4681 acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc atgactgata
     4741 ataacgtaga aattcactgt ctgatccgtt ctctgatcga cagcggtaaa gactacgtgg
     4801 tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg aaaggcgcat
     4861 atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt gaaacctatc
     4921 agcgtctgtt caacaaaacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc
     4981 tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact atcaccggtc
     5041 cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg acactgctga
     5101 ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt
     5161 aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc cctgtaaatg
     5221 tccgtctcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc gatttcagca
     5281 ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat cgtgccagcc
     5341 gcttgttagc atgctgacta cgctctgact gaaccttcac gctaataccg gatgccaaat
     5401 gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc cccgacgaac
     5461 gcaacgtctc ataacggatt gcatccgatt gttcctgctc atcagcggta aaacgcccaa
     5521 tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga caaatccact
     5581 gaatagtgcc gcaccagctt tcgcttaatg cccatgcgtt atcgccatcc agagaaacca
     5641 gagctgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccaacacc gttaccgcga
     5701 cgtcttgtcg ggcagcttct ttaattaatc gctccagcgc ctttttcact gcgagacaac
     5761 attcttccgg cccctgcgct gaggagagtt gtagcaagat catccttttt tccctccact
     5821 gtttttgagc gttagcaccg gacgcagccg cgcgacagga ataattaaac cagcctgcac
     5881 cagacattgc accacgcttt cagccgattt ataggcttgt ggcgcttctt caaagatgag
     5941 ttgtttatcg cgacaaatta cccgactgcc aagttcagta cgtgagagct gcgtcgctgt
     6001 gtatttcgct gccagacgcc ctttacactc ggtgcgcccc catttacgcc cagccccatg
     6061 cgccagcgag tggagcgttt tttcgttcgc aacaggctgt acaagctagg agtaatcacc
     6121 gagtgaaccg ggaataatca ccagaccgtt gtcatccggc gtggcacctt tacgatgcaa
     6181 ccagccctgt tgatcgccga tttgacacgc gctaacaaag ttatgcgcca catccagaac
     6241 cggacttccg gtggccttaa cctgttgcat tatgcgtaaa gagatcagct ggcgattaat
     6301 acgcgcaaac gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt cgtcactgcc
     6361 ttcaggcaaa ccatgatgcg aaaacgaggc aatatgccgc tgtaaaatag
//
LOCUS       DAZSBF010000227         6376 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZSBF010000227 DAZSBF010000000
VERSION     DAZSBF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.232"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..357
                     /gene="ydjO"
                     /locus_tag="ACHXWD_003619"
                     /pseudo
     CDS             1..357
                     /gene="ydjO"
                     /locus_tag="ACHXWD_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347595.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(361..1752)
                     /gene="tcyP"
                     /locus_tag="ACHXWD_003620"
     CDS             complement(361..1752)
                     /gene="tcyP"
                     /locus_tag="ACHXWD_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR7622420.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1885..2475)
                     /locus_tag="ACHXWD_003621"
     CDS             complement(1885..2475)
                     /locus_tag="ACHXWD_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR7622421.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2638..3306)
                     /gene="hxpB"
                     /locus_tag="ACHXWD_003622"
     CDS             complement(2638..3306)
                     /gene="hxpB"
                     /locus_tag="ACHXWD_003622"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR7622422.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3453..3989
                     /locus_tag="ACHXWD_003623"
     CDS             3453..3989
                     /locus_tag="ACHXWD_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR7622423.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4030..4890)
                     /locus_tag="ACHXWD_003624"
     CDS             complement(4030..4890)
                     /locus_tag="ACHXWD_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR7622424.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4996..5286)
                     /gene="ghoS"
                     /locus_tag="ACHXWD_003625"
     CDS             complement(4996..5286)
                     /gene="ghoS"
                     /locus_tag="ACHXWD_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR7622425.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5387..6316)
                     /gene="pfkB"
                     /locus_tag="ACHXWD_003626"
     CDS             complement(5387..6316)
                     /gene="pfkB"
                     /locus_tag="ACHXWD_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR7622426.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1651 a         1596 c         1562 g         1567 t
ORIGIN      
        1 ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa gtttccttcg
       61 ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga
      121 agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg
      181 tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg tattgataag
      241 ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat cgccgcgatg
      301 gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc agcataagaa
      361 ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt
      421 cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta gcgccgtacg
      481 gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc
      541 catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc
      601 tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt
      661 aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc
      721 gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc
      781 cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt
      841 aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt
      901 aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac
      961 aacgaaactt cccagtttga tgatgtcttg caagttagaa cctgcaacca ctttggtcat
     1021 tagagccaga acgccgtaag gggtcaactg catgacaagg cgaaccagtt tcatcaccca
     1081 gctttgtagg gtatcgatag cggctaagac gcgttcacct ttcggcgcat catccttcag
     1141 cagtttcaat gcagctacgc cgaggaatgc ggcaaaaatt accacgctga taattgacgt
     1201 cggattggct ccggtaagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg
     1261 cggaacgctc agatcagaga ctttaccaac atagttactt tcaatggcgt tcagacgtgc
     1321 agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag
     1381 cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg tcagaaaact
     1441 gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa
     1501 gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa
     1561 ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag
     1621 gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt
     1681 atggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa
     1741 tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag
     1801 tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat
     1861 tccagaaagg tgatttttgc ctttttagta ttcaacctga tgcttaataa gccgatgaaa
     1921 ctggatctgc aaggtattga tcatttgcga tgaccaacga acagcgctgt tctcgggtaa
     1981 tgaatggggc atccataccg accagacaaa taatgccatg cagataaaac gttccagttg
     2041 gttgcccttt tggggaacca ggataggcaa gcggaaacgc cagcggcatg gccagagcag
     2101 gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac ccagcaccat
     2161 tccttgtagc gcatcagccg gaatgaacca gccttccgga accttaaggt aaaaggttgc
     2221 cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc
     2281 gatcggtttt gatatccatt ttaatcgctg cccaagaaac gactttgggt gatcgatgtc
     2341 cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag
     2401 cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg
     2461 accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta
     2521 tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc
     2581 agttgaatgc aaattcagat aaagaaccag gggcagcgtg atcactgccc ctggtcatta
     2641 accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac
     2701 aaaacgtgga tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc
     2761 tttagaagcg atcatgccat ttaccgagtc ttccagcgcc acgcaggtca gtgggtcaac
     2821 gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt
     2881 ttcagccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc
     2941 cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa
     3001 cgcaacagct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc
     3061 acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc
     3121 gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg
     3181 acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat cccgtaaagg
     3241 ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt
     3301 tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt
     3361 aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa
     3421 gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg
     3481 ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc tccacattga
     3541 tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca
     3601 tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc
     3661 tgttctggta taactccccg acccctaatt ttaatggtgg cgtgaacgtt atgttctggg
     3721 tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc
     3781 gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag
     3841 gggaagaggg gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta
     3901 ttttcctgca gtttttctca ctatatatcc tgccggttat ttgtattgct gcaggttatg
     3961 tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg ccattatcct
     4021 caacccatat catgctgcta ataatctatc caatgactgc tgagcaataa ccaaatgctg
     4081 accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc
     4141 aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg
     4201 ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg
     4261 gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg
     4321 caacagagaa ggctgcagtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc
     4381 gtcgatattg ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg
     4441 ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga
     4501 gagcgagtta tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc
     4561 aatttgctga ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc
     4621 catcaccaga aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac
     4681 ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag
     4741 ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc
     4801 atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc
     4861 gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca
     4921 taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta
     4981 ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaagtcg tgatggtgat
     5041 ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc
     5101 ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc
     5161 atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat
     5221 ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact
     5281 ggccatagtt cacccctttg ttatcacatg acttttgagc aaagtcggag aaacgcgttg
     5341 ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt
     5401 aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg
     5461 ctgcactccc cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca
     5521 gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca
     5581 ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac
     5641 caagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc
     5701 ggacatcgtc cggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag
     5761 gcttaaccaa ctcgatgtta ccaattgcca gtgctgcact taacgcttcg ccagaactgt
     5821 cgacgatgca gcggatccct tgtttttgcg aagcggaaat cagttgggtt aatttttcca
     5881 gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca
     5941 gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa
     6001 aacgatactg ctcaccgctt gcttccacat gtacatgtaa attctgccgg gtccagtctt
     6061 tggcttctac agtagcgacg gggacatttt catccgccaa cagtgaaacc aggtgttcgc
     6121 cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc
     6181 gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc
     6241 cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg
     6301 tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcg
     6361 gaaagtgaag aaatta
//
LOCUS       DAZSBF010000228         6316 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZSBF010000228 DAZSBF010000000
VERSION     DAZSBF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.233"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="ACHXWD_003627"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="ACHXWD_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR7622427.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHXWD_003628"
     CDS             complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHXWD_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR7622428.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="ACHXWD_003629"
     CDS             2736..3797
                     /locus_tag="ACHXWD_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR7622429.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHXWD_003630"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHXWD_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR7622430.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..6295
                     /locus_tag="ACHXWD_003631"
     CDS             5678..6295
                     /locus_tag="ACHXWD_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR7622431.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1701 a         1594 c         1513 g         1508 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctggggtat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgctag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 ggggtctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta tttgagtggg
     6301 ataccagagg attatg
//
LOCUS       DAZSBF010000229         6256 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZSBF010000229 DAZSBF010000000
VERSION     DAZSBF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.234"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHXWD_003632"
     CDS             122..952
                     /locus_tag="ACHXWD_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR7622432.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHXWD_003633"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHXWD_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR7622433.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHXWD_003634"
     CDS             complement(2081..3541)
                     /locus_tag="ACHXWD_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR7622434.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHXWD_003635"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHXWD_003635"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR7622435.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHXWD_003636"
     CDS             5146..>6256
                     /gene="gntP"
                     /locus_tag="ACHXWD_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR7622436.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISA"
BASE COUNT         1480 a         1619 c         1588 g         1569 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcac caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttaat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctg
//
LOCUS       DAZSBF010000230         6228 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZSBF010000230 DAZSBF010000000
VERSION     DAZSBF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.235"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(112..642)
                     /locus_tag="ACHXWD_003637"
                     /pseudo
     CDS             complement(112..642)
                     /locus_tag="ACHXWD_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(985..1635)
                     /gene="zinT"
                     /locus_tag="ACHXWD_003638"
     CDS             complement(985..1635)
                     /gene="zinT"
                     /locus_tag="ACHXWD_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR7622437.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1892..2527)
                     /gene="msrQ"
                     /locus_tag="ACHXWD_003639"
     CDS             complement(1892..2527)
                     /gene="msrQ"
                     /locus_tag="ACHXWD_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR7622438.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            complement(2528..3532)
                     /gene="msrP"
                     /locus_tag="ACHXWD_003640"
     CDS             complement(2528..3532)
                     /gene="msrP"
                     /locus_tag="ACHXWD_003640"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR7622439.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(3641..4054)
                     /gene="hiuH"
                     /locus_tag="ACHXWD_003641"
     CDS             complement(3641..4054)
                     /gene="hiuH"
                     /locus_tag="ACHXWD_003641"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR7622440.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4187..4858
                     /gene="hprR"
                     /locus_tag="ACHXWD_003642"
     CDS             4187..4858
                     /gene="hprR"
                     /locus_tag="ACHXWD_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR7622441.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4858..6216
                     /gene="hprS"
                     /locus_tag="ACHXWD_003643"
     CDS             4858..6216
                     /gene="hprS"
                     /locus_tag="ACHXWD_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR7622442.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
BASE COUNT         1755 a         1270 c         1424 g         1779 t
ORIGIN      
        1 cacagtttct gctcctcctt gttgatagta gaaaggatgc attgtgcact tgcgtaaaca
       61 tttatgacgc gttgatgtac atggttcaca tttccttgtc aggggatcga cttatttgcg
      121 gcgatgcatc attcgtaaca gagtattatc tttctgaatg tagtgatgaa agagtgctgc
      181 gccagcgtga gctgcgatta aaaagtaacc tatatttgcc taggtttcgt gaatattttt
      241 tatcagtgct ttaatttcgc tgtttggggt aacaaaggga gacacattga aaccaaggaa
      301 actccacgat tttccactgt aagccatcaa agcaatcccc agcagaggaa gtgcaaggaa
      361 ggttatataa agcatgatgt gcattagcga agccgctttc atttgccagg caggtggcgg
      421 tggtacaata gaaggatcac tataacggtg ttttataatc aggcgtgaaa acattaacac
      481 ccaaacgaat atacccgcat tgtaatgtgt ttctcgcatc agcagataag tactactacc
      541 tttaggaaac cagccacgga gttccatcgc ggcataggtt attgcaacga aaagcagcgt
      601 tatccaatgt aaataaattt gagtttttga gaatcggttc atttttgtag ctcctgtaat
      661 ttttacagtc aagctatccc acgaacctta aaaattcctt atacagatgt ggttttaaga
      721 atttgttatt taaaaaattg atagtgcaca ttgtaaccag ccaatataag caatggcgtg
      781 agcacaggta tctttctctg ttggccgtat tgtgtatgga atccgttatt ggttaactca
      841 atgcgggtta gcttattaat atacagacac gtcatagtga tgagcgtgtc tgtagctagc
      901 ttgcgttcag tggcgtttgt ggatggacca gtggcagtta tgatgactta ctaaagcggc
      961 atcgaggcgt tatcatgaga atactcaatg agacatcatt tcctcgacca cttcttcgct
     1021 actcaactga tatggataat acgttggcca gttttccatt tcatttaata aaaattgctg
     1081 tgaatcatta cccataaaaa tgtgaaaatg agatgatttt cgtggtgcaa ttatatgatc
     1141 gctaaattgt atatatttag gggctttgct ttcaggatct ttacattcga ataagtaacg
     1201 aacgcctttc ttgcctgatt tataggtgag tattttgtat ccatcgtaat catatttaca
     1261 ggatgttgtt tcattatttc tatggaattc aacaatgccg tcctcaatgc caatcatctc
     1321 gatatctgtt gcataacctt tgtgataata atctttaatt tcagcaaatg ttttaatttt
     1381 atctgcatcc gctttcttct gaaagacggg gtcaagtttg ccactttgca gtaaaggata
     1441 aacggattgc cagactccat cccagtcact gagcgttcgg ttttgtacat tggcatcatc
     1501 aaaaacacca ttagcagctt tttgttcgac ctctgttaag ggtttgccgt gtgagtgatg
     1561 accatgcgaa aaggcaggag cgctaacaat aaagacacct aaagcaacag ccagtttgtg
     1621 aagacgaatt gccaaaacag tttcctccag agtcattgta tatgatgtgt aatgttatat
     1681 tgtaacatat ggcaaaatgc aacaactctt aggagatgag agcatagttt tctcaggtgt
     1741 acttatctat cttattgaaa taaaatgaat tatgttgata atttgaggtt gatgacaaac
     1801 gaaacataac ctgatgtgat ctgcgcaggc cggacagggc gctcgcgcca catccggcag
     1861 ggataacgcg tactttgtcg gcaatatgaa gttatacaga taatttgttg tgaacctgct
     1921 tgcgtaaccg cttaaaaagg gaacgcaact tcttataccg taaggctaaa agcagtacag
     1981 ccagcccagc gtagatgagc ggctgcggtg aaataatctt cactgaccac agataatgta
     2041 ttggggccag gatcgcgaca agatagacga aattatgcaa ctgttgccag tgcttgccga
     2101 gttttcgctg catcgcctgg gttgaagtaa acgctaaagc aagcaaaata acccagctga
     2161 taattcctaa cgttaaataa ggccgggtaa ttaactcctt gcctaataac gccagattat
     2221 tcacgcccag ctccagcaat gcgtaactgg ttaaatgcag tgtcgcccag gcaaagcacc
     2281 ataagcctaa caggcggcga gtgcgtatca ataacggctg ttttgcgtag cgtgccagag
     2341 gtgtgattaa caaggtcgcc agcaaaaatt tcagtgcagt gcgaccggta aaatgctgaa
     2401 tatctttcac cggatcggca cccagtccac cgtgattgat cgcccagacc agccagagaa
     2461 acggcaacaa tccggcaaga tgcaggctaa ctttcagcca tgtcacctgt tttgctgtca
     2521 gacgcattca gaaattctcc cgcaaatcca ggccacgata cagcgatgcc acctggtcgg
     2581 cgtaaccatt aaacagtagc gttggctggc gctgaacatc gaggatgccg cctgaaccaa
     2641 taaatcgttc ggtagcctgt gaccagcgcg ggtgatcaac atgcggatta acgttggcgt
     2701 aaaaaccgta ttcgtcaggc gctgccagat tccaggtggt tggcggacgc tcgcgggtca
     2761 gcttaatact gactatcgat ttaatccctt taaagccata tttccacggc acaatcagtc
     2821 gtaccggcgc gccattttgt ggcggtaacg ccttgccata aacacccacg gtcatcagtg
     2881 tgagcggatg cattgcttcg tcgagacgca atccttcgac ataaggatat ttcagcccgc
     2941 cgccgataaa gcggtcctgc tggcctggca tttgttccgg tgcataaatt gtttcgaaag
     3001 cgacatactt cgcattgctg gtgggttcgg caagcgccag caatttgtgc agcggaaaac
     3061 caatccacgg caccaccatc gaccatgcct ccacgcagcg catacgataa atacgctctt
     3121 ccagcgggaa gcgacgggtt aaatcatcgt gatcgagggt caatggtttt gccacttcgc
     3181 cgctgatttt cagtgtccat ggatcggttt tcaggctacc agcattagcg gcgggatcgg
     3241 ctttatccag cccgaattca tagaagttgt tataaccgga gactttatct actggcgtca
     3301 gtggcagatt attttgccag gcggtaggct tgctgaactc cagcggtttt ccggcggggg
     3361 cgggcgggcg atcgttccct ttaaaccagc taagcagatc ggcatgcgca gcgtgaggca
     3421 aagaaagtgc agctgcgctg atgcccagtg cttttaacac ctgtcgacgc ttcataaaga
     3481 ataccgactc ggccgtaaca tctgattctt ttaaaaattg attctttttc ataagccatc
     3541 acactttttt gtcgttagcc agcaagagcc gtttactgtt aatctgtcag ggagtgtcct
     3601 ccctaacagt agcagagatc tgcttttaca tatttatgca ttaactgcca cgataggttg
     3661 aatacccata ttggctaagt aataaaggca catgataatg ctcgttcact ttattaatat
     3721 gaaattcaac ggggatctca gggaagaaac tttcaagatt ttgtttcttg aaatagtccc
     3781 cggttttaaa tacgacacgg taatcgcccg tagttgcagt ttgctcgggc cacagtgcct
     3841 taattcgtcc atccttatct gttttggcgg tattaagttg taaccagccg ttgtccgctt
     3901 tcttttcaag agtgactgtc acgtcggcag cgggttttcc ggtttgctgg ttcaaaatgt
     3961 gcacgctaag aatgttttgt tgtgcggcat aaaccaaaga aggtaatgaa aatgctgccg
     4021 ttactacgga gagtactaaa taacgcttta acatgtttat atccttgtca tgtgaatgag
     4081 ttttgaggat agcgaaaaag cggccagggt aaccttacag cagcattaca attttgtaat
     4141 gaactaggct gtttctataa catatgattt atggcatatt attttcatga agattttact
     4201 tattgaagat aatcaaagga cccaggagtg ggtaacgcag gggctttccg aagcgggtta
     4261 tgtcattgat gccgtttctg atggcagaga tgggctttat cttgcgctga aggatgatta
     4321 tgcattgatc attctggata ttatgcttcc gggtatggat ggctggcaga tcttacaaac
     4381 gttaagaaca gcaaagcaaa cccctgttat ttgccttact gcaagggatt ctgtcgatga
     4441 cagagtcaga gggctggaca gtggagcaaa tgattatctg gtaaaacctt tttcattttc
     4501 tgagttgctg gcaagggttc gggcacaatt aaggcaacat cacgctttga attcaacatt
     4561 agaaatcagt ggcttaagaa tggactctgt aagtcaaagt gtgagcagga acaatatcag
     4621 tattacactg acgcgcaagg agtttcagtt actttggcta ctggcctcca gagctggcga
     4681 aattataccc agaacggtta ttgcgagtga aatttgggga atcaactttg atagtgatac
     4741 caatacggtg gatgtcgcca ttcgcaggct ccgcgcaaaa gttgatgatc cttttcctga
     4801 aaagctaatc gccacaatcc gggggatggg ctattcattc gtagcggtaa aaaaataatg
     4861 aaaagattat ctataaccgt ccgtttaacc ttgcttttta tattgctgct gtctgttgct
     4921 ggcgctggaa ttgtctggac tctctataat ggcctggcaa gtgagttgaa atggcgcgat
     4981 gatacaacac tcattaaccg gacagcacag atcaagcagt tgttaattga tggggtaaat
     5041 ccagatacgt tacctgtata ctttaaccgg atgatggatg ttagtcagga tatcttgatc
     5101 attcatggtg atggcatcaa taaaattgtt aaccggacaa atgtcagtga tgacatgtta
     5161 aataacatac ctgctagtga gacaatcagc gcagctggca tttacagaag cattattaat
     5221 gatactgaga ttgatgcttt acgaattaat attgatgaag tttcgccatc attaacggtt
     5281 actgtggcta aattggcttc agccagacat aacatgcttg aacagtataa aattaatagc
     5341 attataattt gcattgtcgc cattgtactt tgctcagtat taagtccgct gttaatcaga
     5401 acgggattac gagagatcaa aaagttgagt ggtgtaacgg aagcgctgaa ttataacgat
     5461 agccgggagc ctgttgaggt tagcgcatta ccgagagaac taaaacctct tgggcaggcg
     5521 ttgaataaaa tgcatcatgc tttagtcaaa gattttgagc gtctaagtca atttgctgac
     5581 gatctcgctc atgaacttag aacgcccatt aatgcattac tgggtcagaa tcaggttacg
     5641 ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa ttgccggtaa cattgaagag
     5701 ctggaaaata tttcgcggtt aacagagaac atactgtttc ttgcccgggc agataaaaac
     5761 aatgttttgg tgaaactgga ctcgctttct ctcaataagg aagtcgaaaa tttgttggat
     5821 tatcttgaat acctttcaga cgagaaagag atttgcttta aggtcgagtg caatcagcaa
     5881 atctttgcgg ataaaatttt actgcaacga atgttatcga atcttattgt taatgccatt
     5941 agatattcac cagaaaaatc gcgtattcat ataaccagtt ttcttgatac caacggctat
     6001 cttaatattg atgtcgccag tcctggaacg aaaattcatg agcctgaaaa actcttccgt
     6061 agattttggc ggggagataa ttcgcgtcat tccgtaggtc agggacttgg tctttcttta
     6121 gtcaaagcga ttgccgaatt acatggggga agtgctacgt atcactatct caataagcat
     6181 aatgtgttcc ggattatgtt accgcaaaga aattaacata gggcttca
//
LOCUS       DAZSBF010000231         6184 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZSBF010000231 DAZSBF010000000
VERSION     DAZSBF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.236"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1447
                     /locus_tag="ACHXWD_003644"
     CDS             <1..1447
                     /locus_tag="ACHXWD_003644"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR7622443.1"
                     /translation="LSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADILATEHF
                     SDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLET
                     VLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEA
                     LARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVD
                     RIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDV
                     LPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQ
                     GVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLAL
                     GVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLP
                     DNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1483..1686)
                     /gene="ydfZ"
                     /locus_tag="ACHXWD_003645"
     CDS             complement(1483..1686)
                     /gene="ydfZ"
                     /locus_tag="ACHXWD_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR7622444.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1863..2549)
                     /gene="rspR"
                     /locus_tag="ACHXWD_003646"
                     /gene_synonym="ydfH"
     CDS             complement(1863..2549)
                     /gene="rspR"
                     /locus_tag="ACHXWD_003646"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR7622445.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2638..3384)
                     /gene="ydfG"
                     /locus_tag="ACHXWD_003647"
     CDS             complement(2638..3384)
                     /gene="ydfG"
                     /locus_tag="ACHXWD_003647"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR7622446.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3521..5566
                     /gene="dcp"
                     /locus_tag="ACHXWD_003648"
     CDS             3521..5566
                     /gene="dcp"
                     /locus_tag="ACHXWD_003648"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR7622447.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5611..6129)
                     /locus_tag="ACHXWD_003649"
     CDS             complement(5611..6129)
                     /locus_tag="ACHXWD_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR7622448.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1530 a         1484 c         1596 g         1574 t
ORIGIN      
        1 gttatcagca aaagcgacac tccctgttta tgatcgtaat aacctggccc caagaattat
       61 tcatttaggc tttggtgcat ttcaccgtgc gcatcagggc gtgtatgccg atattcttgc
      121 tacagaacat ttcagtgact ggggatatta tgaggtcaac ttaatcggcg gcgaacagca
      181 aattgccgat ttacaacagc aagataatct ttataccgtt gcggaaatgt cggccgatgc
      241 gtggacggct cgcgtcgttg gcgtcgttaa gaaagccttg cacgtacaga tggatggctt
      301 agaaaccgtg ttggctgcga tgtgtgaacc gcaaatcgcg attgtctctc tgacaatcac
      361 cgaaaaaggg tatttccact ctccggcgac cggacagtta atgctcgatc acccgatggt
      421 cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca acaggggtga ttgttgaggc
      481 gctggcccgc cgtaaagcgg caggacttcc cgcatttacc gtcatgtcat gtgacaacat
      541 gccagaaaac ggtcatgtta tgcgtgacgt tgtcacttca tacgcgaaag ctgttgatgt
      601 aaaactggca caatggatcg aagataacgt gactttccca tcaacaatgg tggaccgtat
      661 tgtgcccgca gtgacagagg atacgctggc gaaaatcgaa caacttaccg gtgtgggcga
      721 tcctgcgggc gttgcctgtg aacctttccg ccagtgggta atagaagata actttgttgc
      781 cggacgtcca gaatgggaaa aagcgggagc cgaactggtt agtgatgtgc tgccttatga
      841 agagatgaag ttgcgcatgc tcaacggcag tcattcattc ctggcgtatt tgggttatct
      901 tgctggatat cagcacatta atgactgcat ggaagatgaa cattatcgtt atgcggcgta
      961 tggcttgatg ttgcaggaac aagcgccgac gctgaaagtg cagggcgttg atttgcaaga
     1021 ttacgctaac cgattaattg aacgctatag caacccggcg ttacgtcatc gaacctggca
     1081 gattgcgatg gatggcagcc agaaattgcc acagcggatg ttggattctg ttcgctggca
     1141 tctggcgcat gacagcaagt tcgatctgct ggcactgggc gtcgcgggtt ggatgcgtta
     1201 tgtcggtggt gttgatgaac agggaaatcc gattgaaatc agtgacccac tgttacctgt
     1261 tattcagaag gctgtacaaa gtagtgctga agggaaagcg cgcgtccagt cattgctggc
     1321 gattaaggcg atctttggtg atgatttgcc agacaatagt ttgtttactg caaaagtgac
     1381 ggaagcgtac ttgtctttat tagcgcatgg cgcgaaagcg accgtggcga aatattccgt
     1441 gaagtaaaaa aagcaatgcc gccatcggcg gcattcacac gcttagttca tgccgagacg
     1501 aatcaggtcc agaggtgcca gtttctcttc acaaccttca acaactacgg tttttccgcg
     1561 gcggatttgc tcagcggtca gaccttcagt atcaatcgac aggatcttgc cagtgtgacc
     1621 ggtgccgcta accataacac ggctgccagt ggtgattgcg ttacggttac gatcgtaggt
     1681 cgtcataggt attttctcct ttctgattta cagtcgaagc ccacgtaacg ggcgcaatat
     1741 aaatactcct gtcgagtgaa atattttttg tttttgatca agctcacatc ttttttgata
     1801 catgcatgag ccataaggcg ggggaaaaga ctctcacccc ggatgggatg agaggaaatg
     1861 aattactctt cgctaaacca gtcgctattt tcctggcgga tctggcgcac ggattcgctg
     1921 atttcctgca aatgttgcgt cattgcacgt tctaccgcat cgccatcacg tttttgcagg
     1981 gcagagaaaa tatcaagatg ctggcgtaac agcatttctg gtggagaaac gtggtcgaaa
     2041 ctcatatagc gcacgcgatc aacggtcgct ttcaggttct caatggtatc ccacgccagt
     2101 tgacagtcgg caatctgcgt caggagttga tggaagttgt catcaagttc aaaaaaatca
     2161 tccagttgct tgcgctcaat ggcaatgcgt tgctggtgaa gattttgttc cagttggtag
     2221 cactggcttt cggtaatcat gctcgccgcc cgacgcgcca ccgcgcactc aatggcctga
     2281 cggataaaac tgccattgcg cacctgggcc atggaaattt tgttgacgta gctgccacgc
     2341 tgcggacgaa tttgaatcag gccgttttcc gccagtttaa taaaggcttc acgaaccggc
     2401 tggcgtgaca cattgaaacg aacagaaact tctttttccg acaacggtgt gcctggtgca
     2461 atcaggcaat ggacaatgtc gcgacgaaga atacgataaa tttgctgatt gacaggctgt
     2521 gtgggattaa gttgcgtttc gacggtcatt cgttcttgct tgtgagtgag ttaactgcat
     2581 gagtctacca cttttttgtg acaagcaata acccggcagt tacgccgggt ataaaaatta
     2641 ctgacggtgg acattcagtc cggcatagct ttgggtaacc ggcatcattt ccagggtatt
     2701 gatattgacg tgagcaggca gcgttgacac ccaccagacg gcttcgctga catcttctgg
     2761 cgtcaatgca acggtatttt gataggtttt ttccgcttta ccgtcatcgc ctttaaagcg
     2821 gacattggaa aactcggtgc cacccaccag acccggttcg atgtcggtga cgcgcaccgc
     2881 cgtaccatgc aggtccgtac gcagattcag gctaaactga cgaacaaacg ctttcgtcgc
     2941 accgtaaacg ttaccaccgg catacggcca gctacctgcc gttgagccaa tgttaataat
     3001 atgaccatga ttacgttcaa ccatacccgg taagacggcg cgcgtcatat ataccaggcc
     3061 tttgttgttg gtatcaatca tcgtttccca gtcttcaacg ctggctttat gcgcaggctc
     3121 catgcccaac gccaggccgg cattatttac caggatatca atattgcacc actcggcagg
     3181 aagcgatgcc agcatctctt caatagcggc gcggttgcga acgtccagtt gggcgatata
     3241 cagattatct cccagttcgt cttttaactc ctgcaaccgc tcctggcgac ggccagtggc
     3301 gataacttta tgcccttgtt gaataaaacg acgagtaatg cattcaccaa aacctgccgt
     3361 tgctccagtt actaaaacga tcatctcact gttcctcaac gcttttgtgt gtcaataaca
     3421 tagcacgtga tgcttaagag cgggtaatac tggtttgcaa gattgcactc cgttatcagt
     3481 aagcctacgc tatggaacat ctcgcgttca ggagaagaaa atgacaacaa tgaatccttt
     3541 ccttgtgcaa agcacactac cgtatctggc tccccatttt gatcaaattg ccaatcatca
     3601 ctatcgcccg gcattcgatg agggaatgca gcaaaagcgg gcagaaattg ctgccatcgc
     3661 gcttaacccg caaacgcctg atttcaacaa tactattctg gcactggaac aaagcggaga
     3721 attacttacc cgcgttacca gcgtcttttt tgcgatgact gcggcgcata ccaatgatga
     3781 attacagcgt cttgatgaac agttttccgc tgaactggcg gaactggcta atgatatcta
     3841 tctgaacggt gaattattcg cgcgggtaga tgctgtctgg cagcgccgtg aatccctggg
     3901 gcttgatagt gaatccatcc gcctggtgga ggtgattcat caacgttttg tccttgccgg
     3961 agccaaactt gcgcaagctg ataaagcaaa attaaaagta ctgaatacag aagctgcgac
     4021 cctgaccagc cagtttaacc agcgattact ggcagcaaat aaatccggcg gtctggttgt
     4081 gaacgatatc gcgcagctgg caggaatgag tgagcaagag attgcgctgg cggcagaggc
     4141 ggctcgcgag aaaggtctgg ataacaaatg gctgattccg ctgctgaata ccacccaaca
     4201 accggcgctt gccgaaatgc gcgatcgtgc gacgcgtgaa aaactgttta ttgcgggctg
     4261 gacgcgagcg gaaaaaaatg atggcaatga tacccgcgct atcattcaac gtctggtaga
     4321 gattcgcgca cagcaggcga aactgcttgg ttttcctcat tatgccgcat ggaaaatcgc
     4381 cgatcagatg gcaaaaacgc cagaagcagc actcaacttt atgcgggaaa ttgttccagc
     4441 ggcgcgtcaa cgtgcgagcg atgaattagc ctccatacag gcggttatcg ataagcagca
     4501 gggcgggttt agcgcgcagc cgtgggactg ggcattttat gcggaacagg tacggcggga
     4561 gaaatttgat cttgatgaag cgcagctcaa gccatatttt gaattaaaca cggtgttgaa
     4621 tgaaggtgta ttctggaccg cgaatcagct cttcggtatt aagtttgtcg aacgttttga
     4681 tattcctgtc taccatcctg acgttcgggt gtgggaaatt tttgatcata atggcgtggg
     4741 gctggcgtta ttttacggtg atttcttcgc ccgtgattca aaaagcggcg gtgcatggat
     4801 gggcaatttt gttgagcaat caacgcttaa tgaaacgcat ccggtaattt ataacgtctg
     4861 caattatcag aaacccgctg ccggtgagcc tgcgttgtta ctctgggatg atgtaataac
     4921 cttattccat gaatttggtc atacgctgca cggccttttt gcccgccagc gttatgccac
     4981 gctttccggc accaacacgc cgcgtgattt tgtcgaattt ccgtcgcaaa tcaacgaaca
     5041 ctgggcaacg catccgcagg tattcgctcg ctacgcccgg cattatcaga gcggggcagc
     5101 aatgcctgac gaactgcaac agaaaatgcg taatgccagc ctgttcaaca aagggtatga
     5161 gatgagcgaa ctgcttagcg ccgcacttct cgatatgcgc tggcattgcc tggaagaaaa
     5221 cgaagcaatg caggatgtcg atgattttga attgcgggcg ctggtggcgg aaaatatgga
     5281 tcttcctgct ataccgccac gctatcgcag cagttatttc gcccatattt ttggtggcgg
     5341 atatgctgca ggttattacg cttatctgtg gacgcaaatg ttggccgatg atggttacca
     5401 gtggtttgtt gagcagggcg gattaacgcg tgaaaatggg cagcgttttc gcgaggcgat
     5461 cctttccaga ggtaacagcg aagatctgga acgcctgcat cgacaatggc gcggtaaggc
     5521 tcctcagatt atgccgatgc tgcaacatcg tggcttgaac atataaacac caccaatggc
     5581 tggcctgggg atatcgccag gccagcctgg ttatagcagt aattgcagca gctgggcgag
     5641 ggcaaacctc accgctaaag ctaataccgt ttcgcagtcg ccagcaaaat gcataaccgc
     5701 agtgtagttc tggcctttaa tatgccacgc gaaccagacg gtacccgctg gcgtaccatc
     5761 ttcaccgccc tccggtccgc cgtagccggt aatggcaata ctgacatcag catccgcacg
     5821 ctctatggca cctgttgcca tttctgctgc cactttctca ctcaccgcag aatatcgttc
     5881 aagagactgc tggcttacgc tgaggatttt catctttgcc tgatcggtga aagtaacaaa
     5941 gcctgcaccg taaaatttgg gtgtatcttc agctgcacac agggcgctag ccaacttacc
     6001 gccggtgcat gattcggcgg tagttaagcg taattgatcc gcgataagtc tttgactcaa
     6061 cgcggatgtc aggttttcaa tagtgtcggt atctgcgagt tgtacaattt tatctttatt
     6121 aatgttcact gttttttgtc cacgctatta tatgtaaagg tgatttgctt tttctgtatg
     6181 ctcg
//
LOCUS       DAZSBF010000232         6136 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZSBF010000232 DAZSBF010000000
VERSION     DAZSBF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.237"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            66..842
                     /gene="exuR"
                     /locus_tag="ACHXWD_003650"
     CDS             66..842
                     /gene="exuR"
                     /locus_tag="ACHXWD_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR7622449.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1082..1165
                     /gene="yqiM"
                     /locus_tag="ACHXWD_003651"
     CDS             1082..1165
                     /gene="yqiM"
                     /locus_tag="ACHXWD_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR7622450.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1187..1849
                     /gene="yqjA"
                     /locus_tag="ACHXWD_003652"
     CDS             1187..1849
                     /gene="yqjA"
                     /locus_tag="ACHXWD_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR7622451.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1853..2236
                     /gene="mzrA"
                     /locus_tag="ACHXWD_003653"
     CDS             1853..2236
                     /gene="mzrA"
                     /locus_tag="ACHXWD_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR7622452.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2383..2751
                     /gene="yqjC"
                     /locus_tag="ACHXWD_003654"
     CDS             2383..2751
                     /gene="yqjC"
                     /locus_tag="ACHXWD_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR7622453.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2789..3094
                     /locus_tag="ACHXWD_003655"
     CDS             2789..3094
                     /locus_tag="ACHXWD_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR7622454.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3097..3501
                     /locus_tag="ACHXWD_003656"
     CDS             3097..3501
                     /locus_tag="ACHXWD_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR7622455.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3491..3790
                     /locus_tag="ACHXWD_003657"
     CDS             3491..3790
                     /locus_tag="ACHXWD_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR7622456.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3976..4368
                     /locus_tag="ACHXWD_003658"
     CDS             3976..4368
                     /locus_tag="ACHXWD_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR7622457.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4438..5423
                     /gene="yqjG"
                     /locus_tag="ACHXWD_003659"
                     /pseudo
     CDS             4438..5423
                     /gene="yqjG"
                     /locus_tag="ACHXWD_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5716..6081
                     /locus_tag="ACHXWD_003660"
     CDS             5716..6081
                     /locus_tag="ACHXWD_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR7622458.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1534 a         1514 c         1546 g         1542 t
ORIGIN      
        1 caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca ctccgggaga
       61 cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgccgct gacctgaaag
      121 agcgcattga acagggcgtc tatctggtgg gtgataaact gcctgccgaa cgctttattg
      181 ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg ctggaagttg
      241 aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac cagccgcgcc
      301 atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt gagttgcttc
      361 aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag gtaacgaaac
      421 aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa tgcttccgtg
      481 attccgagtg ggatttacag ttccacattc aggtagccct ggcgacgcag aactccgccc
      541 tggcggccat cgttgaaaaa atgtggaccc agcgtagcca taacccgtac tggaaaaaac
      601 tacacgaaca cattgattcc cgtaccgttg ataactggtg tgatgaccac gatcaaattc
      661 tcaaggcgtt aattcgcaaa gatccccatg ctgctaagct ggcaatgtgg cagcatctgg
      721 aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga tttcgagttc aatgccgacc
      781 gctatctatt cgccgaaaac ccggtagtgc atctcgatac ggccaccagt ggcagtaaat
      841 gaaactcctg tccgacaggc gctgcgacgc gcctgttcgc ttcgtcggta agcgaaaagt
      901 ataaagtgtc agcctgtgta aatcctctcg ccaccctccc ctgcattcag caaaatcaga
      961 ctccacggac atgtaatttt gataacgaac aacgttgacc tttgttacaa ttagattcaa
     1021 tttgaattta tgtttttgaa tgctttctta tctcacgatt taacagggaa tagttcaggc
     1081 tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc aataacattg cggtagtgca
     1141 tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa taatgaatgg aacttttgac
     1201 ccaattgctg caagccctgt gggcgcagga ttttgaaacc ctggccaatc catcgatgat
     1261 tggcatgttg tattttgtct tgtttgtaat tttgttcctt gaaaacggct tgcttccagc
     1321 ggccttttta ccgggcgaca gtttactggt attggtcggc gtgttgattg cgaaaggcgc
     1381 gatgggttac ccgcaaacga ttctgctgct gaccgttgcc gccagcctcg gctgctgggt
     1441 cagctatatt caggggcgat ggctgggcaa tacccgcacc gtacaaaact ggctatctca
     1501 tttacccgcg cattatcatc aacgcgcaca ccatcttttt cataaacacg gtttatcggc
     1561 gctgttaatt ggtcgcttta ttgcgtttgt cagaacactg ctgccgacga ttgccgggtt
     1621 atcagggctg aataacgcgc gctttcagtt tttcaactgg atgagcggtc tgctgtgggt
     1681 attgatcctg acaactctgg gttacatgct cggcaaaacg ccgatatttt taaagtacga
     1741 ggaccagctg atgtcatgcc tgatgctgct cccggtggtg ctgctggtgt ttggcctggc
     1801 aggttctctg gtcgtgttat ggaaaaagaa atatggaaat cgggggtaag ggatgcaaat
     1861 acctcgcatg tcgcttcgcc agctagcctg gtccggcact gttttacttc tggtcggcac
     1921 gctgttgctg gcctggtccg cggttcgcca gcaagagtct acgctggcga ttcgtgccgt
     1981 tcatcaaggc acaacgatgc cagacggttt ttcgatctgg catcaccttg acgctcatgg
     2041 cattcctttc aaaagtatca cccccaaaaa cgacactctg ttaattacat ttgactccag
     2101 cgaccagagc gccgccgcaa aagcggtcct cgacagaaca ttgccccatg gctacatcat
     2161 tgcgcagcag gacaataaca gtcaggctat gcagtggctg acccggttac gggataactc
     2221 tcatcgcttc ggataacttc ctgttatccg aaacatatca ctcactttgg tgatttcacc
     2281 gtaactgtct atgattaatg aggcggtggt taacactacc gcttcgttgt ctggacaatc
     2341 gttcctttgt aataggtccg aatcacaatg gaaggttcaa gaatgaaata ccgcatcgct
     2401 ttagctgttt ctctctttgc tcttagtgcc ggtagttatg ccactaccct gtgtcaggaa
     2461 aaggagcaaa atatccttaa ggagatcagc tatgccgaaa aacaccaaaa ccagaatcgt
     2521 attgacggtc tgaataaagc cctgagtgaa gtccgggcca actgttcaga tagccagctg
     2581 cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg aggtggcgga acgccagcaa
     2641 gatttagccg aggcgaagca aaaaggcgat gccgataaga ttgccaaacg cgaacggaaa
     2701 ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc gcgactacta actcacaata
     2761 gtcactactt actcacctgg agaaaactat gtcgaaagaa cacactacgg aacatctgcg
     2821 tgctgagttg aaatcccttt ccgatacgct ggaagaggtg cttagctcat ctggcgagaa
     2881 gtcgaaagaa gagttgagta agattcgtag caaagcggag caggcactga aacagagccg
     2941 ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa acccgtgtcg cggcggcgcg
     3001 tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg ggaattggcg ctgcaatcgg
     3061 tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg cggacactca tcacgcgcaa
     3121 gggcccggta aaagcgttct gggcatcggg cagcgaattg tttccatcat ggttgaaatg
     3181 gtggaaacac gtctgcggct ggcggtggtg gagctggaag aggaaaaagc taatctcttt
     3241 caacttttac tgatgctggg cctgacgatg cttttcgctg catttggtct tatgagcctg
     3301 atggtgctaa ttatttgggc ggttgatccg caatatcgcc tgaatgcgat gattgccacc
     3361 accgtggtgt tgctgctact ggcactgatt ggcggtatct ggacgttacg taaatcgcgt
     3421 aaatccacct tgctgcgcca tacacgccat gagctggcaa acgatcggca actgctcgag
     3481 gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt aaggcgcaac tgcttagcca
     3541 gatccagcaa caacggctgg atctttccgc cagtcgtcgt gaatggctgg aggcaacagg
     3601 cgcttacgat cgtcgctgga atatgctgct aagtctgcgc tcctgggcgc tggttggcag
     3661 tagcgtgatg gcgatctgga cgattcgcca tcctaatatg ctggtccgct gggccagacg
     3721 cggttttggc gtatggagcg cctggcgtct ggttaaaacg accctcaagc agcaacagct
     3781 tcgcggttaa cttccctctg gccggagcaa ttccggcctt atccctcaaa ttttttgaag
     3841 atttttgaca gttttccttg ctaacaatca tcattcacca cgtttatgat tctctccatc
     3901 gacagcaacg acgctaatac cgcgcccttg cacaaaaaaa caatcagcag cctgagtggc
     3961 gcagtggaga gtatgatgaa aaaattagaa gatgttggtg tactggtagc gcgcatttta
     4021 atgccgattc tgtttattac cgcaggctgg ggaaaaatca ccgcttactc tggtacccaa
     4081 caatatatgg aagcaatggg tgtcccgggt tttatgctgc cgctggtgat tctgcttgag
     4141 ttcggtggtg gtctggcaat cctgttcggt ttcctgactc gcaccacagc cctgtttact
     4201 gcgggcttta cgctgctgac ggcattttta tttcacagca actttgctga aggcgtcaac
     4261 tcgctgatgt tcatgaaaaa cctgacaatt tctggcggat tcctgctgct ggcaattacc
     4321 ggtccgggcg cgtatagcat cgaccgcctg ctgaataaaa agtggtaagc acgcgctata
     4381 cttaacgata aaaaacgagg aggaagctcc tcgtttttgc tattggagga gagaaaaatg
     4441 ggtcaactga ttgacggcgt ctggcatgac acctggtacg ataccaaatc taccggcggt
     4501 aaatttcaac gttcagcttc cgcatttcgt aactggctca ctgccgatgg cgcacctggc
     4561 cccactggca caggcggttt tatcgcagag aaagatcgtt atcatctcta tatttcactc
     4621 gcctgcccgt gggcgcaccg cacgctgatc atgcgcaaac tcaaaggact ggaaccgttt
     4681 atttccgttt ccgtagtgaa cccgctgatg ctggaaaacg gctggacctt tgatgacagt
     4741 tttccgggag caaccggaga tacgctctat caacatgaat ttctgtatca gctttatctc
     4801 cacgccgatc cacactacag cggacgagtt actgttcccg tactgtggga caaaaagaac
     4861 cacaccatcg tcagcaacga atcaacagaa atcatccgca tgttcaatac cgcgtttgat
     4921 gcgctgggcg cgaaagcggg tgattactac ccaccagccc tgcaaacgaa aattgacgaa
     4981 cttaacggct ggatttatga caccgttaac aacggcgtgt ataaagctgg ttttgccacc
     5041 agccagcaag cctacgacga ggcggtggcg aaagtgtttg aatcgctggc gcgactggag
     5101 cagattttag gccagcatcg ttacctgacc ggcaaccagc taaccgaagc cgatattcgc
     5161 ctgtggacta cgctggtgcg ttttgatcca gtgtatgtga cccacttcaa gtgtgataag
     5221 caccgcatca gcgattacct gaatctgtat ggcttcctgc gcgatatcta ccagatgccg
     5281 gggatcgccg aaactgtcaa tttcgatcac atccgtaatc attacttccg cagccataag
     5341 accatcaacc ctacggggat tatttcaatt ggtccgtggc aggatctcga tgaaccgcag
     5401 gacgagatgt tcgcttcggt taaaaaaagg caccttacga agtgcctctg ataattagct
     5461 aagtgcatgt tgttcatatc ggatgcggcg tgaacgcctt atcaaaccta caaaacattt
     5521 atattcaata tgttgcaatg accgcgcagg ccagataagc gtagcgcatc tggcgatttt
     5581 gcgcttgtca tcagtcttag cgcgccttca cagcacttta ttaattcctt gaacgaatat
     5641 ttactgccat ttcattcacg tctattctta atttgctgct ttaaaaacaa gtgattgagc
     5701 aacattgagg caaaaatgga ctggtatctg aaagtaataa aaaattatgt cggtttccgg
     5761 ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg tcaacatcat ctttacgttc
     5821 gtgctggggc tgctggataa aatgttaggc tggcaacgcg ctggtggcga aggcatcctg
     5881 acgacaatct acggtattct ggtgttttta ccgtggtggg cagttcagtt ccgccgcctg
     5941 cacgacaccg accgctcggc gtggtgggca ctgctattct taatcccgtt tatcggctgg
     6001 cttattatca tcgtctttaa ctgccaggcg ggtacgccag gcgaaaaccg ctttggacct
     6061 gatccaaaac tggaacctta aaacaaatct gctcgccgga tgtctgactc atccggcttt
     6121 tatcatcatg ttgatg
//
LOCUS       DAZSBF010000233         6120 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZSBF010000233 DAZSBF010000000
VERSION     DAZSBF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.238"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHXWD_003661"
     CDS             <1..358
                     /locus_tag="ACHXWD_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR7622459.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHXWD_003662"
     CDS             complement(398..1069)
                     /locus_tag="ACHXWD_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR7622460.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHXWD_003663"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHXWD_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR7622461.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHXWD_003664"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHXWD_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR7622462.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHXWD_003665"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHXWD_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR7622463.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHXWD_003666"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHXWD_003666"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR7622464.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHXWD_003667"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHXWD_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR7622465.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHXWD_003668"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHXWD_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR7622466.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="ACHXWD_003669"
     CDS             complement(5600..>6120)
                     /gene="fliD"
                     /locus_tag="ACHXWD_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR7622467.1"
                     /translation="KEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAFK
                     TLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKGY
                     LADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTST
                     YLSQQFTAMSNS"
BASE COUNT         1497 a         1608 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
//
LOCUS       DAZSBF010000234         6063 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZSBF010000234 DAZSBF010000000
VERSION     DAZSBF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6063
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.239"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHXWD_003670"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHXWD_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR7622468.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHXWD_003671"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHXWD_003671"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR7622469.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHXWD_003672"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHXWD_003672"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR7622470.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHXWD_003673"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHXWD_003673"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR7622471.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHXWD_003674"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHXWD_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR7622472.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1437 a         1530 c         1646 g         1450 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaat atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 cta
//
LOCUS       DAZSBF010000235         6020 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZSBF010000235 DAZSBF010000000
VERSION     DAZSBF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.240"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..394)
                     /locus_tag="ACHXWD_003675"
     CDS             complement(<1..394)
                     /locus_tag="ACHXWD_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7622473.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKA"
     gene            981..1328
                     /locus_tag="ACHXWD_003676"
                     /pseudo
     CDS             981..1328
                     /locus_tag="ACHXWD_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1381..1776
                     /locus_tag="ACHXWD_003677"
     CDS             1381..1776
                     /locus_tag="ACHXWD_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR7622474.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1817..2560
                     /gene="cmoA"
                     /locus_tag="ACHXWD_003678"
     CDS             1817..2560
                     /gene="cmoA"
                     /locus_tag="ACHXWD_003678"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR7622475.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2557..3528
                     /gene="cmoB"
                     /locus_tag="ACHXWD_003679"
     CDS             2557..3528
                     /gene="cmoB"
                     /locus_tag="ACHXWD_003679"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR7622476.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3693..>6020)
                     /gene="torZ"
                     /locus_tag="ACHXWD_003680"
     CDS             complement(3693..>6020)
                     /gene="torZ"
                     /locus_tag="ACHXWD_003680"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR7622477.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1407 a         1542 c         1414 g         1657 t
ORIGIN      
        1 cagcctttct acgctctgct atcaagcttc tttcaagctg tgccaacgtt gacataatgc
       61 ctaacattgc ttcacccata gcgcttgata agtcaaatcc ttctctaagg ctgataactg
      121 tcacaccttt agcctgtagc gctttaaccg ttgataacac atcaattgtg tcacgtccaa
      181 gacggtctac agctaccaca actacggtgt caccttctcg tgcataagct aacaaactgc
      241 tgaaaccgtt tcttaaagaa gctttgacag aaccactgac agcttcatca gcgaaccatt
      301 tagcaatgtt gtaccctgcc tcttcaattt gcatttgctg gttagtgatg gattggtcat
      361 ctgtactcac cctgcaatat ccaagtatcg ccataatgtt ccccttagaa gtctcaaaag
      421 ttagtctcat aagcttagct gtctcataat ccaaaaacaa ccttttgaga ctacttgagg
      481 gtgcattcta ccgttacgtt gaaagtgtct caaaaggact tccttatgag actggttaat
      541 agagcgctat gaagattcag gagggtaact ataaccatca ctttctaact ttcacgtagg
      601 aaatcgttaa gtttcttgat agttacacca tgtgaccaac tatctatata tcgttatata
      661 gccctgtata tagcactata tgtagtgtct ggagagggac gactaacacg atacagttta
      721 ccctgaaaag agacttcaca gattctttat atatttcaat aagttatgct aaaaagagac
      781 tataccgata ctgcatagaa tttgtgtagg attgcataga tacttggaaa aacaccgtta
      841 aattgcatag tcattcacct gatattgtcc tgtttttaca ggtagttgat tcggatgttc
      901 taggaatcat cctcatagtt gattcggtta gttgggcttg taccaacccc taatcaacct
      961 catagtgtat ttagaccttg atgattttag acagccgccc tgttcatgca gcacgtccac
     1021 acagtgaagc tattcgcgac gctcaacgaa aaaaacctaa agttccggta catgctgtac
     1081 tgacggcgac aaatccgctg attcgtttta tcggtagtga tgatatgacg caaaaccggg
     1141 aattatttca ggtctggtta cagaaattag cgcagtggca tcagaccact acgccttatc
     1201 tttttttaca tacgccagac attgcccagg ccccggaact ggtacatacc ctgtgggaag
     1261 acttacgtaa aacgcttcca gagatcggag cagttccggc tattccacag caatcttctc
     1321 ttttctgaat ttgccaccta tcatagacag gtgccatcgg ccattttaaa gggagtttgt
     1381 atggtaagcg cgctatatgc cgttttaagt gcgttgttat taatgaagtt ctcttttgat
     1441 gtcgttcgcc tgcgaatgca gtaccgcgtt gcctatggtg acggtggttt tagcgaactg
     1501 caaagcgcta ttcgcattca tggtaacgcg gtggaatata ttcccatcgc gattgtgctg
     1561 atgctgttta tggaaatgaa tggcgcagaa acctggatgg tgcatatttg cggcatcgtt
     1621 ttgcttgctg gtcgtctgat gcattattac ggttttcatc accgtctgtt tcgctggcga
     1681 cgttccggca tgagcgccac ctggtgtgcg ctgttgctga tggtgctggc gaatctttgg
     1741 tatatgccct gggagttggt tttctccctg cgttagcgca caatacgcca ctttcttttt
     1801 cccggatttt tacgttatgt ctcaccgcga cacgctattt tctgccccta tcgccagact
     1861 gggcgactgg acctttgatg aacgggtagc tgaagtcttc ccggatatga tccagcgttc
     1921 cgttcccggc tattccaata ttatttccat gattggtatg ttagccgagc gcttcgttca
     1981 acctggtacg caggtttacg atctgggttg ttctctgggc gcggcgacgc tctcggtgcg
     2041 tcgcaacatt catcatgata attgcaaaat tattgccatc gacaactccc cggcgatgat
     2101 tgaacgctgc agtcgtcata ttgacgccta taaagcccct acgccagtag acgtcattga
     2161 aggtgatatt cgcgatatcg ccattgaaaa cgcatcgatg gtggtgctga attttaccct
     2221 gcaattcctg gaaccttccg agcgccaggc attactggat aaaatttatc aaggactgaa
     2281 cccgggcggc gcgctggtgc tttcggaaaa attcagtttc gaagatgcca aagttggtga
     2341 actgttgttc aacatgcacc acgactttaa acgtgccaac ggttacagcg aactggagat
     2401 cagccagaag cgcagcatgc tggaaaacgt gatgctgacc gattccgtgg aaacccataa
     2461 agcacgcctg cataaagccg gttttgagca tagcgagctg tggttccagt gctttaactt
     2521 tggttcactg gtggcattaa aagcagagga cgctgcatga tcgactttgg taacttttat
     2581 tctctgattg ccaaaaatca tctttcacac tggctcgaaa cgctgcccgc gcagattgct
     2641 aactggcagc gcgagcagca gcacgggctg tttaagcagt ggtccaacgc ggtggaattt
     2701 ctgcctgaaa ttaaaccgta tcgtctggat ttattgcata gcgtaaccgc cgaaagcgaa
     2761 gagccactga gcaccgggca aattaaacgc attgaaacgc ttatgcggaa cctgatgcca
     2821 tggcgcaaag ggccgttctc actgtatggc gtcaacatcg ataccgaatg gcgttccgac
     2881 tggaaatggg atcgtgttct gccccatctt tctgatttaa ccggacgcac cattctggat
     2941 gtcggctgcg gcagcggtta tcacatgtgg cgcatgattg gcgcaggcgc gcacctcgcg
     3001 gtggggatcg accctacgca actgtttctg tgccagtttg aagcggtgcg taaactactg
     3061 ggtaacgatc agcgcgcaca tttgttaccg ttaggtattg aacagcttcc ggcactgaaa
     3121 gcctttgata ccgtcttttc gatgggcgtg ctttaccatc gccgttcgcc gctggagcat
     3181 ctctggcagt tgaaagatca actggtgaat gagggtgaac tggtgctgga aacgctggtt
     3241 attgatggcg atgagaacac ggtgctggta ccaggcgatc gttacgcgca aatgcgtaat
     3301 gtctatttca tcccttccgc gctggcgctg aaaaactggc tgaagaagtg tggttttgta
     3361 gatatccgca ttgtggatgt gtgcgttacc actactgaag agcagcgacg cactgaatgg
     3421 atggttaccg aatctctgtc cgatttcctc gacccacatg atccgagtaa gacagttgaa
     3481 ggttatcctg cgcctaaacg cgcggtgctg attgcgcgca agccgtaaag gtctggtatt
     3541 actgccggat gcggcgtgaa tgccttatcc ggcctacaaa cccttgctaa ttcaatctat
     3601 tgcagggact atgtaggcct gataagcata gcgcatcagg cagttttacg tttgcataac
     3661 ctcagcgccc gtttccgggc gctattcact tcttacgcct gaacagcagg cttatcaaat
     3721 gccgttaact tcggtgcgtt acccgtatat ttttcgatat acaccagcgc agagttaccg
     3781 gcacaggcat ttgccagctg cgagctgggg atatccgccg ttaacacgtt cgcactgccg
     3841 tttttacaca agccgttttc cagatctggc catgcacctt catgcacgca taccacgcct
     3901 tttttgatcc cgtcagtcac caccgcgcct gtcaaaatct gaccacgttt gttccagaca
     3961 cgcaccagat cgccattcgc aatactaaat cgagcagcat cttcggtgtg aatagtgatc
     4021 ggttcacgat cggcaaccgc atatttttta cgcagttccg catagttaag ctgactgtgt
     4081 aaacggtgtg ccggatgtgc ggtcagaagc tgcaactgct tctcgtcggc ggtacccttc
     4141 cactcatcag gcgcaagcca ggttgggtgt gccgggcaat ccttatagcc aaatttttcc
     4201 agcgttttgg agtaaatctc aattttgcca cttggcgtac ccagcgcatt tttcaccgga
     4261 tcggcgcgga aatcaccata acgaacgtac tgttcgttct tctcgctgcg gcgcatttcg
     4321 atcagtttat tttgctgcca gaaggcatta aacattggca tagtgacgcg ttgcgcacgg
     4381 gcccctttct gagcggcatc atagaaaaat ttcagccacg ccatttcatc tttaccttcg
     4441 gtatagatct cttttccgcc aggtttgagt aattccgcaa gatcggcaaa cacatcaaaa
     4501 tcgtttcgcg cttcaaattg cggagcaaca gcctgcttca tcggcacaat atgctggttg
     4561 ctgtaatcac cggtcatcgt caagtcattg cgctcaaacg atgtggtgat cggtaatacg
     4621 atatctgcgt gtttagcagc tgcggtccag tagcattcag aaacgacgat catctccggt
     4681 ttctgccatg ctttaatcag acgattagta tcctggtgat gggtaaagtt accgccaccc
     4741 gcccaccaga tcattttgat attcggatac gtttgctctt taccgttatg ctgatatttg
     4801 ccgccaggat tttccagcgc atcgacgata cgggcaacag gaatcgccct cattccgcca
     4861 tcatcagcgg cctcgctggc ctgtccggca atcgccgcag acatttccgg cagcacgccg
     4921 ccaacacgtg tcgggttgcc accattggag taatgataag agaaaccaaa accgccgccc
     4981 ggtgtaccaa tctgccctaa catcgccgcc agcgtcacca gcatccagtg tttctgttca
     5041 ccatattgct ggcgctgaat tccccagcct gccatcagca ttgtgcgatt cgccgccatc
     5101 agttcagcga gtttgactat ctgcgcttcc ggtacgccag taatttccgc cgcccaggcc
     5161 gcgctcttcg gcgtattatc gctcttacct ctcagatact cttcaaactg cggatacccg
     5221 gtagtgtatt tttcaaggaa cactttatcg tgtttgcctt gtgtcatcag ggtatgcgca
     5281 atccctaaca tcagtgccac gtcggtgccc atattcggcg cgatccaggt ggcattatcg
     5341 ccaaagaagt cgatggtttc ggagcggata ggatcaatgg caatcactgg tttgccagat
     5401 tttttcagct gatggaagta ttccagccct tgctcatcgg tactgctcca ggcaattttt
     5461 aaggtattca gcgggttcat cccccacagc accacaacct ggctgttttc cagaatcagc
     5521 ggccaggagg tctgctgttc atacacctct acagaaccga ccacatgcgg catgatcacc
     5581 tgtgccgccc cggttgaata atcgccgcta tgcccggaat aaccgcccgc caggttcata
     5641 taacgttgca gtaaggtttg cgctttatgc aacacgccag aagagcgcca gccgtatgat
     5701 ccggcaaaaa tagccgatgg tccgttagct ttgcgaatgc gatcgtgttg ctcatgaatc
     5761 agttttaatg cttgttccca gctcacctgt acataggtat cttcgccacg acctttcgtc
     5821 ggttgcagtg gattatcgag atagcttttt ctcaccatcg gatgctgaat acgcgtcgtg
     5881 gtgtgtacct gatccgccgc cgtagactgt aaggaattcg gtatggtttt cgccagcgcg
     5941 cctgttgaag aaacaatctt gccgtccttc acttctacgt tcatcgctcc ccaacgttcc
     6001 gcggtgagga ttttaccgtc
//
LOCUS       DAZSBF010000236         6015 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZSBF010000236 DAZSBF010000000
VERSION     DAZSBF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6015
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.241"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(369..1304)
                     /locus_tag="ACHXWD_003681"
     CDS             complement(369..1304)
                     /locus_tag="ACHXWD_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622478.1"
                     /translation="MSAALQYFEENLPLRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDLNAPAPNITIKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     TSARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTAETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
     gene            complement(1802..1945)
                     /locus_tag="ACHXWD_003682"
     CDS             complement(1802..1945)
                     /locus_tag="ACHXWD_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFR7622479.1"
                     /translation="MKKITGIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGV
                     ETQ"
     gene            complement(2029..2286)
                     /locus_tag="ACHXWD_003683"
     CDS             complement(2029..2286)
                     /locus_tag="ACHXWD_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="HFR7622480.1"
                     /translation="MGLKLHIHWFDKKNEEFKGGEYSKDFGDDGSVIERLGMPLKDNI
                     NNGWFDVEKRWVPILQPYFKNVIDISKFDYFVSFDYRDGDW"
     gene            complement(2296..3933)
                     /locus_tag="ACHXWD_003684"
     CDS             complement(2296..3933)
                     /locus_tag="ACHXWD_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase
                     domain-containing protein"
                     /protein_id="HFR7622481.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVS
                     GEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTN
                     VQVSTLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASF
                     PGVPSLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKP
                     VYVSVTDVLTPAQVKQRQDEEKRLQQEWNDAHPVEVAERNYEQARAELNQANKDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQ
                     RAQTDVNNKKAAFDAAAKEKSAADAALSSAMESRKKKEDKKRSAENKLNEEKNKRRKG
                     TKDYGHDYHPAPKTEDIKGLGELKEGRPKTPKQGGGGKRARWYGDKGRKIYEWDSQHG
                     ELEGYRASDGQHLGSFDPKTGKQLKGPDPKRSIKKYL"
     gene            complement(4154..4687)
                     /locus_tag="ACHXWD_003685"
     CDS             complement(4154..4687)
                     /locus_tag="ACHXWD_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622482.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQRLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFSVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(4690..5616)
                     /locus_tag="ACHXWD_003686"
     CDS             complement(4690..5616)
                     /locus_tag="ACHXWD_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR7622483.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSG
                     SLTLTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDRVSRQNRTEPERVKSPSRGFSMGF"
     gene            5792..>6015
                     /locus_tag="ACHXWD_003687"
     CDS             5792..>6015
                     /locus_tag="ACHXWD_003687"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622484.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEG"
BASE COUNT         1487 a         1660 c         1297 g         1571 t
ORIGIN      
        1 aaaaaaacaa cgacgagaac gcaatcaccg actctttctc gcggctgggc tactgatcga
       61 agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca ggatattaca
      121 ggcacttaag gaaattaagt acgatctgga gacatcccca aacccagacg cgtaagtgcc
      181 tccatccgtg ttcctgttcc ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat
      241 gagactgcgg cgagcactac cagcacaatc atgcggaaaa cagcaccatt gcagggctga
      301 acggggaaaa acggcaaaag acctaaggct caaaaaaacg ccagaaaggc gctgttatct
      361 gatataggct attttccgct tttccagttt cgaatggttc gagtagaaac ctccaacatc
      421 gccgcaatag ctttctgaga atatccattc tccagcatac atagcgccat gaaacgcttg
      481 tcatcgtagg cttcgccttt agcgatgcca cctttcctgc ctctcttggc ctgtatttct
      541 ggcgtgtgcg tatcagccac atactgtgcg aaagtttccg ccgtgaagtt cctgtgcgtg
      601 tatttcgcaa tactcttgcc aatagcccga cattcaggag gtgaaagcgg aacgggaagc
      661 gatgcgttgt acatttcgac acgctgaatc acggcatcaa gccactgtga gaatgctggc
      721 cagccctgac gaatcgccct gtaagcccat ttgcgcgtca tttcgaacag atggcagttt
      781 ctccccattc cgtaatgttt atcgatgcta cggcgcgctg aagtgctcaa atcgagataa
      841 tcagccagtt catcgagggt ataggcttcc tcacgccact ccatcacctg ccattccaga
      901 tggaacggat ttttgcaaat cagtccgctg tagtttacat ctgcgcccag tttttcacac
      961 aacgcacgtt caatcgcggc ggcgtatttc agcgccttaa ccgaagcatc aggcgcggtt
     1021 ctcacggcga tattgagtgc gtagagcagg tgggcgtgac cgttcacggg attttttatg
     1081 gtgatgttcg gtgcgggggc gttcaggtcg ctccagtcaa tcgcagcccc ctcgcggtca
     1141 acgtcaaaaa ccagccagaa ctgcgcatga ggttggttct gctggatata ccgtgcaaga
     1201 agcgcgcgcc ctttaccgga gatacgaagg ccgaaagcga gatcatccgt gtgatagggg
     1261 cggaggggta aattttcttc gaagtattga agcgcggcgc tcatgtgtta cgattccttc
     1321 caggttatcg accgacctcg cccctgtatt ctcgtgacaa tcttggcgga gagacacgaa
     1381 tttcaggggc gtttgttttt cagtcgctta cactacaccc aacggcaaat cctgtcgatc
     1441 cttcccgaaa acccgatcca tttaccataa ctcccgttgt acgtacccct taaaacgccc
     1501 tcaatcgctt tctggcgcaa aaaacgcaaa aagacaagaa aacaccagga caaaccagaa
     1561 aaacgcgtca gaatcgtttt cagggcgttg cataactatg cacgaaagcg atgtgcgatc
     1621 ggtttcgtgc gttctgaaca ccgaacggct caaaacgaaa agttttctcc ctccggatgg
     1681 aaggttctgc acggagattt tagatcccat ctcccacatg gaatttttgt ggcacttcaa
     1741 caaaaaaagc gcgtcagaat cagcctgaca cgctttgtcc ttgtcaaatg aggattgcag
     1801 gttactgcgt ttccactccg gtcagttcag cagaggatga cggtgataca gtcccgccct
     1861 gaacatcacg gatatagttt gcctgacatg cagcaagaat aatggctgca agaagcaaca
     1921 aaataatccc tgttattttc ttcatacgat tccctgtata tatggattca ggaattcatc
     1981 cttgaacacc tgaatcaaac actttattac tcgtccctga tagtttaatt accagtcacc
     2041 atcacgataa tcgaaagaaa caaagtaatc gaacttacta atatcaataa catttttaaa
     2101 gtatggctgt aatattggca cccatctctt ttcaacatca aaccaaccgt tattaatatt
     2161 atcctttaaa ggcattccaa gacgttcaat aaccgaacca tcatcaccga aatcctttga
     2221 atactcacca cctttaaatt cctcattctt cttatcaaac caatgaatgt gtaatttaag
     2281 tcccataaca tcaccttaaa gatacttttt aatacttcgt ttcggatctg gacctttcaa
     2341 ctgcttaccg gttttagggt caaatgaacc aagatgctga ccatcactgg ctcgatagcc
     2401 ctcaagctca ccatgctgag agtcccactc ataaatttta cgccccttat ctccatacca
     2461 tctggcacgc ttacctccac caccttgctt tggagttttt ggcctcccct cttttaattc
     2521 ccccaaacct ttaatatcct cagttttagg agctggatga taatcatggc cataatcttt
     2581 agtgcctttt cggcgtttat ttttctcttc gtttaattta ttttcagcac tccttttctt
     2641 atcttctttc ttcttcctgc tttccatcgc agaactcaat gcagcatctg ctgctgactt
     2701 ctcttttgcg gcagcatcaa atgcagcctt cttattattt acatccgtct gtgcccgctg
     2761 ggccttaagc cctgccattt gccacattct gtgaccagca gccattggtt ctcgggcaaa
     2821 tcgctcgaat tgttttattt cagcctttgc atcagcaaga gttttattcg ctgcatcaag
     2881 ttcactttta cgagaattat aaacctgaac agctttagcc tgtcgttcct ggtttctggc
     2941 aacatcttta ttagcctgat tcagttctgc gcgggcctgt tcataattgc gttcagccac
     3001 ttccaccgga tgtgcgtcat tccattcctg ctggaggcgt ttttcctcat cctgacgttg
     3061 ttttacctgt gctggggtaa gaacatctgt cactgataca taaaccggct tctgcccact
     3121 ttctttcgga aagcgaatca ccgcttcatg tgaaccacca ccaaaggtaa atcccgcagg
     3181 aacggcagtc cggcctttat cctccgtaat accacgggga agggttgttg acaccggaag
     3241 gcctttgaca gtgctgactg tcagagaagg gacaccagga aatgatgcgt gaaatacacc
     3301 tggagtacgt gttggtttgg cattcacaac aggcacactc ataggcacac ctgcaactac
     3361 ggcaatatgc tgtcgtgtgt ccttcacaac atctgtcaca cgtttcgtca cgctgacagt
     3421 tgcctggtcc agcggaagtg tactgacctg aacattcgtc actgtttctg ccggtaatga
     3481 cgtcacaatc tttgacatca tattcgggtc atcttttgct atttcagatg gcagaatgcc
     3541 gtaaagcgca ataccccatg cgctgaattt aaacgggcct ttcagggctg caaagatatc
     3601 cgctattgcc gcagataaag cctcaccaga gacggcgata cccagcgttc cggcaccagg
     3661 agttgccaaa gcaggaaaac caaatgccac tgctgctacg gatgaattgc tgccaccacc
     3721 ggaattacca ttccccccgc cattaccatt gccggagcca cctccccagt gaacaccact
     3781 accggaaccg ccaccccatg ggttattttc cgaactccat cctgaaccgt cagaagcccc
     3841 accatttcca ccaagccccg tagggccgcc attaatgtta ccacctgtgt tatgtgcgcc
     3901 actgttgtgg tcgcgtccat ctccaccgct cataaaaatt ccctctttta agcgttaaaa
     3961 caattgatta tatagttgat actgtacata aaaccactgg ttttatgtac agaccaaaaa
     4021 gtactcgcca ttattttttc tgtcaagtca gggggggata ttttttggca gttcggagga
     4081 atcactcatg aaaacggtga acggaacgcg aaatcctttc gcgggtcgtg aggcgttgga
     4141 caagcgaagc gcgtcagttt ttccggctct tacccgtgac aggtttctcc accagtttat
     4201 cttccagtgt cacaactttc tgatacaccg ccgctgtggc gttgtagttc ctgtcgagaa
     4261 ttgttttcat cccccatccg agcacactga acataaccag aaaggccact gacaccccac
     4321 ccagccattt ccagctatac cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa
     4381 ttgtcgcaat atccctgttc agggcatccg cgtttgcctt catgtttgcg actgactctt
     4441 ccagccgctg aaaatcctga ctgatgcgct cacctatcag ccctgcaaca tccagcctct
     4501 gcacttcgtt tctgacttca tccctgacct gttttgccac accgtcagaa atatcccgat
     4561 atccgcgaag tgtttccctt gccacagcca gagaatcagc aacagctttc tgttgctcca
     4621 ttgcggtatg ttgcagatgc tccaccgata acaggagcgt gagtaatttt tcttctgtgc
     4681 tggccatgtt cagaatccca tagaaaatcc acgtgacggt gatttaaccc gttcaggttc
     4741 agttcggttc tgtctgctca ctctgtctct catgtcaaag ccatgctccc gcgcaacatc
     4801 atgttgcagc cttctgacca gttcatgttg cttaaccgga gtgtccagcc attccctgat
     4861 gtcatgcccg accgttcgcg cctgttcaag atggcgggaa tagtccggtt cagaccgttt
     4921 ttgcatgatg tcatgacgct gggcatcatc aagccgtccg gcagcttcaa aacccagatg
     4981 cacttcggga cgtcgttcaa tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct
     5041 ttcaaagcca tgctttctca tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc
     5101 ccgcaactgc tctttttgct gagtgagagt caggcttccg gaatcctttt tcgcacctcc
     5161 ccgctcggga tacctggcgt tatagcgccg gaaatactgt tccggtgtac gttcaatgcc
     5221 atcattcaca cgctggctca tcatgatatg cgcatgaggc tgttcgccgc cgtcaatgct
     5281 cgctttcgga ttgtgaatgg caaaactcca ggcgtgccgt tcgccagctt cctgacgtac
     5341 aaaatcctgc accagttcaa ggcgctgttc cgctgtcagt tcacggggta atgcaatttc
     5401 aagttcccga taggtcgaac cgttcgcacg ttcaaactgg tcagccgcct gccagaaatg
     5461 tgagggatta tctcgcgccc attccggcat gtttccgtgt gcggaatatt ccaaatcctg
     5521 acggtcgcgg tatttctctt tgcgctcgat gtaatcggcg tggtttgcag cctgcccctt
     5581 gccaccaaat ttaacgctga ggtgataggt tgccacggtt tcagccctcc gcaggagcgc
     5641 actgtttctc gacgaaggag agaaacatat aagtgcgctt ccttctaatt ttacgaatta
     5701 gcacggaaga taccccctgc acccaccgac cgcaagcgat atactgcacg accacaacac
     5761 acagcaaaaa acaccaggga gcaccacgaa catgcgcatc agcaacatcg aatggctgaa
     5821 gaaacggatc ggattcatca ggaagctcgg agaacagacc gcacgacaga gacagattat
     5881 tgacctgctc gacaacgaag caggactcac agaacaggaa cgcaaactcc tccacgtact
     5941 ggcaacagcg gaaaaaaacg acttacaggc acaggagagc gaacgaaaac aggcggtcca
     6001 aaaacgaatc gaggg
//
LOCUS       DAZSBF010000237         6012 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZSBF010000237 DAZSBF010000000
VERSION     DAZSBF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.242"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1584
                     /gene="tssI"
                     /locus_tag="ACHXWD_003688"
     CDS             <1..1584
                     /gene="tssI"
                     /locus_tag="ACHXWD_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR7622485.1"
                     /translation="STDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSAQ
                     IRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQM
                     DGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGRR
                     GSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFN
                     WDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTYH
                     QENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNNR
                     TTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRAL
                     TVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDT
                     GQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAAS
                     KSPPKTPDEKQDTPDMREY"
     gene            1652..5647
                     /locus_tag="ACHXWD_003689"
                     /pseudo
     CDS             1652..5647
                     /locus_tag="ACHXWD_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1434 a         1534 c         1941 g         1103 t
ORIGIN      
        1 agtaccgacc agagcctggt cctgtgcgac accgtgcgtt atctgccgga gtcctttgag
       61 atcccctgga acccgaacac ccgtaccgag gtaagtaccc tctgcatcag ccagtttcgc
      121 tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag actacacctt taaacgtccc
      181 ggctggccag ggcgttttga tcaggaaggc cagtaccagg attaccagcg cacgcagtat
      241 gaagtgtatg actaccccgg acgtttcaag ggtgcccacg ggcagaactt tgcccgctgg
      301 cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg gaacaagccg ttcgccggag
      361 atatggccgg gacggcgaat tgtgctgacg gggcatccgc aggcgaacct gaaccgggaa
      421 tggcaggtgg tggcgagcga tcttcacggc gaacagccgc aggcggtacc gggacgcagg
      481 ggttcaggca ccacgctgga gaaccatttt gcggtaatcc cggcagacag aacatggcgg
      541 ccacagccgt tgatgaaacc gctggtggat ggcccgcaga gcgccgtcgt gacgggaccg
      601 gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc gggtgaaatt taactgggac
      661 cgctataacc cgtcaaacca ggacagttca tgctggatcc gtgtggcaca ggcgtgggca
      721 ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc aggaggtgat tgtggacttc
      781 ctcaacggcg acccggacca gccgattatt atggggcgta cctaccacca ggaaaaccgc
      841 acacccggca gcctgccggg gacgaagacg cagatgacca ttcgttcgaa aacctataag
      901 ggcagcgggt ttaatgaact gaagtttgac gatgcgacag ggaaagaaca ggtctacatc
      961 cacgcgcaga agaacatgaa caccgaggtg ctgaataacc gcaccacgga tgtgataaac
     1021 aaccatgctg aaaccattgg caacaatcag atgattgcgg ttaccaacaa tcagatagag
     1081 acggtgggcg ttaaccagat agagacggtg ggcagtaacc agataattaa agtgggttct
     1141 gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg tgggcgtggc gtatcagacg
     1201 acggtaggtg gcattatgaa cacctcggtg gcactgatgc agtcctcgca gatcggtttg
     1261 cataaatcgt tgagggtcgg gctgggttat gacgtcaaag tcggaaataa cgttaccttc
     1321 accgttggta aaacgaaaaa ggatgatacc gggcagaccg cgatttactc cgccggtgag
     1381 catctggagc tctgctgtgg taaggcaagg ctggtgctga cgaaggacgg acaaattttt
     1441 ctcaacggca caaaaattca tttgcagggt aaggagcagg ttaatggtga ctcactgttg
     1501 attaactgga actgtgcagc ctcgaaatcc ccaccgaaga cccctgatga aaagcaggat
     1561 acgccggata tgagagagta ctgatcccgt acctgttcgg tattcagtaa caaaccagca
     1621 ttaaaaataa acagaagaag gaatttgaat catgagcgga aaaccagcgg cgcgtcaggg
     1681 agatatgact cagtatggcg gtcccattgt ccagggttcg gcaggtgtaa gaattggcgc
     1741 gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg acttcgggca acccggtaaa
     1801 tccgctgctg ggggcgaagg tgctgcccgg cgagacggac cttgcgctgc ccggcccgct
     1861 gccgttcatt ctctcccgca cctacagcag ctaccggacg aagacgcctg caccggtggg
     1921 cgttttcggc cccggctgga aagcgccttc tgatatccgc ttacagctac gtgatgacgg
     1981 actgatactc aacgacaacg gcgggcggag cattcacttt gagccgctgc tgccggggga
     2041 ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc ggtggtaagg cagcacagcc
     2101 ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc cggatatccg gttaagcccg
     2161 catctttacc tggcgaccaa cagcgcacag gggccgtggt ggatactggg gggggctgag
     2221 cgggtgccgg gtgctgagga cgtactgcca gcgccgctgc cgccgtaccg ggtgcttacc
     2281 gggatggcgg acgctgacgt accggcgtga ggccgccggt gacctggccg gggaaatcac
     2341 cgacgtgacg gacggtgccg ggcgggagtt ccgtctggtg ctgaccacgc aggcgcagcg
     2401 tgcggaagag gcccgcacct cttcgctatc ttcttctgac agttcccgcc ctctctcagc
     2461 ctcagcgttc cccgacacac tgcccggtac cgaatacggc cccgacaggg gtatccgcct
     2521 ttcggcggtg tggttgatgc acgacccggc atacccggag agcctgcccg ctgcgccact
     2581 ggtgcggtac acgtatacgg aagccggtga actgctggcg gtatatgacc gcagcaatac
     2641 gcaggtgcgc gctttcacgt atgacgcgca gcgcccgggc cggatggtgg cgcaccgtta
     2701 cgcgggaagg ccggagatgc gctaccgcta cgacgatacg gggcgggtgg tggagcaact
     2761 gaacccggca gggttaagct accgctatct ttatgagcag gaccgcatca ccgtcaccga
     2821 cagcctgaac cggcgtgagg tgctgcatac agaaggcggg gccgggctga aacgggtggt
     2881 gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg tatgacgcgg caggaaggct
     2941 cacggcgcag acggacgcgg cgggacggcg gacagagtac ggtctgaatg tggtgtccgg
     3001 cgatatcacg gacatcacca caccggacgg gcgggagacg aaattttact ataacgacgg
     3061 gaaccagctg acggcggtgg tgtacccgga cgggctggag agccgccggg aatatgatga
     3121 accgggcagg ctggtatcgg agacatcgcg cagcggggag acagtacgct accgctacga
     3181 tgacgcgcac agtgagttac cggcgacgac aacggatgcg acgggcagca cccggcagat
     3241 gacgtggagc cgctacgggc agttgctggc gttcactgac tgctcgggct accagacccg
     3301 ctatgaatac gaccgcttcg gccagatgac ggcggttcac cgcgaggaag gtatcagcct
     3361 ttaccgccac tatgacaacc gtggccggtt aacctcggtg aaagacgcac agggccgtga
     3421 aacgcagtat gagtacaacg ccgcaggcga cctgactgcc gttatcaccc cggacggcaa
     3481 ccggagcgag acacagtacg atgcgtgggg aaaggcggtc agcaccacgc agggcgggct
     3541 gacgcgcagt atggagtacg atgctgccgg acgtgtcatc agtctgacta acgagaacgg
     3601 cagtcacagc gacttcagct acgatgcgct ggaccggctg gtacagcagg gcggctttga
     3661 cgggcggacg caacgttatc attatgacct gaccggaaaa ctcacgcaga gtgaggatga
     3721 ggggcttgtc accctctggc actacgatgc gtcggaccgc atcacgcacc ggacggtgaa
     3781 cggcgacccg gcagagcagt ggcagtatga cggccacggc tggctgagag aaatcagcca
     3841 cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac gacgataagg gccgcctgac
     3901 gggcgaacgg cagacggtgg agaacccgga gacgggggaa ctgctgtggc agcatgagac
     3961 gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc acgccggaca gcctgccgcc
     4021 ggtggagtgg ctgacctacg gcagcggtta cctggcgggc atgaagctgg gcgggacgcc
     4081 gctggtcgag tatacgcggg acaggctgca ccgtgagacg gtgcgcaccc tcgcaccgga
     4141 cctggacatc cgcatcccgt atgccaccga cccggcggga aaccgcctgc cggacccgga
     4201 gctacacccg gacagcacgc tcacggcgtg gccggataac cgcatcgcag aggatgcgca
     4261 ctatgtttac cactatgatg aatacggcag actgacggag aagacggacc gcatcccgac
     4321 gggtgtgata cggacggacg acgagcggac ccaccactac gacagccagc accgcctggt
     4381 gttccacacg cggatacagc atggcgagcc actggtcgag agccgctacc tctacgaccc
     4441 gctgggacgg cgaatggcga aacgggtatg gcggcgggag cgtgacctga cggggtggat
     4501 gtcgctgtcg cgtaaaccgg aggagacatg gtacggctgg gacggcgaca ggctgacgac
     4561 ggtacagacc gacaccacac gtatccagac ggtatacgag ccgggaagct tcacgccgct
     4621 catccgggtc gagacagaga acggcgagcg ggagaaagcg cagcgccgca gcctggcgga
     4681 gaagctgcag caggaaggga gcgaggacgg gcacggcgtg gtatttccgg ctgaactggt
     4741 gcggctgctg gacagactgg aggaagaaat ccgggcagac cgcgtgagca gtgaaagccg
     4801 ggtgtggctt gcgcagtgcg ggctgacggt ggagcaactg gccagacagg tggagccgga
     4861 atacacacca gtgcgaaacg ttcatcttta ccactgcgac catcggggcc agccgctggc
     4921 gctcatcagc gaagacggcg atacggtgtg gagcggggcg tatgatgagt ggggcaacca
     4981 gctaaatgag gagaacccgc atcatgtgta tcagccatac cgtctgccgg ggcagcagta
     5041 tgatgaggag tcggggctgt actacaatcg tcaccggtac tatgatccgt tgcagagacg
     5101 gtacatcacc caggacccca ttgggctgaa aggtgggtgg aatctgtatc agtatccgct
     5161 gaatccggtg agcaggatag atccgttggg gttattctca tgtgagagtt gtagtcattt
     5221 tatagatgat ataaataata aactggataa ccttccgttt aacccgacaa aaatgtttac
     5281 agctagttta aatactgcta attcagcaag gctgtatgca agtggcatga caaaattcgg
     5341 tataagtgtt gctgccgatg gaacgatcat tgctgcccca gtcggtgcag cttctatggc
     5401 tttaggggcg tggaatataa aatctgcgac agctgcatat aacagagcaa atttacaatt
     5461 atcagaatca cttcaggaaa atggtagaga taggtgttgg agaaatttat tgggtatttt
     5521 accttatggt gagcagtttg atgaccctgg tgaaccatca atattagacg tttatggtga
     5581 aaagattgag aatatcaaag attctccaat gaagtttttt gaagagcttg ggacgctggg
     5641 gttgtaatgt gtatttttaa tgatattttt atgtttgtgt ttattgttag tagtatactt
     5701 gtaaccgtgc attttgtgta ttctttattt tttaaaagtg gaaaggtaaa agataaggta
     5761 aaaaaatgga ttaaagtaat gtgtgattta ttttggggga taggatagtg gtgtgatatg
     5821 gataaatata aattacagtg gctggcatat ttcgacctga cgccggagta gataaaaaaa
     5881 cagacaggat tggagtatag caagcgtctg acaatctctc gggacagtag tatgatgaag
     5941 aggcggggtt gtactataac cgtaaccggt actacgatcc gctaaagggc aagtatatga
     6001 ctcagggaaa ga
//
LOCUS       DAZSBF010000238         5961 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZSBF010000238 DAZSBF010000000
VERSION     DAZSBF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.243"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHXWD_003690"
     tRNA            292..368
                     /locus_tag="ACHXWD_003690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHXWD_003691"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHXWD_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR7622486.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHXWD_003692"
     CDS             complement(1357..2247)
                     /locus_tag="ACHXWD_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR7622487.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHXWD_003693"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHXWD_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR7622488.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHXWD_003694"
                     /pseudo
     CDS             complement(5207..5938)
                     /gene="nfrB"
                     /locus_tag="ACHXWD_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
BASE COUNT         1354 a         1564 c         1556 g         1487 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg agttgttcgg
     5941 taatgactcc aacttattga t
//
LOCUS       DAZSBF010000239         5851 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZSBF010000239 DAZSBF010000000
VERSION     DAZSBF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.244"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHXWD_003695"
     CDS             complement(38..400)
                     /locus_tag="ACHXWD_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR7622489.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHXWD_003696"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHXWD_003696"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR7622490.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHXWD_003697"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHXWD_003697"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR7622491.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHXWD_003698"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHXWD_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR7622492.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHXWD_003699"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHXWD_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR7622493.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHXWD_003700"
     CDS             5321..5683
                     /locus_tag="ACHXWD_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR7622494.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1474 a         1448 c         1520 g         1409 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgagat
     5821 tctgaaaatg catttttacc gaaatagcca c
//
LOCUS       DAZSBF010000240         5791 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZSBF010000240 DAZSBF010000000
VERSION     DAZSBF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.245"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            16..1005
                     /gene="ldhA"
                     /locus_tag="ACHXWD_003701"
     CDS             16..1005
                     /gene="ldhA"
                     /locus_tag="ACHXWD_003701"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR7622495.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHXWD_003702"
     CDS             1116..1538
                     /gene="hslJ"
                     /locus_tag="ACHXWD_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR7622496.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1535..1801)
                     /locus_tag="ACHXWD_003703"
     CDS             complement(1535..1801)
                     /locus_tag="ACHXWD_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR7622497.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHXWD_003704"
     CDS             2075..5599
                     /gene="nifJ"
                     /locus_tag="ACHXWD_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR7622498.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1463 a         1487 c         1483 g         1358 t
ORIGIN      
        1 actggagaaa gtcttatgaa actcgccgtt tatagcacaa aacagtacga caagaagtac
       61 ctgcaacagg tgaacgagtc ctttggcttt gagctggaat tttttgactt tctgctgacg
      121 gaaaaaaccg ctaaaactgc caatggctgc gaagcggtat gtattttcgt aaacgatgac
      181 ggcagccgcc cggtgctgga agagctgaaa aagcacggcg ttaaatatat cgccctgcgc
      241 tgtgccggtt tcaataacgt cgaccttgac gcggcaaaag aactggggct gaaagtagtc
      301 cgtgttccag cctatgatcc agaggccgtt gctgaacacg ccatcggtat gatgatgacg
      361 ctgaaccgcc gtattcaccg cgcgtatcag cgtacccgtg acgctaactt ctctctggaa
      421 ggtctgaccg gctttactat gtatggcaaa acggcaggcg ttatcggtac cggtaaaatc
      481 ggtgtggcga tgctgcgcat tctgaaaggt tttggtatgc gtctgctggc gttcgatccg
      541 tatccaagtg cagcggcgct ggaactcggt gtggagtatg tcgatctgcc aaccctgttc
      601 tctgaatcag acgttatctc tctgcactgc ccgctgacac cggaaaacta ccatctgttg
      661 aacgaagccg ccttcgatca gatgaaaaat ggcgtgatga tcgtcaatac cagtcgcggt
      721 gcattgattg attctcaggc agcaattgaa gcgctgaaaa atcagaaaat tgggtcgttg
      781 ggtatggacg tctatgagaa cgaacgcgat ctattctttg aagataaatc caacgacgtg
      841 atccaggatg acgtattccg tcgcctgtct gcctgccaca acgtgctgtt taccgggcac
      901 caggcattcc tgacagcaga agctctgacc agtatttctc agactacgct gcaaaactta
      961 agcaatctgg aaaaaggcga aacctgcccg aacgaactgg tttaatcttg ccgctcccct
     1021 gcattccagg ggagcttatt cagataatcc ccaaagacct ttcatcttct attcttaaaa
     1081 tagccctgag tcagaaactg taattgagaa ccacaatgaa gaaagtagcc gcgtttgttg
     1141 cgctaagcct gctgatggcg ggatgtgtaa gtaatgacaa aattgctgtt acgccagaac
     1201 agctacagca tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata
     1261 aaaatccgcc agaaatcagc tttggtgaaa aaatgatgat ttccggcagc atgtgtaacc
     1321 gctttagcgg tgaaggcaaa ctgtctaatg gtgaactgac agccaaaggg ctggcaatga
     1381 cccgtatgat gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc
     1441 tgaaagaagg tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac
     1501 agacattaac ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg
     1561 caagtgactg ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac
     1621 catccagttg acgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac
     1681 tactggacat cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag
     1741 gttcactact acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca
     1801 tctcttcttc ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga
     1861 gggcggcgtc gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt
     1921 ttttgccctc tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa
     1981 atgcagaaga aagccatttg ctaaaattga aggattacta ctgggcgcgc agcaatttcg
     2041 tgcgcccctc atttgcgcaa tgtaagggtg tcatatgatt actattgatg gtaatggcgc
     2101 ggttgcttcg gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag
     2161 ttccacgatg gcagaacagg ctgatgcctg ggccggaaac ggcttgaaga acgtttgggg
     2221 agacacacca tgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg
     2281 cgctttgcag acgggtgccc tttcaacatc gtttacgtca tcgcagggtt tgctgctgat
     2341 gatcccgaca ctgtacaaac tggcaggcga actcacaccg tttgtcctgc atgtagcggc
     2401 acgtaccgtt gccacacatg cactctctat ttttggcgat cattccgacg tcatggcggt
     2461 gcgccagacg ggttgcgcga tgttgtgtgc ggcaaacgtc caggaagcgc aagactttgc
     2521 tctcatttcg cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg
     2581 tttccgcacg tcccacgaaa tcaacaaaat tgtcccgctg gccgatgaca cgattcttga
     2641 tctcatgccg caggccgaaa ttgatgctca tcgcgcccgg gcgctcaacc cggaacatcc
     2701 ggtgatccgt ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa
     2761 cccctggtac aacgcagtct atgaccatgt tgaacaggcg atgaatgatt tctctgccgc
     2821 gacaggtcgt cagtatcagc cgtttgaata ttacgggcat ccgcaagctg aacgggtgat
     2881 tatcctgatg ggctcagcca ttggcacctg tgaagaagtg gttgatgaat tgctaacccg
     2941 tggcgaaaaa gtcggcgtgc tgaaagttcg cctgtaccgc cccttctcag ccaaacactt
     3001 gctacaaact ctaccgggat ccgtacgcag cgtggcggta ctggacagaa ccaaagaacc
     3061 cggtgcccag gcagaaccgc tctatctgga tgtgatgacc gcactggcag aagcctttaa
     3121 taatggcaag cgcgaaactc tgccccgtgt cattggtggg cgctatggtc tttcatccaa
     3181 agaatttggc ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa
     3241 agcgcgcttt acggttggta tttacgatga tgtaaccaat ctgtccctgc cgttgccgga
     3301 aaacacccag ccaaactcgg cgaaacttga agccttgttt tatggccttg gtagtgatgg
     3361 cagcgtttcc gcgaccaaaa acaatatcaa gattatcggt aattccacgc cgtggtacgc
     3421 acagggctat tttgtttacg actccaaaaa agcgggcggc ctgacggttt ctcaccttcg
     3481 cgtgagcgaa cagccgattc gttccgctta tctcatttcc caggctgatt ttgttggctg
     3541 ccaccagttg cagtttatcg ataaatatca gatggctgag cgtttaaaac ctggcggcat
     3601 tttcctgctc aacacgccgt acagcgcaga tgaagtgtgg tcgcgcttgc cgcaagaagt
     3661 tcaggccgtg ttaaaccaga aaaaagcgcg cttctatgtg attaacgcgg cgaaaatcgc
     3721 ccgcaaatgt ggcctggcgg cccgtattaa taccgtcatg cagatggctt ttttccatct
     3781 gacgcaaatt ctgcctggcg atagcgccct cgcagaattg cagggtgcga ttgccaaaag
     3841 ttacagtagc aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcgcg
     3901 tgaatccgta gaagaagttc cgttgcaacc ggtaaatccg cacagcgcca atcgaccgcc
     3961 agtggtttcc gatgccgccc ctgatttcgt gaaaaccgta accgcagcga tgctcgcagg
     4021 gcttggtgat gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac
     4081 cactcgctgg gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg
     4141 tacccaatgt aaccactgcg tcgcagcttg cccacactca gctattcgcg caaaagtggt
     4201 accacccgaa gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg
     4261 tgatatgcgc gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa
     4321 cctgtgcgtc gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa
     4381 tatgatgtcg cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa
     4441 cctgccagaa atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat
     4501 tacaccgctg tttgaatatt ccggtgcttg ctccggttgt ggcgagacgc cgtatattaa
     4561 attgctgact cagctctatg gcgaccggat gttgatcgct aacgccaccg gctgttcttc
     4621 aatttatggc ggtaacctgc cctctacacc gtataccacc gatgccaacg gtcgtgggcc
     4681 ggcatgggcg aactctctat ttgaagataa tgccgaattt ggccttggtt tccgcctgac
     4741 ggtcgatcaa caccgtgtcc gcgtgctgcg tctgctggat caatttgccg ataaaatccc
     4801 ggcggaatta ctgacggcgt tgaaatcaga cgctacgcca gaggttcgtc gagaacaggt
     4861 cgcagcttta cgccagcaac tcaacgatgt tgccgaagca catgaactgc tacgtgatgc
     4921 agatgcactg gtggaaaaat caatctggct gattggtggt gatggctggg cttacgatat
     4981 cggctttggc ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct
     5041 ggatacgcaa tgctattcca acaccggtgg tcaggcgtcg aaagcgacac cgctgggtgc
     5101 agtaactaaa tttggcgagc acggcaaacg taaagcgcgt aaagatcttg gcgtcagtat
     5161 gatgatgtac ggtcatgttt atgtggcgca gatttctctc ggcgcgcagc tgaaccagac
     5221 ggtgaaagcg attcaggaag cggaagcgta tccgggacca tcgctgatca ttgcttatag
     5281 cccgtgtgaa gagcatggtt acgatctggc actcagccat gaccagatgc gccaactcac
     5341 agctaccggc ttctggccgc tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact
     5401 gccgctggcg ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga
     5461 gcaacgtttc cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga
     5521 tgctgcagct gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga
     5581 aaaaagcaac accgattaaa tgctctggat aaggattacc caattctaaa aaaaagcccg
     5641 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
     5701 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa
     5761 attaaaagta taatttttat tttgtatgta t
//
LOCUS       DAZSBF010000241         5730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZSBF010000241 DAZSBF010000000
VERSION     DAZSBF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.246"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(120..596)
                     /locus_tag="ACHXWD_003705"
     CDS             complement(120..596)
                     /locus_tag="ACHXWD_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR7622499.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(655..1944)
                     /gene="bioA"
                     /locus_tag="ACHXWD_003706"
     CDS             complement(655..1944)
                     /gene="bioA"
                     /locus_tag="ACHXWD_003706"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR7622500.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2031..3071
                     /gene="bioB"
                     /locus_tag="ACHXWD_003707"
     CDS             2031..3071
                     /gene="bioB"
                     /locus_tag="ACHXWD_003707"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR7622501.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3068..4222
                     /gene="bioF"
                     /locus_tag="ACHXWD_003708"
     CDS             3068..4222
                     /gene="bioF"
                     /locus_tag="ACHXWD_003708"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR7622502.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4209..4964
                     /gene="bioC"
                     /locus_tag="ACHXWD_003709"
     CDS             4209..4964
                     /gene="bioC"
                     /locus_tag="ACHXWD_003709"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR7622503.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            4957..5634
                     /gene="bioD"
                     /locus_tag="ACHXWD_003710"
     CDS             4957..5634
                     /gene="bioD"
                     /locus_tag="ACHXWD_003710"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR7622504.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1347 a         1534 c         1608 g         1241 t
ORIGIN      
        1 actgtttctc gttcagcttt ttatactaac ttgagcgaaa cgggaaggta aaaagacaaa
       61 aagttgtttt taataccttt aagtgatacc agatggcatt gcgccatctg gcagagtgat
      121 taactaaaca tcgcagtaat cgaggcgctt gccagagagt ggaaatgaac gttaaacccg
      181 accatcgcgc cgctggcacc ttcatcgaca tcaatacgtt ctatatccag cgcgtgaacg
      241 gtaaaaatgt agcgatgagt ttcgcctttc ggcggtgctg cgccatcgta cccggtttta
      301 ccaaagtcgg tacgcgtctg caaaacgccg tctggcattg ctaccagacc agagccaaac
      361 ccttgcggta atacgcgggt atcagcgggt aagttaacaa ctacccagtg ccaccagccg
      421 gagccggttg gcgcatccgg gtcgtagcag gtgacaacaa aacttttcgt tcccgcagga
      481 acatcatccc acgccagatg cggtgaaata ttatcgccat cgtaacccat gccgttaaag
      541 acatgacgat gcggcaattt atcgccatcg cgcagatcgt tactgatgag tttcatgaac
      601 cctcctttct tgtttgcaga aagtgtagcc agaaaccctc acgcggactt ctcgttattg
      661 gcaaaaaaat gtttcatcct gtaccgcgcg gttaaccgct gcggtcagac gctgcaactg
      721 ttgcgggaga ataatatagg gcggcatcag gtaaatcagt ttgccaaaag gccggatcca
      781 gacaccctgt tcgacaaaga atttttgcag cgccgccata ttcaccggat gagtggtttc
      841 gaccacgcca atggccccca gtacgcgcac atcggcaacc atttcggcat cacgggcggg
      901 ggcaagttgc tcgcgcagct gtacttcaat atccgccacc tgttgctgcc agtcgccaga
      961 ttcgagaatc gccaggctgg cgtttgctgc cgcgcaggcc agcggattgc ccataaaagt
     1021 tggcccatgc ataaagcaac cggcttcacc gttactgatg gtttctgcaa cctcgcgcgt
     1081 ggtgagtgtg gcggaaaggg tcattgtgcc gccggttaag gctttaccga ggcacaaaat
     1141 gtccggcgcg atttctgcat gttcacaggc aaacagtttc ccggtacgac caaatccagt
     1201 ggcgatctcg tcggcaatca gcaagatacc ttcgcgatcg catattttgc ggattcgttt
     1261 taaccattcc ggatggtaca tgcgcatccc gcctgcgccc tggacaatcg gctcaatgat
     1321 caccgccgcg atttcatgac gatgcgccgc catcaggcgg gcaaagccca ccatatcgcg
     1381 ctcatcccat tcgccatcca tacggctttg tggggcggga gcaaacaagt tttctggcag
     1441 atagcctttc cacaggctgt gcattgagtt gtccggatcg cacaccgaca tcgcgccaaa
     1501 ggtatcgcca tgataaccat tgcggaaggt cagaaaacgc tggcgcgctt cgcctttggc
     1561 ttgccagtac tgcaacgcca ttttcatcgc cacttccacc gctacggaac cggagtccgc
     1621 gagaaaaacg cactccagcg gttgcggcgt catcgccacc agtttgcggc acagctcaat
     1681 ggctggcgca tgggtgatac cgccaaacat cacatgcgac atggcatcaa tttgcgactt
     1741 catcgccgca ttaagctgcg ggtgattgta gccgtggatc gccgcccacc aggacgacat
     1801 accgtcaacc aggcgtctgc cgtcagacaa aatcagctcg caaccttcgg cgctcaccac
     1861 cggataaacc ggcagcgggg aggtcatgga tgtgtatggg tgccagatat ggcgttggtc
     1921 aaaggcaaga tcgtccgttg tcataatcga cttgtaaacc aaattgaaaa gatttaggtt
     1981 tacaagtcta caccgaatta acaacaaaaa acacgttttg gagaagcccc atggctcacc
     2041 gcccacgctg gaccttgtcg caagtcacag aattatttga aaaaccgttg ctggatctgc
     2101 tgtttgaagc gcagcaggtc catcgccagc atttcgatcc tcgtcaggtc caggtcagca
     2161 cgttgctgtc gattaagacc ggagcttgtc cggaagattg caaatactgc ccgcaaagct
     2221 cacgctacaa aaccgggctg gaagccgagc ggttgatgga agttgaacag gtgctggagt
     2281 cggcgcgcaa agcgaaagcg gcaggatcga cgcgcttctg tatgggcgcg gcgtggaaga
     2341 atccccacga acgcgatatg ccgtacctgg aacaaatggt gcagggggta aaagcgatgg
     2401 ggctggaggc gtgtatgacg ctgggcacgt tgagtgaatc tcaggcgcag cgcctcgcga
     2461 acgccgggct ggattattac aaccacaacc tggacacctc gccggagttt tacggcaata
     2521 tcatcaccac ccgcacttat caggaacgcc tcgatacgct ggaaaaagtg cgcgatgccg
     2581 ggatcaaagt ctgttctggc ggcattgttg gcttaggcga aacggtaaaa gatcgcgccg
     2641 gattattgct gcaactggca aacctgccga cgccgccgga aagcgtacca atcaacatgc
     2701 tggtgaaggt gaaaggcacg ccactggcgg ataacgatga tgtcgatgcc tttgatttta
     2761 ttcgcactat tgcggtagcg cggatcatga tgccgacctc ttacgtgcgc ctttctgccg
     2821 gacgcgagca gatgaatgaa cagactcagg cgatgtgctt tatggctggc gcaaactcga
     2881 ttttctacgg ttgcaaactg ctgaccacgc cgaatccgga agaagataaa gacctgcaac
     2941 tgttccgcaa actggggcta aatccgcagc aaactgccgt gctggcaggg gataacgaac
     3001 aacagcaacg tcttgaacag gcgctgatga ccccggacac cgacgaatat tacaacgcgg
     3061 cagcattatg agctggcagg agaaaatcaa cgcggcgctc gatgcacggc gtactgccga
     3121 tgccctgcgt cgccgttatc cggtggcgca aggagccgga cgctggctgg tggcggatga
     3181 tcgccagtat ctgaactttt ccagtaacga ttatttaggt ttaagccatc atccgcaaat
     3241 tatccgcgcc tggcagcagg gcgcagaaaa gtttggtgta ggtagcggcg gttccgggca
     3301 tgtcagcggt tatagcgtgg tgcatcaggc gctggaagaa gaactggccg agtggcttgg
     3361 ctattcgcgg gcgctgctgt ttatctctgg ttttgccgcg aatcaggcag tcattgccgc
     3421 gatgatggcg aaagaggacc gtattgttgc cgaccggctt agccatgcct ccttgctgga
     3481 ggctgccagt ttaagcccgt cgcagcttcg ccgttttact cataacgatg tcgctcatct
     3541 ggcgcgactg cttgcttccc cctgtccggg gcagcaactg gtggtgacag aaggcgtgtt
     3601 cagcatggac ggcgatagcg cgccactggc ggaaatccag caggtaacgc aacagcacaa
     3661 tggctggttg atggtcgatg atgcccacgg cacgggcgtt atcggggagc aggggcgcgg
     3721 cagctgctgg ctgcaaaagg taaaaccaga attgctggtg gtgacttttg gcaaaggatt
     3781 tggcgtcagt ggggctgcgg tgctttgctc cagtacggtg gcggattatc tgctgcaatt
     3841 cgcccgccac cttatctaca gcaccagtat gccgcccgct caggcgcagg cattacgtgc
     3901 gtcgctggcg gtcattcgcc gtgatgaggg ggatgcacgg cgcgaaaaac tggtgtcact
     3961 cattgcgcgt tttcgtgccg gagtacagga tctgccgttt acgcttgctg attcatgcag
     4021 cgccatccag ccattgatcg tcggtgataa cagccgtgcg ttacaactgg cagaaaaact
     4081 gcgccagcaa ggctgctggg tcacggcgat tcgcccgcca accgtacccg ctggtactgc
     4141 gcgactgcgc ttaacgctaa ccgcagcgca tgaaatgcag gatatcgacc gtctgctgga
     4201 ggtgctgcat ggcaacggtt aataaacaag ccattgcagc ggcatttggt cgggcagccg
     4261 cgcactatga gcaacacgca gagttacagc gccggagtgc tgacgtctta ctggcaatgc
     4321 tcccacagcg taaatacacc cacgtactgg acgcggggtg tgggccaggc tggatgagcc
     4381 gccactggcg agagcgtcac gcacaggtga cggcgttaga tctctcaccg ccaatgcttg
     4441 tgcaagcccg ccagaaagat gctgcagacc attatctggc gggagatatc gaatccctgc
     4501 cgttagcgac tgcgacgttc gatcttgcat ggagcaatct cgcggtgcag tggtgcggta
     4561 atttatccac ggcactccgc gagctgtatc gggtggtgcg ccccggaggc gtggtggcgt
     4621 ttaccacgct ggtgcaggga tcgttacccg aactgcatca ggcgtggcag gcggtggacg
     4681 agcgtccgca tgctaatcgc tttttaccgc cagatgaaat cgaacagtcg ctgaacggcg
     4741 tgcattatca acatcatatt cagcccatca cactatggtt tgatgatgcg ctcagtgcca
     4801 tgcgttcgct gaaaggcatc ggtgctacgc atcttcatga agggcgcgac ccgcgaatat
     4861 taacgcgttc gcaattgcag cgattgcaac tggcctggcc gcaacaacag gggcgatatc
     4921 ctctgacgta tcatcttttt ttgggagtga ttgctcgtga gtaaacgtta ttttgtcacc
     4981 ggaacggata ccgaagtggg gaaaaccgtc gccagttgtg cacttttaca agccgcgaag
     5041 gcagtaggtt atcggacggc aggttataaa ccggtcgcct ctggcagcga aaagaccccg
     5101 gaaggcttac gtaatagtga cgcgctggcg ttacagcaca acagcagcct gcagctggat
     5161 tacgcaacag taaatcctta caccttcgca gaacccactt cgccgcacat catcagcgcg
     5221 caagagggca ggccgataga atcattggta atgagcgccg gattacgcgc gcttgaacaa
     5281 caggctgact gggtgttagt ggaaggtgct ggcggctggt ttacgccgct ttctgacact
     5341 ttcacttttg cagattgggt aacacaggaa caactgccgg tgatactggt agttggtgtg
     5401 aaactcggct gtattaatca cgcgatgttg actgcgcagg caatacaaca cgccggactg
     5461 actctggcgg gttgggtggc gaacgatgtt acgcctccgg gaaaacgtca cgctgaatat
     5521 atgaccacgc tcacccgcat gattcccgcg ccgctgctgg gagagatccc ctggcttgca
     5581 gaaaatccag aaaatgcggc aaccggaaag tacataaacc ttgccttgtt gtagccattc
     5641 tgtatttggt taaattgcga gcgagatcgc gtcttcgatt gactgcaatt taaccaatta
     5701 aattctaaaa taatcacgaa aaaaatttta
//
LOCUS       DAZSBF010000242         5683 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZSBF010000242 DAZSBF010000000
VERSION     DAZSBF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.247"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(266..660)
                     /locus_tag="ACHXWD_003711"
                     /pseudo
     CDS             complement(266..660)
                     /locus_tag="ACHXWD_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            603..1109
                     /locus_tag="ACHXWD_003712"
     CDS             603..1109
                     /locus_tag="ACHXWD_003712"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR7622505.1"
                     /translation="MLEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHA
                     DSYGYRPGKSAHDALKQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWV
                     ILYCRRWMEAPMQSCENGELITRTRGTPQGGVISPLLANLFHHDAFDLWMEREYRGYR
                     LRGTLTIL"
     gene            1124..1651
                     /locus_tag="ACHXWD_003713"
     CDS             1124..1651
                     /locus_tag="ACHXWD_003713"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFR7622506.1"
                     /translation="MSDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSF
                     TFLGYDFKVRTLKNFKGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARR
                     YNAIVRGWIEYYGKFWSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEY
                     PKLFVHWYLLRASNE"
     gene            1734..1913
                     /locus_tag="ACHXWD_003714"
                     /pseudo
     CDS             1734..1913
                     /locus_tag="ACHXWD_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1940..2425)
                     /locus_tag="ACHXWD_003715"
     CDS             complement(1940..2425)
                     /locus_tag="ACHXWD_003715"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR7622507.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWLVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAADLRQ
                     K"
     gene            complement(2681..3661)
                     /locus_tag="ACHXWD_003716"
     CDS             complement(2681..3661)
                     /locus_tag="ACHXWD_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR7622508.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIHLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3661..4860)
                     /locus_tag="ACHXWD_003717"
     CDS             complement(3661..4860)
                     /locus_tag="ACHXWD_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7622509.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5034..5168)
                     /locus_tag="ACHXWD_003718"
                     /pseudo
     CDS             complement(5034..5168)
                     /locus_tag="ACHXWD_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5182..5535)
                     /locus_tag="ACHXWD_003719"
                     /pseudo
     CDS             complement(5182..5535)
                     /locus_tag="ACHXWD_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5602..>5683)
                     /locus_tag="ACHXWD_003720"
     CDS             complement(5602..>5683)
                     /locus_tag="ACHXWD_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622510.1"
                     /translation="FVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1569 a         1296 c         1333 g         1485 t
ORIGIN      
        1 agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag gaatgaggtg
       61 gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga agctgaacga
      121 aagtaaactg tccgatgaag catcgaaatc ttagatgatg tcaaaatggg gccgtacagc
      181 cgccccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg
      241 cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc
      301 gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg
      361 aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga
      421 tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc
      481 gtcgcaacaa gggagccccc ggatgcgacg ggcagacttt gaaaatgttc gaccagcaac
      541 gtgatggcaa tctatacaag atctggaacc ggctatgttc gggaacatgg tttccgccac
      601 cagtgctgga aaaacggatc ccgaagtcga atggcaagga acgcattctg gggatcccaa
      661 cagtatccga tcgaattgct cagggagcga taaaactttt catggaagaa aagcttgacc
      721 cgattttcca tgctgattca tatggttacc gaccaggcaa atcagctcat gacgctctga
      781 aacaatgtgc catccggtgc tggcgttata gctggatact ggaagtggac atcagtgcgt
      841 ttttcgacca tgtgagacat gatctggtgc tcaaagctct ggaacaccac gggatgccta
      901 aatgggtcat cctgtactgt cgacgctgga tggaagcgcc aatgcagagt tgtgaaaatg
      961 gagaattaat aacccgaaca cgaggcactc cgcagggcgg ggtcattagt ccgctacttg
     1021 ctaacttgtt ccatcacgat gcatttgatt tgtggatgga aagagaatat cgggggtacc
     1081 gtttgagagg tacgctgacg atattgtagt acattgttca cgaatgagtg atgcaacaag
     1141 gctgaagaac aggttatcgg agcgcttctc ggaagtcggg ctggtcctga atgcagggaa
     1201 aacgaatatc gcctacattg acacgtttaa aaggagaaac gtcgcaacga gtttcacctt
     1261 tctcggatat gacttcaaag tgcgtacgct gaagaatttc aaaggcgaac ggtaccgaaa
     1321 atgcatgccg ggtgcgtcaa atgcagcaat gcgcaaaata acagaaacaa tcaagaagtg
     1381 gcgtatacat cgctcaacag ctgagagttt gctggatttt gcgaggcgct acaatgcgat
     1441 agtgagaggc tggatcgagt actacggaaa gttctggtcc agaaatttca actatcgact
     1501 gtggagtgca atgcagtcac gtctgctcaa gtggatgcag tctaaataca gactttcgaa
     1561 ccggaaggct cagcgaaagc tgacgctggt aaggaaggag tatccgaagc tatttgttca
     1621 ctggtattta ctacgtgcat caaatgagtg atcaagagcc gtatgacgcg agagtgtcac
     1681 gtacggttcc gtgagcagcc ggggagtgaa attctctccg gctgactcga ctcgcgggtt
     1741 ccgacagcga tgacgaacat gcggcggtgt tgtactcgct gatcggcaca tgccgtctga
     1801 acaatgtgga accagaaaaa tggctgcgtt acgtcattga gcatatccag gactggctgg
     1861 caaaccgggt acgtgatctg ttgccctgga aagttgatct gagctctcag taaatatcaa
     1921 tacggttctg acgagccgct tacttttggc gtaaatctgc tgcgccttta ctgaacacaa
     1981 tgccgtattc agcaagaagt ccacgaatgt gatttatcag ttgtgtcctt tccattgaaa
     2041 ccaaccgacg catattcaaa aaagtctgga cgtcctgttg ctccagagtt tttattggta
     2101 cgggtcgtat agttccatgt tggcaggcct cagctattgc ctgtgcgtcg ttataatcat
     2161 ttttctggca ccgctgataa gctttaacat gttgtgccgg aagcaatatt acatcatggt
     2221 cgagtttcct gatttcccgg gcccagtaat gagatgcccc gccagcctcc atcgcaacag
     2281 tcattttagg ctgttgcacc agccagttaa gcagttgatt gcgagaaagt ttggtctttc
     2341 ctgcgggttt gccatgagca ttgatactga aaagataaaa gttcgtttta gccagatcaa
     2401 tgccggtgat tttctgttca gacatggtat tccgcttact tttgtggtta aagtttatcc
     2461 atcatggcgc aattgaaggc tgccgtgaaa gagggggaag actatctcat cggttactgt
     2521 taaacggcta agagaaacta tctttttcaa gcaaagaaga tacggtgaaa caggcgattt
     2581 caccgtgaaa tactgttcaa aaaaaacgct aataaaagca aaatttcttt taaaacagcc
     2641 aattaaaaac tggtgagttg ctggcgaaaa acttgaattt tcacagcgaa ttcagattat
     2701 cttttagtac aagagcaatc atacgatcta acttatcctg aacttccaac ggtaatctgc
     2761 cgaattcata tgagaacgta cgtcccttta ctcgttttcg agcaaattta tctttactat
     2821 caaatttcca taacaaagtt acaaccgctt tatcctttac tcctgaatca agcaatgaag
     2881 acatttcctt ggttataagc cgcataatac tgtttttaac ctcgtcttca gaaagattgt
     2941 catcagcagt aataagctca atactcggcg agatattctt cacaacttca tcaatagata
     3001 tatcacccag cctcagtcgt tcacttaatt cagccagttg cctgtaatcg gcgtatgtca
     3061 gctcagactg aacaggaaaa agagacacaa aatctttagg tacggacgct gcttggaggg
     3121 cacgagtcac cttagctgcc gacaatccct ctctttccgc aatttgcttc tggttcatac
     3181 cggcctcttt gagacgtact aaacgcaacc caatttcacg aatattatgt tcaagtgacg
     3241 tctgcaaatc tttcgccaga tgctgagctt cagaaacagt aagctcatca tccgttacaa
     3301 gtacacgtaa tccaaccttg catagcaaag ctgctgcccg acgacgggaa ccatccaaaa
     3361 tctcaataag atcgccagtc cgaatcccaa tacatggata gaattgttgt aaatgtatag
     3421 ttctggtgat gtcttttagt gagtccggcg tcagggctgt ctgttcgcgg ccattcacct
     3481 cttcaaccac aaaagtttga gtgtccactt tgtcatgatc aaccttgatc tcagtaaatt
     3541 ttgcctttcg gccagtattg agggtaaata catgaactgg agccgatatt tcttcagtat
     3601 tattcaggat atttgtattc agcgtacggc caatagtcgg tcggtgcttt ctgttttcca
     3661 tcagtagttg gcccttataa attcaatacg atcaaaaact gctttagcga aatcttctgc
     3721 tgccatacgt gcatttttca aagcttcacc actgccaaca taagtaacag gattcgcaga
     3781 tatcacggta tcaaacgact cgccgcttct ctcaaaacca tcaagacgtg gcattgaaac
     3841 atcaagcatg tcaccaccga aaatctcttt tgtcaggcta tggcagtatt tatgatctga
     3901 cttattcgcc agtttcgaca tgaagccaat atttgcctgc aaacggcatg aacatccatc
     3961 ctgttcaata atctgtacaa gttcaggtaa acgagccaaa tatttaagcg tggaatggaa
     4021 atcaacttgt gctggcggca ccggtgtaaa catgatatct gcggcagcaa tggcattttt
     4081 caaaaatgcg tccagatgcg ggcctgtatc aataaggatg aagtcgaaat catgcttcag
     4141 cttatcaata atattctcac gcaatatcgc gtgtttgttt tgtcctaata gatgctcctc
     4201 gcacagagta tcccagtttg atgcaataaa ggcgtcgtca atagaagccg gcataacata
     4261 aacgccaggg ataactgacg gaacgataaa atcttccagt agttcctcac gactcacgtt
     4321 ctgcaacata gcctgcggtg cggttgtatc aaccagcccc accgcatgca agtagttcag
     4381 aaacatagtg gctgatgact ggggatctaa atcaaggagc agaatacgta aatcttctga
     4441 cagcaggtga ggatgagcac gcagggcgtg agctacggaa acactactca cggttttaga
     4501 cacaccgcct tttagactac cgatgaatat ggaatatgcc tcactataac gatcgcgata
     4561 tttaggaatg ccacgatgtg catagatatc gataatgttc tggatggtta atgcatattt
     4621 tgtcgcggtt cctgctgggc gtttttcaaa ctgatagccc tgagcctcca tctcaccgac
     4681 tgcaccatcc acgcttgcgc gcgttaattt tggaagctta gctacagcag actttgaata
     4741 gacttgataa aaaacatcag cctgaaactc ctgcttttgc tcctgaatct gttttgttag
     4801 ggcgagcaac attttatctg cacgctgcgc aaccttactt aactgctcaa aactagtcat
     4861 tccccctccg tgctatgtag gattttaact tattacaaaa aaaactgcat tagcagggta
     4921 atgcatgcaa gtacatattg tacatgctta tattaaaaat gcgcgcattg ctgatttatt
     4981 aatcatcgga atgaatatta ctgtaagata gtatgccttt ccgggagaaa gacgctgatt
     5041 tcccccgaat aatccagttc accaccacac tccggacaac agctttcttc aggctccaga
     5101 tggtgtattt cgcgggaaac atgttccggt aacggacggc gatgacgtga ctgtcgcaac
     5161 aggcgggggt agtgcttcca atcagtagaa catgtgcttt tcaatgaagg agccgatgac
     5221 tttttcgtgg atttccacgg aacgcgagaa gcagattgtt ttacgtgcca gccgcttgat
     5281 gcgggtgcgg agggtccggt tgttacgttc aatgcgttgc gtgaagattt tgccggtcag
     5341 gtgtttctcc ttcggcactt ctctggcgta gctgccccag tcatcgctgg tcaccatgcc
     5401 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     5461 cgtggcccga aagtcgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     5521 ccagagccag tgttgtgtca acgacggatg aaaagtgatc cacttatatc tccaccaacg
     5581 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     5641 tttctgtctg agtcgatagc tttctccttt gatttgaacg aaa
//
LOCUS       DAZSBF010000243         5631 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZSBF010000243 DAZSBF010000000
VERSION     DAZSBF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.248"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..706
                     /locus_tag="ACHXWD_003721"
     CDS             <1..706
                     /locus_tag="ACHXWD_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7622511.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="ACHXWD_003722"
     CDS             complement(1909..2100)
                     /locus_tag="ACHXWD_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR7622512.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="ACHXWD_003723"
     CDS             2165..2488
                     /locus_tag="ACHXWD_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR7622513.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAQPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2697..3128
                     /locus_tag="ACHXWD_003724"
                     /pseudo
     CDS             2697..3128
                     /locus_tag="ACHXWD_003724"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="ACHXWD_003725"
     CDS             3166..3696
                     /locus_tag="ACHXWD_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR7622514.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="ACHXWD_003726"
     CDS             3703..3936
                     /locus_tag="ACHXWD_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR7622515.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="ACHXWD_003727"
     CDS             4417..5469
                     /locus_tag="ACHXWD_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622516.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5483..>5631
                     /locus_tag="ACHXWD_003728"
     CDS             5483..>5631
                     /locus_tag="ACHXWD_003728"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFR7622517.1"
                     /translation="MLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVILSWI
                     VLFIK"
BASE COUNT         1487 a         1153 c         1514 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg cacagccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAZSBF010000244         5620 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZSBF010000244 DAZSBF010000000
VERSION     DAZSBF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.249"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHXWD_003729"
     CDS             complement(<1..513)
                     /locus_tag="ACHXWD_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR7622518.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHXWD_003730"
     CDS             complement(759..1211)
                     /locus_tag="ACHXWD_003730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7622519.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHXWD_003731"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHXWD_003731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR7622520.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHXWD_003732"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHXWD_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR7622521.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHXWD_003733"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHXWD_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHXWD_003734"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHXWD_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR7622522.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHXWD_003735"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHXWD_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7622523.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZSBF010000245         5597 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZSBF010000245 DAZSBF010000000
VERSION     DAZSBF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.250"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            342..911
                     /locus_tag="ACHXWD_003736"
     CDS             342..911
                     /locus_tag="ACHXWD_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7622524.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            1639..1812
                     /locus_tag="ACHXWD_003737"
     CDS             1639..1812
                     /locus_tag="ACHXWD_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR7622525.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2070..3398)
                     /gene="rutG"
                     /locus_tag="ACHXWD_003738"
     CDS             complement(2070..3398)
                     /gene="rutG"
                     /locus_tag="ACHXWD_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR7622526.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3419..3913)
                     /gene="rutF"
                     /locus_tag="ACHXWD_003739"
     CDS             complement(3419..3913)
                     /gene="rutF"
                     /locus_tag="ACHXWD_003739"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR7622527.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(3924..4514)
                     /locus_tag="ACHXWD_003740"
     CDS             complement(3924..4514)
                     /locus_tag="ACHXWD_003740"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR7622528.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4524..5324)
                     /gene="rutD"
                     /locus_tag="ACHXWD_003741"
     CDS             complement(4524..5324)
                     /gene="rutD"
                     /locus_tag="ACHXWD_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR7622529.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5332..>5597)
                     /gene="rutC"
                     /locus_tag="ACHXWD_003742"
     CDS             complement(5332..>5597)
                     /gene="rutC"
                     /locus_tag="ACHXWD_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR7622530.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1481 a         1465 c         1390 g         1261 t
ORIGIN      
        1 cagaatctga aaatttttat aaaatcgtag tagcccctct tcaaattccc cttctaaaaa
       61 tcttcgaaaa tatctgacag atgtctgtag ctgctaagta cccgtcacac cagctatgtg
      121 accccctttg caaaaaagcc aaatatcatt gactttctcg gtgaattgct ataaaggcat
      181 ctatctaggt ctttaactgt ataaatacac atgacaacaa acgcccgtta agtgtctata
      241 taaaaaggta gctgtagcta tagacaacaa ttgtgataat tgatttaatg gctatagaca
      301 agcaagacag acctttgttg actatataga gagataagaa catgcgtaca ttcctttatg
      361 ctcgtgtatc agttgatgac ctcaccacac agaaccaagt agaagctgtt aaacgtgctg
      421 gttatgaagt cagaccttca cgagtaattg aggaagttat tagtggggct acaccagcta
      481 tggacagacc acaatttgta aagctggtgg ataagctgga agaaggtgat tgtcttgtag
      541 tgaccaagat tgaccgctta ggaagggata acattgacgt acagaagacc gtcacaatgt
      601 tacttggtat gggtgttaag ctggtctgtc tagatttgcc tgtccaagac ctttcaagtg
      661 ctgaaggtaa acttattctt caactattca gtacctttgc agagtttgaa cttaaccgca
      721 tcagagagcg aaccagagag ggcttagaac gagctaaagc acaaggtaag gtgttgggtc
      781 gtcctgtagc taaaaacact acagaggccg ttttagagca taaggctaag ggattgtcac
      841 aaagtcaggt tgcaaagctt ctgaatctct ctgtgagaac ggttagccgt cactggacaa
      901 agacactgta aaaaaaactt agtagcatct tcatagcctc cttcatgggg gcttttttgt
      961 gtctgtaaca tatggtgacc aaacatctat atatcgttat atagccctgt atatagcact
     1021 atatatagtg tctggagagg gacgactaac accctgcata gttccctgaa aaggggcttc
     1081 acagattctc tatatatttc aataagttag gataaaaagg gactataccg atactgcata
     1141 gaatctgtgt aggattgcat agtcataggg gttggaacaa gcccaactac acctgataaa
     1201 gcctgttatt aacaggtagt tgaatgtacg tgtttagcta tgtaaaagca ccagaacttt
     1261 agccatttct aaccactcct cgtgttatct ctattccgta gcgattcgct accacttatt
     1321 taaagataag acgtcctttt cagagtgcaa atttcacaac cacttatttg atttataaca
     1381 actttcacaa gcacgtaatt ttgtcgcaaa atgacacatt tttatctcat cgcgtttttt
     1441 taatcataag agcggcttat ggataattat tggagatgat atctattctc gctaagaagc
     1501 tgttgcagga tattaccaaa cgcgggtcta cccgcgtcag ttcactaagc ttagtcccac
     1561 gtagcgaaaa tatggcagcc gccatacgcc gcgttaattc tatgcaatat gatgtctata
     1621 cccagacgga ggtcagtaat ggcaaaccat cgaggcggtt ccggcaattt tgcagaagac
     1681 cgcgaaagag catcagaagc aggtaaaaaa ggtggacagc acagcggggg taatttcaag
     1741 aatgacccgc agcgcgcatc tgaagcaggt aaaaaaggtg gcaagagcag tcacggcaaa
     1801 agcgacaact agccgggcta atcaatgacg aatgcatttt tgtctgtagc tcgtcaaaaa
     1861 gccatcaccg ccggttaccc ggtggttgat actgatgaca aatgtaagct tgcctgatgc
     1921 gcgatgctta tcaggcctac cagaagattg caatatattg aatttgcact gttttgtagg
     1981 ccggataagg cgtttacgcc gcatccggca tgaacaatgc gtacgttgtc aacaatctgc
     2041 accgccgggt aaccccggcg gttttctgtt tatggctcct gatgaacaac ttctggcggt
     2101 ggaacgtcaa ccaaccttct acttaacaac gcattgagta aaatcgcgcc aaaggtggct
     2161 gtaccgatcc ctcccaacgt aaaaccgccc agcgtgagag caaaatcacc cgcgcccagc
     2221 actaaggtta ctgcgaccat aatcaaatta ccgttctggc ttaaatcgac acggttttgt
     2281 acccagatcc ttgcgcctgc gacggcaatc agcccgaaca caacaattga tgcaccacca
     2341 atcaccgcgg ccggaatggt atggatcagc gcgccaaatt tcggcgaaaa gcccaacagc
     2401 atggcgatga cggcagcagc aacaaacacc agcgtcgagt agacttttgt cacggccatc
     2461 acaccgatat tttcagcata ggtggtgacg ccgctaccgc cgacagagcc ggaaagcatc
     2521 gttgccagac catcaccgac gaatgctcgc cccatatacg ggtccatatt gcgtccggtc
     2581 atcccggcga ccgccttgag atgacctaag ttttccgcca ccagaatcac cgccacgggc
     2641 gcaatcagca tcattgcctg actattaaaa gcaggagtgg aaaaatgtgg caaaccgaac
     2701 caggcagcat ggctgacgag agtaaaatcg acggcttttc ccagccctaa aacgttggtc
     2761 atcacgccat acagcagaca ggcgacaatt aaacctacga gaatcaataa ccgctggatc
     2821 atgccacggg taaacaccgc caccagccca atacacagca ccgtcattac cgccatccag
     2881 ctatcaaagg ctgaagccga tacacttttc actgcgatag gcgctaagtt caggccaatc
     2941 gccatcacca ccgcacccgt caccaccggc ggcattagtc gttcaatcca gcgtgtacca
     3001 attttcatca ccaccaggcc aatgacggta taaaccagcc cacaggcgat aatcccgccc
     3061 agtgcgatgc tgatattcgg gttaatgccc tgaccgttaa agcccgtcgc ggcaatcacc
     3121 acgccgacaa aagccgcgct ggagccgaga taactgggga cgcgcccgcc ggtaataaag
     3181 aaaaacagta acgtgccgat ccccgacatt aaaatggaaa gattgggatc cagccccatc
     3241 agaatcggca ttaacaccgt cgcgccaaac atcgccaccg cgtgttgaac gcccattact
     3301 gccgtctgag caaacggcaa tcgttcatcc ggcgcgacca cgccgctctc tgtagaggtc
     3361 gattttaact gccagtgagg aaaaccgaac attgccatca gctgtctcct taaggaggtt
     3421 aacaagcagg gcgcatcagc gcgtgataac tgcgatcgaa ccacaccagc ccgtagggtg
     3481 tggtgtgacg atgaatcgct tcgatggcgc aaaacagaat gtcgtgggtg ccgacgctca
     3541 ccacctggct gatgcggcag tcaaacgaaa ccagagcctc ttccagttgc gggcatccgg
     3601 tcacacccgt ctgccagcgg gcggcggcaa agcggtgttc catgggcgtt ttgccgccaa
     3661 aaagatttga aagcggctcc tgcccggcgc tcagtgtatt tacacacagc gttcgatttt
     3721 cattgaatac cggccagacg gacgccccac gattcaggca caccagtaat gtgggcggcg
     3781 tgtcggtcac gctgcagacg gcgctggcgg tgaacccggc gcgcccggat ggaccgtccg
     3841 tggtgatgat attgaccgcc gcgcccatac aggacatcgc atcgcgaaag gtttgttgat
     3901 caactatgtt catagtttgc tccttacaac agcccgcagg cttcttcaaa ggacagacgt
     3961 ggcaggcgcg caaaaagttt gctgctatcg ccatagccga tattaatcag cagattgctc
     4021 ttcagcgtgc tgcccgcaaa aaaggcgtcg tcaacgtgtt gacggtcaaa acccgacatc
     4081 gggccggtat ccagtcccag cgcacggcaa gcgacgatca gataggccgc ctgcatggag
     4141 ctgttgcgaa acgctgtttc ttcggcaagt tgtgggctgg aggtaaacca actgcgggca
     4201 tcaccgtggg gaaacagtag tggtaaccgt tcataaaatt cactgtccca ggcaacgata
     4261 gcggtgacgg gcgcggtcag ggttttttgt agattgccgc tggaaagcgc cgggtgcaga
     4321 cgttcttttc cttctacagt gcggataaac acgatccgtg ccggagaaca gttagctgat
     4381 gtcggccccc attttatcgg ggcataaagc tcccgtaacg tctcatcgct gacgggtgtc
     4441 tcccgccagc cgttgtgagt gcgggcatcg gtgaacaggg tgctaagcgc acctgggcta
     4501 acggcttcgt tcatagcaat tccttacagg gcggcttcac ggtgatgtaa caggctggca
     4561 agcccgttga gtaacagagc attaaacgtt tcggggtcgg tcacgttgca ggcgtgtccg
     4621 ccatagcgca tcaccatttt ctggctatcg ggcagggcgg catgaagctc actggaacat
     4681 gctgatggca ccagcagatc atcactggcg cagatgattt gcaccgggca gcggatgcga
     4741 tccgcatggt gactaaagtc agcgcgtttg agggcgttaa gtcgacgcag taaattattt
     4801 ttgccctgaa aatgcgccag tgccagcgcg tcttctgcct caaggcgagg tgcgcgggcc
     4861 gccatccagt cggcgggata gaggaacaac ggctgcgctt ccacccatgc ctgcgcgccg
     4921 ccgctataca gtaatcgttc gcgaacctga aaacagcggc gcgtatgggc gtttattcgt
     4981 agccagccgt taacgctgat cagcacagtt accgacgcgg gataatccag cgccagctgc
     5041 attcccacca gcgcaccgag cgcatggccg accaccgcgt aatgctcaat ccctgcggct
     5101 accagcgcct gatgcagttc cgctgccatc tgggcgatac tgtaatcttc tgccagcgtg
     5161 tcgggattat tgccggtgcc gcgctggtcg taacagacta cctgatactc ctgctccagc
     5221 accgccagtt gcggtaacca gtaactgccg ctaccgccaa gacccgaaat caacaccact
     5281 acgggcgcat cagcataagg gggaggtgag agtgaaagtt tcatcgcggc ctcacttggc
     5341 gatatgcgca attgtggcga tttccaccag cgcgtcaggt tttaccagtc cgcactgaat
     5401 gcagaatcgc gccggtttat cacccggaaa aaactcggcg tagatttcgt taatcgcggc
     5461 gtaatttttc cagtcggtaa taaagatgct gttgaaggtc acatccgcca tcgtgccacc
     5521 cgccgtctcg atcaccttgc ggatagtttc cagaacgtgg cgggtttgcg cctttgggtc
     5581 atcggcaaac agcacgt
//
LOCUS       DAZSBF010000246         5527 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZSBF010000246 DAZSBF010000000
VERSION     DAZSBF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.251"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHXWD_003743"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHXWD_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR7622531.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHXWD_003744"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHXWD_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR7622532.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHXWD_003745"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHXWD_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR7622533.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHXWD_003746"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHXWD_003746"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR7622534.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHXWD_003747"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHXWD_003747"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR7622535.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHXWD_003748"
     CDS             4447..4812
                     /locus_tag="ACHXWD_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR7622536.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHXWD_003749"
     CDS             4962..5480
                     /locus_tag="ACHXWD_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR7622537.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1520 a         1391 c         1240 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZSBF010000247         5526 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZSBF010000247 DAZSBF010000000
VERSION     DAZSBF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.252"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHXWD_003750"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHXWD_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR7622538.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHXWD_003751"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHXWD_003751"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR7622539.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHXWD_003752"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHXWD_003752"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR7622540.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHXWD_003753"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHXWD_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR7622541.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1361 a         1325 c         1503 g         1337 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccg
//
LOCUS       DAZSBF010000248         5421 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZSBF010000248 DAZSBF010000000
VERSION     DAZSBF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.253"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            444..530
                     /gene="leuL"
                     /locus_tag="ACHXWD_003754"
     CDS             444..530
                     /gene="leuL"
                     /locus_tag="ACHXWD_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR7622542.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            623..2194
                     /gene="leuA"
                     /locus_tag="ACHXWD_003755"
     CDS             623..2194
                     /gene="leuA"
                     /locus_tag="ACHXWD_003755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR7622543.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDITTAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2194..3285
                     /gene="leuB"
                     /locus_tag="ACHXWD_003756"
     CDS             2194..3285
                     /gene="leuB"
                     /locus_tag="ACHXWD_003756"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR7622544.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3288..4688
                     /gene="leuC"
                     /locus_tag="ACHXWD_003757"
     CDS             3288..4688
                     /gene="leuC"
                     /locus_tag="ACHXWD_003757"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR7622545.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4699..5304
                     /gene="leuD"
                     /locus_tag="ACHXWD_003758"
     CDS             4699..5304
                     /gene="leuD"
                     /locus_tag="ACHXWD_003758"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR7622546.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5353..>5421)
                     /locus_tag="ACHXWD_003759"
     CDS             complement(5353..>5421)
                     /locus_tag="ACHXWD_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622547.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1406 a         1421 c         1402 g         1192 t
ORIGIN      
        1 aacaatctaa tcataactac cgcgaatact caatcatcta caaaatggat taaatgtgct
       61 tatttaataa ttaatttata tatttaatgc attaattctt aacattaatt gatcaataat
      121 attcaccaaa tcaatatcaa aaaaaatcgc aaaacatata attcaataca aatcatcagg
      181 ataggttttg caacgcgtgc attttgtccc ctttttcctc gttgattaga tgcaaaaatt
      241 tatgctgaaa tatgtcaacc gatgaaaagc gtcggtagtt aagcagaaat taatatcgct
      301 tactttaacc accgcagcac aattagctaa ttttacggat gcagaactca cgctggcggg
      361 acgtttttat tgcgtcaggg ttgacatccg tttttgtatc cagtaactct aaaagcatat
      421 cgcattcatc tggagctgat ttaatgactc acatcgttcg ctttatcggt ctactactac
      481 taaacgcatc ttctttgcgc ggtagacgag tgagcggcat ccagcattaa gccagcacgc
      541 agtcaaacaa aaaacccgcg ccattgcgcg ggttttttta tgcccgaagc gaggcgctct
      601 aaaagagaca aggacctaaa ccatgagcca gcaagtcatt attttcgata ccacattgcg
      661 cgacggtgaa caggcgttac aggcaagctt gagtgtgaaa gaaaaactgc aaattgcgct
      721 ggcccttgag cgtatgggtg ttgacgtgat ggaagtcggt ttccccgtct cttcgccggg
      781 cgattttgaa tcggtgcaaa ccatcgcccg ccaggttaaa aacagccgcg tatgtgcgtt
      841 agctcgctgc gtggaaaaag atatcgacgt ggcggccgaa tccctgaaag tcgccgaagc
      901 cttccgtatt cataccttta ttgctacctc cccaatgcac atcgccacca agctgcgcag
      961 caccctggac gaggtgatcg aacgcgctat ctatatggtg aaacgcgccc gtaattacac
     1021 cgatgatgtt gaattttctt gcgaagatgc cgggcgtaca cccattgccg atctggcgcg
     1081 agtggtcgaa gcggcgatta atgccggtgc caccaccatc aacattccgg acaccgtggg
     1141 ctacaccatg ccgtttgagt tcgccggaat catcagcggc ctgtatgaac gcgtacctaa
     1201 catcgacaaa gccattattt ccgtacatac ccacgacgat ttaggcctgg cggtcggcaa
     1261 ctcactggcg gcggtacatg ccggtgcacg ccaggtggaa ggtgcaatga acgggatcgg
     1321 cgagcgtgcc ggtaactgtt cgctggaaga agtcatcatg gcgattaaag ttcgtaagga
     1381 tattctcaac gtccacaccg ccattaatca ccaggagata tggcgcacca gccagttagt
     1441 tagccagatt tgtaatatgc cgatcccggc aaacaaagcc attgttggca gcagcgcatt
     1501 cgctcactcc tccggtatcc accaggatgg cgtgctgaaa aaccgcgaaa actacgaaat
     1561 catgacacca gaatctattg gtctgaacca aatccagctg aatctgacct ctcgttcggg
     1621 gcgtgcggcg gtgaaacatc gcatggatga gatggggtat aaagaaagtg aatataattt
     1681 agacaatttg tacgatgctt tcctgaagct ggcggacaaa aaaggtcagg tgtttgatta
     1741 cgatctggag gcgctggcct tcatcggtaa gcagcaagaa gagccggagc atttccgtct
     1801 ggattacttc agcgtgcagt ctggctctaa cgatatcacc accgccgccg tcaaactggc
     1861 ctgtggcgaa gaagtcaaag cagaagccgc caacggtaac ggtccggtcg atgccgtcta
     1921 tcaggcaatt aaccgcatca ctgactataa cgtcgaactg gtgaaataca gcctgaccgc
     1981 caaaggtcac ggtaaagatg cgctgggtca ggtggatatt gtcgccaact acaacggtcg
     2041 ccgcttccac ggcgtcggcc tggccaccga tattgtcgag tcctccgcca aagccatggt
     2101 gcacgtactg aacaatatct ggcgtgccgc agaagtcgaa aaagagttgc aacgcaaagc
     2161 tcaacacaac gaaaacaaca aggaaaccgt gtgatgtcga agaattacca tattgccgta
     2221 ttgccggggg acggtattgg tccggaagtg atgacccagg cgctgaaagt gctgaatgcc
     2281 gtgcgcaacc gctttgcgat gcgcatcacc accagccatt acgatgtagg cggcgcagcc
     2341 attgataacc acgggcaacc actgccgcct gcgacggttg aaggttgtga gcaagccgat
     2401 gccgtgctgt ttggctcggt aggcggcccg aagtgggaac atttaccacc agaccagcaa
     2461 ccagaacgcg gcgcgctgct gcctctgcgt aagcgcttca aattattcag caacctgcgc
     2521 ccggcaaaac tgtatcaggg gctggaagca ttctgtccgc tgcgtgcaga cattgccgct
     2581 aacggcttcg acatcctgtg cgtgcgcgaa ctgaccggcg gcatctattt cggtcagcca
     2641 aaaggccgcg aaggtagcgg acaatatgaa aaagcgtttg ataccgaggt gtatcaccgt
     2701 tttgagatcg agcgtattgc ccgcatcgcg tttgaatctg ctcgtaaacg tcgccacaaa
     2761 gtcacctcaa tcgacaaagc taacgtgctg caatcctcta ttttatggcg tgaaatcgtc
     2821 aacgagatcg ccacggaata cccggatgtc gaactggcgc atatgtacat cgacaacgcc
     2881 accatgcagc tgattaaaga tccatcacag tttgacgttc tgctgtgctc caacctgttt
     2941 ggcgacattc tgtctgacga gtgcgcaatg atcactggct cgatggggat gttgccttcc
     3001 gccagcctga acgagcaagg ttttggtctg tatgaaccgg caggcggctc agcaccagat
     3061 atcgcaggca aaaacatcgc caacccgatt gcgcaaattc tgtcgctggc actgctgctg
     3121 cgctacagcc tggatgccga tgatgcggct tgcgccattg aacgcgccat taaccgcgca
     3181 ttagaagaag gcattcgcac cggagattta gcccgtggcg ctgccgccgt tagtaccgat
     3241 gaaatgggcg atatcattgc ccgctatgtg gcagaagggg tgtaatcatg gctaaaacgt
     3301 tatacgaaaa attattcgac gctcacgtag tgtacgaagc cgaaaacgaa accccgctgt
     3361 tatatatcga ccgccacctg gtgcatgaag tgacctcacc gcaggcgttc aatggtctgc
     3421 gcgcccacgg tcgcccggta cgtcagccgg gcaaaacctt cgccactatg gatcacaacg
     3481 tctctaccca gacgaaagac attaatgcct gtggtgaaat ggcgcgcatc cagatgcagg
     3541 agctgattaa aaactgcaaa gaatttggcg tcgaactgta tgacctgaat cacccgtatc
     3601 aggggatcgt ccacgtaatg gggccggaac agggcgtcac cttgccgggg atgaccattg
     3661 tctgcggcga ctcgcatacc gccacccacg gcgcgtttgg cgcactggcc tttggtatcg
     3721 gcacttccga agttgaacac gtactggcaa cgcaaaccct gaaacagggc cgcgcaaaaa
     3781 ccatgaaaat tgaagtccag ggcaaagccg cgccgggcat taccgcaaaa gatatcgtgc
     3841 tggcaattat cggtaaaacc ggtagcgcag gcggcaccgg gcatgtggtg gagttttgcg
     3901 gcgaagcaat ccgtgattta agcatggaag gtcgtatgac cctgtgcaat atggcaatcg
     3961 aaatgggcgc aaaaaccggt ctggttgcac cggacgaaac cacctttaac tatgtcaaag
     4021 gccgtttgca tgcgccgaaa ggcaaagatt tcgacgacgc cgttgcctac tggaaaaccc
     4081 tgcaaaccga cgaaggcgca actttcgata ccgttgtcac tctgcaagca gaagaaattt
     4141 caccgcaggt cacctggggc accaatccag gccaggtgat ttccgtgaac gacaatattc
     4201 ccgatccggc ttcgtttgcc gatccggtta aacgcgcgtc ggcagaaaaa gcgctggcct
     4261 atatggggct gaaaccgggc attccgctga ccgaagtggc tatcgacaaa gtgtttatcg
     4321 gttcctgtac caactcgcgc attgaagatt tacgcgcggc agcggagatc gccaaagggc
     4381 gaaaagtcgc gccaggcgtg caggcactgg tggttcccgg ctctggcccg gtaaaagccc
     4441 aggcggaagc ggaaggtctg gataaaatct ttattgaagc cggttttgaa tggcgcttgc
     4501 ctggctgctc aatgtgtctg gcgatgaaca acgaccgtct gaatccgggc gaacgttgtg
     4561 catccaccag caaccgtaac tttgaaggcc gccaggggcg cggcgggcgc acgcatctgg
     4621 tcagcccggc aatggctgcc gctgctgctg tgaccggaca tttcgccgac attcgcaaca
     4681 ttaaataagg agcacaccat ggcagagaaa tttatcaaac acacaggcct ggtggttccg
     4741 ctggatgccg ccaatgtcga taccgatgca atcatcccga aacagttttt gcagaaagtg
     4801 acccgtacgg gttttggcgc gcatctgttt aacgactggc gttttctcga tgaaaaaggc
     4861 caacagccaa acccggactt cgtgctgaac ttcccgcagt atcagggcgc ttccattttg
     4921 ctggcacgag aaaacttcgg ctgtggctcc tcgcgtgagc acgcgccctg ggcattgacc
     4981 gactacggct ttaaagtggt gattgcgccg agttttgctg acatcttcta cggcaatagc
     5041 tttaacaacc agctgctgcc ggtgaaatta agcgatgcag aagtggacga actgtttgcg
     5101 ctggtgaaag ccaatccggg gatccatttc gacgtggatc tggaagcgca agaggtgaaa
     5161 gcgggagaga aaacctatcg ctttaccatc gatgccttcc gccgccactg catgatgaac
     5221 ggtctggaca gtattgggct taccttgcag cacgacgacg ccattgccgc ttatgaagca
     5281 aaacaacctg cgtttatgaa ttaatcccct tgcccggtca aatgaccggg ctttccgcta
     5341 tcgtccacgt catcaaacgt ctttaacctt tgcggttaaa aataatgcga caacagaaat
     5401 aaccgcaatt acccagtaga c
//
LOCUS       DAZSBF010000249         5413 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZSBF010000249 DAZSBF010000000
VERSION     DAZSBF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.254"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            93..1226
                     /gene="wzz(fepE)"
                     /locus_tag="ACHXWD_003760"
     CDS             93..1226
                     /gene="wzz(fepE)"
                     /locus_tag="ACHXWD_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR7622548.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(1223..2038)
                     /gene="fepC"
                     /locus_tag="ACHXWD_003761"
     CDS             complement(1223..2038)
                     /gene="fepC"
                     /locus_tag="ACHXWD_003761"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR7622549.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(2035..3027)
                     /gene="fepG"
                     /locus_tag="ACHXWD_003762"
     CDS             complement(2035..3027)
                     /gene="fepG"
                     /locus_tag="ACHXWD_003762"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR7622550.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(3024..4028)
                     /gene="fepD"
                     /locus_tag="ACHXWD_003763"
     CDS             complement(3024..4028)
                     /gene="fepD"
                     /locus_tag="ACHXWD_003763"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR7622551.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4139..5389
                     /gene="entS"
                     /locus_tag="ACHXWD_003764"
     CDS             4139..5389
                     /gene="entS"
                     /locus_tag="ACHXWD_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR7622552.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
BASE COUNT         1268 a         1451 c         1490 g         1204 t
ORIGIN      
        1 tacaaacttc agcttcggat aattctcacc aactggtaaa gtgagcgccg tggagatttt
       61 cccctattgg actcattttc aggttatacc ccatgtcatc attgaatatt aaacagggaa
      121 gtgacgctca tttttctgag tatcctctgg cgtcgcccag taataatgaa attgatttac
      181 ttaatctaat cgaagtttta tggcgggcca aaaaaacggt catggcggtc gtttttgcat
      241 ttgcctgcgc aggtttactg atctctttca tcctgccgca aaaatggacc agctcagccg
      301 ttattactcc cgccgaagcc attcagtggc aggacctgga gaaaaccttt accaaactcc
      361 gcgttcttga tctcgatgtt aatatcgacc gtggcggtgc atttaacctg tttatcaaga
      421 agtttcagtc tgttagcttg ctggaagagt acctgcgttc atcaccttat gtgatggacc
      481 aattaaaaga ggcgaaaatc gatgaactgg atttgcatcg cgctattgtt gcattgagcg
      541 aaaaaatgaa agcggttgat gacaatgcca gtaagaaaaa agatgaaccg tcactgtata
      601 cctcctggac gctaagtttt accgcgccaa ccagtaaaga ggcgcagacc gttttgagcg
      661 ggtatatcga ttatatctct gcgttggtgg tgaaagagtc gatagaaaac gtccgtaata
      721 aactggagat caaaacccag tttgaaaaag aaaaactggc tcaggatcgc attaaaacga
      781 aaaatcaact tgatgcaaac attcagcgcc tcaattattc actcgacatt gccaacgcgg
      841 caggaattaa aaagcccgta tacagtaatg gtcaggccgt taaagatgac cccgatttct
      901 ctatttctct cggcgcagac ggtattgaac gcaaactgga aattgaaaaa gcggttactg
      961 acgttgcgga actgaacggt gaattacgta atcggcagta tcttgtcgag caattaacaa
     1021 aagcaaatgt caacgatgtg aattttacgc cgtttaaata tcagttaagc ccgtcattgc
     1081 cagtgaaaaa agacggtccg ggtaaggcga ttattgtgat cctttccgcg ctgatcggcg
     1141 ggatggtggc ttgtggtagc gtgttattgc gctatgcgat ggcatccaga aaacaggatg
     1201 ccatgatggc agaccactta gtttaactat ttgcggttga aggtgccgtt cgtccgagcg
     1261 gcaccacaag cggcgttccg gctaccggat cgtcaatgat catgcatcgc agaccataaa
     1321 tgcgctcaat cagttcagca gtgacaatct ccttcggcgc tccctgagca acaattttcc
     1381 cttcccgcaa tgcaatcaaa tggctggcgt aacgacaggc ctgattaaga tcgtgcagca
     1441 ccgccgccag ggtatagcct ttctcgcggt tcagttcgct taacagttcc agtagatcaa
     1501 tctgatgact gatatccagc caggtcgtgg gttcgtcgag cagcataatt gccgtttcct
     1561 gggctagcac catcgcgatc cacgctcgct ggcgttgtcc gccagaaagg gtatccacgc
     1621 tttgatctgc cagatgagtt attctcgtgg cctgcatcgc tttcgttacc gcttcttcat
     1681 cctctttgcg ccagcgggta aacagcggtt gatgcggata acgtccacgc gccaccagct
     1741 cctgcacggt gatatcgccc ggcgtggtag cattttgcgc caacagacca atccggcgtg
     1801 caacttcttt actggcgtaa tgttgaatgt gctcgccatc cagccagaca tgcccgtgag
     1861 caggcgtcat caggcggctt aaggtacgca gtaacgtgga tttaccacag ccatttggtc
     1921 cgataattgc cgtgaagtga ccatcaggaa tttctacagt cagattttcc gcaacggtat
     1981 atttgccata tcccagggtt aactgttcgc cgcgcaaacg ggctactgat tcggtcattt
     2041 tttgcgagac tcctgaatta acaagacgat aaggtaaata ccgccgaggc tgacggtaac
     2101 gacgccaacc ggaagttgat acggcataaa cagttgttgg gcgcagagat cggccgccag
     2161 cagtaacagc gctccgcaca gcgccgcctg ggtcagcccc cagcgagcgg tgccgctaat
     2221 gcgtcgggca atgtgcggcg cgactaaagc aataaaggaa atcggcccgg caagcgccgt
     2281 tgccgcagcg gtaagcacca ctgcaaccag catcattaac agacgcgaac gctcgacgct
     2341 gacgcccagc gcgcaggcgg tatcatcgcc catctccagc aagcgcattc gccgcaccag
     2401 caaggcggcg gcaatgagca tcaatatgat gatgggtgcg gaaggcgaag tttttgccca
     2461 cgtcaggcca ttgagcgatc cggcattcca caaacccgct gttagcgccg tttctaaaga
     2521 cgctttcagc aacagccaag tattaaaggc caccagcatg gcgcgaacgc cgatgccgat
     2581 aataatcaac cgaaaggtgt cgataccgtt acgccaggcg agcagccaga ccaacagaga
     2641 agtgacaatg ccgcccacca ttgctgccag cgcgatagcc gtcaggtcct gaccaaacag
     2701 caccatcgcc accagcacgc cgctccacgc cccggtgtta aagcccatta cgtcagggct
     2761 gccgagcggg ttacgcatca gcgactgaaa aatcgcgccg ctgacgccca gcgccgcgcc
     2821 aatcaacagc gccatcagca cgcgtggtaa acgccattcg gtgaccacca tcgtcatact
     2881 gcgcggcgca tcgcccatca acgcggcgaa tacctgcgag atttccagcg tgacggcacc
     2941 gctgcgtaat ccccagatac ctgcaaccac acaggcggaa atcagcagca aacaggtgat
     3001 gagtaatcgg cgagagacgt aaatcatgca ccacctcgcg ttttacgtcg cacgaggaag
     3061 atcagcaccg gtgcaccaat aaacgcactg accacagaaa cgcgcagttc gccgggcaca
     3121 atcacccgcc cgatgatatc ggcaaacagc agcagggcag gggtagcaag tagcgtgacg
     3181 ggcagcgacc agcgatgatc ggcacctacc agccagcgcg ccatgtgcgg catcatcagg
     3241 ccgataaagg caatcgggcc aactaccgcc gtcgcactac cacaaagcac ggtaatcgcc
     3301 agcagaccaa tcaactgtgt gcgcgccacg cgactgccca gcgccgtcgc ggtgtcactg
     3361 ccgaggctca aactgttcag cgcgcgactc agtaatagcg cagttgctcc ggcgatcaga
     3421 accgggatca gcaccacttt taaggtatgt agattgcgaa tatccagcga accggcttgc
     3481 cagaaacgca gttgatcgta gacgtcagga ttaagcaggg cgatgccgct ggtcagccct
     3541 tccagcaccg ccgccagcgc cacgcccgcc agggttaaac gcaccggact taactgcccg
     3601 ccgccctgac tgccgataaa ggcaacaatc aatgaggcca ccagcgcccc ggcgaaggcc
     3661 atcgccagtt gttcctgcgc ggatgagtaa ccaaacagcg ccgcacccag cacaatggca
     3721 aagctggctc cggcgttcac gccaagcaag ccggggtcgg caagtggatt tcgggtgagg
     3781 gtttgcatta acgccccagc aagcccaagc gcaccgcctg caagtaaacc ggcaagggtg
     3841 cgcggcagtc gcgcgtcgag cacgatggtg cagtcggcgc tctggcaggt gcctgagaag
     3901 gcctccagca ctactgacgc ggggagtgat tttgccccaa tgagcaggct taatgccgtc
     3961 gcgataatca gtaataacag caatccgggc acggcaatgg cgcgtgtcac ggcaacagaa
     4021 ccagacatac gaacttccat gataatgaaa ttaattatcg ttatcgatct tatttggata
     4081 tgttagcatg tgcagcctaa gaataggtat ttaaaatatt tgatggcaag gcattgtaat
     4141 gaataaacaa tcctggctgc ttaacctcag cctgttgaaa acgcacccgg cgtttcgcgc
     4201 agtattcctc gctcgtttta tctcaattgt gtctctgggt ttgctcggcg tcgcggtgcc
     4261 ggtgcagatc cagatgatga cgcattctac ctggcaggtg gggctttcgg tgacgctgac
     4321 cggcggcgcg atgtttgttg gcctgatggt tggcggtgtg ctggcggatc gctatgagcg
     4381 caaaaaagtg attttgctgg cgcgcggcac ctgtggcatt ggcttcattg gactgtgcct
     4441 gaacgcgctg ctgccggagc cgtcattgct ggcaatctat ttacttggtt tatgggatgg
     4501 ttttttcgca tcacttggtg ttacggcgct actggcggca acacctgcac tggtagggcg
     4561 tgaaaactta atgcaggccg gggcgatcac catgttgacc gtgcgtctgg ggtcggtgat
     4621 ttcgcccatg attggcggtt tattgctggc gaccggcggc gtagcctgga actacgggct
     4681 ggcggcggcg ggcacgttta ttaccttgct accgttgtta agccttccgg cgttgccacc
     4741 gccaccgcag ccgcgcgagc atccgttgaa atcattactg gcaggatttc gttttctgct
     4801 cgccagcccg ctggtaggag ggattgcgtt gctgggtggt ttattgacga tggcgagcgc
     4861 ggtgcgggta ctgtatccgg cgctggctga caactggcag atgtcggcgg cacagattgg
     4921 ttttctctat gcggcgatcc cgctcggtgc ggctattggt gcgttaacca gcgggaagct
     4981 ggcacatagt gcgcgaccag ggttattgat gctgctctcc acgctgggat cgttcctcgc
     5041 cattggtctg tttggcctga tgccgatgtg gattttaggc gtggtttgtc tggcgctgtt
     5101 cggctggctg agcgcggtta gttcgttgct gcaatataca atgctgcaaa cgcaaacccc
     5161 ggaagcgatg ttagggcgga ttaacggttt gtggacggcg cagaacgtga cgggcgatgc
     5221 cataggcgcg gcgctgttgg gtggtttggg cgcgatgatg acaccggttg cttccgcaag
     5281 cgcgagcggt tttggtttgt tgattatcgg cgtgttgtta ttgctggtgc tggtggagtt
     5341 gcgacgtttt cgccagacgc cgccgcaggt gacagcgtct gacagttagc taaaggtgca
     5401 ttttcccctc acc
//
LOCUS       DAZSBF010000250         5384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZSBF010000250 DAZSBF010000000
VERSION     DAZSBF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.255"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(183..326)
                     /gene="ypaB"
                     /locus_tag="ACHXWD_003765"
     CDS             complement(183..326)
                     /gene="ypaB"
                     /locus_tag="ACHXWD_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR7622553.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            454..2739
                     /gene="nrdA"
                     /locus_tag="ACHXWD_003766"
     CDS             454..2739
                     /gene="nrdA"
                     /locus_tag="ACHXWD_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR7622554.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2973..4103
                     /gene="nrdB"
                     /locus_tag="ACHXWD_003767"
     CDS             2973..4103
                     /gene="nrdB"
                     /locus_tag="ACHXWD_003767"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR7622555.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4103..4357
                     /gene="yfaE"
                     /locus_tag="ACHXWD_003768"
     CDS             4103..4357
                     /gene="yfaE"
                     /locus_tag="ACHXWD_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR7622556.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4411..5060)
                     /gene="inaA"
                     /locus_tag="ACHXWD_003769"
                     /pseudo
     CDS             complement(4411..5060)
                     /gene="inaA"
                     /locus_tag="ACHXWD_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(5140..5244)
                     /locus_tag="ACHXWD_003770"
                     /pseudo
     CDS             complement(5140..5244)
                     /locus_tag="ACHXWD_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5239..5384
                     /locus_tag="ACHXWD_003771"
                     /pseudo
     CDS             5239..5384
                     /locus_tag="ACHXWD_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1347 a         1437 c         1305 g         1295 t
ORIGIN      
        1 tttaacaata accgaataga aaacaaccat ttcgacatca acaatcgctt acattcgctt
       61 atatattgac cacaactgat acatcagatt atgtgatgac tcgtgcttag atcaattttt
      121 gcaatcatta acaaaaagat taataaacca tctatatcaa tttatccaac ctattctgcc
      181 gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat tgactttccc
      241 ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga gttatccaca
      301 aagttatgca cttgcaagag ggtcattttc acactatctt gcagtgaatc ccaaacatac
      361 cccctatata tagtgttcta agcagcttcc cttactacag gtagtctgca tgaaactatt
      421 gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct ggtgacaaag
      481 cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct ggattgggcg
      541 gcagaaggac tgcataacgt ttcgatttcc caggtcgagc tgcgctccca cattcagttt
      601 tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc cgcagacctg
      661 atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcgat cttccacctg
      721 cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccacgt ggtgaaaatg
      781 gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga agagttcaag
      841 cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc tgccgttaag
      901 cagctggaag gcaaatatct ggtacagaac cgcgtgaccg gcgaaatcta tgagagcgcc
      961 cagttccttt atattctggt tgccgcgtgc ctgttctcga actacccgcg tgaaacgcgc
     1021 ctgcaatatg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc gctgccgacg
     1081 ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt actgatcgag
     1141 tgcggtgaca gcctggattc catcaacgcc acctccagcg cgattgttaa atacgtttcc
     1201 cagcgtgccg ggatcggcat caacgccggg cgtattcgtg cgctgggtag cccgattcgc
     1261 ggtggtgaag cgttccatac cggctgcatt ccgttctaca aacatttcca gacagcggtg
     1321 aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta cccgatgtgg
     1381 catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggtgtgga aggcaaccgc
     1441 gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg tctgctgaaa
     1501 ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga cgcgttcttc
     1561 gccgatcagg aagagtttga acgtctgtat accaaatatg agaaagacga cagcatccgc
     1621 aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg tgcgtctacc
     1681 ggtcgtatct atattcagaa cgttgaccac tgcaataccc atagcccgtt tgatccggcc
     1741 atcgcgccag tgcgtcagtc taacctgtgc ctggagatag ccctgccgac caaaccgctg
     1801 aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc tttcaacctg
     1861 ggcgcaatta ataacctgga tgaactggaa gagctggcaa ttctggcggt tcgtgcactt
     1921 gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacgtgg agcgatgggt
     1981 cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa gcacggtaaa
     2041 cgctactccg acggcagcgc caacaacctg acgcataaaa ccttcgaagc cattcagtat
     2101 tacctgctga aagcctctaa tgagctggcg aaagagcaag gcgcgtgccc gtggtttaac
     2161 gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga tctggatacc
     2221 atcgctaatg agccgctgca ttacgactgg gaagcattgc gtgaatcaat caaaacgcac
     2281 ggcctgcgta actctacgct ttctgccctg atgccgtccg agacctcttc gcagatctct
     2341 aacgccacta acggtattga accgccgcgc ggttacgtca gcatcaaagc gtcgaaagac
     2401 ggtattttgc gccaggtggt gccggactac gagcacctgc acgacgccta tgagctgctg
     2461 tgggaaatgc cgggtaacga tggctacctg caactggtag gtatcatgca gaaatttatc
     2521 gatcagtcga tctctgctaa caccaactac gatccgtcac gtttcccgtc aggaaaagtg
     2581 ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt caaaacgctg
     2641 tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt accgtcaatc
     2701 caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc cggatgcggc
     2761 gtaaacgcct tatccggcct acggctcggt ttgtaggcct gataagacgc gccagcgtcg
     2821 catcaggctc cgggtgccgg atgcagcgtg aacgccttat ccggcctacg gctcggattt
     2881 gtaggcctga taagacgcgc cagcgtcgca tcaggcacag gatgcggcgt aaaatgcctt
     2941 atccggcatt aaactcccaa caggacacac tcatggcata taccaccttt tcacagacga
     3001 aaaatgatca gctcaaagaa ccgatgttct ttggtcagcc ggtcaacgtg gctcgctacg
     3061 atcagcaaaa atatgacatc ttcgaaaagc tgatcgaaaa gcagctctct ttcttctggc
     3121 gtccggaaga agttgacgtc tcccgcgacc gtatagatta ccaggcgctg ccggagcacg
     3181 aaaaacacat ctttatcagc aacctgaaat atcagacgct gctggattcc attcagggtc
     3241 gtagcccgaa cgtggcgcta ttgccgctta tttctattcc ggaactggaa acctgggtcg
     3301 aaacctgggc gttctcagaa acgattcatt cccgttccta tactcatatc attcgtaata
     3361 tcgttaacga tccgtctgtt gtgtttgacg atatcgtcac caacgagcag atccagaaac
     3421 gtgcggaagg gatctccagc tattacgatg aactgatcga aatgaccagc tactggcacc
     3481 ttctgggcga aggcacccac accgttaacg gtaaaaccgt aaccgttagc ctgcgcgagc
     3541 tgaagaaaaa actgtatctc tgcctgatga gcgttaacgc gctggaagcg atccgcttct
     3601 acgtcagctt tgcttgttcc tttgcctttg cagaacgcga actgatggaa ggtaacgcca
     3661 agattattcg cctgattgcc cgcgatgaag ccctgcacct gaccggtact cagcatatgc
     3721 tgaatctgct gcgcagcggc gcggatgatc cggagatggc ggaaattgcc gaagaatgta
     3781 agcaggagtg ctatgacctg tttgtccagg cggcacagca agagaaagac tgggcggatt
     3841 atctgttccg tgacggttcg atgattggtc tgaataaaga tattctctgc cagtacgttg
     3901 aatacatcac caatatccgc atgcaggcag tcggtttgga tctgccgttc cagacgcgct
     3961 ccaacccgat cccgtggatc aacacctggc tggtgtctga taacgtgcag gttgctccgc
     4021 aggaagtgga agtcagttct tatctggtcg ggcagattga cgcggaagtg gacaccgacg
     4081 atttgagtaa cttccagctc tgatggcccg cgttaccctg cgcatcactg gcacacaact
     4141 gctgtgccag gatgaacacc cttcccttct ggcggcgctg gaatcccaca atgttgcggt
     4201 tgagtaccag tgccgcgaag gttactgcgg ctcctgtcgc acccgactgg tcgcaggtca
     4261 ggtggactgg attgccgaac cgttagcctt tattcagccg ggggaaattt tgccctgttg
     4321 ttgccgggca aaaggcgata ttgaaatcga gatgtgaatt ggttgtagtg ccagatacaa
     4381 cgcttatacg tcttatctgg cctacaacga ttacatggcg tagtaatacg ctttcacctg
     4441 ctcccagtcc gctttgggga ttggctccag atattttcca gctggcggaa gtcatgatta
     4501 atcgctttat tgcgacgcaa gcggcgacga cttttctcca ggtcaagaaa accagcttct
     4561 gctttacctt ctgttttcac atagatatgg cgaacataac aacagccatg ctgacgatta
     4621 acgctatgca ttttcttaaa cgccagcgcc actgctttca acatggcttg ccgtacttcg
     4681 tcagaatagg gcgatactgc atgccgggca taccagtcag caatgctgat gaaccccgcc
     4741 atatcttcag tcaccagcaa cgcgcgccat tcaccctcaa ttttcaccgc ttcaccaaaa
     4801 acgatcttcg gcacgatgac accggcccgt tccagttctt taattaccgc aacctcacgg
     4861 acaatcgttg gtcggccgaa cggataacgt acggaatgaa acagatgatg cgtcatgcgc
     4921 tttacataca gctttttgcc gttgcgctcg acgcattgca ccccgctcat accattacgg
     4981 cgatagttag gctcttcaac ccagtcgccc tctgttgccc accagtgatt aaattcgtcg
     5041 tactttgctg aaactgccat accctatcgc ctgtcatttt tattaacgac aatgactata
     5101 ggtggttacc tgaggaaaat cttaatgaaa cgtgtcgtat taatgaattt cttttgccgc
     5161 tactagtgtg acaccgcttt tgcccttcat ttgcagcgca tagattccac cagccacgac
     5221 cactaatagt gcgcccagca gataaaatgt gaatttaatc aggtggatac agcgatgaat
     5281 tttattcgac agggattagg catcgcctta ttaccagagt taacgctgaa aaccattgca
     5341 ggtgaattgt gttccgttcc tcacgaacca accttctatc gaca
//
LOCUS       DAZSBF010000251         5365 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZSBF010000251 DAZSBF010000000
VERSION     DAZSBF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.256"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            210..1067
                     /gene="focA"
                     /locus_tag="ACHXWD_003772"
     CDS             210..1067
                     /gene="focA"
                     /locus_tag="ACHXWD_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR7622557.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1122..3404
                     /gene="pflB"
                     /locus_tag="ACHXWD_003773"
     CDS             1122..3404
                     /gene="pflB"
                     /locus_tag="ACHXWD_003773"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR7622558.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3596..4336
                     /gene="pflA"
                     /locus_tag="ACHXWD_003774"
     CDS             3596..4336
                     /gene="pflA"
                     /locus_tag="ACHXWD_003774"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR7622559.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4468..4605)
                     /locus_tag="ACHXWD_003775"
                     /pseudo
     CDS             complement(4468..4605)
                     /locus_tag="ACHXWD_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            4705..>5365
                     /locus_tag="ACHXWD_003776"
     CDS             4705..>5365
                     /locus_tag="ACHXWD_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7622560.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1382 a         1313 c         1287 g         1383 t
ORIGIN      
        1 gttttagcgg aggatgcgga aaaaattcaa ctcatttgtt aatttttaaa atttattttt
       61 atttggataa tcaaatgttt actccgtatc tgcataaaaa ccatgcgagt tacgggccta
      121 taagccaggc gagatatgat ctatatcaat ttctcatcta taatgctttg ttagtatctc
      181 gtcgccgact taataaagag agagttagtg tgaaagctga caaccctttt gatcttttac
      241 ttcctgctgc aatggccaaa gtggccgaag aggcgggtgt ctataaagca acgaaacatc
      301 cgcttaagac tttctatctg gcgattaccg ccggtgtttt catctcaatc gcattcgtct
      361 tctatatcac agcaaccact ggcacaggca caatgccctt cggcatggca aaactggttg
      421 gcggcatttg cttctctctg gggctgattc tttgtgttgt ctgcggagcc gatctcttta
      481 cttccaccgt gttgattgtt gttgctaagg cgagtgggcg catcacctgg ggtcagttgg
      541 cgaaaaactg gctaaatgtc tattttggca acctggtcgg cgcactgctg tttgtacttt
      601 taatgtggct ttccggcgag tatatgaccg caaatggtca atggggacta aacgtcctac
      661 aaaccgccga ccacaaagtg caccatactt ttattgaggc cgtctgtctt ggtatcctgg
      721 caaacctgat ggtatgtctg gcagtatgga tgagttattc tggccgcagc ctgatggaca
      781 aagcgttcat tatggtgctg ccggtcgcga tgtttgttgc cagcggtttt gagcacagta
      841 tcgcaaacat gtttatgatc ccgatgggta ttgtaatccg cgacttcgca tccccggaat
      901 tttggaccgc agtcggttct gcaccggaaa atttttctca cctgaccgtg atgaatttca
      961 tcactgataa cctgattccg gttacgatcg gcaacattat cggtggtggt ttgttggttg
     1021 ggttgacata ctgggtcatt tacctgcgtg aaaacgacca ccattaatgg ttgtcgaagt
     1081 acgcagtaaa taaaaaatcc acttaagaag gtaggtgtta catgtccgag cttaatgaaa
     1141 agttagccac agcctgggaa ggttttacca aaggtgactg gcagaatgaa gtaaacgtcc
     1201 gtgacttcat tcagaaaaac tacactccgt acgagggtga cgagtccttc ctggctggcg
     1261 ctactgaagc gaccaccacc ctgtgggaca aagtaatgga aggcgttaaa ctggaaaacc
     1321 gcactcacgc gccagttgac tttgacaccg ctgttgcttc caccatcacc tctcacgacg
     1381 ctggctacat caacaagcag cttgagaaaa tcgttggtct gcagactgaa gctccgctga
     1441 aacgtgctct tatcccgttc ggtggtatca aaatgatcga aggttcctgc aaagcgtaca
     1501 accgcgaact ggatccgatg atcaaaaaaa tcttcactga ataccgtaaa actcacaacc
     1561 agggcgtgtt cgacgtttac actccggaca tcctgcgttg ccgtaaatct ggtgttctga
     1621 ccggtctgcc agatgcatat ggccgtggcc gtatcatcgg tgactaccgt cgcgttgcgc
     1681 tgtacggtat cgactacctg atgaaagaca aactggcaca gttcacttct ctgcaggctg
     1741 atctggaaaa cggcgtaaac ttggaacaga ctatccgtct gcgcgaagaa atcgctgaac
     1801 agcaccgcgc tctgggtcag atgaaagaaa tggctgcgaa atacggctac gacatctctg
     1861 gtccggctac caacgctcag gaagctatcc agtggactta cttcggctac ctggctgctg
     1921 ttaagtctca gaacggtgct gcaatgtcct tcggtcgtac ctccaccttc ctggatgtgt
     1981 acatcgaacg tgacctgaaa gctggcaaga tcaccgaaca agaagcgcag gaaatggttg
     2041 accacctggt catgaaactg cgtatggttc gcttcctgcg tactccggaa tacgatgaac
     2101 tgttctctgg cgacccgatc tgggcaaccg aatctatcgg tggtatgggc ctcgacggtc
     2161 gtaccctggt taccaaaaac agcttccgtt tcctgaacac cctgtacacc atgggtccgt
     2221 ctccggaacc gaacatgacc attctgtggt ctgaaaaact gccgctgaac ttcaagaaat
     2281 tcgccgctaa agtgtccatc gacacctctt ctctgcagta tgagaacgat gacctgatgc
     2341 gtccggactt caacaacgat gactacgcta ttgcttgctg cgtaagcccg atgatcgttg
     2401 gtaaacaaat gcagttcttc ggtgcgcgtg caaacctggc gaaaaccatg ctgtacgcaa
     2461 tcaacggcgg cgttgacgaa aaactgaaaa tgcaggttgg tccgaagtct gaaccgatca
     2521 aaggcgatgt cctgaactat gatgaagtga tggagcgcat ggatcacttc atggactggc
     2581 tggctaaaca gtacatcact gcactaaaca tcatccacta catgcacgac aagtacagct
     2641 acgaagcctc tctgatggcg ctgcacgacc gtgacgttat ccgcaccatg gcgtgtggta
     2701 tcgctggtct gtccgttgct gctgactccc tgtctgcaat caaatatgcg aaagttaaac
     2761 cgattcgtga cgaagacggt ctggctatcg acttcgaaat cgaaggcgaa tacccgcagt
     2821 ttggtaacaa cgatccgcgt gtagatgacc tggctgttga cctggtagaa cgtttcatga
     2881 agaaaattca gaaactgcac acctaccgtg acgctatccc gactcagtct gttctgacca
     2941 tcacttctaa cgttgtgtat ggtaagaaaa cgggtaacac cccagacggt cgtcgtgctg
     3001 gcgcgccgtt cggaccgggt gctaacccga tgcacggtcg tgaccagaaa ggtgcagtag
     3061 cctctctgac ttccgttgct aaactgccgt ttgcttacgc taaagatggt atctcctaca
     3121 ccttctctat cgttccgaac gcactgggta aagacgacga agttcgtaag accaacctgg
     3181 ctggtctgat ggatggttac ttccaccacg aagcatccat cgaaggtggt cagcacctga
     3241 acgttaacgt gatgaaccgt gaaatgctgc tcgacgcgat ggaaaacccg gaaaaatatc
     3301 cgcagctgac catccgtgta tctggctacg cagtacgttt caactcgctg actaaagaac
     3361 agcagcagga cgttattact cgtaccttca ctcaatctat gtaattagat ttgactgaaa
     3421 tcgtacagta aaaagcgtac aataaaggct ccacgaaagt ggggcctttt ttagcacgag
     3481 agcctttttt gtcagctatc tatactctaa ggtgactgcc aaaacagact cgacgtagcc
     3541 ttcgagctgc gcaccaacac ggcctcagat gggccacatc tggagaaaca ccgcaatgtc
     3601 agttattggt cgcattcact cctttgaatc ctgtggaacc gtagacggcc cgggtattcg
     3661 ctttatcacc tttttccagg gctgcctgat gcgctgcctg tattgtcata accgcgacac
     3721 ctgggatacg catggcggta aagaagttac cgttgaagat ttaatgaaag aggtggtgac
     3781 ctatcgccac tttatgaacg cttccggcgg cggcgttacc gcatccggcg gtgaggcaat
     3841 cctacaagct gagtttgttc gtgactggtt ccgcgcctgc aaaaaagaag gcattcatac
     3901 ctgtctggac accaacggtt ttgttcgtcg ttacgatccg gtgattgatg aactgctgga
     3961 agtaaccgac ctggtaatgc tcgatctcaa acagatgaac gacgagatcc accaaaatct
     4021 ggttggagtt tccaatcacc gcacgctgga gttcgccaaa tatctggcga acaaaaatgt
     4081 gaaggtgtgg atccgctacg ttgttgtccc aggctggtct gacgatgacg attcagcgca
     4141 tcgcctcggt gaatttaccc gtgatatggg caacgttgag aaaatcgagc ttctccccta
     4201 ccacgagctg ggcaaacaca aatgggtggc aatgggtgaa gagtacaaac tcgacggtgt
     4261 taaaccaccg aagaaagaga ccatggaacg cgtgaaaggc attcttgagc agtacggtca
     4321 taaggtaatg ttctaatttc cccgtaaagc ggcaacttta ttgagttgcc gcttttttat
     4381 tccgcacttc attctgctac agcgttttta acacatctta tagtgcatct accatatccc
     4441 gtacctgaga taataacgac tgataatatt acgccgatat aaatcgagtt ccgtcacctg
     4501 tattttccgc tccattgccc gctgatggat cgcatcagca atatgtgcgg tcaatgaatc
     4561 atgacgagga tgggcccata ccaaataaat acgttcagac tgcataattt tacctccacg
     4621 ttaagttctg gagcgcactt tacgcaggcg gcataataat gtaaacgtga tgattcagga
     4681 tgagttaatg aaggattttc actaatgcgg atgaatatgt ctgactttgc cactttcttt
     4741 gctgtggccc gtaatcaaag ctttcgtgca gcgggcgatg agttaggctt atcctcgtcc
     4801 gccattagcc atagtattaa aacactggaa caacgtctta aaattcgctt attcaatcgg
     4861 acaacccgaa gcgtatcgtt aacggaagcg ggttctaatc tctatgaacg attgcgccca
     4921 gctttcgacg aaattcagat catgttagat gagatgaatg attttcgtct gacaccaaca
     4981 ggaacgctca aaatcaatgc cgcccgggta gctgcacgta tatttttaat gcctttactg
     5041 gttggtttca cacgtgaata tcctgatatt aaagtcgagc tcacaactga cgactctctt
     5101 gtcgatatcg tccaacaggg ctttgacgct ggcgttcgac ttagtggcat cgtcgaaaaa
     5161 gatatgatca gcgtggcgat cggcccacca gtgaaattat gtgtagcagc aacgccagag
     5221 tactttgcaa gatatggcaa accccgccat cctcatgatc tgcttaatca tcaatgtgtt
     5281 gtatttcgtt atcccagcgg caaacctttc cactggcaat ttgcaaagga gcaggaaatt
     5341 gctgtcgcag ggaatatcat tctgg
//
LOCUS       DAZSBF010000252         5355 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZSBF010000252 DAZSBF010000000
VERSION     DAZSBF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.257"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHXWD_003777"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHXWD_003777"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR7622561.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHXWD_003778"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHXWD_003778"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR7622562.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHXWD_003779"
     CDS             complement(2698..3390)
                     /locus_tag="ACHXWD_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR7622563.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHXWD_003780"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHXWD_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR7622564.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1368 a         1385 c         1268 g         1334 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgc
//
LOCUS       DAZSBF010000253         5301 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZSBF010000253 DAZSBF010000000
VERSION     DAZSBF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.258"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(69..2852)
                     /locus_tag="ACHXWD_003781"
     CDS             complement(69..2852)
                     /locus_tag="ACHXWD_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR7622565.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAELSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2909..5281)
                     /locus_tag="ACHXWD_003782"
     CDS             complement(2909..5281)
                     /locus_tag="ACHXWD_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR7622566.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1324 a         1231 c         1209 g         1537 t
ORIGIN      
        1 aaaattatta cataaaaatg gcgaatattg ctaaaatccc cgccaacgat gtgttgacgg
       61 ggctgttatt attttggcaa taatactcca gtataagtat ttaccggatg agaaagatat
      121 tgtttaacgg cagtgttaac attctctacc gtcatttgtt tcaacaattg ctcctgctca
      181 gtccatgctg caggatcgtc atattgaata agactattta caatagtgtt cgctaattgt
      241 tgaacgctac gctgttggat atcgaggctg cgctgaacgt tttgctggta ttcattcagt
      301 tcttgctcac tgatcccttt agccagacgc ttaaccatca cttcattcgc taacgttaac
      361 agttcatcat gtcgttctgg ttgacaagta aaagccagca aatgactgat atctttggcc
      421 tgaggatcaa ccgagaggcg agaagaaacg ctgtatgctc cagatgcctg ttcacgaata
      481 ttaacgcgta gatcttttgc cagtgcgacg ttaaaagcat cgagcgccat acgcgtcgcc
      541 agattaacag gtgtccggga atcataacgc ttccactgtg aaacctgtgc cacaggttca
      601 ttttgttctt ttacagtaac cgatgcgttg tccgtcgcgc gagttaatgg tttacctgcg
      661 gctaatggcg aatcagagtg tttgattgat cctaagtaac gcgtaattaa cgccacgagt
      721 ttgtcttctg agacattacc gacaatgaca aacgtgatat ccgctggaga tgaaaacaac
      781 tggcgatcgg cagccagcgc atctgcggca gtaaactgta caatctgatt ttcttgcagt
      841 aatttcgtgc ggtcatcagc atagcgcgtc tcatacatct gctgggcgaa tttctccgct
      901 ggacgctggt cgagcgtttt caacgcctga atttgagcat tttgtagcga tgcccaaata
      961 ttgtcgttaa tcgtgctgtg ggtgattcgc tggttaatca actgaaaacc aggttcaggg
     1021 ttattagtcc gcgcgctaac ggagagcaac gtattcatgc cactgacttt actgctcatg
     1081 gttaccgaat tttccgcact ccagcgtttc aggctggaag aggagagctc accaacgccg
     1141 cttccgctta ctgctttatt tgccagcgcg ataagtgatt tttgctgcgc agggaaactt
     1201 aaatcgcctt tattagatac ggcagtaatt tgtagctttt gctcttcacc cgcggatttt
     1261 gccagaataa ccctggcacc attggaaagt gttaatgatg tcagattctc agccagcgtt
     1321 tctttgctgc taatttccgc ctgtgggtca gcgtctactg ttaacgataa atttctgcct
     1381 gggaagatgt aagccgccag ctttttgttg gcgtactctt tttccagcgc caggatagcc
     1441 gcaagagaca atgctttttt ggcggcaagc tcattgttta ccatttgctc ccaaaatgcg
     1501 tcctggttct ttcttaactg ctgccatttt tccgccagac tttgcacggt aatttgctgc
     1561 cacagacgtt tcgaaagttg atatgtctct tccggcgaca agaacggcgt attatttaat
     1621 gagctggatg ccaggcgact ggtcagcata cgtaaatcac gttcagcttg ctgatcaacc
     1681 gcatttttca gccaggtgag gcgggtagat ttgacatcat cgagttcttc agcagaaaaa
     1741 ccatgctgat caatggttgc caactctgcc attaatgcat ttgcagcatc ctgcatatta
     1801 tcgtctcgtg catttacacg gaaaaacagc gactgataat cgggtgcaat tttaacgctg
     1861 cgcgcagtgc cgccagaaat agtcttcaac tcgcccgact gtatgcgttc ctgcagacgt
     1921 tgattgaaca gctgaactaa catgctcaat tcagcttgtt cgacaaagct ttgctcatcg
     1981 ttcacttgta ccattggcag gcgataatag agtgcgatgc cgttcactcg gttttcttta
     2041 tcattaatga tattaaagcg caggtggttt tcggcttttg tcggccagac gcgattttca
     2101 gctgctttgt tagccggaag cttacttaaa ttatccttta tcagcgccag cgcttcttta
     2161 ctgtcgatat cgccgaccac gataaaggtc atattatttg gttgatacca gcgttgataa
     2221 aattggcgca attgtgccgg tgtgaccgtg gcgacagtat ccatcaggcc gataggttca
     2281 cggtctaaat tacgggtatt tgccagcagg aaagggcggc gcgcctgaga ggtgcgccat
     2341 ttcgcatcct gatgggcacg ccattcctca gtaattacgc cgcgttcagc gtctacttcg
     2401 agtatttcaa aggttgcggc attactccat tcactgaaga ttgccatcac ttgttgcaga
     2461 ttttgtttct gcgtagtcgg cagactcacc tgatacagcg tttcgtcata gctggtatag
     2521 gcattaacat cgcgaccgaa acgcaggccc attgactcaa atgtttcgat gactttatta
     2581 cccggccatg tttttgtgcc gttaaacatc atatgttcta caaaatgagc cacgccgcgc
     2641 tcattgtctt cttcctgcaa tgaaccggta tgaatttgca gccataaatt tacctgatcc
     2701 tttggttgag catgcggata aatcatatat cgcaagccat tgtccagttg cccggtaatt
     2761 aacttttcat cctgcggtaa ggcggcggca atcagccgcc cggggagcaa cagagttgcc
     2821 actaacgtca gtaagaaaca gaggtttctc ataattatct ccatgcgaaa accgggcgaa
     2881 tttacccggt taagtaaaat ccgaactatt aaaatttcat gctgacatca agccagaaag
     2941 tacggccact ggcgtaggtc gccacaccgg tgttcgttgt atcaacagcg gttttgctat
     3001 ccagtacatt gagaatatcg gcactgatcg tcaggttttg ttgtttcaga aattgtggcg
     3061 tccaggacaa gcgggtgtcc caggtcaggc tgctatccag tttttcgtca acatactgct
     3121 tgtaatctga atattcgctg atgtattgcg catttgtctt accaaggata atgcgagctt
     3181 tacgcgcttc ttgccaggcc agcgtattcg cccacactaa cccgctcggt tgatgcgtga
     3241 aatccatgtt taaggagatc tttaatgggt tattaaaatc tgccactgga acactatcgt
     3301 aagagaccag attaccgtta taaaccacct ggttatcacc ggtattgctc tcctcataac
     3361 cattgtttaa cgacaagttg cccttgctct tgatatagct aaagacaatt tgtgggttaa
     3421 tatctacttg gcggatatgc aggggttcgg ccagttcaaa actgaggctg aacgaatgcg
     3481 ttttggtttt gccatcgttg ttatattcag taatggtggt tttagtcgcg ctgtcggtac
     3541 gactgctttt gctgatttga tcatgcgctt cacggtaaac atagtttgca cgcgcaataa
     3601 cgttcttacc gattttctgc tgcaatccca ttgccagttc atcgttataa ggcgttttca
     3661 aatcctgata acgcgtcaga gttttattac ctgataccga ttccgtccag ctattgcgga
     3721 tatcacgtaa tcccatatca agaatattcc cgccatagta acggttataa ccggcggtaa
     3781 tcattgaggt ttgatcagca aaaatatccc attccgtcat aaagcgcggg gagatattgt
     3841 ggtttgacag atagttgtca tagtcataac gcacaccggg cattaacgac acattccgcc
     3901 agctaatgtg ctccgccata tacagcgtgt agttgtcaat tcccaggctg cctttacctt
     3961 tatggtaaat ggtatggtta gttttctttc cggcagcatt aatcacatag gattcagact
     4021 ggttatggcg ttcagtccat gcatcggaat agatgtattc cgcgccgaag tagggttgat
     4081 gcgaaacatc acccacggcg aatttttgcc agtccaggcg tgttttgaag gtgtaattat
     4141 ctacagcctg ggaaatgtgt cctaatccgc cacaggtaca acgccctgta atatcaccat
     4201 atgtacatga aagttcggtg taccagatgt catgatcgtg acgggtataa tcactaatat
     4261 gatcccaacc aacggtggta cgtagtttgg cccatgcgag ttgcgtatcc atatcccatg
     4321 ccagaccata ggatttatta cccatttcgc gatcagactg cgggaaggtg ctggtattat
     4381 aatcacggct ggagccggta tattttaagg ttaaatcgtg ggtaaagcgg tcgctggcaa
     4441 accaggtaaa tttgctcaat gcagtatcga taacgttttt atactgtgcc cgaccggtga
     4501 caatgccgtc attcgaaaca taatccgcgc gggtgatatc agactggcgg cgcgataaac
     4561 cggcggtaac gccgaagtta tcagcgagtt cctgattaaa cgacaaggta taaaagttct
     4621 ttttaaaatc tggggagtaa taagtacttc ctgaagagcc ttgattaaat gcgctcttgt
     4681 tattctcatc gatatgcgat gttaaccagt ccgaacgcgt agtgcgataa cccaatttca
     4741 ccttgctatc atcagcgttg aagcgtttga tctttgcatc aattaccccg ccattgaagc
     4801 gaccaaattc aaccggcaca aaactgtcat aaagcgtcac attgtccagt aagctgacat
     4861 caagataata cccctgtgac atcccgctaa tattggttgc actactggca tcggactcat
     4921 tcgctgggtt caggttatta gttgcactaa taccgtcaat caaataggca ttctggtagg
     4981 gcgacgcacc gtgaatagag attttctcag ggcgaatatc tccctggttc aacgaggtac
     5041 tttgcgttga atccatgcgt accgcagggt tggttctcag cagatcgctg atattgccat
     5101 tcccggtcgg taaacgctcg atgctttcac tggtgtaatg cgtgttgccg tttacgggta
     5161 cagggacagg agcatagacc gtcatctctt caaaaaaatg catgtacatg tttttgtcat
     5221 cgacggcctg cgccacatca gcgccacata aaatgacgcc aggaataaga actcgcttca
     5281 tttatttata tctccgtaca g
//
LOCUS       DAZSBF010000254         5296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZSBF010000254 DAZSBF010000000
VERSION     DAZSBF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.259"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..130)
                     /locus_tag="ACHXWD_003783"
     CDS             complement(<1..130)
                     /locus_tag="ACHXWD_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR7622567.1"
                     /translation="MDTRVAFMTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPS"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="ACHXWD_003784"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="ACHXWD_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHXWD_003785"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="ACHXWD_003785"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR7622568.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHXWD_003786"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="ACHXWD_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR7622569.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="ACHXWD_003787"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="ACHXWD_003787"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR7622570.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5296)
                     /locus_tag="ACHXWD_003788"
                     /pseudo
     CDS             complement(4172..5296)
                     /locus_tag="ACHXWD_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1384 a         1331 c         1261 g         1320 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagc
//
LOCUS       DAZSBF010000255         5294 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZSBF010000255 DAZSBF010000000
VERSION     DAZSBF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.260"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            165..611
                     /locus_tag="ACHXWD_003789"
     CDS             165..611
                     /locus_tag="ACHXWD_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR7622571.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            622..2151
                     /locus_tag="ACHXWD_003790"
     CDS             622..2151
                     /locus_tag="ACHXWD_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFR7622572.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVFLVCYLANDKALLGTQSGAGFLGAVVLGLAIGYFVFWFRKV
                     RLGKALQPLLGSMLIPFVTLLVFGVLTYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAA
                     FLVGAMLAFDMGGPINKTAWFFCFSLLEKHIYDWYAIVGVVALMPPVAAGLATFIAPK
                     LFTRQEKEAASSAIVVGATVATEPAIPYALAAPLPMITANTLAGGITGVLVIAFGIKR
                     LAPGLGIFDPLIGLMSPVGSFYLVLAIGLALNISFIIVLKGLWLRRKAKAAQQELVHE
                     H"
     gene            2141..3211
                     /locus_tag="ACHXWD_003791"
     CDS             2141..3211
                     /locus_tag="ACHXWD_003791"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR7622573.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3211..4959
                     /locus_tag="ACHXWD_003792"
     CDS             3211..4959
                     /locus_tag="ACHXWD_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR7622574.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(5008..>5294)
                     /locus_tag="ACHXWD_003793"
     CDS             complement(5008..>5294)
                     /locus_tag="ACHXWD_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HFR7622575.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         1218 a         1352 c         1383 g         1341 t
ORIGIN      
        1 taatgattca tgtggtttat ttaaataacc attatgtgca ttactccgca aatctgacct
       61 ttcactctgt ctcaaattgt cattgtttac cgacatatcg cctctcatca cttaatcgct
      121 ccaccattac aattgattaa aatatatttt atggagtccg attaatggca gctcttactg
      181 caagctgtat tgacctgaat attcagggca atggcgctta ttccgttctg aagcagttgg
      241 cgacaatagc gttacaaaac ggttttatca ccgactcaca ccagttcctg caaaccctgc
      301 tgctgcgcga aaaaatgcac tctactggat ttggttccgg tgtcgccgtg ccgcacggta
      361 aaagcgcctg cgttaaacaa ccgtttgtat tattcgcccg caaagcgcag gctattgact
      421 ggaaagccag cgatggcgaa gacgtcaatt gctggatctg cctcggcgtg ccgcaaagcg
      481 gcgaagagga tcaggtcaaa atcatcggca cactgtgtcg caaaattatc caccaggatt
      541 ttattcatca actgcaacag ggcgacgccg accaggtgct tgccttgtta aatcaaaccc
      601 tcagctcata aggaagtggc gatggagtca tccttatgta ttgtcgcgat caccaactgc
      661 cccgccggga tcgctcacac ctacatggtg gcggaagccc tggaacagaa agcccgttct
      721 ctcggccata ccataaaagt ggaaactcaa gggtccagtg gcgttgaaaa ccgcttatcc
      781 agcgaagaga ttgccgctgc cgattacgtc attctcgcta ccgggcgtgg cctgagcggt
      841 gatgatcgcg cgcggtttgc cgggaagaaa gtttatgaga ttgccatctc ccaggcgttg
      901 aaaaatatcg accagatttt cagcgaatta ccgacaaact cgcagctttt tgccgcagat
      961 agcggcgtga agcttggcaa acaggaagtg caaagcggca gcgtgatgag ccacctgatg
     1021 gcgggcgtct ctgcggcgct gccgtttgtc atcggcggtg gtatcctggt ggcgcttgcc
     1081 aacatgctgg tgcagttcgg tttgccgtat accgatatgt cgaaaggcgc gccgtcgttt
     1141 acctgggtag ttgaatccat cggttacctt ggcttcacct ttatgatccc catcatgggc
     1201 gcttatattg cctcgtcgat tgccgataaa ccggctttcg ctccggcgtt tctggtttgc
     1261 tatctggcga acgacaaagc gctgctcggc acccagtcgg gtgcgggatt cctcggcgcg
     1321 gtggtgtttc tggtttgcta tctggcgaac gacaaagcgc tgctcggcac ccagtcgggt
     1381 gcgggattcc tcggcgcggt ggtgctgggg ctggcgattg gctatttcgt cttctggttc
     1441 cgcaaagtgc gccttggcaa agcattacaa ccgctgctcg gttcaatgct gatcccgttc
     1501 gttactctgc tggttttcgg cgtgctcact tactacgtta tcgggccggt gatgtccgac
     1561 ctcatgggcg ggctgctcca cttcctgaat accattcctc catcaatgaa gtttgcggca
     1621 gcgtttctgg tcggtgcgat gctggcgttc gatatgggcg gccccatcaa taaaaccgcc
     1681 tggttcttct gcttctcact gctggaaaaa cacatttatg actggtacgc catcgtcggc
     1741 gttgtcgcgt tgatgccgcc cgttgcggcg ggtcttgcga cgttcattgc gcccaaactg
     1801 tttacccgac aggaaaaaga ggctgccagt agtgccattg tggtcggtgc cacagtcgca
     1861 accgaaccgg ctatacctta cgctctggca gctccgttgc cgatgattac cgccaatacg
     1921 ctggcgggtg gcattaccgg cgtgctggtg atcgctttcg gaatcaaacg tctggcacct
     1981 ggtttgggta tctttgaccc gctgatcggc ctgatgtcgc cggtgggttc gttttatctg
     2041 gtgctagcga ttggcctcgc gctgaacatt tcattcatta tcgtcctgaa aggattgtgg
     2101 ttacgccgca aagcgaaagc cgcgcaacag gagcttgtcc atgaacattg agttactgca
     2161 acagttgtgc gaagccagcg ccgtcagcgg cgatgaacag gaagttcgcg acattctgat
     2221 aaacacgctg gaaccttgcg ttaatgagat cacctttgat ggtctgggca gctttgttgc
     2281 ccgtaagggg aataaaggtc caaaagttgc cgttgtcagg catatggatg aagtcggctt
     2341 tatggtcacc cacatcgacg agagcggttt tctgcgcttt accaccattg gcggctggtg
     2401 gaatcagtca atgctcaacc accgggtaac catacgcaca cacaagggat ttaaaatccc
     2461 tggtgtgatt ggttccgtcg cacctcatgc gttaacggaa aagcaaaagc aacagccgct
     2521 gtcatttgat gagatgttca ttgatattgg cgcgaacagt cgcgatgagg tggaaaaacg
     2581 cggtgtggcg atgggcgatt ttattagccc ggaagccaat tttgcctgct ggggcgaaga
     2641 taaagtggtc ggcaaggcgc tggataaccg catcggctgc gcaatgatgg ctgaactatt
     2701 gcagacggtg aataatcccg aaattacgct gtatggcgtc ggcagcgtgg aagaagaagt
     2761 tgggctacgc ggggcacaaa cctcggcgga acacattaaa ccggatgtgg tgattgtgct
     2821 ggataccgct gtcgcaggtg atgttccggg cattgataac attaaatacc cgctgaaact
     2881 gggccagggg ccggggctga tgctgtttga caagcgctac ttccccaacc agaaactggt
     2941 ggcggcgtta aaaagctgtg ccgcacataa cgatttaccg ctgcaatttt ccaccatgaa
     3001 aaccggtgcg acggatggcg ggcgctacaa cgtgatgggc ggcgggcgtc cggttgttgc
     3061 gctgtgcctg ccaacccgct atctgcacgc taacagcggc atgatttcaa aagccgatta
     3121 cgatgctctg ctcacgttga tacgggattt tctgaccacc ttaaccgcgg agaaagtcaa
     3181 cgcgtttagc cagttccgtc aggtggatta atgctaaacg aacgccagtt aaagattgtc
     3241 gatctgctgg agcaacagcc gcgcacgcct ggtgagctgg cgcaacagac tggcgtttca
     3301 ggcaggacca tcctgcgtga tattgactat ctcaacttta cccttaacgg caaagcccgc
     3361 attttcgcca gtggcagtgc gggctatcag ctggaaatct tcgagcgccg cagctttttt
     3421 cagttgctgc aaaagcacga taacgacgat cggctgctgg cgctgttatt actgaatacc
     3481 tttactcccc gtgcgcaact cgcctcggcg cttaatttgc cagaaacctg ggtagcagag
     3541 cgtctgcccc ggttaaaaca gcgttatgaa cgcacttgtt gcctggccag tcgccccggt
     3601 ttgggccatt tcattgatga gacagaagag aaacgcgtta tcttgctggc gaacttgctg
     3661 cgcaaagatc cgtttttaat tccgctggcg ggcgtaacac gagacaattt tcagcattta
     3721 tccacggcct gcgacaacca acaccgctgg ccgctcatgc agggtgatta tctctccagc
     3781 ctgattctgg cgatttacgc cctgcgtaat caactgaccg atgagtggcc gcaatatccc
     3841 ggtaacgaga taaaacaaat cgttgaacag agcggcatgt ttcttggtga taacgctgta
     3901 agaaccctga caggtttgat agagaaacag catcagcaag cgcaggtaat ttcagccgat
     3961 catgtgctgg ggttgctgca aagggtgcca ggcatcgcgt cattgaatat tattgatacg
     4021 cagctggttg agaatattac cgggcattta ttacgttgcc ttgccgcacc ggtgtggatt
     4081 gccgagcacc gccaaagcag catgaataac ctgaaagccg cctggcctgc ggcctttgat
     4141 atgagtctgc actttattac gctactgcgt gaacagctcg atattcccct tttcgatagc
     4201 gatctgatcg gtttgtattt tgcctgtgcg ctggaacggc atcaaaacga acgccagccg
     4261 atcattttgc tctcggacca gaacgcgatt gccactatta atcagctcgc cattgagcgc
     4321 gatgttttac attgtcgggt gattattgcc cgtagcttaa gcgagcttat tgccattcgc
     4381 gaagagattg agccgttatt gatcattaac aacagccatt atttactgga tgacgcggta
     4441 aataattaca tcaccgtaaa aaatatcatt acggctgccg atatcgaaca aataaaacac
     4501 tttctggcga cggcatttat tcgccaacag ccggaacgct ttttctctgc ccccggaagt
     4561 tttcattatt cgaatgtacg cggtgaaagc tggcaacata ttacccggca aatttgtgcg
     4621 caattagtgg cacaacacca tattaccgcc gatgaagcgc aacggatcat cgcccgcgaa
     4681 ggcgaaggtg aaaacctgat tgttaatcgc ctcgccatcc cacattgctg gagcgaacag
     4741 gagcgacgtt ttcgtggatt ttttattacc ctcgcccaac cagttgaggt gaataacgaa
     4801 gtcattaacc atgtcttgat cgcctgcgcc gccgccgatg cgcgtcatga gctaaaaata
     4861 tttagctatc tggcaagcgt attgtgtcag catccggcag aagttattgc cgggttaaca
     4921 ggatatgagg catttatgga gttacttcac aaggggtgaa ctgtgtcgga tgcgccgcat
     4981 ccgacactgc attttcatca aaaacgttta acgcaggcta ttatagtcat caaccatttc
     5041 gttgtactcg cgtaacgctc gtggaaatga gtcttcgacc atccagcttg tgtttgcatc
     5101 ctgaagatgc tctttatccc agtcagagta cgggacttta tctctgacgc ggcgaacata
     5161 ttttttagtc tcgagttgat actggtctgc cgaattaatg aacgagaaat tcataccgcc
     5221 tttatccgct tctttggcct gcgctaccag cgtttccagt tcagagactt tcttcattgc
     5281 cgcatcaaca tcaa
//
LOCUS       DAZSBF010000256         5271 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZSBF010000256 DAZSBF010000000
VERSION     DAZSBF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.261"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..471)
                     /locus_tag="ACHXWD_003794"
     CDS             complement(<1..471)
                     /locus_tag="ACHXWD_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7622576.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="ACHXWD_003795"
     CDS             complement(471..1391)
                     /locus_tag="ACHXWD_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7622577.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHXWD_003796"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="ACHXWD_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR7622578.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="ACHXWD_003797"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="ACHXWD_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR7622579.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTARFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5271)
                     /gene="ynaI"
                     /locus_tag="ACHXWD_003798"
     CDS             complement(4429..>5271)
                     /gene="ynaI"
                     /locus_tag="ACHXWD_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR7622580.1"
                     /translation="VIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQQIK
                     KGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILS
                     NFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSS
                     ISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQF
                     ADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPP
                     EQGR"
BASE COUNT         1424 a         1203 c         1225 g         1419 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaacggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccagattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa c
//
LOCUS       DAZSBF010000257         5234 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZSBF010000257 DAZSBF010000000
VERSION     DAZSBF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.262"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHXWD_003799"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHXWD_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHXWD_003800"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHXWD_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR7622581.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHXWD_003801"
     CDS             complement(1327..3060)
                     /locus_tag="ACHXWD_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR7622582.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHXWD_003802"
     CDS             complement(3057..3551)
                     /locus_tag="ACHXWD_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR7622583.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHXWD_003803"
     CDS             complement(3673..4023)
                     /locus_tag="ACHXWD_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR7622584.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHXWD_003804"
     CDS             complement(4066..4530)
                     /locus_tag="ACHXWD_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR7622585.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHXWD_003805"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="ACHXWD_003805"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR7622586.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5230)
                     /locus_tag="ACHXWD_003806"
     CDS             complement(5024..5230)
                     /locus_tag="ACHXWD_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR7622587.1"
                     /translation="MEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGSLLL
                     GLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1145 a         1530 c         1299 g         1260 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctga
//
LOCUS       DAZSBF010000258         5219 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZSBF010000258 DAZSBF010000000
VERSION     DAZSBF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.263"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..227
                     /locus_tag="ACHXWD_003807"
     CDS             <1..227
                     /locus_tag="ACHXWD_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622588.1"
                     /translation="KNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPA
                     IIHSALNPTHYYQPVNDETTVMEIIKAYSK"
     gene            complement(291..575)
                     /locus_tag="ACHXWD_003808"
     CDS             complement(291..575)
                     /locus_tag="ACHXWD_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="HFR7622589.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="ACHXWD_003809"
     CDS             complement(593..790)
                     /locus_tag="ACHXWD_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622590.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="ACHXWD_003810"
     CDS             1002..1298
                     /locus_tag="ACHXWD_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622591.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="ACHXWD_003811"
     CDS             complement(1843..2076)
                     /locus_tag="ACHXWD_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFR7622592.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="ACHXWD_003812"
     CDS             complement(2083..2613)
                     /locus_tag="ACHXWD_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFR7622593.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2651..3349)
                     /locus_tag="ACHXWD_003813"
                     /pseudo
     CDS             complement(2651..3349)
                     /locus_tag="ACHXWD_003813"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="ACHXWD_003814"
     CDS             complement(3291..3614)
                     /locus_tag="ACHXWD_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFR7622594.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            3679..3870
                     /locus_tag="ACHXWD_003815"
     CDS             3679..3870
                     /locus_tag="ACHXWD_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR7622595.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4538..4651
                     /locus_tag="ACHXWD_003816"
     CDS             4538..4651
                     /locus_tag="ACHXWD_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622596.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
BASE COUNT         1373 a         1427 c         1116 g         1303 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaacactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaggag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaatt cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagagagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggtggcagc tccttctccg
     3121 cgaacgacag cacaccggac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 gtacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccac cttttccccg ggcatgaaac ttaactatca
     3301 tcccgcgtcc cctgttccct gacggccaga cgcagccggc gcagctcatc cccgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgctgtct gattcaggtt attcccgatg gcggccagct gacgcagtaa cggcggtgcc
     3481 agtgtcggca gcctcccgga acgggcaacc ggctcaccca ggcagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcgcgtcagc aggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtacgcca tgccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 cagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAZSBF010000259         5165 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZSBF010000259 DAZSBF010000000
VERSION     DAZSBF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.264"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHXWD_003817"
     CDS             complement(<1..229)
                     /locus_tag="ACHXWD_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR7622597.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYN"
     gene            complement(226..1896)
                     /gene="menD"
                     /locus_tag="ACHXWD_003818"
     CDS             complement(226..1896)
                     /gene="menD"
                     /locus_tag="ACHXWD_003818"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR7622598.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(1985..3280)
                     /gene="menF"
                     /locus_tag="ACHXWD_003819"
     CDS             complement(1985..3280)
                     /gene="menF"
                     /locus_tag="ACHXWD_003819"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR7622599.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3359..3664)
                     /gene="elaB"
                     /locus_tag="ACHXWD_003820"
     CDS             complement(3359..3664)
                     /gene="elaB"
                     /locus_tag="ACHXWD_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR7622600.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3719..4162)
                     /locus_tag="ACHXWD_003821"
     CDS             complement(3719..4162)
                     /locus_tag="ACHXWD_003821"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7622601.1"
                     /translation="MHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDLTGDNRH
                     ILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDTCTHHWP
                     DKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4245..5162
                     /gene="rbn"
                     /locus_tag="ACHXWD_003822"
     CDS             4245..5162
                     /gene="rbn"
                     /locus_tag="ACHXWD_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR7622602.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         1199 a         1439 c         1315 g         1212 t
ORIGIN      
        1 tgttgtaact aaccagggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
       61 taatcgccgc cgaaccaccg tgacctggga gataaacata caaccgtgag tagtcggcaa
      121 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
      181 gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg caggatcata aatggcttac
      241 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
      301 taccgtggtg gttggcgtgc gccaggcatc ggcaagtgcc gtttcaagtt cctgccagtt
      361 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
      421 cggcatcaga tagaaacgct cgcgctcgct tttcggcgtt ggcagcagcg agaaaatttg
      481 cccgccgttg ttgttcacca caattaatat cagcggcgcg gaaacctgac gcaataacgc
      541 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
      601 gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg tcgataccgc tggccccacg
      661 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
      721 caggctgtta ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc
      781 cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc
      841 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
      901 gtcggcaata ttggcaatta agcggcgtcc acgatggtgc gccggatcaa gccgcccttc
      961 gatgtcatcc acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg
     1021 tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgttgca gttcgctggt
     1081 cgctttggca ttgcctaacc agagatcggc acacggcagc ggctgtccgg tttgcgacag
     1141 cacgtcgcca atcagcggcc agccaagagt ttgcgcccac agtgcaactt ttttgccctc
     1201 ttccgcactc atgcgcccgg caaccaccac gccgcgtttt tgtcgccaga agaaccagtc
     1261 gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
     1321 ccagtcgccc agccgctgtt gccagctaag cccggtgtcg tccatttcgc catacagcgg
     1381 ttcggcaaac gggcagttga tatggactcc accagaatga agcgtaccga gagcatggtc
     1441 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
     1501 gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc gcctgattcg cgccgcagtc
     1561 aatcagctcc ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc
     1621 ttcaatcagt gccggataga gatttgctac cgccgtaccg gaggtcacaa tcaccgccac
     1681 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
     1741 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
     1801 cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
     1861 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
     1921 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
     1981 attactattc catctgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
     2041 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
     2101 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgctgt aatgagagat
     2161 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
     2221 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
     2281 tcacattatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
     2341 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaaccgtt
     2401 ggcagatatc ttccaccacc agcatattct cgcgctggtt tttatcatcc gccatcagcc
     2461 actcacctaa ctgctgcgcc tgcttatcat caggatgatt tgctaccgtt cccgccagcg
     2521 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
     2581 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
     2641 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
     2701 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
     2761 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
     2821 gcaagggctt gatactcacc agtgtggcga taaattctct tgccttaatc gcatcgtgct
     2881 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
     2941 attccaggcg tggtaaaagt aaattgccct gcgacgggtc aaacgcattc agcccccaaa
     3001 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
     3061 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
     3121 agaattgcgg gtatgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
     3181 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
     3241 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
     3301 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
     3361 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
     3421 gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt
     3481 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
     3541 gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt
     3601 ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
     3661 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
     3721 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
     3781 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
     3841 ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt
     3901 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg
     3961 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
     4021 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
     4081 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
     4141 ccgataattc agagtgatgc agatcttacc attcaatcat aatgctctcc ttttagatgt
     4201 atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
     4261 taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
     4321 tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc
     4381 agctactgca taccgccttt aaccctggga aactggacaa gatttttatc agtcaccttc
     4441 atggcgatca tctctttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
     4501 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
     4561 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
     4621 ttctcgatga tggcctgcgc aaagtgaccg cttatccgat ggaacaccca ctggaatgtt
     4681 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
     4741 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
     4801 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
     4861 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
     4921 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
     4981 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggcaagc
     5041 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcaacac ctgttacgtg
     5101 aatgcaggtc aattttcccg gcgactgaac tggcgaatga tttcaccgtg tttaacgttt
     5161 aactc
//
LOCUS       DAZSBF010000260         5128 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZSBF010000260 DAZSBF010000000
VERSION     DAZSBF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.265"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1094)
                     /locus_tag="ACHXWD_003823"
     CDS             complement(<1..1094)
                     /locus_tag="ACHXWD_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR7622603.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
     gene            complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHXWD_003824"
     CDS             complement(1131..2576)
                     /gene="abgB"
                     /locus_tag="ACHXWD_003824"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR7622604.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHXWD_003825"
     CDS             complement(2576..3886)
                     /gene="abgA"
                     /locus_tag="ACHXWD_003825"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR7622605.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4062..4970
                     /locus_tag="ACHXWD_003826"
     CDS             4062..4970
                     /locus_tag="ACHXWD_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7622606.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
BASE COUNT         1272 a         1330 c         1298 g         1228 t
ORIGIN      
        1 atgttgctcc agttaaacat ggcgacaaac tgggcgaggg gaaaaaccat cacgataaat
       61 cctgccatct ctttcatcgg ttcaatcatt aactgcggta aatccgcctg acgtcgaatt
      121 gtgcgggtag cgatgccata agccagcgag acaacaaaga aaaaaagaat gatcagtggc
      181 acgatacctt taataaaggg tgatggcatc acggtgtgat taatcggatc gcgcaatatc
      241 ccgttttccg ggatcaccat cagcgcaatc gcagcaataa aaagtagcga tacgacacct
      301 gctatgcgta aaccaaaacg ctgactttcg gtcaatgtct gcagtttctc atcgctgttt
      361 ccctgccatt gacctaaccg tggctcgatg attttgtcgg ttatcaggcc gccaacaatc
      421 gtcagtacga ctacggagct ggccataaaa taccagttat caattacact gacgtgcatt
      481 tgcggattga acgcagccgc cgcttccgtg ctgatccccg acagcaacac gtcggttgtg
      541 acaatcagta aattagccgt aaagccgcaa cctacgcctg caatggcagc cagtaaacct
      601 gcaactggat gcctgcccac cgccagaaaa atcagcgcac ccatcggtag catgatcact
      661 aacgtcgcat cggaagaaat gtggctgaaa aaagcaataa acagcaccat ataactggcg
      721 tagcgggcat taacatgcga tgccatttta accattagcg ctggcagtaa gccgacgcgc
      781 tccgccagac cggcacctaa aaccagcgcc aggatcgcac caagtggagc aaaaccgcta
      841 aagtttttaa taacattggg taaagaccag tgtaatcctt ccacactgag caggtttttc
      901 accaccaccg gcgttccatc ggtcgggttt ttcgcactga cgccaaaggc cgacaaaatt
      961 gccgtcgtca ccatgagtac gataatcaaa tagataaaga gcaaaaaagg atggagaacc
     1021 ttgttaccaa ttctttcgac ccagccatag agcttcccgg attgggagga cgacggtatg
     1081 gatgacatac tcatgggcat tcctcggttg ttgtgtttgc gttgttgttg ttattttaaa
     1141 ggtgacggtg tcacgttttt cgggataggg cagtgatacg gttgcgtgtc cgttacttgc
     1201 tgatgctctt gttggcattc ttgcaatagc cctgaatcaa tgaagagatt gactgtggtt
     1261 gctgccatag ttttcgccgc cagcagcatt cctttatgag caatagatgt tcgcccctgg
     1321 ctaaccagtt gccacgtatg tagcggtgta ccgacggcaa aacaggggct gaaacactgg
     1381 gcaacaggca gtttccaact aacgtcgccg acatcagtcg atgccgcaag cacgttatcg
     1441 gtggcggcat atggagcgac ctcattcgcc agtaccgttt cacgatgacg tagtgcaaaa
     1501 gccttgccgt tttcgccacc ggttgcggcg atattattca gactgttttg ccgatcgttg
     1561 ggggtgagcg tagcctgaat ttgtttcgca aaagccagtt cttcggagtt ccattccggg
     1621 gtaccaaaat gggatagggc atggtacatg gcattttcta aggtgcgatt cgggagataa
     1681 ctggaacagg ctttgtcgaa gcggcattca accgtggttt cggtcatcaa tgccgcacct
     1741 tcggcgattt tggcgacccg atcataaata tgctgcacgt cggtcatttc gggggcgcgg
     1801 ataagataaa gcacttctgc ctgcgcctgg accacgttgg gcgagatccc gccgctattt
     1861 gtgatggcat agtgtacgcg cgctttttca ataatatgtt cgttgaggaa gttggtgcca
     1921 gtggtcatca acgttacggc atcaagggcg ctgcgtccca aatgagggga attcgcggca
     1981 tgtgctgcga tccctttaaa gcgccatgat gcctgaatgt ttgccagcgt gcgggtattg
     2041 aacataccgg caaaggcttc cgggtgccag gtgagtgccg catccacatc atcaaatacc
     2101 ccctcgcgga ccatgaacgt tttacccgag ccgccttctt cgccaggaca accataaaag
     2161 cgcactgtgc cgccttgccc atattgttcc agccatttct tgacggctat tgcagcggca
     2221 aaggcggcgg ttccccgcaa attgtgtccg caaccgtgac cattttcacc gggcgtcgcg
     2281 gatgtaggtt gcgcgcaacc agcttgctga cttaaacctg ccagggcgtc atattctccc
     2341 agcagggcga taaccggttt gccttgacca aacgaagcaa taaaggcatt tgggatattg
     2401 cctacgttgc gggtaacggt gaagcctgca gattccagag ccgaagccag atgctccgct
     2461 gaccagaact cttcaaaacg tgtttctgga tgatcccaga tttgatcggc aatatcggta
     2521 taacgttggc gatcggcttc aatcgcatcg tcgataaaac gatagatttc ctgcatcaga
     2581 tacctcgcgt ccagggaaaa ttgagcgcgg tgcgcgccag cgtttcgacg gcaatagcga
     2641 gaacctgctc gtcaaaatcg aatttttcgt tgtgatgacc tgccgccagc tgtgtgccaa
     2701 acaccacgta ggaggcttgc ccttgatgtt gctgcacgcg ggccatcatt aatgtggcat
     2761 cttcggaacc cgcaggcgct tcaacacgtt caatggcctg attgaccccc gcgacctgag
     2821 ccgcctgact ttgcaaccat gcgacccatt gcggcgaagg agaactggcg gtaactgcac
     2881 ccatcagacg agtttcaacg ccgacaccat acatggttgc tgcgccctga atcgcttgtt
     2941 gtgcacggtc aaaaacatat tgattaatga cgtcgctggc cccgcgtgtt tccactttca
     3001 gcaacgccga ggcaggaaca acgttacgac cgcttcctgc ctgcataacg cccacgttta
     3061 ctctggaagc tccttcgctg tgcggggcga ttgcatgcag tgcaagagtg gcttgtgctg
     3121 ccgccaacaa ggcattgtga ccgtcttctg gttttgcgcc tgcgtgagcg gcggtaccgg
     3181 tgaagtgcgc gtcaaatttg gtggttgcca taaaattatc actgccgcac accacggtgc
     3241 ccgcaggtac gccagtgcca atgtgcacgg cagtaaaata atcaacatca tctacgacac
     3301 ctgcatcgac catcgcccgc gcgccacgcg taccttcctc tgcaggctga aaaatcagtt
     3361 tgatgacgcc atgtagtccg gactcgaatt gtttaagggt atgcgccagc ccaagcccaa
     3421 tggcggtatg tccatcatga ccacaggcat gcatcattcc ggcgttacat gacgcaaaac
     3481 cgtcgcggta ggggcgatgg ctgacatcct gctcttcact gagatccagc gcgtccatat
     3541 cgacacggaa agccatcacc ggaccggggc gaccggtatc cagggtggcg acgataccag
     3601 tgaaaccacc ttcaaaaacc gcaatccatt gtgctagcgc accctgttga cgagcgcgct
     3661 cgaattcgcg ttgtagagtg agttcatcag gtagtcccat ccggctactt tcattaacta
     3721 cttcgcggcc cagcgccagt gaatagccga gctgctgcaa ttcttccgca acaagggtgg
     3781 cagtgcggaa ttccacccag ccagactctg catagtgatg aaaatcacgt cgccagtgcg
     3841 ataattttgg ggcaagcgaa ttaacaaatt gattcaaaga ctccataacc tttcccgtca
     3901 tcagtaaaaa gtgtgacccg gttcacgtag cgatagtttt tacttatcac taactgattt
     3961 ttcacagttt taaccgttca taaattaccc tgacacaatc atctgcatta aagtagatgc
     4021 cagtttcttt ggtctgataa ataacggtta tcggtggcgt catggctttt caggtaaaaa
     4081 ttcatcaaat tcgggctttt gttgaagtgg ctcgtcaggg cagcattcgc ggagcgagcc
     4141 gaatgttgaa tatgtcgcaa ccggcactga gtaaatctat tcaggagctg gaagaaggat
     4201 tagcggcgca actctttttt cgccgtagta aaggcgtgac gttaactgat gccggtgaaa
     4261 gtttttatca gcacgccagt cttattcttg aagagctgcg cgcagcccaa gaggatattc
     4321 gccaacgaca agggcaactg gcagggcaga ttaatatcgg catgggggcc agtatttccc
     4381 gcagtctgat gccagctgtc atatctcgtt ttcatcagca gcatccgcag gtaaaagtac
     4441 gcattatgga agggcaactg gtgtcgatga ttaatgaatt gcgtcaggga gaattggatt
     4501 tcaccatcaa tacctattat cagggaccgt acgaccacga atttactttt gagaaattac
     4561 tggaaaagca attcgcgatc ttttgccgcc cgggacaccc cgccattggt gcccgttcga
     4621 tcaaacagtt actggattac agctggacaa tgccgacgcc acacggcagt tactacaaac
     4681 agttgagtga attgcttgac gatcaggcgc aaacgccaca ggtcggtgta gtctgcgaga
     4741 cgttctcagc ctgtatcagt ctggtggcaa aaagcgattt tctcagcata ctgcctgaag
     4801 aaatgggctg cgatcccttg cacggacagg ggctggtgat gttgccggtt agcgaaattt
     4861 taccgaaagc ggcctattat ttgattcagc ggcgtgatag tcgccagaca ccactgaccg
     4921 cgtcattaat cacgcaattc cggcgagaat gcggctatct gcaaagttaa aactgcataa
     4981 aaaaatagag tctgtcgaca tccgccagac tctacagtac acacagcagt gcatccgcgt
     5041 cttaaatccg gcattgtctc ctctgcgccg gtgactgtgt tataacggtt ttaaccttta
     5101 gttgccaatt ttcttcagtg acagattt
//
LOCUS       DAZSBF010000261         5114 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZSBF010000261 DAZSBF010000000
VERSION     DAZSBF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.266"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..379
                     /locus_tag="ACHXWD_003827"
     CDS             <1..379
                     /locus_tag="ACHXWD_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622607.1"
                     /translation="KKTNIYFDDNFLNIYKRSQDRAPQIISDLKKHNIEIGEKLNDCR
                     KKLNISDVKLTYYWFVTDTGGVDYLDGVLTYNFQGKNRAENFHKQLLQYAYINYSKNQ
                     IPRDFVTSIKNSIYYKIEDCTKF"
     gene            complement(575..2125)
                     /locus_tag="ACHXWD_003828"
     CDS             complement(575..2125)
                     /locus_tag="ACHXWD_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001624483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFR7622608.1"
                     /translation="MASYHCTVKAGAKGSALKHADYISRSGEYKSYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ASIPQTAGERKAALVALRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQQEQEQKQKIKAVEPVRSARSPEL
                     LLQYRKVMKTVIQGEARLARLGDANPNALKEHKLLQNAKAKKDSLSEWSRRIYEGARY
                     LDKLGRNVVSAQRELRELQEQRNALNGIRGLFRGADKREIDARILEQKSVLETAEHER
                     NEFRNKLQQAESEWDKENAAFKRTEGYKYVGELDRYREREILAAASLENTRQKVAEEV
                     SVARSQMLSLEPELSISGDEKAQMRHELLAEMAQERQQQEEKALCIQRSWARGVSRSN
                     ERDQDMER"
     gene            2297..2743
                     /locus_tag="ACHXWD_003829"
     CDS             2297..2743
                     /locus_tag="ACHXWD_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFR7622609.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPHNHPKWQEWEKRGKELLTKDSA"
     gene            complement(2937..3872)
                     /locus_tag="ACHXWD_003830"
     CDS             complement(2937..3872)
                     /locus_tag="ACHXWD_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622610.1"
                     /translation="MSAALQYFEENLPHRPYHTDELAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDRVGAAIDWSDRNAPAPNITVKNPENGHAHLLYALNLAVRTAPDASV
                     KALKYAAAIERALCEKLDADVNYSGLICKNPFHLEWQVMEWREEAYALDELADYLDLR
                     ASERRSVDKHYGLGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGKSIAKYTHRNFTPESFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDK
                     RFMALCMLENGYSQKAIAAMLNVHRNTIRNWAMHK"
     gene            4384..>5114
                     /locus_tag="ACHXWD_003831"
     CDS             4384..>5114
                     /locus_tag="ACHXWD_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622611.1"
                     /translation="MGKMNINFKHILPLLSSFFISGCDSDGISKVKSFVYYDIDDSMT
                     VGKAFDTRSDCINGTWSEKEDDRGRTIVTYTCDISESGLKIINSAIMQEPEDKAEYSI
                     AQNNNSIKSTNESLEEYKQKTPLVEKTKEVIIDHIRKLNSAFNEDPMIYSKLTISPYL
                     DRMLYLKDHNDNALNEICGGYEYIQSADSYSWRDVSEEYAKESCEKHIYKVYQSFKKN
                     ASPLITKNFPGFYERVPPCENADEC"
BASE COUNT         1417 a         1244 c         1127 g         1326 t
ORIGIN      
        1 aaaaaaaaca aatatatact ttgatgataa ctttctaaat atctacaaaa gaagccaaga
       61 tagagcacct caaataattt ctgatttaaa aaaacacaat atagaaatag gtgaaaagtt
      121 aaatgattgt aggaaaaaac taaatatctc agatgtcaaa ttaacctatt actggtttgt
      181 aacagatacc ggaggagttg attatttaga tggggtgttg acatataatt ttcagggaaa
      241 gaatagagct gaaaactttc ataaacaact attgcaatat gcatacatta actacagcaa
      301 aaatcaaata cctcgggact ttgtaacatc aataaaaaat agtatttatt ataaaataga
      361 agattgcact aagttttagt ccttccctta caggtggtta cacatataac cacctgcaaa
      421 tatttaacac ctgttaactt cattaatctg aactgtgagt aaatcaaaac gacaattttt
      481 ctcattcaga aaacgtttca cgggacgcgg aattttttcg cgttccgtga aacgttggcg
      541 gggttcgggg cgcagtcccg atattgctac attttcaacg ctccatatct tgatcacgtt
      601 cgttactacg tgataccccc ctggcccagg atcgctgtat acacagtgct ttttcctcct
      661 gttgctgccg ttcctgcgcc atttctgcca gtaactcatg tcgcatttga gctttttcat
      721 ctccagatat actgagttca ggctccagag ataacatctg gctacgggca acggatacct
      781 cttcagcaac cttttgcctg gtgttctcta aagatgccgc cgccagaatc tcgcgttctc
      841 tgtacctgtc cagctcccca acgtacttat atccctcggt acgcttaaaa gcagcgtttt
      901 ctttgtccca ctcggattcg gcttgctgta gcttgttcct gaattcattc ctttcatgtt
      961 cagcggtttc cagcacactt ttttgctcca gaatacgcgc atcaatctcc cttttgtcag
     1021 ccccccgaaa caggccacga atcccattca aagcattgcg ttgttcctgt agctcccgca
     1081 gctccctttg tgcggaaaca acattacgcc ccagtttgtc taaataccgt gccccttcat
     1141 atattcgtct actccattcg gaaagagagt cttttttagc tttcgcattc tgaagcagct
     1201 tatgttcttt gagcgcattg ggattggcat cccctaaacg agcaagcctt gcctctccct
     1261 gaatgaccgt tttcataacc ttgcggtact gaagcaataa ctccggtgaa cgggctgaac
     1321 gaacaggctc aactgccttg attttttgct tttgctcctg ttcctgctga atggcatttg
     1381 cggcatctct ggcggtcttg agttcccgct cggcattcct ccgttcctgg attgcaacaa
     1441 tctgctcccc attcagactg gcggcctgaa cggggccaag atgaacttcc ggcgtgcgat
     1501 tgataccctg ttctttcaga gaacgatgat ctacgcgcga ctcgtgaccg tgtcgcgcaa
     1561 ggtgctcatt ctgcacatct gcccagcggg aacgcagcgc aacaagggcg gcttttcgct
     1621 ctccggctgt ctggggaata ctcgcttttt ttgcgcctcc ccgctctggt gatttgctgt
     1681 tggcacgttt aaaaaattgc tgtggatcgc gttcaatacc atcattaatc cgctcgcaaa
     1741 acatgatatg cgcgtgtggc tgttcctttc cgtcgattgc acctggcgga ttgtggatcg
     1801 cgtactggta ggcatggcga tcgccaagtt ccttttgcac gaaatcttca acaagttcta
     1861 tccgttgttc gcgtgtcagt tcacgcggta acgcgatctc aatttcacgg tatgcagtcc
     1921 cgttttttcg ttcaaattca tctgcggctt tccatagctc tgcgggattt ttcttcgccc
     1981 atgatggcat gttaccggat gacgaaaatt ccagatcctc ccgcgatttg taactcttat
     2041 attcgccgga tctggaaatg taatcagcat gttttaaggc actgcctttt gctccagctt
     2101 tgacagtgca atggtagctt gccatctgcc ttttagccct ccgcaggagc gcacagtttc
     2161 tcgacgaagg agagaaacta ctaagtgcgc atccctgaat taatcatgga tgattttacg
     2221 gtctcacacg caggcaaaca aggtgatatt atgcataata acaactcaca ttaacggcaa
     2281 aagtgcagag gtaagaatga caaatcgtga agagtggtta agtgcaaaaa ttgcctacat
     2341 caacggactg aaatcaccat cagagcagca gagattactt gtgttactgg cagagaaaaa
     2401 aaaccgaaca acgacggatg aaaaaacgtt gtccgcactg atacgggcag aaaaaaccgc
     2461 agagaaagca gccgcagcaa aagccagagt aacggcaatc atagcggcag aacggaaggc
     2521 agccgcaagg gcggaacgaa aggcgcggga tcatgaactc tacaaggccg caggcctcat
     2581 gattgtggca ggactggtgg acagcaaaac cggaaagcca aagtttagtg ctgccgaact
     2641 ggtaggcgca ctcgcaggaa tcgcagagct accgcacaac catccaaaat ggcaggaatg
     2701 ggaaaaacgc gggaaagagc tactcaccaa agacagtgcg taagagcctc cgttcacgtt
     2761 ccagttccct ggtgcttcct cgctcattcg ctcgctacgc tcggtcatga gactgcggcg
     2821 agcacgacca cctcaatcat acggtaagcc gcaccattgc tgggctgaaa ggagaaaacc
     2881 gccggaaagc ccgctgctca aaaagacgcc gaaaaggcgc tgttatctga tataggctat
     2941 ttgtgcattg cccaattacg gatcgtatta cgatgtacat tcagcatcgc cgcaatagct
     3001 ttctgagaat atccattctc cagcatacat agcgccatga aacgcttgtc atcgtaggct
     3061 tcgcctttag cgatgccacc tttccggcct ctcttggcct gtatttctgg tgtgtgcgta
     3121 tcagccacat actgtgcgaa actttccggc gtgaagttcc tgtgcgtgta tttcgcaatg
     3181 ctcttgccaa tagcccgaca ttcaggcgga gaaagcggaa cgggaagcga tgcgttgtac
     3241 atttcgacac gctgaatcac agcatcaagc cactgtgaga atgctggcca gccctgccga
     3301 atcgccctgt aagcccattt gcgcgtcatt tcgaacagat ggcagtttcg ccccagcccg
     3361 taatgtttat cgacgctacg acgctctgag gctctcaaat cgagataatc agccagttca
     3421 tcgagggcgt aggcttcctc gcgccactcc atcacctgcc attccaggtg gaacggattt
     3481 ttgcaaatca gtccgctgta gttcacatct gcgtcaagtt tctcacacag cgcacgttca
     3541 attgcagcgg cgtatttcag cgccttaacc gacgcatcag gcgcggttct cacggcaaga
     3601 ttgagcgcgt agagcaggtg agcgtgcccg ttctcgggat tttttacggt gatgttcggc
     3661 gcgggggcgt tccggtcgct ccagtcaatc gcagctccta cgcggtcaac gtcaaaaacc
     3721 agccagaact gcgcatgagg ctgattttgc tggatgtagc gagcaagcag cgcacgccct
     3781 ttgccggaga tacgaagacc gaaagcgagc tcatccgtgt gatagggacg gtgaggtaaa
     3841 ttttcttcga agtattgaag cgcggcgctc atgtgttacg attccttcca ggttatcgac
     3901 cgacatcgcc cctgtatctt cgtgaaagtc ttgccggata gacacgaatt tcaggggcgt
     3961 ttgtttttca gtcgcttaca ctacccccaa cgacaaaacc tgtcgagtct tcccgcaaac
     4021 ccgatccatt taccataact cccgttgtgc gcccccctta aaacgccctc agtcgctttc
     4081 tgtcgcaaaa aacgcaaaaa gacagggaaa caacaggcca gaccaaaaaa acgcgccaga
     4141 attgttttca gggggctgca taactatgca cgaaagcgat tcatgtccgg tttcactcgt
     4201 tttgaacagc gaacaggtca aaacagcaaa ctttctcatc cagaaaaagg tgaactggtg
     4261 cgaaatcttt tcgaactgcg taagacgttg agcacaaaca tagtaagtcg gtttatcggt
     4321 atgtatggat tccattaaca tatcgcctgt attaatattt cctcgttatt gttagaaata
     4381 aaaatgggaa aaatgaatat taactttaaa catatccttc ctttattgtc gtcatttttt
     4441 atttcaggat gcgactctga tggaataagc aaggtaaagt ctttcgtata ttacgacatt
     4501 gacgactcaa tgactgttgg taaagccttc gatacaagga gtgattgcat caatggcaca
     4561 tggagtgaaa aagaggatga tcgaggaaga accatcgtca catatacctg tgatattagt
     4621 gaaagtggcc taaaaataat aaattcagcc attatgcaag aacctgaaga taaggctgaa
     4681 tattctatag cacaaaataa caacagcata aaatcaacca atgaatcact agaggaatac
     4741 aaacagaaaa cgcctctggt ggaaaaaaca aaagaggtca taatagatca tataagaaag
     4801 ctaaacagtg cttttaatga agatccaatg atatatagca aactcacaat tagcccttat
     4861 ttagacagaa tgctatatct aaaggaccat aatgacaatg cgctaaatga aatttgtggt
     4921 gggtacgaat acattcaatc tgccgatagt tacagttggc gtgatgtaag tgaagagtat
     4981 gcaaaagaat catgcgaaaa gcatatatat aaagtatatc aatcgtttaa gaaaaatgca
     5041 tcaccattaa taactaaaaa cttccccggt ttctacgaac gtgtcccccc ctgtgagaac
     5101 gcagatgaat gcat
//
LOCUS       DAZSBF010000262         5044 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZSBF010000262 DAZSBF010000000
VERSION     DAZSBF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.267"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="ACHXWD_003832"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="ACHXWD_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1771
                     /locus_tag="ACHXWD_003833"
                     /pseudo
     CDS             1595..1771
                     /locus_tag="ACHXWD_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1779..1868)
                     /locus_tag="ACHXWD_003834"
                     /pseudo
     CDS             complement(1779..1868)
                     /locus_tag="ACHXWD_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHXWD_003835"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="ACHXWD_003835"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR7622612.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="ACHXWD_003836"
     CDS             complement(3750..4700)
                     /locus_tag="ACHXWD_003836"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR7622613.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="ACHXWD_003837"
     CDS             complement(4839..>5044)
                     /locus_tag="ACHXWD_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HFR7622614.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1316 a         1288 c         1230 g         1210 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttg aattggtacg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAZSBF010000263         4995 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZSBF010000263 DAZSBF010000000
VERSION     DAZSBF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.268"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            243..956
                     /gene="ydiV"
                     /locus_tag="ACHXWD_003838"
     CDS             243..956
                     /gene="ydiV"
                     /locus_tag="ACHXWD_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR7622615.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            1019..2455
                     /gene="selO"
                     /locus_tag="ACHXWD_003839"
     CDS             1019..2455
                     /gene="selO"
                     /locus_tag="ACHXWD_003839"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR7622616.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(2459..2650)
                     /gene="hemP"
                     /locus_tag="ACHXWD_003840"
     CDS             complement(2459..2650)
                     /gene="hemP"
                     /locus_tag="ACHXWD_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR7622617.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2782..3828)
                     /gene="aroH"
                     /locus_tag="ACHXWD_003841"
     CDS             complement(2782..3828)
                     /gene="aroH"
                     /locus_tag="ACHXWD_003841"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR7622618.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3985..4818)
                     /gene="ppsR"
                     /locus_tag="ACHXWD_003842"
     CDS             complement(3985..4818)
                     /gene="ppsR"
                     /locus_tag="ACHXWD_003842"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR7622619.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         1242 a         1154 c         1277 g         1322 t
ORIGIN      
        1 tcgaaaaatg gacggcgatg atacatcgcc gtccatttac tcctgaatac agtagtattt
       61 tgcagacaaa aattccgtaa atggattatt cgtttataat caggataatc taaaaaaagg
      121 acccctgaaa aagtggttaa catggaacaa atgaaagtta ctccttctgt aattgttatg
      181 tcttatggcc tcatcccttt tagccggata ctgaaaaaca tccttcgaga gggacggtta
      241 ccatgaagat ttttttggag aatctttatc attctgattg ctatttcctg ccgataagag
      301 ataaccagca agtccttgtc ggcgttgagt taattaccca tttctccagt gaagatggca
      361 cggtgcgtat cccgacgagt cgggttatag cgcaacttac cgaggagcaa cattggcaat
      421 tattttcaga gcagttggaa ttactaaaat catgccaaca tttttttatt caacataaac
      481 tctttgcctg gcttaattta acgccacaag tggctacatt attattagag agggataatt
      541 acgcaggcga actattaaaa tatccgttta ttgaactgtt gattaatgag aattacccgc
      601 atctcaatga aggaaaagat aatcgcggtc ttttatcctt atcgcaagtg tatccccttg
      661 tgttaggtaa tttgggagca ggcaatagca caatgaaagc ggtttttgat ggtcttttca
      721 cccgcgtcat gctggataaa agttttattc agcaacaaat cacgcaccgc tccttcgaac
      781 cgtttattcg tgcgatccag gcgcaaattt ccccttgttg caattgcatc attgcgggag
      841 gcattgatac ggcagaaata ttggcacaga ttaccccctt cgatttccat gctctccagg
      901 gatgcctgtg gcctgccgtt cccataaatc agataacgac gttggttcag cgataaccct
      961 tctgtttgct ggtgtttaag acgagagtaa ccgtctacac tatcaaacag gaggatctat
     1021 gaccctgtct tttgttaccc gctggcgcga tgaattgcca gaaacctata cagcactttc
     1081 ccctacgcct ttaaataatg cccggctgat ttggcataat accgaactgg ctaacacgct
     1141 gagtattcca tcgtcgctgt ttaaaaatgg cgcaggtgtc tggggcggcg aagccctact
     1201 gcctggtatg tcaccactgg cgcaggttta cagtggtcat cagttcggcg tctgggcggg
     1261 ccaactgggt gatgggcgcg gcattttact cggcgaacaa ctgcttgctg atggcactac
     1321 aatggactgg catctgaaag gtgctggcct gacgccttat tcgcgaatgg gtgatggacg
     1381 ggcggtttta cgttcgacga tacgagaaag tctcgccagt gaggcgatgc attatctggg
     1441 cattccgacg acccgcgcgt taagtatcgt caccagcgat tcgccagtgt atcgggaaac
     1501 ggcggagcca ggcgcgatgc tgatgcgtgt ggcaccaagt catctgcgct ttggtcattt
     1561 cgaacatttt tactaccgcc gcgagtcgga aaaggttcgt cagttagctg actttgctat
     1621 tcgtcattac tggtcacatc ttgaagatga tgaggacaaa taccgtctct ggtttagcga
     1681 tgttgtcgca cgtaccgcgt cgttaattgc ccaatggcag acggtcggct ttgcacatgg
     1741 ggtgatgaat accgacaaca tgtcgctgct gggactgacg cttgattacg ggccgtttgg
     1801 ttttcttgat gattacgaac ccggttttat ttgtaatcac tcggatcatc aagggcgtta
     1861 tagttttgat aatcaacctg ctgtcgcgtt gtggaattta cagcgtctgg cgcagacatt
     1921 gtcaccattt gttgccgtag atgccctgaa tgaggccctg gacagctatc agcaggtttt
     1981 gttgacgcat tatggacaac ggatgcggca gaaactgggc ttcatcacgg agcaaaaaga
     2041 ggataacgcg ctactgaatg aattattcag tctgatggcg cgagagcgca gcgattatac
     2101 ccgcacattc cgcatgctga gtctgaccga gcagcacagc acggcgtcac cgctacgtga
     2161 tgagtttatt gatcgtgcgg catttgatgg ctggtttgcc cgttatcggg ggcgtttgca
     2221 acaagacgag gttagcgata gtgagcgtca gcagttgatg caaagcgtta accccgctct
     2281 ggtattgcgc aactggttgg cgcaacgggc gattgaagcg gcagaaaagg gtgatatgac
     2341 ggaattgcac cgcctgcatg gggcgttgcg aaatcctttc agcgatagag atgatgacta
     2401 tgtcagtcgt ccacctgact ggggtaaacg gctggaagtc agctgctcga gttaaaggct
     2461 acttagttaa cagcagcttg ccagcctgag ttttacggag cagatattct tgcccgtcat
     2521 gatcgataat cagtttgcca tccggaccta acagtgtctg gctggtaatt cgccggatgg
     2581 gctgcgggga agcggccttg tgattggcgt ccgtttcagg cgtgagtttt ctgctatccg
     2641 tataacgcat aaagattaat aataattgat ataacaatga ttcttattat caatattatg
     2701 gcaggccaga gcaagcagta atttgttgag aagacaacaa gttgcgcaga aatgcccatt
     2761 ccgtcaggaa tgggcacgca ttcagaagcg ggtatctacc gcagaggcga gtttttcgac
     2821 caggcgttcg gtatcttccc agcccagaca cgggtcggta atggattgac cgtaagtgag
     2881 cggctgactg ccgacgattt tttgcgttcc ttcgagcagg aaactttccg ccataattcc
     2941 agcaatcgcc gtagagccat tgcggatttg ctgacaaata tcctcacaaa cttctaactg
     3001 gcgacggtgc tgcttctggc agttaccgtg gctgaaatcc accaccagat gttcaggtaa
     3061 atcaaactcg tgcagcgtat cgcaggctgc ggcgatatca tcggcatgat aattcggttt
     3121 tttgccgcca cgcataataa tgtggccata cgggttgccg ctggtctgat agatggtcat
     3181 ctgaccattt ttgtctggcg agaggaacat atggctggcg cgggctgcgc ggatagcatc
     3241 cacagcaatc cgcgtattgc catcggtacc atttttaaaa cctaccggac aggagagtgc
     3301 cgaagccatt tcacggtgga tctgactttc ggtagtacgt gcgccaatcg cgccccaact
     3361 gattaaatca gcaataaact gaccggtcac catatcgagg aactcagtcg cggttgggac
     3421 gcccagctca tttacctgta aaagtaattt gcgcgccagc tccagaccgt gatttacccg
     3481 atagctgccg tttaaatctg gatcggagat tagtcctttc cagccgacaa cagttcgtgg
     3541 tttttcaaaa taggtgcgca ttacgatttc cagccgtgac tggtactggt tgcgcagcga
     3601 ctgcagacgg ggggcgtact ccattgcagc ggtgagatcg tggatcgagc aggggccaat
     3661 aatgaccaac agtcgcttat cttcaccatt gagtattttt tcaattctgc ggcgggagtc
     3721 ggtgacatgg gtggcggcgc caggcgttac gggataccgt agcgcgagtt cggcgggcgt
     3781 taccaggctc tcaatacgcg cagtacggag ttcgtcagtt ctgttcatta caagtctcag
     3841 aaatttgttg cttctacggc aggactgccg ggaagtgatg cgcatcacga taaaccaatc
     3901 gctgctgatt tcaagtgcga ggtgtgtcac acctcgccgg gtggttagca tcataacaaa
     3961 aaaataagct aatgcactag ttctctagta cattcggcga ctaaggccca tgatatcgag
     4021 gatcttggtg gcaatctctt ctaccgaata attggtactg ttaatccacg ggatctgatt
     4081 tttacggtac aacgcttcca cttccgcgac ttccatcctg cactgacgaa gcgaggcata
     4141 gcgactgttc tcccgacgtt cctcgcgaat cgccgccaga cgttccgggt cgatagtcag
     4201 gccgaacaat ttatgctgaa gcggtttgag cgacgcgggt agcaccagat tatccatatc
     4261 gtcggcaata aaggggtagt ttgccgcgcg gataccaaat tgcattgcca gataaagact
     4321 ggtgggggtt ttaccacagc gagaaacacc gagcaggatc acctgagcct gatccagatt
     4381 gcgcaacgaa atgccgtcat cgtgggcgag ggtgtaatca atcgccgcaa tgcgcgcatc
     4441 atatttattg agattattag ggttaaggcc atgggtacga tgagcaatcg gcgttggatc
     4501 cagtttcatc tcttgttgta gcggggcaac cagcgcctga acgatatcct ggcaaaagcc
     4561 ttcactttgc aagatgatgg cgcgaatctc cggcaacacg atggagtaga agaccagcgg
     4621 gcgcacacct gtctggtgat aaattgcgtc aatctgatcc ttcactgccc gtgcacggct
     4681 ctcattttcg acaaacggca gcgtaatgct gctgatagtg acgggaaatt gtgacattac
     4741 tgcgtgtcct aatacctccg cagttattgc cgtaccatca gaaatataaa aaacgtggcg
     4801 atcaacagca ttatccattt tgttcttccc gtgatgcaga cataattcta tgaaagcata
     4861 aattaaaaac gcacagaagc gtaggacgtt atgtctggtt tataaaatga accttcaatt
     4921 ttatttttta tgaaaacagc atttcatttt tatggtttcg tttataccga tggtttatgt
     4981 ggaaattgtc gaaga
//
LOCUS       DAZSBF010000264         4949 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZSBF010000264 DAZSBF010000000
VERSION     DAZSBF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.269"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHXWD_003843"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHXWD_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(796..1248)
                     /locus_tag="ACHXWD_003844"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHXWD_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHXWD_003845"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHXWD_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHXWD_003846"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHXWD_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR7622620.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHXWD_003847"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHXWD_003847"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHXWD_003848"
     CDS             complement(2927..3859)
                     /locus_tag="ACHXWD_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR7622621.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHXWD_003849"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHXWD_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR7622622.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1195 a         1151 c         1160 g         1443 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc acctttttt
//
LOCUS       DAZSBF010000265         4882 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZSBF010000265 DAZSBF010000000
VERSION     DAZSBF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.270"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(358..1401)
                     /locus_tag="ACHXWD_003850"
     CDS             complement(358..1401)
                     /locus_tag="ACHXWD_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7622623.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1813..3025)
                     /locus_tag="ACHXWD_003851"
                     /pseudo
     CDS             complement(1813..3025)
                     /locus_tag="ACHXWD_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(3297..3391)
                     /locus_tag="ACHXWD_003852"
     tRNA            complement(3297..3391)
                     /locus_tag="ACHXWD_003852"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3355..3357),aa:Sec,seq:tca)
     gene            3684..>4882
                     /locus_tag="ACHXWD_003853"
     CDS             3684..>4882
                     /locus_tag="ACHXWD_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR7622624.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
BASE COUNT         1276 a         1069 c         1117 g         1420 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccggaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaaataata taataacaaa
     1741 aatcctccag ccttgtaggc cagaggataa aatacttaat tgaggaaaat atcagggaaa
     1801 agtatttctg acttaacttt cccggctgga gagtgaatca atgtaatcag cgtaccattg
     1861 catcatttca cggcggccat cgagatacag ggcatgatta taggtacccc tgatggaatt
     1921 tttatccaca tgagctaact gcatttcaat ccaggcggaa ttaaagcctt gttcatgcag
     1981 aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt cttcccttat atccaagtaa
     2041 ttcaattact tggttaatgc tttccttact aattggtttg cggggatcat ttctaccgat
     2101 aaagactagg ggatgatgtt ttgagattgg ttcaagctgc ttaaatagca taatgacctg
     2161 ctcagagagc ggcacaatat gaggtcgctt catcttcatt acttctgccg ggatctccca
     2221 caattttgtc tcaaaatcga tatcttccca gcgtgcaaaa cgcagttctt gtgttcgtac
     2281 gccggttaac ataatgatct gagtagctgt cttagtgatt atacttccgg tataacccgc
     2341 cagatcgttg aggaagtggg gaagttcatt agcagtaaga aagggaaaat gtactttttt
     2401 cggtgtagct aaagcactgg caagatcggg ggctgggtta tagtctgccc ggccagtaac
     2461 aattgcatag cggaacacct caccacagcg ctggcggacc ttgcgcattt tctccagagc
     2521 tccacgtttt tccattttac gcagtgcttc cagtaactcc attggtttga tttcggcaat
     2581 agggcgcttg ccaatgtagg gaaaaatgtc tttttcaaag gtatcaatga tttcatcacg
     2641 atagcgtaat gaccaccgat cagcttttga ctggtgccat tcgcgagcaa tagcctcgaa
     2701 cgtatttttc agactcattt gctgcgcgat tttttcttcc ttttttgcct cccctggatc
     2761 gcctccagca gcgaggagtt tcctggcttc actgcgtttc tcgcgagcat cggctagcgt
     2821 tacatcgggg tatacgccaa tagaaagcat cttttcttta ccagcaaagc ggtacttcat
     2881 gcgccagtat ttagaaccat tagtgttaat cagtagatac ataccgccgc catcagcaag
     2941 cttatagggt ttgtctttgg gttttgcggt tttcactttg atatcggtta atgccatgac
     3001 atcctccggc ttttgatgaa ttcatgttcc tgagggggat ttctgccagt aactaacctg
     3061 atacccccaa tacacccctt gtataacaaa gatattgtta aaagccgttg ttatgtatca
     3121 catttttcct attatggggg tgcttgtatt tcaataggaa ataacacccc ctaaagtacc
     3181 cccaaaagaa acttgatatg gggtgaagta ggttgacctc aggatacaaa taaatactga
     3241 tatacaaggg attagaagag atttattgac attgggagag ttcagaagag gtaaactggc
     3301 ggaagatcac aggagtcgaa cctgcccggg accgctggcg gccccaactg gatttgaagt
     3361 ccagccgcct caccggagac gacgatcttc cgcgcctcga ttgctacatg gaggcggggc
     3421 gcattatagc tacttccttg agtttctaca tcccccagat cagttttttc cgcctaatac
     3481 acgcagcgta ttatcacgaa agcattataa ctcatataaa acccatattt tacaatgata
     3541 tatgagcgga attaacgcta ccacgatcac ataaaacaag aatcataaat taataaccag
     3601 atatcggaat attcgctctc acagggatgg tttacaaaat gcgttatcgg tctcagcacc
     3661 cctatagcat caaggaaaag cagatgaaga gtgaagtgtt gtccgttaaa gagaaaattg
     3721 gttatggcat gggagacgcc gccagccaca ttattttcga taacgtaatg ttatatatga
     3781 tgttctttta taccgatatt tttggcattc ctgccggatt tgtcggaacc atgtttttgg
     3841 tcgctcgtgc actggatgcg atttccgatc cttgcatggg gttgttggcc gatcgaacgc
     3901 gctctcgctg gggtaaattt cgtccgtggg tactgtttgg cgcactgcca ttcgggatcg
     3961 tctgtgtact ggcctatagc acgccagatc tcagtatgaa cggcaaaatg atctatgcag
     4021 caattactta caccctactt accttacttt ataccgtcgt caatatccct tactgcgcat
     4081 tgggtggtgt aatcaccaat gacccgactc agcgtatctc gctgcaatcc tggcgttttg
     4141 tgctggcgac cgcgggaggc atgctttcta ctgttctgat gatgccactg gttaatttaa
     4201 ttggcggtga taataaacca ctcggtttcc agggcggtat cgcggtcctt tccgtggtgg
     4261 cattcatgat gctggcattt tgtttcttca ccactaaaga acgcgttgaa gcaccaccta
     4321 caacaacgtc tatgcgggaa gatttacgtg atatctggca aaacgaccag tggcggattg
     4381 tcggtttact aaccattttc aatatcctgg cggtgtgcgt acgcggtggg gcgatgatgt
     4441 attacgtcac atggattttg ggcacgccgg aagtgtttgt cgcttttctc accacttatt
     4501 gcgtgggtaa cctgattggt tccgcactgg caaaacctct gaccgactgg aaatgtaaag
     4561 tcactatctt ctggtggacg aacgccctgc tggcagtgat tagcctcgcg atgttctttg
     4621 ttcccatgca ggccagcatc actatgtttg tcttcatctt cgtgattggt gtgttgcatc
     4681 aactggtgac acctatccag tgggtaatga tgtccgatac cgtcgactac ggcgagtggt
     4741 gcaatggtaa acgcctgacc gggatcagtt ttgctggcac gctgtttgtg ctcaaactgg
     4801 ggttggcctt cggcggcgct cttatcggct ggatgctggc ttatggcgga tatgatgcgg
     4861 cagaaaaagc gcagaacagc gc
//
LOCUS       DAZSBF010000266         4831 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZSBF010000266 DAZSBF010000000
VERSION     DAZSBF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.271"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHXWD_003854"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHXWD_003854"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR7622625.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHXWD_003855"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHXWD_003855"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR7622626.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1072 a         1351 c         1246 g         1162 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttaatta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atattttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat t
//
LOCUS       DAZSBF010000267         4813 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZSBF010000267 DAZSBF010000000
VERSION     DAZSBF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.272"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHXWD_003856"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHXWD_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR7622627.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHXWD_003857"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHXWD_003857"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR7622628.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHXWD_003858"
     CDS             2475..4124
                     /locus_tag="ACHXWD_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR7622629.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..>4813)
                     /locus_tag="ACHXWD_003859"
     CDS             complement(4278..>4813)
                     /locus_tag="ACHXWD_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7622630.1"
                     /translation="MAEANLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKA
                     ILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCHFNCSILTKACMF
                     NTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDN
                     INAVAMKITADIMQHAD"
BASE COUNT         1003 a         1145 c         1332 g         1333 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgt
//
LOCUS       DAZSBF010000268         4805 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZSBF010000268 DAZSBF010000000
VERSION     DAZSBF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.273"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            101..1045
                     /gene="leuO"
                     /locus_tag="ACHXWD_003860"
     CDS             101..1045
                     /gene="leuO"
                     /locus_tag="ACHXWD_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR7622631.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1200..1244)
                     /locus_tag="ACHXWD_003861"
     CDS             complement(1200..1244)
                     /locus_tag="ACHXWD_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622632.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1363..3087
                     /gene="ilvI"
                     /locus_tag="ACHXWD_003862"
     CDS             1363..3087
                     /gene="ilvI"
                     /locus_tag="ACHXWD_003862"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR7622633.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3090..3581
                     /gene="ilvN"
                     /locus_tag="ACHXWD_003863"
     CDS             3090..3581
                     /gene="ilvN"
                     /locus_tag="ACHXWD_003863"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR7622634.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3593..3679
                     /locus_tag="ACHXWD_003864"
     CDS             3593..3679
                     /locus_tag="ACHXWD_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR7622635.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3761..4765
                     /gene="cra"
                     /locus_tag="ACHXWD_003865"
     CDS             3761..4765
                     /gene="cra"
                     /locus_tag="ACHXWD_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR7622636.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1167 a         1097 c         1279 g         1262 t
ORIGIN      
        1 ctttttctta ttttatatgc atgataaatc atattcttca ggattatttc tctgcattcc
       61 aataagggaa agggagttaa gtgtgacagt ggagttaagt atgccagagg tacaaacaga
      121 tcattcagag acggcggagt taagcaagcc acagctacgc atggtcgatc tcaacttatt
      181 aaccgttttc gatgccgtga tgcaggagca aaacattacc cgtgccgctc atgttctggg
      241 tatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg aaggtgatgt ttaatgacga
      301 gctttttgtt cgttatggcc gtggtattca accgactgct cgcgcatttc aactttttgg
      361 ctcagttcgt caggcattgc aactagtaca aaatgaattg cctggttcag gttttgaacc
      421 cgcgagcagt gaacgtgtat ttcatctttg tgtttgcagc ccgttagaca gcattctgac
      481 ctcgcagatt tataatcaca ttgagcagat tgcgccaaat atacatgtta tgttcaagtc
      541 ttcattaaat cagaacactg aacatcagct gcgttatcag gaaacggagt ttgtgattag
      601 ttatgaagac ttccatcgtc ctgaatttac cagcgtgcca ttatttaaag atgaaatggt
      661 gctggtagcc agcaaaaatc atccaacaat taagggcccg ttactgaaac atgatgttta
      721 taacgaacaa catgcggcgg tttcgctcga tcgtttcgcg tcatttagtc aaccttggta
      781 tgacacggta gataagcaag ccagtatcgc gtatcagggc atggcaatga tgagcgtact
      841 tagcgtggtg tcgcaaacgc atttggtcgc tattgcgccg cgttggctgg ctgaagagtt
      901 cgctgaatcc ttagaattac aggtattacc gctgccgtta aaacaaaata gcagaacctg
      961 ttatctctcc tggcatgaag ctgccgggcg cgataaaggc catcagtgga tggaagagca
     1021 attagtctca atttgcaaac gctaactgaa tgcagaatag gtcagacatg aatgtctggt
     1081 ttattctgta ttttttattg aatgtagaat tttattctga atgtgtgggc tctctatttt
     1141 aggattaatt aaaaaaatag agaaattgct gtaagttgtg ggattcagcc gatttattat
     1201 caatttaatc cttcgtaatg gaggatttta tcgtttcttt tcacctttcc tcctgtttat
     1261 tcttattacc ctgcgtttat gtctctggct gccaattgct taagcaagat cggacggtta
     1321 atgtgtttta cacatttttt ccgtcaaaca gtgaggcagg ccatggagat gttgtctgga
     1381 gccgagatgg tcgtccgatc gcttatcgat cagggcgtta aacaagtatt cggttatccc
     1441 ggaggcgcag tccttgatat ttacgatgca ttgcataccg tgggtggtat cgatcatgtt
     1501 ttagttcgtc atgagcaggc ggcagtgcat atggccgatg gtctggcgcg cgcgaccggg
     1561 gaagtcggcg tcgtgctggt aacgtcgggt ccaggggcga ccaatgcgat tactggcatc
     1621 gccaccgctt atatggattc cattccatta gttgtccttt ccgggcaggt agcgacctcg
     1681 ttgataggtt acgatgcctt tcaggagtgc gacatggtgg ggatttcgcg accagtggtt
     1741 aaacacagtt ttctggttaa gcaaacggaa gacattccgc aggtgctgaa aaaggctttc
     1801 tggctggcgg caagtggtcg tcctggacca gtagtcgttg atttaccgaa agatattctt
     1861 aatccggcga acaaattacc ctatgtctgg ccggagtcgg tcagtatgcg ttcttacaat
     1921 cccactacta ccggacataa agggcaaatt aagcgcgctc tgcaaacgct ggtagcggca
     1981 aaaaaaccgg ttgtctacgt aggcggtggg gcaatcacgg cgggctgcca tcagcagttg
     2041 aaagaaacgg tggaggcgtt gaatctgccc gttgtttcct cattgatggg gctgggagcg
     2101 tttccggcaa cgcatcgtca ggcactgggc atgctgggaa tgcacggtac ctacgaagcc
     2161 aatatgacga tgcataacgc ggatgtgatt ttcgccgtcg gggtacgatt tgatgaccga
     2221 acgacgaaca atctggcaaa gtactgccca aatgccactg ttctgcatat cgatattgat
     2281 cctacttcca tttctaaaac cgtgactgcg gatatcccga ttgtggggga tgctcgccag
     2341 gtcctcgaac aaatgcttga actcttgtcg caagaatccg cccatcaacc actggatgag
     2401 atccgcgact ggtggcagca aattgaacag tggcgcgctc gtcagtgcct gaaatatgac
     2461 actcacagtg aaaagattaa accgcaggcg gtgatcgaga ctctttggcg gttgacgaag
     2521 ggagacgctt acgtgacgtc cgatgtcggg cagcaccaga tgtttgctgc actttattat
     2581 ccattcgaca aaccgcgtcg ctggatcaat tccggtggcc tcggcacgat gggttttggt
     2641 ttacctgcgg cactgggcgt caaaatggcg ttgccagaag aaaccgtggt ttgcgtcact
     2701 ggcgacggca gtattcagat gaacatccag gaactgtcta ccgcgttgca atacgagttg
     2761 cccgtactgg tggtgaatct caataaccgc tatctgggga tggtgaagca gtggcaggac
     2821 atgatctatt ccggccgtca ttcacaatct tatatgcaat cgctacccga tttcgtccgt
     2881 ctggcggaag cctatggtca cgtcgggatc cagatttctc atccgcatga gctggaaagc
     2941 aaacttagcg aggcgctgga acaggtgcac aataatcgcc tggtgtttgt tgatgttacc
     3001 gtcgatggca gcgagcacgt ctacccgatg cagattcgcg ggggcggaat ggatgaaatg
     3061 tggttaagca aaacggagag aacctgatta tgcgccggat attatcagtc ttactcgaaa
     3121 atgaatcagg cgcgttatcc cgcgtgattg gccttttttc ccagcgtggc tacaacattg
     3181 aaagcctgac cgttgcgtca accgatgatc cgacattatc gcgtatgacc atccagaccg
     3241 tgggcgatga aaaagtactt gagcagatcg aaaagcaatt acacaagctg gtcgatgtct
     3301 tgcgcgtgag tgagttgggg cagggcgcgc atgttgagcg ggaaatcatg ctggtgaaaa
     3361 ttcaggccag cggttacggg cgtgacgaag tgaaacgtaa tacggaaata ttccgtgggc
     3421 aaattatcga tgtcacaccc tcgctttata ccgttcaatt agcaggcacc agcgataagc
     3481 ttgatgcatt tttagcatcg attcgcgagg tggcgaaaat tgtggaagtt gctcgttctg
     3541 gtgtggtcgg actttcgcgc ggcgataaaa taatgcgttg agaatgatct gaatgcgcaa
     3601 tttgcagccc aacatgtcac gttgggcttt ttttgcgaaa tcagtgggaa cctggaataa
     3661 aagcagttgc cgcagttaat tttatgcgct tagatgttaa tgaatttaac ccataccagt
     3721 acaatggcta tggtttttac attttacgca aggggcaatt gtgaaactgg atgaaatcgc
     3781 tcggctggcg ggagtgtcgc ggaccactgc aagctatgtt attaacggca aagcgaagca
     3841 ataccgtgtg agcgacaaaa ccgttgaaaa agtcatggct gtggtgcgtg agcacaatta
     3901 ccacccgaac gccgtggcag ctgggcttcg tgctggacgc acacgttcta ttggtcttgt
     3961 gatccccgat ctggagaaca ccagctatac ccgcatcgct aactatcttg aacgccaggc
     4021 gcggcaacgg ggttatcaac tgctaattgc ctgctcagaa gatcagccag acaacgaaat
     4081 gcgctgtatt gagcaccttt tacagcgtca ggtcgatgcc attattgttt cgacgtcgtt
     4141 gcctcctgag catccttttt atcaacgctg ggctaacgac ccgttcccga ttgtcgcgct
     4201 ggaccgcgcc ctcgatcgtg aacacttcac cagcgtggtt ggtgccgatc aggatgatgc
     4261 cgaaatgctg gcggaagagt tacgtaagtt tcccgccgag acggtgcttt atcttggcgc
     4321 gctgccggag ctttctgtca gcttcctgcg tgaacaaggt ttccgtactg cctggaaaga
     4381 tgatccgcgc gaagtgcatt tcctgtatgc caacagctat gagcgggagg tggctgccca
     4441 gttattcgaa aaatggctgg aaacgcatcc gatgccgcag gcgctgttca caacgtcgtt
     4501 tgcgttgttg caaggggtga tggatgtcac gctacgtcgc gatggtaaac tgccttctga
     4561 cctggcaatt gccacctttg gcgataacga actgctcgac ttcttacagt gcccggtgct
     4621 ggcagtggct caacgtcacc gcgatgtcgc agagcgtgtg ctggagattg tcctggcaag
     4681 cctggacgaa ccgcgtaagc caaaacctgg tttaacgcgc attaaacgta atctctatcg
     4741 ccgcggtgtg ctcagccgta gctaagccgc gaacaaaaat acgcgccagg tgaatttccc
     4801 tctgg
//
LOCUS       DAZSBF010000269         4784 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZSBF010000269 DAZSBF010000000
VERSION     DAZSBF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.274"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHXWD_003866"
     tRNA            6..81
                     /locus_tag="ACHXWD_003866"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHXWD_003867"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHXWD_003867"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHXWD_003868"
     CDS             complement(299..1057)
                     /locus_tag="ACHXWD_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR7622637.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHXWD_003869"
     CDS             complement(1065..2168)
                     /locus_tag="ACHXWD_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR7622638.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHXWD_003870"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHXWD_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="ACHXWD_003871"
     CDS             complement(3427..4452)
                     /locus_tag="ACHXWD_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7622639.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1311 a         1236 c         1143 g         1094 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gcta
//
LOCUS       DAZSBF010000270         4782 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZSBF010000270 DAZSBF010000000
VERSION     DAZSBF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.275"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACHXWD_003872"
     CDS             complement(56..817)
                     /gene="kduD"
                     /locus_tag="ACHXWD_003872"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR7622640.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(882..1718)
                     /gene="kduI"
                     /locus_tag="ACHXWD_003873"
     CDS             complement(882..1718)
                     /gene="kduI"
                     /locus_tag="ACHXWD_003873"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR7622641.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(2005..3186)
                     /locus_tag="ACHXWD_003874"
     CDS             complement(2005..3186)
                     /locus_tag="ACHXWD_003874"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR7622642.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3441..4670
                     /locus_tag="ACHXWD_003875"
     CDS             3441..4670
                     /locus_tag="ACHXWD_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7622643.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1239 a         1215 c         1052 g         1276 t
ORIGIN      
        1 tacaggtata aaaaaccctg ccatgaggca gggtcataaa agtaagagga atgaattaac
       61 gagccagcca accgccatct acggcaatgg tataaccatt cacataatct gaagcgctgg
      121 aggcaaggaa cactatcggc cccatcaggt cactcggcag tccccaacga ccagctggaa
      181 tgcggtcgag aatttccgcg ctacgttgtt catctgcccg tagttgttga gtattgttgg
      241 tcgccatgta acccggagct atcgcattaa cattaatgtt gtgtttagcc cattcgttcg
      301 ccatcaatcg cgtcacgccc atcacgccgc tttttgatgc ggtataagaa ggcacacgga
      361 tcccgccctg gaaggagagc attgacgtga tattgataat cttgccgcca ttgcgttgcg
      421 cgataaagtg tttcgccgct gcctgagaca tgaagaatac gctcttgata ttcaggttca
      481 tgacatcgtc ccagtctttt tcgctgaact cgagagcatc ttcgcggcga atcaatccgg
      541 cgttattcac caggatatca atatgaccaa actcagctac cgcgcgatcc agcagtgctg
      601 gaatgccatc aatctttcgc agatcggcgg tcaggcttaa aaaacgacgc cccagcgccg
      661 tgacctgctt gatggtttca gtcggttcaa cgatgttaat gccaacaatg tcacagcccg
      721 cttgcgccag ccccaacgcc atcccctggc ccagtccagt atcacaacca gtgacgaccg
      781 caactttacc ttcgagagaa aatgcactta aaatcataac aataccttat tcgtttatgc
      841 cgacaactag cgcaaatctt taacggccac tatgccgaca actagcgcaa atctttaacg
      901 gccacatggt ccatatcatc aaagacctgg ttttcaccaa ccatgcccca gataaaggta
      961 taagctttgg ttccgacgcc ggaatggatc gaccagctgg gggagatcac cgcctgctcg
     1021 ttatgcatca caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg
     1081 tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgagt gtgacacggc
     1141 atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa ttggcaggtt
     1201 tccagcacat ccgggacaaa atatttgtta attgtgcgac ggttactggt gaggttatcg
     1261 cctaacgtga ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc
     1321 gcgggtgcgc aattgtaata aaactttgcc ggagtggcgg tatcaatact ggcaaagaca
     1381 acttcttttg cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattgg
     1441 ccatcgacag taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca
     1501 aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt
     1561 atcggcataa tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca
     1621 tcggcgacaa ataccttttc aaccaaaaat tcattgcgca gcccttgggt atccagcgtt
     1681 tttgcgtgcg cactgtggat gctctgtctt acgtccacat caacctccga taatcagtga
     1741 agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata
     1801 tgcccatggg ctagggtttt caattaaaaa taaaacaatg tttcattttt aagttaagga
     1861 ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc
     1921 agaaaatgta tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt
     1981 tatcggtaag gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgccctg
     2041 gcccccgccg atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg
     2101 aaccagagaa accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc
     2161 gccaccattg acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc
     2221 ctgtgcagca aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac
     2281 acgctccagg caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac
     2341 accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt
     2401 ggcttcgctc atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc
     2461 ggtcacagaa ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc
     2521 gtcagtgcgt ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat
     2581 taccgggacg atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg
     2641 cgagctaagt gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt
     2701 ttcggcggtg acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag
     2761 actgtcaacc aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg
     2821 tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg
     2881 tatcgcctga gtagccagat gcagtgcttt aagtccggaa ccgcaaacgt cattaatcgt
     2941 gattgcagaa acgctattag gaagaccacc tttaatagcc gattgccttg ccggattctg
     3001 ccctgcccct gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac
     3061 gccggtacgt tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc
     3121 cgctaacgca ccacgaaagc agccgatagg tgtccgtaac gccccgacaa tcacaacgtc
     3181 tttcatcaca acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta
     3241 tcttaattgt ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca
     3301 gagtcataac tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagataag
     3361 gcgttaatct cccatactta acctggttta tggtaaattg ccctccattt tgtttaattt
     3421 gtagatgata cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt
     3481 tcgcgctcta cggcacagcc gttggtgcag gcacgctctt ccttcctatt cagttaggtt
     3541 cggcaggggc tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattggc
     3601 cacataaagc cttatgccag ttcatcctct catcaaaaac atcagcatgt gaagggataa
     3661 cgggcgcggt aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact
     3721 tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg
     3781 aacagctggc aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg
     3841 ttgtattaat tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg
     3901 gatttttggt attcccattg attgcctatt tcttatttct ttccatttac cttgtcggta
     3961 gttggcaacc tgatctatta acgacccagg tagagttcaa tcagaatacc cttcaccaga
     4021 tatggatatc gattcccgtg atggttttcg cctttagcca tacgcccatt atttctacgt
     4081 ttgccataga cagacgtgaa aaatatggcg aacacgctat ggataaatgc aaaaaaatta
     4141 tgaaagtcgc ttatctcatc atctgcataa gtgtactgtt ctttgtcttt agctgcctgc
     4201 tttctattcc accttcgtat attgaagcgg ctaaagaaga aggggtcacc attttatcgg
     4261 cgctttctat gctgccgaac gccccagcat ggttgtcaat ttccgggatt attgtcgcag
     4321 tagttgcgat gtcgaaatca ttcctgggta cgtactttgg cgttattgaa ggtgccacgg
     4381 aggtcgtcaa aacaacacta cagcaggttg gtgtaaagaa aagtcgtgca tttaaccgcg
     4441 cactatcaat tatgttggta tcgctgatta ccttcattgt ttgttgcatt aacccgaacg
     4501 cgatttcgat gatttatgcg atcagcggcc cgctcattgc catgatactt ttcatcatgc
     4561 ctacgttgtc aacgtatctg atcccggcgc ttaaaccctg gcgctccatc ggaaatctga
     4621 ttacgctgat cgtgggtatc ctgtgcgtat cggtaatgtt ctttagctaa tatccgttgt
     4681 ggatggttca aaattgctac aattttgaac cattccattt ttcataatat tcatttgtta
     4741 ttattccgcc ctatcacttc tctcgtcata tcctagaatt at
//
LOCUS       DAZSBF010000271         4760 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZSBF010000271 DAZSBF010000000
VERSION     DAZSBF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.276"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHXWD_003876"
     CDS             <1..279
                     /locus_tag="ACHXWD_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR7622644.1"
                     /translation="YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWK
                     NRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            620..2272
                     /gene="ushA"
                     /locus_tag="ACHXWD_003877"
     CDS             620..2272
                     /gene="ushA"
                     /locus_tag="ACHXWD_003877"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFR7622645.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIREHSPLDAASYEPKGEVSWQ"
     gene            complement(2283..2453)
                     /locus_tag="ACHXWD_003878"
                     /pseudo
     CDS             complement(2283..2453)
                     /locus_tag="ACHXWD_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2586..2705)
                     /locus_tag="ACHXWD_003879"
                     /pseudo
     CDS             complement(2586..2705)
                     /locus_tag="ACHXWD_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2779..3327)
                     /locus_tag="ACHXWD_003880"
                     /pseudo
     CDS             complement(2779..3327)
                     /locus_tag="ACHXWD_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="ACHXWD_003881"
                     /pseudo
     CDS             complement(3390..4699)
                     /gene="ltrA"
                     /locus_tag="ACHXWD_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1273 a         1151 c         1111 g         1225 t
ORIGIN      
        1 tacacacagc ggcttaagga agccggatta ctggcatcaa caggaagtac aggcgactcg
       61 tatgacaacg cgatggcgga gagcatcaat ggtctttaca aagcggaggt aatacaccgt
      121 aagagctgga aaaaccgtgc agaagtggaa ctggccacac tcacgtgggt ggactggtat
      181 aataatcgac gattgctgga aaggctgggc cacatccctc cggcagaagc agaaaaagct
      241 tattatgctt ctatcggaaa cgatgatctg gcagcctgag ttcacagata aaacattctc
      301 caggaaactt ggggcggttc acgtcagtat cagaccatat taattgctga aactgataaa
      361 catcaaacct tatagaaggg tgaatgctga aagggggcta tggtgatcga cgtgatgaca
      421 acgacaggta agaccgtgac ctgataaacc tgaattgtgt cctagacata aagtccgtga
      481 agggaataag gctcaggtca gcgaatctta tagtggcccg cagctctgac tgcaataagg
      541 ccggatacag agcagcaact actcgtcgat gtcagaacaa cggaagcctt gcaaacgggg
      601 atgcgttcat acaggtacaa tgataccgtt aaaaaaaaac attacgctaa taatgttcac
      661 cctgtcatta ctgacaggaa atcctgctat agcgtatgaa acagataaag tatataaaat
      721 aaccgttctg catacaaatg accaccatgg acacttttgg cgtaacaatc atggtgagta
      781 cgggttatcc tcccaaaaaa ctctggtgga caatatccgg cagaaagtaa taaataatgg
      841 cggaagtgtt ctattattat ctggtggtga tattaacacc ggtgtacctg aatcagacct
      901 tcagaaggct gaacccgata ttcgtggaat gaatctaatt ggttatgatg caatggccgt
      961 cggtaatcat gaatttgata atcctctaaa tatattacgt cagcaggaaa aatgggccac
     1021 atttcctttc ctttctgcta acatatatca aaaaagtaca gggaggcgtt tattcagtcc
     1081 atggaaaatt ttcatccgtc agaatttgaa aattgcagtc attggcttga cgacagatga
     1141 tactgcgaaa acaggaaatt ctgaatattt tactgatatt gagttccgtc aacctgctgc
     1201 tgaggcgagg agtgtcattg atgaacttaa tcagcaggaa aagccagata ttattattgc
     1261 ggcaacacat atgggtcact atgacaatgg cgagtcaggt tcgaatgctc ctggtgatgt
     1321 tgaaatggct cgcagtttgc cgactggctc attagcaatg attgtgggag gacattcaca
     1381 agctcctgtc tgcatggcca gcgataataa aaaacagtgg aattatattc cggggacaac
     1441 ctgtgtacca gataaacaaa atggcatctg gattgtacaa gcgcatgagt ggggaaaata
     1501 tgtcggtcag gctgattttg aattctgtaa cggtacaatg aaactggtta attatcagtt
     1561 acacccagtt aatctcaaaa tgagaattac cagagaagat ggaaaaacgg aattttcttt
     1621 ttacaccccc gaaattactg aagatccaca gatgttatcc ttactgacac cattccagaa
     1681 taaaggcaaa gcacaactgg atgtgaaggt tggggttgtg aacggacgtc tggaagggga
     1741 cagaagtaaa gttcgttttg ttcagaccag tatgggccat ctgattctga gcgctctgac
     1801 agaacgtatt gacgcagatt ttgctgttgt cagtggtggg gaaattcgag attccataga
     1861 gagtggtaac atcacataca aagatatact gaaagttcag ccatttggta atacggtagt
     1921 aagtattgat ttgacgggga aagaagtagc tgactaccta gctacagtgg cacagatgaa
     1981 accggattcc ggagcctacc cacaattttt gaataccagc tttgtagtca aaaaaggaaa
     2041 aatcgaaatg ctaaaaataa aaggtaaatc ggttgacctt aacaaaaaat atcgtatgac
     2101 aacatttagc tttaatgcta ctggtgggga tggctatcca cgtattgata atagacccgg
     2161 atacataaat accggattta ttgatgctga agtacttatc gaatatatcc gggaacattc
     2221 accgctggat gctgcttcat atgaaccaaa aggtgaagtc agttggcagt gataacgtaa
     2281 ttgacgatca tcacgtgttt tttgctgaca gtcgctgtga ctttgtgaaa gtagtctctg
     2341 cggatgtttg cgatatggga atgcagttgc tgtattttgt gtttctgctt ctgccagttg
     2401 ttgctgaatt taaccttgcg gcttaactga tgttggagcg ctgccagctt cttcgtcaat
     2461 ttcgctgatt aaagtttatt cattatggca tgatctgcgt aagcggctcg tcagaaccgt
     2521 attgatattt actaagagct cagatcaact ttccagggca acagatcgcg tacccggttt
     2581 gccggtcagt cctggatatg ctcaatgacg taacgcagcc atttttctgg ctccacattg
     2641 ttcagacggc atgtgccgat cagcgagtac aacaccgccg catgttcgcc accgctatcg
     2701 gaaccccgcc ggagagagtt tcactctccg gctgctcacg gaaccgtacg tgacactctc
     2761 gcgtcatacg gctcttgatc actcattcga tgcacgtagt aaataccagt gggcaaatag
     2821 cttcggatac tgtttcctta ccagcgccag ctttcgctga gccttccggt tcgaaagtct
     2881 gtatttagac tgcatccact taagcaaacg tgactgcatt gcactccaca gtcgatagct
     2941 gaagtttctg gaccagaact ttccatagta ctcaatccag cctctcacta tcgcattgta
     3001 gcgcctcgca aaatccagca aactctcagc tgttgagcga tgtatacgcc acttcttgat
     3061 tgtttctgtt attttgcaca ttgctgcatt tgatgcaccc ggcatacatt ttctgtacaa
     3121 ttcgcctttg aaattcttca gagtacgcac tttgaagtca tatccgagaa aggagaaact
     3181 cgtagcgacg tttctccttt taaacgtgtc aatgtaggcg atattcgtct tccctgcgtt
     3241 caggaccaac ccgacttccg agaagcgcac cgataacctg ttcttcagcc ttgttgcatc
     3301 actcattcgt gaacaatgta ctacaagtcg agtcacccga gggaacctcc ccctctggtg
     3361 ctcacggaac cggacgtgaa cctctcagct catccggctc ttctcattga ggcctgtccc
     3421 catagtaacg gccagtgtgc aaacaacctc gtttcactcc gcgcaacacg ggccagccag
     3481 tggctggccc gtcgtcggtg gcccctaagt cgcttgtgtt tacggggcag ccataacacg
     3541 agtttgcgat cgatttgcct cagcgtcaga tacagtgctg atttataaaa agcaccgtag
     3601 tactttatcc agcctctgat tttcgcattg aacatatgcg ccagctcgta caaggacttg
     3661 tcactgcgat tctcctcacc tcctgacgga ttgcttttcc tgctctggca ctcatcgccg
     3721 gactgaagtt aacgaaggtc ttaccccagc gattcatcga cagacgcggc cggaatgtat
     3781 agcccagaaa gtcgaagctc gtttccgggt agtccccccg tcggtcagca tccttacagt
     3841 aaactattcg tgttttttgc ggatgcagct gcagcccgca ttgcgcaagc cgctgcctca
     3901 gtccacttat cagagcgtca gcctgccact ggctgtgaca gtggcataca acatcatcgg
     3961 cgtacctttc gaagggaacg ccgggaaact gacgttgcat ccacatatca aaggcatagt
     4021 gcaggaacag atttgagagc aggggggaga ttacgccgcc ctgcggcgtt cctcgacccc
     4081 gtgccagcat tgctccatcc ggcatttgca ccggtgcctt cagcctgcgc tcaatgtaca
     4141 tcaccaccca gcgtgcctga gtatgatgct gcactgtctt cagtagcagg tcatgatcga
     4201 tatcatcaaa gaagccttta atatcgactt ccaccaccca gtcaaacttc cagcagcgtt
     4261 tcctcgcctg cgcaatcgcc tggtgtgcgg atttacccgg tcgataacca tacgagtctg
     4321 catggaacag gggttccagt accggctcga gtatctgctt aacgaccatc tgggcgatcc
     4381 ggtccgacac ggccggaata ccgagcggac gtacaccacc gtcagctttg ggaatgtcca
     4441 cacgccttac cgcctgcggc atataactgc cggaagccat tctgttccac agtttgtaga
     4501 gattgtctgt aacattctcg tcaaacccgg ccagtgtctg cccgtcaatg cctgccgccc
     4561 cacggttggc ttttactcgt ttataagctt cccatacctg tcgtttactt acaggaaatg
     4621 gctttgccga ttttacagca gtcctcccat cggcattgtc gcccgtggtt gccgccgcac
     4681 catcagcaca atggggcaag ccctttgctc cggacccatt acggtccttc accgctacta
     4741 cggtttactc cgtccctgta
//
LOCUS       DAZSBF010000272         4716 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZSBF010000272 DAZSBF010000000
VERSION     DAZSBF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.277"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            99..1073
                     /gene="hemB"
                     /locus_tag="ACHXWD_003882"
     CDS             99..1073
                     /gene="hemB"
                     /locus_tag="ACHXWD_003882"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR7622646.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1179..2030)
                     /gene="tauD"
                     /locus_tag="ACHXWD_003883"
     CDS             complement(1179..2030)
                     /gene="tauD"
                     /locus_tag="ACHXWD_003883"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR7622647.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2027..2854)
                     /gene="tauC"
                     /locus_tag="ACHXWD_003884"
     CDS             complement(2027..2854)
                     /gene="tauC"
                     /locus_tag="ACHXWD_003884"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR7622648.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2851..3618)
                     /gene="tauB"
                     /locus_tag="ACHXWD_003885"
     CDS             complement(2851..3618)
                     /gene="tauB"
                     /locus_tag="ACHXWD_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR7622649.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3631..4593)
                     /gene="tauA"
                     /locus_tag="ACHXWD_003886"
     CDS             complement(3631..4593)
                     /gene="tauA"
                     /locus_tag="ACHXWD_003886"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR7622650.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1075 a         1379 c         1220 g         1042 t
ORIGIN      
        1 cattctcttc ttgtcaacat cacgacaact ttcgtaaaac actcctgccc cgcttcaggt
       61 atactgtgca ccttaattcc acaaacatca ggcagaccat gacagactta atccaacgcc
      121 ctcgtcgcct acgcaaatct cctgcgctgc gcgctatgtt tgaagagaca acacttagcc
      181 ttaacgacct ggtgttgccg atctttgttg aagaagaaat tgacgactac aaagccgttg
      241 aagccatgcc aggcgtgatg cgcattccag agaaacatct ggcacgcgaa attgaacgca
      301 tcgccaacgc cggtatccgt tccgtaatga cttttggcat ctctcaccat accgatgaaa
      361 ccggcagcga cgcctggcgg gaagatggac tggtggcgcg tatgtcgcgc atctgcaagc
      421 agactgtgcc agaaatgatc gtcatgtcag acacttgttt ctgtgaatac acttctcacg
      481 gtcactgcgg tgtgctgtgc gagcatggcg tcgacaacga cgcgactctg gaaaatttag
      541 gcaagcaagc cgtggttgca gctgctgcag gcgcagactt catcgcccct tccgccgcga
      601 tggacggcca ggtacaggcg attcgtcagg cgctggacgc tgcaggcttt aaagatacgg
      661 cgattatgtc gtattcgacc aagttcgcct cttcctttta tggcccgttc cgtgaagctg
      721 ccggaagcgc attaaaaggc gaccgcaaaa gctatcagat gaacccaatg aaccgtcgcg
      781 aggcgattcg tgaatcactg ctggatgaag cccagggcgc agactgcctg atggttaaac
      841 ctgctggagc gtacctcgac atcgtgcgtg agctgcgtga acgtactgaa ttgccgattg
      901 gcgcgtatca ggtgagcggt gagtacgcga tgattaagtt cgccgcgctg gcgggtgcta
      961 tagatgaaga gaaagtcgtg ctcgaaagct taggttcaat taagcgtgcg ggtgcggatc
     1021 tgattttcag ctactttgcg ctggatttgg ctgagaagaa gattctgcgt taattttttt
     1081 cctctcagaa agggggagtt aacctgttga aagtacggtg aacgccccct caccctaacc
     1141 ctctccccaa gggggcgagg gaaccgtccg cactcgtatc accccgcccg ataaaacggt
     1201 ttatccccaa ggatcgtcgc ccgatgcata atccgccgct gtggcaggta atcggcattg
     1261 gcatagtgct gagtcacgcg gttatcccaa atcgcaatat cattcggttg ccagcgccaa
     1321 cgcacctgaa actccggttt agtgatatgg gcgaataaaa agcctaacaa ggcttcgctc
     1381 tctttctcgc tgacatcgac aattcgcgta gtaaagcctt cattcacaaa cagcgcctgt
     1441 ttaccgctca ccggatgcgt tcgcaccacc ggatgtagca acggaggatt tttcgcgacc
     1501 gcctcgcgcc agcgttgatg ttcctcctcg gttttgcggt atttgtattc cgggaacgat
     1561 ttacggaaat catgctccgc acgcagcccg ctcagcagct ggcggaaggg aacagagagc
     1621 gcctcatagg ccgcaatacc gctggtccag agcgtatcac cgccggtcga aggtaactct
     1681 ttagctgcca gaatcgcccc tgcgggtggc gtttcaataa atgtcacatc ggtatgccag
     1741 ttgtcgttat ccggcggatt atcgttatgg gtatccagca cgatgatctc gtcaacccct
     1801 tcggcatgag ggtaaacagg gtgaatatgc agttcgccaa aacgctgggc cagcgcgcgt
     1861 tgctgctgcg gcgtaatagc ttgatcgcgc agaaatacca cctgatagcg cagcaccgca
     1921 tggtaaagct gttcaaactg attatcgctt aacgggcgcg tcaggtcggc acccgaaatt
     1981 tgtgcgccga tatacggccc cagcggggta atactcagac gttcactcat tgtacttctc
     2041 catgccaggg cgtcagacgg cgctgtaacg cgcgcagacc cagttctaaa agaaaggcga
     2101 taatcgcaat caccgcgatc cccgccagta ccacgtcagt cgcgagaaat tcacccgctg
     2161 actgaaccat aaatcctaaa ccgcgtgtcg cggcaatcag ctccgccgct accagcgtag
     2221 accagcccac acccagacca atgcgtaatc cggtgaggat ttccggcagc gcgccgggca
     2281 aaatgacaaa ccacagcacc tgcgcacggc tggcacccag cgactgggcg gcacgaatgc
     2341 gaacctgctg cgcgcttttc acccccgcca gcaccgacat cgccaccggt gcaaaaatcg
     2401 ctaaatagat caataagatc ttcgaggttt caccaatacc aaaccagatc accatcagcg
     2461 gcaaataagc cagcggcggc accggacgat aaagttcgat taccggatcc agaatgccgc
     2521 gcaccgtcgg gcttagcccc atggcgatcc cgaccggaat accgatcacc accgccgcga
     2581 gtagcgccag cacaatgcgc gtcaggctgg ctgccagatg ctgccacagc gtggcgtcca
     2641 taaatccctg cggtccggca atggtgagca gttttgccag tacttgttgc ggcggcggca
     2701 aaaacagcgg gctaatcagt tgcagcgccg ccaccgtcca ccatacagtg agtaaaaccg
     2761 ctaacgtgcc aatgcttaag gtcacctgac gcgagagcgg ccagcgccat ttcagccgcc
     2821 gcgaatgcag tttttcatta atgagcacac tcatgagaac gcctcccgtt gctcaaatac
     2881 gcggcttaaa acatattcgc gcatggcgat gaattgtgga tcggacttga tgctgcggct
     2941 cgactctccc gcaacaaagc ggcgagcaaa gttgagcggc agccgctcca gcacacggcc
     3001 agggccggat gaaagcagaa ccagttcagt cgccataaac accgcttctt ctatatcgtg
     3061 ggtaatcaac agcacctgct tgcccgtctc ctgccagagt ttcagcagca gagtttgcat
     3121 ctggtcgcgg gtgaaggcgt ccagcgcacc aaacggttcg tcgagtaata acagctgggg
     3181 attcgccgcc agcgcgcggg caatccccac ccgctgacgt tgaccaccgg aaagctgcca
     3241 gatatagcgt ttttctgcgc cttccagccc cacttttttc agcatctggt gcgcgatttc
     3301 cagtcgctgc attttctcta tacctgccaa ttgcaggccg aacgccacgt tgtcctgtac
     3361 attgcgccac ggtagtagcc cttcattctg aaaaaccacc ccgcgctctg ctcccggtcc
     3421 ctcaatacgc ttacccgcca gttgaatgct gccatgctga taaggcacaa aaccggcaat
     3481 cagattcagc agggtggttt taccgcagcc ggacggcccc agcaccacca gtagctcgcc
     3541 gctttccagc gtcaggttga tatcttccag tgccggtttg ccgccataat cggcatagag
     3601 atgagagatt tgcagcatct gcgcctcctt ttattgcacg aagcgcgagg taacgtactg
     3661 gctgtaatca ttcgctacag ccgggacctt gccctgctct ttcaaaaact gcgcggtgtc
     3721 gatgattgct ttgttcaccg gtccggtcag ttctgccgtt tgttgctgcg gcgtcagata
     3781 ggtattcccc ttcaccagcc ccggaacgtc accttccggc acgccgctta aacgcgccag
     3841 tttgctgatg ttttccggct gtttcagcca cgcgtctggg ttagcaatgt acggttgctg
     3901 agcatcgatg gcgcttttag cgaacgcttt cacgacctca ggatgtttct cggcaaaatc
     3961 tttgcgcacc acccagacgt ccagcgttgg cgcaccccac tgcccgacct gttcagaatc
     4021 ggtcaacacc ttgccgtctt tttccagggc gttaaccgcc ggtgcccaga cataagcacc
     4081 atcaatatca ccccgctgcc atgcggcgat aatcgcgggc ggctgcaggt tcacaatctc
     4141 cacttgccct ggtttaatcc cccagtgttt cagcgccgcc agcaggctgt agtgggtggt
     4201 ggagataaac ggtacggcaa tgcgtttgcc aatcaaatct tcgggtttgc tgatagtttt
     4261 ctttaccacc agcgcttcgg agttacccag ttttgacgcc agcaagaaga cttcaatcgg
     4321 cacctgttgg ctggctgcaa ccgctaatgg gctggaaccg aggttgccga tttgcacgtc
     4381 gccagaagcc agcgcccgca cgatgctggc tccgctgtca aacttacgcc agtccacgct
     4441 cgccccgctc tctttagcaa aggtgttgtc ggcctgagcc actttcgccg gttcggctga
     4501 ggtttgatac gccacggtga cgttcaccgc ctgtgcctga aaagcgatga atgccagtgc
     4561 ggcaagaagt gtgttacgcg atgaaattgc catgattgtc tgctcccctg tcttgttatg
     4621 ggagcagtat tcaggaataa aaacattcat taaaagaatt agtcgttatc gcacagatga
     4681 ttttattctt agcaaaaaaa cggcaaagga aagatt
//
LOCUS       DAZSBF010000273         4701 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZSBF010000273 DAZSBF010000000
VERSION     DAZSBF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.278"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHXWD_003887"
     CDS             complement(<1..737)
                     /locus_tag="ACHXWD_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR7622651.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHXWD_003888"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHXWD_003888"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR7622652.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHXWD_003889"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHXWD_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR7622653.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHXWD_003890"
     CDS             4397..4678
                     /locus_tag="ACHXWD_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR7622654.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1129 a         1170 c         1182 g         1220 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg a
//
LOCUS       DAZSBF010000274         4698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZSBF010000274 DAZSBF010000000
VERSION     DAZSBF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.279"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..113
                     /locus_tag="ACHXWD_003891"
     CDS             <1..113
                     /locus_tag="ACHXWD_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR7622655.1"
                     /translation="GVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
     gene            151..612
                     /locus_tag="ACHXWD_003892"
                     /pseudo
     CDS             151..612
                     /locus_tag="ACHXWD_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1138..2313)
                     /gene="senB"
                     /locus_tag="ACHXWD_003893"
     CDS             complement(1138..2313)
                     /gene="senB"
                     /locus_tag="ACHXWD_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR7622656.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2382..4643)
                     /locus_tag="ACHXWD_003894"
     CDS             complement(2382..4643)
                     /locus_tag="ACHXWD_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR7622657.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYHSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1197 a         1100 c         1090 g         1311 t
ORIGIN      
        1 acggagtgga gccagaaagc tacctccgct atgtccttga cgtcatagcc gactggccga
       61 taaaccgggt cggcgaactg ctcccctggc gcgtagcact gccgactgaa taacacattc
      121 ccgtcaatac ggttcttgct gcacgcttac aaacaaaatg ggttggctaa agctctgaga
      181 gagattggcc gtatagaacg aacactgttt atgctggact ggtttcggga tccggcattg
      241 cggcgacgtg tccaggccgg gctgaacaaa ggagaagccc gtaatgctct ggcccgtgct
      301 gtattcatgc atcgattggg ggagattaga gatcggaaac cggaaaatca aagttatcgc
      361 gccagtggat tgactttatt gacggcggct atttccttat ggaacacagt ttacatggaa
      421 agagcggttg atgctctgaa gcgtaagggc atgaaaataa atgctcaact attatcgcat
      481 ttatcaccat taggttggga acatatcaat ctgacaggcg attatatctg gaaaaacagc
      541 cggataccga cttcaggtaa gtttcgtcgt ttgagatcag ttaagatcga caaggtcaaa
      601 aaacaacctt aacgtacaat aattttcgat atccaaactg accccaagta taaaaaacag
      661 cctgtcacgg tataaaaatc gaccatgcag aaattatgct ggttatttta ccgctgctgg
      721 tccctcattc agtgactgcc ccaggggttt tcatgctctc cttatagtac aataatttcc
      781 atatccaaac tcacccctac tttgactttt atcatagtca aaacctctta acgtgagttt
      841 tcgttccact gagcgtcagc cctctaaaac tactgaataa tcagttagaa aacaaacaat
      901 cggaaaaatc tggggttccg gcctagaacc ccatgaacag gcatagctgt gatttcattt
      961 aattattggc acaaatcagt tgatcgtaca atgctgtgcc tgtctcgttt tctggagatt
     1021 cctcagcctc atggagcggc gtaacaggcg taccggcagg acagagatgt ttgcttttcc
     1081 tagtccggcc agtttagtgg aataacggag tttttatccc atgttttttc cccccgatca
     1141 cgactttttc aaatcaatgc tcagaatacg ttctttagct ccttccacaa acacatcagg
     1201 atcccaatcg cggttaaagc cttctttgat gccaatgtaa agggtatcca tatcgtccga
     1261 caacaccatc tggtttgggt agaccggcat gttaaattca cgggtgatgc tgtatttggt
     1321 ggcatccact acgctgatgg tgccgctagt gaagttggtg acatacagtt cgttatattt
     1381 cggattgtat ttcaccgcgt tagctccggc tcctgtgtgc acatagccaa gctgtttacc
     1441 gtcgcgtaag tcaaaaatat agagtccatc ttttgtaccg gaacgatcgt catggtcgaa
     1501 taccaccaca aaggcacggt cagaagcgga gtccaccgcc agattctgca cacttgtgat
     1561 ttgtggtctc ggatctttca acgggatggt acgcagcagc gtctgatttt tcgcatcaat
     1621 ttcgttgatg taatctcttc cgccaacgta gattttctgt gttttttcat ccatcgtcag
     1681 accatacgct gtacctttgt aagcatcctt aacctcgcct tttaaggcaa gcgtcgtccc
     1741 gtcgagcatt aacagcttat gcagaggctt catgccctcg gccgttttca gcatattgct
     1801 ataactcaca aacagggtat cggaatcctg gctgtaaacc atatggcgaa aatgctcaaa
     1861 acgcgaatct gcagcgtttg ccgctttaaa acttaacctg tcgctggttc ttaccagttt
     1921 gccggttggg gtgtcaaaca tactgatacg cagggacgct gactgggtgt gtccgatata
     1981 gagctgatgt ttatcctgat ttatcgccag cgaaaatgcc cggtatggca tcgtgtattt
     2041 attttcgact ttcagcgaat ctgaattaaa cgaatacaga tagcctttat tggcttcacg
     2101 atttacacga tcggtgactg ctgcaaacac ctttttactg gcattatcaa acgccagttc
     2161 atagacgcct tcatcagggg actgaaggta attcgttttc agggcaaatc gcgaaagttc
     2221 gcgttcaggt ggatcgatag ccatggccat cgtgggtaaa aatagtgaaa ttaacaggta
     2281 tagcggtgct ttggataaag tgaaaatatt catttttcgt tcctgcaata aaattgacat
     2341 aagagagaaa acgtattgtc tgaacctagc ggaaattgtc atcagaaacg aatgtccccg
     2401 ccaaatatcc atgttctgcc acgcgcggaa ttggctgggt gggtctcgtc accaagacct
     2461 ggcatcatgt tgagcttatt cagcgcctcc gaataatctc tgttcatcag gttttgtacc
     2521 gaaagtttca gtgttaggtt gcggttgtac tcgtaagtac catagagatc gataatcgtt
     2581 gggatctgcg gcaaatcctg tttgattatt gcgccggtat gttcgtcctg ctcaaaatca
     2641 ggcgacaggc gacgagcctt gcctgtgtat tttatgatag tgcccagggt caacgcgtta
     2701 tcgaagaagc gaacaccagt atccagcgtc atgtattttc tgggcagttc ggtcatatcc
     2761 cctgcgccaa agtaggtgct ggcgatggag gttggctggt ctgtttgctg ctggctgaaa
     2821 gagagtcggc caaaagcaaa acctgcatca taatccatct ccagttcgaa gccctttgcg
     2881 ataacgtcgc ttgccgagtt aacgtagatg tacatattgt cgctgtattc atcgctaatg
     2941 ccctcccagt cattgccaat cacctcaggc atactgcatt tacgacctcc agaacaaacc
     3001 agataagact cgctgtagat gtagttctgg atcctgctgt ggtacgccag agccttaaag
     3061 cgcagggcat cctgttcgac cagtaaatct ctggtatcaa tgttaaaacc cgcctgccag
     3121 gtttctgcac gttcaggctt caggaatggg ttcatggatg cgcctcctga attagagaag
     3181 aacatctcct ggatgttcgg ggcgcgcatg gatttgctgt agccaatgaa cggctgaagc
     3241 catggtgtta cctgagcaga aagctgaact gaagggttga agccaccctc tttatcgtca
     3301 atatcgtagc taccctgtgg cacgcagata acgcgtgaat cgcaggcggg cttgtaccct
     3361 gtgatcctgt tacgtgtgta gttgagattg aaatctgcct cccagatgcc gcgcgtaacc
     3421 ttcagcccgg tatacagcgc tgaaatatcc tgctggccgg agggtgcgaa cggattgttc
     3481 tcgatcgatt cctgattcgc tttcgggtcg cccgccgctg agtgaatggt ccggtcatag
     3541 cgggtacgca tcagtttgct gcccagcata aactccaggt cattgtccgc gacagtgaac
     3601 cggctggtat tgttgatatc cagcgcgtca gaacgatttt gcgctgaggt tttgtagaaa
     3661 gtatacagcg agccatcacg atacttttga ttaccgcgac tggtactggc cgttacgtta
     3721 aagtcaatta attcagaaaa aggggtgtaa tgatatttaa tgtaatagtc gtcactggtt
     3781 atatcacgac gtgtaaattt attttcatag gttcgagcgg aaagttcgaa gctgttaaat
     3841 tcgtctggtc tgatatccat tttatacagt tgggatttgg ggttctgctt catatattta
     3901 tcataaccaa actctttgct gttaattcca gaaccatttg agaaattaga gtacacagaa
     3961 ctgccgctca cagcagccat aacgccaatg cttcccgtat ccgtaaaagc gtcgcttttt
     4021 ccaccaaggg cgatcattcc gctgcgtccc agcccattac taccgacaga gaaacgtgaa
     4081 cgaaggccat atgtattacc gttaaatatt acatcgtcaa cgccaatagt acgcatattt
     4141 gcactaccgg caagggcgtt gatcccttca gagccactgc tgtcaccgcg tgtaacatca
     4201 actgctacca gtaagttagg atctataagt acgccagcca tattgttagt tgaaccatgc
     4261 gtcgttgttc cggaggtaga ggttccgtaa aaactctggg taataccatc gaccatagtg
     4321 tttacacgac caaatccgct catgcctcga atattcacac tgattgctcc ctgaccagga
     4381 tctatttgag tataagtacc aggcatacta cgcactgttg cgtcaagtga ttgcaggttt
     4441 ttgtccgttg tccgggaact ggtcgcgcct gttttctcga gagcttcctg ctcactggtc
     4501 gtcttcccga cagaaacgct gagcggactg aattctgccg ctcctttttt gtcggaatta
     4561 gttttttcac tgatggattg tgagtaagca ccgcagctga gcaataatat gcctgagccg
     4621 atagccagtt ttataacgtt cataaatttc cttgggtata atattaataa agataagtat
     4681 aactatttgt ttattaat
//
LOCUS       DAZSBF010000275         4660 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZSBF010000275 DAZSBF010000000
VERSION     DAZSBF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.280"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            65..1105
                     /gene="pstS"
                     /locus_tag="ACHXWD_003895"
     CDS             65..1105
                     /gene="pstS"
                     /locus_tag="ACHXWD_003895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR7622658.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            1192..2151
                     /gene="pstC"
                     /locus_tag="ACHXWD_003896"
     CDS             1192..2151
                     /gene="pstC"
                     /locus_tag="ACHXWD_003896"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR7622659.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            2151..3041
                     /gene="pstA"
                     /locus_tag="ACHXWD_003897"
     CDS             2151..3041
                     /gene="pstA"
                     /locus_tag="ACHXWD_003897"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR7622660.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            3132..3905
                     /gene="pstB"
                     /locus_tag="ACHXWD_003898"
     CDS             3132..3905
                     /gene="pstB"
                     /locus_tag="ACHXWD_003898"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR7622661.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            3920..4645
                     /gene="phoU"
                     /locus_tag="ACHXWD_003899"
     CDS             3920..4645
                     /gene="phoU"
                     /locus_tag="ACHXWD_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR7622662.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1078 a         1176 c         1295 g         1111 t
ORIGIN      
        1 gtttgtccta ttttgcttct cgtagccaac aaacaatgct ttatgaatcc tcccaggaga
       61 cattatgaaa gttatgcgta ccaccgtcgc aactgttgtc gccgcgacct tatcgatgag
      121 cgctttctct gtgtttgcag aagcaagcct gacaggtgca ggtgcaacct tccctgcgcc
      181 ggtgtatgcc aaatgggctg acacttacca gaaagaaatc ggtaataaag ttaactacca
      241 gggtatcggt tcttccggtg gcgtaaaaca gattatcgct aataccgttg attttggtgc
      301 ctctgactcg ccgctgtctg acgaaaaact ggctcaggaa ggtctgttcc agttcccgac
      361 cgtgattggc ggcgtggtgc tggcggttaa cattccaggg ctgaagtctg gcgaactggt
      421 gctggatggt aaaaccctcg gcgacatcta cctgggcaaa atcaagaagt gggatgatga
      481 aaccatcgcc aaactgaatc cgggtctgaa actgccttca caaaacattg ctgtagtacg
      541 ccgcgcagat ggctccggga ctaccttcgt cttcaccagc tacctggcga aagtgaacga
      601 agagtggaaa aacaacgttg gtactggctc taccgtaaaa tggccgatcg gtctgggcgg
      661 taaaggtaac gacggtatcg ccgcgttcgt tcagcgtctg ccgggtgcaa ttggttatgt
      721 tgaatatgct tacgcgaagc agaacaacct ggcgtacacc aaactgatct ccgctgatgg
      781 taaaccggtt agtccgaccg aagaaaactt cgctaatgca gcaaaaggtg cagactggag
      841 caaaaccttc gctcaggatc tgaccaacca gaaaggcgaa gatgcatggc ctattacctc
      901 taccacgttc attctgatcc acaaagatca gaagaaacca gaacaaggca cagaagtgct
      961 gaaattcttc gactgggcgt acaaaaccgg ggctaaacag gcgaacgacc tggattacgc
     1021 cagcctgccg gatagtgtag ttgaacaggt tcgcgctgcg tggaagacca atattaaaga
     1081 cagtagcggt aagccgctgt actaataaaa ctccaggccg ggtacggtgt tttacgccgc
     1141 atccggcatt acaaaatgac tttgtaaacg cgtttaactg aagagtaact tatggctgca
     1201 accaagcctg cttttaaccc accgggtaaa aagggcgaca taattttcag cgtgctggta
     1261 aaactggcgg cgctgattgt gctattgatg ttgggtggca ttattgtctc tctgatcatc
     1321 tcctcctggc cgagcattca gaaatttggt ctggctttcc tatggaccaa agagtgggat
     1381 gcaccgaacg atatctacgg ggcgctggtg ccgatctacg gtacgttggt gacttcgttt
     1441 atcgcgctgc tgatcgccgt cccggtgagt ttcggtatcg ccctgttcct gactgagctt
     1501 gcgcctggct ggctgaaacg cccgctgggt atcgccattg agctgctggc agccattcca
     1561 agtatcgttt acggcatgtg gggcctgttt atctttgcgc cgctgttcgc cgtttacttt
     1621 caggagccgg tcggcaatat catgtcgaat atcccgattg ttggcgcgct gttctctggc
     1681 cccgcatttg gtatcggtat cctcgcggca ggcgtgatcc tcgccatcat gattattccg
     1741 tacattgcgg cggtaatgcg tgatgtgttc gaacaaaccc cggtgatgat gaaagagtcg
     1801 gcctacggta ttggctgcac cacctgggaa gttatctggc gtatcgttct tccgttcacc
     1861 aaaaatggtg ttatcggcgg catcatgctg gggctgggcc gcgcgctcgg tgaaaccatg
     1921 gcggtgacct ttatcatcgg taacacctac cagctcgaca gcgcctcgct gtacatgccg
     1981 ggcaatagta tcacctctgc gttggcgaac gaatttgcgg aagcggaatc cggtctgcac
     2041 gttgccgcac tgatggaact gggcctgatc ctgtttgtga ttaccttcat cgtcctcgcc
     2101 gcatcgaagt ttatgattat gcgcctggct aagaatgagg gggcacgcta atggctatgg
     2161 ttgaaatgca aaccactgcg gcgctggctg aatctcgccg caaaatgcag gcgcgtcgcc
     2221 gcctcaaaaa ccgtattgcg ctgacgctct cgatggcgac gatggccttc ggcctgttct
     2281 ggctgatctg gattttaatg tccaccatca ctcgcggtat cgacggtatg tcgctggcgc
     2341 tgttcactga aatgacgccg ccgcccaata cggaaggtgg tggtctggcg aacgccctgg
     2401 cgggtagcgg gctgttaatt ttgtgggcca cggtattcgg tacgccgctg ggcattatgg
     2461 cggggattta tctggcggaa tatggtcgta aatcctggct ggcagaagtg attcgcttca
     2521 ttaacgacat tctgctctcc gtgccgtcga ttgtggttgg tctgtttgtc tacaccatcg
     2581 tggtggcaca gatggagcac ttctccggct gggcgggcgt aattgcgctg gcgctgttgc
     2641 aggtgccgat cgttatccgc accaccgaga acatgctgaa actggtgccg gacagcctgc
     2701 gtgaagcggc ttatgcgctg ggtacaccga agtggaagat gatctccgcg attacgctga
     2761 aagcgtcggt gtccgggatt atgaccggta tcctgctggc gattgcccgt attgccggtg
     2821 aaaccgcgcc gctgctgttt accgcgctct ccaaccagtt ctggagcacg gacatgatgc
     2881 agccgatcgc caacctgccg gtgacgatct ttaagtttgc gatgagcccg tttgcggaat
     2941 ggcaacaact ggcctgggcc ggggtattga tcattaccct gtgcgtcctg ctgctgaaca
     3001 ttctggcgcg cgttgttttt gcgaagaata aacacggtta acactttgcc ggatgcggcg
     3061 tgaacgcctt acccggccta cggtaagcct gattagcgaa aagcatcagg tacgatgagg
     3121 aaaagattgc aatgagtatg gttgaaactg ccccgagtaa aattcaggtt cgtaatttga
     3181 acttctacta cggcaaattc catgccctga aaaacatcaa cctggatatc gctaaaaacc
     3241 aggtaacggc gtttatcggg ccgtccggct gcggtaaatc gacgctgctg cgtaccttca
     3301 acaaaatgtt tgaactgtat ccggagcagc gcgcagaagg tgaaattctg cttgatggcg
     3361 acaacatcct gaccaactct caggatatcg cactgctgcg tgcgaaagtg ggcatggtgt
     3421 tccagaaacc gacgccgttc ccgatgtcca tctacgacaa catcgctttt ggtgttcgtc
     3481 tgtttgagaa gctctcccgt gccgacatgg acgagcgcgt gcagtgggca ttgaccaaag
     3541 ccgcattgtg gaacgaaacc aaagataaat tgcaccagag cggttactct ctctctggtg
     3601 gtcagcaaca gcgtctgtgt attgcgcgtg gtatcgccat tcgcccggaa gtgctgctgc
     3661 tcgacgaacc gtgttcggcg ctcgacccca tctctaccgg gcgtattgaa gagctgatca
     3721 ccgaactgaa gcaggattac accgtggtga tcgttaccca caacatgcag caggctgcgc
     3781 gttgttccga ccacacggcg tttatgtacc tgggcgaatt gattgagttc agcaacacgg
     3841 acgatctgtt caccaagcca gcgaagaaac aaacagaaga ctacatcacc ggtcgttacg
     3901 gttaattcag gagtgcgtta tggacagtct caatcttaat aaacatattt ccggccagtt
     3961 caacgccgaa ctggaaagta tccgcacgca ggtgatgacc atgggtggca tggtggagca
     4021 gcagctttct gatgcaatca ccgcgatgca taaccaggac agcgatctgg cgaagcgcgt
     4081 gatcgaaggc gacaagaacg tcaacatgat ggaagtggcg atcgatgaag cctgcgtgcg
     4141 cattatcgcc aaacgtcagc cgacggcgag cgacctgcga ctggttatgg tgatcagtaa
     4201 aaccattgcc gagctggagc gtattggcga cgtggcggac aaaatctgcc gtactgcgct
     4261 ggagaaattc tcccagcagc atcagccgtt gctggtaagt ctggagtcgc tgggccgtca
     4321 taccatccag atgctgcacg acgtgctgga cgcgttcgcg cggatggaca ttgacgaagc
     4381 ggtacgtatt tatcgtgaag ataaaaaagt cgatcaggaa tacgaaggta ttgttcgtca
     4441 actgatgacc tacatgatgg aagattcgcg taccattccg agcgtactta ctgcgctgtt
     4501 ctgcgcgcgt tctatcgaac gtattggcga ccgctgccag aatatttgtg agtttatctt
     4561 ctactacgtg aaggggcagg atttccgtca tgtaggcggt gatgagctgg ataaactgct
     4621 ggcgggaaaa gatagcgaca aataattcac cagacaaatc
//
LOCUS       DAZSBF010000276         4621 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZSBF010000276 DAZSBF010000000
VERSION     DAZSBF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.281"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..743
                     /gene="gpmA"
                     /locus_tag="ACHXWD_003900"
     CDS             <1..743
                     /gene="gpmA"
                     /locus_tag="ACHXWD_003900"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR7622663.1"
                     /translation="KLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKLLKEE
                     GYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAETAEK
                     YGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDRVIPY
                     WNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEFDENF
                     KPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(902..1954)
                     /gene="aroG"
                     /locus_tag="ACHXWD_003901"
     CDS             complement(902..1954)
                     /gene="aroG"
                     /locus_tag="ACHXWD_003901"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR7622664.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            2270..2650
                     /locus_tag="ACHXWD_003902"
     CDS             2270..2650
                     /locus_tag="ACHXWD_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR7622665.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            2764..3705
                     /gene="zitB"
                     /locus_tag="ACHXWD_003903"
     CDS             2764..3705
                     /gene="zitB"
                     /locus_tag="ACHXWD_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR7622666.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3702..4421)
                     /gene="pnuC"
                     /locus_tag="ACHXWD_003904"
     CDS             complement(3702..4421)
                     /gene="pnuC"
                     /locus_tag="ACHXWD_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR7622667.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(4459..>4621)
                     /locus_tag="ACHXWD_003905"
     CDS             complement(4459..>4621)
                     /locus_tag="ACHXWD_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR7622668.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         1192 a         1187 c         1121 g         1121 t
ORIGIN      
        1 ctaagctggt tctggttcgt catggcgaaa gtcagtggaa caaagaaaac cgtttcaccg
       61 gttggtacga cgtggatctg tctgagaaag gcgtaagcga agcaaaagca gcaggtaagc
      121 tgctgaaaga ggaaggttac agctttgact ttgcttacac ttctgtgctg aaacgcgcta
      181 tccataccct gtggaatgtg ctggacgaac tggatcaggc atggctgccc gttgagaaat
      241 cctggaaact gaacgaacgt cactacggtg cgttgcaggg tctgaacaaa gcggaaaccg
      301 ccgaaaagta tggcgacgag caggtgaaac aatggcgtcg tggttttgcc gtgactccgc
      361 cggaactgac taaagatgat gagcgttatc cgggtcacga tccgcgttac gcgaaactga
      421 gcgagaaaga gctgccgctg actgaaagcc tggcgctgac cattgaccgc gtgatccctt
      481 actggaatga aactattcta ccgcgtatga agagcggtga gcgcgtgatc atcgctgcac
      541 acggtaactc tttacgtgcg ctggtgaaat atcttgataa catgagtgaa gaagagattc
      601 ttgagctgaa tatcccgacc ggcgtgccgc tggtgtatga gttcgacgag aatttcaaac
      661 cgctgaaacg ctattatctg ggtaatgctg acgagatcgc agcgaaagca gcggcggttg
      721 ctaaccaggg taaagcgaag taaacgtcat tcgtttaaaa atgagaaagc cgactgcaag
      781 tgagtcggct tttttgttgc taacaatgga gcacattgcc tgatgcgacg ctgcgcgtct
      841 tatcaggcct gtggtgattc atcggatacg ccactctgac ggcgcatccg acaattaaac
      901 cttacccgcg acgcgctttt actgcattcg ccagttgacg taacagagca tcggtatctt
      961 cccagccaat gcaggcatcg gtgatgctct taccgtaggc cagcggctcc ccgctatcga
     1021 ggctctgatt gccttccacc agatggcttt ccaccatcac gccaataatg gccttttcgc
     1081 caccggcaat ctgctggcaa acgtcagcac aaacatccat ctgctttttg aattgtttgg
     1141 acgagttagc atggctgaaa tcgatcatca cctgtgctgg caggcctgct ttgttcagcc
     1201 cttctttcac ttcagcaacg tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa
     1261 tgatatggca atcaccgtta ccgctggtat tcacaatcgc cgaatgcccc catttagtta
     1321 cggacaggaa gcagtgcggc gcaccggcgg cattaatggc atcgatagcc actttaatcg
     1381 taccgtctgt gccattttta aaacctaccg gacaagaaag accagacgcc agttcgcggt
     1441 gcacctgcga ttccgtggta cgtgcaccaa ttgcgcccca gctcatcagg tcagcgagat
     1501 actgaggagt gatcatatcg agaaactcac ccgccgctgg cagaccgctg tcgttaatat
     1561 caagcagcaa tttacgggct atacgcagac cgtcgttgat ctggaagctg ttatccatat
     1621 gcggatcgtt aatcaaccct ttccagccca ccgtggtacg cggcttttca aaatagacgc
     1681 gcattacgat ttccagctca tctttcagct cttcacgcag cgccagcaag cgagtggcat
     1741 actctttagc cgcgacagga tcatgaattg agcacgggcc aatcaccacc aacaggcgat
     1801 catcattacc tttcaggatc ttatggatcg cttttcgggc atgggcaacc gtattcgcgg
     1861 cattttcagt tacggggaat ttttccagca atgcgacagg aggaagtaac tctttgattt
     1921 ctttgatgcg taaatcgtcg ttctgataat tcatgtctgt tccagtgttg ccatacttat
     1981 cttagtgaat gcaatacttc caatctatat cttccgtcag aatgtgtaaa cggggtttta
     2041 cactatgaac ggattaatcc tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg
     2101 agatctctcc tacaatttct aactgtaact cctttgtttg ttaattattt caagattctc
     2161 tgctgcgttt cataaactgg ctgaaatctt aaaccaatgc cttatattca cctgcaaatg
     2221 cactgttgga agaggttatc cgacataacg accataacag gagcatccta tgaaaatgac
     2281 aaaactggcc acattatttc tgactgccac tctaagcctt gccagcggtg ccgcactggc
     2341 tgccgatagc ggagcgcaaa ctaataacgg ccaggcaaac gccgcagctg atgcgggcca
     2401 ggtagcccct gacgcccgtg aaaatgtcgc gccaaataac gtcgacaata acggggtaaa
     2461 taccggttct ggcggcacaa tgctgcattc ggatggttct tcaatgaaca atgacggaat
     2521 gaccaaagat gaagagcaca agaacaccat gtgcaaagat ggtcgatgtc cggatatcaa
     2581 taaaaaggta caaatcggtg atggcatcaa taatgatgtc gataccaaaa ccgacggtac
     2641 cacgcagtaa ccctgcaaaa tagccaataa tgggagagcg gagtctctcc cttttaattt
     2701 ttcacttcta aaagcagtat gatgtcataa agttaagtga gaataattaa gggatgactc
     2761 gtcatggcgc actcacactc acacacgtcc tcacacctgc cagaagataa taatgctcgt
     2821 cgcttgttgt atgctttcgg cgtgactgcc gggtttatgc tggtagaagt cgttggtggt
     2881 tttctttctg gttctctggc attgctggcc gatgcgggtc atatgttgac cgatactgcc
     2941 gccctgcttt tcgccctact cgccgtgcaa ttttcacgtc gtcctcccac cattcgccac
     3001 actttcggtt ggctaagact gaccacgctc gcggcgtttg tgaatgccat cgccctcgtg
     3061 gtaattacca ttttgattgt ctgggaggcg atagaacgct tccgcacgcc gcgtccggtc
     3121 gagggcggca tgatgatggc aattgccgtg gctgggttgc tggcaaatat actttctttc
     3181 tggttacttc atcacggcag cgaagagaaa aacctcaacg tacgagcagc ggcattgcat
     3241 gtgctcgggg acctgttggg ttcggttggg gctattattg ccgcgttgat tattatctgg
     3301 accggctgga cacctgctga ccccattctc tcgatagtgg tgtcgcttct ggttctgcgt
     3361 agcgcgtggc gactgttgaa agatagcgtg aatgaattac ttgaaggtgc accggtatcg
     3421 ctggatatcg ctgaactgaa gcgtcgtatg tgccgggaaa tcccggaagt ccgcaatgtg
     3481 caccatgtac atgtatggat ggtaggcgag aagccggtga taacgctgca tgtgcaggtg
     3541 atcccgccac acgatcacga tgccttgttg gatcagatcc aacactacct gatggatcac
     3601 tatcagattg agcacgccac cattcagatg gaatatcaac cttgtcatgg gccagactgc
     3661 cacctgaatg aaggggtgtc tggccattca catcatcacc attaatggga cagcgcgcgt
     3721 gagcctcttt cacgtgcgct gttgatccac atccgactgc cgttgagcgc aataaaggtc
     3781 aggatgatgt actccagaga catggcgtaa acgccctgaa gtgcaaaaat aacgacgcta
     3841 atcacgttaa taatcaccca caacagccag ttttccacat acttacgcgt catcagaatc
     3901 attgccacga tagataacac catcatgcat gaatcccaga acgggaaagc gtccggttgc
     3961 agttcaggca tcacaacctg taatcctaat gcttgcatga tcatgactgc cacgcgggtc
     4021 aaaaatgcaa acaccggatt gataaatacc gtcatcagac caatcgaaac aacgcaaacc
     4081 gccaaccagc tgagcgcctt cggcaatggt aaccagcgaa ttttcaactc cgcctcgttc
     4141 tgactggttt gtcgcgacca cgcataccaa ccgtaaatat tcgcggcaaa gaaaaacacc
     4201 tgtaatagca ggctggcata cagctgaatc tgaaagaaaa taatgccaaa caaggtgacg
     4261 ttgatcaggc caaagaagta gttgctgatc ttctccagac tggcaaggcc aatacacagc
     4321 aacccggcga tcgtgcctac cgcttcgatc catgagagat cataaccgcc tgcccctatt
     4381 ggtatatgta ccaggatatt ctgcacacta aaaaaatcca tctttccccc aaagcgtaac
     4441 gttacgcctt attattcgtt atccacgtag tgtagccgca aaatccagca tacgattgag
     4501 cggcaccagc gccctctctc gcagcctttc atcaagaacc tcgtgattgc ttccttcctg
     4561 ttctaatgcc tctgcgatgg actgaaggcc attcatggcc atccacggac aatgcgcgca
     4621 g
//
LOCUS       DAZSBF010000277         4606 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZSBF010000277 DAZSBF010000000
VERSION     DAZSBF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.282"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..2162
                     /locus_tag="ACHXWD_003906"
     CDS             <1..2162
                     /locus_tag="ACHXWD_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR7622669.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2396..3679)
                     /locus_tag="ACHXWD_003907"
     CDS             complement(2396..3679)
                     /locus_tag="ACHXWD_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR7622670.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3814..>4606)
                     /locus_tag="ACHXWD_003908"
     CDS             complement(3814..>4606)
                     /locus_tag="ACHXWD_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7622671.1"
                     /translation="QKTLINYMSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKA
                     ASAKLIRSTLSDAFREAIAEGHITTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESS
                     PCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGI
                     SMKETLDKCKEILGGETIIASTRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELR
                     SLSARLYEKQISDKFAQHLLGHKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1093 a         1142 c         1194 g         1177 t
ORIGIN      
        1 aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat atggataaac
       61 ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt attgtacaga
      121 ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc aactgccata
      181 actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt ggtttatcgg
      241 cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc catcaatata
      301 tgcgtgagat gatggcaggc gggggcaaaa tgatcctcgg gtcagacagc cacacccgtt
      361 acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta aaacagctgc
      421 ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg accggaaaac
      481 ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg gtgttcaaaa
      541 acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc gcgctctcta
      601 ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta agctccgtct
      661 ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggc caggattact
      721 gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt gatttaagcg
      781 ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa atcgacacac
      841 tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa cgcgtggcgc
      901 acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg aaagtgcagc
      961 aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg gcggcgaatg
     1021 cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcagtttac ccgtcatcac
     1081 agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt ggcgcaggcg
     1141 caatcatcag aaccgcgttc tgcggcccat gctttggcgc gggcgatacg ccaatcaaca
     1201 acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc tctaagccag
     1261 ctaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct gcgactgcgg
     1321 caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac gtgccggagt
     1381 acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg aaaggggcaa
     1441 ctcagcaacc gctgatttac gggccgaaca ttaaagactg gccggaattg ggtgcgctga
     1501 ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtgacc accaccgacg
     1561 aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt ctggcggagt
     1621 ttaccctgtc tcgccgtgat cccggttatg ttggcagaag taaagcgact gctgagctgg
     1681 aaaatcagcg tctggcgggg aatgtcagcg agctgacaga ggtgtttgcg cgcattaagc
     1741 agattgctgg tcaggagcat attgatccgc tgcaaactga aattggcagc atggtctatg
     1801 cggtgaaacc aggcgatggt tctgcgcgtg aacaggcggc gagctgccag cgtgtgattg
     1861 gcggtctggc gaatattgcc gaggagtacg cgactaaacg ctaccgttct aacgtcatca
     1921 actgggggat gttacctctg cagatggcgg aagtgccaac ctttgaagtg ggggattaca
     1981 tttacatccc tggcattaaa gcggcgctgg ataatccggg tacgacgttt aaaggttatg
     2041 tgatccatga agatgcgccg gtaacggaaa ttacgctcta tatggaaagt ctgactgctg
     2101 aagagcgcga gattatcaag gcgggtagtt tgattaactt caataaaaac cgtcagatgt
     2161 aaaaagcgcc atgtgaatgt aggtcgcatt cggcacttat tgtcggatgc gatgcttgcg
     2221 catcttatcc gacctacgaa tcgcatcgaa tctgtaggcc agataaggca tttacgcagc
     2281 atccggcact tattgtcgga tgcgatgctt gcgcatctta tccgacctac aaatcgcatc
     2341 gaaccgtagg ccggataagg cgtttacgcc gcatccggca aatagttaat tgctcttact
     2401 tcttcgcctc tgcaaccact ttactaccca cgccgcggtt attgtattcc cacatgcggt
     2461 tgtagttagt gtcattcaga ttgcgctgta tttcgtcgtt atcatctacg ctgccggtat
     2521 tacccgcaaa cggacgatta gagatcaccg catcggccca cggtttagcc gtgttaaaac
     2581 cttcgttgat ggcgctatca cggatcacca cctgaccgtt ggtattggca tcaacatcca
     2641 gcgagcggcc cagttgcgcc acaccatcac cgaaagcatt gaaacggctg tttacggcga
     2701 ggaaaccgta gtacatgttg gacagcgtag ccggtgcaaa cacatacgct tcttgctgag
     2761 tacgtgagtt caccacgcgg aattcggtgt tatcgaacac cactgcgccg cgaccagaaa
     2821 cgatatccac atccccttca atgtagctgt tggtcaccag cgtacgcggc tgacgattcg
     2881 tttccagacg gttctgcaca ccgctgttgg tgacaaagaa ggtgttctga cgaccgagaa
     2941 tgttaacgtt gttaatctgt acctggtcac catcagtacg cagtgccacc gccggatggt
     3001 tacctgcatc tacgctatcg cccagcgtgt tttcgatggt cagattttgc agttgcaggc
     3061 cattgttttg tgaccagaag accgcagagc agagaacacc gatactgtcg ctgcgtttgc
     3121 tctggcagct atcgtacata taccacgctg gtttacctgg catatatttg ccgcgcgggt
     3181 tgacgtcgtg acgccagtcg gcagggctca tgccaccatc aagggaaagc ccaatcttca
     3241 catcaatcgg tttttcacct gtaccgtaca gagtaattcc acccggagcg gcagggacat
     3301 ataccgttcc ctgatactca ccaggcatca cggcaatata ctggcgcttg ttggtacgct
     3361 tgataattgc cgcatctacc gccgcctgaa tcgtggtatg cgttacacct tgagtgcccg
     3421 ccgggccgac aacaaagtca ggttgcgcag gcagggtaat cggggaagga ttccacgctg
     3481 cagcacctgg tgtcagggat gcaaaatagt gttgagcatc gaaattctgc gcttcttttg
     3541 ccgacagaat cgggcgagaa gaggtaccag gcgcggtttg atcagaagga cgttgatcgg
     3601 gcggggttga gctacaggcg gtcagcgtca cgccaaaagc caatgccagc gccagacggg
     3661 aaactgaaaa tgtgttcaca ggttgctccg ggctatgaaa tagaaaaatg aatccgttga
     3721 agcctgcttt tttatactaa gttggcatta taaaaaagca tcgcttatca atttgttgca
     3781 acgaacaggt cattatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc
     3841 cctgcctctg tcatcacgat actgtgatgc catggtgtcc gacttgtgcc cgagaagatg
     3901 ttgagcaaac ttatcgctta tctgcttctc atagagtctt gcagacaaac tgcgcaactc
     3961 gtgaaaggta ggtggatccc cttcgaagga aagacctgat gcttttcgtg cgcgcataaa
     4021 ataccttgat actgtgccgg atgaaagcgg ttcacgacga gtagatgcaa ttatggtttc
     4081 tccgccaaga atctctttgc atttatcaag tgtttccttc attgatatcc cgagagcatc
     4141 aacatgcaat gttgtaggga tggcaatttt tacgcctgtt ttgctttgct cgacataaag
     4201 atatccatct acgatatcag accacttcat ttcgcataaa tcaccaactc gttgcccggt
     4261 aacaacagcc agttccattg caagtctgag ccaacatggt gatgattctg ctgtttgata
     4321 aattttcagg tattcgtcag ccgtaagtct tgatctcctt acctctgatt ttgctgcgcg
     4381 agtggcagcg accggttttg ttgttatatg gccttcagct attgcctctc ggaatgcatc
     4441 gctcagtgtt gatctgatta acttggctga tgccgccttg ccctcgtcta tgtatccatt
     4501 gagcattgcc gcaatttctt ttgtggtgat gtcttcaagt ggagcatcag gcagccccct
     4561 ccttattgct ttaattttgc tcatgtaatt tatgagtgtc ttctgc
//
LOCUS       DAZSBF010000278         4482 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZSBF010000278 DAZSBF010000000
VERSION     DAZSBF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.283"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHXWD_003909"
     CDS             complement(7..1068)
                     /locus_tag="ACHXWD_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR7622672.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHXWD_003910"
     CDS             complement(1065..2462)
                     /locus_tag="ACHXWD_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR7622673.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHXWD_003911"
     CDS             2617..3615
                     /locus_tag="ACHXWD_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7622674.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHXWD_003912"
     CDS             complement(3726..>4482)
                     /gene="ompG"
                     /locus_tag="ACHXWD_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR7622675.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1229 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg cc
//
LOCUS       DAZSBF010000279         4409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZSBF010000279 DAZSBF010000000
VERSION     DAZSBF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.284"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHXWD_003913"
     CDS             complement(<1..105)
                     /locus_tag="ACHXWD_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622676.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHXWD_003914"
     CDS             complement(173..2122)
                     /locus_tag="ACHXWD_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR7622677.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHXWD_003915"
     CDS             complement(2207..2542)
                     /locus_tag="ACHXWD_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR7622678.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHXWD_003916"
     CDS             complement(2542..2898)
                     /locus_tag="ACHXWD_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR7622679.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHXWD_003917"
     CDS             complement(2898..4394)
                     /locus_tag="ACHXWD_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR7622680.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1314 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZSBF010000280         4409 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZSBF010000280 DAZSBF010000000
VERSION     DAZSBF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.285"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(21..1967)
                     /locus_tag="ACHXWD_003918"
     CDS             complement(21..1967)
                     /locus_tag="ACHXWD_003918"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR7622681.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SADMTFRVKPESLILTIFFLGLIP"
     gene            complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHXWD_003919"
     CDS             complement(2133..2216)
                     /gene="yqgH"
                     /locus_tag="ACHXWD_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR7622682.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2369..3076
                     /locus_tag="ACHXWD_003920"
     CDS             2369..3076
                     /locus_tag="ACHXWD_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR7622683.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="ACHXWD_003921"
     tRNA            3182..3257
                     /locus_tag="ACHXWD_003921"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="ACHXWD_003922"
     CDS             3455..>4409
                     /locus_tag="ACHXWD_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7622684.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1162 c         1057 g         1041 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta cttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZSBF010000281         4407 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZSBF010000281 DAZSBF010000000
VERSION     DAZSBF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.286"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHXWD_003923"
     CDS             complement(<1..590)
                     /locus_tag="ACHXWD_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR7622685.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHXWD_003924"
     CDS             complement(583..993)
                     /locus_tag="ACHXWD_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR7622686.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHXWD_003925"
     CDS             complement(999..1532)
                     /locus_tag="ACHXWD_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR7622687.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2598)
                     /locus_tag="ACHXWD_003926"
     CDS             complement(1510..2598)
                     /locus_tag="ACHXWD_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR7622688.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLVLGENILSCDTERSIRERFSEYQVSGQRPG
                     NDDDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRE
                     TTYTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGP
                     ADAYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2595..3986)
                     /locus_tag="ACHXWD_003927"
     CDS             complement(2595..3986)
                     /locus_tag="ACHXWD_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR7622689.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4033..>4407)
                     /locus_tag="ACHXWD_003928"
     CDS             complement(4033..>4407)
                     /locus_tag="ACHXWD_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622690.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1056 a         1286 c         1041 g         1024 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcaccagc gccgtcgccg ctttacctgt tccgggtttt cccagaacca gtcgcccgca
     2101 ttcatcgtca taagccagcg tctgaacctg ccccagtaac cggttaagac aatcggcaac
     2161 ggtttcgccg tgttccggct gggcatcaat caccgctgtc tgcggcacgc cagcatcaac
     2221 aacggtgatg ccaaatggcg cagccagttc actgactatt ctgagcaggt tttttccgct
     2281 ctgctggagt ggcaaagcag agcagtcaac cagatcggct gttttgcttc gcccgacaat
     2341 tcccatgctg acgctgctgg cgtcataacg aagcggtagt gcctccacat atccggtgag
     2401 cacaggctca tccccgataa gcacttcaac cagctcacca ttttttatcc ggggttgata
     2461 atcccggctt ccgggccagc gggtggtaat ggcaacatta aaatcccggg caatacggtt
     2521 aatgcccgca ctgatacgga cggatgtcca gccgccccat tcgcgaccgg aaacccgaag
     2581 taaaacggta tcattcatct gacgggtacc cttaatgccc tgaccggaac aaagcccgga
     2641 tgagagatgg catttcgatc caggatatca gtttcacggg acgcatcgtc gtaccatgac
     2701 gcagccagaa caagcgcagg cagaacttcc gctggcgttc gctctgcggt ttcctccgtc
     2761 tgaaccagac gcgcctgaat atcccggttc agttctgtac gtaatgacgt cagctgaaaa
     2821 aacagcctgt catccgccgt acgcctgagc tcctgttcaa tcgccgcgtt cagcgatttg
     2881 cgtatgatgg tgagattttc ccgcgtgggt ggttgcgctc tctcatcctg tcctgtgctg
     2941 gctgccacgc tgtcaagtgc cggatgagaa acgtgaataa tatcggactg acgttcagtg
     3001 gaaccaccaa cagccacaac cgcctgctga tttttcacca ggcttccggg ttgtggcagt
     3061 gaagtgaccg cccttgtggc ttcgctgatt gccgtcgtcc ggatgacagc agccacaagg
     3121 tttgtctgct gtttttgcct gataaccgat gcggaatctg tgggccacac cgcacgcggt
     3181 gccagtcctg ggtccagcgt aatgccggac attgtcgtta tggactgcac cagatcctgt
     3241 gtgttatcca ccagccgggt tccggcccgc caggtatcct gcaacgtatg cacaaaatca
     3301 ctggcaaccg acggcggcat cagaatgacg gataaatcgc cctgcagtag tctcgctccg
     3361 gcagaaacag ccgaattaac cattttgaaa gctgtctgaa cggttcccag catatcggtc
     3421 gtccgggcaa taacatcgtt ctgaataaag tccggtattc ccgcgagatc aaaatcgccg
     3481 aacatgtctt caatcaactc atccaggaat acggacgact cctccagttt tctggcggtt
     3541 gcagctccgg cgaccggaaa tgacagttcc ccgctctcaa caaactgaaa tgaaacccgg
     3601 cacatacggc cttcagtgcc ggagtgagaa acggtcacct gcccgtcaat acagccctgc
     3661 atttcaccga actgcggatg gatcagcgtc cccggcccgg cggtttcaat cgctgtaatc
     3721 agcctgtcgc gctgctctgc gtaatcatca ccaacgagat acgcattaat cgtcagccgt
     3781 cgcgtggcac gcccgagatc ttccgtgtac ggtttgtcac ggtttgggta ttcatggacc
     3841 tgaacgcggc gtccgaacga accctcgtcg ctctccaccg aaaacggaac gccacgaaat
     3901 gaagcgtcgt aaagattatc gcgccaattg atacctgaag atgcagaaaa gagtgaagaa
     3961 agagaaggta aagaaggtaa gtccatactg tctccactat aaaaaaaccg ccaatttctg
     4021 gcggcttata tattaatcat cattaatatc tttcatattc aatttgcaat tttcgtaggt
     4081 cactcccgac tcgccattaa cattaactcc agctctgata ttcccgtttt cttttacatc
     4141 tacaaaaaac tggcctcttg gcatcttgaa cgcaaatgta aaaccaacag tatctgaatc
     4201 aaaatcatca tctttatata ttgggatcaa aaatgtcatc gtcgctggtg atgcattgtt
     4261 tgcaaaatca aatacaccta tacttttgcc gaattcataa gaagcttttc tcaatataat
     4321 cgcaccgctg gcagaagggg atgggcacga tatcctcagt tgtctgaatc catctgtatc
     4381 caagtcaacg tcattatctg cagatgt
//
LOCUS       DAZSBF010000282         4404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZSBF010000282 DAZSBF010000000
VERSION     DAZSBF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.287"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(139..3654)
                     /locus_tag="ACHXWD_003929"
     CDS             complement(139..3654)
                     /locus_tag="ACHXWD_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR7622691.1"
                     /translation="MDKNALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..>4404)
                     /locus_tag="ACHXWD_003930"
     CDS             complement(4106..>4404)
                     /locus_tag="ACHXWD_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR7622692.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1141 a         1127 c          905 g         1231 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggt aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acag
//
LOCUS       DAZSBF010000283         4400 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZSBF010000283 DAZSBF010000000
VERSION     DAZSBF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.288"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            2..698
                     /locus_tag="ACHXWD_003931"
                     /pseudo
     CDS             2..698
                     /locus_tag="ACHXWD_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1451
                     /locus_tag="ACHXWD_003932"
     CDS             705..1451
                     /locus_tag="ACHXWD_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR7622693.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1466..1888
                     /locus_tag="ACHXWD_003933"
     CDS             1466..1888
                     /locus_tag="ACHXWD_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7622694.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1946..2302
                     /locus_tag="ACHXWD_003934"
     CDS             1946..2302
                     /locus_tag="ACHXWD_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622695.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2471..3136
                     /locus_tag="ACHXWD_003935"
     CDS             2471..3136
                     /locus_tag="ACHXWD_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR7622696.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3364..3519
                     /gene="hokD"
                     /locus_tag="ACHXWD_003936"
     CDS             3364..3519
                     /gene="hokD"
                     /locus_tag="ACHXWD_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR7622697.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            3769..4056
                     /locus_tag="ACHXWD_003937"
     CDS             3769..4056
                     /locus_tag="ACHXWD_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622698.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            4095..>4400
                     /locus_tag="ACHXWD_003938"
     CDS             4095..>4400
                     /locus_tag="ACHXWD_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR7622699.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     "
BASE COUNT         1195 a          972 c         1212 g         1021 t
ORIGIN      
        1 tattcgcttc tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc
       61 tcgcggcgtt acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc
      121 gttaattcag gttggatggc tggtcgaaaa tgacggaggg ctttctctac ctaactttga
      181 acgtcataac ggaaaaagct ctaaaaaaaa cgggcggtta caaacgagcg agtaacaaaa
      241 atacgcgaac tgaaacgaaa aggtaacgct gccagcgtta cacaaacgga tcaaaaagcg
      301 ttaccagagg aagaggaaga ggaagaggaa gaggaagagg aagaggaaga tctaaatact
      361 gatctccccc tcgccaaaaa cgagcgtcta aaaaattcga gccggaggct attgagctgc
      421 ccgattggtt gccggaaaca ctctggcatg agtgggtccg gttcagacag gcattgcgaa
      481 aaccgattcg aacggagcag ggcgctaacg gggcgatacg ggaactggaa aaattccgtc
      541 agcagggttt tacacctgag caggtgattc gacacagcat cgccaatgaa taccagggcc
      601 tgttcgcgcc gaaaggtgtt cggcctgaga cgttgctccg acaggttaac accgtctcgt
      661 tgccggacag tgcgatcccg ccaggcttca gggggtaacg gaccatgaaa aatattgcga
      721 caggcggcgt tctggaacgc atccgccgac tgaccccgcc acatgtaacc gccccattca
      781 gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt agcgaggaga
      841 tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct gaatcgctca ggcatccagc
      901 cgttgcaccg taaatgctcg tttgcgaatt accaggtgca gaacgacggc cagcgatacg
      961 cgttaagcca ggcgaaatcc atcgccgatg aactgatgac cgggtgtaca aattttgcgt
     1021 tcagcggaaa acctggtacc gggaagaatc acttagcggc agctatcggg aatcgcctgc
     1081 tgaaagacgg tcagacagtg attgtggtta ccgtggctga tgttatgagc gccctgcacg
     1141 ccagctatga cgacgggcag tcaggcgaaa aatttttgcg ggagctgtgc gaagtggatc
     1201 tgctggttct tgatgaaatt ggcattcagc gcgagacgaa aaacgagcag gtggtgctgc
     1261 accagattgt tgatcgccgg acagcgtcga tgcgcagcgt ggggatgctg acaaacctga
     1321 actatgaggt gatgaaaaca ttgctcggcg agcgggtgat ggatcgcatg gtcatgaacg
     1381 gcgggcgctg ggtgaatttt aactgggaga gctggcgtcc gaatgttagc cattcgaggg
     1441 ttgttaagta gtttcaggag gatttatggc gaaacctttt actcccgaac agcgggaaga
     1501 actgaagacg cgaattgtgg aactcgtgca tcaggacggt cgggtcacga ttcggcagtt
     1561 gtcagatgaa acaggtatca gtcgtgcgtc tgtcggtcgc ttatgcatag aactggtcgc
     1621 aagtggtgat gtatataatt ctggctacgg cttattcccg tctgaacagg ctcgcaagga
     1681 ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg aggaaaccgg ttgttgttga
     1741 tccggacctt atctggtcat tacctgacgg agaaatacgc cgctacgaca ggcgcctgaa
     1801 tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
     1861 gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc aattaccgct
     1921 ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt taagcaatat
     1981 cgttttattt ccggtaaaag aggatgaccc tcgtaatcag gttaattttc tttatgagcc
     2041 atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag agcgtcaggt
     2101 ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct ctgtggcaaa
     2161 aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac aggaaaggcg
     2221 gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga tacgcagggc
     2281 gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat ccttaaccgg
     2341 agggatttct gcaccctcaa atcatcagga gaccacccga aagggcgggg agcagtcaca
     2401 catctgtttc cgatagcccc gttctaatgc tacactcttt gatattttta tgaccccaat
     2461 aaacatattt atgacagttg ctgatttcaa acggcccaaa ttggagctcc caaacggggc
     2521 aaacaaacta ctactgcact cttgctgtgc tccatgttcc ggtgaggtga tggaggcgct
     2581 tcaggcctcg ggaatcgact acaccatctt tttctacaac ccgaacattc atcctcagaa
     2641 agagtattta attcgtaagg atgagaatat tcgctttgct gaacaacacg gcgtgccgtt
     2701 tattgatgct gattacgaca cagacaactg gtttgaacga gccaaaggaa tggaatggga
     2761 gcccgaacga gggatccgtt gcaccatgtg ttttgacatg cgttttgagc ggacagcgct
     2821 gtacgccgct gaaaatggtt tcagtgtgat cagcagttca ctgggcattt cacgctggaa
     2881 aaatatgcag caggttaacg actgtgggcg gcgagccgtc gcgcattatc cgggcatggt
     2941 gtactgggat tataactggc gcaagcaggg cggctcgtcc cgtatgattg aaatcagcaa
     3001 gcgcgaaaaa ttctatcagc aggaatattg tggctgtgtg tattctctgc gcgataccaa
     3061 tctacaccgc aaatctcagg gacgccctct tatcaaaatt ggtcaactcc actacggtaa
     3121 agaagagaag gagtgatttt atggggcacc tttctgattg atttcatatt ggcgaggtaa
     3181 gtagaatgac tgcgggtgct tgaggctatc tgcttcaggc atgaacacca aaaggcagat
     3241 agagaaaagc cccagttaac attacgcgtc cggcaagacg cttaacatta atctgaggcc
     3301 aatttcatgc tttgcacatg taggttagcc tcttacatgc cgaaaggcaa ggagaagcag
     3361 gctatgaagc agcaaaaggc gatgttaatc gccctgatcg tcatctgttt aaccgtcata
     3421 gtgacggcac tggtaacgag gaaagacctc tgtgaggtac gaatccgaac cggccagacg
     3481 gaggtcgctg tcttcgtaga ctacgaatcc aggaagtaag agtgaccggg cggggagctg
     3541 atcccatccc cgcccacctc tgatgtgtca ggcatcctca acgcacccgc acttaacccg
     3601 cccatcgctg tgatctctca gcgtttcggc gggttttttg ttgtttattt ccggtgaatt
     3661 tgattcgcgc accttcgcag atagaatcga ctcacttaag tagcgcgcag ggagaagagg
     3721 gatggacccc gaacagggga gagctattta tctggaagga ttctgaagat gaaaatcgaa
     3781 gaattgcgtg aaatttttag tgaaaatggc ctctatgctg tgcgcgttga gaatggagcg
     3841 attgtcagcc attgccgcat tagatgtttg caatctcaac aaaggaagag cggtgctgtg
     3901 ttattttatt tttgtaatgg acttctgacg gacggtttta ttttgcgtga ggacgaattt
     3961 gtcacatcat tacgggtttt gaaagagatt ggttttaagg ctggttttct gcttttgctg
     4021 aagaataaac tcatctacaa tcttgagcag gattgaactc ctgctgtgta acaccgtgcc
     4081 accggagaaa gccgatggca catatacaac tggtcaaaca aacctcttcc ggattacttc
     4141 tcccggcgac gccggagagt tgcgattttc tgcatcaaat caaaataggt gagtggatac
     4201 acgcagactt taagcgtgtg tgtaactacg cattccacaa gcgttttttc aaactcctgc
     4261 aactgggttt cgattactgg actccggtcg gtggggcgat cacgcctcgc gaacgaaaac
     4321 tggtgtccgg tttcgttgat tacctgtgtg aatcagtagg ccgggaacat acgccagctc
     4381 tgagcgaagc cgcagagcaa
//
LOCUS       DAZSBF010000284         4358 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZSBF010000284 DAZSBF010000000
VERSION     DAZSBF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.289"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1515
                     /gene="ipaB"
                     /locus_tag="ACHXWD_003939"
     CDS             <1..1515
                     /gene="ipaB"
                     /locus_tag="ACHXWD_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFR7622700.1"
                     /translation="SQLTLLIGNLIQILGEKSLTALTNKITAWKSQQQARQQKNLEFS
                     DKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQIQTRLSELDPDSPEKKKL
                     SREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLEKEIDSFSAFSNTASAEQL
                     STQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQSLQESRKTEMERKSDEYA
                     AEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASLALAAVGLALMVTDAIVQA
                     ATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVDSKKAKMIGSILGAIAGAL
                     VLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFLKNFSSQLDDLITNAVARL
                     NKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGSVASAVFQNSASTNLADLT
                     LSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQANRTDVAKAILQQTTA"
     gene            1478..2626
                     /gene="ipaC"
                     /locus_tag="ACHXWD_003940"
     CDS             1478..2626
                     /gene="ipaC"
                     /locus_tag="ACHXWD_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFR7622701.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKSTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSLSSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSPQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMDTSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSITQSRNSTASQIAGNIRA"
     gene            2677..3675
                     /gene="ipaD"
                     /locus_tag="ACHXWD_003941"
     CDS             2677..3675
                     /gene="ipaD"
                     /locus_tag="ACHXWD_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFR7622702.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKKKYEDKPLYPATNTV
                     SQKEADKWLTELGGTIGKVSKKNGGYVVNINMTPIDNMLKSLNNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            3684..>4358
                     /gene="ipaA"
                     /locus_tag="ACHXWD_003942"
     CDS             3684..>4358
                     /gene="ipaA"
                     /locus_tag="ACHXWD_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR7622703.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKESFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQR"
BASE COUNT         1600 a          875 c          746 g         1137 t
ORIGIN      
        1 tcccaactaa cgcttttaat tggaaacctt attcaaatac tcggtgaaaa atctttaact
       61 gcattaacaa ataaaattac tgcttggaag tctcagcaac aggcaagaca gcaaaaaaac
      121 ctagaattct ccgataaaat taatactctt ctatctgaaa ctgaaggact aaccagagac
      181 tatgaaaaac aaattaataa actaaaaaac gcagattcta aaataaaaga cctagaaaat
      241 aaaattaacc aaattcaaac aagattatcc gaactcgacc cagattcacc agaaaagaaa
      301 aaattaagcc gggaagaaat acaactcact atcaaaaaag acgcagcagt taaagacagg
      361 acattgattg agcagaaaac cctgtcaatt catagcaaac ttacagataa atcaatgcaa
      421 ctcgaaaaag aaatagactc tttttctgca ttttcaaaca cagcatctgc tgaacagcta
      481 tcaacccagc agaaatcatt aaccggactt gccagtgtta ctcaattgat ggcaaccttt
      541 attcaactag ttggaaaaaa taatgaagaa tctttaaaaa atgatctggc tctattccag
      601 tctctccaag aatcaagaaa aactgaaatg gagagaaaat ctgatgagta tgctgctgaa
      661 gtacgtaaag cagaagaact caacagagta atgggttgtg ttgggaaaat acttggggca
      721 cttttaacta tcgttagtgt tgttgcagca gctttttctg gaggagcctc tctagcactg
      781 gcagctgttg gtttagctct tatggttacg gatgctatag tacaagcagc gaccggcaat
      841 tccttcatgg aacaagccct gaatccgatc atgaaagcag tcattgaacc cttaatcaaa
      901 ctcctttcag atgcatttac aaaaatgctc gaaggcttgg gcgtcgactc gaaaaaagcc
      961 aaaatgattg gctctattct gggggcaatc gcaggcgctc ttgtcctggt tgcagcagta
     1021 gttctcgtag ccactgttgg taaacaggca gcagcaaaac ttgcagagaa tattggcaaa
     1081 ataataggta aaaccctcac agaccttata ccaaagtttc tcaagaattt ttcttctcaa
     1141 ctggacgatt taatcactaa tgctgttgcc agattaaata aatttcttgg tgcagcgggt
     1201 gatgaagtaa tatccaaaca aattatttcc acccatttaa accaagcagt tttattagga
     1261 gaaagtgtta actctgccac acaagcggga ggaagtgtcg cttctgctgt tttccagaac
     1321 agcgcgtcta caaatctagc agacctgaca ttatcgaaat atcaagttga acaactgtca
     1381 aaatatatca gtgaagcaat agaaaaattc ggccaattgc aggaagtaat tgcagatcta
     1441 ttagcctcaa tgtccaactc tcaggctaat agaactgatg ttgcaaaagc aattttgcaa
     1501 caaactactg cttgatacaa ataaggagaa tgttatggaa attcaaaaca caaaatcaac
     1561 ccagatttta tatacagata tatccacaaa acaaactcaa agttcttccg aaacacaaaa
     1621 atcacaaaat tatcagcaga ttgcagcgca tattccactt aatgtcggta aaaatcccgt
     1681 attaacaacc acattaaatg atgatcaact tttaaagtta tcagagcagg ttcagcatga
     1741 ttcagaaatc attgctcgcc ttactgacaa aaagatgaaa gatctttcag agatgagtca
     1801 cacccttact ccagagaaca ctctggatat ttccagtctt tcttctaatg ctgtttcttt
     1861 aattattagt gtagccgttc tactttctgc tctccgcact gcagaaacta aattgggctc
     1921 tcaattgtca ttgattgcgt tcgatgctac aaaatcagct gcagagaaca ttgttcggca
     1981 aggcctggca gccctatcat caagcattac tggagcagtc acacaagtag gtataacggg
     2041 tatcggtgcc aaaaaaacgc attcagggat tagcgaccaa aaaggagcct taagaaagaa
     2101 ccttgccact gctcaatctc ttgaaaaaga gcttgcaggt tctaaattag ggttaaataa
     2161 acaaatagat acaaatatca cctcaccaca aactaactct agcacaaaat ttttaggtaa
     2221 aaataaactg gcgccagata atatatccct gtcaactgaa cataaaactt ctcttagttc
     2281 tcccgatatt tctttgcagg ataaaattga cacccagaga agaacttacg agctcaatac
     2341 cctttctgcg cagcaaaaac aaaatattgg ccgtgcaaca atggacacat cagccgttgc
     2401 tggtaatata tccacatcag gagggcgtta tgcatctgct cttgaagaag aagaacaact
     2461 aatcagtcag gccagcagta aacaagcaga ggaagcatcc caagtatcta aagaagcatc
     2521 ccaagcgaca aatcaattaa tacaaaaatt attgaatata attgacagca tcacccaatc
     2581 aaggaattcg acagccagtc agattgctgg taacattcga gcttaatata tccaagagcc
     2641 ataataatat atggctcttc ctgtaaggaa ataaccatga atataacaac tctgactaat
     2701 agtatttcca cctcatcatt cagtccaaac aataccaacg gttcatcaac cgaaacagtt
     2761 aattctgata taaaaacaac gaccagttct catcctgtaa gttcccttac tatgctcaac
     2821 gacacccttc ataatatcag aacaacaaat caggcattaa agaaagagct ttcacaaaaa
     2881 acgttgacta aaacatcgct agaagaaata gcattacatt catctcagat tagcatggat
     2941 gtaaataaat ccgctcaact attggatatt ctttccagga acgaatatcc aattaataaa
     3001 gacgcaagag aattattaca ttcagccccg aaagaagccg agcttgatgg agatcaaatg
     3061 atatctcata gagaactgtg ggctaaaatt gcaaactcca tcaatgatat taatgaacag
     3121 tatctgaaag tatatgaaca tgccgttagt tcatatactc aaatgtatca agattttagc
     3181 gctgttcttt ccagtcttgc cggctggatc tctcccggag gtaacgacgg aaactccgtg
     3241 aaattacaag tcaactcgct taaaaaagca ttggaagaac tcaagaaaaa atatgaagat
     3301 aaaccgctat atccagcaac taatactgtt agtcagaaag aagcagataa atggcttaca
     3361 gaattaggtg gaacaatcgg caaggtatct aaaaaaaacg ggggatatgt tgtcaatata
     3421 aacatgaccc caatagacaa tatgttaaaa agcttaaata atctaggtgg aaatggcgag
     3481 gttgtgctag ataatgcaaa atatcaggca tggaatgccg gattctctgc cgaagatgaa
     3541 acaatgaaaa ataatcttca aactttagtt caaaaataca gtaatgccaa tagtattttt
     3601 gataatttag taaaggtttt gagtagtaca ataagctcat gtacagatac agataaactt
     3661 tttctccatt tctgaggtgc gccatgcata atgtaaataa tactcaagcg ccaacattct
     3721 tatataaggc aacttcacca tcatcaacag aatacagcga gttaaaaagc aaaatatccg
     3781 atatccatag ttcgcaaact tctctaaaaa caccagcatc agtgtctgaa aaagaaagct
     3841 ttgcaacgtc ttttaatcag aaatgtcttg attttttatt ttcttcctca gggaaagaag
     3901 atgtgttaag aagcatttat tccaactcaa tgaatgcgta tgccaaaagc gagattctcg
     3961 aattttcaaa tgttttgtac tccttagtac atcaaaatgg tcttaatttt gaaaacgaaa
     4021 agggacttca aaaaattgtc gcacagtatt cggaactaat tataaaagat aaattatccc
     4081 aagattctgc ctttggacca tggtcggcaa agaataagaa actccatcaa ttacgacaaa
     4141 acattgagca cagacttgca ctattagcac aacaacacac atctggtgaa gctttatcat
     4201 tgggacaaaa actcctcaat actgaagtat catcatttat caagaataat attcttgctg
     4261 aattaaagtt aagtaatgaa actgtttcat ctctcaaact agatgattta gttgacgcac
     4321 aggcaaaact tgcctttgat agtttgcgca atcaacgt
//
LOCUS       DAZSBF010000285         4329 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZSBF010000285 DAZSBF010000000
VERSION     DAZSBF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.290"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            147..3239
                     /gene="ebgA"
                     /locus_tag="ACHXWD_003943"
                     /pseudo
     CDS             147..3239
                     /gene="ebgA"
                     /locus_tag="ACHXWD_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
     gene            3236..3685
                     /locus_tag="ACHXWD_003944"
     CDS             3236..3685
                     /locus_tag="ACHXWD_003944"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR7622704.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKMVLKVTIEDGYFHNK"
     gene            3748..>4329
                     /locus_tag="ACHXWD_003945"
     CDS             3748..>4329
                     /locus_tag="ACHXWD_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR7622705.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
BASE COUNT         1043 a         1167 c         1089 g         1030 t
ORIGIN      
        1 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgtcactttt
       61 tagtaaaaat tttactaaac tccccagcaa ttacacaaac taccatcacc atgaatggtt
      121 ccgatttctc tctaccggga ggccctatga atcgctggga aaacattcag ctcacccacg
      181 aaaaccgact tgcgccacgt gcgtactttt tttcatatga ttctgttgcg caagcgcgta
      241 cctttgcccg cgaaaccagc agcctgtttc tgcccttaag cggtcagtgg aatttccact
      301 tttttgacca cccgctgcaa gtgcctgaag ccttcacctc tgagttaatg gctgactggg
      361 ggcatattac cgtccccgcc atgtggcaaa tggaaggtca cggcaaactg caatataccg
      421 acgaaggttt tccgttcccc atcgatgtgc cgtttgttcc cagcgataac ccaaccggtg
      481 cctatcaacg tattttcacc ctcagcgacg gctggcaggg taaacagacg ctgattaaat
      541 ttgacggcgt cgaaacctat tttgaagtct acgttaacgg tcagtatgtg ggtttcagca
      601 agggcagtcg cctgaccgca gagtttgaca tcagcgcgat ggttaaaacc ggcgacaacc
      661 tgttgtgtgt gcgcgtgatg caatgggcgg actctaccta cgtggaagac caggatatgt
      721 ggtggtcagc ggggatcttc cgcgatgttt atctgatcgg aaaacaacta acacatatta
      781 acgatttcac cgtgcgtacc gactttgacg aagcctattg cgatgccacg ctttcctgcg
      841 aagtggtgct ggaaaatctc gccgcctccc ctgtcgtcac gacgctggaa tataccctgt
      901 ttgatggcga acgcgtggtg cacagcagcg ccattgatca tttggcaatt gaaaaactga
      961 ccagcgccag ctttgctttt actgtcgaac agccgcagca atggtcagca gaatcccctt
     1021 atctttacca tctggtcatg acgctgaaag acgccaacgg caacgttctg gaagtggtac
     1081 cacaacgcgt tggcttccgt gatatcaaag tgcgcgacgg tctgttctgg atcaataacc
     1141 gttatgtgat gctgcacggc gtcaaccgtc acgacaacga tcatcgcaaa ggccgcgccg
     1201 ttggaatgga tcgcgtcgag aaagatctcc agttgatgaa gcagcacaac atcaactccg
     1261 tgcgtaccgc tcactacccg aacgatccgc gtttttacga actgtgtgat atctacggcc
     1321 tgtttgtgat ggcggaaacc gacgtcgaat cgcacggctt tgctaatgtc ggcgatatca
     1381 gccgtattac cgacgatccg cagtgggaaa aagtctacgt cgagcgcatt gttcgccata
     1441 ttcacgcgca gaaaaaccat ccgtcgatca tcatctggtc gctgggcaat gaatccggct
     1501 atggctgtaa catccgcgcg atgtaccacg cagcgaaggc gctggatgac acgcgactgg
     1561 tgcattacga agaagatcgc gatgctgaag tggtcgatat tatttccacc atgtacaccc
     1621 gcgtgccgct gatgaatgag tttggtgaat acccgcatcc gaagccgcgc atcatctgtg
     1681 aatatgctca tgcgatgggg aacggaccgg gcgggctgac ggagtaccag aacgtcttct
     1741 ataagcacga ttgcattcag ggtcattatg tctgggagtg gtgcgaccac gggatccagg
     1801 cacaggacga ccacggcaat gtctggtata aattcggcgg cgactacggc gactatccca
     1861 acaactataa cttctgtctt gatggtttga tctattccga tcagacgccg ggaccgggcc
     1921 tgaaagagta caaacaggtt atcgcgccgg taaaaatcca cgcgcgggat ctgactcgcg
     1981 gcgagttgaa agtcgaaaat aaactgtggt ttaccacgct tgatgactac cccctgcacg
     2041 cagaggtgcg cgccgaaggt gaaacgctcg cgacgcagca gattaaactg cgcgacgttg
     2101 cgccgaacag cgaagccccc ttgcagatca cgctgccgca gctggacgcc cgcgaagcgt
     2161 tcctcaacat tacggtgacc aaagattccc gcacccgcta cagcgaagcc gggcattcta
     2221 tcgccactta tcagttcccg ctgaaggaaa acaccgcgca gccagtgcct ttcgcaccaa
     2281 ataatgcgcg tccgctgacg ctggaagacg atcgtttgag ctgcaccgtt cgcggctaca
     2341 acttcgcgat caccttctca aaaatgagtg gcaaaccgac atcctggcag gtaaatggcg
     2401 agtcgctgct gacccgcgag ccaaagatca acttcttcaa gccgatgatc gacaaccaca
     2461 agcaggagta cgaagggttg tggcagccga atcatttgca gatcatgcag gaacatctgc
     2521 gcgactttgc cgtagaacag agcgagggtg aagtgttgat catcagccgc acggttattg
     2581 ccccgccggt gtttgatttc ggaatgcgct gcacctacat ctggcgcatc gctgccgatg
     2641 gccaggttaa cgtggcgctt tccggcgagc gttacggcga ctatccgcac atcattccgt
     2701 gcatcggttt caccatggga attaacggcg aatacgatca ggtggcgtat tacggtcgtg
     2761 gaccgggcga aaactacgcc gacagccagc aggctaacat catcgatatc tggcgcagca
     2821 ccgtcgatgc catgttcgag aactatccct tcccgcagaa caacggtaac cgtcagcatg
     2881 tccgctggac ggcactgact aaccgccacg gtaacggtct gctggtggtt ccgcagcgcc
     2941 caattaactt cagcgcttgg cactatacct aggaaaacat ccacgctgcc cagcactgta
     3001 acgagctgca gcgcagtgat gacatcaccc tgaacctcga tcaccagctg cttggcctcg
     3061 gctccaactc ctggggcagc gaggtgctgg actcctggcg cgtctggttc cgtgacttca
     3121 gctacggctt tacgttgctg ccggtttctg gcggagaagc taccgcgcaa agcctggcgt
     3181 cgtatgagtt cggcgcaggg ttcttttcca cgaatttgca cagcgagaat aagcaatgag
     3241 gatcatcgat aacttagaac agttccgcca gatttacgcc tctggcaaga agtggcaacg
     3301 ctgcgttgaa gcgattgaaa atatcgacaa cattcagcct ggcgtcgccc actccatcgg
     3361 tgactcattg acttaccgcg tggagacaga ctccgcgacc gatgcgctat ttaccgggca
     3421 tcgacgctat tttgaagtgc attactacct gcaagggcag caaaaaattg aatatgcgcc
     3481 gaaagagaca ttacaggtag tggaatatta tcgtgatgaa actgaccgtg aatatttaaa
     3541 aggctgcgga gaaaccgttg aggtccacga agggcaaatc gttatttgcg atatccatga
     3601 agcgtatcgg tttatctgca ataacgcggt caaaaaaatg gttctcaaag tcaccatcga
     3661 agatggttat ttccataaca aataacaact acggcggcaa aaggagtttg ccgccaccgc
     3721 taccctactc attttcggag atgtgttatg tctgatacca aacgtaatac aatcggcaaa
     3781 ttcggcttgc tctcgctgac ttttgccgcc gtttacagct ttaacaacgt tatcaataat
     3841 aatattgatc ttggactggc ctcggcaccg atgtttttcc tcgcgacgat tttttatttt
     3901 attcccttct gtctgatcat cgcagaattt gtttcgttaa ataaaaactc agaagccggt
     3961 gtctacgcat gggtaaaaag ttcgctgggc ggacgttggg catttattac tgcctatacc
     4021 tactggttcg taaacctgtt ctttttcacc tcactgttgc cgcgcgttat tgcttatgct
     4081 tcgtatgcct tcctcggata cgaatatatt atgacgccgg ttgccaccac cattatcagt
     4141 atggtgctgt tcgccttctc cacctgggtt tccaccaacg gggcgaaaat gttggggcca
     4201 attacctccg tcacttcaac gctgatgctg ctgttaacgc tctcctacat tttactggca
     4261 ggtacggcgc tggttggcgg cgtacagcct gctgacgcca tcaccgttga cgcgatgatc
     4321 ccgaacttc
//
LOCUS       DAZSBF010000286         4309 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZSBF010000286 DAZSBF010000000
VERSION     DAZSBF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.291"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            162..1844
                     /locus_tag="ACHXWD_003946"
     CDS             162..1844
                     /locus_tag="ACHXWD_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR7622706.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1896..3053
                     /locus_tag="ACHXWD_003947"
     CDS             1896..3053
                     /locus_tag="ACHXWD_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR7622707.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3019..3141)
                     /locus_tag="ACHXWD_003948"
     CDS             complement(3019..3141)
                     /locus_tag="ACHXWD_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622708.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3356..>4309
                     /locus_tag="ACHXWD_003949"
     CDS             3356..>4309
                     /locus_tag="ACHXWD_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
                     /protein_id="HFR7622709.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAF"
BASE COUNT         1297 a          916 c          851 g         1245 t
ORIGIN      
        1 tacttaatag aacagaaagt aatgtcaacg ctttatggac tgttatttcc ctttttttag
       61 ctaaatctgc tatctcttta tgtgactaac ttcacttaca tccacttatt tctcttcgta
      121 aaattacttc ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga
      181 aaagtgtcgt aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg
      241 ctcatgcggc agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagatt
      301 ttacgccgac tgaatacagt accaaaggaa agccaaatat tatcgtacta accatggatg
      361 atcttggtta tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa
      421 atcgtgaagt tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcacaaa
      481 aatcaacgcc gacgctcctt tcattaatgg atgaaggcgt acgttttact aacggctatg
      541 tggcacacgg tgtttccggc ccctcccgtg ccgcaataat gaccggtcga gctcccgccc
      601 gctttggtgt ctattccaat accgatgctc aggatggtat tccgctaaca gaaactttct
      661 tgcctgaatt attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt
      721 caaaaatcag taatgtgccg gtaccggaag ataaacaaac gcgtgactat catgacaact
      781 tcaccacatt ttctgcggaa gaatggcaac ctcaaaaccg tggctttgat tactttatgg
      841 gattccacgc tgcaggaacg gcatattaca actccccttc actgttcaaa aatcgtgaac
      901 gtgtccccgc aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg
      961 atcgtgccaa aacacttgac cagcctttta tgctttacct ggcttataat gctccgcacc
     1021 taccaaatga taatcctgca ccggatcaat atcagaagca atttaatacc ggtagtcaaa
     1081 cagcagataa ctactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg
     1141 aacaactgaa gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg
     1201 gtgcggttat cgatggtcct ctgccgctga acggggcgca aaaaggctat aagagtcaga
     1261 cctatcctgg cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg
     1321 gtaattatga caagctgatt tccgcaatgg atttctaccc aacagctctt gatgcagccg
     1381 atatcagcat tccaaaagac cttaagctgg atggtgtttc cttgctgccc tggttgcaag
     1441 ataagaaaca aggcgcgcca cataaaaatc tgacctggat aacctcttat tctcactggt
     1501 ttgatgagga aaatattcca ttctgggata attaccacaa atttgttcgc catcagtcag
     1561 acgattaccc gcataacccc aacactgagg acttaagcca attctcttat acggtgagaa
     1621 ataacgatta ttcgcttgtc tatacagtag aaaacaatca gttaggtctc tacaaactga
     1681 cggatctaca gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc
     1741 aaggcgtggt aagagagttt atcgacagca gccagccacc gcttagcgag gtaaatcagg
     1801 agaagtttaa caatatcaag aaagcactaa gcgaagcgaa ataactaaac cttcatgcgg
     1861 cggatttttc cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca
     1921 gttttcaatg taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta
     1981 ctcatgaaaa atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgaag
     2041 cgtctggcaa tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg
     2101 attttttccg taaagttatt cactatcaac aacgctatgc aggccaaaaa cgtattttta
     2161 atgcattaca aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac
     2221 atgaatttct ggttggtatc tcgatcgatg gccccctgga gttacatgac cgttacagac
     2281 gcagtaattc aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat
     2341 catatcaagt agagtttaat acgttaaccg tcattaataa cgttaatgtc cattaccctc
     2401 ttgaggttta tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc
     2461 tagaaaccgg gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca
     2521 ttgatttttc tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcaat
     2581 gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt tgaaagcttt gtcagccgtt
     2641 ttttggggaa tgggcataca agttgtattt tccaggagtc ctgcaaggat aatctggttg
     2701 ttgaaagtaa tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg
     2761 gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa
     2821 aacggatttc agcgaaatgt cagcaatgtg catataaacc tatctgcaat ggcggttgtc
     2881 ctaagcatcg tattactaaa gtaaacaatg agactgtttc ttatttttgc gaaggttata
     2941 aaatcctttt ttcaaccatg gtaccttata tgaacgccat ggttgagtta gctaagaaca
     3001 gagtaccgct ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct
     3061 ctcttttaac tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat
     3121 tcctgtcggg tgtttcgtca tcatcatctt cccttgcgaa aatcctggct tacgtggtat
     3181 gcgtcagggc attagcaata cccttgatta tcaatagcat aaccataacc ataaccataa
     3241 ccactgaaaa ttaaaaattt ctatatattt catattgtta aatgatactt aaataaattt
     3301 catcagtaaa tcattgccat agataatcat tggatggcac aatcaatttt cgctaatgat
     3361 aaccattccc attacgtttt atatgttgat agcaaagtat ctctgtctgc tgaagccctt
     3421 ttggctgcgt aagaataata aaacttcagt gttgttaatc attattatcc tcgcaatgat
     3481 ccttggcgtg gttaaaattc aggtctggtt aaacgactgg aataatgatt ttttcaatgc
     3541 gctaagccag aaagaaaccg ataagctttg gcagcttgtt ctttggttcc ctgccctgct
     3601 ggggatcttt gtattaatat ccgtaaacaa aacatggtta attaaactac tgactattcg
     3661 ctggcgcgaa tggttaacag attactacct taatcgctgg tttgcagata aaaactacta
     3721 cttcacgcag atctacggcg agcataaaaa tacagataac cctgaccaac gtatcgctga
     3781 agacattctc ttactgatca gcaaaacgct cagtctttca tttggcttca tccagtcact
     3841 tagcatgctg atcaccttta ccgttattct ttggcaaagc gcgggtacgc tctcattcac
     3901 tgttggtgga acagaatgga atatccaggg gtatatggtc tataccgtcg tgctcattgt
     3961 gatcggtgga actttattta cccataaggt gggtaaacgt attcgtccgc ttaatgtgga
     4021 aaaacaacgt agcgaagcga catttcgaac taatcttgtg cagcataata agcagacaga
     4081 actgattgcg ctaagcaatg ctgagtctct acaacgccag gagttgcgcg acaattttca
     4141 taccattaaa gagaactggc atcgtttaat gaatcgtcag cggtggcttg attactggca
     4201 aaatatttat tcgcgctcgc ttagcgttct tccctacttt ctgttattgc cgcagtttat
     4261 tagcgggcag atcaatctgg gcggactgat gaaatcgcgc caggcattt
//
LOCUS       DAZSBF010000287         4288 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZSBF010000287 DAZSBF010000000
VERSION     DAZSBF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.292"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            224..1065
                     /locus_tag="ACHXWD_003950"
                     /pseudo
     CDS             224..1065
                     /locus_tag="ACHXWD_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            1068..2156
                     /gene="capU"
                     /locus_tag="ACHXWD_003951"
     CDS             1068..2156
                     /gene="capU"
                     /locus_tag="ACHXWD_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFR7622710.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2161..3111
                     /gene="virK"
                     /locus_tag="ACHXWD_003952"
     CDS             2161..3111
                     /gene="virK"
                     /locus_tag="ACHXWD_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFR7622711.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            3176..4120
                     /gene="lpxM"
                     /locus_tag="ACHXWD_003953"
                     /gene_synonym="msbB"
     CDS             3176..4120
                     /gene="lpxM"
                     /locus_tag="ACHXWD_003953"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR7622712.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         1224 a          799 c          980 g         1285 t
ORIGIN      
        1 tcaggtagcc aaattcagtg tgccacctca aggttcttag tgagtcgttt ttaaaagata
       61 tgataatata tcattgttta tgaattgttg atgtttaatt tagtaaatta tatcaaatat
      121 gccatgggtt tgctatgtta ttcatccagt atctggaaat aaaatgcacc agctttattg
      181 ggttttcctt ttgtctctgc ttactaggtg ccagaaactt taaatgttaa acgaaggggg
      241 cattttgttc aaagccaatc atgttccggt attaatgtat catcatgtca gtcattgccc
      301 cggactggtt acactttctc ccgtgacatt ccggaagcaa atgaaatggt tggctgaaaa
      361 caactggaaa acactgtcat cagatgaact ggagtttttt tatcggggcg gaaaactccc
      421 ccggaaaagc gttatgttga catttgatga tggatacctt gacaactggt ttcaggtata
      481 tcctttatta aaagagttta atctcaaagc acatattttt ttaattaccg gttttattgg
      541 gaatggtccg gttcgtcact ctcctggaaa ggaatattct catcgcgatt gcgaacatca
      601 aatagcgaca gggaatgctg ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt
      661 gcaatctggt ctggtcgaat ttcatgttca tacgcatact catacacgct gggataaaaa
      721 gttcagttca cgagaagaac agtgtaaaca tcttcggcag gacttgttga gcgggagaga
      781 atatttgaaa gaaatgacag gaaatgcagt aaacatttgt gttggccgga aggttattat
      841 aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata tacaacagaa
      901 agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag caccaaagaa
      961 cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacacc gtttttttca
     1021 tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg aatatactat
     1081 ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct caaatgaagg
     1141 ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc aaaattgctt
     1201 ttgaagccag taaattggga attgatatca cattcgcgtt atttcgaaac agtcttcaca
     1261 tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat gcaatcgttt
     1321 gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact tggaaacatc
     1381 cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt ttttcaataa
     1441 atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat ctggagcagg
     1501 aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag aaattatacg
     1561 ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc cgaaggggat
     1621 gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa tttatgttga
     1681 atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt gtggggtctg
     1741 gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc atgcatgatg
     1801 atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt gccagtctgg
     1861 tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca tcggcatttt
     1921 ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag aacaaccaga
     1981 ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg aatgattttt
     2041 ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat atagaagagc
     2101 ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac aagtaacaat
     2161 atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt tttttcctca
     2221 gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat tttgcgatgt
     2281 gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt gcagtgcatt
     2341 gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc ttatttgcat
     2401 aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg ctttgtgcaa
     2461 aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc attagtccaa
     2521 ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg ttttgaccgg
     2581 gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg gttaacattt
     2641 tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca gggagccccc
     2701 aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg gcttttccca
     2761 aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt ctctgagtgt
     2821 ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta tcagaaacgc
     2881 aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa aacctgtggt
     2941 gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat agctagtaaa
     3001 aaacgctctg agtatcgaaa acgatacgct ttgttggatt atatccatga aactgctatc
     3061 cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta aggtttgatg
     3121 tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact aaataatgaa
     3181 aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc ctgtttactg
     3241 gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc cttcattcag
     3301 agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa aagctcgtcg
     3361 cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag aacgggaaat
     3421 aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg cagaactggc
     3481 aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg aaattcttga
     3541 ggaaatccgg cacaataaca gaaatgtgat ttttctggtt ccccatggct ggagcgtgga
     3601 tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt ttcatcagca
     3661 acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg gggggcgctt
     3721 acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg gatactgggg
     3781 gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg atttttttgc
     3841 gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc aggctatgat
     3901 tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg aagttcggcc
     3961 accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac aaatgaacaa
     4021 aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt taaaattgtt
     4081 aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag tacaggtatt
     4141 tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg tgtgttgccc
     4201 gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatgc ctaaatagct
     4261 gcgcttaata ccgctacact tttgccag
//
LOCUS       DAZSBF010000288         4251 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZSBF010000288 DAZSBF010000000
VERSION     DAZSBF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.293"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..331
                     /locus_tag="ACHXWD_003954"
     CDS             <1..331
                     /locus_tag="ACHXWD_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR7622713.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHXWD_003955"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="ACHXWD_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR7622714.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHXWD_003956"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="ACHXWD_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR7622715.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHXWD_003957"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="ACHXWD_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR7622716.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHXWD_003958"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="ACHXWD_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR7622717.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4035..>4251)
                     /locus_tag="ACHXWD_003959"
     CDS             complement(4035..>4251)
                     /locus_tag="ACHXWD_003959"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622718.1"
                     /translation="NPLFFCPFFAPSTAFCPIFAINNFPHKVISKPNSCSTFFLNIMS
                     PAGNNLIPVLGLKAVNDNHFHFVWVML"
BASE COUNT         1152 a         1041 c         1063 g          995 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag gggcaaaaaa agagtgggtt a
//
LOCUS       DAZSBF010000289         4192 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZSBF010000289 DAZSBF010000000
VERSION     DAZSBF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.294"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHXWD_003960"
     CDS             complement(<1..215)
                     /locus_tag="ACHXWD_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR7622719.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHXWD_003961"
     tRNA            complement(330..405)
                     /locus_tag="ACHXWD_003961"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHXWD_003962"
     tRNA            complement(412..486)
                     /locus_tag="ACHXWD_003962"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHXWD_003963"
     tRNA            complement(603..687)
                     /locus_tag="ACHXWD_003963"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHXWD_003964"
     tRNA            complement(696..771)
                     /locus_tag="ACHXWD_003964"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHXWD_003965"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHXWD_003965"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR7622720.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHXWD_003966"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHXWD_003966"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR7622721.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHXWD_003967"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHXWD_003967"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR7622722.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1040 c          936 g         1136 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatct tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 acttcccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAZSBF010000290         4189 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZSBF010000290 DAZSBF010000000
VERSION     DAZSBF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.295"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            172..441
                     /locus_tag="ACHXWD_003968"
     CDS             172..441
                     /locus_tag="ACHXWD_003968"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7622723.1"
                     /translation="MWCFFNLFGVLIPIDERNLTRERTQVGLQAARARGRKGGRPKTL
                     SKDKQALAVQLYNEKKHTVAQICVLMGISRPTLYKYIESARLFKK"
     gene            complement(483..889)
                     /locus_tag="ACHXWD_003969"
                     /pseudo
     CDS             complement(483..889)
                     /locus_tag="ACHXWD_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    complement(492..608)
                     /locus_tag="ACHXWD_003969"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(1053..1554)
                     /gene="aqpZ"
                     /locus_tag="ACHXWD_003970"
                     /pseudo
     CDS             complement(1053..1554)
                     /gene="aqpZ"
                     /locus_tag="ACHXWD_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014728363.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
     gene            complement(1673..3592)
                     /locus_tag="ACHXWD_003971"
     CDS             complement(1673..3592)
                     /locus_tag="ACHXWD_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005143902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFR7622724.1"
                     /translation="MIDRILELPRIVKRGIIICIDVVMVIFSFWLSYWLRLDEQTAFL
                     SAPMWFAAAILTIFTVFIFIRIGLYRAVLRYVSAKIMLLIPVGILASTLSLVVISYSL
                     SIMLPRTVVGIYFLVLLLLTSGSRLLFRMILNYGVKGSAPVLIYGAGESGRQLLPALM
                     QAKEYFPVAFVDDNPRLHKAVIHGVTVYPSDKLSYLVDRYGIKKILLAMPSVSKSQRQ
                     KVITRLEHLPCEVLSIPGMVDLVEGRAQISNLKKVSIDDLLGRDPVAPDAKLMAENIT
                     GKAVMVTGAGGSIGSELCRQIVRYKPAKLVLFELSEYALYAIEKELSALCDKEVLNVP
                     VIPLLGSVQRQNRLQMVMKSFGIQTVYHAAAYKHVPLVEHNVVEGVRNNVFGTLYCAE
                     SAIESGVETFVLISTDKAVRPTNTMGTTKRLAELVLQALSARQSQTRFCMVRFGNVLG
                     SSGSVVPLFEKQIAQGGPVTLTHRDIIRYFMTIPEASQLVIQAGAMGHGGDVFVLDMG
                     DPVKIYDLAKRMIRLSGLSVRDDKNPDGDIAIEVTGLRPGEKLYEELLIGDSVQGTSH
                     PRIMTANEVMLPWQDLSLLLKELDQACHDFDHERIRSLLLQAPAAFNPTDDICDLVWQ
                     QKKSLLSQASNVIRL"
     gene            complement(3607..4188)
                     /locus_tag="ACHXWD_003972"
     CDS             complement(3607..4188)
                     /locus_tag="ACHXWD_003972"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFR7622725.1"
                     /translation="MFDVIVAGLGLLFLFPVFIIVSMLIVADSKGGVFFRQYRVGRFG
                     KDFRIHKFRTMFIDSEKKGRITVGQDARVTRVGWYLRKYKIDELPQLIDVLSGTMSLV
                     GPRPEVREFIDEYPDDIREKVLSVRPGITDLASIEMVDENEILSSYDDPRRAYIDIIL
                     PIKQRYYLDYVANNSVKYDCVIIWKTIIKILSR"
BASE COUNT         1246 a         1051 c          867 g         1025 t
ORIGIN      
        1 gctggcccgg atgcggtggt gcggctgaga gccgcagagg ggaagttgcg tccattttga
       61 gggaaacgga gcaaaaaacc gccattcata ccctgaatca atcagagcca tccagtttaa
      121 tcgtcggttc cgttcatatg gagcaaaagt ggtgttgaag aaacatccgt tttgtggtgt
      181 ttttttaatc tttttggggt tttaattcct atcgatgaac gcaacctgac aagagagaga
      241 actcaggtcg ggcttcaagc agcacgagcc agaggacgta agggcggcag gccgaaaaca
      301 ctgagtaagg ataaacaggc ccttgctgtt caactctata acgagaaaaa acatacagtt
      361 gcgcagattt gtgtgttgat ggggatatcg aggccaacgc tgtacaaata catagagtca
      421 gccaggttgt tcaagaaatg aacttagcgt acgttttcgt acggttgggc ttcaaatccc
      481 taatacgttg acggggcaat atgcagttcg ctgcatagcg gtccgacccc gtactgctca
      541 cgcagcttat ccagcagtgg catcattttt tccagaggcg gtcgaactcc gccttcgcaa
      601 aataagcgga agcttggcga aggatatcgt tactgcggcg cagttcacga ttttcacgct
      661 ccagctcttt cagacgctga cgttcagcgg tggtgagccc tccatcaccg cccccggtat
      721 cccgctcatg ctggcgaacc cggacacgca gagtctccgg cgtacagcca atctttggag
      781 caatggaaca aattgtcgcc cattgtgagt catattcgcc ctgactttcc agaaccatac
      841 ggactgcccg ttgacggact tcgggggaaa aacgagtatt tttagtcatc ctgtttacct
      901 ctttctcagg aagtttagtc tccaggattc ccggggcggt tcatttgagg atcgccatga
      961 tttggctgtc ggtaaatttt gatgttttca taaagaatct cctgcgttta tggtacgaga
     1021 aaattctact tttgacgctg gttaatttac ggggtgatta ccagaccaat cgccagtggc
     1081 gcaaagcccg caggagcgcg tttttctgtc gctccggaca aaaaacgcac tgaggacaaa
     1141 ctcgctcagc atacaggcgc ttaacgcata gccgccaggt gaatggaggc cataaccatt
     1201 agccgcaaaa ccgctggttg tcgcatcaaa cccagcctta ccgctggcga taccatacag
     1261 cactgccgca gcggcaatac cgccgataac ctgagcgata atgtaaggta acacgcgcgc
     1321 tgctgggaag cgtccaccgg cccacagacc caaggtcacc gcggggttaa aatgcgcacc
     1381 agagatgtgc ccgaccgcat aagccatggt gactactgtc agaccaaacg ccaacgccac
     1441 gcccaaaaag cctataccta actgtgggaa agcagcagag atcaaggcgc taccacagcc
     1501 acccagaacc agccagaaag tgccgaaaaa ttcggcgcta aaaggtttaa acatacactg
     1561 atgtcctgtt tgttaaagag caggcatcag ggtaaaggcg gtcacgatgt accgcctagc
     1621 ggggatttgt aagagaggtg aagaattggt gtggaaggtt aaacctaagc aatcacaggc
     1681 gaatgacatt gctcgcttgt gataacagcg attttttctg ctgccaaact agatcgcaaa
     1741 tatcatcagt tggattgaat gccgctggtg cttgtaacaa caaactgcga attcgctcat
     1801 gatcaaagtc atgacaagct tgatccagct ctttaagtaa gagcgataga tcctgccacg
     1861 gtagcatcac ttcgttggcc gtcataattc gtggatgaga ggtaccttga actgaatcac
     1921 caatcagtaa ttcttcatac agtttctcac ctggacgtaa tcccgtaact tcaatggcaa
     1981 tatcgccatc tggattttta tcatcccgta cactcaagcc acttaaccgg atcatgcgtt
     2041 tggctaagtc ataaatcttg accggatcgc ccatgtctaa gacaaagaca tcgccgccat
     2101 gccccatcgc ccccgcttga atcaccaact gtgatgcttc cggaattgtc atgaaatagc
     2161 gaataatgtc acgatgagtc aaggtaactg gcccaccttg ggcaatctgt ttttcaaaca
     2221 acggcacgac agagcccgaa gaaccgagta cattaccaaa tcgcaccata caaaagcgag
     2281 tttggctttg ccgtgcagac aaagcctgca ataccaattc ggccagacgc ttagttgtcc
     2341 ccatagtgtt ggtcgggcgc accgctttat cggtggaaat caacacaaaa gtttcaacgc
     2401 cactttcgat cgctgactca gcgcagtaca aggtaccaaa cacgttatta cgtacccctt
     2461 ccaccacatt atgctcaacc agaggcacat gtttataagc ggccgcatga taaaccgttt
     2521 gaataccaaa ggacttcatc accatctgta agcgattctg acgctgcacc gagcccaaca
     2581 gagggatcac tggaacattc aaaacttctt tgtcacacag cgccgagagc tctttctcaa
     2641 tagcgtagag ggcatattca gacagttcaa atagaaccaa tttggccggc ttatatcgaa
     2701 caatttgacg acaaagctca gagccgatcg agcctcccgc cccagtgacc ataacggctt
     2761 tgccagtaat gttttcggcc atcaatttgg catcaggagc aaccggatca cgacctagta
     2821 agtcatcaat cgatactttt tttagattac tgatttgtgc tcgaccttcg actaaatcga
     2881 ccatacccgg aatagagaga acttcacacg gtagatgctc taaacgagta atcactttct
     2941 gcctttgtga cttactgacg ctcggcatcg ccaaaagaat tttctttata ccatagcgat
     3001 ctacaaggta actcagttta tccgagggat aaactgttac accatgaatg acagccttat
     3061 gcaagcgagg attatcatcc acaaatgcca caggaaaata ttcttttgcc tgcattaatg
     3121 ctggcaataa ttgtcggcca gattcaccag cgccataaat caaaacaggc gcactaccct
     3181 taactccata gttaagtatc attctaaaaa gcaatctaga gcctgatgtc agtaaaagta
     3241 aaaccaaaaa ataaattccg acaacagtgc gcggcaacat tatggatagc gaatatgata
     3301 taacgacaag agataacgtt gaggccagaa taccaactgg tattagcaac attatctttg
     3361 cactaacata ccgtaagact gcccgataaa gcccaatcct gataaatata aacacggtaa
     3421 atatggtaag aatagctgca gcaaaccaca tcggtgcact aagaaaagcc gtttgctcat
     3481 caagcctcaa ccaataagac aaccaaaatg agaatatcac cataactaca tcaatgcaga
     3541 tgatgatacc tctcttaaca attcttggca gctccaatat tctatcaatc atcctacact
     3601 accttattat cgcgacaaaa tcttaataat agttttccaa attatcacac aatcatactt
     3661 tactgaattg ttagcaacat aatctaaata atatctttgc ttgattggaa gaattatatc
     3721 tatataagcc ctacgtgggt catcataact agacaaaatc tcattttcat ctaccatttc
     3781 tatagatgct aagtcagtta tccctggcct aaccgataaa actttttccc ttatatcatc
     3841 aggatactca tcaataaact ccctcacttc cggtcttggg ccaaccaaac tcattgttcc
     3901 agaaagaaca tctatcaatt gaggaagctc atcgattttg tacttccgta aataccatcc
     3961 aactctggtt acccgagcat cttgaccaac tgttatccgt cctttttttt ctgaatcgat
     4021 aaacatcgtt ctaaatttat gtatcctaaa atctttccca aatctcccaa ctctatactg
     4081 cctaaaaaaa accccccctt tagaatcagc aacaattaac attgacacaa tgataaaaac
     4141 aggaaataga aaaagcaggc ctaagcctgc cacgataaca tcaaaaata
//
LOCUS       DAZSBF010000291         4186 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZSBF010000291 DAZSBF010000000
VERSION     DAZSBF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.296"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..324
                     /locus_tag="ACHXWD_003973"
     CDS             <1..324
                     /locus_tag="ACHXWD_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7622726.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="ACHXWD_003974"
     CDS             551..1816
                     /locus_tag="ACHXWD_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7622727.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="ACHXWD_003975"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="ACHXWD_003975"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR7622728.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..3483
                     /gene="aroD"
                     /locus_tag="ACHXWD_003976"
     CDS             2725..3483
                     /gene="aroD"
                     /locus_tag="ACHXWD_003976"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR7622729.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3627..>4186
                     /locus_tag="ACHXWD_003977"
     CDS             3627..>4186
                     /locus_tag="ACHXWD_003977"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR7622730.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
BASE COUNT         1092 a          938 c         1005 g         1151 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaatgattta cgcacggtat taactatttt acaccaggca
     3481 taagcaataa tatttcggcg ggaacaccct ccccgccgaa cttaaaaaat atatttaacc
     3541 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tctccatctg gcgtagggaa
     3601 aatcacatct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     3661 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     3721 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattaca gctcttgctg
     3781 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     3841 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     3901 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     3961 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4021 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4081 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     4141 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcg
//
LOCUS       DAZSBF010000292         4144 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZSBF010000292 DAZSBF010000000
VERSION     DAZSBF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.297"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(40..856)
                     /locus_tag="ACHXWD_003978"
                     /pseudo
     CDS             complement(40..856)
                     /locus_tag="ACHXWD_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1174..2451
                     /locus_tag="ACHXWD_003979"
     CDS             1174..2451
                     /locus_tag="ACHXWD_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR7622731.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            2478..3963
                     /locus_tag="ACHXWD_003980"
                     /pseudo
     CDS             2478..3963
                     /locus_tag="ACHXWD_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         1079 a          942 c         1003 g         1120 t
ORIGIN      
        1 taatcattaa taagagaaaa cagaaaccac cgttatggat tagcgcacta aataaccacc
       61 attgaccact aataaatgcc cattgacata atttgaagcc ggacttgcga ggaatacagc
      121 tgcgcccatt aaatcctgag tatcgcccca acggtttgcc ggaatatgat caagaacgcg
      181 ctgattggtt tctggattac tgcgtgtcgc cagcgtaata tctgttgcat aatagccagg
      241 agcaatacca tttacctgaa tattatattg acctagttca tcacaataag ctttggtgaa
      301 tccggcaaga gcatgtttag tggcagaata tgcaggtgac cattgtccac ctaagtaaga
      361 gaacaatgaa cagatattaa taattttgcc gcttttctgc gggatcataa tttttgcagc
      421 ttcatagctt aactcgaatg cggcggtcag gttcacatca atcatcggat cccagtcggc
      481 acgaccgaag tccagcacct tattcagctt acaaataccg gcattgttta ccagaatatc
      541 aactgtaccg aaacgctcac agcaagcagc gataatcttc tgcggcgcgc cttctgcggt
      601 gatatccacc tgcatgaagt ccacctcaac accctgtttt tcaatcattt cctttgtttc
      661 gccgttatct ttcaatcatt tcctttgttt cgccgttatc tttgacgaaa ctaggaataa
      721 agatatttgc gccagctttg gccaacgcca tggcaaatgc ctggcctaaa ccgctattcc
      781 caccggtaac aattgcggtt ttacctttca gtgagaaaaa atccattgag aacgcattga
      841 gagattcgat tgacatagtt agctccaatt tccgtaaggc aaaaaaaaga aaggtaactt
      901 cccccgcgag agggaagtta cctttctcat tatctctggt aactggagat aatttttagc
      961 ataacggcat aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag
     1021 ttttaaaaag cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct
     1081 tattgcaatt actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct
     1141 cttttttttg tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg
     1201 ataaccctcg cgataattag ttttagcggc ggcgttagtt ttgacctggc ttatttacgt
     1261 tatatttttc aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta
     1321 ataatgagta cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc
     1381 gataaatttt cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt
     1441 ctgttaatgg caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg
     1501 ataacgacga ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gctgggcgat
     1561 catagtgagc aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg
     1621 tcgctggcgg tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc
     1681 ctgaaaacag tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg
     1741 ttttttgtta gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc
     1801 cagcttagcg acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg
     1861 atttttggtg tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa
     1921 atgtacggca tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc
     1981 cgcgcgctgt gcggcccact tggaggcatt atcaccacct acagtaaagt gaaatcccct
     2041 acccgcgtga ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc
     2101 acgaactcta acccccaatc ggtcgcgatg gggattggcc tgattttact gctgggattc
     2161 acctgttacg cctcacgcgg gctgtactgg gcctgccccg gcgaagcgag aacaccgtct
     2221 tacattatgg gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc
     2281 gtttacccga ttatcggcca ctggcaagac accctgcccg ctgcagaagc ctaccgcaat
     2341 atgtggctga tgggcatggc ggcgctcggc atggtgattg tctttacctt tttgctgttc
     2401 caaaaaattc gtactgctga tagcgccccc gcaatagcta gcagcaagta agccaggaaa
     2461 attacaggag aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga
     2521 gcacaaaagt ggtgatgtac gatctggaag gtaatgtggt ttgcgaaggt aaaggcttat
     2581 tacagccgat gcacacgcct gatgccgata ctgcagaaca tcctgacgac gatttatggg
     2641 catcattatg ttttgcaggt cacgatttga tgagccagtt tgccgggaat aaagaagata
     2701 ttgttggcat cggcctgggc tccatccgtt gctgccgtgc gttattgaaa gccgacggca
     2761 cgccagctgc gccgttgatt agctggcagg atgcacgcgt tacacgccct tacgaacata
     2821 cgaatcctga cgtggcgtat gtaacctctt tttcgggtta tctgacgcat cgcttaaccg
     2881 gcgaatttaa agataatatc gccaactatt ttggtcagtg gccggtggat tataagacct
     2941 gggcatggag cgaagatgcc gcagtcatgg agaagtttaa tatcccccgt catatgctgt
     3001 ttgatgtgca aatgccaggc actgttcttg ggcatatcac acctcaagcc gcgctggcga
     3061 cgcatttccc ggcaggactg ccggttgttt gtaccaccag tgataaaccg gtagaagctc
     3121 tgggagccgg attactggat gatgaaactg cggtaatttc tcttggcact tacatcgcat
     3181 tgatgatgaa cggcaaagcc ttgccgaaag atccggtggc gtactggccg attatgtctt
     3241 ctattccgca aacattgctg tatgaaggtt acggtattcg caaaggcatg tggacggtga
     3301 gctggctgcg cgacatgtta ggcgagtcgt taattcagga tgccagggcg caggatcttt
     3361 caccggaaga tttactcaac aagaaagcct ctagtgtgcc acctggctgt aatgggctga
     3421 tgacggtgct ggactggctg accaatccgt gggaaccgta caaacgcggg attatgatcg
     3481 gctttgattc cagcatggat tacgcatgga tatatcgttc gatattggaa agcgtggcgc
     3541 tgacgctgaa gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcgaaacatg
     3601 taatcattac tggcggcggt tcgaacagga tctgtttatg cagatttttg ccgacgtgtt
     3661 caaccttccg gcacgccgta acgccattaa cggttgtgca agcctggggg cagcgattaa
     3721 tacaggggta ggtctggggc tatacccgga ttacgcaacg gctgtcgata aaatggttcg
     3781 cgtgaaagat atctttatac cgattgagag caatgccaaa cgctacgacg cgatgaataa
     3841 aggcattttc aaagacctaa ccaaacatac tgatgtgatc ctgaaaaaat cgtatgaagt
     3901 gatgcatggg gaattgggga attggggaat gcggattcga tccagagctg gtcgaatgcg
     3961 tgaataaggt aggccgggcg gaaaactgcc cggcctgaac atacctgaat ggttatcccc
     4021 gctggcgcgg ggaacataag ttcgacaact taaaaaatga taacaagtcc ggtttatccc
     4081 cgctgatgca gggaacataa taaaaacttt aactagttcg gtttatccct gtttttacaa
     4141 ggaa
//
LOCUS       DAZSBF010000293         4132 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZSBF010000293 DAZSBF010000000
VERSION     DAZSBF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.298"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            117..530
                     /locus_tag="ACHXWD_003981"
     CDS             117..530
                     /locus_tag="ACHXWD_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR7622732.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(575..892)
                     /gene="hspQ"
                     /locus_tag="ACHXWD_003982"
     CDS             complement(575..892)
                     /gene="hspQ"
                     /locus_tag="ACHXWD_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR7622733.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(950..2140)
                     /gene="rlmI"
                     /locus_tag="ACHXWD_003983"
                     /pseudo
     CDS             complement(950..2140)
                     /gene="rlmI"
                     /locus_tag="ACHXWD_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2235..2513
                     /gene="yccX"
                     /locus_tag="ACHXWD_003984"
     CDS             2235..2513
                     /gene="yccX"
                     /locus_tag="ACHXWD_003984"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR7622734.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2510..2839)
                     /gene="tusE"
                     /locus_tag="ACHXWD_003985"
     CDS             complement(2510..2839)
                     /gene="tusE"
                     /locus_tag="ACHXWD_003985"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR7622735.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2930..3589)
                     /gene="yccA"
                     /locus_tag="ACHXWD_003986"
     CDS             complement(2930..3589)
                     /gene="yccA"
                     /locus_tag="ACHXWD_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR7622736.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3796..3883)
                     /locus_tag="ACHXWD_003987"
     tRNA            complement(3796..3883)
                     /locus_tag="ACHXWD_003987"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3847..3849),aa:Ser,seq:tga)
BASE COUNT         1100 a         1048 c         1017 g          967 t
ORIGIN      
        1 tcatacttac taacatatag acatatttcc cgcttttgct ctcattcatt cgtattagct
       61 gcatggttgg catgtcggct ttgtcgtaca ctttgttaaa cagtctggag gaaataatga
      121 aagaaaccga tattgcaggc attttaacgt ccactcacac cattgcgctg gtgggggcaa
      181 gcgacaaacc cgatcgtccc agctatcgcg tgatgaaata tctactcgac caggggtatc
      241 acgtcattcc ggtttcgcca aaagttgccg gcaaaacgct gctgggccag caggggtatg
      301 gcacgctggc agacgttccg gaaaaggtcg atatggtcga tgttttccgt aattcagaag
      361 cggcgtgggg cgtggcacag gaagctattg ccattggcgc gaaaaccctc tggatgcaac
      421 tgggggtgat taatgagcaa gcggcagtac tggcacggga tgccgggttg aatgtggtga
      481 tggatcgctg cccggctatt gagatccctc gcctgggcct ggccaaataa aaaatccccg
      541 gaaggcaaaa accttccggg gattcgttca gggatcagtt acgcagacgc ggggcctgga
      601 gttgtttgcg gatggtctgc gccagctcat ccatggatgg ctgttcagga tgttcatcct
      661 gcaattcgct acttagctgg gcttcagcca ggtaggtatg aaccggtagg ccgttatcgt
      721 cctccatcac tacgtgatac cacggagcgg cgcgaagctc gtcatttacc gccagctcat
      781 caggcgacgg ttcagagagc gaataaaccg ggtcgatatc cacgaccact ccgagataac
      841 ctaacaggga atggcggacc tgctggccga taccgaattt gctggcaatc atagtcacct
      901 cccgggaaat ctttaccttt gatataaggg taaaattcca catttcaagt tacatgacgc
      961 gacaggcaaa ccctttcaga tatagccctt ccggataggt agcgatcacc ggatgatcgg
     1021 ctgcctgacg gaactgctct ataaattgta catcacggcc ggcatcaatt gcggcatccg
     1081 cgatgatttt ctgaaataaa tcgctggtca tcagaccgga acaggagaaa gtcaggagaa
     1141 taccgccttc attcagcaac tgaatcgcca gcatgttgat atctttatag ccacgacacg
     1201 cgcccatcaa ctggctttta ttctcaacaa acttcggcgg gtccatcacg ataacgtcaa
     1261 atttttcacc gcgatcgcga taagtacgca gcaatttaaa gacatcatca cggacaaact
     1321 cagccttgct cagatccagt ttgttcagct caacgttctg ccgtgcaata tccagcgctt
     1381 cctaggaggt atcaacgctg acaacctggc tgcaaccgcc catcagtgcc gatacggcga
     1441 aaccaccggt ataggagaaa cagttcagca cacgtttatt ttcaacgtag cggcgggtag
     1501 ccaggcggct gtctcgctgg tccaggtagt agcccgtttt gtgtccgtgc tgaatatcca
     1561 ccagcagctt catgccgtgt tcttcaatcg gcagcagggc aggcggcaac tcgccggtga
     1621 cgaggccctg ggtcagctcc atcccttctt ttttacgtac cgcaacatcg ctgcgatcgt
     1681 aaatcgcaca ttccgggtac agcgtttgca gggcactaat taatgccgcg cgctgatatt
     1741 ctgccccagc actcagcagt tgcagcacca gaaaattacc gaaacgatcg atagtaatac
     1801 ccggcaggcc atcagattct ccggcgatta aacgatagct gtcgaggcca tctttttgcg
     1861 ccagtcagtc acgccatttt tgtgcttgtt gcaaacggcg ggaaaaaaaa gcaatgtcga
     1921 tagactcaga cgggtcaaac gtccagacgc gcgcccggat ttgcgaagct ggcgaataag
     1981 cgccgcgtgc taaccatttt ccctgatgat caacaatatc gatggtttca ccgaggctgg
     2041 ctttaccttc catgcgggca acggccccgg aaaagaccca cggatggcga cgaagtaatg
     2101 atttttcgcg ccctttggct aacactaaac gtacactcat aatttacttt tctgtcgatg
     2161 caaagaaatg ggcgtcattg tccggagttt tccatggatt tgcaacgcgt ccattaagga
     2221 taaggagagc agcaatgtcg aaagtctgca taattgcctg ggtttacggg cgagttcagg
     2281 gcgtaggatt tcgctacacc acacagtacg aagcgaaaag actgggttta accgggtacg
     2341 ccaaaaatct tgacgatggc agcgtggaag tggttgcctg cggtgaagaa gggcaggtgg
     2401 aaaaattaat gcagtggtta aagagtggcg gcccgcgttc tgcgcgtgta gaacgggtgc
     2461 tcagcgagcc gcatcatccc tcgggggaat taaccgattt tcgtatccgt taaatacatt
     2521 ttaccggttt tggcaggcca gcaattttgg tggcttgctt agccggacct ttcggaaaca
     2581 gtcggtataa atagcggctg ttaccttttt cttcgccaaa tttattcgcc atcgctttta
     2641 ccagcatacg aatcgccgga gaagtattga attccagata gaaatcacgc acaaaacgca
     2701 ccacttccca gtgttccggt gacagcgaaa tcccttcgtt ctctgcaatc accactgcca
     2761 gcggctcact ccactggctg ctttctttga gatagccttc ggtatccgtt tctatctctt
     2821 taccttcgaa gatcagcata attattacta ccttaatcag actggcggca gtgtaacaaa
     2881 aaacaaagcc ccgcataaag cgaggctatg aaagtgttag cgggtgagat taatcgcggc
     2941 tggcgaagcc cagaatgctc agcaggctga cgaagatgtt gtacagcgaa acatacaggc
     3001 taaccgtggc acgaatatag ttcgtttcac cgccatgaac gatgttgctg gtttcaaaca
     3061 aaatagcgcc agaggagatc agaatgaaga ccgcgctgat cgccagatgc agagcaggca
     3121 gctgcaggaa gatattagca accataccaa tcagcaccac cacaataccc gccatcagca
     3181 taccgccgag gaacgacata tctttgcggg tggtcagcac atatgcagag cagcagaaga
     3241 acactaacgc cgttccgccc agtgccatag cgattacgtc acccattccg gcagacagat
     3301 aggtgttcag aataggtccg aggatataac ccagaaaacc ggtaaaggcg aatgcggaga
     3361 taatcccggt cggcttattc gccgttttat aggtcaggaa catcaaacca tacataccca
     3421 ccagcgtcag aatcagaccc ggagatggca gcatcagcac cgtactggca gttgcggtaa
     3481 tcgccgaaaa ggccagcgtc aggctcagca gaaaataggt attacgcagc accttatggg
     3541 tgctaagcag tgatgtacgg tcatgtgaag aactaacaat acgatccatt gagtcactct
     3601 ctatgacaga tgtaattaat taagcagcat aatgataatg cgtaagggca cccagaagtt
     3661 ttacccatct ttacgcattt gatctggaac aggtttaaca gcggattatc aggtcattaa
     3721 gcaaatataa cgccctgaga atttcgacag gcaaaagaaa aaggggttag catttagcta
     3781 accccttatc ttatttggcg gaagcgcaga gattcgaact ctggaaccct ttcgggtcgc
     3841 cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga cgcgcactat
     3901 aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa aacagccaaa
     3961 agtgcattga tagcgtgaaa taacagcaga ttgatcattt catcaccatg aattccttct
     4021 cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggcaaaaag aaaaataggt
     4081 ccatttttat cgctaaaaga taaatccaca cagtttgtat tgttttgtgc aa
//
LOCUS       DAZSBF010000294         4124 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZSBF010000294 DAZSBF010000000
VERSION     DAZSBF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.299"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..83
                     /locus_tag="ACHXWD_003988"
     CDS             <1..83
                     /locus_tag="ACHXWD_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7622737.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="ACHXWD_003989"
     CDS             459..566
                     /locus_tag="ACHXWD_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7622738.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHXWD_003990"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="ACHXWD_003990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR7622739.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHXWD_003991"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="ACHXWD_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR7622740.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHXWD_003992"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="ACHXWD_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR7622741.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1051 a         1029 c         1074 g          970 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaagg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccacccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAZSBF010000295         4107 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZSBF010000295 DAZSBF010000000
VERSION     DAZSBF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.300"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHXWD_003993"
     CDS             complement(<1..759)
                     /gene="nlpA"
                     /locus_tag="ACHXWD_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR7622742.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
     gene            981..1274
                     /gene="yicS"
                     /locus_tag="ACHXWD_003994"
     CDS             981..1274
                     /gene="yicS"
                     /locus_tag="ACHXWD_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR7622743.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHXWD_003995"
                     /pseudo
     CDS             complement(1315..2495)
                     /gene="nepI"
                     /locus_tag="ACHXWD_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2706..3158)
                     /locus_tag="ACHXWD_003996"
     CDS             complement(2706..3158)
                     /locus_tag="ACHXWD_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR7622744.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHXWD_003997"
                     /gene_synonym="yicO"
     CDS             complement(3211..>4107)
                     /gene="adeQ"
                     /locus_tag="ACHXWD_003997"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR7622745.1"
                     /translation="LESLPGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGI
                     LGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSG
                     ALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAG
                     AFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALI
                     FVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRW
                     RDLNLCVVVVASLFALKIILVD"
BASE COUNT         1211 a         1080 c          911 g          905 t
ORIGIN      
        1 tgattgataa gattgcagaa attccttcac gttttctgca ttcttattat cttcccgtgc
       61 caccaaaata ttcacatacg gcgaattctt atcttcaata aatacgctgt cgtgcaccgg
      121 agaaagccct gtctgctgaa tgtaagtggt gctgataatc gctacatcaa ctttgggatc
      181 atccagtacg cgcgggagct gcgccccctc gagttccata atctgcaaat gacgaggatt
      241 atcggttata tccagcgcgg taggtaataa cccttttccc tctttcagag taattagctt
      301 ctctttttgc aacagtaaaa gtgcgcgacc aaggttggtc gggtcgttgg ggatcgccac
      361 tgtcgcgcct tcttttattt gcgccacggt tttgattttt ttggaataac ccgccatagg
      421 gaagacaaac gtatttccca gcgctaccag tttataacca tgcgcctgat tatcttgttc
      481 aagaaagggt cgatgctgga agacattcgc atcaagttcg ccatgatttg tcgcatcgtt
      541 cggtagcaac gaaccgctaa atcctaccag ttcaacatcg aggccatatt tctcttttgc
      601 caccttttta gcgacttccg cgacatcttg ttctgcgcca tttataacgc caactttaat
      661 gtgttttgca tcgctgctac tctggtcgca acctgccagc agaattccgg ccagcaataa
      721 tgcggcccct gtccgtagat gatgtgttgt cagtttcatt tttttatcct tttattaatg
      781 attacctgac tatagcggta aggtcgctgc ggtttaaaaa acgaaacgct atcgataaga
      841 ataaaaagga ataaaagtgg aatataaagt ctggcacttc ttactgacca cgcaagcgcg
      901 tttcgtacag catgatgaga gcgatgaaag caaactacac ttatgcttca ttcgatacac
      961 atttgtaaaa ggttgaatcc atgaaaccaa cgacactact gcttattttc acttttttcg
     1021 ctatgccagg catcgtatac gccgagtcgc cttttagctc actgcagtcc gcaaaagaga
     1081 aaaccacggt attacaagat ttacgtaaaa tttgcacgcc acaggcgtca ttatcagatg
     1141 aagcgtggga aaagttaatg ttgtcagatg aaaacaataa acaacatata cgcgaggcca
     1201 tcgtggcgat ggaacgtaat aatcagagca attattggga agcgttgggt aaggtagaat
     1261 gcccggatat gtaagaacag caacaactac tgaccaacgc ttctttacct gatatcagga
     1321 tttcttcatt ttcacctttg cagtaaccaa caatgcagtc agcaacatca atgtgccaga
     1381 aaacatcaac ggcgaagtca gaccaatatt atccagcgca taaccgccga ttgccgcgcc
     1441 acaagctgaa taaccgccac ctgaatagac ccggcttttt ctgcctgatc ggccagtgag
     1501 cgggtgatcc acgttgacca gccgacggga accaatgcaa aagttagccc ccagataatc
     1561 gccacgccgg tagcaacgat tttatcgctt ccccacaacg tcagcaccaa cgcactcaca
     1621 gccagtatta acggcgcgcc tgctaaggcc agttttaccg aacgtttaag aatgaatgac
     1681 gaaagcgacg taccgataaa gctggcaatg ccaaaactca acagcaccag cgttaagcca
     1741 tccacgctga atcccgccag gttcatatac accgggcgaa tatatgtgaa gaaagcaaac
     1801 tgcccggcga aagacatgaa gatggcgatc atccctgcca tcacacccgg acgttgtaat
     1861 aagcggaaag tattttgttt ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg
     1921 ataatccaga aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa
     1981 ccgataagct cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc
     2041 gcgccgaaga tcaccgacag cgccttcggc accgtacgcg gcggcaccag acgcatggtc
     2101 agcgacgccg acatcgccca gaacccgccc agcgccagcc ccagacaggc acgaccgatt
     2161 aaaagcaaac tgaatgagtt agcaaaggaa accagcaagc aggagagcgt cagcaaaacg
     2221 gcaaacaaaa taacaacgtt gcggcggtca gtagcctgaa ttgtctgggt aataaacaaa
     2281 ctggcaaaca ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa
     2341 atgcctaaat cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataatc
     2401 agacaggcga cacaaaacgc caccgagaaa acggctgacc agttcggtcg ggtgatggca
     2461 tccgcgccgc ggttttcggc aataaattca ctcatggtgt tacccgtggc atgtttcagg
     2521 ggaaaagccg accactttaa cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt
     2581 acggtgttgc ttgagctgga gttaagatcg aaacggaaca gggcaaagat taccggatgc
     2641 ggcgtgaacg tcttatccac ccgacaggtc aggcgcggtc tgtaggctga taagacgaag
     2701 cggttttaat gcagccagtg aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc
     2761 tgaaaagcat taatatcctg catttcaggt tcggtttcgc gcagaaaacc cagcgccagc
     2821 ctgacctgag catcggtaat cggcgcggaa aggcgggctt tttgtaaaat ccgatgccat
     2881 tgcagcaggt tctgttcgct accatcgaca atgacaattt gccagatgag caacacctgc
     2941 gcggcgtttt gcaggtgcga ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg
     3001 gctgactttc ccatttgatc gaccatcgac tccacgggta cgacatcgat attcagccga
     3061 tcactgagac ggcgaatcgc ttttctggcc ccggatgtca gcacccgaaa accaaccaca
     3121 aacactaccg ccagcgtggc gagcattatc cagatcatga gttctcctgc gttatcagaa
     3181 ctcatgatat ctggaaaccc tcgccagtta ctaatccacc agaataatct tcagtgcaaa
     3241 cagagatgcg accaccacca cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt
     3301 catgatgcag tacgacataa agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg
     3361 catcatcacc gtggtaataa acgcaggcac cgattcggta aaatcatccc agttaacgcg
     3421 cgccaggctc gaagtcatca gcacgccaac aaagattaac gctccggcgg ttgcgtaagg
     3481 aggaaccatc gccaccagcg gggagaagaa cataaccaac aggaacataa cgccaaccac
     3541 aaccgcagtc agccccgtgc ggccaccgac tgccacacca gaagtacttt caatataggc
     3601 agtaacagac gaggtgccga taaacgcacc cgccaccgaa ctgacgctat caacatacag
     3661 cgccttattc atattgggga atttaccgtt accatctatt aagcccgctt tatcagttac
     3721 accaattaat gttcctgatg aatcaaatag gttgatcagc ataaaggaga aaatgatacc
     3781 ggcgagttca agtgttaacg cgccgctcaa atctacttca ccaatgacgc cgctaatatc
     3841 aggcggaatg gaatagacgc cgctaaaatg aacatcaccg aaaaataatc cacagcaaga
     3901 cgtcaccaca atagaaacca gcaccgcggc atgaaaatga cgtgatgaca acacggttat
     3961 aataaaaaaa cctaaaatac ctaacaacac gccgtgagaa cttaaatcgc caatcatcac
     4021 cagcgtgtct ttattggcga caataacgcc agtatttttt aatcccatta aggcgataaa
     4081 taatccaatt ccgggtaatg actccaa
//
LOCUS       DAZSBF010000296         4076 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZSBF010000296 DAZSBF010000000
VERSION     DAZSBF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.301"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHXWD_003998"
     CDS             <1..784
                     /locus_tag="ACHXWD_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7622746.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1757
                     /locus_tag="ACHXWD_003999"
                     /pseudo
     CDS             797..1757
                     /locus_tag="ACHXWD_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="ACHXWD_004000"
     CDS             complement(1795..2694)
                     /gene="nhaR"
                     /locus_tag="ACHXWD_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR7622747.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="ACHXWD_004001"
     CDS             complement(2760..3926)
                     /gene="nhaA"
                     /locus_tag="ACHXWD_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR7622748.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1225 a          953 c          861 g         1037 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaatagag cttccaggaa ggtcatctta taatttagaa gaaatgctat
     1141 ctgcgataaa tttcaccatt ccatactcta tcgcatctgt atcagatagt tccaacaccg
     1201 cagatatttt tccaggaact ccttttcctt tgttgttcga aattgaagtg agttcatgta
     1261 cagggaataa tactaattgt aatagcgtcc gctttaatta taatttaaat actattttac
     1321 aggtaaattt aatgacctgt ggttttgaag atcaatccat cgacactgga cgatttgtac
     1381 tgtctcgtat caataatgaa aattatcagt ttcatcatat aagatttgac tgcatccagg
     1441 gcgagcaagg tgaattagtt tttgaaccca gtgatgttga atattacttt gaaccagtga
     1501 cgatacagga gtcaggaacc agcatattaa aaaatgagtt agaaaacagt gaggacggtg
     1561 cgggtcaagt aggctttcaa ccctccaatg atggtacgaa tgaaatccag tatggtaaaa
     1621 gcaattacta ctcttttcag catccccatg aaggaagtaa tcagattcct ctatttattc
     1681 gcccgcgtac atatggaaat aatgtctcaa gcgggcaaat aatgagccga gtcaaaatcg
     1741 tggttatgta caactaatct atctccccca ccttaacttt gggggaggtg ccgattaacg
     1801 cgccgctgga ctaaaaagcg cagaataatc cgtattgcag attcgctgta ccgccgggtg
     1861 ctgaatcatc cgctcagcaa aaatagcatg gtactcttcc atcacattct cgacgcgacc
     1921 aatttctacg acagttttat cggcataaaa gtcatatgca taaagcgttg gggcaacgaa
     1981 gattgcattg tgcatcgcac caaaagcttt catcaacgcg gcatcatcaa actcgccgag
     2041 gatttctacg tttaatccct gggagttaaa ccagttaagc aatttgcgcc ctaacattga
     2101 acgtcgccca ggaatcaaaa gtcgccgttc ttccagacaa gccgggaacg atttttctgg
     2161 tggtggattt gtacaccaga aactcacgcc acattcgcca attctcacgg agaacaggcc
     2221 ttcctgctgc gtagagtcta tcggacagtc agaaatgatc atatccagtt tatgctgact
     2281 taattgctcc agcagcattt cgtgggtgga ttcgaagcag cgaagatgaa tgggctcgcc
     2341 ttctaccact gcggcgttaa gtacgctact gaccaggcgt ttggaaagtg catcagccac
     2401 gccaacgtca aacaataaat tggattcttt gcgatagttc acaatatcca gcatttcctg
     2461 gcttaaggtg aacattttat cggcatagcg atagaccagt tctcccagct cgctgggttc
     2521 gagaccacgt cccttgcgtt taaataattt gccttgcagg cgctcttcca gcgctcgaat
     2581 ctgtccggta atggtttgtg gagttaaata aagcgcctcc gctgcgccaa ccacggaacc
     2641 ttctttatag acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat
     2701 ttctctccct gataacaatg aaaagggagc cgtttatggc tccccggtaa accgtcctgt
     2761 caaactgatg gacgcaaacg aacgcgtaac cagctgtatc caattaccgc cgaagagata
     2821 gaaccgacca ggataccgag tttcgcccag ttaatcagtt ctggatctac gctaccaaag
     2881 gccaggctgg caataaagat agacatagta aaaccgatac cgcacaggat ccccaccgcc
     2941 ataatttgct gataagtcgt tccctcaggc agatgcgcca gtttcaaacg cagcgccaac
     3001 cagcagaaca gactaatccc cagcggtttg ccaatcagca agccagcgat gatccccaat
     3061 ggcagaatgg aggtcaagcc atccagcgtg acgccttgca gtgaaacgcc agcattagca
     3121 aatgcaaaca gcggtaaaat cagatacgcc acccacgggt gcaatacatg ctccagtcgc
     3181 ttcgctggag aacgcccatg cttctctttc aaaggaataa agaagccgac aattaccccc
     3241 gccagggttg cgtgaacccc cgatttcaac accgcagtcc acaacaccac gccaacaaga
     3301 atatagacgc ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct
     3361 acagccgcga cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata
     3421 atgatggccc caagatcgtc gataatagcc agagccatca aaaagatctt cagtgctaac
     3481 ggaacacgac ttcccaacag cgccagcaca ccaagtgcaa aggcaatgtc agtagccgcc
     3541 gggatcgccc acccttcgcg ggtaatcgga tcggcatagt taaaggccag atagagtaat
     3601 gccggaacaa tcatcccacc aatagcggcg ataaccggaa atgcggcctg gcgtaagctg
     3661 gctagcgatc cttgcatcag ttcacgttta acttccagac cgactaacag gaaaaatacc
     3721 gccatcagcg cgtcatttat ccataacagc atgtttttgt tgatttcgag tgaaccaacc
     3781 cggagctgaa ccggcgtctc cagaaagtcg tgataccatc cactggttgc gccgctgttg
     3841 gccatcatca tcgccaggat agcggcaatg ataagaataa tgcctcccga ggcatcactg
     3901 ctaaagaatc gatgcagatg tttcactttt tatttctctt tcaggtgaat agatcgtttt
     3961 tactatttta ccccgcacaa atcatcgtta aaatcagatt atagtgtagg aataactcgt
     4021 tttttacgag caattaaagg atttgcgcca tcagcggtga gatgagtttt tgacgg
//
LOCUS       DAZSBF010000297         4062 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZSBF010000297 DAZSBF010000000
VERSION     DAZSBF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.302"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            245..367
                     /locus_tag="ACHXWD_004002"
                     /pseudo
     CDS             245..367
                     /locus_tag="ACHXWD_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="ACHXWD_004003"
     CDS             complement(629..1195)
                     /gene="ipgB2"
                     /locus_tag="ACHXWD_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFR7622749.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSREIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1478..1792)
                     /locus_tag="ACHXWD_004004"
     CDS             complement(1478..1792)
                     /locus_tag="ACHXWD_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622750.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(1870..1980)
                     /locus_tag="ACHXWD_004005"
                     /pseudo
     CDS             complement(1870..1980)
                     /locus_tag="ACHXWD_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="ACHXWD_004006"
     CDS             complement(2304..2981)
                     /gene="ospD1"
                     /locus_tag="ACHXWD_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFR7622751.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKSNIINVTNSAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3352..3489)
                     /locus_tag="ACHXWD_004007"
                     /pseudo
     CDS             complement(3352..3489)
                     /locus_tag="ACHXWD_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3568..3738)
                     /locus_tag="ACHXWD_004008"
     CDS             complement(3568..3738)
                     /locus_tag="ACHXWD_004008"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7622752.1"
                     /translation="MTHHLGCEKNQLRSGSNSRNGCLTKIITTGDEPLEIRTLRDRNG
                     TFEPQQLKKNQP"
BASE COUNT         1101 a          844 c          782 g         1335 t
ORIGIN      
        1 aaattcaggc gaatgctgtt tacgtgtttt tttgctggtt gatactgttt ttgtcatgtg
       61 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
      121 caatttgctc actggtattt actacgtgca tcgaatgagt gatcaagagc cgtatgacgc
      181 gagagtgtca cgtacggttc cgtgagcagc tggagagtga aactctctcc ggctgactcg
      241 actcgcaggt tccgacagcg gtgacgaaca tgcagcggtg ttgttctcgc tgatcggcac
      301 acgccgtctg aacaatgtgg agccagaaaa atggctgcgt tacgttattg aacatatcca
      361 ggactgaccg gcaaaccggg tacgcgatct gttgccttgg aaagttgatc tgacttctca
      421 gtaaatatca atatgattct gacgagccgc ttacctttga gcagagaaag gggagaggga
      481 tcccacatcc atgagctagg aaaatacaaa aactggctat acaacaattc aatatattag
      541 gacttacttg cgaggagaaa tatagcaccg ctcaggcaat tcctgggtat ttacttttct
      601 tagtttcttt actcgggatg ttctgtcatc agaaaggcga ttctaaattt gtaatatagt
      661 cactcatatt cccacgtaat ttctttagat caatatattg cacatttttc tcaaaaaact
      721 gatcggaaat aagatttgca acattgcttg ttgtatcatt tttaacgctt ctattcatcc
      781 cttcacaaag ttcgtccatt ttctttttga aataactgct tcctgataca agatgattta
      841 tggaactttg tgcagcattg atgtctagag aaatgcgata atctttctca acctgtttaa
      901 atatctgttt acggaggtta tcgccgattt ctcggctgtg ttctgcacaa aacttatcaa
      961 tacattggtt taccacccgg gatacgtaga cttttttttc atcatttttc caatctgtaa
     1021 tattttcacc gatttgttct gaaacagagc tcaaaatact agtatttatt ttggttgatg
     1081 atatttttac tatccttttc cccattgtac agcggataat ctcatccacc tttcctgaaa
     1141 aatatctttt gtgagataaa ctgattccaa aattattaaa agatgttcca agcattataa
     1201 tcactttgtc atgtaaaaat agtaacattg tcattatttt agctgattca aacaattctg
     1261 taaatgcttt gagttcttta ctcccttggc tcaatgatgg accgtctgga atcccagaga
     1321 ctaaactctc tgagaaagag agtatgaaaa aacttggggc agtccctaaa tgaagggacc
     1381 aacagcggta aaataaccgg catcattcat aatgatgccc tgttagttgt agttatgaag
     1441 atttacgtca ttaaaattgc tgttcgtagt gtcagtccta tggtctggca tcagctgagg
     1501 atcgtagccg atacatcact ggcttcacga cattcgtgtt gaggctattc atgaaaactc
     1561 cacgctgaaa acgccgtttt gtataaacgg tcacggtatg cccggagcca ctgttgctga
     1621 tgaattcgat aaaaccttgg cgtttcttga agctatcgtt aatgcggata atgaaacaac
     1681 gattggtgag attcgctctt ttgccgacgc cctggacgct gtccggttca atcgcaacaa
     1741 aatcaaccgg cagctgagca aacccaacct tgcctctctg gcgctggagc atgaagtgat
     1801 ttggctggga cgtagccgct gacagcttct ataaaagtat gatcctaccc acgtaatatg
     1861 aacacaggct taagtgaggt tctggttttc aaattgttca ggactgagac ctccacacca
     1921 actgtgccgt cgtcagcgat tgtaatcgca cttgatataa ttaaacactg tatatttcat
     1981 ttgtggagtc tccagtctga gggggcttta tgcattccgt attgggcact atgatgccag
     2041 aaatctgcga ggctccacac cttcctgtat ttcccttcct ctatcacagt gcactttact
     2101 taatagggtg ggatgtgttc attacattta ttgtgttttt tatgattgtt tttgcgtcca
     2161 gtgtcgttcg tcatgggaaa ctggttctat gggtagtatc gctcagtcta gttaactgtt
     2221 tgtttctttt tagaaattaa gcagatcaca caattcgggg agagatcctt ctaagtgcaa
     2281 ttctttgcag ctatttagca cacttaaaac aactcttgta ttttgtcaga aataatatta
     2341 aacatgtctt gttcattatt gttaagtctg tctgctattt ctgtaagttt tatcccacta
     2401 tgatggcata tatttatata tgccatcata gcctcgatat tccctttccc tgcggccaga
     2461 aataatcctg gtaaaccctc attatctctt acatccaata gtttaatcgt ttcttttggt
     2521 gataaatcaa aagttttaat aaaatctcca taagcttgaa tcgcagcgga atgaccttct
     2581 tgaagagcta tgaacaaagc agattcccca gcattatttc gtgctgcaag aacttctagt
     2641 ttttcctctt tgggtatttc ggctgccaac agcgattcac cgtatttacg cgctgcatcg
     2701 gcatttccat gccaaatcaa tgaaaacaaa ttatggcggt tatactcctt attattcttt
     2761 gtcggaatgg atatgctctg tattttggcc aattttctga aagaaacacc actatatccc
     2821 ccccctgatt tttcttcatt gatggcgtgg gatatagcgg aatttgtcac gttaatgata
     2881 ttacttttca ttcctttatt ttcattagca gtattgctgc ctattattag gtgcatattt
     2941 ttgttgtttg ctggatgtaa tccatagtta tttattgaca tttttgttca tcctttttgc
     3001 taaaaaaaac aataacctat ttttgtaatg taatagatat tagtctattg ccaatctaaa
     3061 aaaaccattg ccacattgaa tggctatatt caccaataaa ttctttcgat ttcgtatatt
     3121 gacatatcgt agatatatct tatccctaag attcaataat cgagtccgtt taccattctg
     3181 tgcaagcgcc ttcactcaga agtgaccgtc aaggcgatca ccaaacttga taataaagca
     3241 gctcattgcc atacgccagt ccttaagggg cgttgtccat ttctgtgatg ctgcctagat
     3301 ttaactgcca gctccggctt atctacatct aaatatgtga gcctaatacc gctacacttt
     3361 ggccagacta tgtttttctc cgggaagtgg gctaaccgtc tccataaagt gacgaacttt
     3421 tttcaacagt ggccatattg aacggcactg atgattacgg gttattgtct catgaagagc
     3481 cggccacatc actgacagca gtgacttcag gccttcagca tcagacagcc tgaaccagtt
     3541 aatccatcgc ccgacagatt tagtaatcta gggctggttt ttcttcagct gttgcggttc
     3601 gaaagtgcca ttacgatcac gcagagtacg gatttccaga ggttcatctc cagtagtgat
     3661 aatcttcgtt aagcaaccat tgcgggaatt gctgccagat ctaagctgat ttttctcgca
     3721 cccaagatga tgagtcatat tagcattgag tacggtttca ttttcagcat ccaatcaaac
     3781 tgactgagat cttcaggatt tttgagactt ttggctagtt cattagctaa agcccgcaac
     3841 ttattagatg tcaactgttt ttcattcata aattcacctt catttgatat caaattaaat
     3901 agttcaacat cagagaatta tacaaactta tgtacaggct cctatgatct tcgaatcctg
     3961 aagattatag tgaaaataag ttttcacgtt gaaatcccac atagttagta tatgataaat
     4021 aatcatggat gacctgatta acctcttggg gtagtgcttt ta
//
LOCUS       DAZSBF010000298         4059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZSBF010000298 DAZSBF010000000
VERSION     DAZSBF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.303"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(112..1431)
                     /locus_tag="ACHXWD_004009"
     CDS             complement(112..1431)
                     /locus_tag="ACHXWD_004009"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR7622753.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(1562..3097)
                     /gene="gadC"
                     /locus_tag="ACHXWD_004010"
     CDS             complement(1562..3097)
                     /gene="gadC"
                     /locus_tag="ACHXWD_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR7622754.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(3253..>4059)
                     /locus_tag="ACHXWD_004011"
     CDS             complement(3253..>4059)
                     /locus_tag="ACHXWD_004011"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR7622755.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1042 a         1021 c         1038 g          958 t
ORIGIN      
        1 aattgcgtgg ttaatttgac ttatgtaagc atgattacta gtgggatatt tcacaaggtg
       61 taacaagccg gtgggtaaag caccggcttg ttacaaagta agcatgggag tttaactgcc
      121 ccaacgactt tgcagatagc tgaccgcttg ttgagtctgc ggtttattca gatagtcctc
      181 acggaacaag atggtgccgc taatttctgg taccgcatcg ttaagatcga gctgcttttt
      241 cagttccggt acgccgccgt taatcatcca gtctggctct atctttgaag gttcacccac
      301 tttatagaag gcgataccga tatacaggcg ggtcctagtc ggtttaacga catccgccca
      361 ccattttgcc aacacgtcat aacgcgcggc actccgtgag aacggccagt aaatttgggg
      421 agcaatgtaa tccagcaatc cttgttccac ccatcgacgg gtgtcagcgt aggattcgtc
      481 ataggctgcc gcgcctcggg tatcggaacc gagcggatcg tgtgatcggt tacgccacac
      541 gcctgccggg ctaacgccaa attcgactcc cggcttaatg cttttaatgg tgtgcgatac
      601 ttttgcaatt aactgctgag tattgttgcg ccgccagtct gcttttgacg caaatgcgcc
      661 tccgtatttg cggtacgttt cgttatcatt tagccgtgaa cccggtgact cagtatagaa
      721 atagtcgtca aactgcacgc catctaccgg atagcgggaa accacttctg cgactattga
      781 tgtgatccag tcctgaacct cagggatgcc cgggtcgagg acaaagcgat cgccagacgt
      841 tctgatccag tcacggtgtt gcacatagac gctcgccggt tgttgagaca gagtgctatt
      901 cagttccctg atagtaccgg gcttcgtatt aaccgatacg cgataggggt taaaccaggc
      961 gtgtactttc atcccacgct tgtgggcttc gtcgagcata aattgcagcg gatcgtaacc
     1021 cggattttca ccaatcttac cggtcataag atcggaccat ggcaaaattt tcgatggcca
     1081 cagggcggta ccgtccggct taacctggaa aaagaccgtg tttataccga gacgttgcag
     1141 atgatccagt ttgtcgatca tcgcctgttg ttgtacacgg gcccggctgg tggggttact
     1201 aatgttaacc gaggaaaccg gtggccagtc gaggcgagac accgtggcca gccagatgcc
     1261 acgcatcggt tgtgacgatt gttgcgtcgt ggctggtggc tttgaacctg ctggtggtgt
     1321 caccatggac tctggaggcg tgcttttaca actacacagt aaaagtgcca gtgcaaccag
     1381 tatcgctggt cttctaatcg ctaatttctc gtttcgggag cagatatcca tatatgctgg
     1441 tttccggtaa acagatgtgc gctcattctc gtacttatcc cggttaagtc aatacgacag
     1501 cgagcacgaa aaagggagcg atgaattatc gctcccttgt cttataacca ttcagacatg
     1561 gttagtgttt cttgtcattc atcacaatat agtgtggtga acgtgcacgc gggtgcagga
     1621 agaagtgacc ttttggtgcg ttctgactgt tgattggctc cagagtgacg ccggtattcg
     1681 ctttgccttt acgatcatga acagcataga gaataaaggg cagggcaagt accaccagga
     1741 aactaacgac cagtaattca acatacatat cggtagagtc accctggatg ttatccggcg
     1801 gcaggaagga aacaataaac gccataattg aagtcagcag accgacaata gccacgacca
     1861 gtttcacccc tttaccacca gggatattaa atgtgcgttt taagtcagga tgtttaagaa
     1921 ccaacacaat gtagccaata aacagcatga aataagcaca cagataaatc accaccgtca
     1981 gcgccagtgc gatcaggaag gacatgttgt taccgccacc ggtattggtg aggatgatca
     2041 acgcgataga agtaatcacc agctgtgaaa tgaccagcgt taccggtacg ccatttttgt
     2101 tcattttagc gaatgccgct ggcagcaggt ttttctgcgc tgtgacatac atcccgcgag
     2161 aaggaccaac aatccaggag gcgatttccg ccagaacacc cagcaacagc agtgcggaga
     2221 tcacgcgaac cgtccactca atttccggtg ccacatggga catcagaacg gtaaaggttt
     2281 gcattacccc tgcggagagg ttgatttcat tacccggaat gaccatcgca atagacaaac
     2341 caccaacaga gcttaagcag attgccgcta ccatcagcag taacatagcc aacggatagt
     2401 cgcgccctgg gttgctcatt tcattgacgt gggttgcgga tgcttctacg cccatataac
     2461 tcaaaatgaa ggcaacaaat acaaccaggg tgcccacttt agagaagtca gggaagaagg
     2521 tctttgaatc catttcgata gcaacggggg caccggagtg cagataaata gccgctaatg
     2581 cgatcaaaat aaatgcaggt aacaggatac cggcgaagaa gccaacttta gcaattcgtg
     2641 ccgtgtattt cgtgccacca aactgcgtta atgccagcgc ccaaagaatg atgagtgctg
     2701 caatagtttt ggtaatgggg tcttcattca gcgctggcca tttcaggatg taggagagtg
     2761 cccctaacac gaaatagagc atcggaataa aaccaatggc gatttgcaga tagccaaatg
     2821 agatcgctgc aaatccccat ctcggcccca gagtatttga tacccaggcg aagacaccac
     2881 cttcttccca gccgtcgacg gtggccattt ccgcagcaca aagtcccacg ggaataaacc
     2941 ataaaatccc gcctaatagc aggaagaaga ctaatgaaaa gcccgatgtt gcgaaggtag
     3001 ggtattcata aacagccatt accatcgatg ccgttatggc aaaaaagccg agtaatgtga
     3061 gctgcttagc tttacctgtc tgtactgatg tagccatatt atccccctaa aacggtattc
     3121 ctgtcggaac cgcacctgtt ttgttctgaa gcgtattcag aacaatattt tccgttgcta
     3181 atgccagtga acagactttg gaaattatcc cgaaacgggt tcgtttcggg acaccgttac
     3241 cgttaaacgt tatcaggtat gtttaaagct gttctgctgg gcaataccct gcagtttcgg
     3301 gtgatcgctg agatatttca gggaggcttt gtagtcttcc agcaacagtt cagcaaagtc
     3361 catttcgaag ccgcgacgac acataatgcg catcaccacg atgtcggtgg cttcaccgcc
     3421 gagagtgaag gccggaacct gccagccgcg cagacgcaga cgttcagaga ggtcgtacag
     3481 ggtgtatccc ggatcttcac cctctttcag tttgaagcaa accgccggga tgccttcgtc
     3541 cgggcgaccg gcacagatga actcatacgg ccccagtttg gcgatctcat ccgccagata
     3601 ggcagcgacc tggtaggaag cattctgtac tttggtatag ccttcacgac caaggcgcag
     3661 gaattcatag tactgtgcaa tcacctgacc cgccgggcgg gagaagttga tggcaaaagt
     3721 accaatctga ccaccgaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc
     3781 gtcacgccag ataacccagc cgcagcccag cggagccaga ccgaatttgt ggcctgaagc
     3841 actgatcgat ttcacacgcg gcaggcggaa gtcccagacg atatccgggg cgacgaacgg
     3901 tgccaggaag ccaccgctgg cggcgtcgat gtgcatgtcg atgtcgatac cggtgtcggc
     3961 ctggaattta tccagtgcat cgtgcagcgg ttgcgggaac tcatagttac cggtgtaggt
     4021 cacgccgaaa gtcggcacca cgccgatggt attttcgtc
//
LOCUS       DAZSBF010000299         4049 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZSBF010000299 DAZSBF010000000
VERSION     DAZSBF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.304"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHXWD_004012"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHXWD_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR7622756.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHXWD_004013"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHXWD_004013"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR7622757.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHXWD_004014"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHXWD_004014"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR7622758.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHXWD_004015"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHXWD_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR7622759.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1143 c          971 g          965 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccgat
//
LOCUS       DAZSBF010000300         4047 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZSBF010000300 DAZSBF010000000
VERSION     DAZSBF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.305"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            42..1556
                     /gene="caiT"
                     /locus_tag="ACHXWD_004016"
                     /pseudo
     CDS             42..1556
                     /gene="caiT"
                     /locus_tag="ACHXWD_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            1587..2729
                     /gene="caiA"
                     /locus_tag="ACHXWD_004017"
     CDS             1587..2729
                     /gene="caiA"
                     /locus_tag="ACHXWD_004017"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR7622760.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2858..4047
                     /gene="caiB"
                     /locus_tag="ACHXWD_004018"
                     /pseudo
     CDS             2858..4047
                     /gene="caiB"
                     /locus_tag="ACHXWD_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT          946 a         1033 c         1084 g          984 t
ORIGIN      
        1 gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa
       61 aaacgggaat agaaccgaag gttttctttc cgccgttaat aatcgtcggc atactttgtt
      121 ggcttacagt cagagatctg gatgcagcga atgtcgttat taatgctgta ttcagttacg
      181 tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt
      241 tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaacca cctgaattta
      301 gtaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct
      361 ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact
      421 cgacaggggc gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt
      481 gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg
      541 aagtgattcg ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt
      601 tgttcggcac tatcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca
      661 gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc
      721 ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg
      781 tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct
      841 tcctgatgct gggttgggtg ttcattgtca gcggtgccag cttcatcatg aactacttca
      901 ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat gttgttctat accgatccca
      961 tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt
     1021 atgctatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac
     1081 tgtgcttcgg catggtgctg gggctgacag cgtcaacctg gatcctgtgg actgtactcg
     1141 gtagtaacac tctgctgttg atagataaaa acatcatcaa cattccaaat ctgatcgaac
     1201 agtacggtgt ggcgcgcgcc atcattgaaa cctaggccgc tctgccactc agcaccgcca
     1261 ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacactggtt aacgcctgct
     1321 cttataccct ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa ccacctctgc
     1381 tggtgcgtat cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg
     1441 gcggcctgaa accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca
     1501 acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa
     1561 cccccaaaat atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt
     1621 ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt
     1681 gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg
     1741 acagtctgct gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg
     1801 ccgtgtggat ggagctggga cgtctggggg caccaaccta tgtgctgtac cagttgccgg
     1861 gcgggttcaa caccttcctg cgcgaaggca cacaagagca gatcgacaaa attatggctt
     1921 tccgcggcac cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg
     1981 acgtgggtag cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta
     2041 gtaagtgttt tattaccagc agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg
     2101 gggcttctcc ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca
     2161 tcaaagtgac caaacttgaa aagctcggtc tgcgtatgga tagctgctgt gaaatcacct
     2221 ttgacgacgt ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc
     2281 gcgtcaaaga agagttcgac catgaacgtt tcctggtagc cctcaccaac tacggtacgg
     2341 cgatgtgcgc ttttgaagat gcggcgcgct acgccaatca gcgcgtgcag tttggcgagg
     2401 ctattggtcg tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact
     2461 ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg
     2521 gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtt gtggatagcg
     2581 caatgcaggt gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc
     2641 gtgatctgcg tgtagaccgc gtctccgggg gatctgacga aatgcagatc ctgacgctgg
     2701 gtcgcgcggt gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctaatgc
     2761 gtcttatcag gcctacggga gcacgaatgt aggccggata aagcgtttac gccgcatccg
     2821 gcagtcatgc actacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa
     2881 attcgggccg ttggccggat tgcgcgttgt cttctccggt atcgaaatcg ccgggccgtt
     2941 cgccgggcaa atgttcgcag aatggggtgc ggaagttatc tggatcgaga acgtcgccta
     3001 ggccgacacc attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc
     3061 gctgtcgtta aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac
     3121 caccgatatc ttcatcgaag ccagcaaagg tccggccttt gcccgtcgtg gcattaccga
     3181 tgaagtactg tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca
     3241 gtacggcacc gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag
     3301 tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc
     3361 cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg
     3421 tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg
     3481 ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg
     3541 taaagatccc tactacgccg gttgcggtct gtataaatgc gccgacggct acatcgtgat
     3601 ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattgacc tcgcacatct
     3661 actcggtacg ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta
     3721 cggcccactg gtagaagaaa aactcgatgc ctggctggcg gcacatacca tcgcagaagt
     3781 taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact
     3841 ggaaagcaat ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg
     3901 tcgcacctgc aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg
     3961 gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag
     4021 cgaaaacgac attcaggagt tggtcag
//
LOCUS       DAZSBF010000301         4045 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZSBF010000301 DAZSBF010000000
VERSION     DAZSBF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.306"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            152..3460
                     /gene="icsA"
                     /locus_tag="ACHXWD_004019"
                     /gene_synonym="virG"
     CDS             152..3460
                     /gene="icsA"
                     /locus_tag="ACHXWD_004019"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFR7622761.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGNGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVVGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQNEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            3604..3922
                     /locus_tag="ACHXWD_004020"
                     /pseudo
     CDS             3604..3922
                     /locus_tag="ACHXWD_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842854.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1219 a          698 c          956 g         1172 t
ORIGIN      
        1 gtaaatttct cccgttgcat tgatatataa cacagctctc atgttttggt tgaggctttg
       61 tttaatatat ttttgcatat atcgtccctt tattccggat aaaacggaat ctttttaggg
      121 gtttatcaac cacttactga taatatagtg catgaatcaa attcacaaat ttttttgtaa
      181 tatgacccaa tgttcacagg ggggggccgg agaattacct acggtaaagg aaaaaacatg
      241 caaattgtct ttttctcctt ttgttgttgg tgcatccctg ttgctcgggg ggccaatagc
      301 ttttgctatt cctctttcgg gtactcaaga acttcatttt tcagaggaca attatgaaaa
      361 attattaaca cctgttgatg gactttctcc cttgggagct ggtgaagatg gaatggatgc
      421 gtggtatata acttcttcca acccctctca tgcatctaga actaagctac ggattaactc
      481 tgatattatg attagcgcag gtcatggtgg tgctggtgat aataatgatg gtaatagttg
      541 tggcggtaat ggtggtgact ctattaccgg atctgacttg tctataatca atcaaggcat
      601 gattcttggt ggtaacggcg gtagcggtgc tgaccataac ggtgatggtg gtgaggctgt
      661 tacaggagac aatctgttta taataaatgg agaaattatt tcaggtggac atggtggcga
      721 tagttatagt gatagtgatg gggggaatgg aggtgatgcc gtcacaggag tcaatctacc
      781 cataatcaac aaagggacta tttccggtgg taatggaggt aacaattatg gtgagggtga
      841 tggcggtaat ggaggtgatg ccatcacagg aagcagcctc tctgtaatca ataagggcac
      901 gttcgctgga ggcaacggag gtgctgctta cggttatggt tatgatggct acggtggtaa
      961 tgctatcaca ggagataacc tgtctataat caacaatgga gctattttag gcggtaatgg
     1021 tggacattgg ggggatgcta taaatggtag caatatgacc attgctaata gcggatatat
     1081 aatttcaggt aaagaagatg atggaacaca aaatgtagta ggtaatgcta tccacatcac
     1141 tggtggaaac aattcattaa tactccatga aggttctgtc attactggtg atgtacaggt
     1201 taacaattca tccattctga aaattatcaa caatgattac actgggacca caccaactat
     1261 tgaaggtgat ttatgtgctg gtgattgtac aactgtttca ctatcaggta acaaattcac
     1321 tgtttcaggt gacgtttctt ttggtgagaa cagttcttta aatttagctg gaatcagtag
     1381 tctggaagct tctggaaata tgtcatttgg caacaatgta aaagtggaag ctattataaa
     1441 taactgggcg cagaaggact ataaactgct aagtgcagat aaagggataa caggtttcag
     1501 tgtttctaat atatctatca tcaatccgtt actcactact ggtgctattg actatacaaa
     1561 aagctatatc agtgaccaga ataaattgat ctacggtttg agctggaatg atacagatgg
     1621 cgacagtcat ggagagttca atctgaaaga aaacgctgaa cttactgtta gtactattct
     1681 ggcagataat ctcagccatc ataatataaa tagctgggac ggaaaatccc taacaaaatc
     1741 aggggaggga actctcattt tggcggaaaa aaatacctac tctggtttca ccaacatcaa
     1801 tgcaggcatt ctaaaaatgg ggacagttga agctatgaca cgtaccgctg gtgttattgt
     1861 taataaaggt gctaccttga atttttcagg catgaaccaa actgttaaca ctttattaaa
     1921 tagtgggact gtgctaatca ataatattaa tgcccctttt ttgcctgacc ccgtcattgt
     1981 cacaggtaac atgactctgg agaaaaacgg tcatgttatt ctcaataata gttcgtcaaa
     2041 tgtcggtcag acctatgttc agaaaggtaa ttggcatgga aagggcggaa tattatcttt
     2101 gggcgcggtt ctcggcaatg acaacagtaa aactgaccgg ctggaaattg caggccatgc
     2161 gtctggtatt acctatgttg cagtgacaaa tgagggaggc tctggagata aaactcttga
     2221 aggtgttcaa attatttcga cagattcttc tgataagaat gcttttattc agaaaggccg
     2281 tattgttgct ggtagttatg actatcgcct gaaacagggc actgtatctg gactgaatac
     2341 caataagtgg tatctaacta gtcagatgga taatcaagaa tcaaaacaga tgagcaatca
     2401 agagtctact caaatgagta gtcgccgagc tagttcacag cttgtatctt cacttaattt
     2461 gggtgaaggt agtattcaca catggcgccc tgaagctggc agttatattg ctaacctgat
     2521 agcaatgaac acgatgttta gtccttctct ctatgaccga cacggtagca ctattgttga
     2581 tcctactaca ggtcagctca gcgaaaccac catgtggatt cgtactgttg gtggacataa
     2641 tgagcataat ttagctgata gacaattaaa aaccacagct aacaggatgg tttatcagat
     2701 tggtggagat attttgaaga caaacttcac tgatcatgat ggcttgcatg tgggtattat
     2761 gggagcttat ggatatcagg atagcaaaac tcataataag tatactagtt atagttcacg
     2821 aggaactgtg agcggttata ctgccggttt gtacagttct tggtttcaga atgaaaaaga
     2881 acgaacaggt ctatatatgg atgcttggtt gcagtacggt tggtttaata atacagtcaa
     2941 aggagatggg ttaactggtg agaaatattc cagcaaagga ataacaggag ctttggaagc
     3001 tggctatatc tacccaacca tacgctggac tgctcataat aatattgaca acgcattgta
     3061 tctcaatcca caagtccaga taactaggca tggggtaaaa gcaaacgact atattgaaca
     3121 caatggcact atggtcacat cctctggggt caataatatt caagcaaaat tgggattgcg
     3181 tacatcctta attagtcaga gttgtatcga taaggagact cttcgtaagt tcgaaccatt
     3241 tttggaagtg aattggaaat ggagctcaaa gcaatatggt gtaattatga atggcatgtc
     3301 aaatcaccag ataggcaacc gtaatgtgat tgaactcaaa actggtgtgg gggggcgtct
     3361 tgcagataac ctaagcatct ggggaaacgt atctcagcaa ttgggtaata acagttacag
     3421 agacacccaa ggtattttgg gtgtgaaata taccttctga taataaaaat gcagggcgtg
     3481 ttgatgtcct gcatttctct gggacccacc gtgtaaaagc tcggtgggtt tttgtgcaca
     3541 tgatggtatg cctgcgatca atgccaaacg tgtttaccag atcatgcgcc agaatgcgct
     3601 gttgcttggc gttacacagg aattgcatta gaaagcgatg aacgaatgct accgccaaat
     3661 attcgtggtt atgcgcctca gattactggt attgcagaaa ctaatgcacg tgttgttgta
     3721 tctcaacaag gtcgagtaat atatgactct actgtaccag ctggaacttt ttctattcag
     3781 gacttgagta gttctgtacg aggaatattg gatgttgaaa tttttgagca aaatggtaaa
     3841 agaaaacatt tccaagtaga aatgtgcagg tgtgcctttc ttatccagac ttggtcagag
     3901 taggtataaa ttagtaacag gtctacctaa aacaaataat aaagcaactg gtgatgcatt
     3961 tttttcggtg aagatcctac ggggtcctga gcccggtgaa gtggcgaggc aaattacatg
     4021 gggaagcgta cagcctgaac tgaac
//
LOCUS       DAZSBF010000302         4043 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZSBF010000302 DAZSBF010000000
VERSION     DAZSBF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.307"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            79..687
                     /locus_tag="ACHXWD_004021"
     CDS             79..687
                     /locus_tag="ACHXWD_004021"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR7622762.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            785..2176
                     /gene="selA"
                     /locus_tag="ACHXWD_004022"
     CDS             785..2176
                     /gene="selA"
                     /locus_tag="ACHXWD_004022"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR7622763.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2173..4017
                     /gene="selB"
                     /locus_tag="ACHXWD_004023"
     CDS             2173..4017
                     /gene="selB"
                     /locus_tag="ACHXWD_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR7622764.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          899 a         1031 c         1203 g          910 t
ORIGIN      
        1 aacaaaccat caacattttc tgcccttttt cccatcacca ggccatactg atatttttcc
       61 tcgcacaaag gactcggcat gaaactcgtc ggtagctaca ccagcccgtt tgtacgcaaa
      121 ctttctattc tgttgttaga aaagggcata actttcgaat ttattaatga actgccctat
      181 aacgcggaca acggcgtggc gcaatttaac ccgttaggaa aagtgccggt gctggtgacc
      241 gaagagggcg aatgctggtt tgattcgccg atcatcgctg aatatattga gttaatgaat
      301 gtcgctccgg cgatgttgcc gcgcgatccg ctggagtcgt tgcgggtgcg caaaattgag
      361 gcgctggcgg atggcattat ggatgccggg ctggtatcgg tgcgtgaaca ggcgcgtccg
      421 gcggcgcagc agtctgaaga tgaattgtta cgccagcggg agaaaatcaa ccgcagtctg
      481 gatgtgctgg aaggatatct ggtcgatggc acactcaaaa ccgatacggt caatctggcg
      541 actatcgcca ttgcctgtgc tgtcggatat ctcaatttcc gccgcgttgc gccaggttgg
      601 tgtgtcgacc gcccacactt agtcaaactg gtcgaaaacc tgtttagccg cgaaagtttt
      661 gctcgcaccg aaccgccaaa ggcttgatgc gcgatatgtc ctcctgaccc atctcacgtt
      721 acaatccgtg gttatgttaa acgcccttct ccgtgtgaga gggccttgat cagccaggtt
      781 tcctatgaca accgaaacgc gttccctcta tagtcaactt ccggctattg atcgcttatt
      841 gcgcgatagc tccttccttt ctttgcgtga tacttatggt cacacccgcg tggtggaatt
      901 gttgcgtcag atgctcgacg aagcgcgaga agtgattcgt gacagccaga cgcttcctgc
      961 gtggtgtgaa aactgggcgc aagaagtcga tgcccggttg acgaaagagg cgcagagcgc
     1021 gctgcgtccg gtgatcaacc tgacgggaac cgtgctgcat accaaccttg gacgagcttt
     1081 acaggcggaa gccgcggtgg aagccgttgc gcaggctatg cgttcgccag tgaccctcga
     1141 gtacgatctg gacgacgccg gacgcggaca tcgcgatcgg gcgctggcgc aactgctgtg
     1201 ccgtattacg ggggcggaag atgcctgtat cgtcaataac aatgcggcgg cggtgttatt
     1261 gatgttggcg gccactgcca gcgggaaaga ggtggtagta tctcgcggcg aattggtgga
     1321 gattggcggc gcgtttcgta ttcccgatgt tatgcgtcag gcaggctgca ccctacacga
     1381 agtagggacc accaaccgca cgcacgcgaa tgattatcgt caggcggtga atgaaaatac
     1441 cgcactgttg atgaaagtac ataccagtaa ctacagcatt caggggttca ccaaagcgat
     1501 agatgaagcg gaactggtgg cgctcggcaa agagctggat gttcccgtag tgactgattt
     1561 aggcagtggc tcgctggtcg atcttagcca gtacggtttg ccgaaagagc caatgccgca
     1621 ggagttgatt gcggcgggcg tcagtctggt gagcttctcc ggcgacaagt tgttaggcgg
     1681 gccgcaggca gggattatcg ttggtaaaaa agagatgatc gcccgcctgc aaagccaccc
     1741 gctgaagcgt gcattacgcg cggataaaat gaccctcgcg gcgctggaag ccacgttgcg
     1801 tctttattta caccctgaag ctctgagtga aaaattaccg accctgcgcc tgcttacccg
     1861 cagcgcagag gtcattcaaa tccaggcaca acgtttacag gctccccttg ccgcacatta
     1921 cggcgcggag tttgcggtac aggttatgcc atgtctttcg cagattggca gtggttcgct
     1981 gccggttgat cgcctgccga gcgcggcatt aacgtttaca ccccatgatg gacgcggtag
     2041 ccaccttgag tcattagccg cccgctggcg tgaattgcca gtgccgatga ttggtcgtat
     2101 ttatgacgga cgattgtggc tggatttacg ctgccttgaa gatgagcaac ggtttttgga
     2161 gatgttgttg aaatgattat tgcgactgcc ggacacgttg accacggcaa aacaacctta
     2221 ttgcaggtga ttactggcgt aaatgctgac cgtctgccgg aagaaaaaaa gcgcggcatg
     2281 accatcgatc tcggctatgc ctactggccg cagccggatg gtcgcgtgcc tagttttatc
     2341 gacgtacccg gtcatgaaaa gtttctttcc aacatgctgg cgggcgttgg tggtatcgat
     2401 cacgcgctgt tggtggtggc gtgcgatgac ggcgtgatgg cgcagacccg tgagcatctg
     2461 gcgattttgc agctgaccgg taacccgatg ctgacagtgg cgctgaccaa agccgatcgc
     2521 gtggacgaag cgcgtgttga tgaggttgaa cgccaggtaa aggaggtgct gcgggaatac
     2581 ggttttgctg aggcaaaact gtttatcacc gcagcaaccg aaggtcgggg aattgatgcc
     2641 ctgcgcgagc atctgcttca gttaccagga cgtgaacacg ccagccaaca tagtgtccgc
     2701 ctcgcgatcg accgcgcatt taccgtaaaa ggtgccggtc tggtcgtcac cggtacggcg
     2761 ttaagcgggg aagtgaaggt aggcgattca ctctggctga ctggtgtaaa taaaccgatg
     2821 cgtgtacgtg cgctgcatgc acaaaaccag ccaacagaaa ccgcccatgc cgggcagcgt
     2881 atcgcgctta acatcgcggg tgatgcggaa aaagagcaga ttaaccgtgg cgactggctg
     2941 cttgccgatg cgccgccaga gccgttcaca cgggtgattg tcgagcttca aacccatacg
     3001 ccgctgaccc agtggcagcc gctgcatatt caccacgccg ccagccacgt tacgggacgc
     3061 gtttcgctgc tggaagataa ccttgccgag ctggtgttcg acacgccgct gtggctggcg
     3121 gataacgatc gcttagtgct gcgcgatatc tcggcccgca acacgctggc gggggcgcgt
     3181 gtggtgatgc ttaacccgcc gcgtcgcggt aaacgtaagc cggaatatct gcaatggctg
     3241 gcgtctcttg cacgggcgca gagcgatgcc gatgcgttat ctgttcatct ggaacgcggc
     3301 gcggttaacc ttgcggattt cgcctgggcg cgccagctca acggcgaagg gatgcgcgaa
     3361 ttgctgcaac agcctggcta tattcaggct ggttatagct tgttgaatgc gccggttgcc
     3421 gcccgctggc agcggaaaat tctcgacaca ttagcgactt atcatgagca acatcgcgat
     3481 gaacctggtc ctgggcgcga acgtctgcga cgtatggcgt tgccaatgga agatgaagcg
     3541 ctggtactgt tgctgattga aaagatgcgc gaaagcggcg acatcctcag tcatcacggt
     3601 tggctgcatc tgccggatca caaagcgggc ttcagcgaag agcagcaggc catctggcaa
     3661 aaagcagagc cgctgtttgg tgacgagccg tggtgggtgc gtgatctggc aaaagagacg
     3721 ggtaccgacg agcaggcaat gcgcctgacg ctacgccagg cggcgcagca aggaataatt
     3781 accgcgatcg tcaaagatcg ttattaccgt aacgatcgga ttgtcgaatt tgccaatatg
     3841 atccgcgatc tcgatcagga gtgtggttca acctgcgcgg cggatttccg cgatcgctta
     3901 ggcgtaggcc gaaagctggc aattcagatt ctggaatatt ttgaccgcat tggctttacg
     3961 cgtcgtcgtg gaaatgatca tttattgcgc gacgcattat tatttccgga aaaataagga
     4021 aatgattaat taatttttaa aat
//
LOCUS       DAZSBF010000303         3959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZSBF010000303 DAZSBF010000000
VERSION     DAZSBF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.308"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..1898
                     /gene="tssM"
                     /locus_tag="ACHXWD_004024"
                     /pseudo
     CDS             1..1898
                     /gene="tssM"
                     /locus_tag="ACHXWD_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3358
                     /locus_tag="ACHXWD_004025"
                     /pseudo
     CDS             1909..3358
                     /locus_tag="ACHXWD_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            3285..3767
                     /locus_tag="ACHXWD_004026"
     CDS             3285..3767
                     /locus_tag="ACHXWD_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR7622765.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         1063 a         1020 c         1064 g          812 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggca
//
LOCUS       DAZSBF010000304         3959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZSBF010000304 DAZSBF010000000
VERSION     DAZSBF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.309"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHXWD_004027"
     CDS             complement(<1..242)
                     /locus_tag="ACHXWD_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7622766.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHXWD_004028"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHXWD_004028"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR7622767.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHXWD_004029"
     CDS             2255..3904
                     /locus_tag="ACHXWD_004029"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR7622768.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZSBF010000305         3950 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZSBF010000305 DAZSBF010000000
VERSION     DAZSBF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.310"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHXWD_004030"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHXWD_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR7622769.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHXWD_004031"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHXWD_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR7622770.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHXWD_004032"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHXWD_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR7622771.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHXWD_004033"
     CDS             complement(3668..3898)
                     /locus_tag="ACHXWD_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR7622772.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1008 c         1001 g          918 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ccaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag
//
LOCUS       DAZSBF010000306         3940 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZSBF010000306 DAZSBF010000000
VERSION     DAZSBF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.311"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHXWD_004034"
                     /pseudo
     CDS             complement(1..2601)
                     /gene="acrF"
                     /locus_tag="ACHXWD_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHXWD_004035"
     CDS             complement(2613..3770)
                     /gene="acrE"
                     /locus_tag="ACHXWD_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR7622773.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT          961 a         1038 c          974 g          967 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
      841 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
      901 tcaggtaaga aggaagaagg aagacggaag acgtaaaaac aacaccacca ttcctgcaac
      961 aatcagcgca tagatcagta aatatcgtcc tgtggaaccg aggattttgc cgacgctgtt
     1021 ggtgtagtgg ttaacgctat gatcgaaggt ggtattaaac cagccgaaga aaccgccctt
     1081 attttcgtga tgctcagcag agacgggttt aagcagcgtt gcacataacg caggggtaag
     1141 aatcaatgcc accagaacag aaagcgccat tgccgaaacg atggtgatag agaactggcg
     1201 ataaattgcc ccagtagaac cgccgaagaa tgccatcgga ataaataccg ctgacagcac
     1261 catcgcgata cccaccagtg cgccctgaat ttgcgacatc gatttttccg tcgcttcttt
     1321 tggcgggagc ttatcctcca tcatcacgcg ctcgacgttc tccaccacca ctatcgcatc
     1381 atcgacgagc agcccgatgg caagcaccat cccgaacatc gttagtgtgt tgatggagta
     1441 accaaaagcg gcgaggatgg caaacgtccc taacaacacc acgggtaccg caatggtggg
     1501 gatcagcgtt gctcgcatat tctgcaagaa cagatacatc accaggaaca ccagcataat
     1561 ggcttcgaac agcgttttta ccacttcgtg aatagaaagc tggacgaatg gcgtggtgtc
     1621 ataagggtag agaaccttca ttccctgcgg gaagaatggc tgtaattccg ccagttttgc
     1681 cttaatggct ttcgcggtat tgagagcgtt cgcgccggtt gctagcttaa tccccaggcc
     1741 cgccgccggt tttccgttga tacgagcgat aacgttatag ttttcaccgc caagttcaac
     1801 tcgtgcgaca tctttcaggc gtaccaccga gccgtcactg tttacgcgca gggttacttt
     1861 gccgaattct tccggatttt ttaaccgcgt ctgagcaata atcgaggcgt tcaactgttg
     1921 ccctggtaac gctggcgttc cgcccaactg cccggcagcg atctgatcgt tctgtacctt
     1981 caactggtta atcacatcaa ccggtgtcag tttatatttg tttagcagat cggcgtccag
     2041 ccagatacgc atcgcatact gtgcgccgaa aagctgtacg tcaccgacgc cattcagacg
     2101 gctaagcgta tctttaacgt tagaggccac atagtccgag atatcgtcct gtgtggtgtc
     2161 tgggttatca gagacaaagc ccgccaccat caaatagctg ctactggact tttcaacact
     2221 gatcccctgc tgctgaacct cctgcggcag caacggcgtg gcgagctgga gtttgttctg
     2281 cacctgcact tgcgcgatat caggatcggt cccggactgg aaggtaaggg taattgtcac
     2341 gctaccggcg gaatcgctgg tggaggacat atacatcagg ttatcgatac cattcatatt
     2401 ctgttcgata acctgcgtca ccgtatcctg cacggtctgc gcatcagcac ctggatagtt
     2461 tgcctaaaca gaaaccgcag gcggtgcaat ggttggatac tgagcgacgg gcaattgtag
     2521 gatcgccagt gcgcccgcca tcatcagaat aatggccagc acccatgcaa atatcggtcg
     2581 tcgaataaaa aagtttgcca tgtcagatta ccttacttcg atgcagtatc tgcgggggta
     2641 tcggtagtgg ctttaacctg ctctcccgga cgcgcttttt gcaggccgct gacaatgact
     2701 tgatcgccag atttcagacc ttcgctaatc aaccatttat cgccaatcgc ctgactggca
     2761 acgacagggc gcgcttccac ctgactttta tcgttaacaa tcagcacggt tgcatcacca
     2821 cgcggtgtgc ggctaacgcc ttgttgcggg ataagaatgg cgtcaggttg gacgccttca
     2881 tcaatccgtg cacgcacaaa catacccggc aaaagcgtat gttgcgggtt agggaagaca
     2941 gcacgtaggg ttatggagcc ggtgcattca tcaacggtca catcagagaa ttgcagcgta
     3001 cctttcaggg gataggtttg accgttttcc atgaccaact ctacgttgct ggtggcgttt
     3061 tccttatgca aatttccttg ctctacggat tgcttcagcc tcataaaatc gttgctggat
     3121 tgggtcacat caacgtagat aggatcgagc tgctggacag tcgccagttc agtcgtttgc
     3181 ccattagtga caagagcgcc ttcggtcacg gtcgatttgc cgatacgtcc gctaattggc
     3241 gcagtcactt tggtataagc gagattgatg cgagcgcttt cgactgtggc ttttgcggca
     3301 atcacggcgg catcggcctg gcgagcatca gcaatggcct ggtcgtactc ctgctggctg
     3361 atgtatttcg tacccacgag cggaacgtaa cgttttaccg tcaaatgcgc gatggcggcg
     3421 gcggcttcac ttttcgccag ttcgcctttc gcgctgtcat aatttgcctg ataggtcgcg
     3481 ggatcgatct ggtacaggga ctggcctgct tgtacatcgc tgccttcagt gaaattacga
     3541 ttcagtacga tcccgctaac ctgtgggcga acttcggcta tacggtaagc attggtgcgg
     3601 cctggtaatt cagtcttaac ttctaacggg gccgttttca caatatgaac ggtaacctgc
     3661 ggttcaccga cgtgagcttt ctcttctccc ttatcgttac aaccgacgat taaagccgcg
     3721 gagatcagaa taaaggaggg caggaggaaa aacctggcat gtttcgtcat tactattcct
     3781 caaaaaaaca aaagcgcgtt atttacccag aaaggcaacg cgttaactcg cagaaagaaa
     3841 aatacagttt gctatcctac aaattatcat tcgtcgatgt aaggaatagt tatgaatact
     3901 ggcgtctcaa ggcacataaa cacaaaaaaa gattaatatt
//
LOCUS       DAZSBF010000307         3908 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZSBF010000307 DAZSBF010000000
VERSION     DAZSBF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.312"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHXWD_004036"
     CDS             24..590
                     /locus_tag="ACHXWD_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR7622774.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHXWD_004037"
     CDS             594..1169
                     /locus_tag="ACHXWD_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR7622775.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHXWD_004038"
     CDS             complement(1211..2263)
                     /locus_tag="ACHXWD_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR7622776.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHXWD_004039"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHXWD_004039"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR7622777.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHXWD_004040"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHXWD_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          951 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZSBF010000308         3864 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZSBF010000308 DAZSBF010000000
VERSION     DAZSBF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.313"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            750..1466
                     /gene="nanC"
                     /locus_tag="ACHXWD_004041"
     CDS             750..1466
                     /gene="nanC"
                     /locus_tag="ACHXWD_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR7622778.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1486..2592
                     /gene="nanM"
                     /locus_tag="ACHXWD_004042"
     CDS             1486..2592
                     /gene="nanM"
                     /locus_tag="ACHXWD_004042"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR7622779.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2657..3637
                     /locus_tag="ACHXWD_004043"
     CDS             2657..3637
                     /locus_tag="ACHXWD_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR7622780.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3747..3864
                     /locus_tag="ACHXWD_004044"
                     /pseudo
     CDS             3747..3864
                     /locus_tag="ACHXWD_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1162 a          801 c          876 g         1025 t
ORIGIN      
        1 cttcagaagc gaccagttac aagccacaaa tcaggaaaca tatttcatgg tgagaattat
       61 gatcagcctg atggcgggta aacacttgtt ttcaccacgc tcactgagtt agcttgcaaa
      121 gcttcccttg tttagaccct aaataaattt tttataaaac aaacagttaa aagaaatcct
      181 ttagtttttg aagaccacct caagtgttcg tctggcttca catggcatct tcctcttaga
      241 aaaagatcga catattttgt gacacgaatt gcaaatctcg ttttgttgta tggcttgcgt
      301 gatttttgat ctggtataac aggtataaag gtgcaccgcg atagtcaatg agacagggca
      361 tctcgcaatc tatggcaaac atcacttcag ttctttctca tcgggtgatg aaaacgcact
      421 tcagtctgaa aggaatatga aaatgagatc aacagacact ctattttatg actctgggta
      481 aaatggattg agtaagtgat atagcttacg aacattcaaa tcaattaaac atcagaagag
      541 attttatact caggtattta atctgattct ctgtttattt aaataatgtg gaaagagaat
      601 tttcacagga gaccttatac aaaaatataa aataagctac ccgttgccaa agacactata
      661 agcctggcaa aaaatattac acaacataaa tgctaattgt ttatgcgggc tttgtattgc
      721 tttctgtatc ctacaaatga gtgaaattta tgaaaaaggc taaaatactt tctggcgtat
      781 tattactgtg cttttcgtca ccattaattt ctcaggctgc gacactggac gtacgtggtg
      841 gatatcgtag cggaagccat gcctatgaga ctcgactcaa agtcagtgag ggatggcaaa
      901 atggatggtg ggcaagcatg gaaagtaata cctggaatac cattcatgat aataaaaagg
      961 aaaatgccgc actcaatgat gttcaggttg aagttaatta cgcgattaaa cttgatgatc
     1021 aatggacggt gcgcccggga atgttaacgc attttagcag caacggcaca cgctacggac
     1081 cctacgtaaa actgtcctgg gacgcgacaa aagatcttaa ttttggcatt cgctatcgtt
     1141 acgactggaa agcttaccga caacaagact tatccggtga tatgtctcgt gataacgttc
     1201 atcgttggga tggatatgtc acttaccata ttaatagtga tttcaccttc gcatggcaaa
     1261 cgacgctata cagcaaacag aatgattatc gctatgcaaa ccacaagaaa tgggcgacgg
     1321 aaaatgcctt tgttctacaa taccatatga cgcccgatat tacgccatac atagaatatg
     1381 actaccttga ccgccagggt gtttacaacg gcagagataa tttatcggaa aacagttatc
     1441 gcattggtgt gtcatttaaa ctgtagtaga caggagacag tcacaatgaa taaaactata
     1501 atggcgcttg ctatcatgat ggcttcattt gccgcaaacg cgtctgtatt accggaaact
     1561 cctgtgccat ttaaaagtgg taccggagca attgataacg acactgtcta cattggttta
     1621 ggtagcgcag gtacagcatg gtacaagcta gatacacagg ccaaagataa aaaatggact
     1681 gcgttagctg cattccctgg tggaccgaga gatcaagcaa cctctgcatt tattgatggc
     1741 aatctatatg tgtttggcgg cattggcaaa aacagcgagg gattgactca ggtatttaat
     1801 gacgtacaca aatacaaccc caaaaccaat agctgggtta aattgatgtc gctcgcgccg
     1861 atgggcatgg cgggtcatgt gacttttgta cacaacggca aggcttatgt tactggcggt
     1921 gttaaccaga atatcttcaa tggctatttt gaagatctca acgaggctgg aaaagattca
     1981 accgctatag ataaaatcaa cgcccactat tttgacaaaa aagcagaaga ttatttcttc
     2041 aataagtttc tgttgtcttt tgatccctca acacagcaat ggagttacgc tggcgaatcg
     2101 ccctggtacg gaacggctgg tgcggcggtt gtgaataaag gtgataaaac ctggcttatt
     2161 aatggcgaag ccaaaccagg attgcgaacg gatgccgtat ttgaacttga tttcaccggt
     2221 aataatttaa aatggaataa gcttgatccc gtctcatcac cagatggcgt cgctggcggt
     2281 tttgcgggga taagcaatga ttctcttata tttgccggag gggccggatt caaaggttca
     2341 cgagaaaatt accagaacgg taagaactat gcgcatgaag gcctgaaaaa atcatatagc
     2401 actgatattc atctttggca taacgggaaa tgggataaat cgggtgaatt atcgcaaggt
     2461 cgggcctacg gagtatcatt gccctggaat aatagtttat taattattgg cggtgaaact
     2521 gcaggcggca aagcggtgac ggattcagtt ttaatttctg tgaaggataa taaagtcaca
     2581 gtacaaaact aacgcttcag ggccccggta aaggggccct gctatcaatt tgtcatttgt
     2641 attacgcgaa aattatatga acgcaataat atcgcccgat tattactatg ttcttaccgt
     2701 tgctggtcag tctaatgcca tggcgtatgg cgaaggactg ccattaccgg acagggaaga
     2761 tgcgcctcat tccagaatta aacaattagc gagatttgcg catacgcatc ccggaggccc
     2821 gccatgtcac tttaacgaca ttattccact gactcactgc ccacacgatg ttcaggatat
     2881 gcagggttat caccatcctc tggcaacgaa tcatcaaaca cagtacggca ccgttggcca
     2941 ggcactgcat attgcacgga aattactgcc cttcattcct aataatgcag ggattcttat
     3001 cgttccgtgt tgccgtggcg gatcggcttt taccgcgggc agcgaaggga catattcaga
     3061 acggcacgga gccagccatg atgcttgtcg ttggggaacg gatactccgc tgtaccagga
     3121 tttagtcagc agaacgcgag ccgcactggc aaaaaatccg cagaacaaat tcctcggcgt
     3181 atgctggatg caaggcgaat ttgacttaat gaccagtgac tacgcgtcac accctcaaca
     3241 ctttaatcat atggttgaag cctttcgtag ggatctaaaa caataccatt ctcagcttaa
     3301 taatattact gacgcgccat ggttttgcgg cgataccacc tggtactgga aagaaaattt
     3361 cccgcattcg tatgaagcta tttatggcaa ttatcaaaat aatgttttag ccaatattat
     3421 tttcgtcgac ttccagcaac aaggtgaaag aggactgacg aacgcgcctg atgaagaccc
     3481 ggacgattta agcacgggat attacggttc agcgtaccgg tcaccggaga actggacgac
     3541 ggcactgcga agcagtcatt tcagcacggc agcccgtcgg gggattattt ctgacagatt
     3601 tgtagaagca attttgcagt tttggcgcga aaggtgagcg gggagtttat tcatggggga
     3661 tgtccttgtt cgttctcaga gtagacaaca aaaaacaaat aagatcaagg taatatctct
     3721 tcacttgcgg gctcgaacag caggcaattg ccaatataat aatctggcac aagggtgaaa
     3781 aacgcgcacc gcataaaccg ttgttattgc ttacagatta gccggatgcc ttaatagaca
     3841 tccgcgcctt ttcgattatg gctc
//
LOCUS       DAZSBF010000309         3864 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZSBF010000309 DAZSBF010000000
VERSION     DAZSBF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.314"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHXWD_004045"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHXWD_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="ACHXWD_004046"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="ACHXWD_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR7622781.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHXWD_004047"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHXWD_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR7622782.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHXWD_004048"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="ACHXWD_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR7622783.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          845 c          959 g         1072 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc ttgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAZSBF010000310         3858 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZSBF010000310 DAZSBF010000000
VERSION     DAZSBF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.315"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHXWD_004049"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHXWD_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR7622784.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHXWD_004050"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHXWD_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR7622785.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHXWD_004051"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHXWD_004051"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR7622786.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHXWD_004052"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHXWD_004052"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR7622787.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          911 a         1049 c          942 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagac
//
LOCUS       DAZSBF010000311         3834 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZSBF010000311 DAZSBF010000000
VERSION     DAZSBF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.316"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..282)
                     /gene="chbB"
                     /locus_tag="ACHXWD_004053"
     CDS             complement(<1..282)
                     /gene="chbB"
                     /locus_tag="ACHXWD_004053"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR7622788.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLK"
     gene            complement(581..919)
                     /gene="osmE"
                     /locus_tag="ACHXWD_004054"
     CDS             complement(581..919)
                     /gene="osmE"
                     /locus_tag="ACHXWD_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR7622789.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1121..1948
                     /gene="nadE"
                     /locus_tag="ACHXWD_004055"
     CDS             1121..1948
                     /gene="nadE"
                     /locus_tag="ACHXWD_004055"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR7622790.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2178..3065
                     /gene="cho"
                     /locus_tag="ACHXWD_004056"
     CDS             2178..3065
                     /gene="cho"
                     /locus_tag="ACHXWD_004056"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR7622791.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3025..3600)
                     /gene="ves"
                     /locus_tag="ACHXWD_004057"
     CDS             complement(3025..3600)
                     /gene="ves"
                     /locus_tag="ACHXWD_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR7622792.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT          979 a          950 c          916 g          989 t
ORIGIN      
        1 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg
       61 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
      121 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
      181 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga
      241 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
      301 gattatctgt cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt
      361 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
      421 agtatttctt tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca
      481 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat
      541 ggccggtttc cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat
      601 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
      661 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
      721 taccacgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
      781 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttag
      841 tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
      901 ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga
      961 atacattggg cgctttttca agcccgcgaa cgaaacggct ccgctttcag aggattcctg
     1021 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtgg caacgggtta
     1081 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     1141 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtat
     1201 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     1261 cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct
     1321 gcgcctggaa accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     1381 tcaggccgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     1441 aaccgttaat atcaagggcg cggtattggc cagcgaacag gcattgcggg aagcaggcat
     1501 tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     1561 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     1621 taccggattc ttcactaaat atggtgacgg cggtacagac attaatccgc tgtatcgtct
     1681 caacaaacgt cagggtaaac agttactggc ggcattaggt tgcccggaac acctttataa
     1741 gaaagcgcca acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact
     1801 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt
     1861 cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac
     1921 cgttttcgat gatttctgga aaaagtaata atttgcacat aatggtttgt gcgaaaaaga
     1981 gtaatttgtt cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa
     2041 ttcaatatat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc
     2101 tttgtcatcg acccgcctct tttttaatcg ctccccgcct gttacactgg atagataacc
     2161 agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
     2221 cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac
     2281 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg
     2341 ttaatatccg cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac
     2401 ggcaatcccg gcgaattagc tggatctgta ccgcaggcga aatcggcgct ctgctccttg
     2461 aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc
     2521 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
     2581 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
     2641 cattgcagac catcgctgat gaacaaaaac tttgttatgg cctgctggga ctggaaccgt
     2701 taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
     2761 gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg
     2821 tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc
     2881 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
     2941 caacgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
     3001 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
     3061 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
     3121 atcgaaccag cacgcgccct ggtcggtagt gagcaattta tcgcccagtt gccacgcccc
     3181 gttaatcaca aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat
     3241 tctgactttc gccttacaga catccaggcg tgtcatgatg ttgaaatcca tcgacatctg
     3301 cccttccgtc agtttcgctt ttaccacctg gtccgctgca aaggcaaaag gctgtaacgg
     3361 ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa
     3421 cgtcactatc ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc
     3481 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
     3541 ttcaccggca gcatttcgcc acagattcac cgacatttta cgcatatcaa agtattccat
     3601 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
     3661 caactcgggt gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga
     3721 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
     3781 cttaagtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttg
//
LOCUS       DAZSBF010000312         3831 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZSBF010000312 DAZSBF010000000
VERSION     DAZSBF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.317"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..265)
                     /locus_tag="ACHXWD_004058"
     CDS             complement(<1..265)
                     /locus_tag="ACHXWD_004058"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR7622793.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            352..759
                     /gene="nudG"
                     /locus_tag="ACHXWD_004059"
     CDS             352..759
                     /gene="nudG"
                     /locus_tag="ACHXWD_004059"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR7622794.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(725..997)
                     /locus_tag="ACHXWD_004060"
     CDS             complement(725..997)
                     /locus_tag="ACHXWD_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFR7622795.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHXWD_004061"
     CDS             1233..2576
                     /gene="gdhA"
                     /locus_tag="ACHXWD_004061"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR7622796.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2693..3732)
                     /locus_tag="ACHXWD_004062"
                     /pseudo
     CDS             complement(2693..3732)
                     /locus_tag="ACHXWD_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          932 c          917 g          985 t
ORIGIN      
        1 caagagaaat atcaagaaag ccgcccgcgt cggtaagctc tctgcgccgc gccagcgcac
       61 cgtccagacc atcaagcagc ctgttcaaca aaatgacgac tagcgccgcc agataccagc
      121 ccagcgccag aaacggcaga gccagcacgc cgatggcaaa tcccactaat gttaaaccat
      181 ctggcgtaat gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc
      241 ggggatgaag atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag
      301 caacagtgcg gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg
      361 attgaagttg ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc
      421 gcccagagcg atcaggcggg attatgggag tttgccgatg gtaaagtcga gctggatgaa
      481 agccagcagc aggcactggt gcgtgagtta aacgaagaat tgggcatcga agcaactgtg
      541 ggtgagtatg ttgccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc
      601 tggcacgtac ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg
      661 tgctcacctg aagaggcgct gcaatatccg ctggcccctg ctgacattcc actattagag
      721 gcgtttatgg ctttacgcgc cgccagagca gcggattagt gctaagggtt ttgtcatccc
      781 gctggcattg cagcagtatt ccttcggctt taattaccgc cccttcagaa taattttgat
      841 cctgataaac gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt
      901 ctggcggtac gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg
      961 gaaatgctaa tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt
     1021 ctctgcaact gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg
     1081 gttatgagat tactctcgtt attaatttgc tttcccgggt catttttttc ttgcttaccg
     1141 tcacattctt gatggtatag tcgaaaactg caaaagcaca tgacataaac aacataagca
     1201 caatcgtatt aatatataag ggttttatat ctatggatca gacatattct ctggagtcat
     1261 tcctcaacca tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg
     1321 aagtaatgac cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat
     1381 tactggagcg tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg
     1441 atcgcaacca gatacaggtc aaccgtgcat ggcgtgtgca gttcagctct gccatcggcc
     1501 cgtacaaagg cggtatgcgc ttccatccgt cagttaacct ttccattctc aaattcctcg
     1561 gctttgaaca aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg
     1621 gcagcgattt cgatccgaaa ggaaaaagcg aaggtgaagt gatgcgtttt tgccaggcgc
     1681 tgatgaccga gctttatcgt catctgggcg cggataccga cgttccggca ggtgatatcg
     1741 gcgttggtgg tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata
     1801 ccgcctgcgt cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag
     1861 ctaccggcta cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt
     1921 ttgaagggat gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa
     1981 aagcgatgga atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg
     2041 atgaaagcgg attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg
     2101 atggtcgagt ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc
     2161 cgtggtctgt accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg
     2221 acgccgcgca tcagcttatc gccaatggcg ttaaagccgt cgctgaaggg gcaaatatgc
     2281 cgaccaccat cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta
     2341 aagcggctaa tgctggcggt gtagcaacgt cgggcctgga aatggcacaa aacgctgcgc
     2401 gcctgggctg gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc
     2461 accatgcctg tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga
     2521 acattgccgg ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg
     2581 taaatgcctg atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta
     2641 cgctctaatg taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt
     2701 atttaacgga tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt
     2761 agtgctccca aaattgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact
     2821 ttctgcgata gcagcagata tgccagtaaa gaaaccccat tagactattt ttttgataat
     2881 cttcttcgct ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg
     2941 ccagttcttc ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag gtcatccatg
     3001 ataattccgc cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat
     3061 cagagatata gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg
     3121 gggcttcctc catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct
     3181 ccattaggca caataacttt cgctatcttc gataccccat tgattctcta aagactcgcg
     3241 tcttttactg atgatatcga tcgagtcaaa aggaagcaca tgatattgga aggtatcttt
     3301 accaggttca ggctttcgcg gccagaactc tagcgtttca gaccattgct tatgatagaa
     3361 tcgataaggt gcgatcaatt gtagcgcctg taacttctcg atactgagcg gctcaatacc
     3421 tttagcctga taataatgca gttgttcttt ttttgcttta aaaccggccc gaacaataag
     3481 ccccatcata attaatagat aaagaaaaga gcatcccgcg gtaatcaggc ctctttcatt
     3541 caaaccgttg gatgttatcg ctgcgaacac aaacattaca gcgacaacac atgttaaata
     3601 aaacccccac ttacaaagca gcatggcctt attttcttta atcatccgtt caaaattact
     3661 attaaatatt tcccagccat taaaagaata cttctcgctc ccaggatggt tttgtaataa
     3721 aacttttttc atcacattcc ctgttattac atactttaga ggcgataatt ttatccatgc
     3781 aaaaaaaata tccaacgaat tttcttgatc cgctggatat ttcagaatat g
//
LOCUS       DAZSBF010000313         3798 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZSBF010000313 DAZSBF010000000
VERSION     DAZSBF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.318"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            72..2351
                     /gene="ydeP"
                     /locus_tag="ACHXWD_004063"
     CDS             72..2351
                     /gene="ydeP"
                     /locus_tag="ACHXWD_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR7622797.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2599..2796
                     /gene="safA"
                     /locus_tag="ACHXWD_004064"
     CDS             2599..2796
                     /gene="safA"
                     /locus_tag="ACHXWD_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR7622798.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            2871..3632
                     /gene="ydeO"
                     /locus_tag="ACHXWD_004065"
     CDS             2871..3632
                     /gene="ydeO"
                     /locus_tag="ACHXWD_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR7622799.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT         1060 a          839 c          872 g         1027 t
ORIGIN      
        1 atccctttgt atgtactgtg catcatcgct attacaaatc ctaataattc atttccacac
       61 aggataagta gatgaagaaa aaaattgaat cctaccaggg tgctgcaggt ggttggggtg
      121 ctgttaaatc cgtagcgaat gcagtacgta agcagatgga tatacgccat gatgttattg
      181 ccatgtttga catgaataag ccagagggct ttgactgtcc gggttgtgca tggccagatc
      241 ctaagcacag tgcgtcattc gacatttgtg aaaacggcgc aaaagcaatc gcctgggaag
      301 tcacggataa gcaggtaaat gcctctttct ttgctgagaa tacggttcaa tcattactta
      361 cctggggaga tcacgagctt gaggctgcgg ggcgtctcac tcagcctttg aaatatgatg
      421 ccgtcagcga ctgttacaag ccattaagct ggcaacaagc tttcgacgaa attggcgcac
      481 gccttcaaag ctatagtgat cccaatcagg ttgaattcta tacttcgggc cgcacttcca
      541 atgaagctgc ctttctttat cagctttttg cccgtgaata cgggagcaat aactttcccg
      601 actgctccaa catgtgccat gaaccgacaa gcgtgggttt ggcagcgagt atcggtgtag
      661 gtaaagggac cgtgttgctg gaagactttg agaagtgcga tttagtcatt tgcattgggc
      721 ataaccctgg tacaaaccac cctcgcatgc tgacttcgtt gcgcgcttta gtgaaacggg
      781 gagcgaaaat gatcgccatc aatcctctac aggaacgtgg cctggagcga tttaccgcac
      841 cgcaaaaccc gtttgaaatg ctgacgaact ctgagactca gttggccagt gcctactaca
      901 acgtgcgcat tggtggcgat atggcgttgc tcaaggggat gatgcgcctg ttaattgagc
      961 gcgatgatgc tgcaagtgcc gcaggtcggc cctcattgct tgatgacgaa tttattcaaa
     1021 cgcataccgt cggctttgac gagctacgcc gtgacgttct caattccgag tggaaagata
     1081 tcgaacgtat ttctggacta agtcagacac aaatcgccga actggctgac gcatatgccg
     1141 ctgccgatcg caccattatc tgttacggaa tggggatcac tcagcacgaa catggtaccc
     1201 agaatgtaca gcaactggtc aatctgctgt tgatgaaagg taacattggc aagcctggtg
     1261 cgggtatctg cccactacga ggtcactcta atgtacaggg cgaccgaacc gtcggtatca
     1321 ccgagaaacc gtctgcagag tttctggctc gcctgggtga gcgctatggc ttcaccccac
     1381 ctcatgcacc tggacatgct gcaattgcca gcatgcaagc aatatgcacg gggcaggctc
     1441 gagcattgat ctgcatgggg ggcaactttg cactggcaat gccagatcgg gaagcgagcg
     1501 ctgtaccgtt aacgcaatta gatttggcgg tacacgtagc cactaagctt aaccgctctc
     1561 atctgctgac cgcacggcat agctatattc tgccggttct ggggcgtagc gagattgaca
     1621 tgcaaaaaag cggtgcgcag gcggtaaccg ttgaggattc aatgtcgatg attcatgcct
     1681 cgcgtggcgt gttaaaaccc gccggtgtaa tgctgaaatc agagtgtgca gtggtcgcgg
     1741 gaatcgcgca ggcagcacta ccccacagcg tggtagcctg ggagtatctg gtggaagatt
     1801 atgatcgcat tcgcaatgac attgaagctg tgttaccaga gttcgccgac tataaccagc
     1861 gcatccgaca tcctggaggt tttcacctga taaatgcagc tgctgaaagg cgctggatga
     1921 cgccgtcagg taaggctaat ttcattacca gcaaagggct gttagaagat ccctcttcag
     1981 cgtttaacag taagctggtc atggcgacag tacgtagcca cgatcagtac aacacgacga
     2041 tttatggtat ggatgatcgc tatcgagggg tattcggtca acgagatgtg gtctttatga
     2101 gtgctaaaca agctaaaatt tgccgtgtaa aaaacggcga aagagttaat cttattgcgc
     2161 ttacgccaga cggtaagcgc agctcacgcc gcatggatag attaaaagtg gtcatttacc
     2221 ctatggctga ccgctcactg gtgacctatt ttccagaatc gaatcacatg ctaactcttg
     2281 ataaccacga tccattaagt ggcattcctg gttataaaag tattccggtt gaattagaac
     2341 catcaaatta attcgcctcc tcatttcttc tgctgtcatc cgcacagcag aagaatttct
     2401 cattgactat tatcgcgcaa tttgctcaca ttgattaaat taaaccacac actataagat
     2461 ataaacctct gcctacagct gtaagaaact ccgctcagta ctgaagtgcc agtcctattt
     2521 cctcttttct ccagcctgtt atattaagca tactgattaa cgatttttaa cgttatccgc
     2581 taaataaaca tatttgaaat gtatgcgacc acagtgaaaa acaaaatcac gcaaagagac
     2641 aactataaag aaatcatgtc tgtaattgtg gttgtcttat tactgaccct tacgttgata
     2701 gccatttttt cggcaattga tcagctgggt atttcagaaa tgggtcgcat tgcaagagat
     2761 cttacacatt tcattatcaa tagtttgcaa gactgaaaat aaacagcaaa ttataaatat
     2821 gaaatgttaa aaaagtatcg ataaaaactt tattgtttta aggagataaa atgtcgctcg
     2881 tttgttctgt tatatttatt catcatgcct tcaacgctaa cattttagat aaagattacg
     2941 ccttctctga cggcgagatc ctgatggtag ataacgctgt tcgtacgcat tttgaacctt
     3001 atgagcggca ttttaaagag atcggattta ctgaaaatac cattaaaaaa tatctacaat
     3061 gcactaacat ccagacagtg acggtgcctg ttcctgcgaa gtttttacgt gcttcaaatg
     3121 taccgactgg attgcttaat gaaatgattg cttatcttaa ctcggaagaa cgcaatcatc
     3181 ataatttttc agaacttttg cttttttctt gcctgtctat ttttgccgca tgcaaaggtt
     3241 tcattacact attaactaac ggtgtgctat ccgtttccgg aaaagtgaga aatattgtca
     3301 acatgaagct ggcgcaccca tggaagctga aagatatttg tgactgcctg tacatcagtg
     3361 aaagcctgtt gaagaaaaaa ctaaagcaag agcaaacgac attctcacag attctattag
     3421 atgccagaat gcagcacgca aaaaatttga tacgcgtaga aggttcagtc aataaaattg
     3481 ccgaacaatg tggttatgcc agtacatctt attttattta tgcgttccgc aaacatttcg
     3541 gcaacagtcc gaagagagtt tctaaggagt accgttgtca aagtcacatg ggtatgaata
     3601 cgggcaacac gatgagtgct ttagctattt gattatttgc taacgagtag tcaaccacgc
     3661 acgctgcgta agaattaaat ggggcagcca ttccctgccc cgcgttgttt ttaggcgata
     3721 tatttattga aataaataag tgacatccat cacatattta tgcacttgca taacctgttg
     3781 cagattattt atgatctc
//
LOCUS       DAZSBF010000314         3797 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZSBF010000314 DAZSBF010000000
VERSION     DAZSBF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.319"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHXWD_004066"
     CDS             <1..81
                     /locus_tag="ACHXWD_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622800.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHXWD_004067"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHXWD_004067"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR7622801.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHXWD_004068"
     CDS             1027..1485
                     /locus_tag="ACHXWD_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR7622802.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHXWD_004069"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHXWD_004069"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR7622803.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHXWD_004070"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHXWD_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR7622804.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="ACHXWD_004071"
     CDS             2691..3368
                     /locus_tag="ACHXWD_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR7622805.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHXWD_004072"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHXWD_004072"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR7622806.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          952 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 ccacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAZSBF010000315         3775 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZSBF010000315 DAZSBF010000000
VERSION     DAZSBF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.320"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHXWD_004073"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHXWD_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR7622807.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHXWD_004074"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHXWD_004074"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR7622808.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHXWD_004075"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHXWD_004075"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR7622809.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          868 a          987 c          912 g         1008 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatcgcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgtga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgctcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg cggtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catag
//
LOCUS       DAZSBF010000316         3734 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZSBF010000316 DAZSBF010000000
VERSION     DAZSBF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.321"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHXWD_004076"
     CDS             complement(<1..181)
                     /locus_tag="ACHXWD_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR7622810.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHXWD_004077"
     CDS             425..682
                     /locus_tag="ACHXWD_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR7622811.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHXWD_004078"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHXWD_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR7622812.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHXWD_004079"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHXWD_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR7622813.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHXWD_004080"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHXWD_004080"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR7622814.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3734)
                     /locus_tag="ACHXWD_004081"
     CDS             complement(3307..>3734)
                     /locus_tag="ACHXWD_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR7622815.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          953 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aatt
//
LOCUS       DAZSBF010000317         3674 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZSBF010000317 DAZSBF010000000
VERSION     DAZSBF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.322"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..402)
                     /locus_tag="ACHXWD_004082"
     CDS             complement(<1..402)
                     /locus_tag="ACHXWD_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7622816.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKL"
     gene            454..1059
                     /gene="wrbA"
                     /locus_tag="ACHXWD_004083"
     CDS             454..1059
                     /gene="wrbA"
                     /locus_tag="ACHXWD_004083"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR7622817.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1080..1307
                     /locus_tag="ACHXWD_004084"
     CDS             1080..1307
                     /locus_tag="ACHXWD_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR7622818.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(1345..2586)
                     /gene="agp"
                     /locus_tag="ACHXWD_004085"
     CDS             complement(1345..2586)
                     /gene="agp"
                     /locus_tag="ACHXWD_004085"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR7622819.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2880..3674)
                     /locus_tag="ACHXWD_004086"
                     /pseudo
     CDS             complement(2880..3674)
                     /locus_tag="ACHXWD_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          972 a          886 c          819 g          997 t
ORIGIN      
        1 caacttcaca gggtctgtta aggctgactt gtcgtagagt tcaccatcac ccagagtcag
       61 tatagagacc cccttcctca gtatctgcct gacatcatct atagctgtgt cgatatcagc
      121 cctactgaag cggtcaaagt tctctacaac aatcaaggaa ccttctggaa tagccccaga
      181 cataacattt tcaaacatac gtctgaagtc agaaccttga ttcttatgtt gctggtgaaa
      241 accagattta cccttatcaa catatctgtg agcaggaaga aggtaataat cgtttttgtg
      301 cttagagtag aaagcatcaa tgaggtttgt ttgccgtctt aaggagtctc ctgagtcttg
      361 ctctttagta ctccacctga tgtatgtcac tagcagcttc atatcttcgg actatgctta
      421 tctaagtggt tcattgaaca ggagaacaat gatatgagtg ttaaggcaag aatactagtg
      481 ctttattatt ccatgtacgg acatattgaa acgatggcac gcgcagtcgc tgagggtgca
      541 agcaaagtcg atggcgcaga agttgtcgtt aagcgtgtac cggaaaccat gccgccgcaa
      601 ttatttgaaa aagcaggcgg taaaacgcaa actgcaccgg ttgcaacccc gcaagaactg
      661 gccgattacg acgccattat ttttggtaca cctacccgct ttggcaacat gtccggtcaa
      721 atgcgtacct tcctcgacca gacgggcggc ctgtgggctt ccggcgcact atacggaaaa
      781 ctggcgagcg tctttagttc caccggtact ggcggcggtc aggaacaaac tattacttca
      841 acctggacga cccttgcgca tcacggcatg gtgattgtcc ccattggcta cgcagcgcag
      901 gaattatttg acgtttcaca ggttcgcggc ggtacgccgt acggcgcaac caccatcgca
      961 ggcggtgacg gctcacgcca gccaagccag gaagaactgt ctattgctcg ttatcaaggg
     1021 gaatatgtcg caggtctggc agttaaactt aacggctaat cttcaacagg aggatacgca
     1081 tgccaactca agaagcgaaa gctcatcacg tcggtgaatg ggcatctctg cgcaatacgt
     1141 cgccggaaat agccgaggcc attttcgaag tcgccgggta cgacgaaaaa atggcagaaa
     1201 agatttggga agaaggtagc gatgaagtgt tagtcaaagc ctttgctaag gccgataaag
     1261 attcgctttt ttggggcgaa cagaccatcg aacgtaaaaa cgtttaacca gcgactcccc
     1321 cgcttctcgc gggggagttt tctgttattt caccgcttca ttcaacacgc tatcaaattt
     1381 atccatcggg cagaaaccgt tggcatctat cgggcaaccg cttaattcca gcgtcacacg
     1441 ctgagcaggt gcctgcaggg ttaacgcatc ggcattacgt aactgttccg cactctgata
     1501 cacatattca attttcatca aatcgcgatt ggctttgctg tcatgccaac gctggaaaac
     1561 gattttgccg ccaatcggcg tgcgttcgtt ctggtcatgc aactgatacg gtttgaaatc
     1621 cagcgccgtt aacagagagg caatgttgga gtcgtgccca accaacactg taattttcgg
     1681 tgcgctggtg cgatcggtga ccagagcttt gtcgatataa ctgaccagcg gtttcgcaac
     1741 attgcgcgcc acttccggtg aggtaaacag gctgtcctgg tagccgtttt taagcttcga
     1801 caacaccttc cactgctggt cagatttgat ttctccccag gccacctgat ccatcggaaa
     1861 accttcgtaa tattgcaaag taaacgcatc taccagcgag ttgccgactt tcagcggccc
     1921 ggaaacacct ggttcttgtt gatacttggc gctaaaggta tttttgccat ccaccagcga
     1981 acactgttgt ttctctttac aggcagggga atctttatag ttaacgattt tttccagtag
     2041 ctggtagctg tcggtaagct ggagtttgct gagctctttc tccattgccg ccaccgcctg
     2101 ttcactgaat gcggcggaat catcggtgat caccgggtta aaggttgggt ccatagtgcc
     2161 cattttttcc tggtgatgca caggaatatc acaccccggg aatgcgccgg taataaagaa
     2221 ctgtgcggtc gcaacggtac gttgcagact attggcatag gcataaacgg tgtccggcgg
     2281 cgggcattcc cccgatttca ccatcccctg ctctgccagc cattcacgca tgtaatggcc
     2341 catatacact tcgagcacgc cacctttggt ggtgagttgc ccaccgggga cgtcccattc
     2401 tggccattta ttcggcgtcg actgctccag cacactgcca ttgttcgcca gcggcgcacg
     2461 taagttatgg cggctcatca tgagcacttg ctgtagctga tagccttccg gtacggtttg
     2521 tgcctgagca tttgaagcga gtaaaactat ccctgccaca gttgcggcga ttagcgtttt
     2581 gttcattcct ggcacctctt ttgttatcaa tcattaaaga gtgtgacaga aatatgactt
     2641 ttttccggga acaggttcgc aaaatggctt gttgaacgcc tgtttttcta tatacatctt
     2701 caaaaacagg caaggtttga cgcaaactat gcctgattag cccctgcctc gcgtacgccg
     2761 ctgataattg cacctgtgga taaacagaat atcagcggcg ttactaatac agaggcaata
     2821 gtaccaataa taagagaagt gaggctaata acaatggttt ttagcacatg cctttgtcat
     2881 taaattgcat aacgagttaa aaaatcaatt tgcgcattcc tttccttatt gtttaactcg
     2941 taagcctgag caaaaagttc aggagggagg taaacgtcat aatgattgcc agtgtttatt
     3001 aaatacccat actcaaggtc gtcatctgtt gaaaaggatt cattccctaa ggagattgat
     3061 gccacattgc tgtaaacaat aaataatgag caaatgctat ctgaaagagc agtgaggaaa
     3121 tttttcacct cattaacctt ctgctctggg tcggtgtcta aaacaaggcg tgccttgtta
     3181 tcattaaata taaattcggg taaatgcagt atgcattttt tgtcattcaa gcaactatca
     3241 ataaattctt ttttaaaaga caaaaaactc catgataaaa aaccaccctg cccctgaatt
     3301 ttgttgtcag attgtagatt aacgccaaat aacgtcttta catcttccct gagattatag
     3361 aatgccgtat caacaagagc ctcgatcgtt tgtttatcaa caattttaat aaactcttcg
     3421 tatacttgct tttgcgtctt atggccgccc agcgcccatg aatactcatg ttcttttaaa
     3481 accagtatct aaatcagata ttcccggcgt atttatctgt tctggctgga tactcatatt
     3541 ttcatcatct aaacaatact cagaaaaaat gtcatcacac gcagtaatgt tttcaatata
     3601 ttccataaag ccataccaat aataaccttt aaaatctgtg atagactctg aacctctgcc
     3661 cggcaaggac actc
//
LOCUS       DAZSBF010000318         3628 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZSBF010000318 DAZSBF010000000
VERSION     DAZSBF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.323"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..76)
                     /locus_tag="ACHXWD_004087"
     tRNA            complement(1..76)
                     /locus_tag="ACHXWD_004087"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41..43),aa:Thr,seq:tgt)
     gene            complement(79..155)
                     /locus_tag="ACHXWD_004088"
     tRNA            complement(79..155)
                     /locus_tag="ACHXWD_004088"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(119..121),aa:Arg,seq:tct)
     gene            complement(157..233)
                     /locus_tag="ACHXWD_004089"
     tRNA            complement(157..233)
                     /locus_tag="ACHXWD_004089"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:Ile,seq:cat)
     gene            complement(423..986)
                     /locus_tag="ACHXWD_004090"
     CDS             complement(423..986)
                     /locus_tag="ACHXWD_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR7622820.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1090..1308)
                     /locus_tag="ACHXWD_004091"
     CDS             complement(1090..1308)
                     /locus_tag="ACHXWD_004091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622821.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1467..1853)
                     /locus_tag="ACHXWD_004092"
     CDS             complement(1467..1853)
                     /locus_tag="ACHXWD_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR7622822.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1843..2214)
                     /locus_tag="ACHXWD_004093"
     CDS             complement(1843..2214)
                     /locus_tag="ACHXWD_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR7622823.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(2214..2471)
                     /locus_tag="ACHXWD_004094"
     CDS             complement(2214..2471)
                     /locus_tag="ACHXWD_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622824.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2468..>3628)
                     /locus_tag="ACHXWD_004095"
     CDS             complement(2468..>3628)
                     /locus_tag="ACHXWD_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR7622825.1"
                     /translation="ITDTGRKTWVKSSLEHYVAALRRNAALRDAEKTLNEALQKLRDA
                     HTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEVVERVECRNQGLEKSRTLMT
                     GIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKVTEQGHGVLLFTMEMANIQI
                     GERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTGRNIWMVDQANLTIDEICAT
                     TKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISKGLKGLAKSGGFPLIALSQL
                     SRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVYNPDTQATGIAEINITKQRN
                     GSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYSNTRTDSSKMERFF"
BASE COUNT          846 a         1007 c          812 g          963 t
ORIGIN      
        1 tggtgccgac taccggaatc gaactggtga cctactgatt acaagtcagt tgctctgcct
       61 gctgagctaa gtcggcgctg gcccaccacc gaggactcga acctcgcacc gtcaacttag
      121 aaggttgatg ctctatccgg atgagctagt ggtggttggt ggcccttgct ggacttgaac
      181 cagcgacctg gcgattatga gtcgctcgct ctgaccaact gagctaaagg gccggaggca
      241 gaataataac catatgtcat cacatctgca aactcatctg accaccagca cgtttaacgt
      301 cctgtgccgt ttttcaggta taaaaaaacc cgctcatcgg cgggtttaag ctgtgtggcg
      361 tagtaaccac tcttaacagg atattcaact ttttacgatc gtaaagtttt cggaaaaaat
      421 ttttaaaacc gcatcaaccg ctccaccagt tgctctttac gggcaacgat ccacccgtgt
      481 tgctccagat aaaatttaaa ccgttccaga gtacatacca tcgcatcggc gggtaccttt
      541 tccgtgaact caacctgacc gtgtttatcg aagtggctca gtaatgcgca tccatcattt
      601 tcgatagatg tgttttgtgc cgctggtggc tgtttctggt tgaaataaca gtcttccagt
      661 ttttcgaaca cttcccacgc ctgatcggtt tcgagcattt tggcgtgacg ggctgcgcca
      721 cgttctgtcc agaggatgag ggagcgaacg ttacgggcaa ttttcacaga aactcttaaa
      781 gagttttcgt tcttcaactc tcgtaacgca tcaccttcta tcaggaaaaa atgtttacct
      841 tctataaacc gttctttgtt tcgattgaaa ttgtttgtca aacgctggcg ctctgttccg
      901 tacaaatcag ccagcagctc tgttgtcatg acaggaagat ggttatgcgt aagtgacggg
      961 agtttttcaa tagacgtgcg attcatagac atgtcctttc gaatattttg atttacccct
     1021 tttgagaggg tgaccgggcg ctcaaaaccg tcgaaagtcg gcgggcgtat ttccattgct
     1081 ggtattgtat tagccgcaca cccggtcata aaccaagaat tctggacaca aaaaaaccac
     1141 atggctatcg ggtgtggata ccgctttcga ggtgttttga gcaccgcggg gaaatgtaaa
     1201 ctgcaaacct gaaactgtca acaccgtcag gttccaatca tcgggtggtg agacgcacag
     1261 ggttggaact accgggaaac cgaccggcga gcttttcagc tcccccatgc gccccaccat
     1321 aattcagatg tgcgcgtgca tacgacaata aaaaacacgc tcgcggcgtg tgtctgtcgc
     1381 ggtctctatc cggggttcca atcccgacgc cagattttgc tggcgcgtga ggaatatagc
     1441 cccggacaat gtgtctggtc aagcacctac ataattcgtt ctacgtatct gtccatctcc
     1501 agccggatat caagcatcat caacatgcca tcaataaccc cttccgcttt ctgcaggcgc
     1561 ttgccaatac aggtatccga acacccatgt ttccgtgcca gactcataaa agtcattcca
     1621 cctacgtaat aatccaccag caaatcgtgc aaatcctgat ttttcttgtt caaccgggcc
     1681 atacagccac aaattatcat tgcatcatca tcagaacact gagggcgtga tttcactttc
     1741 gacggaatta accctttaaa accagcagcg atcgacgccc aggatacatc ttcatgagtg
     1801 tttgcagccc aagcccccca ccgttccata acctgctgaa tatcacgcgc catcgttatc
     1861 acctgtgatt tcataaatct tcacgcccag tcgcccacca ggaacgagct gaccgcgcac
     1921 aatattgatt tcatcaaact gctcgtcgtc tatgagtagt ccggcatgcg tcagcgcatc
     1981 cagtggtgct ttcaggatat tgtccaggtc acgacggcgc ttatccggtg gctctgcaat
     2041 aatctttatc gccagccttc cggacaggtt taatttcagc cgctgctggc gaacaattag
     2101 cgtcacatca cggcgataac gctcaccaac ttttgataca aaatatgtgc tgccacgacg
     2161 tcgccagtaa gtattcaccg tcggcggata aggtaaaata aactcatggc gcatcagcgc
     2221 agcacctcct gcaccatttt ttcaaacttt ccaactttgg tttccagctc tgccacacaa
     2281 tcaacgagct catccaccgc tttttgtgcg cgatgtttcg cctgcatcag ttccctgagc
     2341 gctggcacca tatcccgacg aatggcatct tttgttacac ctgttttttc cagttgttcc
     2401 gcctgtcgca acatttcctg cgcgtgttta cgcaattgtt cagaggtaaa agtcattgtc
     2461 tggttgttca aaagaaacgc tccatcttac tgctgtcggt acgtgtattg ctgtatctgc
     2521 gcggctgggg ctgccgcatt ggggtggaaa gaacctgtgc gctttcctgg tccacaggca
     2581 gaaaatgtcc gttataaaaa cgccggtaaa tcgtccccag agaaccgtta cgttgtttcg
     2641 tgatattaat ttctgctatg cctgtagcct gtgtatccgg gttgtacact tcatccctgt
     2701 aaagcatcag aatgatgtct gcatccgcct ctatttctcc ggaatttttc aggtctgagt
     2761 tcatgggacg tttattgggt ctggactcca caccgcggga gagctggctc agcgcaatca
     2821 acggaaaacc accggatttt gccaggcctt taagcccctt tgagatttca cccacggcaa
     2881 ggtcatgacg ccccgtggtt cgggttttta tcagcccgag ataatcaacc accaccagcg
     2941 ccgtttccgg atgtttaatc agatggtgtt tcgttgttgc gcatatctca tcaatggtca
     3001 ggttcgcctg gtccaccatc cagatattgc gcccggtcat ccgccccacc ccttgtgaga
     3061 aacgcgccca gtcttcatct tcaaagtgag ccacagattt caggcgtgat actggcatcc
     3121 ctccagccgc agacaccata cgttcaccaa tctggatgtt cgccatctcc atggtgaaca
     3181 gaagcacacc atgcccctgc tcagtcacct tgtcgatgat gtccagcgca agttcggttt
     3241 tccccatcga aggacgggcg gcaatgaata ccaggtctcc gggctccata ccgcctgttt
     3301 ttgcgtccag ttcatcaata ccggtcatca acgtcctgga tttctccagc ccctgattcc
     3361 ggcattcaac acgctcaacc acttccggaa gcacatcatc aatatgtacc ggctgaatga
     3421 cgccctttcc tgtcgacagt gtgaccatca tgttctgcgc atccttcagg gcatcttcag
     3481 ctgcttcaca ggtatgcgca tcacgtaatt tctgcagcgc ctcattcagt gttttttctg
     3541 catcgcgcag tgcggcattg cgccgcaacg ctgcaacata gtgctccagt gaagacttca
     3601 cccaggtttt acgcccggta tcagtaat
//
LOCUS       DAZSBF010000319         3622 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZSBF010000319 DAZSBF010000000
VERSION     DAZSBF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.324"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            129..1232
                     /gene="proB"
                     /locus_tag="ACHXWD_004096"
     CDS             129..1232
                     /gene="proB"
                     /locus_tag="ACHXWD_004096"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR7622826.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGNSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1244..2497
                     /gene="proA"
                     /locus_tag="ACHXWD_004097"
     CDS             1244..2497
                     /gene="proA"
                     /locus_tag="ACHXWD_004097"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR7622827.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2612..2687
                     /locus_tag="ACHXWD_004098"
     tRNA            2612..2687
                     /locus_tag="ACHXWD_004098"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2645..2647,aa:Thr,seq:cgt)
     gene            3293..3451
                     /locus_tag="ACHXWD_004099"
     CDS             3293..3451
                     /locus_tag="ACHXWD_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622828.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          910 a          868 c          976 g          868 t
ORIGIN      
        1 gccgctaggc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca gatggttggc
       61 aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa attgaatggc
      121 agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt gtgctaacag
      181 gcggatcgcg ccgtctgaac cgtgcccata ttgttgaact tgttcgccag tgcgcgcagt
      241 tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc gccggacgtg
      301 agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg ctggcagcgg
      361 tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat ggcattcacg
      421 tcgggcaaat gctgctgacc cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc
      481 gcgacaccct gcgtgcgttg ctcgataaca atatcgttcc ggtaatcaat gagaacgatg
      541 ctgtcgctac ggcagagatt aaagtcggcg ataacgataa cctttctgcg ctggcggcga
      601 ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg tataccgctg
      661 acccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt gatgacgcac
      721 tgcgcgcgat tgccggtaac agcgtttcag gcctcggaac tggcggcatg agtaccaaat
      781 tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac cattattgcc gcgggcagca
      841 agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg ttccatgccc
      901 aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa
      961 tcacggtaga tgaaggggca actgccgcca ttctggaacg cggcagctcc ctgttgccga
     1021 aaggcattaa aagcgtgact ggcaatttct cgcgtggtga agtcatccgc atttgcaacc
     1081 tcgaaggccg cgatatcgcc cacggcgtca gtcgttacaa cagcgatgca ttacgccgta
     1141 ttgccggaca ccactcgcaa gaaattgatg caatactggg atatgaatac ggcccggttg
     1201 ccgttcaccg tgatgacatg attacccgtt aaggagctgg ccgatgctgg aacaaatggg
     1261 cattgctgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg
     1321 cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcatcc tcaacgctaa
     1381 cgcccaggat gttgctgacg cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct
     1441 ggcactgacg cccgcacggc tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct
     1501 cgccgatccg gtggggcagg taatcgatgg cggcgtactg gacagcggcc tgcgtcttga
     1561 gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac
     1621 ggttgatgtc gcttcgctgt gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa
     1681 agaaacgtgt cgcactaacg ctgcaacggt ggcggtgatt caggacgccc tgaaatcctg
     1741 cggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga
     1801 aatgctgcgt atggataaat acatcgacat gctgatcccg cgtggtggcg ctggtttgca
     1861 taaactgtgc cgtgaacagt cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca
     1921 tatttacgtt gatgaaagtg tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa
     1981 aactcagcgt ccgagcacat gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc
     2041 cgatagcttc ctgcccgcat taagcaaaca aatggcggaa agcggcgtga cattacacgc
     2101 agatgcagct gcactggcgc agttgcaggc aggccctgcg aaggtggttg ctgttaaagc
     2161 cgaagagtat gacgatgagt ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct
     2221 tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa cactccgatg cgatcctgac
     2281 ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg ctgtttacgt
     2341 taacgcctct acgcgtttta ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt
     2401 aagcacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga ccacttacaa
     2461 gtggatcggc attggtgatt acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt
     2521 agccatttga ttcataaggc cattgacgca tcgcccggtt agttttaacc ttgtccaccg
     2581 tgattcacgt tcgtgaacat gtcctttcag ggccgatata gctcagttgg tagagcagcg
     2641 cattcgtaat gcgaaggtcg taggttcgac tcctattatc ggcaccatta agaaaattaa
     2701 taacttagct taacttctct aagaaattgt atcttctgtt atcacttaca aacgcctata
     2761 cgttgttatg ggctttggta gtgtaacacg tatgggcgag tagttcccag gctcaacctc
     2821 gtaacaagtt cctttgaagc tggaagacgt gccaccgtct ctaaaccttc taccaaaact
     2881 cttagcaaga tgttccacac ctggcgcggt gtccaatgat gctacacgtc gtgagacgtt
     2941 gcagaggata aacaggctaa atgatgggaa gagttttaag tgcaatagtg gattaaagcc
     3001 cacctcttca aaacctaact tgatcgggaa agaatcaagc tctagcacgg tttcgtccac
     3061 cggagtacaa cgggtgcctg ataagagaag aactgctaag acttcatagc ttttgtgaaa
     3121 aagccctttt tagtggatac gcctacggcg cttatttttt ggtataacca tcaaaggcgc
     3181 tttatggctc ctattgtctt gaaaatgtat ctttacttgt attgtgtttt atgcgtagtc
     3241 tccctgtgta gtatgtaact tgttaatttt catatgaata cagaggcaca cgatgaaaaa
     3301 cattgcagca atcaaacgta ataaccgcaa gattcacgct cgtaagttcc tgtctacgcc
     3361 agaaggaaaa gcctggctag agcgtaaaca gagagagaac gaagaaagaa aactccttag
     3421 tgagttgaaa tggcttagag aggatttctg aaaatctcca gaacgcaaca ggacgcattc
     3481 taacgctgtt ttcattactc ggtataggga taggtgatgt gtgtttaatg cgtctgtgcg
     3541 cttgttttgt gccttatttt gcattttcat aatgcggaag agaacacgga aaaccttaaa
     3601 attaattatt ggggtattta ag
//
LOCUS       DAZSBF010000320         3603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZSBF010000320 DAZSBF010000000
VERSION     DAZSBF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.325"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            430..1419
                     /gene="araF"
                     /locus_tag="ACHXWD_004100"
     CDS             430..1419
                     /gene="araF"
                     /locus_tag="ACHXWD_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR7622829.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1489..3003
                     /gene="araG"
                     /locus_tag="ACHXWD_004101"
     CDS             1489..3003
                     /gene="araG"
                     /locus_tag="ACHXWD_004101"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR7622830.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3018..>3603
                     /gene="araH"
                     /locus_tag="ACHXWD_004102"
     CDS             3018..>3603
                     /gene="araH"
                     /locus_tag="ACHXWD_004102"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR7622831.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTT"
BASE COUNT          888 a          834 c          971 g          910 t
ORIGIN      
        1 agtgataatt aatggcgaaa tgaatgacgt gcattttcca catctttgag ttgcggttat
       61 tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt aaaaagaaga
      121 catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg gattaatctg
      181 ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt cccgctaaat
      241 ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg agaattaatt
      301 tctcgctaaa actatgtcaa cacggtcact tatcttttag ataaaaggta atgctttgtt
      361 ttccgattaa tttaacgaaa gtcattcgtt tttgccctac acaaaacgac actaaagctg
      421 gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc agccgttatg
      481 tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca accggaagag
      541 ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt agggtttgag
      601 gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga cagcctggct
      661 gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg ctctgccatc
      721 gtcgcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca gtttgttaac
      781 gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac taaaattggc
      841 gaacgtcagg gtcaggaact gtataaagag atgcagaaac gtggctggga tgtcaaagaa
      901 agcgcggtga tggcgattac cgccaacgaa ctggatacgg cacgtcgccg tacaacgggt
      961 tctatggatg cgctgaaagc ggccggattc ccggaaaaac aaatttatca ggtacctacc
     1021 aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt tcaacatccg
     1081 gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg cggcgtacgc
     1141 gcgacggaag gtcagggctt taaagctgcc gatatcatcg gcattggcat taacggtgtg
     1201 gatgcgttga gcgaactgtc taaagcacag gcaaccggct tctacggttc cctgctgcca
     1261 agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt agcaaaagac
     1321 gttgaaccgc caaaatttac cgaagttacc gacgtggtgc tgatcacgcg tgacaacttt
     1381 aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa aattcccctc
     1441 tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat gcaacagtct
     1501 accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa ggcgctgacg
     1561 gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga aaatggcgca
     1621 ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac gggttctgta
     1681 gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa cgcgggtgtg
     1741 gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga aaacatctat
     1801 ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa ttatgaggcg
     1861 ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct gaaatatctc
     1921 tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa cgctaaaatt
     1981 atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa tcttttccgc
     2041 gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca ccgtatggaa
     2101 gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta tgtcaaaacc
     2161 tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt cgggcgcgac
     2221 attggcgata tctacggctg gcaacctcgt agttatggcg aggagcgcct gcgtcttgat
     2281 gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag tggtgaaatt
     2341 gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg cttgtttggc
     2401 gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga tattcgtaaa
     2461 ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa agcggaaggc
     2521 attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg caaacatgtg
     2581 ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca gcacattcgt
     2641 tcgctcaaca tcaaaacgcc gggcgctgag caactgatca tgaatctctc aggcggaaat
     2701 cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat tttgctggat
     2761 gaacctacgc gcagcattga tgttggtgct aagcacgaaa tatataacgt aatttatgcg
     2821 ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga agtcctcggc
     2881 gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt gttacacgag
     2941 caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca ggcagttgcc
     3001 tgagtaagga gagtatgatg tcttctgttt ctacatcggg ttctggcgca cctaagtcgt
     3061 cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt gcggtgctct
     3121 ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg aaagggttgg
     3181 gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc cttgcttccg
     3241 gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc acggcggtgg
     3301 ttatcaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg cttggcattc
     3361 tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct ctgatcacaa
     3421 cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac ggtaaagcgg
     3481 tcggtatcga agatgaaagc ttctttgccc ttggttacgc taactggttc ggtctgcctg
     3541 cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgttgctg aataaaacca
     3601 cct
//
LOCUS       DAZSBF010000321         3601 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZSBF010000321 DAZSBF010000000
VERSION     DAZSBF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.326"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHXWD_004103"
     CDS             <1..173
                     /locus_tag="ACHXWD_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR7622832.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHXWD_004104"
     CDS             complement(345..674)
                     /locus_tag="ACHXWD_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR7622833.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHXWD_004105"
     CDS             complement(679..864)
                     /locus_tag="ACHXWD_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR7622834.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHXWD_004106"
     CDS             complement(861..3500)
                     /locus_tag="ACHXWD_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR7622835.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
BASE COUNT          875 a          970 c          819 g          937 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 g
//
LOCUS       DAZSBF010000322         3584 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZSBF010000322 DAZSBF010000000
VERSION     DAZSBF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.327"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            63..387
                     /locus_tag="ACHXWD_004107"
                     /pseudo
     CDS             63..387
                     /locus_tag="ACHXWD_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(417..3329)
                     /locus_tag="ACHXWD_004108"
     CDS             complement(417..3329)
                     /locus_tag="ACHXWD_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFR7622836.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPKGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            complement(3386..>3584)
                     /locus_tag="ACHXWD_004109"
     CDS             complement(3386..>3584)
                     /locus_tag="ACHXWD_004109"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622837.1"
                     /translation="GDMFTSEQHRCSNEKKEFQRRVYLRGDAFINLYTNFYSGIMTGL
                     QLLRHFQRIALPAYHPTFFIP"
BASE COUNT          898 a          963 c          882 g          841 t
ORIGIN      
        1 ggcaaaggcc atgaaagccc tgatttccgg aagcgagcga acagtccaca aactgttgaa
       61 cgtggtgtca gcggcggaca gggataagcc ctcgtcaaca gttccgtcag cactgcgaca
      121 gcgttcttta cccggtcaaa acagcgttgc ggtgcaccac gcctgacgga tgaactgcgt
      181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
      241 agggcaaagg cctcccggaa gttcagcccg gtcagttacc gcgcacatgg cctgcccgtg
      301 tcagaaaatc tgctggagca ggatttttac gccagtggtc cgaaccagaa gtgggcggga
      361 gacatcacgt acttacgtac agatgaagtt cggttacatc ctgtcagcac agaacctcac
      421 gctttttgag cggcacgacg gatccccccc acccagtatc cgacagactt ttcactgcgt
      481 tcgtaaaaac ccttcttccc tcccagcata tccactaatt ccggtgacat atcctgcaga
      541 taagtctgca aatcatgccc cagatcttcc ggtgactgca actcataaac cgcgcgccac
      601 acatcaccct gccttatttt aagccccggg ttaagaatat actgtccgtg gttcagcctc
      661 agaaaaagct tttttccata aggatttttg ctgttaactt catgttgaga gagctggctg
      721 ctgatataac tccttcgttt gcggcgttcc ggcagcaacg cttcaggaag atgaaccaca
      781 cactctgcca gtcgtgccgc actgaaggct tccgaaaaaa acagagcact tcccagatgc
      841 gtgtggaaca gcgcctgcat caggatcacc agaaacattg ccatctgccg gttgtccagt
      901 ttgatgaggc gtccttccac ctgcagtgac agactttcag gtttcagcag ggtatacaac
      961 tgcgcacttt tctgttgcgc ataacggggc gtactgaccg ccgctgacag aacttgatga
     1021 taagccgcca gcccgtcatt accggtcagc aagggatctg caccacgctc cagcagtaac
     1081 tgaaccagtg caatattacc tgcccgggct gctgacatca gcggagtaag attaaacggc
     1141 gaacggtgat caacaccata acgttcaata tcccgcaaca cggcaaccgg gttattcatt
     1201 gagtatggcc ggaaatactt cagctccagt aactgcagag tcttcgttct gggctgcctt
     1261 gtgggcttaa aatcttcccg gtacagtgca ttatacagcg ggaacaggga ataaatcaga
     1321 ctgtactcca gcgcctgcag ttgtatggtt ttatcggctg tacctgcgag gatatgctgt
     1381 ttccactgtc gtgcatcctc tgcggtgata atctgccatg gcatctcaga tgtctgcagg
     1441 atccggctac ggatggtgtc cgcctgctcc tgcttcccct gtttttcaag cagatgtgcg
     1501 gttttctgcc actcatcacg gctggaaatc tcttcctgca gattcagagg ctcttcctga
     1561 tgcgtgagcg caagcagact ccacagagga tgctccacct gctgttcaac aagatacaca
     1621 ttgtgtgtag cacgggtgag cgcaacatac agcgcgttgg taaaaaattt atagatttcc
     1681 gctgaacgat cctgcctgtc ccgcggacgg gaataacggg cctcgccctc aagatcagcc
     1741 ggtgtaagac cctcgcaaat atcatcaaat gcctgccggg cagcgcttac tatattgtaa
     1801 aggatcaccg tttcgtattc cagcccctta acctcctgaa ccgaaaagac cagcggcgta
     1861 ctgaaccagc atcgcgcctg tgctttctgt tccggatgca tcacaatgac cgctacacga
     1921 ttcgaaagtg aggttttggc attcagttcc tgctttgtct cttccctgtc gtccagcaac
     1981 cggatggtcc cttctgcctg accgcagctt ctgacaaaat ggtgactttc ccgatcaata
     2041 gcactaaagc ggacctgttt gagtctcagc aaccggttag ccagtgcagt gacgtggtgc
     2101 ccgttacggt aattcgcctg caggatatta acggttgtgt cgtttccctg ttgctgccgg
     2161 aaaaagagac ttttgagcga actccatgag aaaaagctcg gatgaactat ctgattggca
     2221 tcaccacaga gtaaaaactg accgggatga cgtagcgttt tcagtaccag ttgcagttga
     2281 acaggcgtca tatcctgaac ttcatcaaca aaaatgacat cataacgggg agttgccaac
     2341 gaaagatact gatgactgag cagattactg tcatactgat gcgactgttt cagccatgcg
     2401 atataacgtt caaacaggac atagaccgtc gggcggtcct ccatgccata gagcgactgc
     2461 cgtatcccca gtgacagata cgcctcccgg gacagcggcc cattcccgga ggcaaccgca
     2521 ccgatcacgc ccctgaactc ctcatacagg gtatgggcag cccccagtgc ggcgatagcc
     2581 ctgttgcggg gcagccagtc gctgaacgca ctgaaagtca cctccctgcc tttcggaatg
     2641 cgcagagttt ccagaaattc cgtcagcgaa agaaagtcaa tattctgtac ctcactccct
     2701 tcccctgaag cgtcatagag agtacgcgct ttttccacca gaaatgagga aaggctgaga
     2761 tacagtatat caccagcagc ctgcttcatt ttttccagta ccagcgccgt cttaccactt
     2821 cccgccgagc caacaatcac cagcggtagc gggtgttcgt acagtgcctg ctgatcatca
     2881 tcaaaggaca gcattttatc caggcggtgg aaacgcccat gagacggatt gatatacgtc
     2941 agcgcttcag tcgcgatatc aacaggatcc ggcactggtt gttgttgtaa gcgcccttca
     3001 tcaatcacca cgtttctgcg aagaaaacgt gaagtgttat aggcatgatt gcgaatatat
     3061 tccagaacaa ggcagatagt ttcaccgtgg tagcgataca gcgaaaacag taatcggtcc
     3121 cggatactga gccgggcgcg gtaaagattg gtgtcaattt tacgaacatc agcctgaaca
     3181 aagttatcgg tggcaatagc ctgtgcaaat tttttataac ccgggatcgt gtcaggattc
     3241 agagtatgat aaagacagat tttcatggct gccggtttcc gtcgtgcaaa aaacatgctg
     3301 attttacgaa ggaatgcctt actgaacaac cttgcccgga ataccccccg aaatcagatg
     3361 gaacaatcag aaaaatgctc tgcagttatg gaataaagaa ggtggggtgg tacgccggga
     3421 gggcgatccg ctggaaatgg cgcagtagct gcaggcctgt catgatgccg gaatagaagt
     3481 tcgtgtacag gttaatgaaa gcgtcacccc gtaaataaac tctgcgctga aattcctttt
     3541 tttcattgga gcacctgtgt tgttctgagg tgaacatatc acct
//
LOCUS       DAZSBF010000323         3577 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZSBF010000323 DAZSBF010000000
VERSION     DAZSBF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.328"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHXWD_004110"
     CDS             <1..196
                     /locus_tag="ACHXWD_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622838.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHXWD_004111"
     CDS             207..374
                     /locus_tag="ACHXWD_004111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622839.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHXWD_004112"
     CDS             371..715
                     /locus_tag="ACHXWD_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622840.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHXWD_004113"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHXWD_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR7622841.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1469
                     /locus_tag="ACHXWD_004114"
                     /pseudo
     CDS             1383..1469
                     /locus_tag="ACHXWD_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1466..1738
                     /locus_tag="ACHXWD_004115"
     CDS             1466..1738
                     /locus_tag="ACHXWD_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622842.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1740..2798
                     /locus_tag="ACHXWD_004116"
     CDS             1740..2798
                     /locus_tag="ACHXWD_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR7622843.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2799..3179
                     /locus_tag="ACHXWD_004117"
     CDS             2799..3179
                     /locus_tag="ACHXWD_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR7622844.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3176..>3577
                     /locus_tag="ACHXWD_004118"
     CDS             3176..>3577
                     /locus_tag="ACHXWD_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7622845.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          808 c          980 g          821 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgaggaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc ttccagacta cacaattctg ataattcagc
     1501 cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt ggtaccagca
     1561 tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca ggcgcggatg
     1621 cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg tcaggctccc
     1681 ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct ggaggtaatg
     1741 tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc gttaagccag
     1801 gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg gaaccaaaaa
     1861 gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg gtggaagaca
     1921 aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt ggtgctggtg
     1981 cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac ggcgattatc
     2041 atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg tgctggcact
     2101 gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct catcaaaacc
     2161 tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag gagcgtgaat
     2221 tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac gcgctaccgg
     2281 aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct gtgtacagtg
     2341 aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag cgcacaaaaa
     2401 atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa aagacggtgg
     2461 tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag aaatcacaac
     2521 gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg acgtgtggca
     2581 atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg ggaacaaagg
     2641 ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta catgccggag
     2701 taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt tttttaatgc
     2761 acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc atagaatttg
     2821 ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc acatattttg
     2881 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcggc
     2941 tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca ccggataagc
     3001 gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
     3061 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
     3121 gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc aaaaaatgaa
     3181 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
     3241 ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
     3301 gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
     3361 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
     3421 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
     3481 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gctgtcacgg
     3541 aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZSBF010000324         3559 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZSBF010000324 DAZSBF010000000
VERSION     DAZSBF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.329"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHXWD_004119"
     CDS             129..1376
                     /locus_tag="ACHXWD_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR7622846.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHXWD_004120"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHXWD_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR7622847.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHXWD_004121"
     CDS             complement(2228..2572)
                     /locus_tag="ACHXWD_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622848.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHXWD_004122"
     CDS             2814..3416
                     /locus_tag="ACHXWD_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622849.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          886 a          714 c          817 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct agggttgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttgaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggagagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgt
//
LOCUS       DAZSBF010000325         3498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZSBF010000325 DAZSBF010000000
VERSION     DAZSBF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.330"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..293
                     /locus_tag="ACHXWD_004123"
     CDS             <1..293
                     /locus_tag="ACHXWD_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR7622850.1"
                     /translation="RPRSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLR
                     PERRRRDILLEEVLRQAGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            533..793
                     /locus_tag="ACHXWD_004124"
     CDS             533..793
                     /locus_tag="ACHXWD_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFR7622851.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1017..1091
                     /gene="tap"
                     /locus_tag="ACHXWD_004125"
     CDS             1017..1091
                     /gene="tap"
                     /locus_tag="ACHXWD_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFR7622852.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1084..1941
                     /gene="repA"
                     /locus_tag="ACHXWD_004126"
     CDS             1084..1941
                     /gene="repA"
                     /locus_tag="ACHXWD_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFR7622853.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESDAGTLSITRATRALTFLAELGLITYQTEYDPLIGCNIPTDITFTPALFAALDV
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            2641..2832
                     /locus_tag="ACHXWD_004127"
     CDS             2641..2832
                     /locus_tag="ACHXWD_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622854.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTAACRLT
                     CRWRGIRIIQAAFNAFAKV"
     gene            2905..3156
                     /locus_tag="ACHXWD_004128"
     CDS             2905..3156
                     /locus_tag="ACHXWD_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFR7622855.1"
                     /translation="MPNIILSETSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            3153..3440
                     /locus_tag="ACHXWD_004129"
     CDS             3153..3440
                     /locus_tag="ACHXWD_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR7622856.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          930 a          859 c          886 g          823 t
ORIGIN      
        1 tccggccacg atcacgccag tggtggcagt tattcaggat ggtgtctcag tggcatgttg
       61 atgtggttat cgttgagctg cgttcgttca gtattgttgc agcagtagag ctggatgatg
      121 ccagccattt acgaccggaa cgcagacgcc gggatattct tctggaagag gttctgaggc
      181 aggctggtat tccgttgctc agaagccacg atgccagaaa actgctgcag atgaccggag
      241 aatggctgaa tacaacaggg gctgatcagc agtcaccgga acatcgtagc tgacgccttc
      301 gcgttgctta gttgtccaac cccggaaacg ggaaaaagca agttttcctc gctcccggcg
      361 tttccataac tgaaaaccat actatttcac agtttaaatc acattaaacg acagtaatcc
      421 ccgttgattt gtgcgccaac acagatcttc gccacaattc tcaagtcgct gatttcaaaa
      481 aactgtagta tcctctgcga aacgatccct gtttgagtat tgaggaggcg agatgtcgca
      541 gacagaaaat gcagtgactt cctcattgag tcaaaagcgg tttgtgcgca gaggtaagcc
      601 tatgactgac tctgagaaac aaatggccgc agttgcaaga aaacgtctta cacacaaaga
      661 gataaaagtt tttgtcaaaa atcctctgaa agatctcatg gttgagtact gcgagagaga
      721 ggggataaca caggctcagt tcgttgagaa aatcatcaaa gatgaactgc agagactgga
      781 tatactaaag taaagacttt actttgtggc gtagcatgct agattactga tcgtttaagg
      841 aattttatgg ctggccacgc cataaggtgg cagggaactg gttctgatgt ggatgtacag
      901 gagccagaaa agcaaaaacc ccgataatct tctttaactt tggcgagtca gaaagattac
      961 cggggcccac ttaaaccgta tagccaacaa tcaagctatg cggggagtat agttatatgc
     1021 ccggaaaagt tcaagacttc tttctgtgct cgctccttct gcgcattgta agtgcaggat
     1081 ggtgtgactg atcttcaaca aacttattac cgccaggtaa agaacccgaa tccggtgttc
     1141 acaccccgtg aaggtgcccg gacgcttccg ttctgcggga aactgatgga aaaggcggtg
     1201 ggcttcacct cccgttttga tttcgccatt catgtggcac atgcccgttc gcttggtctg
     1261 cgtcggcgca tgccaccggt actacgtcgc cgggccattg atgcgctgct gcaggggctg
     1321 tgtttccact atgacccgct ggccaaccgc gttcagtgct ccatcaccac gctggccatt
     1381 gagtgcggac tggcgacgga gtctgatgcc ggaacacttt ccatcacccg tgccacgcgt
     1441 gccctgacgt tcctggcaga gctggggctg attacctacc agacggaata tgacccgctt
     1501 atcggctgca acattccgac cgacatcacg ttcacaccgg cgctgtttgc cgccctcgat
     1561 gtatcagagg aggcggtggc gtccgcgcgc cgcagtcgtg tggaatggga aaacagacag
     1621 cgcaagaagc agggactgga taccctgggt atggatgaac tgatggcgaa agcctggcgt
     1681 tttgtgcgtg agcgtttccg cagttaccag acagagctta agtcccgtgg aatgaagcgt
     1741 gcccgtgcgc gtcgtgatgc ggacaggcag cgtcaggata ttgtcaccct ggtgaaacgg
     1801 caactgacgc gtgaaatctc ggaagggcgc ttcactgcca gtcgtgaggc ggtaaaacgt
     1861 gaagtggagc gtcgtgtgaa ggagcgcatg attctgtcac gtaaccgtaa ttacagccgg
     1921 ctggccacag cttccccctg aaagtgacct cctcagaata atccggcacg cgccggaggc
     1981 attcgcacgc ctgaagcccg ccagtgcaca aaaaatcagc gctacataca aaaaacaacc
     2041 tcatcatcca ccttctggtg catccggtcc cccctgtttt tgatacaaaa cacgcctcac
     2101 agacggggaa ttttgcttat ccacattaaa ctgcaaggga cttccccata aggttacaac
     2161 cgttcatgtc ataaagcgcc agccgttagc tttacagggt gcaatgtatc ttttaaacag
     2221 ctgtttatat ctcctttaaa ctacttaatt acatccattt aaaaagaaaa cctgttcact
     2281 gcctgtcctg tggacagaca gatatgcacc tcccaccgca agcggcgggc cccgaccgaa
     2341 gccgatttag ttacaacatg caaacataaa cccctaaaaa acccaagtcc gacgcagaac
     2401 tgaaaccaca acgcccctcc tcataactga aaagcgccgc tgcccccgcc cgaagggcgg
     2461 gaacagagtc gcttttaatt atgaatgttg taactacatc gtcatcgctg tcagtcttct
     2521 ggctggacgt atcgaataca cgctcgtaag cggccctgac ggcccgctaa cgcggagata
     2581 cgccccgact tcgggtaaac cctcgtcggg accactccga ccgcgcacag aagctctgtc
     2641 atggctgaaa acgggtatgg cttagcaggg ctggggatgg gtaaggtgaa atctgtcaat
     2701 cagtaccggc taacgccggg cttcggcggt tttactccgg tatcacatgt aaccgcagcg
     2761 tgccgcctta catgccgctg gcgcggcatc agaataatac aagcagcatt taatgcattt
     2821 gcaaaagtgt gagtaataaa gacttgttaa gtggtcgttt cagggatatc gttaagactt
     2881 gttaaacagt ctcaaaggtg cgttatgcct aacatcattc tgtctgagac cagtgcaagt
     2941 gtcagtgaac tgaaaaaaaa tcctatggct acagtcagtg ccggagatgg ttaccctgtc
     3001 gccattctta accggaatca gccggcattc tactgtgtgc ctgctgaact gtatgaaagg
     3061 atgcttgatg ctctggatga ccaggaactg gtgaaactgg ttactgaacg cagcaatcag
     3121 ccactgcatg atgtggacct ggacagttat ttatgactta tacggtaaaa ttcagggatg
     3181 atgcgctcaa ggaatggctg aaactggata agaccattca acaacagttt gtgaaaaaac
     3241 taaaaaaatg cagcgaaaat cctcatatac cgtcggcaaa actaagaggg ctaaaggact
     3301 gctacaaaat taaactacgt gcatcgggat ttcgtctggt ttatcaggtg attgacgata
     3361 tgttaattat tgcggttgtt gcggtaggaa aacgagaacg tagtaacgtt tataatctcg
     3421 ccagtgagag aatgagatag taacatataa ataacctttc ccgcagataa ctcagaattg
     3481 ttattttctc tgtggact
//
LOCUS       DAZSBF010000326         3487 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZSBF010000326 DAZSBF010000000
VERSION     DAZSBF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.331"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            70..738
                     /locus_tag="ACHXWD_004130"
     CDS             70..738
                     /locus_tag="ACHXWD_004130"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR7622857.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(683..820)
                     /locus_tag="ACHXWD_004131"
     CDS             complement(683..820)
                     /locus_tag="ACHXWD_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR7622858.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(793..891)
                     /locus_tag="ACHXWD_004132"
                     /pseudo
     CDS             complement(793..891)
                     /locus_tag="ACHXWD_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(880..1119)
                     /locus_tag="ACHXWD_004133"
                     /pseudo
     CDS             complement(880..1119)
                     /locus_tag="ACHXWD_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1218..2108
                     /locus_tag="ACHXWD_004134"
                     /pseudo
     CDS             1218..2108
                     /locus_tag="ACHXWD_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2111..2644
                     /locus_tag="ACHXWD_004135"
     CDS             2111..2644
                     /locus_tag="ACHXWD_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7622859.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2673..3200)
                     /locus_tag="ACHXWD_004136"
     CDS             complement(2673..3200)
                     /locus_tag="ACHXWD_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7622860.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3202..>3487)
                     /locus_tag="ACHXWD_004137"
     CDS             complement(3202..>3487)
                     /locus_tag="ACHXWD_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR7622861.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          994 a          771 c          752 g          970 t
ORIGIN      
        1 gctgcggcag catttaaccc cttgtaattc atcgccataa ttgatttaat tcacaaataa
       61 aactataaca tggtgaaatc aatgaaaaca aacacagatg atggggctaa aatttacaca
      121 ccacttaccc taaagcttta tgactggtgg gttttgggag tatcaaatcg gcttgcatgg
      181 ggatgtccta caaaggaaca ccttcttcca cactttctgg aacatttagg taacaaccat
      241 ctggatattg gtgttggaac tgggttttac cttactcacg tacctgagag tagtctgata
      301 tctttaatgg atttgaacga agctagcctg aacgcggcat ctacaagggc tggggaatca
      361 aaaattaaac ataaaattag ccatgatgtt tttgaacctt atcccgcggc gttacatggt
      421 caatttgatt ccatttccat gttttacctt cttcactgcc tgcctggaaa tatatctaca
      481 aaaagctgtg taatacgcaa tgcggcgcag gccttaactg acgatggaac tctatacgga
      541 gccacaattc ttggcgatgg agttgtgcac aatagcttcg gtcaaaaact gatgcgcatt
      601 tacaatcaga aaggcatctt ttcaaacaca aaagattccg aagaaggctt aacacatata
      661 ctctcagagc atttcgagaa tgttaaaacc agggttcaag gtactgtagt aatgttttcc
      721 gcttcaggga aaaaatagca tccaaccgca gcacgttctt gcttaagacg tgctgcggca
      781 taatcccaat gattactccc tgacagggtt cgtaggccac tcaatatcag gtgcagttga
      841 tgtatcaaca cggttcagca acacccgata ctttttctag gcttccacca ccagcacgac
      901 aagatgccgc atacagtgcc ccagtcagtc cagttttcag acaaccagtg cgtcaccttt
      961 ttgaaggcgc tttaaagcac gttttaatcc aggtcggcct gtccttgttc cgcttaattt
     1021 atcttcaaat atttgttcat atcctgcaca aacaagagcg tttcgttgca ggtctgtatt
     1081 ctggtcattt gttgataccc ttacatagcc aatcagcacg ctgaatctcc cgtccaaaag
     1141 cacaaatcat gccatgcagg ccagaaaccg ccattatcta aaacctcggt ttacgagaac
     1201 tcggcacaag cggggagaaa ataccgttac tcagtacagc gaatacctgg actaatcgac
     1261 aaacattcag cggtggcctt tctggtgaac tatccggcaa tgcttctaca gctgcaaaat
     1321 taaaaactgc caggaaaata agcaatgtgg cttttgatgg ttcctccgat atcacattaa
     1381 aagcaagtca tgttggtgcg tttgccttag ggaaaacagg aagcaccgtt gcgaatgata
     1441 aagcagttgg atggaactgg agtagcggag cctataacgc aactattagt ggtgcatcaa
     1501 cgttaattat tcatttttat atgggagaag gaagttgtcc tgcagctcag tttcggatta
     1561 attataaaaa tggcggtatt ttttatcgtt cagcccgtga tggttatggt tttgaagccg
     1621 actggtccga attttacacc accagaaaac cttcagcagg agatgttggt gcactgccgt
     1681 tatctggtgg tcaactgaat ggtgcactgg gtatcggaac atccagtgct cttggcggta
     1741 attcgattgt attgggtgat aatgacacgg gctttaaaca aaaaggcgat ggtaatctgg
     1801 atgtttatgc taataacgtc catgttatgc gctttgtctc cggaagcatt caaagtaata
     1861 aaaccataaa tattacggga cgtgttaatc cctcggatta cggtaacttt gattcccgct
     1921 atgtgagaga tgtcagactt ggcacacgag ttgtttagac catgaagaaa ggcgtgatgt
     1981 atgaaaaatc aggccatgta atcacggggc ttggtattat cggtgaagtc gatggtgatg
     2041 atccggcagt attcagacca atacaaaagt taattaacgg aacatggtat aacgtatcgc
     2101 aggtataatc atgcagcatt taaaaaacat tgtcgcaggt aatccaaaaa ccgttgaaca
     2161 ataccagcta acaaagaatt ttgatgttat ctggttatgg tccgaagatg gaaaaaattg
     2221 gcatgaggaa ttaaaaaact ttcaggagga tacaataaaa ctggcttaca cagttgaggg
     2281 aataattatt gctatagaca aggatgtatc agcaattaat ccagaaggtt taagtgtcgt
     2341 tgagttgcct gatattacag caaatcgccg ggctgatatt tcgggcaact ggatgttcaa
     2401 agatggtgcg gtgataaagc gaacttatac cgaggaagag caaaggcaac aagcggaaaa
     2461 tgaaaagcag agcctgctgc agctcgtcag ggataaaacc cagctgtggg actctcagct
     2521 acagctgggc atcatttccg atgagaataa acaaaaatta accgagtgga tgctctatgc
     2581 gcagaaggtt gaatccacag acacctccag cctgccagta acgtttcccg aacaaccaga
     2641 atgagagaag gcccgctatc gggccttaat ttttattcag gcttttgtgg ccattcagga
     2701 tttgccgtat ccacacggct gaccagaaca ctgtagcgtt cccatgcttc cagtcgtgtg
     2761 cgttcctcgt ctgttgccat attcagcctg acagctcgtt ccagcggctg gatgactgat
     2821 tcagcttcgg aaagcaatgc ggccttttgt gattcggcct gttgttgcag ttcctgttct
     2881 gtatattccc tctttactac tttaccatcc agaaacatcc attttccgtt attgtctgcg
     2941 tgacgatttg ccgtaatatc aggaacttca actatgctta gcccttcagg attaatagtt
     3001 gaaacgtctt tactgacagc aacgatgatg ttgttctcat cgtaagcaat ttttatggtg
     3061 tctggctgaa aattcttttg ttcctcatac cagtttttgc catcttcgca aaagagccat
     3121 accacgcccg cttttttagt tagctggtat tgttccggtg ttttcggatt cccggcctta
     3181 atatttttca ggtgcatcat cttaaacact ccccacgttg taccatattc ccccaatcaa
     3241 tttctgaatt ggccttctgg caacagtgtc gacaatatca tccgcattgc tgttaattgc
     3301 ggctgttaaa acataacctg acgcatcgcc aaagccgtag tcgcgccata cgtttttata
     3361 ttcaatgctg ccaagacgaa tatcccgaac gacatgatta tgcagccatg tgcttaacca
     3421 gttgttttcc cacactgaac cataaatatc gccactagac gcaacttgcg caccacttcc
     3481 cagcgca
//
LOCUS       DAZSBF010000327         3427 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZSBF010000327 DAZSBF010000000
VERSION     DAZSBF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.332"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            138..1103
                     /gene="dusB"
                     /locus_tag="ACHXWD_004138"
     CDS             138..1103
                     /gene="dusB"
                     /locus_tag="ACHXWD_004138"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR7622862.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1129..1425
                     /gene="fis"
                     /locus_tag="ACHXWD_004139"
     CDS             1129..1425
                     /gene="fis"
                     /locus_tag="ACHXWD_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR7622863.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHXWD_004140"
     CDS             1511..2395
                     /gene="yhdJ"
                     /locus_tag="ACHXWD_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR7622864.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2479..2658
                     /locus_tag="ACHXWD_004141"
     CDS             2479..2658
                     /locus_tag="ACHXWD_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR7622865.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACHXWD_004142"
     CDS             complement(2661..3323)
                     /gene="acrS"
                     /locus_tag="ACHXWD_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR7622866.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          939 a          789 c          810 g          889 t
ORIGIN      
        1 attatctcac cacgctcatt ttaaatgcaa ttctttgatc catctcagag gattggtcaa
       61 agtttggcct ttcatctcgt gcaaaaaatg cgtaatatac gccgccttgc agtcacagta
      121 tggtcatttc ttaactcatg cgcatcggac aatatcagct cagaaatcgc ctgatcgcag
      181 cgcccatggc tggcattaca gacagacctt ttcggacgtt gtgctacgag atgggagccg
      241 gattgacagt atccgagatg atgtcttcta acccacaggt ttgggaaagc gacaaatctc
      301 gtttacggat ggtgcacatt gatgaacccg gtattcgcac cgtgcaaatt gctggtagcg
      361 atccgaaaga aatggcagat gcagcacgta ttaacgtgga aagcggtgcc cagattattg
      421 atatcaatat gggttgcccg gctaaaaaag tgaatcgcaa gctcgcaggt tcagccctct
      481 tgcagtaccc ggatgtcgtt aaatcgatcc ttaccgaggt cgtcaatgca gtggacgttc
      541 ctgttaccct gaagattcgc accggctggg caccggaaca ccgtaactgc gaagagattg
      601 cccaactggc tgaagactgt ggcattcagg ctctgaccat tcatggccgt acacgcgcct
      661 gtttgttcaa tggagaagct gagtacgaca gtattcgggc agttaagcag aaagtttcca
      721 ttccggttat cgcgaatggc gacattactg acccgcttaa agccagagct gtgctcgact
      781 atacaggggc ggatgccctg atgataggcc gcgcagctca gggaagaccc tggatctttc
      841 gggaaatcca gcattatctg gacactgggg agttgctgcc cccgctgcct ttggcagagg
      901 ttaagcgctt gctttgcgcg cacgttcggg aactgcatga cttttatggt ccggcaaaag
      961 ggtaccgaat tgcacgtaaa cacgtttcct ggtatctcca ggaacacgct ccaaatgacc
     1021 agtttcggcg cacattcaac gccattgagg atgccagcga acagctggag gcgttggagg
     1081 catacttcga aaattttgcg taaacagaaa taaagagctg acagaactat gttcgaacaa
     1141 cgcgtaaatt ctgacgtact gaccgtttct accgttaact ctcaggatca ggtaacccaa
     1201 aaacccctgc gtgactcggt taaacaggca ctgaagaact attttgctca actgaatggt
     1261 caggatgtga atgacctcta tgagctggta ctggctgaag tagaacagcc cctgttggac
     1321 atggtgatgc aatacacccg tggtaaccag acccgtgctg cgctgatgat gggcatcaac
     1381 cgtggtacgc tgcgtaaaaa attgaaaaaa tacggcatga actaattcag gttagctaaa
     1441 tgcttgatta aaaaggcgct actcggcatg gggaagcgcc ttttttatag gtgtcacaaa
     1501 gggagtgacc atgagaacag gatgtgaacc gacccggttt ggtaatgaag ctaagaccat
     1561 tattcacggt gatgcccttg ccgaacttaa aaagctacct actgaaagcg tcgatctgat
     1621 ctttgccgac ccaccgtata acatcggtaa aaattttgat ggtctgatcg aagcctggaa
     1681 agaagatctg tttatcgact ggctgtttga agtgattgta gagtgccacc gcgttctgaa
     1741 aaagcagggc agcatgtaca ttatgaacag tacggaaaac atgcccttta tcgatctcca
     1801 gtgccgcaag ctttttacca tcaaaagtcg catcgtctgg tcatatgaca gttctggagt
     1861 acaggcgaaa aaacactacg gctccatgta cgaacccatc ctgatgatgg tgaaagacgc
     1921 aaagaactac acattcaacg gtgatgctat tctggtagaa gccaaaaccg gatcgcagcg
     1981 cgcgttgatc gattatcgca aaaatcctcc acagccatac aatcatcaaa aagtaccggg
     2041 taacgtctgg gattttccgc gcgtgcgtta tttgatggat gaatatgaga accacccgac
     2101 gcaaaaaccg gaagccttac tgaaacgcat tattctcgcc tcttccaacc caggcgatat
     2161 cgttctcgac ccgtttgccg gtagctttac taccggtgcc gtagccatcg ccagcggacg
     2221 aaaattcatt ggtattgaga tcaacagcga gtacatcaaa atggggcttc gacggctgga
     2281 tgtcgcgtcg cattactccg cggaagaact ggcgaaagtg aaaaaaagaa agaccggcaa
     2341 cctgtcaaaa cgaagccggt taagcgaagt tgaccccgat ctcattgcaa agtaaaggaa
     2401 gtgtaagcct ggttttcaga ttattcattt cgtgtatatt tcctgccaga cttggttaaa
     2461 catgcacagg ctcaggtaat gattcgcaag tattggtggc tcgtcgtttt cgctgtcttc
     2521 gtttttctgt ttgatacttt actgatgcag tggattgaac tgctgacaac agaaacagac
     2581 aaatgccgta atatgaactc agtttatcca ctaaaactgg ttaactgtga cgaactgaat
     2641 tttcaggaca gaatgtgaat ttacatgaca cttaattcat ttgtttgatg aattaatttc
     2701 gttatgtttt catctggcat gaacattctt aatacgttat cgaccagcgc gggtgcttgt
     2761 ttataaagat cataacccgc catattcatt aaccagtttt gaacaattcc gctgaaggca
     2821 ccatcaataa taatcatcac aacatctaaa tcgaggttat ttgctacaca accttgttgc
     2881 tgacacgcct gcaatacttc gcggagagtc tgcggattaa agcccatctt ttcgcgtatc
     2941 actccctcgg ccagcatccc atcattaaat tcacatttgt gatataagat tttcagcaac
     3001 gcctgctggc ggggaatttt ggcaatatat tgcaagccga caatcaattt ttcacgcaat
     3061 tgttgaaacg ggtcatgctc taatccagcc gtcaagtgtt cctggattaa ctcccgcaat
     3121 gaaggctgtt gcaaccacat ctcattaaac agttgggtct tgttctcgaa gtgccagtag
     3181 atagcgccac gcgtaacgtt agcggcatcg gcaatgtcgt tgaacgtcgt cttgcttacg
     3241 ccatgttgcg caaactgggc gatggcagtt tcaatcagtt cttgccgggt cttcagagct
     3301 tcggctttgg ttctttttgc catgattaat tattcaggaa ataaatatat tcgacacagg
     3361 gtgagaaaat agcgaaggtt aatctatcat ctaatgtgta tttatacgag agggtaacat
     3421 tgagttg
//
LOCUS       DAZSBF010000328         3403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZSBF010000328 DAZSBF010000000
VERSION     DAZSBF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.333"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            532..2385
                     /locus_tag="ACHXWD_004143"
     CDS             532..2385
                     /locus_tag="ACHXWD_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR7622867.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2536..2751
                     /locus_tag="ACHXWD_004144"
     CDS             2536..2751
                     /locus_tag="ACHXWD_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR7622868.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2756..3070
                     /locus_tag="ACHXWD_004145"
     CDS             2756..3070
                     /locus_tag="ACHXWD_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR7622869.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          842 a          817 c          931 g          813 t
ORIGIN      
        1 ttccaggccc atccgctccg gtgggccttt ttactgcaga aaacaggttc cccgttaaat
       61 gctatgttgc tcacaattca gtaagttgac agttgcctgt cagactgggc atttgttaaa
      121 aaaatttcgc atggtgaatc cccctgagcg gaggggcgac tggtgacggt ataatctctg
      181 attatcaaaa cgagaatgac gcgggtttag tggcaccggg ctgaactcac cgggaggcac
      241 ccggcaccat gtgcatgatg atacagatac gcggctttag cccctctccg gaggggtttt
      301 cttgtgggca aaaaaagccc gcgctgggag acgcgggcgg caaggaataa acaataaaac
      361 gtgaagtaat atttcagctg gcgaataata ccccatagta atcactctgc gcaactgcgc
      421 ggtctttttc gaattgcggg ctgtcgtctc tcttctgcca ttgtcctgta acttccggac
      481 ttcagcccgc tccttatttt actcacaata ttatcccggc cgggaggatt catggcattt
      541 aaacactatg acgtggtcag ggcggcatcg ccgtcagacc ttgcgaaacg actgacacaa
      601 aaactgaagg agggctggca gccgtttggt agtccggtgg ccataacccc ttataccctg
      661 atgcaggcga ttgcagcaga aggtgatgtg gtcgtcagtg gtgcaactga gccggagtgg
      721 tactacgtca tcgtactggc cgggcaatcc aatgccatgg cttacggtga aggtcttccg
      781 cttccggatt catacgatgc gccccatccg cgcattaagc aactggcccg tcgtaacaca
      841 gtgactcccg gtggtgaagt atgcgtattt aacgacatca ttcctgctga ccattgtctg
      901 catgatgttc aggatatgag tacgattaac catccccggg ctgacctgag caaagggcag
      961 tacggctgtg tcggacaggg cttacatatt gccaaaaaac tgcttccgta tatccctaat
     1021 aatgcgggga tcctgctggt accatgctgt cgtggtggtt cggcattcac ccagggcacg
     1081 gaggggacat tcagcgagtc cacgggggcc agtcaggatt cggctcgctg gggagtgggt
     1141 aagccgttat atcaggatct gcttttccgc acgaaggcag cattgcagaa aaacccgaaa
     1201 aacgttttgc tggcgatatg ctggatgcag ggggaattcg atatgacgaa tgccagttac
     1261 gcccagcagc cagcagcatt tcttgcaatg gtacagcagt tccgtgctga ccttgccggg
     1321 ctggcggcgc agtgtcacgg tggaagtccg gcatcagtcc cctggatttg tggcgacacg
     1381 acatacgcgt ggaaacaaga acacggtacg caatatgaag tggtatatgg tgcatataaa
     1441 ggtaaagaat cccagcagat ttattttgtt ccctttatga ccgatggtag cggagttaat
     1501 acaccgacaa acaacccgtc agaagatcct gatattgccg ggtctggtta ttacggttcg
     1561 gcatcccgaa cgaacaaaaa ctgggtatca tcaaatcgcc cgacgcattt cagctcatgg
     1621 gcgcgtcgtg gcattattcc cgatcgtatg gcaactgcta ttctgaacgt agccggtcgc
     1681 accttagcct tcattagtgg taaggcaccg gaaatcaaac cctcgcccgg cggcgacacg
     1741 ccatcggggc cgtctgaaga tgcatccata cgcacaatct ccctgttgcc gacagccgga
     1801 gacgctgctg cgcagggctg gagcattaag aatggcggag ttcagttgtc agatggtgta
     1861 tttaagatca ccaagcagag caataaagcc tggtccctga cgcgcccggt ggatgacgca
     1921 gtctccctgc tgacacgggg tggcagactg agctgtaagt ttcgactgtc aggcgcactg
     1981 accaacaacc agttcggtct gggaatttat ctgtataccg atgtagcgtt acctgacgtc
     2041 gtggcgatga ccgggactgg taacccgttc ctgatgtcgt tcttcaccca gaccacagac
     2101 ggcaaactga atctgatgca tcacaagaaa gcaggaaaca caaagttggg cgagttcggg
     2161 aattacagta acgactggct gacgctggag ctggtgttca ccgccggcag tgccacggtt
     2221 actccgaaac tgaatggagt ggctggcccg gcattccagg tcataaaaga cagtctgaca
     2281 ctggggctga atgcgctgac gctgacggat attaccaaaa atgcagcgta tggcgttgag
     2341 atagaaagtc tggtgctgga gataaatgca ccggcatcat cataaaaagt gagccagcca
     2401 aatggaaggt atcgttaaac tcaccggtag tgtcagtggg tcgtctgaga tgcctgcatg
     2461 agttatcaga gccatcagta cttaactggt ggcttttttt attgttgtca gcttccggat
     2521 aacgggagac ggggtatgta ccagatggaa aaaatcacaa caggtgtgtc atacaccacg
     2581 tcagcggtgg gaacgggcta ctggttcctg cagttgctgg acagggtttc cccgtctcag
     2641 tgggcggcaa taggcgtgct ggggagtctg ctgtttgggc tgctgacata tctgactaac
     2701 ctgtatttca aaatcagaga ggaccgtcgt aaggctgcac ggggagagta attcaatgac
     2761 tcaaaactat gaactgattg tgaaagggat ccgcaatttt gagaataaag ttacggtaac
     2821 tttagcgtta cgggacaaaa aacgctttga cggtgaaatt tttgacctgg acatctcgct
     2881 ggaccgtgtt gaaggtgccg cgctggagtt ttatgaggca gcagccagaa ggagcatcag
     2941 acaggtcttc ctggatgttg ctgccgggtt atgtgaaggg gacgagctgt tgccagaaac
     3001 gcgcccctgt tcagaggcgc ggtataccat aaaaattaac agttctgata actcgattac
     3061 gggttgttag ctttttgcag ttggctttcc agtatctttc attggtagca tcctgataaa
     3121 tatccatgag cgcaaaaatc aaatacggcc tgtcagctgc tgttctggcg ctgattgcaa
     3181 aatggcatct gaatcgatcg cagtttggca ttcgaatcga tcatgatttg gcatccatct
     3241 ccgatcacag tttggcatca caatcgatca cgatttggca tacttccgat cattgattgg
     3301 aattcgccag tcactccggg aattaactct tttcaccaca gtctccattg ccgtgtttaa
     3361 accagtggag acgtcaatgt ccaaaaagag aaaatccagg agc
//
LOCUS       DAZSBF010000329         3382 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZSBF010000329 DAZSBF010000000
VERSION     DAZSBF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.334"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHXWD_004146"
     CDS             complement(<1..312)
                     /locus_tag="ACHXWD_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR7622870.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHXWD_004147"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHXWD_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR7622871.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHXWD_004148"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHXWD_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR7622872.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          920 a          878 c          734 g          850 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccaaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc caaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atgtttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt at
//
LOCUS       DAZSBF010000330         3347 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZSBF010000330 DAZSBF010000000
VERSION     DAZSBF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.335"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1363)
                     /locus_tag="ACHXWD_004149"
     CDS             complement(<1..1363)
                     /locus_tag="ACHXWD_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR7622873.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1428..1547)
                     /locus_tag="ACHXWD_004150"
     CDS             complement(1428..1547)
                     /locus_tag="ACHXWD_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR7622874.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1771..1929)
                     /locus_tag="ACHXWD_004151"
                     /pseudo
     CDS             complement(1771..1929)
                     /locus_tag="ACHXWD_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2274..>3347)
                     /locus_tag="ACHXWD_004152"
     CDS             complement(2274..>3347)
                     /locus_tag="ACHXWD_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622875.1"
                     /translation="AVSETDLSSNKNNVDQFIECAKRNEQTLFDNIRKSDFHVDSFKP
                     GSTRSVILETQPNVCMEPHNLYDNQIDKVSPVTKNSQQVKGHYGDKFKEMQMFLNQMS
                     NALQQTPSLSESKEHTIDIQKITDAFVKEFRGVLIDKNGNCSERLFNFYECCYKFLPR
                     AQPQDKVESYNSALQAFSIFRSSTLNNNDVGFNFKLFPEVKLSGENLETVFKYKKGSF
                     VREIARINITLQKEEDGLYNLGGLDFKGCFFSGQNFSNYDIQYVNWRTSLFDLDTPCI
                     FNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE
                     DDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          923 a          759 c          609 g         1056 t
ORIGIN      
        1 caactaaagc actgcccgtc tcctggttaa ccccatgtgc tataccgccg gtatagacaa
       61 tcagcgagcc tccggcgcta atattgctgc cagttgccgt accatctttc tcaacaacct
      121 gccggctccc ggtggaaata tttgtcgtgt cagctttccc gccgctcttg atattttgtg
      181 ttccgctgtt gatattggta cccgtggcaa taccatgatt attaatattc tgtgtaccac
      241 cattgattat ggtattggtc gcgtttctgg taacatccat taccccgccg ttatctattc
      301 gggtcgcatc agctttagca ttcgttaaaa ctgacattgt tcctttatct ttaataatcg
      361 tcttatttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
      421 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
      481 taccgttagt gtttgttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
      541 caccactaac atcaagcacg ccaccggaat aaacttcaat aacatccgag gagctggtgt
      601 aatcaacaat ttgcgtacca ccagaataga tctgagtatt ttttgccgtt gacttattat
      661 tcagagactg agttccacct tcaatcgtcg tgtccagcgc atggctttca tatactcttt
      721 gctcaccgcc atttttaatg gtggttgttc ctactgtgct ctgttcaaca tactgtcgtc
      781 caccatttat gattgtgttc gttgccatac ttccttgcgt gatgtcctga taaccggact
      841 tatttatcgt tgtaccatca acatgcccct gagttgttac tgtctgccct ccaccatcaa
      901 tgatggttcc attcgcagtc cccccactta tgtgtgaatt accaccctta attgtcgttc
      961 cattgctgat accgcctgta taaacgtcct gattgccact gtcgattatc gtaccggtgg
     1021 aaatgccccc gtcatgaatt gtctgtcttc ctccgttaat ggttgtatta ttagactgcc
     1081 ccatataatt gttattcctt ccaacatctt gatatccacc agattcaata agacttccat
     1141 tagatactcc gccataaaca ctctgctggc catggttgat aatatgagtg ttatttgttg
     1201 tacctcgttc atctactcgt tggttgccat ctacagtctc atcgtttacc acaccaataa
     1261 cattagtagt aaaggccgcc atcacgggcg gggcatatat caaggcagat atcaataagg
     1321 aaagtactga gcggcgacaa taatggggac tggtcctgtt cataaatttc atcctctgaa
     1381 aagtgaatac tgagtagcgt ttaagcgact ttagctctgc tgcaacatca gcccgcaggc
     1441 accagaccgg ggggttcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     1501 aaagacatca tgatgaaatg atgatgttcc acataagaaa gaggcatttt ttaaacacag
     1561 tgcgctgaag tgtgattgga taaaaaagtc aatctattca ggaaatacgg gcgtattctt
     1621 ttctttcgac aatcaggccg tcggcaaaat aaaatgattt acataatcgt ttctgatgaa
     1681 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattc ttttactttt
     1741 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     1801 accacgtttg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     1861 gtgcggttta cagactaccc gtgcgtccac cgccgataat gtcctgagca gttttttcag
     1921 tccggacctc ctgccacggt tttacgttga agaggtaacc ctgagcacgc agttcttcag
     1981 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag
     2041 ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattta
     2101 aactcagtaa atcacagcac agatcaaggg gttacgtgaa agcgtagccc ctttttcttt
     2161 ggtagtggca gcaaaatggt tgtagtgtaa aaaataatcc cgtttaatca atcaataata
     2221 catattgttt caatctacgt tattatctct ttgtaaaaat agccatttat taatcattga
     2281 aaactgcttt tagaacttga tacaacggga ctagtcacaa caggactatt ctcaacggga
     2341 tcatcctcag aggaactatc atcaaagtca tcatccataa ataaaatatc atcgaatggt
     2401 gccacgcccg tgatgagttt tattttatta ttacgatcag tcaagactcc acttaaaccg
     2461 ttttcgctca caggttttaa tgatttttca ttactcttgt tgtaagcagg cgcattaaaa
     2521 atacacggag tatcaagatc aaacaatgac gttctccagt tcacatattg aatatcatag
     2581 ttactgaagt tctgtccaga aaagaagcat cccttaaaat ccaatccacc taaattatat
     2641 aaaccatcct cttctttttg gagagtaatg ttaattctgg ctatctcccg gacaaaactt
     2701 ccttttttgt atttgaatac cgtttcaaga ttttcaccag acagcttgac ttctgggaat
     2761 aatttaaaat taaaccctac atcattatta ttcaaagtag atgaacgaaa gatggaaaaa
     2821 gcttgtagtg ctgaattata gctttcgact ttatcttgag gctgcgctct tggtaaaaac
     2881 ttatagcagc actcataaaa attgaataaa cgttctgaac aattcccatt tttatcaatt
     2941 aatacccccc ggaattcttt cacaaaggca tccgttattt tttgaatatc tatggtgtgt
     3001 tcctttgact ctgacaaaga tggagtctgt tgaagtgcat tgctcatctg gttaaggaac
     3061 atctgcattt ctttaaattt atctccatag tggcctttaa cttgctgaga gtttttagtt
     3121 acaggtgaaa ccttatctat ttggttgtca tataaattat gtggttccat acagacattt
     3181 ggttgtgttt ctaaaataac actacgtgta ctacctggct taaatgaatc aacatgaaaa
     3241 tcactttttc ttatattatc gaatagggtc tgttcatttc tcttagcgca ttcaataaac
     3301 tgatcaacat tatttttatt cgatgacagg tcagtttcag aaaccgc
//
LOCUS       DAZSBF010000331         3318 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZSBF010000331 DAZSBF010000000
VERSION     DAZSBF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.336"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHXWD_004153"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHXWD_004153"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR7622876.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHXWD_004154"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHXWD_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR7622877.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHXWD_004155"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHXWD_004155"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR7622878.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHXWD_004156"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHXWD_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR7622879.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          785 a          878 c          783 g          872 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat
     3241 gacaaaggaa atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt
     3301 atttaaataa ttacccac
//
LOCUS       DAZSBF010000332         3316 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZSBF010000332 DAZSBF010000000
VERSION     DAZSBF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.337"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            119..547
                     /gene="uspC"
                     /locus_tag="ACHXWD_004157"
     CDS             119..547
                     /gene="uspC"
                     /locus_tag="ACHXWD_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR7622880.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(554..1978)
                     /gene="otsA"
                     /locus_tag="ACHXWD_004158"
     CDS             complement(554..1978)
                     /gene="otsA"
                     /locus_tag="ACHXWD_004158"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR7622881.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(1953..2753)
                     /gene="otsB"
                     /locus_tag="ACHXWD_004159"
     CDS             complement(1953..2753)
                     /gene="otsB"
                     /locus_tag="ACHXWD_004159"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR7622882.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(2920..>3316)
                     /gene="araH"
                     /locus_tag="ACHXWD_004160"
     CDS             complement(2920..>3316)
                     /gene="araH"
                     /locus_tag="ACHXWD_004160"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR7622883.1"
                     /translation="RNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAIAGIILAS
                     RMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENAMNLLNIS
                     PFAQYVVRGLILLAAVIFDRYKQKAKRIV"
BASE COUNT          836 a          833 c          785 g          862 t
ORIGIN      
        1 tgtctttact aataatcaga ctaatattta cctgtttgac cgagttggga ttgcgtcgtt
       61 tctccattag gagtaaagct ttaatgtcac ctgaagttca cagaataagg aacaggttat
      121 gagctatagc aatattcttg tcgctgttgc ggttacaccg gaaagtcagc aactgctggc
      181 aaaagcggta tctatcgcca ggccagtaaa gggacacatc agtttaatta ctctcgcttc
      241 cgacccggaa atgtacaatc aattagccgc gccgatgctt gaagatttac gcagcgtgat
      301 gcaagaagaa acgcaaagct ttcttgataa gttcattcag gatgcggggt atcccgttga
      361 caagacgttt attgcctacg gtgagttaag cgaacatatt ctggaagtat gtcacaaaca
      421 tcatttcgat ttggtgattt gcggtaatca caatcacagt ttcttttcgc gagcatcctg
      481 ctcggcgaaa agagtgattg cctcaagtga ggtcgatgtg ctgttagttc cacttacggg
      541 agattaaccg ctcctacgca agctttggaa aggtagcaac tttatcgcgc tgctggcttt
      601 ccgcgcttcg cggaactatc tgctttaggt cgctaatgaa gcactcctgc cagtggttaa
      661 tatcgttttt cacgataacg tccagcattt ctgcatgacg ggaaatacgt tccgccagcg
      721 acatagtcaa tgcacgatcc agcgcagctg caacttcgtc acgatcgtag gggttaacaa
      781 ttaacgccga cgttaactcg tttgccgctc ccgcaaattg cgaaagaaca agaacgcccg
      841 ggttggctgg gtcctgagca gcaacatact cttttgctac caggttcatc ccgtcacgca
      901 gtggcgtcac taagcccacg tcagagtagc ggaatatttt catcagtaat ttacggtcaa
      961 aatgctgatt caaataataa agcggcgtcc agcctaattg cccgtattta ccattaattc
     1021 gtccagcttc attttcgagc tgatgacgaa tatcctgata ggcttgcaca tcaccacgcg
     1081 acgttggtgc aatctgggta taacgaattt taccatgatg ctgcggatat ttttccagca
     1141 acgcttcata ggcgagaaaa cgctctggca aacctttgga ataatccagc cgttcgacag
     1201 aaaagatatt ttgtacgttt ttcagttccg ctttaagttg cgccagtttt ggcggcagtg
     1261 gcccggcagc ctgtttggct atttctttcg gttcaatgcc gatcgggtag acttctgttc
     1321 gaaatgcttt gccccaggct gtatggcttt ttgcgctacg tgtcgtgacg cgggtcaggt
     1381 tagaaagaca atccaggaac gccagacgat cgttttctgt ctggaaaccc agcaaatcat
     1441 aatcacaaag ctgttcaagc aaggtgtcat atgtcggcag cgcgttgaag atttccggtg
     1501 tcgggaaagg aatatgcaga aagaaaccaa tgcgattatt cactccccgt ttgcgtaatt
     1561 catgcgcaaa tggcaacagg tgataatcgt ggatccagat aatgtcatcg tcttgcaaca
     1621 gcggcagtaa tttatctgcc agcaacgcat ttacgcgtag atagccgtcc caggcaggac
     1681 gctgaaattg caccagatcg agccgataat gaaaagcggg ccagagaacg gcattggaga
     1741 attggttgta gtattcgtca aggtcctgtt cgctgaggtt aaaagaggcc cacgtaatgt
     1801 tacctttttt cacctttttt agcggctgat cctcattccc tgtttcacca ctccagccaa
     1861 accacagtcc gcctgcggct ttcagtgccc ccagtatgcc aacggcaagg ccaccggcac
     1921 tggcggcgtg ctcgtctggt ggtgcaatcc ggttagatac tacgactaaa cgactcatag
     1981 tcatcactcc tgttattttc tcttttttgt tgtaatgcgg tggttatcat ttcaagccag
     2041 ctccagacat ccggcacacc cgccagtcgc catgatgcct gagttgcacc tgtgccaatt
     2101 tttactgaca ttccgcccag tcggttaacg actgcgaagc cagattcatc ggttaaatca
     2161 tcgcccagaa atacgggcgt tcgcccgata aagggagctt cctgcataaa agctgcaatt
     2221 gcctcacctt tactggtacc tctcggtttg atctcgacaa cacactttcc ctgctgtaac
     2281 gccatttgtg gccagatctg agtaatacgt tgcgctaatg tcattaatgc gtcttcatgc
     2341 tgcggagcct gacgataatg cagcgcaaaa gccatccctt tcgcctccag ctccgcgccg
     2401 ggatactgag cgatgactgt atgcagttgc acgctaatat cacgcgcaat cgcatccggc
     2461 agatgaacga tatgtgtttt accattgatg tcacggcgct ccgccccatg cacgcccgct
     2521 aacgggaagc gataaggttt tgccagtgcg tcaagctcca ccattgagcg ccctgatatc
     2581 aatgccaatg caccatcact tgcggttgcc agtagctgta gtccttgcag aatattgtca
     2641 ggcacgacga cctgatcggg atgcggtttg atttccgcca gcgttccatc aagatcaaaa
     2701 aaccaggcat atttcgcgga tagttcaggg gtttcggtta acggttctgt cacccggctc
     2761 tcctccttct cttttcttat tgttttgcgc agaacgcgct ttacagacat gtaagtatag
     2821 acagtgtgac gggggtcgcc atttgcaatg aagaacagcc aaccgttgtg gcggctggct
     2881 gtggtgggaa aaaacgttaa attgttgtgg aaaaaagcat cagacaatgc gtttcgcttt
     2941 ttgcttgtaa cggtcgaaga tcaccgctgc cagcaggatt aagccgcgaa ccacgtactg
     3001 cgcgaaagga gaaatattaa gcaggttcat ggcgttttcc acggtgccta aaattaagat
     3061 acccgccacc acatatgaga tttttccgat gccacctttc agagaaacgc cacctaaaac
     3121 gcaggcggag ataacaataa gctcataacc aatcgacgtc attggctgcc cactagtcat
     3181 acgtgaagcc agaataattc cggctatcgc agataccagg ccagagagaa caaagataat
     3241 aattttggtg cgaacaaccg gtacacccgc cagacgcgca gcctcttcgt tcccgccaat
     3301 cgccagggtg ttacga
//
LOCUS       DAZSBF010000333         3296 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZSBF010000333 DAZSBF010000000
VERSION     DAZSBF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.338"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            149..667
                     /locus_tag="ACHXWD_004161"
     CDS             149..667
                     /locus_tag="ACHXWD_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR7622884.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            877..3018
                     /locus_tag="ACHXWD_004162"
     CDS             877..3018
                     /locus_tag="ACHXWD_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFR7622885.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3094..>3296
                     /locus_tag="ACHXWD_004163"
     CDS             3094..>3296
                     /locus_tag="ACHXWD_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622886.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALP"
BASE COUNT          809 a          830 c          969 g          688 t
ORIGIN      
        1 ttacgacaac cgtaaattct agctcggttt gtatgtaaaa aatactgaaa aatgatgcgg
       61 ttccgtgaag tgagctgcat ctgcctgtct gggattaacg gcaggtcatt gtaagtagta
      121 attaaccacg ttaacaagga gtttacaaat gccaacaccg tgttatatct ccatcactgg
      181 tcagacccag ggaaatatta ctgcaggtgc atttaccgct gattctgtcg gcaacatcta
      241 tgtgcagggg cacgaagacg agatgctggt gcaggaattc ctgcataacg ttactgtccc
      301 gaccactcct cagtccggtc agcctgcggg tcagcgtgct cacaagcctt tcatttttac
      361 cgtggcgctg aacaaagcgg ttccgctgat gtacaacgca ctggcctccg gcgaaatgct
      421 gccaaccact gaacttcact ggtggcgtac ctctgtggag ggcaaacagg agcattattt
      481 cactacccgc ctgactgatt cgacgattgt cgatatgaaa ctgcatatgc cgcattgcca
      541 ggacccggcg aaacgcgagt tcacccagtt gctcgaggtt tcactggcct accgcaaaat
      601 tgaatgggaa cacgtcaaat ctggcacctc cggtgccgac gactggcgcg caccactgga
      661 agcataagtt aagccaacag catccggcta tcaggccgga tgctgttttt gtattacgcc
      721 gtagaaagaa aggttgtcag taaggggtta cgttcacggg agatggcgga tgccggaaac
      781 ttcggcggta gcgtgcctga acgcaagcgt aacactgtac tgtcacagcc gctttaacag
      841 cgaactgtct gtttttcctt aaccccggag gttgctatgt caaccggatt acgtttcacg
      901 ctggaagtgg acggcctgcc accggatgct tttgcggtgg tctcctttca tctgaaccag
      961 tcactctctt cgctcttttc cctcgagctc tctctggtca gccagcagtt tctctccctt
     1021 gaatttccgc aggtactgga caaaatggcc tacctgacga tatggcaggg cgatgacgta
     1081 cagcgccggg tgaaaggtgt ggtgacctgg tttgaactgg gggagaacga caaaaaccag
     1141 atgctgtaca gcatgaaggt atgcccaccg ctgtggcgca cagggctgcg ccagaacttc
     1201 cgtatcttcc agaatgagga catcgaaagc atcctcgcga cgatcctgaa ggaaaacggt
     1261 gtgaccgagt ggagtccgct gttcagcgag ccgcatcctt cccgtgagtt ttgtgtccag
     1321 tacggcgaaa ctgattacga tttcctgtgc cggatggcgg cggaggaagg catcttcttt
     1381 tatgaggagc acgcgcaaaa aagtaccgac cagagcctgg tcctgtgcga caccgtgcgt
     1441 tatctgccgg agtcctttga gatcccctgg aacccgaaca cccgtaccga ggtaagtacc
     1501 ttctgcatca gccagttccg ctacagcgca caaatccgtc cttcttccgt ggtgaccaaa
     1561 gactacacct ttaaacgacc cggctgggca gggcgttttg atcaggaagg ccagtaccag
     1621 gattaccagc gcacacagta tgaagtgtat gactaccccg gacgtttcaa gggtgctcac
     1681 gggcagaact ttgcccgctg gcagatggat ggctggcgca acaacgcaga agtggcgcgc
     1741 ggaacaagcc gttctccgga gatatggccg ggacggcgaa ttgtgctgac ggggcatccg
     1801 caggcgaacc tgaaccggga atggcaggtg gtggcaagtg aactgcacgg cgagcagccg
     1861 caggcggtgc cgggacgcag tggttcagga accacgctga ataaccactt tgcggtaata
     1921 ccggcagacc ggacatggcg gccacagccg ttgctgaaac cgctggtgga tggcccgcag
     1981 agcgccgtcg tgacgggacc ggcaggcgag gaaatcttct gtgatgaaca tggccgcgtg
     2041 cgggtgaaat ttaactggga ccgctataac ccgtcaaacc aggacagttc atgctggatc
     2101 cgtgtggcac aggcgtgggc aggcaccgga ttcggtaacc ttgcgatacc gcgcgtgggt
     2161 caggaggtca ttgtggactt cctcaacggc gatccggacc agccgattat tatggggcgt
     2221 acctaccacc aggaaaaccg cacacccggt agcctgccgg ggacgaagac gcagatgacc
     2281 attcgttcga aaacctataa gggcagcggg tttaatgaac tgaagtttga cgatgcgaca
     2341 gggaaagaac aggtctacat ccacgtgcag aagaacatga acaccgaggt gctgaataac
     2401 cgcaccacgg atgtgataaa caaccatgcc gaaaaaatag gtaacaacca ggcgatcacc
     2461 gttaccaata accagatcca gaacattggc gttaatcaga tacagacggt tggtgtcaac
     2521 ctggtggaaa cggtgggcag taaccagatt atcaaagtgg gatcaaacca ggttgaaaag
     2581 gtggggatca ttcgtgcgct gacggtgggt gtggcttacc agacgacggt aggcggcatt
     2641 atgaatacct cggtggcgtt gttgcagtcc tcacaggtag ggctgcataa atcactgatg
     2701 gtgggaatgg gctacagcgt caatgtaggg aataacgtca ccttctcggt gggcaagacg
     2761 atgaaggaaa acaccggaca aacagcagtt tattctgccg gtgagcatct tgaactctgc
     2821 tgtggtaagg caaggctggt gctgacgaag gacggaagca tatttctcaa cggtacgcac
     2881 attcatctgg aaggggagtc ggatgtgaac ggtgatgcgc cagtgattaa ctggaactgt
     2941 ggtgccacac aacctgtacc ggatgcgcct gtgccgaaag atttaccccc cggaatgccg
     3001 gatatgcggc aattttgagt caggtgcaaa aaattaaaac ggaactggtg cagtgaccag
     3061 atgaaccata ttaaaagcag aaggaattga ttcatgggcg gcggaaaacc ggcggcgcgg
     3121 cagggcgaca tgacccgcaa gggactggat attgtgcagg gttcagcggg tgtgctgata
     3181 ggtgcgccga cgggagtggc ctgttcggtg tgtccgggag ggattaccta tgctaacccg
     3241 gtgaacccgg tgctgggtgc gaaggtgctt ccgggcgaga cggaccttgc gctgcc
//
LOCUS       DAZSBF010000334         3285 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZSBF010000334 DAZSBF010000000
VERSION     DAZSBF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.339"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHXWD_004164"
     CDS             complement(<1..229)
                     /locus_tag="ACHXWD_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622887.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKK"
     gene            403..1116
                     /locus_tag="ACHXWD_004165"
     CDS             403..1116
                     /locus_tag="ACHXWD_004165"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR7622888.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            1148..1906
                     /locus_tag="ACHXWD_004166"
     CDS             1148..1906
                     /locus_tag="ACHXWD_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622889.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1952..>3285
                     /locus_tag="ACHXWD_004167"
     CDS             1952..>3285
                     /locus_tag="ACHXWD_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR7622890.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          750 a          790 c          854 g          891 t
ORIGIN      
        1 atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat tttcccatca
       61 acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa tgaactcttt
      121 cggcagcacc atatttttcg acaacggtaa taagatgttc acacaccgtt gacttgcctg
      181 aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg aatatatcct
      241 ttaatttaaa catccattaa aaatatttat ttggttaata tgtttttatg aaagcgtaat
      301 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
      361 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      421 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      481 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      541 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      601 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      661 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      721 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      781 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      841 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      901 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      961 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
     1021 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
     1081 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
     1141 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
     1201 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
     1261 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1321 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1381 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1441 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1501 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1561 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1621 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1681 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1741 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1801 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1861 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1921 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1981 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     2041 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     2101 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     2161 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     2221 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     2281 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2341 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2401 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2461 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2521 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2581 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2641 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2701 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2761 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2821 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2881 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2941 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     3001 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     3061 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     3121 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     3181 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     3241 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAZSBF010000335         3268 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZSBF010000335 DAZSBF010000000
VERSION     DAZSBF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.340"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHXWD_004168"
     CDS             <1..493
                     /locus_tag="ACHXWD_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR7622891.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHXWD_004169"
     CDS             493..1083
                     /locus_tag="ACHXWD_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR7622892.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1832..2110
                     /locus_tag="ACHXWD_004170"
                     /pseudo
     CDS             1832..2110
                     /locus_tag="ACHXWD_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2170..2658
                     /locus_tag="ACHXWD_004171"
     CDS             2170..2658
                     /locus_tag="ACHXWD_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7622893.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2682..>3268
                     /locus_tag="ACHXWD_004172"
     CDS             2682..>3268
                     /locus_tag="ACHXWD_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR7622894.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSDIY"
BASE COUNT          853 a          730 c          943 g          742 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gattcaaaca
     1741 gaatggtgat ggcctgctgg atgtttatgc caatagcgtg catgtgttgc gttttcagag
     1801 tggcagtatc cagagtaata aagctgtaaa cgttacagga cgggtaacac cgtcagacta
     1861 cggaaacttt gatgcccgtt accagcagcg aaatggtggc gtgcaggatg tgcggtatgg
     1921 ttatgaaatg tattacaccc ccggcagtaa caccgtttcg tggacatttc gctcaccttc
     1981 gggacacggg ctgtcaggga tatcgatatc ggataccggc cgtaactcag cggataacgt
     2041 caacggcgtg tattaccgac cgctgcaaaa actgattaat ggcacctggt ataacgtagc
     2101 gagtatttaa caatgttgca tttaaaaaat attacggcag gtaatccgaa aaccgcggaa
     2161 taatatcagc tgacaaaaca gtatgatgtc acctggcttt tttcggaaga cggcaaaaac
     2221 tggtatgaag aacagaagaa ctttgccagt gacaccataa aaatggttta caccggagac
     2281 gggcgcgtgg tgtgggtcgg taaggatgtg acaggcattg aaccccgtaa cgccagtgtt
     2341 attgaagttc ctgatattac cgccaaccgc cgcattaccg cgccgggtta ctggttttac
     2401 cgcaatgatg aatttgtctt tgactacaga ctcaaagcgg aagatgagcg tgatgccctt
     2461 ctggctcagg tcagtgcccg gacaggggaa tgggaagaag acctgctgct ggggctaatc
     2521 agcgacgaag accgggaaaa actgaaagcg taccgtattt acgcgaaatc gctgcaggcg
     2581 atggatttca gcaccatcac tgataaatcc tcatataacg ccattgaatg gtccgtctct
     2641 ccggaaggtt cttcctgatt taatttatcg cgagaaaaac tatgtctgta gtgatatcag
     2701 gtgcgctgac tgatggcgca ggcatcccca tgtccggatg ccagattatt ctgaaatccc
     2761 gtgtaaacac ctcagaagtg gtgatgcgta ccagggctga tgtagtgacc ggaaataacg
     2821 gtgaatattc gtttgaggca caggtcggaa aatattgcgt gtatctgaaa cgggactggc
     2881 gcgacgagta ctgtgttggc gacattgctg tatacgacga ctcaaagccc ggcacgctga
     2941 atgactttct gattgctcct gatgagggcg acctgaaacc ggatgtcgtc aaacgctttg
     3001 aggaaatggt ggcgcaggcg cagcagagcg ccggggccgc agccggaaac gcacagcaga
     3061 cggcgcagga tgtggcggca gccgcaggtt atgcccgcgc agcagagcag gccaaaaatg
     3121 acattgatgc tgcgctgacc ggcactctga aaacggctaa ccatctgtct gaaatcgcag
     3181 cagcaggcga aaaggcacaa cagaagtccc gggataatct ggggctgaaa agtgcggcca
     3241 cgatggaagc acagagcgac atttacga
//
LOCUS       DAZSBF010000336         3222 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZSBF010000336 DAZSBF010000000
VERSION     DAZSBF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.341"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..263)
                     /locus_tag="ACHXWD_004173"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="ACHXWD_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHXWD_004174"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="ACHXWD_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFR7622895.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1175..1645)
                     /locus_tag="ACHXWD_004175"
                     /pseudo
     CDS             complement(1175..1645)
                     /locus_tag="ACHXWD_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1981..2166
                     /locus_tag="ACHXWD_004176"
                     /pseudo
     CDS             1981..2166
                     /locus_tag="ACHXWD_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2266..2472)
                     /locus_tag="ACHXWD_004177"
                     /pseudo
     CDS             complement(2266..2472)
                     /locus_tag="ACHXWD_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2525..2776)
                     /locus_tag="ACHXWD_004178"
     CDS             complement(2525..2776)
                     /locus_tag="ACHXWD_004178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622896.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGSFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          909 a          805 c          657 g          851 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt accaccaacg gcctaatata tacagaacgg agtaaacagg gctaacagtt
     1201 cccgacaggt ctcatcattc cgtggcccga aagtgtaagc caacacaccg ccagttttag
     1261 tgttgtacgc ataccagagc cagtgttgtt gcagccttgc tgccaacgaa gcgccactgc
     1321 tcatcaagtt cacagataag ggcaacatca gcgtgagcga ctggcgcaga tgttatccgc
     1381 cggggcggga gctttttaat gtccgtatga cggtattgat gccgattttc agtgtcctgg
     1441 cggtatcacg gccccctgcg ccgttatgtg ccatttcaac gatctgctcc ttaacgccgg
     1501 gttttctggc ttcataagag taagtgagct ggaaaacacg tttacatgag cgacaacgga
     1561 aacgctcatg ccgggaacaa ctacggccat gtcgataaac ttcatcggaa tgacagcgcg
     1621 gacaatgaac agtaacggta gccatggaaa accttaaata ccagtagatt accagaaact
     1681 tctgtcgatt gaagacatca cccgaagttt gaattcattc gaatactttt tacgaggttc
     1741 ttttcgccag tcctgtaatt ccatacttag gtgttcgtct atgtcagatg ggcgtctaag
     1801 ttaccaattc ttgtctaatg gctacatacg gtggtcggtt tacgcttacc gcaggacagg
     1861 tggtaactga aaaatagttt gctgaggcga ctggaaacag aagggcaaga tcgattccga
     1921 cagcagtgat ggtcagctac ctcatcgata actttgcaac agtgcccttc ttgaagcatc
     1981 atgcttcatg aagaaaaact ggcacgtcat caacgtaaac aggcgatgta tacccgaatg
     2041 gtagccttcc cggcggtgaa aatgttcgaa gagtatgact tcacgttcgc caccggagca
     2101 ccgcagaagc agctccagtc gttacgctca ctcagcttca tagagcgcaa tgaaaacata
     2161 gtgttacagg ggacatctga catcacaaat ccccgggtgg ggatttgtgt ataagagaca
     2221 gcccatggag gaaggggata tatcgcagct ctgacgactc actccttatt tacccgggaa
     2281 atacttctcc agcaatgcat acaggatggc agcggtttca gcatccatta tgccgtcata
     2341 atttttctgc cggaaatgaa gctgaaaagc gcggacaagc gccttatatc cgtcatccgt
     2401 ctccacacca gaaatactgt atccataggt cttcagccgt gacacgatat caaccttatc
     2461 cggaatgccc tgcgattcaa aaaataaata tttagtcagt aattttatgt ttttcctgaa
     2521 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
     2581 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
     2641 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gagcctgaca
     2701 gccctaacag aataagttta cgcgtaaatc gcccggaatc cagaagagca ttaagtatca
     2761 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
     2821 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
     2881 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
     2941 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
     3001 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
     3061 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
     3121 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
     3181 tctgaaagta atcactatga ttaaatatga ttaacaacta at
//
LOCUS       DAZSBF010000337         3206 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZSBF010000337 DAZSBF010000000
VERSION     DAZSBF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.342"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACHXWD_004179"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="ACHXWD_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR7622897.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACHXWD_004180"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="ACHXWD_004180"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR7622898.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="ACHXWD_004181"
     CDS             1223..1492
                     /locus_tag="ACHXWD_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR7622899.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="ACHXWD_004182"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="ACHXWD_004182"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR7622900.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="ACHXWD_004183"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="ACHXWD_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR7622901.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          773 c          778 g          835 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgaca ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtttttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatggccgc
      661 cagccagacg cataaatgcc attacccggc gttctcctgc ttgcagatat tctagtaata
      721 aaggctcaat agcacggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagccg ggaagctaaa acatggggaa
      841 tgtaaggcgt atcgcacgga caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tggcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgttg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcaaggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag agcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gacggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcaca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttggcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgtcc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt caaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAZSBF010000338         3141 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZSBF010000338 DAZSBF010000000
VERSION     DAZSBF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.343"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..693
                     /locus_tag="ACHXWD_004184"
     CDS             <1..693
                     /locus_tag="ACHXWD_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain-length determining protein"
                     /protein_id="HFR7622902.1"
                     /translation="NLSTEQDKKIWLAEQANKGLVITPPKEKGNTSYYIIQASADSAQ
                     EAYKLLQGYLKNVNNQAVTLSLDEFGQNVNTLLVNLNKEIIDIDFQRKSEKLDQIAHI
                     QRDLTTAEQAGIIDYRSSKGGFDNAQSSYKFLLGEKLLSAELKATKDAPIIYPFRYYE
                     VKRQIDELEGMLRDNIQAQAYRYQMKPSEPVIKDKPNKALILILGALPGAMFAIVGTL
                     VYATLKDKTKLD"
     gene            762..2042
                     /gene="tviB"
                     /locus_tag="ACHXWD_004185"
     CDS             762..2042
                     /gene="tviB"
                     /locus_tag="ACHXWD_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vi polysaccharide biosynthesis
                     UDP-N-acetylglucosamine C-6 dehydrogenase TviB"
                     /protein_id="HFR7622903.1"
                     /translation="MKFDTLNAKIGIIGLGYVGLPLAVEFGKKVTTIGFDINKSRIDE
                     LRNGHDSTLECSNLELLEATKLTYACSLDALKECNVFIVTVPTPIDKHKQPDLTPLIK
                     ASETLGKIIKKGDVIIYESTVYPGATEEDCIPVVEKVSGLKFNIDFFAGYSPERINPG
                     DKEHRVTNILKVTSGSTPDVAEYVDQLYKLIITVGTHKASSIKVAEAAKVIENTQRDV
                     NIALINELSIIFNKLGIDTLEVLEAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKAQS
                     VGYHPEMILAGRRLNDSMGQYVVSQLVKKMLKQRIQVEGANVLVMGLTFKENCPDLRN
                     TKVIDIISELKEYNINIDIIDPWCSTDEAQHEYGLTLCEDPKVNHYDAIIIAVAHNEF
                     REMGESAIRALGKDEHVLFDLKYVLDKKSIDMRL"
     gene            2135..>3141
                     /locus_tag="ACHXWD_004186"
     CDS             2135..>3141
                     /locus_tag="ACHXWD_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR7622904.1"
                     /translation="MSRYEEITQQLIFSPKTWLITGVAGFIGSNLLEKLLKLNQVVIG
                     LDNFSTGHQYNLDEVKTLVSTEQWSRFCFIEGDIRDLTTCEQVMKGVDHVLHQAALGS
                     VPRSIVDPITTNATNITGFLNILHAAKNAQVQSFTYAASSSTYGDHPALPKVEENIGN
                     PLSPYAVTKYVNEIYAQVYARTYGFKTIGLRYFNVFGRRQDPNGAYAAVIPKWTAAML
                     KGDDVYINGDGETSRDFCYIDNVIQMNILSALAKDSAKDNIYNVAVGDRTTLNELSGY
                     IYDELNLIHHIDKLSIKYREFRSGDVRHSQADVTKAIDLLKYRPNIKIREGLRLSMPW
                     YV"
BASE COUNT         1060 a          464 c          645 g          972 t
ORIGIN      
        1 aatttatcta ctgaacaaga taagaaaatt tggttagctg agcaagcgaa taaaggtctt
       61 gtgattacgc caccaaagga aaagggaaat acaagttact acataataca agcatcggca
      121 gactcagcgc aagaggcata taaactactg cagggatatc taaagaatgt taataatcaa
      181 gctgtaacat taagtcttga tgagtttggt caaaatgtta atactctttt ggttaatcta
      241 aataaagaaa ttattgacat agatttccag agaaaatcag aaaagcttga tcaaatagct
      301 catattcagc gagatttaac aactgcggaa caagccggaa tcattgatta tcgctctagc
      361 aaaggcggct tcgataatgc gcaaagtagc tataagttct tgctcggcga aaaactgtta
      421 tcagcagagc taaaagcaac taaagatgcg ccaattattt acccatttag atattacgaa
      481 gtgaaacgtc aaattgatga gttagaagga atgttacgcg ataacattca ggcgcaagca
      541 tatcgatatc aaatgaagcc atctgagcca gttataaaag acaaacccaa caaagcatta
      601 attttgattc ttggtgcatt accaggggca atgtttgcta tagttggtac attagtttat
      661 gcgacattaa aagataaaac caagttagat taaactgggt tacgtattgt tgtgtcaatg
      721 cgaaatagat gttctatgtg cactttatga tggataagaa aatgaaattc gatactttga
      781 atgcgaaaat tgggattata ggccttggtt atgttggatt gcctcttgct gttgagtttg
      841 gaaagaaagt aacgacgatt ggatttgata ttaataagtc tcgtattgat gaattacgaa
      901 atggtcacga tagtacatta gagtgctcaa atttagagtt gttagaagca actaaattga
      961 cgtacgcctg ttcattagat gcactaaaag agtgtaatgt atttattgta actgttccaa
     1021 ctccaattga taaacataaa cagccagatc taacacctct aattaaagca tctgaaacat
     1081 tgggtaagat aataaagaaa ggcgatgtta ttatttatga gtcaacagtt taccctggag
     1141 cgacagaaga agattgtata ccagttgtag agaaagtatc aggtcttaag tttaatattg
     1201 atttttttgc cggttattca cctgagcgta ttaatcctgg ggataaagag catcgtgtaa
     1261 ctaatatcct taaggtgacc agtggatcta caccggatgt tgctgagtat gtagatcagc
     1321 tatataaatt aataattact gtcggtacgc ataaagcatc atcgataaaa gtagcagagg
     1381 ctgcaaaagt aattgaaaac acgcagcgag atgtcaatat tgcattgatt aatgagttat
     1441 ctattatatt taataagtta gggattgata ccttagaggt tcttgaggct gcaggtacga
     1501 agtggaattt tttacctttt aggcccggtt tagtaggtgg ccactgtata ggtgtagatc
     1561 cttattatct tacacataaa gcgcaaagtg tcggctatca tccggagatg attttagccg
     1621 gacgtcgttt aaatgatagt atggggcagt atgtcgtttc ccagttagtc aaaaaaatgt
     1681 tgaaacaacg gattcaagtt gaaggggcga atgtgttagt gatggggctt acatttaaag
     1741 agaattgccc agatctacga aacactaaag tgattgatat tatttcagag ttaaaagaat
     1801 acaatatcaa tatagatatt atagatccat ggtgttctac cgatgaggca caacatgaat
     1861 atggattaac tttatgtgaa gatcctaaag ttaatcatta tgatgcaata attatcgctg
     1921 ttgcacacaa tgagtttcgc gagatgggag agagcgctat tcgtgcatta ggtaaagacg
     1981 agcacgtttt gttcgattta aaatatgtgc ttgataaaaa aagtatcgat atgcgcttgt
     2041 aagagtgatt aaaaaaatca aatcctcttt gatatgatac acctcagcat tttatgctag
     2101 gtttagcact tgattaatat acatggatat ttatatgtct cgctatgaag agattacaca
     2161 gcagttaatt ttttcaccga aaacttggtt aattactggt gtcgctggct ttataggatc
     2221 aaatctttta gaaaagttac ttaaattaaa ccaggttgtt attgggttag ataacttttc
     2281 cacgggacat caatataatc ttgatgaagt taaaacatta gtttccactg aacagtggag
     2341 tcgattttgc tttatagaag gtgatattcg agatctcact acctgtgagc aagttatgaa
     2401 aggtgttgat catgtcttac atcaggctgc gctaggttct gtacctcgtt caattgttga
     2461 tcctataaca accaatgcaa ctaatattac tggatttttg aatatcttac atgcggctaa
     2521 aaatgcacaa gtacaaagtt ttacttatgc tgcatcaagc tcaacttatg gagatcatcc
     2581 cgcactacca aaagtagagg aaaacattgg taatccactt tctccttatg cagttactaa
     2641 atatgttaac gagatttatg ctcaggtata tgctcgaaca tatggtttta aaactattgg
     2701 attacgttat tttaatgtat ttggtcgtcg tcaagatcct aatggagctt atgctgcagt
     2761 aattccaaaa tggacagcag caatgcttaa aggtgatgac gtatatatta atggcgatgg
     2821 tgaaacgagt cgtgattttt gttatataga taatgttata caaatgaata tattatctgc
     2881 attagcgaag gacagtgcta aagataatat atataatgtt gcagttggtg atagaacaac
     2941 gttaaatgaa ttatctggtt acatttatga tgagcttaat ttaattcacc atatcgataa
     3001 attgagcatt aagtatagag agtttagatc tggagatgtt aggcattctc aggctgatgt
     3061 tactaaggct atagatttac taaagtatag accaaatata aaaatcagag agggattacg
     3121 actttcaatg ccgtggtatg t
//
LOCUS       DAZSBF010000339         3112 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZSBF010000339 DAZSBF010000000
VERSION     DAZSBF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.344"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1258)
                     /locus_tag="ACHXWD_004187"
     CDS             complement(<1..1258)
                     /locus_tag="ACHXWD_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR7622905.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLIERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLLN"
     gene            complement(1415..1624)
                     /locus_tag="ACHXWD_004188"
     CDS             complement(1415..1624)
                     /locus_tag="ACHXWD_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622906.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1647..2357)
                     /locus_tag="ACHXWD_004189"
     CDS             complement(1647..2357)
                     /locus_tag="ACHXWD_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7622907.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2873..>3112)
                     /locus_tag="ACHXWD_004190"
     CDS             complement(2873..>3112)
                     /locus_tag="ACHXWD_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622908.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          913 a          653 c          507 g         1039 t
ORIGIN      
        1 aattaagtaa attagtcagt gaccttgtaa agtctgttaa accttcctgt gaaccatcga
       61 acattaaggc cagattatta ttccatgaat tacttaagcg gcctaactgc gtctgagtag
      121 agttaataat cttttcccat ccaccagctt tatcaatcat ccttcccatt tcagcggata
      181 ctttaggaaa aacatcttta gaaagaacct taccgtcttt catggccttg tctaattctt
      241 ccgtagtcat cccaagactt ctggcaaata actgaaaagc acctgccatt ctgtcaccta
      301 attgaccgcg tagctcttca gcctgaatct ttcctttaga tgccatctgc tcgaaagctt
      361 ttaaaatccc gttaatttca tcggtgctta gccccattaa tctggatgta ccaatcacat
      421 tagaaaataa ggattctttc tggtctgcat tcatatcacc agcaccagcg gaaaacttag
      481 caaaactgct taatgtttca atctggtcta caccactact atcagcaaga ttcttaacaa
      541 attctgtagc tcgtttagca ccttcatcac ctaaaacgaa ttgtgccgct cgttgtgatg
      601 cttctttttt cagaccaacc tccataatcc gcctgtaagc ttctaaggct gtctgaaaac
      661 tataaatggc tgtacctgct ttgattgcac tcgtagctaa tacatccaga ctatttgaag
      721 cattttcact gtgattattt ttgctggtgg tcttgttaaa cttagaaagt gtttcacgaa
      781 gagactgatt taactttgct aactcacgat taaaagtttc gtttccaagt gtcttggatt
      841 tactgcttaa gctgtaatac tggcttttaa aatacttctc aagcatagga ttaaagtcac
      901 gaagcttgct catattcgct gagtaagctg ctgtgattgc tctgcttccg ctaattcgtg
      961 gctgtcttgg ttgtctaagt ggctttaaag tatcctcaat ttcagtttta atcttaaacg
     1021 ctctagtgtt tttaagacgc tgctcaatct tggaaacagt tttatcgaca tttctttcta
     1081 ttagtttgag agaatcattt aaaggcgtag gattaactct aaacgactta ttcaatcgct
     1141 tctcaattcg tgatgctacc tgcatcgttt gtttctcggc tttttccaag ccttttaaaa
     1201 ctttctgaga gttaaaactt aactcaatca gaaaatcatc aactttaaat tgtgccattc
     1261 atatttttca cctctttaag cgtgttgtac gtaaattcta tttttcagtg tgggtattag
     1321 ctttacagat ttcacgtagc acacatacaa aaaaagggag agctaccgat gtcactcgat
     1381 aatctctccc cgtatttacc caaccaattc ttttttaatg tcggacaaga taatgtaatt
     1441 ccgtttcacc tatgatttga gcatcagaag gaatacggtc aatacaccac tttggaatct
     1501 tgcgtgtttt tggtttgtgt gaggtgataa cttgacgttt gtttttaatt cttaaggctg
     1561 gcttagtttg taactttaag ttttcacaaa cagaccgaat agtctctaaa cgttcttcaa
     1621 acattaatca cctctttaat tcataatcaa acagccttgc tatacttctg agaaatcttc
     1681 gttaactgct caatatcagc catcaaagca atcttcttct gcgactcata ttcactaaca
     1741 agttctttca tagtatcaag ttctttttta agcttttgct ttaacttatc cagtttaccg
     1801 gaataatgct tagtcaaatt agcttcagct aactgaagaa tctcttcatc tgttttatca
     1861 gtcatcagaa taaattgacc agaacccctt aatgcttgtc tgacatcaat taaccggaac
     1921 ccttcaggga tactttgaag aactttagga acgtcttcaa tcgctgtacc aaaaccatca
     1981 ttaaagtttc cttcataata gataaacggg tttgacttaa tctcgtaagc aatatcatat
     2041 tctgaaggtt tcgaggtttc ttcgaagttc ttaacagcat cgtaagcgac atcaagtcta
     2101 agacgctgtg attttaaagc tgggtaatat tcacgctcaa gctcaaatag tgcttcagtt
     2161 ttgcagatat caggcatttc ttgaggctca ggagacacaa aggaaattac ctttggtgac
     2221 atctttagct cttgctcaag ctcaatgcgc ttgtcatgct cttgacgctg aatagtatgt
     2281 agcggatgtt caccctgtgg tgaccagtct ccgaagctat caaccttttt accgttaata
     2341 tataaatcgt ttgtcattat aatttcctct aagtgtggtt atttcttaat agagcacctt
     2401 caacgccaat acacttagaa gatgacttaa cgatttatta aagatgctct attaagaaaa
     2461 gccctaaaca taatctgtga agggctttgg aggtttgctt ataacgttaa cgaagtttca
     2521 ggaaaacttt ctttaaaagc tctgtttaaa aactcttaaa gaaagcccta actcatgatt
     2581 agggcaaagc tgtaaaggat tcaataatga atgaacaaag actttttgag atagcctctc
     2641 agaccaacct gaaaggctag gacgaaaagc attgcacctt tccaactcag aatatccatt
     2701 catcggatta ttgaaagaaa aaataagaga gaattgctct cccccatata tacaattatg
     2761 tcatgctcaa ggttgtttgt caacagcctt taacagattg gtaacattta tactttaata
     2821 gatgcacctg caatcatacc aatcaataca aaacagataa tcaccaattc cattaatgca
     2881 ccaccttcga atccgtcacc agaatattat cgctatacaa aattgctata gccgccttga
     2941 gatatttttc agggctaacg ctgtatttgt tcgtttcttc gataataaga cgcttcatgt
     3001 ttgagtcggt aacaggaaac ataaaacgcg gttcaccagt ttcaatttcc tgaataaagt
     3061 cttcaatttc ttctgagttt tcagccatta gctttagtaa tttatcaagg tc
//
LOCUS       DAZSBF010000340         3095 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZSBF010000340 DAZSBF010000000
VERSION     DAZSBF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.345"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            392..1078
                     /locus_tag="ACHXWD_004191"
                     /pseudo
     CDS             392..1078
                     /locus_tag="ACHXWD_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1411..2286
                     /locus_tag="ACHXWD_004192"
     CDS             1411..2286
                     /locus_tag="ACHXWD_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR7622909.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2294..3070
                     /locus_tag="ACHXWD_004193"
     CDS             2294..3070
                     /locus_tag="ACHXWD_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR7622910.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          840 a          729 c          756 g          770 t
ORIGIN      
        1 aaaaactggt cgcagaaaag tcctgttttc agcttggaaa cccggtaatc agaataatgg
       61 ataggggaac aaaaatctct gtatagaacg ctccattgag ggagagaaaa cgtgtcattt
      121 tgacccgttc ggtattcaga acacacaaaa ccgatcgcgg gtcgctttcg tgcatagtta
      181 tgcaacgcgc taaaaacgat tctgacgcat tttttatgtt taccctgttt tttcttgtct
      241 ttttgagttt tttgcgtcag agagcgtctg agagggcacg tacaacggga gttatggtaa
      301 atggatcggg ttttcgggaa ggttcgacag gatttgccgt tgggtgtagt gtaagcgact
      361 gaaaaacaaa cgcccctgaa atcatgtcca gatccggcag aaccagcctc atgcgcagtt
      421 ctggctggtt tttgacgttg atcgcgaggg ggcagcgatt gactggagcg accggaacgc
      481 tcccgcgccg aacatcaccg taaaaaatcc tgtgaacggt catgctcacc tgctctacgc
      541 gctcaatatc gccgtgagaa ccgcgccgga cagctcggtt aaggcgctga aatacgccgc
      601 tgcgattgaa cgttcgctgt gtgagaaact ttgtgctgat gtgaactaca gcggactgat
      661 ttgtaaaaat ccgttccacc tggaatggca ggtgatggag tggcgagagg aagcctatac
      721 cctcgatgaa ctggctgatt atctcgattt gagcgcctca gcgcgccgta gcatcgataa
      781 acattacggg atggggcgaa actgccacct gttcgaaatg acgcgcaaat gggcttacag
      841 ggcgattcgt cagggctggc ccgaattctc acagtggctt gatgccgtga ttcagcgtgt
      901 cgaaatgtac aacgcatcgc ttcccgttcc gctttcaccg cctgaatgtc gggctattgg
      961 caagagcatt gcgaaataca cgcacaggaa cttcacgccg gaaactttcg cacagtatgt
     1021 ggctgatacg cacacattaa cgtacgtttt cgttccattg gccctcaccc cccgataacg
     1081 acccaggtag cgcgccgtat tccccccgcc ggccgttttc ttcgacatgt acatcaggcg
     1141 cggaactcat cggggcgcgg gaatgaaatc ccattcagac tccggataga ttcaagcaag
     1201 cccacttatc gtcaaaatcg gtgttgcaaa aacgggagtg accatagatc cgttttccaa
     1261 gcggacccca taatatcggc tggtaaacat cactcgctag tgcattcttt ttgttggtaa
     1321 tgataatcat ttacattcat tctttgatgt tttatcataa ttaatgtttt gtcaataaat
     1381 atatggaaac atattgtttc agatgggaat atgagaaagt tcatccttat ttctatgata
     1441 acgttagcta gtgttagtct gggagcctgc agacagaatg tcaccataaa gcaggatgcg
     1501 ccagggcaga aagctttcct gactgacggg cagatttacg atttgcacag tggcaaaatt
     1561 atttctagca gcgagctttt ggcggatctt gctaccgctc gacatcttat tattggtgaa
     1621 aaacacgata atgccgagca tcatcagatt gagctttggc ttattcaaaa tttgctcatc
     1681 cagcgaccac aggggagtgt gttgcttgag atgttgacgt cagagcaaca gccccgagtt
     1741 aaccaagtta aatgttggct gaaagataat cctgtggttc gagatagccg tgttcaggaa
     1801 ctacttaact ggcaaaaagg gtggtcctgg gagatgtatg gcgacatcgt catgcagcta
     1861 ttacgtgggc cttacccgct gctcaatgcc aacattggcc gggagcagat gttggcactg
     1921 tacaaaaaaa acgaatttcc taaaggaaaa aaatccactg cacctgtggt acaagaagct
     1981 ctgcgtgaaa ccatcatatc tatgcatgaa ggcaaccttg aaagccagca actgacgtca
     2041 atgctctcta ttcagcagca gcgggatcgc tacatggccc gccagcttct gagtgccccg
     2101 gtaccgtcgt tgttgattgc aggtggttat cacgcgtcaa aaagcatggg cgtaccgtta
     2161 cacatggaag atcttgccac cggcacccat ccagtggtgt tgatgcttgc agaaaaagga
     2221 atgaacatca cagtagatca tgctgactac gtgtggtttg tagcgccaga tacaacaaaa
     2281 aggtaaacca tggatgatga atattctcca cttctcacag tcggtaaaat ggagcagctg
     2341 gtttatctgt tctctactgc ttcatggact gattttcctt gcgtttatct ggcgtttttc
     2401 cgaagttcag cccgctatgt ctcctgcacc ggcgatcatg ctgcaatggg cagaaattat
     2461 agaggctcct tcttctccac tttcgcttcc ggtgggaatc gctcaacagg agtccgcagt
     2521 gacagaagaa aagcaacaaa ctgaggacag acaacaacgt cctgtcactg aagacagtga
     2581 tgcaaccatt gaaataacta ggaagaaaaa gtcatccgat ggagaaaaga aaaagacacg
     2641 tccacctcgt aagataaagg ctcaaaccag cgacagcaac cccacagccg tttccagtaa
     2701 tgctgcacct caggctctgg tcgaatcgtc ccgtattgct gcgcctttta acagtgactc
     2761 aacaaagcga gataattctg aggcatcttg ggaaagcaga gtaaaaggcc acctaaaccg
     2821 ttataaacgt taccctggcg atgccagaaa acgtgcacga accggcacag ccgtcgtgac
     2881 ttttaccgta aacacggaag gcactattgt cagtagtttc ttggaaatct cttcaggaac
     2941 cctttctctt gaccgcgaag ctattgctgt tttagaacgc gctcaaccac ttcccaaacc
     3001 accgcctgaa atattggagg gaggattatt taaagtaaaa atgccgatta cctttaagtt
     3061 gaaggagtaa agggataatt ggccgttcaa gttca
//
LOCUS       DAZSBF010000341         3048 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZSBF010000341 DAZSBF010000000
VERSION     DAZSBF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.346"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHXWD_004194"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHXWD_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR7622911.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHXWD_004195"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHXWD_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR7622912.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
     gene            2111..3048
                     /locus_tag="ACHXWD_004196"
                     /pseudo
     CDS             2111..3048
                     /locus_tag="ACHXWD_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          864 a          689 c          843 g          652 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacggtc aaacatcgtg
     2101 gcgttgacag cacacgtagg gtgaagccgg gctgaagcgg gtggtgaaaa aggaacacgc
     2161 ggacggcagc gtcacgcaga gtcagtttga cgccgtgggc aggctcagga cacagacgga
     2221 tgccgcaggc cggacaacag aatacagccc ggatgtggtg acgggtctca tcacgcgcat
     2281 caccacgccg gatggcaggg catcggcgtt ttactataac caccacagcc agttaacgtc
     2341 agccaccggg cctgacgggc tggaaatacg ccgggaatat gatgaatggg gcaacctgct
     2401 gaatgaagag aacccgcatc agctgcagca gcttattcgt ttgccggggc agcagtatga
     2461 tgaggagtcc ggcctgtatt ataaccgcca ccgctattat gacccgctgc aggggcggta
     2521 tatcactcag gatccgattg ggctgaaggg gggatggaac ctgtatacat atccgctgag
     2581 cccggtgaat agcatggatc cattaggatt atatgaattt aaatcaaaaa atattgatga
     2641 tattggaata tttgcattgg caatgtgtaa tggagaatca attaacgaga ataaagaata
     2701 tggtggacta atatgtaaga agcaaggtga atatttcccc atgaatccga taagttcaaa
     2761 tgataatgat agtgtagact tgcgaaatat aaaatgccct gaaggttcag agagagtagg
     2821 cgattatcac actcacggtt tttactctga cgataaagga aataaagtaa caaaagaaaa
     2881 tgatgtttat gatagtctaa atttttcaag caaagattta acgaattctt atatgaatgg
     2941 aatgggaaaa aaagaataca gtagttactt gggaacacca aataacacct atctaaaata
     3001 taatcccaaa gctaaaggga atggagttac aattatcagg caagggag
//
LOCUS       DAZSBF010000342         3046 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZSBF010000342 DAZSBF010000000
VERSION     DAZSBF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.347"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            95..577
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004197"
                     /pseudo
     CDS             95..577
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            complement(800..1156)
                     /locus_tag="ACHXWD_004198"
     CDS             complement(800..1156)
                     /locus_tag="ACHXWD_004198"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HFR7622913.1"
                     /translation="MIQAQEYIGAGYHWVVDLDLEKFFDRINHDVLMSRIEKRVSDKR
                     VLSLIRRFLNAGVMDAGLVRPVTEGTPQGGVISPLLSNLFLHYAFDMWMQRQCPDVPF
                     ERYADDGVPRRRAPGT"
     gene            complement(1217..1420)
                     /locus_tag="ACHXWD_004199"
     CDS             complement(1217..1420)
                     /locus_tag="ACHXWD_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622914.1"
                     /translation="MRVSELPDYLRHHWPELKAQLLSGRYRPSPVRRVSILKPGGGER
                     LLGIQMVVDRFIQQAMMQVLQAL"
     gene            1446..2228
                     /locus_tag="ACHXWD_004200"
                     /pseudo
     CDS             1446..2228
                     /locus_tag="ACHXWD_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            2806..>3046
                     /locus_tag="ACHXWD_004201"
     CDS             2806..>3046
                     /locus_tag="ACHXWD_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFR7622915.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFD"
BASE COUNT          650 a          868 c          811 g          717 t
ORIGIN      
        1 cggcacggaa ccacggcagc ttgacggctg caatgacgtg tgtcgtctgg cggtggagta
       61 tctgctgtat gcagcccgaa aacgggggct ggaaatcggc atcttctgca ccatccacac
      121 tttacgcctt cactttgaag aacatttacc cctcgttgta gccggacgtc gcctgggcgt
      181 accaaaatca acagtttgta gtatgtttgt gcgcttccgc aaagctggcc tttcatggcc
      241 tctgcccgca ggtatgtcgg agcgggaact tgacgcccgt ctttacggga gtgcctccac
      301 agtacctgtc gtacttactg aaagtaccgt tatgcccgaa gtccccgggg taaaaaagcg
      361 tccccggcgc cccaacttcc cttatgagtt caaaatcgcc ttagtggagc agtcactgca
      421 gcccggagcc tgtgtggcgc agattgcccg tgaaaatgga atcaacgata acctgctctt
      481 caactggcgc catcaatacc ggaaaggtgg cctgctgcct tccggaaaaa atatgccggc
      541 actgcttccc gtgacgttaa cgccggagcc ggataaccat ggttttgaca tcatctatat
      601 gctttcgaca catcatcagc ggttcacttt cgttcgtctc ttcgatccat acctgatcgg
      661 ctcacgtccg accttttccc atctcgctca ccaccacatc tcttaaatgc agcagcagtg
      721 ggtggtttgg tgcctgcgcc tgcacgccga caccgagggg ccttccctca tctcttgtgc
      781 agcatggctg cacagggact cacgtccctg gtgcgcgcct tcgtggcaca ccatcatcgg
      841 cgtacctttc gaagggaacg tcgggacact gacgttgcat ccacatatca aaggcatagt
      901 gcaggaacag atttgagagc aggggcgaga tcacgccgcc ctgcggcgtt ccttctgtca
      961 ccggccttac cagacctgcg tccattaccc ctgcattcag gaacctgcgg ataagtgaca
     1021 acacccgttt atccgatact cgtttctcta tccggctcat cagcacgtcg tgatttatcc
     1081 ggtcgaagaa cttctccaga tcgaggtcga ccacccagtg atacccggcc ccgatatatt
     1141 cctgggcctg tatcactgcc tggtgggccg aacgaccggg acggaacccg tagctgttgt
     1201 cactgaacga gctatctcac agtgcctgca atacctgcat catagcctgc tggatgaagc
     1261 gatccaccac catctggatg cccagcagac gttcgccgcc gccgggcttc agtatggata
     1321 ctcttctcac aggggatggg cggtatctgc cggacagcag ttgcgctttc agctctggcc
     1381 agtggtgcct caggtagtcc ggcagttcac ttacgcgcat gccatcgact cccgccgcac
     1441 cttgattgcg tcttgtcgat ggaacagcaa tcagcgcgcc cgggggcggc agcgctgaat
     1501 ggcgactaca tatgggatat gatcctcata cctgtcagtt cactgatttt gagctaaccg
     1561 acagcagaga cgctgaacgg ctggatcgat ttgcgcaaac ggcagacgag atacgaattg
     1621 ctgaccgggg attcggttcg cgtcccgaat gtatccgctc acttgctttt ggagaagctg
     1681 attatatcgt ccgggtttac tggcgaggat tgcgctggtt aactgcagaa ggaatgcgct
     1741 ttgacatgat ggattttctg cgcgggctgg attgcggtaa gaacggtgaa accactgtaa
     1801 tgataggcaa ttcaggtaat aaaaaagccg gagctccctt tcctgcacgt ctcattgccg
     1861 tatcacttcc tcccgaaaaa gcattaatca gtaaaacccg actgctcagc gagaatcgtc
     1921 gaaaaggacg agtagttcag gcggaaacgc tggaagcagc gggccatgtg ctattgctaa
     1981 catcattatc ggaagatgaa tattcagcag agcaagtggc tgattgttac cgtctgcgat
     2041 ggcaaattga actggctttt aagcggctca aaagtttgct gcacttggat gctttgcgtg
     2101 caaaggaact tgaactcgcg aaagcgtgga tatttgctaa tctactcgcc gcatttttaa
     2161 ttgacgacat aatccagcca tcgctggatt ttccccccag aagtgccgga tccgaaaaga
     2221 agaactaacg cgttgtggag aataacaaaa atggtcatct ggagcttaca ggtggccatt
     2281 cgtgggacag tatccctgac agcctacaaa acgcaattga agaacgcgag gcatcgtctt
     2341 aacgaggcac cgaggcgtcg cgttcttcag atggttcaac ccttaagtta gcgcttatgg
     2401 ggtaaatccc cgcctttttc ctgccggtcc ccggacagag cacaaaggaa gtcgccgccg
     2461 gctccgcttt acccggccat gcggggcatg gccttgtggg ttttcagctc tgtagcctca
     2521 gcgtcgtgtg cgggctgtgc cgtgcctcca tcttagccgg gctggcgttg atgcaagggt
     2581 acgcttcgcc gctgcggtca cccggtccct ccttcccgtc tgccgtgatt ttccggtcgt
     2641 tcacccgctc agatttcgcg gtttcaccct ccaactcccg ccagccgtgt gcgtcaggct
     2701 gcggcttccc ttgcgccctg tgcatccccg ccttatcgcc cggctaagat ggaggcacgg
     2761 caccgcccgg tgtcgcggaa tatgtgaaca acggaggaaa acatcatgca atatgcgaaa
     2821 cctgtcactc tgaatgttga agagtgcgac cgtctctcct ttctgcctta cctgtttggc
     2881 aacgattttc tgtatgccga ggcgtatgtg tacgcgctgg cgcaaaaaat gatgccggaa
     2941 tatcagggcg gcttctggca cttcatccgc ctgccggacg gtggcggtta catgatgccg
     3001 gatggcgacc gtttccacat ggtgaacggt gcaaactggt ttgacc
//
LOCUS       DAZSBF010000343         3045 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZSBF010000343 DAZSBF010000000
VERSION     DAZSBF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.348"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHXWD_004202"
     CDS             <1..172
                     /locus_tag="ACHXWD_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622916.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHXWD_004203"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHXWD_004203"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR7622917.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHXWD_004204"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHXWD_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="ACHXWD_004205"
     CDS             2190..>3045
                     /locus_tag="ACHXWD_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR7622918.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          836 g          803 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcaccgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggagaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAZSBF010000344         3006 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZSBF010000344 DAZSBF010000000
VERSION     DAZSBF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.349"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            78..1679
                     /gene="aceB"
                     /locus_tag="ACHXWD_004206"
                     /pseudo
     CDS             78..1679
                     /gene="aceB"
                     /locus_tag="ACHXWD_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            1709..3000
                     /gene="aceA"
                     /locus_tag="ACHXWD_004207"
                     /pseudo
     CDS             1709..3000
                     /gene="aceA"
                     /locus_tag="ACHXWD_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT          752 a          779 c          823 g          652 t
ORIGIN      
        1 tgtgctgaac gaaaagcgca caacgatcct tcgttcacag tggggaagtt ttcggatcca
       61 tgacgaggag ctgcacgatg actgaacagg caacaacaac cgatgaactg gctttcacaa
      121 ggccgtatgg cgagcaggag aagcaaattc ttactgccga agcggtagaa tttctgactg
      181 agctggtgac gcattttacg ccacaacgca ataaacttct ggcagcgcgc attcagcagc
      241 agcaagatat tgataacgga acgttgcctg attttatttc ggaaacagct tccattcgcg
      301 atgctgattg gaaaattcgc gggattcctg cggacttaga agaccgccgc gtagagataa
      361 ctggcccggt agagcgcaag atggtgatca acgcgctcaa cgccaatgtg aaagtcttta
      421 tggccgattt cgaagattca ctggccccgg actggaacaa agtgatcgac gggcaaatta
      481 acctgcgtga tgcagttaac ggcaccatca gctataccaa tgaagcaggc aaaatttatc
      541 agctcaagcc caatccagcg gttttgattt gtcgggtacg cggtctacac ttgccggaaa
      601 aacatgtcac ctggcgtggt gaggcaatcc ccggtagcct gtttgatttt gcgctctatt
      661 tcttccacaa ctatcaggca ctgttggcaa agggcagcgg tccctatttc tatctgccga
      721 aaacccagtc ctggcaggaa gcggcctggt ggagcgaagt cttcagctat gcagaagatc
      781 gctttaatct gccgcgcggc gccatcaagg cgacgttgct gattgaaacg ctgcccgccg
      841 tgttccagat ggacgaaatc cttcacgcgc tgcgcgacca tattgttggt ctgaactgcg
      901 gtcgctggga ttacatcttc agctatatca aaacgttgaa aaactatccc gatcgtgtcc
      961 tgccagacag acaggccgtg acgatggata aaccattcct gaatgcttac tcacgcctgc
     1021 tgattaaaac ctgccataaa cgcggtgctt ttgcgatggg cggcatggca gcgtttattc
     1081 cgagcaaaga tgaagagcgt aataaccagg tgctcaacaa agtaaaagcg gataaagcgc
     1141 tggaagtcaa taacggtcac gatggcacat ggatcgctca cccaggcctt gcggacacgg
     1201 caatggcggt attcaacgac attctcggct cccgtaaaaa tcagcttgaa gtattgcgcg
     1261 aacaagacgc gccgattact gccgatcagc tgctggcacc ttgtgacggt gaacgcaccg
     1321 aagaaggtat gcgcgccaac attcgcgtgg ctgtgcagta catcgaagcg tggatctccg
     1381 gcaacggctg cgtgccgatt tatggcctga tggaagatgc ggcgacggct gaaatttccc
     1441 gtacctcgat ctggcagtgg atccatcatc aaaaaacgtt gagcaatggc aaaccggtga
     1501 ccaaagcctt gttccgctag atgctgggcg aagagatgaa agtcattgcc agcgaactgg
     1561 gcgaagaacg tttctcccag ggccgttttg acgatgccgc acgcttgatg gaacagatca
     1621 ccacttccga tgagttaatt gatttcctga ccctgccagg ctaccgcctg ttagcgtaaa
     1681 ccaccacata actatggagc atctgcacat gaaaacccgt acacaacaaa ttgaagaatt
     1741 acagaaagag tggactcaac cgcgttggga aggcattact cgcccataca gtgcggaaga
     1801 tgtggtgaaa ttacgcggtt cagtcaatcc tgaatgcacg ctggcgcaac tgggcgcagc
     1861 gaaaatgtgg cgtctgctgc acggtgagtc gaaaaaaggc tacatcaaca gcctcggtgc
     1921 actgactggc ggtcaggcgc tgcaacaggc gaaagcgggt attgaagcag tctatctgtc
     1981 gggatggcag gtagcggcgg acgctaacct ggcggccagc atgtatccgg atcagtcgct
     2041 ctatccggca aactcggtgc cagctgtggt ggagcggatc aacaacacct tccgtcgtgc
     2101 cgatcagatc caatggtccg cgggcattga gccgggcgat ccgcgctatg tcgattactt
     2161 cctgccgatc gttgccgatg cggaagccgg ttttggcggt gtcctgaatg cctttgaact
     2221 gatgaaagcg atgattgaag ccggtgcagc ggcagttcac ttcgaagatc agctggcgtc
     2281 agtgaagaaa tgcggtcaca tgggcggcaa agttttggtg ccaactcaag aagctattca
     2341 gaaactggtc gcggcgcgtc tggcagctga cgtgacgggc gttccaaccc tactggttgc
     2401 tcgtaccgat gccgatgcag cggatctgat cacctccgat tgcgacccgt atgacagcga
     2461 atttattacc ggcgagcgta ccagtgaagg cttcttccgt actcatgcgg gcattgagca
     2521 agcgatcagc cgtggcctgg cgtatgcgcc atatgctgac ctggtctggt gtgaaacctc
     2581 cacgccggat ctggaactgg cgcgtcgctt tgcacaagct atccacgcga aatatccggg
     2641 caaactgctg gcttataact gctcgccgtc gttcaactgg cagaaaaacc tcgacgacaa
     2701 aactattgcc agcttccagc agcagctgtc ggatatgggc tacaagttcc agttcatcac
     2761 cctggcaggt atccacagca tgtggttcaa catgtttgac ctggcaaacg cctatgccta
     2821 gggcgagggt atgaagcact acgttgagaa agtgcagcag ccggaatttg ccgctatacc
     2881 ttcgtatctc accagcagga agtgggtaca ggttacttcg ataaagtgac gactattatt
     2941 cagggcggca cgtcttcagt caccgcgctg accggctcca ctgaagaatc gcagttctaa
     3001 gcaacc
//
LOCUS       DAZSBF010000345         2992 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZSBF010000345 DAZSBF010000000
VERSION     DAZSBF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.350"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHXWD_004208"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHXWD_004208"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR7622919.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHXWD_004209"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHXWD_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR7622920.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHXWD_004210"
     CDS             2805..>2992
                     /locus_tag="ACHXWD_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR7622921.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZSBF010000346         2946 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZSBF010000346 DAZSBF010000000
VERSION     DAZSBF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.351"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            300..884
                     /locus_tag="ACHXWD_004211"
     CDS             300..884
                     /locus_tag="ACHXWD_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7622922.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            989..1729
                     /locus_tag="ACHXWD_004212"
     CDS             989..1729
                     /locus_tag="ACHXWD_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR7622923.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1764..2946
                     /gene="htrE"
                     /locus_tag="ACHXWD_004213"
                     /pseudo
     CDS             1764..2946
                     /gene="htrE"
                     /locus_tag="ACHXWD_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          855 a          613 c          615 g          863 t
ORIGIN      
        1 gtttaaacat ttttgcagaa acataatata aattcccgca gataaatatc aatcccatcg
       61 ccatacccta accaattgca cttgtgaatt tatttttgct taattttgaa ttaagaaaat
      121 ttaatttgtt caaaagccct taaagtgcac aataggcgtc ctaatgaaca atggttgtaa
      181 attttggtaa caaatatcat tgctcattaa tatctagcat ctgcttataa catttgtgtc
      241 atgaatagtc attcttcaaa ccagtagtct gactaatgga attttattca aggattttta
      301 tgtctaaaaa attaggtttt gccctgtctg gtcttatgct ggcaatggtt gcaggtactg
      361 cttctgctga tatggatggt ggtcagttaa atatcagtgg tctggttgtt gataacacct
      421 gtgaaacccg cgttgacggc ggtaataaag atggtctgat cctgctgcaa actgcaactg
      481 ttggtgaaat cgatgctggc gtgctgaatg acaccgttgg cgctaaagcc aaacctttca
      541 gcatcaccgt tgactgcagc aaagcaaatc cgaatccagg tagtacagct aaaatgacct
      601 tcggttctgt tttctttggt aatagcaaag gaacgctgaa caacgacatg tctattaaca
      661 acccgtctga cggcgtgaat atcgctctgc acaatattga tggttccact atcaaacagg
      721 ttcaaattaa caaccctggc gatgtgtata ctaaagccct ggatgcaacg acaaaatctg
      781 ctgtttatga ttttaaagcg tcttacgttc gtgccgtcgc agaccaaaca gcaactgctg
      841 gttatgtaaa aactaacact gcatacacca ttacttatca gtaagtattc accaattaaa
      901 ttgattgaat gaatatacag ggaatagtgt taataatttc tatttttata ttattccctg
      961 ttttaattaa ctctgtcagg gatggtttat gttttttaac accaaacata caacagcttt
     1021 atgcttcgta acctgtatgg cttttagttc atcgtctatt gcggacattg tcatttcggg
     1081 tactcgcgta atatataaaa gcgatcaaaa aagtgtcaac gtacgtctgg aaaataaagg
     1141 gaataacccg ttgcttgtcc agagttggtt agatactggc gataacaacg ctgagcctgg
     1201 cagtattaca gtccctttta ctgccacgcc gccagtatcg cgtattgatg ccaaacgtgg
     1261 gcaaacaatc aaattaatgt acacagccag cacctcactg cctaaagaca gagagagcgt
     1321 gttctggttt aacgtactgg aagttccacc aaaaccagat gcagaaaagg tcgcgaatca
     1381 aagtctgctg caactggcat ttcgcacacg tataaaactt ttctatcgcc cggatggatt
     1441 gaagggaaat ccctctgaag ccccgttagc cctgaagtgg ttctggtcag gttcagaagg
     1501 taaggcgtca ttgcgagtga ccaatccaac cccttactac gtctctttta gcagtggtga
     1561 tttagaagct agcggtaaac gctatccgat tgatgtgaaa atgattgcac catttagtga
     1621 tgaggtcatg aaagtcactg gccttaatgg caaagcaagt tctgcaaaag tacattttta
     1681 cgccattaat gattttggtg gcgccattga aggtaatgcc agtctgtaat caggcaagga
     1741 tataattccg caggaagcat aacgtgacta taaaaccaac taaaaattat cataatcatc
     1801 tgacccgtat cgctacgttt tgcgcgctgc tgtattgcaa tagtgctttc tgtgctgaac
     1861 ttgttgaata tgaccatacc ttcctgatgg ggcagaatgc ctcaaatatt gatctcagcc
     1921 ggtatagtga aggtaaccct gctataccgg gtatgtacga cgtcagtgtt tatgtcaacg
     1981 atcaacctat tattaaccaa agcattacat ttattgaaat tgaaggaaaa aaaacgcaca
     2041 agcctgtatc acacttaaga atttattgca gtttcatata aattctcccg atataaataa
     2101 cgaaaaagcc gttctgcttg ccagggatga aacactcggc aattgcctta atttgacgga
     2161 aattatccct caggcttctg ttcgttatga cgttaacgag caacgtctgg atatagacgt
     2221 tcctcaggca tgggtaatga aaaattacca aaactatgtt gatccatcgt tatgggaaaa
     2281 tggcattaat gcggccatgt tgtcatacaa cctcaacgga tatcatagtg aaactcctgg
     2341 tcgaagaaat gacagcattt atgctgcttt taacggtggg atgaatttag gtgcatggcg
     2401 actgcgtgcc tcaggcaact acaactggat gaccgattct ggcagtaatt atgattttaa
     2461 gaatcggtat atacagcgtg atatcgcctc gctgcgttct caactcattc ttggtgagtc
     2521 ttatacaacg ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt tatacagcga
     2581 cagccgcatg ttgcctccga cattagcgag ttttgcgcct atcattcatg gcgttgctaa
     2641 taccaacgcc aaagtcacta ttacgcaagg tggctataag atttatgaaa cgactgtgcc
     2701 gccaggcgct ttcgtcattg atgatctgag tccgtcaggt tacggcagcg atcttattgt
     2761 tactgtcgaa gaatccgatg gctcaaagcg gacattctcg caaccttttt catcggtcgt
     2821 tcaaatgtta cgccctggcg ttggacgttg ggatattagc ggcggccagg tcttaaaaga
     2881 tgatattcag gatgagccta atttatttca ggccagctac tactacggtc tgaataacta
     2941 tctaac
//
LOCUS       DAZSBF010000347         2944 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZSBF010000347 DAZSBF010000000
VERSION     DAZSBF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.352"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(193..570)
                     /locus_tag="ACHXWD_004214"
     CDS             complement(193..570)
                     /locus_tag="ACHXWD_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622924.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            complement(570..1007)
                     /locus_tag="ACHXWD_004215"
                     /pseudo
     CDS             complement(570..1007)
                     /locus_tag="ACHXWD_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1018..2154)
                     /locus_tag="ACHXWD_004216"
                     /pseudo
     CDS             complement(1018..2154)
                     /locus_tag="ACHXWD_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            complement(2294..>2944)
                     /locus_tag="ACHXWD_004217"
     CDS             complement(2294..>2944)
                     /locus_tag="ACHXWD_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR7622925.1"
                     /translation="DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAF
                     EKKFPLTELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDC
                     KIRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          779 a          720 c          473 g          972 t
ORIGIN      
        1 aaccatactt tttcataagg caatctaact tagctcttaa atcaccaaag cccatcacac
       61 cataacatct aatagccatt gccacacgtc actatggatt tatttccacc tgcaattact
      121 tcgtcctaac tttaatttaa tctcaagaag cattaagatc tcaatagcga gcttaatact
      181 tcttgaataa tatcactcct gaccaaaatt tttccacact aatgcatgag actttgattt
      241 gtcacccaga aatttactta aatcttgcca aagtaaagaa ttgctatcgg tcgcagtgac
      301 tattgcctct ataagccctg tagctacaac tgtggccaat tcatcatcaa gcgagaccat
      361 accatcttcg acatgtttaa atattttttc tctttcaata tcccccattt cataaaaatt
      421 ttttacaatc gtttttccaa cctgagagaa caacataacc accggtggcg tttctggatg
      481 ccagtattca ttagctaatg agactgtctc agacaaaaca gtggaaaatg atgccatttt
      541 ttctatataa ctagcaatat attggttcat tacttgcctc ccaaagcatt ctgtaaatcc
      601 tgaagaacgt tttctgctgc tgctcgatcc cctggagatg ctgttggatt cttatccaac
      661 caatcctgta aagcccttgt ataatcctgt gctttcaata catgtcctct accaccaact
      721 ttttcacctg tggccaattc ataacgaaca gcatctgctg tgcttccact tcctactttt
      781 gcatttggcc gataaagatc gtctataata tttttcaaat ttgaatctgt aacgtcgggt
      841 tttcccattt tctgtcgcaa ttcatcttta taccgttcat gctgcatacg atcccttgcg
      901 ctattctcat cttctggtcc ccatcctccc gggcttcccg tactgccgcc ggccaagcag
      961 ttcttctctt catccgtcaa atccttccca acattcggct gactccctag ggaaagatta
     1021 cgaaagcccg ctccccgcaa ggactgacgc gagatagttt ctatccatgg ctgcttttcg
     1081 catcttacgt cttaaccctg ccttgaatac cttatcattc gttaaaatat taatagcgat
     1141 gtgccgtatc cctgaaaata attctgcggc gtttcctctt cttattttgc agtcgtcttc
     1201 attcattacc acgtccagac gccagtgcag cttattctcc acgtgccagt ggtttcggat
     1261 tgctgtggcg aacttttctg cggttaaatc agcagaactg atataatatc tgaccgtcat
     1321 ttctggctct ttcttttgtt ctgctattat tgaccgaaag gagactgcca cgcataattt
     1381 cttcagtcct ttccattcaa acgtgaaatc aataagttca tcagggacat cgcaaacaat
     1441 atgaagacgg atttcttctc tgccgtgact cttttcactc attgcgtaac tgtcatgctc
     1501 tggattattt aattctttca gcggaaattt ttcctcaaag gctttattta gccgcccctg
     1561 gtttcctttt accgcgaata aataatcacc tccctgtttt tgtatcttct ctgcaatatc
     1621 tttctggcaa cccatcgcat cagttgtgat gatttttcct ttaatatcta gcatgttaag
     1681 aagttctggg atagctgtaa tctcattaga tttctcatcc gtcttgatct gtccgatgac
     1741 cagactgtgc attgttgaga acgcactaat gacatgaatc gctcccctgc ggcgactctt
     1801 gtcataagag tgccggagcg tttttccatc aattgcaatg acgtctttat catctgaaga
     1861 atggcagtca cgcattcagt taataaagca ctcgtgaaat tttgcaggac tgatacagga
     1921 tacaactctg gcaatggtat cgtgaacagg aataccattt tcaaaatcac catattgctt
     1981 caaaaaatcg agatgtgttt ccccaaaatc ctctatatct tcccaaccct ctgcaccaga
     2041 aataacggca caaatagtca acagtagaat atccgataat ttatgttcca ctttccaggc
     2101 ttgtctgtaa tcggggataa tagaaatatg ttccatcaat tttttaagtt ccattttgtt
     2161 ctccttaatt atgtaaggag tatttgatca tgtataagca ataaaaaaca gcttcaggta
     2221 ataaggaata tctcaatttt taaacataaa atgcgaatta tttagtacaa aaagtgaact
     2281 cctcagggca agattacgaa agtccgctcc ccgcaaggac tgacgcgaga tagtttctgt
     2341 ccatggctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     2401 aaacattaat agcaatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     2461 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagatta ttctccacgt
     2521 gccagtgatt tcgaatcgct gtcgcaaact tctcagcggt taaatcagca gaactgatat
     2581 aatatctgac catcatttcg ggctctttct tttgctctgc tattattgag cgaaaggaga
     2641 ctgccacgca taattttttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     2701 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactcattg
     2761 cggaactgtc atgcgctgga ttatttaatt ctgtcagcgg aaattttttc tcaaaggctt
     2821 tatttagccg cccctggttt ccttttaccg caaataaata atcacctccc tgtttttgta
     2881 tcttctctgc aatatctttc tggcaaccca tcgcatcagt tgtgatgatt tttcctttaa
     2941 tatc
//
LOCUS       DAZSBF010000348         2944 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZSBF010000348 DAZSBF010000000
VERSION     DAZSBF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.353"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            45..1120
                     /locus_tag="ACHXWD_004218"
                     /pseudo
     CDS             45..1120
                     /locus_tag="ACHXWD_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1136..1884
                     /locus_tag="ACHXWD_004219"
                     /pseudo
     CDS             1136..1884
                     /locus_tag="ACHXWD_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            1886..>2944
                     /locus_tag="ACHXWD_004220"
     CDS             1886..>2944
                     /locus_tag="ACHXWD_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622926.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT          932 a          587 c          613 g          812 t
ORIGIN      
        1 gggcaactgg acgtcaaggt tgaagaagag gacgggccag tctatatatc aatgacacta
       61 accattacat gaattatcag aatgaaaaag agagttatat tgtcacgttt aaccagtatc
      121 ttgaatcatt aaggttaaat acatacgtaa gtttggctcg taatacttac tgggacgcca
      181 gcagtaatgt gaattattca ttatcactta gccgcgattt tgatatcggg ccattaaaaa
      241 acgtctccgc ttctctaaca tttagccgaa taaactggga agaagacaac caggatcaac
      301 tgtacctaaa tatttcgatt ccctggggaa ccagtagaac attgagctat ggtatgcaac
      361 gaaatcagga taacaagatt tcgcatactg cttcgtggta tgactcttcc gatcgaaata
      421 attcctggag cgtttctgct tcaggcgaca atgatgaatt caaagatatg aaagcgtcac
      481 tacgcgccag ttatcagcat aataccgaga acggtcgtct ctatctctcc gggacatcac
      541 agcgagacag ttattattct ctgaatgcca gttggaatgg ttcattcacg gcgactcgtc
      601 acggagccgc tttccatgac tatagtggta gtgctgactc gcgttttatg atcgacgcag
      661 atggtgcaga agatattccg ttgaacaata aacgcgcggt aactaatcgg tatggcatcg
      721 gagttattcc atcagtcagc agttacataa caacatcatt aagcgttgat acccgaaatc
      781 ttccagaaaa tgtggatatc gaaaactcgg ttatcaccac caccttaacc gaaggtgcta
      841 ttggctacgc caaacttgat accccaaggg ctatcaaatc atgggggtta ttcgcctggc
      901 agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc ataaagaatt
      961 aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg acagcaaact
     1021 tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca gcaacttaac
     1081 caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga aactaatgcg
     1141 ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca tgagcagcat
     1201 tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt cagataaagg
     1261 ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc aaacatggat
     1321 agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc ctatggtacg
     1381 tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa ttgctcaact
     1441 gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc caaaaacaga
     1501 taaaaaaatg tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa
     1561 gctattgaat tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga
     1621 aatgatggtt cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc
     1681 ggaacaaacg gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc
     1741 catgcagata cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat
     1801 tttggtggat taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt
     1861 tccgtagcca aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat
     1921 aggttttagt gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa
     1981 taccgtagtc attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga
     2041 taagatctgg caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc
     2101 cccatcacct ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg
     2161 cggtcaggac ttttataatc agaactccta tatttttggc gtaacataca atggagttga
     2221 ttatgattcg actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc
     2281 gggtaccttt aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca
     2341 attatcagta acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa
     2401 tagagttaat aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat
     2461 gagtggcgca ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa
     2521 taatattcat gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt
     2581 tgactttggt aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag
     2641 tgtagtcatg acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag
     2701 ctttttcact acggatattt tgagtgatta tagccatctg gatatgggta atggactgct
     2761 attgaagata tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc
     2821 ttctttttca tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc
     2881 gaacccggca gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac
     2941 ctat
//
LOCUS       DAZSBF010000349         2939 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZSBF010000349 DAZSBF010000000
VERSION     DAZSBF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.354"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHXWD_004221"
     CDS             37..264
                     /locus_tag="ACHXWD_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR7622927.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHXWD_004222"
     CDS             complement(265..639)
                     /locus_tag="ACHXWD_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR7622928.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHXWD_004223"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHXWD_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHXWD_004224"
     CDS             complement(2099..2674)
                     /locus_tag="ACHXWD_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7622929.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          696 c          688 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt agatttgttg tctatagcta
     2761 tagtgactct tctatgtaga cattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZSBF010000350         2910 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZSBF010000350 DAZSBF010000000
VERSION     DAZSBF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.355"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..359
                     /locus_tag="ACHXWD_004225"
     CDS             <1..359
                     /locus_tag="ACHXWD_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR7622930.1"
                     /translation="GRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGE
                     RSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLR
                     KDIMRRLTQDVCEEIE"
     gene            complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHXWD_004226"
     CDS             complement(416..2794)
                     /gene="ppsA"
                     /locus_tag="ACHXWD_004226"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR7622931.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          699 a          797 c          727 g          687 t
ORIGIN      
        1 ccggacgcaa aaaagatatt attgtccgcg gcggcgaaaa tattagcagc cgtgaagtag
       61 aagatatttt attgcagcat cctaaaattc acgatgcctg tgtggttgca atgcccgatg
      121 aacgtttagg tgaacgatca tgcgcttatg tcgtgctgaa agcgccgcat cattcattat
      181 cgctggaaga ggtggtggct ttttttagcc gtaaacgggt cgcaaaatat aaatatcctg
      241 aacatatcgt ggtaatcgaa aaactaccgc gtactgcctc aggtaaaata caaaagtttt
      301 tgttaagaaa agatattatg cggcggttaa ctcaggatgt ctgtgaagag attgaataag
      361 tttcatcttc ggggatcaca taaccccggc gactgaacgc cgccggggat ttattttatt
      421 tcttcagttc agccaagctt aaccaggttt gcaccacggt gtccgggttc agagacaggc
      481 tatcgatccc ctcttccatc aaccatgcgg caaagtcttc gtggtcggac ggaccctgac
      541 cgcaaatccc gacatatttg ccctgtttct tcgcggcacg gatagccatc gacagcagtg
      601 ctttcaccgc atcgttgcgc tcatcgaaca attcagacac cacgccggag tcacggtcca
      661 gaccgagcgc cagctgcgtc atatcgtttg agccaattga gaagccgtcg aaatattcga
      721 ggaactgctc ggccagcaag gcgttggacg ggatttcaca catcatgatg attttcagcc
      781 cgttctcacc acgcttcagc ccctgacgcg ccagttcctc aaccaccgct ttcgcctgat
      841 ctacggtacg cacgaacggg atcatgatct caacgttggt cagccccatg tcgttgcgca
      901 cacgtttcac tgcttcgcac tccagcgcga agcagtcgcg gaagctgtcg gaaacatagc
      961 gtcccgcgcc acggaagccg agcatcgggt tctcttcatc tggctcgtaa cgctcaccac
     1021 cgaccaggtt ggcatattcg ttcgatttaa aatcagagag acggacaatg acgcgcttcg
     1081 gataaaacgc ggcacccagc gtcgcgatcc cttcagtcag acgaccaacg taaaattcac
     1141 gcggagaatc aaaacctttc atcatctcgc ggatttcatt ttgcaactgc ggttcctgat
     1201 cgtcaaactc aagcagtgcg cgtgggtgga cgccaatcat acggttgatg ataaattcca
     1261 gacgcgcaag gcccacgcct tcgttcggca ggcaggcgaa gtcgaaagca cggtccgggt
     1321 taccgacgtt catcatcact ttcaacggca gatccggcat catttctacg ctggagcttt
     1381 tcacgctaaa ttccagcaac tccgcataga cgtaaccggt atcaccttcg gcacaagaaa
     1441 cagtgacgtt ctcaccgtct ttcatccgtt ctgttgcatc accacagccc actaccgccg
     1501 gaatgcccag ttcacgagcg atgatcgccg cgtgacaggt acgaccgcca cggttggtga
     1561 cgatggcaga tgctttcttc atgatcggtt cccagtccgg gtcggtcatg tcagtgacca
     1621 gcacgtcacc aggttcgatg cggttcattt cgctgatctc atggatgact ttcaccggac
     1681 ccgcgccgat gcgatgaccg atagcacggc cttcggcgat aatcttaccc tgtgaatgca
     1741 gcgtataacg ctccatgacc tgaccgcgtg agcgcacggt ttccggacgc gcctgcacaa
     1801 tgaacagttt gccagtgtgg ccatctttcg cccactcaat atccatcggg cgaccgtagt
     1861 gtttctcaat ttgtacggcc tgttttgcca gttcctgcac ttcttcgttg gtcagcgaga
     1921 agatgtcacg ctgttcctgc ggtacgtctt cgattttaac ctgcttgccg tgctcctggg
     1981 tcggcgcgta aaccatgcgg atttttttcg accccatggt gcgacgcacg atagccgggc
     2041 gattcgccgc cagtgtcggt ttatggacgt aaaactcatc cgggttaacc gcaccctgca
     2101 cgaccatttc cccaaggccc catgcggaag tgataaacac cacctggtca aagccggatt
     2161 cggtatcaat ggagaacatc acgccagaag atgcgaggtc ggagcgcacc atccgctgaa
     2221 caccggcaga gagcgccacg ccacggtgat cgtaaccctg gtgcacacga taagagatgg
     2281 cgcgatcgtt aaacagagaa gcaaatacat gcttcactgc cacgagaacg gcgtcaaaac
     2341 cctgaacgtt gaggaaggtt tcctgctgac cggcaaaaga agcgtccggc atatcttctg
     2401 cggtggcgga ggagcgcacc gcaaaagagg cgttttcgtc atcggcggaa agctgtgcat
     2461 aggcttcgcg gatggcgttt tccagctcag gctggaaggg agtgtcgata atccactggc
     2521 ggatttgcgc gcccgctttc gcaagctgag taacatcgtc aatatccgtt ttatccagca
     2581 gttcataaat gcgctggttt acgccgcttt ggtccagaaa ctggttaaac gcgtcggcgg
     2641 ttgtggcgaa accattcgga acggaaacac ccattccgga aaggttagta atcatttcac
     2701 ccagggaggc atttttgccc ccaaccctgt ctacatcatt catgccgagt tggttatacc
     2761 aaagcaccag cggtgacgag ccattgttgg acatcgaaca atccttttgt gataaatgaa
     2821 cggtttgaga aacacatttc tgcgcattta tctttgcata tttaaccgga tgaaaaaaac
     2881 ggtgaatcgt tcaagcaaat atattttttt
//
LOCUS       DAZSBF010000351         2888 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZSBF010000351 DAZSBF010000000
VERSION     DAZSBF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.356"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            104..736
                     /gene="comR"
                     /locus_tag="ACHXWD_004227"
     CDS             104..736
                     /gene="comR"
                     /locus_tag="ACHXWD_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR7622932.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(798..1337)
                     /locus_tag="ACHXWD_004228"
     CDS             complement(798..1337)
                     /locus_tag="ACHXWD_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR7622933.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1564..2868)
                     /gene="ndh"
                     /locus_tag="ACHXWD_004229"
     CDS             complement(1564..2868)
                     /gene="ndh"
                     /locus_tag="ACHXWD_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR7622934.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          699 a          784 c          703 g          702 t
ORIGIN      
        1 agttaaatag aaagcaatta tttttatggg atctggactg gtgaacggag gtgattattt
       61 ttataataac ctttcattca cagtcaccag gttcaacggc atcatggcaa ctgactcaac
      121 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      181 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      241 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      301 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      361 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      421 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      481 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      541 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      601 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      661 cgaaggtgca tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc
      721 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      781 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc
      841 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      901 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      961 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
     1021 ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc accaaactga
     1081 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1141 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1201 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg
     1261 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1321 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1381 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1441 ggattcggag aagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta
     1501 ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc cagtgtacgt
     1561 cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat
     1621 taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata
     1681 tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag
     1741 gctaccgaca gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata
     1801 atttttcagc ggtttaccgt tcatctgcgc cagaatattt ttcattgcgc aggtcgccat
     1861 ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc
     1921 gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg gttccaccac
     1981 cagctggttg atacggttgg tttcaagacc accgatatct ttcaggaagt ctggcgcttt
     2041 gatcccggct gcccacacca tcagatcagc ctcaatatat tcaccatctt tagtgtgcag
     2101 gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt
     2161 tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc
     2221 cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg
     2281 cagttgcttg accgcattgt acaattcagc ggagagttct acacccgtcg cgccgccgcc
     2341 gacaatcgca atgttcactt taccattcgc gcccaggttg gcggagtatt tcaggaacag
     2401 attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt
     2461 ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca ttaccagggt
     2521 gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc gcagttctgc
     2581 aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct ggaactggaa
     2641 accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc
     2701 agtcgccact tcgtgcagca gcggtttcca cagatggctg tggttacgat cgaccagcgt
     2761 aattttggct tttttcttgc gtcccagctt atgccccagc tgtgttgcca tttccagccc
     2821 accagcaccg ccgccgacaa tcacaatttt tttcaatggc gtagtcaacg tgaccccctt
     2881 aaatttat
//
LOCUS       DAZSBF010000352         2877 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZSBF010000352 DAZSBF010000000
VERSION     DAZSBF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.357"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHXWD_004230"
     CDS             complement(<1..953)
                     /gene="curA"
                     /locus_tag="ACHXWD_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR7622935.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDG"
     gene            1134..1652
                     /gene="mddA"
                     /locus_tag="ACHXWD_004231"
     CDS             1134..1652
                     /gene="mddA"
                     /locus_tag="ACHXWD_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR7622936.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1649..2098
                     /locus_tag="ACHXWD_004232"
     CDS             1649..2098
                     /locus_tag="ACHXWD_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR7622937.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHXWD_004233"
     CDS             complement(2099..2332)
                     /gene="ydcY"
                     /locus_tag="ACHXWD_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR7622938.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHXWD_004234"
     CDS             complement(2418..2591)
                     /gene="ortT"
                     /locus_tag="ACHXWD_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR7622939.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHXWD_004235"
     CDS             2786..>2877
                     /gene="yncL"
                     /locus_tag="ACHXWD_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR7622940.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          756 t
ORIGIN      
        1 aaaccatcag taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc
       61 tccttctgaa actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag
      121 cgaatacgtt ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt
      181 agctctgtag cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta
      241 agtaatggta gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata
      301 ccttttgggc acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa
      361 acatcgaagc ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc
      421 accaccctgc aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc
      481 gccgcagcta ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata
      541 taagcggtaa agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga
      601 tcgccaagtt tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag
      661 cccagcaccc agtcgctagg ctgataatca ggatgattcg actccacaac acggctcacc
      721 gtgccgccga ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc
      781 atacgtccac gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct
      841 tcacccggtg tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca
      901 tgtggacgcg aggccagaac ccaacgtcga ttacgctgct tttgttgccc cataatgctc
      961 tcctgtgctt ttggcatgaa tccatcaata gtagcaggct cacgggattg gttttgtttg
     1021 cagccgtcac atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg
     1081 ctgttttcga tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca
     1141 tccgttttgc ccgcaaagcc gattgtgctg ccattgcgga aatttataac cacgccgtgt
     1201 tgtatacggc ggctatctgg aatgaccaaa cggttgatgc agataaccgc attgcctggt
     1261 ttgaagcgcg gaccatagca ggttatccgg tgctggtaag tgaagaagat ggcgtagtga
     1321 cgggatatgc ctcgtttggc gactggcgta gtttcgatgg ttttcgccat accgtggaac
     1381 attcggttta tgtccatccc gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagcc
     1441 gattgattga tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc
     1501 aaaatcaggc ctcgctgcat ctgcaccatt cgctgggatt tgtcgtcacc gcacaaatgc
     1561 cgcaggtggg cactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg
     1621 acgaacgcac tgaaccggac gcgattggat gaatcagtcg ctcacccttg cttttttgat
     1681 tgccgccgga attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc
     1741 ctctaccatt ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc
     1801 tattttatgg tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg
     1861 gtggacactt atacccggct tgctgggatc gttttttgtc ttcgccagta tcagcggata
     1921 tcagaatgtg ggagcggcga caactattgc agtactggtc gccagccagc taattggcgg
     1981 tctgatgctg gatatcttcc gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat
     2041 ttgtggcgca attttacttg tggttggcgc ctggttggta gccagacgct cattttaatc
     2101 agaggatggt gccgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgct
     2161 tgcgaccgtg atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc
     2221 gacttaactc cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc
     2281 tctcttcaat cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc
     2341 cttaaccact tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc
     2401 aagccagtga gtaagattta aaacagtgaa aacaacagcg ccaccgggaa actcatcggc
     2461 catgttgccc ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga
     2521 aaccaggtga taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga
     2581 tagagagaca tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt
     2641 ttttgacgtg catcaagttt tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc
     2701 ccttatccat cttcagaatg agtattattg cggattttct taacccgtac tatacacata
     2761 ggggcaatga taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa
     2821 tgtctttgct gctgttggtt tgtttactct tatctctatg agatttggct ggtttat
//
LOCUS       DAZSBF010000353         2876 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZSBF010000353 DAZSBF010000000
VERSION     DAZSBF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.358"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(29..1484)
                     /gene="xylB"
                     /locus_tag="ACHXWD_004236"
                     /pseudo
     CDS             complement(29..1484)
                     /gene="xylB"
                     /locus_tag="ACHXWD_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(1556..>2876)
                     /gene="xylA"
                     /locus_tag="ACHXWD_004237"
     CDS             complement(1556..>2876)
                     /gene="xylA"
                     /locus_tag="ACHXWD_004237"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR7622941.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          684 a          845 c          668 g          679 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc cgtacggtaa
      241 tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact acgcgccccg
      301 ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac atccatgcca
      361 tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt ggggccatgt
      421 tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg tgtacgctcg
      481 ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg ttgagctgca
      541 gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc cagacacgat
      601 gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg gcaaaagctg
      661 tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata gacccccgac
      721 gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac tgcaccagct
      781 gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact
      841 tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga
      901 gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac atccagccac
      961 atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagacgcaag
     1021 taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc atgccgctga
     1081 acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt aatcactcgt
     1141 gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac
     1201 aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc atgcatctgg
     1261 ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag agctttcatt
     1321 gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag tggatgcggg
     1381 cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc gttgagcaaa
     1441 ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa
     1501 aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt agccgttatt
     1561 tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca
     1621 ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt
     1681 tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg atgcgtttat
     1741 ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc atcgtatcca
     1801 tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg
     1861 catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca
     1921 gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc
     1981 cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat
     2041 ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta atctcttttt
     2101 ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca tattgatgtt
     2161 tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg attttatgtt
     2221 tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga cgcaagtcgg
     2281 tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca
     2341 atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg aagacttcag
     2401 gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag ttagcggttc
     2461 cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca accatttgcg
     2521 caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc acatcgtgga
     2581 agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct
     2641 tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc cccacaccaa
     2701 acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct
     2761 cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat gctaacgggt
     2821 ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgc
//
LOCUS       DAZSBF010000354         2797 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZSBF010000354 DAZSBF010000000
VERSION     DAZSBF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.359"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..617
                     /locus_tag="ACHXWD_004238"
                     /pseudo
     CDS             1..617
                     /locus_tag="ACHXWD_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
     gene            614..1093
                     /locus_tag="ACHXWD_004239"
     CDS             614..1093
                     /locus_tag="ACHXWD_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR7622942.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="ACHXWD_004240"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="ACHXWD_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR7622943.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2249..2305)
                     /gene="ysgD"
                     /locus_tag="ACHXWD_004241"
     CDS             complement(2249..2305)
                     /gene="ysgD"
                     /locus_tag="ACHXWD_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR7622944.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2467..>2797
                     /locus_tag="ACHXWD_004242"
     CDS             2467..>2797
                     /locus_tag="ACHXWD_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622945.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          843 a          620 c          590 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttccctta tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAZSBF010000355         2756 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZSBF010000355 DAZSBF010000000
VERSION     DAZSBF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.360"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(362..1069)
                     /locus_tag="ACHXWD_004243"
     CDS             complement(362..1069)
                     /locus_tag="ACHXWD_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622946.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVAS
                     PELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQY
                     RAQYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRS
                     TGERIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTV
                     VALHFRFKEDKQIKMTI"
     gene            complement(1602..1856)
                     /gene="mobC"
                     /locus_tag="ACHXWD_004244"
     CDS             complement(1602..1856)
                     /gene="mobC"
                     /locus_tag="ACHXWD_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR7622947.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            2035..>2756
                     /gene="mobQ"
                     /locus_tag="ACHXWD_004245"
     CDS             2035..>2756
                     /gene="mobQ"
                     /locus_tag="ACHXWD_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR7622948.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALVNAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTEL"
BASE COUNT          731 a          696 c          611 g          718 t
ORIGIN      
        1 ctgggtttta atcgtacctg ggacgaatgg cggcgcgagg aagatgcacg aacacacgcc
       61 accgagagga gacatgatcg cggttggtca cagtgaatgt gaacaatatt ttgttttgtt
      121 taacatcgtg ttatgttcgt cgatgttctt cgatgttctt cgatgttcat cgacagggat
      181 gaatgcaaga agcacacaca taatgcaagc atagttttgt ttttccgccc ccaaaaaatg
      241 ggggttcttt tttcgctgca ttagagcgct tttgacgcaa tttttcactt caatagtacc
      301 ttcgcactac taacacaaaa aaaacgcagc agggagcgtt ttaggtgcct taaaatcgat
      361 tttagatcgt catcttgatt tgtttgtctt ccttgaagcg aaaatgcagg gctacaaccg
      421 tgcgcccctt tttgatcgtc tcaaccgtta cagccaggtc gctttttttg ttcagttcgt
      481 caacacaggg ttttattaga tccctgttta acgttttgaa atctttatat ttaccatcaa
      541 gcatcagtga tgatttaaaa tcatcgaggg cgataatacg ttccccagta ctgcggaatt
      601 gctggagaat ttcgtatatt cttatgctgt aacttcttga taaattagaa atatttttaa
      661 tgacgactct ggtgaattgc cccttcaact gtgtcagata aggcattacc gcgtcagaaa
      721 aagtgatttg tgctttcccc tctcccctcg cgtactgtgc ccgatactga atccagcgga
      781 actcctcgcg cttctcgtcg tccttgagaa tgatcgacct gtcccacaaa cgatcgatcg
      841 cttcctgtag ctgaacctcg gcatttttcc gccccatatc agggaatgaa tcaaaatatt
      901 ccgcagctgt aatggtcatt gtcttttgca attcaggtga agctacgtca gtaccggagt
      961 taagtcggct tatgcagagc aataagaaac gctgttcttg taaagttagc ttgtaactgg
     1021 cctcaaccag cgcattggac ttataggcta tcagagggtt tgatgtcatt ttaaccacca
     1081 ggccaaaaat gaggtgaaat aaaaacctat cacctttagc attggtgagc aattatattt
     1141 tcacccccca ttttcctgtg aatattacct taaatctcct cacctttacc ttaaattacc
     1201 tcacccaagc accttaaatt atatcacctt taccttaaat catattacct atagcattgt
     1261 aacattttgt tttctataag gaattttggc gctaaaacaa gtattaaaaa caagatctta
     1321 aaaacaagat ctaaaaactt aacgacgagc ctgtggataa cttggagtgc tgaaaacaaa
     1381 aaaccaataa aaacaatgcg ttgcaaaaaa agcagaattg caagttccga atacaccccc
     1441 tccaaccctg tggaaatcca gtattcatgc gggttcagcg catatcacct cccgaaaaac
     1501 cgtgcgtatg tgctcaatca ggcgacggat tcacttccgc ttcctcgccc actcgctcgc
     1561 taacgctcgg tcgctgggac tgcggcgagc ggacgataca atcactgtcc tggcagcgct
     1621 tccagctcgt taatcagcgg cagtatgcgc gtcacatcag cctgtcgtgt cacctctgcc
     1681 ttcaggaact ggagtaacca ggcacgcaac tgcacctcct ccggctttct gacctttgcc
     1741 agcaatgctg cacccacaat gaccttctgc gctgtgtcca gactcctgct cttcgccttc
     1801 aggcgctgta atctggcctc agcatcagca atcttctgtt cgagtgttct gctcatgatg
     1861 taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca ctggtagttt aaaaactgag
     1921 ctggcataat gcacggaaaa tcacgaagtg cgcacttata caatctccac tccgtttcga
     1981 ttgtgtgcgc cctgcggggc tgaaagaaaa ccacaaaaag gcattacggc agaaatggcg
     2041 atttatcatc tcagcatgaa aatcatctcc agaaacagcg gctacagcgc cgttgcttct
     2101 gctgcctatc gttccggttc actgatgctt gatgagcgta ccggactgac tcacgactac
     2161 acccgcaaaa gcggtgtggc tgaagctgtg attctgactc ccgcgacagc ccctgcctgg
     2221 tgtacgaacc gtgccgaact ctggaatgcc gttgagaagg ccgaacgccg gaagaactca
     2281 caacttgcgc gggaaattga actcgccatc ccccgcgaac taccgcagga tgccgcacgg
     2341 gaaaccgttc tcgcctttgt cagagaaaac tttgtcagtc agggcatgat tgctgatgtg
     2401 gcgttccatc acatggataa aacaaatccc catgcgcaca tcatgctgac cacgagagct
     2461 gtcggacctg ctggattcgg tggaaaggtc agggactgga acgaccggac acatgccgag
     2521 acgtggcgtg catcatgggc tgaccatgcg aacagggcac tggtgaacgc cggttatcag
     2581 gaagagatag accatcgttc atacgagcga caggggcttg agaaaacgcc tggtattcac
     2641 ctcggaaagt ctgcctgtgc gatggaaaca cgcggtatcg aaacagaacg cggcgaacag
     2701 aatcgtctga ttaacagact taacctggaa atacagattt cccgtaccga actcag
//
LOCUS       DAZSBF010000356         2730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZSBF010000356 DAZSBF010000000
VERSION     DAZSBF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.361"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..722)
                     /gene="mobQ"
                     /locus_tag="ACHXWD_004246"
     CDS             complement(<1..722)
                     /gene="mobQ"
                     /locus_tag="ACHXWD_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFR7622949.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTR
                     KSGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTH
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETE
                     RGEQNRLINRLNLEIQISRTEL"
     gene            901..1155
                     /gene="mobC"
                     /locus_tag="ACHXWD_004247"
     CDS             901..1155
                     /gene="mobC"
                     /locus_tag="ACHXWD_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="HFR7622950.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRK
                     PEEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
     gene            1687..2379
                     /locus_tag="ACHXWD_004248"
     CDS             1687..2379
                     /locus_tag="ACHXWD_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622951.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQK
                     TMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYA
                     KGEAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERI
                     IALNDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHF
                     RFKEDKQIKMTI"
BASE COUNT          708 a          596 c          702 g          724 t
ORIGIN      
        1 ctgagttcag tgcgggaaat ctgtatttcc aggttaagcc tgttaatcag acgattctgt
       61 tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac aggcagactt tccgaggtga
      121 ataccaggcg ttttctcaag cccctgtcgc tcgtatgaac gatggtctat ctcttcctga
      181 taaccggcgt tcgccagtgc tctgtttgca tggtcagccc atgatgcacg ccacgtctcg
      241 gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga acccagcagg tccgacagcc
      301 ctcgtggtca gcatgatgtg cgcatgggga tttgttttat ccatgtgatg gaacgccaca
      361 tcagcaatca tgccctgact gacaaagttt tctctgacaa aggcgagaac ggtttcccgt
      421 gcggcatcct gcggcagttc acgggggata gcgagttcaa tttcccgcgc aagttgtgag
      481 ttcttccggc attcggcctt ctcaacggca ttccagagtt cggcacggtt cgtacaccag
      541 gcaggggctg tcgcgggggt cagaatcaca gcttcagcca caccgctttt gcgggtgtag
      601 tcgtgagtca gtccggtacg ctcatcaagc atcagtgaac cggaacgata ggcagcggaa
      661 gctacggcgc tgtagccgct gcttctggag ataattttca tgctgagatg ataaatcgcc
      721 atttctgccg taatgccttt ttgtagtttt ctttcagccc cgcagggcgc acacaatcga
      781 aacggagtgg agattgtata agtgcgcact ttgtgatttg tcgcgcatta tgccagctca
      841 gtttttaaac taccagtgac gcgctaaaat gcgatttttc atcgcgcacg gagttacatc
      901 atgagcagaa cgctcgaaca gaagattgct gatgctgagg cgagattaca gcgcctgaag
      961 gcgaagagcc ggagcctgga cacagcgcag aaggttgttg tgggtgcagc attgctggca
     1021 aaggtcagaa agccggagga ggtgcagttg cgtgcctggt tactccagtt cctgaaggca
     1081 gaggtgacac gacaggctga tgtgacgcgc atactgccgc tgattaacga gctggaggcg
     1141 ctgccaggac agtgattgta tcgtccgctc gccgcagccc cagcgaccga gcgttagcga
     1201 gcgagtgggc gaggaagcgg aagtgaatcc gtcgccaggt tgagcacata cgcacggttt
     1261 ttcggtaggt gatatgcgct gaacccgcat gaatactgga tttccacagg gttggaaggg
     1321 gagtattcgg aacttgcaat tctgctttct tgcaacacat tgtttttatt ggttttttgt
     1381 tttcagcgcc ccaagttatc cacaggctcg ccgttaagtt tttagatctt gtttttaaga
     1441 tcttgttttt aatacttgtt ttagggtcaa aattcattaa taaaatcaat atgttataga
     1501 ggtatatgtg gaggaattta aggtaaaggt ggaggaattt aaggtgttta ggtggaggaa
     1561 tttaaggtaa aggtggagga atttaaggtg ttattcacag aaatgtgagg atataaaaaa
     1621 aaatattcca catttcttat ttgtggggca cattaaagca tcaccacatt tgaacgggat
     1681 ctttgtatgt cggacttgat tgcctacaag tcgaatgctt tggttgaggc cagctacaag
     1741 ttgaccctac aggaacaacg ttttctgttg ctgtgtatca gtcgattgaa atctggtgct
     1801 gatgctgaat tgcaaaagac aatgaccatt accgctgcgg aatattttga ttcattccct
     1861 gacatggggc ggaaaaatgc cgaggttcag ttacaggaag cgatcgatcg tttatgggac
     1921 aggtcgatca ttctcaagaa tgacgagaag cgtgaggagt tccgctggat ccagtatcgg
     1981 gcacagtacg ctaaaggtga ggccaaggct cgaataacct tttctgacgc ggtaatgcct
     2041 tatctgacgc aactgaaggg gcaattcacg cgcgttgtta taaaaaacat atcaggttta
     2101 tcaagtagtt acagtataag aatatacgag ttcctccagc aattccgcag tacgggagag
     2161 agaattattg ctcttaatga ctttcgttca atgttaggga ttgaaaataa atacaaacaa
     2221 ttcagagact taaataaaat actaataaaa ccctgtgttg acgaactgaa caaaaaaagc
     2281 gacctggctg taacggttga gacgatcaaa aaggggcgca cggttgtagc cctgcatttt
     2341 cgcttcaagg aagacaaaca gatcaagatg acgatctaaa atcgatttta aggcacctaa
     2401 aacgctccct gctgcgtttt ttttgtgtta gtagtgcgaa ggtactattg aagtgaaaaa
     2461 ttgcgtcaaa agcgctctaa tgcagcgaaa aaagaacccc caatttttgg gggcggaaaa
     2521 acaaaactat gcttgcatta tgtgtgtgct tcttgcattc atccctgtcg atgaacatag
     2581 aagaacatcg acgaacataa cacgatgtta aacaaaacaa aatattgttc acattcactg
     2641 tgaccaaccg cgatcatgtc tcctctcggt ggtgtgtgtt cttgcatctt cctcgcgccg
     2701 ccattcatcc caggtacgat tgaaacccag
//
LOCUS       DAZSBF010000357         2727 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZSBF010000357 DAZSBF010000000
VERSION     DAZSBF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.362"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHXWD_004249"
     CDS             complement(423..680)
                     /locus_tag="ACHXWD_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622952.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHXWD_004250"
     CDS             complement(677..1174)
                     /locus_tag="ACHXWD_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR7622953.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHXWD_004251"
     CDS             complement(1376..1834)
                     /locus_tag="ACHXWD_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR7622954.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHXWD_004252"
     CDS             complement(1831..2328)
                     /locus_tag="ACHXWD_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR7622955.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHXWD_004253"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHXWD_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR7622956.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          708 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataacc
//
LOCUS       DAZSBF010000358         2725 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZSBF010000358 DAZSBF010000000
VERSION     DAZSBF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.363"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(87..1160)
                     /gene="lpfD-O113"
                     /locus_tag="ACHXWD_004254"
     CDS             complement(87..1160)
                     /gene="lpfD-O113"
                     /locus_tag="ACHXWD_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR7622957.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(1171..>2725)
                     /gene="lpfC-O113"
                     /locus_tag="ACHXWD_004255"
     CDS             complement(1171..>2725)
                     /gene="lpfC-O113"
                     /locus_tag="ACHXWD_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR7622958.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT          753 a          642 c          545 g          785 t
ORIGIN      
        1 gtcatctttt tgtcatgatg catttaaaag tgataaagaa atgggtgaga tgacaacgat
       61 gtgatgaaca atcgttgtca gacctattag cgcacaatct caacagttat cgtcgctgtt
      121 gcagtaaatt gccccggggc tgggcgagct ccagtaaggc tcgcaggagc agctgaaaaa
      181 gttacactac ctgatttttc accatacact gttgatttac ccatgtctgt tggcaactca
      241 ccaccgttca ctttgatttc cttattattt ttgtcgtaaa tttttatccc cacgttagag
      301 tttgaagtta cgaccattga attgtccgta ttgggttcgg tagcataaaa agacgtactg
      361 atatcatcgg tattcagtat ccccaggttt tcacactcaa tagtgactgt tttagtaatt
      421 ttatgtgttg ttaccgcact tccaacaaca gatgaaaaat ctgcagctgg tatttcttta
      481 aaatcaaaat aaactggctg taaattattt atttcgcaac tttgcggagc tgtaatatcg
      541 ccccgaatat aaacatcagc catcggctgt gagtaatcca ccggggtgtc acggctaatt
      601 gttccataca atgtcactat ctttgtttca ggaataatca attttcctgc aaatgtcttc
      661 ttgatataaa aatatactat cgctttaccc ccactggata tacttaaagg ttcaatgcgg
      721 ttgcaattgt actttgtatt accagacggg ttgttagcat gatattcaaa aggtgcattc
      781 acatacccaa ccccaagaat atctatagca atcccaacat caaggttcgg attaagatca
      841 taataattaa tattagagtg aaccccagca taagttaatg cctggtttat ttttgacgta
      901 taatagatat aggggaattc tcctacatta ccagtatcac aatgacagcg tccaggatat
      961 gaggatccat tagttacagt ggtattaaat gtcgttccaa ccttattttc gttcgcggta
     1021 ataagatatc ccgagaaatc aagcgtacgg tgaaatacgc cattatcagg ggtacagata
     1081 cctttaggag ctgcaagtgc tacactactt aacgagagca taaacacagc aaagcaccac
     1141 tgtttgatat acttgttcat aaaaatcctt ttaatgacat gtcacaccct gttcaattaa
     1201 tccgctctca ctttctggtg tcgaaagtga ataattgaag atgcattttg ttgaggcgtt
     1261 ttctccccag gataacgtaa cctgtccttt ttctggtaga ccagaaagat aaagttcgcc
     1321 acggtcaccg acaattcctg aatctgccaa acttgcatct tgtatcgcag caatggctcc
     1381 aaaaggtacc ggtttaccat ttgcatttaa cactctgaag agaacgcgat aacccacatg
     1441 agtcacaaac tccgctttaa ccactgcacc acgcgtggga accatcgtaa cgacggtatt
     1501 atcaagttct atattcgact tcgagaaata gttatcgctg attgctaccc gattttcatc
     1561 ataagggttt agctgtgtct tgaccgtata gccgcgccag tccgtcgcag caccgcgcat
     1621 attatctatt tctaacccgg cggccccagg tgctttaaca agagcaatag tttcacctaa
     1681 ctcctgactt aacgttaccc cttcgctgtg tgccagaatt ccccctctga taccataatt
     1741 cagcctctgg gaattttggc tgtagttata gctgccgttc accgtgccat aagagccatc
     1801 ataggtcagg gacatattac cgccaacctc atcatcatga ctgttatagg actgcattaa
     1861 attccatgaa aggttatttc tttccagcgc cgtaccgctg agtccgagtt gatgtgagct
     1921 accggaatta tcgttttgcg aaacagaata ggtggcgtaa ctattacccc accagcccaa
     1981 taaaggaaca gataaagaga gcatgtagat ctgctcactg gtgttatcac tggcatggtt
     2041 gttccccgtc ccctgtctgg cagtattgga ataactccag gagacgttcc acgagagacg
     2101 cttccagcta ctgctgtagc caaattgcat taatcgttcg acatcatcgg tatgccagta
     2161 ttgttgccgg cttaatgtca ggtataaatt gccataatct ctccccaacg actgcgttag
     2221 cgtcccctcc acgcaactac gtcggttacc tgtttcccaa aagtcttcag ggctgttgcg
     2281 ccgcgatgcc cactcattga gggtatagta gccctcggtg gagtaacgat agccaaccaa
     2341 gcgcaagcta gtgtctgtgt cgtcaaatcg ttttgaatag agaaagcgat atgactgacc
     2401 cgtttctgta tcatcatggc taaaattcgc acgagcatgt gtcacatcga aagaaattgc
     2461 gcccaaagcg ccaagatctt taccgacacc cagagctgca gaaccataat tttcagcagc
     2521 ctgcatacct ccatataaag tgatcccgtg ggaaaaacca tgaagtattt gcgcctgaag
     2581 gacgtccggt gtaaatccgt cttcatcgcg caattcaccc acgctgacat caacatctgt
     2641 ctgaccttca cgtttaagaa tcgctaatga agcataaggt tgggtgaatc gttgttcgct
     2701 accgtcttct tctttgatgg ttacg
//
LOCUS       DAZSBF010000359         2721 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZSBF010000359 DAZSBF010000000
VERSION     DAZSBF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.364"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            30..2546
                     /locus_tag="ACHXWD_004256"
     CDS             30..2546
                     /locus_tag="ACHXWD_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7622959.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="ACHXWD_004257"
     CDS             2557..>2721
                     /locus_tag="ACHXWD_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7622960.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAZSBF010000360         2704 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZSBF010000360 DAZSBF010000000
VERSION     DAZSBF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.365"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..248
                     /locus_tag="ACHXWD_004258"
     CDS             <1..248
                     /locus_tag="ACHXWD_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR7622961.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(288..1496)
                     /locus_tag="ACHXWD_004259"
     CDS             complement(288..1496)
                     /locus_tag="ACHXWD_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR7622962.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1504..2005
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004260"
                     /pseudo
     CDS             1504..2005
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2023..2691)
                     /locus_tag="ACHXWD_004261"
                     /pseudo
     CDS             complement(2023..2691)
                     /locus_tag="ACHXWD_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          626 a          665 c          664 g          749 t
ORIGIN      
        1 tggctgccct gcccgtaagt tggatccaga tgctggcagg tctggcgctg ttgagtacca
       61 ttggcggcag tttgtatcag gcgctgcata atgagcgtga gcgtgagcgc gacgcggcgg
      121 tggtggcatt tctggtaacg gcaagtggat tgacgctggt cgggattggt tctgcgttct
      181 ggggattaat tgccggaggc gtttgttacg tggtgttgaa tttaatcgct gacagaaacc
      241 gatattgaca tcctccacgc cctgaaggac gtggatccct gctacgttca ggctgtcgcc
      301 tgaatcattt cggtgggttc ctgcttcaac gggcggcctg actgcaccat ctctccacag
      361 gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcac catttacatc ggcgttcgct
      421 gtatatccac atacctggca tctgaattta ctttgtgaca ggcggttctc tttcgctgta
      481 tgaccacagt acgcgcaacg ctggcttgta tacgctggcg gaacagcaag cacctgaccg
      541 ccacgccaga gctgcttgta ctcaagctgg cggtgcattt cataccagcc ctgatccagt
      601 atcgaacggt ttaaacctga ttttgcccgg acattgcgcc ccggctggct gaccgtaccc
      661 gctgctgact ttgacatgtt gctgaccttc aaatcctcaa tgactatcat tgcgtggttt
      721 ttgctgacgg ccgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa
      781 tgcagtcgct gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg
      841 cttaactgtc gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc
      901 tcaaagactg tgccatctga cagcgtggcg agtttagcca cgccagcatc cagcccgacc
      961 attgatgctg aagggtgagc cggagtggat acttcacttt ctgtctgaat actgatgtac
     1021 cacgtaccgc aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggcta
     1081 ttccggtagc gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc
     1141 ttaacgccct gcgggtagcg gaatacatca ttctgtcccc gctttttgaa tcggggaaaa
     1201 gcagcccgct tccggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc
     1261 aatggctgtg agggagaatc tttaagccat tgcgtttcag tggcattttt ccactcaacc
     1321 agccaggaag ccattttccc gtaagggatg tatttattac cggcctcatg attctcattc
     1381 tgacgtgcca gagcacgatt gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca
     1441 cactcctgtt gaccacctgg tcttaactgg aatttaaatg cctgtaatcg tttcacaggt
     1501 caaatgatac tttggtctgt gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt
     1561 ttcctgatgc atgttcacct ggtctttgtc actagatacc gacgccagat tttttgacgc
     1621 gacagaaaaa ctacgcactt acttttcaaa tgtatgtgct tattttgaag ctgaactggt
     1681 tgaaatggat ggcgaaccag atcacgtcca tttgttaatt aactaacctc ccaaactggc
     1741 gatatccagt ctggtaaaca gcctcaaagg cgtatcgggt aggttactgc gacgagatcg
     1801 accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg gctattttgc
     1861 cagtagctgc ggaggtgtgc caatatccgt catccgccaa tacattgaac aacagcaaac
     1921 acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg gttttacggc
     1981 gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt tgttaacgtc
     2041 aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt cgaaagccat
     2101 tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt tgataaccgg
     2161 tttattagtc gctgcatcaa tcagctcacc ttcaaaatag agtcgagtat ccatggtacg
     2221 gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca cttcatagaa
     2281 ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa tcagactacg
     2341 cggcccagcg gtggtaacca gtggtttacg ctgcgctatc gcttctttca tttcggtgtt
     2401 ggtatagttc aaaattttat ccagaacttt ctgccctacc taggtagaag gttttggcac
     2461 cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat caaaactcgg
     2521 gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta aatcagagta
     2581 attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac aaccagacag
     2641 agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca tgaatagttt
     2701 cctg
//
LOCUS       DAZSBF010000361         2690 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZSBF010000361 DAZSBF010000000
VERSION     DAZSBF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.366"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            44..769
                     /locus_tag="ACHXWD_004262"
     CDS             44..769
                     /locus_tag="ACHXWD_004262"
                     /inference="COORDINATES: protein motif:HMM:NF045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adhesin"
                     /protein_id="HFR7622963.1"
                     /translation="MKILNSNLITNISSTIRNHLLTSSKNIKTHPSPNMDVTSHRRLF
                     QKDGIGLSQTGKATQLVIMSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDN
                     DFTKGLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIY
                     RKEYATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKV
                     INESGVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHXWD_004263"
     CDS             complement(995..1318)
                     /gene="mobA"
                     /locus_tag="ACHXWD_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFR7622964.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="ACHXWD_004264"
     CDS             1604..1798
                     /locus_tag="ACHXWD_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFR7622965.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
     gene            2296..2457
                     /locus_tag="ACHXWD_004265"
     CDS             2296..2457
                     /locus_tag="ACHXWD_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7622966.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRL
                     EVMRLLRLN"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAZSBF010000362         2667 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZSBF010000362 DAZSBF010000000
VERSION     DAZSBF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.367"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            408..551
                     /locus_tag="ACHXWD_004266"
     CDS             408..551
                     /locus_tag="ACHXWD_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR7622967.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            564..773
                     /gene="cspE"
                     /locus_tag="ACHXWD_004267"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             564..773
                     /gene="cspE"
                     /locus_tag="ACHXWD_004267"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR7622968.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            939..1748
                     /gene="rlmA"
                     /locus_tag="ACHXWD_004268"
     CDS             939..1748
                     /gene="rlmA"
                     /locus_tag="ACHXWD_004268"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR7622969.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1745..2311)
                     /gene="mntP"
                     /locus_tag="ACHXWD_004269"
     CDS             complement(1745..2311)
                     /gene="mntP"
                     /locus_tag="ACHXWD_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR7622970.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          722 a          608 c          629 g          708 t
ORIGIN      
        1 aatttttctt attttaaatt ttcaatccct ttggggattg acttctcttt agggtaattg
       61 atagccgtta accgactgtt ttatgagaaa aagtgatata actttttatt cattgcatag
      121 caaaaaatgt gatattgcac gcactatgta ataacttctc ccactggcct ggaacaactg
      181 aacttattga actatgttag aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt
      241 taagcacaat gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga
      301 cgtatcgaga tttgtgcgtc tgatcgagac atgtttaaaa atggcttgcc ataattaacg
      361 ttgtatgtga taacagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt
      421 tcagtaaaga agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt
      481 atattggtgc ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga
      541 aaacaatcta aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg
      601 agtctaaagg ttttggcttc attactccgg ctgatggcag caaagacgtg ttcgtacact
      661 tctccgctat ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg
      721 aaattcagga cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc
      781 cactgatctg aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc
      841 tttttactat gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc
      901 gttacacttg cggcctttag tatcctgccg gagttgtcat gtctttttcc tgtccacttt
      961 gccatcagcc tctttcgcgt gaaaaaaaca gctatatctg tccccagcga catcagtttg
     1021 atatggcgaa agaagggtat gtcaatctgc tgtccgttca gcataaacgg tctcgtgatc
     1081 cgggcgacag cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc
     1141 agccgctgcg tgatgcaatt gtcgcccaac tgagggaacg gcttgatgat aaggccacgg
     1201 cggtgctgga tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc
     1261 ccgaaatcac cacgtttggt ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac
     1321 gctatccgca ggtcactttt tgtgtcgctt ccagccaccg tttaccgttt tccgatacca
     1381 gtatggacgc cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag
     1441 tgaagcccgg cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga
     1501 aggggctgat ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt
     1561 ttacattaca gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg
     1621 tcgcattatt gcagatgacg ccgtttacct ggcgtgcgaa gccagaagtc tggcaaacac
     1681 tggcagcaaa agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt
     1741 cttattaacc gtggaagtgc gtccagagga tctggacgcc gatgccgatc agtaccagcc
     1801 cgccgagaat ttctgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc
     1861 ctaatgttga cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga
     1921 cctgcaggaa agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg
     1981 tggttaccag tagccagaaa ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc
     2041 cacgaaaacc ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc
     2101 agtggttcca ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg
     2161 gcgtcagggt ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaatttcg
     2221 gtttatggag ggtggcacct ttaccgattg atgcagcaaa tgcatccatc gacataccaa
     2281 acgcaagaag aacagtagca gtgatattca tgacaatgtc ctgaccgggg tatccataaa
     2341 acacatcatc gcccccagca aacatgcata catgaacaac aagccgtgat ggttttcgcg
     2401 ttcatgcagg catagcgatt gatgtgcata tggtctcgcc tgactgaaag tattcagtcc
     2461 gtacgtgcca cgttttgcaa cgagtatgtt gacacgtaca tttccggaat ctggaaatcg
     2521 gctactcccc aaatgagggc gcaaccttaa catattttga gaatataaaa caactgttgg
     2581 aatatattat ttcattgctt tattgataac gattttcatt tggatttata tatcaatgaa
     2641 acgttaaaat aaataacaaa taaggga
//
LOCUS       DAZSBF010000363         2644 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZSBF010000363 DAZSBF010000000
VERSION     DAZSBF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.368"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            12..107
                     /locus_tag="ACHXWD_004270"
                     /pseudo
     CDS             12..107
                     /locus_tag="ACHXWD_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="ACHXWD_004271"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="ACHXWD_004271"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR7622971.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="ACHXWD_004272"
     CDS             complement(1439..2392)
                     /locus_tag="ACHXWD_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR7622972.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAZSBF010000364         2626 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZSBF010000364 DAZSBF010000000
VERSION     DAZSBF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.369"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..519
                     /gene="virA"
                     /locus_tag="ACHXWD_004273"
     CDS             <1..519
                     /gene="virA"
                     /locus_tag="ACHXWD_004273"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR7622973.1"
                     /translation="KELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFIS
                     ALTSAFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKN
                     DTAISASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPES
                     RQVSFAIISPDV"
     gene            698..1385
                     /locus_tag="ACHXWD_004274"
                     /pseudo
     CDS             698..1385
                     /locus_tag="ACHXWD_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    978..1094
                     /locus_tag="ACHXWD_004274"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1387..1753
                     /locus_tag="ACHXWD_004275"
                     /pseudo
     CDS             1387..1753
                     /locus_tag="ACHXWD_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1756..2154
                     /locus_tag="ACHXWD_004276"
                     /pseudo
     CDS             1756..2154
                     /locus_tag="ACHXWD_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
BASE COUNT          713 a          635 c          688 g          590 t
ORIGIN      
        1 aaagagcttc aaatgctaag ttacaaccaa gtaaataaaa taatcaattc aaacttcccc
       61 caacaagacc tatgttttca aacagaaaaa cttttgttta cttcactctt tcaagaccct
      121 gcatttatat ctgcactaac atctgcattc tggcaatctc ttcacatcac gtcttcctct
      181 gtggaacaca tatatgccca aattatgtcc gaaaacatag aaaacaggct taatttcatg
      241 cctgaacaac gagttattaa caattgtgga catattataa aaatcaacgc agttgtccct
      301 aaaaacgaca ctgcaatctc tgcctctggg gggagggctt acgaagttag ctcatcaata
      361 ttaccatcgc acataacatg taatggtgtt ggtattaata aaatagaaac aagctatctc
      421 gttcatgctg gaacacttcc aagcagtgaa gggcttcgca atgctatccc tccagaaagt
      481 cgtcaagtat catttgccat catatctcct gatgtttaag ccaatattat attggctgcc
      541 agactgtaaa aaaacgttct tcagaacagc tctataaact attaaaccaa agctaaaagg
      601 atggggacca atagaatctc agcccatcct ttttatatca ttatgaaccg ccccggaaat
      661 cctggagact aaactccctg agaaagaggt aaacaggatg actaaaaata cacgtttttc
      721 ccctgaagtc cgtcaacggg cagttcgtat ggttctggaa agtcagggcg aatatgactc
      781 acaatgggca gcgatttgtt ccattgcccc aaagattggc tgtatactgg agactctgcg
      841 tgtctgggta cgccagcatg agcgggatac cgggggcggt gaggtgggct caccaccgct
      901 gaacgtcagc gtctgaaaga gccggaacgt gaaaatcgtg aactgcgccg cagtaacaat
      961 atccttcgcc tggcttccgc ttattttgca aaggcggagt tcgaccgcct ctggaaaaaa
     1021 taatgccact gctggataag ctgcgtgagc agtacggggt cggatcggta tgcagtgaac
     1081 tgcatattgc cccgtcaacg tattaccact gtcagcaaca gcgacatcat catgataaac
     1141 gcagtgcccg tgcgcagcgc gatgactggc tgaagaaaga gatactgcgc gtatacgatg
     1201 agaatcatca ggtatacgct gtgcgtaaag tctggcatca gttgttacgg gaaggtatca
     1261 gagtggccag atgcactgtg gcacgtctca tggcggttat ggggcttgcc ggtgttctcc
     1321 ggggtaaaaa ggtccacact accgtcagcc ggaaagccgt tgccgcaggc gaccgcgtaa
     1381 accgtcatca aggcaatatg ccgcgtactc cgggtggccc gcagcggctg gtatacgtgg
     1441 tgtcagcggc ggacaaggat aaacacacgt cagcagttcc gtcagcactg cgacagcgtt
     1501 gtccgcaagg cttttaccca gtccagcgtt acggtgcccc acgcctgacg gatgaactgt
     1561 gtgctctggt taccacttta acgtaaaaac cgtggcagcc agtctgcgcc gtcaggaact
     1621 gagcgcaaaa gcctcccaga agttcagccc gatcagttac cgtgcacacg gcctgcctgt
     1681 atcagaaaat ctgctgacgc aggattttta cgccagtggc ccgaaccaga agtgggcggg
     1741 agacatcacg tactattaca gctcaccgac tgcggggaaa cacggggcgc cgggttattg
     1801 aaggtgacct gtccagctac ttcgacacag tacatcaccg gctgctgatg aaagccgtct
     1861 gccgcaggat cagtgatgca cgttttatga gactgctgtg gaaaacgccg tgttgatgtt
     1921 ggtctcttcc gggcggtcag tgaaagtatg ccacagggcg gtgtgatctc gccgttgctg
     1981 tcgaacatca tactgaatga gttcgaccag tatctgaata aacgctacct gagtggaaaa
     2041 gccagaaagg atcgctggta ctggaatcac agtatccagc gaggccgaag tactgctgtc
     2101 aaagaaaact ggcaatggaa acctgccgtt gcttactgtt gctatgccga tgactgtgtg
     2161 ccacgaaggc gcgtcctggg gacgtgagtc cctgtgcagc catgcggcac aagagatgag
     2221 ggaaggcccc tcggtgtcgg cgtgcaggcg caggcaccaa accatccact gctgctgcat
     2281 ttaagagatg tggtggtgag cgagatggga aaaggtcgga cgtgagccga tcaggtatgg
     2341 ttcgaagaga cgaacgaaag tgaaccgctg atgatgtgtc gaaagcatat agatgatgtc
     2401 aaaacaatgg tggagtcgtt cctgtgggat atgtttactg cctgcgcggc atccggcata
     2461 aaagcggcgt gaagtcgatt caggctcttg tggggaacgt ggacctgtca ccccgatgtg
     2521 aagggagaag cgcaagtgga agccccatga gcgccagagt accaatgcgg ggtacaggga
     2581 cggagcacca agcgattgcg cagtaaacat gcttgacaac ttaatt
//
LOCUS       DAZSBF010000365         2579 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZSBF010000365 DAZSBF010000000
VERSION     DAZSBF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.370"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(253..489)
                     /locus_tag="ACHXWD_004277"
     CDS             complement(253..489)
                     /locus_tag="ACHXWD_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622974.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARA
                     QIELQRYADRQRALREDERQQSDFAWSRPRAPRR"
     gene            1557..1712
                     /locus_tag="ACHXWD_004278"
                     /pseudo
     CDS             1557..1712
                     /locus_tag="ACHXWD_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1806..2450
                     /locus_tag="ACHXWD_004279"
     CDS             1806..2450
                     /locus_tag="ACHXWD_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="HFR7622975.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGE
                     FSTFDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
BASE COUNT          648 a          649 c          655 g          627 t
ORIGIN      
        1 aaaaaaaaag ccccctaagg gctttttttt gtacctgacg gtcagaccga ttaaacataa
       61 catcccttgc ccgcaccccc gcaggcgccc tcagcgcaat cctgctgcat cagggccgtt
      121 tccagcacct ttcctcacca gtatcccgga aaaacgcccc tgatgatttc tgcgcgactg
      181 catggctatg cacgcaggtg aatggcaggt gatttcgcag cgtcagcacc actaaaccgc
      241 caaaagccaa aatcagcggc gcggcgcgcg cggacgtgac caggcaaaat cggactgctg
      301 ccgctcatcc tcacgtaatg cacgctggcg gtcggcgtaa cgctgcaact caatttgcgc
      361 acgcgctctg ttcaggacga tgtgccggtt aggctgattt agctttttac ctgacgctct
      421 ttcagctgcg gcaacagcat catcaagaaa ttttttctct gcaggcgtca agtttgtcag
      481 tctggccata acgttctcca gtcagaataa atcagcacag tctgccagac acagtagaaa
      541 cgatgaagcg ccagcgacgg tgaaaggcag tcagaaacgg tgaacgggag cggattttaa
      601 tccggtgccg tgaggcgttg gacaagccgc aggcgcgtca ttagctttta gccggtttcg
      661 gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct gactatgttg
      721 gcacggatgt ggaaatcagt gaagtgcttc atgtagcagg agaaaaaagg cggcaccagc
      781 gcgtcagcag gatatgtagt acaggatata ttccgcttcc tcgctcactg actcgctgcg
      841 ctcggtcgtt cggctgcggc gagcggtgcc ggcttacagg cggggcggaa atttcctggg
      901 agataccagg aaggagctta acagggaaga gataaggccg cggcgaagcc gtttttccat
      961 aggctccgcc cccctgacaa acatcatgaa atctgacgct caaatcagtg gtggcgaaac
     1021 ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg ctcccccgca
     1081 ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta tctcattcca
     1141 cgcctgacac tcagttccgc gaaggcagtt cgctcctgga tggactgtat gcacgaaccc
     1201 cccgttcagt ccgactgctg cgccttatcc ggtaactatc atcttgagtc caacccggaa
     1261 agacacgact tatcgccact ggcagcagcc actggtaact gatttagagg agttagtctt
     1321 gaagttatgc gcctgttaag gctaaactga aagaacagat ttggttactg cgctcctcgt
     1381 aagccagtta ccacggttca aagagttgct gctctgagaa ccttcgaaaa accgccttgc
     1441 agggcggttt tttcgtttac agagcaagag attacgacgc cacagaaagg atctcaagaa
     1501 gatccttcac cgtaatggaa atctatccaa tccgagcaca taggatacac atcgttataa
     1561 cgctggatct gcgtgaattc cagcagcagc aagagaaaga ctttctacaa accagcttac
     1621 agcaggctaa atttaaccag aaaaaagccg ctgaattact gggtctgact tatcatcagc
     1681 tacgtgcctt gctgaagaaa caccagatct aatacgcatt tgcaggtgtc atactggcgg
     1741 gtttgacgca taacgtcata aggtttacca tggcgtcatt actgtataaa aacacaggca
     1801 tagatatgac tctggcatta gttggcgaaa aaattgacag aaatcgcttc accggtgaga
     1861 aagttgaaaa tagtacattt tttaactgcg atttttcagg tgccgacctg agcggcactg
     1921 aatttatcgg ctgccagttc tatgatcgcg aaagtcagaa agggtgcaat tttagtcgcg
     1981 caatgctgaa agatgccatt ttcaaaagct gtgatttatc aatggcagat ttccgcaacg
     2041 tcagtgcctt gggcattgaa attcgccact gccgcgcaca aggcgcagat ttccgcggtg
     2101 caagctttat gaatatgatc accacgcgca cctggttttg cagcgcatat atcactaata
     2161 ctaatctaag ctacgccaat ttttcgaaag tcgtgttgga aaagtgtgag ctgtgggaaa
     2221 accgctggat ggggactcag gtactgggtg cgacgttcag tggttcagat ctctccggcg
     2281 gcgagttttc gactttcgac tggcgagcag caaacttcac acattgcgat ctgaccaatt
     2341 cggagttagg tgacttagat attcggggtg ttgatttaca aggcgttaag ttagacagct
     2401 accaggcatc gttgctcatg gagcggcttg gcatcgctgt gattggttag ttcagcgttt
     2461 acaccgggag atgcgtttga cgatttactt ttacccgcga tcatcgcgtt tattgagttt
     2521 attatttatc tatgcgattc cgatgaaaaa ttcgcacgaa aatatggcta actcatagc
//
LOCUS       DAZSBF010000366         2556 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZSBF010000366 DAZSBF010000000
VERSION     DAZSBF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.371"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHXWD_004280"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHXWD_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR7622976.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHXWD_004281"
     CDS             complement(390..1178)
                     /locus_tag="ACHXWD_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR7622977.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHXWD_004282"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHXWD_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR7622978.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHXWD_004283"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHXWD_004283"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR7622979.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHXWD_004284"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHXWD_004284"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR7622980.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZSBF010000367         2501 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZSBF010000367 DAZSBF010000000
VERSION     DAZSBF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.372"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHXWD_004285"
     CDS             complement(25..315)
                     /locus_tag="ACHXWD_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR7622981.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHXWD_004286"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHXWD_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR7622982.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHXWD_004287"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHXWD_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR7622983.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHXWD_004288"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHXWD_004288"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2501)
                     /locus_tag="ACHXWD_004289"
     CDS             complement(2171..>2501)
                     /locus_tag="ACHXWD_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR7622984.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTA
                     QTHMAWGDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGT
                     IRAFDVT"
BASE COUNT          677 a          672 c          559 g          593 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgaccattc cattcaaacg
     2461 tgaacggctt cgcctcctgt tctgtgcgcc atgcttcaat t
//
LOCUS       DAZSBF010000368         2486 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZSBF010000368 DAZSBF010000000
VERSION     DAZSBF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.373"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(164..283)
                     /locus_tag="ACHXWD_004290"
                     /pseudo
     CDS             complement(164..283)
                     /locus_tag="ACHXWD_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            527..899
                     /locus_tag="ACHXWD_004291"
                     /pseudo
     CDS             527..899
                     /locus_tag="ACHXWD_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1461..2408)
                     /gene="icsP"
                     /locus_tag="ACHXWD_004292"
     CDS             complement(1461..2408)
                     /gene="icsP"
                     /locus_tag="ACHXWD_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFR7622985.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIDYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
BASE COUNT          713 a          539 c          510 g          724 t
ORIGIN      
        1 atgatttaaa aaaacggaac aaaaatgcaa ttatattctg gcattgttta atgctgtttg
       61 tcttttttat tcattatttc aatgaaagaa tttcaactac aaagttaatt gatcctacct
      121 acataatata gacacagtac ctaaagtacc gtggagatca tccggggaag ccccgggtct
      181 gataccagtg caggtagacc gctatcttgt tccggtaatg ttctgccgtt ctttacggca
      241 tcacctctag cagaaacatc gcgtaactgc gccaagtatg atgaacttct cttaccgctg
      301 cgatctggtt caacaatatg cagatggtac acaacctctt taaccagccc atccggatta
      361 ttcagagccg cttccgcaat ccggaataaa agtgctgttt taccgtgaac cctctgaaaa
      421 tcagcgagca gtgttttaac cagccgacgt tcagagcgta ctgacagacg gtggacaatt
      481 tgaataaaca gttcaatcag accgtagatg ctctcccgtt ggcgacatgc tgggagcggt
      541 ggaacaccgc ttcggcaacg agcttccggc gtctccagtg aagtgactga cggataatgg
      601 ttcatgctac cgggctaatg aaacacgcca gttcacccgg atgttgggac tttgttgaac
      661 cgaagaacac ggcggtgcgg agtccggaga gtaacagaat agcaaagagc ttcgtgaaaa
      721 caataaagtg tgactacatc agtatcatgc ccaaacctga cgggttaacg gcagcaaaga
      781 accttgcaga ggcattcgag cattataacg aatagcatcc gcatagtgcg ctggattatc
      841 gctcgccacg ggaatatctg cggcagcggg ccaatgataa caggtgtctg gaaatatagg
      901 ggcaaagcca cccacgaacg atccagcata taatcaagaa attagatttc atttaacata
      961 agatagatta tgtgcaccaa gaggcgtttc cttaatttct gaatttacag gctcttttgc
     1021 tctctttgct atgctgtgga tttggaaaga tagagtgctg gctgattatt taggtatttt
     1081 catcagcatg tccctgatag cactgttcca tcagcgcttt caggcgataa tagtagacct
     1141 tgggtagcct ctgcccatgt gtaacttgat accccgagaa cagccacgat gatctaagcg
     1201 gttcttattt ctccggtctc agggtcggta atgccaacag taagtccaca gtaatcaacg
     1261 aagagttttt tgaaccgccc cggaaatcct ggagactaaa cgccctgaga aagaggtaaa
     1321 cagaatgact aaaaatacac gtttttcccc cgaagtccgt caacgggcag ttcgtatggt
     1381 tctggaaagt cagggcgaat atgactcaca atgggcggca atttttagcc atgaaaacgt
     1441 aatcaactcg gtgaaccaat tcaaaaaata tactttatac ctgcggaagt gagaaaacca
     1501 atatattcaa tacttgcact atttttaatg gtgccagata tattggtact ttgctcataa
     1561 agagatgtat cacctttttt attagaaatg taatttctgg agccttctat ataaaatttt
     1621 gcactggggg tgacatagta tccaatattc gcagcaacag agcagtaatt ttggtttttt
     1681 atttcatctt taaaaattgt ctcagtctga tagtgtttat ctgtatcaga tgaacgaacc
     1741 caaccactat atttcagttc tgctccaaac tcaaaattct catggcggta attagcagtt
     1801 aatccaatat aaggtatttt gaagagctgt ttgtaatcta ttgttctttc accactagga
     1861 tgtgcccctt ttttattcct gttccccccg ttttcactat aaatataact ccctcccatt
     1921 gcattaaaac tgtaacgact ctcttggtaa cctgctatta gtccaagtcg ataatccaaa
     1981 ttatttaata accaaccttt aagattcagc tcgaattcgt tagcatcacg gagatgcgta
     2041 ttgggatgcc agctctggtc ggtccatacc tgaggggtat tggaattgtt ccagtcttta
     2101 tcaaccatgc ttgctttctg gttacctaaa gtagtccaac cggaaactcc gaaagacact
     2161 tttggaatta gcttccaatc gatgccacct ctaacaatag tcgcattact gtatttccag
     2221 tccagttgac taatttttct ccccccttcc ttgggatgat aaacgcgttc ttttgttttg
     2281 ccactcagag atcctagact aatatccgta ctgatgttgt ccggtatgaa aagtggatag
     2341 ttagtggtag catgtgtagt aaagatcgct ggtacacaaa gtgcaagtac aaggaatttt
     2401 aatttcatcg agaactctac ttttttggtt gaaatgtcca tgatcttctt actttataag
     2461 ataaacgtca atgccaagca ttattt
//
LOCUS       DAZSBF010000369         2472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZSBF010000369 DAZSBF010000000
VERSION     DAZSBF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.374"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..713)
                     /locus_tag="ACHXWD_004293"
                     /pseudo
     CDS             complement(1..713)
                     /locus_tag="ACHXWD_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(766..1362)
                     /locus_tag="ACHXWD_004294"
     CDS             complement(766..1362)
                     /locus_tag="ACHXWD_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR7622986.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="ACHXWD_004295"
     CDS             complement(1389..1991)
                     /gene="yadL"
                     /locus_tag="ACHXWD_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR7622987.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2006..>2472)
                     /locus_tag="ACHXWD_004296"
     CDS             complement(2006..>2472)
                     /locus_tag="ACHXWD_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7622988.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          679 a          537 c          496 g          760 t
ORIGIN      
        1 tggcctgcgc cataggcttt aaatgaatac agataatttg atgagctgga taatttgatc
       61 tgttgagtta ctgttggatc aaagtttgta tctgtataga attcaattgt aagggtctga
      121 taaataccac ctacagccca gtatttacgg gtgctattcg cattattgca actccaatat
      181 aacacgtctt cgcttggatt ataccacgaa aaatattgtt ctgcagagtc accaatgtat
      241 atcccaggtg aactaacgtt agttacggta ccgtaagctg accacatgtt tgaaattaat
      301 aatgtgtaat acaaaccagg aactgatgta ttaaataatt tatgggagcc atacatttta
      361 cctgagtcaa ccattgcatt ttcaatacgc attgcgctac cacttgtgct atcacttctg
      421 ttgcaataaa gaaaaccacc accagaagcg gtatctaact gactctactg taattttaca
      481 cctgtaggtc caagctgcgt cggtttgtag aaaactaaat tatttgtact tgttgcatat
      541 ctgaatatga cagtagcatc gccattcggc cccgtttctc tccatttttg atctgacgga
      601 ccaacgccgt aactattccc aacaatatat cctacgttat catcagtgcc agcactcgcg
      661 gtataacaac tcaagcacag aattaaaaaa aaatgtctga agaatttctt cattatcact
      721 ccaccatcta ctgctgtaac agtcgcagtt atttgttttg atctattatt cgtagtacac
      781 atcaactaat atatgactca ttacttggcc agatatgaca tccagcgcgg gatcgatttt
      841 ttgcattctg gcataaaatt tccatgaaga attatgcagg gcagaagtca ttactggata
      901 cacggaacga gaatttcctg ctgaactaat aacattgaat atattggtcg ggtcctgaat
      961 ggaaaagaga acgacaccga catttttagc cccggttgcg acttcattgg caaatatttg
     1021 ccttgaacct gcagccatga caccgctaag ggctctaaaa tcaatatgta actgattaag
     1081 cacatcgcca gttccctgta atgcacagtc tgatactgtg acagtgaact cttttccacc
     1141 tggttgatag ctttcaggag taacattatc tgaaaaatat cccaccccaa cgacgccaag
     1201 atcaatatta ccctggttac tgattccgct tttgcaagtg ctgtttttta cattggcaac
     1261 aaggtcaata tcctgcccag cgatggctgg ataggtaatg ggaaactgta gtgacagaaa
     1321 cagaattatt ctcttcatca gcttacgttg agtgggatgc atatatagtt cctggatctg
     1381 tcagtctgtt agttataggt tactgtaaat gtggcaggtg cagaaaattt acccgcagtg
     1441 acattgctta cggttttatt tttttcgacg accagtcgag cactcatcgg ataataaaca
     1501 accgcgttgg ctgcattgga gatagtgacc tcttgagatg ctggtgtcac acaactaaat
     1561 tgtgttgcgc tccctgttgc cggagtttcg gtgctccaga cttcaacggc gacggcactt
     1621 gctttatctg cggctgtgga tccatttgcg agccccgcac cgtcattagc agtaccttcg
     1681 catgtggctc gcttggttaa ttttatttgt gcttttttat tggggatacc cgcacagtct
     1741 ttgaatttga gtttgaatgt tttaactttg gtcttggcat tgatttcaga aatatataca
     1801 tcgccaaagt caacaacaga aatgtcctga tcagagtcat ttactaatgt agcggtacaa
     1861 gtacctattt ctacggtagt aatcacggtc agcccaatgc tgtctgttgc cgcataagag
     1921 ggtacgctga acaatgaggc agccaaaacg acactgctgg ataatccata acgtaaattt
     1981 ttaaatgtca tcttaattat ccttcttatt gatagatgaa attaaacgtt gccagtgcac
     2041 gaaaattccc cggtacgcct tgccctgcat ctgtttcacg tagcgcaacg gtcatttcaa
     2101 gacccttatc gggctgcatc tcgtctgtgc tccagcgtat cttttccgca ctgttaggtt
     2161 taaggaaagt ggcatcatca gtagtccgtt ttttgaaacc cataccgata ttacttgtcg
     2221 tcgaagatga atcaccagac tgcggtataa taagcttagg tgagcttgat gatgcatttc
     2281 cggtcagagt ggtatcaatc caactgatgc cactgctgca cccactagca accagtttaa
     2341 aatcagcctg agattctgtc gttttattcg cgatcttatc cagacccatc ttagggattc
     2401 tcaatgtgta gttattattg ccatcattcg tgacgttatt accagtaagt gtgatgttac
     2461 aggttgtcgc tt
//
LOCUS       DAZSBF010000370         2451 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZSBF010000370 DAZSBF010000000
VERSION     DAZSBF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.375"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(356..1135)
                     /gene="ipaJ"
                     /locus_tag="ACHXWD_004297"
     CDS             complement(356..1135)
                     /gene="ipaJ"
                     /locus_tag="ACHXWD_004297"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFR7622989.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1332..2141)
                     /locus_tag="ACHXWD_004298"
                     /pseudo
     CDS             complement(1332..2141)
                     /locus_tag="ACHXWD_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          737 a          617 c          377 g          720 t
ORIGIN      
        1 ttataatatc catacccctc atagggctta cagtttaaat tgcgggtata aaatacatct
       61 attttcaaat aaaataaagc atactccaat tacaacgagt gactaaaata aaaatatcat
      121 ccaagtcaat cactgtttgc tctgcaaaat atgtcagcct tctcaaactt accagcactt
      181 aaatatttaa gcacacaatc attccttgag ggagttcact tatttcaaga gaaacatttc
      241 gtcttcccac acaactaatc cgcccaatat agctaaattt gtactcagta atatcatttg
      301 ttatgccccc cgcccccaaa atattcaagc tccttcacag ataaaaaacg acaatttaca
      361 aagcctcatt agttataact atggaaatac ctattttagt atatcctata aagttagaat
      421 tacttcttct tgcacctaat tccatagttg aaaaacaaga ataattttct cctacagctg
      481 gatccatata gcttccatca ggtctacaca acacccagtg gaggccgaca ggaactccaa
      541 taaaggatac agcaacagct tccaaaactc tttggttaga agataacaca tctcgttgat
      601 gtactatatt acatcccatg ccaattaata agtcccttgc atcgggataa ataaaactaa
      661 ttacttgcga aaaaatgtta ctttcttcaa cgacataagc attcaatcca agaagcctcg
      721 tagccatgac tatcttatca ggcatagaat aaccggcatc cgcgatatta tctttgtgta
      781 ttctcggatt ataattacca ctagttatta agtataaatc ccgctcacaa cgattatcca
      841 aatcaagaga actcttcctt gtcatttcgg acatagaccc tttgtattgt ggtattttat
      901 caacccctaa ttcctttgca gcacagagta aactggcagc tccacaagaa ttgtcataca
      961 tctgcttcct cgccaaaaaa caaccttgtt cgtttactct attctcattt accctcacat
     1021 cagtctgatg agatatcata tattctcttg gtatagcccc ctgcaataaa accccatata
     1081 acataacacc tgtatgaata catccccgtt tgcaaggctt ccgttgttcc gacatcgacg
     1141 agtaattgca gcacagtatt gcatccagca atgcgaacta ctatgagaat tagctgaccg
     1201 ggtccttatt ccccgaacga actttaaatc caaaacctca tactcttttc cgtcgtcatc
     1261 acgtccgtca ccataacaac ctttcagcat ccaccctttt attaagagtt aatgtccatc
     1321 agcatcagca attaataccg cctgatactg acatattttt gtcgtattta cgtccataat
     1381 cggttattgt gattcaggtc acaccagctt atttgctatt gttacaataa ccaccttggt
     1441 tgagagctgt tttttcaact ttgttatcga ctcccccaac tccttggtca gtaaggctgc
     1501 cgaccgagca cagtgaatag atagtatccg caggtaccac catgcttact taatactcag
     1561 aaggcgaagt gctctccaat taactctgcc gcactatgct tataagctgc ttttcaatgc
     1621 cgtctatcaa catgtccagt tcattcaatc tgttgtactg gtaatccaac gcagtaatga
     1681 tatatgcagg tattctttca tccactccag taacgtttca tgcaaagcat taatttacgc
     1741 gctacggaac ttcaccagtt gactgagcaa acgatgtggg accagtactg cctgcagttc
     1801 ttctattgtt acagcgattt ctttaccaaa cagctgaacc accatcaaga taaagatagc
     1861 ctgaacattc atcgcaccat tttgttacca atggcaaaag atttaacgaa ccgtgcctgc
     1921 atcaggcgga ctttttatcc ctgctttgtt agctccctaa ccttgtacag agagcctcca
     1981 taagctccca tatcaagcag acatggttcg cagttactga catacaccag aaaactctga
     2041 cagcagactc acttatcctc cacttcactc atatccttat tggtgagatg aacctgaatg
     2101 acatgttttg cgacataaac gccaaccagt gtatatttca cctgtagaga ttctacaatc
     2161 tgtagcgtta tgcatctaat gttaggctct atgatgtcag aaatctgcga gactctacac
     2221 cttctgcatt tacctttatt tatcaccatg ctcctcagtt aacggagaac gtgttcattg
     2281 cattttgtca cttacatttc gcctttggta ccggggggat ataggttcca acgttccgca
     2341 tgagaacctg aaccggcttc acgccacctt gataccagat gccgcacagg cagtcaacaa
     2401 gtgtcacgaa cggtgcaata gtgatccaca cccaacgcct gaaatcagat c
//
LOCUS       DAZSBF010000371         2440 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZSBF010000371 DAZSBF010000000
VERSION     DAZSBF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.376"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..258)
                     /locus_tag="ACHXWD_004299"
     CDS             complement(<1..258)
                     /locus_tag="ACHXWD_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7622990.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYG"
     gene            523..978
                     /locus_tag="ACHXWD_004300"
     CDS             523..978
                     /locus_tag="ACHXWD_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR7622991.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            978..1148
                     /locus_tag="ACHXWD_004301"
     CDS             978..1148
                     /locus_tag="ACHXWD_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR7622992.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1141..1431
                     /locus_tag="ACHXWD_004302"
     CDS             1141..1431
                     /locus_tag="ACHXWD_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR7622993.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1428..1790
                     /gene="rusA"
                     /locus_tag="ACHXWD_004303"
     CDS             1428..1790
                     /gene="rusA"
                     /locus_tag="ACHXWD_004303"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR7622994.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1787..1927
                     /locus_tag="ACHXWD_004304"
     CDS             1787..1927
                     /locus_tag="ACHXWD_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR7622995.1"
                     /translation="MMFEFYMAEFLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            2013..2396
                     /gene="quuD"
                     /locus_tag="ACHXWD_004305"
     CDS             2013..2396
                     /gene="quuD"
                     /locus_tag="ACHXWD_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR7622996.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
BASE COUNT          647 a          550 c          604 g          639 t
ORIGIN      
        1 tccgtaaaaa ttcacatttg atagatcacc accaaaaacc ctctgcccac tcgaggtaat
       61 tgcctcacct aatttagtaa aattaccagc aatagatgat tttctttttc cacttctatt
      121 caaagtggct ttaattacac caccaccatc cttagagtta aataattgaa tgtccactcc
      181 agcagttggt tgtatatatt ttgaaagctc tttcttgaac ttgttaatat atttatcttt
      241 gttttctttg ttggtcattg gttagatcca tagttctggc tgtgattagg tgaaagtata
      301 caaggttttt aaagcgaaga tcactgtttt ggcttcaaga tagttttgat ttatcgtaat
      361 caatccgcca taatcctctc atcggagcct gaacaactcc ggtgacttct gcgcatttaa
      421 ggggacttaa atgcgaccac aatctgaact catcaccttg tcacagatgc agaaatgcac
      481 ctgcgatttt ctgcattctg cggtttccgt tagggaggcc gtatgactct gccagtagac
      541 ggcatcaaac tccatcgcgg taacttcgcg gccatcggcc agcagattca gccattgctg
      601 gatgccgggc aatgcttacg cctgcaggtt aagccgtggc gcgagaagcg cagcctgtcg
      661 cagaacgcgc tcagccacat gtggtacacg gagatcagcg aatatctcat cgcccgcggc
      721 aagaccttcg ctacgcctga gtgggtcaaa gacgcgatga agcacaccta tctcggctac
      781 gaaagcaaag accgtgtaga tgtcgtgtcc ggcgaggtca ccaccgtcca atccctccgc
      841 catacgtcag agctggaaac cggcgagatg tacatcttcc tgtgcaaagt cgaagcctgg
      901 gcgatgaata tcggctgcca cctgacaatt ccgcagagct gtgaatacca gcagctgcgc
      961 gataagcagg aggcgtaatg gctacaccgc ttattcgtgt catgaacggg cacatctaca
     1021 gagtatcaaa tcgtcgtaag cgtaagcctg agctgaagcc atccgaaata ccaacactgc
     1081 tcggatatac cgctagcctg gttgataaaa aatggttgcg actggcagca aggaggaatc
     1141 atggctgatt tgagaaaagc agcgcgtggt cgggaatgcc aggtaagaat ccctggcgta
     1201 tgtaatggca atcctgaaac gtcagtactg gcacatatcc ggctggctgg attgtgcggt
     1261 accggtatca aaccgccaga cctgattgcc accattgcat gttctgcctg tcacgacgaa
     1321 atcgaccgcc gcacatattt tgtcgatgct gagtatgcaa aagaatgcgc gctggaaggt
     1381 atggcgagaa cgcaggttat ctggctgaaa gagggggtta ttaaggcgtg aatacctaca
     1441 gcatcacatt accctggcct ccgagcaata accgctatta ccgccataat cgcgggcgca
     1501 cacacatcag cgcagaaggg caggcatacc gcgataacgt cacccgaatc attaaaaacg
     1561 caatgctgga tatcggcctg gctatgccag tgaaaatccg tattgagtgc cacatgccgg
     1621 atcgccgtcg ccgtgacctg gataatctgc aaaaagccgc ttttgacgca ctcaccaaag
     1681 caggtttctg gctggatgat gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta
     1741 ccaaaggtgg gaggctggaa ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt
     1801 ttatatggca gaatttcttc gccaccgctg ggggcatctg cgcttatatc gtttccccgg
     1861 ttctgttttg accgattacc gaatactgaa gaattacgcc aaaaccctga caggagcagg
     1921 agtatgaagt cagagataac aatcaactaa tactgttttg ttgatttttg cttgtaattg
     1981 gcgttctggt ctgatttttg tggagtaagt tgatgcgtga tattcagatg gttcttgagc
     2041 gttggggagc gtgggcggct aataatcatg aagatgtgac ctggtcgtcc attgccgccg
     2101 gttttaaggg attaattact tcaaaagtaa aatctcgccc gcaatgttgt gacgatgacg
     2161 cgatgattat ttgcgggtgc atggcccgtc tgaaaaagaa caacagcgat ttgcacgatt
     2221 tattagtaga ttattatgta gtcggtatga cattcatgtc actggcaggt aagcattgct
     2281 gctctgatgg ttatatcggg aaaaggttac agaaggctga gggcataatt gaagggatgt
     2341 taatggcatt agatatccgg ttagagatgg atatcgttgt taataactct aattaatatg
     2401 ccaattgttt actaaaaatt attaaaaatg gggcgttgca
//
LOCUS       DAZSBF010000372         2437 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZSBF010000372 DAZSBF010000000
VERSION     DAZSBF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.377"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            221..337
                     /locus_tag="ACHXWD_004306"
                     /pseudo
     CDS             221..337
                     /locus_tag="ACHXWD_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(442..518)
                     /locus_tag="ACHXWD_004307"
     tRNA            complement(442..518)
                     /locus_tag="ACHXWD_004307"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(532..608)
                     /locus_tag="ACHXWD_004308"
     tRNA            complement(532..608)
                     /locus_tag="ACHXWD_004308"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Arg,seq:tcg)
     gene            complement(616..691)
                     /locus_tag="ACHXWD_004309"
     tRNA            complement(616..691)
                     /locus_tag="ACHXWD_004309"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Ile,seq:cat)
     gene            complement(732..1790)
                     /locus_tag="ACHXWD_004310"
     CDS             complement(732..1790)
                     /locus_tag="ACHXWD_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR7622997.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1898..2138)
                     /locus_tag="ACHXWD_004311"
                     /pseudo
     CDS             complement(1898..2138)
                     /locus_tag="ACHXWD_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2363..>2437)
                     /locus_tag="ACHXWD_004312"
     CDS             complement(2363..>2437)
                     /locus_tag="ACHXWD_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7622998.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          585 a          698 c          560 g          594 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      421 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc
      541 atcggggact tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct
      601 aatggcggta tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
      661 tcgctcgctc tcaccactga gctaaagggc cgggagccgc ataataacga cgcgtaatta
      721 attcttcaat atcatccgtt ctggctgact aaatcctgta cttcccgaac cgtctgctca
      781 aaacgttcag tctccagctc aacgccaatt gcacgacgcc cgagcgccag tgccgctttc
      841 actgtcgaac ccaaccccat gaaaaaatct gcaatcaggt cacccggacg actgctagcg
      901 ctgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc gggatagaac
      961 tgcaccggtt tatgcgtcca cacatcggta tacggcacct gcaccgtcac gccaaaatac
     1021 cgccgcagat gtttatattc actcagcagc tccgcatact gccggttcag tgaggtatac
     1081 gtatccacca gctggtggtg gagcttttcc agttcaccac gccgatgctt ctcttctgcc
     1141 acccgggcaa acagcgcctg taatttcaga taatcgcttt cattcggcaa ctgccactga
     1201 ccggcactga accagtgcga caccatgttt ttctttcctg tggcatctgc aatctgtttt
     1261 gccgttatcc ccagggcagc tcgcgcatca cgaaagtaag aaatcagcgg ggccatcaca
     1321 tgctgtttca gtgcactgcc cttcgccgca tacccggcat ctttcggacg atacggcccc
     1381 tgataatgtt ccgcgaacag aatgcgctct gtggcgggga aatacgcccg caggctttcc
     1441 ttgttgcatc cgttccagcg tccggacggc ttcgcccaga taatatggtt cagcacactg
     1501 aagcgttcac gcatcatgat ttcgatatca gatgccaggc gatgaccaca gaacaggtaa
     1561 agacttccgg caggtttcag cacccgccag aactgcgcca gacactggtc cagccacttc
     1621 aggtaatcat cgtcgccctt ccactggtta tcccagccct caggcttcac tttaaagtac
     1681 ggcgggtccg tgactatcag gtcaacagaa ttttcgggta acgaccggat aaattccagg
     1741 cagtcggcgt tgattaactc acaactggat atttttacag tattaagcat ggatcattaa
     1801 gcctgtctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
     1861 tgaacccaac gcatgagcag atggctggtg ggtgccccta acacccacca gccgcccatt
     1921 taccacaaat aaaaaagcct tcactgcgga aggcgtctgt aacaaccgaa ctgatagtct
     1981 gccagacccg ccataaccag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
     2041 gtattctgcg ctatctcccc gactgtcgcc ggttcggtaa cgcttaattc attaaacacc
     2101 actctggcgg tttctgtcat atcctgctgt tttagcatgt cttttccttt tccggttaac
     2161 gtgacacacc aataactctt gtcgaaaaag ccagcaagct gaaagacagg tattcaccgc
     2221 caccagcgcg tttactgtac tgacgcgatt tcagtcataa aaaacccgct cggcggcggg
     2281 ttgtagaaac tcttctaacg tcaggcataa aacgcccatc gttatgacga atttaccaca
     2341 gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg
     2401 cttcttcaat atcaaaccgg gtcaccagcg catcata
//
LOCUS       DAZSBF010000373         2422 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZSBF010000373 DAZSBF010000000
VERSION     DAZSBF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.378"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1365
                     /locus_tag="ACHXWD_004313"
     CDS             <1..1365
                     /locus_tag="ACHXWD_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFR7622999.1"
                     /translation="GSGYHPGNPAGYCPANESGTMRCRGLIALLIWGQSVAAADLGTW
                     GDLWPVKEPDMLTVIMQRLTALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKY
                     SSRLFDPSVRLAADIRDNEGRVAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQ
                     KGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGR
                     KYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSR
                     NGESLLADNMQDGVRIARDNPDSGVVVSIAGEGRPWNPGAITDGRVWGDIPDNSVQPG
                     AGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVREIAGQ
                     ERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKEKT
                     PGGD"
     gene            1385..2132
                     /gene="traX"
                     /locus_tag="ACHXWD_004314"
                     /pseudo
     CDS             1385..2132
                     /gene="traX"
                     /locus_tag="ACHXWD_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            2187..>2422
                     /gene="finO"
                     /locus_tag="ACHXWD_004315"
     CDS             2187..>2422
                     /gene="finO"
                     /locus_tag="ACHXWD_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR7623000.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNT"
BASE COUNT          524 a          618 c          827 g          453 t
ORIGIN      
        1 ggctccggat atcacccggg aaatccagca ggatattgcc cggcgaatga gagcggaacc
       61 atgagatgcc gggggctgat tgccctgctg atatgggggc agagtgtggc cgccgccgat
      121 cttggcacct ggggtgatct gtggccggta aaggagccgg acatgctgac ggtgatcatg
      181 cagcgcctga cggcactgga gcagtccggt gagatgggcc ggaaaatgga tgcctttaag
      241 gagcgggtga tccgcaacag tctgcggccc cctgccgtgc ccggtatcgg acgcacggag
      301 aaatacagca gccggttgtt tgacccgtcc gtcagactgg ctgcggatat ccgggataac
      361 gaagggcggg tggccggctt tgagtcagcc tacgtggccc tgtcgcgtat gaagcagcat
      421 gtgcaggtgt acaccgataa ccgtcagggc tggacggatg ccatcaacaa tgccgtacag
      481 aaaggaactg cccacgatgt gtttgagccg aaaccggacc gggaggtcat gaatgcagag
      541 cggctgttca gtacggcgcg ggagctgcgg gacgtggcgg caggccgtgc cgttctccgt
      601 caggcggggc tggccggggg agacagtcct gcacggttta ttgctccggg acgtaaatat
      661 ccgcaaccgt atgtggcact gccggcgttt gaccgtaacg gcaagtccgc cggtatctgg
      721 ctgaacccgc tgaccacgga tgacggaaac gggctgcggg gattcagcgg tgaaggtcgg
      781 gtaaaaggca gcggggatgc gcagttcgtg gccctgcagg gcagccgtaa cggagaaagc
      841 ctgctggctg ataatatgca ggatggcgtc cggattgccc gtgataatcc tgacagtggt
      901 gtggtggtaa gcattgccgg tgaaggccgt ccgtggaatc ccggtgccat caccgatggc
      961 cgcgtgtggg gggatatccc ggacaacagc gtccagccgg gagccggaaa tggcgaaccg
     1021 gtcacggcag aggtactggc acagcggcag gctgaagagg ccatccgccg tgaaacggaa
     1081 cgtcgcgcag atgaaattgt ccgtaaaatg gcagagaaca aacctgacct gccggacggc
     1141 aaaacagagc tggctgtcag ggaaattgcc gggcaggagc gtgaccgggc tgccataact
     1201 gaacgggaag ccgcgctgcc ggagagtgtg ctgcgtgaat cacaacggga gcgggaagcg
     1261 gtccgggagg ttgcccggga aaatctgctg caggagcgtc tgcagcagat ggagcgggat
     1321 atggttcgtg acctgcagaa agagaaaacc ccgggcggag actgatacag gaagacaaac
     1381 actgatgaca acggataaca cgaacacgac acgtaacgat tcactggctg cacggaccga
     1441 tacctggttg cagtcatttc tggtctggtc acccggacag cgggatatca tcaaaacggt
     1501 ggcactggtg ctgatggtac tggaccacat caatctgata ttccagctga agcaggaatg
     1561 gatgtttctg gccgggcgag gagcctttcc gctttttgcc ctggtgtggg ggctgaatct
     1621 gtcccgtcat gcgcatatcc ggcaaccggc cattaaccgg ctgtggggat ggggaattat
     1681 tgcccagttc gcatattatc tggcaggctt tccctggtat gaggggaata tcctgtttgc
     1741 ctttgcagtg gcggcacagg tgctgacgtg gtgtgaaacg cgcagtgggt ggcgtacagc
     1801 agcggccatt cttctgatgg ccctgttggg ggcctttgtc cggcaccagt tacggcattg
     1861 ccgggctgct gatgctggca gtcagccacc gactgtaccg ggcggaagac agagcggaac
     1921 gtctggcgct ggtcgcctgc ctgctggctg ttattccggc gcttaacctt gccaccagtg
     1981 atgcagcggc ggtggccggt cttgtgatga cggtgctgac cgttggtctg gtgtcgtgtg
     2041 caggaaaatc attaccccgt ttctggcccg gggatttttt cccggtattc tacgcctgtc
     2101 atctggctgt gctgggcgtt ctggcgctgt gacgggtgtg gtatctttga ccgtaacagg
     2161 atgattcgtc agaggcagaa cacagcatga cagagcagaa gcgaccggta ctgacactga
     2221 agcggaaaac ggaaggggag acgcctgtcc gcagccggaa aaccatcatc aatgtcacca
     2281 cgccaccaaa gtggaaggtg aaaaagcaga agctggccga gaaagcagcc cgggaagcag
     2341 agctggcggc aaaaaaagcg caggccagac aggcactgtc catttatctg aacctgccca
     2401 cgctggatga ggccgtgaat ac
//
LOCUS       DAZSBF010000374         2404 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZSBF010000374 DAZSBF010000000
VERSION     DAZSBF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.379"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            77..166
                     /locus_tag="ACHXWD_004316"
                     /pseudo
     CDS             77..166
                     /locus_tag="ACHXWD_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            377..643
                     /gene="hipB"
                     /locus_tag="ACHXWD_004317"
     CDS             377..643
                     /gene="hipB"
                     /locus_tag="ACHXWD_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFR7623001.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            643..1964
                     /gene="hipA"
                     /locus_tag="ACHXWD_004318"
                     /pseudo
     CDS             643..1964
                     /gene="hipA"
                     /locus_tag="ACHXWD_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2148..2249
                     /locus_tag="ACHXWD_004319"
                     /pseudo
     CDS             2148..2249
                     /locus_tag="ACHXWD_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          666 a          505 c          620 g          613 t
ORIGIN      
        1 ggagatttgg cgcagtggag tagcaacgcg caggatcgct ggcatcttgg cgtgatggca
       61 ggctacgcca atcagcatta gcctgtgggg gaatgtcggt gtgcaactag gtgataaagg
      121 ctatagcgat actcagggca tgctgggagt gaaatatagc tggtaaaccg tataagccgc
      181 atgtcgagat ggcatgcggc ttaatattgc caacttcaaa aggcttttaa agcctccctt
      241 ttatccgcga tcgcggatat cgcagcgttt atcccgtaca gcggataaga tgtgtttcca
      301 gattgacttt atcctcacta aaggataaaa ctgataatat ccccttaagc ggataaattt
      361 gctgtggacg tatgacatga tgagctttca gaagatctat agcccaacgc aattggcgaa
      421 tgcaatgaaa ctggttcgcc agcaaaatgg ctggacgcag agcgagctgg cgaaaaaaat
      481 tggcattaag caggcgacga tttccaattt cgaaaacaac cctgacaata ccacgctgac
      541 gacctttttt aagattttac agtcgcttga actctcaatg acgctatgcg acgcgaaaaa
      601 tgcctcgcca gaagcaacag aacagcaaga tctggagtgg taatgcctaa acttgtcact
      661 tggatgaaca accagcgggt aggcgagtta acgaagttag ccaacggcgc gcacaccttt
      721 aagtatgcac cggagtggtt agccagccgt tatgccagac cgttgtcact ttcgctgcca
      781 ttgcagaggg ggaatatcac ctctgatgcc gtatttaact tcttcgataa cctgttaccc
      841 gatagcccga ttgtacgtga ccggatcgtt aaacgttatc atgccaaatc cagacaaccg
      901 tttgatttgt tgtcagaaat agggcgagac agcgttggtg ccgtgacgtt aatacccgaa
      961 gacgaaaccg taacgcgtcc gataatggca tgggaaaagc ttactgaagc cagacttgaa
     1021 gaagtattaa cggcttataa agcagatatc ccgctaggca tgattagaga agaaaatgac
     1081 tttcgcatct cggttgctgg cgcacaggag aagacagcac tgctcagaat aggcaatgac
     1141 tggtgcattc cgaaaggaat aacgccgacg acgcacatca ttaaattacc gattggcgaa
     1201 atcaggcagc ccaatgcgac gctcgatctc agccaaagcg ttgataatga gtattactgt
     1261 ctgctgctgg cgaaagaact tgggttgaat gttccggacg cagaaatcat taaagcggga
     1321 agggtgcgcg cgttagcggt cgaacgtttt gacaggcgtt ggaatactga gcgaacggtt
     1381 ttacttcgct tgccacagga ggatatgtgt cagacattcg gtttaccttc atcggtgaaa
     1441 tatgaatcag atggaggccc aggcatcgcg cggatcatgg cgtttttgat ggggtccagc
     1501 gaggcgctga aagatcgcta tgattttatg aagtttcaag tgttccagtg gttgattggc
     1561 gcaacggatg gccatgcaaa aaacttctcc gtatttattc aggctggcgg cagttatcga
     1621 ctcacgccat tttacgacat catttcagca tttccggtcc ttggcggtac gggaataaca
     1681 tcagcgatct caaactggca atggggctta acgcatccaa aggcaaaaaa acggcaatcg
     1741 ataaaattta tccgcgacat tttttggcga cagcaaaggt gctgagattc ccggaagtgc
     1801 agatgcatga aatcctaagt gactttgcca gaatgattcc ggcagcactg gataacgtga
     1861 agacttcatt accgacagat tttccggaga acgtggtgac ggcagttgaa accaatgtgt
     1921 tgaggttgca tggacggtta agccgagaat acggtagtaa gtgagatatg gggtttggtc
     1981 gttgttaatg agcaatcatg accggccaac gactggattc tcccttggtt tatgtttgtg
     2041 cgcataatgt atattatgtt aaatcatgtc tgagtgatgc gaagtgcttt tctggtagtc
     2101 gttattcgtt cagattaggt gtccttgtga tttaaagatg ttatcgtggc acgaatatac
     2161 tgccattcca ggctcgttat attgctctcg ggcagtccgt agctggtact gcaaacgctg
     2221 atgcgacctt caaagttcaa tatctataat tcaatcttaa ggtggctata cgtcatctga
     2281 aattgaaaca gggtgtgtgt ggcatggcta cgcacacccg agcaattcac caatgtgaat
     2341 ctcattatta atgaggagtg gtttgtattt ggagcgctat gaaaaatcac ataatatcta
     2401 aact
//
LOCUS       DAZSBF010000375         2389 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZSBF010000375 DAZSBF010000000
VERSION     DAZSBF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.380"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..649)
                     /gene="icsB"
                     /locus_tag="ACHXWD_004320"
     CDS             complement(<1..649)
                     /gene="icsB"
                     /locus_tag="ACHXWD_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR7623002.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRN"
     gene            963..>2389
                     /locus_tag="ACHXWD_004321"
     CDS             963..>2389
                     /locus_tag="ACHXWD_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFR7623003.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVNKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSS"
BASE COUNT          809 a          335 c          512 g          733 t
ORIGIN      
        1 agtttctaac ctcttggcct gcatcaagtc tttcggctgt ttggtctgaa atcatattta
       61 gtttgtcttg tttataggta tcgacactgt aacctgagcg tttttcaaaa aggtagttta
      121 tttctgtatc ttttgtaaca ttcccgccgg gataccagct gagataggta tgagttggtt
      181 cctgtagtaa attgtttttt atactgatac tggtatgccc taaatgggtt atgcctctat
      241 ttccccagca acgaaaaact aaatgtcttt caagattttt ggctgtaccg atgccatgaa
      301 aacgctctgc ggtattcaac attaatttga tgtctttacc tgtaaaagaa tgttgaattg
      361 aatttttgac tgcttcatag gcggcaaacg cactttctgc agatttttcg ctagctaatg
      421 tcttgaggaa aagcaataag gatttttgat tcgctagtcg ataatttttt agttgttcat
      481 ttaatatctg tgaatttatc tttgcattaa tagtgcttaa tgttctgaaa aagagatcct
      541 ttaggttgaa tttctgtgct accaatatag gtccatgctc tgtatcttca acgcgtatag
      601 gcccttttgt attgcttgcg tcaatgaaat tgctaatttt gaggatcata ctttattaac
      661 tctccattac gtggtgattg tggggatgta aaatgtattt gatatataat atattcaatt
      721 aataaattta gaaacttgag cctgttaaca taatcaaatt ttcttttgtt gtacataata
      781 tgtacctcgt gagcatatgt agtgctcgtt tcatcatgaa atcccacaag ttaaagtgtc
      841 tgatatatca ggctcggagt gttatagaaa aagagagaac cctgttgaat aagtatcttt
      901 tttgttgtaa aacgaggtac aaaactgcaa tcaatgcccc atttatgact gaggataatt
      961 aaatgcacat aactaatttg ggattgcatc aggtttcatt tcaaagcgga gattcctata
     1021 aaggcgcaga agaaaccgga aagcacaaag gtgtaagcgt gatttcatat caaagagtta
     1081 aaaatggaga aagaaataag gggatcgaag cgcttaatcg attatattta cagaaccaaa
     1141 caagcttaac aggaaaaagc cttttgtttg cgcgtgacag agctgaagtt ttttacgagg
     1201 caataaagtt ggcaggtggt gatacgtcaa aaattaaagc catgatggaa cgattagata
     1261 cctataaact tggtgaagtt aataaaagac atattaatga gcttaataaa gtaataagtg
     1321 aagagataag agcacagcta ggcattaaaa ataaaaagga attgcaaacg aaaataaaac
     1381 aaatatttac tgattattta aataataaga attggggacc ggtcaacaag aatattagtc
     1441 atcatgggaa aaattatggt tttcaattaa cacctgcctc tcatatgaag atagggaata
     1501 aaaatatatt tgtcaaagag tataatggaa aaggaatttg ttgtgcttct acgagagaga
     1561 gtgatcatat cgcgaatatg tggctttcaa aagtggtgga tgacgagggg aaggaaattt
     1621 tttcgggtat cagacatgga gttatatcag cctatggatt gaaaaaaaac tcatctgaaa
     1681 gagctgttgc tgctcgtaat aaagctgagg agttagtaag cgcagcatta tatagcagac
     1741 cagagttatt atcacaggct ttgtctggta aaacagtaga tttaaagatt gtttcgactt
     1801 ctcttctgac gccgaccagt ttaaccgggg gagaggagag tatgttaaaa gatcaggtga
     1861 acgcattaaa agggttaaac tctaaaaggg gggagccaac gaaattatta attcggaata
     1921 gtgatggtct tctgaaagaa gtaagcgtta atctaaaagt ggtgacattt aactttggtg
     1981 taaatgaatt agcgctaaaa atgggcttag gctggaggaa tgttgacaag cttaatgatg
     2041 aatcaatatg ttctttgttg ggggataatt tcctcaaaaa tggtgtgatt ggaggttggg
     2101 ctgcagaagc aatagaaaaa aatccaccat gtaagaatga tgtgatatat ctggctaacc
     2161 agataaaaga gattataaac aagaagttac aaaaaaatga taatggagag ccgtataaat
     2221 tgtcacaaag aatgactcta ttggcttata ctataggtgc tgtaccttgc tggaattgta
     2281 agagtgggaa ggacagaaca ggtatgcagg atgctgaaat taaaagagaa ataataagaa
     2341 aacatgaaac aggtcagttt tctcaattaa atagtaaatt atcctcaga
//
LOCUS       DAZSBF010000376         2339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZSBF010000376 DAZSBF010000000
VERSION     DAZSBF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.381"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            182..808
                     /locus_tag="ACHXWD_004322"
     CDS             182..808
                     /locus_tag="ACHXWD_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR7623004.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..>2339
                     /locus_tag="ACHXWD_004323"
     CDS             829..>2339
                     /locus_tag="ACHXWD_004323"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR7623005.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINF"
BASE COUNT          625 a          500 c          613 g          601 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttggtttatc ggcgttaaat cttttctcat
       61 acactatcgg gttagggaaa atatctggtg ttggggacag agtcagcagc gtattagttg
      121 aatgcttaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaga tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtctaac aattgcccct gcgttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc cagcgagcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg cggcctgtat ggcgatctca actataccgc
      541 ggacacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgctgta ttggctgtag
      661 cgcctgtact acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtctttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacctcact ttctactttt
     1081 accaaaggaa atttagtcgt cgatgcccat atgggtggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaggcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag cctggaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgattgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta ttggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggtggtc cgattgaaac gggggaaatt ccgccaggca atcagaacac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag ccaactttgt ccataccacc
     1861 atcttcccga acggtccgaa agattttgaa gataaagacg atggtcgtgt gattggtaac
     1921 ctggtgggtc tgaatctgtt cgatgactac ggcctatggt gtaactacgg gcagttgcat
     1981 cgcgacttta cctattgtta cagcaaaggt gtgttcaagc gtgttctgcc tgctgaagag
     2041 tatgcggaaa ttcgctggga tcaactggaa gcgggtgacg ttaacttcat taaaattaaa
     2101 gatttttact accgtctggc gcatcgtgtg ggtgagctga gtcacctggc tgatggttca
     2161 tatgccatcg cagaacgctg gaatttgggt gaagagtact ggggctacgc gaaaaataaa
     2221 ctctggtcgc cgtttggcta tccggttcac catgccaatg aagcgtcagc gcaggtcggt
     2281 tccattgtta actgtatgtt caaccgtgac tgcatgacgc atacccatat caactttat
//
LOCUS       DAZSBF010000377         2330 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZSBF010000377 DAZSBF010000000
VERSION     DAZSBF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.382"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            545..1543
                     /locus_tag="ACHXWD_004324"
     CDS             545..1543
                     /locus_tag="ACHXWD_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR7623006.1"
                     /translation="MKNPELKNLTDYSPADKPWDEHRSFSDDVGGIYLRAAGFEHYGE
                     RVGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPK
                     IVTEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFKRLKERKEFRHVLGWVRTTEVT
                     RGKDGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWRDCLRVAYDPNVDIRVVKSRK
                     PKDDESLPCVVAEQLRSAVVETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGAL
                     KNVLQLERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRQLSGED"
     gene            complement(1837..2271)
                     /locus_tag="ACHXWD_004325"
     CDS             complement(1837..2271)
                     /locus_tag="ACHXWD_004325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623007.1"
                     /translation="MAVRARLYDYTSCPDFCGFVSPEHWRNVGSFCAAPPSTEARSSG
                     NRTANHSAVFVPQLFRAIDKLAVGESHEVRTYTISTSFRLCAVCDAVKTADNRQKNPP
                     VSGGRQSQFPDDSCFEVLLYPLNLPQEHSKNNSDDGTGCVIV"
BASE COUNT          531 a          547 c          676 g          576 t
ORIGIN      
        1 aaaaaaaaat tctgcgtccc gctaactgtt cccgaactcg tcaaacagaa aatcgcgggg
       61 gcccctccgg ggttccgatt ttgtgattga cgacggcacg gaaaaaaaca ctggggatgg
      121 tggttggtgt cacgcagtga ggccaaccac ctgcaagacg gtttttgttt ttcacaaaat
      181 cggtggaaaa acctgtgagt gttttgcggg gaaaacgctt ttgtggttgt tgtttttgtt
      241 gtccgaaaat cagctcagat tttgagcaaa tctcgcgcgc gttccttgta tttatactta
      301 agggataaat gcgatttttt gaatcgcctc tatcccttgc catatctggc ctgagcgcgg
      361 atttgcgtgt aaataggttt gagagtattt tgtcgggagt ggttataaat tagcccctgc
      421 gagtcttcca ccactccagg ggcgcgacca cctagaacat gaactgattt gacgacaatt
      481 ggtacaggtg atacaggaat gataacacgc aatttcgtct gtcgctcgca aggggacggc
      541 agacatgaaa aatcctgaac ttaagaatct gacggattac tctccagcag ataaaccgtg
      601 ggatgaacac cgaagctttt ccgacgatgt gggcggtatt tatctgcgtg ctgccggatt
      661 tgagcactac ggcgaacgtg tcggggcttg tagtggtatt ttgcggtttg ggtggtcaac
      721 aaacacagag accggagaaa cgcgcctgcg gttgcgtgat gcacggttct gtcgcgtccg
      781 gcactgtccg atctgtcagt ggaggcgttc catgatgtgg caagcccgtt tctatcagtc
      841 cctgccaaag attgtgacgg aacatcccgg cgcgcgctgg ttgtttctca cactgacagt
      901 gcgcaactgt tccattgacg cgcttggtga gacgctgacc gcgatgaatg cggggtttaa
      961 gcggttgaaa gagcgtaagg aatttcggca tgtgttgggc tgggttcgta ccacggaagt
     1021 aacgcgcggc aaggatggta gcgcacatcc ccattttcac actctgctga tggttccccc
     1081 ttcgatgttg tccggccgtg agtatgtgaa acatgcccgt tgggtggaac tgtggcgtga
     1141 ttgtctgcgg gtggcgtatg acccgaatgt ggatatcaga gtggtgaagt cacggaaacc
     1201 aaaagacgat gaatctttac cttgtgttgt tgcggaacag ctgcgcagcg cggtggttga
     1261 aactctgaaa tattccacaa agcctgctga tatgctggct gatcctgaat ggttcctcga
     1321 attaacacgg caggtacaca agcgtcgttt tgttgctacg gggggagcgc ttaagaacgt
     1381 tctgcaactg gagcgggaaa cgaacgagga tttaatcatg gcggatggca tggcagaggg
     1441 tacagatgac ggttcccgtc tggcgttttc gtgggtgccg gttgtcaaaa aataccagcg
     1501 agatcgggcg aaagataaac gtcagctaag tggcgaggat tgagcgcgtt tcaggcgcga
     1561 actattcatg cactgggaga atattcagaa acgcgctcag aagcgttcta acgtgatttt
     1621 tgcagacagg caaggttttg ttcgtttttt cgcgtttgat gcgttcaggg gtgttttaag
     1681 cgcgtttctg tcgcatcccg gcgctaatac catccccatg gttaacggga gctccagaaa
     1741 cgcgcagatg tctttttaac gtgtcccggg caggttgtcg agagtgtcgg ggcgcagccc
     1801 tgaccaggtg gtaatcggat ggctgtgcgt gcgcggttat acgattacac atcctgtccc
     1861 gtcatcggag ttgtttttgc tgtgttcctg cggcaaattg agcgggtata gcaacacctc
     1921 aaaacaacta tcatcaggaa attgactttg acggccccct gacacagggg gatttttttg
     1981 cctgttatct gcggttttca ccgcgtcgca gaccgcgcac aatcgaaacg aagttgagat
     2041 tgtataagtg cgcacttcgt gagattcccc gacagccagt ttgtcgatcg cgcggaacag
     2101 ctgcggcacg aaaacggcag aatgatttgc tgtccggttg ccagaactgc gcgcttccgt
     2161 ggagggcggc gcggcgcaaa aactgccgac attgcgccag tgttccggtg acacaaaccc
     2221 gcagaaatcg ggacaggatg tgtaatcgta taaccgcgca cgcacagcca tccgattacc
     2281 acctggtcag ggctgcgacc cgacaccccg ccaacgcttc acggcacgcg
//
LOCUS       DAZSBF010000378         2319 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZSBF010000378 DAZSBF010000000
VERSION     DAZSBF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.383"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            10..228
                     /locus_tag="ACHXWD_004326"
     CDS             10..228
                     /locus_tag="ACHXWD_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR7623008.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            230..595
                     /locus_tag="ACHXWD_004327"
     CDS             230..595
                     /locus_tag="ACHXWD_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR7623009.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            592..1257
                     /locus_tag="ACHXWD_004328"
     CDS             592..1257
                     /locus_tag="ACHXWD_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623010.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1257..1622
                     /locus_tag="ACHXWD_004329"
     CDS             1257..1622
                     /locus_tag="ACHXWD_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR7623011.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2077..2232
                     /locus_tag="ACHXWD_004330"
     CDS             2077..2232
                     /locus_tag="ACHXWD_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR7623012.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          624 a          493 c          624 g          578 t
ORIGIN      
        1 cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag aacaacagaa
       61 ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct aatttttgtc
      121 tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt ggtatgggcg
      181 tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata tggcactgac
      241 gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt attgcggctg
      301 cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt ttgatggaaa
      361 tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc acccatgcaa
      421 tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg atggtcgtcg
      481 tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg ttgaaatgaa
      541 atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg catgaccact
      601 attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga tattgacgat
      661 atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact ggaagcagag
      721 cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt cggtgagtat
      781 gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg aatgcgacaa
      841 cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca agttgaccag
      901 gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg ttgggtacga
      961 ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc gccggcagtg
     1021 ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc agattcatcg
     1081 cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt tgccaacgtc
     1141 actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag taatacgttt
     1201 gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga taaataatga
     1261 ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa tgtattgggc
     1321 aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc atgcgtaagg
     1381 ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca gttcctgctg
     1441 aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt tcctgggata
     1501 aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa gacggcgttg
     1561 aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag gtgaatcaat
     1621 gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc tggtggtgta
     1681 ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg gggtttttga
     1741 aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca agtaaacgta
     1801 tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat agatggtgct
     1861 ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg tgcttgaggc
     1921 tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt aacattacgc
     1981 gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa atgtaggtta
     2041 gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa ggcgatgtta
     2101 atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac gaggaaagac
     2161 ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt agactacgaa
     2221 tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg atgtgtcagg
     2281 catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DAZSBF010000379         2186 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZSBF010000379 DAZSBF010000000
VERSION     DAZSBF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.384"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..595)
                     /locus_tag="ACHXWD_004331"
     CDS             complement(<1..595)
                     /locus_tag="ACHXWD_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623013.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
     gene            complement(1025..1348)
                     /locus_tag="ACHXWD_004332"
     CDS             complement(1025..1348)
                     /locus_tag="ACHXWD_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623014.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            complement(1516..1935)
                     /locus_tag="ACHXWD_004333"
     CDS             complement(1516..1935)
                     /locus_tag="ACHXWD_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623015.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            complement(1935..>2186)
                     /locus_tag="ACHXWD_004334"
     CDS             complement(1935..>2186)
                     /locus_tag="ACHXWD_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623016.1"
                     /translation="SRLAAIKDKYPAMSHTALVGEILKEHIALTPFDAIKGVAEGSKA
                     IRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
BASE COUNT          625 a          463 c          403 g          695 t
ORIGIN      
        1 tcagcttagc cagtcttgat ttcagcaaat taatctcatt ttctcttgct ttcatcagtt
       61 tctctttggc aatttcttca agctcagctt gtgatttatc agcaacaatt agagacagtc
      121 gatgtggtgc tctgacagct ctttctgtac gaacaactct ttgaccttct ggtaaatctg
      181 caatcacttt accaatctct acagggtcat acccccaacc gtcacggtct agtcgagaga
      241 aataagcata agaattggtt tgaagctcca tcgaaataac catttcgtca atttcgtctt
      301 caagcttgga ctgaattttc tcgtaaacac ttctcattgc ttcttgagag tctttaatag
      361 cttggaaata tcggcgttgt agcttcaact tcaaatcaag ttcacaccgt tcgttcatag
      421 aaaaatcaat ttcaactctt ggaatctcga gtggttctgg tggaatatat ggacctttat
      481 ttacttcgtg tgattcccgc caagctctat caaaagattc acgcaaagtt aattcgtgaa
      541 tatcaagtgc acttggtctg tgttcgataa gcatttcatg ttcatttcta atcattcgtt
      601 caccccttag tttatttgtt atttcaattt atagaagcac actcagcaat cagaggtgaa
      661 gagtctgact gaacaagaac ctgccaagtg tgcttttaaa aactgaaaaa gccctaaaca
      721 tttctgtgaa gggcaaagga ttcaataatg aatgaacaag agtacttttt gagatagcct
      781 ctcagactaa cctgaaaggc catgacgaaa agcattgcac ctttccaact cagaatatcc
      841 atccatcgga ttattgaaag aaaaataaga gagaattgct ctcccataat taaaattatg
      901 ccatgcttaa ggttatttgt caacagcttt ttaacaggtt gataacatca attcttattt
      961 accgcattgt taataataca accaacaaat aagccaagca acgtccaagc aattattaaa
     1021 cccattagtg aaccctcaca gaatccgtca ccagaatatt atcactgtac aggattgtca
     1081 cagcaccttt caaaaattgc tctggggaaa tactgtatat gttcgtttgc tcgataacca
     1141 atcttttcat gttgctgtct gtaattggta gcatcattcg aggctcgtta aactccaccg
     1201 actgaataaa gtcagagatg ttttctacat tatcagccat cagctttagt aatttatcaa
     1261 aattaattgc gatacctgca tcaatgaagc tttgcaccat catagcagtg tcgttatcaa
     1321 ccataatttg cattgggtaa ttattcattt ttaaatctcc tatgaaacac acctcagacc
     1381 tcttaaaata gccattctga ggccagtaac catgcgggtt acagagggag ttgcactggc
     1441 tggtaaggta atttatcaaa ttaaggtgca actctgcctg taaagtgcat tctaccgttt
     1501 cttagatggt tatttttaat tgtctttatt aatcaatggc ttaatgtaga aatactgtct
     1561 ccgtgaaata ccatattttt ctgtaatgta tcgctgagat tgaccagcat ttatggcctg
     1621 agtgatatca tcacggttat agcggttacc acgggattta tatttgtcac catccacatc
     1681 ttctgaggta ttgaagtctt caatcagctt aattgcatcg tgaaggtcat cttctgaggc
     1741 ataatcgaag gtatggataa tctcaaaatc accagtatct gctggtgcat tcttccatct
     1801 ggagaagcgg gattttaatt gctggtgtgt gaagggttca gcattacgag ttaaatcttt
     1861 ccatgttccg ttattctcac ggacaaggtg acctctatgg cgataaaacg catgataatc
     1921 aaatgtgcat agcattaaac ccccagctta tccgccagtt gatgaacctt ggcactcaac
     1981 acctgaatca ttgagcaacc tgccactgcg gactgtgctt tgagtagttc cagttggccc
     2041 gttgtgattc tgatggcctt agaaccctct gcaacgccct tgatagcgtc aaacggtgta
     2101 agggctatgt gttccttcaa aatctcacca acaagtgctg tgtggctcat ggctgggtat
     2161 ttgtctttga ttgctgccag tctgga
//
LOCUS       DAZSBF010000380         2185 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZSBF010000380 DAZSBF010000000
VERSION     DAZSBF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.385"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            154..663
                     /locus_tag="ACHXWD_004335"
     CDS             154..663
                     /locus_tag="ACHXWD_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR7623017.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            660..2078
                     /gene="phrB"
                     /locus_tag="ACHXWD_004336"
                     /pseudo
     CDS             660..2078
                     /gene="phrB"
                     /locus_tag="ACHXWD_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
BASE COUNT          535 a          537 c          581 g          532 t
ORIGIN      
        1 aatgaatgca gctcgggaaa tagtagaata attctcactc agatgaagtt gataatattt
       61 ttaccaacaa aatacttccc cgcaaaaact ctgccccata aaattcattt accatcgtct
      121 acacttactc ctttataccg ttcaggagca gtaatgaatc tacaacgatt tgatgacagc
      181 accctaatcc gtatctttgc ccttcatgag ttacatcgac tgaaagaaca tggcttaacg
      241 cgcggggcgc ttctcgatta tcacagccgc tataaactcg tctttctggc gcattctcag
      301 ccggagtacc gcaaacttgg cccgttcgtg gctgatattc accagtggca aaatctggat
      361 gactattaca accagtaccg ccaacgcgta gttgttttgc tttctcaccc cgccaacccg
      421 cgcgatcaca ccaatgtttt gatgcacgtt cagggttatt ttcgcccgca tattgattcc
      481 acagaacgcc agcagctggc tgcgcttatc gacagttatc gccgtggcga gcaaccactt
      541 cttgcgccgc tgatgcgtat caaacactat atggcgcttt atcatgacgc ctggctttca
      601 gggcagcgtt atttcgaact ttggccgcgt gtgattaact tgcgccattc aggagtttta
      661 tgactaccca tctggtctgg tttcgccaag atttacgtct gcacgataat ctcgcactgg
      721 ctgccgcctg ccgcaattcg tctgcacgcg tgctggcgtt gtatatcgct acaccacgcc
      781 agtgggcgac gcataacatg tcgccgcgtc aggctgaact tatcaatgct caactgaatg
      841 ggctacaaat agcgcttgcg gaaaaaggta ttcctttatt gttccgtgaa gtggatgact
      901 ttgtcgccag tgtcgaaata gttaaacagg tgtgcgcgga aaacagcgtt acccacctgt
      961 tttataacta tcagtatgaa gtgaatgagc gggcgcggga tgtggaagtt gaaagagcgc
     1021 tgcgtaacgt ggtgtgtgaa ggatttgatg acagcgtgat cctgccgcct ggcgcggtga
     1081 tgaccggtaa tcacgagatg tacaaagtct ttacgccttt taagaatgcc tggctgaaac
     1141 ggctgcggga agggatgccg gagtgcgtcg ctgcgccaaa agttcgtagt agcggatcga
     1201 tagagccctc gccatccatt acgctgaatt atcctcgtca gtctttcgat actgcgcatt
     1261 ttccggtgga agaaaaagcg gcgattgcgc aattacgcca gttttgccag aacggtgccg
     1321 gagaatatga gcaacaacga gattttccgg cagtggaagg caccagccgt ttgtcggcca
     1381 gcctggcaac gggcgggtta tcgcctcgcc agtgcttgca tcgcttgttg gctgaacagc
     1441 cgcaggcgct ggacggtggg gtcggtagtg tctggcttaa tgagctgatc tggcgcgagt
     1501 tttaccgtca cctgataacg tatcacccct cgttgtgtaa acatcgtcca attattgcct
     1561 agacggatcg tgtacagtgg cagagcaatc ccgcacattt acaggcctgg caggaaggca
     1621 aaacgggata cccgattgtt gatgccgcta tgcgtcagct taacagcact ggctggatgc
     1681 ataacaggct acggatgatt acagccagtt ttctggtgaa agatttattg atcgactggc
     1741 gcgaaggcga gcgatatttc atgtcgcagc tgattgatgg tgatttggca gccaataacg
     1801 gtggctggca gtgggccgct tcaaccggaa ccgatgcagc gccgtatttt cgtattttca
     1861 acccgacaac ccagggcgag aaatttgatc tcgaaggtga gtttatccgc cagtggctac
     1921 cggaactacg caatgtaccg gggaaatcgg tgcatgagcc gtggaagtgg gcgcagaaag
     1981 caggtgtgaa gctggattat ccgcaaccga tagtcgagca caaagaggcg agagtacaaa
     2041 cgttggcagc gtatgaggcg gcgcggaagg ggaaataaca gacgcgtcag gcaatcgagc
     2101 ccagatgccg gatgcggcgt gaacgcctta tccagcctac aaatttgtgc aaattcaata
     2161 aattgcagga aatacgtagg cctga
//
LOCUS       DAZSBF010000381         2145 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZSBF010000381 DAZSBF010000000
VERSION     DAZSBF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.386"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..206)
                     /locus_tag="ACHXWD_004337"
     CDS             complement(<1..206)
                     /locus_tag="ACHXWD_004337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623018.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKP"
     gene            143..1642
                     /gene="pitB"
                     /locus_tag="ACHXWD_004338"
     CDS             143..1642
                     /gene="pitB"
                     /locus_tag="ACHXWD_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR7623019.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1691..>2145)
                     /locus_tag="ACHXWD_004339"
     CDS             complement(1691..>2145)
                     /locus_tag="ACHXWD_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HFR7623020.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          501 a          523 c          565 g          556 t
ORIGIN      
        1 ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt tatcgttaaa
       61 aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc gttcgtaaat
      121 tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata tatacacagg
      181 gcttttgtta ttgcttgctc tggcatttgt gttgttctac gaagcaatca atggttttca
      241 tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcaac cacaacttgc
      301 tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac ttagcgttgc
      361 ctatgccatt gtccatatgt tgccaaccga tttgttgctg aatatggggt caacccacgg
      421 cctggcgatg gtcttttcca tgctgctggc ggcgattatc tggaacctgg gaacgtggtt
      481 cttcggttta ccggcctcca gttcgcacac cttgattggt gcgattatcg gcatcggttt
      541 aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc gtgaagtgac
      601 caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg cgggaggcct
      661 gatattcctg ctgcgacgct actggagcgg gacgaaaaag cgtgaccgta ttcaccgcat
      721 tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg ccattctgga cgcgtattgc
      781 gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg gacaaaaagg
      841 gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg tcgtcaatat
      901 gaacgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gtcaccaact tcgaacacta
      961 cctgcaacag catcctgaac tgccgcagaa gttgattgcg atggaacctc cattgcctgc
     1021 agcatcgact gatggcacgc aagtaacaga gtttcactgt catccggcaa atacctttga
     1081 tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg agccgttaag
     1141 cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctctgata cctccgcgaa
     1201 gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa aactgcgcag
     1261 cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg tagcactggc
     1321 gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg gtgagaagat
     1381 tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg cggcagtgtc
     1441 tatcggcctt gccagttata ttgggatgcc tgtctccaca acacacgtcc tctcgtctgc
     1501 ggtagcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg taaccagcat
     1561 cctgatggcg tgggtgttta ctttaccggc ggcaattttt ctttctggtg ggctgtactg
     1621 gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcctggcga tcgggcattt
     1681 ccatttttga ctagtgataa ccacgcgcgg tcataaaatc cgtaatcgct ttttctgcat
     1741 caaccaacac ctgttccagt ggctgattgg catcgatatc aaccagttgt gcaccaccaa
     1801 aggttaactg tggcgttatg gcaatcttcc tcgccagcga ttcccgttta tggtcgggtt
     1861 tacgtgcaca ggcaacttca aggtcaacat tgagtttgat gaccagatca ggcttatggc
     1921 tcgccatcca gtgaaacgct ttacgttcct ggcttgccag ccatgagaca aaacgaccac
     1981 cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc aggaatttgg tcctgaggat
     2041 aacggtcggt tagaacaatt aacccgcgac gacgacaggc aagcatatga cgaaagcgca
     2101 gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactg
//
LOCUS       DAZSBF010000382         2134 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZSBF010000382 DAZSBF010000000
VERSION     DAZSBF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.388"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            9..1202
                     /locus_tag="ACHXWD_004340"
     CDS             9..1202
                     /locus_tag="ACHXWD_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7623021.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            complement(1152..2078)
                     /locus_tag="ACHXWD_004341"
     CDS             complement(1152..2078)
                     /locus_tag="ACHXWD_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR7623022.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
BASE COUNT          469 a          519 c          523 g          623 t
ORIGIN      
        1 acattataat gtcaccaggg atcgaagata cgccccaaaa aacactgtcc tgctggccac
       61 ttgcgttcag tgccgggctt ctcggtatcg gacagaacgg tctgctggtt gtgctccctg
      121 ttctggtcat acagacaaat ctgagtctgt ctgtatgggc tgccctgctg atgcttggct
      181 caatgctgtt tctgccatcg tccccgtggt ggggaaagca aatttcccgt actggcagta
      241 aacctgtggt gttgtgggct ctgggaggat atggcataag ctttaccctg ctagggctgg
      301 gaagcgtgct gatggctacc agcgccataa caacagcggt gggactggga atattaatca
      361 tcgcccggat cgcctacggg ctgaccgtgt cggcaatggt gccagcctgt caggtctggg
      421 cattgcagag agctggagaa gggaatcgca tggccgctct ggcaaccatc agctccggcc
      481 tgagttgcgg caggctattc gggccgctgt gcgcggcagc aatgctggcc attcatcctc
      541 tggcgccact ggggctactg atggcagcac cagtgctggc gctgctgatg ctgctgcggt
      601 tgcccggcac accaccacag cccacaccgg agtgcaagag cgtcagtctg aagcgggatt
      661 gtctgcctta tctgctttgc gcaatattac tggctgcggc ggtgagcatg atgcagcttg
      721 gactttcgcc tgcccttact cgccagttcg tcacggatac caccgccatt agccaacagg
      781 tggcctggct gttgggtctg tccgcagtag ctgcgcttat cgcgcagttc ggggtactcc
      841 gtccgcagcg cctgactccg gtggccctgc tcctgagtgc cggagtgttg atgagtggtg
      901 gtctggctat catgctctcc gaacagctat ggttgtttta cccgggctgt gcagtgctgt
      961 catttggagc agctctggca acacccgctt atcaacttct actgaatgat aagctggctg
     1021 atggcgcagg cgcgggctgg ctcgctacca gtcacacact tggctatgga ctgtgcgctt
     1081 tgctggtgcc gctggtatcg aaaacaggtg tcgcaatcgc gctgattatg gcagcattat
     1141 ttgcaactat attatttacc attgtgtctg tatttatctg gcattaccgt actatcaaat
     1201 aaagatatga tcatcacctt actggagtaa atcagaggat atgagagagg acctgcgtct
     1261 gatgaaagca tgtcaggcag tgttctggct tgctgccccc gcccatcagg tatagtgaat
     1321 tcaaccagtt gctgcggtgt aatctccatg attctttcgt gcaacaacct atgatcccgt
     1381 ttaccattaa tattcagcgt tgctctgtta ttaaatttca ttctgacaag ccagtcagct
     1441 tttattccgg gagtctgctc tgcgcgttca aacaggtcat aaaaatcgcc ttcacgatca
     1501 gaaattgaca atacatgggt atcagggcat tggcgagcca gttctgttgc tttattatat
     1561 ccttcaaccc aacgccaggt ttctttttca ctgacatgtt ttttatgaag taaatcattg
     1621 cagatttctt gtcgggtatt tttactggtt tttaattcat ccctgaacca ctgatagtca
     1681 tcataaacac cgaggcaaac acctgactca ctgatgacga gttgaggatg aagaaataag
     1741 tttctttcat ctttatgttt tttgtgacca actccttgtt tttctttctg accagaataa
     1801 ttaagttctg ttgtatcctg taaaactaaa actcttttaa actgctgagc tctttcagtg
     1861 atattttctt tgtgaggagc cataatttta ttagcagaga ctttatcgtt tgaaaaacaa
     1921 cgataagctg cctttgtttc actccaggta cggcaggaaa caggaatact ttcttctggt
     1981 gaacgactga ggctattgag gatatgtttt actcttttat caagtcttga gtcccctata
     2041 tccagtgatt ttagctcctg aataacccag ttttccataa cacctctgaa ataatctctg
     2101 tcttttcaat ataatactat tatttcaact tgtg
//
LOCUS       DAZSBF010000383         2093 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZSBF010000383 DAZSBF010000000
VERSION     DAZSBF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.389"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            82..462
                     /locus_tag="ACHXWD_004342"
     CDS             82..462
                     /locus_tag="ACHXWD_004342"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7623023.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            459..806
                     /gene="tnpB"
                     /locus_tag="ACHXWD_004343"
     CDS             459..806
                     /gene="tnpB"
                     /locus_tag="ACHXWD_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR7623024.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            856..>2093
                     /locus_tag="ACHXWD_004344"
     CDS             856..>2093
                     /locus_tag="ACHXWD_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR7623025.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPMEEALHGYVLTDGKLHVDDTPVPVLLPGNKKTKTGRLWAYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFA"
BASE COUNT          488 a          586 c          608 g          411 t
ORIGIN      
        1 gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta
       61 gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc
      121 cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata
      181 tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga
      241 caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca gctacttcct
      301 gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc gaaaaccctc
      361 agtatcagct gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc
      421 gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga
      481 tcaaaatttg gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg
      541 cgaaagtaca gacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg
      601 gccgcagcgg cagtcaggtt aaactgctgt ggtccaccag tgacggactg tgcctcctga
      661 ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc
      721 ttacacaggc gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc
      781 tgctgacctc cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg
      841 gtaaaatacg ggcttatgaa cgacatctct tctgacgaca tcttcctgct gaaacagcgc
      901 ctggccgaac aggaagcgct gatccacgcc ctgcaggaaa agcttagcaa ccgggagcgc
      961 gaaatagacc atctgcaggc gcagctggat aaactccgcc ggatgaactt cggcagtcgt
     1021 tccgaaaaag tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa
     1081 gagagcgata cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag
     1141 acccgcaccc gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct
     1201 gcggcgccgt gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccgcc
     1261 gaacagctgg agttgatgcg tagcgccttc cgggttatcc ggacggtacg ggaaaaacat
     1321 gcctgtactc agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg
     1381 ggtatcgccg gaccggggct gctggcccgc gtgctgacct cgaagtatgc agagcacacc
     1441 ccgctgtatc gccagtcaga aatatacggc cggcaaggtg tggagctgag ccgttcactg
     1501 ctgtcgggct gggtggatgc atgctgccgg ctgctgtctc cgatggaaga ggcgcttcat
     1561 ggctatgtgc tgactgacgg taagctccat gttgatgaca cgcctgtccc ggtgctgttg
     1621 ccaggcaata agaaaacgaa gaccgggcgg ttgtgggcgt atgttcgtga cgaccgtaac
     1681 gccgggtcaa cgctggcgcc ggcggtgtgg ttcgcttaca gcccggacag aaaaggcatc
     1741 catccgcaga cccatcttgc ggggttcagt ggtgtactgc aggcggatgc atacgccggg
     1801 ttcaacgagc tgtaccggga tggccggata acggaagccg cctgttgggc tcacgcccgc
     1861 cgtaaaatcc acgatgtgca cgttcgcacc ccgtcagccc tgacggagga agcgctgaaa
     1921 cggatcggcg aactgtacgc catcgaggca gagataaggg gaatgacggc ggaacagcgc
     1981 cttgccgaac gtcagttgaa aacgaaaccg ctgctgaaat ccctggaaag ctggctgcgt
     2041 gaaaagatga aaaccctgtc gcgacactca gaactggcga aagcgttcgc ata
//
LOCUS       DAZSBF010000384         2089 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZSBF010000384 DAZSBF010000000
VERSION     DAZSBF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.390"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHXWD_004345"
     CDS             complement(60..1025)
                     /locus_tag="ACHXWD_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR7623026.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQIAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHXWD_004346"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHXWD_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623027.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          549 c          437 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcaatc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag acttgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcactccc gaacggctga gcccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZSBF010000385         2064 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZSBF010000385 DAZSBF010000000
VERSION     DAZSBF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.391"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            111..1244
                     /gene="ompN"
                     /locus_tag="ACHXWD_004347"
     CDS             111..1244
                     /gene="ompN"
                     /locus_tag="ACHXWD_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR7623028.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1385..1819
                     /gene="uspF"
                     /locus_tag="ACHXWD_004348"
     CDS             1385..1819
                     /gene="uspF"
                     /locus_tag="ACHXWD_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR7623029.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          576 a          471 c          462 g          555 t
ORIGIN      
        1 ttaaggcgta acaaatgata tttatcggct aactgaactt ctcctttagg atgttttcac
       61 tccccggaat ggggataaaa ggcaaataaa ataactaaag gatttattca atgaaaagca
      121 aagtactggc acttttaatt cctgccctgc tcgccgcagg tgctgcacat gcagccgaag
      181 tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc ctgcattatt
      241 tttctgataa ttcagcgaaa gatggcgatc agagctatgc gcgtctgggt tttaaaggcg
      301 aaacccaaat taacgatcaa ctcactggct acggtcaatg ggaatacaat attcaggcaa
      361 acaacactga atcttcaaaa aaccagtcat ggacccgtct ggcatttgcc gggctgaaat
      421 ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac gacatcgaag
      481 gctggaccga tatgctgcct gaatttggcg gtgactctta taccaatgca gacaacttta
      541 tgactggtcg tgccaacggc gtcgcgactt atcgtaatac tgatttcttc ggtctggtaa
      601 atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggtgccagt aatggtcagg
      661 aaggcaccaa caacggacgt gatgttcgcc atgaaaatgg tgacggctgg ggtctttcca
      721 caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctca tctgaccgca
      781 ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg tggactgctg
      841 ggctaaaata cgatgctaac aatatttacc tggcaaccat gtattcagaa acgcgtaata
      901 tgaccccgtt tggcgacagc gattatgctg ttgcaaacaa aacccagaac tttgaagtca
      961 ctgcacagta ccagtttgat tttggtctgc gtccggcagt ctctttcctg atgtctaaag
     1021 gccgtgacct gcacgctgcc gatggtgcag acaacccggc aggtgttgat gataaagatc
     1081 ttgttaaata cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacttatg
     1141 ttgactataa aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct
     1201 ctaccgatga tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg
     1261 ttatggcggg ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc
     1321 cgtcattaca caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg
     1381 ttttatgaac agaacgattc ttgtccctat cgatatttcc gattcagaat taactcaacg
     1441 cgtgattagc cacgttgagg cagaggcaaa gattgacgat gcagaggttc atttcctgac
     1501 ggtaatacct tcactgcctt actatgcctc tctgggttta gcgtattccg cagaattacc
     1561 ggcaatggat gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt
     1621 taaactgcca accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat
     1681 tctggaattg gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga
     1741 tatcaccact tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc
     1801 cgtgctggtt gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt
     1861 cgcaaatgtg ctgacatttt cccctctaat ccgtaccata cacgccacag ttttatatca
     1921 gatttcttat gctgggcgtt ccggcatgac gcatactctt ctgatgccat ataacgaatt
     1981 gagtcgcttt taaatgtcgc aaaatcaaga aattagtaag aaagaacaat acaacctgaa
     2041 caagtaaggg caaaaatcac aact
//
LOCUS       DAZSBF010000386         2025 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZSBF010000386 DAZSBF010000000
VERSION     DAZSBF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.392"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(147..617)
                     /gene="argR"
                     /locus_tag="ACHXWD_004349"
     CDS             complement(147..617)
                     /gene="argR"
                     /locus_tag="ACHXWD_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR7623030.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            1052..1990
                     /gene="mdh"
                     /locus_tag="ACHXWD_004350"
     CDS             1052..1990
                     /gene="mdh"
                     /locus_tag="ACHXWD_004350"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR7623031.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT          523 a          439 c          521 g          542 t
ORIGIN      
        1 atgtgctttt tttaccgtat gtattcattg tgtgaatgat atgtcgcggt aaaacgcact
       61 attcgttaaa ttaatcgcag gggaaagcag ggggttgaga ggaataagca acattttccc
      121 cgccgccaga agcgacgggg cagagattaa agctcctggt cgaacagctc taaaatcgct
      181 tcgtacaggt ctttgacggt gaaaccgtta gcaggggtgg taaagatggt gtcatcgcca
      241 gcgatggtgc ccagaatacc ttctgctttg cccagtgagt ccagcaggcg agcaattaac
      301 tgcgccgcgc cagggctggt atgaatcacg acaactgcat cgttgtagtc gatatccagc
      361 accagattct tcaatggact ggaggtggtt ggtacaccca gttcagctgg caggcagtaa
      421 accatttcca ttttggcatt gcgtgtacgt acagcaccaa acttggtcaa catccgcgag
      481 actttagact gattaatatt gtcaaagcct tgctcctgca acgcggcgac gatttcgccc
      541 tgggagctaa atttctcttc tttaagtaat gctttaaatg ctttaactag ttcttcttgc
      601 ttagccgagc ttcgcataag tcacccgata tggtggttga tacaacatta ttgtgcatac
      661 agatgaattt ttatgcaaac agtcagccct gaagaaggct gaaataatgt tatgaaagag
      721 cgggatttta tcaaatttcg ttattgagaa acatgcctgc gtcacggcat gcaaattctg
      781 tttaaaagta aattaattgt tatcaaattg atgttgtttt ggctgaacgg tagggtatat
      841 tgtcaccacc tgttggaatg ttgcgctaat gcataagcga ctgttaatta cgtaagttag
      901 gttcctgatt acggcaatta aatgcataaa cgctaaactt gcgtgactac acattcttga
      961 gatgtggtca ttgtaaacgg caattttgtg gattaaggtc gcggcagcgg agcaacatat
     1021 cttagtttat caatataata aggagtttag gatgaaagtc gcagtcctcg gcgctgctgg
     1081 cggtattggc caggcgcttg cactactgtt aaaaacccaa ctgccttcag gttcagaact
     1141 ctctctgtat gatatcgctc cagtgactcc cggtgtggct gtcgatctga gccatatccc
     1201 tactgctgtg aaaatcaaag gtttttctgg tgaagatgcg actccggcgc tggaaggcgc
     1261 agatgtcgtt cttatctctg caggtgtagc gcgtaaaccg ggtatggatc gttccgacct
     1321 gtttaacgtt aacgccggca tcgtgaaaaa cctggtacag caagttgcga aaacctgccc
     1381 gaaagcgtgc attggtatta tcactaaccc ggttaacacc acagttgcga ttgctgctga
     1441 agtgctgaaa aaagccggtg tttatgacaa aaacaaactg ttcggcgtta ccacgctgga
     1501 catcattcgt tccaacactt ttgttgcgga actgaaaggc aaacagccag gcgaagttga
     1561 agtgccggtt attggcggtc actctggtgt taccattctg ccgctgctgt cacaggttcc
     1621 tggcgttagt tttaccgagc aggaagtggc tgatctgacc aaacgcatcc agaacgcggg
     1681 tactgaagtg gttgaagcga aagccggtgg cgggtctgca accctgtcta tgggccaggc
     1741 agctgcacgt tttggtctgt ctctggttcg tgcactgcag ggcgaacaag gcgttgtcga
     1801 atgtgcctac gttgaaggcg atggtcagta cgcccgtttc ttctctcaac cgctgctgct
     1861 gggtaaaaac ggcgtggaag agcgtaaatc tatcggtacc ctgagcgcat ttgaacagaa
     1921 cgcgctggaa ggtatgctgg atacactcaa gaaagatatc gccttgggcg aagagttcgt
     1981 taataagtaa ttgattagcg aataataaaa aaccggagca cagac
//
LOCUS       DAZSBF010000387         2013 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZSBF010000387 DAZSBF010000000
VERSION     DAZSBF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.393"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(37..273)
                     /locus_tag="ACHXWD_004351"
     CDS             complement(37..273)
                     /locus_tag="ACHXWD_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR7623032.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="ACHXWD_004352"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="ACHXWD_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR7623033.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHXWD_004353"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHXWD_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR7623034.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..>2013)
                     /locus_tag="ACHXWD_004354"
     CDS             complement(1875..>2013)
                     /locus_tag="ACHXWD_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFR7623035.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          527 a          530 c          439 g          517 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAZSBF010000388         2010 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZSBF010000388 DAZSBF010000000
VERSION     DAZSBF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.394"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(177..1037)
                     /locus_tag="ACHXWD_004355"
     CDS             complement(177..1037)
                     /locus_tag="ACHXWD_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR7623036.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1220..1651)
                     /locus_tag="ACHXWD_004356"
                     /pseudo
     CDS             complement(1220..1651)
                     /locus_tag="ACHXWD_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1717..>2010
                     /locus_tag="ACHXWD_004357"
     CDS             1717..>2010
                     /locus_tag="ACHXWD_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFR7623037.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR"
BASE COUNT          500 a          517 c          465 g          528 t
ORIGIN      
        1 gccggacatc agcgctatct ctgctctcag gggtctgacg ctcagtggaa cgaaaactca
       61 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      121 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      181 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      241 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      301 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
      361 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      421 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      481 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      541 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      601 agctccttcg gtcctccgat cgttgtcaga agtaagttgg cagcagtgtt atcactcatg
      661 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      721 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      781 tgcccggcgt caacacggga taataccgca ccacatagca gaactttaaa agtgctcatc
      841 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      901 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      961 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacga
     1021 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta ccagggttat
     1081 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1141 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1201 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaatttta taaaccgtgg
     1261 agcgggcaat actgagctga tgagcaattt ccgttgcacc agtgcccttc tgatgaagcg
     1321 tcagcacgac gttcctgtcc acggtacgcc tgcggccaaa tttgattcct ttcagctttg
     1381 cttcctgtcg gccctcattc gtgcgttcta ggatcctccg gcgttcagcc tgtgccacag
     1441 ccgacaggat ggtgaccacc atttgcccca tatcaccgtc ggtactgatc ccgtcatcaa
     1501 tgaaccggac tgccacgccc tgagcgtcaa attcctttat cagttggatc atatcggcag
     1561 tgtcgcggcc aagacggtcg agcttcttaa ccagaatgac atcaccttcc tccaccttca
     1621 tcctcagcaa atccagccct tcccggtctg ttgggcactg ttgcaaatag tcggtggtga
     1681 taaacttatc atcccctttt gctgatggag ctgcacatga acccattcaa aggccggcat
     1741 tttcagcgtg acatcattct gtgggccgta cgctggtact gcaaatacgg catcagttac
     1801 cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg tcgatcactc cacgatttac
     1861 cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc tgcgctggta ctggcgtaac
     1921 ccttccgatc tttgcccgtg gcacatggat gaaacctacg tgaaggtcaa tggccgctgg
     1981 gcgtatctgt accgggccgt cgacagccgg
//
LOCUS       DAZSBF010000389         2005 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZSBF010000389 DAZSBF010000000
VERSION     DAZSBF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.395"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHXWD_004358"
     CDS             <1..389
                     /locus_tag="ACHXWD_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623038.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHXWD_004359"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHXWD_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR7623039.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHXWD_004360"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHXWD_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR7623040.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHXWD_004361"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHXWD_004361"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHXWD_004362"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHXWD_004362"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZSBF010000390         1995 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZSBF010000390 DAZSBF010000000
VERSION     DAZSBF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.396"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..326
                     /locus_tag="ACHXWD_004363"
     CDS             <1..326
                     /locus_tag="ACHXWD_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7623041.1"
                     /translation="VITMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGAS
                     YRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMK
                     DSQRA"
     gene            428..1882
                     /gene="amn"
                     /locus_tag="ACHXWD_004364"
     CDS             428..1882
                     /gene="amn"
                     /locus_tag="ACHXWD_004364"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR7623042.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          478 a          495 c          477 g          545 t
ORIGIN      
        1 gagtcattac catggcgctt gaagcacaat ctattttctg gatagttttc ttctccataa
       61 tgctggcaaa cattgcgcat gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa
      121 tgtttggtgc cagttatcgc tatagtggcg ctggagtcgg ttatcaggtt gccagtgtgg
      181 ttggcggtgg atttacacct tttattgccg ctgcactcat cacttacttt gccgggaact
      241 ggcatagcgt cgccatttat ttgctggctg gatgcctgat ttccgcaatg accgctttgt
      301 tgatgaaaga cagtcaacgc gcttgatagc ctggcgaaga tcatccgatc ttcgccttac
      361 acttttgttt cacatttctg tgacatacta tcggatgtgc ggtaattgta tggaacagga
      421 gacacacatg aataataagg gctccggtct gaccccagct caggcactgg ataaactcga
      481 cgcgctgtat gagcaatctg tagtcgcatt acgcaacgcc attggcaact atattacaag
      541 tggcgaatta cctgatgaaa acgcccgcaa acaaggtctt tttgtctatc catcactgac
      601 cgtaacctgg gacggtagca caaccaatcc ccccaaaacg cgcgcatttg gtcgttttac
      661 tcacgcaggc agctacacca ccacgattac tcgccctact ctctttcgtt cgtatcttaa
      721 tgaacaactt acgttgctgt atcaggatta tggtgcgcat atctctgtgc aaccctcgca
      781 gcatgaaatc ccttatcctt atgtcatcga tggctctgaa ttgacacttg atcgctcaat
      841 gagcgctggg ttaactcgct acttcccgac aacagaactg gcgcaaattg gcgatgaaac
      901 tgcagacggc atttatcatc caacggaatt ctccccgcta tcgcattttg atgcgcgccg
      961 cgtcgatttt tccctcgcac ggttgcgcca ttataccggt acgccagttg aacattttca
     1021 gccgttcgtc ttgtttacca actacacacg ttatgtggat gaattcgttc gttggggatg
     1081 cagccagatc ctcgatcctg atagtcctta cattgccctt tcttgtgctg gcgggaactg
     1141 gatcaccgcc gaaaccgaag cgccagaaga agccatttcc gaccttgcat ggaaaaaaca
     1201 ccagatgcca gcatggaatt taattaccgc cgatgctcag ggtattactc tggtgaatat
     1261 tggcgtggga ccatcaaatg ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga
     1321 tgtctggttg atgattggtc actgtggcgg attacgtgaa agtcaggcca ttggcgatta
     1381 tgtacttgca cacgcttatt tacgcgatga ccacgttctt gatgcggttc tgccgcccga
     1441 tattcctatt ccgagcattg ctgaagtgca acgtgcgctt tatgacgcca ccaagctggt
     1501 gagcggcagg cccggtgagg aagtcaaaca gcggctacgt actggtactg tggtaaccac
     1561 agatgacagg aactgggaat tacgttactc agcttctgca cttcgtttta acttaagccg
     1621 ggccgtagca attgatatgg aaagtgcaac cattgccgcg caaggatatc gtttccgcgt
     1681 gccatacggg acactactgt gtgtttcaga taaaccatta catggtgaga ttaaacttcc
     1741 cggtcaggct aaccgttttt atgaaggcgc tatttccgaa catctgcaaa ttggcattcg
     1801 ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat tcgcgtaaat tacgaacctt
     1861 taatgagccg ccgttccgat aataagttct gaaccttctt atcagaaata gatgtgaagg
     1921 agtaagtaag acacctggca aatagcctgc aataatcgtg ggctgtttgc ttccttgggc
     1981 ggatacgagt tttat
//
LOCUS       DAZSBF010000391         1995 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZSBF010000391 DAZSBF010000000
VERSION     DAZSBF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.397"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            270..539
                     /gene="yacA"
                     /locus_tag="ACHXWD_004365"
     CDS             270..539
                     /gene="yacA"
                     /locus_tag="ACHXWD_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFR7623043.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            539..708
                     /gene="yacB"
                     /locus_tag="ACHXWD_004366"
                     /pseudo
     CDS             539..708
                     /gene="yacB"
                     /locus_tag="ACHXWD_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            791..1381
                     /gene="ospG"
                     /locus_tag="ACHXWD_004367"
     CDS             791..1381
                     /gene="ospG"
                     /locus_tag="ACHXWD_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFR7623044.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1738..>1995
                     /locus_tag="ACHXWD_004368"
     CDS             1738..>1995
                     /locus_tag="ACHXWD_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR7623045.1"
                     /translation="MTEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPR"
BASE COUNT          563 a          439 c          471 g          522 t
ORIGIN      
        1 tgactgacga ggtactggcc ctgcgattgt ctgaaaacgg ctcacaactg caccattcat
       61 aatcacatcg cataaaactg tcagcgccag tgataggaca ggtgaaacct gtcaatacgt
      121 accggcttac gccgggcttc ggcggtttta ctcctctgcc atatgcaaca acggagtgcc
      181 gccttacatg ccgctggcgc ggcatatcct ggtgacgata tctgaacagt tatatactgt
      241 gtatatacgt ggtaatgacg aggtggcaaa tggcacaggt taatatgagt gtaagaatcg
      301 acgcagaact gaaggatgct tttatggccg ctgcaaaaag catggaccgt aatggctctc
      361 agttaatccg ggattttatg cgccagaccg ttgaacggca gcacaattct tggttccgtg
      421 accaggttgc ggcaggccgt cagcaactcg aatgcggtga tgtgcttccc catgacatgg
      481 tcgaatcctc tgctgctgca tggcgggatg aaatgagcag gaagattgct gataaatgat
      541 ggagatgttc tggaccatgc tggccatcca ggacaggaag cgcattcgta gtacattgcg
      601 gagcagaacc tgatggccgc cattgaaccg gacgaacgga ttggttattc agccagcagc
      661 ctggcagggc agccgtataa aggccgcaac ggtcgtgtgg aaggaacatc tggaccacac
      721 aaggtagcgt gtaacgttat tttgtgtgaa aatctcttgt gatttttaaa tatcgtaaca
      781 aaggtatgac atgaaaataa catctaccat tattcaaaca ccttttccat ttgagaataa
      841 taattctcat gctggcatag taacggagcc cattctcggt aagttaatag gtcaggggtc
      901 gacagcagaa atctttgaag atgtgaatga ttcatctgct ttgtataaaa agtatgatct
      961 tattggcaac cagtacaatg agattctgga aatggcttgg caagaatctg agctttttaa
     1021 tgctttttat ggcgatgaag catccgttgt tatacagtat ggcggagatg tgtacctccg
     1081 aatgctgcgc gtgcctggga ctccccttag tgacattgat acagctgata tccctgataa
     1141 tatagagagc ctttatctac agttgatatg taaattgaat gagttgagta taatccatta
     1201 cgatcttaat acaggtaata tgctgtatga taaagaaagt gaaagtttat tcccaataga
     1261 ttttcgcaat atttatgctg aatattacgc tgcaaccaaa aaagataaag agattatcga
     1321 ccgacgatta caaatgcgta caaatgattt ttattcgtta ttaaacagga aatatttata
     1381 gccgtatttg ttgatgctat aagccagagg ctcttctatg agaatatggg gaccattccc
     1441 tcgcgatatt aacgtaatat cttaattgta tgcaccgcca gaatgcgttc agtgcgatca
     1501 cggcagcaga caggtaaaaa tagcaacaaa ccacccgaaa aaccgccgcg atcgcgtatg
     1561 ataaatttta accacatgca tacctgtgca gcaaggcgaa tcacgcacgg atttccagcc
     1621 agagacacct gaaacgtaac cggctcattt aaaccgtctg gtttgttacc tccggttcca
     1681 caaaaataat gtccatcatt ttgaagtgga cactatcgta tggaataccg gacctgaatc
     1741 actgaagctt tacgccttca tttcgaagaa catttaccac gggttgtggc cggacgtcgc
     1801 ctgggcgtac caaaatcaac tgtctgtggt atgttcgtgc gcttccgcaa cgctggcctt
     1861 tcatggcctc tgcccgcagg tatgtcggag caggaacttg atgcccttct ttacggaagt
     1921 gcctccacag tacctgtcgt acttactgaa agcaccgtta tgcccaaact cccggtggta
     1981 aaaaaacgtc cccgg
//
LOCUS       DAZSBF010000392         1988 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZSBF010000392 DAZSBF010000000
VERSION     DAZSBF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.398"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(13..783)
                     /gene="spa29"
                     /locus_tag="ACHXWD_004369"
                     /gene_synonym="spaR"
     CDS             complement(13..783)
                     /gene="spa29"
                     /locus_tag="ACHXWD_004369"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFR7623046.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            complement(783..1043)
                     /gene="spaQ"
                     /locus_tag="ACHXWD_004370"
     CDS             complement(783..1043)
                     /gene="spaQ"
                     /locus_tag="ACHXWD_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFR7623047.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            complement(1058..1708)
                     /gene="spa24"
                     /locus_tag="ACHXWD_004371"
                     /gene_synonym="spaP"
     CDS             complement(1058..1708)
                     /gene="spa24"
                     /locus_tag="ACHXWD_004371"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFR7623048.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            complement(1713..>1988)
                     /gene="spa33"
                     /locus_tag="ACHXWD_004372"
                     /gene_synonym="spaO"
     CDS             complement(1713..>1988)
                     /gene="spa33"
                     /locus_tag="ACHXWD_004372"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFR7623049.1"
                     /translation="SESNGESEHTEVSLALFNYDDINVKVDFILLEKNMTINELKMYV
                     ENELFKFPDDIVKHVNIKVNGSLVGHGELVSIEDGYGIEISSWMVKE"
BASE COUNT          794 a          367 c          297 g          530 t
ORIGIN      
        1 tgccataata ttttatctaa caaatagatt tgtgaaaaat ttatgttcac cgaggaaaaa
       61 ttgaacttta gaaaagtaaa tagtagaaga acagatgaaa ataataaata ttgcaagtaa
      121 acttttaata gttaatgata cggaaaaagc attcatctgc ggagcaaatc tcgataatac
      181 accaagtaat acttctgata gtaacaatac tatcataaca ggactggcta aaataacagc
      241 ctgacttgcc aataaagtca gaaatgttaa gatatttgag acacggaaag aacattgaga
      301 aaataacggg catatattat aagacaattg tatggattct aaaataaaga ccataccacc
      361 actgtataga aatacaactg cagaaaaaag attgaaaaat tttgccaact cagacgtatc
      421 aacaccattg gcaggatcaa ttgaactact aagcgttgcc ccacgctggt tgtcaataat
      481 gctaccaaca gcatgaaata tccaaaaggg aagagacaag caaaaagaga gaaggaggcc
      541 aacaattatt tccttgaaca taaggaaata aacatgttca aaaacagaag aacctatgtc
      601 taccttacca gaagtaatta atcctgatgc aacaagaaaa atcacaggta ttctaataga
      661 tggagaaatt ataccgttat ttaaaaatgg aagaaagaaa aataatggag ccaatctaaa
      721 aaaaacgccg ttcaggagta ttaaaaacac atgaatactt tcgaaccagc ttgaaatgtc
      781 catcaaaccc cactcttaat taaaaacatt atttcatgac aaaaagacaa taaaacctca
      841 ccataccatc cagaaagaag tagcaaagat attgagacac ctataagctt tataccaaaa
      901 ggaagtgtct gctcttggag ttgagtcact gtctgtaaca aaccaataga taatccaata
      961 accgtagcta tacctactgg ccataaggaa aagataagga ttaaataaag agccttatta
     1021 cccatataaa ctatatcaga cacattacct ccatcatcta agcaggaaca ttgatatatt
     1081 gttcaatcaa ggccttagac aggatacccc acccatcaag cgcaacaaat aatacaagtt
     1141 ttattggtac tgatattgtg attggactca tcatcatcat accaagtgca agtaaaatgc
     1201 ttgaaataac aagatcaaca acaacaaatg gcaaatataa ataaaaacct attttgaacg
     1261 catctttgat ttcacttagc gcatatgcag gcaagaggga aaacaatgaa taatcattat
     1321 tctctgcact ctttaaatca gcattttctt cttcagacct ttggaaaaaa cgtgctaact
     1381 caagatctgt atgctttttc aagtattgct tatattccat caagccagaa tcagaaaaac
     1441 ggacaatatc agatattgta tcaaattttt gagggccatt tagataattt tcataaccag
     1501 cttcaataat gggtttcatc acgaacaaag ccataatcaa tgcaatacca ttgagagtca
     1561 tatttgatgg aacctgctgc aaaccaagtg catttcgtac cattacaaaa acaatagaaa
     1621 actttatata acaggtacca gcagcaacta aaaatggtaa tagcgtaaaa aaagaaagtg
     1681 tagcgatgag ggacatgtca ctcagcatga gattactcct ttaccatcca agaactaatc
     1741 tcgataccat aaccatcctc aatagaaaca agttccccat gcccaaccaa agaaccattt
     1801 actttaatat ttacatgttt aactatgtca tcgggaaact tgaataattc gttttctaca
     1861 tacattttta gttcattgat tgtcatattt ttttctaaaa gaataaagtc cacttttaca
     1921 ttgatatcat cataattgaa taatgccaaa gaaacttctg tgtgttctga ctcaccatta
     1981 ctttctga
//
LOCUS       DAZSBF010000393         1972 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZSBF010000393 DAZSBF010000000
VERSION     DAZSBF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.399"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="ACHXWD_004373"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="ACHXWD_004373"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(315..758)
                     /locus_tag="ACHXWD_004374"
     CDS             complement(315..758)
                     /locus_tag="ACHXWD_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR7623050.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            915..1844
                     /gene="metA"
                     /locus_tag="ACHXWD_004375"
     CDS             915..1844
                     /gene="metA"
                     /locus_tag="ACHXWD_004375"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR7623051.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          488 a          538 c          454 g          492 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc
      301 acggcagggg cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc
      361 aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc
      421 gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc
      481 tccaccagca tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc
      541 atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc
      601 cacaacggcg cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct
      661 gctgataaaa aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc
      721 ccttcctcat gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct
      781 tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat
      841 tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt
      901 gaggtaatca ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc
      961 gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac
     1021 ttaaggttct gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc
     1081 gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg
     1141 aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc
     1201 aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta
     1261 atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca
     1321 cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc
     1381 ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc
     1441 atgcgcttct gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg
     1501 actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag
     1561 aaggggatgc atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc
     1621 ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag
     1681 acccggatgt accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga
     1741 gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga
     1801 tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg
     1861 atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa
     1921 acgcacctca gcaagtgaat aaattttatt catattgtta tcaataagtt at
//
LOCUS       DAZSBF010000394         1958 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZSBF010000394 DAZSBF010000000
VERSION     DAZSBF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.400"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..518
                     /locus_tag="ACHXWD_004376"
     CDS             <1..518
                     /locus_tag="ACHXWD_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623052.1"
                     /translation="IRIGCMKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIM
                     GFSTQGAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQD
                     NIVALPADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVA
                     RWLAAQKGRRA"
     gene            538..732
                     /locus_tag="ACHXWD_004377"
     CDS             538..732
                     /locus_tag="ACHXWD_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR7623053.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            732..1022
                     /locus_tag="ACHXWD_004378"
     CDS             732..1022
                     /locus_tag="ACHXWD_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR7623054.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            1302..1454
                     /locus_tag="ACHXWD_004379"
     CDS             1302..1454
                     /locus_tag="ACHXWD_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR7623055.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            1466..1831
                     /locus_tag="ACHXWD_004380"
     CDS             1466..1831
                     /locus_tag="ACHXWD_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623056.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT          594 a          438 c          456 g          470 t
ORIGIN      
        1 ttattagaat tggatgtatg aaaagaaaat cattgtcaga caccgacctc aacgccgcca
       61 gaagactgaa agaaatctgg atggcaaaga aatctcagtt gggattgact caggaacgtg
      121 cggcggaaat tatggggttt tcaacacaag gggcggtaag tcactaccta aatggtcaaa
      181 ccccactaaa ccttgaggct gttctaaagt ttgctggctt actgaaagtt ccccctgagt
      241 caatcaggcc agacatggta gatttgctgc aaattgcaag gatgtattgt cgagaaccac
      301 agcaagataa catagtcgca ttaccagcag acacagaaca aacagaggat gaactaccgt
      361 ttgccgtaga gccgatggaa cgagatttaa tccatacgtt cagagctttt ccgaaagaag
      421 atcaagaaca catgcttcag gagatgaaag ataaaaaaga atcaatggat cgtacggttg
      481 cgcggtggtt agctgcacaa aaaggccgtc gcgcctaaat cagaggagat atgtaaaatg
      541 agtacagtcc tttccccaat ggtttcagaa tttgagacag ttgagcaaga aaatagttac
      601 aacgaatggc tgcgggccaa agtagcagca agcctcgcgg atccccgccc ggcgattccg
      661 catgatgagg taatggcgga aatggaacaa attattgccc aaattgctgc tgcaactcac
      721 agaggtgaat aatgttaccc gttttatggc taccatctgc tcgtgatgat ttgcgccaga
      781 tcgtagccta catagctaaa gaaaatcccg cggcagcacg cagaatgaaa attcgaattg
      841 aaacatccgt tttgccgctc acagagcatc cttacctgta tcctccaagc gagcgagttc
      901 ttggtcttcg tgaaattgta actcatccta actacatcat cctttaccga gtaacagcat
      961 caagcataga agtagtaaat attgtgcatt ctcgaagaca gtatccaggc aaaaacagtt
     1021 aattattccc gccgacagaa ccaccttagg gtggtttttt cttgccccaa caaaaaacaa
     1081 tagcgatgct atttacatca aaaaaaataa tgatgctatt gtttcaggcg tcaacccacc
     1141 ccgccccaca gaatgcaggg caatacttcg agttaccaga cagtggtcag gggttaagta
     1201 gccagcccga ggcgtaagaa catgacggca gggttcaatc tttaactatg cagcaggttt
     1261 tttgttccgc caccccggcg ttaaggggaa atgaggtcag catggatact atcgatcttg
     1321 gcaacaacga atctctggtg tgcggcgtgt tccctaacca ggatggaacg tttaccgcca
     1381 tgacgtatac caaaagcaaa acgtttaaaa ccgaatctgg cgcgcgttgc tggttagcca
     1441 gaaacactga ctgatgaggc tgacgatgga atttaaagat ttaccgtctg atgtacagaa
     1501 aacagcagct catacattgc attctgtgct gcgagaaatc gggaaagata ttgcaagcga
     1561 gccagcaaaa gatctggccc ggaaaatcaa gaccgctttc gttgagcttt ataatgttgg
     1621 cactgactct gaaactgtcg agaccaagac cgtaagttca ccaatattct cacttgagcc
     1681 aagcgtatta tcaggtgaaa tatgcaccga gatttccagc gaactgcttc cggtaatacg
     1741 agaagcaatt cgccgtcgcg gtttggatgg aagttatgat catgacgttc tgcaagttct
     1801 caggacaatg gtgacttcac tggggatttg atccctgcgt cctctaagct tttgatatag
     1861 cggtcctcca agaagcggta aattttgtcg aaatcatctt ttccctctgg tttattcaga
     1921 ggattacagg acccgtaagt agcggcatac atttcaac
//
LOCUS       DAZSBF010000395         1949 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZSBF010000395 DAZSBF010000000
VERSION     DAZSBF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.401"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..854
                     /gene="espL1"
                     /locus_tag="ACHXWD_004381"
                     /pseudo
     CDS             1..854
                     /gene="espL1"
                     /locus_tag="ACHXWD_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1026..1133
                     /gene="yniD"
                     /locus_tag="ACHXWD_004382"
     CDS             1026..1133
                     /gene="yniD"
                     /locus_tag="ACHXWD_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR7623057.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1186..1944)
                     /locus_tag="ACHXWD_004383"
     CDS             complement(1186..1944)
                     /locus_tag="ACHXWD_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR7623058.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          596 a          438 c          396 g          519 t
ORIGIN      
        1 acgaatgcca tttgccccta tacatccatc ggcaatgttg ttaataatgg ccacaggcca
       61 gactcaatag cttattacac tattcaaaca gttgcccata ctccctgaaa aagaaatcat
      121 tgaaataata actgcgcaga atagcgttgg tacacctgct ttatttctgg ctatgatgaa
      181 cggacaaact gacaacgtaa aaatatttat gcaagaaatt cagtcactgg tatacaatca
      241 tatcattcat gaagataatc tggttaaatt actgcaaact aaaagtgcta acgaaacacc
      301 tggactctat atctccatgt tgtatggatt cgatgaaata atcgatatct ttctgaatgc
      361 attaaccact ccaatagcac aagaactttt aaacaaaaaa aatggtgatg agtattttag
      421 ccatgaaaat acatgatggt gagccaggat tatacgccgc aatggaaaat aatcaccctt
      481 tgtgtgtcac acggttcctc tctaaaatta atggcatcgc ctttaaatac aagttgagca
      541 aagctaacat catggattta ttaaaaggag ctacagcaca gggaacccct gcattataca
      601 tcgccatgag caagggtaat gaagacgtcg tgttatctta tatatcgacg ctgggtgctt
      661 ttgcaaaaaa acattctttt agtcaacatc agttatttac actattggcc gctaaaaatc
      721 atgacaacat gtcagctgtt catatagcca ttcatcataa gcattataaa actgtagaaa
      781 catattatgc tgctataaat gtcatcagcc aaagcctgag ttttagtgct gatgaaataa
      841 agacgtattt ataaccagct atttacgata tatatcgata cataatttta tttttttcat
      901 aaaacgcaga aacagtgacc atgtgcacaa cgccacccga cggctgtaat aacaaaaaat
      961 cccttgatgc ctatcccttt tgttacactc cgttatcacg cacaagagat atgcaggaca
     1021 ctggtatgcc gactaaacgc tttgataaaa aacactggaa gatggtggtg gtgctactgg
     1081 caatctgtgg cgctatgttg ttgctacgtt gggcagcaat gatttggggc tgagcagtga
     1141 caaacgaaga cagccagacc gcataacacg gtccggcaga taattttaca tgctgtagcc
     1201 cagcgataag gttgtccgac gatcggtatg ttccggtgca gactctggag gttcagagtt
     1261 ccatgtgacg ttgtaagcca ccttcagtcc aaaatgttca ttaatagcaa catttaatgc
     1321 gctttcggag ttcagcgttg tatcttccgc gccaaagacc gaaacaccct gcgtaaattt
     1381 agcgttgtca gtcaactgcc atgcataagc cccggaggca tagcccagcg gctgggtttc
     1441 actggcattg tcggtatatt tgtcgtaacg cacacctggg ccgaattcaa agcggaaact
     1501 gtgtaccggg ccattcaaaa actggcgacc ataacccgcg gtcaacacat cgcgctctcg
     1561 atagccgtta taacggtctg tcagccagct tgcctgacca aataaatagt cataatcagt
     1621 taaattaaaa cggctacgcc cgcccgccgc atatttttct gaagaacgct catcattaga
     1681 agaagtatta ctggcgttcc cccacagcga ccaggcagtg gtttgtccat accaggtcat
     1741 ggtggtgtca gccgtaagtg aggagctttt cgtattgcct gattgtgcaa gatatcctgc
     1801 gttcagatta ccttcgaaag gttttttagc gctggcagga tcgtccatga cagtaaaaac
     1861 ggaatcatcg gcagctgcat tcagtgacgc aaacatgccc cccgccaaca taacgatggc
     1921 aggaactgtc ttcaaaagct tcatttatc
//
LOCUS       DAZSBF010000396         1939 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZSBF010000396 DAZSBF010000000
VERSION     DAZSBF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.402"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHXWD_004384"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHXWD_004384"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR7623059.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHXWD_004385"
     CDS             1506..>1939
                     /gene="yeaK"
                     /locus_tag="ACHXWD_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR7623060.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSV"
BASE COUNT          510 a          451 c          456 g          522 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgt
//
LOCUS       DAZSBF010000397         1923 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZSBF010000397 DAZSBF010000000
VERSION     DAZSBF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.403"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..584)
                     /gene="yeeO"
                     /locus_tag="ACHXWD_004386"
     CDS             complement(<1..584)
                     /gene="yeeO"
                     /locus_tag="ACHXWD_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR7623061.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPL"
     gene            745..820
                     /locus_tag="ACHXWD_004387"
     tRNA            745..820
                     /locus_tag="ACHXWD_004387"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:778..780,aa:Asn,seq:gtt)
     gene            complement(858..1808)
                     /gene="cbl"
                     /locus_tag="ACHXWD_004388"
     CDS             complement(858..1808)
                     /gene="cbl"
                     /locus_tag="ACHXWD_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR7623062.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          541 a          499 c          417 g          466 t
ORIGIN      
        1 aatagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg
       61 gcagctgctg gataactgag taccgtcagc tccagataag tcaacgccag tgctttaact
      121 tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaataagc
      181 gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc
      241 gctcgtcgtc gatcccgctt accgagacta aatgccacaa cgacagtagt accaagatcg
      301 atagcagcaa aaaaagccat aatgaccata ttgaagctgt ccgccaatcc cacgccggcc
      361 atcgcatctt ttcccagcca gctgaccaga aaagtgctca gaacccccat caacaggaca
      421 caggcattct ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact
      481 ttgtagctct tgcgtttagc gtgccagcga gtgccgtgaa caacctggcg taaagcagag
      541 gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa taatgaggga
      601 gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt
      661 gatagaaacg gcaaacagtt ggggaattta aaaatcgggt ttgacaaaag atttttcgcc
      721 gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt
      781 aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt
      841 ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc
      901 tgccgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccagaca
      961 tagttacgct gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga
     1021 tgccgcgtat ccagacggat taaattctct tcctcttgtt cgccgctgga ttgttcggca
     1081 actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgcgca
     1141 cttaatacaa tatctgccag caaacctttg cgggcaaatg cgtcatcaat acgtgagcgc
     1201 cccgtaatcc cctgtcggta agtgattaac ggccacttcg ctattgattc cagcgtcaat
     1261 ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacgaaac
     1321 cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca
     1381 gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg tcccctggat tagctcgagc
     1441 cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc
     1501 tgagtatgcg tcgttgcaat agtgagaacg ccagacgtat cgttggtaaa caggtctgca
     1561 agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg
     1621 cccggttcag tcatgcccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt
     1681 tcatcctcca gttcccgaat atgacggctg acgccggact gtgaggtaaa aagcatattc
     1741 gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg
     1801 aaattcacgg taaactccgg gcagttcaga tttcccgtta ttgttaaagt ctaatgcccg
     1861 gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca
     1921 att
//
LOCUS       DAZSBF010000398         1899 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZSBF010000398 DAZSBF010000000
VERSION     DAZSBF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.404"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(89..643)
                     /locus_tag="ACHXWD_004389"
     CDS             complement(89..643)
                     /locus_tag="ACHXWD_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR7623063.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="ACHXWD_004390"
     CDS             complement(640..930)
                     /locus_tag="ACHXWD_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR7623064.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="ACHXWD_004391"
     CDS             complement(930..1529)
                     /locus_tag="ACHXWD_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR7623065.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="ACHXWD_004392"
     CDS             complement(1601..1813)
                     /locus_tag="ACHXWD_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623066.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAZSBF010000399         1891 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZSBF010000399 DAZSBF010000000
VERSION     DAZSBF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.405"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(50..184)
                     /locus_tag="ACHXWD_004393"
                     /pseudo
     CDS             complement(50..184)
                     /locus_tag="ACHXWD_004393"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1090)
                     /locus_tag="ACHXWD_004394"
     CDS             complement(197..1090)
                     /locus_tag="ACHXWD_004394"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR7623067.1"
                     /translation="MSHIRPDFPEPYGLPVDGLRGKTSIFSHPVYLFLREFSLQDSRG
                     GSLSKKAESLSSGKITIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNESILLNGKTIRKLMKELGISSLIRR
                     KKYRTYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1057..1891
                     /locus_tag="ACHXWD_004395"
                     /pseudo
     CDS             1057..1891
                     /locus_tag="ACHXWD_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_085938460.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="ACHXWD_004395"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          469 a          421 c          458 g          543 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaat tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact
     1861 ggagcaggcg ttgtgggccc gtcgtccgtc t
//
LOCUS       DAZSBF010000400         1871 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZSBF010000400 DAZSBF010000000
VERSION     DAZSBF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.406"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..103
                     /gene="mgtT"
                     /locus_tag="ACHXWD_004396"
     CDS             <1..103
                     /gene="mgtT"
                     /locus_tag="ACHXWD_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR7623068.1"
                     /translation="NGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHXWD_004397"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHXWD_004397"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR7623069.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHXWD_004398"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHXWD_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR7623070.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          535 a          442 c          366 g          528 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg t
//
LOCUS       DAZSBF010000401         1860 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZSBF010000401 DAZSBF010000000
VERSION     DAZSBF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.407"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            200..703
                     /locus_tag="ACHXWD_004399"
     CDS             200..703
                     /locus_tag="ACHXWD_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR7623071.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(782..1033)
                     /gene="yecJ"
                     /locus_tag="ACHXWD_004400"
     CDS             complement(782..1033)
                     /gene="yecJ"
                     /locus_tag="ACHXWD_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR7623072.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1148..1234)
                     /gene="azuC"
                     /locus_tag="ACHXWD_004401"
     CDS             complement(1148..1234)
                     /gene="azuC"
                     /locus_tag="ACHXWD_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR7623073.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1497..1820
                     /locus_tag="ACHXWD_004402"
     CDS             1497..1820
                     /locus_tag="ACHXWD_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR7623074.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          490 a          434 c          409 g          527 t
ORIGIN      
        1 gaaatgtaaa aatatataaa cttgatgatt taagcatttt cttatacccg ttcagacgtt
       61 attcttattt cagatcatcg tcagaattga ctccacgatc acatttcgga ccggcagaaa
      121 ggaattattc tgcaaacagt aattatggtg ttttgattta tcttgcacct ctccacttct
      181 ggatataagg atattaggta tggcaaccgc tggaatgctt ctcaaactca actctcaaat
      241 gaaccgcgag ttttacgcat ccaatctcta ccttcacctg agtaactggt gttctgaaca
      301 gagtctgaac ggcaccgcca ctttccttcg cgcccaggca cagagtaatg tgacccaaat
      361 gatgcgcatg tttaacttta tgaagagtgt cggcgctacc cccatcgtta aagccattga
      421 tgttcccggt gaaaaactga actctctgga agaactgttc caaaaaacga tggaagaata
      481 cgagcaacgt tctagtacgt tggcacagtt agccgatgaa gcgaaagaac tgaatgatga
      541 ttcaaccgtc aatttcctgc gcgatctgga aaaagaacag cagcatgatg gtctgttgct
      601 gcaaaccatt cttgatgaag tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac
      661 cgaccaacat gttctgaatg tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat
      721 gcattgcgcc gatgaaggtt ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt
      781 tttaatggga ttcaccctgt ggggtaaact tgagttcaat aagcgcgatg gctttttgga
      841 ttgcccgcat ggtgaccggg tctgcggcgg cgggatggtt agtaaagtcg atattcttca
      901 gctgactgga cattttttca cgaacttcaa cgggcgcgat tacatcgaga acatccagaa
      961 tttgtttgat aaccaactgg caagcaacca catcagaaac cagttcctga tcggcattca
     1021 gcggctggga catcgtaaac tcctgatagc attttgaaag ccgttatagt agcggcttca
     1081 catcttcagc gatagtcaca tccaccgtca tcaggacaca aaaaaacctg ccggagcagg
     1141 ttttttgtta tcggaacata ttgcctggcg gtacgtcttt gaacgtcttg caatagttat
     1201 tgaacatact tttcaggatt ttgcgcagtt tcatcgcggc actccgacca tttgttatac
     1261 aggtgttatt gtctttgcgc ttataatatg acaaccatca caaaaatcaa tctttatgtg
     1321 atacaaatca cataaatacc cctttaatgt tataaaaatg ataatcaaaa aacagccccc
     1381 ctatttctga cacctacaga tggcaagaaa tagcgcctgc caggcgtctt ttccggccat
     1441 tgtcgcagcg ctgtaacgcg taaaatagtg ctttctctta ctcttctggc tggaccatga
     1501 gacttctgat tctgactctt tcattaataa cgctggcggg gtgtacggtt actcgccaag
     1561 cccatgtgag cgaagttgac gcggcaacag gtattgtacg gttggtttat gaccaggctt
     1621 ttttgcagca tgcccatacc gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc
     1681 agcaggaagg ctatacccac gcggtcccct ttggtcagcc agtaggcaac tgtagccttt
     1741 ttgcgggttc cttatgtttg aatacggaat tcactttgtc atatcagtgc catcattccg
     1801 ccttccctgt ctttctctaa atattttaat cagcgagggg atcttcgctg attaaagaaa
//
LOCUS       DAZSBF010000402         1850 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZSBF010000402 DAZSBF010000000
VERSION     DAZSBF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.408"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(13..285)
                     /locus_tag="ACHXWD_004403"
     CDS             complement(13..285)
                     /locus_tag="ACHXWD_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623075.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            complement(368..892)
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004404"
                     /pseudo
     CDS             complement(368..892)
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            1308..>1850
                     /gene="ospC4"
                     /locus_tag="ACHXWD_004405"
     CDS             1308..>1850
                     /gene="ospC4"
                     /locus_tag="ACHXWD_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFR7623076.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEI"
BASE COUNT          557 a          380 c          396 g          517 t
ORIGIN      
        1 cggggctgaa aatcaccacc acttcgggtc atccgccttc atctccgctt ctgtttcgta
       61 taaatcaaaa cgacggcagg tatggcagaa cgtgacgtat tcgtacggtg aatccgggta
      121 cattttgtgg aaggtttccc agaaacagac cgtgcaggag ggatgaccgg cgataaagtc
      181 tgtaaatacg gactgataag ggtgattatt ggctctggcg acggctttca gaacctcttt
      241 caccattctg gtgtggactt tctggtgctc caggttgtgt gacatgggaa ctcattctgg
      301 atggttactc tgaaagctca tattctgcca caccccgatt tgcagccgcc aggctgccgt
      361 ggttcttacg ccagaaggga tttgtaactg gtctggtcgg ctccggcgta tcagtcagtg
      421 ccaccgggac aggcatgcat tcctgtatgt catcatcgct cagtaagccg tcctcgtact
      481 ggcttttcca tttaaacagc aggttatcat tgatatcgtg ttctctggcg atccgggcaa
      541 caacagcacc aggctgtaat gcctgcttag ccagacggac cttaaattca cggctatagc
      601 tggttcgccg ttcttttcgc catgagcctt ctctgatttg aggctctgtt aattccttct
      661 ttctgttggc ataaaggatg gcgtcaagtt gagcgaatga aactgaatcg ggcaatggcc
      721 atgcgatacc ggatgcaaga aatcgctgaa aaagcgtatg tattgtggaa tgactgagac
      781 ccagacgctg agcgatggcc cggatggtca gtttatcttc aaatcttaaa cgcagggcat
      841 caggcaaata agaacggaag cagggaatat cttttgttgt ctgggaattc atcgttcgtg
      901 tccatcaata tagatgggcg cgattgttgc cagacaggac aattttcaca agacgtcgct
      961 gatggggtgc ttacttataa gaggactcta tcatcgccaa tcatcagaca ttgaaataga
     1021 cgacactgag atgacgctta caattctttt ggtttctgta ataagtaatc tgacatcccc
     1081 cgtgcccaga agaggagagg gatatcaagc ccaagggtta ataattgata ctacggttaa
     1141 aattgccttc agtaaaggaa acgtgattaa attcagggga tttcagagtg aaaaccatac
     1201 aacatattaa aatctgttca tttagctata atacatacaa gaaagtgtcc agttagataa
     1261 tgttatctaa ataaccacag ataaaaacgc acataattgg gggggggatg aaaatacctg
     1321 aagcagtaaa tcatattaat gttcagaata atattgatct tgttgatgga aaaacgaatc
     1381 cgaataaagc aacaaaagca ttacaaaaaa atatattacg cgtaacaaac tcatcttctt
     1441 ctggcataag tgaaaaacat ttagatcact gtgctaatac tgtgaaaaac tttttaagga
     1501 agtctatagc agcgcaaagt tatagcaaaa tgttttctca agggaccagt ttcaaatcac
     1561 taaatctttc tattgaagcg ccatctggag cacggtcatc atttcggtca cttgagcatc
     1621 tcgataaggt gtccagacac tatatctctg agataataca aaaagttcat cctctttctt
     1681 cagacgaacg tcacttactt tctattatta ttaattctaa ttttaacttc agacatcaaa
     1741 gtaactctaa tctgtcaaat aatattctta atattaaatc ctttgataaa attcagtctg
     1801 aaaatataca aacacataag aatacatact ctgaggatat taaagaaatc
//
LOCUS       DAZSBF010000403         1850 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZSBF010000403 DAZSBF010000000
VERSION     DAZSBF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.409"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..74
                     /locus_tag="ACHXWD_004406"
     CDS             <1..74
                     /locus_tag="ACHXWD_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR7623077.1"
                     /translation="RLKGKLTPALLQILIREIKGSSH"
     gene            74..421
                     /gene="tnpB"
                     /locus_tag="ACHXWD_004407"
     CDS             74..421
                     /gene="tnpB"
                     /locus_tag="ACHXWD_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR7623078.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            441..1595
                     /locus_tag="ACHXWD_004408"
                     /pseudo
     CDS             441..1595
                     /locus_tag="ACHXWD_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1599..1758
                     /locus_tag="ACHXWD_004409"
                     /pseudo
     CDS             1599..1758
                     /locus_tag="ACHXWD_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          445 a          488 c          521 g          396 t
ORIGIN      
        1 tcaggcttaa aggtaaactg actccggcgt tattacagat acttatccgc gaaataaaag
       61 ggagcagcca ctgatgatat ctttccctgc aggttcgcgt atctggctgg ttgcaggtat
      121 caccgatatg cgaaatggct ttaacggcct ggcctcaaaa gttcagaacg tcctgaagga
      181 tgacccgttc tccgggcatc tgttcatctt ccgcggacgc cggggtgacc agataaaagt
      241 gttgtgggct gacagtgacg gactgtgcct ctttaccaga cgcctggagc ggggccgctt
      301 cgtctggccg gtcacccgtg acggcaaggt gcaccttact ccggcccagt tatccatgct
      361 tcttgagggc atcgactgga aacacccgaa acgaacggaa cgcgctggca tccgcatata
      421 acccgttgta aagtgaggat atggacacct cacttgctca tgagaatgcc cgcctgcggg
      481 cactgttgca gacgcaacag gacaccatcc gccagatggc gaaatacaac cgcctgctct
      541 cacagcgggt ggcggcttat gcttccgaaa tcaaccggct gaaggcgctg gttgcgaaac
      601 tgcagcgcat gcagttcggt aaaagctcag aaaaacttcg cgcaaaaacc gaacggcaga
      661 tacaggaagc acaggagcga atcagcgcac ttcaggaaga aatggcggaa acgctgggtg
      721 agcaatatga cccggtactg ccatctcccc tgcgccagtc ttcagcccat aaaccgttac
      781 ctgcctcact tccccgtgaa acccgggtca tccgtccgga agaggaatgc tgcccggcct
      841 gtggtggcga actcagttct ctgggatgtg atgtgttaga gcagctggag cttatcagca
      901 gcgcctttaa ggttatcgaa acacaacgtc cgaaactggc ctgttgccgg tgtgaccata
      961 tcgtgcaggc accggtgcct tcaaaaccca ttgcacgcag ttatgccgga gcggggcttc
     1021 tggcccatgt tgtcgctgga aaatatgcag actatctgcc gttataccgc cagtcagaaa
     1081 tataccgtcg tcagggcgtg gagctgagcc gtgccacact ggggcgctgg acaggtgctg
     1141 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
     1201 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
     1261 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa atgcctccgg
     1321 cggtctggtt cgcgtactca ccggaccgga aaggtatcca tccacagaat catctggccg
     1381 gttacagcgg agtgcttcag gccgatgctt acggtagtta ccgggcgttg tacgaatccg
     1441 gcagaataac ggaagcccag cagcgtatcg gtgaactgta tgccatcgag gcagaagtcc
     1501 ggggatgttc agcagaacag cgtctggcgg caagaaaagc cagagccgcg ccactgatgc
     1561 agtcactgta tgactggata cagcaacaga tgaaaattca ttcgctgaaa atggaatgtc
     1621 tccacggaga acactattat ccatcgggaa atagtgcggg caacagtgtt tagttatatc
     1681 gagtgcgatt acaatcgctg gcgacggcac cgttggtgtg gtggtctcag tccggaacaa
     1741 tttgaaaacc agagcctcac ttacgactgt gtccatatta tgtgggtagg atcaataaag
     1801 acgttttata tgtgtctgct tgcagccaga aaatttatac cctgcaggta
//
LOCUS       DAZSBF010000404         1838 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZSBF010000404 DAZSBF010000000
VERSION     DAZSBF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.410"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1087
                     /locus_tag="ACHXWD_004410"
     CDS             join(<1..183,183..1087)
                     /locus_tag="ACHXWD_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
                     /protein_id="HFR7623079.1"
                     /translation="ERIGVAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENA
                     RLKRQLAEQAEELAILPKGRDILCEAPEMKYVFIEKHQAEFSIKAMCRVLRVARSGWY
                     TWCQRRTGISPRQQFRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAA
                     SLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGW
                     LYLAVVIDLWSRAVIGWSMSPRLTAQLACDALQMALWRRKRPRNVIVHSDRGSQYCSA
                     DYQALLKWHNLRGSMSAKGCCYDNACVESFFHSLKVECLHGEHFISREIMRATVFNYI
                     ECDYNRWRRHSWCGGLSPEQFENQNLA"
     gene            1427..1838
                     /locus_tag="ACHXWD_004411"
                     /pseudo
     CDS             1427..1838
                     /locus_tag="ACHXWD_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          453 a          460 c          488 g          437 t
ORIGIN      
        1 ctgaacgcat cggtgttgct gccgcagccc gtgaactcag cctgtatgaa tctcagctct
       61 atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc tgagcgtgaa agtgaactgg
      121 ccgctgaaaa tgcccgcctg aagcggcaac tggcggagca ggcagaggaa ctggctatcc
      181 tccaaaaggc cgcgacatac tttgcgaagc gcctgaaatg aagtatgtct ttattgaaaa
      241 acatcaggct gagttcagca tcaaggcaat gtgccgcgtg ctccgggtgg cccgcagcgg
      301 ctggtatacg tggtgtcagc ggcggacagg gataagccct cgtcagcagt tccgtcagca
      361 ctgcgacagc gttgtcctcg cggcggcctt tacccggtca aaacagcgtt acggtgcacc
      421 acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa ccgtggcggc
      481 aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc cggtcagtta
      541 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
      601 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
      661 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
      721 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
      781 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
      841 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
      901 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
      961 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     1021 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     1081 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     1141 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     1201 gcaaagttat ccacaggcac tgtgcattta aaaaacttat cttttcttct tgattttaaa
     1261 aactcagatc gtcatatcat tatgataaac ataataaaaa actctaaaca tgagaaggtt
     1321 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
     1381 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
     1441 ccttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
     1501 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
     1561 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gctttcaacg gtgccggagt
     1621 tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc gtcatacgga cattaaaaag
     1681 ctcccgcccc ggcggataac atctgcgcca gtcgctcacg ctgatgttgc ccttatctgt
     1741 gaacttgatg ggcagtggag cttcgttggc agcaaggcgc aacaacactg tcacgaacgg
     1801 tgcaatagtg atccacaccc aacgcctgaa atcagatc
//
LOCUS       DAZSBF010000405         1822 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZSBF010000405 DAZSBF010000000
VERSION     DAZSBF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.411"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..224)
                     /locus_tag="ACHXWD_004412"
     CDS             complement(<1..224)
                     /locus_tag="ACHXWD_004412"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623080.1"
                     /translation="MRSRRCRNRGGSVVLENGEQKDARDFEAAKEQCRIDNQLEIACN
                     VLWIVRLPLALYGHHFFRYLSNSITGAGFF"
     gene            1040..1573
                     /locus_tag="ACHXWD_004413"
     CDS             1040..1573
                     /locus_tag="ACHXWD_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFR7623081.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKA
                     GWNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFEGSLKTSNHDLD"
     gene            1586..>1822
                     /locus_tag="ACHXWD_004414"
     CDS             1586..>1822
                     /locus_tag="ACHXWD_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623082.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCE
                     DKEFNPVDIVNEDGKNIMFVVSNNEKIQKTKKSAK"
BASE COUNT          557 a          373 c          437 g          455 t
ORIGIN      
        1 aaaaaaaatc ctgcacctgt aattgagtta gataaatatc ggaaaaagtg atggccatac
       61 agagccagcg gcaggcggac aatccacaga acattgcatg caatttctag ttgattgtct
      121 atcctgcatt gctcttttgc cgcctcaaaa tcccttgcgt ctttttgctc cccgttttcc
      181 agaacgaccg agccgccacg gttccggcag cgccttgagc gcaaccggaa accgtctggg
      241 gagaacccca gacccccatc gctgtaacat cgacggtcta cgaccaccta ccaaccgcgt
      301 ggctgcctat tccgtgcaaa tgggctttgc agggggctga cgcggttcct ggcgacctga
      361 aatcccctgg ttaggggtat cggcgcgcct gacctcctaa aaccctctgt aagcgttttt
      421 cgcgccttcc atgccctgat gggcatttaa aacccgtttc gtgcgtctga gagcttctga
      481 gagcgtttga agagctatct ggcgactcat ccccgaaaga acaacagcgg aatcaccgat
      541 aacatgcgag aggtggcacc gctcgccgca gccccagcga ccgaacgtta gtgagcgagt
      601 gggcgaggaa gcggaagaga accggaaacc acattgagca cgtacgcact gatgcggggc
      661 gttttagcgg tgaacggact gcaaaaacat gatcgagctg ttgaacgtgg agagcgcgaa
      721 atcgggcaaa atcacggctg acgaggcttg cagaagagta agccagtata cactccgcta
      781 tcgctacgtg actgggtcag ggcttcgccc cgacacccca aatgttcgat gaactaatta
      841 aatcttgtgt agcaaaaaag tgctttgata gatctttaga gcaaaaatcg acattctctt
      901 aaaaatcaaa gcattatggc accaacatac gaaacgttat ttgcatgtgt atacgcaaac
      961 atgtttgcgt gtgttgtttc gtaatgtacg atataaattt cgtaaggtac gaaacaacgt
     1021 tgcaggtaag gatgcaagta tgctggaaca gcaatttgaa gatttcgaac agcaaaaacg
     1081 gcgagatcat caggcacaag atcaatccaa aagtgctaag caaaaacgag atcggttgaa
     1141 tccacctgac tggtcgcagg ttactaaggc gggctggaat atgttggact ctatatctga
     1201 gaaggttccc agagcattta ggctatattc tttttttgct caaaatatag ataaatcgtg
     1261 tggggctgtt gtatgtgacc aaaaattctt agctgattat ttcggtgtta gtttaaggac
     1321 gatccaatat tggattggag atttagaaag tattggtgcc atcgtgaaaa taccagtagg
     1381 tatctattat gcttatgcgt tagatccgta tcaggtatgg cgcggatata ataaatctaa
     1441 aaaatatgca gcgtttaacg caaaaacatt aactcaaaaa gatggcgtta ttgatcgcaa
     1501 gttgcggctc atgctctcta ggagagaaga gtttgaaggc tcgcttaaga catctaatca
     1561 tgacttggac taggtatgat gcgctatgag aattaaatat gaatcacgtt ggaaattaga
     1621 agaactaaaa ttagaaatat gtagatcaat agattcgtta attgatgaat atcatatcga
     1681 atatgcaatg ggaataaatc tatatatgaa ttgtgaagat aaggagttta atcccgttga
     1741 tattgtcaat gaagatggaa agaatattat gtttgttgtt agcaacaatg aaaaaataca
     1801 aaaaacaaaa aaaagtgcga ag
//
LOCUS       DAZSBF010000406         1816 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZSBF010000406 DAZSBF010000000
VERSION     DAZSBF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.412"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHXWD_004415"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="ACHXWD_004415"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR7623083.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1006..1416
                     /locus_tag="ACHXWD_004416"
     CDS             1006..1416
                     /locus_tag="ACHXWD_004416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623084.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(1450..>1816)
                     /locus_tag="ACHXWD_004417"
     CDS             complement(1450..>1816)
                     /locus_tag="ACHXWD_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR7623085.1"
                     /translation="MMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVY
                     SKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFY
                     DNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          369 c          444 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcatt
//
LOCUS       DAZSBF010000407         1709 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZSBF010000407 DAZSBF010000000
VERSION     DAZSBF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.414"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..483
                     /gene="cas6e"
                     /locus_tag="ACHXWD_004418"
     CDS             <1..483
                     /gene="cas6e"
                     /locus_tag="ACHXWD_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR7623086.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
     gene            480..1403
                     /gene="cas1e"
                     /locus_tag="ACHXWD_004419"
                     /pseudo
     CDS             480..1403
                     /gene="cas1e"
                     /locus_tag="ACHXWD_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            1400..1693
                     /gene="cas2e"
                     /locus_tag="ACHXWD_004420"
     CDS             1400..1693
                     /gene="cas2e"
                     /locus_tag="ACHXWD_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR7623087.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT          404 a          395 c          494 g          416 t
ORIGIN      
        1 cgcttttttg tcctgtcgca ggaacgcccg gcggaaagtg atattttcac catcgaatgc
       61 cgaccatttg cgccagagtt gcgtaccgga caatcccttt gtttcaacct gcgggcgaat
      121 ccaacaattt gtaaagcggg caaacgccac gacttgctga tggaggcgaa acggcaggtg
      181 agggggcagg tggaaggaag tgatgtctgg ttgcatcaac aacaggctgc gctggactgg
      241 ctggcagcgc agggggagag aagcggtttt acactgctgg atacttcggt tgatgcctac
      301 cgacaacagc aactgcggcg ggaaaactcc cggcaactga tccagttcag tagcgtggat
      361 tatacgggga tgcttacggt caccgctcct gggttatttc tgcaacgtct cagtcagggg
      421 tatggcaaaa gccgggcgtt cggttgtggt ctgatgctga tcaaacccgg agcagaggcg
      481 tgacgtttgt accgctgagc ccgatcccat taaaagatcg cacttccatg atcttcctcc
      541 agtacggtca aatcgacgta ctggacggcg ctttcgtgct gatcgacaaa accgggatcc
      601 gcacgcatat tccagtggga tcggtcgcct gcattatgct cgaaccggga acaagagttt
      661 cccacgcggc agtccatctg gccgccacgg tgggaacact gctggtctgg gtcggtgaag
      721 cgggcgttcg cgtttactcc tccggacaac ccggaggggc gcgggcagat aaattactct
      781 actaggcaaa gctggcttta acggaagatc tacggttgaa ggtggtgcgc aaaatgtatg
      841 aattacgttt tcgtgaacca ccgccagctc gccgttcagt ggagcagtta cgtggaattg
      901 agggatctcg cgttcgccag acgtatgcat tactggcgaa acaatatggt gtgaaatgga
      961 atggtcgcaa atacgatcct aaagactggg aaaaaggcga tgttgtgaat cgctgcatca
     1021 gtgctgctac atcatgtctg tacggtatat ctgaagcggc agtattagcc gcgggatatg
     1081 cgcccgctat tgggtttatc catagtggca aaccgctttc atttgtttat gacatagccg
     1141 atatcattaa atttgattcg gttgtgccaa aggcatttga aatagcagcg aggcaacccg
     1201 cagaacctga taaagaagtc agattagcct gtcgcgatat tttccgtagc actaagttaa
     1261 cgggcaaatt aataccgtta attgaggaag tccttgctgc aggtgaaatt gaaccaccac
     1321 aacccgcgcc ggatatgtta ccgcctgcca tccctgaacc tgaaacgctg ggtgatagtg
     1381 gtcaccgggg gcgcggcgga tgagcatggt cgtggttgtt acagaaaatg tccctccgcg
     1441 cttacgtgga cggctcgcaa tctggctact ggaagtgcgt gccggtgtgt atgttggtga
     1501 tacatcaaaa cgtattcggg agatgatctg gcagcaaatt acccaactgg ctggttgcgg
     1561 aaatgtggtg atggcctggg cgaccaatat cgagtcgggt tttgaatttc agacctgggg
     1621 agaaaacaga cgtattccgg tggatttgga tgggttacgt ttggtttctt ttcttcctgt
     1681 tgataatcaa taagttatcc gttctttaa
//
LOCUS       DAZSBF010000408         1693 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZSBF010000408 DAZSBF010000000
VERSION     DAZSBF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.415"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..799
                     /locus_tag="ACHXWD_004421"
                     /pseudo
     CDS             1..799
                     /locus_tag="ACHXWD_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="ACHXWD_004422"
                     /pseudo
     CDS             914..1255
                     /locus_tag="ACHXWD_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1213..1693
                     /locus_tag="ACHXWD_004423"
                     /pseudo
     CDS             1213..1693
                     /locus_tag="ACHXWD_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          424 a          409 c          499 g          361 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggccagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtcaggc ccggatgttc atcagttaca gcgaagtcag cataaaaaat
     1261 cccgacaaca gcggccaggc gctcccgctc acgtatgtct gttgccggga gcaggcagag
     1321 gacggtgcct gctggcatct gctgacgtca ggaaaagcgg cgagtgccgc cgatgcacga
     1381 cgtattgtca gccattacga gcgacgctgg ctgacagagg aataccacaa ggcgtggaaa
     1441 agtggtggta catggaatcg ctgcgaatgc agacccggga taaccttgag cgcatggtgg
     1501 ttatccaggc gtttatagcg gtacgggtgc tgggcctgcg gcagggaggt gtcagtgaag
     1561 aaacacaaaa tgacagctgt gagaaaatac tgacgccgac agagtggaaa ctactgtggg
     1621 ttaagctgga aggaaagccg cttccggtgc aggcaccgac cctgaaatgg gcctggggcg
     1681 atggcatgac agc
//
LOCUS       DAZSBF010000409         1688 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZSBF010000409 DAZSBF010000000
VERSION     DAZSBF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.416"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..303)
                     /locus_tag="ACHXWD_004424"
     CDS             complement(<1..303)
                     /locus_tag="ACHXWD_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFR7623088.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCITLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYIL"
     gene            432..779
                     /locus_tag="ACHXWD_004425"
     CDS             432..779
                     /locus_tag="ACHXWD_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR7623089.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(799..1389)
                     /locus_tag="ACHXWD_004426"
     CDS             complement(799..1389)
                     /locus_tag="ACHXWD_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623090.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(1386..1646)
                     /locus_tag="ACHXWD_004427"
     CDS             complement(1386..1646)
                     /locus_tag="ACHXWD_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623091.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
BASE COUNT          385 a          470 c          418 g          415 t
ORIGIN      
        1 aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg tataaacagc
       61 aagcaatccc atttttccag gtgtatcagc aaaaaaaacg ctgttccaga aatcaggccg
      121 ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca aaaccattcc
      181 gcagagagta atacaccaga acagtaatct gtatttaaaa tcataatccc atgacatata
      241 cagcatatat cctcccgtca cccatcccca ccacatatta ttaaaataat attttctgtt
      301 catctgccag ttttcctttc ttttgcactt ttgtcagtgt actgatgcat gacaacataa
      361 ccacagcatg ataataataa tcaataacaa taagctgtgt cacgtttaca tcatggaacg
      421 gaggtgctcc tgtgactttt gagcagaaaa aagcccgggc cattgccctg atggacagca
      481 aaaagatgtg gcggagcaat tatgcccctc ctcttctgcg tattctctgg cggctgggta
      541 tccggcttcc gccattaccc tttatgccgt tctggcaggt cacggtgctg acggggggct
      601 tatggggcat ttcctgggga tgtgcaatgt ggtttatcta ctgggggccg tcaggaatgg
      661 tggcaggtga ggccattatt ataagtatca cggggggttt tttgtccgga ctgctcatgg
      721 cctctttcca ctggtggcgc aggaaagtta accgactgcc accatgggat gatgtgtaaa
      781 ccgtgcacag gcgtgttctt acggagcggc actttcattt atctgtacgc gaacctccat
      841 tcctgcatcg tgacaggccg gcagccactg cgccacttcc agcggatcgc cctcccggcg
      901 taccacccag ccttctttat tccataactg cagacaggtg ctgccgtcga gacgcaccac
      961 aaaatcccca cggcaggcct gatacgttat gccgttcagc tccaccgcca gatcagtggc
     1021 agaagtgccg cctgcgggca gacctgctgc cgggaccagc ggcagtacga cgacttctgc
     1081 cgcacgctgt tctgcccgga tgcggtcctg ttctgccagc agcagaggct gtgccacggc
     1141 ccggccggcg tccgtcagct ccactgccag ctgcaggttc ggagcgcgca gggtacgcag
     1201 ccagccggca tcctccagac gacggcagga ggcgcgcagg tttggcccgt agaccggggc
     1261 ttccccgctt tgctccagca cccgttctat gtcccgcgtc agcacgggtc ctggccgttt
     1321 ttcatcaagg gcagccagca caatcagcac tctccgctgc agtggcgacg gtcggcggct
     1381 ctgagtcatt tccagtcatc ctccggccag atgagctttc tgaggcgctg atggtcggcg
     1441 tcaatgatct cctgtctctt tatggcattc attgaggtat agccatttat ggccagttca
     1501 tgccagaaga tcagcacggc tccggctttg gctgactcgc agagataagt tacgccgtgg
     1561 ctgtccagat tgcattcccg gatgcgtttt tccgcaaacc gggtcagttg ttcatagctg
     1621 agtgaaaacg tcatttcctg atccatcctg cacgttccat ctgataatgt tctgacaggt
     1681 aaaaatca
//
LOCUS       DAZSBF010000410         1683 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZSBF010000410 DAZSBF010000000
VERSION     DAZSBF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.417"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..764
                     /gene="spa40"
                     /locus_tag="ACHXWD_004428"
                     /gene_synonym="spaS"
     CDS             <1..764
                     /gene="spa40"
                     /locus_tag="ACHXWD_004428"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFR7623092.1"
                     /translation="LSAVLPTLVQTKFVLATKAIKIDFSVLNPVKGLKKIFSIKTIKE
                     FFKSILLLIILALTTYFFWINDRKIIFSQVFSSVDGLYLIWGGLFKDIILFFLAFSIF
                     VIILDFVIEFILYMKDMMMDKQEIKREYIEQEGHFETKSRRRELHIEILSEQTKSDIR
                     NSKLVVMNPTHIAIGIYFNPEIAPAPFISLIETNQCALAVRKYANEVGIPTVRDVKLA
                     RKLYKTHTKYSFVDFEHLDEVLRLIVWLEQVENTH"
     gene            786..1019
                     /locus_tag="ACHXWD_004429"
     CDS             786..1019
                     /locus_tag="ACHXWD_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFR7623093.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT          545 a          247 c          314 g          577 t
ORIGIN      
        1 ttctttcggc tgtgttgcca acattggttc aaacaaagtt tgttcttgcg actaaagcta
       61 tcaagattga tttttcagta ttaaaccctg ttaaagggtt aaaaaaaata tttagtataa
      121 agacaataaa agaatttttc aaaagcattc tgcttcttat tattctagca ttaacaacct
      181 atttcttttg gattaatgac cgaaaaataa ttttttctca ggtgttttct agtgttgatg
      241 gcttatatct tatttggggg gggctgttta aggatataat attatttttc ttggcatttt
      301 ctatttttgt tattattctt gactttgtga ttgagttcat tttatacatg aaagatatga
      361 tgatggataa acaggagata aaaagagaat atatagagca agagggacac tttgagacaa
      421 agtcgagaag gcgtgagttg catatcgaga ttctttcaga gcagactaaa tctgatatac
      481 gtaattcaaa attagtggta atgaacccga ctcatattgc aattggtatt tattttaatc
      541 cagaaatagc gcctgcacct tttatttctc tcattgaaac taaccagtgt gccttggctg
      601 tcagaaaata tgcaaatgaa gttggtatac caactgtgcg tgatgtgaaa ttagctagaa
      661 aactatataa aacacataca aaatatagtt ttgttgattt tgaacacttg gatgaagtcc
      721 tacgtcttat tgtttggctt gagcaggttg aaaacactca ttaataagta aaggagatgt
      781 gttatatggg agttaatttc tgtaataaaa taggtattga tcagtctgaa tttgaaatag
      841 aatcttccat cattaactcc attgctaatg aggtattgaa cccaatatct ttccttagca
      901 ataaggatat aataaatgtt ttactcagaa agatttcatc tgaatgtgac ctggttagaa
      961 aagacattta tcgttgtgct ctggagttgg tcgttgaaaa aacacctgat gatttataat
     1021 cattcataat aactcttttg ctgatataaa tatttttgtt gaagatattt atgtagtcac
     1081 aggatcaatt gtagaaaatt gttatgtttt tgttaggaac tgcactaaat aatatatgat
     1141 aagacagcaa aaacgactga ccataatcct tcttttatta ggagttgata agagagacta
     1201 ttcatcctgt aatgttaaaa ctctgttata tagtattcgc gattatgcaa agtctgtaaa
     1261 tgatcatgag atactaaccg agagtaatcg tttattatct cattgcattt cagatagtaa
     1321 cggagctttc tttaaaagta gtaaatacgt tccccttaag tacttacgta aaaggagaat
     1381 agcacgaaag ataccaaatg attagcactt tgctagtaag cttgctggta aactatattg
     1441 accgttgggt ttgttttgag actgtaattt aaattgtgta attgcctgtt tttgatatgt
     1501 ttactccaac aacggagaca ggtaaattat ggacgaaaag aagcttaaag cccttgcggc
     1561 tgaactggct aaaggcctca aaactgaggc cgacctcaat cagttttccc gtatgctgac
     1621 gaaatatccc ggctcttaaa acgatagtgg ctcaggcttt catttttcag cgttccccag
     1681 aag
//
LOCUS       DAZSBF010000411         1681 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZSBF010000411 DAZSBF010000000
VERSION     DAZSBF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.418"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            122..376
                     /gene="bssS"
                     /locus_tag="ACHXWD_004430"
     CDS             122..376
                     /gene="bssS"
                     /locus_tag="ACHXWD_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR7623094.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            491..1609
                     /gene="solA"
                     /locus_tag="ACHXWD_004431"
     CDS             491..1609
                     /gene="solA"
                     /locus_tag="ACHXWD_004431"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR7623095.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          433 a          418 c          414 g          416 t
ORIGIN      
        1 ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt
       61 cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat
      121 gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac
      181 cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga
      241 gcaggaaggg actgaagttg gtcaaacgct ctggttaacc actgatgttg ccagacaatt
      301 tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc aggtaaacga
      361 gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt
      421 accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag
      481 agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc
      541 cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc
      601 gcatcaacac ggcagccacc acggcgatac gcgattaatt cgccatgctt atggtgaagg
      661 cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca
      721 caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc
      781 cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct
      841 cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg
      901 cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca
      961 actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca
     1021 tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat
     1081 tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg
     1141 taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc
     1201 gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc
     1261 gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc
     1321 gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca atgtattgcc
     1381 gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga
     1441 ttttattatc gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg
     1501 gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacaa
     1561 aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg
     1621 gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc
     1681 a
//
LOCUS       DAZSBF010000412         1678 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZSBF010000412 DAZSBF010000000
VERSION     DAZSBF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.419"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..286
                     /gene="traL"
                     /locus_tag="ACHXWD_004432"
     CDS             <1..286
                     /gene="traL"
                     /locus_tag="ACHXWD_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR7623096.1"
                     /translation="KYRFPETLTNQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGA
                     AVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
     gene            308..490
                     /locus_tag="ACHXWD_004433"
                     /pseudo
     CDS             308..490
                     /locus_tag="ACHXWD_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            492..1678
                     /locus_tag="ACHXWD_004434"
                     /pseudo
     CDS             492..1678
                     /locus_tag="ACHXWD_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
BASE COUNT          388 a          419 c          463 g          408 t
ORIGIN      
        1 gaaatatcgt ttcccggaaa cactgaccaa ccagagccgc tggtttggcc tgccactgga
       61 tgaactgatc cccgcagcaa tctgtgttgg ctggggtatc acaacatcga aatatctgtt
      121 cggtattggt gcagcggttc tggtttattt cgggattaaa aaactgaaaa aagggcgggg
      181 cagttcctgg ttacgtgacc tgatttactg gtatatgcca acagccctgc tgcgcggtat
      241 ttttcataat gttcccgatt cgtgtttccg gcagtggatt aaatagaact gataccagga
      301 ttgttatatg gaacacggtg cccgtttaag taccagtcgt gtaatggcca tcgcctttat
      361 atttatgtca gtgcttattg ttctcagcct ctctgttaac ctcattcagg gggtgaataa
      421 ctaccgtctt cagaatgagc aacgcactgc cgtgacccca atgggattta atgtgccctt
      481 tgccgtgtca cacctaccgt aaccagctgg cgtttatcga tattttcttc cggggaatga
      541 ataacaccaa ctacaacatg gcggtctgtg gcacctccgg ggccggtaaa accgggctga
      601 tacagccact tatccgcagc gtgctggact ccgggggctt tgccgtggtg ttcgacatgg
      661 gggatggcta caagtccctg tgtgagaaca tgggtggcgt atatctggac ggtgaaaccc
      721 tgcgttttaa cccgtttgcg aacatcaccg atatcgacca gtcagcggag cgtgtccgtg
      781 accagttgtc agtgatggcc agccccaacg gtaacctgga tgaagtgcat gaaggtctgc
      841 tgctgcaggc ggtcagggcc tcctggctgg ccaaagagaa cagggcacgt attgatgacg
      901 tggtggattt cctgaaaaac gccagtgaca gcgagcagta tgccgggtca ccgactatcc
      961 gcagccgtct ggacgaaatg attgtgctgc ttgaccagta cactgccaac ggcacttacg
     1021 gccagtattt taactctgat gagccgtccc tgcgggatga cgcaaaaatg gtggtgctgg
     1081 agctgggcgg actggaagac cgtccgtcac tgctggttgc ggtgatgttc tccctgatta
     1141 tctacatcga gaacaggatg taccgcacgc cgcgtaacct caagaaactg aacgtcattg
     1201 atgaaggctg gcgtctgctg gacttcaaaa atcacaaggt cggtgagttt attgagaaag
     1261 gttaccgtac cgcccgtcgt cataccggtg cttatatcac catcacacag aacatcgtcg
     1321 actttgactc tgacaaggcc tccagtgcgg cccgtgcggc atggggtaac tcctcctaca
     1381 aaattatcct caaacagagt gcgaaggagt tcgcaaaata caaccagctg tatccggacc
     1441 agttccagcc actgcagcgc gacatgattg gtaagtttgg tgcggccaaa gaccagtggt
     1501 tcagttcctt cctgctgcag gtggaaaacc attcctcctg gcaccgcctg tttgtggacc
     1561 cgttaagccg cgccatgtac agctctgacg gcccggattt tgagtttgtg cagcagaaac
     1621 gtaaagaggg gctgagtatt catgaggcgg tgtggcagct ggcgtggaag aagtcagg
//
LOCUS       DAZSBF010000413         1676 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZSBF010000413 DAZSBF010000000
VERSION     DAZSBF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.420"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..889)
                     /locus_tag="ACHXWD_004435"
     CDS             complement(<1..889)
                     /locus_tag="ACHXWD_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7623097.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
     gene            complement(989..1345)
                     /locus_tag="ACHXWD_004436"
     CDS             complement(989..1345)
                     /locus_tag="ACHXWD_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR7623098.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(1481..1588)
                     /gene="rprA"
                     /locus_tag="ACHXWD_004437"
     ncRNA           complement(1481..1588)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHXWD_004437"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT          538 a          333 c          392 g          413 t
ORIGIN      
        1 tgggatgcag gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta
       61 atgttgttgg gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg
      121 aacccacggt gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa
      181 attgtccgta ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc
      241 catataaggt ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg
      301 ttttctttat gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa
      361 acagagcgtt aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt
      421 cagcccacac taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg
      481 ctttaattaa aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat
      541 aagtgcctgc atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga
      601 taatgatgtt attggtctgc aggatgccaa aaaagaaggc catatagcag cacatcccga
      661 gcatgataaa agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac
      721 ttaatcgacc aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc
      781 aaagtgtctc cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca
      841 ggtaattaaa atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt
      901 agcaaaggtg ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta
      961 acaacgacaa ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata
     1021 attcagcggc taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt
     1081 gttgaaacgc ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta
     1141 ttttctctat gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct
     1201 cgactacaca atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt
     1261 caccggtctg ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga
     1321 gtgcctggag ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat
     1381 aaataacatt atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta
     1441 ttggaaagag tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg
     1501 ggagatgggc aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt
     1561 ataaatcaat atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt
     1621 cgatcagatt cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactac
//
LOCUS       DAZSBF010000414         1665 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZSBF010000414 DAZSBF010000000
VERSION     DAZSBF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.421"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHXWD_004438"
     CDS             complement(<1..105)
                     /locus_tag="ACHXWD_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623099.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGK"
     gene            complement(183..604)
                     /locus_tag="ACHXWD_004439"
                     /pseudo
     CDS             complement(183..604)
                     /locus_tag="ACHXWD_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            complement(588..815)
                     /locus_tag="ACHXWD_004440"
     CDS             complement(588..815)
                     /locus_tag="ACHXWD_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR7623100.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYAPKVYDEYRKAKRPWKVIHENQA"
     gene            892..1299
                     /locus_tag="ACHXWD_004441"
     CDS             892..1299
                     /locus_tag="ACHXWD_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623101.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1503..1658
                     /locus_tag="ACHXWD_004442"
     CDS             1503..1658
                     /locus_tag="ACHXWD_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR7623102.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          469 a          436 c          343 g          417 t
ORIGIN      
        1 cttccctaat gcggcatctg gatcaatatt cagaatctca gcaagcctga atatttccgg
       61 cttatccggc gtaataacct cgagctttat ccagtttgaa gccatttgtt ttcaccttgt
      121 aacgctcgca gcgttacatt taactgatac cgaacaaaac aatccggcac gattaatttc
      181 aatcaatgca ctacgacaga atcgccgggc gacccaccac cgctgaaatg tgctttccgg
      241 taaacggcct ggacagcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc
      301 gccagtaatg cacggcgttc cagggtatcg aagatacaga gtcggtgacg cagctcacgc
      361 ggaagaattg ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt
      421 tcacctttca accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtacc
      481 agagacaatt cgccgccccc ctgccgcaga tattcttcag taattgcgtt agcgacccac
      541 gcctgccctt tttcggctgc cagggcaaca gcactgattc gatgtgctca tgcctgattt
      601 tcatgaatca ccttccaagg tcgttttgcc ttacgatatt cgtcataaac tttgggggcg
      661 tactgaagtt ccccgccaga tgcctcttgc aggcgcatcg cgcgaccttc aggaaccagt
      721 tccccccatg cagcaatgct tgccagccta actcctgcgg cattggcaag ctttgttttg
      781 ctgccaaaaa acgctatagc atcaattttc aacatatcga gctccttaga ttttcctaag
      841 gaaactagat cgtagagaaa cctaagtcaa gaaaaattag aattccctaa tatgaaaaac
      901 gaaaccttcg gtgctcgcct cttacatagg cgtaaaaaat taaaactgtc tcaggccgca
      961 ttaggtaagc tggtcaaagt ggctcacgta acaatttctc aatgggaaag agatgaaaca
     1021 cagccggcgg ggaaaagatt attcgcactg agccaagcgc ttcagtgctc accgacttgg
     1081 cttctttttg gggatgaaga taaacaacca ggcgaaccga tcccggataa tcagccagcc
     1141 attctgacag aagatcaaaa agagttactt caactgttcg acgcactgcc tgagtcagag
     1201 caaaaggccc tgttgtcaga gatgcgtgct cgagttgaaa atttcaacaa actttttgaa
     1261 gaactactca aagctcgcaa aagaagcgca aacaaataat cccctttttt ctccacaccc
     1321 tgtaataaaa agcacaaact ttcaaacact tgtgtttttt acatcaaaaa aacttaggtt
     1381 tttctacaca aaaatcttga ccgcccacct taggttattc taaatttcat tcatcaagac
     1441 accgcacggt gttctcagca aacagttccg ctaccccggc gttaagggga aatggggtca
     1501 acatgaatac tatcgatctt ggcaacaacg aatctctggt gtacggcgtg ttccccaacc
     1561 aggacggcac attcaccgcc atgacgtata ccaaaagcaa aacgtttaaa accgaagctg
     1621 gcgcacgtcg ttggttggga agacattcag gtgagtaaaa tgaac
//
LOCUS       DAZSBF010000415         1654 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZSBF010000415 DAZSBF010000000
VERSION     DAZSBF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.422"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..334
                     /gene="spa15"
                     /locus_tag="ACHXWD_004443"
                     /gene_synonym="spaK"
     CDS             <1..334
                     /gene="spa15"
                     /locus_tag="ACHXWD_004443"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFR7623103.1"
                     /translation="ITDLDSHSAITISLDSMPAINIALVNEQVMLWANFDAPSDVKLQ
                     SSAYNILNLMLMNFSYSINELVELHRSDEYLQLRVVIKDDYVHDGIVFAEILHEFYQR
                     MEILNEVL"
     gene            338..1630
                     /gene="spaL"
                     /locus_tag="ACHXWD_004444"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             338..1630
                     /gene="spaL"
                     /locus_tag="ACHXWD_004444"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFR7623104.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKNRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
BASE COUNT          496 a          252 c          372 g          534 t
ORIGIN      
        1 tattacagat ttagacagcc attcagcgat taccatttct ttggatagta tgcctgctat
       61 taatatagct cttgtcaatg agcaggttat gttatgggct aattttgatg caccaagtga
      121 tgtaaaactt cagtcatctg cttataacat attaaattta atgttaatga actttagtta
      181 tagcattaat gagctggtag agcttcatcg ttctgatgag tacttacagt taagagttgt
      241 tattaaagat gactacgttc acgatgggat tgtttttgca gagattctgc atgagtttta
      301 tcagaggatg gaaatcttaa atgaggtctt ataatcaatg agctatacaa aattgctcac
      361 tcaattatct tttcctaata gaatctcggg gccaatcttg gaaacaagtc ttagcgatgt
      421 ttcgattggt gagatttgta acattcaggc tggaattgaa agtaatgaaa ttgttgcaag
      481 agctcaggtt gtaggatttc atgatgaaaa aacaatatta agcttgattg gaaattctcg
      541 tggactttca cggcaaacgt tgattaagcc cactgcccag tttcttcata cgcaagtcgg
      601 ccgtggatta ttgggagcag tagtcaatcc tttaggggag gttactgata agtttgctgt
      661 tacggataac agtgaaattc tttatcgacc tgtagataat gctcctccgc tatatagtga
      721 aagggctgca attgagaagc cttttttaac aggtattaag gttattgatt ctttactcac
      781 gtgtggtgaa ggacagcgaa tggggatttt tgcgtcagct ggttgtggca aaacttttct
      841 catgaatatg ctcattgaac atagtggtgc tgatatatat gttattgggt taattggtga
      901 gcgaggtcga gaggttacag aaacggttga ttatttgaaa aactctgaga aaaaaaacag
      961 gtgtgtttta gtatatgcaa cttcggatta ctcttcggtt gatcgttgta atgctgcata
     1021 tatagccact gctatagccg aattttttag gactgaagga cataaagtag cgctttttat
     1081 tgattcatta acaaggtatg ccagagcatt acgtgatgtg gccttagccg ctggagaatc
     1141 acctgccaga agaggctatc cggtttcggt ttttgatagc ttacccagac ttcttgaaag
     1201 gccaggaaag ttaaaggcag gtggctctat tactgcattt tacactgttc ttttggagga
     1261 tgatgatttt gctgatccat tagctgaaga ggtaagatcc attttagatg gacatatata
     1321 tttgagcaga aatctagccc aaaaaggaca atttcctgca attgattcct taaaaagtat
     1381 aagcagggta tttacacagg ttgttgatga aaaacatcgt attatggccg ctgcatttcg
     1441 ggagctactt tctgaaatag aagagctaag gacaattatt gactttggtg aatacaaacc
     1501 gggggagaat gcctctcagg ataaaatcta caacaaaata tctgttgttg agagttttct
     1561 gaagcaagat tatcgactcg gttttactta tgagcagaca atggagctta ttggtgaaac
     1621 aattagataa ggttttaaaa atcaaagata aata
//
LOCUS       DAZSBF010000416         1639 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZSBF010000416 DAZSBF010000000
VERSION     DAZSBF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.423"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            181..624
                     /gene="cmtB"
                     /locus_tag="ACHXWD_004445"
     CDS             181..624
                     /gene="cmtB"
                     /locus_tag="ACHXWD_004445"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR7623105.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            652..>1639
                     /gene="cmtA"
                     /locus_tag="ACHXWD_004446"
     CDS             652..>1639
                     /gene="cmtA"
                     /locus_tag="ACHXWD_004446"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR7623106.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          390 a          359 c          402 g          488 t
ORIGIN      
        1 atgcatcacg atatgcgtat tgacatttgt tgttatatct ataactcaat gttatataag
       61 aaattaataa ttcactgttt tcaaaacacc ggtttccctg ctcaattgct ttcattaaac
      121 cgcacgagta ttttattttt accgccggac ggtaccctaa aaataaaata aggataattt
      181 atgcggctta gtgactattt tccagaatca tcaatctcag ttatacattc agcaaaagat
      241 tggcaggaag ctatcgattt ctcgatggta tcattgctgg ataaaaacta tatcagcgag
      301 aattacattc aggcaattaa agattccacc attaacaatg gcccttatta tattctcgca
      361 ccaggcgtgg caatgcctca tgcgcgaccg gaatgtgggg cgcttaaaac cgggatgtct
      421 ttgacattac ttgaacaagg tgtttatttt ccggggaatg acgaaccaat aaaattactc
      481 atcggccttt ctgctgccga tgccgattcg cacattggcg ctattcaggc gttaagtgaa
      541 ttactgtgcg aagaagaaat actcgaacaa ctcttaacag cagcatcaga aaaacaatta
      601 gcggacataa tcagccgcgg ataattactc tctcctctac aggataatat tatggaaaac
      661 aagtctgctc gtgcaaaggt ccaggctttt gggggctttt tgacggcaat ggtcatcccc
      721 aatattggtg cttttattgc ctggggtttt attactgcgt tatttattcc caccggttgg
      781 ctgcctaacg aacatttcgc caaaattgtc ggcccgatga ttacctattt attgcccgtg
      841 atgattggtt ctaccggtgg tcatctggtc ggcggtaaac gcggcgcggt catgggcgga
      901 ataggtacta ttggtgtgat cgtcggcgca gagatcccga tgttccttgg ctcaatgatt
      961 atggggccgc tcggtgggct ggtcataaaa tatgttgata aggcactgga aaaacgcata
     1021 cctgccggtt ttgagatggt tatcaataac ttctcattag gtatcgcggg gatgctcctt
     1081 tgtctgctgg gttttgaagt tatcggcccg gcggtgttaa ttgccaatac tctcgtcaaa
     1141 gagtgtattg aggcgctggt acatgcgggt tatctgcctc tgctgtcagt catcaatgaa
     1201 ccggcgaaag tgcttttcct caataatgcg atcgatcagg gcgtctatta tccgctggga
     1261 atgcaacagg cttcggttaa cggtaaatcc atcttcttta tggtggcgtc caatcctggg
     1321 cctggcttag ggctgctgct ggcgtttacc ttgtttggta aagggatgag taaacgttct
     1381 gcgcccgggg cgatgattat tcacttcctc ggtgggatcc acgaactgta tttcccgtat
     1441 gtgctgatga agccgctgac cattattgcc atgattgcgg gcggtatgtc tggcacctgg
     1501 gtgtttaact tactggacgg tggtctggtg gctggcccaa gtccggggtc tatctttgct
     1561 tacctggcac tgacgccgaa aggctcgttc ctggcgacaa ttgccggtgt tacggtagct
     1621 accctggtgt cctttgcta
//
LOCUS       DAZSBF010000417         1618 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZSBF010000417 DAZSBF010000000
VERSION     DAZSBF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.424"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..202)
                     /locus_tag="ACHXWD_004447"
     CDS             complement(<1..202)
                     /locus_tag="ACHXWD_004447"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR7623107.1"
                     /translation="MAEPMAQRPICRQNSHRLHDRLGEPQYTDSLHSQGSASSMWMRH
                     VGGHERFRTGDGQLNTQANRYVL"
     gene            complement(162..1618)
                     /locus_tag="ACHXWD_004448"
                     /pseudo
     CDS             complement(162..1618)
                     /locus_tag="ACHXWD_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
BASE COUNT          364 a          464 c          362 g          428 t
ORIGIN      
        1 gcaatacata gcggttagcc tgagtattta gctggccgtc accggtcctg aaacgttcgt
       61 gccccccgac atgacgcatc cacatactgc ttgccgatcc ctgagaatgc agtgaatctg
      121 tatactgcgg ctcacccaga cggtcgtgta agcgatggct attttgccga catatcggcc
      181 gttgcgccat aggctccgcc atgttcggct aacgtcagct tttgcccacc atggatgttc
      241 agcgctttca gcccatagtt aacgtacaaa ccatcacctg gtgcggtcgt gaggcggaat
      301 ccataatcac cctcggcgac caccgttccg ttttgcgcaa tgtgtaacgt tttgtccgct
      361 tccacctcgt cgccctgtaa atcacgtaac gttaatgagc cacccgaccc aataaccgtt
      421 tgcgccttca ccagttgaac gccgacatgg ctatcatcgt gttccagcaa attgagcgtc
      481 gtcagagggt tattcgctat gggatcattc cacggtttag gcacgtcgat aagcacgtcg
      541 cccgtcccgt ttacctgata gttacggcct ttccaggtgt agtcacccgc gccgacaacc
      601 agcgtatcga cgctgatata tccctctgca agcgtcgcag caggaatatc cgtatcgaaa
      661 atgagcgtac cgccattcat ggccagtcca ccaatggatt gctctcccac gtttaccgat
      721 gtggtatttt cactgtcaga ctgcaacatc gcgtgagtaa gcgcagcggt gttgtcgcgt
      781 tccagagtga aggttctgtc tttcagttgg gcaacaccgg cgaattcagt ccctgttgca
      841 tgggtaaagc caaacatctt gtcgtaggat gacagctgca ctcgcatcaa gccatcgcct
      901 ttgagcgcat tggtcagcgt gtaatctcct gcactgttcg ttgatgcgag aatgtcgagc
      961 gtaccggggc ttgcaatggt gacttcactc gaacccatgc ccgttacccc atctgccgta
     1021 tgagacgcaa tggtcaggag gccgtcattg accagcgttt taccgctata agtattccct
     1081 gcattgaaca ccgtatggcc tgcatcatgg atcacttttc cgtctttatc gtcacctgta
     1141 atcagcatat cgacctggta gccggcatca ctgttattag tatgattaaa gacaaaaaca
     1201 ccttcgccag aaccaaactc tactttcgtc gcgttggtta tatacccggc atctgccgcc
     1261 gcctcaccgt gagctgcacc aatgtttagc gttcctacag caggttcaaa aacacctaag
     1321 taaacttctc caccttcgac attcagcgtg ccgctattcg tcagggttaa cgtacccgta
     1381 ccagatgtcc ctacgtacat attgaatgtt tcaagaagag agttctgccc atccaccctc
     1441 acgtcgccct tacttttgtc attgagagct atctgtgtat cacgcgcttt aactatacct
     1501 ccggtggtaa tattcagttc acccgtgcct aatacaccga cctgtagcaa ttgtgcgtta
     1561 gtagatgatg tctttaaatt ccagagactg tccgttgaaa tattaactat ccccttac
//
LOCUS       DAZSBF010000418         1614 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZSBF010000418 DAZSBF010000000
VERSION     DAZSBF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.425"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..240
                     /locus_tag="ACHXWD_004449"
     CDS             <1..240
                     /locus_tag="ACHXWD_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR7623108.1"
                     /translation="SDAFLPPESIFTLTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIK
                     NSQECPTNTDNVLNECFNNRCTLQICPYGLKQQSP"
     gene            complement(521..652)
                     /locus_tag="ACHXWD_004450"
     CDS             complement(521..652)
                     /locus_tag="ACHXWD_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR7623109.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1152..1228)
                     /locus_tag="ACHXWD_004451"
     tRNA            complement(1152..1228)
                     /locus_tag="ACHXWD_004451"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1192..1194),aa:Arg,seq:tct)
     gene            complement(1329..1404)
                     /locus_tag="ACHXWD_004452"
     tRNA            complement(1329..1404)
                     /locus_tag="ACHXWD_004452"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1369..1371),aa:Ile,seq:cat)
     gene            complement(1507..>1614)
                     /locus_tag="ACHXWD_004453"
     CDS             complement(1507..>1614)
                     /locus_tag="ACHXWD_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7623110.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          497 a          423 c          297 g          397 t
ORIGIN      
        1 tcggatgctt tcttacctcc tgaaagtata tttacactga ctcacacagg catcatgctc
       61 aataacaaag tgctacctgt aaccatttac aacgtagtac cattcaataa aacattctgg
      121 aattcaatca aaaatagcca ggaatgccct acaaatacag ataacgtatt gaatgaatgc
      181 tttaataacc gttgcactct gcaaatatgt ccttatggac taaaacaaca aagtccataa
      241 ggagtttact cacatctgac aaaatcaata taaacagccc ctccggagag gggctggaga
      301 gtggcgctat gtgccattgc atggtgccgg gtgcctcccg gtgaattcag taccagcacc
      361 tgaatccgcg attatcccat atacctactc gctgattgcc cctccgcaca gggggattca
      421 ccatgccagt ttcttttaac aaactccccg caaaccagac aacagtcaac cgcctgaatt
      481 gtgaagtatt taaaaatttc tcccgctaac tgatacccgg ctaacagtct ggcgttttct
      541 ttttcagcaa cgggaaagca acaaccacca cacccgccac cagcacaccg tcagccagca
      601 ctgacattat ccggctgctg caatgccatt cacaaaaaca gtaagcaatc actttttacc
      661 gtaacaggtg ataatccaga tatgtatcta ccccagatga gtaatccgaa gttcatccat
      721 accacaggtc ctggctattc tgttgtactc ctgaacaaga gcaaataatt ctgaattagc
      781 aaccatgaac tcatcgcaaa ccctctgtat agcatcacta ttcagaataa taacgtctct
      841 tcccgaaaga cgatcaggag tacagaacaa aactgtcaaa cggctgaagg cctttgctcg
      901 tgctgcattg actatatcaa tacgctgcct aaggatgaaa caccccgacg cctcatcaat
      961 attcactcta cccacaccat atgaatgata aatatttaat gctgaaaaaa ccattagacc
     1021 gtataacaaa cactcaatca atacttaaca gaacttttat ttttgacaaa cataaaatat
     1081 tttcaacaat atcctgagcc aggtatattt cagtataagg ctctgccgga aggaatctgg
     1141 aagaatgaat atggcgcgct gtactggatt cgaaccagtg accgattgct tagaaggcaa
     1201 ttgctctgtc cggctgagct aacaacgctg aataccgata atggaccgcc atcggggacc
     1261 cgcccccgca ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg
     1321 gtttgtgatg gtggcccttg ctggatttga accagcgact tggcgattat gagtcgctcg
     1381 ctctcaccac tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaattccgc
     1441 aataaaaaac ccgctcggcg gcgggttgta gaaactcttc taacgtcagg cataaaacgc
     1501 ccatagttat gacgaattta ccacagattc cggaaaaatc aacctagtta ccttttttaa
     1561 ctgccgctca gcccatgctt cttcaatatc aaaccgagtc accagcgcat cata
//
LOCUS       DAZSBF010000419         1605 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZSBF010000419 DAZSBF010000000
VERSION     DAZSBF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.426"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>1605
                     /locus_tag="ACHXWD_004454"
     CDS             <1..>1605
                     /locus_tag="ACHXWD_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFR7623111.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGS"
BASE COUNT          558 a          314 c          302 g          431 t
ORIGIN      
        1 tgtatataat attaacgtta cgtcaccttc cggacagcaa gaaacattta aaatactaat
       61 caatccagaa catctacggc agacacttga gcgtaaatct cttactgctg ttcagaaatc
      121 acaatgtgaa atcatcaccc ctaaaaaacc cggcgaagcg attcttcatg cttttaatgc
      181 cacctaccag caaatcaggg aaaatatgtc tgagtttgca cgttgccatt atgggtatat
      241 acaaatccct ccagtgacaa ctttccgcgc cgacggacca gaaactcccg aagaagaaaa
      301 gggttactgg tttcatgctt atcaacccga agatctttgt accatccata atccaatggg
      361 agatttgcag gattttattg cattggttaa agatgctaaa aaatttggta tcgatatcat
      421 tcctgattat acctttaact ttatgggaat tgggggtagt ggtaaaaatg acctggatta
      481 tccctctgct gatatacgag cgaagatcag taaagatata gaaggtggta tccctggcta
      541 ttggcaaggt caggttttga ttccattcat taaagatcca gtaacaaaag aacgtaaaca
      601 aatccatcca gaagatatac atctcactgc aaaagacttc gaagcaagta aagataacat
      661 ctctaaggat gaatgggaaa acctccatgc attaaaagaa aagcgtttaa atggaattcc
      721 taaaacaaca cccaaaagtg accaggttat tatgttgcaa aatcaatacg ttcgtgaaat
      781 gcgaaaatat ggcgtacgag gtttacgtta tgatgcggca aaacactcaa aacatgaaca
      841 aatagaaaga tcaataaccc caccgcttaa aaattataat gagcggttac acaatactaa
      901 cttatttaac ccaaaatatc ataaaaaagc cgttatgaat tacatggaat atctggtaac
      961 ttgtcagttg gatgaacaac aaatgtcatc actgctttat gaaagagatg atttaagcgc
     1021 cattgatttt tcattgctca tgaagacgat aaaagccttt tcattcgggg gagatctcct
     1081 aacccttgca tcaaaaccgg gttccacaat ctcaagcatc ccatcagaaa gacggatatt
     1141 gattaacatt aaccacgatt ttcctaacaa tggcaatctt ttcaatgact ttctatttaa
     1201 ccatcaacaa gatgaacaat tagcaatggc atatatggcc gctctcccgt tcagcagacc
     1261 tttagtttac tgggatggcc aggtattaaa atcaacgact gaaattaaaa attatgatgg
     1321 gtccacgcgt gtcggtggtg aggcgtggct taataaaggt tgctctacct atcagcagct
     1381 ctacaatgaa ttccacgcat tatatataga taaagcagga atatggagcg catttgaggg
     1441 tgtatttgca actaaaaacg ttctagcctt tagtcgtggg gattctgtga acattaatca
     1501 ctctcctcat gatggactag ttataataaa taaaggaaac gaagaagttg aaggtacctg
     1561 gcctaacaaa ttgcaacctg gaatatataa aaacatgggg agtaa
//
LOCUS       DAZSBF010000420         1600 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZSBF010000420 DAZSBF010000000
VERSION     DAZSBF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.427"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            27..584
                     /locus_tag="ACHXWD_004455"
     CDS             27..584
                     /locus_tag="ACHXWD_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623112.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            585..863
                     /locus_tag="ACHXWD_004456"
     CDS             585..863
                     /locus_tag="ACHXWD_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623113.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            866..1138
                     /locus_tag="ACHXWD_004457"
     CDS             866..1138
                     /locus_tag="ACHXWD_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR7623114.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            1138..1338
                     /locus_tag="ACHXWD_004458"
     CDS             1138..1338
                     /locus_tag="ACHXWD_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623115.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
BASE COUNT          548 a          296 c          324 g          432 t
ORIGIN      
        1 aacatcttat gagagtcaga acatgaatgc agtattcaga atttagagcg atgcttcagg
       61 catcaagaga acgcaacaag cacaactcct acgcttacac aaacaacccc accagctacg
      121 aagtcccaac ctttagcaaa tccgaacgta agaacattga agcggtcatt cgtagcatta
      181 cgccacgaga cagatttatg ccagtacgca aaacaacaaa gaatactatt aaaaattatc
      241 tggctaactt tgacagttac gagcagttac caagcaagct tgatgatgtt tttattgggt
      301 tctgtagaag tgaaggccac ccgaaataca acaaaaaact attttatctg ctaaagaatc
      361 ttgacgaaat taacgcatcc accgtgacaa atcatcttca gcgacaggca accagactta
      421 gttacgaatt accaagcgat aaatattgcg ctttgcttgc tgtgatgtgc gcgaaattaa
      481 ttggcattgt tgaacatcat attgccgttg ggaatattga gccaatggaa aacgagcaac
      541 cagatttcga gtttgacccc tatttagtta cagaggattt ttaaatgaat aaacacacat
      601 taaccagagt tacaaagaca gcctttgatg aactgaaccg aatcaaaaac attacaggtc
      661 tgccacactc agcagtgatg aagcttgcaa ttaaccgtga tattaacgaa caggatttgc
      721 ttaatagtcg cttagaggct gcaagacacc atatcaggat taagcaagat gaccttgata
      781 gtctgaaatc cttaaatgct tttggactgg atttagcaag gttactaagt atcaaaattc
      841 acaaactgtc tcaggaggtc tgattatgaa gcaatggcga gatgatatta aacggttctt
      901 tgcaacttac ttcatgatta attatgagac aggaatgctt gaatggattg atggtggaga
      961 ggttaagaca agagatgatg cttacggtaa tccaatgatt cgttatggca ctcgtgatta
     1021 ttatctgaag tatgtcatct ggtttttgca tcatgaagtt caggcaacta aaaagattat
     1081 cttcagggat ggtaataaaa gaaattgcca cccaaacaat ttactcctgg agatttaatg
     1141 ttatgtatta aattcgaata tttgacagat aaaatgatta agcatgtatc agacctgctc
     1201 atcaaagaag gtggtttcgg tgatgcttgc aatccaaaag atatcttcat tcatgccacc
     1261 agcccaaacg caacactaaa gacagcagtt acagcggaat ggtttgaacg aaataaagcg
     1321 gaattaggtt attggtagtc cgagacttta agagaaatta gaaaaacagc ttatcagacg
     1381 ctctcaatcg cattctaacg caaaaaacta tcttatttta gggtgggtaa taccactgta
     1441 caggctaccc ctgaaaaaga ctgtttagct cgttacagag cgattgagga ggtctggtaa
     1501 aaaggagatt ttaaaaatga acgaatatga attacagatt ttcattgata gcgacactgc
     1561 tatgatggtg caggctttcg ctgattccgg tgtatcaatt
//
LOCUS       DAZSBF010000421         1598 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZSBF010000421 DAZSBF010000000
VERSION     DAZSBF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.428"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            16..567
                     /locus_tag="ACHXWD_004459"
     CDS             16..567
                     /locus_tag="ACHXWD_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR7623116.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            627..>1598
                     /locus_tag="ACHXWD_004460"
     CDS             627..>1598
                     /locus_tag="ACHXWD_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7623117.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQ"
BASE COUNT          498 a          335 c          318 g          447 t
ORIGIN      
        1 atataaagga atattatgtc tgcttttaaa aagtcacttc ttgttgctgg cgtagcaatg
       61 attctttcca ataacgtatt tgctgacgaa ggtcacggca ttgttaaatt taaaggtgaa
      121 gttatctctg ctccatgttc aattaaacca ggtgacgaag acttaactgt aaatctgggt
      181 gaagtagcag ataccgttct gaaaagcgac cagaaatctt tggctgagcc tttcactatt
      241 cacctgcaag actgcatgtt gagccagggt ggaaccactt attcaaaagc gaaggttact
      301 ttcaccacag cgaatactat gacgggtcag actgacctgc tgaaaaacac caaagaaact
      361 gaaatcggtg gtgccacagg cgttggcgta cgtattctgg atagccagag cggcgaagtg
      421 actttaggta ctccggtagt aattacattc aacaatacta acagctatca ggaactgaac
      481 ttcaaagctc gtatggaatc cccgggcaaa gatgctaccc cgggcaacgt ttacgctcag
      541 gctgactata agattgctta cgagtaatca cattaggccg ggttcgcccg gccacatcat
      601 taacggatta atgataagtg gaacagatgt ataaaaaatt aaaattaacc acaataagcg
      661 aattgattaa aaatatttat tgttcattat ccgttctcat cattggttgt gcgtcagctt
      721 atgccgttga attcaacaaa gatttaatcg aagccgaaga tcgtgaaaac gttaaccttt
      781 cccaatttga aactgatggc caattacccg taggtaaata ctcattaaac gctcttatta
      841 ataataaaag gacgccaatc catctggacc tgcaatgggt attaattgat aaccaaactg
      901 cagtttgtct gactccagag caattaacat tattaggctt tactgatgag attattgaag
      961 aggctcagca aaacctgatc gatggttgtt accctatcga aaaagaaaaa caaattacaa
     1021 cttatctcga taaagggaaa atgcaattat ccatatctgc acctcaggca tggttaaaat
     1081 acaaagatgc aaactggacg cctcctgaac tttgggatca tggtattgct ggggcatttc
     1141 ttgactacaa tttatatgcc tctcattatg caccacatca gggcgataat tcacaaaata
     1201 taagttccta tgggcaggct ggggttaatc ttggggcctg gcgcctgcgt actgattacc
     1261 agtacgatca gtcatttaac aatggcaaaa gccaggcgaa caacctggat tttccgcgta
     1321 tttatttgtt tcgcccaatc ccagcaatta atgcaaaact aactataggt caatacgata
     1381 ctgaatcctc tattttcgac tctttccatt tttctggcgt ttcgttgaaa agcgatgaga
     1441 atatgttgcc gcctgatcta cgtggttacg caccgcaaat cacgggtgtt gcacaaacga
     1501 atgcaaaggt cactgtctca cagaacaacc gtattattta tcaagaaaat gttcctcctg
     1561 gcccatttgc tattactaat ttattcaata cattacag
//
LOCUS       DAZSBF010000422         1595 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZSBF010000422 DAZSBF010000000
VERSION     DAZSBF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.429"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHXWD_004461"
     CDS             <1..365
                     /locus_tag="ACHXWD_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7623118.1"
                     /translation="QEKLIWLRAQMKLTSLCWALKELAKDIWSRPWSEERRNDWQRWL
                     SLAANSDVPMMKNVAKTIGKRLYGILNAMRHGVSNGNAEALNSKIRLLRIKAKGYRNR
                     ERFKLGVMFHYGKLNMAF"
     gene            395..796
                     /locus_tag="ACHXWD_004462"
                     /pseudo
     CDS             395..796
                     /locus_tag="ACHXWD_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1381..>1595
                     /locus_tag="ACHXWD_004463"
     CDS             1381..>1595
                     /locus_tag="ACHXWD_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623119.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIG"
BASE COUNT          448 a          310 c          397 g          440 t
ORIGIN      
        1 ggcaggaaaa gctgatatgg ctgcgtgcgc agatgaagct gacgagcctg tgctgggcgc
       61 tgaaagaact ggcaaaggat atctggagca gaccatggag cgaggaaaga cgcaacgact
      121 ggcagagatg gctgtcgctg gcggccaaca gtgacgtccc catgatgaag aatgtggcta
      181 aaacgatagg gaaaaggttg tacggtatcc tgaacgccat gcgccatggc gtatcaaacg
      241 gaaatgcgga agcactgaac agcaagatca ggctgctgag aataaaagcc aagggctacc
      301 gcaaccggga gcgctttaag ctgggagtga tgttccacta tgggaaactg aatatggcgt
      361 tctaagcctt cccaccatga tcggggaaga cccagatatg aacaggcaca ttcagagtta
      421 ctttaaagag cttactgaac gtctgaggtg ttagagccat gactgccgca gcattgctgg
      481 cggaaatgcc tgaatcgagc agcctctcga gacgtgaaat cagcgcactt gtcggtgttg
      541 ctcaggtaaa ccgggactcg ggaactctgc gcggtcgacg aaccattttc ggtgattgtg
      601 ctggggaaga acagctctgt acatggcggc gcttgaggca ccccggttta atctggtgat
      661 aaaggcgttt tacatgcgcc tacttgcagc cggaaacgcc aaaaaagtgg cactggttgc
      721 ctgtatgcgt aagttgctga ccattatgaa cgcaatgctc agaaaaaacg aagaatggaa
      781 tgaatcgtat ttatgaaaac tggattgtgg gccataaata ttccaaaaag atagcccgct
      841 ccgtttatta ccggaacggg cgagagcatg acattgtctg taattataat gagactgctg
      901 attctactta tttttaattt caagaaaagc gttttctaca aagaattatg agatttaatg
      961 ttttgtttag acaatattat cattctgttc aataatgaaa gtcttcatca acaacaagta
     1021 aaaacaacgt ccctctcatc gaaccggact tgcagatttc ccgcatccgg ctctcttgca
     1081 acacatcaga ccttcacgtt cgggcggtta tggggaaggt ccagcaagct gtatgagccc
     1141 taactgtttg aataccagta gattaccaga aacttctatc gattgaagac attaccaact
     1201 agcgttacta aatgttgacc aagttttgtt cacttgtctg gttttgatag tagcattctg
     1261 cagagtattt tgatatgata aatatgcatt aacaatgata ttattgttaa tttcctagca
     1321 tgaaaattaa aagatcaact ttttatctcg aatatttttt acattatcag ttggtttctt
     1381 atgaatgaca attcattgct tagaaattct tcattattca tcgcctatat gggatgtgta
     1441 gggtgggtca gtgcttattc ttatggctgg ggaacctctt tttactatgg atttccatgg
     1501 tgggttgttg gtgcaggttt ggatgatgtt gccagaagtt tactctatgc tatcatagta
     1561 atgggtatac tttttaccgg ttgggggatt ggtat
//
LOCUS       DAZSBF010000423         1578 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZSBF010000423 DAZSBF010000000
VERSION     DAZSBF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.430"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(162..815)
                     /gene="ribB"
                     /locus_tag="ACHXWD_004464"
     CDS             complement(162..815)
                     /gene="ribB"
                     /locus_tag="ACHXWD_004464"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR7623120.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(919..1067)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1189..1479
                     /gene="ubiK"
                     /locus_tag="ACHXWD_004465"
     CDS             1189..1479
                     /gene="ubiK"
                     /locus_tag="ACHXWD_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR7623121.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          448 a          402 c          351 g          377 t
ORIGIN      
        1 ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga
       61 aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa gccagctaca
      121 acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca
      181 tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg
      241 gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac
      301 agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc ttcagtatga
      361 ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga
      421 ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca
      481 gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg
      541 ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct
      601 tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca
      661 acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca
      721 agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa
      781 ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat
      841 ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg
      901 gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa
      961 ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga
     1021 gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa
     1081 cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct
     1141 tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat gattgacccg
     1201 aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag
     1261 ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc
     1321 gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag
     1381 aaactggcgc ggcttgaaca aagaatgagc gaactggaaa atcgcagtac tgagattaaa
     1441 aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg
     1501 taaattatga attaatcttc taatttacag tgctttctat taccttatcg cttaattcca
     1561 ttcagaatat catcacaa
//
LOCUS       DAZSBF010000424         1571 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZSBF010000424 DAZSBF010000000
VERSION     DAZSBF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.431"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            388..1470
                     /locus_tag="ACHXWD_004466"
     CDS             388..1470
                     /locus_tag="ACHXWD_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR7623122.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          488 a          318 c          319 g          446 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatctcatt gattggatga atatttgtag tgcagtgaca tcatttttaa
      361 ataatagttc aaataaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta
      421 gctgcatcag tactgatggc gatgtctgct caggcagctg aaatttataa taaagacagt
      481 aacaagctgg atctgtacgg gaaagttaat gctaagcact acttctcctc taatgatgca
      541 gatgatggtg atactactta tgcccgtctt ggcttcaaag gtgaaaccca aatcaacgat
      601 caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgcgc tgaatctcaa
      661 ggttcctcca aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc
      721 tccatcgatt acggccgtaa ctacggtgta gcatacgaca tcggtgcgtg gactgacgtc
      781 ctgccagaat tcggtggtga cacttggact caaacagatg tgttcatgac tggtcgcacc
      841 actggtgttg caacttatcg taacaacgac ttctttggtc tggttgatgg tctgaacttt
      901 gctgctcagt atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa tggtgatggt
      961 ttcggtttct ccactactta tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa
     1021 tctgaccgca ctaataatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa
     1081 aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctacc
     1141 acctattctg aaacccagaa catgactgtt tttggtaata accatattgc caacaaagca
     1201 caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct
     1261 tacctgcaat ctaaaggaaa agacttgggt gcgtggggtg atcaggacct ggttgaatat
     1321 attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgattacaaa
     1381 atcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc
     1441 gttgctgtag gtatggttta ccagttctaa tttgattact aaaagatatg ttgcgggagg
     1501 ctttgcctcc ccaacatata agtggctccc tcaagccact tcctttagga gcacaacctt
     1561 gcttctaact a
//
LOCUS       DAZSBF010000425         1565 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZSBF010000425 DAZSBF010000000
VERSION     DAZSBF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.432"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..416)
                     /locus_tag="ACHXWD_004467"
     CDS             complement(<1..416)
                     /locus_tag="ACHXWD_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7623123.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKA"
     gene            1215..1511
                     /locus_tag="ACHXWD_004468"
     CDS             1215..1511
                     /locus_tag="ACHXWD_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR7623124.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          450 a          334 c          313 g          468 t
ORIGIN      
        1 tcagctttag cacgttcaat accagccttt ctacgctctg ctatcaagct tctttcaagc
       61 tgtgccaacg ttgacataat gcctaacatt gcttcaccca tagcgcttga taagtcaaac
      121 ccttctctaa ggctgataac tgtcacacct ttagcctgta gcgctttaac cgttgataac
      181 acatcaattg tgtcacgtcc aagacggtct acagctacca caactacggt gtcaccttct
      241 cgtgcataag ctaatagtct gctgaaaccg tttcttaaag aagctttgac agaaccactg
      301 acagcttcat cagtgaacca tttagcaatg ttgtaccctg cctcttcaat ttgcatttgc
      361 tggttagtga tggattggtc atctgtactc accctgcaat atccaagtat cgccataatg
      421 ttccccttag aagtctcaaa agttagtctc ataagtttag ctgtctcata atccaaaaac
      481 aaccttttga gactacttga gggtgcattc taccgttacg ttgaaagtgt ctcaaaagga
      541 agtcctttta agactggtta tgaaggagct atgaggttga ttaggggttg gtacaagccc
      601 aactaaccga atcaactatg aagattttag gagggtaact gtaaccatca ctttctaact
      661 ttcacgtagg aaattgttaa gtggtttgat agttacacca tgtgaccaag catctatata
      721 tcgttatata gccctgtata tagcactata tgtagtgtcg agagaggaac gactaacacg
      781 atacagttta ccctgaaaag agacttcaca gattctttat atatttcaat aagttagggt
      841 aaaaagggac tataccgaga ctgtatagaa tctgtgtaga attgcataga tacttggaaa
      901 tctactatta aattgcatag ggactcacct gatattgtcc tgtttttaac aggtctccta
      961 gaaaaccgaa tagacttcat tcaagggtca ttgaagtcat aatcatttca gtagaaaaac
     1021 caagtctcga ttttaaatgc aaatagcaat catattcaat aaggcaagac gatcaggatc
     1081 agtgaaaatg tcatttttgg cgtaattgac cttactttat gcatggtctt aacaaagaca
     1141 tcatctttcc gttgcagatc ttcgcccttt caaataacgt actttacaac tttcccgaac
     1201 aaggagttgt gcccgtgttg tatgttattt acgctcaaga caaagctgat agcctcgaaa
     1261 aacgcctttc cgttcgtccg gcacatttag cacgtttaca gttactgcat gatgaaggtc
     1321 gtttgttgac agctggtcca atgccagcgg tagacagtaa cgatccaggg gctgcgggat
     1381 ttactggctc aaccgttatt gctgaatttg aatcgctgga agcagctcag gcctgggccg
     1441 atgctgaccc gtacgtagcg gcaggtgtct atgaacacgt ttcagtgaaa ccatttaaga
     1501 aagtgttctg aatagagatt caatatttaa gggcacggtt tttgcaaccg tgttcttgtt
     1561 tggcc
//
LOCUS       DAZSBF010000426         1549 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZSBF010000426 DAZSBF010000000
VERSION     DAZSBF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.433"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            351..476
                     /locus_tag="ACHXWD_004469"
     CDS             351..476
                     /locus_tag="ACHXWD_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFR7623125.1"
                     /translation="MWLPVLFRFLVDSLAMLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="ACHXWD_004470"
     CDS             complement(921..1397)
                     /locus_tag="ACHXWD_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623126.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          359 a          405 c          384 g          401 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacacccca catcagtgcg taagtgctca atgtggcttc
      361 cggttctctt ccgcttcctc gtcgattcgc tcgctatgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag gaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctgtgcaac accttgttgc gtgttgtcgc atccaggatg
     1501 cgacaacgtt gtgtcatcct ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAZSBF010000427         1547 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZSBF010000427 DAZSBF010000000
VERSION     DAZSBF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.434"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            271..1386
                     /locus_tag="ACHXWD_004471"
                     /pseudo
     CDS             271..1386
                     /locus_tag="ACHXWD_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          607 a          279 c          220 g          441 t
ORIGIN      
        1 aaaaaaatca cttcacacac tatcagagaa cataataact ttttaaaatg agcgcatttt
       61 gatagttgtt aacgatttcc tttacgatac tatcactacc ctttttttat acacaaatat
      121 taccgggtgg agataaaagg gaaatcaaag gtaattatat caggaagata taacgcatta
      181 catttattgt gtgtagaaga aaatccattc ctgttctaat tatccaattt aaacatctta
      241 gcataaaaca aatgatgaat aaggaatttt atgtcaattg actttacccc aggtataata
      301 aatacatatc acggcgatat ttataactgc acaacaaata ccgataatgc taaaacgcca
      361 gacacaccga agtggccttg cgataattgg gaagaacaac aacccattaa ttccacattc
      421 tctggagaag gatatatctc tgatcaatat gatttagtgc aacaccaact tcaacaaatt
      481 aatgcgtgcc acacgaacac tacatataca aatgcagact actcaaaagt tgtggctcaa
      541 cttgtaagtc ttattactaa cattgaaacg ataagttcga cacaacttac gcaacagaca
      601 caatcaatac ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt
      661 cgaatcattg ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta
      721 gaagagggaa tacccataac attcagtgtc caaaccatgt tttcagatac taattttatt
      781 gctgaacaac gagctgactt accaactaat ataaaagata ttcaaagcct gtatcaaaaa
      841 atgacgaagt tatatattga gcacagtgaa aataaaaaca ggatgaaagt ctttgccgga
      901 gccaacttta ttgatttcaa tatgacagga cagaacctgt ccgggttcgt tctaaccttg
      961 tcaaggtttt atttcgaaga tctacttaat ataaatttca ctgacgcaaa tttaggtgac
     1021 actatttttt acataaagaa caccctaccc ccaaattata taaagatgga caatatcttg
     1081 acaaacaaat cgaaggttta ttttcaacat tattaaccat taatgacaac tttttacgag
     1141 caaaagcgga aattgcttcg acaataatta aatttttaga ggccaggata accaatcttt
     1201 catacaatga catattaaaa tatcagcaag aattccaaaa acaatgttat aagcaagtta
     1261 aagcctttac gacactctct cgatacaata aaattcaaac atgggcagaa atgtctgaat
     1321 atcagttcga agtatttcaa tacgagacgc ttaaccccaa aaaaatgtca cacacgcctt
     1381 atttaaagag gccacttcct aatgaaaaag atataaatta tggggtggaa atagaaatac
     1441 catctggcaa aagaattcgc ttatcaaatc attatcagaa tataatacca taatattgat
     1501 tattagcact ttataattat tgaatgaaaa tagattttat gtaattt
//
LOCUS       DAZSBF010000428         1536 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZSBF010000428 DAZSBF010000000
VERSION     DAZSBF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.435"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /locus_tag="ACHXWD_004472"
     CDS             complement(<1..1012)
                     /locus_tag="ACHXWD_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFR7623127.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGK"
     gene            1267..1513
                     /locus_tag="ACHXWD_004473"
                     /pseudo
     CDS             1267..1513
                     /locus_tag="ACHXWD_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          438 a          353 c          275 g          470 t
ORIGIN      
        1 tttttccccc aaaataaaca gacattggtg catcattata agctaatgct ttacccattt
       61 tacccgcagc taaatcaact gatgtggcag aaccattatc ttcaacaaat gcagttacac
      121 gaccaccaaa gtttgctaac tgaacatcct taccacccac ctgaactttt tcataacctg
      181 tagagttttg tttcaataat gtaaaaccgt tcgtgttggt attattatct tgaccaacaa
      241 aataagtctg cccttccacc tgtacttgat aaacttttgt tcctgcagta ccggtcgcat
      301 cagttgtatc ctttgtatct tgtacattac cggtaccaaa ataagtttca acatcagcat
      361 ttttataatt gctaccgaat gctttagcac ctcccacact accatcagtt gcatttacct
      421 tagttaagtc aggagcggct tcgctcttaa gtgcaaccgc agaggacatt ggaacttttc
      481 ctagtgctac gctaaagtta tcaagcccca aggttttagc atcaatttta tcaagggcaa
      541 tctcaatggt ttcaccatcg ttcgcaccta cttgaatttt catagtctga tcagaagcca
      601 gaacttttac accattaaat tgtgtctggt tagaaacacg atcaatttca ctaaggcgtt
      661 gtgtaatttc atcctgaatt gaattcaggt cggagtcaga gtttgtacca ttagtggcct
      721 gtactgacaa ttcacgaata cgctgtaagt tattgttgat ttcagaaagc gcaccttcag
      781 tggtctgcgc aacagaaata ccatcattcg cgttacggga agcctgagtc agacctttaa
      841 tatttgctgt aaaacggtta gcaatcgcct gacctgctgc gtcatcttta gcactgttaa
      901 tacgcaggcc agaagagaga cgttcaatgg cggagctcag agaagactga gatttgttca
      961 ggttattctg ggtcaacagc gacaggctgt ttgtattaat gacttgtgcc atgatctttt
     1021 ccttatcaat tacaacttga tgttattggg ctgttgccca cggtttctca ccgtacccct
     1081 tgtatcggca cctgaatttc gaactttaga aaattttagt ttctctcctg ttttttctta
     1141 acggctgaaa tatccgtaat atacaactct actctgcgca tcatcttcgt gaatcaacta
     1201 ttaaactttg cgcacaaatt gccgataacc cgcttatcta ctgttttcaa tcaaaaggaa
     1261 ttaggtatgg cttcatttac atctcttggc gttggttcga atctgccgct ggatacattg
     1321 ctaaacaacc tgaccatcgc tgaaaaaaaa cgcttaaacc caattactca gcaacagagt
     1381 gacaataccg cacgtctgac agcctatggc acattaaaaa tgtgcattag aaaaattcca
     1441 gactgcaaat actgcgttaa ataaagccga tctgttcaaa agcactaccg ttaccagtag
     1501 caatgaagac ctttgaggta gcctgagttt aacgga
//
LOCUS       DAZSBF010000429         1533 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZSBF010000429 DAZSBF010000000
VERSION     DAZSBF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.436"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(308..1471)
                     /locus_tag="ACHXWD_004474"
     CDS             complement(308..1471)
                     /locus_tag="ACHXWD_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR7623128.1"
                     /translation="MPNHDRFECQTAIDSDAILQLAWAVFKIFRIPVNQWTLATAALG
                     GVFLVSGLILLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFK
                     LDPVRYQARVDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKG
                     SQAAVNPFSERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQ
                     LTEAKYNLEQTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQN
                     SLLRLKPGDDAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVL
                     GTIELDPNDDIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          398 a          412 c          348 g          375 t
ORIGIN      
        1 ttttttaaat gtgactgtca tccttcagat aataaagcca tcatcgcaat agatggaaaa
       61 gcactcctgc tcttttttga ttagagccgt cgcaggagga acaactcata tcatcattgc
      121 gatctcgacg atgtatagtc tcatcctgcg gcagaacaag acggataaaa aatccaatga
      181 gagcacactc atccagaact tattaacata atgaatatta aagaaaaatc atcgcagcca
      241 atgaagtaat atgcaaaaat aattagccct tgcagggagc aaagttccat aatctccccc
      301 ttccccctca atgatccaaa tacaaataat gcatccaact ggtcattctt agcagcacct
      361 tccgcatcac cgaaacatgg ctgaaatggt cggagtaaac tgctacctgg gcgtaaatgc
      421 cgtcgggtaa ggcatcgata tcatcgttag ggtccagttc aatggttccc agcacgccgt
      481 ccgtgccggg aacgaccgtt aatgattgca ataccccctg cgcctgataa gaaccgcctg
      541 gcacgacagg taaaatacta gtcaatttgc cgtggaacac ctgcccaggt agcgcgttaa
      601 acaccacttc cgcatcatcg ccaggtttca gacgtaacag cgagttttgc cgaaattggg
      661 cgacaatttg ccgtttttgc tcggggatga agaccatcac cggacgcagc ggcaaggcag
      721 ctgcgtatgt acctgggcgg atcagtacct gagtaacgta gccattgctc ggcgcgcgaa
      781 tgacagtctg ctcaaggtta tattttgctt cagtaagttg cgctcttaag ctcacaatct
      841 gcgattgctc gccgttaacc atactgtcga gctggctctg gatctgcgcc tgctccgcca
      901 ccgagccttt caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac
      961 gttccgagaa cggattcacc gccgcctggc tgcctttcag gtaacgttga taatttttaa
     1021 acagacggtc gcgctccgct gaaacctggg tggtgttggc ctgcgcttca gtgagctgcg
     1081 cacgcagcgt ctttatatta tgcgtcgccg tcatcaggtc agcctgaagt ctgtcaactc
     1141 gcgcctggta acgaaccggg tcgagcttaa aaagcacctc gcccttttga ataagctgat
     1201 tattcttgtc agtgacttca gtaacaattc ccgtcacctg tggcgtgata gggatcgcta
     1261 tcactgcctt ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac aaaatcaaac
     1321 cactcaccag aaacacgcct cccagcgccg ccgtcgccag cgtccactga ttcaccggaa
     1381 tacgaaagat tttaaagacc gcccacgcca gctgcaaaat ggcatctgaa tcgatcgcag
     1441 tttggcattc gaatcgatca tgatttggca tccatctccg atcacagttt ggcatcacaa
     1501 tcgatcacga tttggcatac ttccgatcat tga
//
LOCUS       DAZSBF010000430         1516 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZSBF010000430 DAZSBF010000000
VERSION     DAZSBF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.437"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1516)
                     /locus_tag="ACHXWD_004475"
     CDS             complement(<1..>1516)
                     /locus_tag="ACHXWD_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623129.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     PVDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQ
                     WQYDDHGWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAY
                     SEQGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAY
                     ELATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVH
                     TTAANLDIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAE
                     KTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          510 c          442 g          342 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc tgtccgggtg cagttccggg tccggcagcc gatttcctgc cgggtctgtt
      301 gcatacggga tatcgatatc caggttcgct gccgtggtgt ggacgcccgt cagcctcccc
      361 gtgtcgctgt aacggtactc ccggctctcc tgcgggccac tgatgcggat tagctgtccg
      421 ttgtcgttcc agtcgtagtc acggtcaagc tgcggaaggt tcaggtgcct gctccggagc
      481 tggccgctgg tattccaggc ggtggcaagt tcatatgcct ccccgccgaa gctgcgggcc
      541 gtctcacggt gcagccggtc ccgcgtgtac tcgaccagcg gtgttccgcc cagcttcatc
      601 cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcaggcc gtccggctcc
      661 tgacgggtcg ccagcccctg ttcgctgtac gcgtgccccg tctcatgctc ccacagcatc
      721 tcccccgtct ccggggtctc caccgtctgc cgttcgcccg tcagacggcc tttatcgtca
      781 tagccgtagt ggaccgccac acggtggcct tcactcaggt ggctgatttc tgtcagccag
      841 ccgtggtcgt catactgcca ctgctccgcc ggttcaccgt tcaccgtgcg ccgggtgata
      901 cggtccgacg catcgtaatg ccagagggtg ataagcccct catcttcact ctgcgtgagt
      961 tttccggtca ggtcatactg gtaacgttgc gtccggccgt caaagccgcg ctgctccgtc
     1021 agcctgtcga ccgggtcata ccggaacgtg gactggctgc cgttctcgtt ggtcagcaca
     1081 gtgatgcgcc cggcggcgtc ataccccatg cttcgcgtca gaccgccctg cgtggtgctg
     1141 acggcctttc cccacgcatc atactgtatc tcactgcggc tgccgtccgg ggcaacgata
     1201 gcggtgaggt ctcctgcggc gctgtactca taacgggttt cacgcccctg cgcatccctc
     1261 tgactgacca gttgaccgcg cgggttataa ctgctgtaag tgctgatgcc ttcttcccgg
     1321 tgaacggcga tttgttgacc gtaccggtca tactcatacc gcgtcgtgta ccccgagcag
     1381 tccgtaaacg cctgaagctg accgtaccgg tttcgcgtta ttctccgcct caccccggtt
     1441 ccgtcttcca ccgcacaggg caggccactg cgggaagaat cataaaacca gcgcgtaatg
     1501 ttaccgttgc gcgagg
//
LOCUS       DAZSBF010000431         1516 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZSBF010000431 DAZSBF010000000
VERSION     DAZSBF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.438"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..63)
                     /locus_tag="ACHXWD_004476"
     CDS             complement(<1..63)
                     /locus_tag="ACHXWD_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623130.1"
                     /translation="MGIKVQRLSLWMINTVLLLLP"
     gene            complement(116..520)
                     /locus_tag="ACHXWD_004477"
     CDS             complement(116..520)
                     /locus_tag="ACHXWD_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR7623131.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(741..1472)
                     /gene="bluR"
                     /locus_tag="ACHXWD_004478"
     CDS             complement(741..1472)
                     /gene="bluR"
                     /locus_tag="ACHXWD_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR7623132.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          399 a          336 c          327 g          454 t
ORIGIN      
        1 gggtagcaat aaaaggacag tatttatcat ccatagggat agtctctgta cttttattcc
       61 cattatgcta atgccttact gaattatgaa gcatttctta agcatccaac tttagctaga
      121 ttaatggttt attattttct acatcttcaa tatataaaag cgtattatca atggcgtagt
      181 aactgcgttt gttatgatta acatcagtaa cccaacggaa aacgcccgcg cctgccagtg
      241 ttgaacagta ttcccgaaat gtagattttc cgtaaatatg aagcaatgcg gcctctttta
      301 ttttagcagg gttcttggtc gtactaactt ttaacaggtt cctggttcct cttaataaca
      361 aaaccgtgtc atcgtgagta ataattctga tgttatccgt agccagataa taaatgtaat
      421 gtgcaatacg gtgatgtttt aattctgaat aaaaccagga gaagttttgc tcttttctca
      481 cttgctcaaa catcttttga aaaacaacga cctgatccat caggataata acctcttgtt
      541 agttgtgaga ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg tctgtccttt
      601 ggctgtgata tgtacagagt gtgatagagg gattacctga attcttccgg tgagcatttt
      661 gcaacggacc agctccggta caaatgctgt tgtgggttca gattataaca ttctgtctaa
      721 ggggcgggat aaaggtgaaa ttagggggca tgaaagatga ctttataacc ttgctcaccc
      781 caatgttgta aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca gacaatcaat
      841 gtttgcgtcg ggaacagttc cgggtgcggc gattcaatgg gatcagcaag aacagaaatg
      901 tgccatcctg acaatgataa acgccatgct tccagccaca atcgtgctct ctcttcaaca
      961 ttccacgcca tcagaatggc ttctttaccg ggcttacggc gcatttcgaa aagtgaggct
     1021 gctgcatatt caattaatgc gccgtcaaac atactgctca taatgcggga ggtgttgtga
     1081 tcaagcacga gacgctggcg aacaggaagg taaacatgat taatcaattg atctactggg
     1141 tactctcgac ccagtgaaat aattctcgcg cgtagtttgg caggattagc catacgaaga
     1201 attgacatca tctcttcttg caggcggttc cagtcatctt ccgtatcctg gctggtggtt
     1261 tccagtaatg ctttaacttt gcctacaggg acgccattac ttatccaacg cttgatctct
     1321 tcgatgcgtt gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact gcgctgtggt
     1381 tttaacaaac cgtagcggcg ttgccaggcc cggagagtga caggattaat cccgcaacgt
     1441 tcagcaacat caccaatgct gtaataagcc acaattcctc cttgcggtca taaatctccg
     1501 ccgcctgtac acgacc
//
LOCUS       DAZSBF010000432         1507 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZSBF010000432 DAZSBF010000000
VERSION     DAZSBF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.439"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..937)
                     /locus_tag="ACHXWD_004479"
     CDS             complement(<1..937)
                     /locus_tag="ACHXWD_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR7623133.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQ"
     gene            complement(963..>1507)
                     /gene="rfaY"
                     /locus_tag="ACHXWD_004480"
     CDS             complement(963..>1507)
                     /gene="rfaY"
                     /locus_tag="ACHXWD_004480"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR7623134.1"
                     /translation="DTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRS
                     AGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLH
                     ALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGY
                     YSVIYRTKLRKFIKKLKGKA"
BASE COUNT          468 a          319 c          262 g          458 t
ORIGIN      
        1 gttgatattt cacttgatac tgcttttcat tcgtggctgg aataaacgcc acatcattcc
       61 agcaagaagc ctgataagcc tcattaaaat acttctggca gggataattc gtccagtcgt
      121 tccagggctt cgttacgcca acataatgaa tcagcttagt atcatcggta atggtctttt
      181 tataatcttc atcgctttta ttttttaact cataatcgat gccataaaag caatcataat
      241 cgccgctaat aaaaatacaa tgaccaaaga acagaatatt taaaatatcc tgatcaaggc
      301 atttatactt cgtgtgctgc gggtcacttg ccagcatagc aatggcttta ttcataaaat
      361 catttttcgc ccatgcttca agctgcagat atactacacc ggcgttgaaa taattccctg
      421 gaagtccctc tatagccaga cgcttcgctg gtttttcctg cataaaaggc acatctttaa
      481 taacagctgc aaacttatcc ccatcaaaat taatatcaag caatccagct aatgagcctt
      541 tacagatgac atcagcatcg atatataaca gattttcaat agtttcactc agatactgga
      601 acgcaactaa acggaagaat gttgcataag accactgttt caggctgggg aagatatcaa
      661 taaaggcact ggagacttta taaattctta cttcaacgtt agcatttttg ctggtaaaag
      721 cattaacacg ttgtagataa tcttcatcaa tgtagtcggt aaagagatga aacttaaccg
      781 caaggtccgg attatgcata acaactgatt gcatagatac tgccgcacca aataaaaagt
      841 tacggtcaat accgtaagcg atatttaaca cacgatcggt gttagcaatt tccccagaga
      901 cagcgacttc agtaatactc tcagctaata aatccatgtt ggttccgttt tgactgtgtg
      961 gtttacgctt tgccttttaa ttttttaata aatttgcgta gtttggttct gtagattact
     1021 gaatagtagc cataatcttt tatttcgttg gcaataccca ggtgacgttc catggccaga
     1081 cgatcacgag ctttacgctc tgccgtacag cttttaccgg ataggtcaat tatcctgaca
     1141 ccatctttac taacgataaa gttaccccgg tgtgggtcgc ccgatagcat atttaaggca
     1201 tgcaattttt ccattgaagc cttaatttct gctttaacat cttctggaat gatcggcata
     1261 tcattgagct ctacgccctc gacatactcg ataagcatga taaaaacact ggcatagttg
     1321 aaaattttac gctcagccag aaaataaaaa tcattaggaa aagtaagacc tgcacttctg
     1381 acacgatcgg tttcaacgat caaattctga tagtagtcac cttttacaaa agattttaaa
     1441 aaacgttcat tccttttcgt tttcggggca aagactttaa aaacatagcg acaatatttc
     1501 gtatcaa
//
LOCUS       DAZSBF010000433         1503 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZSBF010000433 DAZSBF010000000
VERSION     DAZSBF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.440"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..312
                     /locus_tag="ACHXWD_004481"
     CDS             <1..312
                     /locus_tag="ACHXWD_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFR7623135.1"
                     /translation="LCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRA
                     DESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIE
                     S"
     gene            349..>1503
                     /locus_tag="ACHXWD_004482"
     CDS             349..>1503
                     /locus_tag="ACHXWD_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR7623136.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSG"
BASE COUNT          478 a          280 c          294 g          451 t
ORIGIN      
        1 ttatgtggtg acacttatat ttccagaggt cccgttttat atgcaattga gcttgagagc
       61 gatattttaa tcaaaaaaaa cctgctgaac gggaaatatt ttgactcagg atacataccc
      121 tgtagtagag ctgatgaatc tatccagttc tcttatgctg atatggaatc tttttcatat
      181 tcaacctcac cagagggcaa acaatacatt gaaggctgtt tttatttaaa caagactaaa
      241 atagtcagaa aacttatacc atttggtcaa accattcttc gcaaagtaac tttctccaat
      301 atagagagtt aatattttcg agtgttataa ttaattaaag ggatttctat gtaccttact
      361 gataatatta tcaaactgtc gaataaaata ggtcagcgtg tttatactgt tgtctctgaa
      421 atgtcgattg aagcatggat aacaaaagaa ccgcagcctt ttgtttatcg ccgtagcggc
      481 gcttaccaga aactcaccat tggctcagac tggggtcaac tgtttgactg tgcctggatg
      541 cgagtttatg gtaaacgtcc agcagataaa tttagacaca aactcgtcgc tctcgttgat
      601 atcggtggcg aaggtcttat tgtggataag aatggttcac ccatctgcgg catcacgaat
      661 aaagcatcat cttatggcgt accgccagat aaaccaggta aatgggtggt tgacctttct
      721 ctcgttagtg aaaatgacga ggttgagttt tggattgatg ctgcctgcaa cgatttattt
      781 ggctatgtta ctaatggagg gattattact gatgttcata tcgcgacacg taatcaacta
      841 ctgaaatccc tttattatga tattgaggtg cttttcgact ggattaatga tggtcagaag
      901 tttgaatcca tacatcctaa aggtatcacc ccagaacaat ttattgccaa aagaagtgag
      961 cgtactgacg aaatcataag tactctcgaa tatattgata atacgttaat tacctttagc
     1021 aatgaagaaa taattaaatg tcaaattgca atccaatcca taatcaataa aaacaataat
     1081 ccttcagagt tcaggattat ggctactggt catgcccatt tagatatagc gtggatgtgg
     1141 cctttaagag aaggacgcag aaaagcaata agaacatttg ccaccgccct tgcaaacatt
     1201 gaaaaatatc ctgattacat ttttggcgct agtcagtacc aactttttca ctggataaaa
     1261 aaagattatc cctatttctt cgaaaagtta aaaaaacaaa tcgccgcagg ccgctttgag
     1321 ttacaaggtt gcttctgggt cgaatgcgat ttaaatctgg taagtggtga atcgctgata
     1381 aggcaaatca tgcatgggac acgattcaca ctgcagaatt tttcaaaaaa gataaattat
     1441 gtctggcaac cggatgtctt tggattcccc gcaacgcttc cacaaattct caaaaaatca
     1501 ggc
//
LOCUS       DAZSBF010000434         1491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZSBF010000434 DAZSBF010000000
VERSION     DAZSBF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.441"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..225
                     /locus_tag="ACHXWD_004483"
     CDS             <1..225
                     /locus_tag="ACHXWD_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR7623137.1"
                     /translation="IQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            337..600
                     /locus_tag="ACHXWD_004484"
                     /pseudo
     CDS             337..600
                     /locus_tag="ACHXWD_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(637..1194)
                     /locus_tag="ACHXWD_004485"
     CDS             complement(637..1194)
                     /locus_tag="ACHXWD_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR7623138.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1191..1466)
                     /locus_tag="ACHXWD_004486"
     CDS             complement(1191..1466)
                     /locus_tag="ACHXWD_004486"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR7623139.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
BASE COUNT          396 a          320 c          382 g          393 t
ORIGIN      
        1 atacagcagg agccgaatgg ctggaagacc gtcaccacac agttctacat ccaggaattt
       61 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
      121 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      181 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      241 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      301 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      361 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      421 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      481 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      541 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      601 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      661 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      721 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      781 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      841 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      901 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      961 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
     1021 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1081 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1141 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1201 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1261 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1321 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1381 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1441 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca g
//
LOCUS       DAZSBF010000435         1461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZSBF010000435 DAZSBF010000000
VERSION     DAZSBF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.442"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="ACHXWD_004487"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="ACHXWD_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR7623140.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="ACHXWD_004488"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="ACHXWD_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR7623141.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          446 a          276 c          337 g          402 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt g
//
LOCUS       DAZSBF010000436         1459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZSBF010000436 DAZSBF010000000
VERSION     DAZSBF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.443"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHXWD_004489"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHXWD_004489"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR7623142.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAAIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..>1459)
                     /gene="cadB"
                     /locus_tag="ACHXWD_004490"
     CDS             complement(1092..>1459)
                     /gene="cadB"
                     /locus_tag="ACHXWD_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR7623143.1"
                     /translation="LLAAVKMTALIILITLMNSAGGKASDLFGELTGIAVLLTMLPYF
                     YSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYAR
                     KMHERQSHSMDNHTASNAH"
BASE COUNT          406 a          320 c          365 g          368 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggcagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggattatcag ggcagtcatt
     1441 ttcactgcag ccagcagca
//
LOCUS       DAZSBF010000437         1431 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZSBF010000437 DAZSBF010000000
VERSION     DAZSBF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.444"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHXWD_004491"
     CDS             <1..439
                     /gene="lpfB-O113"
                     /locus_tag="ACHXWD_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR7623144.1"
                     /translation="SQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLE
                     IAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDV
                     PLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHXWD_004492"
     CDS             464..>1431
                     /gene="lpfC-O113"
                     /locus_tag="ACHXWD_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR7623145.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          373 t
ORIGIN      
        1 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
       61 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      121 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      181 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      241 gatagcagag aatcccaacc caattcatat cagtgtgatg gatgtcatcg ttgatggcca
      301 tgatgtgccc ctcaatatga tccgtccttt tgaaacgctc acgttacctt tacctgctaa
      361 ttctgcggga ggccaaatga cctggcgatt tattaatgat tacggcgcag tcagtgaccc
      421 gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga cgaccagaat
      481 agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga tggccgaggt
      541 cagtgtattc aatccggcgc ttctggaaat cgaccatcaa tccggagtcg atattcgcca
      601 gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt ttatcaacgg
      661 taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg ctgatctgca
      721 cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag ttgatgcgct
      781 caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac gtattgaagg
      841 ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc cccaaatcta
      901 catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg ggattaacgc
      961 gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt atggttcaca
     1021 agagacagat accagttatc tcaatctgcg caatggactg aatattggac cgtggcggct
     1081 acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact ccattagtac
     1141 ctggatacag cgcgatattg ccgcgttaag aagccagatt atgattggtg atacgtggac
     1201 ggcgagcgat attttcgaca gtacgcaaat tcgcggcgcg cgtttgtata ctgataacga
     1261 tatgctaccc gccagccaga atggctttgc tcctgtggtt cgtgggattg caaagtccaa
     1321 cgccaccgtc atcattcggc agaatggcta cgtgatttat cagtctgccg ttccacaagg
     1381 tgcttttgag atcaccgatc tcaacaccgc cagtacaggt ggcgatttgg a
//
LOCUS       DAZSBF010000438         1426 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZSBF010000438 DAZSBF010000000
VERSION     DAZSBF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.445"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHXWD_004493"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHXWD_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR7623146.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHXWD_004494"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHXWD_004494"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR7623147.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHXWD_004495"
     CDS             complement(822..>1426)
                     /locus_tag="ACHXWD_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR7623148.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZSBF010000439         1420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZSBF010000439 DAZSBF010000000
VERSION     DAZSBF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.446"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHXWD_004496"
     CDS             <1..>1420
                     /gene="rhsC"
                     /locus_tag="ACHXWD_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HFR7623149.1"
                     /translation="VLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRL
                     SAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAH
                     RHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGL
                     KRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASA
                     FYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATED
                     ATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQL
                     IAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDA
                     AGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHW
                     HYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          421 c          436 g          225 t
ORIGIN      
        1 tggtactgac cacgcaggcg cagcgggcag aagaagcccg gcagcaggcc atttccggcg
       61 ggacggaacc gtccgctttt cctgataccc tgccgggtta caccgaatat ggccgggaca
      121 acggcatccg tctgtctgcc gtgtggctga cgcacgaccc ggaatacccg gagaatttac
      181 ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg cgaactggcg gcagtgtatg
      241 accgcagcaa tacacaggtg cgcagcttta cttacgatga taaataccgg ggccggatgg
      301 tggcgcaccg tcacacgggc cggccggaaa tccgttaccg ttacgacagc gacgggcggg
      361 tgacagaaca gctaaacccg gcaggcttaa gctacacgta tcagtatgag aaggaccgca
      421 tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggt gaagccgggc
      481 tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt cagtttgatg
      541 cggtgggcag gctcagggca cagacggatg ccgcaggccg gacgactgag tacagcccgg
      601 atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca tcggcgtttt
      661 actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg gaaatgcgcc
      721 gcaaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc gatatcatcc
      781 gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat gccaccggca
      841 gccggaaaac catgacgtgg agccgttacg gtcagttgct gagcttcacc gactgttccg
      901 gttatgtaac ccgttatgac catgaccgct tcgggcagat gacggcggtg caccgcgagg
      961 aagggctgag tcagtaccgc gcatacgaca gccgtggaca gttaattgcc gtgaaagaca
     1021 cgcagggcca tgaaacgcgg tatgaataca acgccgccgg tgacctgacc accgtcattg
     1081 ccccggacgg cagcagaaac gggacacagt acgatgcgtg gggaaaagct atctgtacca
     1141 cgcagggcgg tctgacgcgc agtatggaat acgatgctgc cggacgggtc atccgcctga
     1201 ccagtgaaaa cggcagccac accaccttcc gttacgatgt actcgacctg ctgatacagg
     1261 aaaccggctt tgacggccgc acacagcgtt atcaccacga cctgaccggc aaacttatcc
     1321 gcagcgagga tgaggggctg gtcacccact ggcactatga cgaagcagac cgcctcacgc
     1381 accgcaccgt gaatggcgaa accgcagagc ggtggcagta
//
LOCUS       DAZSBF010000440         1419 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZSBF010000440 DAZSBF010000000
VERSION     DAZSBF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.447"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..151
                     /locus_tag="ACHXWD_004497"
     CDS             <1..151
                     /locus_tag="ACHXWD_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFR7623150.1"
                     /translation="NNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSG
                     KRFER"
     gene            complement(309..431)
                     /locus_tag="ACHXWD_004498"
     CDS             complement(309..431)
                     /locus_tag="ACHXWD_004498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623151.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(504..1244)
                     /gene="phoN2"
                     /locus_tag="ACHXWD_004499"
     CDS             complement(504..1244)
                     /gene="phoN2"
                     /locus_tag="ACHXWD_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFR7623152.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
BASE COUNT          426 a          274 c          300 g          419 t
ORIGIN      
        1 caataatgga ttggttgatg taaacaaaaa atttcaaaaa gcaaatagtg gggacactat
       61 gcttgacaat gcaatgaaaa gcaaagattc aaaaatgatt gattttttat taaaaaatgg
      121 agcggtatca ggcaaacgat ttgagaggtg acctaaaata tatttaggtt gtgccctgaa
      181 tctgaagttg tcatttctgt tatattgatc gacatagggg atggtgcaga ccgttcccct
      241 gcaacaccac attgcatgtg gtgaacggaa ccgccccgag tttcctagag aatgttttat
      301 ctgtgaactc aggttgccag atcatcgttt ccgatggcag cataataagc tttttctgct
      361 tctgccggag ggatatgtcc cagcctttcc agtaatccct taaattcagg ggtaaaacgt
      421 ggtgtgccca tagactcctc ctatgctcat atagggtaga tttgtctatg ggccctccat
      481 agcctgatac aggctgtcca gctttatggg gtcagttcat tggtaggagt atttaattct
      541 tcaaactctt ttttggcttc gctaaggctt ttggtaaatt caggtgtatt atgaagtact
      601 gcaacaaccg atgctcccat taaacgccca gcctctacat cgctttgcca atgcgcaccg
      661 cagatgaccc gactttctcc aaactcatat ccacgtcgaa gtatttccgc tttacgttga
      721 ggattaatct ccgcaagtat cagtgctact gcccaaccaa aggatgcatg accagaggga
      781 taagagccag tgatagccat gttctcatct ttatcaggtg tacaggttgc gtctttataa
      841 ataacgaatg gacgaactct tttataatat tctttggcgt tacgcacggc gtaatcatgg
      901 ctatcctgta gtacttgtgt taacaactga tataagattg gggtattttc ccttgaaata
      961 tccatgccat aagcatctga aaatgcaaga ccaaaattct cgtagtatgc atcttcgcta
     1021 gcgagacgta cacgattagc atcccggagc gagcggccga attcataatg agctttgtca
     1081 gcctgaaata ctactgaatc ctctgccgga ggcggcggaa gtattgacaa actgtctggt
     1141 gaagtttgtt gagtgagaaa accttctgcc ttcagagcat ttgctgaggg gataaaaatc
     1201 atatttgtag caatacaaaa aagaagaaag tttttggttt tcataattta tttccttagc
     1261 ataattaatt gatgattttg gtcagctgta aaaaacgagc tgtgttacaa aaaacacaag
     1321 tcatgcggaa aaatttgaac agagtgatgg aaaaacaaaa aatagtctta tattaaagta
     1381 ataacattat ttttcatatt cacgaaaaat aatgtggta
//
LOCUS       DAZSBF010000441         1412 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZSBF010000441 DAZSBF010000000
VERSION     DAZSBF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.448"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1412)
                     /locus_tag="ACHXWD_004500"
     CDS             complement(<1..>1412)
                     /locus_tag="ACHXWD_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR7623153.1"
                     /translation="IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEM
                     NFMETNGKVNYQLLSTPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNM
                     KKNGDFVRTLSACTLNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHF
                     ELSQLSAKDFFDDFYWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAI
                     TKNLTEVLFILMEKYKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKI
                     LNMCNLSQETILTLLAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYL
                     LAAKDSHDLPGLFFALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNG
                     HTKAIMAYGDILKILPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKN
                     YSFTTRRLVEMLSATNKDGI"
BASE COUNT          423 a          268 c          219 g          502 t
ORIGIN      
        1 gaataccatc tttattagtt gcggacagca tttcgaccaa tcgtcttgtg gtaaaactat
       61 agttttttag aatattacaa aaggcacgta ttgtttcggt atggccatta taaaatgcca
      121 taaaaagtcc tgatgtaccg ttgggatttt ttatccataa taattcatct atatattcct
      181 ggtgaggggg gagtattttt agtatatctc catacgccat aatggccttt gtgtgcccat
      241 tttgaagtgc cataaacaac ccatgcttta ctttcaataa ttctttaatt tgttcactac
      301 taagcatttt cttattaagc agacttccga acatacgaat actatctgca tgtccattct
      361 gcaatgcaaa aaataaccca ggtaaatcat ggctgtcttt cgcagctaat agatataatc
      421 tcttatcgga agttaacggg gttttaaaga ctatttcccc ataggctttt attgtgtcta
      481 catgtccgtt cataaaagac atgcataaac ccggcacatt gtttgcccct accgcagcaa
      541 gcaatgtaag tattgtttct tgactaagat tgcacatatt taatatctta ccatatactt
      601 gaataatgtc tgaacatccg ttttgaagtg ctatataaaa tgcaggagta ccatcagagc
      661 ttctcgtggc cagaaggttc accagtttag attgagaaat ttcaccattt ttgtattttt
      721 ccatcagtat aaataatact tctgtcagat tctttgttat cgcatgatat ataactctgg
      781 aattgatcaa ttcatctggt aaaagtttca ctgtgtttgt tagaggggaa ttatgcctat
      841 cttcaaatat tacctgcccg ggttgctcca gaccataaat tttccaataa aaatcatcaa
      901 aaaaatcctt agcggataat tgactcagtt cgaaatggct atcacaagta aatacagtac
      961 gtttatgagt aacagttcga ttaggatcga aaaactgaac aacccatttt tcactttctt
     1021 gtacgcgctt tattttcaac cctaacgcca tttggtggtt taatgtgcat gctgataatg
     1081 ttctgacaaa gtctccattt tttttcatgt tataaaaaaa attagtaata actgttcccc
     1141 actttttatt ttccacaaaa taaatataag ctggtgattt tgaaaaatct tccgtttttt
     1201 ccaaacatac attttttgca atagcatcag gggtgctaag aagctgatag tttaccttcc
     1261 catttgtttc cataaaattc atttcccaat aggaagctag gtgacggcaa actatttgtc
     1321 ttccatatat atcttttgcc actccattta tatttacctt cacatttctc ttttctgaaa
     1381 aataaggtgc tgttttattc ttaaactgaa tt
//
LOCUS       DAZSBF010000442         1392 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZSBF010000442 DAZSBF010000000
VERSION     DAZSBF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.449"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHXWD_004501"
     CDS             <1..459
                     /locus_tag="ACHXWD_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7623154.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHXWD_004502"
     CDS             477..1115
                     /locus_tag="ACHXWD_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR7623155.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZSBF010000443         1384 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZSBF010000443 DAZSBF010000000
VERSION     DAZSBF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.450"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..404)
                     /gene="mxiG"
                     /locus_tag="ACHXWD_004503"
     CDS             complement(<1..404)
                     /gene="mxiG"
                     /locus_tag="ACHXWD_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HFR7623156.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIV"
     gene            complement(413..871)
                     /gene="ipgF"
                     /locus_tag="ACHXWD_004504"
     CDS             complement(413..871)
                     /gene="ipgF"
                     /locus_tag="ACHXWD_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFR7623157.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(871..1233)
                     /gene="ipgE"
                     /locus_tag="ACHXWD_004505"
     CDS             complement(871..1233)
                     /gene="ipgE"
                     /locus_tag="ACHXWD_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFR7623158.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(1243..>1384)
                     /locus_tag="ACHXWD_004506"
     CDS             complement(1243..>1384)
                     /locus_tag="ACHXWD_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol phosphate phosphatase SopB"
                     /protein_id="HFR7623159.1"
                     /translation="IQEMNTGVPGNKVMKKLPLSSLELSYSERIGDSKIWNMVKGYSS
                     FV"
BASE COUNT          426 a          308 c          177 g          473 t
ORIGIN      
        1 acaataacag caaagaaaaa aaacaccgag tggttcttat acattccgtt tagtatatgg
       61 cctctcgatt tatcctccct catattttta aggtgaaaag aaatcccgtc atattcaaat
      121 gaattaaatt gtgaattgat atcaatttgc tcaccttttt catcactaaa ttgacagata
      181 ttatctttgc ttatggtcaa ataaattata ccatccgatt tactatctgt tacaggaata
      241 atattttctg ggaaattgtc gtttccaaac tcaagtgttt ctatcgtccg tccgagaaca
      301 attaagttat tcccataata taacggaaac tcatccccga caccgtttgt gagtttcact
      361 aataacctga atggtgcaag atttgagttc tttgcctcag acataaatcc tcttatatcc
      421 ttcgattatt ctgcttgctc tctgctgcta tccgcaaata gcgtctatag atatcctctg
      481 cgtactttaa tctttctttt ttctttttcg gagacgttcc tgcattatag gctccaacag
      541 cctcccaccc tcttccatac atcatcataa actcatttaa taactttgct gcataatgta
      601 cagaaagaca tggatgacta attagcattt cctcagaata tcccatttct ctaagtcttt
      661 tagaatgaaa gtcatttatt tgcattatac cataatcttt acttccattg ttattaacat
      721 tgactgcaaa tttattaaat ccggattctt tttccgcaat cgcttttaac aaacttgatg
      781 gaatattgta cctttcacca gccttatccc aacaatcagc tctccctaaa tatgggataa
      841 aacacagcaa aataaatacg aaacgggaca ttaatacccc ttcattcttc gcgcaaattc
      901 atccttcagc caacgcacac gcgaaatata atattctgta ttaacatata catcctcgaa
      961 ttcatttatc ccggtaagat ctaatcgcgc aattagattt ccgccttcat catcagtagc
     1021 taagcatatc ttttctgagt aatttaggct caacgcatat ataagatcat ttatattttc
     1081 aggcaatgca caaaaagggc ataataaatt tattgaatct ccttcttttt caatatatat
     1141 tagcacatca tcatcaagca tgattgcttg atcatcgata tcaattaaag ggatacccaa
     1201 ggctcggcaa ataacatctg ctaaatcttc catatattcc tcttatacaa atgacgaata
     1261 ccctttcacc atattccata tttttgagtc ccctattctt tcagaataag atagctctaa
     1321 cgaagatagc ggcaattttt tcatgacttt atttccgggc acaccagtat tcatctcttg
     1381 gatt
//
LOCUS       DAZSBF010000444         1379 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZSBF010000444 DAZSBF010000000
VERSION     DAZSBF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.451"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(208..1122)
                     /locus_tag="ACHXWD_004507"
     CDS             complement(208..1122)
                     /locus_tag="ACHXWD_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7623160.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAADGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            complement(1226..>1379)
                     /locus_tag="ACHXWD_004508"
     CDS             complement(1226..>1379)
                     /locus_tag="ACHXWD_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HFR7623161.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
BASE COUNT          392 a          303 c          274 g          410 t
ORIGIN      
        1 tattatcgtt aaacaataga ttaaatacga taagttaagc atcatctctc atagtgttta
       61 aattaacata tttttcaata taaaaaataa gactctggct tcaattgtgc gcggattttc
      121 ttacaggtgt aggataatag ccttcacaaa ataattatct gcgcaatttt cgtgaatgga
      181 gcgcatagac taaggtcaaa atcacaatta ttcatagata aaagtgacac caatgactga
      241 ctggacggtt ccggctgaaa ccttgtttcc tgtttgtcca taggttgcgc ttaaacccag
      301 aggtactttg cttttattta ctgtccccag ggagacattt tctcccgtag ccagtatttt
      361 tccattacgc attagagtga cacccactcc cgatgctttt gttgcatcag gtgctgtgtt
      421 agcaaacact tgtcggctac tgtcagtggt ggcacccgaa agataaaatg agagcttttg
      481 ttcgcttgag caatatacgc caaggggtat ttctgcgctt ccaggaaagt ccgggagatc
      541 cacagtgaca ttacgactat caacggtgca gccgcctgtt ggcataacaa cactgttatt
      601 tgaaataata ttccacgtaa aattacgcgg atttccgctt cctaatgttg caattttata
      661 catatgaata cgggcaatga cttcccccgc ttttatgaca actccatcgg ccgccccaac
      721 tggggtaata tagagtttta acggtaatgg catcggtgtc ttatcaccaa tatccaatac
      781 gttggtattt gtcgtcaacg ggaaggggta ggtcacatta ttccagtaca gcgatccttt
      841 atatgactgt agggagcctg cgaaagctga gccttgtacc aggtttatat gatcagtgtc
      901 gtaccagccg ccgtaatcat tccagcaact tatatgctga gacaagtcta caaccagatt
      961 ctggcccggc tgtattacag ggtcgagatt aacataaaca gaggttgtac ctgctccaat
     1021 acttgacccg ccatcgacat tacaactgaa ggcaaaaact ttgcctgcca ttaatagaag
     1081 ataaataccg aacaggacct ttatactgat tgttttaccc atgatatatc ctaaggttaa
     1141 aaattgattt aaaaagaagg ctaagaaaat cgaatcgacg ttattgccag gtgtagatca
     1201 cattgattag cgcctcgatc gttccctagc ttgcgtttcc attcaccgtg atagctcttg
     1261 ccttaagtgg aaactgtgca ttacgagtga tctcatcaac aataaccgtt ttgctatcgc
     1321 cattttttaa cgcagcatcc tggtcatccc tcagctctat ctgaatattt tcggcagta
//
LOCUS       DAZSBF010000445         1376 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZSBF010000445 DAZSBF010000000
VERSION     DAZSBF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.452"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..983
                     /locus_tag="ACHXWD_004509"
     CDS             <1..983
                     /locus_tag="ACHXWD_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR7623162.1"
                     /translation="VVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="ACHXWD_004510"
     CDS             998..>1376
                     /locus_tag="ACHXWD_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR7623163.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAZSBF010000446         1371 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZSBF010000446 DAZSBF010000000
VERSION     DAZSBF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1371
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.453"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            102..734
                     /locus_tag="ACHXWD_004511"
     CDS             102..734
                     /locus_tag="ACHXWD_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR7623164.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1068..>1371
                     /locus_tag="ACHXWD_004512"
     CDS             1068..>1371
                     /locus_tag="ACHXWD_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7623165.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          449 a          250 c          256 g          416 t
ORIGIN      
        1 aatcgcaatt tttcccagca aaattacaaa atcaagatgt attgacatca tcaatcacca
       61 atgaaattat gcaattatat acggataggg aggttcttaa catggggctt tgtagtcgtt
      121 ataaaagtct tacatgcaat agttgctcaa tgcattgcca aataatgcca gaagagtcac
      181 cgcgtttgca gtattgcgca aactcgtgtt tttgtatgtg gcccgaagaa agttcatatt
      241 ttaatcgtgg tgtggtggaa ggtatcttaa caaaaaacca caatgcgaga ttaagcggat
      301 atatttttgt ggatttttca gtaagttttt tacgtctatt ccttgaaaaa gactggattg
      361 actatcttgc aagtactgac atggggatag ttttagtcag cgaccgcaat atgcaatcgt
      421 tagctaacta ctggcgaaaa cacaacaccg caatatctgc tgtcatatat aatgatgatg
      481 gtcttgatgt tgccaatgaa aagatcagac aactatttat tggtcgttat ttatcattta
      541 ccagaggtaa cacattaact cagatggaat tcaccataat ggggtatatg gtttctggtt
      601 ataatccata tcagattgct gaagtgctgg atatggatat ccgtagcatc tacgcgtaca
      661 agcaacgaat cgaaaagaga atgggtggta aaataaacga attatttatt cgttcacatt
      721 cggtccaaca ttgatactca acgaccagcc agaatcatac tctgttcata cgggcatcta
      781 ttgaaatgcc cgtattttat aaataagcag taaccccaag agtaaacaac atagtattta
      841 aaaccatcac acgaaaaatt cagaagcatt acgaaatatg aatttacaaa caatagtggc
      901 ataaatgtta accatgttaa tttacgtaaa gttttacgtt gcaacattaa agcctcattt
      961 caatcatcat gataaatata aaattaatat atatttatgc cgtaaatacc aatatactta
     1021 gcaaactatg tgatctccat ttcgattgat ttagtgttta ttgacgtatg tactggatta
     1081 ttaacgataa tatcgagttc tggcctgagc accgaaaatt aatatcggta cataacgccg
     1141 atcttaacgt cgttctgaca acgccagcca gtcgatgttt atcacttcta cttgaagctt
     1201 ttcctgatgt ggttgcacaa caagattttt tcaccagagt ctgggaagaa gaaggtatgc
     1261 gtgtgcctac taacacgtta tatcagaaca tatccattat cagacgcgga tttcgcgctg
     1321 ttggtgatac tacccactcg ctaattgcaa ccgtgccgag agaggattca a
//
LOCUS       DAZSBF010000447         1362 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZSBF010000447 DAZSBF010000000
VERSION     DAZSBF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.454"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..997
                     /locus_tag="ACHXWD_004513"
     CDS             <1..997
                     /locus_tag="ACHXWD_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR7623166.1"
                     /translation="YNFNIEKTDFVVIHIPDQIGDAMAIFPVIRALELHKIKHLLIVT
                     STINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAKNITQQYGTPDLCIEGMRKK
                     NLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQKLRAPVPMTWAFMMREAGF
                     PAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQERTFSLSIAENLIAKIQSE
                     TDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRSAAIIKDAYIAITPDTSILH
                     MASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT          437 a          258 c          286 g          381 t
ORIGIN      
        1 ttataatttc aatattgaaa aaacagactt tgttgtcatc catattcccg atcagattgg
       61 cgatgctatg gccatctttc ctgttattcg ggcgcttgaa ttgcataaaa ttaagcatct
      121 tttaattgta acgtcgacaa ttaatttaga agtctttaat gcgcttaaac ttgaacagac
      181 taaattaaca ttagtcacaa tgactatgca ggatcacgca acattaaaag aaataaaaga
      241 tttagcaaag aacataacac agcaatacgg tacgccggat ctttgtattg aggggatgcg
      301 taaaaagaac ctgaaaacga tgttatttat cagtcagttg aaagcaaaaa cgaattttca
      361 ggttgttggt ataaccatga attgctactc ccctttgtgc aagaacgcgt cccgtatgga
      421 tcagaaactc cgggctcccg tacctatgac atgggcattt atgatgcgtg aggcgggttt
      481 tccagcagtc aggccaatat atgaattgcc actaagtgag gatgtactcg atgaggtgcg
      541 cgaggaaatg cgatcgttag gatcttacat tgcgttcaat ttagaaggta gctcgcagga
      601 acgtacattt tcattatcga ttgcagaaaa tctaatagca aaaattcaaa gtgaaacaga
      661 tataccaata gtgatcgttc atggacccaa aggtgaagat aaagccaggg cattagtgga
      721 ttgttataat aatgtctacc gtttatcctt accaccctcg attaaacgtt cagcagcaat
      781 cataaaagat gcttatatcg caataactcc tgacacctca atattacata tggcaagtgc
      841 ctataatacc cctgttgttg caatttatgc tgattacaaa acgcgatggc ccgcaatggc
      901 cgatgtctcg gagtcagtcg tcgttgggca aaaaattgac aatataagtc tggatgaatt
      961 cgcaaaggca ttaaaaagtg ttttggcgag aatatgagac agtacttatg catagtaatg
     1021 ccgatctgtt atgaacggtg gtgtcatcaa tactctgagg ctggtcaatc atcacagaaa
     1081 accagccttt gaatcaaagc agtactcacc ggatacgatc atagtcacat cgaacgcaat
     1141 tctggcggtg ccgcattttt atttattcat tttctgtgag agaagggcaa gctctataga
     1201 gggaatgtaa aaaagagagc tccgaatgga gcactcttta agaaattata acggaaaatc
     1261 agaggttttt ataacatgtc gcataagcaa acccaagctt ttcaaatacg ctttgatcac
     1321 ctgttgccag ggaaactacg tattgttcgc ttgagtttgc gg
//
LOCUS       DAZSBF010000448         1354 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZSBF010000448 DAZSBF010000000
VERSION     DAZSBF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.455"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..511)
                     /gene="mxiJ"
                     /locus_tag="ACHXWD_004514"
     CDS             complement(<1..511)
                     /gene="mxiJ"
                     /locus_tag="ACHXWD_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFR7623167.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFL"
     gene            complement(517..810)
                     /gene="mxiI"
                     /locus_tag="ACHXWD_004515"
     CDS             complement(517..810)
                     /gene="mxiI"
                     /locus_tag="ACHXWD_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFR7623168.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(823..1074)
                     /gene="mxiH"
                     /locus_tag="ACHXWD_004516"
     CDS             complement(823..1074)
                     /gene="mxiH"
                     /locus_tag="ACHXWD_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFR7623169.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(1082..>1354)
                     /locus_tag="ACHXWD_004517"
     CDS             complement(1082..>1354)
                     /locus_tag="ACHXWD_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFR7623170.1"
                     /translation="KGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHK
                     NIYGDQYIEFSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
BASE COUNT          420 a          266 c          188 g          480 t
ORIGIN      
        1 tcaaaaatcg cttaatatta ctaactaata gttcagactc tttcggtgag tcatatatag
       61 cgattactga tatatgcatc ggttttgaag atatattttt ttcttcaaga tcatagctaa
      121 catgtatttt tgccgaaata acaccaccaa tagaaactaa agactgttcc agccgttgct
      181 caatagcact atataaacgg gccttttcag ctcttggaga agacactaat gaatctgtag
      241 gaaacatttg tgagatatca actctctccg gatttggcaa atcgtacatg cgcatcaaat
      301 caactgccga tgcaaatgtc cccttttcga cttgtaccga gatcccctgt ttacctccat
      361 caacttttct agcagtaata ttatggcgtt ctagcacaga tattatttca tttgcctgtc
      421 tttgagataa attagaaatt aactcttcac gttgctcaca tccaatcaac atcagcaaca
      481 agaataaaat aaaaccttta tacctaatca tataaattaa gactttaata aagtttcaac
      541 agccgaaact gtttttcttg ctaacgtgcc agctaatgat actcctattg aataatttga
      601 gagcgtcgtc tgtaacttgg ccaaagattc tgggtttaat gattctggat tgcttaccat
      661 ctccattatt tgtgatactt gaatatctgt atccatctta atatcactat atttagccga
      721 cacgacatct tccagacttg atatctcttg agattgaaaa tcactggctt tgataatatc
      781 aacctgattg actggataaa tgtaattcat gaatgctccc tattatctga agttttgaat
      841 aattgcagca tcaacatcct taatcacttt cactgtattg gattgcgcgt tcctatataa
      901 tgtatattca gataatttac tttggtattc agccagcaac tgtggattcg aaggattttt
      961 agctaattta tctagtgcca atgttagttc accttgtaat gtttgagttc catcatcaaa
     1021 agtttcagat aatgaactca gtgtccaatc atcattcggt actgtaacac tcattttatc
     1081 ctcacttatt tttatctaaa aaaaaccagt gtttatcatt caacattaca taactgtctt
     1141 tgctgttaag atatgattta cctttaaaat catcatctat caaaagtaca gaaaactcaa
     1201 tatactgatc tccatatata tttttatgtt cagaaatcag tctattaatg agctcaagct
     1261 tttcatctgt cagttcttct ctgacagaat atgtaacttt attattttca caaatctctt
     1321 tatactggac atttaccttt gtgaaaatac cttt
//
LOCUS       DAZSBF010000449         1349 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZSBF010000449 DAZSBF010000000
VERSION     DAZSBF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.456"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..184)
                     /locus_tag="ACHXWD_004518"
     CDS             complement(<1..184)
                     /locus_tag="ACHXWD_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR7623171.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
     gene            complement(229..1215)
                     /locus_tag="ACHXWD_004519"
     CDS             complement(229..1215)
                     /locus_tag="ACHXWD_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR7623172.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          372 a          298 c          272 g          407 t
ORIGIN      
        1 cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt aatgtacttg
       61 gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc agagcaagca
      121 tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg cggttaaata
      181 tcattgtaag catcttggaa aatcagggcc agagactggc ccggaatatt attgagcagc
      241 gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt cgtcaaacag
      301 aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac cccccatagc
      361 tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt gataagcttc
      421 ggatttcttc gctgctgttt cgaggatttg tttctgttct ggggttaatt tattccatgt
      481 tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag aaaaaacttt
      541 ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg cgccatctac
      601 tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg gtgaaccacc
      661 catcagttca atcattttga tggtggtcgg acttggctga acacgaattt tcagcccttt
      721 aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac ccgctacata
      781 agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt taccaacttc
      841 accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca aattatagat
      901 agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca tatccagtgc
      961 gccattttgc aacaattcca tagtttctcg cgcattaccc atctggctgc tgggataaat
     1021 acggatcacc atattacctt ctgacagctg ttttacctct ttcgccagct cttcaaaact
     1081 ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca ctttttctgc
     1141 ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg agcgggtaaa
     1201 tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg atatctaatt
     1261 gtgcttatca gtgctttgtg cgacaaattg gttaacctat ctgacaactt gtctatgttt
     1321 atcacaactt gaaataaagt cagaaatta
//
LOCUS       DAZSBF010000450         1341 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZSBF010000450 DAZSBF010000000
VERSION     DAZSBF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.457"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHXWD_004520"
     CDS             <1..184
                     /locus_tag="ACHXWD_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623173.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHXWD_004521"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHXWD_004521"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR7623174.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZSBF010000451         1339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZSBF010000451 DAZSBF010000000
VERSION     DAZSBF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.458"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1339)
                     /locus_tag="ACHXWD_004522"
     CDS             complement(<1..>1339)
                     /locus_tag="ACHXWD_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR7623175.1"
                     /translation="YMNGHIGELDKLVPHLITEHKIYGRDRITPRTVSAEDGVIKKQN
                     NPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEKASDLCLELIDTQDDDGYIG
                     IYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESLLTSLTQAAQCIMAGYPKET
                     SHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYCLWLYEDYSAGCVSEQDLQY
                     KNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALDIFLSQLKYYLTPSGAPIGD
                     EWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGLADDAEWLFYNASFGMQHPI
                     LHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEAAVCCVPNTARTIPAFVENM
                     FQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDLSVKCLIEPESSVRFA"
BASE COUNT          404 a          273 c          248 g          414 t
ORIGIN      
        1 agcaaaacga acagatgact caggctcaat gagacatttt acgcttaaat catgagggta
       61 ttcagttaat tgtgtaattt taattgcaac attattaaat gtgccataaa actcacacgg
      121 gccataaaat aatgcagtaa aaccattgtt attttcttga aacatatttt cgacaaaagc
      181 cggtatggtt cttgctgtat tcggtacgca gcagacagca gcctcctcat gagtagggga
      241 gtatttatat ctggtattaa actgattact gtcaggatgt ttcctttcat ttgcttcata
      301 acaattatca gtcttacagt acattatcgc gtgaagtatg ggatgctgca tgccaaaact
      361 agcattatag aatagccact cagcatcatc ggccagacca atattagtgg tcagttttat
      421 taacataaca taagtgctaa atagttcatg aacagaacaa tattcatagc ctgtataagt
      481 ggcattagct gtgcgtcctg taatccactc atcaccaatg ggagcgccag atggtgtaag
      541 ataatatttc aactgggata aaaaaatatc taatgcagtt tgatactgcg gccgttggct
      601 cgctgcgatg gctaatgccc taatatgctc atacgtatgt acgccatgac ctttaagaag
      661 gtaaccaggg tctatgacat ttttatattg cagatcttgc tccgacacgc atcctgcgga
      721 ataatcttca tacagccaca agcaatatga gagatattca atgttattgg ttatttcata
      781 aaggcggtag aaaacatctg ttattgttaa tccgtggcta tgtccagcaa aaccctgttc
      841 cacaacgaaa ggatgtgacg tttctttagg gtagccagcc ataatgcatt gtgcggcttg
      901 ggtaagagaa gttaataaac tctcattacg ggtggactca taacatccaa gcacaaaacg
      961 caaggcagta gcttgtgccc agaactctcc gttttctgct ttacagttaa attttaatga
     1021 tgaatcataa attccaatgt agccatcatc atcttgcgta tcgataagtt ccaaacataa
     1081 atcgctcgct ttttcctgcc attctttatt atctaacaag taggcactgc ggcaatatcc
     1141 atccttccaa ttcgattgag tttcactatc ccaccaataa tattgcatca tgttatcatc
     1201 gggattattc tgttttttaa taacgccatc ttcagcagat acagtgcgtg gagttatacg
     1261 atctcttcca tatattttat gctccgtaat aagatgggga actaatttat ccagttctcc
     1321 tatatgaccg ttcatatac
//
LOCUS       DAZSBF010000452         1339 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZSBF010000452 DAZSBF010000000
VERSION     DAZSBF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.459"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..637
                     /gene="erm"
                     /locus_tag="ACHXWD_004523"
     CDS             <1..637
                     /gene="erm"
                     /locus_tag="ACHXWD_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFR7623176.1"
                     /translation="EIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVT
                     LIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLD
                     IHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFV
                     SKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          477 a          239 c          223 g          400 t
ORIGIN      
        1 cgaaattgga acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt
       61 aacgtctatt gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa
      121 tactcgtgtc actttaattc accaagatat tctacagttt caattcccta acaaacagag
      181 gtataaaatt gttgggaata ttccttacca tttaagcaca caaattatta aaaaagtggt
      241 ttttgaaagc catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac
      301 cttggatatt caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt
      361 gcttaagctg ccagcggaat gctttcatcc taaaccaaga gtaaacagtg tcttaataaa
      421 acttacccgc cataccacag atgttccaga taaatattgg aagctatata cgtactttgt
      481 ttcaaaatgg gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc
      541 aatgaaacac gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat
      601 ttttaatagt tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat
      661 ttggaaagtt acacgttact aaagggaatg tagataaatt attaggtata ctactgacag
      721 cttccaagga gctaaagagg tccctagact agcaagaagt acacaagaag ccttaaagat
      781 tatagaaaag cttttccatg aaatgcttaa cattatttaa tgttaacatg tggctttgtg
      841 gtaactacca cgagtggtaa cttgtaacat gaaaaaagtt acaagttaca agtggtttat
      901 ttacgaacat aaaggctagg aaagcacttg ataagtatat caagtgcttt ttttccgttc
      961 gattttacta tccaagatac cgcttgacgt acctaatatt ttagtgtgct aagggctttg
     1021 ctttttctgg ttatagttcg ttcaaaaaag accgcaaggg taaatgcacg atcatagatc
     1081 gcccttgcag ctttttaatc tctccacttt tttataacca agcaaaccac caagcacact
     1141 gaaaaaataa ttaggaacta aagcgaagtg cgtatcttgg ataaatcttc actcgatttt
     1201 aaaagattaa gcacccagcc aaagcgccta aaaaaataat ttttttaaag aaaggaagta
     1261 atttaaatga ctacaatttt aagcgactac aataaatcta tcctaattga attactaaac
     1321 accaatcgcc aaattatcg
//
LOCUS       DAZSBF010000453         1333 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZSBF010000453 DAZSBF010000000
VERSION     DAZSBF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.460"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            39..665
                     /gene="ipgB1"
                     /locus_tag="ACHXWD_004524"
     CDS             39..665
                     /gene="ipgB1"
                     /locus_tag="ACHXWD_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFR7623177.1"
                     /translation="MQILNKILPQVEFAIPRPSFNSLSYNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDNVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSNMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            723..1190
                     /gene="ipgC"
                     /locus_tag="ACHXWD_004525"
     CDS             723..1190
                     /gene="ipgC"
                     /locus_tag="ACHXWD_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFR7623178.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            1196..>1333
                     /locus_tag="ACHXWD_004526"
     CDS             1196..>1333
                     /locus_tag="ACHXWD_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623179.1"
                     /translation="MHNVSTTTTGLSLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LT"
BASE COUNT          496 a          240 c          219 g          378 t
ORIGIN      
        1 acatcacttc agaagtgaac taacatatag ggggtatcat gcaaattcta aacaaaatac
       61 ttccacaggt tgaattcgcg attcctcgcc catcatttaa ttcgttaagt tacaataagc
      121 ttgtaaagaa aatattatct gtttttaact taaaacaacg ctttcctcag aagaattttg
      181 gatgcccggt gaatataaat aaaatcaggg acaacgtcat agacaaaata aaagatagta
      241 attcggggaa tcaactattt tgttggatga gccaagagcg aacctcttat gtctcttcaa
      301 tgataaacag aagtatagat gaaatggcca tacacaatgg agttgttttg acctcagata
      361 acaaaaaaaa catatttgca gctattgaaa aaaaattccc ggatataaaa cttgatgaaa
      421 aatcagcaca aacatctatc agtcatacag cactaaacga gattgcctca tctggcctca
      481 gagcaaagat tttaaaacgc tatagcagta acatggattt gtttaacaca caaatgaagg
      541 atcttacaaa tctagtatcc agttccgtct acgataaaat atttaatgaa tcaacaaaag
      601 ttttacaaat agaaataagc gcagaggttc taaaggccgt ataccgtcaa agcaatacaa
      661 attaaatctg tttatattaa atcttatact tattaaagta aagcagacaa aaaggagacc
      721 ttatgtcttt aaatatcact gaaaatgaaa gcatctctac tgcagtaatt gatgcaatta
      781 actctggcgc tacactgaaa gatattaatg caattcctga tgatatgatg gatgacattt
      841 attcatatgc ttatgacttt tacaacaaag gaagaataga ggaagctgaa gttttcttca
      901 gatttttatg tatatacgac ttttacaatg tagactacat tatgggactc gcagctattt
      961 atcagataaa agaacagttc caacaagcag cagaccttta tgctgtcgct tttgcattag
     1021 gaaaaaatga ctatacaccg gtattccata ctggacaatg ccagcttcgg ttgaaagccc
     1081 ccttgaaagc caaagagtgc ttcgaactcg taattcaaca cagcaatgat gaaaaattaa
     1141 aaataaaagc acaatcatac ttggacgcaa ttcaggatat caaggagtaa ttattatgca
     1201 taatgtaagt accacaacca ctggtttgtc tcttgccaaa atattggctt ccactgagct
     1261 tggagacaat actatccagg ctgcaaatga tgcagctaac aaattatttt ctcttacaat
     1321 tgctgatctt act
//
LOCUS       DAZSBF010000454         1330 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZSBF010000454 DAZSBF010000000
VERSION     DAZSBF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.461"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            323..>1330
                     /gene="shiA"
                     /locus_tag="ACHXWD_004527"
     CDS             323..>1330
                     /gene="shiA"
                     /locus_tag="ACHXWD_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR7623180.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT          284 a          321 c          328 g          397 t
ORIGIN      
        1 aaaaacatac ctaacctcgc ctccctactg gttataatgc aatgcagtct atcagactca
       61 tcagggtgcc attttgtgca tatgcggact tttatgtttc atatctctaa cctgtgggtc
      121 ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct ttcatcttcc atcgaatttt
      181 tcatgttgcc gctaatcagc cataaaatca tttgcagatg cgctctgtcg aggtagtctc
      241 ataaggttcg tttatagatc gacggcaatg tgagttacct tttccatact aattataaaa
      301 agacagtaca aacaggatca ttatggactc cacgctcatc tccactcgtc ccgatgaagg
      361 gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc agcttcgctg gtgccgtcgt
      421 cgactggtat gattttttac tctatggcat caccgccgca ctggtgttta atcgcgagtt
      481 tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca tttgctacct ttggcgtcgg
      541 attccttttc cgtccgctcg gcggtgtcat tttcggtcac tttggcgacc gactgggacg
      601 taagcgcatg ttaatgctga ccgtctggat gatgggcatc gcgacagcct tgattggtat
      661 tcttccttca ttctcgacca ttgggtggtg ggcacctatt ttactggtga cactgcgtgt
      721 cattcaggga tttgccgtcg gcggcgaatg gggaggcgcg gcgttgcttt ccgttgaaag
      781 tgcaccgaaa aataaaaaag ccttttacag tagcggtgta caagttggct acggtgtagg
      841 tttactgctt tcaaccggac tggtttcatt gatcagtatg atgacgactg acgaacagtt
      901 tttaagctgg ggctggcgca ttcctttcct gtttagcatc gtactggtac tgggagcatt
      961 gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa caacagcaac attatcaagc
     1021 tgccgcgaaa aaacgcatcc cggttatcga agcgctgtta cgacatcccg gtgctttcct
     1081 gaagattatt gcgctacgac tgtgcgaatt gctgacgatg tacatcgtta ctgcctttgc
     1141 acttaattat tcaacccaga atatggggct accgcgcgaa cttttcctta atattggttt
     1201 gctggtaggt ggattaagct gcctgacaat tccctgtttt gcctggcttg ccgatcgttt
     1261 tggtcgccgt agggtttata tcacaggtgc gttaatcgga acgttgagcg catttccttt
     1321 ctttatggcg
//
LOCUS       DAZSBF010000455         1326 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZSBF010000455 DAZSBF010000000
VERSION     DAZSBF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.462"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(339..605)
                     /gene="ospE2"
                     /locus_tag="ACHXWD_004528"
     CDS             complement(339..605)
                     /gene="ospE2"
                     /locus_tag="ACHXWD_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFR7623181.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(787..1185)
                     /locus_tag="ACHXWD_004529"
                     /pseudo
     CDS             complement(787..1185)
                     /locus_tag="ACHXWD_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          343 a          266 c          273 g          444 t
ORIGIN      
        1 aagccgtcta tgggaaggag tctgctgtta ttccagagaa tggtaattca gtcatatatt
       61 tctccatcta tgacttgtca ataccatcct gatatttgtg ggtcagcaat atattctact
      121 aaaaggtatt taagatcatt gcgtccctgc actcgaataa tggtgaagaa agcatgatga
      181 atatatcacg ataacgttca gtatgtatcg aggatagatg gagttactcc tctctgaaat
      241 agaattactg ctctactttt tctgtttcca gaaattacta gtaacaaacc ctgatatatt
      301 cttttgaaaa tggagcttaa acgagtctga aattaacttc agatattaga atgccgttgg
      361 ctttgttttt caacaattcc tacttcattt atctttgtca accattgcat ttctgtagca
      421 gtgaatttca acgttttcgt ccggttggca gcaggtattt gtaattcgta tatagctgct
      481 tttttattga aaacagtata tccatctgta gtaacagtag aagataataa tacaggattt
      541 gatacttcaa ggatgatatt ttcttgtttt tgtggaaggc aagggaatat agtttgtgta
      601 agcatattgc ttccttatgt tatattttag taaagaattt attttttaca ttatagtcgg
      661 ctaaatgaag tttactgtaa aaaaacgata cttttttcgt aagcgatgag atgtggagcc
      721 agaaaaatgg ctgcgttacg tcattgaact gtccttgacc cacgatattc agacagtcgt
      781 tgtatcttaa gttgacatta cccgctgacg tcgatattcc cttggtgaga gataacccaa
      841 cgcactatgc ggatggtttt cattgtaatg ctcgatcgcc actgcaaggt tatgcaatgc
      901 cgttcttggt ttcggcatga acgcgatgca gtcttccttc atcgttttca cgaaccgttc
      961 tgctattcca ttactctgag ggctactgac tgccgtcgtg cagggctcca gattcaactc
     1021 tctggcgaac tgccgtgttt catgagccgt ataagccgaa ccgttatcgg ccactggatt
     1081 ggccgttccg gtcctttatc gccatatcgc tgacatgaca tcctgcaccg ttgctttgtc
     1141 ataatctccc gtgcctgctg cccagtctat ggcttcacga tcgcaatatg gaacgtaacg
     1201 ctgtcatcgg gagaatcgtc aatgtggcat tcggttaacg ctgatatgac tttccgtgtc
     1261 aaaccggaat ctctaatttt gacgattttc tttttaggtt taattccctg agggcgacgt
     1321 agcagt
//
LOCUS       DAZSBF010000456         1321 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZSBF010000456 DAZSBF010000000
VERSION     DAZSBF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.463"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            100..288
                     /locus_tag="ACHXWD_004530"
     CDS             100..288
                     /locus_tag="ACHXWD_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623182.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQDTGG"
     gene            298..>1321
                     /locus_tag="ACHXWD_004531"
     CDS             298..>1321
                     /locus_tag="ACHXWD_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR7623183.1"
                     /translation="MLSAFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACG
                     TRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHL
                     VFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITT
                     ESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLS
                     FRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRA
                     LGMDKPE"
BASE COUNT          295 a          352 c          398 g          276 t
ORIGIN      
        1 ctgcaaattg aaaaccgccc ctgttcgtcc tgttgttgcc gcacgctccg cagaatatgt
       61 atgctctgag gacctgtttt attaaggact catcctctta tgttgacccg caaaagtata
      121 gataccgttc tgctctctgt tggtgctgag aagctctcac aacgtgaatg ggactggatg
      181 aagatgctca aacccatgga cccaccacct gcgatggtcg cggcctccat cctggagcgc
      241 cgtggcgaca cagcagccct tacccggcta caggataccg ggggttaatc cgtcctcatg
      301 ttgtccgctt ttactccccg cccgctgaaa cgtctgttca cggccaacca gtgctggacg
      361 tccttcctgg atgcgggcgg tctgcgcgat atcgaggttg aggctgtcac aaaaatgctg
      421 gcctgcggca cgcggatact gggagtgaag gaatacaact gcgataaacc tgagtgcccc
      481 cacgtcagat acgtcactaa ctcatgcggc agccgtgcct gcccgtcctg cggaaaaaag
      541 gccacagacc tgtggatagc gacacagctg aatcgtcttc ctgactgcga ctgggtacat
      601 ctggtcttca ccctgccgga cacgctgtgg ccggtgttcg aaagcaaccg gtggctgctg
      661 aatgacgtgt gccgtctggc ggtggagaat ctgctgtatg ccgcccggaa acgggggcag
      721 gaacccggta tcttctgcgc catccacacg tatggccgtc gtctcaactg gcatccgcat
      781 gtacatgtgt ctgtaacctg tggatgtctg aataagcatg gtcagtggaa aaagctgagc
      841 ttcctgaaag acgcgatgcg ttcgcggtgg atgtggaata tgcggcaacg gcttctgaaa
      901 gcgtggtcag aggggctggc gatgccggag tcgttgtcac atatcacgac ggaatcacag
      961 tggagaagcc tggtgctgaa agccggagga aaatactggc atgtgtacat gtcgaaaaaa
     1021 acggccggcg ggaggaatac ggcgcgctac ctgggtcgtt atctgaagaa gccgccgata
     1081 gcggcctccc ggctggcaca ttacaacgga ggggcgagcc tgagcttccg ttacctggac
     1141 cacaaaacgg gagaaacggc gacggaaacg ctgacacagc gtgagctggt cgcgaggctg
     1201 aaacagcaca tcccggagaa gttttttaag atggtgaggt acttcgggtt ccttgccaac
     1261 cgggtgtgtg gagagaagct gccgcaggtg taccgtgctc tggggatgga taaaccggaa
     1321 c
//
LOCUS       DAZSBF010000457         1298 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZSBF010000457 DAZSBF010000000
VERSION     DAZSBF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.465"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>1298
                     /locus_tag="ACHXWD_004532"
     CDS             <1..>1298
                     /locus_tag="ACHXWD_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFR7623184.1"
                     /translation="ARRVADLLNIVLPDNATSYTLRDIIEALPKDSNAAWKLTSNAMS
                     MMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWAAPEYRSEQGK
                     IQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQLLKREEELRR
                     KEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLLLHNR
                     NGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSATFEQLLT
                     QVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTLMPVMSS
                     VDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYAG
                     YGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEK"
BASE COUNT          329 a          333 c          370 g          266 t
ORIGIN      
        1 gcccgccgtg tggctgacct tctgaatatc gtgcttcctg acaacgcaac ttcgtacaca
       61 ctacgtgaca tcatcgaagc gttgccgaag gacagtaatg cggcatggaa actcaccagt
      121 aatgccatgt cgatgatggc agatatgcag gcgacgcgca gacgctggga agagctgaac
      181 accgaacgaa aggaagcgat gagccatgct gccagcctga aaaaatcatc gccgttttcg
      241 tccggttttt cccgtattcc cctgatgcaa tgggcggcac cggaataccg tagcgaacag
      301 ggaaaaatcc agtcgctgag ccagcaaatg gaaaaattgc gacagcatta ccggaaggtg
      361 gagaaacagg acattcccgc gagtcagacg gcatttgaaa aacagtggca gcagtggggg
      421 gcatccggcc ttgcagctct cagagtccag ttgcttaaac gtgaagagga attgcgccgg
      481 aaggagcagg aggatacaga acgtagtcgc gcggagcgac gagcggaaca gctcaggaaa
      541 aatgacaatg ccgttatgga ttcaggggta ctgagcgcag tcgttacggg gtatggcgag
      601 gcccccatgc ctggtaacgg ggaaatgacc tgttaccttc tgcttcacaa ccggaacgga
      661 gaatacacgc tctgggggaa cgagctggag aaataccgca ccagaatctt tgagcgtgtt
      721 gatttaatgc gtgacaggag tggttatatc tgtgaccgaa gcgaaatcgg tcaacatcca
      781 ccactgcaac gtgtttattc ctctgcgacc tttgagcaac ttctgacgca ggtttgccag
      841 acatggccgc aatatacccg caatctccgc cagccgaaga catggccgga gtctttctgt
      901 cttggagaag accgccagcc agccatgcca tcactggcag caaggaaggt ggattttacg
      961 cagggacgtc tgctaccgac gctgatgccg gtaatgagtt ctgtcgaccg ggaaacccgc
     1021 cagcttcagc ttttgctggt catgggcgtg gatgactctc tgggcggtgt tgtccggctt
     1081 aatggtacgc tgtatcccgc gtttgccgtt ccctcagccg ataacagcca gctggtcatc
     1141 agtgcactga ccgataaggg gttacggtat gcagggtatg gtgttgccgt aaatcacgat
     1201 gcagacagcc acatatcacc tgcgccggaa cttatggagt ttcatctgaa aacccgtgag
     1261 gcaccgcttt ttgctgccgt caacacacca gaaaaaca
//
LOCUS       DAZSBF010000458         1295 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZSBF010000458 DAZSBF010000000
VERSION     DAZSBF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.466"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            73..1173
                     /locus_tag="ACHXWD_004533"
     CDS             73..1173
                     /locus_tag="ACHXWD_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR7623185.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1198..>1295
                     /locus_tag="ACHXWD_004534"
     CDS             1198..>1295
                     /locus_tag="ACHXWD_004534"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HFR7623186.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
BASE COUNT          372 a          279 c          332 g          312 t
ORIGIN      
        1 gcaagcggtt aattcgttgt gtatttaatt atacaatgat tccggtgttc tggaatttat
       61 ttagaggaat ctatgcgagg gaaaaaacgc attgggttat tgtttttgct gatagcggtt
      121 gtggttggtg gcggcgggtt attgctggcg caaaaagcct tacataaaac gtcggataca
      181 gcattttgcc tttcctgcca ctcgatgagt aaatcttttg aggaatatca gggaactgtc
      241 cacttttcga accagaaagg gatacgtgcg gaatgtgcag attgccatat tccaaagtca
      301 gggatggatt atttatttgc taaattaaaa gcatctaaag atatttatca tgaatttgtt
      361 agcggcaaaa tagacagtga cgataagttc gaaactcatc gccaggaaat ggccgaaaca
      421 gtatggaaag aattaaaagc aactgactct gcaacgtgcc gtagttgcca ttcttttgat
      481 gccatggata ttgcctcgca aagtgaatct gcgcagaaaa tgcataacaa agcacaaaag
      541 ggcggcgaaa cctgtatcga ttgtcataaa ggcattgccc attttccgcc agaaataaaa
      601 atggatgaca acgcggcgca tgagctggaa agtcaggccg ctacttcagt gactaatggc
      661 gcacatattt atcctttcaa aacttctcgc ataggcgagc tggctaccgt gaatcctggt
      721 accgatctca ccgtcgttga tgccagtggc aaacagccga tcgttctgtt gcagggttat
      781 caaatgcagg gcagtgaaaa cacgctctac ctggcggcag gtcaacggct ggcgctagcc
      841 acattaagtg aagaaggtat caaggcgctc acggtaaacg gggaatggca ggctgacgaa
      901 tacggcaatc aatggcgtca ggcgtcttta cagggtgcgc ttaccgatcc cggattagcg
      961 gaccgtaaac cgctatggca atacgctgaa aaacttgacg atacctattg cgctggttgt
     1021 catgccccta ttgccgccga ccattacacc gtcaatgcgt ggccgtccat tgccaaagga
     1081 atgggggcac gaaccagcat gagcgaaaac gaactggaca ttttaacgcg gtatttccag
     1141 tacaacgcca aagatattac cgagaaacag tgatcctcgt tgatcaggga ggaagttatg
     1201 acattaacaa gacgtgaatt tattaaacac agcggtattg ccgctggagc gcaggtggtg
     1261 acatcggcag caccgctccc cgcatgggca gaaga
//
LOCUS       DAZSBF010000459         1294 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZSBF010000459 DAZSBF010000000
VERSION     DAZSBF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.467"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..1112
                     /locus_tag="ACHXWD_004535"
                     /pseudo
     CDS             1..1112
                     /locus_tag="ACHXWD_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1090..>1294
                     /locus_tag="ACHXWD_004536"
     CDS             1090..>1294
                     /locus_tag="ACHXWD_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623187.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAZSBF010000460         1264 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZSBF010000460 DAZSBF010000000
VERSION     DAZSBF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.468"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(23..>1264)
                     /locus_tag="ACHXWD_004537"
     CDS             complement(23..>1264)
                     /locus_tag="ACHXWD_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7623188.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYPTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          333 a          298 c          228 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtcg ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAZSBF010000461         1253 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZSBF010000461 DAZSBF010000000
VERSION     DAZSBF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.469"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..449
                     /locus_tag="ACHXWD_004538"
     CDS             <1..449
                     /locus_tag="ACHXWD_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFR7623189.1"
                     /translation="IPSFLLKASEADLVAFLIPHRANTSYWLRHIYSNNHCHEIRILH
                     RAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCADTLLPLTIINRGGLR
                     GRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQRLS"
     gene            813..1202
                     /locus_tag="ACHXWD_004539"
     CDS             813..1202
                     /locus_tag="ACHXWD_004539"
                     /inference="COORDINATES: protein motif:HMM:NF025107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623190.1"
                     /translation="MSKYNPNQFYKFDVRISQLSEEINAAARLIYMVMVHSCDFYEEK
                     YKSYSPSIDHLAYMAGVSKRTAIRAIETLEKHGLIQVQKNQGRQSTYRVFTLDDKPEL
                     LVRPTSQEVAASVRQNDTTTSDKMSWG"
BASE COUNT          398 a          253 c          245 g          357 t
ORIGIN      
        1 atattccgtc gttcctctta aaagcgtctg aagcggattt agtagctttc ctgataccgc
       61 atagagccaa caccagctac tggcttaggc acatttacag caataaccac tgtcatgaaa
      121 tccgtattct tcatcgtgca gttaaatacc ttccaccagc agggcataac aggcttacta
      181 ttaggtcacc gtttccaagt gcagtcgttg tttttaaaaa agaaccacgt aagcatgaaa
      241 ttacacagat ggtttgctgt gctgatacat tgctaccgtt aaccatcatt aaccgtggtg
      301 gactaagagg aagacccacc atttacccac ctgagacgct cgacagcttc ataaaactat
      361 acaggcaagg aaaacccatt aagtacattg cagatgctct cagaatgcct ctgagcacgt
      421 catacaggat tgctcaaagg ttgagctaaa attctcataa atatggtcat ttacaagaca
      481 ggtgattcag atgttaagaa gtatccctgt gaacaataaa tgaccaatca gtaatcattc
      541 tgtgaaataa ttgttgcaat ttataatcgc atctgttata gtttctatat gggctgataa
      601 aaaagttcat gtcttgaagt ggtaacactt ggggaggtgt cagatggttg gcgtatatta
      661 acctctcatt cagggtatac ctcagcgttt taatcatcgt actgtttcag gcatggactt
      721 ctttatcagt ccatcaatcc cccagatatt ttaatcacac aggctaatcg gttttatccg
      781 gttggccttt tcacgtatga ggtaatttat gaatgtctaa atataaccca aatcagtttt
      841 ataaatttga tgtgcgaatc tctcaacttt cagaagaaat taacgcagca gcaagattga
      901 tttatatggt catggtccat agctgtgact tctatgaaga gaaatacaaa tcatattcac
      961 caagtattga ccatctcgct tatatggctg gtgtatcaaa gagaacagca atcagagcca
     1021 ttgagacact tgagaaacac ggtttaattc aggtgcaaaa gaatcagggg cgtcaaagca
     1081 cttatcgtgt attcacactg gatgataagc cggaattact tgtaagacct acatcacagg
     1141 aagttgcagc ctcagtaaga caaaatgaca ccactaccag tgacaaaatg tcatgggggt
     1201 agtgacaaaa tgtcacccgt gttagagttt agataagtta ttagataaaa cat
//
LOCUS       DAZSBF010000462         1243 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZSBF010000462 DAZSBF010000000
VERSION     DAZSBF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.470"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..689
                     /locus_tag="ACHXWD_004540"
     CDS             <1..689
                     /locus_tag="ACHXWD_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR7623191.1"
                     /translation="SQKDVFMTYRTTGLATYRNYDFFGLIEGLNFAAQYQGKNERTDN
                     SHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTNAQERAAANPLNASGKNAE
                     LWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSFEVVAQYQFDFGLRPSLAY
                     LKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKINLIDESDFTRAVDIRTDN
                     IVATGITYQF"
     gene            853..>1243
                     /locus_tag="ACHXWD_004541"
     CDS             853..>1243
                     /locus_tag="ACHXWD_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7623192.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKESKLEN"
BASE COUNT          382 a          267 c          242 g          352 t
ORIGIN      
        1 ggagtcagaa agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact
       61 acgatttctt tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg
      121 aacgtactga caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt
      181 tcggtatctc ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat
      241 ccgatcggac aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga
      301 atgcagaact atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta
      361 attacgctga aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac
      421 aaagttttga agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt
      481 acctgaaatc gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata
      541 tcgacgttgg tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa
      601 tcaacctgat tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg
      661 tcgctacggg cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct
      721 ggtttttgat ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat
      781 ttctggtcat tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat
      841 atttattgat gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt
      901 tatcaactta ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga
      961 ctagaacaat ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag
     1021 cgaattaaag aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg
     1081 agtgaattac cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta
     1141 acttgtcttg acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa
     1201 attaacacga taacctttcc aaaagaatca aaactggaaa aca
//
LOCUS       DAZSBF010000463         1236 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZSBF010000463 DAZSBF010000000
VERSION     DAZSBF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.471"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(413..1132)
                     /locus_tag="ACHXWD_004542"
     CDS             complement(413..1132)
                     /locus_tag="ACHXWD_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFR7623193.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPNYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          388 a          246 c          228 g          374 t
ORIGIN      
        1 gttataatac tattagattg cagttactaa tagtaaaagc ctccttacaa ggaggtagct
       61 tattcagaat gctcctataa gatagcgagc tcgtcaacga tacagatgat ctgattatat
      121 tgtgcaaaag gagaacagga aaataggcac tggaaaagat gaaggcctga tgggagcatt
      181 aactaatgca aaatccgaca ggttaatatc ccgaaaagca atcaaacgac tcaaacagga
      241 gatgcacgct gaagtggtgg acaactgttc ggatttcaga tgagtagctc agcagtttat
      301 gagccgaatg ctctgtcacg ggcaggaatc aggctgcata cgcagtgcca aatctaatcg
      361 tagttattct aaccagtttt caagctacaa ggtggtgtag ctggcatctt ctctactcta
      421 tcatcaaacg ataaaatggt tcctctcgta acatttgctc ttgcctttca cttccatctc
      481 gatcgcttcg tagttcatta cgatagctga catatttcca gtcttcaggt ctaacatctg
      541 acgccggata ctccccggga gcgattttgc ttcttaatag attagactcg agacacatta
      601 taaattgtcg tattttatga agtaataaag cactatattg cgagcattcc tgatctgatt
      661 ttacatatag cgtaaactga gctcctatcc ccacacgaga ttgctgagaa actcgattca
      721 tatcagttat cttccattta tctataggac tgtcctctga aaataataaa ccagacaaaa
      781 tttggaatgc cagaggaact tgctccctag ctatactaat atgaaacttg tccccaacaa
      841 aatccccctt actctgagga ttttcacgac atgcatgaat aaacacatct ccactatggt
      901 tcacaatctg gaatccttgg tacatgacag aaaaaaattg acatatatca gagtaatttg
      961 gcacgttcga tgctactttg aaatagtacg ctgggtattg ccgtatttgg ttgtacaata
     1021 tattgaagtt atttttcaat ctttcattcg cagaaagcat ctgcggtatt tcagacctca
     1081 caacattcaa agaatctaaa tttagtttta gacagggctt ttttatgggc atagaaaacg
     1141 tcctctataa aatagatata ttatcttata ttgattgtct atcatctttt ataaaacgat
     1201 acattatatg taatgtatgt aagcgtaagg gtcttc
//
LOCUS       DAZSBF010000464         1234 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZSBF010000464 DAZSBF010000000
VERSION     DAZSBF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.472"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHXWD_004543"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHXWD_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          353 a          263 c          270 g          348 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atca
//
LOCUS       DAZSBF010000465         1228 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZSBF010000465 DAZSBF010000000
VERSION     DAZSBF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.473"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /gene="ipaH4.5"
                     /locus_tag="ACHXWD_004544"
     CDS             complement(<1..784)
                     /gene="ipaH4.5"
                     /locus_tag="ACHXWD_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFR7623194.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTR"
BASE COUNT          349 a          222 c          267 g          390 t
ORIGIN      
        1 cgcgagtcga caatggattc cctgaaatgt taacattcgt acaaatgggc aatgtgatta
       61 tattttcggg taatgaagtt agtaggttat tggaaacatc cagtgccaat agttcttgcg
      121 gtaatctgga taatctgcgt agttgatttt cacctacctt caaatatttt aagcgctgag
      181 gaaattctgg tagttcctgt agtctgttgc cggaaacaaa gagttcttgt agtctatgag
      241 gcaattcagg caagacagta aggcgattac cttcaatacg taatattgtt agcgttgatg
      301 gaagctctgg taacgtctcc agactattaa aacgtgccga gagtgattgt aaatatggtg
      361 gtaatgatgg cagacatgac aaatttcgat tgaatgaaat attaagctta atcagttgag
      421 atggaagtgt cgggagtata ttcaagttgt taccatttac atgaagttct gtcaaaagcg
      481 gagaattttc aggtagggag attaactcat tgttggaaat attaagttca cgtatatgca
      541 aagggagtgg tggtaaagaa cgtagtttca gtaagcttaa attgagaaca ggctcccggg
      601 tatccagaca tattttcaat aatcgaaccg cctggatgcg attttctgtt gttgttcctt
      661 ccctagccca gtcatcccag atgcggtggt aatctctggt acactgttct tctaccgata
      721 aacgagatat acatgataag ccacaaaggg aacgaaaaaa agaatgattg ttgatcggtt
      781 tcatttatta acccctcact attcatatct tggtctatta atgcaaataa aacgagaaag
      841 tctttaaaaa aacaatccta gagctttatc ggcgttttca ttccagctgg aggaatatgt
      901 tcctgtcttt ccagtaaccg tcgattgtta tatcagtcta cccatgttaa tgtgaccagt
      961 tctactttca tccagtttgg attgatcttt atcccctata ttttcaaaca atttttatca
     1021 tttaactcat tactgtctcg atattcccat agcaaacgat ctggttggtt agcccctcaa
     1081 aaaagtcagt tgcacgccgt aaatccctgc cggtattgag cgaggattta cgggttgttg
     1141 ttaaggtccg taatcgggtc acagtttttc cggagtcaat ccggtctgcg gtttatgctt
     1201 atgcgacgtg attatgaatg gtgcagtc
//
LOCUS       DAZSBF010000466         1225 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZSBF010000466 DAZSBF010000000
VERSION     DAZSBF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.474"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHXWD_004545"
     CDS             complement(<1..815)
                     /locus_tag="ACHXWD_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7623195.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHXWD_004546"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHXWD_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHXWD_004547"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHXWD_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          340 a          211 c          329 g          345 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgc
//
LOCUS       DAZSBF010000467         1220 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZSBF010000467 DAZSBF010000000
VERSION     DAZSBF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.475"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1052
                     /gene="ospD3"
                     /locus_tag="ACHXWD_004548"
     CDS             <1..1052
                     /gene="ospD3"
                     /locus_tag="ACHXWD_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR7623196.1"
                     /translation="IFVDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTR
                     VVQLSDLSDNTLELLLAAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVE
                     LLTAEGMGGRISGLSQALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVH
                     RQIPGLMFALQNGHADAIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNG
                     QADAILAYGDILNEAKLNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHT
                     ADLTPHQASKLLAAEGPNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDK
                     KNGSDFLEIMKNIKS"
BASE COUNT          417 a          226 c          243 g          334 t
ORIGIN      
        1 ctatatttgt ggacagacat actccaacaa gcatgtcctc cataatcaga tggcctaata
       61 atttacttca ccccaaagtt atttatcacg cgatgcgtat gggattgact gagctaatcc
      121 aaaaagtaac aagagtcgta caactatctg acctttcaga caatacgtta gaattacttt
      181 tggcagccaa aaatgacgat ggtttgtcag gattgctttt agctttacaa aatgggcatt
      241 cagatacaat cttagcatac ggagaactcc tggaaacttc tggacttaac cttgataaaa
      301 cggtagaact actaactgcg gaaggaatgg gaggacgaat atcgggttta tcccaagcac
      361 ttcaaaatgg gcatgcagaa actatcaaaa catacggaag gcttctcaag aagagagcaa
      421 taaatatcga atacaataag ctgaaaaatt tgctgaccgc ttattattat gatgaagtac
      481 acagacagat acccggacta atgtttgctc ttcaaaatgg acatgcagat gctatacgcg
      541 catacggtga gctcattctt agcccccctc tcctcaactc agaggatatt gtaaatttgc
      601 tggcctcaag gagatatgac aatgttcccg gacttctgtt agcattgaat aatggacagg
      661 ctgatgcaat cttagcttat ggtgatatct tgaatgaggc aaaacttaac ttggataaaa
      721 aagcagagct gttagaagcg aaagattcta atggtttatc tggattgttt gtagccttgc
      781 ataatggatg tgtagaaaca attattgctt atgggaaaat acttcacact gcagacctta
      841 ctccacatca ggcatcaaaa ttactggcag cagaaggccc aaatggggta tctggattaa
      901 ttatagcttt tcaaaatagg aattttgagg caataaaaac ttatatggaa ataataaaaa
      961 atgaaaatat tacacctgaa gaaatagcag aacacttgga caaaaaaaat ggaagtgatt
     1021 ttctggaaat tatgaagaat ataaaaagct gaatattatc tcgtggtgat acactgaatt
     1081 tgaggtaacc ggctcattta aaccgtctgg tctgttacct ccggttccac aaaaataatg
     1141 tccatcattt tgaagtggac actatcgtat ggaataccgg acctggatca ctgaagcttt
     1201 acgccttcat ttcgaagaac
//
LOCUS       DAZSBF010000468         1210 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZSBF010000468 DAZSBF010000000
VERSION     DAZSBF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.476"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            54..1210
                     /locus_tag="ACHXWD_004549"
                     /pseudo
     CDS             54..1210
                     /locus_tag="ACHXWD_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT          321 a          305 c          272 g          312 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca
//
LOCUS       DAZSBF010000469         1208 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZSBF010000469 DAZSBF010000000
VERSION     DAZSBF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.477"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..393)
                     /locus_tag="ACHXWD_004550"
     CDS             complement(<1..393)
                     /locus_tag="ACHXWD_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR7623197.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKN"
     gene            complement(410..>1208)
                     /locus_tag="ACHXWD_004551"
     CDS             complement(410..>1208)
                     /locus_tag="ACHXWD_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR7623198.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          319 a          267 c          239 g          383 t
ORIGIN      
        1 attcttatct tttattttat ttactgtttc aatgcaatca gcggcaagtt ggagtggatt
       61 tagatttttc agatatggtc ttatgatctt tcccttactt aataaatagc atgtatagga
      121 ttcggaaagt ccagcatcta ttttgtacct cattatatta gataaggatg tgagaaattc
      181 cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg ccttacaggc
      241 aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca
      301 taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgcact
      361 cgcagaaata gagaaaaaaa tacaattgtt aataactttg ccatattttt tagtccgtaa
      421 ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag
      481 atgaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga ccgaaagcgt
      541 ttaaacaact ttgtgttaaa aatccatcac gattatatgt ttctgtattt gctgacggta
      601 ctaaacgtat accattaggt tttctattgg ttgctccagg accattaaca ttccagctcc
      661 ataccccttg aggtattgga cctctatttt gtaagtgtgt acaaccagga ttatttttac
      721 attgcatatt attaccatta ttaccagagg cgacaggaat agtaagcaca tcataagagt
      781 tatcatcatt ttgcgtatct gaaacgcatg aaagcatact taaagttatg ctgtataaac
      841 aggtcgctaa tcccaacggg tcaatatcag ttatcggatt caacggatac tgataaaaat
      901 tccatccccc cttcagtcca atcggatcct gagtgatata tcgcccctgc agcgggtcat
      961 aatagcggtg gcggttgtaa tacaggccgg actcctcatc atactgctgc cccggcaggc
     1021 ggataagctg ctgcagctga tgcgggttct cttcattcag caggttgccc cactcatcgt
     1081 attctgcgca ccattctgtt gccccttccg tgctgataag caccagcggc aggccgcgat
     1141 ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta caccgggtcc atctggtttt
     1201 gcatctgc
//
LOCUS       DAZSBF010000470         1200 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZSBF010000470 DAZSBF010000000
VERSION     DAZSBF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.478"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            223..1038
                     /gene="yahL"
                     /locus_tag="ACHXWD_004552"
     CDS             223..1038
                     /gene="yahL"
                     /locus_tag="ACHXWD_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR7623199.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          365 a          230 c          234 g          371 t
ORIGIN      
        1 tggtttaaca tattaacttc gctctccact taacttttta gttaaggaga gcgaaataat
       61 atcaaagtag cagtaaaacc tataacgtaa attcaaattg ttaaattaac tccctccagt
      121 acacaatact tctcacgtta gttatgagcg atttctgata gtgcctggtt taatcagcgc
      181 tttattatct gcgacgttta tttttattta agagagatcg cgatgatatc attaaaagct
      241 ccgcacaata atttgatgcc atatacgcaa caaagcatac ttaatacggt taaaaacaat
      301 cagttaccag aggatatcaa aagctcccta gtttcctgtg tcgatatatt caaggttttg
      361 attaaacaat attatgatta tccttatgat tgtcgtgatg atttagtcga tgacgacaaa
      421 ctcattcatc tcatggctgc cgtacgagac tgtgagtggt cagacgataa cgcactcacc
      481 ataaatgtgc agtttaatga tttcccagga ttttatgact ggatggatta ccctgatcac
      541 ccggttaagt ttgtttttcg catacttgaa aatcaaaaag ggacagtttg ggtatatgac
      601 caggatgatg cgtttcttga tattaaagca aatgtgcagg ctggacgctt taccgggctt
      661 aagaaattag tgcagtttat caattcagtg cgaacagatt gcaaatgtat tcttcttgaa
      721 caccatatgc ctttgctgag acttttccct aaagggaaag agtgtatgca tgtggaaaaa
      781 tggcttcgcg aaatgtcgtc tattccggag acagacgcgc ccattaagca ggcgcttgcg
      841 catggattat tattacactt aaaaaatatc taccctgttt ttcctgaatc actggtgatg
      901 ttattgctct ctgttctgga cgtaaaaaca tacagagatg atgcacgatt aaatgaatgg
      961 atatctaata gagtgcagga attaggtgac agatattatc ctgtaaataa gcacgttaaa
     1021 atccgctaca ctctttagtt cagaaagggt tccacctgca agttgttatt ccaccagaga
     1081 gtgaatcgca aagcaggtaa tcatttcatc tgtatatccg tatgccaaat ggcattccac
     1141 cgccttttcg ccgatatttt gttgtgatcg gcgacacttc gcttaaaaaa gcaccagcag
//
LOCUS       DAZSBF010000471         1179 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZSBF010000471 DAZSBF010000000
VERSION     DAZSBF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.479"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="ACHXWD_004553"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="ACHXWD_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR7623200.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="ACHXWD_004554"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="ACHXWD_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          356 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctt
//
LOCUS       DAZSBF010000472         1162 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZSBF010000472 DAZSBF010000000
VERSION     DAZSBF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.480"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHXWD_004555"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHXWD_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR7623201.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHXWD_004556"
     CDS             complement(510..>1162)
                     /locus_tag="ACHXWD_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR7623202.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          276 g          257 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cgggggtcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZSBF010000473         1149 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZSBF010000473 DAZSBF010000000
VERSION     DAZSBF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.481"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            2..>1149
                     /gene="ipaH1.4"
                     /locus_tag="ACHXWD_004557"
     CDS             2..>1149
                     /gene="ipaH1.4"
                     /locus_tag="ACHXWD_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HFR7623203.1"
                     /translation="MQAIGSSIMHQINNIYSLTPFSLPMELTPSCNEFYLKAWSEWEK
                     NGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNLLTHLPD
                     LPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHNNRLHIL
                     PLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVLMNNNLT
                     TLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSATISAPE
                     HSLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQV
                     AAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVHQAS"
BASE COUNT          350 a          273 c          215 g          311 t
ORIGIN      
        1 tatacaggca atcggttcta gtattatgca tcaaataaac aatatatact cgttaactcc
       61 attttcttta cctatggaac tgactccatc ttgtaatgaa ttttatttaa aagcctggag
      121 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctgaa
      181 aatatgttta caaaatcaag aggcagaatt aaatttatct gagttagatt taaaaacatt
      241 accagattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacacatct
      301 ccctgattta ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      361 acctgcctta cccgagacgt tagaagagct taatgcgggt gataacaaga taaaagaatt
      421 accatttctt cctgaaaatc taactcattt acgggttcat aataaccgat tgcatattct
      481 gccactattg ccaccggaac taaaattact ggtagtttct ggaaacagat tagacagcat
      541 tccccccttt ccagataagc ttgaagggct ggctctggct aataatttta tagaacaact
      601 accggaatta ccttttagta tgaacagggc tgtgctaatg aataataatc tgacaacact
      661 tccggaaagt gtcctgagat tagctcagaa tgccttcgta aatgttgcag gtaatccatt
      721 gtctggccat accatgcgta cactacaaca aataaccacc ggaccagatt attctggtcc
      781 tcgaatattt ttctctatgg gaaattctgc cacaatttcc gctccagaac actccctggc
      841 tgatgccgtg acagcatggt tcccggaaaa caaacaatct gatgtatcac agatatggca
      901 tgcttttgaa catgaagagc atgccaacac cttttccgcg ttccttgacc gcctttccga
      961 taccgtctct gcacgcaata cctccggatt ccgtgaacag gtcgctgcat ggctggaaaa
     1021 actcagtgcc tctgcggagc ttcgacagca gtctttcgct gttgctgctg atgccactga
     1081 gagctgtgag gaccgtgtcg cgctcacatg gaacaatctc cggaaaaccc tcctggtcca
     1141 tcaggcatc
//
LOCUS       DAZSBF010000474         1148 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZSBF010000474 DAZSBF010000000
VERSION     DAZSBF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.482"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHXWD_004558"
     CDS             <1..122
                     /locus_tag="ACHXWD_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623204.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHXWD_004559"
     CDS             119..376
                     /locus_tag="ACHXWD_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623205.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHXWD_004560"
     CDS             376..750
                     /locus_tag="ACHXWD_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR7623206.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHXWD_004561"
     CDS             747..>1148
                     /locus_tag="ACHXWD_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR7623207.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZSBF010000475         1147 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZSBF010000475 DAZSBF010000000
VERSION     DAZSBF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.483"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            23..706
                     /gene="tssD"
                     /locus_tag="ACHXWD_004562"
                     /pseudo
     CDS             23..706
                     /gene="tssD"
                     /locus_tag="ACHXWD_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            686..>1147
                     /locus_tag="ACHXWD_004563"
     CDS             686..>1147
                     /locus_tag="ACHXWD_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623208.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          349 a          229 c          256 g          313 t
ORIGIN      
        1 attaacaaaa ggtttttcaa ggatgagtga tattatttac ctgaccataa ccggagaaca
       61 acaaggatgt atttcctctc gttgtggaac gagcgcatca ataggtaatc gctggcagat
      121 tggccatgat gatgaaatct tcgcgttctc gctctctaat agtattacga atacaggcaa
      181 agggtcataa ttgcatggcc ttagtttctg taaattgatt gacaaaagct ccccgttact
      241 tattaacgct attaacaata acgagcagtt gttcatggag tttgacttct atcggatcaa
      301 taggtttggt cgatgggaga aatattacaa cattcaactc cgcggcgcgc tcttatcagc
      361 aatcaatcac ctgtttactg agaacaatct ggatacagaa gccatcactg tcagttatta
      421 atatatactc tccaggcatc tgattgctaa tactgaattt agttattggg catttcccga
      481 taactacaac cgtctgttta taccacggcc aaaaactaaa gctggtaatg gtttcaaaac
      541 acttaatagt aaagcggttg gcagactgct tgctgctgtc ggcatataca atgggaatat
      601 cgaaggattc agggaaacag cagacaagtt ggggggagat gcacctgctg gttatgatca
      661 atttatggat aacaaagggt tactcatcgc tggtgcttca atagttgctg gagcagttct
      721 ggggagaatg agactccctg atttggaatc acttgagcat ttcggagcca gaggagcagt
      781 atcaggaaga ccatttaatc ccgagcttgc cggtggtcca atagaaaacc tgacgacaga
      841 tggcgtaaca atcaacaggg aaggtatcgc tattgttgaa aaacatatag ccagatttgg
      901 tcatgatcct gtaaatgaag tgatgatcaa tagactgaaa gacattgaga aaggaaaaat
      961 ccccccagaa caagtagatc ttaacttcta tactcatgag tgtcgggaat atcagcgtta
     1021 ctgtaatctt ggttgggaaa caggacaacc tgatggtgac gcaggctatg cactctggaa
     1081 tcatactgga tttgccccta tatttccaga catctgttat cacttaaccc attacaagcc
     1141 cgctgcc
//
LOCUS       DAZSBF010000476         1117 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZSBF010000476 DAZSBF010000000
VERSION     DAZSBF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.484"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHXWD_004564"
     CDS             84..1115
                     /locus_tag="ACHXWD_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR7623209.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZSBF010000477         1115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZSBF010000477 DAZSBF010000000
VERSION     DAZSBF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.485"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            11..685
                     /locus_tag="ACHXWD_004565"
     CDS             11..685
                     /locus_tag="ACHXWD_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR7623210.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            701..>1115
                     /locus_tag="ACHXWD_004566"
     CDS             701..>1115
                     /locus_tag="ACHXWD_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR7623211.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          358 a          201 c          252 g          304 t
ORIGIN      
        1 aggcattaaa atgaaaggat tattatcttt actcattttt tctatggtcc ttcctgcaca
       61 tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata aagaagtgat
      121 ggtgcagttg atgaaccagg gaaaccgttc ttcgctgctg caggcgtgga ttgatgatgg
      181 cgatacgtca ttaccaccag aaaaaattca ggttcctttc atgttaacgc caccagtggc
      241 aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga ataaactgcc
      301 cactaataaa gaaagcattt tttatctgaa tgttctggat attccaccaa atagtccaga
      361 gcaagaaggt aagaatgcac tgaagtttgc gatgcaaaac agaattaagt tgttttaccg
      421 accagcgggt attgctccgg taaataaagc gacatttaaa aaattgctgg taaatcgcag
      481 tggcaatggt ttggtgataa aaaatgactc agctaattgg gtgacgattt cggatgtcaa
      541 agctaataat gtcaaagtca attatgaaac tattatgatt gcccccttag aaagtcagag
      601 tgttaatgtc aaaagtaata atgcaaataa ctggcatctg accattatcg atgaccatgg
      661 caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact
      721 tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt
      781 tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga
      841 tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg
      901 cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat caagagaagt
      961 tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat gtttaacatt
     1021 tgagcaactt gttcagggtg ggagctatac ctgggatatc ggggtttttc gtctcgattt
     1081 cagtgtcccg caggcctggg tggaagaact ggaaa
//
LOCUS       DAZSBF010000478         1115 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZSBF010000478 DAZSBF010000000
VERSION     DAZSBF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.486"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            53..232
                     /locus_tag="ACHXWD_004567"
                     /pseudo
     CDS             53..232
                     /locus_tag="ACHXWD_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(249..947)
                     /locus_tag="ACHXWD_004568"
                     /pseudo
     CDS             complement(249..947)
                     /locus_tag="ACHXWD_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1026..1115
                     /locus_tag="ACHXWD_004569"
                     /pseudo
     CDS             1026..1115
                     /locus_tag="ACHXWD_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          302 a          250 c          286 g          277 t
ORIGIN      
        1 cctattatag aatgataaat taaaattact gtaaaaaaac gatacacatt taatacgagg
       61 gaggccggca gaaaaacggc ttgcggtaag gcaggagaaa tgtgtgccgc gaatgaagtc
      121 cttgtatgac tggataaagc tccagaggaa aactttgtca aaccatgctg aaatggtaaa
      181 ggcgttcaac tatatcctga atcactggaa tgcactgaat gagttttgcc gggtagtact
      241 tccaatcatt agaatatgtg cttttcaatg aaggaaccga tgactttttc gtggatttcc
      301 acggaacgcg agaagcagat tgttttacgt gccagccgct tgatgcgggg gcggagggtc
      361 aggttgttac gttcatacgt tgcgtgaaga ttttgccggt caggtgtttc tcctccggca
      421 cttctctggc gtagctgccc cagtcatcgc tggtcaccat gccaccatgc cgatacagaa
      481 cggagtcaac agggctaaca gttcccgaca ggtctcatca ttccgtggcc cgcaagtgta
      541 agccaacaca ccgccagttt tagtgttgta cgcataccag ggccagtgtt gttgcgcctt
      601 gctgccaacg aagcgccact gctcatcaag ttcacagata agggcaacat cagcgtgagc
      661 gactggcgca gatgttatcc gccggggcga gagcttttta atgtccgtat gacggtattg
      721 atgccgattt tcagtgttct ggcggtataa cagccccctg cgccgttatg tgccatttca
      781 acgatctgct ccttaacgcc gggttttctg gcttcataag agtaagtgag ctggaaaaca
      841 cgtttacatg agcgacaacg gaaacgctca tgccgggaac aactacggcc atgtcgataa
      901 acttcatcgg aatgacagcg cggacaatga acagtaacgg tagtcatgga aaacctcaaa
      961 taccagtaga ttaccagaaa cttctatcga ttgaagacat taccttttgc cgcaataggt
     1021 ggggtaacat tggtgaaaac gcattacgcc gggtggcagt tggaaaaaaa ttatcttttt
     1081 tcggctctga tcgtggtgga gaaaatgcgg caata
//
LOCUS       DAZSBF010000479         1110 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZSBF010000479 DAZSBF010000000
VERSION     DAZSBF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.487"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(100..1104)
                     /gene="argF"
                     /locus_tag="ACHXWD_004570"
     CDS             complement(100..1104)
                     /gene="argF"
                     /locus_tag="ACHXWD_004570"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR7623212.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          253 a          292 c          265 g          300 t
ORIGIN      
        1 aataaaaaaa ttggggtgga ataattgaac tatcactgca taaaataatg tgacgccggg
       61 atgtttttgt atttcccggc atctttatag cgatatcaat tatttactga gcgtcgcgac
      121 catcaccgct ttgatggtat gcatgcggtt ttccgcctga tcaaatacaa tgctggcggc
      181 agattcgaag acctcatcag tcacttccat tccgccatgt aggccaaact cttccgccat
      241 ttttttgcca agcgtcgttt ggtcgtcatg aaacgcgggc aggcagtgga ggaatttgac
      301 ctccgggtta ccggtcaact gcatcatctt gctgttcact tgataatcac gcagcaatgc
      361 aatgcgttca gcccatttct cttttgcttc acccatcgac acccatacat cggtatagat
      421 aaagtcagca ccttcaactc ctttagctac atcttccgtc agcgtaatat tcccgccatt
      481 ttgttgtgcc agggcgcggc attccgtaac cagcgcagct tccggccagc atgcctgtgg
      541 cgcgaccaga cgcaaatcca gaccggtaag cgctgcagct tcgagcatcg aattgcccat
      601 gttgttacgt gcgtcacctg catagactag cgtcatttcg ttgaacgttt tgccgggcaa
      661 atgctcctgc atggtgagga gatccgccag cagctgcgtg gggtgaaact cattggtcag
      721 gccgttccat accggcacgc cagcgtattc cgccagtgtt tcgacaatct cctgaccata
      781 gccgcgatac tgaataccgt catacatgcg accaagcacg cgggcagtgt ctttaatcga
      841 ctctttatga ccaatttggc tgccgcttgg gccgagataa gtaacacgag ccccctggtc
      901 atatgcggca acttcgaaag agcatcgggt acgggtcgag tctttttcga agatgagcgc
      961 gatgttttta ccagtgagtt tggcttcttc tttaccgctt ttcttatcgg ctttcagctt
     1021 cgcggctaac tgcagcaggc tgttgagttc agctggcgtg aaatcgagta atttcaggaa
     1081 atgcttatga taaaacccgg acataggtcc
//
LOCUS       DAZSBF010000480         1107 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZSBF010000480 DAZSBF010000000
VERSION     DAZSBF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.488"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..1107
                     /gene="rhsC"
                     /locus_tag="ACHXWD_004571"
                     /pseudo
     CDS             1..1107
                     /gene="rhsC"
                     /locus_tag="ACHXWD_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          276 a          325 c          335 g          171 t
ORIGIN      
        1 cacgggccgg ccggaaatcc gttaccgtta cgacagcgac gggcgggtga cagaacagct
       61 taacccggca ggcttaagct acacgtatca gtatgagaag gaccgcatca ccatcaccga
      121 cagcctgaac cgccgtgaag tgctgcacac gtagggtgaa gccgggctga agcgggtggt
      181 gaaaaaggaa cacgcggacg gcagcgtcac gcagagtcag tttgacgccg tgggcaggct
      241 caggacacag acggatgccg caggccggac aacagaatac agcccggatg tggtgacggg
      301 cctcatcacg cgcatcacca cgccggatgg cagggcatcg gcgttttact ataaccacca
      361 cagccagtta acgtcagcca ccgggcctga cgggctggaa atgcgccgca aatatgatga
      421 atccggccgt ctgattcagg aaaccgcccc tgacggcgat atcacccgct accgttatga
      481 taatccacac agtgacttac cctgcgcaac ggaagatgcc accggcagcc ggaaaaccat
      541 gacatggagc cgttacggtc agttgctgac cgtcactgac tgttccggtt atgtaacccg
      601 ttatgaccat gaccgttttg gtcaggtgac ggcggtgcac cgcgaggaag ggctgagcca
      661 gtaccgcgca tacgacagcc gtggacagtt aattgcggtg aaagacacgc agggccatga
      721 aatgcggtat gaatacaacg ccgccggtga cctgaccgcc gtcattgccc cggacggcag
      781 cagaaacggg acacagtacg atgcgtgggg aaaagccatc tgtaccacgc agggcgggct
      841 gacgcgcagt atggaatacg atgctgccgg acgggtcatc cgtctgacca gtgaaaacgg
      901 cagccacacc accttccgtt acgatgtact cgaccggctg atacaggaaa ccggctttga
      961 cggccgcaca cagcgttatc accacgacct gaccggcaaa cttatccgca gcgaggatga
     1021 ggggctggtc acccactggc actatgacga agcagaccgc ctcacgcacc gcaccgtgaa
     1081 gggtgaaacc gcagagcggt ggcggta
//
LOCUS       DAZSBF010000481         1107 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZSBF010000481 DAZSBF010000000
VERSION     DAZSBF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.489"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHXWD_004572"
     CDS             complement(270..1013)
                     /locus_tag="ACHXWD_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR7623213.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          291 a          274 c          269 g          273 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaa
//
LOCUS       DAZSBF010000482         1102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZSBF010000482 DAZSBF010000000
VERSION     DAZSBF010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.490"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>1102
                     /gene="mxiD"
                     /locus_tag="ACHXWD_004573"
     CDS             <1..>1102
                     /gene="mxiD"
                     /locus_tag="ACHXWD_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFR7623214.1"
                     /translation="KVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPP
                     ALVELVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVV
                     ERLLNNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPET
                     NSILVKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGD
                     SFGASFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRT
                     FYVSLVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENT
                     SVLPEVGRTKISTIARVPQGKSLLIGGYTHETN"
BASE COUNT          371 a          162 c          221 g          348 t
ORIGIN      
        1 aaagttattt tgctggagaa tatatcactt aattatctta ttcagtatct gaaggatgct
       61 aacctttatg atcacagata tcctataaga ggaaatatta gtgataaaac attctatata
      121 tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct tgataagcag
      181 gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa aaatacattt
      241 gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg ggtggctact
      301 gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc ccaaaatgat
      361 ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga cttttcattt
      421 agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata tccagaaacc
      481 aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga tattatcact
      541 cagttagatg tagccaaacg acatatagaa ttatcattat ggataatcga tattgataaa
      601 tctgaattaa ataaccttgg tgttaattgg caaggaacgg cttcatttgg tgattcgttt
      661 ggtgcgtcat ttaatatgag ctcatcagca tcgataagca ctttggacgg caataagttt
      721 attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc aaggccggtg
      781 attttgacac aagagaatat tccggctata tttgataata acaggacttt ttatgtgtca
      841 ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt gataaatgtt
      901 atacctcgct tttcatctcg gggacaaatt gagatgtctt taacgataga ggatggaaca
      961 ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct tccagaagtt
     1021 ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt gttgataggt
     1081 ggttatacgc atgaaacgaa ct
//
LOCUS       DAZSBF010000483         1102 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZSBF010000483 DAZSBF010000000
VERSION     DAZSBF010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.491"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            261..389
                     /locus_tag="ACHXWD_004574"
                     /pseudo
     CDS             261..389
                     /locus_tag="ACHXWD_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            579..983
                     /locus_tag="ACHXWD_004575"
     CDS             579..983
                     /locus_tag="ACHXWD_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623215.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          338 a          240 c          271 g          253 t
ORIGIN      
        1 atcaatgatg tttatgtcag aaagtaataa tcctctccca caaagtgaat taaatgaata
       61 tataatgcgt tcgaagtata tgtattgagc tgtcatactg agaaacaaac cttagggcag
      121 cacaattaaa gccagaaaca actatgcaag aaagttacta aataagtgct cggaacagta
      181 aaacactcag gggggactca gtccggatta tgtaatcaga taaattgcca aacatcacaa
      241 atcaccagcc ggactgagtg atgccgagca ttgcaccaat accccgtgat gaacgatgcc
      301 tgatgcagaa agctccctat aaaacaaaga acaaaaacta cgaccggcga ttcaccgtca
      361 tactgatgct gcatcgtggt gacactgtct gtcactttca tgttgactga acaactctgc
      421 tgcgaagaag gtgtaccgcc ggcgggattt gattgtccgg cgtctagctt ccacttccct
      481 gatggaactg aaagttgtta tggccggtca gcttgccctg tccgcaggac atgaggtgct
      541 gtgacggaca gcaccagtaa cggaaagtga caatgaaaat gaatactgcg ctgatagttg
      601 cactaatgtg tatgtggtat gcggtacccg ctgctgcaaa agagacttta ctggctatgc
      661 cacgtaattc aacagaacat tgctatgcgg agattaatgt gcacggacca tatggggtgt
      721 atttccgagt ggtcccgcat ccccccgggg gaaagagctg ggtcgaatgt aacagtgact
      781 actattactc tgataaacca ccgggagtac agatactcgg aacccgtgca gggtgtcggg
      841 tatacggaat ctgcgggacc acctcgacac tgcatgtggc tggaagaggg gttgtgtgta
      901 ttaaaaatat atgcagtcca cgtgggatga taattcacag gatacggaaa cgtcctgtag
      961 tggctgtaag cgatgagatg tgatcttacc cagcaatagt ggacacgcgg ctaagtgagt
     1021 aaactctcag tcagaggtga ctcacatgac aaaaacagta tcaaccagca aaaaaacacg
     1081 taaacagcat tcgcctgaat tt
//
LOCUS       DAZSBF010000484         1101 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZSBF010000484 DAZSBF010000000
VERSION     DAZSBF010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.492"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1101)
                     /gene="ospC2"
                     /locus_tag="ACHXWD_004576"
     CDS             complement(<1..>1101)
                     /gene="ospC2"
                     /locus_tag="ACHXWD_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFR7623216.1"
                     /translation="AVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSSSGISE
                     KHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSLEHLDK
                     VSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFDKIKSE
                     NIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDM
                     RLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFK
                     KIRLRDISLEDAIKASNYEEINNKVTDKKMAH"
BASE COUNT          343 a          178 c          183 g          397 t
ORIGIN      
        1 tgatgagcca tttttttatc agtcacctta ttgttaattt cttcataatt ggatgcttta
       61 atggcatcct ccaaagatat atctctgagt cttatctttt tgaagttatc tgtgcttagc
      121 attcttggta tatgatactc aagctgaaac acaaagttta taattctatc cagactaaca
      181 ggatcaatgt ttttattata acaaaactct cggaatcttt gatctttact ctttcttata
      241 aaatcaataa gatgcaaccc aattccaagc ctcatatctt tagtattaaa tattggcaca
      301 tcagtttttc cctgattacc atgcacatac ctgctaactt catccacaac ctctttaaag
      361 ttatctaaga aaaatgattg gtgctcatgg taaaaaacag gtggaatagc attatcaaag
      421 tggtcggtta atgtcatata tccatatgga gagtcatgat caatgatata cgcattagca
      481 ccaaaatcta cagtatgatg tgttttattt agtgggagtg tttcttgatg attggaaatt
      541 tcaaccccaa aaaaaacaaa atcgtggtta gctatttctt caatatcctc agagaatgtg
      601 tttttatatg tttgtatatt ctcagactta attttatcaa aggacttaat attaagagta
      661 ttatttgaca ggttagcgtt actttgatgt ctgaagttaa aatcagaatt aataataata
      721 gagagcaagt gacgttcatc cgaagaaaga ggatgagttt tttgtattat ttcagaaaga
      781 tagtgtctgg acaccttatc gaggtgctca agtgaccgaa atgatgaccg tgctccagat
      841 ggcgcttcaa tagaaagatt taatgatttg aaactggtcc cttgagagaa cattttacta
      901 taactttgcg ctgctataga cttccttaaa aagcttttca cagtatcagc acagtgatct
      961 aaatgttttt cacttatgcc agaagaggat gagtttgtta cgcatgatat gtttttttgt
     1021 aatgcttttg tatctttgtt cggatttatt tttccatcaa caagatcaat attgttctga
     1081 acattaatat gatttactgc t
//
LOCUS       DAZSBF010000485         1099 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZSBF010000485 DAZSBF010000000
VERSION     DAZSBF010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.493"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1099)
                     /gene="mxiA"
                     /locus_tag="ACHXWD_004577"
                     /gene_synonym="virH"
     CDS             complement(<1..>1099)
                     /gene="mxiA"
                     /locus_tag="ACHXWD_004577"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFR7623217.1"
                     /translation="ERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSF"
BASE COUNT          398 a          244 c          155 g          302 t
ORIGIN      
        1 aaaactatcg gcacgcacct catttattaa caaaacaata tcatcgacct taagctcatt
       61 gcttgttcta tataaaatag ttggaagcct gacaccataa tcaataaaga actgacttct
      121 tatcctttca ataagacctt ccatatcatt tgcatttatc ttattttcgg caaataataa
      181 aattaaagga acggtttctg aactaatagc atccagattt tctatcattg ccaaagatga
      241 gtcatggcta ttatcaatat caaatgtacc agtatagcca ctagaatcag actcagacag
      301 acttttttct ttttctacga cctttttata atagaataaa gccgtcaaag taactgctat
      361 caggaaaaaa acaaaaaacg gaaaacctgg caacatccct atcgccaagg caagagctga
      421 tgtaacaatg agaacaaaag gattcccaaa tatctgggac attatgttac gtcccatatt
      481 atcgctatca ccattaactc ttgtaacgat aaatcctgca cttatagaaa ttaacagagc
      541 aggaatctga gagaccaatc cgtcaccaat agttagtatg gtataagtag ataatgcacc
      601 agatagggac attccatgtt gactcatacc aacagaaata cctcctatta aattgacaaa
      661 aataataatg atgccagcaa ttgcatcacc tttaatgaac ttcattgctc catcaaatga
      721 accatacaat tgactctcac gttccagaat actgcgtctt tctttagctc ctgcggcatc
      781 aataattcca gcctttaaat ccgcatcaat gctcatttgt ttccccggca taccgtcaag
      841 agaaaagcga gcagctacct ccgcaaccct ttcagatcct tttgttataa caatgaactg
      901 cacaacagtt actattgaaa aaataacaaa acctacagcc agactatcac caatgacaaa
      961 ctggccaaat gtagtaataa ttttacccgc atccgcatca actaaaatta gtcgactagt
     1021 acttattgat aaagcgagac gaaataatgt cgttatcaac aaaaccgaag ggaatgtaga
     1081 aaaactcaat attctttca
//
LOCUS       DAZSBF010000486         1095 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZSBF010000486 DAZSBF010000000
VERSION     DAZSBF010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.494"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHXWD_004578"
     CDS             <1..468
                     /locus_tag="ACHXWD_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR7623218.1"
                     /translation="DVCHYDSETKVLTSHHGIIRNEENECYCLGALQVPFNQMKDPID
                     PEELANIFNKKHKQHMDDRLIHLIRSNAVASTPVYENYFNNTTAFRPKPEDIFKCGQT
                     LKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            730..>1095
                     /locus_tag="ACHXWD_004579"
     CDS             730..>1095
                     /locus_tag="ACHXWD_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623219.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLD"
BASE COUNT          386 a          175 c          206 g          328 t
ORIGIN      
        1 gatgtttgtc attatgactc agaaacgaaa gttttaacca gtcatcacgg aataataaga
       61 aatgaagaaa atgaatgtta ttgtctcggt gctttgcagg ttccatttaa tcagatgaaa
      121 gacccaattg atcctgagga attagcaaat atatttaata aaaaacataa acaacacatg
      181 gatgatcgtc ttatccatct aattagaagc aatgctgtag catccacacc tgtatatgaa
      241 aattatttta ataacactac tgctttcaga ccaaaacctg aagatatctt caaatgtgga
      301 cagacgctca aaaaaactaa acagaaatat ggccattatg gattaggggt agatcagcat
      361 caaaaaattg ataattatac catggggcta aattcctgga agatagcacc taacgaacgt
      421 ggtgataaaa aaggtgtccc agggcttagt tgttttcaac ctcaataaaa cttcatgagt
      481 gctgtggtat ctctttttaa ttttgccagt atttcatagg ttttcttaat cgattgtaga
      541 ggcatgttct gatgacgcct ctacaatctt tagggacttt ctctgctatg agataagaaa
      601 tttcatatta tgctttaagg ctgaatgatt tgtgcaaaat ataattattt tcccatcgaa
      661 catgaggtgt atgtcataag attaaatgtt aactatgtat tcctcgcggt aaataatatg
      721 aggtaaaaga tgaaaattgg aactgtggca ggcactaacg acagtacaac gacaatcaca
      781 acaaatgata tggtgcaaga gcatgttacc aacttcacta aagaactgtt tgggtatatt
      841 gcaaatggta taggtgatga tatatcaagt attgccagga caatgctcgg tgaggttgtg
      901 gaaaaaatag atgattggca aatagaacgg ttccaacaat ctattcaaga tgacaaaatt
      961 tcatttacca tacaaacgaa tcattcagaa aaatatagta tgctatcggg catgcgcgct
     1021 catattctac gtaggaataa ctgttatcag tttattgtga caattaatag taaaaattac
     1081 ggttgccctc ttgat
//
LOCUS       DAZSBF010000487         1087 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZSBF010000487 DAZSBF010000000
VERSION     DAZSBF010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.495"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            20..>1087
                     /locus_tag="ACHXWD_004580"
     CDS             20..>1087
                     /locus_tag="ACHXWD_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFR7623220.1"
                     /translation="MKNFIPFALPEIGEEEIAEVIDSLRSGWITTGPKAKQFEQEFSN
                     YLGANVQSLAVNSATSGLHLALEAVGVKPGDQVIVPSYTFTATAEIVRYLGADPVIVD
                     VDRKTFNISVDAIEKAITNETKAIIPVHFAGLACDMDSILSIAKKYDLKVVEDAAHAF
                     PTTYKGSKIGTLDSDATVFSFYANKTMTTGEGGMVVSKNKDIIERCKVMRLHGISRDA
                     FDRYQSKTPSWFYEVVAPGFKYNMPDICAAIGIHQLRKIDDFQKKRQRMAKIYDDALK
                     ELPLELPEWPTNASDIHAWHLYPIRLKTDSAINRDDFIKKLSDLGIGCSVHFIPLHKQ
                     PVWRDTYNLNASDFPVSEECYL"
BASE COUNT          325 a          187 c          227 g          348 t
ORIGIN      
        1 ttttgaaaag gtattttaaa tgaaaaattt tattcctttt gcgttacctg aaattggcga
       61 agaagaaatt gcagaggtaa ttgactcttt acgttcaggt tggattacga caggtcctaa
      121 ggctaagcaa tttgaacaag aattttctaa ttacctagga gcgaacgttc aatcattagc
      181 tgttaactct gctacgtcgg gcttacattt ggctcttgaa gctgttggcg taaagccggg
      241 agaccaagtt attgtcccat catatacatt cactgctact gccgaaattg tcaggtacct
      301 tggtgctgat cctgtaattg ttgatgtaga tcgtaaaaca tttaatatat cagttgatgc
      361 cattgagaag gctattacta atgaaacaaa ggcgattatt ccagtacact tcgctggatt
      421 agcttgtgac atggattcaa tcttatcaat tgctaaaaaa tatgacctaa aggttgtcga
      481 ggatgccgct catgcatttc ctacaacata taaaggaagt aagataggaa cgcttgattc
      541 agatgctacg gtttttagct tctacgccaa taaaactatg acaaccggtg aaggcggaat
      601 ggttgtttca aaaaataaag atataattga gcgttgtaag gtaatgcgtt tacatggaat
      661 cagtcgtgac gcttttgacc ggtaccagtc taaaactcct tcttggtttt atgaggttgt
      721 agctccaggg tttaaataca atatgcctga tatctgtgcg gcaatcggta ttcatcaact
      781 tagaaagatc gatgattttc agaaaaaacg tcaacgaatg gcaaaaattt acgatgatgc
      841 gttaaaagaa ttgccacttg aattgcctga atggcctact aatgctagtg atattcatgc
      901 ttggcatcta tatcctatcc gcttaaaaac tgattcggct attaatcgcg atgattttat
      961 taagaagtta tcagatcttg gaattggttg ttctgtccat tttataccgt tgcataagca
     1021 accggtttgg cgtgatacat ataatttgaa cgccagtgac tttccagttt ctgaggagtg
     1081 ttattta
//
LOCUS       DAZSBF010000488         1085 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZSBF010000488 DAZSBF010000000
VERSION     DAZSBF010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.496"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHXWD_004581"
     CDS             complement(<1..643)
                     /locus_tag="ACHXWD_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR7623221.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHXWD_004582"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHXWD_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR7623222.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZSBF010000489         1070 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZSBF010000489 DAZSBF010000000
VERSION     DAZSBF010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.497"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            7..510
                     /locus_tag="ACHXWD_004583"
     CDS             7..510
                     /locus_tag="ACHXWD_004583"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR7623223.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(556..972)
                     /gene="rraB"
                     /locus_tag="ACHXWD_004584"
     CDS             complement(556..972)
                     /gene="rraB"
                     /locus_tag="ACHXWD_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR7623224.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
BASE COUNT          249 a          273 c          252 g          296 t
ORIGIN      
        1 taaactatga ataacattgc gccgcaatca cctgtaatgc gtcgcctgac gctgctggat
       61 aatcctgcta tcgcccgcgt cattcgtcag gtatccgccg aatacggtct taccgctgat
      121 aaaggctaca ccgtcgccga tccgaatctt gacgagctgt atcaagtgta tagtcagcct
      181 ggccatgcat attgggtggt tgagtacgag ggtgaagtgg tcggcggcgg tgggattgcg
      241 ccattaaccg ggagtgagtc ggacatttgc gaactgcaaa agatgtattt tcttccggct
      301 attcgcggta aagggctagc gaaaaaactg gccttaatgg cgatggagca ggcacgagag
      361 atgggcttca aacgctgcta tctggaaacg accgcttttt taaaggaagc cattgcgctt
      421 tatgagcatt tgggctttga gcatatcgac tatgcgcttg gctgcacggg ccatgtcgat
      481 tgtgaagtgc ggatgctacg cgaactctaa aaaaatgccc gctagtatgt gctatcgggc
      541 attttaatca attaattagt ggcgaacccc gtcatcgtct tcatcgacaa aatcttcatc
      601 gtcgccatct tcgccgttag gatcttcaaa gtaagtgccc caaccgtcgt actcaacgtc
      661 aaatttctcc gccagcgtca tcagttgttc aacctgggca tcgatcagat cggcattcaa
      721 cgcgcactcg ctgaggatgt cgcagcaaat cacgatatca ccatcttcaa cttccagctc
      781 ttctggatcg gtcacttcgt aaccgagttt aaacgcttca actgccgctt tttccagagt
      841 ttccagatcg tctgcggaaa gatggtgttc gatggtgtac aacgcgtccg gatcgctgcc
      901 atcttccagt aattcttcaa taatcaaacg tgtttcttca cgctgttctt ccagttgttc
      961 cgggtttgcc atggctcatt cctcttaatg tcctgccgat actcttattg tcacacacca
     1021 ctgacattgc ctccaccttt gtgataaaga tttatgcttt agacttgcaa
//
LOCUS       DAZSBF010000490         1061 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZSBF010000490 DAZSBF010000000
VERSION     DAZSBF010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.498"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..511)
                     /locus_tag="ACHXWD_004585"
     CDS             complement(<1..511)
                     /locus_tag="ACHXWD_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7623225.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIF"
BASE COUNT          345 a          206 c          157 g          353 t
ORIGIN      
        1 caaaaatcac attcgcaatt ttactaccaa tggtatgcct gtataataat gggtgcgatt
       61 caatcgtaaa atgcttgtct aattgatctt gtaatttatt ctcatagtcc agagggaaaa
      121 gaaactgttg tctttcaagt ttcccattcg atacattacg tattccaata gagattcttt
      181 cattacccgc ctttatttca acttcgttat cttctgattt acccagatat tttttaacat
      241 cagtctcgaa agcattacta aaaaaagtat taaattggta attcgctgat ggcatatttt
      301 ccgcgagcaa cacatcattt tttatgagat cactgtcagc atttattgct aatatcagat
      361 gcaagtagat ggtatcccat gtagacaact cacccttacg actggaattt aatatcgcga
      421 aaaattccct cagtgaatcc aggaaattat cactttgatt tagttgtgtg cctggaaatg
      481 atgatgcttg atcagatata ttgtttatca tattaaaaat cacccaagaa aaacgataat
      541 ccaccgaact cctcgtgctt tataattata aaagcacaac gccttttata ttcatattaa
      601 ttaacgagat actgaaacaa cttaatttat tcttcgctaa aattcattag cttaaattct
      661 ttagccacaa ttgtagcata aataatatta tcgagagtca aagctgggac tatcaaattt
      721 cgctcatttt caaaaataca acgtaatttt ggcaatcatc ttaatcgcag gatctcttat
      781 cgcatgtgaa aaattttgta atgattagac aataaccgcg ttcatttatc acgcgttata
      841 aatgtgaaac tggcttcttg cagatgctct taacctctac aggaggattt ataccaccat
      901 ttcagaacta tcatatttac cttaaatgat catttcagcc atataacacg ctcaacctca
      961 caaatggttt acactgtcta ttgtttgggt aatttaagta agtcagctac gagcaacctt
     1021 gagtggatag ccagcaatgg actcaaaccc aatcaggtgt t
//
LOCUS       DAZSBF010000491         1059 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZSBF010000491 DAZSBF010000000
VERSION     DAZSBF010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.499"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1059)
                     /locus_tag="ACHXWD_004586"
     CDS             complement(<1..>1059)
                     /locus_tag="ACHXWD_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR7623226.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     RVPGADEVLPPEPPAYRVLTGVVDGFGRTLAFHRAAEGDVAGAVTGVMDGAGRRFHLV
                     LTTQAQRAEEARKPHTASLSSPDSPCPLSAPSFPDTLPAGTEYGADNGIRLEAVWLTH
                     DPAYPDEQPTAPLARYTYTAGGELRAVYDRSGMQVRGFTYDAEHAGRMVAHHYAGRPE
                     SCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKE
                     HADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQR
                     QVTSVTYPDGLRSSREYDE"
BASE COUNT          164 a          409 c          294 g          192 t
ORIGIN      
        1 ctcatcatac tcccggctgc tgcgaagccc gtccgggtac gtcaccgacg tcacctgccg
       61 ctggctgtta tagccatacc gtaccgtcct gccgtccggc agtatcactg aagtcagctt
      121 tccagacgcc atatgcagtc ggtactccgt ccgccgtccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cagctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgcacctg
      481 catcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaagga
      661 aggagctgag agagggcagg gactgtcagg ggaagataac gaagcggtgt gcggtttacg
      721 ggcctcttcc gcccgctgcg cctgtgtggt cagcaccagg tgaaaacggc ggcccgcgcc
      781 gtccatcacc cccgtcaccg cgcccgccac gtcaccctcc gctgcccggt gaaaggccag
      841 cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg gctccggcgg
      901 cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc acggcccctg
      961 aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg gcagcacctg
     1021 ccagagcgcg cttagcggct gcgaactgtg ctgcgccgc
//
LOCUS       DAZSBF010000492         1046 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZSBF010000492 DAZSBF010000000
VERSION     DAZSBF010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.500"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHXWD_004587"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHXWD_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHXWD_004588"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHXWD_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHXWD_004589"
     CDS             728..985
                     /locus_tag="ACHXWD_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR7623227.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          179 c          198 g          312 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccct
//
LOCUS       DAZSBF010000493         1046 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZSBF010000493 DAZSBF010000000
VERSION     DAZSBF010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.501"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..>1046
                     /locus_tag="ACHXWD_004590"
     CDS             1..>1046
                     /locus_tag="ACHXWD_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7623228.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSILPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          321 a          242 c          175 g          308 t
ORIGIN      
        1 atgcgagaaa ttaatatgct cagaaatatt tcatcctgtt tatttccaca tatcagcaca
       61 attacatccc ccaaccatta tttgtccgaa tgggatgatt gggagaaaca ggggttaccg
      121 gaagaacagc gtactgaggc ggtaagaaga cttcgtgcat gtcttacctc taaggggcat
      181 aaactggacc tgcgagcctt ggcgctttcc tcgttacctg tactccctgc ttgcattaaa
      241 aagcttgatg tgagctgtaa taaattaacc atccttactg atttacctga aaatattaaa
      301 gaacttattg caagagataa tttcttaaca catatatctg cattaccaca ttatctaata
      361 actttggatg tgtccgaaaa tcaattagag aatctgccgt tattaccaga caccatcaaa
      421 tcactaagcg cagagtataa taggttatcc atactgcctt cattaccctt gaatttaaaa
      481 aaacttgagg ttaggaacaa cgaactgcaa actcttccat ctctgccttc taatcttaag
      541 atacttaagg ttgcgcacaa ccatcttact gaactgcccc ctttacctag gagactgcaa
      601 cttctttttg catatagcaa tagattaagc aacttgccaa acatccaaga aaatattatc
      661 atgagaagat ttttttattt tgaaaacaac caaataacta caatcccgac aaatcttttt
      721 cgtttagatc ctcatataac tattgagatt gcaaataacc ccttatcaga tcaaactctg
      781 ctattcttaa tacagcaaac ttcggttcca aattttaacg ggcctcagtt tcgtatttcc
      841 ctgtcagacc aaaacagact gtttttacgc cagatgttgc cgcaaaattt acattcgcgc
      901 catatcagag tcatcactga aggggggcag aactttcaga tcccccctct tcccgaaact
      961 gtggcagcct ggtttcctga agcagatcgt cgggaggttt ctacacaatg gacttctttt
     1021 tccaccgagg agaattcccg ggcatt
//
LOCUS       DAZSBF010000494         1040 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZSBF010000494 DAZSBF010000000
VERSION     DAZSBF010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.502"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(91..1040)
                     /locus_tag="ACHXWD_004591"
                     /pseudo
     CDS             complement(91..1040)
                     /locus_tag="ACHXWD_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          223 a          254 c          209 g          354 t
ORIGIN      
        1 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
       61 ggttgtctgg agattcaggg ggccagtcta ctgtgtttct gttagccatt gtccggcggc
      121 tttttgctgg gtaatgcttg cctgcacctc tctggctgat ctttgccaat atttagaagt
      181 taagaggagg tcgtccgcta aatcttccag acgactaccg atctgtgcgt cggtatagcc
      241 ctttttcacc attccgttga cagcttttga aattaaacca gcagagttat tggcaaagtc
      301 tttcaaatcc tttttagtta agaatccttc attcattaaa gtgttaatac cagaatcacc
      361 gcccttccat atgcctttcc ctgttttctt atcaactaca tattcagcct cgtcatagct
      421 tttggtagcg cgttccttta catctttacc gatggcatct agtatttttc tgcgtcccgg
      481 atcaccctgg aggctatcga tcccgttgtc atacgcccaa cgctcaagat tattaagtaa
      541 gttgtaatca actccaccaa gccttgcacg ttggattagc tccccttctg tttcagcatt
      601 agcaaagctg ttagccataa attcagaggt tttactaaca gccatcacgc cagcagcagc
      661 cgtagcgcct ccaaataaca gagcgcccat cgtggcggaa ttccctgatc ctcccttaac
      721 gggggcaaat tgagcttttc tgttgccatt aatcttacgt tgttgctgtt gtaggcgttt
      781 catctgacta ttcatacgcg ccaggctgat agccccttgc tcatattgtt ttgctatctc
      841 tctagcctgt gcaatagctt tgtattgttc cgctacagag agacggtgca ttgcactgat
      901 agagcttgat acatcaagga gttttaattc tgctttctct ttacgtgctg ctatttgttg
      961 ctgttgcctt gcctgtgctc ttgctgcggc agcttgtgct ctttgattct gcttttctgt
     1021 tttagctttc tgttgagcaa
//
LOCUS       DAZSBF010000495         1026 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZSBF010000495 DAZSBF010000000
VERSION     DAZSBF010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.503"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>1026)
                     /locus_tag="ACHXWD_004592"
     CDS             complement(<1..>1026)
                     /locus_tag="ACHXWD_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR7623229.1"
                     /translation="AAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPE
                     PPAYRVLTVVVDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASR
                     KQRASSLSSPASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTA
                     PLARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRV
                     TEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEY
                     DEAGRLKAQTDAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGL
                     RSSRKYDR"
BASE COUNT          172 a          381 c          283 g          190 t
ORIGIN      
        1 acggtcatat ttccggctgc tgcgtaaacc atccggatat gtcactgacg tcagttgcaa
       61 ctggttgttg taaccatacc gtaccgtcct gccgtccggc agtaccactg aagtcagctt
      121 tccagacgcc atatgcagcc ggtactccgt ccgccgcccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cggctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgaacctg
      481 cgtcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaaaac
      661 ctgagaagag gaaacagaac ggggactggc cggggaagat aaggaagagg cgcgctgttt
      721 acgggacgct tccgcccgct gcgcctgcgt gctcagcgcc atgtgaaaac ggcggcccgc
      781 gccgtccgtc acccccgtca ccgcgcccgc cacgtcaccc tccgccgccc ggtggaaagt
      841 cagtgagcgc ccgaagccat ccaccacgac cgtcagtacc cggtatgcgg gcggctccgg
      901 ccagctgagt atccaccacg gcccctgaag gctgtttgtc gccaggtaca catgcggact
      961 caggcgaaca tcttccggca gcacctgcca gagcgcgctt agcggctgcg aactgtgctg
     1021 cgccgc
//
LOCUS       DAZSBF010000496         1023 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZSBF010000496 DAZSBF010000000
VERSION     DAZSBF010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.504"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHXWD_004593"
     CDS             complement(77..865)
                     /locus_tag="ACHXWD_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR7623230.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHXWD_004594"
     CDS             complement(905..>1023)
                     /locus_tag="ACHXWD_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR7623231.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZSBF010000497         1018 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZSBF010000497 DAZSBF010000000
VERSION     DAZSBF010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.505"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            122..541
                     /locus_tag="ACHXWD_004595"
     CDS             122..541
                     /locus_tag="ACHXWD_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR7623232.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            541..>1018
                     /gene="umuC"
                     /locus_tag="ACHXWD_004596"
     CDS             541..>1018
                     /gene="umuC"
                     /locus_tag="ACHXWD_004596"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR7623233.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          256 a          216 c          266 g          280 t
ORIGIN      
        1 taagagaacg aaaaatcagc agtctatgca gcgacaaata ttgatagcct gaatcagtat
       61 tgatctgctg gcaagaacag attactgtat ataaaaacag tataacttca ggcagattat
      121 tatgttgttt atcaagcctg cggatctccg cgaaattgtg acttttccgc tatttagcga
      181 tcttgttcag tgtggctttc cttcaccggc agcagattac gtcgaacagc gcatcgatct
      241 gaatcaactg ttgatccagc atcccagcgc gacttacttc gtcaaagcaa gtggtgattc
      301 tatgattgat ggtggaatta gtgacggtga tttactgatt gtcgatagcg ctattaccgc
      361 cagccatggt gatattgtca tcgctgctgt tgacggcgag tttacggtga aaaaattgca
      421 actacgcccg acggtacagc ttattcccat gaacagtgcg tactcgccca ttaccatcag
      481 tagcgaagat acgctggatg tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg
      541 atgtttgctc tctgtgatgt aaacgcgttt tatgccagct gtgagacggt gtttcgccct
      601 gatttatggg gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt tatcgcccga
      661 aacgctgagg caaaggcgct gggtgttaaa atgggcgatc cctggttcaa acaaaaagat
      721 ctgtttcgtc gctgtggtgt ggtttgcttt agcagcaatt atgagcttta cgcagacatg
      781 agcaatcggg tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt
      841 gatgaggcat tctgcgatct gacaggtgtg cgtaattgtc gcgatctgac tgattttggc
      901 agagaaattc gcgcaacggt gctacaacgt acccatctta ctgttggtat ggggatcgcc
      961 cagaccaaaa cgctggctaa gcttgccaat catgcggcaa aaaaatggca gcggcaga
//
LOCUS       DAZSBF010000498         1007 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZSBF010000498 DAZSBF010000000
VERSION     DAZSBF010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.506"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHXWD_004597"
     CDS             complement(<1..906)
                     /locus_tag="ACHXWD_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR7623234.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          284 a          235 c          203 g          285 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggcta
//
LOCUS       DAZSBF010000499         1007 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZSBF010000499 DAZSBF010000000
VERSION     DAZSBF010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.507"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            25..>1007
                     /gene="gspA"
                     /locus_tag="ACHXWD_004598"
     CDS             25..>1007
                     /gene="gspA"
                     /locus_tag="ACHXWD_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR7623235.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          257 a          231 c          269 g          250 t
ORIGIN      
        1 tcaataatta aaacaggaag ttctatgtct acgagaagag aagttattct ttcctggttg
       61 cgtgagaaac gtcagacctg gcgtctgtgc tatttgttgg gtgaggctgg aagtggaaaa
      121 acctggctgg cgcagcaact gcaaaaagat aaacatcgcc gtgtgattac tttaagcctc
      181 gttgtttcct ggcaaggtaa ggccgcatgg atcgttaccg acgataacgc ggctgaacag
      241 ggctgccgtg acagcgcctg gacgcgagat gagatggcgg ggcaattact gcatgcgctt
      301 catcgaactg atagtcgctg ccctcttatt attattgaaa atgctcacct gaatcaccgc
      361 cgcatacttg atgatctcca acgcgcgata tctcttattc ctgacggtca atttttgctg
      421 atcggcagac ccgatcgaaa agtcgaacgt gattttaaaa aacaggacat tgaacttgtc
      481 tcgacaggac gtctgacgga gcacgaactt aaggcaagca tccttgaagg tcagaatatt
      541 gaccaacccg atctcctttt aacctccaga gttctgaaac ggatagcttt attatgtcgg
      601 ggcgatcgca gaaagctggc gcttgccggc gaaacaattc gcttattaca gcaagccgag
      661 cagaccagcg ttttcactgc aaaacaatgg cgaatgattt accgcatatt gggtgataac
      721 cgcccccgga aaatgcagct tgccgtggta atgagtggaa ccattatcgc tattacctgt
      781 ggttggctac tgctgtcctc tttcacagct ccgttccccg ttcctgcgtg gctggtaccc
      841 gtaacgccag tagttaaaca agatatgact aaggatattg ctcatgtggt gatgcgcgat
      901 agtgaggcgc taagcgtgtt atacggcgta tgggggtacg aggtcccagc tgattcggcc
      961 tggtgcgatc aggcagtaag agcggggctg gcatgtaaaa gtgggaa
//
LOCUS       DAZSBF010000500         1000 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZSBF010000500 DAZSBF010000000
VERSION     DAZSBF010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.508"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>1000
                     /locus_tag="ACHXWD_004599"
     CDS             <1..>1000
                     /locus_tag="ACHXWD_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HFR7623236.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYW"
BASE COUNT          293 a          220 c          253 g          234 t
ORIGIN      
        1 agtggtaacc tgatcgaagg tctcactgtg tactttgcct taaaaagcgg ctctgccaca
       61 ttaacgtcat taacagcggt gaccgatcaa aacggaatcg cgacaacaag cgtgaaagga
      121 gcgatgacag gtagcgtcac ggtaagcgca gtcacgaccg ctggtggaat gcaaacagta
      181 gatataacgc tggtggcagg cccagcagac gcctcaaagt cagtccttaa gaacaatcgg
      241 tcatcattga aaggagactt taccgatagt gctgagctac atcttgttct gcacgatata
      301 tcaggcaatc cgatcaaagt ttctgaaggg ctggaatttg tacagtcagg taccaacgtg
      361 ccctatgtgc aagttagtgc aattgactac agtaaaaatt tctcaggcga gtacaaagcc
      421 actgttacag gaggcggaga gggtatcaca acgctgatcc ctgtattgaa tggtgttcat
      481 caagctggtc tgagtaccac aatacaattc actcgcgcag aagacaaaat aatgagcggt
      541 acagtatcag tcaatggtac tgacctaccg acaactacat tcccttcgca ggggttcacc
      601 ggggcgtatt atcagttgaa taatgacaac tttgccccag gaaaaacggc ggctgattat
      661 gagttttcaa gctctgcctc ctgggtcgat gttgatgcta ccggtaaagt gacatttaaa
      721 aatgtcggca gcaattcgga aaggattacg gcgacaccaa aatcaggagg ccctagctat
      781 gtatacgaaa tccgtgtgaa gagttggtgg gtgaacgccg gcgaggcttt catgatatac
      841 agccttgctg aaaatttttg cagcagcaat ggctacacgc tccccagagc aaactattta
      901 aaccacagta gttcccgagg catcgggtca ctgtacagtg aatggggaga tatggggcat
      961 tacacgactg aagctggttt tcaatcaaat atgtattggt
//
LOCUS       DAZSBF010000501          996 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZSBF010000501 DAZSBF010000000
VERSION     DAZSBF010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.509"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHXWD_004600"
     CDS             complement(<1..228)
                     /locus_tag="ACHXWD_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR7623237.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHXWD_004601"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHXWD_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          326 a          167 c          189 g          314 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataa
//
LOCUS       DAZSBF010000502          994 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZSBF010000502 DAZSBF010000000
VERSION     DAZSBF010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.510"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHXWD_004602"
     CDS             <1..153
                     /locus_tag="ACHXWD_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623238.1"
                     /translation="PREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSL
                     VSYLNY"
     gene            172..>994
                     /gene="mxiC"
                     /locus_tag="ACHXWD_004603"
     CDS             172..>994
                     /gene="mxiC"
                     /locus_tag="ACHXWD_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFR7623239.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLS"
BASE COUNT          360 a          134 c          199 g          301 t
ORIGIN      
        1 ccccgtgaga taaaggagtc aagttattat aatactgcag aatataaaag cctcatatct
       61 gaaagagaaa tacaaaagac tacacaaata atcccatctg aaactacttt gctggaagac
      121 gaaaaatcat tggtttcata cttaaattac taactataaa gtaggtgatg tatgcttgat
      181 gttaaaaata caggagtttt tagctctgca ttcattgata agttgaatgc aatgacaaat
      241 tcagatgatg gagatgagac tgctgatgca gagcttgatt ctggcttggc taatagcaag
      301 tatattgact catctgatga gatggcttcc gctctttcgt catttataaa cagaagagac
      361 cttgagaaac tgaaaggaac aaatagtgat agtcaggaac gtattttaga tggggaagaa
      421 gatgaaatta atcacaagat ttttgattta aagagaacgt taaaagataa ccttcctcta
      481 gatcgggatt ttatagacag actaaagaga tattttaaag atccaagtga tcaagtctta
      541 gcattaaggg aacttttgaa tgaaaaagat cttactgctg aacaagtcga atcattaact
      601 aaaattatta atgagataat atcaggtagt gaaaaaagtg ttaatgctgg aataaattca
      661 gctatacagg ctaaattatt tggcaacaaa atgaaacttg aaccacagct tttgcgtgca
      721 tgttatcgtg gttttatcat ggggaacata tcaacaacag atcagtatat agaatggctt
      781 ggtaattttg gttttaatca cagacataca attgtgaatt ttgtagagca gtcactgatt
      841 gtagacatgg attctgagaa accgagctgt aatgcttatg agtttggttt tgtgttatct
      901 aaattaattg caattaagat gattagaact tcagacgtaa tttttatgaa gaaactggaa
      961 tcctcaagct tgctaaaaga tggcagttta agtg
//
LOCUS       DAZSBF010000503          981 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZSBF010000503 DAZSBF010000000
VERSION     DAZSBF010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.511"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            43..828
                     /locus_tag="ACHXWD_004604"
     CDS             43..828
                     /locus_tag="ACHXWD_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623240.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          325 a          177 c          216 g          263 t
ORIGIN      
        1 taaacattac catgattatg acaggcgagg gagtgtccga ttatgaattt aagaagggtt
       61 tgttgtatat gtgtattatt ctcgctgctt gcaggatgtg tctctgaatc ttctattgat
      121 gaaaagaaga aaaaagctca agtcacacaa agtaatatta ataaaaacac tccccagcaa
      181 ctgacagaca aagatttatt cggtaatgaa accactctgg ccgtatccga agaggatatt
      241 caagctgcgc ttgatggaga tgagttccgc gttcccctta attctcctgt aatacttgtt
      301 caatccggca accgcgcacc ggaaaccatt atgcaggagg agatgcgaaa atattatact
      361 gtttccacat tctctggtat cccggacagg caaaagcctc tgacttgtaa caaaaacaaa
      421 gataaaaatg agaacgagga tgttgccagt gctgagaata tgaactggat gcaggcactg
      481 cgttttgtgg ctgcaaaagg gcatcagaaa gcgattattg ttttccagga tacgttgcag
      541 acaggaaaat atgactctgg attgaaatca atagtttggt cagattataa aaatcaaaaa
      601 ttgactgaca cgatgtcact gcgctatctg gtacgattca cgctggtgga tgtagctaca
      661 ggtgagtggg ctgcctggtc gccggtgaat tatgaatata aagtgcttcc accattgccc
      721 gacaagaatg aagccagtac gactgatatg acagagcagc aaatcatgca acttaagcaa
      781 aagacctata aagcgatggt aaaagatttg gttaaccgct atcaataata aattatatct
      841 gccgccagga atttctggcg gaaataatac aaaattcttt gcataagaaa tttttcttga
      901 caaagacagt gcaagaaagg acatggaatc cggagttaaa aactgtttgt tttagaatgt
      961 ttaattattt aactgctgaa t
//
LOCUS       DAZSBF010000504          977 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZSBF010000504 DAZSBF010000000
VERSION     DAZSBF010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.512"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            15..>977
                     /gene="istA"
                     /locus_tag="ACHXWD_004605"
     CDS             15..>977
                     /gene="istA"
                     /locus_tag="ACHXWD_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFR7623241.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFD"
BASE COUNT          233 a          258 c          260 g          226 t
ORIGIN      
        1 attcaggaga gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc
       61 agggaatgag tagccgggcg attgccagag aactggggct ctcccgcaat accgttaaac
      121 gttatttgca ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac
      181 tcctggatga ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa
      241 tcccggcaac ggtaatcgct cgcgagatca gagaccaggg atatcgtggc ggaatgacca
      301 ttctcagggc attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt
      361 tcgaaactga acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct
      421 caccgcttca cgtgttcgtt gctgttcccg gatacagccg aatgttgtac atcgaattca
      481 ctgacaatat gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg
      541 gtggtgtgcc gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg
      601 catatcagac cggtcagcac cggttccatc cttcgctgtg gcagttcggc aaggagatgg
      661 gcttctctcc ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga
      721 tggtgcagta cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg
      781 ggagcactgt cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg
      841 ctaaccaacg aaagcatgaa acaatccagg cccgtccctg tgatcgctgg ctcgaagagc
      901 agcagtccat gctggcactg cctccggaga aaaaagagta tgacgtgcat cctggtgaaa
      961 atctggtgag cttcgac
//
LOCUS       DAZSBF010000505          976 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZSBF010000505 DAZSBF010000000
VERSION     DAZSBF010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.513"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHXWD_004606"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="ACHXWD_004606"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR7623242.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          266 a          236 c          216 g          258 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccaa tagttcatcc agtgcttcaa tatcgcgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggtagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg
      601 cgactaatga cagccaacaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaaaa tgcgaaacat atcgttaata
      961 attaaaggga gtaacg
//
LOCUS       DAZSBF010000506          973 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZSBF010000506 DAZSBF010000000
VERSION     DAZSBF010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.514"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>973
                     /gene="ipaA"
                     /locus_tag="ACHXWD_004607"
     CDS             <1..>973
                     /gene="ipaA"
                     /locus_tag="ACHXWD_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFR7623243.1"
                     /translation="DIKDSHPIQDGLPTPPEDMPDGGPTPGANEKTSQPVIHYHINND
                     NRTYDNRVFDNRVYDNSYHENPENDAQSPTSQTNDLLSRNGNSLLNPQRALVQKVTSV
                     LPHSISDTVQTFANNSALEKVFNHTPDNSDGIGSDLLTTSSQERSANNSLSRGHRPLN
                     IQNSSTTPPLHPEGVTSSNDNSSDTTKSSASLSHRVASQINKFNSNTDSKVLQTDFFS
                     RNGDTYLTRETIFEASKKVTNSLSNLISLIGTKSGTQERELQEKSKDITKSTTEHRIN
                     NKLKVTDANTINYVTETNADTIDKNHAIYEKAKEVSSALSKVLSKIDD"
BASE COUNT          382 a          220 c          148 g          223 t
ORIGIN      
        1 gatataaaag attcgcatcc tatccaagat ggcctcccta cacctcccga agatatgcca
       61 gatggcggac caacccccgg agccaatgag aaaacatccc aacctgtaat tcactatcat
      121 ataaataatg ataatagaac ttacgataat agagtttttg acaacagagt atatgacaat
      181 agctatcacg agaacccaga aaatgatgca cagtctccta cttctcagac aaacgatcta
      241 ttatcccgta acggaaactc attactaaat ccacaaagag cactagttca aaaagtaact
      301 tccgttctac cacactctat atcagatact gtccagacat ttgcaaataa ttcagcttta
      361 gaaaaggttt tcaaccatac tccagataat tcggatggaa taggttcaga cctgttaact
      421 acgagtagtc aagaaagatc tgcaaataac tctctttctc ggggacacag gcctctgaac
      481 atacagaact cttcaaccac cccccctctc cacccggaag gagtgacaag cagtaatgat
      541 aactcatcag atacaactaa aagtagcgct tctctttctc atagagtagc ttcgcaaatc
      601 aataaattca actcaaacac tgattcaaaa gtacttcaga ctgatttttt ctcaagaaat
      661 ggagacacat atttaacacg ggaaacgata tttgaagcgt caaaaaaagt aacaaactcc
      721 ctaagtaatc ttatatctct cattggaact aaatcaggaa cacaagaacg agagttacag
      781 gaaaaatcaa aggacattac aaaatccaca acagaacaca gaataaacaa caaattaaaa
      841 gttacagatg caaatacaat aaactacgta acagaaacca acgcagatac aattgataaa
      901 aatcatgcga tctatgaaaa ggcaaaagaa gtatctagcg ccctcagcaa ggtattgtca
      961 aaaattgacg ata
//
LOCUS       DAZSBF010000507          968 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZSBF010000507 DAZSBF010000000
VERSION     DAZSBF010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.515"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            420..844
                     /gene="yqeK"
                     /locus_tag="ACHXWD_004608"
                     /pseudo
     CDS             420..844
                     /gene="yqeK"
                     /locus_tag="ACHXWD_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            880..960
                     /locus_tag="ACHXWD_004609"
     CDS             880..960
                     /locus_tag="ACHXWD_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623244.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          322 a          145 c          165 g          336 t
ORIGIN      
        1 tctcatttga ttagagcaaa atccgaattc gcagcttctg ataatgtcaa atatcacctt
       61 ttctcatgct ttattctatc aaaaatcgct catttttact ttttttacca taatgttact
      121 cagtagcatc ggcaatttac ataacttaca cgttgttttt tatatctcga aaaaacagat
      181 agatagtgtg cgcactcgaa atgatattgg taacataaat agctttattc gtgataaaca
      241 tctcaaaaga tttcctttat tatattcgaa attcatttga tcaaggttat caccttatgg
      301 agacgttacg tagaatgact atatttgtta tcggcacatt aggggtatta attttagtat
      361 tgatggaagg tgattattga acagtaacat cgagtcacgg taaaaatgtg aggtatacaa
      421 tggacattga gttttcgcag attcatgaaa tggtctatat gcatgatatt gtaaattcgg
      481 actcgaaaaa gaaaccaagg attccgttaa aaaaattcct taatgctgag aatgttctca
      541 cgcaaacaac atcatggaca ttaaattcaa gatatgtgaa tgttaattct gtcaataaag
      601 tcaacgtaaa gagtaaagtt aaaaacagtt atatctcacg atctgtcaat gatgagttta
      661 gtttaacaga tgatgaaatc aattctttta aagaaacgtt agtcctttcc tctattgatt
      721 cctgagtaaa ctggttctta ataatcctct ttctgtattg tttacatcga ctgtacgaag
      781 gaataataat cgagctaaga tgaatgtcga gttcgactca tggatttgca ccagatgttg
      841 ttgatacatg atattttaca ttgcaaggag atgtctaaaa tgaaagatgt tgatcaaaac
      901 tttgatgctt tagactgcca tatactgcga gaatatttaa ttttattgtt ttatgattaa
      961 cgaggttc
//
LOCUS       DAZSBF010000508          963 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZSBF010000508 DAZSBF010000000
VERSION     DAZSBF010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.516"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            19..963
                     /gene="yfdF"
                     /locus_tag="ACHXWD_004610"
                     /pseudo
     CDS             19..963
                     /gene="yfdF"
                     /locus_tag="ACHXWD_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          370 a          170 c          170 g          253 t
ORIGIN      
        1 tcaaaatgga ataaaatcat gctaccatct atttcaatca acaataccag cgcaacttac
       61 ccagaatcca ttaatgaaaa taacaatgat gaagttaatg gattagtaca agagctcaaa
      121 aaccttttta atggtaagga aggaataagc acctgtatta aacatctact tgagcttata
      181 aaaaacgcca tacgagtaaa cgacgatcct taagatttaa tattaataat tcctcagtta
      241 cttatattga tattggctcc aatgatacag accatattac tattggtatc gacaaccaag
      301 aaccaataga attacctgcg aactataaag acaaagaact cgtccgtact atcattaatg
      361 acaacatagt tgagaagact catgatatca ataacaagga aatgatcttc agcgcattaa
      421 aagaaatata tgatggagat cctggtttta tcttcgataa gatatcacac aaactcagac
      481 atacggtaac agaatttgat gagagcggga atagcgaacc aacggactta tttacctggt
      541 acggtaaaga taaaaaaggc aactctctcg ctattgtaat taaaaataaa aacggaaatg
      601 attacttatc tctcggttac tacgatcagg acgactacca cattcaaaga ggaattcgta
      661 ttaatggtga tagtctcacc caatattgta gtgaaaaagc caggaatgct tcagcaatgc
      721 ttcagcgtgg tttgaaagca gtaaagctat catggcagaa tcatttgcaa caggttccga
      781 tcatcaggtt gtaaacgagc tcaacgggga aagactgaga gaacctaacg aagtttttaa
      841 acgtttaggt cgagcaataa gatataattt tcaagtggac gatgcaaaat ttagacgcga
      901 caatgtaaaa gaaataattt ctaatttatt cgctaacaaa gttgacgttg accatcctga
      961 aaa
//
LOCUS       DAZSBF010000509          962 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZSBF010000509 DAZSBF010000000
VERSION     DAZSBF010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.517"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..847
                     /gene="yeeO"
                     /locus_tag="ACHXWD_004611"
     CDS             <1..847
                     /gene="yeeO"
                     /locus_tag="ACHXWD_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR7623245.1"
                     /translation="LFSWPGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLK
                     SYFKPLNFSIIWEVMGIGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAA
                     LINLPGSALGSASTIITGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVM
                     ASFYTQDPQVKHVVVILIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGC
                     RVVVGYVLGIMLGWGVVGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKK
                     PVVSE"
BASE COUNT          190 a          197 c          275 g          300 t
ORIGIN      
        1 ccttttctcc tggccgggac tgggatttgt cggggcaggg ctgggtttaa ccatttctcg
       61 ttatattggc gcagttgcaa ttttgtgggt gctggcgatt ggttttaatc ctgcgctaag
      121 gatttcgtta aagagctatt ttaaaccgct gaattttagc attatctggg aagtcatggg
      181 gattggtatt cccgcgagtg tcgaatcagt gttatttacc agtggtcggt tattaaccca
      241 aatgttcgtt gccgggatgg ggaccagtgt tattgccgga aattttatcg cgttttcaat
      301 tgcggctctt atcaacttac ccggaagtgc gctcggctct gcttctacga tcattacagg
      361 ccgaaggttg ggggtagggc agatagcgca agcagagatt cagttgcggc atgtgttctg
      421 gctttccact cttggattaa cggccatcgc ctggctaacg gctccctttg ccggggttat
      481 ggcatcgttt tacacccagg atccacaggt taaacatgtc gttgtgattc tgatttggct
      541 aaatgcttta tttatgccta tttggtccgc ctcatgggtg ctacccgctg gatttaaagg
      601 tgctcgcgat gcccgttacg ccatgtgggt ttctatgttg agcatgtggg gttgccgggt
      661 tgtcgtcggt tatgtgctgg gcatcatgct tggctggggt gtggttggtg tctggatggg
      721 aatgtttgcc gactgggctg tgcgggccgt gctgttttac tggcgaatgg ttactggacg
      781 ttggctgtgg aaataccctc gatccgaacc gcaaaagtgt gaaaaaaagc cagttgtgtc
      841 ggaataaacg acaaaatgca gattatttca gcaaacgatt tcaaatttaa aaaacaggct
      901 ttgacattgt gggtgggcat cgctaatatt cgcctcgttc tcacgattcc tctgtagttc
      961 ag
//
LOCUS       DAZSBF010000510          959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZSBF010000510 DAZSBF010000000
VERSION     DAZSBF010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.518"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..507
                     /locus_tag="ACHXWD_004612"
     CDS             <1..507
                     /locus_tag="ACHXWD_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7623246.1"
                     /translation="KIHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNF
                     NNKILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDT
                     IDGLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTL
                     SYREVNRD"
     gene            500..>959
                     /gene="yqeJ"
                     /locus_tag="ACHXWD_004613"
     CDS             500..>959
                     /gene="yqeJ"
                     /locus_tag="ACHXWD_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR7623247.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNR"
BASE COUNT          355 a          157 c          173 g          274 t
ORIGIN      
        1 aagatccata atgacattaa catacaaaat catgtaataa actcgtcaac agacgcacat
       61 acacacaatg ccccacctgc cataaaagtt aatgcgggat acaaagagag cattggtggc
      121 gcaaagaatt tcaataacaa aatcctcaaa catataaaaa gtcatctaat tatgttgagc
      181 gcatttgtca taggcgcata ttctgcatat tggctatgga ataataatca accgaagcca
      241 tttttcaagg attacaagac tgttgcggaa attaatggct gccattttaa tgtaacagaa
      301 gatacaattg atgggttgaa agagttcgat aaatataaaa cacggatact ggattcgggg
      361 attaactgta aaaaatatcc gtggttatac ttccctcttg caaaatcctc accggggatg
      421 attgttatgg catgcaataa aaactataac caacatgaag tggcagactg tttaaccctg
      481 tcttaccgtg aggttaatcg tgattgatta taaaaaaaac ttattattta ttttagtttt
      541 tatcagtggg tttattctct tcactgtcta ttcttacact gcagaaaaaa tgatatataa
      601 cgaaacatgc actgcaaatt gggtaatttt taatgaccag ggacgagcaa atcttaccat
      661 cgactttatg tataacaaaa aaaataaaac aggaactgtt gcgttgagtg gaacctggca
      721 acaaggtaac agagaaagta aatcaatacg gcgaaatatt gaatacacat ggattgaaaa
      781 ctatgacaca gcccatttaa catcaaagaa agttaataag tttgagatta tggatcaagt
      841 tgatgatgat agacttgcac aacttattcc tgatttttat gtctttccag aaaaaagtgt
      901 aagctataat atactaaagc aaggtaagca tgcttttatt ttgagcattg gtaacagag
//
LOCUS       DAZSBF010000511          959 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZSBF010000511 DAZSBF010000000
VERSION     DAZSBF010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.519"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>959
                     /locus_tag="ACHXWD_004614"
     CDS             <1..>959
                     /locus_tag="ACHXWD_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HFR7623248.1"
                     /translation="ILGYQANSNGKVIVEKGGEWLIKNNDSSIEFQIGNQGAGEATIR
                     EGGLITAENTIIGGNATGIGTLNVQDQDSVITVRRLYNGYFGNGTVNISNNGLINNKE
                     YSLVGVQDGSHGVVNVTDKGHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIIT
                     AGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGV
                     GNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLM
                     DATSSFDTVGINVGNFGSGIVNVSNGATLNSTGYGFIGGNASG"
BASE COUNT          272 a          173 c          266 g          248 t
ORIGIN      
        1 cattttagga tatcaagcga acagtaatgg taaggttatc gttgaaaagg gtggcgagtg
       61 gctaataaaa aataatgatt cctcaattga atttcaaatt ggtaatcaag gagctgggga
      121 ggcgactatt cgcgagggtg ggctgattac ggctgaaaat acgattatcg gtggcaatgc
      181 caccggtatc ggaaccctga atgtgcagga tcaagactct gtcatcacgg tacgcagact
      241 ctataatggg tatttcggta atggcacagt caatatttcc aataatggtc tgattaataa
      301 caaagaatat tcattggtgg gcgttcagga cggttcccac ggtgtcgtca acgtgaccga
      361 taaagggcat tggagtttcc tcggaacagg cgaagctttc cgctatatct atatcggtga
      421 tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa gtagattcgg gaattatcac
      481 tgcggggatg aaagaaacag gcacaggcaa cattactgtt aaggataaga actccgttat
      541 cactaatctc ggaactaatc ttggttatga cggccacggc gaaatgaata tcagtaatga
      601 ggggcttgtt gtcagcaacg gaggaagctc actcggttat ggagaaaacg gcgtcgggaa
      661 tgtcagcatc accacggggg ggatgtggga ggtcaataag aatgtctata ccaccattgg
      721 tgttgcgggc gtcggaaacc tcaatattag cgatggcggt aagtttgtat cgcaaaatat
      781 tacttttttg ggcgataaag caagcggtat cggcacactg aacctgatgg atgcgacatc
      841 atcgttcgat actgtgggta tcaatgtcgg taattttggt agcggtatcg taaatgtcag
      901 taatggtgcc acccttaatt caacgggcta tggatttatc ggaggaaatg cctccggta
//
LOCUS       DAZSBF010000512          957 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZSBF010000512 DAZSBF010000000
VERSION     DAZSBF010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.520"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..395
                     /locus_tag="ACHXWD_004615"
     CDS             <1..395
                     /locus_tag="ACHXWD_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR7623249.1"
                     /translation="INAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSLAA
                     VLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVLD
                     GKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            569..>957
                     /locus_tag="ACHXWD_004616"
     CDS             569..>957
                     /locus_tag="ACHXWD_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR7623250.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          327 a          177 c          188 g          265 t
ORIGIN      
        1 tgattaatgc cattgtcaaa ccgttctatg aacgtcagat gagtaagtca gataatccaa
       61 ttgataaagt tgtaaggtca aaggtggaac ccacagacca agcattgact ggtgacatga
      121 actctttagc cgctgtttta tgggctatgg ttcagggcaa atcaaaagat gttccacagt
      181 tttcacctga agcattaaat aatctgaaag gtcgagtgga aacattcaca caagatttaa
      241 aggttatcaa accgtcagta aatattcagc ctcagacaat caatatcaca actcagacgg
      301 tacttgatgg aaaggttatt gatgaaagaa ccacatcgca tattaatcgt atgcaggaag
      361 ataccttgat tagctcagct tatccagagg aataaacaat gttagagcta ctaatattta
      421 ttgccacaca tggcaatcta ttaatcttta tctagtatga cgattcaact atgaatagtc
      481 gaatgctacg tgaaatcttc tgaacagata ccaacatcaa aaattagatt tcacgtagct
      541 cacgcttaac agaataggaa taacaacaat gtttgacaca aagcaaatta gatactcagg
      601 taaagatggt atctattttc accttagaga taatgtggaa tcttttcttg ccttaacagc
      661 tactgaacag atggactatg actcaccgat gatggtgaca actcaggcta tgcaatcggg
      721 gcaaactatc actgataatg ttcagagagc acctaagacc atctctatta gcggtgttgt
      781 agtggttgat tcgacgggtt cagttctatt aaccagacag gggcaacttg tagaaacctt
      841 tatttctacg gttgaacaat ggcgagacca gaagcaaatc atcactattg tttgtagaga
      901 tggaatagct atcactgata gtatcattac tcagtttaaa gcgagtaaag agcatac
//
LOCUS       DAZSBF010000513          953 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZSBF010000513 DAZSBF010000000
VERSION     DAZSBF010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.521"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..438
                     /locus_tag="ACHXWD_004617"
     CDS             <1..438
                     /locus_tag="ACHXWD_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623251.1"
                     /translation="TDNKKTETDAIYDKVRSSYLLSCILKKNKNVGLILHAPSFVSVS
                     EKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFVHKIKLTEAIRKL
                     RRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
     gene            548..706
                     /locus_tag="ACHXWD_004618"
     CDS             548..706
                     /locus_tag="ACHXWD_004618"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7623252.1"
                     /translation="MELQDWRKEPRKKYLVQIVGSFAEFERAMLRERTKSGLAAARQD
                     GLVDGDQS"
BASE COUNT          296 a          195 c          210 g          252 t
ORIGIN      
        1 actgataata aaaagacgga gacagatgcc atttacgata aagttcggag ttcttatttg
       61 ctttcatgta ttctgaaaaa aaacaaaaat gttgggctta ttcttcatgc accatcattt
      121 gtttctgtgt ctgaaaaaat cgccagaatt gtgatggcaa attactcccg taactggagt
      181 aattccgagt tggccagtgc ggttttaatg agtgaatctt ctttaaaaag aagaatgtac
      241 aaagaggtcg gttctataag tacatttgta cataaaataa agcttacaga agctatcagg
      301 aaattacgaa ggacaaatac tcccatttct gtcatatcat ctgagttagg ttacagttca
      361 ccatcgtatt tttctaaagt tttctttaaa tatttaaaga cataccctca aaacataagg
      421 aagaaaaatg gtcggtaatg tcaccaatta ggaggacttg cagagccgct tttttacgat
      481 actcaccccg taagtaagcg ataattggta acttaaacgc ccatctgaca tagatggaca
      541 cctgagtatg gaattacagg actggcgaaa agaacctcgt aaaaagtatt tggtgcaaat
      601 cgttggttcc tttgctgaat ttgaacgcgc catgcttcgt gaacggacaa agagtggact
      661 tgcagcggca cgccaggacg ggttggtgga tggcgaccaa agttaacacc ccagcaacaa
      721 aaagagattg tctcgctcgt cacatcgggg cagaaaacag gtgctgatgc cgcccgtctg
      781 tttcgggttc acccttccac cgtggtgcgt cttctcgcca gacaccggat gactgagatc
      841 aaacagacat aatctatgcg gagatttaca gcagggcatg gttcacaaat ctgacagcga
      901 tgaattatcg gcactgcggg cagaaaatgc tcgaatcatc aagcccctgt tac
//
LOCUS       DAZSBF010000514          950 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZSBF010000514 DAZSBF010000000
VERSION     DAZSBF010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.522"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            13..351
                     /gene="spa13"
                     /locus_tag="ACHXWD_004619"
                     /gene_synonym="spaM"
     CDS             13..351
                     /gene="spa13"
                     /locus_tag="ACHXWD_004619"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFR7623253.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            338..>950
                     /gene="spa32"
                     /locus_tag="ACHXWD_004620"
     CDS             338..>950
                     /gene="spa32"
                     /locus_tag="ACHXWD_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFR7623254.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKS"
BASE COUNT          374 a          128 c          178 g          270 t
ORIGIN      
        1 ttgtatcgag atgtggaggc attagataaa aggattatat acttcctgca actggagaat
       61 gacttagaac ctgtaggcgc tcagagtgtt agtcagcttt tcaatacaag acgtaagatc
      121 gctatagtaa aaaagcatat tatccaatat cagtctgaac gaattcttct aaagggaaga
      181 attgaagaaa ttcaaaaaga tattgatgag gcaaatgctt ctaaaagaaa actgcttcat
      241 aaagagagta aaatatgtaa acggattggc ttgattaaaa gaaataactt tgccaaacag
      301 ttgatacttg atgagttatc acaagaggat atgaagtatg gcattagata atataaacct
      361 aaattttagt agtgacaaac agatagaaaa atgtgagaaa ctatcttcaa tagataatat
      421 tgatagtctc gttttgaaga agaagaggaa ggttgaaatt cctgagtact ctttaattgc
      481 atctaactat tttactattg ataagcactt tgaacataag catgataaag gagaaattta
      541 tagtggcatt aaaaatgcgt tcgaacttag aaacgaacga gcgacatatt ctgatattcc
      601 agaatcaatg gccattaaag aaaatatttt gataccagat caagatataa aagcaaggga
      661 aaaaataaat atcggcgata tgagggggat cttttcatat aataagagtg gaaatgcaga
      721 caagaacttc gaaagaagtc atacttcttc tgtaaaccct gataatctgc tagaatctga
      781 taatagaaat ggtcaaattg gtttaaaaaa tcatagcttg tctattgata agaatattgc
      841 tgacatcatt tctttactaa atggaagtgt tgctaaatca tttgagctgc ctgtaatgaa
      901 taaaaatact gcagacataa ccccatccat gtcattgcaa gaaaaatcaa
//
LOCUS       DAZSBF010000515          947 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZSBF010000515 DAZSBF010000000
VERSION     DAZSBF010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.523"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(119..268)
                     /locus_tag="ACHXWD_004621"
                     /pseudo
     CDS             complement(119..268)
                     /locus_tag="ACHXWD_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            344..>947
                     /gene="ospD3"
                     /locus_tag="ACHXWD_004622"
     CDS             344..>947
                     /gene="ospD3"
                     /locus_tag="ACHXWD_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFR7623255.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLD"
BASE COUNT          324 a          163 c          174 g          286 t
ORIGIN      
        1 aataaatata tttaatcaaa attgaatatc gtttagcaaa aggcagtatt tgcacaaaat
       61 atcgatatat tgtttattgt cagtatggct caatgtgata atagttggaa agtttgatgg
      121 gtttcgcccc gttgtagcgg tagtcgagct ggaaggtctt caggcactgc ttacagcgat
      181 agagcagccc ccctgaactg gaatggccgt tccggatacc cccctgagtt tcagagtaac
      241 ggggacaaac cacatcaatc tttgccatca atcatccaaa gggcaaagag tacaacaaca
      301 ctaagtctgc gtcacaaccc atcaatgaaa ggaatatata catatgccat cagtaaattt
      361 aattccatca aggaaaatat gtttgcaaaa tatgataaat aaagacaacg tctctgttga
      421 gacaatccag tctctattgc actcaaaaca attgccatat ttttctgaca agaggagttt
      481 tttattaaat ctaaattgcc aagttaccga tcactctgga agacttattg tctgtcgaca
      541 tttagcttcc tactggatag cacagtttaa caaaagtagt ggtcacgtgg attatcatca
      601 ctttgctttt ccggatgaaa ttaaaaatta tgtttcagtg agtgaagaag aaaaggctat
      661 taatgtgcct gctattattt attttgttga aaacggttca tggggagata ttatttttta
      721 tattttcaat gaaatgattt ttcattccga aaaaagcaga gcactagaaa taagtacatc
      781 aaatcacaat atggcattag gcttgaagat taaagaaact aaaaatgggg gggattttgt
      841 cattcagctt tatgatccca accatacagc aactcattta cgagcagagt ttaacaaatt
      901 taacttagct aaaataaaaa aactgactgt agataatttt cttgatg
//
LOCUS       DAZSBF010000516          945 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZSBF010000516 DAZSBF010000000
VERSION     DAZSBF010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.524"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..945
                     /locus_tag="ACHXWD_004623"
                     /pseudo
     CDS             1..945
                     /locus_tag="ACHXWD_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          234 a          233 c          286 g          192 t
ORIGIN      
        1 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
       61 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      121 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc
      181 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg
      241 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      301 gagagctgcc cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac
      361 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gatcatgcgc cagaatgcgc
      421 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      481 ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg
      541 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
      601 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      661 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      721 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      781 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      841 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      901 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgc
//
LOCUS       DAZSBF010000517          933 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZSBF010000517 DAZSBF010000000
VERSION     DAZSBF010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.525"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(195..>933)
                     /locus_tag="ACHXWD_004624"
     CDS             complement(195..>933)
                     /locus_tag="ACHXWD_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR7623256.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          257 t
ORIGIN      
        1 gcttctccga taaaatgtca gagaagcatg attacgtggt tattgtctgt tgatgacttg
       61 ttgaccgagc cgtcatcatt ctcaatgcat tagttaaatc atttgtgatt tttaaggatt
      121 atcctgtcta tgattttcta cttagaagga tatggcacta actatgcatc ttatagttta
      181 ctgctggcat ctatttaaaa tagcaattga gtcaaatagc ccatcaagat agccatacct
      241 aatataaccg tcaggaatgc agctatcatc ggcattctga acattgattt aagtaatatc
      301 acttccgtca ggcttgcgcc ggcactgccg atgattaatg ccattaatgc tcccagaccc
      361 attccttttg tcatcagaac agatgccaga gggataaccg cctcagcccg gatatacagc
      421 ggaataccga caacggcgct taatgggatg gcgaagggat tatctgctcc tgcatgagcg
      481 gcaatccacg ctgagggaat aaagccataa ataaaagatc ctattaatac tcctaacaaa
      541 agatatggca gtacatcttt aaactgttgc aacgcatctt tggtggctag ttttatggga
      601 ttaatactta ctacagcttt ggtactgcaa caggtaacta cggggttctc aatggcttta
      661 gccgctggac tacaacaact cacttttatg ggcgtatatg tcgttcccgg cgaagttttt
      721 gctggagcac aacaatttgc tgatgagctt ttactggcaa tgatatgacg ttcaaacccc
      781 agagaatcca ggataatact ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac
      841 aaggtgactt tccaaccaaa ggtcatccac attagtccga cgataatggg attaagtaat
      901 ggtgaaacaa ataaaaaagt gagagtcgga cca
//
LOCUS       DAZSBF010000518          917 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZSBF010000518 DAZSBF010000000
VERSION     DAZSBF010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.526"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHXWD_004625"
     CDS             complement(<1..297)
                     /gene="lpfB-O113"
                     /locus_tag="ACHXWD_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR7623257.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPS"
     gene            complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHXWD_004626"
     CDS             complement(345..917)
                     /gene="lpfA-O113"
                     /locus_tag="ACHXWD_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR7623258.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          260 a          216 c          189 g          252 t
ORIGIN      
        1 cgaaggttct aatcgtgata ctggcggaat aacggtaaac ggcgctttgc ttttatcctc
       61 tttattaaat ggatctaccc aagcctggat gagataaggt attttttctt ctttgctaaa
      121 tacagaaata gatttatcag cattattact taagtaaacc acccgcgtac caccaatgac
      181 aacgcctgca taagttaatg ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa
      241 taacttacat gaaactgttg ttttaaagag cctgattaaa caactttttc tggacataac
      301 gactccctgg ccaaagccaa ctgaataaaa aggcccttga taaattattt cttatattcg
      361 acagtaaatt gtgaatcagc gttcgccgta ccagccgtta cggtcgcatt ggttgccaca
      421 tagcgtgcaa taaacttcat ttcagccttg ccttcagcat cgatagcggt ataagcagag
      481 tcgttataaa gtttaacctg cgaattatcg gactggttaa aaatacgaat accaacgccg
      541 gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc
      601 gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg taccatcaaa acgaacggct
      661 gcttgagtgt acgttgccgg gcaattttca agtttaatac tgaacttctg tggcgacgcc
      721 agactaccta cacctgaaaa tgcattttta ctgactttac ccagtgggac aatttgatcg
      781 gctgtatcgg aggtaacttc acaagtagtg tcgacaattt caccgacgaa gtgaacaaca
      841 ccatcttctg ccagtgcatg cccggccagc attagagatg ccagagtgaa tgcaccgcct
      901 ataatattac gcttcat
//
LOCUS       DAZSBF010000519          897 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZSBF010000519 DAZSBF010000000
VERSION     DAZSBF010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.527"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHXWD_004627"
     CDS             <1..386
                     /locus_tag="ACHXWD_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623259.1"
                     /translation="FLSLDELNDFLSMNESDLEVSSKKTSKQDREITDVSVDSDHKNT
                     QCPATDDELDPFDDEEDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDDYYLSP
                     KKKQTQDDKKQKIKPKMYFAGGGIK"
     gene            386..676
                     /locus_tag="ACHXWD_004628"
     CDS             386..676
                     /locus_tag="ACHXWD_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623260.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
BASE COUNT          320 a          152 c          186 g          239 t
ORIGIN      
        1 actttctttc tttggatgaa ttaaatgatt ttctctctat gaatgaatct gaccttgaag
       61 taagctctaa aaagacttct aaacaagaca gagaaatcac tgatgtgagt gttgatagtg
      121 accataaaaa cacacagtgc cccgccactg atgatgagtt ggaccccttc gatgatgaag
      181 aggatgaacg tgaaaaagcc attaagaggg cagagaagaa aaagagattc gagcttgcaa
      241 gacaaaagaa caaagtatcg ggtttagacc cattcgatga cagtgaagat gattattatc
      301 tttcaccgaa aaagaaacaa actcaggatg acaagaagca aaaaattaaa ccgaagatgt
      361 attttgccgg tggaggaatt aagtaatgaa agaatatcac ggtgaaaaga gatacaaaga
      421 ttacctgtta aagcgttaca gcatttctcg tgaaggtcat cttatgaagg atactcacgg
      481 ggaagtttac cggattagac caaagaaaga agggaggggt tatttctttt acgatggtgt
      541 gacagattta aagattgacg ctttgaggtt tgcagtgctg tatcattttg atatctggaa
      601 ttcaatccac cagctacgat taaaagacgg tgaccccagc aacttaaagg ccaccaatat
      661 tatcacaaaa cgttagtttg ttttaccatc atcaatcgta attgtgattt catatttgaa
      721 aagcttaatg ctgaaattga atcgtgtact tctggcgata atcattttta aaagctgata
      781 cacaagaagc accagaaaca caaagcaacc taagcttgcg aatctgtgag attcagcaat
      841 tgtttcaaga aagcccatta attcccctta tttatcaaag ggaatgacct gtatcag
//
LOCUS       DAZSBF010000520          891 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZSBF010000520 DAZSBF010000000
VERSION     DAZSBF010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..891
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.528"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..429
                     /locus_tag="ACHXWD_004629"
                     /pseudo
     CDS             1..429
                     /locus_tag="ACHXWD_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          227 a          229 c          241 g          194 t
ORIGIN      
        1 cctgtggaaa aaatacggca gtggcgataa cggtactccc ggccgatagt gaatgacctg
       61 tttgcctggc ttgaagagca ggagccgtgc tgtccgcccg acgggccatt aaataaggcg
      121 attaactata ttctgaaccg gcgcgatgaa ctgagctgct tcctgggtga cggagcggtg
      181 cccctggaca acaatatctg cgagcgtgcc attcgcccgg ttgttatggg acggaaagcg
      241 tggctgttcg ccggttcact gatggccgga aaccgggcgg cacagataat gagccttctg
      301 taaacagcaa aacgtaatgg tctggagcca catgcctggc tgacggacgt cctgacgcgt
      361 ctgccggaat ggccggagga gcgactggca gagttgctgc ctcttgaggg atttaccttc
      421 accgggtgag tgatacctgc cgtcaggtgt tcgtgaaccg ggctataacc agcagtcggt
      481 tgttgaacgc ctgacaacag gaaatcagcc aggccagcaa ccacgcggac tgacagagaa
      541 tcagaacctg aatcagcctg aaatcatacc cggggcagta cttcatcacc agtcaccaat
      601 acccgtgtca gtacttcgcg gcctgacgcg atcacccgac tcagtacttc tgagcccgcc
      661 tccggtacca ttctctgtgg gcccgtacac aacaacagaa aagggggccc ggcccggggc
      721 gcaaccgtat tacacttccg gaaaatcgtt atacgcagaa aaatcggatc acgctgtgcc
      781 tgctttcatg taatttactt acggtgttat aagtatgcta attatcgtat aaaaaaatcg
      841 cagcagaaaa aattgtatta aaaacttata gcgaattaat ggtgtatttt t
//
LOCUS       DAZSBF010000521          887 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZSBF010000521 DAZSBF010000000
VERSION     DAZSBF010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.529"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            59..859
                     /locus_tag="ACHXWD_004630"
                     /pseudo
     CDS             59..859
                     /locus_tag="ACHXWD_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          255 a          171 c          214 g          247 t
ORIGIN      
        1 ttggataacg gcacttattg atatattcat gaagattata atcacaaggg aatacataat
       61 gaaaagaaaa gttctggcaa tgctggtccc agcgttatta gttgctggcg cagcaaatgc
      121 ggctgaaatt tataataaaa atggcaataa actggatctg tacggaaaag ttgatgctcg
      181 tcatactttt tccgataacc ctggcgatga cggcgatgaa actatcatcg agttaggctt
      241 caaaggtgag acccaaatta cagatcagtt aactggttat ggtcaagcac tgaccaaaac
      301 aaaggctagc gacactgaag gttctgataa tacttatgtc aaacttgctt ttgcaggtct
      361 gaagttcggc gagatgggct cctttgacta tggtcgtaac tatggtgtga tttacgacgt
      421 tgaagcctgg acagatatgc ttcctgtatt cggcggcgac tcctatacct ggactgataa
      481 cttcatggca ggccgtgcaa atggggtagc tacttaccgc aatagtgatt tcttcggtct
      541 ggtggaaggt ctgaactttg cattgcagta tcaaggtaac aacgaaggca gcaatgcagg
      601 agaagatcaa gaaggtacta aaaatggtca cgaagacgtt cgcttccaga acggtgatgg
      661 ttttggtctt tccacttcat atgactttga cttcggtttg agcttaggtg ccgcttactc
      721 aaactctgac cgtaccaatt ctcaagtagc tcttggcggg tatcactaca atgaatacag
      781 caaatttgct ggtggtgata cagctgaagc atggacattt ggtgcaaaat acgacgccaa
      841 caatgtttat cttgcaatgg gtgatgctgc caacttactg atttagt
//
LOCUS       DAZSBF010000522          875 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZSBF010000522 DAZSBF010000000
VERSION     DAZSBF010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.530"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>875
                     /gene="cadA"
                     /locus_tag="ACHXWD_004631"
     CDS             <1..>875
                     /gene="cadA"
                     /locus_tag="ACHXWD_004631"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR7623261.1"
                     /translation="FSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKG
                     DVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIK
                     RLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGM
                     EKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDF
                     KRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLP
                     TMVMTPYAAFQKELH"
BASE COUNT          237 a          220 c          212 g          206 t
ORIGIN      
        1 acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta gaagggaaag
       61 tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag gcttccatga
      121 tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg atgcacacca
      181 ccacttctcc tcactacggt atcgtggcgt ccactgaaac cgctgcggcg atgatgaaag
      241 gcaatgcagg taaacgtttg atcaacggtt ccattgaacg tgcgatcaaa ttccgtaaag
      301 agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtatgg cagccggatc
      361 atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac ggcttcaaaa
      421 acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg actccgggga
      481 tggaaaaaga cggcaccatg agcaactttg gtattccggc cagcatcgtg gcgaaatacc
      541 tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg ttcctgttca
      601 gcatcggtat cgataagacc aaagcactga gcctgctgcg tgctctgact gacttcaaac
      661 gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat cgtgaagatc
      721 ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac aaactgattg
      781 ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg actatggtaa
      841 tgactccgta tgctgcgttc cagaaagagc tgcac
//
LOCUS       DAZSBF010000523          850 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZSBF010000523 DAZSBF010000000
VERSION     DAZSBF010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.531"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..133)
                     /locus_tag="ACHXWD_004632"
     CDS             complement(<1..133)
                     /locus_tag="ACHXWD_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623262.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004633"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="ACHXWD_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          247 a          203 c          176 g          224 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggtgact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtgt tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      661 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      721 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa
      781 ctaatatcaa gcggcgctat attgcgtatt aaataaaacc acctcaatta cattaataaa
      841 tctaaacaaa
//
LOCUS       DAZSBF010000524          826 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZSBF010000524 DAZSBF010000000
VERSION     DAZSBF010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.532"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(1..177)
                     /locus_tag="ACHXWD_004634"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="ACHXWD_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            161..419
                     /locus_tag="ACHXWD_004635"
                     /pseudo
     CDS             161..419
                     /locus_tag="ACHXWD_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(528..>826)
                     /locus_tag="ACHXWD_004636"
     CDS             complement(528..>826)
                     /locus_tag="ACHXWD_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623263.1"
                     /translation="ITPGESGFVSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKY
                     ITDMCGLICMRHIESTGIIFYMLDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
BASE COUNT          241 a          200 c          153 g          232 t
ORIGIN      
        1 gctgtgctgc cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg
       61 cggatgttca ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa
      121 gcgatcgaag gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt
      181 gctgagccag tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt
      241 tattgtcctc aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata
      301 atctgaacct gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt
      361 ctgatgatat gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa
      421 tctgcgtcac gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg
      481 cgaacctctc gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta
      541 tataagttct ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca
      601 atggcagata aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc
      661 ctcatacaaa taaggccgca catatccgta atatatttgt caatgctgct ataatttgca
      721 tggtaatatg ccatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct
      781 tcacgattaa caaaactgac aaaaccactt tcaccaggag taatat
//
LOCUS       DAZSBF010000525          811 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZSBF010000525 DAZSBF010000000
VERSION     DAZSBF010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.533"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..811
                     /locus_tag="ACHXWD_004637"
                     /pseudo
     CDS             1..811
                     /locus_tag="ACHXWD_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAZSBF010000526          802 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZSBF010000526 DAZSBF010000000
VERSION     DAZSBF010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.534"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHXWD_004638"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="ACHXWD_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR7623264.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
BASE COUNT          232 a          129 c          198 g          243 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt ca
//
LOCUS       DAZSBF010000527          799 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZSBF010000527 DAZSBF010000000
VERSION     DAZSBF010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.535"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            229..>799
                     /locus_tag="ACHXWD_004639"
     CDS             229..>799
                     /locus_tag="ACHXWD_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR7623265.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLICLCY
                     SQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPMADFSS
                     VSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPRLNKLV
                     TEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          224 a          175 c          169 g          231 t
ORIGIN      
        1 cccaccggca gaagcagaaa aagcttatta tgcttccatc ggaaatgatg atctggcagc
       61 ctgagttcac agataaaaca ctctccagga aacccggggc ggttcagttc acaaaaacac
      121 attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca ttagcttctt
      181 cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt gaataaagtt
      241 tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga acttgcccgc
      301 agggtaataa aaaagacatg ccgaagatta acgcatattc ttctaatctg tctgtgttac
      361 tctcagatat ccctggcggg tattgtccgt tccgatattg cctatcagat ttatcgtgat
      421 ttcgccgaaa acaaagggct ttttgtacct ggtgccaatg atattccggt atatgataag
      481 gacggaaaac ttgtgggaag actgggtaaa gccccaatgg ccgatttcag cagtgtgagc
      541 tcaaatggcg ttgctacgct tgtatcgcct cagtatatcg tcagcgtaaa gcataacgga
      601 ggatatcgga gtgtgagctt tggtaatggg aaaaatacat attcccttgt tgaccgtaat
      661 aaccaccctt ctattgactt ccatgctcca cgtctgaata aactggttac agaagttatt
      721 ccctcagcgg taacatcaga aggaaccaaa gccaatgctt ataaatacac tgaacgttac
      781 accgcttttt atcgggtgg
//
LOCUS       DAZSBF010000528          795 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZSBF010000528 DAZSBF010000000
VERSION     DAZSBF010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.536"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHXWD_004640"
     CDS             complement(<1..741)
                     /locus_tag="ACHXWD_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR7623266.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          266 a          137 c          161 g          231 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaagttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattt
//
LOCUS       DAZSBF010000529          792 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZSBF010000529 DAZSBF010000000
VERSION     DAZSBF010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.537"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            185..751
                     /gene="slp"
                     /locus_tag="ACHXWD_004641"
     CDS             185..751
                     /gene="slp"
                     /locus_tag="ACHXWD_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR7623267.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          239 a          168 c          175 g          210 t
ORIGIN      
        1 ggattatatt atggggcgat ggatactcat gccacgcccc aaaatcaatg gaattgaact
       61 aaacaaaagg gatataaaca tcagacaggt ttacgttact atcaggcata tcgcctcaga
      121 atcagatgaa aactataaag aaatatctat tatggtttta atatttgttg ataaggatag
      181 taacatgaac atgacaaaag gtgcactcat cctcagcctt tcatttttgc ttgccgcatg
      241 tagttcaatt ccgcaaaata tcaaaggcaa taaccaacct gatattcaaa aaagttttgt
      301 tgctgttcat aaccagccgg ggttatatgt tggtcaacaa gcgcgctttg gtgggaaggt
      361 tatcaacgtt atcaatggca aaacggatac gttgttagaa atcgctgtat taccgttgga
      421 tagctatgcg aaacctgata ttgaagccaa ctatcagggc cgactgctcg ccagacaaag
      481 cggcttcctt gacccagtga actatcgtaa tcactttgtt accatcctcg gtaccattca
      541 gggtgaacaa cctggcttta tcaataaagt cccgtataac ttcctggaag tgaatatgca
      601 gggcatccag gtgtggcatt tgagagaagt ggttaatacc acctataacc tgtgggatta
      661 cggctatggt gcattctggc cggaaccggg ctggggtgcg ccttactaca ccaatgcggt
      721 gagtcaggta acacctgagc tggtcaaata acaccaccga aagatgcaga tgctttctca
      781 gcatctgcat ca
//
LOCUS       DAZSBF010000530          791 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZSBF010000530 DAZSBF010000000
VERSION     DAZSBF010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.538"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>791)
                     /locus_tag="ACHXWD_004642"
     CDS             complement(<1..>791)
                     /locus_tag="ACHXWD_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000909824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR7623268.1"
                     /translation="NINVVMCFGIICGVVKLFIGYSEDSNFIVYLNRNATAIIVVCFY
                     CVYSYFYRGRKSWYVSSVLYSLFFLFLDSRAGIISFAISLFFVFLQLTKKEKLLISLF
                     FVPLLTLGISFTDIGTRLERMLSSSQVIFSGGNTLTKSQNDYRRVELVFIGVDVLKEN
                     YLIGTGLGVANYVKAIDKKFLGSTNFGLAHNFYLSYSAQLGIIGFILLISVFYIMLSP
                     IFKCGGYIGKGCVFALAFYVFFNEYILTPAIYIYISIFLSVVFIR"
BASE COUNT          348 a          149 c           90 g          204 t
ORIGIN      
        1 tacgtataaa aaccaccgat aaaaaaatag aaatataaat atatatcgct ggcgtcaata
       61 tatactcatt aaaaaagaca tagaaagcca aagcaaaaac gcatccttta ccaatatacc
      121 ctccgcattt aaaaattgga gacagcatta tataaaatac agaaataagc aaaataaaac
      181 caataatccc taactgagcc gaataagata aataaaaatt atgcgccaac ccaaagttgg
      241 tacttcctaa aaacttttta tctatagcct ttacataatt tgcaacacct aatccagtgc
      301 caattaaata attttctttt aaaacatcaa ccccaataaa tactaactca actcgacgat
      361 aatcattctg actttttgta agagtgttac caccagagaa tataacctgt gacgaagaca
      421 gcattcgttc aagacgagtg cctatatcag taaaagaaat acctaaagtt agaagaggaa
      481 caaaaaacaa tgatattaat aacttttcct tctttgttaa ctgaagaaaa acaaaaaaca
      541 acgatatagc aaatgatatt attcctgctc ggctatccag aaaaagaaag aacagagagt
      601 acaatacaga tgagacatac caagactttc gaccacgata aaagtatgaa tatacacaat
      661 aaaagcacac tactataatt gcggtggcat ttctatttaa ataaactata aaattactat
      721 cttcagaata accaataaat aattttacaa ctccacagat aataccaaaa cacattacaa
      781 catttatatt t
//
LOCUS       DAZSBF010000531          789 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZSBF010000531 DAZSBF010000000
VERSION     DAZSBF010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.539"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..274
                     /locus_tag="ACHXWD_004643"
     CDS             <1..274
                     /locus_tag="ACHXWD_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623269.1"
                     /translation="LNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKE
                     EARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            286..735
                     /locus_tag="ACHXWD_004644"
     CDS             286..735
                     /locus_tag="ACHXWD_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR7623270.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          263 a           88 c          196 g          242 t
ORIGIN      
        1 attaaactcg aaatcacaat cgcctgcatt aggtttggga catgagttta ctcatgcgca
       61 gtattgcctt cttgataagg aaaatttcat ggctctttta agccgaacag ataagaaata
      121 tgagaataaa gaagaggcaa gggttattac aataattgaa tcgcgtgcgg cgaaaacttt
      181 aggtgagtgt accagaggag ctcatagtgg actgcctttt tatcgggtgg atggaccgtt
      241 gcagacgatg aaaataacgg gtacgccaga atagtgaggt tttttatgat gaaatatttg
      301 atggttttat tgtctctatt ttctggttcg gtattgggga tggggcgagt gaatgaattg
      361 tgtggaatag atagcgtgaa aacgatagag ataattaatc ttccatcgta tgttaccacg
      421 ttagttccgc ttagcaagga aggtcttaat gaaatataca gatataaagt ggtggtgaat
      481 gaaattagag atctttatgc tggtaagata attgatttat tgcaaatgaa gtatttcaga
      541 aaagaaaaat ataacaatat aagatggggg ggtagtatca tttcgaaagg taataataaa
      601 tgtgaaattt attttgatgc ttttggtgaa tgtggaagtg tgaatggcat taacgtgtgt
      661 tttgaaaaaa atgaaatgat aggttggatt aaaaaagaaa tccctttgct gtcgcaaaaa
      721 ataggtgggc tttaaatggt agggcaatat tcaaagacag tataggtaac tatttgtatg
      781 gccatgtaa
//
LOCUS       DAZSBF010000532          788 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZSBF010000532 DAZSBF010000000
VERSION     DAZSBF010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.540"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            211..>788
                     /locus_tag="ACHXWD_004645"
     CDS             211..>788
                     /locus_tag="ACHXWD_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR7623271.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          172 a          237 c          205 g          174 t
ORIGIN      
        1 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
       61 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
      121 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
      181 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cgaatccgtc
      241 caggtcttta tcggcgttga tgtcggtaaa gatacgcatc acgctgttgc cattaatcgt
      301 tcaggtaaac gcctgttcga taaagcatta cccaacgacg aaaacaaact caggtcgcta
      361 atatctgacc tgaaacaaca tggtcagata ctgctggttg ttgatcagcc agctaccatc
      421 ggtgcgttac ctgtcgccgt tgcccgctca gaaggagtcc ttgtcggata cctccctgga
      481 ctggccatgc gccgcatagc cgacttacac gccggtgaag ctaaaactga tgctcgtgac
      541 gctgccatca ttgccgaagc tgcccgtacc ctgcctcacg cgctacgcac gctgaaactg
      601 gctgacgagc aaatcgccga actctccatg ctctgcggct tcgatgatga tcttgccgca
      661 cagacaacgc aggccagcaa ccgtatccgc ggccttctga cccagataca tccggcactg
      721 gagcgcgttc tcggtccgag acttgagcac ccggcggtac tcgatcttct ccagcgatat
      781 ccctcacc
//
LOCUS       DAZSBF010000533          777 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZSBF010000533 DAZSBF010000000
VERSION     DAZSBF010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.541"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            48..764
                     /locus_tag="ACHXWD_004646"
     CDS             48..764
                     /locus_tag="ACHXWD_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR7623272.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          257 a          135 c          167 g          218 t
ORIGIN      
        1 taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat
       61 gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa
      121 aattcggtgt agaaatctat gctgctgcta aacaaggtga acccgatcca gaattaaaca
      181 catctttaaa attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg
      241 ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag ggacgttatg
      301 aaggctttgg tcctaatggc tcaatgatta tcgccgagac attgacttca aatgttaacc
      361 gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag
      421 gttctgtcag ctatatgttt gacaatgctg gtgtgattgt atttaaagga tcagaccctg
      481 accatatttt tgaaatttta cttgaagctg aagttgatgt tcgtgatgtg actgaagaag
      541 aaggtaacat tgttatttat accgaaccta ctgaccttca taaaggaatc gcggctctaa
      601 aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag
      661 ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg
      721 acgacgatgt acaaaaagtt tatcataacg tcgcaaatct ctaattatct tttaaag
//
LOCUS       DAZSBF010000534          775 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZSBF010000534 DAZSBF010000000
VERSION     DAZSBF010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.542"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHXWD_004647"
     CDS             <1..343
                     /locus_tag="ACHXWD_004647"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR7623273.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="ACHXWD_004648"
     CDS             complement(405..>775)
                     /locus_tag="ACHXWD_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR7623274.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAZSBF010000535          774 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.543, whole genome shotgun sequence.
ACCESSION   DAZSBF010000535 DAZSBF010000000
VERSION     DAZSBF010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.543"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..349
                     /locus_tag="ACHXWD_004649"
     CDS             <1..349
                     /locus_tag="ACHXWD_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein mxiK"
                     /protein_id="HFR7623275.1"
                     /translation="SGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGKIS
                     KQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGMQY
                     AKRHFTGIQTSCL"
     gene            291..>774
                     /gene="mxiN"
                     /locus_tag="ACHXWD_004650"
     CDS             291..>774
                     /gene="mxiN"
                     /locus_tag="ACHXWD_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFR7623276.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     K"
BASE COUNT          266 a           97 c          175 g          236 t
ORIGIN      
        1 aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg ttgcttattt
       61 tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta gttattatgg
      121 gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc atcttattgc
      181 gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat ttaatccaat
      241 tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa atgaaggtat
      301 gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat ggtgttgtta
      361 taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact gagagtaaga
      421 agaaagcaat ttgtgttata aaggatgcta cgaaaaaagc tgaatcactg cgtatagatg
      481 ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt attgactata
      541 tttgtgaatg gaagttaaag caaaatgaaa acagaagaaa tattgaggac tatataacta
      601 gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg gagcagtggc
      661 ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag tgtaatttag
      721 cactgaggaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc aaga
//
LOCUS       DAZSBF010000536          770 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.544, whole genome shotgun sequence.
ACCESSION   DAZSBF010000536 DAZSBF010000000
VERSION     DAZSBF010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..770
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.544"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHXWD_004651"
     CDS             complement(<1..304)
                     /locus_tag="ACHXWD_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR7623277.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          240 a          171 c          166 g          193 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc
//
LOCUS       DAZSBF010000537          764 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.545, whole genome shotgun sequence.
ACCESSION   DAZSBF010000537 DAZSBF010000000
VERSION     DAZSBF010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.545"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            26..764
                     /locus_tag="ACHXWD_004652"
                     /pseudo
     CDS             26..764
                     /locus_tag="ACHXWD_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          221 a          187 c          201 g          155 t
ORIGIN      
        1 acctcagaac aacacaggtg ctccaatgaa aaaaagaaat tttagcgcag agtttaaacg
       61 cgaatccgct caactggttg ttgaccagaa atacacggtg gcagatgccg ccaaagctat
      121 ggatgttggc ctttccacaa tgacaagatg ggtcaaacaa ctgcgtgatg agcgtcaggg
      181 caaaacacca aaagcctctc cgataacacc agaacaaatc gaaatacgta agctgaggaa
      241 aaagctacaa cgcattgaaa tggagaatga aatattaaaa aaggctaccg cgctcttgat
      301 gtcagactcc ctgaacagtt ctcgataatc gggaaactca gagcgcatta tcctgtggtc
      361 acactctgcc atgtgttcgg ggttcatcgc agcagctaca gatactggaa aaaccgtcct
      421 gaaaaaccag acggcagacg ggctgtatta cgcagtcagg tacttgagct acatggcatc
      481 agccacggtt cggccggagc aagaagcatc gccacaatgg caacccggag aggctaccag
      541 atgggacgct ggcttgctgg caggctcatg aaagagctgg ggctggtcag ctgtcagcag
      601 ccgactcacc ggtataaacg tggtggtcat gaacatgttg ctatccctaa ctaccttgaa
      661 aggcagttcg ccgtgaccga gccaaatcag gtgtggtgcg gtgatgtgac ctatatctgg
      721 acgggtaagc gctgggcgta cctcgccgtt gttctcgacc tgtt
//
LOCUS       DAZSBF010000538          753 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.546, whole genome shotgun sequence.
ACCESSION   DAZSBF010000538 DAZSBF010000000
VERSION     DAZSBF010000538.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.546"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            74..>753
                     /locus_tag="ACHXWD_004653"
     CDS             74..>753
                     /locus_tag="ACHXWD_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7623278.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          235 a          161 c          130 g          227 t
ORIGIN      
        1 cctgaaacat tttatttcag cttattattt cccagtatca cattttatca gaatatcctt
       61 ctgcgaggtt attatgcttc ctgtaaataa tcccccccta tccactggaa acgtctcttt
      121 ttacagaact acatcaatcg acaatgttca caataattat ctctccgaat gggttgaatg
      181 gactaaaaac agcatttccg gagaaaacag ggaaactgct tttacccggc tccaattatg
      241 tctggagaac agtgaaacat cgttggactt atcttgttta ggtctcagat ctctaccacg
      301 attgcctgac aatcttgatg aaattaatgt aagcaataac caactatcaa tgctccccga
      361 gctaccaagg gcattgaaag agctgaatgc aagcagtaat caattatctg cacttcctga
      421 attaccagtg tcgctggaat atataaatgt gagtgataac catttgttcg cacttcctga
      481 attacctgcg tcactagaat atattaatgt aagtgacaat cacctgtctg tacttccgag
      541 gttaccaatg tcattggaat tacttgatgc agccagaaat gctttggaag taataccaga
      601 ttttccagaa agagatgatc atattataag aatattctgg cttaatcaga accggatcac
      661 ggcaattccg gaaagcatac ttggcctcag ttctgatagc gttgtcaatc ttagagaaaa
      721 tcaactatct cccagaataa tgcaaacttt gtt
//
LOCUS       DAZSBF010000539          749 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.547, whole genome shotgun sequence.
ACCESSION   DAZSBF010000539 DAZSBF010000000
VERSION     DAZSBF010000539.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.547"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            584..>749
                     /locus_tag="ACHXWD_004654"
     CDS             584..>749
                     /locus_tag="ACHXWD_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR7623279.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVI"
BASE COUNT          212 a          135 c          164 g          238 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcg
//
LOCUS       DAZSBF010000540          745 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.548, whole genome shotgun sequence.
ACCESSION   DAZSBF010000540 DAZSBF010000000
VERSION     DAZSBF010000540.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.548"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..723
                     /locus_tag="ACHXWD_004655"
     CDS             <1..723
                     /locus_tag="ACHXWD_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR7623280.1"
                     /translation="SNNLSWFIYKYDELAELAAVIDRLYEFHQLTEQRPTNKPKNCQH
                     AVQVANASIRTPDNKIILENLNFHVSPGKWLLLKGYSGAGKTTLLKTLSHCWPWFKGD
                     ISSPADSWYVSQTPLIKSGLLKEIICKALPLPVDDKSLSEVLHQVGLGKLAARIHDHD
                     RWGDILSSGEKQRIALARLILRRPKWIFLDETTSHLEEQEAIRLLRLVREKLPTSGVI
                     MVTHQPGVWNLADDICDISTVL"
BASE COUNT          214 a          163 c          164 g          204 t
ORIGIN      
        1 tcgaacaatt taagctggtt tatttataaa tatgacgaac ttgctgaact ggctgcggtt
       61 atcgatcgct tgtatgagtt ccatcaactc actgaacagc gccctacgaa taagcctaaa
      121 aattgccaac atgcggtaca agtggctaat gcgagtattc gtacgcctga taataagatc
      181 atattagaga acttgaactt tcatgtttcg ccaggcaaat ggctattact gaaaggctac
      241 tctggtgcgg gaaaaaccac actgcttaaa acattatccc actgctggcc gtggtttaaa
      301 ggtgatattt cttctcctgc tgacagttgg tatgtgtcac aaacaccgtt aatcaaaagc
      361 ggcttactga aagagattat ttgtaaagca cttcccctgc ccgtagacga taaatcgttg
      421 agcgaagtac tgcatcaggt tggtctgggg aaattggctg cgcgtattca tgaccacgat
      481 cgctggggag atattctttc cagcggcgaa aaacaacgta tcgccctggc acgattaatt
      541 ttacgacgtc cgaaatggat atttcttgac gaaactacct ctcatcttga ggaacaagag
      601 gctatacgct tactgcgttt agtgcgtgaa aaactaccca caagcggcgt cattatggtt
      661 acacatcaac ccggtgtctg gaacctggct gatgatattt gtgacattag cacggtttta
      721 taacaacaat aaaagccctg ctgta
//
LOCUS       DAZSBF010000541          744 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.549, whole genome shotgun sequence.
ACCESSION   DAZSBF010000541 DAZSBF010000000
VERSION     DAZSBF010000541.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.549"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>744
                     /locus_tag="ACHXWD_004656"
     CDS             <1..>744
                     /locus_tag="ACHXWD_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR7623281.1"
                     /translation="NLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAP
                     VAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGP
                     TGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINE
                     MDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNI
                     EEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT          236 a          160 c          174 g          174 t
ORIGIN      
        1 gaatttatta tcttccgcta ttatagtcgc catcatggcc ctcggtctga cgggttgtga
       61 tgataaaaaa gccgaaacag aaacgccccc gcctgccaat agtcaacctg ccgcaccagc
      121 tcctgaagct cccgttgcaa aagcagaagc taaacctgaa acacctgcgc aaccggtggt
      181 cgatgaacaa gcggttttcg acgaaaaaat ggatgtctat atcaagtgct acaacaagtt
      241 acagatccct gtacagaaca gtctggcgcg ttatgctgac tggctgaaag attttaaaca
      301 ggggcctacc ggtaaagaga gtactgttta tggcatctac ggcattagtg aatccaacct
      361 cgctgagtgt gaaaaaggcg taaaaagtgc tgtggcgtta acgcctgcgc tgcaacctat
      421 tgatggcgtg gcggtgagtt atattgatgc tgccgtggcg ttgggtaata ccattaacga
      481 aatggataaa tattacaccc aggaaaatta taaagacgat gcgtttgcga aagggaaaac
      541 gctgcaccag acattcttaa aaaatctgga atcctttgaa cctgtagcag aatcttatca
      601 tgcggcgatt caggaaatta atgataagcg ccagcttgcg gaactgaaaa atattgaaga
      661 aagagaaggg aaaacattcc actactactc tctggcagtc atgatttcag cgaaacaaat
      721 taataacctg atttcgcaag agaa
//
LOCUS       DAZSBF010000542          743 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.550, whole genome shotgun sequence.
ACCESSION   DAZSBF010000542 DAZSBF010000000
VERSION     DAZSBF010000542.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.550"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHXWD_004657"
     CDS             complement(<1..396)
                     /locus_tag="ACHXWD_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7623282.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          172 c          108 g          230 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcattcatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAZSBF010000543          743 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.551, whole genome shotgun sequence.
ACCESSION   DAZSBF010000543 DAZSBF010000000
VERSION     DAZSBF010000543.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.551"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>743
                     /locus_tag="ACHXWD_004658"
     CDS             <1..>743
                     /locus_tag="ACHXWD_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR7623283.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETID"
BASE COUNT          184 a          171 c          254 g          134 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag att
//
LOCUS       DAZSBF010000544          739 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.552, whole genome shotgun sequence.
ACCESSION   DAZSBF010000544 DAZSBF010000000
VERSION     DAZSBF010000544.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.552"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            121..>739
                     /gene="virB"
                     /locus_tag="ACHXWD_004659"
     CDS             121..>739
                     /gene="virB"
                     /locus_tag="ACHXWD_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFR7623284.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKA"
BASE COUNT          275 a          134 c          128 g          202 t
ORIGIN      
        1 gctattaaaa tagtaatttt taagactacc gttgactatc atcaacagtt acatacaatc
       61 ttgctcacac tgcatttaac ttttgtcaat ataaatcaat atgaataaac agggtgtgat
      121 atggtggatt tgtgcaacga cttgttaagt ataaaggaag gccaaaagaa agagtttaca
      181 ctccattctg gtaataaagt ttcctttatc aaagccaaga ttcctcataa aaggatccaa
      241 gatttaacct tcgtcaacca aaaaacgaat gtacgcgatc aagaatccct aacagaagaa
      301 tcattagccg atatcataaa aactataaag ctacaacaat tcttccctgt aataggaagg
      361 gagattgatg gtagaattga aattctggat ggcactcgta gaagagcatc tgcaatatat
      421 gcaggagcag atcttgaagt tctatattca aaagaatata tatctactct tgatgccaga
      481 aaactagcaa acgatataca aacagcaaaa gagcatagca tccgagaact tggtatcggt
      541 cttaattttc tgaaagtatc agggatgtcc tataaagaca tagccaaaaa agagaatctg
      601 tctcgcgcga aagtcactcg tgcctttcag gcagcaagcg ttccacagga aataatatct
      661 ctatttccaa tcgcgtcaga acttaacttt aatgactaca agatattatt caattattat
      721 aaaggacttg aaaaggcta
//
LOCUS       DAZSBF010000545          732 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.553, whole genome shotgun sequence.
ACCESSION   DAZSBF010000545 DAZSBF010000000
VERSION     DAZSBF010000545.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.553"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          224 a          165 c          138 g          205 t
ORIGIN      
        1 atcctgtgaa gtaaacggcg acggctcaaa attttatcaa acctaagata attctaccat
       61 caaagttaat aatttgaatt tatatgacgg ctctatgggg gggtaaaaat ccccgtgcag
      121 agaggcgatt ttcagagttg aaaacgtcgt cgttaagaac accaaagtca gtaccaccaa
      181 cccggaaagt agcttcatgc accgggacaa cagcagtttt ggtgtccgaa atcgtacaat
      241 tctgtacatc tacgcaagga aatgtacgga agtaagccaa atggttatcg gacggtcgga
      301 acatttcgta cagcctttac gttcagtgag ttagctggcc tagatgtaac cactcgttca
      361 actcaattct cttagcgtgg gaaaatttcc gttttccaag cggccccctc aagcggaccc
      421 cttatagtac aataattttc gatatccaaa ctgcccccaa ttacatctcc agtctctggc
      481 agcagagatc atatatttcc attgccgccg tagccacgat attctggcta ttcacaaaaa
      541 caatttagcc ccaaagcgca gtaaaaaagt acactatgaa ggtgtatgat accttcatag
      601 tgcttaagta attatgagta atataatctg tgtaaactac taattgcttc accgtcagtg
      661 attaaatgcg tagctgttcc ttgtcttata taatacctat tttcaaaatg acagacttct
      721 ccgacacttt tt
//
LOCUS       DAZSBF010000546          731 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.554, whole genome shotgun sequence.
ACCESSION   DAZSBF010000546 DAZSBF010000000
VERSION     DAZSBF010000546.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.554"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..239
                     /locus_tag="ACHXWD_004660"
     CDS             <1..239
                     /locus_tag="ACHXWD_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR7623285.1"
                     /translation="ADRNRSHWSTSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            344..535
                     /locus_tag="ACHXWD_004661"
     CDS             344..535
                     /locus_tag="ACHXWD_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR7623286.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(536..>731)
                     /locus_tag="ACHXWD_004662"
     CDS             complement(536..>731)
                     /locus_tag="ACHXWD_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR7623287.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          189 a          185 c          161 g          196 t
ORIGIN      
        1 cagctgatag aaacagaagc cactggagca cctcaaaaac accatcatac actaaatcag
       61 taagttggca gcatcacccg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
      121 aactcgatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
      181 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
      241 ccaaaaagaa cccgtcggga tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
      301 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
      361 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
      421 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
      481 tgaggatttt atagatggtt tctggcgacc tgcacaggag tacggtgatt tttaattatt
      541 gcaattgcac aagagtcagt tcgcccccaa agacagcacc ggtatcaata taatgcaggt
      601 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctg
      661 taattccctg ccctttttgg cgttcaccta atcgcgagcg gctccacaag acctgatgca
      721 aatcaacgtc c
//
LOCUS       DAZSBF010000547          730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.555, whole genome shotgun sequence.
ACCESSION   DAZSBF010000547 DAZSBF010000000
VERSION     DAZSBF010000547.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.555"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>730)
                     /locus_tag="ACHXWD_004663"
     CDS             complement(<1..>730)
                     /locus_tag="ACHXWD_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR7623288.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAF"
BASE COUNT          194 a          194 c          155 g          187 t
ORIGIN      
        1 aaaaaggcaa acagatccga agcgggataa tctttttggg gagaattgtt ccagttagca
       61 atatatcccg acagggggtt atacacctta gggttcattt caaaaggcaa tagccctttc
      121 cagtcccatt ttcccgtacc aggaacgggt aatcgcggat catggcctga ttgacgatct
      181 ggataagcac cagtatgaac ataaccaata ttgccgttta catcagcata gtaccagttg
      241 atggtcagtg cttgtttcgc tgcctgctgt gtccactcct gccaattttt ggccttcatc
      301 tgatgagtcc aggccagcaa agacgccacc tctttaccat cccatgcgcg ggatttagcg
      361 taagccgttt gtgtcgtctg gtcagtttgg agaatgttgc catgcaccgt acgccagaca
      421 gtaaaggtct ctgcctgacc atttttcacc gtaatggttt cctcacggct taacattttc
      481 acccacttac cattatgcaa gtagtagcct ggtttctctg ccaacagccg ttcagcaaaa
      541 atatcgacat catcgccgaa acctgccgtt gatccccagg aaatcacacc attatgacca
      601 aaaaccagcc caggataggc aaatggtgta ttgccagtga catcataacc agcaccgtgc
      661 agaccaatac cataagtata cgcaggcgca taccagccaa actgcggacc atttaccatg
      721 attgctttcg
//
LOCUS       DAZSBF010000548          730 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.556, whole genome shotgun sequence.
ACCESSION   DAZSBF010000548 DAZSBF010000000
VERSION     DAZSBF010000548.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.556"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(162..>730)
                     /locus_tag="ACHXWD_004664"
     CDS             complement(162..>730)
                     /locus_tag="ACHXWD_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR7623289.1"
                     /translation="FPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVI
                     AGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFI
                     AVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQI
                     HLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          225 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga
//
LOCUS       DAZSBF010000549          721 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.557, whole genome shotgun sequence.
ACCESSION   DAZSBF010000549 DAZSBF010000000
VERSION     DAZSBF010000549.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.557"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>721
                     /locus_tag="ACHXWD_004665"
     CDS             <1..>721
                     /locus_tag="ACHXWD_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR7623290.1"
                     /translation="FADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSL
                     KPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNIKKWHEANLT
                     PYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQ
                     QTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQY
                     KHLFAHGEYIKGITSLIKYKLK"
BASE COUNT          224 a          139 c          144 g          214 t
ORIGIN      
        1 ctttgctgat ctgccgacgt cacagttttg gtcgtacgcg acatacttca gggtattgtc
       61 ctttgaatat ctgagtgaaa gtatttccac actgctgtat ctggatgccg atgtcgtttg
      121 taaaggaagt ctgaagcctc tcacagaaat tatatttaaa gatgagtttg ctgcggtcat
      181 tcctgacaat gttagtactc aggcggcatg tgcaaaacgc ctcaacattc ccgagatgaa
      241 tggacgttat ttcaatgcag gcgttatcta tgtcaatatt aaaaaatggc atgaagcaaa
      301 tttgacaccg tatttactca cgcttttacg aggggaaact aaatatggct ctcttaaata
      361 tttagatcag gatgcgttga atatcgcatt taatatgaat aatatctacc tcgcgaagga
      421 ttttgatact atttataccc tgaaaaacga acttcatgat cgtagtcatc gaaagtatca
      481 gcaaaccatt accgataaaa cagtattgat tcactataca gggataacta aaccatggca
      541 tagctgggct ggttatccgt ctgcatcata ctttaatatc gcgcgtgaac aatctccctg
      601 gaagaaatat cctcttaaag aggcgcggac tgttgcagaa atgcagaaac aatataagca
      661 tctgtttgcc catggtgagt atattaaagg cataacttca ttaattaagt acaagcttaa
      721 g
//
LOCUS       DAZSBF010000550          720 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.558, whole genome shotgun sequence.
ACCESSION   DAZSBF010000550 DAZSBF010000000
VERSION     DAZSBF010000550.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.558"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            35..526
                     /locus_tag="ACHXWD_004666"
     CDS             35..526
                     /locus_tag="ACHXWD_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR7623291.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          269 a          113 c          116 g          222 t
ORIGIN      
        1 tttagcatca cgagacacat cacagaggaa tattatgagc acagaaacaa ttgaaatatt
       61 caataatagt gatgaatggg caaatcaact aaaacacgca ttatcgaaag gagaaaatct
      121 ggcattacta catggtttaa ctcctgatat ccttgataga atatatgcat atgcattcga
      181 ctaccatgaa aagggtaata taacggacgc agaaatttat tataaatttt tgtgcattta
      241 tgcgttcgaa aatcatgagt atctaaaaga ttttgcatca gtatgtcagc caaaaaagaa
      301 atatcaacaa gcatatgacc tttacaaact aagttacaat tactccccgt atgatgacta
      361 ttcagttatt tatcgtatgg gtcaatgtca gattggggct aaaaatatcg ataacgcaat
      421 gcaatgtttc tatcacatta ttaacaattg tgaggatgat agtgttaaga gtaaagcgca
      481 ggcatatatt gaactcttaa acgataattc agaagataat ggctaagcat tacaatcatt
      541 tggttaaatt tgctggttat agtgcagtta actactataa ccagcacctc ttatgttttc
      601 ttaaatcagc atcttaaaac agttaagagc atactataaa tcattatttt tcaatgcgtt
      661 agagaacaat ctactttata aaaattgaat aaggctattt cagattacat cactgaattc
//
LOCUS       DAZSBF010000551          711 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.559, whole genome shotgun sequence.
ACCESSION   DAZSBF010000551 DAZSBF010000000
VERSION     DAZSBF010000551.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.559"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..710
                     /locus_tag="ACHXWD_004667"
     rRNA            <1..710
                     /locus_tag="ACHXWD_004667"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          161 a          156 c          238 g          156 t
ORIGIN      
        1 tgtttgatgt tctaacgtgg acccgtgatc cgggttgcgg acagtgtctg gtgggtagtt
       61 tgactggggc ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg
      121 tcggacatca ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc
      181 gagcaggtgc gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc
      241 aacggataaa aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg
      301 gcggtgtttg gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg
      361 gtatggctgt tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt
      421 tcggtcccta tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg
      481 accggagtgg acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct
      541 aaatgcggaa gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt
      601 ctccctgacc ctttaagggt cctgaaggaa cgttgaagac gacgacgttg ataggccggg
      661 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat gaaccgtgag g
//
LOCUS       DAZSBF010000552          710 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.560, whole genome shotgun sequence.
ACCESSION   DAZSBF010000552 DAZSBF010000000
VERSION     DAZSBF010000552.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.560"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          245 a          109 c          147 g          209 t
ORIGIN      
        1 gatcgattca gatgccattt tgcagtagtc tattttataa aaaaattaag taggctaaga
       61 agtaccttag aaatcaattt taaggggtct aaaacgctct gtaacgagct aaactgtata
      121 agtggcagta agatgccttt tagactgaca ctcgcttaga gagcttccta gagcttctgg
      181 gaggattcat tagatatcgt tgtatacaga gacacagtat ctataaaggg tattaagaga
      241 ttcttagaag ttactgtaaa gaaccactaa gataagaaga gagggacaaa gaaccgaagg
      301 aaagtccctt attaagtagg agtcttaata gactgtctta atagataact taaaagtctt
      361 aaaggatatc ttccaagaag agaggaacga agaaccagag gaagtgaccg ttgaccgttt
      421 gaacagactg tttaagtaaa gcctataaag tatctattaa ttaagggcta tgtctcaata
      481 ctagcagttt gtcaatacct ttgtctttag caattcgtgc tatttattaa atatagcact
      541 ttttgttaaa acagagtatg ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca
      601 tgttcttggg ttcttagtga gagttacact atcttctaag gtactcaata aaaagacgag
      661 taacaaccga agaacgagga tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZSBF010000553          707 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.561, whole genome shotgun sequence.
ACCESSION   DAZSBF010000553 DAZSBF010000000
VERSION     DAZSBF010000553.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.561"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>707
                     /locus_tag="ACHXWD_004668"
     rRNA            <1..>707
                     /locus_tag="ACHXWD_004668"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          193 a          158 c          211 g          145 t
ORIGIN      
        1 gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct ggttttccag
       61 gcaaatccgg aaaatcaagg ctgaggcgtg atgacgaggc actacggtgc tgaagcaaca
      121 aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac
      181 cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact
      241 aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtcccttg
      301 cggatggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca
      361 gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt
      421 taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt
      481 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat
      541 ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa
      601 gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac
      661 tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZSBF010000554          698 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.562, whole genome shotgun sequence.
ACCESSION   DAZSBF010000554 DAZSBF010000000
VERSION     DAZSBF010000554.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.562"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>698
                     /gene="icsB"
                     /locus_tag="ACHXWD_004669"
     CDS             <1..>698
                     /gene="icsB"
                     /locus_tag="ACHXWD_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFR7623292.1"
                     /translation="VYIPLSGDNKTKDGKISYNLFGLDETNMSKFICQKKADAFRQLA
                     NYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATPNNVYAYANKVRQRIESLNQ
                     SYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPKTFKPLSISLYKHPTENVSS
                     EDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEEIIEKSIKTIDYYNSLKSPD
                     LGTKLYIHDLLQVN"
BASE COUNT          265 a          131 c           98 g          204 t
ORIGIN      
        1 agtatacatt ccattatcag gtgataataa aacaaaggat ggtaaaatca gctataacct
       61 ttttggcctt gatgaaacaa acatgtcaaa atttatatgc caaaagaaag cagatgcttt
      121 cagacaattg gcaaattata aattaatcag taaatctgaa aactgtgctg gtatggcact
      181 taatgttctt aaagcaggaa attctgaaat atactttcca ctccctgacg ttaaattggt
      241 tgctacacct aacaatgtat acgcgtatgc aaataaagtt aggcaacgca ttgaaagtct
      301 caatcagtct tataacgaga taatgaagta tattgaatct gattttgatc tttccagatt
      361 gactcaatta cgacgtagct atcttaaaag tttcaataaa attaacctta tccacacccc
      421 aaaaacattc aagcctttat caatttcact ttacaaacat cctactgaaa atgtatcttc
      481 agaagacttt gatgcggtca tcaacgcttg ccactcatac ttagtaaaat cagcacccag
      541 caatatgtct cgtgtattaa acgaattaaa aaccggagca acagataaaa aagaagaaat
      601 tatcgaaaag tcaataaaaa ctattgatta ttacaattct cttaaatcgc cagatcttgg
      661 aaccaaactt tacattcacg acttattaca ggtcaata
//
LOCUS       DAZSBF010000555          696 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.563, whole genome shotgun sequence.
ACCESSION   DAZSBF010000555 DAZSBF010000000
VERSION     DAZSBF010000555.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.563"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            324..>696
                     /locus_tag="ACHXWD_004670"
     CDS             324..>696
                     /locus_tag="ACHXWD_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFR7623293.1"
                     /translation="MSKASEPQQTPYLIPQGVYPTYMPKAEDEIDLFELLGTLWKKKW
                     VILCVTLLTTGLAAVYAFTAKEQWTAKTYIQAPRIAELGSYLKFHQAYARILNQPLDT
                     NALANGLFSDLILIAESPDTKV"
BASE COUNT          185 a          143 c          148 g          220 t
ORIGIN      
        1 tcacagaatt tacaaacata ctgttatcat tttgaaggca gatttggtct tatacaggca
       61 ttgctttata atctgcactc caaattctgc gggctatccg ccggtttgca gcagggaagt
      121 gtgggactgt atatgtctct tcacacggag tgttctcgat tatgtcctct aatcccagat
      181 atcacttgtt gtatcgcagt tggctatatc ctgtttctgc gcagcgcttt gggagctgaa
      241 actcaagggc ggtagcgtac ttttttgtca ggcttattct tcatttttat ttttaaccca
      301 ttgataaata atggattggt ttcatgtcaa aagcatctga accacaacag accccttatc
      361 tgatcccgca aggggtctat ccaacttata tgccaaaagc agaggatgaa atcgatcttt
      421 tcgagctttt aggcaccttg tggaagaaaa aatgggttat tttatgtgtc acgttgctga
      481 ctacggggtt agcagcagtg tatgccttta ccgcaaaaga gcagtggacg gctaaaacct
      541 atattcaagc accacgtatt gctgaattag gcagctatct taaatttcac caagcgtatg
      601 cccgaatatt aaatcaaccg ttagatacga atgcgttggc taatggattg ttttccgatt
      661 tgattttgat tgctgaatcg ccagacacca aagtta
//
LOCUS       DAZSBF010000556          695 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.564, whole genome shotgun sequence.
ACCESSION   DAZSBF010000556 DAZSBF010000000
VERSION     DAZSBF010000556.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.564"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHXWD_004671"
     CDS             <1..>695
                     /locus_tag="ACHXWD_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR7623294.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZSBF010000557          685 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.565, whole genome shotgun sequence.
ACCESSION   DAZSBF010000557 DAZSBF010000000
VERSION     DAZSBF010000557.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.565"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            127..669
                     /locus_tag="ACHXWD_004672"
     CDS             127..669
                     /locus_tag="ACHXWD_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7623295.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT          219 a          112 c          141 g          213 t
ORIGIN      
        1 gttaatttgt ttcaaataaa taaaacaatg tgtttacatt gcggtaactt ttttgatgtg
       61 agaaatttgt ttccaagaga tacagactct taacaaccaa aaaaaaattt tattaaggaa
      121 catattatga aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct
      181 ggagtatttg ctgcagacgt tgataccgga acattaacta ttaaggggaa tattgcagaa
      241 tctccgtgta aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca
      301 accactgtct ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc
      361 agcagcatgt taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatt
      421 accaacgata aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtgggt
      481 tactatctct atttaggtga taacagcgat gtcctggatg tttctgcacc ttttaacatt
      541 gagagttata aaacagcgga aggtcaatat gctattccgt ttaaagcaaa atacctgaaa
      601 ctgacagata actcagtgca atcaggtgat gtgttatctt ctctggttat gcgtgtggcg
      661 caggattaat aaataaaatt gtaag
//
LOCUS       DAZSBF010000558          684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.566, whole genome shotgun sequence.
ACCESSION   DAZSBF010000558 DAZSBF010000000
VERSION     DAZSBF010000558.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.566"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHXWD_004673"
     CDS             <1..560
                     /locus_tag="ACHXWD_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR7623296.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHXWD_004674"
     CDS             complement(616..>684)
                     /locus_tag="ACHXWD_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623297.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZSBF010000559          684 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.567, whole genome shotgun sequence.
ACCESSION   DAZSBF010000559 DAZSBF010000000
VERSION     DAZSBF010000559.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.567"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>684
                     /locus_tag="ACHXWD_004675"
     CDS             <1..>684
                     /locus_tag="ACHXWD_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7623298.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCR"
BASE COUNT          135 a          197 c          196 g          156 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgt
//
LOCUS       DAZSBF010000560          682 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.568, whole genome shotgun sequence.
ACCESSION   DAZSBF010000560 DAZSBF010000000
VERSION     DAZSBF010000560.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.568"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..426)
                     /locus_tag="ACHXWD_004676"
     CDS             complement(<1..426)
                     /locus_tag="ACHXWD_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623299.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVK"
BASE COUNT          215 a          133 c          126 g          208 t
ORIGIN      
        1 tttgactgac tgagtaaaaa attttgactc agtgggtaaa gaattttgac tcagtgggtc
       61 atctttcttg actgactgag taactttaga ctccagcttt gcctttctgg cttccttagc
      121 tgtaagctct gttaaaacct ccggtacttc atcaagtgaa taaactttaa taacacactg
      181 attgcttgca acgttagttt tcttgattaa accatgtgcc tcaagttgtt tcatacatgc
      241 aatcacggat gttcttgtga ttccagcata ctgaccaaga tgagcatatg aaggggtata
      301 atattgcccc ttatcgtgat aaaacttttg ttcattcaac atgacagtgt aaataatacg
      361 gtcggcagct ttaacgccat tagtcatctg ataaatacgg tcatcaaaca tgtggaattt
      421 ttccattata ttttcctttt taaaaataaa acaaagaaag ggctgaccac gtatagtgac
      481 caaccccaga gggagtgaat taattagaag atggactgat aaaggaactc agaagcctga
      541 gcgattacgc gagtattaac agcaaaagga aaagccttga ttgaataaga ggccgtgaaa
      601 atctgtcaat accctgacgc ctcccaacgt tgtaacctct taatgttctg agttctttta
      661 tcaccccata taaaaactat aa
//
LOCUS       DAZSBF010000561          677 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.569, whole genome shotgun sequence.
ACCESSION   DAZSBF010000561 DAZSBF010000000
VERSION     DAZSBF010000561.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.569"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>677
                     /locus_tag="ACHXWD_004677"
     CDS             <1..>677
                     /locus_tag="ACHXWD_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR7623300.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQ"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAZSBF010000562          670 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.570, whole genome shotgun sequence.
ACCESSION   DAZSBF010000562 DAZSBF010000000
VERSION     DAZSBF010000562.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.570"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHXWD_004678"
     CDS             <1..82
                     /locus_tag="ACHXWD_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR7623301.1"
                     /translation="CDYNRWRRHRWCGGLSPEQFENQNLA"
BASE COUNT          209 a          132 c          126 g          203 t
ORIGIN      
        1 gtgcgattac aatcgctggc gacggcaccg ttggtgtggc ggcctcagtc cggaacaatt
       61 tgaaaaccag aacctcgctt aggcctgtgt ccatattacg tgggtaggct cacagagcct
      121 cacttacgac tgcgtccata ttatgtgggt aggatcaata aagacgtttt atatgtgtct
      181 gcttgcagcc agaaaattta taccctgcag gtatcataaa catgcttgat aacttaattg
      241 gggctccctc ttccttttat ggtgtaattg tttgattgaa tacttccggg gatttcagta
      301 tgaaatgaag tatatttaat atactttaca aaaagtttgt attagtggaa taaggacaat
      361 agcttatcca aactgccaag cctctttatt ataagtaaga tctggcactg gaatattctc
      421 aaaaggacta acatcatcac cacgaccttc aaccaaccat tttattgcat ttaaatccca
      481 gaatagttca gtagtggtca atttgggaaa cgcacaatat tgtacggtta cgttatcgca
      541 atagagtgaa ctcccgccct atacggcgtt ttcaaggatt agtccttaat cggacaacca
      601 cattaaagca taataaataa ttaagcttta ataatatact atagatacag ttctattata
      661 aaaaatacta
//
LOCUS       DAZSBF010000563          669 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.571, whole genome shotgun sequence.
ACCESSION   DAZSBF010000563 DAZSBF010000000
VERSION     DAZSBF010000563.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.571"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(19..378)
                     /gene="traA"
                     /locus_tag="ACHXWD_004679"
     CDS             complement(19..378)
                     /gene="traA"
                     /locus_tag="ACHXWD_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR7623302.1"
                     /translation="MNTVLSVQGASAPVENKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(412..639)
                     /gene="traY"
                     /locus_tag="ACHXWD_004680"
     CDS             complement(412..639)
                     /gene="traY"
                     /locus_tag="ACHXWD_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFR7623303.1"
                     /translation="MIKGALRSAPSRKVLLTLDEETNKLLLGARERSGRTKTNEALVR
                     LQDHLQRYPDFYNEEIFREVIEENESTFKEL"
BASE COUNT          204 a          171 c          111 g          183 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa taaatacaga
       61 gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
      121 gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca tagctaactc
      241 agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc gaagcatatt
      301 cagacgagtg aatttagaaa aaaacgattt attttcaacg ggcgcagaag caccctgaac
      361 acttaaaaca gtattcatat gattcctctg attaatattg acagatgaaa actaaagttc
      421 tttaaatgtt gactcgttct cttcgatcac ctccctgaat atttcttcat tataaaaatc
      481 tggataacgt tgcaaatgat cctgcaacct caccaatgct tcgtttgttt tcgttcttcc
      541 actacgctct ctggctccaa gtaataattt attcgtttcc tcatcaaggg taagtaaaac
      601 ttttctactt ggcgcagatc ttaaagctcc tttaatcacc ctccctccca taaataaaag
      661 agatttagt
//
LOCUS       DAZSBF010000564          668 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.572, whole genome shotgun sequence.
ACCESSION   DAZSBF010000564 DAZSBF010000000
VERSION     DAZSBF010000564.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.572"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..382
                     /locus_tag="ACHXWD_004681"
                     /pseudo
     CDS             1..382
                     /locus_tag="ACHXWD_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            569..668
                     /locus_tag="ACHXWD_004682"
                     /pseudo
     CDS             569..668
                     /locus_tag="ACHXWD_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          186 a          119 c          215 g          148 t
ORIGIN      
        1 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
       61 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      121 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      181 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      241 tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg tgcggaggtc agatgcggtt
      301 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      361 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      421 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      481 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      541 aatcttttgg ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat
      601 actgacgtca aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc
      661 aacagcga
//
LOCUS       DAZSBF010000565          667 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.573, whole genome shotgun sequence.
ACCESSION   DAZSBF010000565 DAZSBF010000000
VERSION     DAZSBF010000565.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.573"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHXWD_004683"
     CDS             complement(<1..565)
                     /gene="ypdA"
                     /locus_tag="ACHXWD_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR7623304.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT          196 a          166 c          176 g          129 t
ORIGIN      
        1 gcacaataaa gccgatacag acgctaccga ggatcattgg aatgccgatt ttagagacga
       61 tatcgatccc caacgcggta gttggtgccc agacaattac cagaatcatg gtcaacgtct
      121 cacacaacat gccgccgaga atactgacgc gccagcgctg tgctttgggg attttcaggt
      181 ttatccagcc tgatatacaa cccgccagaa tgctggtgat aaagcagggg atcgccgtca
      241 cgccgccgat atcaattaaa taccggtgaa tacccgcaat cacgccggta atgatgccta
      301 cccacgggcc aaacagaatc ccgtcggaca tcaccgcgat aatacgtacg ttcaccagcg
      361 agccttctac gggaacgccg gaccaggtgc tgaacagggc gaacagcgag aaaatggcgg
      421 taacggcgag caattctttt ggggagtgcg ccgacttgtg caacagttcg cgaaacaggc
      481 ggatacggat gagaaagaac aggcagataa gcattaacgc tgcccgatcg aagaccgcca
      541 gcagcatgtt gaatatttcg tgcacgggtg actctgaata aggattaaac ctctatgata
      601 ggtaacctga aggctgatga ccagcaggcc gtttttgagg aaaaaccata aatttaaaag
      661 gggattg
//
LOCUS       DAZSBF010000566          666 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.574, whole genome shotgun sequence.
ACCESSION   DAZSBF010000566 DAZSBF010000000
VERSION     DAZSBF010000566.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.574"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          210 a          159 c          131 g          166 t
ORIGIN      
        1 gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat ggcctgaatc
       61 acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc
      121 attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga
      181 gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc
      241 ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa
      301 aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc
      361 cagctcacac ataccgagcg ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt
      421 tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa
      481 tgatgaatca tcagtcccct gccagaggtt cactacgtaa cctgaccgac aatgcaccat
      541 tctggtgctg aaaaaagagc actggaactg caacaaaaca acatcacaga acaggagatg
      601 gtaaggagtc agacattccc acacaatatt gtgtcagtgc aaataacaac cttcgtctca
      661 aatctc
//
LOCUS       DAZSBF010000567          657 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.575, whole genome shotgun sequence.
ACCESSION   DAZSBF010000567 DAZSBF010000000
VERSION     DAZSBF010000567.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.575"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            19..>657
                     /gene="virA"
                     /locus_tag="ACHXWD_004684"
     CDS             19..>657
                     /gene="virA"
                     /locus_tag="ACHXWD_004684"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFR7623305.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASNSNSTKIYFNNIEIPLKLIA"
BASE COUNT          256 a          125 c           96 g          180 t
ORIGIN      
        1 tacatcagga gaaatcaaat gcagacatca aacataacta accatgaaag aaatgactca
       61 tcatggatgt caactgtcaa atcaactaca gaagtaagtt ggaataaatt gagtttttgt
      121 gacgttttac tgaaaataat aactttcggc atatattctc cacacgaaac tctagccgaa
      181 aaatatagcg aaaaaaaact aatggatagt ttctcaccat cactatcaca agataaaatg
      241 gacggagagt ttgcccacgc aaacatagat ggcatttcaa tacggctatg ccttaacaaa
      301 ggcatatgtt cggtttttta tcttgatgga gataaaatac aatccactca actttcatca
      361 aaagaatata ataacttgtt aagttcactc ccccccaaac aattcaactt aggaaaagta
      421 catactatta cagctccggt atcagggaat tttaaaactc acaagcctgc accagaagtt
      481 attgaaacag ccattaattg ctgtacatcg ataataccaa acgatgatta cttccccgtt
      541 aaagacactg attttaatag tgtttggcat gatatttaca gagatattag agcatctaac
      601 agcaatagta caaaaatata tttcaacaat attgaaatac cactaaaatt aattgca
//
LOCUS       DAZSBF010000568          641 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.576, whole genome shotgun sequence.
ACCESSION   DAZSBF010000568 DAZSBF010000000
VERSION     DAZSBF010000568.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.576"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHXWD_004685"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHXWD_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR7623306.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZSBF010000569          639 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.577, whole genome shotgun sequence.
ACCESSION   DAZSBF010000569 DAZSBF010000000
VERSION     DAZSBF010000569.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.577"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHXWD_004686"
                     /pseudo
     CDS             complement(19..369)
                     /gene="traA"
                     /locus_tag="ACHXWD_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(415..>639)
                     /locus_tag="ACHXWD_004687"
     CDS             complement(415..>639)
                     /locus_tag="ACHXWD_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR7623307.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          164 a          173 c          137 g          165 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaatccggc aacagacata cctactgcaa taaatacaga
       61 gagtatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgactgcacc
      121 gaccagaact tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgtt tcctgacctt tagccatcag gtccgtgcct ttaccagcag ccatagctaa
      241 ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca ggcgaagcat
      301 attcagacga gtgaatttag aaaaaaacga tttctttttg acgggcgcag aagtaccctg
      361 aacacttaac gctgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
      421 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
      481 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggc
      541 cagctccact tctgtccggt ttttccagct cttacggtgt attacctccg ctttgtaaag
      601 gcccttgatg atctctgcca tcgcgttgtc atacgagtc
//
LOCUS       DAZSBF010000570          634 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.578, whole genome shotgun sequence.
ACCESSION   DAZSBF010000570 DAZSBF010000000
VERSION     DAZSBF010000570.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.578"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>634)
                     /gene="ipaH3"
                     /locus_tag="ACHXWD_004688"
     CDS             complement(<1..>634)
                     /gene="ipaH3"
                     /locus_tag="ACHXWD_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR7623308.1"
                     /translation="RNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQ
                     NALISLPELPASLEYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINA
                     DNNQLTMLPELPTSLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHS
                     EETEIFFRCRENRITYIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          165 a          107 c          174 g          188 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtatgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgat
//
LOCUS       DAZSBF010000571          624 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.579, whole genome shotgun sequence.
ACCESSION   DAZSBF010000571 DAZSBF010000000
VERSION     DAZSBF010000571.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.579"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHXWD_004689"
     CDS             complement(<1..140)
                     /locus_tag="ACHXWD_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR7623309.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHXWD_004690"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHXWD_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR7623310.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT          221 a          136 c          109 g          158 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaagaat gccattaaca
      601 aatgattttc agaataaatt cata
//
LOCUS       DAZSBF010000572          617 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.581, whole genome shotgun sequence.
ACCESSION   DAZSBF010000572 DAZSBF010000000
VERSION     DAZSBF010000572.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.581"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(269..>617)
                     /locus_tag="ACHXWD_004691"
     CDS             complement(269..>617)
                     /locus_tag="ACHXWD_004691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623311.1"
                     /translation="IPSLMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLL
                     NHYTESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNP
                     FNIINVICAKEHI"
BASE COUNT          193 a          128 c          109 g          187 t
ORIGIN      
        1 gcctggcata gcaagccctt ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct
       61 gtttagaaat gccaaattgt tgatctaaca aataaacctg tataaacaga acaccattta
      121 atctattccc ctgtgtaacc caccacatca cacaataaaa acaaaaaact ttaattatat
      181 ttcatagcac acaaaagtca atagaagccc tttcgtgctc ctacgtaaaa aatccatcac
      241 ttttccactg atacatcaat tcaaggcgtt atatgtgttc cttagcacat ataacattta
      301 ttatgttaaa tggattttgg cctatatttt gacctgcacc cgcataaaca ctggtttcat
      361 cgcttacagc ccttgtgaga cgtggtgctg tttctagcat ctgatccgac ataaaaccga
      421 tcattctcgt gtttagtgaa agtttagtga gagacattac agaaaggctt tctgtgtaat
      481 gatttaatag gtaaaaacga tgttgattac gcaagaaaat tccttgctga tatttgggga
      541 ttctggattt tccagatcgg ggggcgctga aaatcgcgtt aagcacctct cgcaatctag
      601 ccataaggga agggata
//
LOCUS       DAZSBF010000573          612 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.582, whole genome shotgun sequence.
ACCESSION   DAZSBF010000573 DAZSBF010000000
VERSION     DAZSBF010000573.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.582"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>612
                     /gene="istA"
                     /locus_tag="ACHXWD_004692"
     CDS             <1..>612
                     /gene="istA"
                     /locus_tag="ACHXWD_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR7623312.1"
                     /translation="CIGLTLHISASTVFEVLARFKASSLSWPLPADISHDTLEKLIFP
                     PKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQAGDKAMGYSHFCRCYRKWKK
                     TRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVAIFVAVMGASNYTYVEACEG
                     QDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRYEPVI"
BASE COUNT          146 a          166 c          157 g          143 t
ORIGIN      
        1 gttgtatcgg actgacgtta cacatcagcg cctcaaccgt ttttgaggtt cttgcccgct
       61 tcaaagcctc ttctctgtca tggccattac ctgcggatat atcccatgac acgctggaga
      121 aactcatttt cccgcccaaa gacacctctg cgtcagagct tgttatgcct gacatgctgt
      181 acttcgatac ggaaatgaga aaacctggcg tgacgcgcca actgttgtgg atggaataca
      241 aggcacaggc gggtgacaaa gcgatgggat actcacactt ctgccgttgc taccggaagt
      301 ggaaaaaaac gcgacgcctc tccatgcgac aggagcatcg tgctggcgaa aagctcttta
      361 tcgacttctg tggtcctacg gtccctgtta ttaatccgga caccggagag atacgccgcg
      421 tggcaatctt cgtggctgta atgggggcgt ccaactatac ctatgttgaa gcgtgcgaag
      481 gtcaggacat gatgtcatgg ttaaatgccc acagccgttg cctgaccttc ctgggcggcg
      541 taccgaagct gctcatcccc gataacctca gaagcgcagt gaaaaaagcc gatcgctatg
      601 agcctgtcat ca
//
LOCUS       DAZSBF010000574          612 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.583, whole genome shotgun sequence.
ACCESSION   DAZSBF010000574 DAZSBF010000000
VERSION     DAZSBF010000574.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.583"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..612
                     /locus_tag="ACHXWD_004693"
                     /pseudo
     CDS             1..612
                     /locus_tag="ACHXWD_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          147 a          158 c          172 g          135 t
ORIGIN      
        1 tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc
       61 ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg
      121 ggcaactgca cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc
      181 aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa
      241 atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg
      301 tatgacagtc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
      361 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
      421 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
      481 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
      541 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
      601 ataaaacact at
//
LOCUS       DAZSBF010000575          607 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.584, whole genome shotgun sequence.
ACCESSION   DAZSBF010000575 DAZSBF010000000
VERSION     DAZSBF010000575.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.584"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            61..>607
                     /locus_tag="ACHXWD_004694"
     CDS             61..>607
                     /locus_tag="ACHXWD_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7623313.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          225 a          128 c           84 g          170 t
ORIGIN      
        1 ctagcatcgc tggatgtctc ccggcaagaa tctctgattg agttaagtgc ccatcacaat
       61 atgatcactg accttatatt acacaatcat cccaggatga aaacaatcac tttaaacgac
      121 aaccatattg cacatttaaa cgccaaaaac actacaaaac tggaatattt aaacttaagc
      181 aataacaatt tactgccaac caatgacatt gatcaactaa tatcatcaaa acatctttgg
      241 catgtattag ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca
      301 gtaagaaata taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta
      361 accactcaac caccagggct gcaaaacttc acatctatca atcttgataa taaccaactc
      421 acacattttg atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatgct
      481 cttgagtcaa taaattttcc tcaaggcaga aatgtaagta ttacacatat atctatgaat
      541 aataatgctc tcagaaatat tgatatagat aggctttcat cagttactta ttttagtgcg
      601 gcacata
//
LOCUS       DAZSBF010000576          603 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.585, whole genome shotgun sequence.
ACCESSION   DAZSBF010000576 DAZSBF010000000
VERSION     DAZSBF010000576.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.585"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>603)
                     /locus_tag="ACHXWD_004695"
     CDS             complement(<1..>603)
                     /locus_tag="ACHXWD_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR7623314.1"
                     /translation="PSIRDSDDEELVANILGYIFLDDKPTSGSTSLDTFYGEGSTSHA
                     IHTRTQLENYIQTNGADKIVNNYLFVYEMIQKLFDANNLNFRSHILGNASSSQECPRY
                     YQAVFLALYELIINENMQLDDEQKFIAQLGDSVQRSMVQTEGGRWAASARQKSVEDLC
                     ALIRRYFKESENKFINHAWQTLIRTLLNNSRTEQPNYDFK"
BASE COUNT          177 a          109 c          105 g          212 t
ORIGIN      
        1 tgtttaaaat cataatttgg ttgttctgtt cttgagttat taagtagagt tcttatcagt
       61 gtttgccaag catgattgat aaatttattt tctgattctt taaaatatcg tctgattaaa
      121 gcacataaat cttcgacact tttctgacga gcgctagctg cccaacggcc cccttcggtt
      181 tgcaccattg aacgttgtac actgtctcct aactgagcga taaatttctg ttcatcgtca
      241 agttgcatgt tttcatttat gattaattca tacaaagcaa gaaaaacagc ttgatagtaa
      301 cgaggacatt cttgagatga agatgcattg cccagaatgt gtgatctaaa gttcaaatta
      361 tttgcatcaa ataatttttg aatcatctca taaacgaaga ggtaattatt gacaatttta
      421 tctgcaccat ttgtctgaat gtaattctct aattgagttc gtgtatggat tgcatgactc
      481 gtcgaacctt caccgtaaaa tgtatcgagc gatgttgatc ctgatgttgg cttatcatct
      541 aaaaatatat agcccaagat atttgcaact aactcttcat cgtcagagtc tctaatgcta
      601 ggc
//
LOCUS       DAZSBF010000577          593 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.586, whole genome shotgun sequence.
ACCESSION   DAZSBF010000577 DAZSBF010000000
VERSION     DAZSBF010000577.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.586"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHXWD_004696"
     CDS             <1..91
                     /locus_tag="ACHXWD_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623315.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHXWD_004697"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHXWD_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR7623316.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZSBF010000578          587 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.587, whole genome shotgun sequence.
ACCESSION   DAZSBF010000578 DAZSBF010000000
VERSION     DAZSBF010000578.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.587"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..66)
                     /locus_tag="ACHXWD_004698"
     CDS             complement(<1..66)
                     /locus_tag="ACHXWD_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623317.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQ"
     gene            complement(66..431)
                     /locus_tag="ACHXWD_004699"
     CDS             complement(66..431)
                     /locus_tag="ACHXWD_004699"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623318.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGISADG
                     MYCPECGTVHWPDGVIPPF"
BASE COUNT          159 a          145 c          137 g          146 t
ORIGIN      
        1 ctgcttctcc agccagttaa gccatcggtt tccctgctga aaaatatcgg caaaacgggg
       61 aagcatcaga aaggcggaat aacgccatcc ggccagtgaa ccgtaccgca ttcagggcaa
      121 tacatgccgt cggcgctgat accggcagga attgtcgcaa attcccgctc cgtgcagcgg
      181 gcaatttccg gatacccttc gtcatcaaca cgtacaaacc agaagaccag ttttttgttt
      241 ccggcatcca caaagaacgg aatattcagg tctgcgcaac actcaacggc atcgtcaaaa
      301 ttatcaaagc gcagaacctc agcttcttct tcgtcaaaaa aatcatcttc gtgaagtttt
      361 acgacatagc ggaggagttt gcttctttga ggtgcaggtt ttcgtttgcg tggtttagct
      421 gaacgggcca tataactacc tgaaagacaa tgatattgtc tgtttttata acggtaatca
      481 caggccatga caagccgcag ccgtcaggcc gcctactcgg ggcggtttag aacgcttatt
      541 tagtaccaga tcttacttat aataaagggg cttggcagtt tggataa
//
LOCUS       DAZSBF010000579          585 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.588, whole genome shotgun sequence.
ACCESSION   DAZSBF010000579 DAZSBF010000000
VERSION     DAZSBF010000579.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.588"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          180 a          128 c           98 g          179 t
ORIGIN      
        1 aaaaaaaata cctcattgtg cgaataaagt aacgggggcc atagatttct ctacctacct
       61 tttggtgttc atacctgagg ctgatagttt caggattccg cgacaattct actcaccttc
      121 ttgttctctc gccaatatga aatcaatcag aaaagcgttc cataagaaca acgataagac
      181 aataaattgt catcacggct gcaatagcca acgtgccttt cagtaccaac atgacaacct
      241 cctgtatttg acgtaacgtg tgctgataaa tatgtatttg tatctcaact gactcaatac
      301 aacacttaag tatagttttt cagattttgt ttttcccgtg aaatattatg gaaatggcag
      361 aagcaataca ttgaattccg attcgagtga atacggctag cgggcacctc actttagcac
      421 tgaagccatc aacttttatt tgacttattt caatggaaag ctttctcact atcagtacag
      481 gtagttccca tccttttaaa gtcgagagtc agactgcgac taatacagtt aaagtgacca
      541 aactcagtta tcctctacac attttacctc atgtttactt ggaca
//
LOCUS       DAZSBF010000580          576 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.589, whole genome shotgun sequence.
ACCESSION   DAZSBF010000580 DAZSBF010000000
VERSION     DAZSBF010000580.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.589"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..518)
                     /locus_tag="ACHXWD_004700"
     CDS             complement(<1..518)
                     /locus_tag="ACHXWD_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR7623319.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDSK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDAKGNDASNP"
BASE COUNT          161 a          138 c          115 g          162 t
ORIGIN      
        1 catgggttgc tggcatcatt gcctttcgca tcacccggac agttagaaga ggctacccac
       61 caagcatttt tatataccgc accattaaag acgacatacc aggtctgacc gccctgctgt
      121 ttattccagg caactacaga agaattagac ggaacagtgc cctgtgctgg tgtagccgaa
      181 ccgccattag ccggcgtatt ggatgcaggc gtatttgaat ttgaagaaga tgagctgccg
      241 ctcttttcgc aggaaagtgt attgccaaac tgactaattt cagcagctgt tgcggtacgc
      301 tttaaacgcc acgggttagt tgcatcattt gctttcgctt ttcccgggca atttgtagaa
      361 aaaccccacc aggcattttc ataaattttg ctatcgaaaa taacctgata tttattacca
      421 ccttgttggt tattccatgc ttccattgcc aatgctggca gagcggaaca caccagcccc
      481 ataccaatca tagatttagt aaatatattt aatttcatta taatcccttg tgacgtaaaa
      541 actgcaaaac aaaagtccta cgcaggaatt atttac
//
LOCUS       DAZSBF010000581          575 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.590, whole genome shotgun sequence.
ACCESSION   DAZSBF010000581 DAZSBF010000000
VERSION     DAZSBF010000581.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.590"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          157 a          113 c           91 g          214 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaatatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgtttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgt
//
LOCUS       DAZSBF010000582          567 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.591, whole genome shotgun sequence.
ACCESSION   DAZSBF010000582 DAZSBF010000000
VERSION     DAZSBF010000582.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.591"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..502)
                     /gene="mxiE"
                     /locus_tag="ACHXWD_004701"
     CDS             complement(<1..502)
                     /gene="mxiE"
                     /locus_tag="ACHXWD_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFR7623320.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTIT"
BASE COUNT          185 a          117 c           79 g          186 t
ORIGIN      
        1 aagtgattgt ttgattgtgc aaaaatacat ctaataaacc atttactaac cgccaagtgt
       61 tcagttgttc tttgactttt gcccctaaag cttttctaca taaagatcta aaataagcct
      121 ccgacacacc ataatgctct gtcagactct ttattcttac ctccttttct ttctcaatag
      181 ttctcagaag ataaagaacc aaatgatatg actcagtcat tcgtatcata gccagtgcat
      241 tacttacatt ctggtcagat gaaaagatga atcttaacat taacttatca agcatcgatt
      301 cggtattaca actatcccga agatcattct catgatagaa atgaatacat ttttcagcat
      361 ggctatgctc tactagagca ccactgaaat agtcccaaaa agcaagaaat tttgcacaac
      421 tattaatgtt aaagcgataa tgatagttca cattatcact gaattttata ttttgatttc
      481 ggacaaaaaa aaacccttcc atgctggagc cagctcgatt atgttacgtt ctttatcttt
      541 tacaagctca acgttaactg gttcatg
//
LOCUS       DAZSBF010000583          557 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.592, whole genome shotgun sequence.
ACCESSION   DAZSBF010000583 DAZSBF010000000
VERSION     DAZSBF010000583.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.592"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHXWD_004702"
     CDS             <1..>557
                     /locus_tag="ACHXWD_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HFR7623321.1"
                     /translation="IPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRALNARL
                     RELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPDYHVEY
                     ARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHMPERHR
                     RHGTWTPERLIEQGNRTGPSTGRVV"
BASE COUNT          142 a          169 c          148 g           98 t
ORIGIN      
        1 tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa gcagaaaacg gcgtcctcat
       61 cgtcgagcga tggcttcttg cccgcatccg taatgaaacc ttccacacgc tcagggcgct
      121 gaatgcacgc cttcgggagt tgctgacgga catgaataac cgaccaatga agggatacgg
      181 taatcaaacc cgcgcagaac gcttccgcat gctggatgca ccagcactct ctccgctgcc
      241 tctggagcct tatgaataca cggaatacaa agccgtgaag gttggccctg actaccacgt
      301 tgaatatgcc cgtcactggt actccgttcc acacgaactg gtcggtcagc gtctgtcact
      361 gaaggtgggg cagtcggtgg tacagctctg gcacaagggg cagtgtgtgg cgcaacatcc
      421 gcgcagcaca catgaatata aacacacaac caatccgctt cacatgccag aacgccaccg
      481 gcggcatggg acatggacac cggaacgcct tatcgagcag ggaaaccgta caggtccgtc
      541 cacaggccgg gtggttg
//
LOCUS       DAZSBF010000584          555 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.593, whole genome shotgun sequence.
ACCESSION   DAZSBF010000584 DAZSBF010000000
VERSION     DAZSBF010000584.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.593"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            32..>555
                     /locus_tag="ACHXWD_004703"
     CDS             32..>555
                     /locus_tag="ACHXWD_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR7623322.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
BASE COUNT          172 a          102 c          114 g          167 t
ORIGIN      
        1 tttggaaaca atattgtaag atcaaattta tatggtggcg aaaattaatc tcttatcacg
       61 gcttacgccg cttttattcg tgttcgctcc ttttttggca cagtcaaata tgactgttta
      121 tcctatggct gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag
      181 taatgaagtt cagtacatca aggcgacagt attccgtatc gataatccat cgacgcctca
      241 ggaaaatgaa gttgagatta aatccggtga tgcaaatcat ctggttgtta tgccacctaa
      301 attcgcttta ccagccggta gtagcaaaac tgtgcgcttt gttgcaatgg aaccagagca
      361 aaaagaaaaa aattatcgcg ttaaatttga agcggttccc agtattgatg acgttgccac
      421 agataaaaaa gatctctcta tgcagttaac agttaactta atttggggga ttgttgttag
      481 tgttccacct cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa aattagttaa
      541 tgcagggaat caacg
//
LOCUS       DAZSBF010000585          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.594, whole genome shotgun sequence.
ACCESSION   DAZSBF010000585 DAZSBF010000000
VERSION     DAZSBF010000585.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.594"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(448..>550)
                     /locus_tag="ACHXWD_004704"
     CDS             complement(448..>550)
                     /locus_tag="ACHXWD_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFR7623323.1"
                     /translation="RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
BASE COUNT          169 a           87 c          100 g          194 t
ORIGIN      
        1 agtaattata tttgagaaat ctgctaatat attcttaaag cttttttgat actgtgaacg
       61 aagtctgtta atggtggatt ttttgtttat caccatgtaa aaaaattgag tagtaaaagc
      121 gttgactgct aatcctcgct gtttttattg aaacaattaa aaatacgctg aatattttcg
      181 gccagtcact catcttcaca taaaattttg accatcatgc cgcatccctt agttgacaat
      241 tcattttcaa tgatatgaat attactaatt tcaccagata atgttctcgt ggtcagcagg
      301 gtatgagttt tcaccgtaat tttaaggaaa cacagtgcgt ggggattgaa taacagatag
      361 ccgacggaat taccccaccg gctgaaagtc aagttcaacg aagattttta ttatctgaat
      421 tgggcagttt acatcagtgt tcgatgttta tgaagacgat agatggcgag aaattatatc
      481 ccgaatagct tcatctattt ctctctgaac tgttttgttt attttagata atgtaaatat
      541 tagagttctg
//
LOCUS       DAZSBF010000586          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.595, whole genome shotgun sequence.
ACCESSION   DAZSBF010000586 DAZSBF010000000
VERSION     DAZSBF010000586.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.595"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..408
                     /locus_tag="ACHXWD_004705"
     CDS             <1..408
                     /locus_tag="ACHXWD_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFR7623324.1"
                     /translation="ADIHGRVVRVLDGDTIEVMDSLKAVRIRLVNIDAPEKKQDYGRW
                     STDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTD
                     PVLPVLQNEARQQKRGLWSDADPVPPWIWMHRK"
     gene            454..>550
                     /locus_tag="ACHXWD_004706"
     CDS             454..>550
                     /locus_tag="ACHXWD_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFR7623325.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSL"
BASE COUNT          164 a          113 c          143 g          130 t
ORIGIN      
        1 gcagatattc atggacgggt tgttcgtgta ctggacggtg ataccatcga ggtcatggac
       61 tcactgaagg cggtgcggat caggctggta aatattgatg caccggaaaa gaaacaggat
      121 tatggccggt ggtcaacaga catgatgaaa tcactggtcg caggaaaaac ggttaccgtc
      181 acatattttc agcgagatcg ttatggtcgt attctcgggc aggtttatgc accagacgga
      241 atgaatatta atcagtttat ggtccgggcc ggggctgcct gggtatatga gcagtacaac
      301 acagatcccg tgctacctgt ccttcagaac gaagccagac aacaaaaacg tgggctctgg
      361 tcagatgctg atccggttcc cccctggata tggatgcacc gtaaataaag cgatggcaat
      421 cctatcattc atctgttctc agcgggaata aaaatggcaa aaacaaaaca ggaatggctg
      481 tatcagttac ggcgatgttc atcagtcaat acgctggaaa aaattattca taaaaacaga
      541 gacagtttat
//
LOCUS       DAZSBF010000587          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.596, whole genome shotgun sequence.
ACCESSION   DAZSBF010000587 DAZSBF010000000
VERSION     DAZSBF010000587.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.596"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="ACHXWD_004707"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="ACHXWD_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR7623326.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAZSBF010000588          550 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.597, whole genome shotgun sequence.
ACCESSION   DAZSBF010000588 DAZSBF010000000
VERSION     DAZSBF010000588.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.597"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..506)
                     /locus_tag="ACHXWD_004708"
     CDS             complement(<1..506)
                     /locus_tag="ACHXWD_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR7623327.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTII"
BASE COUNT          167 a          108 c          120 g          155 t
ORIGIN      
        1 ttaatgatgg ttaacggtaa tagcgtgtca ccacagcaaa ccgtctgtgt aatttcgtgc
       61 ttacgtggtt cttttttaaa aacaacgact gcactaggaa acggtgaacg aatagtaagc
      121 ccgttatggc ctgctggtgg aaggtattta actgcacgat gaagaatacg gatttcatga
      181 cagtggttat tgctgtaaat gtgcctaagc cagtagctgg tgttggctct atgcggtatc
      241 aggaaagcta ctaaatccgc ttcagacgct tttaagagga acgacggaat atcgctgtac
      301 ggcaggttac agaagacctt ttcactaacc catgattgac ggcttatatc attggtgaat
      361 cgtggtaaca gcgtgtcatg ctcagttgca gcaccatcta gcgtaaaacc aaattcacgg
      421 tgtaagttct taaataacaa caatggagtt ctccagtaat cagaaccccc atgatgtttc
      481 ccgattaaaa tatgctcaat tgacataaga aataaaaaat tttattgcaa tcactcttga
      541 caaggtttac
//
LOCUS       DAZSBF010000589          549 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.598, whole genome shotgun sequence.
ACCESSION   DAZSBF010000589 DAZSBF010000000
VERSION     DAZSBF010000589.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.598"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>549
                     /locus_tag="ACHXWD_004709"
     CDS             <1..>549
                     /locus_tag="ACHXWD_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR7623328.1"
                     /translation="VVISKYTDHLPLYRQQHIFSRMGVELPVSTMADMVGVAGAALAP
                     LVKLLRHELLTRDVIHADETSLRLLDTRKGGKSCSGWLCAYVSGERSGPPVVCFDSQT
                     GRALRYPETWLQCWCGGTLVSDGYSVYKSLADNHPGITSACCWSHAGRGFANLYKASR
                     EPRAGVELRKIAGLYRIEKLIRE"
BASE COUNT          113 a          150 c          166 g          120 t
ORIGIN      
        1 gttgtcatca gcaaatatac cgaccatctg cccctgtatc gccagcagca cattttctcc
       61 cgaatgggtg tggagctccc cgtcagtaca atggcggata tggtcggtgt tgccggagct
      121 gcactggccc cgctggtgaa actgctacgc catgagcttc tgacacggga tgttattcat
      181 gcagatgaaa cgtccctgag gctccttgat acccggaaag gcggtaaatc ctgctccggc
      241 tggctgtgtg cttacgtcag cggtgaaaga agcggtccgc cggtcgtctg ttttgactcg
      301 cagaccggac gggccctcag ataccctgag acctggctgc agtgctggtg tggcggtacg
      361 ctggtgagtg acggatacag cgtgtacaaa tctctggcgg ataatcaccc cggcatcaca
      421 tcagcctgtt gctggagtca cgcagggcgc ggcttcgcaa atctgtataa ggccagcagg
      481 gagccccgtg caggcgtgga gctgagaaaa atcgcgggtc tttaccgcat tgagaaactc
      541 atccgtgag
//
LOCUS       DAZSBF010000590          546 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.599, whole genome shotgun sequence.
ACCESSION   DAZSBF010000590 DAZSBF010000000
VERSION     DAZSBF010000590.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.599"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            97..>546
                     /gene="ygeH"
                     /locus_tag="ACHXWD_004710"
     CDS             97..>546
                     /gene="ygeH"
                     /locus_tag="ACHXWD_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR7623329.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKI"
BASE COUNT          191 a           86 c           92 g          177 t
ORIGIN      
        1 tttcctgtag cattaatgac acataaatat gaatagccat aatttctatt gcttaagcag
       61 gatgcaagaa accaattttt tcatagaggt taactaatgg acttagaaaa taaattctca
      121 tatcattttc ttgagggatt aacgctcacg gaagatggaa ttcttactca aggaaatgag
      181 caagtttata ttccacagaa agagttaggt gtattaatag tattacttga atccgctggt
      241 catgttgtac tgaaagatat gatcatcgaa tcagtatgga aaaatattat tgttagtgac
      301 gagtccctga caagatgtat ctattctttg cgctgcattt ttgaaaaaat tggctatgat
      361 cgttgcatag aaacaatcta ccggaaaggt tatcgtttca gcgggcaggt tttcaaaact
      421 aaaattaatg aagataatac ttcagactat tccatagcta tattcccttt cactacttca
      481 ttgaatacac tggatccatt aatacttaat caggaattag tgcaaatcat ttcaaataaa
      541 aaaatc
//
LOCUS       DAZSBF010000591          541 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.600, whole genome shotgun sequence.
ACCESSION   DAZSBF010000591 DAZSBF010000000
VERSION     DAZSBF010000591.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.600"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..541
                     /locus_tag="ACHXWD_004711"
                     /pseudo
     CDS             1..541
                     /locus_tag="ACHXWD_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          193 a          117 c           82 g          149 t
ORIGIN      
        1 ttattaaaaa tgcctgttta aaattatata atttagcaca taccgcaacc aactcccacc
       61 ttaaaccaac tggtccggat aattcagatg ttctcctgtt caaaaagcta ttctcccctt
      121 cgaaattaat gacaattatc ggtgatgaaa ttcctcttat atctgaaaaa cagtcgcttt
      181 caaaggtgct tttaaatgat gagaataatg aactgagtga tggtacaaac ttctgggata
      241 aaaatcgtca attaaccaca gatgaaatag cttgctatct tcagaagatc gccgctaatg
      301 caaaaaatac tcaagtcaat tatcctactg gtctctacgt cccctactcc accagaactc
      361 acctggaaga cgctctcaat gaaaatatta agagcgatcc atcatggccg aatgaagtcc
      421 agttattccc cataaatact ggcggacact ggatattagt ttcgctacag aaaatagtaa
      481 ataaaaaaat aataaactac aaataaaatg cgtcatattc aactcattgc gtgcactagg
      541 c
//
LOCUS       DAZSBF010000592          530 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.601, whole genome shotgun sequence.
ACCESSION   DAZSBF010000592 DAZSBF010000000
VERSION     DAZSBF010000592.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.601"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>530
                     /locus_tag="ACHXWD_004712"
     CDS             <1..>530
                     /locus_tag="ACHXWD_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR7623330.1"
                     /translation="SDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQV
                     AYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSA
                     NIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNK
                     EEERFNLFISIPFYWG"
BASE COUNT          164 a          104 c          107 g          155 t
ORIGIN      
        1 tccgataact ataatgctat aactctggga aatggttgga atacaccgct gggtgccatc
       61 tctttttacg ctgcccgatc gagtagtaag ttaaataatg ataccagaca cgaaggtacg
      121 agctatcagg tcgcctataa taaatatcta gtacaaacag ccactcgatt tagcgtggcc
      181 gcctggcgtt atgcttcgca ggattacagg acattcagcg accatcttta cgaaaatgat
      241 aaacactatc atcagagtga ttatgatgat ttttatgata ttggcagaaa aaatagcctt
      301 tctgccaata ttatgcaacc tttatccaat aatctgggaa atgtatcatt aagtgcgctt
      361 tggcggaatt actgggggcg aagtggaaat gctaaagatt accaattcag ttactccaat
      421 aactggcaac acattagtta tactttctct gccagccaat cttatgatga aaataataaa
      481 gaagaggagc gttttaatct gtttatctcc attcctttct actgggggga
//
LOCUS       DAZSBF010000593          528 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.602, whole genome shotgun sequence.
ACCESSION   DAZSBF010000593 DAZSBF010000000
VERSION     DAZSBF010000593.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.602"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            245..454
                     /gene="fumD"
                     /locus_tag="ACHXWD_004713"
     CDS             245..454
                     /gene="fumD"
                     /locus_tag="ACHXWD_004713"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR7623331.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          159 a           83 c          130 g          156 t
ORIGIN      
        1 tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcgc tgtttgcgca
       61 acgtctggtg ccgaagttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaata
      121 tatcatcgct caattgctac agttaaaata atttatcgcg ttatttgtct gattttgttt
      181 gatttttgtt taattggtta gattgatata tgctgatagt tggcatgata aataggtata
      241 aagaatggga aatcggacaa aagaggatga actgtaccga gaaatgtgca gagttgtcgg
      301 taaagttgtg ctggaaatgc gcgatctggg gcaggaacca aagcatattg ttatcgcagg
      361 cgtattacgt actgcattag ccaataaacg cattcagcgc agtgagctgg aaaagcaggc
      421 gatggaaacc gtcattaacg cactggtaaa atgaccattc aggatgaggc ggtaagcttc
      481 atcctgatat tcttgcttca gttgcggtcc agttaatcat tctaaata
//
LOCUS       DAZSBF010000594          525 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.603, whole genome shotgun sequence.
ACCESSION   DAZSBF010000594 DAZSBF010000000
VERSION     DAZSBF010000594.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.603"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>525
                     /gene="ospC1"
                     /locus_tag="ACHXWD_004714"
     CDS             <1..>525
                     /gene="ospC1"
                     /locus_tag="ACHXWD_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFR7623332.1"
                     /translation="KDMKLGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFV
                     FQPEYHIPRMVSTENFKKVKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSIAN
                     QKEDVALYILSNFEITRQDVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTK
                     FKKTNSGDCMLDNA"
BASE COUNT          189 a           74 c          101 g          161 t
ORIGIN      
        1 taaagatatg aagttagggc tcggattata cctgattgac tttattagaa aaagtgaaga
       61 ccaaagcttc aaggagtttt gctatggaaa aaatcttgcc cctgtggatc tggatagaat
      121 cataaacttt gtttttcagc cagagtacca tatacctagg atggtaagta cagaaaactt
      181 caaaaaagtt aagattagag aaatatcctt agaggaggct gttacagcat ctaattacga
      241 agaaattaac aagcaggtca ctaacaaaaa aattgctctc caggctcttt ttctttcgat
      301 tgctaatcaa aaagaggatg tcgccttata tatattatct aattttgaga taactagaca
      361 agatgttatt tccataaagc atgagttgta tgatattgag tatctactta gcgctcataa
      421 ttcaagctgt aaagtacttg agtattttat caataaggga ttggttgatg taaacacaaa
      481 gttcaaaaaa actaatagtg gggattgtat gttggataac gcaat
//
LOCUS       DAZSBF010000595          521 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.604, whole genome shotgun sequence.
ACCESSION   DAZSBF010000595 DAZSBF010000000
VERSION     DAZSBF010000595.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.604"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          190 a          101 c           83 g          147 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata aactattttt aaacagacac ttaccacaca
      481 acaaactaat taaatacaaa gaaaggcaac aatcaactta a
//
LOCUS       DAZSBF010000596          520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.605, whole genome shotgun sequence.
ACCESSION   DAZSBF010000596 DAZSBF010000000
VERSION     DAZSBF010000596.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.605"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>520
                     /locus_tag="ACHXWD_004715"
     CDS             <1..>520
                     /locus_tag="ACHXWD_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR7623333.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAZSBF010000597          520 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.606, whole genome shotgun sequence.
ACCESSION   DAZSBF010000597 DAZSBF010000000
VERSION     DAZSBF010000597.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.606"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..159
                     /locus_tag="ACHXWD_004716"
     CDS             <1..159
                     /locus_tag="ACHXWD_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7623334.1"
                     /translation="RDAYCNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIR
                     LFYQRLRN"
     gene            298..453
                     /locus_tag="ACHXWD_004717"
                     /pseudo
     CDS             298..453
                     /locus_tag="ACHXWD_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          148 a          109 c          125 g          138 t
ORIGIN      
        1 cgtgatgcgt actgcaatcg taatgtcgtt gaacggtgct ttgggcggct gaaagaatat
       61 cgccgcatcg ccacgcgtta cgacaaaacg gcgagaaatt acctggcgat ggtgaaactg
      121 ggctgcatcc gactctttta tcaacgctta cgtaattaag ggacacagcc tggcactctg
      181 cgagtaaact tgctggtaaa ctattattga cagttggttt tgttttgaga ctgtaattta
      241 aattgtgtaa ttgcctgttt ttgatatgtt cactccaaca acggagacag gtaaattatg
      301 gacgaaaaga agcttaaagc ccttgcggct gaactggcta aaggcctcaa aaccgaggcc
      361 gacctcaatc agttttcccg tatgcagacg aaattaactg tcgaaacggt gcttaatgcg
      421 gaactaactg accatctcgg gcatgaaaaa agctacatcc ggtagtaaac attatcagtg
      481 ggttatgcga tatgatttga gccgttttca gacaatcgca
//
LOCUS       DAZSBF010000598          515 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.607, whole genome shotgun sequence.
ACCESSION   DAZSBF010000598 DAZSBF010000000
VERSION     DAZSBF010000598.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.607"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          151 a          127 c          101 g          136 t
ORIGIN      
        1 gattacgacc ttttaaatgc aatatagaca taataacttc ttacaaaatc tataattaat
       61 accgtagata ttcattcatc ttaccctgtg caccaaaagc aaaaactcac tgacgcgggt
      121 ttgaaactga tggcaaccgc aaaaatgcct gatgcgctac gcttatcagg cctacgccat
      181 ctctacaata tattgaattt gcgtgctttt gtaggcagga taaggcgttc acgcagcatc
      241 cggcatgaat aaagcgcact caacaatctg aaacccgccg gaacggttta ttacgtacat
      301 caggtgaaac tgaccgataa gccgctttct tttgggtata gtgtcgtgga cagtcattca
      361 tctttctgcc cctccaaaag caaaaacccg ccgaagcggg tttttacgta aatcaggtga
      421 aactgaccga taagccgctt tcttttgggt atagcgtcgt ggacagtcat tcatctttct
      481 gcccctccaa aagcaaaaac ccgccgaagc ggttt
//
LOCUS       DAZSBF010000599          511 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.609, whole genome shotgun sequence.
ACCESSION   DAZSBF010000599 DAZSBF010000000
VERSION     DAZSBF010000599.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.609"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>511
                     /locus_tag="ACHXWD_004718"
     CDS             <1..>511
                     /locus_tag="ACHXWD_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HFR7623335.1"
                     /translation="IQETKNMLDQFENRYPDLLKEVFRHVTIQRISEVLQRLLGENIS
                     VRNLKLIMESLALWAPREKDVITLVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDA
                     IRKGIRQTSGGSFLNMDIEVSDEVMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLI
                     DNRFKSILV"
BASE COUNT          171 a           63 c          111 g          166 t
ORIGIN      
        1 gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct gatctattaa
       61 aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa agattgcttg
      121 gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg ctttgggctc
      181 ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt tctaggtata
      241 tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc ggatatattg
      301 aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg aatatggata
      361 tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa ttgagaaatg
      421 caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt aaaagactta
      481 tagataacag atttaagagt atactcgtta t
//
LOCUS       DAZSBF010000600          509 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.610, whole genome shotgun sequence.
ACCESSION   DAZSBF010000600 DAZSBF010000000
VERSION     DAZSBF010000600.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.610"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHXWD_004719"
     CDS             complement(<1..>509)
                     /gene="espL1"
                     /locus_tag="ACHXWD_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HFR7623336.1"
                     /translation="LDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALLRHPPA
                     GSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQSVYGQLNYVV
                     SVYDPNDTNVAVRGTHRAARGFLSLDKFISSAPDAQTWADRYVRNCAIAILPLLPEGV
                     PVAILAGITT"
BASE COUNT          149 a          115 c          117 g          128 t
ORIGIN      
        1 gtcgtgatac ccgctaaaat agcaactgga actccctcag gtaatagagg aagaatagca
       61 attgcacagt tgcgaacata cctatcagcc caggtctgag catcgggagc tgaactgatg
      121 aacttatcaa gcgagagaaa gccccgtgct gccctgtggg tgcctctgac ggcaacgttg
      181 gtatcgttcg ggtcgtaaac agacacaaca taatttaatt gtccatacac tgactgcttt
      241 attcgtagcc ttactgacaa agtatgattg tctatatata aaatcgcgga catatgactg
      301 acattgtttg ccatcattcg acaaaagaaa atatctaaag cgaagccaaa atccttactc
      361 gcgataacgc gactccctgc aggaggatgg cgaagtaatg ctctgaactc cgcgtcccgg
      421 actaagggga catgctgctg tatggattca acggaggagt agtgtttaat caggttgact
      481 ttgccttttc catttctcaa tgaatcaag
//
LOCUS       DAZSBF010000601          507 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.611, whole genome shotgun sequence.
ACCESSION   DAZSBF010000601 DAZSBF010000000
VERSION     DAZSBF010000601.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.611"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHXWD_004720"
     CDS             complement(<1..188)
                     /locus_tag="ACHXWD_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR7623337.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="ACHXWD_004721"
                     /pseudo
     CDS             242..445
                     /locus_tag="ACHXWD_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          126 a          136 c          117 g          128 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggattcc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAZSBF010000602          507 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.612, whole genome shotgun sequence.
ACCESSION   DAZSBF010000602 DAZSBF010000000
VERSION     DAZSBF010000602.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.612"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..429
                     /gene="istB"
                     /locus_tag="ACHXWD_004722"
     CDS             <1..429
                     /gene="istB"
                     /locus_tag="ACHXWD_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR7623338.1"
                     /translation="CGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLK
                     YLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLME
                     NPTTADAILDRLVHNSHRVVLQGESLRKNPPTVESSEKTS"
BASE COUNT          157 a          106 c          144 g          100 t
ORIGIN      
        1 tgcggtaaga gctggatagc caatgcgctg ggcgaacagg catgtcgaca gaaatacagc
       61 gtccggtact gtagaaccgg gcgattactg gaacagttgg ctcagggacg tgttgatggc
      121 agttggctga aatatctgaa acagttgcaa aaaatacagg ttctaatcct ggacgatctg
      181 gggctggaac agttaagtaa cgcccagtgt aatgaccttc tggagatcac agaggatcgt
      241 tacggacaaa gcagcacgat agtggtaagc cagttcccgg tagataaatg gcatggtctg
      301 atggagaacc caacaacagc agatgccatc ctggacagac tggtacataa ctcacacaga
      361 gtggtccttc agggagagtc cctgaggaaa aatcccccga cagtggaaag cagcgaaaaa
      421 acgagttaaa aagcggtaga aaaagttaag ttcccggttg atcgaatgga tgcgaatcgc
      481 tgatcgaact tcgatgccaa tccgtga
//
LOCUS       DAZSBF010000603          504 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.613, whole genome shotgun sequence.
ACCESSION   DAZSBF010000603 DAZSBF010000000
VERSION     DAZSBF010000603.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.613"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..194
                     /locus_tag="ACHXWD_004723"
     CDS             <1..194
                     /locus_tag="ACHXWD_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR7623339.1"
                     /translation="LKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKIV
                     VKYNNWITKDNGEFELAVH"
     gene            224..>504
                     /locus_tag="ACHXWD_004724"
     CDS             224..>504
                     /locus_tag="ACHXWD_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR7623340.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          107 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcct
//
LOCUS       DAZSBF010000604          500 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.614, whole genome shotgun sequence.
ACCESSION   DAZSBF010000604 DAZSBF010000000
VERSION     DAZSBF010000604.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.614"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..127
                     /locus_tag="ACHXWD_004725"
     CDS             <1..127
                     /locus_tag="ACHXWD_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7623341.1"
                     /translation="CYTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVENGQHQ"
     gene            241..499
                     /locus_tag="ACHXWD_004726"
                     /pseudo
     CDS             241..499
                     /locus_tag="ACHXWD_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          171 a           98 c          122 g          109 t
ORIGIN      
        1 ctgctacacg ctgaatatca aaggagagag ttaccggtta aaagagaaac gtaaagctgg
       61 agtgctgacc aaaaacacaa cgccaatcag tgatgatgaa atggtggaaa acggacagca
      121 tcagtaacga aagtatctta gcaggcatga aaatcgtccg catcccctaa acggtcaaaa
      181 tgcttctgcc gtgaaggagg gagaatgcca tctcatcact tatatctggc gaatgaaaca
      241 cagatggtcg gccttgcccc caaacacacg gcggtgcaaa atggaatggc agaaaacttc
      301 gtcaaaacga tgaaatatga ttaaatcagt gttatgttcg aacctgacgt gttaacagca
      361 gtaaagtagt ctcgcgaagg tgttcaaaca ttataatgaa tggcatcctc atagtgcgca
      421 gggttatcgc ttgctgcgga aatatctgaa gcgacgtacc agtaatgggg aaaatgatag
      481 ctggtttctg aaaatataga
//
LOCUS       DAZSBF010000605          498 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.615, whole genome shotgun sequence.
ACCESSION   DAZSBF010000605 DAZSBF010000000
VERSION     DAZSBF010000605.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.615"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>498
                     /locus_tag="ACHXWD_004727"
     CDS             <1..>498
                     /locus_tag="ACHXWD_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HFR7623342.1"
                     /translation="AGIISALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYK
                     VSGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFF
                     AHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFML
                     SLCAV"
BASE COUNT          148 a           99 c           71 g          180 t
ORIGIN      
        1 agctggaatt atatctgcat tgtattttct aataacacca aaaaaaacaa taactaacaa
       61 tcctactttg ttaattttca tctcattatg tcttttggga atcataaata tcatctggta
      121 ttcacattat aaagtttcag gctctgttta taccaatgca tatcgtggcc caatggaaac
      181 tgggaaaatt gccttgtgta gtgcttttat tttcttggtt ctttttgcta aaaatgagtt
      241 gagaacaaaa ataaaatttg ggaaactaat tctgttcgca tccctggcaa cgcagttact
      301 tttttttgcg catgccatgt ggcaacattt ctatttaaac gttgaccgtg ttgcattatc
      361 agcttcccac gctacaacag caggctacat catccttttc ccttctttac tggcatcaat
      421 tctcatttta aaatccgact ttagacataa aacaacatta tatacaatta acttcatgct
      481 tagcttatgt gctgtcat
//
LOCUS       DAZSBF010000606          497 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.616, whole genome shotgun sequence.
ACCESSION   DAZSBF010000606 DAZSBF010000000
VERSION     DAZSBF010000606.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.616"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          149 a          116 c          101 g          131 t
ORIGIN      
        1 ctgtcaagct atttttttaa tagatagcac aaaatgctaa agacacccct gtaccataga
       61 cttctatctg tcatctcgtg caggggtttt gaaggagttt aacgggggtc tgtgtggtcg
      121 ttatccttaa ggtcaagcgt ccatgtacga ccgaacagtt taaagacaac aagcactctg
      181 acgaggacgt agcgaagtag caggcctgta atcagcccaa tacctccacc agtccataca
      241 tcaggtgacc ccagctcagg agggagattt accattagtt ttccttgtaa gacgctctca
      301 atcgcattct gacacaaaaa gtacctgacc ttatgagttg acctgaagag gtcgtttaac
      361 tcgttacagg gcgattgaag aggtctgtgt gcaatgcaaa atatgcgacc aaatacatag
      421 ccacgcctca tattttactc tccccataaa cccattatac cacagatgtt acgaaaatgt
      481 taaaaatact tgacatt
//
LOCUS       DAZSBF010000607          497 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.617, whole genome shotgun sequence.
ACCESSION   DAZSBF010000607 DAZSBF010000000
VERSION     DAZSBF010000607.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.617"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            47..>497
                     /locus_tag="ACHXWD_004728"
     CDS             47..>497
                     /locus_tag="ACHXWD_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR7623343.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          119 a          119 c          130 g          129 t
ORIGIN      
        1 gggtgagctg ttgctacacc ccggtatttc caccacggag gccagcatga acaacaataa
       61 cacattgtac gtcgggttag atgttcacaa agaatcgatt actgtcgctt atgctatcaa
      121 ttcagaacct gttgaactga tgggtaaaat tggcacatca cctactgata ttcagaatct
      181 ttgtaaacgt ctcaggtcaa agtcatcgca ggtcagtatc gtttatgaag cggggccctg
      241 tggttatgga ctttatcgtc ggctggtgaa atccggtttt gactgcatgg tctgcgcccc
      301 ctcacttatc ccgaaaaaac cgggggagcg ggttaaaacc gatcgccgtg atgccatcag
      361 acttgtgcgt tcactgcgtg caggagatct ttctgctgtc tacgtacccg gcattgaaga
      421 tgaggcattc cgggatttgg cccgggcatg ggcatctgcc cgcgatgatt tacggcatgc
      481 aaggcaacgt ctgaaat
//
LOCUS       DAZSBF010000608          495 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.618, whole genome shotgun sequence.
ACCESSION   DAZSBF010000608 DAZSBF010000000
VERSION     DAZSBF010000608.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.618"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..113)
                     /locus_tag="ACHXWD_004729"
     CDS             complement(<1..113)
                     /locus_tag="ACHXWD_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623344.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFA"
     gene            complement(369..>495)
                     /gene="tdcR"
                     /locus_tag="ACHXWD_004730"
     CDS             complement(369..>495)
                     /gene="tdcR"
                     /locus_tag="ACHXWD_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR7623345.1"
                     /translation="KHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          149 a           94 c           96 g          156 t
ORIGIN      
        1 gcaaactttc tttttaccgt ccaataatca atatttctat tgtgattgtt aaccactgcg
       61 tgcattggcg ggattgaagg gtgaaaaata tgtgcaattg ggaagccttt cattgatgcc
      121 tgccttgtcg tcatgacaaa caataataga tgcttatttt cctcgcaggg ttctttcgca
      181 ctatcaaaaa acgctcattt acccggcgtt acagtaaacg aagcaacatt ctgtggccta
      241 cgacgatcaa aagctctgtg aaaatttccc ttgttgtctt tgtctgacct tatcgcggcg
      301 atcagcaatt attcgttttg tgatcctcaa cacaaaatgc tattcgtcgc ctatgagggg
      361 agaagaattt atggaaaacg attgcattca caaaatgacg tcctgtcaga agagaaaaag
      421 ttgacgttaa tcaattgttc gagatgatca atacctgtat tcgaaatttt aaaatattga
      481 taaaataaat gtttg
//
LOCUS       DAZSBF010000609          492 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.619, whole genome shotgun sequence.
ACCESSION   DAZSBF010000609 DAZSBF010000000
VERSION     DAZSBF010000609.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.619"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(289..351)
                     /locus_tag="ACHXWD_004731"
                     /pseudo
     CDS             complement(289..351)
                     /locus_tag="ACHXWD_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          155 a          118 c          106 g          113 t
ORIGIN      
        1 aaacagaaag gctcacgtgg cataaggcgc ggacaggggt atcgcttagt ttttcaattt
       61 cctatacgtg aacgcccgcc tgcgggcact gttgcagacg caacaggaca ctatccacca
      121 gatggctgaa tacaaccgcc tgctctcaca gcggatggcg gcttatgctt ccgaaatcaa
      181 ccgactgaag gcgctggttg cgaaactgca gcgtatgtag ttcggtaaaa gctcagaaaa
      241 acttcgcgca aaaaccgaac ggcagattcc attttcacgg gcgatctacg ccacacaggc
      301 tctgggttgc agtgactgct ccattaaggc gattttgaac tcataaggga agtaacaaaa
      361 aaactgcaaa agtacaataa ttatatatgg ccaaactaac ctctattaaa aaaacatcag
      421 tttttcacac ttagttgagc ttcatgaaaa aatcatcgag gatttcattg cagaatacac
      481 attccactaa ct
//
LOCUS       DAZSBF010000610          491 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.620, whole genome shotgun sequence.
ACCESSION   DAZSBF010000610 DAZSBF010000000
VERSION     DAZSBF010000610.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.620"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          169 a           97 c           90 g          135 t
ORIGIN      
        1 tcatagttga ttcgattaga agctaatctc atagaaagct ctgtagcgag ttttaacccc
       61 atttttaggg gtaacctata gggatgtact aaccacctta aaataaaggg gtaaaatcgc
      121 ttagaatgcg ttacagagcg tctgagaagc catattcaca gattttacta aaacctcaga
      181 cttctaagta gtcttttaac aggaggggtt cccctatggt cacatatccc ctctctttcc
      241 actaagtaag ccttagcagc acctaagcaa caattgacta acagattcgg tagagtctat
      301 ctcataggtc aacttcataa actcagagct aagttacctt gaaagattct accaagaact
      361 acacaggtgt tatttagtct gactgtgaag ggatatttaa cgggagaaga gatacgatat
      421 aacccgaagg gagagtatca tcttaacaat tatcttaaga ataaaaactg aaaaactctt
      481 aacagataac c
//
LOCUS       DAZSBF010000611          487 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.621, whole genome shotgun sequence.
ACCESSION   DAZSBF010000611 DAZSBF010000000
VERSION     DAZSBF010000611.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.621"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>487)
                     /locus_tag="ACHXWD_004732"
     CDS             complement(<1..>487)
                     /locus_tag="ACHXWD_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HFR7623346.1"
                     /translation="PASLTQLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISL
                     KELHVSNNQLCSLPVLPELLETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPH
                     NIHSIWAIDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSH
                     AL"
BASE COUNT          150 a           73 c          118 g          146 t
ORIGIN      
        1 agagcatggg atgacaatgg gttatcacta atatctattg aacattcatt cctcagatta
       61 agaatgcttt ccgggatatg acttatctga tttatgtcaa aataaccgtt ccttaaattt
      121 tccggcaggt atggaatatc ggttaacata ttgtcgattg cccatatgga gtgaatgttg
      181 tgaggtagag gggggagttc actaagaaga ttccctattg cgctaatctc ttgtaaagaa
      241 aatggtaaag gtggtaaaac tgccagccca ttacatgata catctaatgt ttccagtagt
      301 tctggtaaaa caggaagaga acataattga ttatttgaga catgaagctc cttcaaagat
      361 atggggagtg tgggtagtgt gattagttga ttgtgggacg cattcaataa tttaagtcct
      421 tgaggcaaag caggcagttc aataagtctg ttatagctga cattaagctg tgtaagggac
      481 gcaggca
//
LOCUS       DAZSBF010000612          484 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.622, whole genome shotgun sequence.
ACCESSION   DAZSBF010000612 DAZSBF010000000
VERSION     DAZSBF010000612.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.622"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>484
                     /locus_tag="ACHXWD_004733"
     CDS             <1..>484
                     /locus_tag="ACHXWD_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFR7623347.1"
                     /translation="VDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAAC
                     GDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNV
                     TNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLILK
                     G"
BASE COUNT          159 a           89 c           98 g          138 t
ORIGIN      
        1 gttgattaca aaccccatca agatgattat tcttcagctg aaaaatttct cactcacttc
       61 aacaaaaaat gcaaaaacca gactttggcg ttgatttcca gccgtcctga ggggcgttgc
      121 gttgctgcct gcggtgattt cgggctagtt atgaaagcat attttgacaa gatggaatca
      181 aatggcatca gtgttatggc agccatatta ctggtggata accatgcttt gacggtccgg
      241 ctaagaataa agaacacaac tgaaggatgt acccattacg tggtttcggt ttatgatcct
      301 aatgtaacta acgacaaaat aagaatcatg agcgaaagca aagaggatat taaacactat
      361 tctctgatgg attttatgaa tgtagattat agcctcctga aatggtcaaa tgatcatgtt
      421 attaaccaat ctgttgcaat aattccagca cttccgaaag aacagctatt gatattaaaa
      481 ggat
//
LOCUS       DAZSBF010000613          476 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.623, whole genome shotgun sequence.
ACCESSION   DAZSBF010000613 DAZSBF010000000
VERSION     DAZSBF010000613.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.623"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHXWD_004734"
     rRNA            <1..>476
                     /locus_tag="ACHXWD_004734"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZSBF010000614          474 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.624, whole genome shotgun sequence.
ACCESSION   DAZSBF010000614 DAZSBF010000000
VERSION     DAZSBF010000614.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.624"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>474
                     /locus_tag="ACHXWD_004735"
     CDS             <1..>474
                     /locus_tag="ACHXWD_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR7623348.1"
                     /translation="IHFYDYSRRYAISENIEKDAVTNNIALMNTFYDSDNTSGEVLNN
                     DFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEMRSS
                     HYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLVNLKTLLN"
BASE COUNT          151 a           73 c           96 g          154 t
ORIGIN      
        1 ttattcattt ttatgattat agcaggcgat atgcaattag tgagaatatc gaaaaagacg
       61 ctgtaacaaa caatatcgca ttgatgaaca ctttttatga cagtgataat acgtcaggtg
      121 aggttttaaa taatgatttt acagaagaag agccatttga aactgcgtta gatgtagagc
      181 atacaatagt gcttggattt gctgatgata attttgaaac aaaacctgtt cattatcagg
      241 atttattaac acgatttagt gcctttcaag ataccgtgtt taatctgttt cctgaaatgc
      301 gctcttcgca ttaccacgat atttgtagcg tatgtgtgga tatgacaaag ggaacgcaat
      361 gcatgattca cttgatggtg aatgaagaag ttttcatgtc tctgcctgtt ccagttgcca
      421 cgatggtacg tgaagatgca agtaatttag tcaatcttaa aacactactc aatg
//
LOCUS       DAZSBF010000615          473 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.625, whole genome shotgun sequence.
ACCESSION   DAZSBF010000615 DAZSBF010000000
VERSION     DAZSBF010000615.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.625"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>473)
                     /locus_tag="ACHXWD_004736"
     CDS             complement(<1..>473)
                     /locus_tag="ACHXWD_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR7623349.1"
                     /translation="VYTFILSVLIYREVKWRHLPKLILESVVTTSIVLLLIGFSVGMS
                     WAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMDMTPAVLIFTPIFLPIA
                     QELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKIQHLIKPLLPFYAS"
BASE COUNT          162 a          107 c           98 g          106 t
ORIGIN      
        1 tgctagcata aaatggcagc aatggtttga ttaaatgttg aatcttcaca cccgagatag
       61 aacaaccaac aaatagcgca ctacccaccg gcggtgttaa cagcccaata caaaggttgg
      121 ccaccatcat aataccgaaa tgtactgggt ccattcccag ctcttgtgca atcggcaaga
      181 agatcggtgt aaagattagt acagccggtg tcatgtccat aaagatgccg acaattaata
      241 agacgatatt aatcagcagc aatatgatca gtgggttatc agaaatcccc atcaatgcgt
      301 cgctgatcat ataaggaata tcagcattag tcattgccca ggacatgccc acggaaaaac
      361 cgatcaacag taatacaatc gaagtggtaa caaccgactc cagaatcagt ttgggtaaat
      421 ggcgccattt tacttcccgg taaatcagca ccgagagaat aaatgtgtag aca
//
LOCUS       DAZSBF010000616          472 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.626, whole genome shotgun sequence.
ACCESSION   DAZSBF010000616 DAZSBF010000000
VERSION     DAZSBF010000616.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.626"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHXWD_004737"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHXWD_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZSBF010000617          462 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.627, whole genome shotgun sequence.
ACCESSION   DAZSBF010000617 DAZSBF010000000
VERSION     DAZSBF010000617.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.627"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            163..363
                     /locus_tag="ACHXWD_004738"
                     /pseudo
     CDS             163..363
                     /locus_tag="ACHXWD_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          140 a          107 c          111 g          104 t
ORIGIN      
        1 actcacgtgg gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc
       61 tccggcagaa gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg
      121 agttcacaga taaaacactc tccaggaaac ccggggcggt tcaaccttca cgataaaaaa
      181 cttttcatta aaggtaaagc ggatcaatat ccggcacttc attctctgat atctccgacc
      241 cgcattaatc tgaaagaaat agaaattcac tggcgtgaag tgctacgtct ggcgacctca
      301 ataaaacagg gaacagtgac agcatccctg atgctgagaa aacttgccag ttaccccaaa
      361 caaaagtaag gcagtatgac tttccgtgag acagatgctg gccaacgtgg gatatttaac
      421 gcgggttaac atcgttgtca gagccgcatg ggtgagctgt tg
//
LOCUS       DAZSBF010000618          462 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.628, whole genome shotgun sequence.
ACCESSION   DAZSBF010000618 DAZSBF010000000
VERSION     DAZSBF010000618.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.628"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>462
                     /locus_tag="ACHXWD_004739"
     CDS             <1..>462
                     /locus_tag="ACHXWD_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR7623350.1"
                     /translation="ILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDNTLILSDN
                     GGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPEELRLSPHRYL
                     ATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGRTQTFHREA"
BASE COUNT           85 a          143 c          154 g           80 t
ORIGIN      
        1 catcctctcc cgcacctaca gcagttaccg gacaaaaacg cccgcgccgg tggggagcct
       61 cggccccggc tggaaaatgc ctgcggatat ccgcttacag ctgcgcgata acacactgat
      121 actcagtgat aacggcggca gaagcctgta ttttgagcac ctgtttcccg gtgaggacgg
      181 ttacagccgc agcgaatcac tctggctggt gcgcggcggc gtggcgaaac tggatgaagg
      241 tcaccggctg gccgcactct ggcaggcgct gccggaagaa ctccgcttaa gtccgcatcg
      301 ttatctggcg acaaacagtc cgcaggggcc gtggtggctg ctcggctggt gtgagcgggt
      361 gccggaagcg gatgaggtgc tgcctgcgcc gctgccgccg taccgggtac tgaccgggct
      421 ggtggaccgc ttcgggcgca cacagacgtt ccaccgcgaa gc
//
LOCUS       DAZSBF010000619          461 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.629, whole genome shotgun sequence.
ACCESSION   DAZSBF010000619 DAZSBF010000000
VERSION     DAZSBF010000619.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.629"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>461
                     /locus_tag="ACHXWD_004740"
     CDS             <1..>461
                     /locus_tag="ACHXWD_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7623351.1"
                     /translation="LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTL
                     RSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQ
                     GASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          134 a           94 c          143 g           90 t
ORIGIN      
        1 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg cggatctgta
       61 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaactgag
      121 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      181 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      241 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      301 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      361 acaacttgtg aaattaccga cgagaaggga aagggccttc ccgagagtgg taaaggagag
      421 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAZSBF010000620          459 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.631, whole genome shotgun sequence.
ACCESSION   DAZSBF010000620 DAZSBF010000000
VERSION     DAZSBF010000620.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.631"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            5..459
                     /locus_tag="ACHXWD_004741"
                     /pseudo
     CDS             5..459
                     /locus_tag="ACHXWD_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312962.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          173 a           94 c           71 g          121 t
ORIGIN      
        1 tcgtattcct cgtagttctt tctctgcaaa tattaataat acagcccaga caaatgaaca
       61 ccaaaccctg agtgaattgt tttataaaga actcgaggat aaattttctg gcaaggagct
      121 ggcgacgcct ctattaaaaa gcttctcaga gaactgtaga caaaatggtc gtcatatctt
      181 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
      241 aattacgata attaataaaa cgaaaacacg acactcactc aaaccattgc tccaatattt
      301 gaagaatacc taatggaaat tttacctcaa cgctcagaca ctcttgataa acaagaatta
      361 aacctaaaat cagatagaaa agaaaaagaa ttcccaagaa ttaaacttaa tggtcaatgc
      421 tattttccgg ggcgacccca aaaccgtata gtatgccga
//
LOCUS       DAZSBF010000621          455 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.632, whole genome shotgun sequence.
ACCESSION   DAZSBF010000621 DAZSBF010000000
VERSION     DAZSBF010000621.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.632"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..159
                     /locus_tag="ACHXWD_004742"
     CDS             <1..159
                     /locus_tag="ACHXWD_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR7623352.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
BASE COUNT          119 a          109 c           80 g          147 t
ORIGIN      
        1 tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg tactggtcat
       61 attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc aatttctatt
      121 tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag aaatcggcta
      181 acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg tttctatgtc
      241 cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata actacatcca
      301 ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta cataacatca
      361 tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc aaactctccc
      421 ctacaacgta ttggacaaga aaaaggtatc gctat
//
LOCUS       DAZSBF010000622          454 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.633, whole genome shotgun sequence.
ACCESSION   DAZSBF010000622 DAZSBF010000000
VERSION     DAZSBF010000622.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.633"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHXWD_004743"
     CDS             <1..386
                     /locus_tag="ACHXWD_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR7623353.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           95 c          122 g          104 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agca
//
LOCUS       DAZSBF010000623          447 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.634, whole genome shotgun sequence.
ACCESSION   DAZSBF010000623 DAZSBF010000000
VERSION     DAZSBF010000623.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.634"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>447
                     /locus_tag="ACHXWD_004744"
     CDS             <1..>447
                     /locus_tag="ACHXWD_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR7623354.1"
                     /translation="RWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFNSET
                     VQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRSPES
                     NGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSA"
BASE COUNT          111 a          107 c          145 g           84 t
ORIGIN      
        1 agcgatggtg ctctgacggg ttcgagttcc gctgtgataa cggagaaaaa ctgcgagtca
       61 cgttcgcgct ggactgctgt gaccgtgagg cactgcactg ggcggtcact acgggcggct
      121 tcaacagtga aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg
      181 agcttccggc gtctccagta gagtggctga cggataatgg ttcatgctac cgggctaatg
      241 aaacacggca gtttgcccgg atgttggggc ttgaaccgaa gagcacggcg gtgcggagtc
      301 cggagagtaa cggcatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagtg
      361 tcatgcccaa accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt
      421 ataacgaatg gcatccgcat agtgcac
//
LOCUS       DAZSBF010000624          444 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.635, whole genome shotgun sequence.
ACCESSION   DAZSBF010000624 DAZSBF010000000
VERSION     DAZSBF010000624.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.635"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>444
                     /locus_tag="ACHXWD_004745"
     rRNA            <1..>444
                     /locus_tag="ACHXWD_004745"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          123 a           90 c          139 g           92 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggca
//
LOCUS       DAZSBF010000625          442 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.636, whole genome shotgun sequence.
ACCESSION   DAZSBF010000625 DAZSBF010000000
VERSION     DAZSBF010000625.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.636"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHXWD_004746"
     CDS             <1..>442
                     /locus_tag="ACHXWD_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR7623355.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZSBF010000626          431 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.637, whole genome shotgun sequence.
ACCESSION   DAZSBF010000626 DAZSBF010000000
VERSION     DAZSBF010000626.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.637"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..87)
                     /locus_tag="ACHXWD_004747"
     CDS             complement(<1..87)
                     /locus_tag="ACHXWD_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623356.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVD"
     gene            105..296
                     /locus_tag="ACHXWD_004748"
     CDS             105..296
                     /locus_tag="ACHXWD_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR7623357.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          104 a          110 c           82 g          135 t
ORIGIN      
        1 atccactgac gtttttttac ataacgaaga attgaccatt ttgtcctgtt gtgccttaat
       61 gtaagtaccg tccacagcgt gggacatact tcaaggaacc ttttgtgagt caggcaacca
      121 gtatgcgaaa acgacaccga tttaacagtc gcatgacccg tatcgtactg ctcatcagct
      181 ttatcttctt ctttggccgt tttatctact cgtccgtcgg tgcctggcag caccatcaga
      241 gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc accggtacaa cgttagcggt
      301 taccttcttc actttcacag aacataacgg cacttcgctg tcggatgctt ttgctctttg
      361 ggattatcaa agcggcagat attctttcat cttaaatttt acgtctttgt cctgactgat
      421 gtttatcctg t
//
LOCUS       DAZSBF010000627          420 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.638, whole genome shotgun sequence.
ACCESSION   DAZSBF010000627 DAZSBF010000000
VERSION     DAZSBF010000627.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.638"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHXWD_004749"
     tRNA            41..116
                     /locus_tag="ACHXWD_004749"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZSBF010000628          408 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.639, whole genome shotgun sequence.
ACCESSION   DAZSBF010000628 DAZSBF010000000
VERSION     DAZSBF010000628.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.639"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHXWD_004750"
     tRNA            54..130
                     /locus_tag="ACHXWD_004750"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHXWD_004751"
     tRNA            173..248
                     /locus_tag="ACHXWD_004751"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZSBF010000629          407 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.640, whole genome shotgun sequence.
ACCESSION   DAZSBF010000629 DAZSBF010000000
VERSION     DAZSBF010000629.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.640"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>407)
                     /locus_tag="ACHXWD_004752"
     CDS             complement(<1..>407)
                     /locus_tag="ACHXWD_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR7623358.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINH"
BASE COUNT          133 a           66 c           56 g          152 t
ORIGIN      
        1 atatgattta tcacggagca tgcatacttt atcttttcag aatcaccaaa atattgcatt
       61 cttttattaa aacaccaatc cagtattctt acatcattaa tatatgggga ttttaaaaaa
      121 gtctccagaa tcgaatcgat gaccggtgta aggttaactt ttgcatcatc taaggaatca
      181 ataagaatat gagacaggtt aactttgatg tcattaattt cattgtcagt agctttctta
      241 ttattaagaa gataattatt tactgcgtta aggtcatcta tttctttcag tatccctttt
      301 tcaccaagca agtatgaaat cgattcatta catacataag gcattaatag cagatgttct
      361 ttccttattg ctaataatgc attgttcaat ggtttttcta ataaatc
//
LOCUS       DAZSBF010000630          406 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.641, whole genome shotgun sequence.
ACCESSION   DAZSBF010000630 DAZSBF010000000
VERSION     DAZSBF010000630.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.641"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(4..>406)
                     /locus_tag="ACHXWD_004753"
     CDS             complement(4..>406)
                     /locus_tag="ACHXWD_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR7623359.1"
                     /translation="EQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAAHLAA
                     YAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMSQGKR
                     HNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT           85 a          110 c          136 g           75 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttct
//
LOCUS       DAZSBF010000631          403 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.642, whole genome shotgun sequence.
ACCESSION   DAZSBF010000631 DAZSBF010000000
VERSION     DAZSBF010000631.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.642"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>403
                     /locus_tag="ACHXWD_004754"
     CDS             <1..>403
                     /locus_tag="ACHXWD_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623360.1"
                     /translation="ILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALS
                     ESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLAS
                     FACVSFGFPVILVGGAILLTGIVCTVVLNEID"
BASE COUNT          107 a           69 c           95 g          132 t
ORIGIN      
        1 aatacttgga gagaaatata taaaaatcac aacatccagc ccctggcttt tagaaaagct
       61 cggtaaatat attttctctt cacgagcgcc tcaagttctg gaactcgcta ttggctggcg
      121 aggagcttta tctgaatcaa taaaaggagt taagttctgt atctggtttt cagtagcctg
      181 gagaaccata gaatttatta tgagttcaga gcgagatctg gttaattttc ttggcgattt
      241 ttcaatggat gtggccaaag cagttatagc cggtggtgtt gcaaccgcta ttggaagcct
      301 ggcttctttt gcctgtgtta gctttggctt tccagtaatt cttgtcggag gagcaatttt
      361 actgacaggg atagtgtgta cagttgtttt aaatgaaatc gat
//
LOCUS       DAZSBF010000632          402 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.643, whole genome shotgun sequence.
ACCESSION   DAZSBF010000632 DAZSBF010000000
VERSION     DAZSBF010000632.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.643"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            126..338
                     /locus_tag="ACHXWD_004755"
     CDS             126..338
                     /locus_tag="ACHXWD_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR7623361.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          119 a           87 c          123 g           73 t
ORIGIN      
        1 aagcctccat cagagaggag gcagggaaat aaggcttaaa aggaaataac ttccgcttcc
       61 gaaaggaagc cagtccactg aattatttat cacaataata ctgacaacta aagggggaca
      121 agaaaatgaa tggattcaga aacagttcac ggaacggcca ggtctggcgt taccagcgtg
      181 ccggcgggcg ggccgttatt ctggaagtca gtggacgctg gatggaagcg gcagaagcat
      241 ggcgacgggc tgcctgtgtt gcgcccagga cagactggca gcagtttgcc cgaaaaaggg
      301 ctgaacactg ccaccggcgc tgccggggaa gggtgtaacg aaaaaagcag ccttcattac
      361 tccaggagtc agaaggctga aaggagttta tgagatttgc tt
//
LOCUS       DAZSBF010000633          399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.644, whole genome shotgun sequence.
ACCESSION   DAZSBF010000633 DAZSBF010000000
VERSION     DAZSBF010000633.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.644"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..>399)
                     /locus_tag="ACHXWD_004756"
     CDS             complement(<1..>399)
                     /locus_tag="ACHXWD_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR7623362.1"
                     /translation="KVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGF
                     IGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSA
                     DLFLETTDLPCWRHGEWLHLCSIALSAEPSR"
BASE COUNT          105 a          120 c           89 g           85 t
ORIGIN      
        1 acgcgaaggc tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca
       61 gcagggcaag tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa
      121 tgaacgttcc ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc
      181 ttgcttcaga tacgtgatat cgactccctc agttagcagc gttctttgca ttaacgcccc
      241 aaaaggatca tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc
      301 caccgcaacg ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg
      361 caagagatct acgaccgcat cccctaaaac ccatacttt
//
LOCUS       DAZSBF010000634          399 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.645, whole genome shotgun sequence.
ACCESSION   DAZSBF010000634 DAZSBF010000000
VERSION     DAZSBF010000634.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.645"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>399
                     /locus_tag="ACHXWD_004757"
     CDS             <1..>399
                     /locus_tag="ACHXWD_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR7623363.1"
                     /translation="GQSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRS
                     LNDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAG
                     RINSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          104 a           64 c          112 g          119 t
ORIGIN      
        1 ggtcaatcta aaagtcttgg tgaatctctt ggtggcttga tgagcgatat gtcgcatggg
       61 gtcgattcac taaccgatat tagttacagg gtacgagcgt tctttgatga ggtgacatta
      121 gcttatcgtt cactaaatga cactcaaaaa gctgtagctg atggtcttgc aaatggcctg
      181 ttaagtgcat taaaggggct tgctggtata gtcgctgtac ggtctgggat tggtgctgta
      241 ggtggtatct ggaacttaat tagggccatt tccacgcttg gaaatgtggc aaatacagca
      301 gcaggaagaa tcaattcacg ttctactggt agtgttgaag gtggaaaaag taggctatct
      361 gtagctgatg caattaccag agtaatgatt gtcggtatg
//
LOCUS       DAZSBF010000635          395 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.646, whole genome shotgun sequence.
ACCESSION   DAZSBF010000635 DAZSBF010000000
VERSION     DAZSBF010000635.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.646"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          126 a          104 c           67 g           98 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcata
//
LOCUS       DAZSBF010000636          395 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.647, whole genome shotgun sequence.
ACCESSION   DAZSBF010000636 DAZSBF010000000
VERSION     DAZSBF010000636.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.647"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHXWD_004758"
     CDS             complement(<1..356)
                     /locus_tag="ACHXWD_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR7623364.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTA"
BASE COUNT          103 a           99 c          117 g           76 t
ORIGIN      
        1 tgcgctgttt tcgtgaacac gcggcggaca gcctcacttc ccaggcgctg gcgggcctga
       61 ataacggcac tgggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
      121 acaatctggt gaagaggttc tttacgctca agcgccatgc caacaataca ccagaccatc
      181 atttcgaggg gaagacggcg cttgcgtagc gttacagtac ctgattcggc aaggcaacga
      241 gagatgagtt cggggtcgag gtaatccccc agagaagtca gtgggttacg cagagaatcg
      301 taacgggata ccagatcaag agcctgtcca atgtgcataa aaaaatccgg aaacaagtga
      361 gcgtttccgg attcttacac agccactgga tcggt
//
LOCUS       DAZSBF010000637          394 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.648, whole genome shotgun sequence.
ACCESSION   DAZSBF010000637 DAZSBF010000000
VERSION     DAZSBF010000637.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.648"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            153..>394
                     /locus_tag="ACHXWD_004759"
     CDS             153..>394
                     /locus_tag="ACHXWD_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR7623365.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT          122 a           77 c           68 g          127 t
ORIGIN      
        1 attgtttttc aatgaaaaaa tattattctc gtaatatctc acgataaata acattaggat
       61 tttgttattt aaacacgagt cctttgcact tgcttacttt atcgataaat cctacttttt
      121 taatgctatc caatcatttt aaggagttta aaatggataa gaagcaagta acggatttaa
      181 ggtcggaact actcgattca cgttttggtg cgaagtctat ttccactatc gcagaatcaa
      241 aacgttttcc gctgcacgaa atgcgcgacg atgtcgcatt ccagattatc aatgacgaat
      301 tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc tgggacgacg
      361 aaaatgtcca caaattgatg gatttatcca ttaa
//
LOCUS       DAZSBF010000638          394 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.649, whole genome shotgun sequence.
ACCESSION   DAZSBF010000638 DAZSBF010000000
VERSION     DAZSBF010000638.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.649"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>394
                     /locus_tag="ACHXWD_004760"
     CDS             <1..>394
                     /locus_tag="ACHXWD_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR7623366.1"
                     /translation="VAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDKR
                     DDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKISE
                     FGSLDYGRNYGVGYDIGSWTDMLPEFGGD"
BASE COUNT          105 a           74 c          103 g          112 t
ORIGIN      
        1 gttgcggtgg ttgtaactgg tttgttagct gcgaacgtag cacacgctgc cgaagtctat
       61 aacaaggatg gtaataaact cgacctttat ggcaaggtta ccgctctacg ttattttact
      121 gatgataagc gtgacgatgg tgataaaact tatgcccgtc tcggctttaa aggagaaacg
      181 caaatcaatg atcaaatgat tggttttggt cactgggaat atgattttaa aggctataac
      241 gatgaagcca acggctcgcg cggcaacaag acccgtctgg cctatgctgg tttaaaaatt
      301 agtgaatttg gctctctgga ctatggtcgt aactacggtg tcggctacga cattggttca
      361 tggaccgata tgttgccaga atttggtggc gata
//
LOCUS       DAZSBF010000639          394 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.650, whole genome shotgun sequence.
ACCESSION   DAZSBF010000639 DAZSBF010000000
VERSION     DAZSBF010000639.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.650"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(85..291)
                     /locus_tag="ACHXWD_004761"
     CDS             complement(85..291)
                     /locus_tag="ACHXWD_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR7623367.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          106 a           98 c           81 g          109 t
ORIGIN      
        1 taaatattcc tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc gctggcggtt
       61 ttcaatgtat tcagaactca gcaatcagta aaaatcaccg gttgcctttt cggcctgatc
      121 catccacacc ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg
      181 ggcgcggtct ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata
      241 tgtacggcgt aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc
      301 ccccttttgt atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa
      361 atagtaccgc cttttgtgta attttactac tcat
//
LOCUS       DAZSBF010000640          393 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.651, whole genome shotgun sequence.
ACCESSION   DAZSBF010000640 DAZSBF010000000
VERSION     DAZSBF010000640.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.651"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            62..>393
                     /gene="yhaV"
                     /locus_tag="ACHXWD_004762"
     CDS             62..>393
                     /gene="yhaV"
                     /locus_tag="ACHXWD_004762"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR7623368.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          120 a           72 c           99 g          102 t
ORIGIN      
        1 gaaagaaact tgtcactggc atggacgtca acattgatga tgagattggc gacgacgaat
       61 aatggatttt ccacaaaggg ttaatggttg ggcgctatat gctcatccct gttttcagga
      121 aacctacgac gctttagttg ccgaagtcga gacattaaag ggaaaagatc ctgaaaatta
      181 tcagagaaaa gccgccacaa agttattggc ggtagtccat aaagtgattg aggagcatat
      241 cacggtcaat ccatcatcac cggcattccg tcatggcaag tcgttaggct ctgggaaaaa
      301 taaagactgg tcacgggtaa aatttggtgc aggtcgttat cgtctcttct ttcgttatag
      361 tgaaaaagag aaagtcatca ttctgggatg gat
//
LOCUS       DAZSBF010000641          392 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.652, whole genome shotgun sequence.
ACCESSION   DAZSBF010000641 DAZSBF010000000
VERSION     DAZSBF010000641.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.652"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
BASE COUNT          118 a           68 c           81 g          125 t
ORIGIN      
        1 tcacataaac caccgattga gtgacagtta ctgaaacaac tatttcgaca caaaatctcg
       61 tctattcaac atcatgcaaa tttgtcagtg actgaattta gtgacagaac cctctggaat
      121 tctgccgtgt aaccacttat atgcagacct gccccgttgc tctgggtttt caggctgcga
      181 tgagtgatga gcgatgattt ccatacagat gatatatcga ctggaattga ttgcctgcaa
      241 ttagatttag ttttattgtg tggatcgttt tagttagtgg ggatgaattt ccgataaatt
      301 aaccaatgga aaggagatga tatgaataaa aagataactg gattattaat aattagtctg
      361 gcgtcaattt tcttaattga ttgcacccag tg
//
LOCUS       DAZSBF010000642          389 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.653, whole genome shotgun sequence.
ACCESSION   DAZSBF010000642 DAZSBF010000000
VERSION     DAZSBF010000642.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.653"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            18..125
                     /locus_tag="ACHXWD_004763"
     CDS             18..125
                     /locus_tag="ACHXWD_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR7623369.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT           94 a           85 c          126 g           84 t
ORIGIN      
        1 acaatacagg cggctatatg acgctcgcag aactgggcat ggccttctgg catgatttag
       61 cggctccggt cattgctggt attcttgcca gtatgatcgt gaactggctg aacaagcgga
      121 agtaacgtgc catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg gatcaggccg
      181 caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca agcggataat
      241 attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca gaaaaacagt
      301 cgtagaatca gcgtcaagcc ggaagggttc cggtgaggcg caatgttgcg ggggctttat
      361 ccctggtggc attggttgct ggagagaga
//
LOCUS       DAZSBF010000643          388 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.654, whole genome shotgun sequence.
ACCESSION   DAZSBF010000643 DAZSBF010000000
VERSION     DAZSBF010000643.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.654"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            3..>388
                     /gene="ospZ"
                     /locus_tag="ACHXWD_004764"
     CDS             3..>388
                     /gene="ospZ"
                     /locus_tag="ACHXWD_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HFR7623370.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYGK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELP"
BASE COUNT          137 a           65 c           73 g          113 t
ORIGIN      
        1 aaatgattag tcccatcaag aatattaaga acgtatttcc aataaatact gcaaatactg
       61 aatatatagt gagaaatatt tatcctagag ttgaacatgg ttactttaat gaatcaccaa
      121 acatatatgg caagaagtat atctctggta taaccagaag tatggcacaa ttaaaaatag
      181 aggagtttat taatgagaaa tccagacgac ttaattatat gaagacaatg tattctccat
      241 gtccggaaga ttttcagcct atttccagag acgaagccag tacaccggaa ggaagctggt
      301 tgacagttat atctggaaaa cgtcctatgg ggcaattttc tgtggatagt ttgtaccatc
      361 ctgacttaca tgcattatgt gagctccc
//
LOCUS       DAZSBF010000644          386 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.655, whole genome shotgun sequence.
ACCESSION   DAZSBF010000644 DAZSBF010000000
VERSION     DAZSBF010000644.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.655"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>386
                     /locus_tag="ACHXWD_004765"
     CDS             <1..>386
                     /locus_tag="ACHXWD_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623371.1"
                     /translation="LYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNR
                     FVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIV
                     IPLMIIPMLGSNTVQDLMNTIINMTA"
BASE COUNT           81 a           98 c          102 g          105 t
ORIGIN      
        1 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
       61 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
      121 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
      181 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
      241 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
      301 gatcgtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
      361 gaatactatt attaatatga ccgccg
//
LOCUS       DAZSBF010000645          378 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.657, whole genome shotgun sequence.
ACCESSION   DAZSBF010000645 DAZSBF010000000
VERSION     DAZSBF010000645.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.657"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>378
                     /locus_tag="ACHXWD_004766"
     CDS             <1..>378
                     /locus_tag="ACHXWD_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR7623372.1"
                     /translation="NNTHSSVSCSPSINSNSTSNEYYLRILTEWEKNSSPGEERGIAF
                     NRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKELNADN
                     NRLSVIPELPESLTTLSVRSNQL"
BASE COUNT          123 a           83 c           57 g          115 t
ORIGIN      
        1 taaataatac acactcatca gtttcttgct ccccctctat taactcaaac tcaaccagta
       61 atgaatatta tctgagaatc ctgactgaat gggaaaagaa ctcttctccc ggggaagagc
      121 gaggcattgc ttttaacaga ctctcccagt gctttcagaa tcaagaagca gtattaaatt
      181 tatcagacct aaatttgacg tctcttcccg aattaccaaa gcatatttct gctttgattg
      241 tagaaaataa taaattaaca tcattgccaa agctgcctgc atttctcaaa gaacttaatg
      301 ctgataataa caggctttct gtgataccag aacttcctga gtcattaaca actttaagtg
      361 ttcgttctaa tcaactgg
//
LOCUS       DAZSBF010000646          373 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.658, whole genome shotgun sequence.
ACCESSION   DAZSBF010000646 DAZSBF010000000
VERSION     DAZSBF010000646.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.658"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..97
                     /locus_tag="ACHXWD_004767"
     CDS             <1..97
                     /locus_tag="ACHXWD_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623373.1"
                     /translation="NNLEPDTIAEQFSKKMKKTFIEIINRFNHFL"
BASE COUNT          120 a           60 c           75 g          118 t
ORIGIN      
        1 gaacaatctg gaaccggata ctatagcaga acaattttca aagaagatga aaaaaacgtt
       61 tattgaaata ataaatagat ttaatcattt tttatgagac tgtaatttaa attgtgtaat
      121 tacctgtttt tgatatgttc actccaacaa ggtgtagtga ccgggtaact gccaactttc
      181 aggcaaccac ctcgattagc aaccttattt ccatatcgag ttcaaatatc ccgtaggggt
      241 ttacatgggc gtaaactgat gattgttcag gtttccatgg tgtggccaaa tcttcttcat
      301 ggataaaggt cacatccaga gggtgagtat gttccaatat ttaatactaa agatatgagg
      361 cttggaattg ggt
//
LOCUS       DAZSBF010000647          368 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.660, whole genome shotgun sequence.
ACCESSION   DAZSBF010000647 DAZSBF010000000
VERSION     DAZSBF010000647.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.660"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>368
                     /gene="virF"
                     /locus_tag="ACHXWD_004768"
     CDS             <1..>368
                     /gene="virF"
                     /locus_tag="ACHXWD_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFR7623374.1"
                     /translation="SYSEEKRGLNKKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLAC
                     LLSAVSDEEALYTSISIASSLSFSDQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKR
                     LESEKLTFQQILLDIRMHHA"
BASE COUNT          121 a           57 c           70 g          120 t
ORIGIN      
        1 ctcctattct gaggagaaaa gggggttaaa caaaaaaata ttcctcctct ctgaggagga
       61 ggtttctatc gatttgttca aatctataaa agagatgcct ttcggcaaaa gaaagatcta
      121 tagtttagct tgccttttat cagctgtttc tgatgaggaa gctttatata cttcgatatc
      181 gatagcttct tctcttagtt tttctgatca gataaggaag attgttgaaa aaaacatcga
      241 gaagagatgg cgtctttctg atatttcaaa taacttgaat ttatcagaaa tagctgttag
      301 aaaacgattg gagagtgaaa aattaacatt tcaacaaatc cttcttgata ttcgcatgca
      361 tcatgcag
//
LOCUS       DAZSBF010000648          362 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.661, whole genome shotgun sequence.
ACCESSION   DAZSBF010000648 DAZSBF010000000
VERSION     DAZSBF010000648.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.661"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..>362
                     /gene="ospB"
                     /locus_tag="ACHXWD_004769"
     CDS             <1..>362
                     /gene="ospB"
                     /locus_tag="ACHXWD_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFR7623375.1"
                     /translation="LGLGSELIDVQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNF
                     NNAPAESLSCILNSLPFFKEKESLLEQIKKHLENDESLSDGLKISGYHGYGVHYGQEL
                     FPYSHYRSTSIPADPEHT"
BASE COUNT          110 a           71 c           66 g          115 t
ORIGIN      
        1 attaggcctt ggttcggaac ttattgatgt acaaacaatc atttcaagaa tgaaagactg
       61 tggtattcta aatgtgaaag atatccgttt tacttcatgc ggctccgctg ataaagtggc
      121 tcctaaaaat tttaacaatg cccctgctga aagtctttct tgtatcctta actctctgcc
      181 tttttttaag gaaaaagaat ctttgctaga gcagataaaa aaacaccttg aaaacgatga
      241 gtcattgagt gatggtctaa aaatatccgg ctatcatgga tatggagttc actatggtca
      301 agagcttttt ccctactcac attatcgttc aacttcaatt cctgctgatc cggagcatac
      361 ag
//
LOCUS       DAZSBF010000649          362 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.662, whole genome shotgun sequence.
ACCESSION   DAZSBF010000649 DAZSBF010000000
VERSION     DAZSBF010000649.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.662"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(62..>362)
                     /locus_tag="ACHXWD_004770"
     CDS             complement(62..>362)
                     /locus_tag="ACHXWD_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623376.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          120 a           62 c           65 g          115 t
ORIGIN      
        1 gacgttataa aagacaaata gattgtgatc gcctacagac gatcacaatg aaaggaaaat
       61 attaacggtt tttgctatca ctcaaatgtt tttcatcctg caccatatca ttgccagatt
      121 catgcaaagt atttattaat gcattggcat ctggatattg ttgctgatac ttggttagat
      181 atatatcact atggatatgt tcggcaatta tattgataag caagcgatta ttcgagtata
      241 accccagtga ttcagctcgt tgtgaaaaaa ccattactgt cttcatatca acaacaagca
      301 taaaacggtg agaagggtaa tattcttctt catggttaaa gcggtaatac agttctacac
      361 gt
//
LOCUS       DAZSBF010000650          345 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.denovo.663, whole genome shotgun sequence.
ACCESSION   DAZSBF010000650 DAZSBF010000000
VERSION     DAZSBF010000650.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.denovo.663"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHXWD_004771"
     CDS             <1..81
                     /locus_tag="ACHXWD_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR7623377.1"
                     /translation="IKTRLGFSARELSNITFLVKKINEKI"
     gene            68..>345
                     /locus_tag="ACHXWD_004772"
     CDS             68..>345
                     /locus_tag="ACHXWD_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="may be involved in the export or assemby of
                     proteins involved in the entry of Salmonella into host
                     cells; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFR7623378.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFDRFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGL"
BASE COUNT          123 a           44 c           56 g          122 t
ORIGIN      
        1 ataaaaacac gactaggctt ttctgcaaga gaattatcga atattacttt tttagtgaaa
       61 aaaataaatg aaaaaattta atattaaatc tttgactctc ttgattgtat tgttaccatt
      121 gatagttaat gctaataata ttgactctca tttattagaa caaaatgaca tagcgaaata
      181 tgttgctcaa agtgatacgg taggatcttt ttttgaccgt ttttctgcgt tattaaatta
      241 tcctattgtt gttagcaaac aagctgcaaa aaagagaata tctggggagt ttgatttatc
      301 taatccagaa gaaatgcttg aaaaactgac attacttgtg ggact
//
LOCUS       DAZSBF010000651        24267 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.045, whole genome shotgun sequence.
ACCESSION   DAZSBF010000651 DAZSBF010000000
VERSION     DAZSBF010000651.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24267
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.045"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            561..800
                     /locus_tag="ACHXWD_004773"
     CDS             561..800
                     /locus_tag="ACHXWD_004773"
                     /inference="COORDINATES: protein motif:HMM:NF020425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA family protein"
                     /protein_id="HFR7623379.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
     gene            complement(988..1965)
                     /gene="intI2"
                     /locus_tag="ACHXWD_004774"
                     /pseudo
     CDS             complement(988..1965)
                     /gene="intI2"
                     /locus_tag="ACHXWD_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            2297..2770
                     /gene="dfrA1"
                     /locus_tag="ACHXWD_004775"
     CDS             2297..2770
                     /gene="dfrA1"
                     /locus_tag="ACHXWD_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFR7623380.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISRIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            2865..3389
                     /gene="sat2"
                     /locus_tag="ACHXWD_004776"
     CDS             2865..3389
                     /gene="sat2"
                     /locus_tag="ACHXWD_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HFR7623381.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            3458..3955
                     /locus_tag="ACHXWD_004777"
     CDS             3458..3955
                     /locus_tag="ACHXWD_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623382.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            4016..4387
                     /locus_tag="ACHXWD_004778"
     CDS             4016..4387
                     /locus_tag="ACHXWD_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623383.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            4797..5174
                     /locus_tag="ACHXWD_004779"
     CDS             4797..5174
                     /locus_tag="ACHXWD_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623384.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            5197..5349
                     /locus_tag="ACHXWD_004780"
                     /pseudo
     CDS             5197..5349
                     /locus_tag="ACHXWD_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5405..7021)
                     /locus_tag="ACHXWD_004781"
     CDS             complement(5405..7021)
                     /locus_tag="ACHXWD_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7-like element transposition protein TnsE"
                     /protein_id="HFR7623385.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(7022..8548)
                     /locus_tag="ACHXWD_004782"
     CDS             complement(7022..8548)
                     /locus_tag="ACHXWD_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFR7623386.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(8551..10218)
                     /locus_tag="ACHXWD_004783"
     CDS             complement(8551..10218)
                     /locus_tag="ACHXWD_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR7623387.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(10215..12323)
                     /locus_tag="ACHXWD_004784"
     CDS             complement(10215..12323)
                     /locus_tag="ACHXWD_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR7623388.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(12310..13131)
                     /locus_tag="ACHXWD_004785"
     CDS             complement(12310..13131)
                     /locus_tag="ACHXWD_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFR7623389.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(13291..15120)
                     /gene="glmS"
                     /locus_tag="ACHXWD_004786"
     CDS             complement(13291..15120)
                     /gene="glmS"
                     /locus_tag="ACHXWD_004786"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR7623390.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(15282..16652)
                     /gene="glmU"
                     /locus_tag="ACHXWD_004787"
     CDS             complement(15282..16652)
                     /gene="glmU"
                     /locus_tag="ACHXWD_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR7623391.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(17003..17422)
                     /locus_tag="ACHXWD_004788"
     CDS             complement(17003..17422)
                     /locus_tag="ACHXWD_004788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR7623392.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(17443..18825)
                     /gene="atpD"
                     /locus_tag="ACHXWD_004789"
     CDS             complement(17443..18825)
                     /gene="atpD"
                     /locus_tag="ACHXWD_004789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR7623393.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(18852..19715)
                     /gene="atpG"
                     /locus_tag="ACHXWD_004790"
     CDS             complement(18852..19715)
                     /gene="atpG"
                     /locus_tag="ACHXWD_004790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR7623394.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(19766..21307)
                     /gene="atpA"
                     /locus_tag="ACHXWD_004791"
     CDS             complement(19766..21307)
                     /gene="atpA"
                     /locus_tag="ACHXWD_004791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR7623395.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(21320..21853)
                     /gene="atpH"
                     /locus_tag="ACHXWD_004792"
     CDS             complement(21320..21853)
                     /gene="atpH"
                     /locus_tag="ACHXWD_004792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR7623396.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(21868..22338)
                     /gene="atpF"
                     /locus_tag="ACHXWD_004793"
     CDS             complement(21868..22338)
                     /gene="atpF"
                     /locus_tag="ACHXWD_004793"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR7623397.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(22400..22639)
                     /gene="atpE"
                     /locus_tag="ACHXWD_004794"
     CDS             complement(22400..22639)
                     /gene="atpE"
                     /locus_tag="ACHXWD_004794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR7623398.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(22686..23501)
                     /gene="atpB"
                     /locus_tag="ACHXWD_004795"
     CDS             complement(22686..23501)
                     /gene="atpB"
                     /locus_tag="ACHXWD_004795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR7623399.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(23510..23890)
                     /gene="atpI"
                     /locus_tag="ACHXWD_004796"
     CDS             complement(23510..23890)
                     /gene="atpI"
                     /locus_tag="ACHXWD_004796"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR7623400.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         6319 a         6127 c         5448 g         6373 t
ORIGIN      
        1 ggatcggtca actgatcctt aactgatcgg cattacaccg gtcggcagcc ctgtgggcgg
       61 acaaaatagt tgggaactgg gaggggtgga aatggagttt ttaaggatta tttagggaag
      121 agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc taatacgaaa attaaaaatg
      181 acaaaatagt ttggaactag atttcactta tctggttaac taatctagct aacctgccgc
      241 tcagcttagt acgatcctat atccaactga tcataatcct ttcatcagta tcccaaatta
      301 aatccccctc ctagactgcc tcaccaacac tttacagtga tcccgtattt ccggacaggt
      361 gattgatttt tttcgcttgt acaggtgtta agccacagtt atcttgatgt ggacattatg
      421 cattgtaata gcccattaac atgcgctaag agcataaatt aatatacagt aacaagcatt
      481 acgaggtgct aatgtttgga aagcagcctc ttccaactac aataataagt taactatcct
      541 ccacccacaa ataattacaa atgacccctt tccgctataa ctccgatctc accagcggct
      601 cgctacaaac ccgagagtgt cgcattatta ctggcttgtt actccaagag cttgatgaag
      661 ctgcctggga taaagccatg tataaggaaa atgtgcttca gaaacgcacg caatctacgg
      721 taagacgtat ttcttcagcg cttagaaaac gcctagaaca cctaagctct gacttttggg
      781 cttttgcgtt tttatgttag gcattgtctg gcaactggag gaagtgtaat gaccatgaat
      841 ggtcactgtg attgacagta tcttgcttaa acttatctga acaatggtag aactggctca
      901 tatttatccc ctctatgatt gtttatcttt ttcaccataa aaagtatcag gggctactca
      961 gggctacata aagatattta tctactttta ctgattgata agtagcatca gtccatccgc
     1021 aggactggtg gtgccggcaa aatgctgacc caacacatgc gtatagattt gcgtggtctt
     1081 aacatcgtta tgccctaaga gttcttgcac agtgcgaata tcacgccccg cttgtaatag
     1141 atgcgtagca aacgagtgac gaaatgtatg acaagtgaca cgcttgctaa cgatgcctgc
     1201 tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat gatggcggca
     1261 taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa acatccacgc
     1321 cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg atggccctac
     1381 gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt attttattgc
     1441 tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac cgtcatgcac
     1501 agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc attcattaat
     1561 gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat ccataacctg
     1621 caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag gcttgcttgc
     1681 agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca aaaaagctag
     1741 ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg ctaagctgga
     1801 taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt tgtgaaacag
     1861 aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat aacctttttg
     1921 tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac gctccttgtc
     1981 ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg cctgtttttt
     2041 acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata aattaaaatg
     2101 ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg gcatgcacga
     2161 tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca agtgattata
     2221 ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat aaaatgttaa
     2281 cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga atggagttat
     2341 cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt ttaaagctat
     2401 tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg gagcattacc
     2461 caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg agaacgtatt
     2521 gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg atcatgtcat
     2581 tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata cactacatat
     2641 atctagaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc ccagcaattt
     2701 taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc aaatctggca
     2761 aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt gtaccaaaag
     2821 ccgcgccagg tttgcgatcc gctgtgccag gcgttaggcg tcatatgaag atttcggtga
     2881 tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt cgtgaagtgt
     2941 tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg agcccctacc
     3001 ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat ggcgcattca
     3061 tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atggaacgat ctagcctcta
     3121 tcgaacacat tgttgtgtcg cacacgcacc gaggcaaagg agtcgcgcac agtctcatcg
     3181 aatttgcgaa aaagtgggca ctaagcagac agctccttgg catacgatta gagacacaaa
     3241 cgaacaatgt acctgcctgc aatttgtacg caaaatgtgg ctttactctc ggcggcattg
     3301 acctgttcac gtataaaact agacctcaag tctcgaacga aacagcgatg tactggtact
     3361 ggttctcggg agcacaggat gacgcctaac aattcattca agccgacacc gcttcgcggc
     3421 gcggcttaat tcaggagtta gagagctggg gaagactatg cgcgatctgt tgaaggtggt
     3481 tctaagcctc gtacttgcga tggcatcggg gcaggcactt gctgacctgc caattgtttt
     3541 agtggatgaa gctcgtcttc cctatgacta ctccccatcc aactacgaca tttctccaag
     3601 caactacgac aactccataa gcaattacga caatagtcca tcaaattacg acaactctga
     3661 gagcaactac gataatagtt catccaatta cgacaatagt cgcaacggaa atcgtaggct
     3721 tatatatagc gcaaatgggt ctcgcacttt cgccggctac tacgtcattg ccaacaatgg
     3781 gacaacgaac ttcttttcca catctggcaa aaggatgttc tacaccccaa aaggggggcg
     3841 cggcgtctat ggcggcaaag atgggagctt ctgcggggca ttggtcgtca taaatggcca
     3901 attttcgctt gccctgacag ataacggcct gaagatcatg tatctaagca actagcctgc
     3961 tctctaataa aatgttaggc ctcaacatct agtcgcaagc tgaggggaac cactagtgtc
     4021 atacgaacct ccaagagacg gttacacaaa cgggtacatt gttgatgtca tgtatgacaa
     4081 tcgcccaagt aagtatccag ctgtgttcag aacgtacgtc cgaattccag actatgtcga
     4141 actagataac gcggaggagc tggccgacgc aatcattcga gaggttgagt taaaggaggc
     4201 atggctcaca agggtcttgg ggaactcagc cgatcctgac gcttctgtgg gcaagtggga
     4261 ccggcgccta gcgcagtgcg gtggtattga tgagatgtca agtggggtgc aggcaacata
     4321 cttcagtcca aacggtcagc tggtcggctt agtggcatac attgatgccg aatggatgga
     4381 tgaatagctc tgacgttacg attcaggtga gacaaccaga tgggcattgc gtacaagctc
     4441 agttgccacc gatgtggcta cgagtctgat cttctttacc taggccaagg tatggcgatg
     4501 cttcctgagc aaatagtcgg aacgtgcaca tgctgcgaca atctaacgac gatttctata
     4561 aatgaaagct acggagtatg ctcactttgt ggggctaagg atcgtgtgat atttacaagc
     4621 tatcagcatg aggcaaggcc aagaaatccc tgctatctag agagaagttt caaatatcaa
     4681 tgcccaagat gccatgcctt cagtatggag cctccggatc taccggagat gctttgggac
     4741 taatgctccg ctttgcctcg ggtatccgtt cgacctatgt ttaagaggaa acgcacgtgt
     4801 ctgccgccaa agctcttgcc tatgcacttt gtgacaagtt ggaaagcaaa aaagttgatg
     4861 aagtcgtacg aaatcttgtt tctagcggct ggaatattag ggaagtggcc tgggaagctt
     4921 cgtcctttga tcatgaaatg ccctactttg acacggggcg ggacttcgat attggatttc
     4981 ctgacacgct gtcatccaag attgagtttt tgaccgcgaa tggatcaatc gtccaagcac
     5041 gtgctcgcct gcacttcaag caggctctaa tttttgccaa ggcgtccaaa catgtcgccg
     5101 cgctcaagaa ctcattggat tactactatg gatcgtctgt aatcccccct caaaaccacg
     5161 gaggctattt gtagaaaatt ccattaatat gaactcaagg tgttccctac ggacgaatca
     5221 gcgatgaagg tggtgtacct ggctatccag gcggcgacca agaaatggac gatgccgatc
     5281 cgcaactgga agccggccat gaaccgtttt atgatcgaat ttggtgaccg tttaaatggc
     5341 cacctttaac tgaccggcaa aagcacttac acaaaagggt ttacaggctc tttcagtcat
     5401 tcctttaatg cgtaaattgc tctctaacca ctctctcagc ccacccttcc atatcctctc
     5461 tcgatacttg cagctccccc aacccctttg gatgattcac ccctctatgc ccgtcttgtc
     5521 catataattg atcaaacaaa ctatttggcc aattcaatga gctcttcacc actccacgcc
     5581 gtatcttttc aaaatcattc cgccatgttt cgctatcaac gccactcaac actttggtag
     5641 aaagcatttt caccccatcc gatgcatcca cctctagcaa tacaaattca ctgccattac
     5701 gttgcacccc aacggcttta atcactcttg gtgagccatc tttacacaga tgtaatcggc
     5761 tacgcccaac ttttggcaaa accaaggttt cttcaaaaag cacccgacat gcaaatttag
     5821 tttttagaat tgaaagtagc tcatcaaaag cagcaaatcg attagcaaaa atagagttgt
     5881 aattggttgc atcctgcttc ccacccacat ctgccgctgc taggacacca cctaagtgtg
     5941 gctcatcagt actaaccaaa ttactatttt cacttgttgt tgcttcttct ttttctaaaa
     6001 tagtttggct actttttctt gccctgcgag atttttcaat tcgactaggt ctactaaaac
     6061 ttatccatgt cggctcagcc tctatgactc gtgtgtctgt gtctatattg gctgtttcct
     6121 catcgtccaa ctgtagatgc tcatcatcaa caactgactc tgttgaaacc gctatgtgct
     6181 gcgtactacc atcacctgcc tccttttcct gaaaagaggc atggctaata gctgttgtgc
     6241 taggaagcat agcgttgatt tctaaaccta ctatttcctc aactaaataa tccttatcct
     6301 cgttagaaga acgtcctttt acatgtaatt tccaaccctg catgggcgga gggtcaaagc
     6361 taaagcacca gctttcaacg ccgttcttaa tttctctatt ttgttgatag tgcctaaaaa
     6421 tactttcata cgaatccata acatcttgat ccgaaaacaa ccaaaccaaa agctgcacta
     6481 cggccgactg ctctaacgcc cctttaggaa acgatgaact gggtaagatc cttatctcta
     6541 aatgatctcg ctcaacctca tactgaacgt cgaactcttg ctgtaaagcg gtactgctca
     6601 aacagcttcg acagaaatag gagttaatta aaaataatga ccgagctagc tctaactgtg
     6661 ggatgtgata acgagtcttg ctatatggtg tttcagcttc gtaaataaat gaactctgcg
     6721 ctccatcctt gttgcgtacg gaagcaaagg acgagagatt aggaaaatcg attattttgg
     6781 ctcgttgaag atccgatgct tcaaactcaa tcacacgatc cgccttattt atttgctttg
     6841 ttgtactgtt aaccacctta gctcgactta ataaaggcaa atgagtaaat cgagtccatt
     6901 tccgttcttg cattggatta aaccaaacaa aaatacgcca ctccttatga cccgagttac
     6961 ggaataaatg accaatatgt acaacctgca cattgtcatt aaatgtagct agcctaacca
     7021 ctcactcttc cccataaacc atttccaaga atctttgtgc ttcctcagtt atccgctctt
     7081 tagataacgt tgcacttctt aataatcgcc agcgcctaag ctcaacatct tcctgtttaa
     7141 gcttaataaa agcttggcta atccgtctaa tttgataatc ttccacactc tctgagtaac
     7201 gctttaagca aagcgctacc aaaggcagtt tctgtagatt ttttgcaaga gaggttccgt
     7261 taggagtttg ctttaacagc cagctcgatg tcgctcttgg gtgatcaagg ctactatcta
     7321 gacgctttat gattcttaat agttgtcgta cagcaattcg atctctttgg ttccaatcaa
     7381 ctctaggggc gggtgccaga cgctcttgtt gatgctgttg attccagtga actagccaat
     7441 ccctgtcatg tcggtaaagc caagcgtata gcaccccacc ctctaaagac tgtcttgccg
     7501 ccttaattcc ttggtattta tgcactagtt gctgccagtc tttatgctta acagataaat
     7561 cttcagagtt aggttgcaca gactggctaa caggtcttgt cgttatagag tgctcagtaa
     7621 gagcacttgc ctgctgtagc gcttctataa ccgttagttt tggcaataag gcttgccaca
     7681 caatactatg ctgtaaataa ctaaaggctt ttctatgctt acggaatata cttttcagcc
     7741 aacacgtgtc cttgttctct gccaacttta aatccagttt ctctagtgcc tcatcactaa
     7801 aagtctgcct cactctctcc gccaccaagt catgacgaat gtgcttgctt ttggttagcc
     7861 ctagatcctg cgctaagcgc tgataaaaca gcgtccactg ctcaaggctt ggggaaagct
     7921 cttgcgctcg tggagcatct aacagagggg ctatataagc agctagtgct gttaattgag
     7981 atagggagtc tttggggtag tctgaaagca gctcagtatg acccaaagcc caaaattgat
     8041 gtcggtgatc atctacagct ctatcaaaga agactaaagc accgtgtttt ggacaatatg
     8101 gcaaagcggg caaataccaa tctcgttgcc aaaaggcttc cccataccta tttagctgaa
     8161 gagcaacgca atcagggcag tatctaaagc ggttatcgct cttaactcta gaagcagcga
     8221 ctcctagcat taaatgcacc gcaccttgcg cttggtactc cattaaccga atagcttcgt
     8281 ctcggcgctc cttgcctaca aacggagcat ataaagggaa taaggtatgc tcataaataa
     8341 gctgctgaac agcgtaacgt cctgtttgat gtagatgtct tgctatcaca cctaaatgcg
     8401 agggcagacc taaggtagcg accaccttgc ggttgccata cacctcatcc aacagctgct
     8461 taggactaac aatcccttga taaacgcctg cccgtgcaat agtgctataa atcagctcat
     8521 tcgagtacgg aacaggaaag tttctcatgg ctaacctgct tgtttaaata agctcgccat
     8581 atccactatt acccctttcc cttttaaccg ttcatgcatg gttttttcag gttggcgttg
     8641 tgaatagata taacgtaaat ccgtatcagg caagctatcc cagtcgctgg gcttgactac
     8701 ctttatagcc gaaacctttt tactcttgga gggcttttct agttctgaca ctaccgtttc
     8761 gccttccatc aaccactgca aaacaagagg cagtaacttt tgtcttgtca tcgttggatt
     8821 ctggctaaac gcttttttaa tagtgggaat taacaggctt gaatcgtaat cctctttcag
     8881 catcagatat aaatgacgct gatcttcggt atctaactct tgaagggctt tttcttctgg
     8941 tgtttgttct tgtatcgctg cgatatctag ctgaagttgg attaaccgtt tatcaatctc
     9001 gggaacgact agatcagaat aacgagcaat gcgttctggg atacccgagc gtaatgcctc
     9061 tagcatgggg tgcacaggct ttaactcatc ttgatacact tgccgcaata aaccagcggt
     9121 aatacgctca ttgcctaaag ctagcgcacg gagctgagcg agtacaaaaa gttttactac
     9181 aatgtccatc actccttggc ttagctcata ccacacatca cggacctcat ccgataacag
     9241 cgcatctttg cgttgtaaaa gctgtaattg ccaaagatta tccgtaaaag cgatccactc
     9301 ttgattgggc tttccacgtt gcgtttgttg tataggatcc cagaatatag ctccaaaccc
     9361 tgcccctcta cgtgcagacc gcaaatcagc ctcaaaaatc tctcgtgctt taggggtacc
     9421 aatcaacatc actggtacgc caataatatt caccatcgtc acaaaaaagt tcagcatctc
     9481 ttgagatcca cccgaacgag agcggcttaa atgctgaatt tcatcaataa ccaacaaccc
     9541 taaagcatgt gcattggcta tttgcgacat caaagccaac atggtttcta taccatgacg
     9601 ttttaagcca taacgacgct catagttcga gcccaaggct cgatccaacg ctctgaaaaa
     9661 attcaagcag atttctttta gcgaaccatt atgcgagcag tctattttca aatacaccac
     9721 ctgctctaca ttgagttcac gatggtaaat cacctgagga tacgtggcta gaatacgatg
     9781 aagagaggtc gtcttcccac taccagaaca accaattaac aataagcttt gtgccgtaga
     9841 tcgtgcctcc tcaaagcgaa atgtctccaa ctctcccgtt tgaacacgct cataaccatt
     9901 ttgtaaatgc ttttgtaaat ctcctgtttt aggatttctg cctacgtagc cacctcgaat
     9961 catgaccgaa atacgctcac ttagtagcaa atgcgtacct aatggctgaa aatagtcatc
    10021 tggaatacga caaatggtat gagctctgat aacacgggac ttttgcaagt cagaggaagt
    10081 aagctgtaaa gaggatttca gtgatgcagc actattcact gactcttgta atggtggtaa
    10141 ggcctcgata aaaggattat cacgataagc ctctaccccc gtatcacgat aaactgcttg
    10201 aatccgggta gcactcatga ctcatccttt tctggtggat cctgaaataa ttcaggcacg
    10261 tatgtgggta ggctgtaatc ttcttgatca tccgcttcca ctgcgttgaa aggaataact
    10321 ttagcctcat cacctgaaga gcttggcttc aaatgctccg cacgtttttt acgctccgag
    10381 gtcacggctt cttttttatt agttttaatc tgcttaatgc gtgttgattt gggctcagta
    10441 gtactgggcg ttaacttatt cgctttctga atggtttgct gaataaacgc ctcaagctcc
    10501 ctgcgtttag ttaactcatc ctgcttcgca ttggctttat tgtgtttttc ttgtgcttgt
    10561 atatcccaaa cctcccaaaa tgagagacct ttaaactgcc gactacgttc cgtcagatta
    10621 cagcgccaaa atacacggct gccaacttgc ggaaacaaat aaatcgtatc aaccagcact
    10681 gggtcataag ccgcttctaa atgttgaggt ctagctatat cagtgctccg ctgcaaccaa
    10741 ccctcacgca gaatctctga ccccgagtaa tacaaacccc acaaattaac gccaaatgaa
    10801 gaaatagaga cctttcggcg aggcagtaac gctactcgca actgctcttg ctccacagcc
    10861 cttaaactac ctgtacgatg ctgcataccc cattgccata gctggacagg aatagacggt
    10921 aaatctgtag gaaaatcagc atctcgatcg tatttatcca tcaccagatg gttatttctg
    10981 aataagatcg tacgcaaaat aatttgtgtg aactcaaata ccgacagaga tgcatctaac
    11041 ctatagtctg tttcaccatg gcttttgatc cgactgccct ctacaatgcc aggtgcaaag
    11101 gacttaaact cggcttgtag tgttctaaaa gtgctttcca ctatgccttt agcatcgcca
    11161 cgtctaggtg gagcactttc cactcgcaca ttaaaactag aaactaaggc ttcgacctga
    11221 tgactcatta attcgccacg gtccgctagc aacacatctg gcaaacctac acacggccag
    11281 tctgagctac taatctcaat atcatgctgg gcacaaatgg ccgttttgtc agagcaagca
    11341 tttacaaaag cctgcatcgc caccacataa gacggatttt caaagccgat ataaaagccc
    11401 gtgatcatcc gactaaacac atcaatcaca atgtaaagcg ttggtcttcc tatgattttt
    11461 tggcgatcat gatgatccac taaataaata tcagcaatcg tggcatcaat ctcataacga
    11521 ctcccagggc ctaacgcctg agaagtggct gtactactta agggtcgtac gtctttttta
    11581 tatacccctg ctttaactct agactttaag cgctgagctt tagggtattc tcgatcataa
    11641 aaataacgaa actgacgtag tgttgggtaa tcctcttggg gaatgcgagg aaaatactga
    11701 gcaaacaagt ccacaaatcg tctataggca acggtggtct ttgtaccttt ttgatttaac
    11761 aggtgctttt ctatggtcaa cctaaaaagg cgttcaatct cgggcgttac cttggttcct
    11821 tcacccttac catattctct ggctcggcca atctttgctg ttcctgtcgc tgaacgtctt
    11881 tcccctggtg caccgctgtt tttgtagtca ggaattaatg cattaggcgt ttgaccacgc
    11941 tgccagtaac ggcgtaacaa cttataaact gtagccttag taaccttatg ttcttggacc
    12001 acatgctcaa cgagttcgct tctgacttta gggtcgaaac gatccttact attaataata
    12061 ggaagaattt ttcgataatc ctcctcgcgc ttctggaagc tgacagaatc tacagacggc
    12121 tcttctaaat caagatgtac ataagggtca tcagttctaa ctaagcggcc ttcatctaaa
    12181 tactgcatta gcaacaactc agccctagct tgtggaactc ctttatcagc gcttatttgc
    12241 atccagacaa cttggccatc ctctatagcc aaaatgcgat acggatcatt atcaaatagc
    12301 acaacctcat taatttgcca catagcgcaa ctcctccata ttcactactt ggctaataca
    12361 gagatctgca cacttatttg ccctgaaaga cttataaata ttgaacttaa taaaaccatt
    12421 tgcggttaag gctcgtatct cactcaatgt tttgcctaac tccaaatcat aagcaatatc
    12481 aacctgctta cagacattga taatgttttc atctcctttt tcttgcagga tatgggccaa
    12541 tggggatagt tgtgctaaaa gctccgcaga aacttcttct gttttcactg aataaagcca
    12601 ttcaatattt tcttttacta cgggatttat ttctttatca gtaaaaatga accaaggaat
    12661 ttgcttttgc tgccaatagc gacgctctag ttctagtttt tctaaggtac gctcgtcttg
    12721 taaggctgct gcaggtttga cttgaatagc aaactgctca aaaggaccat ctttgcagtc
    12781 cactaaaaaa tcagtagaca taacctgatc tacaccacga ataacaggat gcttaatacc
    12841 actatctatt gcaatctgcc tggtatcact aggtaataag gggaactgct cgcgtatatc
    12901 tagcacgctg ctctcccact caagactgag aaaaacagca agctctaagt cagatagcaa
    12961 atgatggact cgtcccgtct tatgagaata aatacggtgg gaacgacctg aagaaggaac
    13021 ttcttgtact gttagccatg gaatatagtc tttaccatgc ccttggccac gcccctcttt
    13081 aatacgacgg gcaatttgca cttcagaaaa tgaagagttt gctttagcca taacaaaagt
    13141 ccagtatgct ttttcacagc ataactggac tgatttcagt ttacaactat tctgtctagt
    13201 ttaagacttt attgtcatag tttagatcta ttttgttcag tttaagactt tattgtccgc
    13261 ccacaagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
    13321 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
    13381 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
    13441 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
    13501 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
    13561 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
    13621 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
    13681 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
    13741 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
    13801 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
    13861 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
    13921 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
    13981 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
    14041 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
    14101 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
    14161 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
    14221 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
    14281 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
    14341 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
    14401 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
    14461 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
    14521 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
    14581 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
    14641 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
    14701 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
    14761 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
    14821 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
    14881 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
    14941 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
    15001 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
    15061 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
    15121 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
    15181 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
    15241 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
    15301 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
    15361 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
    15421 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
    15481 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
    15541 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
    15601 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
    15661 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
    15721 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
    15781 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
    15841 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
    15901 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
    15961 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
    16021 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
    16081 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
    16141 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
    16201 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
    16261 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
    16321 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
    16381 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
    16441 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
    16501 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
    16561 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
    16621 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
    16681 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
    16741 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
    16801 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
    16861 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
    16921 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
    16981 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
    17041 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
    17101 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
    17161 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
    17221 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
    17281 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
    17341 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
    17401 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
    17461 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
    17521 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
    17581 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
    17641 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
    17701 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
    17761 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
    17821 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
    17881 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
    17941 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
    18001 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
    18061 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
    18121 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
    18181 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
    18241 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
    18301 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
    18361 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
    18421 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
    18481 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
    18541 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
    18601 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
    18661 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
    18721 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
    18781 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
    18841 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
    18901 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
    18961 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
    19021 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
    19081 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
    19141 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
    19201 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
    19261 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
    19321 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
    19381 tgaacgcttt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
    19441 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
    19501 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
    19561 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
    19621 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
    19681 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
    19741 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
    19801 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
    19861 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
    19921 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
    19981 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
    20041 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
    20101 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
    20161 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
    20221 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
    20281 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
    20341 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
    20401 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
    20461 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
    20521 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
    20581 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
    20641 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
    20701 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
    20761 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
    20821 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
    20881 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
    20941 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
    21001 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
    21061 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
    21121 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
    21181 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
    21241 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
    21301 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
    21361 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
    21421 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
    21481 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
    21541 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
    21601 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
    21661 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
    21721 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
    21781 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
    21841 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
    21901 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
    21961 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
    22021 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
    22081 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
    22141 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
    22201 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
    22261 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
    22321 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
    22381 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
    22441 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
    22501 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
    22561 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
    22621 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
    22681 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
    22741 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
    22801 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
    22861 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
    22921 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
    22981 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
    23041 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
    23101 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
    23161 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
    23221 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    23281 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    23341 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    23401 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    23461 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    23521 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    23581 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    23641 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    23701 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    23761 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    23821 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    23881 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    23941 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    24001 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    24061 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    24121 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    24181 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtctaaaa acagatactg
    24241 ttttaataaa tgacatttac acaacaa
//
LOCUS       DAZSBF010000652        11014 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.137, whole genome shotgun sequence.
ACCESSION   DAZSBF010000652 DAZSBF010000000
VERSION     DAZSBF010000652.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11014
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.137"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(187..537)
                     /locus_tag="ACHXWD_004797"
                     /pseudo
     CDS             complement(187..537)
                     /locus_tag="ACHXWD_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(740..1753)
                     /gene="intI1"
                     /locus_tag="ACHXWD_004798"
     CDS             complement(740..1753)
                     /gene="intI1"
                     /locus_tag="ACHXWD_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFR7623401.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1911..2384
                     /gene="dfrA17"
                     /locus_tag="ACHXWD_004799"
     CDS             1911..2384
                     /gene="dfrA17"
                     /locus_tag="ACHXWD_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA17"
                     /protein_id="HFR7623402.1"
                     /translation="MKISLISAVSENGVIGSGPDIPWSVKGEQLLFKALTYNQWLLVG
                     RKTFDSMGVLPNRKYAVVSKNGISSSNENVLVFPSIENALKELSKVTDHVYVSGGGQI
                     YNSLIEKADIIHLSTVHVEVEGDIKFPIMPENFNLVFEQFFMSNINYTYQIWKKG"
     gene            2515..3303
                     /locus_tag="ACHXWD_004800"
     CDS             2515..3303
                     /locus_tag="ACHXWD_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA5"
                     /protein_id="HFR7623403.1"
                     /translation="MGEFFPAQVFKQLSHARAVIERHLAATLDTIHLFGSAIDGGLKP
                     DSDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGTWRPLELTVVARSEVVPWRYP
                     ARRELQFGEWLRHDILSGTFEPAVLDHDLAILLTKARQHSLALLGPSAATFFEPVPKE
                     HFSKALFDTIAQWNAESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPA
                     EHRPLICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR"
     gene            3509..3856
                     /locus_tag="ACHXWD_004801"
     CDS             3509..3856
                     /locus_tag="ACHXWD_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFR7623404.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            3850..4689
                     /gene="sul1"
                     /locus_tag="ACHXWD_004802"
     CDS             3850..4689
                     /gene="sul1"
                     /locus_tag="ACHXWD_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFR7623405.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            4817..5020
                     /locus_tag="ACHXWD_004803"
     CDS             4817..5020
                     /locus_tag="ACHXWD_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623406.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLGIASENGK"
     gene            5176..6381
                     /gene="chrA"
                     /locus_tag="ACHXWD_004804"
     CDS             5176..6381
                     /gene="chrA"
                     /locus_tag="ACHXWD_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HFR7623407.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRR
                     RWLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILF
                     ALGISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLV
                     PSAWGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVL
                     AELMPSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAV
                     PGPLFTFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQA
                     ALAGINAAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGA
                     AGWLLSVAL"
     gene            6392..6697
                     /locus_tag="ACHXWD_004805"
     CDS             6392..6697
                     /locus_tag="ACHXWD_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFR7623408.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGT
                     VYPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
     gene            6924..7688
                     /locus_tag="ACHXWD_004806"
     CDS             6924..7688
                     /locus_tag="ACHXWD_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HFR7623409.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(8181..8765)
                     /gene="mphR(A)"
                     /locus_tag="ACHXWD_004807"
     CDS             complement(8181..8765)
                     /gene="mphR(A)"
                     /locus_tag="ACHXWD_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFR7623410.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(8765..10003)
                     /gene="mrx(A)"
                     /locus_tag="ACHXWD_004808"
                     /gene_synonym="mrx"
     CDS             complement(8765..10003)
                     /gene="mrx(A)"
                     /locus_tag="ACHXWD_004808"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFR7623411.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(10000..10905)
                     /locus_tag="ACHXWD_004809"
     CDS             complement(10000..10905)
                     /locus_tag="ACHXWD_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFR7623412.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
BASE COUNT         2291 a         3186 c         3248 g         2289 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc cccaatgcgc tgtccagtca
       61 tggaattccc tgcaaaatgt cagggaagac tctatgacct tcaacgagat atgtcaataa
      121 attcaaaatt caatcctatc ctgacgcaat ttacacatgg catctgacat caggttaggg
      181 tatgcctcaa cctgacggcg gcgaatcaca agcgtccggt ttgacgctgg tttcggtcgc
      241 agtgctggcc cgcgcctgga agcgctgata gcactccagc ccgcagaaat gctccacgta
      301 ctcggcccct tccggcgtga aggcggcatc gagcgggatt tccttgcagc acacgcagca
      361 actggtgctc ggttcattgg cgttcatggt ggtgttcctc cattggttga cgaagccgac
      421 gaaggccgcc gccggcatcg gcctggcgaa caggaaaccc tgcaccgtgt cgcaacccgc
      481 ctgccgcaac cacgcaaggc agtcgggtgt ttccacaccc tcggctacca cctccattgt
      541 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
      601 gttggatcca tcaggcaacg acgggctgct gccggccatc agcggacgca gggaggactt
      661 tccgcaaccg gccgttcgat gcggcaccga tggccttcgc gcaggggtag tgaatccgcc
      721 aggattgact tgcgctgccc tacctctcac tagtgagggg cggcagcgca tcaagcggtg
      781 agcgcactcc ggcaccgcca actttcagca catgcgtgta aatcatcgtc gtagagacgt
      841 cggaatggcc gagcagatcc tgcacggttc gaatgtcgta accgctgcgg agcaaggccg
      901 tcgcgaacga gtggcggagg gtgtgcggtg tggcgggctt cgtgatgcct gcttgttcta
      961 cggcacgttt gaaggcgcgc tgaaaggtct ggtcatacat gtgatggcga cgcacgacac
     1021 cgctccgtgg atcggtcgaa tgcgtgtgct gcgcaaaaac ccagaaccac ggccaggaat
     1081 gcccggcgcg cggatacttc cgctcaaggg cgtcgggaag cgcaacgccg ctgcggccct
     1141 cggcctggtc cttcagccac catgcccgtg cacgcgacag ctgctcgcgc aggctgggtg
     1201 ccaagctctc gggtaacatc aaggcccgat ccttggagcc cttgccctcc cgcacgatga
     1261 tcgtgccgtg atcgaaatcc agatccttga cccgcagttg caaaccctca ctgatccgca
     1321 tgcccgttcc atacagaagc tgggcgaaca aacgatgctc gccttccaga aaaccgagga
     1381 tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg ccgcgacggc cgaggtcttc
     1441 cgatctcctg aagccagggc agatccgtgc acagcacctt gccgtagaag aacagcaagg
     1501 ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg ctcgttcgcc agccaggaca
     1561 gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg acgcacaccg tggaaacgga
     1621 tgaaggcacg aacccagtgg acataagcct gttcggttcg taaactgtaa tgcaagtagc
     1681 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1741 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
     1801 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     1861 cgcagcaggg cagtcgccct aaaacaaagt tagccattaa gggagttaaa ttgaaaatat
     1921 cattgatttc tgcagtgtca gaaaatggcg taatcggtag tggtcctgat atcccgtggt
     1981 cagtaaaagg tgagcaacta ctctttaaag cgctcacata taatcaatgg ctccttgtcg
     2041 gaagaaaaac atttgactct atgggtgttc ttccaaatcg caaatatgca gtagtgtcaa
     2101 agaacggaat ttcaagctca aatgaaaacg tcctagtttt tccttcaata gaaaatgctt
     2161 tgaaagagct atcaaaagtt acagatcatg tatatgtctc tggcgggggt caaatctata
     2221 atagccttat tgaaaaagca gatataattc atttgtctac tgttcacgtt gaagtcgaag
     2281 gtgatatcaa attccctata atgcctgaga atttcaattt ggtttttgaa cagtttttta
     2341 tgtctaatat aaattataca taccagattt ggaaaaaagg ctaacaatgc gttgcagcac
     2401 cagtcgcttc gctccttgga cagcttttaa gtcgcgtctt tgtggttttg ctgcgcaaaa
     2461 gtattccaca aagccgcaac ttaaaagctg ccgctgaact taacgttagg catcatgggt
     2521 gaatttttcc ctgcacaagt tttcaagcag ctgtcccacg ctcgcgcggt gatcgagcgc
     2581 catctggctg cgacactgga cacaatccac ctgttcggat ctgcgatcga tggagggctg
     2641 aagccggaca gcgacataga cttgctcgtg accgtcagcg ccgcacctaa cgattcgctc
     2701 cggcaggcgc taatgctcga tttgctgaaa gtctcatcac cgccaggcga tggcggaaca
     2761 tggcgaccgc tggagctaac tgttgtcgct cgaagcgaag tagtgccttg gcgctatccg
     2821 gcgcggcgtg agcttcagtt cggtgagtgg ctccgccacg acatcctttc cggaacgttc
     2881 gagcctgccg ttctggatca cgatcttgcg attttgctga ccaaggcgag gcaacacagc
     2941 cttgcgcttc taggcccatc cgcagccacg tttttcgagc cggtgccgaa ggagcatttc
     3001 tccaaggcgc ttttcgacac tattgcccag tggaatgcag agtcggattg gaagggtgac
     3061 gagcggaacg tcgttcttgc tcttgctcgc atttggtaca gcgcttcaac tggtctcatt
     3121 gctcctaagg acgttgctgc cgcatgggta tcggagcgtt tgcctgccga gcatcggccc
     3181 ctcatctgca aggcacgcgc ggcgtacctg ggtagcgagg acgacgacct agcaatgcgc
     3241 gtcgaagaga cggccgcgtt cgttcgatat gccaaagcaa cgattgagag aatcttgcgt
     3301 tgagcggcat gtgcgaaaag tgcatcgacc cgcgccgagg gcatctgatg cctaactcgg
     3361 cgttcaagcg gacgggctgc gcccgccgct caactatgcg ttagatgcac taagcacata
     3421 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     3481 aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg ttatcgcaat
     3541 agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta ctaagcttgc
     3601 cccttccgcc gttgtcataa tcggttatgg catcgcattt tattttcttt ctctggttct
     3661 gaaatccatc cctgtcggtg ttgcttatgc agtctggtcg ggactcggcg tcgtcataat
     3721 tacagccatt gcctggttgc ttcatgggca aaagcttgat gcgtggggct ttgtaggtat
     3781 ggggctcata attgctgcct ttttgctcgc ccgatcccca tcgtggaagt cgctgcggag
     3841 gccgacgcca tggtgacggt gttcggcatt ctgaatctca ccgaggactc cttcttcgat
     3901 gagagccggc ggctagaccc cgccggcgct gtcaccgcgg cgatcgaaat gctgcgagtc
     3961 ggatcagacg tcgtggatgt cggaccggcc gccagccatc cggacgcgag gcctgtatcg
     4021 ccggccgatg agatcagacg tattgcgccg ctcttagacg ccctgtccga tcagatgcac
     4081 cgtgtttcaa tcgacagctt ccaaccggaa acccagcgct atgcgctcaa gcgcggcgtg
     4141 ggctacctga acgatatcca aggatttcct gaccctgcgc tctatcccga tattgctgag
     4201 gcggactgca ggctggtggt tatgcactca gcgcagcggg atggcatcgc cacccgcacc
     4261 ggtcaccttc gacccgaaga cgcgctcgac gagattgtgc ggttcttcga ggcgcgggtt
     4321 tccgccttgc gacggagcgg ggtcgctgcc gaccggctca tcctcgatcc ggggatggga
     4381 tttttcttga gccccgcacc ggaaacatcg ctgcacgtgc tgtcgaacct tcaaaagctg
     4441 aagtcggcgt tggggcttcc gctattggtc tcggtgtcgc ggaaatcctt cttgggcgcc
     4501 accgttggcc ttcctgtaaa ggatctgggt ccagcgagcc ttgcggcgga acttcacgcg
     4561 atcggcaatg gcgctgacta cgtccgcacc cacgcgcctg gagatctgcg aagcgcaatc
     4621 accttctcgg aaaccctcgc gaaatttcgc agtcgcgacg ccagagaccg agggttagat
     4681 catgcctagc attcaccttc cggccgcccg ctagcggacc ctggtcaggt tccgcgaagg
     4741 tgggcgcaga catgctgggc tcgtcaggat caaactgcac tatgaggcgg cggttcatac
     4801 cgcgccaggg gagcgaatgg acagcgagga gcctccgaac gttcgggtcg cctgctcggg
     4861 tgatatcgac gaggttgtgc ggctgatgca cgacgctgcg gcgtggatgt ccgccaaggg
     4921 aacgcccgcc tgggacgtcg cgcggatcga ccggacattc gcggagacct tcgtcctgag
     4981 atccgagctc ctagggatcg cctcagaaaa cggaaaataa agcacgctaa gccgtaagta
     5041 agcgtgctcc tgtgaaagcc acagctaaaa ctgcgtagta cacatagagg tatttttttc
     5101 tagatttata tcgaatttcg ttatcgaatt tcgcttatta ttgatcaact cgttgcagat
     5161 catgaaggcg aaagtatgac gaataaccct accgatgaca gcagaccatg gtcggtcttt
     5221 cttatttttc tgcggcttgg attgacatct tttggcggcc ccattgcgca cttgggctac
     5281 ttccgcgccg aatttgtcac acggcggcgc tggctctccg aacggagcta tgctgacttg
     5341 gtcgcgcttt gtcagttctt gccagggcct gcaagcagcc aggtcggcat agcggtagga
     5401 ctgtctcggg ctggatacag cggggcgctg gctgcttggg ctggcttcac gctgccgtct
     5461 gccatagcct tgatcctttt tgcgctcggc atctccagct atggcgatta cgtctcgcag
     5521 ggcgcgttgc atggcttaaa agtggtggct gtggccgtgg tcgctcaagc agtatggggc
     5581 atggcgcgta acctatgcac ggatgggctg cgagtcacca tcatggcaat tgctacctgc
     5641 gtcgttttac ttgtgccgtc cgcgtgggga caggttggcg tgattgctat cgcaggcatc
     5701 gcaggccggt tattgttcaa gccagcgaaa gttgttgagc atgaccccct acctatcacg
     5761 gtcagtcacc gggccggcgt gctttggctc tcgctgttct ttgtcttgct gattggcctg
     5821 ccggtgttgg ccgaactgat gccaagtcaa accatggcaa tggtggattc cttctatcgt
     5881 gtcggatcac tggtgttcgg cggtggtcac gttgtgctgc cattactgca agccgaagtg
     5941 gtgccctccg gctgggtcaa caatgaatcc tttctcgcgg ggtacggggc agctcaagcg
     6001 gtgcccggcc ctttgttcac gttcgccgcg tttcttggtg cctcgatgaa caccgccccg
     6061 tcgggctgga tcggcggcat tgtgtgtctg ctggctatct tcgcgccctc gttcttgctg
     6121 gtcgtcggat caatgccatt ttgggagcgt ttgcgccgca atacaggcat ccaagctgcg
     6181 ctggccggga tcaatgccgc tgtagtcggc ttgctgctgg ccgcgctgta tcagcctgta
     6241 tggactagcg ccatctttca gccgcaagac ttcggcttgg cattagttgc ccttgtcgca
     6301 cttatgttct ggaagctccc gccgtggctg gttgtcctcg gtagcggtgc agctgggtgg
     6361 ctgttgagcg tcgctctgtg aggcacaaaa aatgactgac aaagacctct acggcggttt
     6421 gatccgcctg cacatccttc accatgcagc cgaggaacct gtctttgggc tggggatcat
     6481 cgaagagcta cgccgacacg gctacgagat gagcgctggc accgtgtacc cgatgctgca
     6541 cggcctggaa aaaaaaggct atctgacctc acgccacgaa cgcaccgggc gacgtgaacg
     6601 gcgtgtttat gacatcactg aacaaggcag aactgcactt gctgatgcga aaacaaaggt
     6661 caaggagctg ttcggagagt tggtagaagg tggttgagtt tttgcttcta tgtcaggttg
     6721 agctataccc taactggatt gtcattttca gaagacgact gcaccagttg attgggcgta
     6781 atggctgttg tgcagccagc tcctgacagt tcaatatcag aagtgatctg caccaatctc
     6841 gactatgctc aatactcgtg tgggctctgt tgcaaaaatc gtgaagcttg agcatgcttg
     6901 gcggagattg gacggacgga acgatgacgg atttcaagtg gcgccatttc cagggtgatg
     6961 tgatcctgtg ggcggtgcgc tggtattgtc gctatccgat cagctatcgc gaccttgagg
     7021 aaatgctggc ggaacgcggc atttcggtcg accatacgac gatctatcgc tgggtccagt
     7081 gctacgcccc ggagatggag aagcggctgc gctggttctg gcggcgtggc tttgatccga
     7141 gctggcgcct ggatgaaacc tacgtcaagg tgcggggcaa gtggacctac ctgtaccggg
     7201 cagtcgacaa gcggggcgac acgatcgatt tctacctgtc gccgacccgc agcgccaagg
     7261 cagcgaagcg gttcctgggc aaggccctgc gaggcctgaa gcactgggaa aagcctgcca
     7321 cgctcaatac cgacaaagcg ccgagctatg gtgcagcgat caccgaattg aagcgcgaag
     7381 gaaagctgga ccgggagacg gcccaccggc aggtgaagta tctcaataac gtgatcgagg
     7441 ccgatcacgg aaagctcaag atactgatca agccggtgcg cggtttcaaa tcgatcccca
     7501 cggcctatgc cacgatcaag ggattcgaag tcatgcgagc cctgcgcaaa ggacaggctc
     7561 gcccctggtg cctgcagccc ggcatcaggg gcgaggtgcg ccttgtggag agagcttttg
     7621 gcattgggcc ctcggcgctg acggaggcca tgggcatgct caaccaccat ttcgcagcag
     7681 ccgcctgatc ggcgcagagc gacagcctac ctctgactgc cgccaatctt tgcaacagag
     7741 cccgccgtgc tagtctgctc ggtgatggtg gagtgaagcc aacccgcaat cgggttatga
     7801 atctgcatcg cgattcgcaa tcagctgtct cttgagcatg tcgaactcct gcgatgttat
     7861 ctcgccgtgc tcgtgctgct acaccaactt gtccaactca tccgccacac cgctccccac
     7921 cacctgcgat ggccaggttg tttgctgggc gcattggcgg agtatcgtct cgacccggga
     7981 cacccacacc ggcacaacct tacgggagtg attcactgtc aaagaatcgg cccggtgctc
     8041 tgacgcaagt atcgggatgg tcaccatttg taagccgtag acctgagtgg tgatcaagac
     8101 ttcgatacca ccgaccgtac cggtactaat cgacgacggt cgtgttcgtc gcctgccgca
     8161 gggactctgc acacctccgt ttacgcatgt gcctggagga gttggaaatc gtcgtgttcg
     8221 ggaaacatta aacacaggat ggcagcgatc tgagccagca catgatcagc tagctcacca
     8281 tccggatcga cggcccactg catcgtcgcg ccagcgatga ccgagtgcag gagcaactca
     8341 gctgccgcag gagcacctgg gggcagtcgc ttgcggatcc cctccaccac cgcgcggttc
     8401 cgctggatcg caagcgtgcg tagctccggc acctggagct cgtaccagga gatgagatag
     8461 ttcaccgaga agtcgttgcg agtgttcatg ctccgaacga gcacctgcaa aaattcccag
     8521 agcccttgcg gccctgcgcc tatcggtatc gcattcaggt aatgccgcac ctgctcgacg
     8581 ccgcgctcca tcatcctcac cagcagcgta tcgcggttgg tgaagcgctg gattaacgct
     8641 gcgcgggaga gccccacctc ctttgctact ccgctgagcg tgaactctat gggaccgcaa
     8701 cgcttcagca ctacggtggc ggcctcgagt acctcgtcat cggacttgag cttggggcgg
     8761 ggcatcagtg ttcaccttct gtatgggttg gggcggaggc tgtggctgcc gccgccattg
     8821 tagcaaattg aagacggagc gagagtagag ccacgagccc cgcaaacacg gccgatacaa
     8881 cgaggccagg gagcggacca gcaaggtcga caaacgcgcc ggccgcaagc ataaccatgg
     8941 gcgaggctga cagcatcacc gccgagaccg tgccgagtac ccggccgaga agttctggcg
     9001 gcgtgcggtt gtagatggca gcgttgagaa tgggagagac tgagccggtc agcagtccca
     9061 cgagcgcgcc caacaacatc agcaccggca cgcctggcaa ctgtgaaagc agaagcgagc
     9121 ccaccgcaga gccacaaaat gccaccgcca gccagttctg cgctgatatc cgggcgccga
     9181 ccgacgcatg aatggcaatg ccaaggagac caccagcccc catcattgag gagaacagcc
     9241 cgagctctgc tacttggcgt cctgcatcta caaacagcgc aggcatgatg acgctgccgt
     9301 tggcgccaac gatgcccacg aagatcatca ctataccaaa gagagggcgc agcaggggtt
     9361 cgctccagag aaaagcgacg ccggcgcgca tggagagagt cgccgtcgtg gtcatcgtcc
     9421 gagcggcacg cgcgggaagc acccacgcgc cgagcagacc tgcaaggacg gagcagaacg
     9481 ccgtcagccc gagcgttggc gcagcgccaa gcaggccgat tgcggccccc ccaagggccg
     9541 ggccacctag aatcgcgacg ttcccgatca ccgctttcag tgacgagacg cgctcaacgg
     9601 agagcccggc gacgtggccg agtttgggca gctcactgtc ctgcgcggcc ataccgggtg
     9661 cgtcgaacgc ggcaccgagc accacgcaag cgatcagccc agtgttcgag agggcgccaa
     9721 cggcatcgag cagtgggatg ctcgccatgg ccacgccgcc caccacaccc gagatcaatg
     9781 cgacgggcgc gcgcccgaac cgatcgacga ggccaccacc aacccacgcg ccgatgatgg
     9841 tcgcgatgac gctgctagcg gccgtggcgc ccgcccaggc cgcgctcttt gtatgagaca
     9901 ggacgaacca tggaagcgcg agggccgcca ccgcgttgcc gatccggaag agaaaggtcg
     9961 ccgcgaacag cgtcgcgagc gggctatatc gacgttcgct cattccgctg cggcgagctg
    10021 cgccttcgcc gcagcgaggt actcttcgtt acccgagtcg agggcgaaga gtgcgtaggt
    10081 gaccgccccg aacgcaaggc gctccgcgat gtggtgggcg agccgcggcc acacccggcc
    10141 accggccgct tcatacgtga ggaggagctt cgcgagcccc tcttcaccaa agaccataag
    10201 gtgcgcggcc atgtcgatgg cagggtcatc aacgcgggcc tcgctccagt cgatcatccc
    10261 gctgacgcgc tccgtgttgt cgatgagcac atggcccacg tagagatcgc catgcaccac
    10321 cacggagaaa tctggccacg acgaatcgtc gtcgagccag cgctgccacc ggtggaggcg
    10381 cttgtcgttc accacgaact cgcgtcggac gcggtcaacg tcgtcggcca ccttctgacg
    10441 ggcctgcgtc ggtgtacgga tgagcatccc cgcatccacg gcggcggaaa tggggacggc
    10501 atgcagggcg gcgagcgcgg tcgcgaagct ctccgcgaag acctccgagt cctgcggcac
    10561 gacccagtcg ggcgtggacg aaccaggctg gatgaccatc gcagtcgagt cttcgagcat
    10621 gggataggca acgagctcgg cgttggccac gcgccagtcc ggcaccgcga acggcaggcg
    10681 attcttgagc attgccagca cccgcgcctc tggttcgacc ttcgcgctta cctcggctcg
    10741 gcgcgggatg cgcagcaccc accgacgtcc atcgtcgacg gtggcgatca cgatcctata
    10801 gtcgagccca agctcattga cagtcagcgg gccatggagc ttgagcccat gtcgggctgc
    10861 aagtgcgtac agttgggagg tatcggcggt cgtgactacg gtcatgattc actcctgagg
    10921 gcttgacggg tttagccacc taaatgtaac agtcacgtcg gttatattca atccggcact
    10981 gttgcaaagt tagcgatgag gcagcctttt gtct
//
LOCUS       DAZSBF010000653         9703 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.154, whole genome shotgun sequence.
ACCESSION   DAZSBF010000653 DAZSBF010000000
VERSION     DAZSBF010000653.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.154"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHXWD_004810"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHXWD_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHXWD_004811"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHXWD_004811"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR7623413.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHXWD_004812"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHXWD_004812"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR7623414.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHXWD_004813"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHXWD_004813"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR7623415.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHXWD_004814"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHXWD_004814"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR7623416.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHXWD_004815"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHXWD_004815"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR7623417.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHXWD_004816"
     CDS             5099..6232
                     /locus_tag="ACHXWD_004816"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR7623418.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHXWD_004817"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHXWD_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR7623419.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHXWD_004818"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHXWD_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR7623420.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHXWD_004819"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHXWD_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR7623421.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHXWD_004820"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHXWD_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR7623422.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHXWD_004821"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHXWD_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR7623423.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHXWD_004822"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHXWD_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR7623424.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2467 a         2486 c         2475 g         2275 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggt ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccatccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatata gagtgagtac ggc
//
LOCUS       DAZSBF010000654         7426 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.208, whole genome shotgun sequence.
ACCESSION   DAZSBF010000654 DAZSBF010000000
VERSION     DAZSBF010000654.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.208"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            99..257
                     /locus_tag="ACHXWD_004823"
     CDS             99..257
                     /locus_tag="ACHXWD_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR7623425.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            717..1532
                     /gene="sul2"
                     /locus_tag="ACHXWD_004824"
     CDS             717..1532
                     /gene="sul2"
                     /locus_tag="ACHXWD_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR7623426.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1593..2396
                     /locus_tag="ACHXWD_004825"
                     /note="aph(3'')-Ib"
     CDS             1593..2396
                     /locus_tag="ACHXWD_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFR7623427.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCQFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2396..3232
                     /locus_tag="ACHXWD_004826"
     CDS             2396..3232
                     /locus_tag="ACHXWD_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HFR7623428.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            3538..3780
                     /locus_tag="ACHXWD_004827"
     CDS             3538..3780
                     /locus_tag="ACHXWD_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFR7623429.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(3812..4462)
                     /gene="tetR(A)"
                     /locus_tag="ACHXWD_004828"
     CDS             complement(3812..4462)
                     /gene="tetR(A)"
                     /locus_tag="ACHXWD_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFR7623430.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4568..5767
                     /gene="tet(A)"
                     /locus_tag="ACHXWD_004829"
     CDS             4568..5767
                     /gene="tet(A)"
                     /locus_tag="ACHXWD_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFR7623431.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWRN
                     SSNSRCT"
     gene            complement(5734..5922)
                     /locus_tag="ACHXWD_004830"
                     /pseudo
     CDS             complement(5734..5922)
                     /locus_tag="ACHXWD_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(5966..6355)
                     /locus_tag="ACHXWD_004831"
     CDS             complement(5966..6355)
                     /locus_tag="ACHXWD_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623432.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            6573..7013
                     /locus_tag="ACHXWD_004832"
     CDS             6573..7013
                     /locus_tag="ACHXWD_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR7623433.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(7072..>7426)
                     /locus_tag="ACHXWD_004833"
     CDS             complement(7072..>7426)
                     /locus_tag="ACHXWD_004833"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623434.1"
                     /translation="TSLLHHLYITFTSFLQLIRHFHPLCHLYITPFLNVLTAGFPAAP
                     ATLHGHAINCVPVHRFRMGRTPETGLTPAEWCEFPRYALVRILMHAVKTEPARFWRVF
                     GWFVACFTGFPSETP"
BASE COUNT         1580 a         2098 c         2082 g         1666 t
ORIGIN      
        1 ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt tctcattcca cgcctgacac
       61 tcagttccgt gaaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
      121 ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa agacacgaca
      181 aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga ttaagaggtc
      241 ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat ggcggtctcg
      301 tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa caaaccgcct
      361 ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa aggatctcaa
      421 gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag tcactgttga
      481 ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca gcaccaaaga
      541 agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc caaaatgcta
      601 cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg gattcccttt
      661 cgacccgagc atccgtatga gactcatgct cgattattat tattatagaa gcccccatga
      721 ataaatcgct catcattttc ggcatcgtca acataacctc ggacagtttc tccgatggag
      781 gccggtatct ggcgccagac gcagccattg cgcaggcgcg taagctgatg gccgaggggg
      841 cagatgtgat cgacctcggt ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg
      901 acacagaaat cgcgcgtatc gcgccggtgc tggacgcgct caaggcagat ggcattcccg
      961 tctcgctcga cagttatcaa cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct
     1021 atctcaatga tattcgcggt tttccagacg ctgcgttcta tccgcaattg gcgaaatcat
     1081 ctgccaaact cgtcgttatg cattcggtgc aagacgggca ggcagatcgg cgcgaggcac
     1141 ccgctggcga catcatggat cacattgcgg cgttctttga cgcgcgcatc gcggcgctga
     1201 cgggtgccgg tatcaaacgc aaccgccttg tccttgatcc cggcatgggg ttttttctgg
     1261 gggctgctcc cgaaacctcg ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct
     1321 tcgatttgcc ggtgcttctg tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc
     1381 gtggtccggg ggatgtcggg gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg
     1441 gagctgactt catccgcaca cacgagccgc gccccttgcg cgacgggctg gcggtattgg
     1501 cggcgctgaa agaaaccgca agaattcgtt aactgcacat tcgggatatt tctctatatt
     1561 cgcgcttcat cagaaaactg aaggaacctc cattgaatcg aactaatatt ttttttggtg
     1621 aatcgcattc tgactggttg cctgtcagag gcggagaatc tggtgatttt gtttttcgac
     1681 gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg
     1741 gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca
     1801 actggcagga ggaacaggag ggtgcatgct tggtgataac ggcaattccg ggagtaccgg
     1861 cggctgatct gtctggagcg gatttgctca aagcgtggcc gtcaatgggg cagcaacttg
     1921 gcgctgttca cagcctatcg gttgatcaat gtcagtttga gcgcaggctg tcgcgaatgt
     1981 tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg
     2041 aggacaagag tacgccgcag ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc
     2101 ggctcgacca agagcgcacc gatatggttg tttgccatgg tgatccctgc atgccgaact
     2161 tcatggtgga ccctaaaact cttcaatgca cgggtctgat cgaccttggg cggctcggaa
     2221 cagcagatcg ctatgccgat ttggcactca tgattgctaa cgccgaagag aactgggcag
     2281 cgccagatga agcagagcgc gccttcgctg tcctattcaa tgtattgggg atcgaagccc
     2341 ccgaccgcga acgccttgcc ttctatctgc gattggaccc tctgacttgg ggttgatgtt
     2401 catgccgcct gtttttcctg ctcattggca cgtttcgcaa cctgttctca ttgcggacac
     2461 cttttccagc ctcgtttgga aagtttcatt gccagacggg actcctgcaa tcgtcaaggg
     2521 attgaaacct atagaagaca ttgctgatga actgcgcggg gccgactatc tggtatggcg
     2581 caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata
     2641 tgccggggag cgaatgctct ctcacatcgt tgccgagcac ggcgactacc aggcgaccga
     2701 aattgcagcg gaactaatgg cgaagctgta tgccgcatct gaggaacccc tgccttctgc
     2761 ccttctcccg atccgggatc gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa
     2821 cgcaggttgt caaactgact acgtccacgc ggcgattata gccgatcaaa tgatgagcaa
     2881 tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat gaaaacatca tgttctccag
     2941 tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc
     3001 caatatgttc tacgatccgg ctgacagaga cgacctttgt ctcgatccta gacgcattgc
     3061 acagatggcg gacgcattct ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca
     3121 ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg
     3181 cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact agatatcaag
     3241 cgacttctcc tatcccctgg gaacacatca atctcaccgg agaatatcgc tggccaaagc
     3301 cttagcgtag gattccgccc cttcccgcaa acgacccctg gaagccccgt agcgacgcgg
     3361 agaggggcga gacaagccaa gggcgcaggc tcgatgcgca gcacgacata gccggttctc
     3421 gcaaggacga gaatttccct gcggtgcccc tcaagtgtca atgaaagttt ccaacgcgag
     3481 ccattcgcga gagccttgag tccacgctag atctatctca tctgcgcaag gcagaacgtg
     3541 aagacggccg ccctggacct cgcccgcgag cgccaggcgc acgaggccgg cgcgcggacc
     3601 cgcgccacgg cccacgagcg gacgccgcag caggagcgcc agaaggccgc cagagaggcc
     3661 gagcgcggcc gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agcgggctgc
     3721 tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag
     3781 tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa
     3841 cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct
     3901 cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg
     3961 agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct
     4021 cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc
     4081 cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg
     4141 gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct
     4201 gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca
     4261 ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct
     4321 tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc
     4381 gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg
     4441 tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac
     4501 caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag
     4561 gccagacgtg aaacccaaca gacccctgat cgtaattctg agcactgtcg cgctcgacgc
     4621 tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc
     4681 gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttga tgcaatttgc
     4741 ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccgg tcttgctcgt
     4801 ctcgctggcc ggcgctgctg tcgactacgc catcatggcg acggcgcctt tcctttgggt
     4861 tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc
     4921 ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc
     4981 ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc
     5041 cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg
     5101 tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa
     5161 cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt
     5221 cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga
     5281 ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct
     5341 gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg
     5401 ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac
     5461 acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc
     5521 ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg
     5581 ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat
     5641 ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta
     5701 cttgctctgc ctgccggcgc tgcgtcgcgg gctttggaga aattcttcaa attcccgttg
     5761 cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata
     5821 ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt
     5881 atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat
     5941 actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc
     6001 ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cactcttaca ttgttcgcag
     6061 tatctaggta catgttattg cgtgcacctt catgatgtat aatgttatta atgacagcag
     6121 aaagatcgac agaattaata ttcttcgtac ctgggacagt tatcgggtgt cggccagtga
     6181 tgagaccaaa ggatgcagta taagatgaac cactactatt atcatggcca ccattaccac
     6241 caccgttaaa actacctccc gaacttccag agccgctatg accatcattg taggggtcat
     6301 ttggtcccat gtgaccaaaa ccaccgacag taccgctatt taactctgta ctcataaaat
     6361 tatcctcttt gacttttaaa aacagcaaaa actgtattta taacaatgtg tttatataca
     6421 gtattcgaaa tactacgccc gtcaaaacca caccgtcaac aggttttttg tgtatttttc
     6481 acgcctcacg ggcagcctca gaccttttgt gttaaaacag taatatgttc tgctgttaca
     6541 atacggtaca cacaggagac actgtcactt ttatggcatg gcacacagtt caagagacac
     6601 tgaaattaac aggaaagtcg cgctcgcaat tatatcgtga catggctaaa ggccttgtca
     6661 gctatcgcac aggaaaaaat gatcgccggg agttcgagac cagtgaactg attcgggcct
     6721 atggcgagct gcgcacttct gagacaccaa aaaggcacaa ggagggacac tccaaatctg
     6781 tcgttagcca ggcggaaaat gctcagctag acgtcattca gcagaaactg gacgctctgc
     6841 agcagactgt taccctaatg cttgaagaca agatggcgcg ggaagcagaa aaccgcctgc
     6901 gaaatgagga gagggaacaa ctacaggccg aaattagccg tctggctgac gcgctggcac
     6961 aggaaaaaaa gcggggattc tggtcgcggc tgtttcgctc cggttcagag taacagtgca
     7021 ggtgactctg atttaccata acatctgtta tcagcaccgc ctgagaggcc ctcagggcgt
     7081 ttctgacggg aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc
     7141 aggctctgtt tttacggcat gcatgagtat gcgcacaagt gcatagcgcg gaaattcaca
     7201 ccattcagcc ggggtaagtc ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg
     7261 gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca gccggaaaac ccgctgtcag
     7321 cacgttcagg aagggggtga tgtacaaatg gcacagagga tgaaagtgac gtatgagctg
     7381 aaggaatgat gtaaaagtga tgtataagtg atgtaaaagt gatgta
//
LOCUS       DAZSBF010000655         6443 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.230, whole genome shotgun sequence.
ACCESSION   DAZSBF010000655 DAZSBF010000000
VERSION     DAZSBF010000655.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.230"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHXWD_004834"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHXWD_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHXWD_004835"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHXWD_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR7623435.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHXWD_004836"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHXWD_004836"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR7623436.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHXWD_004837"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHXWD_004837"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR7623437.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHXWD_004838"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHXWD_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR7623438.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHXWD_004839"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHXWD_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR7623439.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHXWD_004840"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHXWD_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR7623440.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1570 a         1586 c         1651 g         1636 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatagctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aac
//
LOCUS       DAZSBF010000656         2137 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.387, whole genome shotgun sequence.
ACCESSION   DAZSBF010000656 DAZSBF010000000
VERSION     DAZSBF010000656.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.387"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..290
                     /locus_tag="ACHXWD_004841"
     CDS             <1..290
                     /locus_tag="ACHXWD_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR7623441.1"
                     /translation="RKTALQGGFFVYRARDYDATERISRRSFTVMEIYPIRAHRIHIV
                     ITLDLREFQQQQEKDFLQTSLQQAKFNQKKAAELLGLTYHQLRALLKKHQI"
     gene            384..1028
                     /locus_tag="ACHXWD_004842"
     CDS             384..1028
                     /locus_tag="ACHXWD_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="HFR7623442.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQ
                     FYDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFM
                     NMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGE
                     FSTFDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
     gene            complement(1410..1646)
                     /locus_tag="ACHXWD_004843"
     CDS             complement(1410..1646)
                     /locus_tag="ACHXWD_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR7623443.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARA
                     QIELQRYADRQRALREDERQQSDFAWSRPRAPRR"
BASE COUNT          552 a          520 c          551 g          514 t
ORIGIN      
        1 ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag agcaagagat tacgacgcca
       61 cagaaaggat ctcaagaaga tccttcaccg taatggaaat ctatccaatc cgagcacata
      121 ggatacacat cgttataacg ctggatctgc gtgaattcca gcagcagcaa gagaaagact
      181 ttctacaaac cagcttacag caggctaaat ttaaccagaa aaaagccgct gaattactgg
      241 gtctgactta tcatcagcta cgtgccttgc tgaagaaaca ccagatctaa tacgcatttg
      301 caggtgtcat actggcgggt ttgacgcata acgtcataag gtttaccatg gcgtcattac
      361 tgtataaaaa cacaggcata gatatgactc tggcattagt tggcgaaaaa attgacagaa
      421 atcgcttcac cggtgagaaa gttgaaaata gtacattttt taactgcgat ttttcaggtg
      481 ccgacctgag cggcactgaa tttatcggct gccagttcta tgatcgcgaa agtcagaaag
      541 ggtgcaattt tagtcgcgca atgctgaaag atgccatttt caaaagctgt gatttatcaa
      601 tggcagattt ccgcaacgtc agtgccttgg gcattgaaat tcgccactgc cgcgcacaag
      661 gcgcagattt ccgcggtgca agctttatga atatgatcac cacgcgcacc tggttttgca
      721 gcgcatatat cactaatact aatctaagct acgccaattt ttcgaaagtc gtgttggaaa
      781 agtgtgagct gtgggaaaac cgctggatgg ggactcaggt actgggtgcg acgttcagtg
      841 gttcagatct ctccggcggc gagttttcga ctttcgactg gcgagcagca aacttcacac
      901 attgcgatct gaccaattcg gagttaggtg acttagatat tcggggtgtt gatttacaag
      961 gcgttaagtt agacagctac caggcatcgt tgctcatgga gcggcttggc atcgctgtga
     1021 ttggttagtt cagcgtttac accgggagat gcgtttgacg atttactttt acccgcgatc
     1081 atcgcgttta ttgagtttat tatttatcta tgcgattccg atgaaaaatt cgcacgaaaa
     1141 tatggctaac tcatagcaaa aaaaaagccc cctaagggct tttttttgta cctgacggtc
     1201 agaccgatta aacataacat cccttgcccg cacccccgca ggcgccctca gcgcaatcct
     1261 gctgcatcag ggccgtttcc agcacctttc ctcaccagta tcccggaaaa acgcccctga
     1321 tgatttctgc gcgactgcat ggctatgcac gcaggtgaat ggcaggtgat ttcgcagcgt
     1381 cagcaccact aaaccgccaa aagccaaaat cagcggcgcg gcgcgcgcgg acgtgaccag
     1441 gcaaaatcgg actgctgccg ctcatcctca cgtaatgcac gctggcggtc ggcgtaacgc
     1501 tgcaactcaa tttgcgcacg cgctctgttc aggacgatgt gccggttagg ctgatttagc
     1561 tttttacctg acgctctttc agctgcggca acagcatcat caagaaattt tttctctgca
     1621 ggcgtcaagt ttgtcagtct ggccataacg ttctccagtc agaataaatc agcacagtct
     1681 gccagacaca gtagaaacga tgaagcgcca gcgacggtga aaggcagtca gaaacggtga
     1741 acgggagcgg attttaatcc ggtgccgtga ggcgttggac aagccgcagg cgcgtcatta
     1801 gcttttagcc ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat
     1861 actggctgac tatgttggca cggatgtgga aatcagtgaa gtgcttcatg tagcaggaga
     1921 aaaaaggcgg caccagcgcg tcagcaggat atgtagtaca ggatatattc cgcttcctcg
     1981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtgccggc ttacaggcgg
     2041 ggcggaaatt tcctgggaga taccaggaag gagcttaaca gggaagagat aaggccgcgg
     2101 cgaagccgtt tttccatagg ctccgccccc ctgacaa
//
LOCUS       DAZSBF010000657         1734 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.413, whole genome shotgun sequence.
ACCESSION   DAZSBF010000657 DAZSBF010000000
VERSION     DAZSBF010000657.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.413"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..474
                     /locus_tag="ACHXWD_004844"
     CDS             <1..474
                     /locus_tag="ACHXWD_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR7623444.1"
                     /translation="DDKFITTDYLQQCPTDREGLDLLRMKVEEGDVILVKKLDRLGRD
                     TADMIQLIKEFDAQGVAVRFIDDGISTDGDMGQMVVTILSAVAQAERRRILERTNEGR
                     QEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATEIAHQLSIARSTVYKILEDERAS"
     gene            657..1517
                     /locus_tag="ACHXWD_004845"
     CDS             657..1517
                     /locus_tag="ACHXWD_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFR7623445.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1667..1734)
                     /locus_tag="ACHXWD_004846"
                     /pseudo
     CDS             complement(1667..1734)
                     /locus_tag="ACHXWD_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          462 a          386 c          443 g          443 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc caacagaccg ggaagggctg
       61 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
      121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
      181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
      241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
      301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
      361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
      421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
      481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      601 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
      661 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
      721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
      781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
      841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
      901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1021 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     1081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     1381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     1441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     1501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     1561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1621 aaatccctta acgtgagttt tcgttccact gagcgtcaga cccctgagag cagagatagc
     1681 gctgatgtcc ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaac
//
LOCUS       DAZSBF010000658         1318 bp    DNA     linear   BCT 25-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2002259
            SAMN43277484-rid23495443.guided.464, whole genome shotgun sequence.
ACCESSION   DAZSBF010000658 DAZSBF010000000
VERSION     DAZSBF010000658.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277484
            Sequence Read Archive: SRR30464365
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396716.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396716.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 19:33:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,847
            CDSs (total)                      :: 4,756
            Genes (coding)                    :: 4,403
            CDSs (with protein)               :: 4,403
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 353
            CDSs (without protein)            :: 353
            Pseudo Genes (ambiguous residues) :: 0 of 353
            Pseudo Genes (frameshifted)       :: 152 of 353
            Pseudo Genes (incomplete)         :: 147 of 353
            Pseudo Genes (internal stop)      :: 110 of 353
            Pseudo Genes (multiple problems)  :: 53 of 353
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277484-rid23495443.guided.464"
                     /strain="M2002259"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-02-12"
                     /collected_by="FSS"
     gene            <1..668
                     /gene="erm"
                     /locus_tag="ACHXWD_004847"
     CDS             <1..668
                     /gene="erm"
                     /locus_tag="ACHXWD_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HFR7623446.1"
                     /translation="LNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSS
                     EKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIV
                     EEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPD
                     KYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFN
                     GRK"
BASE COUNT          469 a          231 c          224 g          394 t
ORIGIN      
        1 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
       61 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
      121 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
      181 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc
      241 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc
      301 tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
      361 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
      421 ctaaaccaag agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
      481 ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc
      541 aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa
      601 gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
      661 ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat
      721 gtagataaat tattaggtat actactgaca gcttccaagg agctaaagag gtccctagac
      781 tagcaagaag tacacaagaa gccttaaaga ttatagaaaa gcttttccat gaaatgctta
      841 acattattta atgttaacat gtggctttgt ggtaactacc acgagtggta acttgtaaca
      901 tgaaaaaagt tacaagttac aagtggttta tttacgaaca taaaggctag gaaagcactt
      961 gataagtata tcaagtgctt tttttccgtt cgattttact atccaagata ccgcttgacg
     1021 tacctaatat tttagtgtgc taagggcttt gctttttctg gttatagttc gttcaaaaaa
     1081 gaccgcaagg gtaaatgcac gatcatagat cgcccttgca gctttttaat ctctccactt
     1141 ttttataacc aagcaaacca ccaagcacac tgaaaaaata attaggaact aaagcgaagt
     1201 gcgtatcttg gataaatctt cactcgattt taaaagatta agcacccagc caaagcgcct
     1261 aaaaaaataa tttttttaaa gaaaggaagt aatttaaatg actacaattt taagcgac
//
